SIRIS-Lab/unlabelled-sti-corpus huggingface dataset:
Soluble Phosphoinositide Physical Chemistry and the Interpretation of TRPV1 Potentiation

Many cellular signaling processes depend on phosphoinositide lipids (PIPs), so that several techniques to manipulate plasma membrane PIP levels have been developed. Among the most common is the application of “short-chain” PIPs, usually dioctanoyl PIPs, to excised, inside out plasma membrane patches or to whole cells delivered by dialysis through the patch pipette. Our goal has been to better connect studies using these diC8-PIPs to physiological situations.We have applied isothermal titration calorimetry to determine the partition of these lipids into models of both the intracellular and extracellular leaflets of neuronal plasma membranes. To accurately model the measured heats of transfer of the PIPs from solution to the membrane and account for ion binding as the membrane becomes increasingly charged, applied a variable-charge model of the PIP headgroups. We found, not surprisingly, that for equal solution concentrations, the mole fraction of diC8-PI(4)P in the membrane is about twice as high as the mole fraction of diC8-PI(4,5)P2. More intriguing is the implication that very low concentrations give a number of diC8-PI(4,5)P2 are required to activate TRPV1. We will discuss the conditions under which our observations can be extended to physiological PIPs, as well as implications for other ion channel studies applying these short-chain lipids. Our results suggest novel experiments to examine the role of the membrane in regulating membrane protein function.
Better together: Untapped potentials in Central Europe

Borders prevent the optimal exploitation of socio‐economic and environmental resources. A relevant obstacle due to missed integration is associated with legal and administrative barriers, which cause the emergence of untapped potentials. This paper exploits the case study of the Interreg Central Europe programme. Removing borders among countries in the area enhances scale economies due to a large market for inputs and goods, both within regions and across Central European area regions. This paper identifies untapped potentials by looking at the missed regional GDP growth due to the inefficient exploitation of regional growth assets. Results hint at a complex and heterogeneous spatial distribution of untapped potentials, involving several growth factors. Las fronteras impiden la explotación óptima de los recursos socioeconómicos y medioambientales. Un obstáculo relevante debido a la falta de integración se asocia a las barreras legales y administrativas, que provocan la aparición de potenciales no aprovechados. Este artículo aprovecha el estudio de caso del programa Interreg Europa Central. La eliminación de las fronteras entre los países de la zona favorece las economías de escala debido a la existencia de un gran mercado de insumos y bienes, tanto dentro de las regiones como entre las regiones de la zona centroeuropea. El estudio identifica los potenciales no aprovechados mediante el análisis del crecimiento del PIB regional no logrado debido a la explotación ineficiente de los activos de crecimiento regionales. Los resultados apuntan a una distribución espacial compleja y heterogénea de los potenciales no aprovechados, en la que intervienen varios factores de crecimiento. 国境は、社会経済的及び環境的資源の最大限の利用を妨げる。統合の失敗による大きな障害は、法的および行政的な障壁と関連しており、これが「未開発の可能性」の出現を引き起こす。 本稿では、Interreg Central Europeプログラムの事例研究を利用する。地域内の国境を撤廃することは、地域内及び中央ヨーロッパ地域全体の双方において、投入財及び財にとって大きな市場が存在するため、規模の経済を促進する。 本稿では、地域の成長の資源が効率的に活用されないことによって失われた地域のGDP成長を調査することから、未開発の可能性を特定する。結果から、未開発の可能性は、複数の成長要因が関与しており、その空間分布は複雑かつ不均一であることが示唆される。
Maximizing recyclability and reuse of tertiary packaging in production and distribution network

Abstract Tertiary packaging is necessary for transportation in any production and distribution network because of the benefits in enhancing the logistics efficiency. However, in the meantime, it produces a lot of packaging waste every day. In fact, some tertiary packaging after transportation may still be in good condition and can be collected back for reuse. However, this has not been widely studied in the existing literature. The purpose of this research is to fulfill this research gap. Accordingly, the contribution of this paper is to propose a new optimization methodology, named modified Genetic Algorithm with Crossing Date heuristic, to maximize the collection of used tertiary packaging for reuse, meanwhile minimize the total operating cost by taking the advantages of simultaneous optimization of a multi-day planning. From the numerical experiments, it is found that the optimization ability of proposed new optimization methodology outperform the traditional genetic algorithm by a maximum of about 10%. In addition, the total operating cost is found can be reduced by using the proposed multi-day planning approach.
NATO Enlargement: Is Romania Ready to Join the Alliance?

