Hydroxylation of the NOTCH1 intracellular domain regulates Notch signaling dynamics

<jats:title>Abstract</jats:title><jats:p>Notch signaling plays a pivotal role in the development and, when dysregulated, it contributes to tumorigenesis. The amplitude and duration of the Notch response depend on the posttranslational modifications (PTMs) of the activated NOTCH receptor – the NOTCH intracellular domain (NICD). In normoxic conditions, the hydroxylase FIH (factor inhibiting HIF) catalyzes the hydroxylation of two asparagine residues of the NICD. Here, we investigate how Notch-dependent gene transcription is regulated by hypoxia in progenitor T cells. We show that the majority of Notch target genes are downregulated upon hypoxia. Using a hydroxyl-specific NOTCH1 antibody we demonstrate that FIH-mediated NICD1 hydroxylation is reduced upon hypoxia or treatment with the hydroxylase inhibitor dimethyloxalylglycine (DMOG). We find that a hydroxylation-resistant NICD1 mutant is functionally impaired and more ubiquitinated. Interestingly, we also observe that the NICD1-deubiquitinating enzyme USP10 is downregulated upon hypoxia. Moreover, the interaction between the hydroxylation-defective NICD1 mutant and USP10 is significantly reduced compared to the NICD1 wild-type counterpart. Together, our data suggest that FIH hydroxylates NICD1 in normoxic conditions, leading to the recruitment of USP10 and subsequent NICD1 deubiquitination and stabilization. In hypoxia, this regulatory loop is disrupted, causing a dampened Notch response.</jats:p>

section_content: The highly conserved Notch signaling pathway regulates a wide range of biological processes such as immune cell development and function [1] vascular morphogenesis [2, 3] and its deregulation is frequently observed in cancer [4] [5] [6]. Notch signaling is activated by the binding of a Notch ligand to a transmembrane NOTCH receptor. This interaction results in two sequential proteolytic cleavages that lead to the release of the NOTCH intracellular domain (NICD). The NICD subsequently translocates into the nucleus where it interacts with the transcription factor (TF) RBPJ, the coactivator MAML1 (MASTERMIND-LIKE 1), and the acetyltransferase EP300 to drive the expression of Notch target genes [7]. This transcriptional program is regulated by posttranslational modifications (PTMs) of the NICD, including prolyl isomerization [8] [9] [10], influencing the amplitude and duration of the Notch response [11, 12]. 

Cells have developed mechanisms to cope with oxygen (O 2 ) deprivation (hypoxia) by activating the hypoxia-induced factors (HIFs) [13, 14]. Under normoxic conditions, two different 2-oxoglutarate-dependent oxygenases regulate HIF1α: Prolyl hydroxylases (PHDs), that catalyze prolyl (P) hydroxylation of HIF1α, and factor inhibiting HIF (FIH), which catalyzes of asparagine (N) hydroxylation [15, 16]. Proline hydroxylation of HIF1α promotes its proteasomal degradation by the Von-Hippel-Lindau (VHL)-containing E3 ubiquitin ligase complex [13, 14, 17]. FIH-mediated asparagine hydroxylation in the C-terminal activation domain of HIF1α prevents its interaction with the coactivator EP300 [16]. Limited availability of O 2 leads to inhibition of both PHDs and FIH resulting in the stability of HIF1α (and of other members of the same family of transcription factors) and activation of the hypoxiainducible target genes [13, 14, 16, 17]. Interestingly, FIH has a higher O 2 affinity and still functions under intermediate O 2 levels [18]. In addition, FIH seems to be physiologically important in situations with a rapid onset of hypoxia such as ischemia [19]. 

Given the central importance of Notch and hypoxia pathways not only for development but also for homeostasis, it is not surprising that both signaling cascades regulate each other. For instance, hypoxia has been reported to induce the expression of direct Notch target genes of the Hairy Enhancer of the Split family [20] [21] [22] [23] [24] [25] [26] [27] [28], suggesting that hypoxia increases the Notch pathway activity. Moreover, FIH has been shown to hydroxylate NICD within the ankyrin (ANK) repeats [12, 27, [29] [30] [31], providing a potential molecular mechanism for the direct oxygen-dependent regulation of Notch signaling. However, the functional consequences of NICD hydroxylation are not entirely clear. 

