Ibrutinib and venetoclax target distinct subpopulations of CLL cells: implication for residual disease eradication

<jats:title>Abstract</jats:title><jats:p>Ibrutinib inhibits Bruton tyrosine kinase while venetoclax is a specific inhibitor of the anti-apoptotic protein BCL2. Both drugs are highly effective as monotherapy against chronic lymphocytic leukemia (CLL), and clinical trials using the combination therapy have produced remarkable results in terms of rate of complete remission and frequency of undetectable minimal residual disease. However, the laboratory rationale behind the success of the drug combination is still lacking. A better understanding of how these two drugs synergize would eventually help develop other rational combination strategies. Using an ex vivo model that promotes CLL proliferation, we show that modeled ibrutinib proliferative responses, but not viability responses, correlate well with patients’ actual clinical responses. Importantly, we demonstrate for the first time that ibrutinib and venetoclax act on distinct CLL subpopulations that have different proliferative capacities. While the dividing subpopulation of CLL responds to ibrutinib, the resting subpopulation preferentially responds to venetoclax. The combination of these targeted therapies effectively reduced both the resting and dividing subpopulations in most cases. Our laboratory findings help explain several clinical observations and contribute to the understanding of tumor dynamics. Additionally, our proliferation model may be used to identify novel drug combinations with the potential of eradicating residual disease.</jats:p>

section_content: Chronic lymphocytic leukemia (CLL) is the most common leukemia/lymphoma among older adults. It is a chronic lymphoid malignancy with an accumulation of monoclonal mature B-cells in peripheral blood (PB), bone marrow (BM), and secondary lymphoid tissues, such as spleen and lymph nodes (LN) 1, 2. 

Molecularly targeted therapies have produced remarkable therapeutic benefits in CLL. Ibrutinib is a small molecule inhibitor of Bruton tyrosine kinase (BTK), which plays a key role in the B-cell receptor (BCR) signaling. Venetoclax, on the other hand, is a highly selective inhibitor of the anti-apoptotic protein BCL2. Both drugs are highly effective as monotherapy against CLL [3] [4] [5] [6], and clinical trials of the combination therapy are ongoing. An interesting clinical observation is that sensitivity of tumor cells to these two drugs depends on anatomic compartments. In general, many patients receiving ibrutinib experience rapid reduction in lymphadenopathy while patients exposed to venetoclax frequently have tumor lysis. These observations suggest lymph node-resident CLL cells are more sensitive to the action of ibrutinib, while circulating CLL cells in the PB are more sensitive to the action of venetoclax. When these two drugs are used in combination to treat relapsed/refractory 7 or previously untreated CLL patients 8, the rates of complete response were significantly higher than those reported for either single drug alone. Importantly, undetectable BM minimal residual disease (uMRD), which is rarely observed with ibrutinib alone, has been achieved in patients, and the rate of BM uMRD continues to increase over time. 

However, the reasons behind these observed differential compartmental responses and how it is related to the tumor cellular behavior is largely unknown. A better understanding of how these two drugs synergize would eventually help develop other rational combination strategies aimed at eliminating CLL cells from all anatomic quarters. 

Concerning the cellular behaviors of CLL, it has been historically thought that CLL is a disease with defects in apoptosis since tumor cells isolated from the peripheral circulation are dormant and non-proliferating. In the last several years, it has been increasingly recognized that cell proliferation also plays an important role in the CLL pathogenesis 9. Using heavy water tracing of the leukemia cells in patients, it was shown that up to 1% of circulating CLL cells (~1 × 10 9 cells) are newly generated each day from the BM and LN 10. At these tissue sites, tumor cells, with the support of the tumor microenvironment, are actively proliferating, particularly in histological tissue areas identified as proliferation centers 11, 12. Proliferation centers are the major histological sites for CLL pathogenesis, progression, and transformation 13. Larger centers with high proliferation index predict aggressive clinical behavior and poorer patient survival 14. 