Abstract : Romania's efforts to overcome the legacy of its communist past have been more painful and have proceeded with far less dispatch than in most other East European countries. The autocratic and often cruel brand of socialist ideology practiced by Nicolae Ceausescu left the population physically and mentally malnourished. While Ceausescu and his wife were quickly disposed of on December 25, 1989, following a violent popular upheaval, the residual effects of years of enforced political inactivity and over-centralized planning left the country's social and political elites ill-prepared to manage the new paradigm with which they were faced. As a result, the transition to a true democratic form of government and functioning market economy has proceeded at a halting pace. Through it all, Romanians cast their gaze westward, transfixed on the notion of one day earning the right to be a full-fledged member of NATO.
Re(Defining) cd4+ t cell identities one cell at a time

The immune system consists of a complex continuum of cell types that communicate with each other and non-immune tissues in homeostasis, and during infections, autoimmunity and cancer. Conventional transcriptional and functional profiling enabled by cell surface marker sorting has revealed a great deal about how specific cell types operate en masse, yet important transcriptional heterogeneity that exists within cell populations remains unexplored. High-throughput single cell RNA-seq can overcome this limitation by profiling entire transcriptomes of thousands of individual cells, revealing cell-to-cell variation by decoding patterns within populations masked in bulk transcriptomes. We will exploit this to dissect the mouse CD4+ T cell compartment, a heterogeneous white blood cell population that initiates adaptive immune responses.
In AIM 1, we will chart the dynamics of in vivo CD4+ cell states in mouse before, during and after immune response challenges. By sequencing thousands of single cell transcriptomes, we will map the landscape of CD4+ T cell states in an unbiased, quantitative and comprehensive way. 
In AIM 2, we will predict key transcription factors, cell surface markers, and signalling molecules, including cytokines/chemokines in each cell state through novel computational approaches. Furthermore, our analyses will establish regulatory modules and networks of gene-gene interactions active in immune responses. 
In AIM 3, we will (a) confirm the in vivo impact of new cell states by performing adoptive cell transfer assays; and 
(b) validate our predictions of regulatory molecules and interactions using a massively parallel CRISPR/Cas knockout screen in vitro. 
This powerful integrated approach combines single cell RNA-sequencing, bioinformatics and genetic engineering to dissect CD4+ T cell states, a central compartment of mammalian adaptive immunity, and reveal basic principles of gene regulation.
Nitrogen-Enriched plasma activated water, an unparalleled plant feeding system

