Samantha912/NLP_A2_WTH huggingface dataset:
The pGreen plasmids are vectors for plant transformation. They were first described in 2000 as components of a novel T-DNA binary system. The supporting web page provides supplementary information and ongoing support to researchers to request their plasmid resources. As these plasmids have been taken up by the research community, the plasmids have been developed, expanding the resources available to the community.

Researchers are encouraged to contribute to this research community by submitting their vector sequence to genbank and providing a description of the plasmid on the site.

pGreenI and pGreenII
pGreen is the original pGreen plasmid. pGreenII features plasmid backbone modification to improve plasmid stability.

T-DNA regions

No transformation selection

pGreenII 0000: minimal T-DNA with Left and Right border, lacZ gene for blue/white selection during cloning multiple cloning site derived from pBluescript.

pGreenII 62-SK: derived from pGreenII 0000, the LacZ blue/white cloning selection has been replaced with a 35S-MCS-CaMV cassette that allows the insertion of a gene of interest into a 35S over-expression cassette. The multiple cloning site (MCS) is derived from pBluescript.

Kanamycin selection
pGreenII 0029: derived from pGreenII 0000, a nos-kan cassette has been inserted into the HpaI site of the Left Border, providing resistance to kanamycin during plant transformation selection.

pGreenII 0029 62-SK: derived from pGreenII 0029, the LacZ blue/white cloning selection has been replaced with a 35S-MCS-CaMV cassette that allows the insertion of a gene of interest into a 35S over-expression cassette. The MCS is derived from pBluescript.

Hygromycin selection

pGreenII 0179: derived from pGreenII 0000, a 35S-hyg cassette has been inserted into the HpaI site of the Left Border, providing resistance to hygromycin during plant transformation selection.

Bialaphos selection

pGreenII 0229: derived from pGreenII 0000, a nos-bar cassette has been inserted into the HpaI site of the Left Border, providing resistance to bialaphos or phosphinothricin during plant transformation selection.

pGreenII 0229 62-SK: derived from pGreenII 0229, the LacZ blue/white cloning selection has been replaced with a 35S-MCS-CaMV cassette that allows the insertion of a gene of interest into a 35S over-expression cassette. The MCS is derived from pBluescript.

pSoup
This is the helper plasmid that provides the replicase function for the pSa replication origin of pGreen. pSoup is tetracyclin resistant and a complementary incompatibility group such that it can co-exist with pGreen in the Agrobacterium cell.

pSoup: the original help plasmid for pGreen. pGreen will not replicate in Agrobacterium if it is not present.

References

External links
 The pGreen research page

Biotechnology
Pick Me Up is the second studio album by Canadian country music artist Brett Kissel. It was released on September 11, 2015, via Warner Music Canada. The album includes the number one single "Airwaves".

Upon release, Pick Me Up debuted at number seven on the Canadian Albums Chart.

Promotion
In the fall of 2014, Kissel opened for Brad Paisley on the Canadian leg of his then-current tour and began playing material from what would become Pick Me Up. Kissel will headline "The Airwaves Tour 2015" in promotion of Pick Me Up beginning on October 10 in Simcoe, Ontario. Dates were announced through to November 28, with additional stops to be added. He also performed at the Grand Ole Opry on August 29, 2015.

Singles
On June 19, 2015, "Airwaves" was released to digital retailers and Canadian country radio as the album's lead single. It entered the Billboard Canada Country airplay chart at number 31 as the most-added single of the week and reached the number one position on the chart dated October 3, 2015. It has also charted on the Billboard Canadian Hot 100 at number 61.

Track listing

Credits and personnel
Credits adapted from liner notes.

