Two oppositely-charged sf3b1 mutations cause defective development, impaired immune response, and aberrant selection of intronic branch sites in Drosophila

<jats:p>SF3B1 mutations occur in many cancers, and the highly conserved His662 residue is one of the hotspot mutation sites. To address effects on splicing and development, we constructed strains carrying point mutations at the corresponding residue His698 in <jats:italic>Drosophila</jats:italic> using the CRISPR-Cas9 technique. Two mutations, <jats:italic>H698D</jats:italic> and <jats:italic>H698R</jats:italic>, were selected due to their frequent presence in patients and notable opposite charges. Both the <jats:italic>sf3b1-H698D</jats:italic> and–<jats:italic>H698R</jats:italic> mutant flies exhibit developmental defects, including less egg-laying, decreased hatching rates, delayed morphogenesis and shorter lifespans. Interestingly, the <jats:italic>H698D</jats:italic> mutant has decreased resistance to fungal infection, while the <jats:italic>H698R</jats:italic> mutant shows impaired climbing ability. Consistent with these phenotypes, further analysis of RNA-seq data finds altered expression of immune response genes and changed alternative splicing of muscle and neural-related genes in the two mutants, respectively. Expression of <jats:italic>Mef2-RB</jats:italic>, an isoform of <jats:italic>Mef2</jats:italic> gene that was downregulated due to splicing changes caused by <jats:italic>H698R</jats:italic>, partly rescues the climbing defects of the <jats:italic>sf3b1-H698R</jats:italic> mutant. Lariat sequencing reveals that the two <jats:italic>sf3b1-H698</jats:italic> mutations cause aberrant selection of multiple intronic branch sites, with the <jats:italic>H698R</jats:italic> mutant using far upstream branch sites in the changed alternative splicing events. This study provides in vivo evidence from <jats:italic>Drosophila</jats:italic> that elucidates how these SF3B1 hotspot mutations alter splicing and their consequences in development and in the immune system.</jats:p>

section_content: Pre-mRNA splicing, catalyzed by the spliceosome, a large and dynamic complex consisting of five snRNAs and >100 proteins, is critical for eukaryotic gene expression and regulation [Reviewed in 1, 2]. Human disease mutations in trans-acting splicing factor genes and cis-acting pre-mRNA sequences can alter or disrupt splicing and drive the development of cancers [Reviewed in 3, [4] [5] [6] [7] [8]. Mutations in nearly twenty splicing factors connected with cancers, are highly recurrent in myeloid malignancies [9] [10] [11] [12] [13] [14], chronic lymphocytic leukemia (CLL) [15] [16] [17] [18] and uveal melanoma (UVM) [19, 20], and also frequently occur in bladder carcinoma [21], breast cancers [22, 23], lung adenocarcinoma [21, 24] and pancreatic ductal adenocarcinoma [25]. 

Over the past decade, SF3B1, SRSF2, U2AF1 and ZRSR2, splicing factors that are involved in early intron selection and pre-spliceosome assembly, have been identified as the most frequently mutated splicing factors in cancers based on the fast-developing next-generation sequencing techniques [9, 10, 13, 16, 26]. Somatic mutations in SF3B1 are particularly prevalent in myelodysplastic syndromes (MDS) [9] [10] [11] 26] and CLL [16, 18], as well as in other solid tumors, such as UVM, pancreatic ductal adenocarcinomas and breast cancers [19, 20, 27]. 

SF3b is a 450-kDa hetero-heptameric protein complex and a major component of 17S U2 snRNP [28]. Studies in yeast and human have revealed that the SF3b complex is required for the formation of pre-spliceosomal complex A [29, 30] and directly interacts with the intronic branch site (BS) and flanking RNA sequences [31, 32]. After the U4/U6-U5 tri-snRNP joins in, the SF3b complex is released from the complex B act [33]. As the largest subunit of SF3b, SF3B1 has 20 highly conserved HEAT repeats in its C-terminus, and U2AF1/2-and SF3b14a-interacting motifs in its N-terminus [29, [34] [35] [36] [37]. SF3B1 also interacts with the splicing factors Prp5 [28, 38, 39], SUGP1 [40] and Prp3 [41]. 

