RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: 02rc
Target: Ovarian cancer tissues (OVCAR-3, National Cancer Institute #0507709, 104 cells per test)
Type of nucleic acid: ssDNA
Sequence: 5'GGCAGGAAGACAAACACCCCCTCGGTTCCACCCCGTCATGATTTTCCGTAATTTGATGGTCTGTGGTGCTGT3'
Sequence length: 72
GC content: 0.5277777777777778
Affinity: Kd: 2.9 ± 0.8 nM
Pool type: 5′-GGCAGGAAGACAAACA-N40-TGGTCTGTGGTGCTGT-3′
Pool random region: 40
Binding buffer/conditions: 50 ml of Dulbecco's phosphate-buffered saline (DPBS; Sigma, USA), 0.225 g of glucose, 250 μl of 1 M MgCl2, and 5 mg of yeast transfer RNA (tRNA; Invitrogen, USA)
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " Given the comparable specificity to their much more expensive antibody counterparts, these aptamers, when used in conjunction with the developed microfluidic system, may be used to diagnose ovarian cancer in its earliest stages. Automated, microfluidic, tissue-SELEX system developed herein is a promising tool for rapid screening cancer tissue-specific aptamers."
Reference (2019, Biomicrofluidics ): Liu, W. T., Lee, W. B., Tsai, Y. C., Chuang, Y. J., Hsu, K. F., & Lee, G. B. (2019). An automated microfluidic system for selection of aptamer probes against ovarian cancer tissues. Biomicrofluidics, 13(1), 014114. https://doi.org/10.1063/1.5085133
Aptamer: H02
Target: Integrin α5β1-expressing cells, Human & Chinese Hamster
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5'GGUUACCAGCCUUCACUGCGGACGGACAGAGAGUGCAACCUGCCGUGCCGCACCACGGUCGGUCACAC3'
Sequence length: 68
GC content: 0.6470588235294118
Affinity: Kd: 72 ±11 nM for integrin α5β1
Pool type: 5′-GTGTGACCGACCGTGGTGC-N30-GCAGTGAAGGCTGGTAACC-3′
Pool random region: 30
Binding buffer/conditions: 1 mM MgCl2 and 0.5 mM CaCl2 in PBS (pH 7.4)
Divalent salt: MgCl/CaCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamer H02 is able to differentiate, in cyto- and histofluorescence assays, glioblastoma cell lines, and tissues from patient-derived tumor xenografts according to their α5 expression levels. Aptamer H02 is therefore an interesting tool for glioblastoma tumor characterization. Aptamer H02 is an effective molecular probe for labeling histological tissue sections and detection of the α5β1 biomarker on tumor cells. Internalized, an aptamer targeting integrin α5β1 might open roads for α5β1-specific therapeutic payload delivery. Linked to a cytotoxic agent, an aptamer to integrin α5β1 could serve as a carrier for targeted therapeutic delivery."
Additional information: 2′-fluoropyrimidine modification, End-labeled with a single cyanine 5 fluorescent dye
This SELEX combines three rounds of protein-SELEX surrounded by 15 rounds of cell-SELEX on two different cell lines genetically modified to overexpress integrin α5, the human GBM U87MG cell line, and the Chinese hamster ovaries CHO-B2 cell line.
