Samantha912/NLP_A2_WTH huggingface dataset:
LifeAct is a 17 amino acid recombinant peptide that stains filamentous actin (F-actin) structures of eukaryotic living or fixed cells. The peptide is a registered trademark of ibidi GmbH. There are several types and combinations of LifeAct that can be utilized depending on the cell type, protocol, and purpose of the analysis.

Lifeact amino acid sequence 
Lifeact 17 amino acid sequence is MGVADLIKKFESISKKE.

Types 

 LifeAct Plasmid
 LifeAct mRNA
 LifeAct Adenovirus
 LifeAct Lentivirus
 LifeAct Protein

Chemistry 
LifeAct-TagGFP2 being the most widely used fluorescent variant compared to other LifeAct constructs is composed of the first 17 amino acid from the Saccharomyces cerevisiae Abp140, an actin-binding protein. The Abp140 is highly conserved among Saccharomyces cerevisiae and other closely related organisms. The 17 amino acid fragment of Abp140 was genetically fused to GFP and fluoresces green when it binds the F-actin structures of living and fixed cells, allowing for visualization of cell mechanics under microscopes.  Previous experiments involving the analysis of cell mechanics had depended on fluorescently labeled phalloidin and actin GFP fusion proteins obtained from utrophin in Xenopus laevis and ABP120 in Dictyostelium discoideum. However, due to their large protein size, markers such as phalloidin and GFP fusion proteins are limited to cells that can be transfected and tend to compete with their orthologous protein. These localization markers affect cellular mechanical properties and F-actin structures, thus making these markers unreliable. An alternative to these markers is Life Act-TagGFP2, which is a much smaller protein and does not affect cell mechanics. Cells synthesize LifeAct-TagGFP2 in a short period of time at a cost-effective making it suitable as an in vivo marker.

Applications in biomedical research 
LifeAct peptides have been used as a universal marker for F-actin visualization in biomedical research. An experiment conducted by Sawant et al. utilized LifeAct GFP to visualize the migration of control border cells in the ovaries of Drosophila  flies, in order to determine how cells move in terms of small and large collectives during development and cancer. Lifeact labels F-actin in border cells and adjacent follicle cells allowed for the detailed examination of border cell membranes and protrusions. Studies regarding the degradation of actin cytoskeleton due to aging relied on LifeAct for the analysis of cytoskeletal organization as a function of age. Transgenic lines that expressed the LifeAct in various tissues of C. elegans were primarily used for imaging.

References 

Protein imaging
Outer Harbour or Harbor may refer to:

 Great Yarmouth Outer Harbour
 Outer Harbor, South Australia
 Outer Harbor railway line
 Outer Harbor railway station
 Outer Harbour Ferry Terminal
 Outer Harbour of Fremantle Harbour
 Toronto Outer Harbour
Sachtler is a manufacturer of film and video camera support products and reporter lighting equipment. The company was founded in 1958 and was bought by the United Kingdom's Vitec Group (now Videndum) in 1995.

History
Wendelin Sachtler was a cinematographer, actor and inventor, living in South-Germany. In 1958 he designed the first tripod head. This so-called gyroscopic head provided not only pans and tilt motion, but also added gyroscopic damping to smooth out camera moves. The new tripod head proved so popular with fellow camera operators  that it led to the foundation of a new company, Sachtler, to meet demand. He worked out of a small workshop in Munich-Schwabing, Germany. After a pair of moves, since 2004 Sachtler GmbH has been located in Eching near Munich. Sachtler has 150 employees worldwide and is represented by retailers in more than 140 countries. The company has production facilities in Germany, Costa Rica and Great Britain.

In 1992 the company was awarded the "Scientific and Engineering Award", an award set up by the Academy of Motion Picture Arts and Sciences.

In 1995 Sachtler was acquired by the Vitec Group PLC.

In 2001 Sachtler and Curt O. Schaller launched the artemis camera stabilizer system at the NAB Show in Las Vegas. The artemis systems were the first modular camera stabilizer systems in the world. In addition, the artemis HD systems were the first Full HD camera stabilizer systems worldwide.

The artemis Trinity system, developed by Curt O. Schaller together with Roman Foltyn in 2015, is the first camera stabilizer system in the world that combines a mechanical stabilization system with an electronic one.

In April 2016, ARRI acquired the artemis camera stabilizer systems developed by Curt O. Schaller from Sachtler / Vitec Videocom.

Products

The company's portfolio can be divided into: 
 Camera support
 High quality bags for storage and transportation of professional broadcast, camera and audio equipment

The camera support division includes the production of Sachtler fluid heads, tripods and pedestals and professional bags for cameramen and sound operators.

