SIRIS-Lab/unlabelled-sti-corpus huggingface dataset:
Mechanistic analysis of dna damage signaling and bypass upon replication of damaged dna template in human cells.

The genetic information encoded by DNA is under constant attack from both endogenous and exogenous sources of damage. To ensure genome stability and prevent disease, cells use global signaling networks to sense and repair DNA damage. One particularly serious problem is when the replication machinery encounters lesions remaining in the template DNA. In this scenario, cells employ damage bypass mechanisms to complete genome replication and prevent fork breakage. Importantly, these pathways are not restricted to the site of stalling but can also function behind the fork at single-stranded DNA (ssDNA) gaps originated by the re-priming of DNA synthesis downstream of lesions. While it is very well known that ssDNA is the molecular signal that triggers the checkpoint response, it is less clear how and where ssDNA actually arises. Generally, it is assumed to accumulate at stalled replication forks, either by an uncoupling between replicative helicase and polymerase movement or between leading and lagging strand synthesis. However, in a recent study in budding yeast, I found that ssDNA gaps left behind replication forks, and extended by processing factors such as the exonuclease EXO1, constitute the predominant signal that leads to checkpoint activation in response to damaged DNA templates during S phase. Whether this mechanism of checkpoint activation is conserved from yeast to humans remains unexplored. Hence, using a unique set of multidisciplinary approaches, this project aims to address the fundamental question of how DNA damage is sensed during replication in human cells. Interestingly, not only ssDNA gap processing seems important for checkpoint signaling but also for the template switching mechanism of damage bypass. Therefore, this project will also study the function of EXO1 and its association with PCNA at postreplicative ssDNA gaps in order to shed light on the poorly understood mechanism of template switching.
Microbiota-Gut-Brain engineered platform to evaluate intestinal microflora impact on brain functionality

What is the relation between the intestinal microflora and the brain in the development of neurodegenerative disorders like Alzheimer's and Parkinson's disease? 
A functional relation between the intestinal microflora (microbiota) and the brain, referred to as the “microbiota-gut-brain axis”, was hypothesized more than 100 years ago but only recently has been re-evaluated becoming a new, exciting hypothesis in neuroscience. We have the proof that aging, bad alimentary habits, poor food quality and stress can affect our intestinal microbiota: the impact of this modification on brain functionality has been observed but the absence of suitable research tools does not allow verifying this research hypothesis. My aim is to overcome this limitation through a bioengineering approach: I will develop the first microbiota-gut-brain platform relying on three miniaturized microfluidic compartments, representing in vitro the most important features of microbiota-brain interaction. Once validated, my platform will be challenged in healthy and neurodegenerative scenarios by using human complete microbiota. MINERVA has a ground breaking potential: the proof of a causal link between intestinal microbiota and brain functionality would completely change the actual scenario, shifting the investigation of neurodegenerative disorders causes from the brain to the body periphery. MINERVA is a high gain project: if its vision succeeds, neurodegenerative disorders will benefit from new, cost effective, low invasive preventative and therapeutic strategies based on microbiota management by food ingredient and probiotics with an enormous beneficial impact worldwide. MINERVA will provide a versatile platform that will overcome the strong limitations arising from the current in vivo models and standard in vitro tools: by changing cell type or culturing conditions, it could address microbiota impact not only on neurodegenerative disorders, but also on other severe other-than-nervous pathologies.
Gold(I)-Catalyzed polymerization of acetylene gas