Reference (2019, Mol Ther Nucleic Acids ): Fechter, P., Cruz Da Silva, E., Mercier, M. C., Noulet, F., Etienne-Seloum, N., Guenot, D., Lehmann, M., Vauchelles, R., Martin, S., Lelong-Rebel, I., Ray, A. M., Seguin, C., Dontenwill, M., & Choulier, L. (2019). RNA Aptamers Targeting Integrin α5β1 as Probes for Cyto- and Histofluorescence in Glioblastoma. Molecular therapy. Nucleic acids, 17, 63–77. https://doi.org/10.1016/j.omtn.2019.05.006
Aptamer: Apt03 > TH1N1
Target: Influenza virus subtype H1N1
Type of nucleic acid: ssDNA
Sequence: 5'AGCAGCACAGAGGTCAGATGTAGGTCGTACTCTGGCGGCCTGTTTGGCCCTATCGCTGCTACCGTGAA3'
Sequence length: 68
GC content: 0.5735294117647058
Affinity: Kd: 0.082 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N30-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 30
Binding buffer/conditions: PBS buffer (pH 7.4), 100 mM NaCl, 5 mM KCl, 2 mM MgCl2, 1 mM CaCl2
Divalent salt: MgCl/CaCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Construction of an influenza virus subtype-specific and portable detector: DEPSOR-mode electrochemical sensing method (or Apta-DEPSOR) readily evaluatesthe specific binding of aptamers. On the whole, a powerful approach for rapidly detecting various influenza subtypes with high sensitivity is presented here, and it addresses several theoretical considerations. By making the H1N1-specific DNA aptamer a sensor unit of the DEPSOR electrochemical detector, an influenza virus subtype-specific and portable detector was readily constructed, indicating how close it is to the field application goal."
Reference (2019, Sci Rep ): Kushwaha, A., Takamura, Y., Nishigaki, K., & Biyani, M. (2019). Competitive non-SELEX for the selective and rapid enrichment of DNA aptamers and its use in electrochemical aptasensor. Scientific Reports, 9(1), not reported. https://doi.org/10.1038/s41598-019-43187-1
Aptamer: Apt04 > TH1N1
Target: Influenza virus subtype H1N1
Type of nucleic acid: ssDNA
Sequence: 5'AGCAGCACAGAGGTCAGATGTGTGCGTGCTTGGGGTATAGTCGGGTCGGCCTATCGCTGCTACCGTGAA3'
Sequence length: 69
GC content: 0.5797101449275363
Affinity: Kd: 1.6 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N30-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 30
Binding buffer/conditions: PBS buffer (pH 7.4), 100 mM NaCl, 5 mM KCl, 2 mM MgCl2, 1 mM CaCl2
Divalent salt: MgCl/CaCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Construction of an influenza virus subtype-specific and portable detector: DEPSOR-mode electrochemical sensing method (or Apta-DEPSOR) readily evaluatesthe specific binding of aptamers. On the whole, a powerful approach for rapidly detecting various influenza subtypes with high sensitivity is presented here, and it addresses several theoretical considerations. By making the H1N1-specific DNA aptamer a sensor unit of the DEPSOR electrochemical detector, an influenza virus subtype-specific and portable detector was readily constructed, indicating how close it is to the field application goal."
Reference (2019, Sci Rep ): Kushwaha, A., Takamura, Y., Nishigaki, K., & Biyani, M. (2019). Competitive non-SELEX for the selective and rapid enrichment of DNA aptamers and its use in electrochemical aptasensor. Scientific Reports, 9(1), not reported. https://doi.org/10.1038/s41598-019-43187-1
Aptamer: Apt02 > TH1N1
Target: Influenza virus subtype H1N1
Type of nucleic acid: ssDNA
Sequence: 5'AGCAGCACAGAGGTCAGATGAGGTGATGAGATTTGTACCTCTCGCGGCACCCTATCGCTGCTACCGTGAA3'
Sequence length: 70
GC content: 0.5428571428571428
Affinity: Kd: 57 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N30-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 30
Binding buffer/conditions: PBS buffer (pH 7.4), 100 mM NaCl, 5 mM KCl, 2 mM MgCl2, 1 mM CaCl2
Divalent salt: MgCl/CaCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Construction of an influenza virus subtype-specific and portable detector: DEPSOR-mode electrochemical sensing method (or Apta-DEPSOR) readily evaluatesthe specific binding of aptamers. On the whole, a powerful approach for rapidly detecting various influenza subtypes with high sensitivity is presented here, and it addresses several theoretical considerations. By making the H1N1-specific DNA aptamer a sensor unit of the DEPSOR electrochemical detector, an influenza virus subtype-specific and portable detector was readily constructed, indicating how close it is to the field application goal."
