Samantha912/NLP_A2_WTH huggingface dataset:
TAE buffer is a buffer solution containing a mixture of Tris base, acetic acid and EDTA.  

In molecular biology, it is used in agarose electrophoresis typically for the separation of nucleic acids such as DNA and RNA. It is made up of Tris-acetate buffer, usually at pH 8.3, and EDTA, which sequesters divalent cations. TAE has a lower buffer capacity than TBE and can easily become exhausted, but linear, double stranded DNA runs faster in TAE.

Previously, Brody & Kern simplified electrophoretic buffers by substituting TBE and TAE buffers for a more efficient and inexpensive conductive media in gel systems.

Uses
TAE (Tris-acetate-EDTA) buffer is used as both a running buffer and in agarose gels. Its use in denaturing gradient gel electrophoresis methods for broad-range mutation analysis has also been described. TAE has been used at various concentrations to study the mobility of DNA in solution with and without sodium chloride. However, high concentrations of sodium chloride (and many other salts) in a DNA sample retard its mobility. This may lead to incorrect interpretations of the resulting DNA banding pattern.

Preparation
TAE buffer is commonly prepared as a 50× stock solution for laboratory use.  A 50× stock solution can be prepared by dissolving 242 g Tris base in water, adding 57.1 ml glacial acetic acid, and 100 ml of 500 mM EDTA (pH 8.0) solution, and bringing the final volume up to 1 litre. This stock solution can be diluted 49:1 with water to make a 1× working solution. This 1× solution will contain 40 mM Tris, 20 mM acetic acid, and 1 mM EDTA.

2 M = 2000 mM so 2000 mM /50 = 40 mM for 1×.  1M = 1000 mM so 1000 mM /50 = 20 mM for 1×.  50 mM /50 = 1 mM for 1×.

First of all, these ingredients should be dissolved in 500 ml, then made up to 1000 ml.  Note: EDTA will take more time to dissolve, so while dissolving EDTA use magnetic stirrer (few amounts of EDTA in 3 or 4 times).

A step-by-step recipe of the preparation method for 50× TAE buffer is available on protocols.io.

See also
 TBE buffer
 LB buffer

References

__notoc__
Buffer solutions
Those High Grey Walls is a 1939 American crime film directed by Charles Vidor and starring Walter Connolly, Onslow Stevens and Iris Meredith. Produced and distributed by Columbia Pictures, it is also known by the alternative title The Gates of Alcatraz.

Plot
Dr. MacAuley, a kindly beloved country doctor, is sent to Fillmore Prison. His Crime was for removing a bullet from a young man who was escaping from the police.

Cast
 Walter Connolly as Dr. MacAuley
 Onslow Stevens as Dr. Frank Norton
 Paul Fix as Nightingale
 Bernard Nedell as Redlands
 Iris Meredith as Mary MacAuley
 Oscar O'Shea as Warden
 Nicholas Soussanin as "Lindy" Lindstrom
 Don Beddoe as Jockey

References

External links

American crime drama films
Columbia Pictures films
Films directed by Charles Vidor
Films set in prison
1939 crime drama films
American black-and-white films
1939 films
1930s American films
1930s English-language films
KLLT was a radio station on 95.3 MHz in Grants, New Mexico, that operated between April 16, 1980, and July 27, 1988. The station's deep indebtedness, combined with a refusal of permission to build a mountaintop tower and move into the Albuquerque radio market, led the station to cease operations.

History

On October 2, 1979, the Grants Broadcasting Company was granted a construction permit to build a new radio station on 95.3 in Grants, with a transmitter  southwest of the city. KYKN signed on April 16, 1980, with a country format.