Here, we study the consequences of hypoxia on Notch signaling using a mouse progenitor T-cell line, in which the Notch pathway is constitutively active. Unexpectedly, we observe that the majority of Notch target genes are downregulated in conditions of hypoxia correlating with lower NICD1 hydroxylation and protein levels. Using an antibody recognizing site-specific NICD1 hydroxylation, we find that FIH mediates NICD1 hydroxylation. We further show that NICD1 hydroxylation alters its ubiquitination, influencing both degradative and non-degradative ubiquitin chains. Moreover, we observe that the molecular crosstalk between NICD1 hydroxylation and ubiquitination depends on deubiquitinase (DUB) USP10, which modulates Notch responses.
section_name: INTRODUCTION

section_content: Antibody was raised against the hydroxylated asparagine 1945 (N*) of the NICD1 sequence ASADA N* IQDNM and affinity purified with peptides immobilized on sulfolink beads. The serum was first passed over a column with NICD1 N1945-OH peptides; afterward, unspecific antibody was depleted over a column with unmodified NICD1 peptides. The supernatant containing the NICD1 N1945-OH antibody was recovered and dialyzed overnight in PBS. The specificity of the purified antibody was analyzed by dot blot. Peptides were synthesized at Biosynthan and the antibody was produced by BioGenes.
section_name: MATERIALS AND METHODS Generation of the NICD1 N1945-OH antibody

section_name: RESULTS

section_content: We studied the molecular interplay between Notch signaling and hypoxia using the mouse progenitor T-cell line Beko, derived from T-cell receptor knockout mice, in which the Notch pathway is constitutively active [32] [33] [34]. We treated Beko cells for 24 hours with the hydroxylase inhibitor dimethyloxalylglycine (DMOG) or kept the cells for 12 h under hypoxic conditions (1% O 2 ). We observed increased stability of both HIF1α and HIF2α proteins (Fig. S1A, B ). In addition, both regimens elicited similar transcriptional responses: 780 genes were affected in both conditions (Fig. S1C -G and Tables S1, S2 ). Gene set enrichment analysis using KEGG datasets showed that genes associated with the "HIF1 signaling pathway" were significantly enriched in the genes commonly regulated by DMOG and hypoxia (Fig. S1H and Table S3 ). Similarly, "response to hypoxia" and "cellular response to hypoxia" were among the significantly enriched gene ontology (GO) terms of the overlap of upregulated genes (Table S4 ). Gene set enrichment analysis (GSEA) also demonstrated that genes linked to "response to hypoxia" were enriched (Tables S5, S6 ). The upregulation of hypoxia target genes was further validated by qPCR (Fig. S1I, J ). The GSEA analysis also unveiled that genes associated with the "Notch signaling pathway" were significantly changed upon both DMOG and hypoxia treatment (Fig. S2A, B and Tables S5, S6 ). 

