Package: dima-cli
DiMA - Diversity Motif AnalyserTable of ContentsWhat is DiMA?PublicationsInstallationBasic UsageShell CommandPythonResultsAdvance UsageShell CommandPythonResultsCommand-Line ArgumentsModule ParametersWhat is DiMA?Protein sequence diversity is one of the major challenges in the design of diagnostic, prophylactic and therapeutic
interventions against viruses. DiMA is a tool designed to facilitate the dissection of protein sequence diversity
dynamics for viruses. DiMA provides a quantitative measure of sequence diversity by use of Shannon’s entropy,
applied via a user-defined k-mer sliding window. Further, the entropy value is corrected for sample size bias by
applying a statistical adjustment.
Additionally, DiMA further interrogates the diversity by dissecting the entropy value at each k-mer position to various
diversity motifs. The distinct k-mer sequences at each position are classified into the following motifs based on
their incidence.Index: The predominant sequence.Major: The sequence with the second highest incidence after the Index.Minor: Kmers with incidence in between major and unique motifsUnique: Kmers which are only seen once in a particular kmer position.Moreover, the description line of the sequences in the alignment can be
formatted for inclusion of meta-data that can be tagged to the diversity motifs. DiMA enables comparative diversity
dynamics analysis, within and between proteins of a virus species, and proteomes of different viral species.Publicationshttps://arxiv.org/abs/2205.13915Installationpip install dima-cliBasic UsageShell Commanddima-cli-ialigned_sequences.afa-oresults.jsonPythonfromdimaimportDimaresults=Dima(sequences="aligned_sequences.afa").run()ResultsClick to view basic results{
  "sequence_count": 5,
  "support_threshold": 30,
  "low_support_count": 20,
  "query_name": "Unknown Query",
  "kmer_length": 9,
  "average_entropy": 0.06854034285524647,
  "highest_entropy": {
    "position": 186,
    "entropy": 1.3921472236645345
  },
  "results": [
    {
      "position": 1,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "MSASKEIKS",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        },
        {
          "sequence": "SAGVYMGNL",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        }
      ]
    },
    {
      "position": 2,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "AGVYMGNLS",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "SASKEIKSF",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        }
      ]
    },
    {
      "position": 3,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "GVYMGNLSS",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "ASKEIKSFL",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        }
      ]
    },
    {
      "position": 4,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "VYMGNLSSQ",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "SKEIKSFLW",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        }
      ]
    },
    {
      "position": 5,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "KEIKSFLWT",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        },
        {
          "sequence": "YMGNLSSQQ",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        }
      ]
    },
    {
      "position": 6,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "MGNLSSQQL",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "EIKSFLWTQ",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        }
      ]
    },
    {
      "position": 7,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "IKSFLWTQS",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        },
        {
          "sequence": "GNLSSQQLD",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        }
      ]
    },
    {
      "position": 8,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "KSFLWTQSL",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        },
        {
          "sequence": "NLSSQQLDQ",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        }
      ]
    },
    {
      "position": 9,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "SFLWTQSLR",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        },
        {
          "sequence": "LSSQQLDQR",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        }
      ]
    },
    {
      "position": 10,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "SSQQLDQRR",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "FLWTQSLRR",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        }
      ]
    },
    {
      "position": 11,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "LWTQSLRRE",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        },
        {
          "sequence": "SQQLDQRRA",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        }
      ]
    },
    {