Reference (2019, Sci Rep ): Kushwaha, A., Takamura, Y., Nishigaki, K., & Biyani, M. (2019). Competitive non-SELEX for the selective and rapid enrichment of DNA aptamers and its use in electrochemical aptasensor. Scientific Reports, 9(1), not reported. https://doi.org/10.1038/s41598-019-43187-1
Aptamer: Apt01 > TH1N1
Target: Influenza virus subtype H1N1
Type of nucleic acid: ssDNA
Sequence: 5'AGCAGCACAGAGGTCAGATGATTGGATCGTGACGGTTGTTGGGGCTCCGCCTATCGCTGCTACCGTGAA3'
Sequence length: 69
GC content: 0.5652173913043478
Affinity: Kd: 35000 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N30-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 30
Binding buffer/conditions: PBS buffer (pH 7.4), 100 mM NaCl, 5 mM KCl, 2 mM MgCl2, 1 mM CaCl2
Divalent salt: MgCl/CaCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Construction of an influenza virus subtype-specific and portable detector: DEPSOR-mode electrochemical sensing method (or Apta-DEPSOR) readily evaluatesthe specific binding of aptamers. On the whole, a powerful approach for rapidly detecting various influenza subtypes with high sensitivity is presented here, and it addresses several theoretical considerations. By making the H1N1-specific DNA aptamer a sensor unit of the DEPSOR electrochemical detector, an influenza virus subtype-specific and portable detector was readily constructed, indicating how close it is to the field application goal."
Reference (2019, Sci Rep ): Kushwaha, A., Takamura, Y., Nishigaki, K., & Biyani, M. (2019). Competitive non-SELEX for the selective and rapid enrichment of DNA aptamers and its use in electrochemical aptasensor. Scientific Reports, 9(1), not reported. https://doi.org/10.1038/s41598-019-43187-1
Aptamer: Apt04 > TH3N2
Target: Influenza virus subtype H3N2
Type of nucleic acid: ssDNA
Sequence: 5'AGCAGCACAGAGGTCAGATGTCTGCAGCGTGCAGGGCTGTGTGCTTACCCCCTATCGCTGCTACCGTGAA3'
Sequence length: 70
GC content: 0.5857142857142857
Affinity: Kd: 0.088 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N30-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 30
Binding buffer/conditions: PBS buffer (pH 7.4), 100 mM NaCl, 5 mM KCl, 2 mM MgCl2, 1 mM CaCl2
Divalent salt: MgCl/CaCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Construction of an influenza virus subtype-specific and portable detector: DEPSOR-mode electrochemical sensing method (or Apta-DEPSOR) readily evaluatesthe specific binding of aptamers. On the whole, a powerful approach for rapidly detecting various influenza subtypes with high sensitivity is presented here, and it addresses several theoretical considerations. By making the H1N1-specific DNA aptamer a sensor unit of the DEPSOR electrochemical detector, an influenza virus subtype-specific and portable detector was readily constructed, indicating how close it is to the field application goal."
Reference (2019, Sci Rep ): Kushwaha, A., Takamura, Y., Nishigaki, K., & Biyani, M. (2019). Competitive non-SELEX for the selective and rapid enrichment of DNA aptamers and its use in electrochemical aptasensor. Scientific Reports, 9(1), not reported. https://doi.org/10.1038/s41598-019-43187-1
Aptamer: Apt01 > TH3N2
Target: Influenza virus subtype H3N2
Type of nucleic acid: ssDNA
Sequence: 5'AGCAGCACAGAGGTCAGATGCTAGCCGTGAGCGTGGTGAGCTCGGTTGACCCTATCGCTGCTACCGTGAA3'
Sequence length: 70
GC content: 0.5857142857142857
Affinity: Kd: 0.14 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N30-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 30
Binding buffer/conditions: PBS buffer (pH 7.4), 100 mM NaCl, 5 mM KCl, 2 mM MgCl2, 1 mM CaCl2
Divalent salt: MgCl/CaCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Construction of an influenza virus subtype-specific and portable detector: DEPSOR-mode electrochemical sensing method (or Apta-DEPSOR) readily evaluatesthe specific binding of aptamers. On the whole, a powerful approach for rapidly detecting various influenza subtypes with high sensitivity is presented here, and it addresses several theoretical considerations. By making the H1N1-specific DNA aptamer a sensor unit of the DEPSOR electrochemical detector, an influenza virus subtype-specific and portable detector was readily constructed, indicating how close it is to the field application goal."
