Enhancing performance in numerical magnitude processing and mental arithmetic using transcranial Direct Current Stimulation (tDCS)

Frontiers in Human Neuroscience, 7

section_content: The relation between mathematical functions and the brain has fascinated neuroscientists since centuries. As one of the first modern neuroscientists, Rudolph Wagner studied the brain of the mathematical genius Carl Friedrich Gauss in the nineteenth century (Hagner, 2004). Over the last decades, a growing body of neuroimaging research led to a steadily increasing understanding of mathematical functions in the brain. The main focus of this research was on basic number processing, in particular on how numerical magnitudes are represented and processed in the brain. These questions were mainly addressed by administering number comparison tasks in which participants are presented with two numerical magnitudes and have to decide which of the two magnitudes is larger. Already Moyer and Landauer (1967) employed this task and found that reaction times and error rates were inversely related to the numerical distance between the two magnitudes. For instance, discriminating between the numbers 8 and 9 takes longer and is more error-prone than discriminating between 2 and 7. This distance effect has been replicated several times and is assumed to derive from the nature of the internal magnitude representation, which is often modeled as activation di s t ri but i o n so nano ri e n t e dm e n t aln um be rl i n e ( Nieder, 2005; Nieder and Dehaene, 2009). Importantly, functional magnetic resonance imaging (fMRI) studies have observed negative correlations between numerical distance and the activation of the intraparietal sulcus (IPS), a brain region in the posterior parietal cortex (PPC; e. g., Pinel et al., 2001; Fias et al., 2003). Since this brain region has also been found to be systematically involved in other tasks that require the processing of numerical magnitudes (e. g., approximation), there is now wide consensus that the IPS holds an amodal semantic representation of numerical magnitudes (Dehaene et al., 2003; Ansari, 2007 Ansari,, 2008)). In most neuroimaging studies, the PPC of both hemispheres is equally activated in numerical magnitude processing (Arsalidou and Taylor, 2011). Studies with split-brain patients confirm these studies by showing that both hemispheres are able to process numerosities independently (Seymour et al., 1994). 

Building on the findings from basic number processing research, there is now increasing interest into more complex mathematical functions, such as mental arithmetic. Mental arithmetic contains operations which are usually taught in school, such as addition, subtraction, multiplication, or division. Solving arithmetic problems is typically accompanied by activation in a widespread network of brain regions. The composition of these regions seems to depend on the operation, problem size and the applied problem solving strategy (Grabner et al., 2009). Simple one-digit multiplications, for example, are most often solved by retrieving the solution from memory (fact retrieval), which strongly relies on the angular gyrus. Complex subtraction problems, in contrast, require procedural strategies (e. g., decomposing the problem and calculation) which strongly draw on PPC regions including the IPS. In addition, the lateralization of PPC activity also seems to depend on these factors. In a meta-analysis by Arsalidou and Taylor (2011), the authors found that in addition and partially also in subtraction tasks, the left hemisphere was more strongly involved, while multiplications were processed primarily in the right hemisphere. 

One main disadvantage of human neuroimaging methods, such as fMRI, is their correlative nature. These methods indeed allow describing cortical regions which are activated during certain cognitive tasks, the causative role of these regions, however, remains unknown. Therefore, several studies investigated the effect of transcranial magnetic stimulation (TMS)-which allows to transiently inhibit cortical areas-on mathematical processing [for a review, cf. Sandrini and Rusconi (2009) ]. Most TMS studies investigated the effect of PPC-inhibition on numerical magnitude tasks. The majority of these studies have reported an impairment in number comparison performance after left-sided inhibition (Göbel et al., 2001; Sandrini et al., 2004; Andres et al., 2005; Rusconi et al., 2005; Knops et al., 2006; Cappelletti et al., 2007), whereas the effect of a right-sided disruption is less consistent (Göbel et al., 2001; Sandrini et al., 2004; Andres et al., 2005; Cappelletti et al., 2007; Cohen Kadosh et al., 2007). To our knowledge, only two TMS studies investigated mental arithmetic processing. Göbel et al. (2006) inhibited the PPC during a complex double-digit addition task and were able to induce transient impairments after left-, but not after right-sided inhibition. A recent study by Andres et al. (2011) compared performance in subtraction and multiplication tasks and found increased latencies for both arithmetic operations after inhibition of the IPS in both hemispheres. 