GASGOLD aims for the development of efficient Au(I)-catalysed methodologies for the challenging intermolecular synthesis of important monomers, oligomers and polymers of acetylene gas. Au(I)-catalysts allow for the formation of cyclopropyl Au(I)-carbenes after reaction with an alkyne and alkene. The carbenes are reactive towards rearrangement to form 1,3-dienes, which are important building blocks for a range of natural and other important products, such as synthetic rubbers. Preliminary results show that further reaction of certain 1,3-dienes with acetylene can occur, forming oligomeric derivatives and this reactivity will be harnessed and further exploited in this project. Initially, the development of an effective protocol for the synthesis of a wider range and more challenging substituted 1,3-dienes from acetylene gas, both in monomeric and oligomeric forms, will be established. The reaction mechanism determining the configuration of the alkenes will be studied, for example by kinetic monitoring and isolation of reaction intermediates, to explain the observed selectivity, potentially synthesise other isomers and improve on the reaction conditions and catalyst design. The initial results will inspire the challenging selective expansion of 1,3-dienes with acetylene to form substituted 1,3,5-hexatrienes. Additionally, the unprecedented use of both acetylene and ethylene gas in Au(I)-catalysis will be explored for the synthesis of polyacetylene derivatives, which are highly important materials with electrical conductivity, as well as in the purification of ethylene gas. The development of this project offers an innovative solution for the long-standing problem of utilising acetylene gas as a simple building block in Au(I)-catalysis, offering a clear advantage over the current state-of-the-art of the field. Furthermore, the expected synergistic performance between the experienced researcher and the host group, will maximise the potential for success of the project.
Hypoxia triggers short term potentiation of phrenic motoneuron discharge after chronic cervical spinal cord injury

Abstract Repeated exposure to hypoxia can induce spinal neuroplasticity as well as respiratory and somatic motor recovery after spinal cord injury (SCI). The purpose of the present study was twofold: to define the capacity for a single bout of hypoxia to trigger short-term plasticity in phrenic output after cervical SCI and to determine the phrenic motoneuron (PhrMN) bursting and recruitment patterns underlying the response. Hypoxia-induced short term potentiation (STP) of phrenic motor output was quantified in anesthetized rats 11 weeks following lateral spinal cord hemisection at C2 (C2Hx). A 3-min hypoxic episode (12–14% O2) always triggered STP of inspiratory burst amplitude, the magnitude of which was greater in phrenic bursting ipsilateral vs. contralateral to C2Hx. We next determined if STP could be evoked in recruited (silent) PhrMNs ipsilateral to C2Hx. Individual PhrMN action potentials were recorded during and following hypoxia using a “single fiber” approach. STP of bursting activity did not occur in cells initiating bursting at inspiratory onset, but was robust in recruited PhrMNs as well as previously active cells initiating bursting later in the inspiratory effort. We conclude that following chronic C2Hx, a single bout of hypoxia triggers recruitment of PhrMNs in the ipsilateral spinal cord with bursting that persists beyond the hypoxic exposure. The results provide further support for the use of short bouts of hypoxia as a neurorehabilitative training modality following SCI.
Engage society for risk awareness and resilience

Natural and man-made disasters remind us how the ability of societies to adapt and prosper depends on the collective action of the whole society. But the significant role citizens and communities can play at the grassroots level has been overlooked in research. ENGAGE will turn this around, showing how individuals and local practices can interrelate effectively with planned preparedness and response, practitioners and technology. To achieve this bold goal ENGAGE will start with the knowledge, strategies, methods, tools and practices used by real world practitioners and citizens, and mature results from earlier projects. It will combine and extend these to create innovative solutions to disaster management and new ways of fostering trans-disciplinary collaboration and learning across disciplines. A model for assessing and methods for improving societal resilience will be complemented by an evolving knowledge platform providing actionable solutions meeting the diverse needs of authorities, first responders and citizens. ENGAGE will use empirical data on individual and collective contributions to societal resilience and take into account contextual aspects such as socio-economic conditions, digital literacy, culture, gender, social capital, trust and diversity. It will focus on aspects that can be directly enhanced such as risk awareness, communication, social media, citizens’ as well as authorities’ and first responders’ involvement. Real world field validations will be used to demonstrate and validate ENGAGE solutions and their transferability to diverse contexts across Europe. The consortium is complemented by a Knowledge and Innovation Community of Practice that already has 37 members and will grow during the project. This team includes representatives from authorities, first responders, citizens associations, NGO´s, SMEs, industries, schools and academia. Together, they will propose validated solutions contributing to specific SENDAI actions.
Linking atomic-scale properties of 2d correlated materials with their mesoscopic transport and mechanical response