Due to easy access, primary tumor cells isolated from the PB of CLL patients are most widely utilized for the purpose of drug testing. The ex vivo approach is costeffective and is achievable in a short time frame, but it does not reproduce tumor in vivo behaviors. Although CLL has become known as a disease with increased proliferation, isolated PB CLL cells are non-proliferative and undergo spontaneous apoptosis in 2-7 days ex vivo 15. Drug testing normally evaluates if a particular agent induces additional apoptosis of isolated CLL cells, but frequently such agents turn out to be ineffective in humans. On the contrary, as a successful drug, ibrutinib generated only an average of 10% additional cell killing in a cohort of patients when tested on PB CLL cells 16. These observations suggest that apoptosis testing with PB is highly unreliable and clinically irrelevant. 

With the increasing understanding of the tumor microenvironment, attempts have been made to recreate the tissue tumor niche in vitro. In the LN microenvironment, CLL tumor cells interact with T cells, dendritic cells, and various other types of stromal components [17] [18] [19]. It has been shown that the tumor microenvironment protects CLL cells from spontaneous and drug-induced apoptosis [20] [21] [22]. Protection by the microenvironment may also be responsible for the persistence of minimal residual disease (MRD), and shorter progression-free and overall survival in treated patients 23. 

In this study, using an ex vivo model that is capable of promoting robust CLL proliferation, we investigated how CLL cells respond differently to ibrutinib, venetoclax, and the combination. Our results revealed that ibrutinib and venetoclax act on distinct subpopulations of CLL cells, and help explain several clinical observations related to the use of these highly effective drugs.
section_name: Introduction

section_name: Materials/subjects and methods

section_content: For CLL cell isolation and ex vivo co-culture, Rosette-Sep™ Human B Cell Enrichment Cocktail was purchased from Stemcell Technologies (Vancouver, Canada), Dynabeads™CD3 from Thermo Fisher Scientific (Waltham, MA), CpG (ODN2006) from InvivoGen (San Diego, CA), Recombinant human Interleukin-15 (IL-15) from Gemini Bio-Products(West Sacramento, CA), and Cell-Trace™ Violet Cell Proliferation Kit from Thermo Fisher Scientific (Waltham, MA). For flow cytometry analysis, FITC anti-human CD5 (Cat# 300606), PE anti-human CD19 (Cat# 302254), APC anti-human CD3 (Cat# 300458), APC anti-human CXCR4 (Cat# 306510) and Alexa Fluor 647 anti-human Ki67 (Cat# 652408) antibodies were purchased from Biolegend (San Diego, CA). Propidium iodide from Sigma (St. Louis, MO). For drug treatment, ibrutinib and venetoclax were purchased from Selleckcham, (Houston, TX).
section_name: Reagents and antibodies

section_content: CLL patient PB samples were collected after informed consent according to the Declaration of Helsinki. The study was approved by the Institutional Review Board of the University of Chicago. Clinical characteristics of the patients were shown in Table 1. CLL cells were isolated by negative selection using Rosettesep TM human B cell enrichment cocktail following the manufacturers' instructions as previously described 15. After isolation, CLL cell purity was assessed using flow cytometry and was >95% CD19+/CD5+ in all cases. Cell viability was assessed with MUSE Count & Viability Kit and was ≥90% in all cases.
section_name: Clinical samples and CLL cell isolation

section_content: The BMF cell line was generated from a long-term culture of the BM cells from a CLL patient without further manipulation. BMF is maintained in RPMI-1640 with 10% fetal bovine serum under standard conditions (5% CO 2, 37 °C). Further characterization indicates that it is a normal human fibroblast exhibiting monolayer growth and contact inhibition. The cell line is positive for BM mesenchymal cell marker CD44, CD29, and CD71 24. Conventional cytogenetic analysis revealed a karyotype of 45, X, -Y, which is consistent with the profile of the patient (73 year-old male with the loss of Y chromosome, a phenomenon frequently noted in elder males).
section_name: Generation and maintenance of BM fibroblast (BMF) cell line

section_content: BMF were trypsinized and seeded onto 12-well plates (2 × 10 5 cells/2 mL/well) to reach 70-90% confluence on the next day. CLL cells were then added to the BMF monolayer in RPMI-1640 media containing 20% FBS, 50 U/mL penicillin, 50 mg/mL streptomycin and 2 mmol/ L of L-glutamine (Mediatech. VA). Plating ratio of CLL to stromal cells fall in the range of 2:1-20:1 depending on the cell number availability in the CLL samples. After 72 h of co-culture, CLL cells were collected by gently pipetting, washed and resuspended in 1 mL media. The residual T cells were removed by Dynabeads®CD3 kit according to manufacturer's protocol. In brief, CD3 Dynadeads were added at 50 µL per mL cell suspension, and rotated at 4 °C for 30 min. The bead-bound CD3+ T cells were separated from cell suspension by EasySep™ Magnet. 