To further investigate how Notch signaling is regulated by hypoxia in these cells, we made use of RNA-and ChIP-Seq datasets in Beko cells, in which Notch signaling is inhibited by the γsecretase inhibitor (GSI) DAPT [32]. We focused on genes that were downregulated by GSI and bound by the transcriptional effector RBPJ, assuming that these are direct Notch target genes. Using this approach, we defined 34 genes as bona fide direct Notch targets, including Hes1 and Hey1 encodes for transcriptional repressors, Il2ra (IL2 receptor-alpha, also known as CD25) that encodes for a subunit of the IL2 receptor, which is required for cell-autocrine T-cell homeostasis and Ptcra (preT-cell receptor-alpha) which encodes for the essential subunit of the preT-cell receptor (preTCR) (Fig. 1A, B and Table S7 ). Taking advantage of this Notch signature, we observed that canonical Notch targets Hes1 and Hey1 are upregulated in hypoxic conditions, which is in line with previous reports [12, [20] [21] [22] [23] [24] [25] [26] [27] [28]. However, surprisingly, we observed that the majority of the Notch signature genes are downregulated upon hypoxia or DMOG treatment (Fig. 1A, B and Table S2 ), a finding that was also validated by qPCR (Figs. 1C and S2C, D ). To better understand whether the transcriptional regulation is RBPJ/Notchdependent or HIF1α dependent, we performed ChiP-Seq experiments in Beko cells to determine the localization of transcription factors RBPJ and HIF1α in normoxic and hypoxic conditions (Table S7 ). We observed a significant increase in the binding of HIF1α upon hypoxia (Fig. S3A ) but the genomic binding of RBPJ was hardly influenced (Fig. S3B ). The hypoxia-responsive element (HRE) was promptly identified at the HIF1α binding sites (Fig. S3C ) and we could identify several genes that are deregulated by hypoxia or DMOG and bound by HIF1α (Fig. S3D and Table S8 ). When focusing on the bona fide Notch target genes, we observed that both Hes1 and Hey1 are bound by HIF1α upon hypoxia while the binding of RBPJ is reduced, suggesting that their upregulation is HIF1α dependent (Fig. S3E, F ). 

Together, our data reveal that in the Notch-ON state the majority of Notch target genes are downregulated upon hypoxia. In contrast, Notch targets like Hes1 and Hey1, which are also true HIF1α targets, are upregulated upon hypoxia.
section_name: Hypoxia or DMOG-treatment downregulates expression of Notch target genes

section_content: PTMs of the NICD1 regulate its stability and activity [11, 12]. Given that FIH is an O 2 -sensitive enzyme known to hydroxylate NICD1 [12, 27, [29] [30] [31], we reasoned that FIH might influence NICD1's stability. Indeed, we observed lower NICD1 protein levels after 12 h of hypoxia incubation or 24 h of DMOG treatment (Fig. 2A ) in Beko cells, consistent with the downregulation of the Notch target gene signature (Figs. 1A-C, S2C, D, and Table S2 ). To determine whether these effects are due to changes in NICD1 protein halflife, we performed cycloheximide (CHX) experiments in Beko cells (Figs. 2B and S4H ). We observed that DMOG treatment reduces the stability of NICD1 compared to the DMSO treated controls (Figs. 2B and S4H ). Given that DMOG inhibits both FIH and PHDs [35] [36] [37] [38] [39], we treated Beko cells with roxadustat, a selective inhibitor of PHDs. We observed that roxadustat did not affect NICD1 protein levels (Fig. S4A ), suggesting that the DMOG-and hypoxia-induced effects on Notch signaling are caused by FIH. In support of a functional relationship between FIH and NICD1, we found that these proteins interact with each other in both HEK293 cells (Fig. 2C ) and in Beko cells (Fig. S4B, C ). We further mapped the domains necessary for the FIH/NICD1 interaction and found that it requires the ankyrin-repeats of the NICD1 (Fig. S4D, E ). Finally, using NICD1 and FIH ChIP we demonstrate in Beko cells that both proteins co-occupy enhancers of Notch target genes that are downregulated in hypoxic conditions (Fig. S4F, G ). Altogether, these data suggest that FIH stabilizes the NICD1.
section_name: FIH positively regulates the NICD1 protein stability

