SIRIS-Lab/unlabelled-sti-corpus huggingface dataset:
Dna-Origami frame platform for co-evolution ligand selection

Affinity reagents such as antibodies and aptamers are of paramount importance as tools in biotechnology and the treatment of a wide range of diseases. However, despite their scientific and economAffinity reagents such as antibodies and aptamers are of paramount importance as tools in biotechnology and the treatment of a wide range of diseases. However, despite their scientific and economic impact it remains challenging to systematically generate high-affinity ligands to cover all epitopes of a given target. Here I propose a strategy to provide spatial control over ligand discovery process leveraging the power of in vitro evolution in conjunction with the spatial addressability of DNA nanotechnology methods. Together they form a new discovery platform for the systematic and parallel generation of ligands, specifically aptamers and single-chain Fv antibody fragments (scFv), to provide coverage of epitopes at predefined targets. The proposed strategy exploits cooperative binding (avidity), by co-evolution of the affinity reagents, in the context of a defined molecular three-dimensional framework provided by DNA origami structures. This further expedites structure determination of ligand-target complexes by cryo-electron microscopy (cryo-EM). The combination of rational framework design, in vitro evolution and structural feedback provided by cryo-EM reconstructions will provide a toolbox for the systematic generation of ligands to all accessible epitopes. Our approach also provides a tool for electron microscopy reconstruction of smaller (< 100kDa) biomolecules and their molecular interactions by enhancing contrast and providing context. Once established, this approach will provide a transformational technology platform that enables the parallel interrogation of multidimensional, spatially resolved libraries, yielding cooperative ligands for highly specific target recognition, with direct applications in biosensor development, proteome analysis, diagnostics and therapy.
An open-source framework for formal specification and simulation of electrical energy systems

Electrical energy systems (EESs) are systems which consume, generate, distribute and store energy at various scales. This paper presents a modeling and simulation framework that uses principles borrowed from the system-level simulation of digital systems and extends them to the case of EESs. The framework relies on open-source standards such as SystemC (and its Analog and Mixed-Signal extensions) for simulation, and IP-XACT for interface definition. The paper extends the IP-XACT standard with the formal definition of the interfaces of the typical components of an EESs. Then, it proposes a methodology to seamlessly plug such components into the simulation framework using the power configuration information contained in the IP-XACT descriptions. Simulations show that the proposed approach provides accuracy comparable to Matlab/Simulink results, with higher modularity and faster simulation times.
Lo strano caso delle streghe di Sicilia: donne de fora, streghe, fate e guaritrici (XVI‑XIX sec.)

donne de fora, sorcières, fées et guérisseuses (XVI e -XIX e siècle)
Light induced spatially exact and genetically encoded labeling of immune cells for monitoring of long distance and ultra-compartment shuttling during autoimmunity and chronic inflammation

The incidence of autoimmune diseases including multiple sclerosis is dramatically increasing. While there is a genetically defined “bedrock” susceptibility to develop T cell mediated autoimmunity, environmental cues likely determine the threshold for disease development. Yet, little is known on how environmental cues sensed at body/environment interfaces are translated into immunopathology in distant organs like the central nervous system (CNS).
Here, we raise the hypothesis that immune cells must be activated at epithelial surfaces and then physically migrate to distant organs in order to induce autoimmunity. Furthermore, we propose that the “state of activation” of (either lymphoid or myeloid) immune cells can be interrogated by IL-6 production since IL-6 deficiency confers resistance to virtually any organ specific autoimmune disease and we have contributed fundamentally in defining the role of IL-6 for the generation of Th17 cells that are highly associated with autoimmune tissue inflammation.
In EXODUS, we will develop ground-breaking next generation reporter tools in order to test these hypotheses. A split Cre recombinase protein, which dimerizes and is activated by blue light, will be used to genetically label cells (and their progeny) in a topologically defined manner (“compartment reporter”). Furthermore, we have developed a novel type of Cre-inducible in vivo IL-6 reporter (“activation reporter”). The combination of these tools will enable us to trace the anatomical compartment of activation of immune cells without limitations in lag time.
Thus, site specific photogenetic co-induction of a fluorescence and IL-6 reporter will be used to probe peripheral sites for their potency to licence immune cells to travel to the CNS (Forward). Vice versa, labeling of cells in the CNS (through a thinned skull window) will allow for studying immune cell exodus from the CNS in homeostasis and during inflammation (Reverse).
Crosslinked chitosan nanofiber mats fabricated by one-step electrospinning and ion-imprinting methods for metal ions adsorption