After CD3 depletion, CLL cells were labeled with violet-CFSE using CellTrace™Violet Cell Proliferation Kit according to manufacturer's protocol. Briefly, 10 µL of 5 mM CFSE in DMSO was added into 10 mL pre-warmed PBS for each labeling. CLL cells were washed twice with PBS and re-suspended in 10 mL CFSE-PBS solution. After a 20-min of incubation at 37 °C, cells were spun down, washed once and kept in RPMI-1640 media with 20% FBS for the next step.
section_name: Pre-culture of CLL cells with BMF, T-cell depletion and CFSE labeling

section_content: Cell stimulation and drug testing were conducted in 24well plates with BMF monolayer. For activation of CFSElabeled CLL cells, CpG (2 µg/mL) and IL-15 (10 ng/mL) were added into each well except the unstimulated control. For drug treatment, ibrutinib or venetoclax, at clinically achievable concentrations, was added to the coculture 24 h after CpG/IL-15 addition. For the control well, an equal volume of DMSO was added. At day 7 of the stimulation and drug treatment, 400 µL of cultured CLL cells were collected from each well for flow cytometric analysis.
section_name: Cell stimulation and drug treatment

section_content: Cell survival and proliferation were determined by flow cytometry using LSR2 flow cytometer (BD Biosciences). Briefly, CLL cells collected at day 7 of drug treatment were stained with FITC-anti-CD5, PE-anti-CD19, APCanti-CD3 antibodies, and PI solution was added after the antibody staining and before the flow cytometry analysis. Flow acquisition was conducted for the fixed time duration (60 s). Data were analyzed using FlowJo software (Version 10; TreeStar). Live CLL cell number was calculated by counting CD19 + /CD5 + and PI -events in comparison to the DMSO control. For CLL proliferation, the percentage of cells distributed in the dividing phases of the CFSE profiles is automatically calculated by the FlowJo software. For Ki67 staining, CLL cells were first stained with FITC-anti-CD19 antibody, fixed and the permeabilized prior to Alexa Fluor 647 anti-human Ki67 antibody staining.
section_name: Cell survival and proliferation analyses

section_content: Statistical analysis of the data was performed using Graphpad Prism 8 software (GraphPad, La Jolla, CA, USA). Two group comparisons were conducted using nonparametric paired t-test selected by the Prism 8 software since data points are not normally distributed. One-way ANOVA analysis was applied for comparison among multiple groups and F-ratios are shown when they are indicated. P-values of 0. 05 are considered statistically significant.
section_name: Statistical analysis

section_name: Results

section_content: CLL cells isolated from the PB normally survive 2-7 days in vitro without proliferation 15. Previously, we have constructed a NKTert cell line-CLL co-culture model that supports tumor cell proliferation as evidenced by the appearance of a BrdU + cell population [25] [26] [27] [28]. However, BrdU incorporation merely measures DNA synthesis, one step of several in the cell division process. In order to visualize distinct generations of cell division, the model was further improved with a fibroblast cell line (BMF) established from the BM of a CLL patient and with added stimulants, CpG oligonucleotides and IL-15 22, 29, 30. In this model, CLL cells, when co-cultured with BMF alone, were maintained alive but showed no signs of cell division as assayed by CFSE staining (Fig. 1A, top panels). In contrast, when CLL cells were co-cultured with BMF plus CpG/IL-15 stimulation, several generations of daughter cells appeared. Cell division started on day 3 and cells continued to proliferate until day 28 (Fig. 1A, compare top vs. bottom panels). The culture, in some cases, survived and proliferated for 10 weeks before cell death ensued. 