Most of the SF3B1 mutations are located in its HEAT repeats, especially in HEATs 4-12, where five residues, R625, H662, K666, K700 and E902, are hotspots and most of them exhibit cancer lineage specificities [21, 42, 43]. Mutation K700E is linked with blood cancers [44], K666N is linked with AML [45], R625 mutations are linked with UVM [20, 27, 46], and E902 mutations are linked with bladder urothelial carcinoma (BLCA) [21]. However, H662 mutations have no obvious correlation with cancer-types, being found in MDS, AML, CLL, UVM and breast cancers [9, 10, [47] [48] [49] [50] [51] [52]. Recent cryo-EM structures have identified intermolecular hydrogen bonds between the intronic polypyrimidine tract and most of the hotspot residues in several spliceosomal complexes [53] [54] [55], and those mutations are also in the proximity of Prp5's highly-conserved DPLD motif in the human 17S U2 snRNP [28]. In addition, the SF3B1 mutations in blood cancers destabilize the SF3B1-SUGP1 interaction in humans [40], and their equivalent mutations in the yeast SF3B1-homolog Hsh155 lead to altered Hsh155-Prp5 interaction [38, 56]. 

Transcriptome analyses of human cell lines and mouse models have found that alternative 3 0 splice site (A3SS) events are enriched in SF3B1 mutation-mediated splicing changes, in which upstream cryptic 3 0 SSs are preferentially used [40, 47, [57] [58] [59]. Several mechanistic models have been proposed for this alteration: i) mutated SF3B1 facilitates selection of cryptic 3 0 SSs by either overcoming certain steric hindrance within a region downstream of BS [57], enhancing interactions of SF3B1 with specific nucleotides flanking the upstream BS [47] or increasing recognition of inaccessible 3 0 SSs buried in RNA secondary structures [58] ; ii) mutated SF3B1 induces a conformational change in the U2 snRNP complex leading to the selection of a stronger upstream BS [59] ; and iii) mutated SF3B1 disrupts its interaction with SUGP1 and facilitates BS recognition to use a cryptic 3 0 SS [40]. 

Sf3b1 mutations have also been studied for genetic interactions and for the sensitization of clinically relevant drugs, as well as the developmental and splicing defects in C. elegans [60]. In zebrafish, studies of Sf3b1 mutants have revealed that Sf3b1 is essential for the neural crest development [61] and the hematopoietic differentiation [62], and regulates erythroid maturation and proliferation via TGFβ signaling [63]. However, sf3b1 mutants in Drosophila have not been previously investigated. 

Several H662 mutations, including H662D and H662R, two oppositely-charged residues, have been found in multiple cancers without obvious type preference as mentioned above. However, it remains unclear how these H662 mutations alter splicing and whether they have in vivo effects on development. To test these mutations in an in vivo model system, we constructed two mutant fly strains, sf3b1-H698D and -H698R, in which the homologous residue His698 in Drosophila Sf3b1 was mutated to Aspartic acid (D) and Arginine (R), respectively. Both mutants exhibit defects in development; the H698D mutant has an impaired immune response to fungal infection, whereas the H698R mutant has a decreased ability in climbing. RNA-seq allowed us to find responsible candidate genes in each mutant, and lariat sequencing revealed that both mutations cause aberrant selection of branch sites and that the H698R mutant prefers to use upstream branch sites.
section_name: Introduction

section_name: Results

section_content: H662 mutations had been widely identified in patients as one of the five hotspots in SF3B1 (Fig 1A ). There may be additional mutational hotspots, but they have not yet been experimentally validated for their contribution to disease or splicing changes. We collected 34 available online reports in cancers and found five residue mutations of H662, including Q, D, R, Y, and N (S1 Table and refs therein ). This histidine residue is invariant in all species (Fig 1B). To address the mechanism of altered splicing by mutations of this highly conserved residue and their effects on development, we constructed Drosophila strains with mutations at H698, the corresponding residue of human H662, using the CRISPR-Cas9 system. Since our previous data in yeast demonstrated that the D and R mutations of Hsh155/SF3B1 result in opposite splicing effects on branch site mutant reporters and opposite effects on interaction with Prp5 [38], two mutant strains, sf3b1-H698D and -H698R, were constructed (Fig 1C ). The sf3b1-H698 homozygous mutants were successfully obtained and confirmed by genomic PCRs and Sanger sequencing (S1A Fig). In comparison to the WT strain, the mRNA and protein levels of sf3b1 and their cellular localizations were not detectably changed in the two mutant strains (Figs S1B and 1C).
section_name: Construction of sf3b1-H698 mutant flies by CRISPR-Cas9 system

section_content: To investigate the effects of sf3b1-H698 mutations on development, a variety of phenotypes were tested, including fecundity, hatching, pupation, eclosion and lifespan. In comparison to the WT, females of the two sf3b1 mutants laid significantly fewer eggs, roughly 35% fewer in the first 5-days (Figs 2A, S2A and S2B). Embryonic development of the two mutants was also impaired, showing 10-20% decreased hatching rates (Fig 2B ). The development of both mutants was obviously retarded in metamorphosis; both mutants exhibited less pupation and eclosion rates during the first 36 hours, H698D being worse than H698R (Fig 2C ). Furthermore, the lifespan of the two mutants was significantly shortened, going from the WT's median of 72 days to a median of 58 days (S2C Fig). Taken together, these phenotypes demonstrate that the H698 homozygous mutations in Sf3b1 result in defective development of Drosophila, with H698D being slightly more defective than H698R. We had preliminarily tested their heterozygous mutants, neither of which exhibited a notable phenotype; therefore, homozygous mutants were used for further investigations in this study.
section_name: sf3b1-H698 mutants are defective in development