The Rainbow Broadcasting Corporation of Denver, owned by Michelle Z. Elliot, acquired KYKN in 1983 for $115,000. The new ownership changed the station's call letters to KLLT on October 31, though the country format remained. However, Rainbow ownership frequently changed hands. In 1984, Robert and Maxine Hedin acquired control of the company, and in 1986, Steven Humphries bought the entirety of Rainbow. By this time, KLLT, which had changed to contemporary hit radio earlier in the year, was deep in debt. Humphries agreed to buy the station for the assumption of its $175,000 in debt, and when he discovered another $125,000, he immediately filed for Chapter 11 bankruptcy reorganization.

Given the station's financial situation and limited facility covering Grants, the station sought a coverage and frequency upgrade as part of a program to move into Albuquerque. In February 1988, the Federal Communications Commission approved an application to move KLLT to 103.7 MHz and increase power to 50 kW. However, the United States Forest Service refused on two occasions to grant KLLT a land lease to construct the tower atop Mount Taylor that was necessary to carry out the improvements. With sales revenues continuing to decline, Humphries opted to take KLLT off the air on July 27, 1988.

However, KLLT's license continued to exist after Humphries took the station silent, a fact that would prove crucial to the course of events in another proceeding. Just days later, on August 2, Humphries filed to buy Santa Fe's KNMQ. The sale application prompted a petition to deny from Fairmont Communications Corp., owners of KKOB and KKOB-FM, which alleged that Humphries had put KLLT in bankruptcy the same day he had filed to buy the Santa Fe station, and that Humphries could not own both FM stations due to impermissible signal overlap. Humphries contended that Fairmont was upset that KNMQ had lured top-rated morning show host Paul Sisneros from KKOB, and that the overlap between the two stations would only have occurred if KLLT had been moved to Mount Taylor. In late January 1989, the FCC dismissed the transfer application, primarily on the signal overlap issue; Humphries filed a petition for reconsideration, claiming that with the station in bankruptcy, it was now the property of the bankruptcy trustee, but the deal took so long to complete that the application was withdrawn altogether.

References

External links

LLT
Radio stations established in 1980
Radio stations disestablished in 1988
Defunct radio stations in the United States
1980 establishments in New Mexico
1988 disestablishments in New Mexico
LLT
Securicula gora is a species of cyprinid fish found in Bangladesh, India, Pakistan and possibly in Nepal.  It is the only species in its genus.

Description
'Sericula gora is a silvery fish with an upward pointing mouth, which has a prominent cartilagenous knob on the lower jaw, and a keel running along the abdomen from below the pectoral fin to the anal fin. The dorsal fin has its origin in front of that of the anal fin. The caudal fin has its lower lobe longer than the upper lobe and the pelvic fins has well developed axillary scales. There are 140-160 scales in the lateral line which is complete and decurved. It grows to a maximum length of 24.5cm.

Distribution
Securicula gora is known to occur in the Ganges and Brahmaputra drainages of India, Bangladesh and Nepal. The presence of this species in the Punjab in India and Pakistan has not been confirmed.

Habitat and ecology
Securicula gora is a pelagic, freshwater species which can be found in rivers, beels and canals. It is a predatory species which feeds on insects, insect larvae and crustaceans which it takes at or near the surface of the water. In 1988 a new species of monogenean parasite, Heteromazocraes mamaevi was described from specimens of S. gora sampled near Lucknow, until then this genus of parasites had been thought only to occur on members of the Clupeid family Engraulidae.

Conservation and Human use
Securicula gora is a common and widespread species where it occurs and is thus treated as Least Concern by the IUCN but its habitat can be affected by silting caused by logging and consequent deforestation of the land surrounding the waters it inhabits.

This species is consumed as food in Bangladesh and other parts of it range where it is normally of minor interest to commercial fisheries,
 except in parts of India where it is commercially fished for.

References

Danios
Monotypic ray-finned fish genera
Fish of Asia
Fish of India
Fish of Pakistan
Fish of Bangladesh
Taxa named by Albert Günther
Hung Shing wong (), also known as Hung Shing Ye () and Tai Wong () is a Chinese folk religion deity. The most popular tale states that in his lifetime he was a government official in the Tang dynasty (AD 618–907) named Hung Hei () serving Pun Yue in present-day Guangdong, China.

