Samantha912/NLP_A2_WTH huggingface dataset:
TCEP (tris(2-carboxyethyl)phosphine) is a reducing agent frequently used in biochemistry and molecular biology applications.  It is often prepared and used as a hydrochloride salt (TCEP-HCl) with a molecular weight of 286.65 gram/mol. It is soluble in water and available as a stabilized solution at neutral pH and immobilized onto an agarose support to facilitate removal of the reducing agent.

Applications
TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis.

Compared to the other two most common agents used for this purpose (dithiothreitol and β-mercaptoethanol), TCEP has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent (in the sense that TCEP does not regenerate—the end product of TCEP-mediated disulfide cleavage is in fact two free thiols/cysteines), more hydrophilic, and more resistant to oxidation in air.  It also does not reduce metals used in immobilized metal affinity chromatography.

TCEP is particularly useful when labeling cysteine residues with maleimides. TCEP can keep the cysteines from forming di-sulfide bonds and, unlike dithiothreitol and β-mercaptoethanol, it will not react as readily with the maleimide. However, TCEP has been reported to react with maleimide under certain conditions.

TCEP is also used in the tissue homogenization process for RNA isolation.

For Ultraviolet–visible spectroscopy applications, TCEP is useful when it is important to avoid interfering absorbance from 250 to 285 nanometers which can occur with dithiothreitol. Dithiothreitol will slowly over time absorb more and more light in this spectrum as various redox reactions occur.

History
Reduction of biomolecules with trialkyphosphines received little attention for decades because historically available phosphines were extremely malodorous and/or insoluble in water. In 1969, TCEP was reported as an oderless and water-soluble trialkyphosphine suitable for biochemical use, however the potential use of TCEP for biochemical applications was almost totally ignored for decades. In 1991, Burns reported a new convinient synthetic procedure for TCEP, which set off TCEP becoming more widely available and marketed as a "new" reducing agent for biochemical use, & thus TCEP came into more widespread use throughout the 1990s.

Reactions

Some other side reactions have also been reported:
 Conversion of a cysteine residue into alanine in the presence of TCEP and heat (90˚C).
 Slow (but significant, 40% cleavage reported for two week storage at 4˚C) protein backbone cleavage at cysteine residues under mild conditions.

Use in biological research
TCEP is available from various chemical suppliers as the hydrochloride salt. When dissolved in water, TCEP-HCl is acidic. A reported preparation is a 0.5 M TCEP-HCl aqueous stock solution that is pH adjusted to near-neutral pH and stored frozen at -20˚C. TCEP is reportedly less stable in phosphate buffers.

See also
 2-Mercaptoethanol (BME)
 Dithiothreitol (DTT)
 Dithiobutylamine (DTBA)

References

Carboxylic acids
Tertiary phosphines
Reducing agents
Marcus Fraser may refer to:

 Marcus Fraser (golfer) (born 1978), Australian golfer
 Marcus Fraser (footballer) (born 1994), Scottish footballer
History

The Kitchener–Waterloo Braves were founded in 1967. 2017 marked their 50th anniversary of continuous operation. In 2020 the lacrosse team now is recognized as the KW Jr A Lacrosse CLub

Season-by-season results
Note: GP = Games played, W = Wins, L = Losses, T = Ties, Pts = Points, GF = Goals for, GA = Goals against

External links
Braves Webpage
The Bible of Lacrosse
Unofficial OLA Page

Ontario Lacrosse Association teams
Sport in Kitchener, Ontario
The 1966 Australian Grand Prix was a motor race staged on 20 February 1966 at the Lakeside Circuit in Queensland, Australia. The race, which had 15 starters, was open to Racing Cars complying with the Australian National Formula or the Australian 1½ Litre Formula. It was both the 31st Australian Grand Prix and race 6 of the 1966 Tasman Championship for Drivers.

Graham Hill won the race driving BRM P261. It was his only Australian Grand Prix victory.

Classification 

Results as follows:

Notes 
Pole position: Jackie Stewart – 0'55.5
Fastest lap: Jackie Stewart / Graham Hill – 0'55.9

References

Grand Prix
Australian Grand Prix
Tasman Series
Sport in Brisbane
Australian Grand Prix
Tillandsia lotteae is a species of flowering plant from the genus Tillandsia. This species is endemic to Bolivia.

