RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: DNA04
Target: Palladium ion
Type of nucleic acid: ssDNA
Sequence: 5′CGTACGGAATTCGCTAGCCCCCGCTGCCCGTGTTCCGTCCTCCCTTGCTGTGTGTGCGGGATCCGAGCTCCACGTG3′
Sequence length: 76
GC content: 0.6578947368421053
Affinity: Not reported
Pool type: 5′-CGTACGGAATTCGCTAGC-N40-GGATCCGAGCTCCACGTG-3′
Pool random region: 40
Binding buffer/conditions: HEPES (0.1 M) at pH 8.02
Buffer type: Other Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " A new aptamer selection method using graphene oxide (GO)-adsorbed nanoparticles (GO-adsorbed NPs) was employed for specific fishing of palladium ion. Results of the present study suggest that future modification of DNA01 may improve palladium ion-binding ability, leading to economic recovery of palladium from water solution."
Reference (2015, Bioorg Med Chem Lett ): Cho, Y. S., Lee, E. J., Lee, G. H., & Hah, S. S. (2015). Aptamer selection for fishing of palladium ion using graphene oxide-adsorbed nanoparticles. Bioorganic & medicinal chemistry letters, 25(23), 5536–5539. https://doi.org/10.1016/j.bmcl.2015.10.056
Aptamer: AP244
Target: Alpha-fetoprotein (AFP)
Type of nucleic acid: ssDNA
Sequence: 5′GTGACGCTCCTAACGCTGACGTGACGCTCCTAACGCTGACGGCCCGTCAATCCCCGCGTATCCATTGGTCCTTTACCTGTCCGTCCGAACCAATCCCTGTCCGTCCGAACCAATC3′
Sequence length: 115
GC content: 0.591304347826087
Affinity: Kd: 4.8 μM
Pool type: 5′-GTGACGCTCCTAACGCTGAC-N35-CCTGTCCGTCCGAACCAATC-3′
Pool random region: 35
Binding buffer/conditions: CE Buffer (30 mM NaH2PO4) at a pH of 7.5.
Buffer type: PBS/phosphate buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: " Alpha-fetoprotein (AFP) is a liver cancer associated protein and has long been utilized as a serum tumor biomarker of disease progression. In this study, AFP-bound ssDNA aptamers were screened and identified using capillary electrophoresis (CE) SELEX technology. Our results indicate that CE-SELEX technology is an efficient method to screen specific protein-bound ssDNA, and AP273 could be used as an agent in AFP-based staining, diagnosis and therapy, although more works are still needed."
Reference (2015, Sci Rep ): Dong, L., Tan, Q., Ye, W., Liu, D., Chen, H., Hu, H., Wen, D., Liu, Y., Cao, Y., Kang, J., Fan, J., Guo, W., & Wu, W. (2015). Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein Using CE-SELEX. Scientific reports, 5, 15552. https://doi.org/10.1038/srep15552
Aptamer: AP206
Target: Alpha-fetoprotein (AFP)
Type of nucleic acid: ssDNA
Sequence: 5′GTGACGCTCCTAACGCTGACGTGACGCTCCTAACGCTGACTTTTAATTCTTGTTTATTGGTATATCCGCCTCTGACCTGTCCGTCCGAACCAATCCCTGTCCGTCCGAACCAATC3′
Sequence length: 115
GC content: 0.5130434782608696
Affinity: Not reported
Pool type: 5′-GTGACGCTCCTAACGCTGAC-N35-CCTGTCCGTCCGAACCAATC-3′
Pool random region: 35
Binding buffer/conditions: CE Buffer (30 mM NaH2PO4) at a pH of 7.5.
Buffer type: PBS/phosphate buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: " Alpha-fetoprotein (AFP) is a liver cancer associated protein and has long been utilized as a serum tumor biomarker of disease progression. In this study, AFP-bound ssDNA aptamers were screened and identified using capillary electrophoresis (CE) SELEX technology. Our results indicate that CE-SELEX technology is an efficient method to screen specific protein-bound ssDNA, and AP273 could be used as an agent in AFP-based staining, diagnosis and therapy, although more works are still needed."