His festival () is held on the 13th day of the 2nd month in the Chinese calendar.

Life and memory
The popular tale is that Hung Hei was a righteous government official who won approbation from the people. During his tenure in office, he promoted the study and application of astronomy, geography and mathematics, and established an observatory to observe the meteorological changes, thus contributing to the well-being of people under his governance, especially fishermen and sea traders. Unfortunately, he died young.

After his death, an Emperor of the Tang dynasty disseminated his virtues to the whole country and bestowed upon him the posthumous title of Nam Hoi Kwong Li Hung Shing Tai Wong (), lit. the Saint King Hung the Widely Beneficial of South Sea.  It is usually shortened to Hung Shing or Tai Wong.

Legend has it that Hung Shing continued to guard the people against natural disasters on numerous occasions after his death, and showed his presence to save many people during tempests. The government as well as fishermen in the surrounding area built many temples to worship him as the God of Southern Sea. Hung Shing temples have been widely built in southern China, especially Guangdong province and in Hong Kong. In Hong Kong, they are named Hung Shing Miu () or Tai Wong Miu ().

Modern scholars however believe that Hung Shing is derived from the worship of Zhurong, the fire deity and god of the southern seas. Worship of Zhurong as the god of the southern seas predates the Tang dynasty: one temple dedicated to the "South Sea God" in Guangzhou dates from the preceding Sui dynasty. The title "South Sea God" was conferred by Emperor Wen of Sui. Emperor Xuanzong of Tang conferred on the South Sea God the title "Guangli DaWang" (), and additional titles were conferred during the Song and Yuan dynasties, resulting eventually in the full title "South Sea Guangli Hongsheng Zhaoshun Weixian Lingfu Wang" ().

See also
 Hung Shing Temple for a list of temples dedicated to Hung Shing
 Tin Hau and Tin Hau temples in Hong Kong
 Tam Kung and His temples in Hong Kong & overseas
 Yiu Ming Temple, New South Wales, Australia

References

Chinese gods
Tang dynasty people
Taoism in Guangdong
Deified Chinese people
Mohammed Khair-Eddine (; ) (1941 – November 18, 1995) was a Moroccan poet and writer. He was among the most famous Moroccan Berber literary figures of the 20th century.

Life
Mohammed Khaïr-Eddine was born in Tafraout, a Berber town in the Souss-Massa-Drâa region (Tiznit province), in the south of Morocco, 180 km south of Agadir.

As a young writer, he joined the circle of writers known as  the Amitiés littéraires et artistiques in Casablanca. In 1964 Khair-Eddir founded the "Poésie Toute" movement. In 1965 he was exiled to France where he spent years working in factories. In 1967 he started publishing again, writing for "Lettres nouvelles" and "Présence africaine". Mohammed Khair-Eddine returned to Morocco in 1979.

Khair-Eddine died in Rabat November 18, 1995.

Selected works
Agadir (1967): The author is very much taken by the "séisme" of 1960, he moves to Agadir in 1961 and stays there until 1963.
Résurrection des fleurs sauvages (Éditions Stouky, Rabat, 1981).
Légende et vie d' Agoun'chich (Le Seuil, 1984).

Éditions du Seuil 
For the most part his works have been published by Éditions du Seuil:
Corps négatif
Histoire d'un Bon Dieu
Soleil arachnide
Moi l'aigre
Le Déterreur
Ce Maroc!
Une odeur de manthèque
Une vie, un rêve, un peuple 
Toujours errants
Légende et vie d'Agoun'chich
Résurrection des fleurs sauvages

External links
  limag.refer.org

References
 

Berber poets
Moroccan male poets
Moroccan writers
1941 births
1995 deaths
Moroccan writers in French
20th-century Moroccan poets
People from Tafraout
20th-century poets
Shilha people
Haksar is a Kashmiri Pandit surname and clan. They are native to the Kashmir Valley within the Indian state of Jammu and Kashmir and they have a long tradition of Indian administrative service based on fluency in a link language - Persian under the Mughals and English under the British. In light of this fact, the Haksar family historically became a prominent administrative family in other parts of India, namely in Indore and Gwalior.