Horticultural producers fertilise their crops with Nitrogen (N) to ensure healthy and abundant yields that meet the needs of an increasing population. EU greenhouse farmers spend € 808 M yearly in synthetic N-fertilisers, which are energy-intensive to produce, heavily dependent on fossil fuels and very pollutant. Moreover, the supply chain of synthetic N-fertilisers entails a great amount of GHG emissions, with costs estimated at €14 B/yr. Fertilisation with synthetic fertilisers is unsustainable particularly in EU as the price of N is highly dependent on changes in the price of natural gas. 
VitalFluid brings to market a sustainable and non-polluting alternative to current synthetic N-fertilisers. Our PLAsma Nitrogen FIXation technology provides an on-the-spot production of liquid N-fertiliser that makes the current supply chain of fertilisers redundant. Only with air, water and electricity from renewable sources, our innovative patented plasma activation technology extracts N directly from the air and dissolve it into Plasma Activated Water (PAW) which is nitrate-enriched water ready to be supplied to crops. PLAN2FIX will provide the agriculture sector and society with lower cultivation costs and third parties’ dependency, ensured availability of fertilisers and a drastic reduction of GHG emissions. 
With 500,000 Ha of greenhouses in the world and 210,000 Ha in EU, our short-term target customers are professional greenhouse horticulture growers which require more fertilisers per hectare due to intensive cultivation practice. We aim at entering leading countries in the greenhouse and advanced farming: Netherlands, Germany, Spain, France, Italy, Poland & UK in the first 5 years. We will sell directly in NL while will rely on a distribution network outside NL. From 2022 until 2026, we expect 450 units sold, €44.5 M of cumulated revenue, EBITDA of € 22M and a ROI(5Y) of 5.53. The payback of the investment is less than 2 years and we will create 22 new job positions.
COLOR-MAGNITUDE RELATIONS WITHIN GLOBULAR CLUSTER SYSTEMS OF GIANT ELLIPTICAL GALAXIES: THE EFFECTS OF GLOBULAR CLUSTER MASS LOSS AND THE STELLAR INITIAL MASS FUNCTION

Several recent studies have provided evidence for a `bottom-heavy' stellar initial mass function (IMF) in massive elliptical galaxies. Here we investigate the influence of the IMF shape on the recently discovered color-magnitude relation (CMR) among globular clusters (GCs) in such galaxies. To this end we use calculations of GC mass loss due to stellar and dynamical evolution to evaluate (i) the shapes of stellar mass functions in GCs after 12 Gyr of evolution as a function of current GC mass along with their effects on integrated-light colors and mass-to-light ratios, and (ii) their impact on the effects of GC self-enrichment using the 2009 `reference' model of Bailin & Harris. As to the class of metal-poor GCs, we find the observed shape of the CMR (often referred to as the `blue tilt') to be very well reproduced by Bailin & Harris' reference self-enrichment model once 12 Gyr of GC mass loss is taken into account. The influence of the IMF on this result is found to be insignificant. However, we find that the observed CMR among the class of metal-rich GCs (the `red tilt') can only be adequately reproduced if the IMF was bottom-heavy (e.g., $-3.0 <~ \alpha <~ -2.3$ in $dN/dM \propto M^{\alpha}$), which causes the stellar mass function at subsolar masses to depend relatively strongly on GC mass. This constitutes additional evidence that the metal-rich stellar populations in giant elliptical galaxies were formed with a bottom-heavy IMF.
New reagents for quantifying the routing and kinetics of t-cell activation

The activation of The activation of cytotoxic T-cells (CTLs) marks the key step in the adaptive immune response against cancer and viral infections. Vaccines that activate the CTL-response against tumours are currently hotly pursued, with those targeting tumour-specific mutations now reaching the clinic. However, the capacity of these vaccines to actually activate CTLs is low and rational design improvements are needed to broaden their application. For this, better in vivo knowledge on the processes leading to CTL-activation is needed. 

CTLs are activated by dendritic cells. These cells take up the vaccine, and process it to 8-10-mer peptides for presentation to CTLs on MHC-I complexes. A process called cross-presentation. In this proposal, I aim to quantify key unknowns of this pathway, relating to its kinetics and the subcellular route a vaccine takes to reach the MHC-loading site. I will:  

a)  Quantify the kinetics of peptide-MHC appearance, persistence and disappearance, in vivo using vaccines carrying bioorthogonal protecting/blocking groups that allow me to chemically activate, and stop, the recognition by CTLs in time. 
b) I will also study the sub-cellular route(s) the vaccine antigens take in vivo, by developing a second family deprotection and blocking reagents that are targeted to specific organelles. 

This localised vaccine activation (or de-activation) will allow me to study the different proposed subcellular routes of the antigen in isolation and assess the importance of the many proposed subcellular routes in vivo to overall cross-presentation success. 