Reference (2015, Sci Rep ): Dong, L., Tan, Q., Ye, W., Liu, D., Chen, H., Hu, H., Wen, D., Liu, Y., Cao, Y., Kang, J., Fan, J., Guo, W., & Wu, W. (2015). Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein Using CE-SELEX. Scientific reports, 5, 15552. https://doi.org/10.1038/srep15552
Aptamer: AP250
Target: Alpha-fetoprotein (AFP)
Type of nucleic acid: ssDNA
Sequence: 5′GTGACGCTCCTAACGCTGACGTGACGCTCCTAACGCTGACACAGTTGCTAAGAGCCGCCTCTGAACCTATCGAGGCCTGTCCGTCCGAACCAATCCCTGTCCGTCCGAACCAATC3′
Sequence length: 115
GC content: 0.5826086956521739
Affinity: Not reported
Pool type: 5′-GTGACGCTCCTAACGCTGAC-N35-CCTGTCCGTCCGAACCAATC-3′
Pool random region: 35
Binding buffer/conditions: CE Buffer (30 mM NaH2PO4) at a pH of 7.5.
Buffer type: PBS/phosphate buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: " Alpha-fetoprotein (AFP) is a liver cancer associated protein and has long been utilized as a serum tumor biomarker of disease progression. In this study, AFP-bound ssDNA aptamers were screened and identified using capillary electrophoresis (CE) SELEX technology. Our results indicate that CE-SELEX technology is an efficient method to screen specific protein-bound ssDNA, and AP273 could be used as an agent in AFP-based staining, diagnosis and therapy, although more works are still needed."
Reference (2015, Sci Rep ): Dong, L., Tan, Q., Ye, W., Liu, D., Chen, H., Hu, H., Wen, D., Liu, Y., Cao, Y., Kang, J., Fan, J., Guo, W., & Wu, W. (2015). Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein Using CE-SELEX. Scientific reports, 5, 15552. https://doi.org/10.1038/srep15552
Aptamer: AP211
Target: Alpha-fetoprotein (AFP)
Type of nucleic acid: ssDNA
Sequence: 5′GTGACGCTCCTAACGCTGACGTGACGCTCCTAACGCTGACTTTTTGGGAATGCGTATGTATGCTCGCCACGTACGCCTGTCCGTCCGAACCAATCCCTGTCCGTCCGAACCAATC3′
Sequence length: 115
GC content: 0.5652173913043478
Affinity: Not reported
Pool type: 5′-GTGACGCTCCTAACGCTGAC-N35-CCTGTCCGTCCGAACCAATC-3′
Pool random region: 35
Binding buffer/conditions: CE Buffer (30 mM NaH2PO4) at a pH of 7.5.
Buffer type: PBS/phosphate buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: " Alpha-fetoprotein (AFP) is a liver cancer associated protein and has long been utilized as a serum tumor biomarker of disease progression. In this study, AFP-bound ssDNA aptamers were screened and identified using capillary electrophoresis (CE) SELEX technology. Our results indicate that CE-SELEX technology is an efficient method to screen specific protein-bound ssDNA, and AP273 could be used as an agent in AFP-based staining, diagnosis and therapy, although more works are still needed."
Reference (2015, Sci Rep ): Dong, L., Tan, Q., Ye, W., Liu, D., Chen, H., Hu, H., Wen, D., Liu, Y., Cao, Y., Kang, J., Fan, J., Guo, W., & Wu, W. (2015). Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein Using CE-SELEX. Scientific reports, 5, 15552. https://doi.org/10.1038/srep15552
Aptamer: SEB 2
Target: Staphylococcal enterotoxin B (SEB)
Type of nucleic acid: ssDNA
Sequence: 5'ATAGGAGTCACGACGACCAGAAAATGCTATGATGCTAGTAGCAGTAGCGTAGCAGTCGAATTATGTGCGTCTACCTCTTGACTAAT3'
Sequence length: 86
GC content: 0.4418604651162791
Affinity: Not reported
Pool type: 5'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'
Pool random region: 40
Binding buffer/conditions: binding buffer (5 mM Tris–HCl with 1 M NaCl, pH 7.5)
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: 28 kDa
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " In the present study, we introduce a novel hybrid sandwich-ALISA employing chicken IgY and ssDNA aptamers for the detection of staphylococcal enterotoxin B (SEB). To evaluate the reliability and ease of access, developed method was evaluated onto several food and clinical samples originated from India were undertaken and results of the developed method showed very promising for detection of SEB in tested samples."
