Corrigendum: The lateral superior olive in the mouse: Two systems of projecting neurons


section_content: The lateral superior olive in the mouse: Two systems of projecting neurons by Williams, I. R., Filimontseva, A., Connelly, C. J., and Ryugo, D. K. (

). Front. Neural Circuits :

. doi:. /fncir. . 

In the published article, there was an error in the legend for Figures: Figure 1, Figure 2, Figure 3, Figure 4, Figure 5, Figure 6, Figure 7, Figure 8, Supplementary Figure 5. All figures contain the statement below that should have only been included for Supplementary Figure 5 :

"The image approval for our adaptation of Stiebler and Ehret (1985) from John Wiley and Sons via the Copyright Clearance Centre; license number 5402831110296. "

The corrected legends appear below: Gray arrowheads: PO neurons on the borders of the LSO. (C) Photomicrographs (100x objective) of the ipsilateral (top row) and contralateral (middle row) principal cells labeled from chromogenic development of FG with DAB (brown) or NiDAB (black) and fluorescent tracers (FD-green, FG-yellow, Antionia Red-magenta). The principal neurons were fusiform with unipolar or bipolar dendritic extensions. The preolivary neurons were also labeled (bottom row) and featured a large, polygonal cell body using chromogenic development. ipsi., ipsilateral; contra., contralateral; FG, fluorogold; FD, fluorescein dextran. Scale bar equals 100 µm (A,B), 25 µm (C). 

Figure 2. Labeled lateral olivocochlear (LOC) efferents following retrograde tracer injections into the cochlea. (A) Schematic illustration of retrograde tracer injected through the round window of the left cochlea. Solid yellow line indicates the primary pathway to the ipsilateral LOCs, whereas the dashed line leads to the very few contralateral LOCs. Gray arrowheads: shell neurons on the borders of the LSO. (B) Photomicrograph (100x objective) of the intrinsic LOC efferents (top row) labeled with chromogenic development (DAB-brown, NiDAB-black) and with fluorescent markers (FG-yellow, FD-green). The intrinsic neurons were small and round, and look similar to the principal neurons. The shell neurons (bottom row) were labeled in the same tissue as the intrinsic neurons, and featured a large cell body with broader dendritic extensions. ipsi., ipsilateral; contra., contralateral; FG, fluorogold; FD, fluorescein dextran. Scale bar equals 100 µm (A), 25 µm (B). Figure 5. Topographic relationship between the IC and the LSO. Tracing of IC injection sites progressing more ventral and higher in frequency [(A-D), left column]. The corresponding bilateral labeling of LSO principal cells (ipsilateral-dark blue, contralateral-dark red, middle two columns) and periolivary (PO) cells (ipsilateral-light blue, contralateral-light red) were traced through serial sections, aligned using blood vessels, and merged. The ipsilateral and contralateral labeling for each injection was combined (last row) to show the bilateral preservation of topographic and tonotopic organization of principal cells across the LSO. PO cells do not observe a tonotopic distribution (light blue and red). kHz, kilohertz. 

Figure 6. Tonotopic relationship between the IC and LSO with a tonotopic axis. (A) Several IC injections were made in the dorsal to ventral regions of the CNIC to labeled the correspondingly low to high frequency output in the LSO. 8 IC injection sites were drawn and superimposed to present an IC frequency representation. Frequencies included: 8, 11, 20, 22, 33, 47, 55, and 60 kHz (color coded). (B) The corresponding label of principal neurons in the LSO, color coded to match. The labeled principal cells show the LSO low to high frequency organization progressing from the lateral to medial limb, respectively. (C) The corresponding LSO neurons labeled from each IC injection were color coded. A region for each color of principal cells representing one frequency was drawn by outline the area of labeled cells to form a lamina for each frequency. A line representing the long axis of each frequency region was drawn to represent an isofrequency line. (D) A line (black) representing the tonotopic axis was drawn by connecting centroids of all the isofrequency lines and was compared to other tonotopic axis lines (red) derived from the Hamilton Jacobi output from other LSO sections. A color map is included, with purple corresponding to labeled elements from 8 kHz, and red corresponding to the highest frequency elements of 60 kHz. IC, inferior colliculus; LSO, lateral superior olive; kHz, kilohertz. 

Figure 7. Plot of the angles for LSO neurons with ascending (red) or descending (black) projecting axons with respect to the tonotopic axis. Angle measurements for the four types of LSO neurons are shown and combined from three regions of the LSO (rostral, middle, and caudal sections). The average angle (black line) was presented for all subtypes. Gray dashed line: 45-degree threshold. Principal and intrinsic neurons illustrate similar angle deviations, with mean alignment below the 45-degree threshold across all planes. Periolivary (PO) neurons and shell efferent neurons both had values above the 45-degree threshold for all
section_name: A corrigendum on

section_name: Publisher's note

section_content: planes. Smaller values indicate alignment with the frequency organization. PO, periolivary. LSO neurons with ascending or descending projections labeled from separate LSO sections were superimposed and collapsed as one color (black) on a representative rostral, middle, and caudal LSO section. The somatic long axis line (red) is contrasted against the individual neurons. (E) A model LSO representing rostral, middle, and caudal sections was drawn (gray, fourth row). Here, the relative alignment of principal and intrinsic neurons are shown with the representative tonotopic axis (black dashed line). An overall organization within the nucleus is seen to comprise fibrodendritic laminae. PO, periolivary; LOC, lateral olivocochlear. 

Supplementary Figure 5. Comparison of anatomically derived isofrequency layers (left) and electrophysiologically derived isofrequency lines (right). The anatomical laminae represent a collection of reconstructed profiles of chromogenically-recovered injection sites at their midpoint from CBA/CaH mice. Profiles are color coded with respect to the frequency spectrum (lower left). The isofrequency lines were taken from Figure 7B (Stiebler and Ehret, 1985) where they connected points of equivalent frequency as recorded in the IC of the house mouse. We color coded their map to the frequency spectrum. The tonotopic organization for the IC is remarkably consistent given the different methods, histology, mouse strains, and eras. kHz, kilohertz. The image approval for our adaptation of Stiebler and Ehret (1985) from John Wiley and Sons via the Copyright Clearance Centre; license number 5402831110296. 

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

section_content: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
section_name: Publisher's note