Fundamental material properties become highly susceptible to external perturbations in low dimensions. This presents tremendous new opportunities for manipulating the behavior of novel 2D layered materials and ultimately achieving unprecedented control over their performance when integrated into highly specific functional devices. However, strategies that enable such control are sorely lacking to date and remain an outstanding challenge for the materials science community. Progress here requires of a comprehensive microscopic picture of the fundamental properties of 2D materials in clear connection to their macroscopic behavior, a knowledge that is still missing due to the lack of experimental techniques that simultaneously probe multiple length regimes. 

The main objective of the proposed research is to demonstrate control over the electronic ground states of 2D materials via external strain and electromagnetic fields to build links of applicability for signal processing in electromechanical nanodevices. We will focus on 2D correlated materials exhibiting collective electronic phases such as superconductivity, which respond dramatically to external perturbations. The project aims to understand the interplay between these external stimuli and microscopic electronic phases, and to unambiguously correlate them with mesoscopic electrical transport and mechanical response. This project comprises three research thrusts: (i) Development of new instrumentation that provides a direct way to correlate atomic-scale and mesoscopic properties of materials, and to establish links between (ii) the electrical conductivity and (iii) the mechanical response of 2D correlated materials with their atomic-scale structure and stimulus-dependent electronic phase diagram. This project has the potential to transform this field by providing new pathways to control the behavior of layered nanostructures.
Anglo-German Anthropology in the Malay Archipelago, 1869–1910:Adolf Bernhard Meyer, Alfred Russel Wallace and A.C. Haddon

The indigenous inhabitants of New Guinea and surrounding islands, along with so-called 'Negrito' groups in mainland Southeast Asia and the Malay Archipelago, stood at the forefront of European anthropological and ethnological interest during the second half of the nineteenth century. German researchers interested in these groups frequently engaged in transnational dialogue and debate: they read, reviewed and translated one another's work, corresponded on matters of interest, and corroborated or contradicted one another's conclusions. This chapter illuminates these scholarly connections by focusing on the German traveller-naturalist and museum director Adolf Bernhard Meyer. His connections with English-speaking colleagues, particularly the naturalist Alfred Russel Wallace and the anthropologist Alfred Cort Haddon, shed light on the mutually constitutive nature of metropolitan knowledge and field experience, the role of translation in Anglo-German scholarly dialogue, and the variable interpretation of anthropological data within different national cultures of scientific knowledge. Keywords: Adolf Bernhard Meyer; Alfred Cort Haddon; Alfred Russel Wallace; Anglo-German scholarly dialogue; European anthropological interest; European ethnological interest; Malay Archipelago; Negrito groups; New Guinea; Southeast Asia
AN ANTENNA HAVING AN ANTENNA TO RADOME RELATION WHICH MINIMIZES USER LOADING EFFECT

An antenna is provided, which is located within an enclosure. The antenna includes one or more arms, where each arm has an electrical length corresponding to an intended frequency band of transmission, and along said length of the arm a source of external loading will have a variable effect. The enclosure includes one or more anticipated points of contact, where a source of external loading will be brought into proximity with said enclosure, and where the one or more arms are constructed and arranged to locate the relatively high impedance areas of the antenna at least a predetermined distance from the one or more anticipated points of interest, and the relatively low impedance areas of the antenna are located more proximate the anticipated points of interest.
Molecular motors, powering dynamic functional molecular systems