TMS is not only used to determine the causative role of cortical areas in various cognitive domains, but is also applied as a treatment in a wide variety of neurologic and neuropsychiatric disorders (Croarkin et al., 2011; Holtzheimer et al., 2012; Peng et al., 2012). However, due to the high costs of TMS and because of safety risks when enhancing cortical regions (Hummel and Cohen, 2006; Cohen Kadosh et al., 2012), a related method, namely transcranial Direct Current Stimulation (tDCS) has been increasingly used in recent years both in basic research and for treatment. TDCS modulates neural activity of cortical regions underneath the areas to which the electrodes are attached (Im et al., 2008). While anodal tDCS enhances the neural firing rate of the cortex under the anode, cathodal tDCS lowers the spiking rates beneath the cathode (Bindman et al., 1964). TDCS has been shown to be effective in modulating a variety of cognitive functions, such as working memory, pitch memory, verbal performance, or even driving behavior (e. g., Fregni et al., 2005; Vines et al., 2006b; Beeli et al., 2008a,b; Cerruti and Schlaug, 2008). Moreover, it has been shown to be successful as a remediation program for several neurologic and neuropsychiatric disorders (e. g., Boggio et al., 2008; Monti et al., 2008; Schlaug et al., 2008; Nitsche et al., 2009; Lindenberg et al., 2010). 

An interesting novel application field of tDCS could be the treatment of individuals suffering from mathematical learning disorders such as developmental dyscalculia (DD). With a prevalence of 5% (Cohen Kadosh and Walsh, 2007), DD is a frequent developmental disorder with impairments in magnitude processing (Landerl et al., 2004) and mental arithmetic (Geary, 1993 (Geary,, 2010)). If anodal tDCS could improve these impaired mathematical functions similar to the aforementioned (domaingeneral) cognitive processes, it undoubtedly has a large potential for the development of remediation programs for DD (Cohen Kadosh et al., 2012). Therefore, there is an urgent need for studies investigating effects of tDCS on mathematical functions. 

So far, however, only one study investigated the potential beneficial effect of anodal tDCS in the domain of mathematics. Cohen Kadosh et al. ( 2010 ) investigated whether tDCS can support the acquisition of new number symbols. The participants performed a number comparison task with new number symbols while they were stimulated. The authors found that learning was enhanced applying right-sided, but not left-sided stimulation. However, due to the small sample size used in this study, the generalizability of the obtained finding can be questioned. 

Theaimofthepresentstudywastoevaluatebeneficialeffects of tDCS on both mathematical functions that are essential in the development of mathematical competence and which are also frequently impaired in DD: numerical magnitude processing and mental arithmetic. We sought to evaluate whether tDCS can enhance performance in these domains under the consideration of the unclear hemispheric lateralization. Similar to previous neuroimaging and TMS studies, we administered a number comparison task to assess effects on magnitude processing. For investigating mental arithmetic, we required participants to solve double-digit subtraction problems which strongly rely on procedural strategies. Both tasks were administered before and after different tDCS protocols in two experiments. In the first experiment, we applied anodal tDCS over the left PPC (LA) as well as over the bilateral PPC (BA). This procedure was chosen as the lateralization of the assessed tasks is not completely clear based on existing evidence. On the one hand, neuroimaging studies suggest a bilateral involvement of the PPC in magnitude processing with a lateralization trend toward the left hemisphere for subtraction problems (Arsalidou and Taylor, 2011). On the other hand, the majority of TMS studies reported stronger effects for left-sided stimulation in both tasks. To additionally test whether inhibitory tDCS has similar effects as TMS, we also assessed the effect of bilateral cathodal stimulation (BC). The second experiment was motivated by the recent study of Cohen Kadosh et al. (2010) who reported beneficial effects of tDCS over the right PPC. Consequently, we additionally tested the effect of anodal stimulation of the right PPC (RA) in both tasks. In both experiments, we applied a within-subjects design and compared the stimulation protocols with a sham (placebo) condition (S). 