      "position": 12,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "QQLDQRRAL",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "WTQSLRREL",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        }
      ]
    },
    {
      "position": 13,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "TQSLRRELS",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        },
        {
          "sequence": "QLDQRRALL",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        }
      ]
    },
    {
      "position": 14,
      "low_support": "LS",
      "entropy": 1.3709505944546687,
      "support": 5,
      "distinct_variants_count": 2,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 40.0,
      "diversity_motifs": [
        {
          "sequence": "QSLRRELSG",
          "count": 3,
          "incidence": 60.000004,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        },
        {
          "sequence": "LDQRRALLS",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "QSLRRELSS",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        }
      ]
    },
    {
      "position": 15,
      "low_support": "LS",
      "entropy": 1.3709505944546687,
      "support": 5,
      "distinct_variants_count": 2,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 40.0,
      "diversity_motifs": [
        {
          "sequence": "DQRRALLSM",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "SLRRELSGY",
          "count": 3,
          "incidence": 60.000004,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        },
        {
          "sequence": "SLRRELSSY",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        }
      ]
    },
    {
      "position": 16,
      "low_support": "LS",
      "entropy": 1.3709505944546687,
      "support": 5,
      "distinct_variants_count": 2,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 40.0,
      "diversity_motifs": [
        {
          "sequence": "QRRALLSMI",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "LRRELSSYC",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "LRRELSGYC",
          "count": 3,
          "incidence": 60.000004,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        }
      ]
    },
    {
      "position": 17,
      "low_support": "LS",
      "entropy": 1.3709505944546687,
      "support": 5,
      "distinct_variants_count": 2,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 40.0,
      "diversity_motifs": [
        {
          "sequence": "RRELSGYCS",
          "count": 3,
          "incidence": 60.000004,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        },
        {
          "sequence": "RRALLSMIG",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "RRELSSYCS",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        }
      ]
    },
    {
      "position": 18,
      "low_support": "LS",
      "entropy": 1.3709505944546687,
      "support": 5,
      "distinct_variants_count": 2,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 40.0,
      "diversity_motifs": [
        {
          "sequence": "RELSGYCSN",
          "count": 3,
          "incidence": 60.000004,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        },
        {
          "sequence": "RALLSMIGM",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "RELSSYCSN",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        }
      ]
    },
    {
      "position": 19,
      "low_support": "LS",
      "entropy": 1.3709505944546687,
      "support": 5,
      "distinct_variants_count": 2,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 40.0,
      "diversity_motifs": [
        {
          "sequence": "ALLSMIGMS",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "ELSSYCSNI",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "ELSGYCSNI",
          "count": 3,
          "incidence": 60.000004,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        }
      ]
    },
    {
      "position": 20,
      "low_support": "LS",
      "entropy": 1.3709505944546687,
      "support": 5,
      "distinct_variants_count": 2,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 40.0,
      "diversity_motifs": [
        {
          "sequence": "LSGYCSNIK",
          "count": 3,
          "incidence": 60.000004,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": null
        },
        {
          "sequence": "LLSMIGMSG",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        },
        {
          "sequence": "LSSYCSNIK",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": null
        }
      ]
    }
  ]
}Advance UsageShell Commanddima-cli-ialigned_sequences.afa-oresults.json-f"accession|strain|country|date"PythonfromdimaimportDimaresults=Dima(sequences="aligned_sequences.afa",header_format="accession|strain|country|date").run()ResultsClick to view advanced results{
  "sequence_count": 5,