In this proposal the unique properties of unidirectional light driven molecular rotary motors will be built upon to achieve dynamic control of function and develop responsive systems with a particular focus on systems in water. Light-driven molecular rotary motors are distinct from the majority of molecular switches, as they allow sequential access to multiple functional states in a responsive system through non-invasive stimulation. Importantly, continuous irradiation induces continuous rotary motion which provides a unique opportunity to design dynamic systems and responsive materials that can be driven out-of-equilibrium. The research program is divided in four work-packages: a) chemical and redox driven unidirectional motors; here we will develop processive unidirectional motors that can use (electro)chemical energy in a continuous manner, b) amplification of motion; here rotary motors operate in assemblies to amplify mechanical function over a wide range of length scales. Specifically we will use liquid crystal-water interfaces as a unique platform to control motion and organization. c) dissipative self-assembly: molecular motors offer fantastic opportunities to control self-assembly and drive such systems out-of-equilibrium. We aim at metastable aggregate formation (hydrogels) and the design of amphiphilic motors for responsive self-assembled nanostructures; d)triggering biomolecular function; the goal is to use rotary motors to regulate DNA transcription and ultimately as genuine powering device to control cardiac cell function. In the emerging field of photopharmacology, we take advantage of non-invasive high spatio-temporal control that switching with light provides.  The proposed research program is highly challenging but provides the comprehensive effort required to achieve control of complex nanomechanical systems and will opening a bright future for applications ranging from stimuli responsive materials to spatio-temporal control of biomolecular systems
HCV Genotyping from NGS Short Reads and Its Application in Genotype Detection from HCV Mixed Infected Plasma

Genotyping of hepatitis C virus (HCV) plays an important role in the treatment of HCV. As new genotype-specific treatment options become available, it has become increasingly important to have accurate HCV genotype and subtype information to ensure that the most appropriate treatment regimen is selected. Most current genotyping methods are unable to detect mixed genotypes from two or more HCV infections. Next generation sequencing (NGS) allows for rapid and low cost mass sequencing of viral genomes and provides an opportunity to probe the viral population from a single host. In this paper, the possibility of using short NGS reads for direct HCV genotyping without genome assembly was evaluated. We surveyed the publicly-available genetic content of three HCV drug target regions (NS3, NS5A, NS5B) in terms of whether these genes contained genotype-specific regions that could predict genotype. Six genotypes and 38 subtypes were included in this study. An automated phylogenetic analysis based HCV genotyping method was implemented and used to assess different HCV target gene regions. Candidate regions of 250-bp each were found for all three genes that have enough genetic information to predict HCV genotypes/subtypes. Validation using public datasets shows 100% genotyping accuracy. To test whether these 250-bp regions were sufficient to identify mixed genotypes, we developed a random primer-based method to sequence HCV plasma samples containing mixtures of two HCV genotypes in different ratios. We were able to determine the genotypes without ambiguity and to quantify the ratio of the abundances of the mixed genotypes in the samples. These data provide a proof-of-concept that this random primed, NGS-based short-read genotyping approach does not need prior information about the viral population and is capable of detecting mixed viral infection.
AUTOMATED PROCESSING OF BULK CONTAINERS

A system and method is used to automatically process the contents of a containers (212). The contents may be bulk materials used by a business or organization. The container (212) enters the system via a tow assembly mechanism (210), a grasping mechanism (222)clasps the container, a lid (220) secures the contents, the container (212) is lifted and rotated to allow the contents of the container (212) to exit the container(212).
TEMPERATURE SENSITIVE POLYMERS AND WATER-DISPERSIBLE PRODUCTS CONTAINING THE POLYMERS

The present invention is directed to a novel process of making N-isopropyl acrylamide (NiPAm) polymers based upon the reaction of poly(acrylic acid) and N-isopropylamine. The disclosed method of the present invention uses polyacrylic acid as a starting material to synthesize NiPAm polymers. In one embodiment, the present invention discloses a condensation reaction of an intermediate salt to form homopolymers, copolymers and terpolymers of Nisopropyl acrylamide (NiPAm) with acrylic acid and/or alkyl acrylates in a molten state, which is adaptable to a continuous reactive extrusion process. Binder compositions, water-dispersible products and thermoformable articles containing the NiPAm polymers are also disclosed.
Factors affecting parental supply of alcohol to underage adolescents