References

External links
Sachtler
Vitec Group

Photography companies of Germany
Companies based in Upper Bavaria
German brands
is a sub-kilometer asteroid, classified as near-Earth object of the Aten group, that is a temporary horseshoe companion to the Earth. It is the 11th known Earth horseshoe librator. Prior to a close encounter with the Earth on 15 December 2015,  was an Apollo asteroid.

Discovery 

 was discovered on 16 December 2015 by G. J. Leonard and R. G. Matheny observing
for the Catalina Sky Survey. As of 9 March 2016, it has been observed 47 times with an observation arc of 5 days.

Orbit and orbital evolution 

 is currently an Aten asteroid (Earth-crossing but with a period less than a year). Its semi-major axis (currently 0.99753 AU) is similar to that of Earth (1.00074 AU), but it has a moderate eccentricity (0.2791) and very low orbital inclination (1.6249°). It alternates between being an Aten asteroid and being an Apollo asteroid, although its orbital evolution is rather chaotic. As of 9 March 2016, this object is the 16th known Earth co-orbital and the 11th known object following a horseshoe path with respect to our planet. Asteroid  follows an asymmetrical horseshoe path with respect to our planet; the value of its relative mean longitude oscillates about 180°, but enclosing 0°.

Physical properties 

With an absolute magnitude of 27.4 mag, it has a diameter in the range 9–22 meters (for an assumed albedo range of 0.04–0.20, respectively).

See also 

 54509 YORP
 
 3753 Cruithne

Notes 

  This is assuming an albedo of 0.20–0.04.

References 

Further reading
 Understanding the Distribution of Near-Earth Asteroids Bottke, W. F., Jedicke, R., Morbidelli, A., Petit, J.-M., Gladman, B. 2000, Science, Vol. 288, Issue 5474, pp. 2190–2194.
 A Numerical Survey of Transient Co-orbitals of the Terrestrial Planets Christou, A. A. 2000, Icarus, Vol. 144, Issue 1, pp. 1–20.
 Debiased Orbital and Absolute Magnitude Distribution of the Near-Earth Objects Bottke, W. F., Morbidelli, A., Jedicke, R., Petit, J.-M., Levison, H. F., Michel, P., Metcalfe, T. S. 2002, Icarus, Vol. 156, Issue 2, pp. 399–433.
 Transient co-orbital asteroids Brasser, R., Innanen, K. A., Connors, M., Veillet, C., Wiegert, P., Mikkola, S., Chodas, P. W. 2004, Icarus, Vol. 171, Issue 1, pp. 102–109.
 A trio of horseshoes: past, present and future dynamical evolution of Earth co-orbital asteroids 2015 XX169, 2015 YA and 2015 YQ1 de la Fuente Marcos, C., de la Fuente Marcos, R. 2016, Astrophysics and Space Science, Vol. 361, Issue 4, article 121 (13 pp).

External links 
 Discovery MPEC 
  data at MPC
 ADS MPEC record
 
 
 

Minor planet object articles (unnumbered)
Earth-crossing asteroids

20151216
Anthony N. "Tony" Lawrence III (born April 3, 1965) is an American judge on the Mississippi Court of Appeals.

References

External links 
 
 

1965 births
21st-century American judges
21st-century American lawyers
American prosecutors
District attorneys in Mississippi
Living people
Mississippi Court of Appeals judges
People from Pascagoula, Mississippi
University of Mississippi School of Law alumni
University of Southern Mississippi alumni
The Asian Cup Winners' Cup  was an association football competition run by the Asian Football Confederation (AFC). The competition was started in 1991 as a tournament for all the domestic cup winners from countries affiliated to the AFC. The winners of the Cup Winners' Cup used to contest the Asian Super Cup against the winners of the Asian Club Championship. The most successful clubs in the competition are Al Hilal from Saudi Arabia and Nissan FC from Japan.

Finals

Records and statistics

The following table lists countries by number of winners and runner-up in Asian Cup Winners' Cup.

By nation

By club 
The following table lists clubs by number of times winners and runners-up in Asian Cup Winners' Cup.

1 including Nissan FC.  
2 Yokohama Flügels was merged with Yokohama Marinos to Yokohama F. Marinos in 1999.

Winning coaches
The following table lists the winning coaches of the Asian Cup Winners' Cup.

External links
 Asian Cup Winners Cup – RSSSF

 
Defunct Asian Football Confederation club competitions
Recurring sporting events established in 1990
Recurring sporting events disestablished in 2002
Upper Dallachy is a small village situated in Moray, Scotland, approximately 2 miles south-east of Spey Bay.