section_content: To further understand the regulation of Notch signaling by the FIH-mediated hydroxylation of NICD1, we generated an antibody recognizing hydroxylated NICD1 on N1945 (NICD1 N1945-OH) but not the unmodified protein (Fig. 3A ). To test the specificity of the antibody, we generated HEK293 cells lacking NOTCH1 or just its C-terminal PEST domain important for the ubiquitin-mediated degradation of NICD1 (ΔPEST; Fig. S5A ). Western blotting for NICD1 validated the correct targeting of HEK293 cells showing the truncated protein in the NICD1 ΔPEST cells (Fig. S5A, compare lane 2 to lane 1) but no detectable signal in the NOTCH1 knockout cells (Fig. S5A, compare lanes 3 and 4 to lane 1). Similar results were obtained for the N1945-OH-specific antibody (Fig. 3B, compare lane 2 to lane 1 and lane 3 to lane 1). Importantly, the NICD1 N1945-OH antibody failed to recognize the NICD1 protein when both the NICD1 hydroxylation sites N1945 or N2012 were mutated to alanine residues (NNAA; Fig. S5B lane 3 ) or when only the N1945 was mutated to alanine (N1945A; Fig. S5B lane 4 ). In line with these results, our antibody recognizes a NICD1 mutated only on N2012 (N2012A; Fig. S5B lane 5 ). Altogether, these data validate the specificity of our NICD1 N1945-OH antibody. 

Using this tool, we observed that hypoxia or DMOG but not 24 h of roxadustat treatment lead to a reduction of asparaginylhydroxylated NICD1 in Beko cells (Fig. S5C-E ). To exclude that the reduction of modified NICD1 just reflects a reduction in overall NICD1 protein abundance, we shortened the hypoxia incubation and DMOG treatment. We kept Beko cells for 4 hours under hypoxia or we treated the cells for 4 or 6 h with DMOG. Under these conditions, NICD1 protein levels remained unchanged (Fig. 3C, middle panels) while the levels of N1945 hydroxylated NICD1 were diminished (Fig. 3C, upper panels). These data demonstrate that the reduction in NICD1 hydroxylation precedes the reduction in protein levels and raises the possibility that hypoxia reduces NICD1 stability via its altered hydroxylation. 

Similarly to Beko cells, DMOG treatment or hypoxia in RPMI-8402, a human T-cell acute lymphoblastic leukemia (T-ALL) line, again results in downregulation of Notch target genes (Fig. S6A, B ) associated with decreased NICD1 protein level (S6C, D) and reduced NICD1 hydroxylation (Fig. S6E, F ). 

To assess whether FIH is implicated in this regulation, we generated cells depleted for FIH using CRISPR/Cas9 (Fig. S7A-D ). In FIH-depleted HeLa cells, NICD1 hydroxylation on N1945 was abolished (Fig. 3D, compare lanes 2 and 3 to lane 1). Furthermore, re-expression of wild-type (wt) but not catalytically dead (CD) FIH [40] restored NICD1 hydroxylation (Fig. 3D ). In addition, we also analyzed NICD1 mutants harboring mutations of the hydroxylation acceptor sites N1945 and N2012 (Fig. S7E ). Overexpression of FIH increases NICD1 N1945-OH when the NICD1 wild-type or N2012A mutant but not NICD1 N1945A or NICD1 NNAA mutants are co-overexpressed (Fig. S7E ). Biochemically, the protein stability of the NICD1 NNAA mutant is slightly reduced as shown in CHX assays (Fig. 3E ). Importantly, the mutation of N1945A and N2012A did not impact NICD1's interaction with RBPJ and MAML1 in HEK293 cells (Fig. S8A-D ). Similar to the wild-type NICD1, the mutant also localizes to the nucleus in HeLa cells (Fig. S8E ). In contrast, transactivation of Notch-dependent luciferase reporters was severely compromised (Fig. 3F ). Altogether, these data suggest that the hydroxylation-resistant NICD1 mutant is less stable and less transcriptionally active. 

To further explore the function of hydroxylation-resistant NICD1 in vivo, we tested the NICD1 NNAA mutant in embryonic development using Danio rerio (zebrafish) as well known Notch model system (Fig. 4A, B ). We injected mRNA encoding for N1ΔE wt or NNAA mutant in one-cell-stage zebrafish embryos together with a Notch-dependent GFP reporter plasmid (12x CSLRE-EGFP). In control (vector only) embryos Notch-driven gene expression is on background levels. Wild-type (wt) N1ΔE but not the NNAA mutant is able to drive the expression of the Notch reporter (EGFP) (Fig. 4A ). In line with this, the number of malformed embryos was much higher in presence of the N1ΔE wt compared to the hydroxylation-deficient NICD1 NNAA mutant (Fig. 4B ). Together, these data support the notion that the hydroxylation-defective NICD1 is functionally impaired in vivo in Notch-dependent neurogenesis in D. rerio. 