References

lotteae
Flora of Bolivia
Parachela hypophthalmus is a species of cyprinid in the genus Parachela. It inhabits Thailand, Malaysia and Indonesia, in Sumatra and Borneo and has a maximum length of . It has been assessed as least concern by the IUCN, and is considered harmless to humans.

References

Cyprinid fish of Asia
Fish of Thailand
Fish of Malaysia
Fish of Indonesia
IUCN Red List least concern species
Cyprinidae
Wendy Bisaro is a Canadian politician who represented Frame Lake in the Legislative Assembly of the Northwest Territories from 2007 to 2015.

Early life and education
Bisaro was born in Montreal, Quebec, and in 1968 earned a bachelor of education degree from McGill University with a specialty in physical education.

Career
After teaching high school in Montreal, she moved to Yellowknife in 1971 and taught at Sir John Franklin High School. From 1985 to its closure in 2004, she worked at Mack Travel; she then became an adult instructor for Canadian North.

In sport, she played for, coached, and was an organizer in the Yellowknife Basketball Association and the Yellowknife Ladies Softball Association and was a co-founder of Sport North, where she served as vice president from 1984 to 1988. She competed, coached, and was a member of the mission staff for the Canada Games, competed in the Arctic Winter Games and was Northwest Territories Chef de Mission for the 1984 games and a director of the Host Society for the 1988 games and vice president of the Host Society for the 2008 games.

Political career
Bisaro was elected in 1988 to the board of trustees for Yellowknife Education District #1, and served four terms, for five years as board chair, until 2000, when she successfully ran for election to Yellowknife City Council. She was re-elected in 2003 to a second term, during which she was chosen to be deputy mayor.

In the 2007 provincial election she was elected MLA for Frame Lake, succeeding Charles Dent, who did not run for reelection. She defeated two other candidates with 56% of the vote. Voter turnout was low at 45%. In 2011 she won reelection with 55% of the vote against two other candidates. Turnout was 30%. She was elected chairperson of the Standing Committee on Priorities and Planning in May 2013. She declined to run for a third term in 2015.

References

External links
Wendy Bisaro on Facebook

Members of the Legislative Assembly of the Northwest Territories
McGill University Faculty of Education alumni
Women MLAs in the Northwest Territories 
Yellowknife city councillors
Politicians from Montreal
Living people
Women municipal councillors in Canada
Year of birth missing (living people)
21st-century Canadian politicians
21st-century Canadian women politicians
Ekaterina Andreevna Kazionova (; born 13 January 1999) is a Russian tennis player.

Kazionova has career-high WTA rankings of 280 in singles and 310 in doubles. Up to date, she has won two singles titles and nine doubles tournaments on the ITF Circuit.

Kazionova made her WTA Tour main-draw debut at the 2022 Morocco Open, after receiving a wildcard entry into the doubles tournament, partnering with Yasmine Kabbaj.

ITF Circuit finals

Singles: 10 (2 titles, 8 runner-ups)

Doubles: 23 (9 titles, 14 runner-ups)

References

External links
 
 

1991 births
Living people
Russian female tennis players
21st-century Russian women
21st-century Russian people
Gibbet Mill, Tillingham Mill, Barry's Mill or New Mill is a grade II listed cosmetically reconstructed smock mill at Rye, East Sussex, England. Today it serves as bed and breakfast accommodation.

History

A mill has stood on this site since 1596, and a post mill is known to have been built here in 1758. Gibbet Mill was built in 1824, the name Barry's mill coming from an early miller. The mill was working by wind until 1912, and was used as a bakery until 13 June 1930 when it was burnt down. The new mill was erected in 1932, Neve's of Heathfield being responsible for the millwrighting work.

Description

As built, Gibbett Mill was a four-storey smock mill on a single-storey brick base, with a stage at first-floor level. In 1844 she had four Patent sails but latterly was worked with four Spring sails. These were carried in a cast-iron Windshaft. The cap was in the Kentish style, winded by a fantail. The mill drove three pairs of millstones.

The replica mill bears very little resemblance to an actual working windmill. It has a shorter smock, making the mill appear squatter than the original. The cap is exaggerated in height and is not designed to turn into the wind. Replica sails and fantail are carried. The reconstructed stage is wider than the original.

Millers

Frederick Barry: 1824–1844
John Smith: 1848–1879
Gorge Standed: 1882–1899
Webb: 1917

References for above:-

References

External links

Windmill World Page on Gibbet windmill.
Rye Windmill B&B website.

Further reading
 Online version 

Smock mills in England
Grinding mills in the United Kingdom
Windmills completed in 1824
Industrial buildings completed in 1932
Grade II listed buildings in East Sussex
Windmills in East Sussex
Rye, East Sussex
Octagonal buildings in the United Kingdom
Matt Simpson may refer to:

 Matt Simpson (poet) (1936–2009), British poet and literary critic
 Matt Simpson (beer judge), American expert on craft beer and brewing
 Matt Simpson (racing driver) (born 1981), British racing driver
 Matt Simpson (goalball) (born 1990), American goalball player
 Matt Simpson (New York politician), member of the New York State Assembly from the 114th district
 Matt Simpson (Alabama politician), member of the Alabama House of Representatives

See also
 Matthew Simpson (1811-1884), American bishop of the Methodist Episcopal Church
 Matthew Simpson (footballer) (born 1967), Australian rules footballer
The Arnhem land ctenotus (Ctenotus quirinus)  is a species of skink found in Northern Territory in Australia.

References

quirinus
Reptiles described in 2007
Taxa named by Paul Horner (herpetologist)
Skinks of Australia
"Metal on Metal" () is an instrumental by Kraftwerk from their 1977 album Trans-Europe Express. This track, combined with  "Abzug", the track immediately succeeding it (and considered part of "Metal on Metal" on English pressings), forms an extended coda to "Trans-Europe Express".

Sampling controversy 
Producer Moses Pelham sampled two seconds of the song and re-used it in the 1997 song  "Nur mir" performed by German rapper Sabrina Setlur. Pelham lost in court to Ralf Hütter and Florian Schneider of Kraftwerk for copyright infringement, but was acquitted after an appeal.

In a 2016, The Guardian reported that the court found in favor of Setlur. However, in July 2019, the European Court of Justice (ECJ) overturned the appeal, ruling in favour of Kraftwerk.

References 

Kraftwerk songs
Sampling controversies
1977 songs
The Wilmington, Chadbourn and Conway Railroad was a Southeastern railroad that operated between Chadbourn, North Carolina and Conway, South Carolina near the end of the 19th century.

History
The Chadbourn Lumber Company of Chadbourn, North Carolina, built the line to haul timber.  It connected with the Wilmington and Manchester Railroad in Chadbourne.

The line crossed the North Carolina line at Tabor City, North Carolina.  It continued south through Loris, South Carolina into Conway, South Carolina to its terminus at the Waccamaw River.

The line was sold at foreclosure in 1895 and renamed the Wilmington and Conway Railroad. The following year, the Wilmington and Conway was sold to the Wilmington, Columbia and Augusta Railroad (the successor of the Wilmington and Manchester Railroad).

In 1898, the line came under the ownership of the Atlantic Coast Line Railroad.  In 1912, the Atlantic Coast Line bought the Conway Seashore Railroad, which extended from Conway to Myrtle Beach.  After that, the line was known as their Conway and Myrtle Beach Branch.  Both freight and passenger trains to Myrtle Beach ran the line.

The Atlantic Coast Line became the Seaboard Coast Line Railroad in 1967 after merging with their former rival, the Seaboard Air Line Railroad.  The Seaboard Coast Line Railroad designated the line as the Myrtle Beach Subdivision.  In 1980, the Seaboard Coast Line's parent company merged with the Chessie System, creating the CSX Corporation.  The CSX Corporation initially operated the Chessie and Seaboard Systems separately until 1986, when they were merged into CSX Transportation.

Current operation
Today, the line is still in service and it is operated by the R.J. Corman Railroad Group

Stations

References

Defunct South Carolina railroads
Defunct North Carolina railroads
Railway companies established in 1885
Railway companies disestablished in 1895
American companies established in 1885
American companies disestablished in 1895
Livingly Media (formerly Zimbio, Inc.) is an American digital media company based in Redwood City, California that publishes six lifestyle sites for women: Livingly.com, Lonny.com, Mabelandmoxie.com, Itsrosy.com, Zimbio.com, and StyleBistro.com. The six sites publish original articles, high resolution photography, social videos, and games, reaching a combined audience of over 25 million global monthly readers, according to Quantcast. Livingly covers women's lifestyle, Lonny covers home design, Mabel + Moxie covers parenting, It's Rosy covers women 50+, Zimbio covers entertainment news, and StyleBistro covers celebrity fashion and beauty.

The company was founded as Zimbio, Inc. in 2006 by Tony Mamone and Danny Khatib and venture-backed by Menlo Ventures, Great Oaks Capital, and Draper Richards. The name was changed to Livingly Media in August 2012, shortly after acquiring Lonny magazine. The management team consists of CEO Erica Carter, COO Christophe Decker, Chief Content Office Jill Slattery, Chief Product Officer Erik Kokkonen, and Chief Technology Officer Bruce Martin. In 2015, Livingly Media was acquired by the European digital publishing company Aufeminin.com, then a subsidiary of Axel Springer. In 2018, Aufeminin was acquired by TF1 (Télévision Française 1 S.A). Aufeminin is publicly traded by Euronext Paris in compartment B. (MNEMO : FEM).

History
In May 2006, the company launched its first website, Zimbio.com which focused on entertainment news including movies, music, TV and celebrities.

In July 2010, the company launched StyleBistro.com to focus on fashion and beauty – including what celebrities are wearing, trends and products.

In 2012, the company purchased Lonny Magazine. The terms of the deal were not disclosed. It was redesigned and launched as Lonny.com in November 2012. Irene Edwards joined as Executive Editor in March 2013.

In February 2015, the company announced that it was being acquired by the Axel Springer-owned Aufeminin Group.

In July 2015, the company launched Livingly.com, a lifestyle website for women.

In April 2018, Livingly Media's parent company, Aufeminin, was acquired by TF1(Télévision Française 1 S.A).

In May 2018, the company launching two new websites, Mabel + Moxie and It's Rosy.

In 2022, TF1 sold Livingly Media to Recurrent.

Other platforms
All of Livingly's websites have mobile versions.  As of February 2017, Livingly had over 26.1 million monthly readers, of which 9.2 million accessed the sites via mobile devices, according to Quantcast.

Board members
 Marie-Laure Sauty de Chalon, Aufeminin.com
 Christophe Decker, Aufeminin.com
 Erica Carter, CEO

Financing history
Series A, August 2007 – $6.8 Million
Investors:
 Menlo Ventures
 Draper Richards
 Sand Hill Angels 6, LLC

Series B, May 2012 – $8.9 Million
Investors:
 Menlo Ventures
 Great Oaks Venture Capital
 Draper Associates
 Comerica Bank
 Insikt Ventures
 Fenwick & West
 Sand Hill Angels 6, LLC

Offices
 101 Redwood Shores Pkwy Suite 150, Redwood City, CA 94065

References

External links

Published by Livingly Media
 It's Rosy
 Lon NY

Blog hosting services
Ari Pakarinen (born 14 May 1969) is a retired male javelin thrower from Finland. His personal best throw is 85.18 metres, achieved in July 1995 in Lapua.

He finished fifth at the 1993 World Championships. He later qualified for the final at the 2002 European Championships, but finished last with no valid result.

International competitions

Seasonal bests
1986 - 56.04
1987 - 64.04
1988 - 71.46
1989 - 79.39
1990 - 80.76
1991 - 82.52
1992 - 84.00
1993 - 83.08
1994 - 83.38
1995 - 85.18
1996 - 77.64
1997 - 77.80
1998 - 76.70
1999 - 77.97
2000 - 78.71
2001 - 81.27
2002 - 83.03
2003 - 76.80
2004 - 79.27

References

1969 births
Living people
Finnish male javelin throwers
World Athletics Championships athletes for Finland
20th-century Finnish people
21st-century Finnish people
Anu Irene Laamanen (born 30th October 1958) is a Finnish diplomat. She has been employed by the Ministry for Foreign Affairs since 1986.

Since autumn 2022, Laamanen has been the Finnish Ambassador in Iceland. Before that she was in charge of the National Security Authority of the Ministry of Foreign Affairs (NSA) from 2016 to 2022.

Formerly, she has been Finland's permanent UN representative in Vienna and the Finnish Ambassador in Austria for the period 2013–2016, and the Deputy Head of Department and Head of Unit, of the Political Department of the Ministry for Foreign Affairs. In addition to Vienna, she has worked in embassies in Geneva and Oslo and the Permanent Representations of Finland in the OSCE and the UN.