The Pb(II) ion-imprinting electrospun crosslinked chitosan nanofiber mats were fabricated by one-step electrospinning and ion-imprinting methods and their application as adsorbents for metal ions was also investigated. The resulting chitosan nanofiber mats were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and thermal gravimetric analysis (TGA). The Pb(II) ion-imprinting electrospun crosslinked chitosan nanofiber mats were used as adsorbents for the removal of Pb(II) ions from aqueous or acid solutions. The effects of pH values, contact time, content of crosslinker (glutaraldehyde) on Pb(II) ions adsorption were studied. The results indicated that the Pb(II) ion-imprinting electrospun crosslinked chitosan nanofiber mats had the highest adsorption capacity of 110.0 mg/g at pH 7. The kinetic study demonstrated that the adsorption of Pb(II) ions followed the pseudo-second-order model. The equilibrium isotherm data showed that the Langmuir model was the most suitable for predicting the adsorption isotherm of the studied system. The Pb(II) ion-imprinting electrospun crosslinked chitosan nanofiber mats had good adsorption selectivity, which illustrates the equilibrium adsorption capacity in the order of Pb(II)>Cu(II)>Zn(II)>Cd(II)>Ni(II). The Pb(II) ionimprinting electrospun crosslinked chitosan nanofiber mats were stable and had good reuse ability.
A Conceptual Model for Developing Mindsets for Strategic Insight Under Conditions of Complexity and High Uncertainty

This article addresses an important, yet often underattended to, aspect of the strategy development process: fostering the use of strategic learning practices in the simultaneous practice of developing strategy and cultivating strategic mindset awareness. The need for addressing this aspect of the strategy development process is increasingly important because of the intensifying complexity of the task environment. Following a framing of this context in terms of a complex adaptive systems perspective, a conceptual model for addressing this strategic HRD challenge is presented. The model is based on two distinct, yet in terms of potential implications for HRD practice, interconnected streams of literature: strategic learning practices, and adult development theory (specifically, constructive developmental theory and developmental action inquiry). The implications for HRD practices seeking to address this need, along with implications for future research, are also discussed.
Microcrystallite dimension and total active surface area of carbon electrode from mixtures of Pre-Carbonized oil palm empty fruit bunches and green petroleum cokes

Carbon pellets (CP) were prepared from the green pellets (GP) containing mixtures of pre-carbonized oil palm empty fruit bunches (SACG) and Green Petroleum Cokes (GPC), with the weight percentages (x) of SACG in the samples at 10%, 30%, 50%, 70% and 90%. Carbonization process to produce the CP was conducted up to 900oC using a multi steps heating profile. The interlayer spacing (d200 and d100), stack height (Lc), stack width (La,) and effective dimension L of the turbostratic crystallites (microsrystallite) in the CPs were estimated from X-ray diffraction data; d200, d100, La, L increased and Lc decreased with increasing weight percentage of SACG. The total surface area of active material of the CP (Atot) with thickness, t, estimated from Lc was found to follow the equation, Atot = [4.8086 – 0.0083x]1010 t, indicating a significant influence of the SACG content in the mixture of the green body.
Combining hyperspectral luminescence imaging and mineralogical identification to date mixed-component samples from volcanic eruptions

Volcanic hazards directly affect more than 10% of the world’s population, and have caused globally significant economic losses as recently as 2010. Accurate eruption frequencies are fundamental to risk management, and well-dated volcanic events can temporally link archaeological sites and palaeoclimatic records across hundreds of kilometres. Yet there are significant, known gaps in records of prehistoric volcanic cycles, and accurate luminescence ages for volcanic features would be of great value in future studies. However, volcanic products are mineralogically complex with some crystals producing inaccurate ages. We propose exploring and developing an innovative luminescence technique (‘spatially resolved luminescence’, ‘SRL’) to date volcanic events. SRL uses an ultra-sensitive camera to image luminescence, so that signals can be easily compared with accompanying experimental data (e.g. mineral type or signal stability measurements). This project utilizes luminescence imaging at multiple wavelengths (‘hyperspectral’) and mineralogical analyses to accurately date volcanic ejecta with mixed mineralogy. It builds on the expertise of the supervisor and experienced researcher (‘ER’) in luminescence dating and imaging as well as statistical data analysis, and enhances the ER’s skill set via training in volcanology, the development of analytical software, and the German language and culture. The ER will draw together an international network of leading scientists in industry (Freiberg Instruments) and academia (Universities of Freiburg, Bern, Oxford), allowing her to substantially enhance her leadership skills. As a subsidiary outcome, this project will also increase the European capacity in multiple connected fields, including composite strain analysis (aerospace) and detection of irradiated foods (food standards authorities).
Populist rebellion against modernity in 21st-century eastern europe: neo-traditionalism and neo-feudalism