For the numerical magnitude processing task, we hypothesized that BA stimulation leads to performance improvements, whereas BC stimulation results in a performance decrease. We assumed that one-sided anodal stimulations (LA, RA) would result in an intermediate effect, between BA and S. For the complex subtraction task, we hypothesized that LA as well as BA would lead to improvements, because subtraction has been associated with left-hemispheric or bilateral activation. Furthermore, we speculated that BC inhibition might lead to a performance decrease.
section_name: INTRODUCTION

section_name: MATERIALS AND METHODS EXPERIMENT 1

section_content: In Experiment 1, the sample consisted of 21 right-handed participants (11 females) with a mean age of 22. 8 years (±3. 1). The subjects were recruited from the local universities and did neither have any history of neurologic or psychiatric disorders nor of DD or other mathematical disabilities. One participant had to be excluded prior to analysis due to deviant behavior during stimulation (making phone calls instead of reading silently). The local ethic committee (ethic committee of the canton of Zurich, Switzerland) approved the study, and each participant gave written informed consent.
section_name: Participants

section_content: The subjects participated in four sessions with a minimum intersession interval of 24 h (Figure 1A ; time between two sessions: 5. 3 d ± 5. 0). Each session took approximately 1 h. All sessions followed the same procedure: before and after the tDCS stimulation, subjects had to solve the computerized tasks (number comparison, subtraction), presented on a 17 ′′ computer screen using Presentation (13. 0, Neurobehavioral Systems, Albany, USA). In between, tDCS was applied. A different stimulation protocol was used for each session. We Task 1: Double-digit number comparison: To e v a l u a te numerical magnitude processing, we used a number comparison task (Figure 2A ) similar to previous TMS studies (Göbel et al., 2001; Cappelletti et al., 2007). The subjects had to judge as fast as possible whether the presented Arabic number was bigger or smaller than 65. We used numbers from 31-99 (except 65), each presented twice. Between the stimuli, a fixation cross was presented for 250 ms. 

Task 2: Double-digit subtraction task: To e v a l u a te t D C Seffects on procedural mental arithmetic, a task containing 50 two-digit subtractions (Figure 2B ) was presented. All subtractions were decade-overlapping and resulted in two-digit numbers. First, the subjects had to calculate the presented subtraction as fast as possible and indicate that they found the solution by pressing "0" on the number pad of the keyboard. Subsequently, three result options were displayed and the subjects had to indicate the correct solution by pressing "1", "2", or "3" on the number pad. Each time, 2 out of 6 distractors were randomly chosen and displayed together with the correct solution in a random order. The distractors were generated as follows: result ±1, ±2, and ±10 to prevent guessing. Between the calculations, a fixation cross was presented for 250 ms. Similar tasks have already been used in other fMRI (e. g., Ischebeck et al., 2006), but not in brain stimulation studies. The latency between stimulus presentation and first key press was analyzed as reaction time. We decided to do a two-step task rather than presenting possible solutions simultaneously with the subtraction in order to prevent participants from simply approximating the subtraction rather than computing the exact result.
section_name: Experimental design

section_content: A DC-Stimulator distributed by NeuroConn GmbH (Ilmenau, Germany) was used as constant direct current source. The current was applied on the head surface using rubber electrodes, covered with saline-soaked sponges. TheactiveelectrodewasplacedoverP3and/orP4oftheinternational EEG 10-20-system (Jasper, 1958), which corresponds with the PPC centering at the IPS (Herwig et al., 2003). The reference electrode was placed over the contralateral supraorbital region. The combination "parietal lobe-contralateral supraorbital region" was already successfully used in other tDCS-studies (e. g., Vines et al., 2006b). A current density of 0. 029 mA/cm 2 (active electrode: 35 cm 2, 1 mA) was applied for 25 minutes, which resulted in a total charge density of 428. 6 C/m 2. Thesizeof the reference electrode was 100 cm 2 and therefore without influence to the underlying brain region (Nitsche et al., 2007). The current was ramped up and down for 8 s. 

We used four different stimulation conditions: Bilateral anodal tDCS (BA): In our setup, which we will refer to as bilateral tDCS, we attached two active electrodes over P3 and P4, respectively. The two reference electrodes were placed at the contralateral supraorbital areas. 