Reference (2019, Sci Rep ): Kushwaha, A., Takamura, Y., Nishigaki, K., & Biyani, M. (2019). Competitive non-SELEX for the selective and rapid enrichment of DNA aptamers and its use in electrochemical aptasensor. Scientific Reports, 9(1), not reported. https://doi.org/10.1038/s41598-019-43187-1
Aptamer: Apt03 > TH3N2
Target: Influenza virus subtype H3N2
Type of nucleic acid: ssDNA
Sequence: 5'AGCAGCACAGAGGTCAGATGGCGCGGGCGGTGCGTCGGTGTCCCGCTGGCCTATCGCTGCTACCGTGAA3'
Sequence length: 69
GC content: 0.6666666666666666
Affinity: Kd: 0.60 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N30-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 30
Binding buffer/conditions: PBS buffer (pH 7.4), 100 mM NaCl, 5 mM KCl, 2 mM MgCl2, 1 mM CaCl2
Divalent salt: MgCl/CaCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Construction of an influenza virus subtype-specific and portable detector: DEPSOR-mode electrochemical sensing method (or Apta-DEPSOR) readily evaluatesthe specific binding of aptamers. On the whole, a powerful approach for rapidly detecting various influenza subtypes with high sensitivity is presented here, and it addresses several theoretical considerations. By making the H1N1-specific DNA aptamer a sensor unit of the DEPSOR electrochemical detector, an influenza virus subtype-specific and portable detector was readily constructed, indicating how close it is to the field application goal."
Reference (2019, Sci Rep ): Kushwaha, A., Takamura, Y., Nishigaki, K., & Biyani, M. (2019). Competitive non-SELEX for the selective and rapid enrichment of DNA aptamers and its use in electrochemical aptasensor. Scientific Reports, 9(1), not reported. https://doi.org/10.1038/s41598-019-43187-1
Aptamer: Apt02 > TH3N2
Target: Influenza virus subtype H3N2
Type of nucleic acid: ssDNA
Sequence: 5'AGCAGCACAGAGGTCAGATGGTGGTTGTTTTGGGCGAAGTGGCCATGGTCCCTATCGCTGCTACCGTGAA3'
Sequence length: 70
GC content: 0.5571428571428572
Affinity: Kd: 1.7 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N30-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 30
Binding buffer/conditions: PBS buffer (pH 7.4), 100 mM NaCl, 5 mM KCl, 2 mM MgCl2, 1 mM CaCl2
Divalent salt: MgCl/CaCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Construction of an influenza virus subtype-specific and portable detector: DEPSOR-mode electrochemical sensing method (or Apta-DEPSOR) readily evaluatesthe specific binding of aptamers. On the whole, a powerful approach for rapidly detecting various influenza subtypes with high sensitivity is presented here, and it addresses several theoretical considerations. By making the H1N1-specific DNA aptamer a sensor unit of the DEPSOR electrochemical detector, an influenza virus subtype-specific and portable detector was readily constructed, indicating how close it is to the field application goal."