Notable people
Sir Kailash Narain Haksar (1878–1953), Indian minister 
P. N. Haksar (1913–1998), Indian minister
Ajit Narain Haksar (1925–2005), Indian businessman
A. N. D. Haksar (born 1933), Indian diplomat and prominent translator of Sanskrit classics

References

Kashmiri tribes
Kashmiri-language surnames
Indian surnames
Hindu surnames
Social groups of Jammu and Kashmir
Moses Fellows (November 7, 1803 – September 25, 1879) was an American politician who served as the fourth mayor of Manchester, New Hampshire.

Fellows was born to Simon and Dorthy (Bartlett) Fellows in Brentwood, New Hampshire, on November 7, 1803.

While he lived in Brentwood, until he resigned in 1827, Fellows was a sergeant in the New Hampshire Militia.

Fellows was Chairman of the Manchester Board of Selectmen in 1842–1843 and 1846, also in 1846 a member of the Manchester board of Aldermen, and a member of the New Hampshire House of Representatives in 1847–1848.

In 1850-1851 Fellows was the mayor of Manchester, New Hampshire, having been elected in the city's 1850 and 1851 mayoral elections.

Death
Fellows died on September 25, 1879.

Notes

 

1803 births
1879 deaths
Mayors of Manchester, New Hampshire
Members of the New Hampshire House of Representatives
19th-century American politicians
People from Brentwood, New Hampshire
An Islamic Utopian: A Political Biography of Ali Shariati is a 1998 book by Ali Rahnema in which the author examines the life and works of Ali Shariati. It has been translated into Persian, Arabic, Turkish and Indonesian. This book is the first close study of Ali Shariati in English.

Reception
The book has already received positive reviews by Donna Robinson Divine, Vanessa Martin and Behrooz Ghamari‐Tabrizi

References

External links 
 An Islamic Utopian

1998 non-fiction books
2000 non-fiction books
English-language books
Iranian biographies
Books about politics of Iran
Bloomsbury Publishing books
Works about Ali Shariati
I.B. Tauris books
The Kango (Bakango), also known as the Batchua and Mbuti-Sua, are an Mbuti pygmy people of the Ituri forest. They speak a Bantu language, Bila, apparently in two dialects, northern Sua and southern Kango.

They are in a patron–vassal relationship with several Bantu-speaking peoples, the Bila, Budu, Ndaka, Bombo, Liko, and Baali; two Sudanic peoples, the Lese and Luumbi, and the Ubangian Mayogo.

They may be the Wochua people described in the 19th century.

References
Serge BAHUCHET, 2006. "Languages of the African Rainforest « Pygmy » Hunter-Gatherers: Language Shifts without Cultural Admixture." In Historical linguistics and hunter-gatherers populations in global perspective. Leipzig.

African Pygmies
Ethnic groups in the Democratic Republic of the Congo
A menntaskóli () is an Icelandic equivalent to a gymnasium (high school, or college), although it works differently from other countries.

In Iceland grade school (grunnskóli) starts in 1st grade at the age of 6 and ends in 10th grade at the age of 16, which are the only mandatory school years in Iceland. After graduation from grade school, students choose what High Schools/College they wish to go to (this is similar to choosing a college in the United States). It is all based on what students wish to learn. It is a 3-year term; roughly the equivalent of the last three years of high school and the first year of university in the United States. A completion of menntaskóli usually provides the student with stúdentspróf (upper secondary certificate) which is required for entry to most university programs in Iceland. Stúdentspróf is somewhat equivalent to a JR college diploma in the US.