Under light microscopy, stimulated cells appeared larger and formed clusters (Fig. 1B, compare a with c at 10×, b with d at 20×). In addition, CpG/IL15-stimulated cells, assayed by flow cytometry, were indeed larger compared to cells co-cultured with BMF alone. Figure 1C-a shows change in cell size of a typical case and Fig. 1C-b shows the aggregate data of 17 cases with or without the stimulation. The stimulated cells also expressed lower levels of surface CXCR4 as shown in Fig. 1D-a, b. Downregulations of CXCR4 has been associated with CLL cells that are more proliferative 22, 31. Taken together, data from CFSE labeling, light microscopy, cell size, and CXCR4 expression demonstrate that CLL cells are actively proliferating and dividing in our co-culture model. Although the actual lymph node architecture is far more complex, with its ability to promote long-term cell proliferation, the model recapitulates at least one of the most salient features observed histologically in the "proliferation centers" of the LN. We thus refer this model as the CLL proliferation model.
section_name: CLL cells actively divide for a long-term in a co-culture model

section_content: Previously, using a less advanced co-culture model, we demonstrated that ibrutinib inhibits the appearance of BrdU-positive CLL cells [25] [26] [27] 32 conditions including type of stromal cells and time points (data shown in a separate manuscript), we are able to investigate how ibrutinib affects cell division, which has not been studied before in the absence of a model tool. We tested the effects of the drug on cell division as well as on cell viability in samples taken from 22 ibrutinib-naïve patients and eight ibrutinib-resistant patients (Table 1 ). Only a fixed concentration of 400 nM was used to preserve the clinical samples for multiple assays. The concentration was selected based on the C max (420 nM) derived from the published human pharmacokinetic studies [33] [34] [35]. Figure 2A shows four cases of ibrutinib-naïve patients. In case UCLL053, after 7-day of culture, cell viability was essentially not changed from the vehicle control (Fig. 2A left panel. 100% vs. 106%). In contrast, the CFSE profile shows that generations of dividing cells were significantly scaled down from 48. 2% to 1. 63% with ibrutinib treatment (compare blue vs red trace). Study of additional cases revealed that ibrutinib treatment caused variable changes in cell viability-minimal changes in UCLL053, decrease in UCLL047, and even increases in UCLL046 and FCLL012 (see "Discussion" section). In comparison to viability, ibrutinib caused a significant and consistent suppression of cell division in all four cases (also see aggregate data later in Fig. 2C ). 

We also studied the behaviors of cells harvested from ibrutinib-resistant patients. Six of the eight ibrutinibresistant patients carried the BTK C481 mutation at the time of disease relapse 25, 36 (Table 1 ). As shown in Fig. 2B, in UCLL052, cell viability was reduced from 100 to 77% comparing DMSO vs. ibrutinib-treated cells. CFSE profile, however, shows that ibrutinib decreased cell proliferation from 87 to 68%. Notably, cell division is much less inhibited in this ibrutinib-resistant case comparing to ibrutinib-naïve patients. Similar observations were also made in other cases where suppression of cell proliferation occurred to a much lesser degree compared to ibrutinib-sensitive cases. 

The aggregate data for all 22 ibrutinib-naïve and 8 ibrutinib-resistant patients demonstrate, despite some patient-to-patient variation, lack of a significant inhibition of ibrutinib on cell viability, in both ibrutinib-naïve and ibrutinib-resistant patient cohorts (Fig. 2C-a, b, p = 0. 44 and 0. 22, respectively). This finding is consistent with previous data by us and other groups showing ibrutinib does not induce direct cell death 25, 26, 32, 37. In comparison, ibrutinib markedly reduced proliferation in cells taken from ibrutinib-naïve patients (Fig. 2C-c, p < 0. 0001). In ibrutinib-resistant cases, such inhibition occurred at a lower degree and at a lower frequency, so that when the resistant patients were analyzed as a cohort, the inhibition was not as significant (Fig. 2C-d, p = 0. 046). Overall, these data demonstrate cell division responses to ibrutinib are highly correlated with patients' actual clinical responses to ibrutinib while the conventional cell viability responses are not. Thus, the validity of ibrutinib response readouts by the CLL proliferation model are well supported by patients' clinical response data.
section_name: Only the dividing subpopulation of CLL responds to ibrutinib, which is largely consistent with patients' actual clinical response