POPREBEL is an inter-disciplinary consortium of researchers focused on explaining and contextualising the recent rise of populism in Central and Eastern Europe (CEE). While populism is a phenomenon that has by now emerged in almost every democracy, we believe that regional and cultural-historical dimensions need to be considered in order to improve not only scholarly knowledge, but also policy recommendations. It is urgent for Western Europeans to look into the CEE mirror, just as it is urgent for the CEE region to understand itself. To this end, we will create a typology of populism’s various manifestations, reconstruct trajectories of its growth and decline, investigate its causes, interpret its meanings, diagnose its consequences, and propose policy solutions.

Our focus is on CEE, but we engage in comparisons with populisms elsewhere, particularly Western Europe. While scholars from different disciplines have looked at populism, we argue that the full potential of interdisciplinary research has not been achieved yet. We draw on data and models from various disciplines and combine them into a rich study of forces at play. In addition to established methods of economics, sociology, and cultural studies, at the heart of our approach is a novel methodology whose essence is a large-scale, Europe-wide, multi-lingual online conversation. This is a listening exercise; its aim is a deep understanding of everyday life in Europe challenged by the rise of populism. The method to process this unique ethnographic material is semantic social network analysis.

POPREBEL relies on foresight/future studies, deep involvement with activists, policy makers, and civil society actors to boost the immune system of European democracy. We develop scenarios and share them with all interested parties to reflect, in public debate, on how well they fit both in the CEE region and the rest of Europe. Our focus is not just scholarly; the project serves as a platform for mutual learning.
TERAPIA EPIGENETICA PER IL TRATTAMENTO DELLE INFEZIONI DA CORONAVIRUS

BACKGROUND. IL VIRUS SARS-COV-2 POSSIEDE UN GENOMA A RNA A SINGOLO FILAMENTO. DOPO LÂ INGRESSO NELLA CELLULA BERSAGLIO, IL VIRUS INIZIA LE FASI DI REPLICAZIONE DEL SUO GENOMA E DI SINTESI DELLE PROTEINE NECESSARIE. LE CELLULE EPITELIALI DELLE VIE AEREE E LE CELLULE DELLA IMMUNITÃ  INNATA (MACROFAGI) SONO I PRINCIPALI BERSAGLI DEL VIRUS. DURANTE LA REPLICAZIONE DEL VIRUS SARS-COV-2, VIENE GENERATO RNA A DOPPIA ELICA (DOUBLE-STRANDED RNA, DSRNA) CHE PORTA ALLÂ ATTIVAZIONE DI VARI MECCANISMI DI DSRNA-SENSING. LA RISPOSTA GENERATA INDUCE LA SINTESI DI INTERFERONI, CHEMOCHINE, CITOCHINE E LA MIGRAZIONE IN LOCO DI CELLULE DEL SISTEMA IMMUNITARIO CHE AMPLIFICANO E PERFEZIONANO LA RISPOSTA INIZIALE. I CORONAVIRUS ESPRIMONO PROTEINE CHE SONO IN GRADO DI INATTIVARE I MECCANISMI DI DSRNA-SENSING, INNESCANDO LA DEREGOLAZIONE COMPLESSIVA DELLA RISPOSTA ALLÂ INFEZIONE VIRALE CHE PORTA AD UN ULTERIORE DANNO CHE DERIVA DALLÂ ECCESSIVA INFIAMMAZIONE DEI TESSUTI E DALLA INEFFICACE RISPOSTA DI ALCUNE POP
Émile Durkheim (1858–1917)

In Goffmans Soziologie ist das Vermächtnis Émile Durkheims grundlegend. Er entdeckte das Werk des französischen Soziologen schon in seinen ersten Jahren an der Universität Toronto, als er an einem Lektüreseminar über Le Suicide (1897) teilnahm. Er vertiefte seine Kenntnisse in Chicago dann unter der Leitung von W. Lloyd Warner, seinem späteren Doktorvater. Durkheims Spätwerk Les Formes Élémentaires de la Vie Religieuse (1912) hat besonders stark Goffmans Aufmerksamkeit gebunden. Auch wenn Goffman sich auf Durkheims Analysen stützt, ist seine interaktionistische Interpretation eigenständig. Er verwendet diese für die Analyse der Mikroprozesse im Alltagsleben. In diesem Beitrag werden die zahlreichen Anregungen auf Goffman aufgezeigt und nachgezeichnet, wie er diese in seinen Arbeiten weiterentwickelt hat.
Anion-Binding Properties of a Cyclic Pseudohexapeptide Containing 1,5-Disubstituted 1,2,3-Triazole Subunits

A C(3) symmetric cyclic pseudohexapeptide containing 2-aminopicoline-derived subunits and 1,5-disubstituted 1,2,3-triazole rings is introduced as a potent anion receptor. This macrocycle was designed to mimic both the conformation and the receptor properties of a previously described cyclic hexapeptide containing alternating L-proline and 6-aminopicolinic acid subunits. Conformational analyses demonstrate that the cyclic peptide and the cyclic pseudopeptide are structurally closely related. Most importantly, both exhibit a converging arrangement of the NH groups, hence a good preorganization for anion binding. As a consequence, the pseudopeptide also very efficiently interacts with halide and sulfate ions, and this is the case even in competitive aqueous solvent mixtures. However, there are clear differences in the structures of both compounds, which translate into characteristic differences in receptor properties. Specifically, (i) the pseudopeptide possesses an anion affinity intrinsically higher than that of the cyclopeptide, (ii) the pseudopeptide is well preorganized for anion binding in a wider range of solvents from aprotic to protic, (iii) anion affinity in aprotic solvents is very high and associated with complexation equilibria that are slow on the NMR time-scale, (iv) the propensity of the pseudopeptide to form sandwich-type 2:1 complexes with two receptor molecules surrounding one anion is significantly lower than that of the cyclopeptide. A solvent-dependent calorimetric characterization of the binding equilibria of both compounds provided clear evidence for the stabilizing effect of hydrophobic interactions between the receptor subunits in such 2:1 complexes. The pseudopeptide thus represents the first member of a new family of anion receptors whose properties may be fine-tuned by varying the side chains in the periphery of the cavity.
Conodontos del frasniese superior y frameniense inferior de la formación cardaño (unidad del cildar-montó, dominio palentino, zona cantábrica)

Se describe la sucesión de conodontos del Devónico Superior en torno al límite Franiense-Fameniense en dos secciones del Dominio Palentino (Unidad del Gildar-Montó). Se discuten sus implicaciones estratigráficas con respecto a las muestras aisladas del Frasniense superior referidas en la bibliografía sobre la Formación Cardaño en diferentes localidades de dicho dominio. Los comodontos del Fameniense tienen un gran interés por ser los primeros atributos a la Zona de Palmatolepis triangularis en la zona Cantábrica. Además, estos conodontos se encuentran en un horizonte con el primer registro de ammoideos del genero Falcitornoceras. Se discuten las diferencias entre las biofacies de conodontos obtenidas para el Frasniense y el Fameniense
Strengthening innovation and competitiveness of dairy enterprises through Mobile Laboratories of quality control

The project aims to work with the entire dairy chain from the producers to the consumers, by introducing innovation technology in farming and cheese processing in order to standardize the Ionian and Apulian dairy products with the check of milk and cheese quality. In particular, the project will provide technical assistance to farmers by developing two mobile laboratories in Puglia and Ionian Islands that will enhance the cheese safety and quality level. The projects will involve local producers raisers and businessmen to facilitate commercial exchanges, especially women & young people to create conditions for new qualified jobs as opportunities.
Positional Assembly of Enzymes on Bacterial Outer Membrane Vesicles for Cascade Reactions