List of Icelandic Secondary Schools
 Borgarholtsskóli (Borgó) (Reykjavík)
 Fjölbrautaskóli Vesturlands á Akranesi (FVA) (Akranes)
 Fjölbrautaskóli Norðurlands Vestra (FNV) (Sauðárkrókur)
 Fjölbrautaskóli Snæfellinga (FSN) (Grundarfjörður)
 Fjölbrautaskóli Suðurlands (FSu) (Selfoss)
 Fjölbrautaskóli Suðurnesja (FS) (Reykjanesbær)
 Fjölbrautaskólinn í Breiðholti (FB) (Reykjavík)
 Fjölbrautaskólinn í Garðabæ (FG) (Garðabær)
 Fjölbrautaskólinn við Ármúla (FÁ) (Reykjavík)
 Flensborgarskóli (Hafnarfjörður)
 Framhaldsskólinn á Húsavík (FSH) (Húsavík)
 Framhaldsskólinn á Laugum (Laugar)
 Framhaldsskólinn í Austur-Skaftafellssýslu (FAS) (Höfn)
 Framhaldsskólinn í Mosfellsbæ (FMOS) (Mosfellsbær)
 Framhaldsskólinn í Vestmannaeyjum (FÍV) (Vestmannaeyjar)
 Kvennaskóli Íslands (Kvennó) (Reykjavík)
 Menntaskólinn á Akureyri (MA) (Akureyri)
 Menntaskólinn við Hamrahlíð (MH) (Reykjavík)
 Menntaskólinn við Sund (MS) (Reykjavík)
 Menntaskóli Borgarfjarðar (MB) (Borgarnes)
 Menntaskólinn á Ásbrú (MÁ) (Reykjanesbær)
 Menntaskólinn á Ísafirði (MÍ) (Ísafjörður)
 Menntaskólinn á Egilsstöðum (ME) (Egilsstaðir)
 Menntaskólinn á Tröllaskaga (MTR) (Ólafsfjörður)
 Menntaskólinn í Reykjavík (MR) (Reykjavík)
 Menntaskólinn að Laugarvatni (ML) (Laugarvatn)
 Menntaskólinn í Kópavogi (MK) (Kópavogur)
 Tækniskólinn (Reykjavík)
 Verkmenntaskóli Austurlands (VA) (Neskaupstaður)
 Verkmenntaskólinn á Akureyri (VMA) (Akureyri)
 Verzlunarskóli Íslands (Verzló), (Reykjavík)

School types
Education in Iceland
Zaleya is a genus of flowering plants in the iceplant family Aizoaceae, found in Cape Verde, Africa, Madagascar, Réunion, Socotra, West Asia, the Indian Subcontinent, Sri Lanka, Myanmar, and Australia.

Species
Currently accepted species include:
Zaleya camillei (Cordem.) H.E.K.Hartmann
Zaleya decandra (L.) Burm.f.
Zaleya galericulata (Melville) H.Eichler
Zaleya govindia (Buch.-Ham. ex G.Don) N.C.Nair
Zaleya pentandra (L.) C.Jeffrey
Zaleya redimita (Melville) Bhandari

References

Aizoaceae
Aizoaceae genera
Taxa named by Nicolaas Laurens Burman
Detective 909 Keralathil is a 1970 Indian Malayalam-language spy film directed by P. Venu and produced by T. C. Sankar. The film stars Jayabharathi, KP Ummer, Sudheer, Vijayasree, Hemalatha, Sankaradi and Sreelatha Namboothiri. The film had musical score by M. K. Arjunan.