The kinetic and routing information obtained from this new set of reagents, will allow me to shed light on some of the key unknowns relating to cross-presentation in vivo, and assess their importance to the success of CTL-activation. By using vaccine-models closely related to existing cancer vaccines, these data will in turn serve to support the rational design of better anti-tumour vaccines.
Concerted action epbd v

Concerted Action EPBD V aim to support the implementation of the Energy Performance in Buildings Directive through exchange of information and experience among Member States and other participating countries (Norway) with regards to the implementation of the specific European Union legislation and policy on the energy performance of buildings, and in particular with regards to the transposition and implementation of the re-cast of the EPBD (DIRECTIVE 2010/31/EU) and the on-going revision of this directive.
  
The specific objectives are to: 
1. Enhance and structure sharing of information and experience from national implementation and promote good practice in activities required of Member States for implementation of the Energy Performance of Buildings Directive (EPBD).  
2. Create favourable conditions for faster convergence of national procedures on EPBD-related matters. 
3. Develop a direct link with the other two buildings-related Concerted Actions established within the IEE programme: the CA-RES, focussing on transposition and implementation of the Renewable Energy Systems Directive (DIRECTIVE 2009/28/EC); and the CA-EED, focusing on transposition and implementation of the Energy Efficiency Directive (DIRECTIVE 2012/27/EU), where National Energy Plans include initiatives towards building energy efficiency. 
4. Supplement the work of the Article 26 Committee and possible ad-hoc groups on CEN (European Committee for Standardization) standards and certification exercises. 
5. Establish a dialogue with the CEN in the implementation of 2nd generation standards to support the implementation of the recast EPBD and its revision. 
6. Support for European Member States and Norway to use National Energy Plans to report progress on the EPBD implementation. 

The CAV_EPBD will strive to result in a more harmonized approach, improved implementation and actual application of the EPBD in all the countries involved, as well as helping to disseminate best practices between the countries.
Correlation of the clinical and physical image quality in chest radiography for average adults with a computed radiography imaging system

The purpose of this study was to examine the correlation between the quality of visually graded patient (clinical) chest images and a quantitative assessment of chest phantom (physical) images acquired with a computed radiography (CR) imaging system.The results of a previously published study, in which four experienced image evaluators graded computer-simulated postero-anterior chest images using a visual grading analysis scoring (VGAS) scheme, were used for the clinical image quality measurement. Contrast-to-noise ratio (CNR) and effective dose efficiency (eDE) were used as physical image quality metrics measured in a uniform chest phantom. Although optimal values of these physical metrics for chest radiography were not derived in this work, their correlation with VGAS in images acquired without an antiscatter grid across the diagnostic range of X-ray tube voltages was determined using Pearson's correlation coefficient.Clinical and physical image quality metrics increased with decreasing tube voltage. Statistically significant correlations between VGAS and CNR (R=0.87, p<0.033) and eDE (R=0.77, p<0.008) were observed.Medical physics experts may use the physical image quality metrics described here in quality assurance programmes and optimisation studies with a degree of confidence that they reflect the clinical image quality in chest CR images acquired without an antiscatter grid.A statistically significant correlation has been found between the clinical and physical image quality in CR chest imaging. The results support the value of using CNR and eDE in the evaluation of quality in clinical thorax radiography.
ANALOG OUTPUT DIAGNOSIS TOOL

A system, method, and an apparatus related to a diagnostic tool to recognize abnormalities in the readings from the transmitter device. The diagnostic tool may diagnose the health of the transmitter device through review of an analog output and comparing it to a digital value. This comparison may be done periodically. The system includes a device for detecting field device operational anomalies of a field device in an industrial process. The system includes a storage medium operable to store program code and a processor operable to read the program code and operate as instructed by the program code. The program code includes acquiring an analog process variable (PV) value from the field device, acquiring a digital process variable (PV) value from the field device, calculating a difference value between the analog PV value and the digital PV value, comparing the difference value to a first threshold alert value, and issuing an alert to a user if the difference value exceeds the first threshold alert value.
MitoLoc: A method for the simultaneous quantification of mitochondrial network morphology and membrane potential in single cells