Recorded and engineered at
 Nashville, Tennessee 
 Saskatoon, Saskatchewan 
Mastered at
 Nashville, Tennessee 

Performance credits
 All vocals – Brett Kissel
 Featured vocas – Carolyn Dawn Johnson
 Background vocals – Mickey Jack Cones, Matty McKay, Brett Kissel, Russell Terrell, the Good Friday Choir

Instruments

 Bass – Mark Hill, Justin Kudding, Derek Stremel
 Drums – Chad Malchert, Nir Z
 Fiddle – Tyler Vollrath
 Guitars  – Mickey Jack Cones, J.T. Corenflos, Hunter Hayes, Jeff King, Brett Kissel, Troy Lancaster, B. James Lowry, Matty McKay

 Mandolin – Troy Lancaster, Matty McKay
 Piano and synthesizer – Mickey Jack Cones, Tony Harrell, Bart McKay
 Percussion – Mickey Jack Cones
 Steel guitar – Randle Currie, Mike Johnson

Production

 Engineers – Mickey Jack Cones, Bart McKay, Dave Salley
 Assistant engineers – Brady Tilow
 Management – Louis O'Reilly, Invictus Entertainment (Canada); Bob Doyle, Major Bob Music (US)
 Management Agency – Jim Cressman, Invictus Entertainment (IEG)
 Mastering – Andrew Mendelson
 Mixers – Mickey Jack Cones, Bart McKay, Brett Kissel
 Packaging designer – Patrick Duffy for Attention

 Photographer – Juan Pont Lezica
 Record producer – Mickey Jack Cones, Bart McKay, Brett Kissel
 Programming and editing – Mickey Jack Cones, Brady Tilow
 Songwriters – Cary Barlowe, Casey Beathard, Rachel Bradshaw, Chase Bryant, Jacob Bryant, Zach Crowell, John Davidson, Adrienne Follesé, Keith Follesé, Ashley Gorley, Ted Hewitt, Kyle Jacobs, Matt Jenkins, Brett Kissel, Hillary Lindsey, Tony Martin, Jake Mitchell, Jason Mizelle, Billy Montana, Phil O'Donnell, Troy Olsen, Gordie Sampson, Jonathan Singleton, Neil Thrasher, JP Williams

 Additional credits
 Hunter Hayes appears courtesy of Atlantic Recording Corporation
 Carolyn Dawn Johnson appears courtesy of Dancing Lily Music

Chart performance

Album

Singles

References

2015 albums
Brett Kissel albums
Warner Music Group albums
Iowa Highway 210 (Iowa 210) is an east–west highway in central Iowa covering .  It begins at Iowa Highway 141 south of Woodward and ends at U.S. Highway 65 south of Collins.  The routing closely parallels and crosses the former Chicago, Milwaukee, St. Paul and Pacific Railroad grade.  Passing through mostly rural farmland, Iowa 210 serves as a collector for two central Iowa freeways — Interstate 35 and Iowa 141.

Route description
Iowa Highway 210 begins at an interchange with Iowa Highway 141 south of Woodward.  Just north of Woodward, and immediately after crossing into Boone County, Iowa 210 turns east at the entrance to the Woodward Resource Center.  The route crosses the meandering Des Moines River next to the former Milwaukee Road high bridge before entering Madrid.  At Madrid, Iowa 210 shares one block, , with Iowa Highway 17.

Iowa Highway 210 continues east and enters Story County and the town of Slater.  South of Huxley, Iowa 210 intersects U.S. Highway 69.  East of US 69, Iowa 210 serves as a collector route to Interstate 35, serving, on average, over 4500 vehicles per day.  East of Interstate 35, Iowa Highway 210 primarily serves local traffic.  Iowa 210 serves Cambridge via county highways and Maxwell directly.  South of Collins, Iowa 210 ends at U.S. Highway 65.

History
Iowa Highway 210 was designated on January 8, 1938 as a double-spur route between Slater and Maxwell.  It was extended eastward to US 65 and westward to Iowa 141, absorbing Iowa 89, in November 1980.  In 1997, when Iowa 141 was upgraded to an expressway, the southern end of Iowa 210 was extended approximately .  A mile marker 1A exists near mile marker 1 to account for the addition.