With bilateral tDCS, we assume similar mechanisms to take effect as in traditional tDCS (one active, one reference electrode). This assumption is based on simulation studies showing that such a setup has approximately similar effects as traditional tDCS with respect to its focality and the current density distribution (Miranda et al., 2006). However, as in traditional tDCS-setups, the precise location of the maximum current density is not completely clear. Recent advances in modeling suggest that tDCS effects also depend on the tissue properties and individual anatomical differences (Salvador et al., 2010; Dmochowski et al., 2011; Neuling et al., 2012). We applied the bilateral anodal setup to maximize potential beneficial effects of tDCS given the assumption that both PPC are essential in mental arithmetic and in numerical magnitude processing Left-hemispheric anodal tDCS (LA): We used the same setup as in BA. In contrast to BA, only the left tDCS-device (active electrode at P3, reference over right eyebrow) was turned on. The second tDCS-device was placed without applying any current, in order to blind the subjects with respect to the applied stimulation protocol.
section_name: Transcranial Direct Current Stimulation (tDCS)

section_content: The electrode configuration was the same as in BA. We applied cathodal stimulation on P3 and P4 to inhibit both PPC in order to replicate previous TMS-induced impairments in numerical magnitude processing (Andres et al., 2005). 

Sham stimulation (S): Sham stimulation was applied using the BA setup. In contrast to the active stimulation sessions, we applied current for only 30 s. This placebo-condition is known to be indistinguishable by the participants (Gandiga et al., 2006).
section_name: Bilateral cathodal tDCS (BC):

section_content: The statistical analysis was conducted using SPSS 19 (IBM Corp., Armonk, USA). As dependent variable, we used the stimulation induced changes in accuracy and response latencies (post-pre). Therewith, we controlled for offline-training effects and other constitutional biases. The data were analyzed using repeatedmeasures ANOVAs with the within-subject factor "stimulation" (LA, BA, BC, S). For significant effects, we computed post-hoc paired t-tests for the active stimulations vs. sham. The results were, if not indicated differently, Bonferroni corrected for multiple comparisons.
section_name: Statistical analysis

section_name: EXPERIMENT 2

section_content: Sixteen healthy, right-handed subjects (9 females) aged 23. 6 years (±2. 4) participated in Experiment 2. For the participants in the second experiment, the same inclusion criteria as in Experiment 1 were applied (see above). The participants in both experiments did not differ in their age [t (34) =-0. 72, p = 0. 479] or intellectual abilities [t (34) = 0. 42, p = 0. 677] as assessed by the KAI (Kurztest für allgemeine Basisgrössen der Infomationsverarbeitung; Lehrl et al., 1992).
section_name: Participants

section_content: The subjects attended two sessions which were at least 24 h apart (Figure 1B ; time between the sessions: 4. 4 d ± 2. 9). The protocol was the same as in the first experiment with exception of the stimulation: the subjects were stimulated with right anodal (RA) or sham (S) stimulation. The tasks as well as the stimulation duration/intensity were similar as in Experiment 1. For the stimulation, we used only one standard tDCS-device. 

Right-hemispheric anodal tDCS (RA): The anodal electrode was placed at P4 of the 10-20 system and the reference electrode was attached to the left supraorbital region. 

Sham stimulation (S): For sham stimulation, we used a similar setup as in RA, with the same sham stimulation parameters as in Experiment 1.
section_name: Experimental design

section_content: The statistical analysis was conducted using SPSS 19 (IBM Corp., Armonk, USA). To evaluate the tDCS-induced effects on performance, we compared the changes in accuracy and response latencies between the active (RA) and sham stimulation (S) using paired t-tests.
section_name: Statistical analysis

section_name: RESULTS EXPERIMENT 1

section_content: The analysis of the reaction times (Ta b l e 1 )d i dn o tr e v e a la n y significant stimulation effects [F (3, 57) = 1. 41, p = 0. 25]. 

Regarding the accuracy (Ta b l e 1 ), we found a significant difference between the stimulation conditions [F (3, 57) = 4. 32, p = 0. 008]. Post-hoc t-tests revealed a significant effect in LA [t (19) = 3. 47, p = 0. 009, Figure 3 ], but not in BA [t (19) = 1. 30, p = 0. 627] or BC [t (19) = 2. 30, p = 0. 099] compared to S. To validate that these differences are not due to differences in the pretest, we additionally compared the accuracies before the stimulation, where we did not find any significant difference [F (3, 57) = 1. 08, p = 0. 364]. 