  "support_threshold": 30,
  "low_support_count": 20,
  "query_name": "Unknown Query",
  "kmer_length": 9,
  "average_entropy": 0.06854034285524647,
  "highest_entropy": {
    "position": 186,
    "entropy": 1.3921472236645345
  },
  "results": [
    {
      "position": 1,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "MSASKEIKS",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": {
            "Accession": {
              "AYD75365.1": 1,
              "QEP52131.1": 1,
              "AYD75321.1": 1,
              "AYD75325.1": 1
            },
            "Country": {
              "Sierra Leone": 4
            },
            "Species": {
              "Homo sapiens": 3,
              "Unknown": 1
            },
            "Year": {
              "1977": 1,
              "2012": 1,
              "1980": 1,
              "1979": 1
            }
          }
        },
        {
          "sequence": "SAGVYMGNL",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": {
            "Accession": {
              "AYD75329.1": 1
            },
            "Country": {
              "Sierra Leone": 1
            },
            "Year": {
              "1975": 1
            },
            "Species": {
              "Homo sapiens": 1
            }
          }
        }
      ]
    },
    {
      "position": 2,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "SASKEIKSF",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": {
            "Species": {
              "Homo sapiens": 3,
              "Unknown": 1
            },
            "Year": {
              "1977": 1,
              "1980": 1,
              "1979": 1,
              "2012": 1
            },
            "Country": {
              "Sierra Leone": 4
            },
            "Accession": {
              "AYD75325.1": 1,
              "QEP52131.1": 1,
              "AYD75365.1": 1,
              "AYD75321.1": 1
            }
          }
        },
        {
          "sequence": "AGVYMGNLS",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": {
            "Species": {
              "Homo sapiens": 1
            },
            "Year": {
              "1975": 1
            },
            "Accession": {
              "AYD75329.1": 1
            },
            "Country": {
              "Sierra Leone": 1
            }
          }
        }
      ]
    },
    {
      "position": 3,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "ASKEIKSFL",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": {
            "Year": {
              "1980": 1,
              "1977": 1,
              "1979": 1,
              "2012": 1
            },
            "Accession": {
              "AYD75321.1": 1,
              "AYD75365.1": 1,
              "QEP52131.1": 1,
              "AYD75325.1": 1
            },
            "Country": {
              "Sierra Leone": 4
            },
            "Species": {
              "Unknown": 1,
              "Homo sapiens": 3
            }
          }
        },
        {
          "sequence": "GVYMGNLSS",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": {
            "Accession": {
              "AYD75329.1": 1
            },
            "Species": {
              "Homo sapiens": 1
            },
            "Country": {
              "Sierra Leone": 1
            },
            "Year": {
              "1975": 1
            }
          }
        }
      ]
    },
    {
      "position": 4,
      "low_support": "LS",
      "entropy": 0.7219280948873623,
      "support": 5,
      "distinct_variants_count": 1,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 20.0,
      "diversity_motifs": [
        {
          "sequence": "SKEIKSFLW",
          "count": 4,
          "incidence": 80.0,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": {
            "Accession": {
              "AYD75325.1": 1,
              "AYD75365.1": 1,
              "QEP52131.1": 1,
              "AYD75321.1": 1
            },
            "Country": {
              "Sierra Leone": 4
            },
            "Species": {
              "Unknown": 1,
              "Homo sapiens": 3
            },
            "Year": {
              "2012": 1,
              "1979": 1,
              "1980": 1,
              "1977": 1
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            },
            "Accession": {
              "AYD75365.1": 1,
              "AYD75325.1": 1,
              "AYD75321.1": 1
            },
            "Year": {
              "1977": 1,
              "1979": 1,
              "1980": 1
            }
          }
        },
        {
          "sequence": "ELSSYCSNI",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": {
            "Accession": {
              "QEP52131.1": 1
            },
            "Species": {
              "Homo sapiens": 1
            },
            "Country": {