section_content: With the CLL proliferation model, we also evaluated how the BCL2 inhibitor venetoclax works. As shown in Fig. 3A, in an ibrutinib-naïve case, UCLL046, venetoclax, as expected, markedly decreased cell viability from 100 to 30%, at a clinically achievable concentration of 200 nM (Fig. 3A, top left panel, blue vs. green columns). The concentration was selected according to venetoclax human PK studies 4, 38. CFSE profiles of the residual live cells, however, revealed an interesting pattern of distribution (Fig. 3A, middle and right panels), with 97% of dividing cells and 3% of resting cells (green trace). This is in contrast to the respective 66 and 34% in the DMSO control, and 8. 7 and 91% in ibrutinibtreated cells. Similar observations were made in another ibrutinib-naive case, UCLL047. Regarding ibrutinibresistant cases, in UCLL052, venetoclax reduced cell viability from 100 to 37%, and of the remaining live cells, >99% were cells in proliferation and <1% were resting (Fig. 3B, green trace). A similar pattern was also noted in UCLL034, another ibrutinib-resistant case. These data suggest that venetoclax induces cell death, but it preferentially kills the resting CLL subpopulation, which is opposite to the action of ibrutinib.
section_name: The resting subpopulation of CLL, instead of the dividing one, preferentially responds to venetoclax

section_content: With these initial findings that ibrutinib and venetoclax may preferentially act on distinct subpopulations of CLL, we tested the effects of either single drug alone or ibrutinib/venetoclax combination side-by-side in all collected cases. Regarding cell viability, Fig. 4 shows several ibrutinib-naive cases while Fig. 5 demonstrates several ibrutinib-resistant cases. Again, ibrutinib showed a highly variable effect on the number of live cells (Figs. 4 and 5, red columns). Venetoclax, on the other hand, reduced the total number of live cells in every single case evaluated (Figs. 4 and 5, green columns, 1-68% relative to DMSO), irrespective of their sensitivity to ibrutinib, which is consistent with its mechanism of action as a BCL2 inhibitor. 

As to cell proliferation, with additional cases evaluated, it became evident that ibrutinib reduced the number of dividing cells in sensitive cases (Fig. 4, blue vs. red traces), 48. 2-1. 63% in UCLL053, 66. 4-11. 0% in CCLL015, 86. 5-16. 9% in FCLL009 and 62. 5-4. 1% in FCLL003. In comparison, this inhibition was not as effective in the ibrutinib-resistant cases (Fig. 5, blue vs. red traces), 43. 1-32. 3% in UCLL041, 87. 3-68. 0% in UCLL052, 59. 8-59. 7% in NCLL018 and 80. 5-64. 8% in UCLL034. With regards to venetoclax, notably, the resting CLL cells, as opposed to dividing cells, are the subpopulation mainly targeted by venetoclax. The proportion of the resting cells ranged from 0. 6% in UCLL052 to 18. 7% in FCLL003 while the dividing cells made up 81. 3-99. 4% of the total cell populations (Figs. The combination of the two drugs, as expected, worked most effectively, significantly reducing total number of live cells (1-26%) in the vast majority of cases irrespective of ibrutinib-sensitivity (Figs. 4 and 5, pink columns). Analyses of CFSE profiles became less meaningful in many of these cases due to the small number of residual live cells left after the combined treatment. 

Figure 6A shows the aggregate cell viability data on 22 ibrutinib-naïve and eight ibrutinib-resistant cases. It is apparent that ibrutinib did not exert any significant effects on cell viability (p value, not significant), Venetoclax, on the other hand, markedly decreased the number of viable cells in both ibrutinib-naïve (left, p < 0. 001) and ibrutinibresistant cases (right, p < 0. 01). The combination of ibrutinib and venetoclax, thus far, produced the most pronounced cell killing (p < 0. 001 in both cohorts). 

Aggregate cell proliferation data are shown on 15 ibrutinib naïve and six ibrutinib-resistant cases (Fig. 6B ). 

Such analyses for cases with few live cells (<100 events) after venetoclax treatment were excluded since they may not be statistically meaningful. It is clearly evident that ibrutinib significantly reduced the percentage of proliferating CLL cells in sensitive but not resistant cases (Fig. 6B, left vs. right panel p < 0. 001 vs. ns). Venetoclax, on the other hand, increased the proportion of the proliferating cells (i. e., decreased the proportion of the resting cells) among the residual live cells in both ibrutinib-naïve and ibrutinib-relapsed cases (Fig. 6B, left and right, p = 0. 001 and p < 0. 05 respectively). 

To further validate these findings, we performed additional analyses of Ki67 expression using flow cytometry. Ki67 is one of the most established and widely used biomarkers for cell proliferation. Compared to CFSE analysis, Ki67 is an earlier marker of cell proliferation and flow cytometric analysis of its expression displays a continuous cell population rather than distinct populations of resting vs. dividing cells. Despite this, the results show that percentages of Ki67 + cells were increased by CpG/IL-15 stimulation, and reduced by ibrutinib but not by venetoclax treatment (Fig. S1 ). These data corroborate our conclusion derived from the CFSE data. 

Taken together, the side-by-side comparisons by CFSE staining, for the first time, demonstrate that ibrutinib and venetoclax target distinct subpopulations of CLL cells with different proliferative capacities. The data imply that the inability of each of the drugs to act on the entire CLL population may account for the persistence of residual disease. These data collectively provide a strong laboratory rationale that combining these two drugs may have the potential for a cure, consistent with the current clinical observations (see "Discussion" section below).
section_name: Combination of ibrutinib and venetoclax effectively eliminates both resting and dividing CLL subpopulations

section_content: In summary, using an ex vivo model that promotes CLL cell proliferation, we demonstrated that ibrutinib primarily targets CLL proliferation/division as opposed to inducing cell death, while venetoclax primarily induces cell death, but preferentially the death of resting cells. Treatment of tumor cells with either drug left behind a subpopulation of CLL cells which is indifferent to the monotherapy while the combination essentially eliminates the vast majority of tumor cells. Regarding cell viability, in some cases we observed an unexpected increase after ibrutinib treatment relative to the DMSO control (Fig. 2A ). This might be explained by the known inhibitory effect of ibrutinib on cell adhesion 39, 40. Upon treatment with ibrutinib, the live, proliferative CLL cells may become detached from the BMF and released into the media. 

The novelty of the work lies on the new model that induces CLL proliferation (Model construction detail will be described separately). Although cell proliferation is not at all a new concept for tumors in general, for CLL, an indolent disease, this property was rarely measured before due to the lack of suitable models. CLL is not merely an indolent disease, tumor cells indeed form "proliferation center" in the lymph nodes. Equipped with the new proliferation assay, we were able to separate cells into dividing and resting subpopulations and see how particular drugs act on subpopulations that has never been demonstrated previously. 

Previous studies have provided some laboratory rationale for ibrutinib and venetoclax combination 41, 42. Cervantes-Gomez and colleagues performed ex vivo and in vitro studies on residual circulating CLL cells from patients on ibrutinib treatment. They found that venetoclax induced a significant degree of cell death, and concluded that venetoclax is an optimal partner with ibrutinib 41. However, the PB CLL cells were treated as a whole cell population in this study without stromal support. Deng et al. have found that BTK inhibition enhanced mitochondrial BCL2 dependence and pretreatment of CLL cells with BTK inhibitors enhanced the killing by venetoclax 42. These independent studies support the strategy of combining BTK and BCL2 inhibitors as well as ours. However, our study advanced the current knowledge by demonstrating that CLL subpopulations with different proliferative capacities have different sensitivity to ibrutinib and venetoclax, respectively. The effects on cellular subpopulations have never been seen before due to the limitation of the prior experimental systems. 

Overall, our data are consistent with a number of clinical observations. It is learned that ibrutinib is more effective against nodal disease with rapid reduction of lymphadenopathy, while venetoclax is more effective against PB disease 4, 43. Previously, using gene expression microarray, Herishanu and colleagues demonstrated that CLL cells residing in different anatomic quarters have different proliferative capacity. LN CLL cells, in particular, are more proliferative than the PB CLL cells, as evidenced by a higher expression of E2F-target and MYC-target genes and a higher fraction of Ki-67 + cells 44. Putting this together with our laboratory data, the compartmental responses to the BTKi and BCL2i can now be explained by the different proliferative capacities of the CLL subpopulations residing in these different anatomical compartments. 