To evaluate the reliability of our data, we also tested whether we can replicate the distance effect (Moyer and Landauer, 1967). We split the trials into far (31) (32) (33) (34) (35) (36) (37) (38) (39) (40) (41) (42) (43) (44) (45) (46) (47) (48) (49) (50) (80) (81) (82) (83) (84) (85) (86) (87) (88) (89) (90) (91) (92) (93) (94) (95) (96) (97) (98) (99) and close (51-79), similar as in Cappelletti et al. (2007) We also tested whether the distance effect was influenced by the stimulation condition. A repeated measures ANOVA of the differences between close and far trials between pre and post sessions [e. g., S = (S_far post -S _ c l o s e post )-( S _ f a r pre -S_close pre )] did not reveal any significant stimulation effect in reaction time [F (3, 57) = 0. 46, p = 0. 714] or accuracy [F (3, 57) = 2. 55, p = 0. 065].
section_name: Number comparison

section_content: In the reaction times (Ta b l e 1 ), we found a significant effect of stimulation [F (3, 57) = 3. 01, p = 0. 037, Figure 5 ]. Post-hoc ttests showed a significant improvement after LA [t (19) = 2. 38, p = 0. 048, one-tailed], but not after BA [t (19) = 0. 77, p = 1. 00] or BC [t (19) = 0. 69, p = 1. 00] compared to S. To ensure that these differences are not due to any differences in the pre-test, we additionally compared the mean reaction times before the stimulation, where we did not find any significant difference [F (3, 57) = 1. 43, p = 0. 243]. 

All subjects solved the subtractions very accurately (Ta b l e 1 ). The stimulations had no influence on the accuracy [F (3, 57) = 0. 57, p = 0. 634]. Also in this experiment, we found the distance effect for all conditions in reaction time [S pre : t (15) = 5. 56, p < 0. 001, all uncorrected; S post : t (15) = 4. 48, p < 0. 001; RA pre : t (15) = 5. 24, p < 0. 001; RA post : t (15) = 8. 66, p < 0. 001] and accuracy [S pre : t (15) = 3. 77, p = 0. 002, all uncorrected; S post : t (15) = 5. 65, p < 0. 001; RA pre : t (15) = 4. 39, p = 0. 001; RA post : t (15) = 4. 36, p = 0. 001]. There was no stimulation induced change in the distance effect for reaction times [t (15) = 0. 95, p = 0. 359] or accuracy [t (15) = 0. 29, p = 0. 776].
section_name: Subtraction

section_content: The reaction times did not change significantly due to the stimulation [t (15) = 1. 13, p = 0. 275]. Also the accuracy did not differ significantly [t (15) = 0. 07, p = 0. 944].
section_name: Subtraction

section_content: In our daily life, it is essential to be able to quickly and correctly access numerosities and perform more complex numerical manipulations, such as solving subtractions. Impairments of these functions, such as in DD, lead to huge challenges during schooling and professional career (Bynner and Parsons, 1997; Parsons and Bynner, 2005). In recent years, tDCS (Nitsche and Paulus, 2000) has been introduced as a promising tool to enhance cognitive functions (Cohen Kadosh et al., 2012). In this study, we therefore evaluated whether tDCS could be used to improve performance in number comparison and mental arithmetic. A first indication of a beneficial effect of tDCS in learning a new number-symbol system has been presented by Cohen Kadosh et al. (2010). The authors found improvements in the acquisition of new numerical symbols in a small sample of healthy adults after right-sided anodal stimulation of the PPC. However, other functions which are important for mathematics, such as numerical magnitude processing or mental arithmetic, have not yet been studied. 

In the present study, we found that subjects responded significantly more accurately after left-sided anodal stimulation (LA) of the PPC compared to sham stimulation in a number comparison task. Furthermore, LA also induced performance improvements compared to sham in a complex subtraction task. These findings provide the first demonstration that tDCS might be helpful for improving mathematical performance in several domains. Due to the unclear lateralization of mental arithmetic and numerical magnitude processing and the unclear interaction of both hemispheres, we also investigated the effects of simultaneous bilateral stimulation using two tDCS devices. Similar setups have been successfully administered in previous studies (e. g., Vines et al., 2008; Lindenberg et al., 2010). Our hypothesis that the simultaneous stimulation of both hemispheres would lead to more pronounced effects could, however, not be supported. We did not find any significant change in performance in both, the excitation (BA) as well as the inhibition (BC) condition. This suggests that the interaction between the two hemispheres might be more complex than, for instance, merely additive. For instance, it may be organized according to the interhemispheric inhibition (IHI) principle (Ferbert et al., 1992). IHI is well known from motor cortices (Vines et al., 2006a), and has already been suggested for the PPC in visual processing (Battelli et al., 2009). One could therefore speculate that the left PPC, which turned out to be crucially involved in the tested functions, is inhibited by the right PPC, which could be less important in the tested functions. A bilateral excitation would therefore annihilate the beneficial effect of LA. However, such an interaction in the domain of mathematics must be explored in more depth. 