The village is located next to the now-abandoned RAF Dallachy airfield, which was used frequently in the Second World War and was the site of a number of bombings. It is one of four small settlements situated on the east side of the River Spey.

External links

Villages in Moray
Hund may refer to:
Hund (surname)
Hund (village), a village in Swabi district, Pakistan
Hund School, Kansas, USA
Hund (card game), German card game
 Hund or Voller Hund, German card game
 Hund Şehzade (1422–1455), Ottoman princess

See also
Hundt, a surname

Surnames from nicknames
The protected areas of the Sierra Nevada, a major mountain range located in the U.S. states of California and Nevada, are numerous and highly diverse. Like the mountain range itself, these areas span hundreds of miles along the length of the range, and over 14,000 feet of elevation from the lowest foothills to the summit of Mount Whitney.

The Sierra Nevada, as a natural region, does not have exactly defined borders. In particular, to the north, there is a wide transition zone where the Cascade Range and the Sierra Nevada meet. As a result, it is difficult to delineate a border between these two mountain ranges. This list uses the common definition of the Sierra Nevada as the mountain range extending from Fredonyer Pass in the north to Tehachapi Pass in the south. It is also difficult to delineate between the Sierra Nevada mountains, the range's foothills, and the Central Valley; this article also considers any elevated and hilly terrain east of the flat Central Valley to be part of the range. Areas outside those bounds may also be included if they are widely associated with the Sierra Nevada.

National parks and monuments

National forests

Wilderness areas

Other federal protected areas
 Blue Ridge National Wildlife Refuge
 Jawbone–Butterbredt Area of Critical Environmental Concern
 The Red Hills of Tuolumne County Area of Critical Environmental Concern

State parks
 Burton Creek State Park
 Calaveras Big Trees State Park
 D. L. Bliss State Park
 Donner Memorial State Park
 Ed Z'berg Sugar Pine Point State Park
 Emerald Bay State Park
 Grover Hot Springs State Park
 Lake Tahoe–Nevada State Park (Nevada)
 Plumas-Eureka State Park
 South Yuba River State Park
 Van Sickle Bi-State Park (co-managed by California and Nevada)
 Washoe Meadows State Park

State recreation areas
 Auburn State Recreation Area
 Folsom Lake State Recreation Area
 Kings Beach State Recreation Area
 Lake Oroville State Recreation Area
 Lake Valley State Recreation Area
 Millerton Lake State Recreation Area
 Tahoe State Recreation Area

State forests
 Mount Zion Demonstration State Forest
 Mountain Home Demonstration State Forest

State historic parks
 Columbia State Historic Park
 Empire Mine State Historic Park
 Indian Grinding Rock State Historic Park
 Malakoff Diggins State Historic Park
 Marshall Gold Discovery State Historic Park
 Railtown 1897 State Historic Park
 Wassama Round House State Historic Park

See also
 Protected areas of California
 Protected areas of the United States

Notes

References

Sierra Nevada
Sierra Nevada
Sierra Nevada (United States)-related lists
Sierra Nevada
Fear No ART Presents The Dinner Party is a live arts talk show that features host Elysabeth Alfano sitting down with three individuals from differing creative fields for a conversation over a dinner prepared by a guest chef. It premiered in January 2012. The show is also streamed live online and distributed through TiVo.

Concept 
The concept for the show originated in a dinner party that Alfano held at her home for several friends from the creative community in Chicago. That dinner resulted in lively conversations, and The Dinner Party was created as an attempt to replicate that experience with a public audience. Alfano specifically chooses guests from differing creative fields to discuss the commonalities between their work. The show's tagline is "The best conversations happen over dinner." Alfano is the creator, executive producer, and host of the show.

The audience is encouraged to participate in these conversations during the show. Both the live audience and those watching the internet broadcast of show are allowed to use Twitter to ask questions of the guests. Audience members who have their questions "picked" win prizes.

Guests in attendance at the live show are also share in the culinary experience; the audience is served the same appetizer and entrée as Alfano and her guests, in addition to wine samplings and Vosges chocolate.

Previous guests have included international violinist Rachel Barton Pine, rapper Dessa, Sound Opinions’ Jim DeRogatis, NPR’s Peter Sagal, Saturday Night Live alums Tim Kazurinsky and Nora Dunn, Project Runway’s Peach Carr, James Beard chef Paul Kahan, Michelin star chef Homaro Cantu, award winning director Steve James, international spoken word artist Kevin Coval, Goodman Theatre’s Robert Falls, Sex and the City and Chicago Fire TV star David Eigenburg, author Scott Turow, film critic Richard Roeper, Chicago Bears tight end Martellus Bennett and Chicago Bears general manager Phil Emery, among many others.