References 

1958 births
Living people
People from Hämeenlinna
Ambassadors of Finland to Austria
Permanent Representatives of Finland to the United Nations
Finnish women diplomats
Finnish women ambassadors
Calvi – Sainte-Catherine Airport (, )  is an airport located 6 km southeast of Calvi, a commune of the Haute-Corse department in France, on the island of Corsica.

Airlines and destinations
The following airlines operate regular scheduled and charter flights at Calvi – Sainte-Catherine Airport:

Statistics

References

External links 
Calvi Airport (official site) 
Aéroport de Calvi (official site) 
Aéroport de Calvi - Sainte-Catherine at Union des Aéroports Français 

Airports in Corsica
Buildings and structures in Haute-Corse
The 1934 United States Senate election in Virginia was held on November 6, 1934. Incumbent Senator Harry F. Byrd, Sr. was re-elected to his first full (and his second overall) term after defeating Republican Lawrence C. Page in a landslide.

Results

References

Virginia
1934
United States Senate
The Huron Subdivision or Huron Sub is a railway line owned and operated by the Rapid City, Pierre and Eastern Railroad (RCPE), a subsidiary of Genesee & Wyoming. The line stretches for  across southwestern Minnesota and southeastern South Dakota, forming the eastern end of the RCPE shortline network. Originally the line was a part of the Chicago and North Western Railway. From 1986 it was a part of Dakota, Minnesota and Eastern Railroad, which later became a subsidiary of Canadian Pacific Railway.

The line is dark territory, meaning that it is not signalled and not equipped with centralized traffic control or automatic block signalling systems. The line is dispatched via radio using track warrant control.

Huron Subdivision connects with Canadian Pacific Tracy Subdivision at Tracy, Minnesota, as well as Pierre Subdivision and Yale Spur Subdivision (a former Great Northern Railway line) at Huron, South Dakota.

RCPE trains may continue to Mankato using trackage rights to interchange with the Union Pacific railroad.

History
The Huron Subdivision is a direct descendant of the railroad line featured in the four De Smet novels within the overall series of children's novels written by Laura Ingalls Wilder known as the Little House on the Prairie series.  The line was built in 1879-1880 and first operated in the 1880s, and the new railroad line's construction and operation are repeatedly described and featured in the novels.

External links
 Dakota, Minnesota and Eastern Railfan Timetables: Huron Subdivision

Dakota, Minnesota and Eastern Railroad
Chicago and North Western Railway
Rapid City, Pierre and Eastern Railroad
Rail infrastructure in Minnesota
Rail infrastructure in South Dakota
Lotta Stetson Rand (August 26, 1868 – December 3, 1956) was an American social worker and an executive at the American Foundation for the Blind.

Early life and education 
Rand was born in Lynn, Massachusetts, the daughter of John Howard Rand and Julia Dodd Spinney Rand.

Career 
Rand was a social worker in Lynn as a young woman. She became a deputy superintendent with the Massachusetts Commission for the Blind beginning in 1908. She was a delegate to the International Conference for the Blind, held in London in 1914. She  spent three months with the American Red Cross in Halifax in 1918, assigned to report on conditions for blinded victims of the Halifax Explosion. Later in 1918 she went to France to work with the Red Cross in the care of American soldiers blinded in World War I.

In the early 1920s, Rand was executive secretary of the  Harvard Graduate School of Education Course in Education of the Blind, an extension program. In the 1920s and 1930s she was associate director of the American Foundation for the Blind, based in New York. As AFB field representative, she toured in the United States speaking to community groups and raising funds. She often spoke on issues affecting people who became blind in adulthood. 

Rand also made advance arrangements and accompanied some of Helen Keller's speaking engagements in the 1930s. Rand and Keller met as early as 1908, when Keller and Anne Sullivan Macy visited a handicraft shop in Manchester, Massachusetts, run by the Massachusetts Commission for the Blind, Rand was also on hand, as a commission superintendent.

Publications 

 Agencies for the blind in America: Directory of activities for the blind in the United States and Canada (1926, compiled by Rand)

Personal life 
Rand died in 1956, at the age of 88.

References 

1868 births
1956 deaths
People from Lynn, Massachusetts
American social workers
American women in World War I