In our second experiment, we did not find any changes in performance after right-sided anodal PPC stimulation in number comparison or subtraction. Our findings therefore seem to contradict the findings by Cohen Kadosh et al. (2010). However, it should be noted that our study design was different in several aspects. First, our study investigated the effects of brain stimulation on the performance of mathematical tasks, whereas Cohen Kadosh et al. ( 2010 ) investigated the learning of numbersymbol associations over a period of several days. Second, we used a different stimulation protocol. Cohen Kadosh et al. ( 2010 ) stimulated while the participants learned symbol-numerosityassociations. We used an offline-tDCS protocol where participants performed the tasks before and after the stimulation. Finally, we assessed different aspects of mathematics. Cohen Kadosh et al. (2010) interested in the learning of symbolnumerosity-associations, whereas we investigated magnitude processing and mental arithmetic. 

Our finding that only LA improves performance in numerical magnitude processing is in line with the majority of the TMS studies which found an impairment after left-sided inhibition (Göbel et al., 2001; Sandrini et al., 2004; Andres et al., 2005; Rusconi et al., 2005; Knops et al., 2006; Cappelletti et al., 2007; Dormal et al., 2008). TMS studies, which investigated right-sided inhibition, on the other hand, led to inconsistent results (Göbel et al., 2001; Sandrini et al., 2004; Andres et al., 2005; Cappelletti et al., 2007; Cohen Kadosh et al., 2007; Dormal et al., 2008). 

Likewise, our finding that the left PPC is crucially involved in subtractions is in line with fMRI-studies which usually show a slightly left-lateralized activation pattern (Arsalidou and Taylor, 2011). Since no previous brain stimulation study has shown a beneficial effect of brain stimulation on subtractions, our findings crucially further the understanding of the PPC during complex subtractions. 

Although we have convincing evidence that LA improves numerical magnitude processing and complex mental arithmetic, the present study has one minor limitation. Because we wanted to avoid that our results might be confounded by effects which are not related to tDCS or by individual differences, such as mathematical competence which is known to influence neural processes (Grabner et al., 2007), we decided to conduct a withinsubjects design. The participants therefore attended four sessions in Experiment 1. To ensure that training effects do not have any influence on the results, we counter-balanced the stimulation conditions across sessions. Furthermore, we compared performance changes after the stimulation to the baseline performance, assessed before stimulation in every session. 

The present findings encourage a further exploration of the beneficial effects of left anodal tDCS at the PPC in the domain of mathematics. Although we have shown its beneficial effect on magnitude processing and mental arithmetic, the effect of tDCS on several aspects of mathematics still needs to be evaluated. In the domain of mental arithmetic, it should be tested whether LA also has beneficial effects on operations and strategies other than the procedures we tested in this study. For example, it should be tested whether small additions, which are usually retrieved from memory, also benefit from LA. Furthermore, the effect of tDCS on the acquisition of arithmetic skills, such as the learning of arithmetic facts and strategies, should also be evaluated, because these are key competencies taught in school. Not only shouldtheeffectsofLAbetestedbehaviorally,butalsotheneural mechanism underlying these effects should be studied using neuroimaging methods such as electroencephalography and fMRI. If such studies reinforce the usefulness of LA, it has to be assessed whether these beneficial effects also improve performance in people with mathematical disabilities, such as DD or acalculia, where remediation is still sparse (Cohen Kadosh and Walsh, 2007).
section_name: DISCUSSION

section_name: ACKNOWLEDGMENTS

section_content: We thank S. Studer for her help collecting the data and B. Rütsche for helpful comments. Tobias U. Hauser and Roland H. Grabner are supported by the Swiss National Science Foundation.
section_name: ACKNOWLEDGMENTS

section_name: Conflict of Interest Statement:

section_content: The authors declare that the research
section_name: Conflict of Interest Statement: