Supplementary Figures 1-5 from Extensive Promoter DNA Hypermethylation and Hypomethylation Is Associated with Aberrant MicroRNA Expression in Chronic Lymphocytic Leukemia

<jats:p>&lt;p&gt;PDF file - 90K, Supplementary Figure S1 contains technical validations of differentially methylated miRNA promoters by high resolution quantitative DNA methylation analysis. Supplementary Figure S2 shows experimental validation of promoter activity for the mir-129-2 locus. Supplementary Figure S3 shows relative expression of predicted miRNA target genes by qPCR in CLL specimens and healthy B cell controls. Supplementary Figure S4 shows an unsupervised clustering of quantitative DNA methylation data at the identified hyper- and hypomethylated miRNA promoters in CLL specimens, healthy B cell samples, various normal hematopoietic cells and several solid tissues. Supplementary Figure S5 shows a detailed quantitative DNA methylation analysis of the miR-155 host gene 5' region and correlations with miR-155 expression in CLL specimens, healthy B cell samples, various normal hematopoietic cells and several solid tissues&lt;/p&gt;</jats:p>


section_content: Results are depicted as heatmap (yellow, unmethylated; blue, highly methylated). (C) Average DNA methylation over the depicted regions is correlated with expression as measured by qPCR in the respective tissue relative to the average expression of the three houskeeping RNAs SCARNA17, SNORD25 and SNORA73A. For CLL, healthy B and T cells as well as granulocytes, the average of all respective samples is used.