Plot

Cast 

Jayabharathi
K. P. Ummer
Kottarakkara Sreedharan Nair
Sudheer
Vijayasree
Hemalatha
Sankaradi
Sreelatha Namboothiri
T. S. Muthaiah
Sadhana
Abbas
Alummoodan
Girish Kumar
Junior Sheela
Kshema
Kuttan Pillai
M. S. Namboothiri
Meena
Nambiar
Panjabi
Ramu
Shamsudeen
Shyam Shankar
Sreekumar

Soundtrack 
The music was composed by M. K. Arjunan and the lyrics were written by P. Bhaskaran.

References

External links 
 

1970 films
1970s Malayalam-language films
1970s spy films
Films directed by P. Venu
Indian spy films
First Offence is the debut album of London-based 'wildstyle' band King Prawn. It was released in 1995, and also re-released in 2001.

Track listing
"Doledrums"
"Salvation" 
"Boxed & Packaged"
"Immigrant Song Too"
"Restart (Acts I & II)"
"Bossman's Bleeding"
"Nobody Like You"
"Alien Spawn"
"First Defence"

Re-released track listing
"Immigrant Song Too"
"First Defence"
"Boxed & Packaged"
"Bossman's Bleeding"
"Restart" (Acts I & II)
"Doledrums"
"Nobody Like You"
"Salvation"
"Alien Spawn"
"Poison in the Air" (Alternative Mix)
"Divine Badness" (Alternative Mix)
"Holy War" (Live Studio Recording)
"Winning Again" (Live Studio Recording)
"Buried Alive" (Demo Recording)
"Poison in the Air" (Enhanced Video)

External links
myspace.com/originalkingprawn
Golf Records profile of King Prawn
King Prawn interview

1995 debut albums
King Prawn (band) albums
Pachytodes is a genus of beetle belonging to the family Cerambycidae, subfamily Lepturinae (flower longhorns).

Species
Species within this genus include:
 Pachytodes cerambyciformis (Schrank, 1781)
 Pachytodes cometes (Bates, 1884)
 Pachytodes erraticus (Dalman, 1817)
 Pachytodes longipes (Gebler, 1832)
 Pachytodes orthotrichus (Plavilstshikov, 1936)

References 

Lepturinae
Martyn Griffiths (born 18 April 1946) is a British racing driver, whose greatest success has been in hillclimbing. He has won the British Hill Climb Championship on five occasions (1979, 1986, 1987, 1990, 1991).

References

British hillclimb drivers
Living people
1946 births
"Wasn't Expecting That" is a song recorded by British singer-songwriter Jamie Lawson. It was originally released on 11 March 2011 as the lead single from his third studio album Wasn't Expecting That (2011) and peaked at number 3 on the Irish Singles Chart. Lawson was the first artist signed by Ed Sheeran to his new record label, Gingerbread Man Recordings in 2015. The song was re-released on 3 April 2015 as the lead single from his fourth studio album Jamie Lawson. The song peaked at number 6 on the UK Singles Chart. On 31 July 2016, Lawson performed the song in an episode of the Australian soap Neighbours.

Music video
Three variations of the music video were made: the original, an international version and an Australian version.

The original version of the song was posted as an acoustic performance to YouTube in January 2011 and now has over 1 million views. 

A music video to accompany the new recording for re-release was posted to YouTube on 26 August 2015 at a total length of three minutes and twenty-eight seconds. The video, directed by award-winning filmmaker Bouha Kazmi, has more than 11 million views on YouTube. The video tells the story of a couple who fall in love, but are then left traumatised when the woman is diagnosed with terminal cancer. It follows the couple as they go back in time throughout their relationship. It shows the woman giving birth, the two getting married and their first kiss. 

A further version was filmed in Sydney for the Australian market when Lawson toured with Ed Sheeran in Australia in 2015.

Charts

Weekly charts

Year-end charts

Certifications

Release history

References

External links
 
 Australian version of the music video on Vimeo

2011 singles
2015 singles
2011 songs
Jamie Lawson (musician) songs
Gingerbread Man Records singles
Songs written by Jamie Lawson (musician)