Reference (2019, Sci Rep ): Kushwaha, A., Takamura, Y., Nishigaki, K., & Biyani, M. (2019). Competitive non-SELEX for the selective and rapid enrichment of DNA aptamers and its use in electrochemical aptasensor. Scientific Reports, 9(1), not reported. https://doi.org/10.1038/s41598-019-43187-1
Aptamer: TC-6d (Truncated TC-6)
Target: Papillary thyroid carcinoma cell line TPC-2
Type of nucleic acid: ssDNA
Sequence: 5′ACTGACTACCTACCACCACACCACATCTCCAGCCTCAACGTCGCTTTGTCGAAGTCAGTC3′
Sequence length: 60
GC content: 0.5333333333333333
Affinity: Kd: 39.20 ± 8.20 nM
Pool type: 5′-ATCCATTGCCACTGACTACC-N40-GAAGTCAGTCGGTCGTTAGT-3′
Pool random region: 40
Binding buffer/conditions: 5 mmol/L MgCl2 and 4.5 g/L glucose in Dulbecco's phosphate-buffered saline, 0.1 mg/mL yeast tRNA and 1 mg/L bovine serum albumin (BSA)
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: " TC-6 is a potential candidate for developing novel tools for diagnosis and targeted therapy of PTC. The truncated aptamer TC-6d could maintain its binding capability and recognize PTC in a manner similar to that of full-length aptamer. Our results demonstrated that TC-6 had high affinity to PTC in cell lines, tissue slides and tumor-bearing mice. Therefore, TC-6 shows promise in the diagnosis of thyroid malignant diseases and targeted therapy of PTC."
Reference (2019, Anal Chem ): Zhong, W., Pu, Y., Tan, W., Liu, J., Liao, J., Liu, B., Chen, K., Yu, B., Hu, Y., Deng, Y., Zhang, J., & Liu, H. (2019). Identification and Application of an Aptamer Targeting Papillary Thyroid Carcinoma Using Tissue-SELEX. Analytical chemistry, 91(13), 8289–8297. https://doi.org/10.1021/acs.analchem.9b01000
Aptamer: APTL-1
Target: Norovirus P particles (GG2.4P), Human
Type of nucleic acid: ssDNA
Sequence: 5′CGATCAAACGTTCAAGCGGGGCCCGGAGGCGTGACTTGGACAGGCAGGCGTTACGATGCATCCCGCAAATGACGCATGA3′
Sequence length: 79
GC content: 0.5949367088607594
Affinity: Kd: 148.13 ± 6.53 nM
Pool type: 5′-CGATCAAACGTTCAAGCGGG-N40-ATCCCGCAAATGACGCATGA-3′
Pool random region: 40
Binding buffer/conditions: 1 × BB, 50 mM Tris-HCl pH 7.4, 5 mM KCl, 100 mM NaCl, and 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Human noroviruses (HuNoVs) is the primary non-bacterial pathogen causing acute gastroenteritis worldwide. Molecular approaches have been mainly used for detection of HuNoVs. Aptamer-based assay has been also applied for detection of HuNoVs through affinity binding of viral capsid. The selected aptamer APTL-1 was comparable to PGM and slightly superior to the reported APTM6-2 aptamer for detection of HuNoVs from clinical samples. The results demonstrated that this in situ target-capture approach for aptamer selection is practicable."
Reference (2019, Talanta ): Liu, D., Zhang, Z., Yin, Y., Jia, F., Wu, Q., Tian, P., & Wang, D. (2019). Development and evaluation of a novel in situ target-capture approach for aptamer selection of human noroviruses. Talanta, 193, 199–205. https://doi.org/10.1016/j.talanta.2018.09.084
Aptamer: APTL-6
Target: Norovirus P particles (GG2.4P), Human
Type of nucleic acid: ssDNA
Sequence: 5′CGATCAAACGTTCAAGCGGGGCCCAAGCGGCGTGGTCTGAAGTCCTGGCTGACTGCGGCGATCCCGCAAATGACGCATGA3′
Sequence length: 80
GC content: 0.6125
Affinity: Kd: 183.30 ± 56.27 nM
Pool type: 5′-CGATCAAACGTTCAAGCGGG-N40-ATCCCGCAAATGACGCATGA-3′
Pool random region: 40
Binding buffer/conditions: 1 × BB, 50 mM Tris-HCl pH 7.4, 5 mM KCl, 100 mM NaCl, and 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Human noroviruses (HuNoVs) is the primary non-bacterial pathogen causing acute gastroenteritis worldwide. Molecular approaches have been mainly used for detection of HuNoVs. Aptamer-based assay has been also applied for detection of HuNoVs through affinity binding of viral capsid. The selected aptamer APTL-1 was comparable to PGM and slightly superior to the reported APTM6-2 aptamer for detection of HuNoVs from clinical samples. The results demonstrated that this in situ target-capture approach for aptamer selection is practicable."