Major intersections

Related route

Iowa 89 began at an intersection with Iowa Highway 141 south of Woodward and headed north into the town. Just north of Woodward, and immediately after crossing into Boone County, Iowa 89 turned east at the entrance to the Woodward Resource Center. The route crossed the Des Moines River next to the Milwaukee Road high bridge before entering Madrid. At Madrid, Iowa 89 ended at Iowa 17.  Iowa 89 was absorbed into Iowa 210 in 1980.

References

External links

202
Convertible Music is the 1982 new wave debut album by pop rock artist Josie Cotton, released on Elektra Records.

Convertible Music contained Cotton's two more well-known songs, the minor hits "Johnny Are You Queer?" and "He Could Be the One", both of which were performed by Cotton in the 1983 movie Valley Girl as well as appeared on the film's hit soundtrack (#155, Billboard 200).

Track listing
"He Could Be the One" (Paine, Paine) – 2:48
"Rockin' Love" (Paine, Paine) – 3:02
"Waitin' for Your Love" (Cotton) – 3:21
"So Close" (Cotton) – 2:39
"I Need the Night, Tonight" (Cotton) – 3:12
"Johnny Are You Queer?" (Paine, Paine) – 2:46
"Systematic Way" (Cotton) – 2:57
"Another Girl" (Cotton) – 3:14
"Bye, Bye Baby" (Cotton) – 2:57
"No Pictures of Dad" (Giltridge) – 3:33
"Tell Him" (Russell) – 2:36

Personnel
Josie Cotton – vocals
J. B. Frank – keyboards
Gary Ferguson – drums
Pete McRae – guitar
Bobby Paine – guitar, bass guitar

References

Josie Cotton albums
1982 debut albums
Elektra Records albums
Albums produced by Roy Thomas Baker
Sparta/Fort McCoy Airport  is a public and military use airport located in Monroe County, Wisconsin, United States, three nautical miles (6 km) northeast of Sparta. The airport is used heavily by the U.S. Army, accounting for 75% of traffic operations, primarily transient military aircraft supporting operations at nearby Fort McCoy, which is a major training facility. It is included in the Federal Aviation Administration (FAA) National Plan of Integrated Airport Systems for 2021–2025, in which it is categorized as a local general aviation facility.

Facilities and aircraft 
Sparta/Fort McCoy Airport covers an area of 640 acres (259 ha) at an elevation of 838 feet (255 m) above mean sea level. It has two runways with asphalt surfaces: 11/29 is 4,697 by 100 feet (1,432 x 30 m) and 1/19 is 3,032 by 95 feet (924 x 29 m). The airport also has two asphalt helipads, on Runway 11/29 and taxiway Charlie, both measuring 100 by 100 feet (30 x 30 m), respectively.

For the 12-month period ending June 22, 2022, the airport had 6,000 aircraft operations, an average of 16 per day: 50% military and 50% general aviation. In June 2023, there were 23 aircraft based at this airport:  22 single-engine and 1 helicopter.

See also 
 List of airports in Wisconsin

References

External links 
  at Wisconsin DOT Airport Directory
 Aerial image as of April 1999 from USGS The National Map
 

Airports in Wisconsin
Buildings and structures in Monroe County, Wisconsin
is a Japanese manga series by Rin Mikimoto. Kiss Me at the Stroke of Midnight was serialized in the monthly  manga magazine Bessatsu Friend from April 13, 2015 to May 13, 2020. A live-action film adaptation of the same name was released on December 6, 2019.

Plot

Hinana Hanazawa, a diligent and hard-working honors student, secretly yearns for a fairytale-like romance. One day, popular actor Kaede Ayase comes to film a movie at her high school, in which she is appearing as an extra. Hinana discovers that Kaede's true personality is different from his princely image, but in spite of this, they both fall in love with each other. However, difficulties arise in their relationship due to their different social statuses.

Characters

; portrayed by: Kanna Hashimoto (film)

; portrayed by: Ryota Katayose (film)

Portrayed by: Gordon Maeda (film)

Portrayed by: Alissa Yagi (film)

Media

Manga

Kiss Me at the Stroke of Midnight is written and illustrated by Rin Mikimoto. It was serialized in the monthly magazine Bessatsu Friend from the May 2015 issue released on April 13, 2015 to the June 2020 issue released on May 13, 2020. The chapters were later released in 12 bound volumes by Kodansha under the Kodansha Comics Bessatsu Friend imprint.