FIH-mediated NICD1 hydroxylation regulates its stability using a ubiquitination-dependent mechanism Ubiquitination of NICD1 is pivotal to limit Notch responses and is guided by other NICD1 PTMs [11, 32, 33, 41, 42]. Therefore, we investigated whether NICD1 hydroxylation is coupled to ubiquitination. To this end, we used tandem ubiquitin-binding entities (TUBEs) assays [43] to assess ubiquitination patterns of wild-type and NNAA-mutated NICD1. We observed that the NICD1 NNAA mutant shows increased ubiquitination compared to its wild-type counterpart in Phoenix TM cells (Fig. 5A, compare lane 3 to 2). Similarly, we observed an increase in ubiquitination of the endogenous NICD1 upon DMOG treatment of Beko cells (Fig. 5B ). Interestingly, the NICD1 ubiquitination was also increased in the NICD1 NNAA mutants that lack the destabilizing OPA-PEST domain in Phoenix TM cells (Fig. 5C, compare lane 7 with 9 and lane 8 with 10), suggesting that there is additional ubiquitination beside of degradative K48-linked ubiquitination that is known to occur within the PEST domain [44, 45]. While K48-linked ubiquitination plays key roles in proteasomal degradation, ubiquitination through K11 or K63 has important signaling functions [46]. To further unravel the link between NICD1 hydroxylation and ubiquitination, we used ubiquitin (Ub) mutants in which specific lysines were mutated to arginines (K11R, K48R or K63R) or mutants in which all the lysines with the exception of a single one were mutated to arginines (K11 only, K48 only and K63 only). In Phoenix TM cells, we observed that the increased ubiquitination of the NICD1 NNAA mutant in comparison to the NICD1 wt was abolished with the K63R Ub mutant (Fig. S9A, compare lane 13 with 12) and slightly reduced with the K11R Ub mutant (Fig. S9A, compare lane 15 with 12) but hardly influenced in presence of the K48R mutant (Fig. S9A, compare lane 14 with 12). In addition, in presence of Ub K63 only, the ubiquitination of the NICD1 NNAA was retained even if slightly reduced (Fig. S9B, compare lane 13 with 12). In presence of the Ub K48 only, the ubiquitination of the NICD1 NNAA mutant was almost completely abolished (Fig. S9B, compare lane 14 with 12) even if still higher than the one of the NICD1 wt in presence of the Ub K48 only (Fig. S9B, compare lane 14 with 10). In presence of the Ub K11 only, the ubiquitination of the NICD1 NNAA mutant was strongly reduced (Fig. S9B, compare lane 15 with 12) but higher than the one of the NICD1 wt in presence of the Ub K11 only (Fig. S9B, compare lane 15 with 11). Altogether, these data suggest that NICD1 hydroxylation prevents its ubiquitination and that the increased ubiquitination of the NICD1 protein is mainly occurring through Ub K63 and to a minor but still significant extent to Ub K11 while there is a minimal increase through Ub K48.
section_name: Characterization of the FIH-mediated asparaginyl hydroxylation of the NICD1