              "Sierra Leone": 1
            },
            "Year": {
              "2012": 1
            }
          }
        },
        {
          "sequence": "ALLSMIGMS",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": {
            "Country": {
              "Sierra Leone": 1
            },
            "Accession": {
              "AYD75329.1": 1
            },
            "Species": {
              "Homo sapiens": 1
            },
            "Year": {
              "1975": 1
            }
          }
        }
      ]
    },
    {
      "position": 20,
      "low_support": "LS",
      "entropy": 1.3709505944546687,
      "support": 5,
      "distinct_variants_count": 2,
      "distinct_variants_incidence": 100.0,
      "total_variants_incidence": 40.0,
      "diversity_motifs": [
        {
          "sequence": "LLSMIGMSG",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": {
            "Accession": {
              "AYD75329.1": 1
            },
            "Country": {
              "Sierra Leone": 1
            },
            "Species": {
              "Homo sapiens": 1
            },
            "Year": {
              "1975": 1
            }
          }
        },
        {
          "sequence": "LSGYCSNIK",
          "count": 3,
          "incidence": 60.000004,
          "motif_short": "I",
          "motif_long": "Index",
          "metadata": {
            "Country": {
              "Sierra Leone": 3
            },
            "Year": {
              "1979": 1,
              "1977": 1,
              "1980": 1
            },
            "Accession": {
              "AYD75365.1": 1,
              "AYD75321.1": 1,
              "AYD75325.1": 1
            },
            "Species": {
              "Unknown": 1,
              "Homo sapiens": 2
            }
          }
        },
        {
          "sequence": "LSSYCSNIK",
          "count": 1,
          "incidence": 20.0,
          "motif_short": "U",
          "motif_long": "Unique",
          "metadata": {
            "Year": {
              "2012": 1
            },
            "Species": {
              "Homo sapiens": 1
            },
            "Accession": {
              "QEP52131.1": 1
            },
            "Country": {
              "Sierra Leone": 1
            }
          }
        }
      ]
    }
  ]
}Command-Line ArgumentsArgumentTypeRequiredDefaultExampleDescription-hN/AFalseN/Adima-cli -hPrints a summary of all available command-line arguments.-nStringFalseUnknowndima-cli -i sequences.afa -o results.json -f "accession|strain|country" -n "NA"-n "Unknown"Silently fix missing values in the FASTA header with given value.-vN/AFalseN/Adima-cli -vPrints the version of dima-cli that is currently installed.-qStringFalseUnknown Querydima-cli -q "Coronavirus Surface Protein" -i sequences.afa -o results.jsonThe name of the sample that will appear on the results.-iStringTrueN/Adima-cli -i sequences.afa -o results.jsonThe path to the FASTA Multiple Sequence Alignment file.-oStringTrueN/Adima-cli -i sequences.afa -o results,jsonThe location where the results shall be saved.-lIntegerFalse9dima-cli -i sequences.afa -l 12 -o results.jsonThe length of the kmers generated.-fStringFalseN/Adima-cli -i sequences.afa -f "accession|strain|country" -o results.jsonThe format of the FASTA header. Labels where each variant of a kmer position originated from.-sIntegerFalse30dima-cli -i sequences.afa -l 12 -s 40  -o results.jsonThe minimum required support for each kmer position.-anucleotide/proteinFalseproteindima-cli -i dna_sequences.afa -a nucleotide -o results.jsonThe alphabet of the sequences (ie:protein/nucleotide, default: protein)-tjson/xlsxFalsejsondima-cli -i dna_sequences.afa -a nucleotide -o results.json -t xlsxThe output format (ie:json/xlsx, default: json)-cStringFalseN/Adima-cli -i dna_sequences.afa -a nucleotide -o results.json -c hcs.jsonPath to save Highly Conserved Sequences (HCS) in JSON format.-eFloatFalse100dima-cli -i dna_sequences.afa -a nucleotide -o results.json -c hcs.json -e 90.5Minimum incidence (%) threshold for HCS concatenation.Module ParametersParameterTypeRequiredDefaultDescriptionsequencesString/StringIOTrueN/AThe path to a FASTA Multiple Sequence Alignment file (MSA), or a StringIO object containing FASTA MSA.kmer_lengthIntegerFalse9The length of the kmers generated.header_fillnaStringFalseUnknownSilently fix missing values in the FASTA header with given value (only required whenheader_formatis given).header_formatStringFalseN/AThe format of the FASTA header. Labels where each variant of a kmer position originated from.support_thresholdIntegerFalse30The minimum required support for each kmer position.query_nameStringFalseUnknown QueryThe name of the sample that will appear on the results.alphabetStringFalseproteinThe alphabet of the sequences (ie: protein/nucleotide, default: protein)