At New York Comic Con 2016, Kodansha USA announced that they were publishing the series in English for North American distribution.

Novels

A novel adaptation written by Yui Tokiumi and published by Kodansha under the Kodansha KK Bunko imprint, with illustrations provided by Mikimoto.

Film

In April 2019, the May 2019 issue of Bessatsu Friend announced that a live-action film adaptation was green-lit, with a release date of December 6, 2019 announced later in the year. The film is directed by Takehiko Shinjō and written by Haruka Ōkita, with a starring cast of Generations from Exile Tribe member Ryota Katayose as Kaede and Kanna Hashimoto as Hinana. Additional cast members include Gordon Maeda, Alissa Yagi, Kenichi Endo, Sae Okazaki, Katsuhiro Suzuki, and Wakana Sakai. The film's theme song is "One in a Million (Kiseki no Yoru ni)" performed by Generations from Exile Tribe. A tie-in commercial for Coca-Cola starring Katayose and Ayaka Wilson was produced for and featured in the film.

The film ranked #3 at box office during its opening weekend in 255 theaters nationwide in Japan, selling 159,000 tickets and earning approximately  on its first day. On its second day, it sold an additional 121,000 tickets and earned approximately . The film earned a cumulative total of .

Reception

Rebecca Silverman from Anime News Network complimented the artwork and the juxtaposition of the Cinderella fairytale but cited issues with some of the romance tropes used.

Kiss Me at the Stroke of Midnight was nominated for Best Shōjo Manga at the 43rd Annual Kodansha Manga Awards.

References

External links
 Official movie website 
 

Live-action films based on manga
Manga adapted into films
Shōjo manga
Kodansha manga
Japanese romantic drama films
The Stony Brook Seawolves men's basketball statistical leaders are individual statistical leaders of the Stony Brook Seawolves men's basketball program in various categories, including points, assists, blocks, rebounds, and steals. Within those areas, the lists identify single-game, single-season, and career leaders. The Seawolves represent Stony Brook University in the NCAA Division I Colonial Athletic Association.

Stony Brook played its first Division I season in 1999. These lists are updated through the end of the 2021–22 season.

Scoring

Rebounds

Assists

Steals

Blocks

References

Lists of college basketball statistical leaders by team
Statistical
Tsukrovariv () is a former settlement and part of the selo of Lypniazhka in Novoukrainka Raion of Kirovohrad Oblast. It belongs to Dobrovelychkivka settlement hromada, one of the hromadas of Ukraine. The town, located close to the urban-type settlement of Dobrovelychkivka, was established for the workers of a giant sugar refinery that was once on the site ( means sugar plant in Ukrainian). Nowadays the place is abandoned and mostly a ghost town, keeping just some tens of residents.

Until 18 July 2020, Tsukrovariv and Lypniazhka belonged to Dobrovelychkivka Raion. The raion was abolished in July 2020 as part of the administrative reform of Ukraine, which reduced the number of raions of Kirovohrad Oblast to four. The area of Dobrovelychkivka Raion was merged into Novoukrainka Raion.

References

External links
zpravy.aktualne.cz

Ghost towns in Ukraine
Villages in Novoukrainka Raion
Aleksey Nikolayevich Kamanin (Алексей Николаевич Каманин, born 6 June 1978 in Odessa) is a Russian handball player. He played for the Russia men's national handball team as a right back. He was a part of the team at the 2008 Summer Olympics. On club level he played for Chekhovskiye Medvedi in Russia.

References

1978 births
Living people
Russian male handball players
Handball players at the 2008 Summer Olympics
Olympic handball players for Russia
Sportspeople from Odesa
Nicholas Valentine Maher (died 18 October 1851) was an Irish Repeal Association politician.

He was the son of Thomas Maher, a medical practitioner, and his wife Margaret. In 1845, he married Margaret Jane Herbert, the daughter of Walter Otway Herbert and Mary Miles.

Maher was first elected Repeal Association MP for  at a by-election in 1844—caused by the death of his cousin, Valentine Maher—and held the seat until his own death in 1851.

He was also a member of the Reform Club.

References

External links
 

UK MPs 1841–1847
UK MPs 1847–1852
Members of the Parliament of the United Kingdom for County Tipperary constituencies (1801–1922)
Irish Repeal Association MPs
1851 deaths
The 1942 Nevada Wolf Pack football team was an American football team that represented the University of Nevada as an independent during the 1942 college football season. In their fourth season under head coach Jim Aiken, the team compiled a 4–3–1 record.

Marion Motley, who was later inducted into the Pro Football Hall of Fame, played for the Wolf Pack from 1941 to 1943.

Nevada was ranked at No. 155 (out of 590 college and military teams) in the final rankings under the Litkenhous Difference by Score System for 1942.

Schedule

References

Nevada
Nevada Wolf Pack football seasons
Nevada Wolf Pack football
Alasea is a genus of moths in the family Choreutidae, containing only one species, Alasea corniculata, which is known from Costa Rica.

The length of the forewings is 4.5–5.2 mm for males and 5.2–5.7 mm for females.

Etymology
The species is named for the horn-shaped projection on the valva. The word is derived from the Latin adjective corniculatus.

References

External links
Choreutidae.lifedesks.org:  Holotype picture

Choreutidae
Monotypic moth genera
Moths of Central America
Quinisocaine (INN) or dimethisoquin (BAN and USAN) is a topical anesthetic used as an antipruritic.

Synthesis

The Henry reaction between phthalaldehydic acid (2-Formylbenzoic acid) [119-67-5] (1) and 1-nitropentane [628-05-7] occurs by a mechanism that involves a hydroxy acid (2). Expulsion of water then gives (3). Reduction of the nitro group via catalytic hydrogenation leads to the amine, CID:158569430 (4). Treatment of that amine with sodium hydroxide leads to ring opening of the lactone ring to the intermediary amino acid (5). This cyclises spontaneously to the lactam so that the product isolated from the reaction mixture is in fact the isoquinoline derivative, CID:154188092 (7). Dehydration by means of strong acid gives 3-Butylisocarbostyril [132-90-1] (8). Phosphorus oxychloride converts the oxygen function to the corresponding chloride via the enol forms 3-butyl-1-chloroisoquinoline [87-06-9] (9). Displacement of halogen with the sodium salt from 2-dimethylaminoethanol (10) affords dimethisoquin (11).

References

Local anesthetics
Isoquinolines
Phenol ethers
Dimethylamino compounds
The National Library of Papua New Guinea is the national library of Papua New Guinea. It was founded in 1978 and is located in Port Moresby.

It opened three years after the country's independence from Australia, the Library was established by Australia as a "gift to the government and people of Papua New Guinea" in order to "assist in establishing a collection of items of national significance".

It houses approximately 56,000 items, "including books, films and video footage as well as maps, charts, photographs and microfilm".

References 

Papua New Guinea
Libraries in Papua New Guinea
Buildings and structures in Port Moresby
1978 establishments in Papua New Guinea
Research libraries
Libraries established in 1978
is a national park comprising two separate areas of Aomori, Iwate, and Akita Prefectures, Japan. The Towada-Hakkōda area encompasses Lake Towada, Mount Hakkōda, and most of the Oirase River valley. The Hachimantai area includes Mount Hachimantai, Mount Iwate, Tamagawa Onsen, and . The two areas are  apart, and cover .