Reference (2019, Talanta ): Liu, D., Zhang, Z., Yin, Y., Jia, F., Wu, Q., Tian, P., & Wang, D. (2019). Development and evaluation of a novel in situ target-capture approach for aptamer selection of human noroviruses. Talanta, 193, 199–205. https://doi.org/10.1016/j.talanta.2018.09.084
Aptamer: NPE-01
Target: Nonylphenol ethoxylate (NPE)
Type of nucleic acid: ssDNA
Sequence: 5′ATGCGGATCCCGCGCCGGGCCACAGGGTGCGCGAAGCTTGCGC3′
Sequence length: 43
GC content: 0.7441860465116279
Affinity: Kd: 100.9 ± 13.2 nM
Pool type: 5′‐ATGCGGATCCCGCGC‐N30‐GCGCGAAGCTTGCGC‐3′
Pool random region: 30
Binding buffer/conditions: Tris‐HCl 125 mM, NaCl 500 mM, KCl 125 mM, MgCl 12.5 mM, DMSO 25% (v/v)
Divalent salt: MgCl
Buffer type: Tris Buffers
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Endocrine-disrupting chemicals are a major public health problem throughout the world. In the human body, these compounds functionalize the same as sexual hormones, inducing precocious puberty, gynecomastia, etc. In this study, a nonylphenol ethoxylate (NPE)-specific aptamer was selected by reduced graphene oxide-systematic evolution of ligands by exponential enrichment. From these results, we expect that this aptamer could be applied to an on-site detection system for NPE in industrial sites or domestic fields."
Reference (2019, J Mol Recognit ): Kim, A. R., Ha, N. R., Jung, I. P., Kim, S. H., & Yoon, M. Y. (2019). Development of a ssDNA aptamer system with reduced graphene oxide (rGO) to detect nonylphenol ethoxylate in domestic detergent. Journal of molecular recognition : JMR, 32(3), e2764. https://doi.org/10.1002/jmr.2764
Aptamer: APT 1
Target: Mixed lineage leukemia protein 1 (MLL1)
Type of nucleic acid: ssRNA
Sequence: 5′GGCUCGAGGAACGUACAGAGGGUGGAGAGUGGGUGGAAGCUUACGGUACCUAGC3′
Sequence length: 54
GC content: 0.5925925925925926
Affinity: Not reported
Pool type: 5′-CGACTCACTATAGGCTCGAGG-N25-GGAAGCTTACGGTACCTAGC-3′
Pool random region: 25
Binding buffer/conditions: NETN buffer media (20 mM Tris pH 8.0, 100 mM NaCl, 1 mM EDTA, and 0.5% NP-40)
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: " We suggest that MLL1-specific aptamers could be further developed for useful applications in various areas, including as therapeutic or diagnostic agents in the biomedical field. APT1 and APT2 are prominent candidates for development of diagnosis or therapy in MLL1-related diseases. First, by developing these aptamers as chemical probes, the function and biological properties can be examined. Second, these aptamers can be tested whether these are used as a therapy related to aberrant regulation of methylase activity of MLL1. In the future, the study of these aptamers will provide deeper insights and more therapeutic potential."
Reference (2019, Biomol Ther (Seoul) ): Ul-Haq, A., Jin, M. L., Jeong, K. W., Kim, H. M., & Chun, K. H. (2019). Isolation of MLL1 Inhibitory RNA Aptamers. Biomolecules & therapeutics, 27(2), 201–209. https://doi.org/10.4062/biomolther.2018.157