Show Format 
Each show begins with an opening act. The main show runs for 90 minutes, with host Elysabeth Alfano facilitating conversation between her three guests. Alfano picks guests from a variety of fields to talk about the creative experience. Each guest performs live or has their work featured in some way throughout the course of the show. Additionally, demo videos highlight the food that the chef prepares for the guests and the audience.

Venue 
The show premiered January 30, 2012 at the Mayne Stage in Chicago's Rogers Park neighborhood. The lineup featured artist Tony Fitzpatrick, artist and The Mekon’s band member Jon Langford, and director Rachel Rockford as guests, with Chef Homaro Cantu of MOTO.

On September 20, 2012, the show was held at Navy Pier as part of the inaugural EXPO Chicago Art Exhibition.

As of April 1, 2013, the show is held at City Winery Chicago in Chicago's West Loop neighborhood.

References 

American non-fiction web series
Japanese art is collected by museums, galleries and private collectors in many countries around the world.

See also 
 List of museums of Asian art
 List of museums

References 

Collections
Japanese art
Sir William Knighton (1776–1836) was Private Secretary to the Sovereign, George IV.

William Knighton may also refer to:

William C. Knighton (1864–1938), American architect
Sir William Wellesley Knighton, 2nd Baronet (1811–1885) of the Knighton baronets
William Myles Knighton (born 1931), British civil servant
Hayatabad may refer to:

 Hayatabad township is a suburb of Peshawar, Pakistan
 Hayatabad village or hamlet, sometimes also called 'Kot Hayat', near Sheikhupura, Pakistan, named after Nawab Muhammad Hayat Khan, CSI.

Hayatabad () may also refer to:

 Hayatabad-e Khalifeh, Iran
 Hayatabad-e Majidi, Iran
 Heyatabad (disambiguation)
The following lists events that happened during 2014 in Poland.

Incumbents

Elections

Events

May
25 May 
2014 European Parliament election in Poland
2014 Kraków referendum

June
14 June - Beginning of Polish bugging affair (pl)

August
19 August - The second Polish scientific satellite, Heweliusz, is launched.

September
15 September - Ewa Kopacz becomes Poland's second female prime minister.

December
10 December - The Constitutional Court overturns the 2013 ban on ritual slaughter of animals.

Deaths
10 January - Zbigniew Messner, Communist politician (born 1929)
25 May - Wojciech Jaruzelski, former Communist leader of Poland (born 1923)
22 June - Grzegorz Knapp, motorcycle racer (born 1979)
24 December - Krzysztof Krauze, film director (born 1953)
26 December - Stanisław Barańczak, poet (born 1946)
30 December - Marian Jurczyk, Solidarność activist (born 1935)

See also 
 2014 in Polish television

References 

 
2010s in Poland
Poland
Poland
Years of the 21st century in Poland
Wilbertomorphidae is a family of karyorelictean ciliates. The family is monotypic, because it contains a single genus Wilbertomorpha with a single known species, Wilbertomorpha colpoda.

Like most karyorelicteans, Wilbertomorpha lives in the marine interstitial habitat, between sediment grains. The cells are oval-shaped and about 45 µm long by 15 µm wide. Its distinctive features are a reduced oral apparatus ("mouth") and colorless granules of different shapes (rod-, cross-, and oval-shaped) in the cortex of the cell. Its phylogenetic relationship to other families of karyorelictean ciliates is unclear, because its morphology (ciliature) most closely resembles the order Loxodida, whereas molecular phylogenetics places it closer to the family Geleiidae. It was first collected from a beach at Qingdao, China. The name Wilbertomorpha was coined in honor of the German ciliatologist Norbert Wilbert.

References

Karyorelictea
Ciliate families
Monogeneric eukaryote families
Monotypic SAR supergroup taxa
Micrurus filiformis, the thread coral snake or slender coral snake, is a species of snake of the family Elapidae.

The snake is found in Colombia, Brazil, and Peru.

References 

filiformis
Reptiles described in 1859
Taxa named by Albert Günther 
Reptiles of Brazil
Reptiles of Colombia
Reptiles of Peru
The 1967 All-Ireland Under-21 Hurling Championship was the fourth staging of the All-Ireland Under-21 Hurling Championship. The championship began on 14 May and ended on 3 September 1967.

Tipperary won the title after defeating Dublin by 1-8 to 1-7 in the final.