section_content: To further explore the link between NICD1 hydroxylation and ubiquitination, we focused on the E3 ubiquitin ligase FBXW7, which targets NICD1 for proteasomal degradation [42, 44]. We hypothesized that NICD1 hydroxylation affects the NICD1/FBXW7 interaction and hence impairs NICD1 ubiquitination. We performed co-immunoprecipitation experiments in Phoenix TM cells co-expressing FBXW7 as well as Flag-tagged NICD1 wild-type (wt) or NNAA mutant (Fig. S10A ). We observed no major changes in the interaction between FBXW7 and NICD1 NNAA when compared to the NICD1 wild-type (Fig. S10A, compare lane 5 with 6), suggesting that the increased NICD1 ubiquitination is not due to enhanced FBXW7 recruitment. Protein ubiquitination is reversed by deubiquitinases (DUBs), which cleave ubiquitin (chains) from their substrates. USP10 has been shown to regulate NICD1 ubiquitination [47]. Of note, we found in our RNA-Seq analysis that the expression of the USP10-encoding gene is downregulated in hypoxic or DMOG-treated Beko cells (Table S2 ). Similar results were obtained at the protein level in Beko cells (Fig. 6A ). However, no expression changes were noted for FBXW7 in Beko cells (Fig. S10B, C ). Furthermore, we observed that USP10 interacted less efficiently with NICD1 NNAA than with the NICD1 wild-type in Phoenix TM cells (Fig. 6B, compare lanes 5 to 6), suggesting that the hypoxia-induced changes in NICD1 ubiquitination and stability result from impaired deubiquitination. These data suggest a model, whereby FIH-mediated hydroxylation determines the ability of NICD1 to interact with USP10, a DUB, whose expression is downregulated by hypoxia. In addition, we observed that Usp10 knockdown in Beko cells leads to reduced NICD1 protein levels (Fig. 6C left) and to reduced expression of Ptcra, Uaca, and Il2ra Notch target genes (Fig. 6C middle). In line with that, GSEA analysis upon Usp10 knockdown in Beko cells followed by RNA-Seq (Fig. S11A and Tables S1, S2 ) demonstrated that genes linked to the "Notch signaling pathway" were enriched (Fig. S11B and Table S9 ). In addition, a general downregulation of the Notch response was observed when looking at bona fide Notch target genes in our RNA-Seq analysis (Fig. 6C right, S11C, and Tables S1, S2 ). 

Together, our data strongly suggest that USP10 stabilizes NICD1 in Beko cells through a hydroxylation-sensitive mechanism.
section_name: The interplay between NICD1 hydroxylation and ubiquitination involves the deubiquitinase USP10

section_content: Regarding the interplay between Notch signaling hypoxia, we have uncovered that downregulation of Notch target genes is due to reduced protein stability. In normoxic conditions, FIH hydroxylates the NICD1 affecting ubiquitination regulated by E3 ubiquitin ligase FBXW7 and deubiquitinase USP10 (see also model in Fig. S12 ). Upon hypoxia, NICD1 hydroxylation ceases and the USP10 protein level is reduced, leading to perturbed NICD1 ubiquitination and subsequent downregulation of Notch target genes. 

Seemingly contradictory to our data, several other studies have suggested hypoxia as an inducer of the Notch signaling pathway [20] [21] [22] [23] [24] [25] [26] [27] [28]. However, these studies focused only on the Hairy Enhancer of Split family of genes as a readout of Notch activation, which have later been shown to be also regulated by HIF1α and other signaling pathways [12, 26, 28]. For example, it was shown that mutations of the RBPJ binding motif do not prevent the hypoxia-mediated induction of Hes1 [28], supporting the notion that this upregulation is Notch-independent. In line with this, we observe the binding of HIF1α upon hypoxia at Hes1 and Hey1. Thus, taking a genome-wide approach we disentangle RBPJ/NICD from HIF1α-regulated target genes. 

Mechanistically, NICD1 protein stability is affected by hypoxia and postulates a link between NICD1 hydroxylation and ubiquitination. We propose that not only the hypoxia-regulated enzyme FIH but also the deubiquitinase USP10 play a key role in this process resulting in a shifted balance to enhanced NICD1 ubiquitination, in particular, regulatory ubiquitination (see also model in Fig. S12 ). According to our model, NICD1 hydroxylation provides a docking site for USP10, which in turn deubiquitinates NICD1. 

