Title: Ossification center
Content:  There are two types of ossification centers – primary and secondary. A primary ossification center is the first area of a bone to start ossifying. It usually appears during prenatal development in the central part of each developing bone. In long bones the primary centers occur in the diaphysis/shaft and in irregular bones the primary centers occur usually in the body of the bone. Most bones have only one primary center (e.g. all long bones except clavicle) but some irregular bones such as the os coxa (hip) and vertebrae have multiple primary centers. A secondary ossification center is the area of ossification that appears after the primary ossification center has already appeared – most of which appear during the postnatal and adolescent years. Most bones have more than one secondary ossification center. In long bones, the secondary centers appear in the epiphyses.
Title: Primary bone
Content:  Primary bone or the primary ossification center is the beginning of the bone building process during the first trimester. Calcificed cartilage is basophilic and new bone being made is more acidophilic. The primary ossification occurs in the diaphysis. In contrast, secondary ossification centers appear later at the epiphyses of the cartilage and develop similarly to the diaphysis.
Title: Humerus
Content:  During embryonic development, the humerus is one of the first structures to ossify, beginning with the first ossification center in the shaft of the bone. Ossification of the humerus occurs predictably in the embryo and fetus, and is therefore used as a fetal biometric measurement when determining gestational age of a fetus. At birth, the neonatal humerus is only ossified in the shaft. The epiphyses are cartilaginous at birth. The medial humeral head develops an ossification center around 4 months of age and the greater tuberosity around 10 months of age. These ossification centers begin to fuse at 3 years of age. The process of ossification is complete by 13 years of age, though the epiphyseal plate (growth plate) persists until skeletal maturity, usually around 17 years of age.
Title: Axis (anatomy)
Content:  The axis is ossified from five primary and two secondary centres. The body and vertebral arch are ossified in the same manner as the corresponding parts in the other vertebrae, viz., one centre for the body, and two for the vertebral arch. The centres for the arch appear about the seventh or eighth week of fetal life, while the centres for the body appear in about the fourth or fifth month. The dens, or odontoid process, consist originally of a continuation upward of the cartilaginous mass, in which the lower part of the body is formed. During about the sixth month of fetal life, two centres make their appearance in the base of this process: they are placed laterally, and join before birth to form a conical bilobed mass deeply 
Title: Ossification center
Content:  An ossification center is a point where ossification of the cartilage begins. The first step in ossification is that the cartilage cells at this point enlarge and arrange themselves in rows. The matrix in which they are imbedded increases in quantity, so that the cells become further separated from each other. A deposit of calcareous material now takes place in this matrix, between the rows of cells, so that they become separated from each other by longitudinal columns of calcified matrix, presenting a granular and opaque appearance. Here and there the matrix between two cells of the same row also becomes calcified, and transverse bars of calcified substance stretch across from one calcareous column to another. Thus there are longitudinal groups of the cartilage cells enclosed in oblong cavities, the walls of which are formed of calcified matrix which cuts off all nutrition from the cells; the cells, in consequence, atrophy, leaving spaces called the primary areolæ.
Title: Ossification
Content:  The canal of the nutrient foramen is directed away from more active end of bone when one end grows more than the other. When bone grows at same rate at both ends, the nutrient artery is perpendicular to the bone. Most other bones (e.g. vertebrae) also have primary ossification centers, and bone is laid down in a similar manner. Secondary centers The secondary centers generally appear at the epiphysis. Secondary ossification mostly occurs after birth (except for distal femur and proximal tibia which occurs during 9th month of fetal development). The epiphyseal arteries and osteogenic cells invade the epiphysis, depositing osteoclasts and osteoblasts which erode the cartilage and build bone, respectively. This occurs at both ends of long bones but only one end of digits and ribs.
Title: Rib
Content:  Early in the developing embryo, somites form and soon subdivide into three mesodermal components – the myotome, dermatome, and the sclerotome. The vertebrae and ribs develop from the sclerotomes. During the fourth week (fertilization age) costal processes have formed on the vertebral bodies. These processes are small, lateral protrusions of mesenchyme that develop in association with the vertebral arches. During the fifth week the costal processes on the thoracic vertebrae become longer to form the ribs. In the sixth week, the costovertebral joints begin to develop and separate the ribs from the vertebrae. The first seven pairs of ribs, the true ribs join at the front to the sternal bars. By the fetal stage the sternal bars have completely fused. The ribs begin as cartilage that later ossifies – a process called endochondral ossification. Primary ossification centers are located near the angle of each rib, and ossification continues in the direction away from the head and neck. During adolescence secondary ossification centers are formed in the tubercles and heads of the ribs.
Title: Radius (bone)
Content:  The radius is ossified from three centers: one for the body, and one for each extremity. That for the body makes its appearance near the center of the bone, during the eighth week of fetal life. Ossification commences in the lower end between 9 and 26 months of age. The ossification center for the upper end appears by the fifth year. The upper epiphysis fuses with the body at the age of seventeen or eighteen years, the lower about the age of twenty. An additional center sometimes found in the radial tuberosity, appears about the fourteenth or fifteenth year.
Title: Ossification
Content:  Endochondral ossification is the formation of long bones and other bones. This requires a hyaline cartilage precursor. There are two centers of ossification for endochondral ossification. The primary center In long bones, bone tissue first appears in the diaphysis (middle of shaft). Chondrocytes multiply and form trebeculae. Cartilage is progressively eroded and replaced by hardened bone, extending towards the epiphysis. A perichondrium layer surrounding the cartilage forms the periosteum, which generates sperm cells that then go on to make a collar that encircles the outside of the bone and remodels the medullary cavity on the inside. The nutrient artery enters via the nutrient foramen from a small opening in the diaphysis. It invades the primary center of ossification, bringing osteogenic cells (osteoblasts on the outside, osteoclasts on the 
Title: Tibia
Content:  The tibia is ossified from three centers; a primary center for the diaphysis (shaft) and a secondary center for each epiphysis (extremity). Ossification begins in the center of the body, about the seventh week of fetal life, and gradually extends toward the extremities. The center for the upper epiphysis appears before or shortly after birth at close to 34 weeks gestation; it is flattened in form, and has a thin tongue-shaped process in front, which forms the tuberosity; that for the lower epiphysis appears in the second year. The lower epiphysis fuses with the tibial shaft at about the eighteenth, and the upper one fuses about the twentieth year. Two additional centers occasionally exist, one for the tongue-shaped process of the upper epiphysis, which forms the tuberosity, and one for the medial malleolus.

Question: when do primary ossification centers appear in an embryo?
Answer: prenatal development.