Teams

Team summaries

Results

Leinster Under-21 Hurling Championship

Munster Under-21 Hurling Championship

First round

Semi-finals

Final

Ulster Under-21 Hurling Championship

All-Ireland Under-21 Hurling Championship

External links
 1967 All-Ireland Under-21 Hurling Championship results

Under-21
All-Ireland Under-21 Hurling Championship
The 1999 Rivers State gubernatorial election occurred in Nigeria on January 9, 1999. The PDP nominee Peter Odili won the election, defeating the APP candidate.

Peter Odili emerged PDP candidate.

Electoral system
The governor of Tivers State is elected using the plurality voting system.

Primary election

PDP primary
The PDP primary election was won by Peter Odili.

Results
The total number of registered voters in the state was 2,207,000. Total number of votes cast was 747,296, while number of valid votes was 1,580,807. Rejected votes were 7,521.

References 

Rivers State gubernatorial elections
Rivers State
Rivers State gubernatorial election
Euxoa basigramma is a moth of the family Noctuidae. It is found in Turkey, Iran and from Ukraine, Moldova, northern, north-western, central, eastern and south-western Russia to Mongolia.

External links
Fauna Europaea

Euxoa
Insects of Turkey
Moths described in 1870
Thierry Daubresse is a Paralympian athlete from Belgium competing mainly in category F42 shot put events.

Thierry competed in the 1996 Summer Paralympics in  Atlanta where he won a gold medal in the F42 shot put as well as competing in the pentathlon, discus and javelin.  He failed in his attempt to defend his title in 2000 when finishing third in the shot put as well as competing in the discus.

External links
 profile on paralympic.org

Paralympic athletes for Belgium
Athletes (track and field) at the 1996 Summer Paralympics
Athletes (track and field) at the 2000 Summer Paralympics
Paralympic gold medalists for Belgium
Paralympic bronze medalists for Belgium
Living people
Medalists at the 1996 Summer Paralympics
Medalists at the 2000 Summer Paralympics
Year of birth missing (living people)
Paralympic medalists in athletics (track and field)
Belgian male shot putters
Shot putters with limb difference
Paralympic shot putters
Zichem is a village of the town of Scherpenheuvel-Zichem in the Belgian province of Flemish Brabant. Zichem was an independent municipality until the municipal redistribution of 1977. Zichem is on the list of The most beautiful villages in Flanders as the most beautiful village of Flemish Brabant.

History 
Zichem belonged to Maria van Loon-Heinsberg, who was a descendant of the Counts of Loon. After her marriage in 1440 to Jan IV of Nassau, Zichem became part of the county of Nassau-Dillenburg.

Before the outbreak of the Eighty Years' War, Zichem was a thriving town. During the siege of Zichem in 1578 by Alexander Farnese, almost the entire garrison was killed. In 1580, Zichem was hit by an earthquake which toppled the castle's keep. On October 8, the Staatsen retook Zichem. In 1599, a large ignited city fire put a permanent end to the town, and the town was destroyed by the fire.

Geography 
The Demer(river) that flows through the city forms the border between the Kempen region in the north and the Hageland region in the south.

Sights to see 

 the Market Square, a copy of the village squares in Provence
 The 
 The 
 The birthplace of Ernest Claes
 the Witpeerd - a toll house built in 1617 to collect tolls on shipping traffic on the Demer.

Zichem and Ernest Claes 
Zichem is the birthplace of Ernest Claes, one of Flanders' most widely read writers in the second half of the 20th century.His birthplace is now a museum.

Zichem is also very well known for the book De Witte van Ernest Claes, which was later filmed several times.

Television Series 
The television series Wij, Heren van Zichem was based on several conjoined stories by Ernest Claes. Some of the filming for the series took place in the city.

Two films were also made "De Witte" (1934) by Jan Vanderheyden with a.o. Jef Bruyninckx, and "De Witte van Sichem" by Robbe De Hert (1980).

born in Zichem 

Ernest Claes (1885-1968), writer

Peter Jan Beckx(1795-1887), superior of the Jesuits.

Johnny White (1946-2014), singer

References

Former municipalities of Flemish Brabant
AIETOH is a 1995 EP by Japanese group Super Junky Monkey. It showcased their fusion of rap, hardcore, punk, and funk music. It was released only in Japan.

Track listing
"A•I•E•T•O•H"
"Fuck That Noise"
"Time Is Culture"
"Blah, Blah, Blah"

Personnel 

 Mutsumi ‘623’ Fukuhara – vocals
 Keiko – guitar
 Shinobu Kawai – bass
 Matsudaaahh – drums

References

1995 EPs
Super Junky Monkey albums