Mitochondria assemble into flexible networks. Here we present a simple method for the simultaneous quantification of mitochondrial membrane potential and network morphology that is based on computational co-localisation analysis of differentially imported fluorescent marker proteins. Established in, but not restricted to, Saccharomyces cerevisiae, MitoLoc reproducibly measures changes in membrane potential induced by the uncoupling agent CCCP, by oxidative stress, in respiratory deficient cells, and in ∆fzo1, ∆ref2, and ∆dnm1 mutants that possess fission and fusion defects. In combination with super-resolution images, MitoLoc uses 3D reconstruction to calculate six geometrical classifiers which differentiate network morphologies in ∆fzo1, ∆ref2, and ∆dnm1 mutants, under oxidative stress and in cells lacking mtDNA, even when the network is fragmented to a similar extent. We find that mitochondrial fission and a decline in membrane potential do regularly, but not necessarily, co-occur. MitoLoc hence simplifies the measurement of mitochondrial membrane potential in parallel to detect morphological changes in mitochondrial networks. Marker plasmid open-source software as well as the mathematical procedures are made openly available.
GB5 – A Linking Code between MCNP5 and ORIGEN2.1 for Fuel Burnup and Radiotoxicity Analysis – DEN/UFMG Version

GB is a linking code developed at the Departamento de Engenharia Nuclear/UFMG to couple MCNP with the depletion and burnup capability of ORIGEN2.1. In the first version of GB, code described the behavior during the burn up of only 25 isotopes. The amount of isotopes to be considered in the simulation was increased in the GB version used in this paper (named GB5). It was simulated 75 time steps at 800kw of a Heat Pipe Power System model. Results showed that GB5 is able to generate very similar results compared to MCNPX2.6.0. The small difference encountered with the neutron flux parameter between GB5 and MCNPX2.6.0 is explained by the form that recoverable energy per fission is calculated in GB5. Radiotoxicity and radioactivity results are also presented.
The arm-b skin-allergy diagnostics patch is a compact, easy to apply, low-cost allergy testing product.

Allogen Biotech is innovating the ARM-B allergy diagnostics patch technology for use in the healthcare sector and home-test markets. The company’s advanced biosensor concept draws on the simplicity and reliability of traditional skin-prick procedures, yet provides improved clinical outcomes and patient experience. The Allogen innovation is engineered as 50 mm stick-on disc-shaped patch, composed of a microarray device, which identify panels of allergic reactions. Each type of allergic response is interpreted as electrical impedance changes at individual biosensors. The allergy specialist acquires the test results in 5 minutes on a wide panel of common or specific allergens. The sensors register the degree of severity of an allergic reaction and may provide further information on specific testing. The ARM-B system offers unique advantages for the allergy therapist and patients alike. The patch device is non-intrusive – no need to pen-mark or prick large areas of skin: ARM-B microarray extends only 30 mm. The tine-microelectrode structure of the biosensor component ensures ultra-low invasiveness, penetrating only a few hundred microns of epidermis, not millimetre skin-prick depths. Therefore the ARM-B test offers a pain free consultation. The in vivo patch uses only picomole/nL, of a purified allergen microgel, minimising the risk of adverse side effects prevalent with skin-pricks – viz. nausea, raining, malaise. The device offers a large allergy dataset, a consequence of the microarray concept. The sensors are quantitative, so data on degree of allergic response or severity is collated. The solution to skin-oriented allergy diagnostics is applicable to several alternative market segments, not only accredited clinics and general practice with specialist allergy practitioners, but also in the self-test or home-use setting. ARM-B is a high value, low-cost solution for allergy diagnostics.
Subvention du fonds de roulement pour la cosmétologie esthétique de la Madame MSc Agnieszka Dyl-Chmiel

Le projet vise à aider l’entreprise à fournir des liquidités et à soutenir ses activités quotidiennes en raison de difficultés financières survenues dans l’entreprise à la suite de la pandémie de COVID-19. Assistance financière fournie sur la base du régime SA.57015.