section_content: When culturing fly strains, we noticed that the movement of the sf3b1-H698R mutant was obviously different from the other strains. Therefore, a climbing assay was performed for the adult flies [64]. Compared to the WT, the H698R adults exhibited a significantly decreased ability in climbing, which worsened when they were older. In contrast, the climbing ability of the H698D adults was not significantly changed for all four tested ages (Fig 2D ). 

We also investigated fungal infection of the mutants. After infection with Metarhizium anisopliae ARSEF 23 (Ma23), survival was significantly impaired for H698D females and males, but not significantly changed for H698R females and only slightly decreased for H698R males (Fig 2E ), suggesting that resistance to fungal infection is reduced in the H698D mutant. 

These results reveal that the two kinds of H698 mutations have different impacts on Drosophila movement and immune systems, suggesting that downstream genes of sf3b1, such as muscle, neuron or immunity-related genes, are affected differently by H698D and H698R mutations in Sf3b1.
section_name: H698R is defective in climbing, H698D is defective in immune response

section_content: We then performed mRNA-seq of the WT and mutant fly adults (5d), in which two lines of each sf3b1 mutant were sequenced for accuracy (S2 Table ). Correlation analyses of the two lines from each mutant strain suggested that they are highly consistent with each other (S3 Fig). Expression levels of 181 genes were significantly changed in both lines of the H698D mutant, and 120 genes were significantly changed in both lines of the H698R mutant; and 56 of them were shared in the H698D and H698R (Fig 3A and 3B and S3 Table). Further GO analyses indicated that genes in the mannose metabolic process and protein deglycosylation were highly enriched in the two mutants, whereas the innate immune response genes were enriched only in H698D (Fig 3C ). This is consistent with the above findings that both mutants are defective in development and that the H698D mutant is sensitive to fungal infection (Fig 2 ). 

To validate this, we performed RT-qPCRs to test levels of the innate immune response genes, including Cyp6a-17, Cyp12d-p, Cyp4e3 and PGRP-SC. The mRNA levels of Cyp4e3 and PGRP-SC were increased in both mutants, while those of Cyp6a-17 and Cyp12d-p were dramatically decreased in H698D but not in H698R (Fig 3D ). These data are consistent with the bioinformatic analyses, and suggest that the decreased fungal resistance of H698D would be due to the affected expression of cytochrome P450 (CYP) family genes that are involved in the detoxification of foreign compounds, such as the tested Cyp6a-17 and Cyp12d, both of which are known to have monooxygenase activity and are induced by xenobiotic treatment [65].
section_name: Innate immune response genes are affected in the sf3b1 mutants

section_content: Using the common differential AS (DS) analysis tool rMATS, we identified 1,149 and 1,290 AS events that were significantly changed in the two mutants, respectively (Figs 4A and S4, and S4 Table ). The most common belong to two groups, alternative 3 0 SS (A3SS) and 5 0 SS (A5SS); the other three groups (SE, skipped exon; RI, retained intron; MXE, mutually exclusive exons) were relatively less common. In each group, ~40-60% DS events were shared by the two mutants (Fig 4B). To validate this, we performed RT-PCRs of randomly picked events that are Decreased hatching rates of H698D and H698R mutants. Statistical differences were determined according to the t-test. The hatching rates of WT, sf3b1-H698D and -H698R are 89. 6%, 73. 3%, 82. 1%, respectively. The graph was shown by mean ± SEM, and p values were 0. 0452 for H698D and 0. 0211 for H698R. (C) Developmental stages were elongated for sf3b1 mutant strains. Time point started from the first day after egg-laying. (D) Decreased climbing ability of the sf3b1-H698R strain, but not the -H698D strain. Adults at four ages were assessed. Statistical data are shown as mean ± SEM, � : p < 0. 05, �� : p < 0. 01, ��� : p < 0. 001, ns: no significance. H698D-5d vs WT-5d: p > 0. 9999, H698R-5d vs WT-5d: p < 0. 0001, H698D-15d vs WT-15d: p = 0. 3401, H698R-15d vs WT-15d: p < 0. 0001, H698D-25d vs WT-25d: p = 0. 9323, H698R-25d vs WT-25d: p < 0. 0001, H698D-35d vs WT-35d: p = 0. 3259, H698R-35d vs WT-35d: p < 0. 0001. (E) Survival time courses of Drosophila adults post infection with M. anisopliae. The median survival days was measured and is listed on the right. Female: WT-LT50 vs H698D-LT50: P = 0. 000, WT-LT50 vs H698R-LT50: P = 0. 006; Male: WT-LT50 vs H698D-LT50: P = 0. 000, WT-LT50 vs H698R-LT50: P = 0. 008. https://doi. org/10. 1371/journal. pgen. 1009861. g002 either shared or unique; all of them were consistent with our bioinformatic analysis (Figs 4C and S5 ). 

Furthermore, we analyzed the distribution of distances between two SSs of the DS events in the RI, A5SS and A3SS groups. First, distance between two SSs in the RI group, or the length of the intron, was highly enriched in a segment of 50-80 nt whose splicing was enhanced by both sf3b1 mutants (ΔPSI > 0. 05), whereas no such enrichment was seen in inhibited RI events (ΔPSI < -0. 05) (Fig 4D left ). Second, in the A3SS group, the distance between two alternative 3 0 SSs was enriched in a segment of 6-30 nt in both sf3b1 mutants (Fig 4D right ). In contrast, the distance between the two alternative 5 0 SSs in the A5SS group was not obviously enriched (Fig 4D middle ). These results suggest that the two sf3b1-H698 mutants not only share similar effects on alternative splicing events but also have different unknown specificities in the recognition of pre-mRNA substrates.
section_name: Differential AS events in the two mutants

section_content: To find affected candidate genes that may cause the climbing defects of the H698R flies (Fig 2D ), we compared DS events between the two mutants for genes that are functionally involved in muscle and neuron development [66, 67]. In comparison to H698D, 125 DS events from 70 muscle-related genes and 70 DS events from 42 neural-related genes are specific to the H698R mutant (S5 Table ). Of these, AS of Gprk2 [68, 69], alien [70] and Mef2 [71] [72] [73], three genes that are important for Drosophila locomotion [67], were significantly changed in H698R, but not in H698D (Figs 5A, S6, S7A and S7C); these were further validated by RT-PCR analyses (Fig 5B ). Interestingly, the identified DS events of the Mef2 gene are tissue specific, showing different AS patterns in the muscle (including the flight, jump and part of leg muscles), head and body from the WT adult (Fig 5C upper ); the AS products with ligation of exons 9+11a in muscles were dramatically decreased in H698R but not in H698D (Fig 5C lower ). These data suggest that the reduced climbing ability could be caused by changed AS events from a number of muscle and neural genes, which would be due to cryptic splicing elements that can be recognized by Sf3b1-H698R but not by Sf3b1-H698D, or alternatively could be due to the altered genes having regular elements that are recognized by Sf3b1-WT and Sf3b1-H698D, but cannot be recognized by Sf3b1-H698R.
section_name: H698R-specifically changes AS events in muscle and neural-related genes

section_content: Alternative splicing of Mef2 is complicated in Drosophila, generating 11 isoforms according to our RNA-seq data and Flybase annotations (S7C and S8 Figs). To address whether it is the changed AS of Mef2 that contributes to the climbing defects, according to annotations of tissue-specific expression in Flybase, we determined that the isoform RB is the most likely downregulated transcript in the sf3b1-H698R mutant flies. Therefore, we constructed transgenic flies of Mef2 promoter-driven GAL4 and UAS-driven Mef2(RB) in the WT and sf3b1-H698 mutant backgrounds, respectively. Additional expression of the Mef2(RB) isoform doubled the climbing ability of the H698R mutant, while only ~15% increase of the climbing ability of the WT and H698D mutant (Fig 5D ), suggesting that the impaired locomotion activity of the H698R mutant is at least partly due to the changed AS of the Mef2 gene. ). We further found that the usagedecreased 5 0 SSs preferentially had weaker splicing signals than the usage-increased 5 0 SSs and usage-not-changed 5 0 SSs (|ΔUSS| < 0. 01) in both the sf3b1 mutants, exhibiting lower strength scores and relatively less conservation of the last two nucleotides (AG) of the 5 0 exon (Figs 6C left and S9); whereas the usage-decreased 3 0 SSs preferentially had stronger splicing signals than the usage-decreased 3 0 SSs and usage-not-changed 3 0 SSs (|ΔUSS| < 0. 01), exhibiting Four types of AS were analyzed. Black dots: overlapped events in both mutants, brown dots: unique in H698D, green dots: unique in H698R. Significantly changed AS: |ΔPSI| > 0. 05, FDR < 0. 05. (C) Validation of AS changes in the two sf3b1 mutant strains by RT-PCR. Overlapped and unique events were selected from the four analyzed AS types. AS isoforms are indicated on the right side of gels. (D) Distribution of distance between two splice sites of the significant changed AS events in sf3b1 mutants. For the RI events, those two are the 5 0 and 3 0 SSs from the intron; for the A5SS events, those are the two alternative 5 0 SSs; for the A3SS, those are the two alternative 3 0 SSs. https://doi. org/10. 1371/journal. pgen. 1009861. g004 We then compared the two data sets of ΔUSS and ΔPSI, and found that the significantly usage-changed 5 0 SSs were highly enriched only in the group of RI events (Fig 6D left ), while the significantly usage-changed 3 0 SSs were highly enriched in both the RI and A3SS groups (Fig 6D right ). These results notably demonstrate that alteration by the sf3b1-H698 mutants on the 5 0 SSs preferentially results in intron retention and on the 3 0 SSs preferentially causes intron retention or alternative 3 0 SS.
section_name: Expression of the Mef2-RB isoform partly rescues the climbing defects of sf3b1-H698R

section_name: Changed recognition

section_content: We previously observed opposite effects on splicing of suboptimal BS substrates between the yeast counterpart mutants Hsh155-H331D and-H331R using the well-established ACT1-CUP1 reporter system [38]. Here, a large number of AS events were similarly changed in both the mutant flies (Fig 4B ). We hypothesized that this might be due to competition between multiple branch sites in the related Drosophila introns. To test this hypothesis, we sequenced nested PCR products of the reverse transcribed lariats from four A3SS events to identify BSs used in the WT and sf3b1 mutants (Figs 7A and S10 and details in Materials and Methods). 

For example, as confirmed by RT-PCRs, an upstream cryptic 3 0 SS in intron 2 of Rilpl was more used, and, to a similar extent, in both sf3b1 mutants in comparison to the WT (Figs 7B and S7E ). Using specific primers, splicing intermediate lariats of the Rilpl-intron 2 from the three fly strains were reversely transcribed and amplified through a two-steps nested PCR. After gel purification and Sanger sequencing of dozens of plasmid clones from each strain, we identified four BSs from this intron according to the new sequences of ligation between the BS and the 5 0 SS where the BS-A is often mutated to T due to reverse transcription of a unique 2 0,5 0 -phosphodiester bond (S10 Fig), only two of them (positions -21 and -23) were used in the WT and their selection was greatly decreased in the two sf3b1 mutants. The H698R mutant preferentially used the most upstream BS at the position -29, whereas the H698D mutant nearly equally selected three other BSs (Figs 7B and S9A ), suggesting a different preference of BS selection by the Sf3b1-WT, -H698D and -H698R proteins. Similarly, multiple selections of BS were also observed in the other two tested shared A3SS events, in which an upstream cryptic 3 0 SS in intron 5 of bip2 and a downstream cryptic 3 0 SS in intron 7 of bol was used more in the sf3b1 mutants than in the WT (Figs 7C and 7D, S7D and S7F). Multiple BSs were identified in these two introns by lariat sequencing. For intron 5 of bip2, in total eight branch sites were used in the three fly strains, in which the WT prefers to use position -25A, the H698D mutant prefers to equally use positions -25A, -30A and -31A, and the H698R mutant prefers to use the most upstream -54U (Figs 7C and S9B ). For the intron 7 of bol, in total six branch sites were used in the three fly strains, in which the WT prefers to use positions -16G and -26A, the H698D mutant prefers to use position -26A, and the H698R mutant prefers to -26A and the most upstream -32C (Figs 7D and S9C ). 

We also tested BS selection in intron 10 of Mef2, in which the changed A3SS only occurs in the H698R mutant. In total, six branch sites were used in the three fly strains, in which the WT prefers to use positions -22G, -25A and -27U, the H698D mutant prefers to use positions -22A, and the H698R mutant prefers to -22A and the most upstream -33A (Figs 7E and S9D ). Taken together, investigation of branch sites selection in those four introns revealed that multiple aberrant branch sites are used in the two sf3b1-H698 mutants, and the far upstream branch sites are preferentially used by the H698R mutant, suggesting a different branch site selection between the two oppositely-charged mutations.
section_name: The sf3b1-H698R mutant activates upstream cryptic BSs

section_content: The high frequency of SF3B1 mutations and their progressive stimulation in many cancers reflect the critical roles of SF3B1 protein in the recognition and selection of intronic splice cDNA from the intronic lariat is transcribed using an intron-specific antisense primer P1 0, and then amplified by two-steps PCRs using primer sets P1 0 + P1 and P2 0 + P2. Separated PCR products are cloned for Sanger sequencing, and the multiple used branch sites (BS, red As) are identified according to positions of junctions between the internal intron and upstream 5 0 SSs. BS selection in the alternatively spliced Rilpl-intron 2 (B), bip2-intron 5 (C), bol-intron 7 (D) and Mef2-intron10 (E) was identified in the sf3b1-WT and mutants. The sites and branch sites and thereby accurate gene expression. In this study, using the Drosophila melanogaster system, we focused on the hotspot disease mutation site His662 and investigated the effects of its mutations at the levels of both splicing and development. 

Structural evidence reveals that SF3B1 has an open-state conformation in the isolated SF3b core [48] and in the 17S U2 snRNP [28], where the super-helical structure of SF3B1 HEATs 1-6 and 9-12 interacts with the N-terminal region of the RNA helicase Prp5. This structural information is consistent with our previous biochemical data in the yeast system [38]. In the cryo-EM structures of B act complex, the SF3B1 HEATs adopt a closed-state conformation of a ring-like structure through which the 3 0 -end of the intron with the branch site is threaded [55, 75, 76]. This conformational change from open to closed state is facilitated by the RNA helicase Prp5. However, the influence on these conformational changes by disease mutations in the SF3B1 HEATs is not yet clear. Differential A3SS events have been found to be the dominant splice changes in other studied SF3B1 mutations, such as K700E, K666 mutations and R625 mutations in human cells lines [40, 47, 59 ] and K700E in mouse models [77] [78] [79]. Here, we found that many AS events in the other four types of alternative splicing are also changed in the Drosophila sf3b1-H698 mutants (Fig 4A ). This could be due to a combination of two reasons: i) the intronic structures including splicing consensus sequences and average intron length in Drosophila are different from those in mammals; ii) the mutated histidine residue may have a different influence on the conformational changes of Sf3b1 during the transition between spliceosomal complexes compared to other HEAT motif mutations. For example, many identified intron retention events in this study are connected with the decreased usage of 5 0 SSs, and those introns are relatively short, a characteristic of fly introns in comparison to mammalian introns. 

Our previous study in yeast found that the Hsh155-H331D mutation decreases yeast Hsh155/SF3B1 interaction with Prp5, whereas the Hsh155-H331R mutation enhances interaction with Prp5, and they have opposite splicing effects on suboptimal BS region substrates, indicating that selection specificities of the branch sites by these two mutants are different. In this study, we found that there are ~1,000 changed AS events in each sf3b1-H698 mutant fly. Sequencing of lariat products from four changed A3SS events, we demonstrate that aberrant branch sites are used in the two sf3b1-H698 mutants, of which the H698R mutant prefers to use far upstream cryptic branch sites, showing a different characteristic from the H698D mutant. The human H662 residue of SF3B1 directly interacts with the intronic 13 th -15 th nucleotides upstream of the branch site adenosine in the Cryo-EM structure of B act complex [55] (S11 Fig). Therefore, we propose that the Drosophila His698 residue mutation to a stronger positively-charged Arg residue results in either a disordered open-state conformation of Sf3b1 that is defective in Sf3b1-Prp5 interaction, or a less stable close-state conformation that alters the pre-mRNA-Sf3b1 interaction (Fig 7F ). Together, the aberrant selection of BS by the two sf3b1 mutations demonstrates that this conserved Histidine residue in Sf3b1 contributes to splicing proofreading at the intronic branch site region. 

Although these two oppositely-charged mutations cause many similar defects during the Drosophila developmental stages, H698D and H698R mutant flies also have different defects, such as innate immune response and movement (Fig 7F ). We find that the H698R mutant specifically alters splicing of many muscle and neuron-related genes, whereas the H698D mutant changed A3SS events were validated by regular RT-PCRs, in which PSI values for DS events (red boxes) in each fly strain are indicated. Selected BSs were identified by lariat RT-PCR and Sanger sequencing. (F) Schematic model of sf3b1-H698D and-H698R mutants that alter selection of the intronic branch sites and result in changed AS, and thereby defective Drosophila respectively. Sf3b1-H698R mutant protein enhances the use of upstream cryptic BSs; this is due to its altered SF3B1 conformation with either changed Prp5-Sf3b1 interaction or changed pre-mRNA-Sf3b1 interaction. Multiple branch sites (red As) and alternative splice sites (red boxes) are indicated. https://doi. org/10. 1371/journal. pgen. 1009861. g007 changes expression of several immune response genes. Expression of the RB isoform of Mef2, which is downregulated in the sf3b1-H698R mutant, partially rescues the climbing defects caused by the H698R mutation (Fig 7F ). 

As mentioned above, previous studies had showed that other sf3b1 mutations resulted in alternative 3 0 SS and non-canonical branch site selection. In this study, we provide data of two H698 oppositely-charged mutations in Drosophila; they have different effects on splicing of a variety of genes, as well as exhibiting different phenotypes. We reveal that far upstream branch sites are used by the H698R mutant, but not by the H698D mutant. These novel findings in Drosophila suggest that mutations at the same residue of SF3B1 in cancers would be mechanistically different in changes of alternative splicing on different substrate genes, and thus would be predicted to have different progression during the development of cancers. 

It has been reported that splicing factor mutations in SRSF2 and U2AF1 result in enhanced R-loops and thereby impaired transcription [80]. Therefore, a portion of those expressionchanged genes in the sf3b1-H698 mutants could be directly caused by altered transcription. In addition, the variety of developmental defects found in this study allow us to expect more SF3B1 mutations to be found in other human diseases.
section_name: Discussion

section_name: Materials and methods

section_content: The wild type (WT) Drosophila melanogaster used in this study is a w1118 isogenic strain (BDSC 5905). Point mutant strains were constructed using the CRISPR/Cas9 system [74]. In brief, the target sequence of each guide RNA (sgRNA) was selected, donor plasmids with point mutations and the adjacent 3 kb sequences as homologous arms were constructed using pMD18-T (Fig 1B ), and the gRNA and donor plasmids were co-injected into embryos of the transgenic line nanos-Cas9 by UniHuaii Technology Company. Specific primers that distinguished point mutations were used for genomic PCRs to screen for the desired alleles, which were further validated by Sanger sequencing of amplicons. The flies obtained were then crossed for at least five generations with the WT strain to eliminate potential off-target events. Homozygous point mutant flies were maintained and cultured on standard cornmeal agar medium. All primers and oligos used are listed in S7 Table. Mef2-GAL4 on the 3rd Chr. was a gift from Prof. Dong Yan at CEMPS, transgenic UAS--Mef2(RB) strain was constructed through incorporating pUAS-T(CDS of Mef2-RB) at attP2 site on the 3rd Chr. Then sf3b1-H698 mutant strains were separately crossed with Mef2-GAL4 and UAS-Mef2(RB), and the strains of sf3b1-H698R/H698R;Mef2-GAL4/UAS-Mef2(RB) and sf3b1-H698D/H698D;Mef2-GAL4/UAS-Mef2(RB) were finally obtained by further crossing.
section_name: Fly strains and culture

section_content: Western blot signals of Sf3b1 and Tubulin were detected using Rabbit anti-Sf3b1 antibody (antigen: VDEDEDDGFPVPQKRT) and anti-tubulin antibody (Sigma), respectively. Fat bodies of third-instar larvae were dissected, followed by incubation with the primary antibody Rabbit anti-Sf3b1 (1:500) and then the secondary antibody goat anti-rabbit Alexa Fluor 594. DAPI (1:2000; Sigma) was used for staining the nuclei. Images were acquired using a Carl Zeiss LSM880 confocal microscope.
section_name: Western blot and immunohistochemistry

section_content: The number of eggs laid per female fly was measured as described [81]. Briefly, ten individual female adults (16-20_hr) from each strain were passed to new vials, and their eggs laid per vial were counted at each time point. Four sets of 300 eggs from each strain were collected and counted for hatching rates under standard condition [82]. Statistical differences were determined according to t-tests. All statistical analyses were performed with GraphPad Prism 7 (San Diego).
section_name: Fecundity and hatching assays

section_content: Homozygous flies were mated and their laid eggs were collected in a 0. 5_hr window and counted as time zero. The 1st instar larvae (270 for each strain) were picked and transferred into new vials with standard food at the 30_hrs post-laying. Pupation and eclosion of flies were counted in regular intervals [82]. Their lifespans were measured as described [81]. Briefly, 200 virgin females and 200 virgin males were maintained in vials at a density of 25 flies per vial on standard food. Flies were transferred to new vials every 2-3 days and the dead flies were counted, and the survival median time was analyzed using GraphPad Prism curves.
section_name: Time of developmental stages

section_content: Climbing ability (negative geotaxis) was measured as described [83]. Ten flies in a vial, three vials of adults at the 5th, 15th, 25th, and 35th days were collected for each strain per assay. Fly climbing was monitored and recorded three times after tapping them to the bottom of the vials, and the height was scored from the photo taken after 6 seconds using RflyDetection software. Multiple climbing flies were processed by Prism. Using a t-test, statistical analyses were presented as mean ± SEM ( � p < 0. 05, �� p < 0. 01, ��� p < 0. 001). The flight and jump muscle of Drosophila were dissected and obtained as described [84].
section_name: Climbing assay

section_content: Fungal infection was carried out with 10 7 spores/ml of Metarhizium anisopliae ARSEF 23 (Ma23). Briefly, after gentle shaking to evenly distribute the spores after bathing, 150 flies per sample were moved into fresh vials with food. Non-infection controls were given the same treatment without fungus. Flies were then kept at 29˚C and transferred to new vials every two days with counting of the surviving flies. Percentages of survivals were presented by the averages with standard errors, and the median survival days were calculated using GraphPad Prism [85].
section_name: Fungal infections

section_content: Total RNAs from the 5d_adults were isolated by TRIzol (Ambion) and treated with RNasefree DNase I (Invitrogen). Construction of cDNA libraries and sequencing were performed using Illumina HiseqXten-PE150 by Novogene. Raw reads from RNA-seq were quality filtered and trimmed, and then mapped to the Drosophila melanogaster genome (dm6) by Hisat [86] and counted by HTseq [87]. 

Analysis of differentially expressed genes was performed by DEseq2, genes with fold changes > 1. 5 and FDR < 0. 05 in both two lines were screened as significant. The Gene Ontology (GO) enrichment is analyzed by online DAVID. Differentially spliced (DS) events were analyzed by rMATS [88]. Significant DS events were screened by conditions |ΔPSI| > 0. 05 and FDR < 0. 05. Differential splice site usage was analyzed by ΔUSS, which is modified from an Unused Index as described [74]. Significant ΔUSS were screened by conditions |ΔUSS| > 0. 01, p value <0. 05. Muscle-related genes were selected from a list as described [67] and the neuralrelated genes were selected from a list as described [66].
section_name: RNA-seq and bioinformatics

section_content: For the regular RT-PCR, reverse transcription was performed using RevertAid Reverse Transcriptase (Thermo), and the cDNA was amplified by Ex-Taq (TaKaRa). qPCR was carried out using SYBR Green Master Mix (Applied Biosystems) in biological triplicates followed by ΔΔCt analysis. 

The muscle samples for RT-PCR were dissected as described [84] with modifications. Briefly, fly adults were anesthetized and placed in the iced PBS buffer with 0. 01% of tween 20, and the head, abdomen and wings were carefully removed. To prepare IFM, two cuts were made to split the thorax open, one was through the ventral cuticle between the two sets of legs and the other was through the dorsal cuticle. The dorsal cut was made off to one side to ensure that the opposite side's set of DLM fibers are not damaged. The exposed IFM was then picked and placed into a tube with 100 μl PBST and maximum 6 individuals. The dorsal TDT was clamped and stretched slightly, and the jump muscle was freed from the cuticle by cutting the middle leg at the coxa and pleura junction. The TDT sample was then picked and placed into a tube with 100 μl PBST and maximum 6 individuals. Since the muscles cannot be remained in PBST for long time, the whole procedure was performed in less than 20 min, and samples were quickly stored in -70˚C freezer. At least 30 flies per strain were dissected to ensure a sufficient number of individuals for further RNA isolation. 

For the lariat RT-PCR, cDNA was synthesized by SuperScript IV Reverse Transcriptase (Invitrogen) using an intron-specific antisense primer P1 0 (positions see Fig 7A ) located close to the 5 0 -end of an intron. The 1 st -step PCR was performed using primers P1 0 and P1 in 25 μl for 30 cycles, and the 2nd-step PCR was carried out with primers P2 0 and P2 in 50 μl after adding 0. 2 μl product from the 1st-step for 35 cycles. One-fourth (12. 5 μl) of the PCR products were fully separated by 4% agarose for visualization of bands; the remaining PCR products were running on 4% agarose for short time, gel purified by an Axygen kit and then subcloned into a T-vector (Takara). To identify branch sites and their usage frequencies, 10-12 subclones from each strain were picked for Sanger sequencing, and their sequences were aligned according to the junctions between 5 0 SSs and BSs. All the primers used are listed in S7 Table.
section_name: RT-PCR, qPCR and lariat RT-PCR

section_name: Acknowledgments

section_content: We thank Prof. Margaret Ho at Shanghai Tech University for help with the climbing assay, and Prof. Chengshu Wang at the Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, for help with fungal infections. We thank other members in the Xu lab for data entries and discussions.
section_name: Acknowledgments

section_content: This work was supported by grants from the National Natural Science Foundation of China to Y. -Z. X ( NSFC 31971225, 31525022 and 91440109 ) and Y. -J. F. ( NSFC 31570821 ), and the Science and Technology Department of Hubei

section_content: All relevant data are within the manuscript and its Supporting Information files. All Next-generation sequencing files are available from the Gene Expression Omnibus (accession number GSE171002).

section_name: Supporting information

section_content: Writing -review & editing: Bei Zhang, Zhan Ding, Ling-Kun Xie, Yu-Jie Fan, Yong-Zhen Xu.
section_name: Supporting information