The aim of this study was to determine the extent to which parent and adolescent characteristics predict parental supply of full serves of alcohol (i.e. not simply sips) to their adolescent aged 14-16 years.In 2009, a cross-sectional sample of 388 parents from Victoria, Australia was surveyed.Of the 70% of parents who believed that their adolescent currently drinks, 37% reported supplying their underage adolescent with more than a sip of alcohol in the last 3 months. Alcohol supply was significantly associated with parents' perceptions that their adolescent drinks, odds ratio 1.87 (95% confidence interval 1.38-2.53) and higher levels of parental monitoring, odds ratio 1.44 (95% confidence interval 1.10-1.94) but not significantly associated with parent/adolescent sociodemographic characteristics or parents' drinking patterns.Consistent with reports from Australian students, parents are a major source of supply of alcohol to underage adolescents. While there are legislative and policy guidelines regarding the use of alcohol by underage adolescents, parents need support to implement and reinforce alcohol-specific rules for their children.
Nuevas tecnologías para motores eléctricos de bobinado en Elvin PRODEJ s.r.o.

El objeto del proyecto es la adquisición de tecnología de última generación para la reparación y mantenimiento de equipos eléctricos — motores eléctricos industriales, transformadores e imanes de carga. Gracias a la nueva tecnología, la empresa será capaz de realizar pedidos aún más exigentes de dimensiones más grandes (incluyendo motores eléctricos de más de 1 tonelada), podrá obtener salidas específicas durante el diagnóstico de la máquina para optimizar el trabajo de servicio. El beneficio básico del proyecto es aumentar la flexibilidad de la empresa y reducir al mínimo el tiempo de servicio.
CONTINUOUS ON-BOTTOM DIRECTIONAL DRILLING METHOD AND SYSTEM

A method of and system for directional drilling alternate between rotary drilling and sliding with the bit remaining in continuous contact with the bottom of the bore hole.
The question about questions: is DC a good choice to address the challenges of representation of clinical research questions and value sets?

Question and answer sets are the core of clinical research. The [RD] PRISM (Patient Registry Item Specifications and Metadata for Rare Disease) project will provide a library of standardized questions across a broad spectrum of rare diseases that can be used for a variety of clinical information and data collection purposes, such as registries. Questions will be encoded using well-established clinical terminologies to enable cross-indication and cross-disease analyses, facilitate collaboration, and generate meaningful results for rare disease patients, physicians, and researchers. Encoded question and answer sets will also be indexed to facilitate information retrieval by subject matter, data type, and time interval. This project will outline issues and challenges related to indexing questions for future use and for data sharing; to explore possible metadata and terminological standards for indexing them; and, determine if Dublin Core (DC) is a viable alternative to other schemes for a library of standardized rare disease research questions.
Reduced Dynamic Orbit Determination Using Differenced Phase in Adjacent Epochs for Spaceborne Dual-frequency GPS

Abstract We study on reduced dynamic orbit determination using differenced phase in adjacent epochs for spaceborne dual-frequency GPS. This method not only overcomes the shortcomings that the epoch-difference kinematic method cannot be used when observation geometry is poor or observations are insufficient, but also avoids solving the ambiguity in the zero-difference reduced dynamic method. As the epoch-difference method is not sensitive to the impact of phase cycle slips, it can lower the difficulty of slip detection in phase observation preprocessing. In the solution strategies, we solve the high-dimensional matrix computation problems by decomposing the long observation arc into a number of short arcs. By gravity recovery and climate experiment (GRACE) satellite orbit determination and compared with GeoForschungsZentrum (GFZ) post science orbit, for epoch-difference reduced dynamic method, the root mean squares (RMSs) of radial, transverse and normal components are 1.92 cm, 3.83 cm and 3.80 cm, and the RMS in three dimensions is 5.76 cm. The solution's accuracy is comparable to the zero-difference reduced dynamic method.