It remains to be determined which E3 ubiquitin ligase counteracts USP10. Apart from FBXW7, known to control degradative ubiquitination, possible candidates are ITCH, MDM2, and/or RNF8, that have been previously linked to NOTCH ubiquitination [48] [49] [50] [51]. 

Our data reveal that hypoxia affects not only HIF1α but also the NICD1 coactivator. In our view, this has both physiological and potentially also pathophysiological implications. Physiologically, NOTCH1 is essential in early T-cell development in the thymus [52, 53], which is known to be highly proliferative with low O 2 tension [54, 55]. In our view, this hypoxic condition could contribute to the modulation of Notch responses, which is pivotal for subsequent T-cell maturation. Our results showing reduced expression of Ptcra and Il2ra (encoding for CD25) support this hypothesis. Regarding the pathophysiological context, our findings are particularly relevant to Notch-driven leukemias, T-cell acute lymphoblastic leukemia (T-ALL), chronic lymphocytic leukemia (CLL), for which NOTCH1 mutations been previously described [56, 57]. They could also play a role in the setting of solid cancer such as breast cancer and squamous cell carcinoma, where active Notch signaling has been described [4, 9, 58]. Future genome-wide analyses should not only focus on HIF1α but also on Notch-dependent gene regulation. 

Together, our data suggest that NICD1 hydroxylation determines not only the strength but also the duration of Notch responses through an FIH-and USP10-dependent mechanism.
section_name: DISCUSSIONS

section_name: ACKNOWLEDGEMENTS

section_content: We are grateful to T. Schmidt-Wöll for excellent technical assistance. The authors wish to acknowledge Centro de Análisis Genómico (CNAG-CRG), Spain, for sequencing the RNA-Seq samples. The authors wish to thank Tobias Zimmermann ( University of Giessen, Germany ) for giving access to the PETRA package and Prof. Dr. L. Schmitz ( University of Giessen, Germany ) for providing reagents.
section_name: ACKNOWLEDGEMENTS

section_name: FUNDING

section_content: This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation )- TRR81-A12 and BO 1639/9-1, the Behring-Röntgen foundation and Excellence Cluster for Cardio-Pulmonary System (ECCPS) in Giessen to TB. Funding for the open access charge was provided by the DFG collaborative research TRR81. This work was further supported by the DFG ( JU2859/2-1 ) to SJ, ( SFB1074/A3, GRK2254/C4, and OS 287/4-1 ), the Deutsche Krebshilfe (# 70114289 ) to FO, (# 70114291 ) to DM, and the German Federal Ministry of Education and Research (BMBF) (e:Med-SYMBOL-HF grant # 01ZX1407A ) to SJ. LP is a participating member of the International Graduate School in Molecular Medicine at Ulm University (IGradU ), which is supported by the DFG (grant number GSC 270 ). BDG is supported by a research grant from the University Medical Center Giessen and Marburg (UKGM) and by a Prize from the Justus Liebig University Giessen.
section_name: FUNDING

section_name: DATA AVAILABILITY

section_content: ChIP-Seq and RNA-Seq developed in the current study have been deposited at GEO under the accession number GSE194003. Detailed Materials and Methods are available in the supplement file.
section_name: DATA AVAILABILITY

section_name: AUTHOR CONTRIBUTIONS

section_content: FF, BDG, LP, and FO performed experiments and analyzed data. BDG, TF, and MB performed the bioinformatic analysis. TS and MP provided reagents. DM and SK generated the HEK293 cells depleted of the NOTCH1 gene. BMG and SJ performed the in vivo experiments. FF, BDG, and TB designed experiments and wrote the manuscript with contributions from other authors.
section_name: AUTHOR CONTRIBUTIONS

section_content: The authors declare no competing interests.
section_name: COMPETING INTERESTS

section_content: The online version contains supplementary material available at https://doi. org/10. 1038/s41419-022-05052-9. 

Correspondence and requests for materials should be addressed to Tilman Borggrefe. 

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section_name: ADDITIONAL INFORMATION Supplementary information