The systematic organization of enzymes is a key feature for the efficient operation of cascade reactions in nature. Here, we demonstrate a facile method to create nanoscale enzyme cascades by using engineered bacterial outer membrane vesicles (OMVs) that are spheroid nanoparticles (roughly 50 nm in diameter) produced by Gram-negative bacteria during all phases of growth. By taking advantage of the fact that OMVs naturally contain proteins found in the outer cell membrane, we displayed a trivalent protein scaffold containing three divergent cohesin domains for the position-specific presentation of a three-enzyme cascade on OMVs through a truncated ice nucleation protein anchoring motif (INP). The positional assembly of three enzymes for cellulose hydrolysis was demonstrated. The enzyme-decorated OMVs provided synergistic cellulose hydrolysis resulting in 23-fold enhancement in glucose production than free enzymes.
Three-Photon fluorescence imaging deep inside scattering media

Investigating the complex structure and functioning of biological systems through light microscopy represents a foundational pillar of the life sciences. However, the very complexity of the samples cellular structure leads to one of the main limitations of optical microscopy: light scattering confines it to the outer most tissue layers. In the past decades, multiphoton microscopy successfully extended the accessible depth ranges and on a different front, advances in optical wavefront shaping showed that scattering can be compensated for, even in regimes where light fully lost its initial directionality. The aim of this action is to establish scattering correction through wavefront shaping in higher-order multiphoton microscopy, namely three-photon fluorescence imaging, to surpass the scattering barrier and reach unprecedented depths in optical microscopy.
Endovascular trophoblast and preeclampsia: A reassessment

Since the earliest report on impaired spiral artery remodelling in preeclamptic human pregnancies, numerous studies have been devoted to possible mechanisms of impaired trophoblast invasion. A better knowledge of early uteroplacental blood flow has provided a physiological context for the processes of spiral artery invasion and associated remodelling, revealing a closely timed relationship between increasing flow and early steps in vascular remodelling. Concerning the impaired trophoblast invasion in preeclampsia, it has also to be considered that impaired invasion not only concerns invasion depth per se, but also the extension of this deep invasion from the central towards the more lateral spiral arteries of the placental bed. Since also in preeclampsia the very central spiral arteries may be normally invaded, the existence of such spatial gradient provides a further dimension to the problem. A practical consequence is that frequently used rodent models, which show invasion of two or three spiral arteries only, may be less useful for studying this particular aspect of the disease. Amongst non-human primates, baboons and rhesus monkeys are 'shallow invaders', and only in some of the great apes deep trophoblast invasion and associated spiral artery remodelling occurs. A better knowledge of the evolutionary history of deep invasion and its possible selective benefit might ultimately improve our understanding of failed deep invasion and impaired spiral artery remodelling in preeclampsia.
Microsatellite radar network for fast update arctic ice surveillance.

Companies involved in Artic operations have a common need for fast update ice surveillance imagery to provide quick response to the risks posed by moving ice fields for vessels moving at several knots. Right now satellite picture providers offer response times of up to 16 hours with a 12-hour refresh time, creating a need directly confirmed by potential users for systems that offer a higher picture refresh rate. Bringing together expertise in military radars, microsatellites and data analysis, our team has developed an ice information service that offers a 2-hour image refresh rate for tracking ice features using a constellation of 6 microsatellites with Synthetic Aperture Radar (SAR) imaging. The ICEYE solution will provide customers up to 25% increase in operational efficiency, reducing fleet sizes and greatly lowering the risk of tanker accidents in the Arctic.
The need has been confirmed by our market studies in the case of oil exploration and the offshore supply market for permanent rig and support operations. Our customer development activities with potential clients show an important number of planned Arctic operations within the next few years, driving their interest and willingness to pay for the ICEYE information services. The spectrum of potential customers also includes industries not related to the Arctic, such as insurance, crisis monitoring and response, defence and crop monitoring in line with the EU Copernicus programme. As an entrepreneurial spinoff from a university business and engineering project, Iceye Arctic Intel is an innovation-driven company whose size has not been an obstacle to attracting the interest of major players with a key stake in our growth to become a commercial service provider with the launch of our first satellite. Thus the objective of this project is to integrate, pilot test and demonstrate the ICEYE system in its final form as a microsatellite in order to attract private investment for launching.
Additional file 2 of Characterization of the prevalence of excess weight in Brazil

Additional file 2.