In addition, our results may contribute to the understanding of several other clinical observations. (1) The complete response rate of ibrutinib monotherapy remains low at 10% for R/R patients and <30% for treatment-naïve patients after a 5-year follow-up 45. According to our current and previous findings [25] [26] [27] 32, this results from little direct cell killing rendered by ibrutinib. On the contrary, tumor lysis syndrome associated with venetoclax can be explained by the direct killing of the resting CLL cells in the periphery. (2) Complete remission with uMRD has been low for ibrutinib or venetoclax monotherapy, especially ibrutinib alone. In contrast, the combination has produced durable responses with high rates of complete remission and uMRD which continuously increases over time 7, 8. This observation was reproduced in our ex vivo system where the BTK and BCL2 inhibitors target distinct subpopulations of CLL cells. Together they leave few tumor cells unscathed. 

Besides proliferation, ibrutinib also plays a key role in disrupting cell adhesion 39, 40, 46. Clinically, when ibrutinib is started, it is widely observed that a rapid reduction in lymphadenopathy is accompanied by a marked increase in circulating lymphocytes. Disruption of tumor cell adherence to nodal stroma is attributed as the cause of this egress. Inhibition of cell proliferation and disruption of cell adhesion are believed to happen in parallel with ibrutinib treatment. 

Integrating clinical and laboratory data, the following tumor cell dynamic can be envisioned when patients are treated with either single drug alone or the drug combination (Fig. 7 ): In LN, with close contact between tumor and stroma, CLL cells are well supported and proliferate; with ibrutinib treatment, adhesion to stromal cells is disrupted and proliferation is halted. Subsequently, CLL cells are released to the periphery where they are also prevented from homing back to LN by the action of ibrutinib on chemokine signaling. In the periphery, without the supporting tumor microenvironment, the cells become non-proliferating/resting and die by neglect over a relatively long period of time; in the presence of venetoclax, however, these resting cells are subjected to active killing, causing tumor lysis syndrome and tumor burden improvement. This proposed dynamic is compatible with current laboratory data and clinical experience but may need further investigational proof. 

Altogether, our study suggests that the combination, with its ability to target both subpopulations of tumor cells, has the greatest potential to minimize or even eradicate MRD at all anatomic sites, which would eventually translate to a reduction in future relapses. In addition, our model or other similar models, may become a useful and practical tool for testing the effectiveness of other drugs or drug combinations, not only on cell viability but also on cell proliferation. Cell adhesion, another critical cellular behavior, may also be evaluated using these models.
section_name: Discussion

section_name: Acknowledgements

section_content: The study is partially funded by a Leukemia and Lymphoma Society TRP grant to Y. L. W.
section_name: Acknowledgements

section_name: Author details

section_content: 1 Fox Chase Cancer Center, Philadelphia, PA, USA. 2 Guangdong Medical University, Dongguan, China. 3 University of Chicago Medicine, Chicago, IL, USA. 4 Health Sciences North Research Institute, Sudbury, ON, Canada. 5 St. Louis University, St Louis, MO, USA. 6 Northwestern University Feinberg School of Medicine, Chicago, IL, USA Author contributions Y. L. W. formed the hypothesis and developed the ideas. P. L., S. W., L. L., and C. A. F. developed the assays, designed and performed the experiments, solved technical problems, and analyzed the data. R. M. performed and interpreted the IGHV mutation assay. W. W. contributed to graph presentation. G. V., J. G., Y. C., Y. T., S. M., R. A. L., and J. P. contributed useful patient materials and patient pathological and treatment information. N. N., M. S., R. M. N., D. A. B., M. A. W., and N. K. and all other authors contributed to useful discussions of the project. All authors participated in the manuscript writing. P. L. and Y. L. W. directed, supervised, and coordinated the project, designed the experiments, analyzed the data, and wrote the manuscript. 

Conflict of interest Y. T., Speaker's bureau for Novartis. S. M., Consultancy for AstraZeneca, Bioverativ, Genentech, Janssen, Kite and Pharmacyclics, Speaker's bureau for AstraZeneca, Janssen and Pharmacyclics, and research funding from Abbvie, AstraZeneca, Beigene, Pharmacyclics, Juno and Novartis. N. K., advisory board for Pharmacyclics, Jessen and Seattle Genetics, and research funding from Bristol Meyer Squibb. Y. L. W., employment and stock ownership at Incyte Research Institute. All other authors declare no relevant conflicts of interest.
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section_name: Publisher's note

section_content: The online version contains supplementary material available at https://doi. org/10. 1038/s41408-021-00429-z.
section_name: Supplementary information