Related municipalities
 Aomori: Aomori, Hirakawa, Kuroishi, Towada
 Iwate: Hachimantai, Shizukuishi, Takizawa
 Akita: Kazuno, Kosaka, Semboku

See also

List of national parks of Japan

References

External links
  
  Towada-Hachimantai National Park (JNTO)
  Towada-Hachimantai National Park
 Map of Towada-Hachimantai National Park (area 1)
 Map of Towada-Hachimantai National Park (area 2)

National parks of Japan
Parks and gardens in Akita Prefecture
Parks and gardens in Aomori Prefecture
Parks and gardens in Iwate Prefecture
Protected areas established in 1936
1936 establishments in Japan
George Brown Key (11 February 1882 – November 1958) was a Scottish international footballer who played in both the Scottish and English football leagues.

Career
Key was born in the Dennistoun district of Glasgow, and played at Junior level for Parkhead before stepping up to Heart of Midlothian in 1899. With Hearts, Key won the Scottish Cup in 1901 and the following season, was capped for Scotland against Ireland, also appearing in the away leg of the 'World Championship' against Tottenham Hotspur. He was a Scottish Cup runner-up in 1903.

After a disagreement with Hearts over financial matters, Key was signed by Chelsea in 1905 for their first season in English football. The West London club's manager Jacky Robertson had been an international teammate of Key's three years earlier. Key was only a regular in his first year at Chelsea, and left in 1909.

His brother William was also a Scotland international.

References

External links
George Key Scotland profile at London Hearts Supporters Club 
George Key at 11v11.com

1882 births
1958 deaths
Footballers from Glasgow
Men's association football wing halves
Scottish men's footballers
Parkhead F.C. players
Heart of Midlothian F.C. players
Chelsea F.C. players
Scotland men's international footballers
English Football League players
Scottish Football League players
Date of death missing
Place of death missing
Scottish Junior Football Association players
People from Dennistoun
Scotland men's junior international footballers
The Renegade Film Festival is an annual event held in the Atlanta, Georgia area that specializes in genre film and showcases diverse and independent cinema from marginalized voices. The festival spotlights short and feature films that are typically independently produced and would not otherwise receive theatrical release. In addition to film screenings, the festival hosts panel discussions, workshops, and networking events designed to support and empower independent filmmakers.

The festival has also appeared in Adrian Tofei's top 100 genre festivals list and the MovieMaker top 50 genre festivals list.

History 
The Renegade Film Festival was formerly known as the Women in Horror Film Festival, which was founded in 2017 by Vanessa Ionta Wright and Samantha Kolesnik. The festival was originally dedicated to specifically celebrating and supporting female filmmakers and writers in the horror genre. In 2021, the festival rebranded as the Renegade Film Festival in order to expand its scope and mission to include all marginalized voices and genres.

The Renegade Film Festival's mission is to promote inclusion and visibility in independent cinema, and to bring balance and equality to the film industry. The festival welcomes films made by filmmakers from diverse backgrounds, including women, people of color, members of the LGBTQ+ community, and those with disabilities. It seeks to provide a space for these filmmakers to showcase their work, connect with other filmmakers, and engage with audiences.

The festival has attracted many notable guests and judges, including: Heather Langenkamp, Robert Kurtzman, Lynn Lowry, Trina Parks, Marianne Maddalena, Adam Marcus, Amanda Wyss, Sean Gunn, and Andre Gower.

The festival has been held in different locations in the Atlanta area, with a recent focus on Marietta.

Awards 
The Renegade Film Festival gives out awards called Lizzies in a number of categories for short films, feature films, and unproduced screenplays.

Notable Films 
Some notable films that played the festival include:

2023: Give Me An A, The Once and Future Smash, Retro Freaks, Stag, End Zone 2

2022: Freaky, Take Back the Night, Maya, Souvenirs, The Winter Hunger

2020: Black Lake, The Dark Red, Cold Wind Blowing, Mass Hysteria

2018: Echoes of Fear, Bugs: A Trilogy

2017:  New Nightmare, Eye For an Eye, Rave Party Massacre, Buzzard Hollow Beef, Ruin Me

References

External links 
 Renegade Film Festival website

Underground film festivals
Experimental film festivals
Fantasy and horror film festivals in the United States
LGBT film festivals in the United States
Film festivals in Georgia (U.S. state)