Reference (2015, Sci Rep ): Mudili, V., Makam, S. S., Sundararaj, N., Siddaiah, C., Gupta, V. K., & Rao, P. (2015). A novel IgY-Aptamer hybrid system for cost-effective detection of SEB and its evaluation on food and clinical samples. Scientific reports, 5, 15151. https://doi.org/10.1038/srep15151 (Retraction published Sci Rep. 2022 Jun 29;12(1):10939)
Aptamer: QA-92
Target: Soft-shelled turtle iridovirus (STIV)
Type of nucleic acid: ssDNA
Sequence: 5'GACGCTTACTCAGGTGTGACTCGCGGGTGCCTTAAAGTGTAGGTGGGAGGGGGATGATTATGTACGATCAAGTCGAAGGACGCAGAGAAGTCTC3'
Sequence length: 94
GC content: 0.5319148936170213
Affinity: Kd: 71.2 nM
Pool type: 5'-GACGCTTACTCAGGTGTGACTCG-N50-CGAAGGACGCAGAGAAGTCTC-3'
Pool random region: 50
Binding buffer/conditions: 100 mM NaCl, 2.5 mM MgCl2, 20 mM Tris–HCl (pH 7.5)
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Drug Delivery: " Soft-shelled turtle iridovirus (STIV) causes severe systemic disease in farmed soft-shelled turtles (Trionyx sinensis). We generated DNA aptamers that bound STIV with a high level of specificity, providing an alternative means for investigating STIV pathogenesis, drug development, and medical therapies for STIV infection. These DNA aptamers may thus be suitable antiviral candidates for the control of STIV infections."
Reference (2015, BMC Vet Res ): Li, P., Zhou, L., Yu, Y., Yang, M., Ni, S., Wei, S., & Qin, Q. (2015). Characterization of DNA aptamers generated against the soft-shelled turtle iridovirus with antiviral effects. BMC Veterinary Research, 11.  https://doi.org/10.1186/s12917-015-0559-6
Aptamer: QA-9
Target: Soft-shelled turtle iridovirus (STIV)
Type of nucleic acid: ssDNA
Sequence: 5'GACGCTTACTCAGGTGTGACTCGGAAGGTGGAGCGGTTAGGATACTAGCGAATGAAGACACGTTAGCTGATCTCGAAGGACGCAGAGAAGTCTC3'
Sequence length: 94
GC content: 0.5212765957446809
Affinity: Kd: 77.5 nM
Pool type: 5'-GACGCTTACTCAGGTGTGACTCG-N50-CGAAGGACGCAGAGAAGTCTC-3'
Pool random region: 50
Binding buffer/conditions: 100 mM NaCl, 2.5 mM MgCl2, 20 mM Tris–HCl (pH 7.5)
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Drug Delivery: " Soft-shelled turtle iridovirus (STIV) causes severe systemic disease in farmed soft-shelled turtles (Trionyx sinensis). We generated DNA aptamers that bound STIV with a high level of specificity, providing an alternative means for investigating STIV pathogenesis, drug development, and medical therapies for STIV infection. These DNA aptamers may thus be suitable antiviral candidates for the control of STIV infections."
Reference (2015, BMC Vet Res ): Li, P., Zhou, L., Yu, Y., Yang, M., Ni, S., Wei, S., & Qin, Q. (2015). Characterization of DNA aptamers generated against the soft-shelled turtle iridovirus with antiviral effects. BMC Veterinary Research, 11.  https://doi.org/10.1186/s12917-015-0559-6
Aptamer: QA-12
Target: Soft-shelled turtle iridovirus (STIV)
Type of nucleic acid: ssDNA
Sequence: 5'GACGCTTACTCAGGTGTGACTCGAGTGGGTAAGGTCTGGTGGATTGTGGACGGGGGGCGGGGCAGTGGCTTGACGAAGGACGCAGAGAAGTCTC3'
Sequence length: 94
GC content: 0.6063829787234043
Affinity: Kd: 80.7 nM
Pool type: 5'-GACGCTTACTCAGGTGTGACTCG-N50-CGAAGGACGCAGAGAAGTCTC-3'
Pool random region: 50
Binding buffer/conditions: 100 mM NaCl, 2.5 mM MgCl2, 20 mM Tris–HCl (pH 7.5)
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Drug Delivery: " Soft-shelled turtle iridovirus (STIV) causes severe systemic disease in farmed soft-shelled turtles (Trionyx sinensis). We generated DNA aptamers that bound STIV with a high level of specificity, providing an alternative means for investigating STIV pathogenesis, drug development, and medical therapies for STIV infection. These DNA aptamers may thus be suitable antiviral candidates for the control of STIV infections."
Reference (2015, BMC Vet Res ): Li, P., Zhou, L., Yu, Y., Yang, M., Ni, S., Wei, S., & Qin, Q. (2015). Characterization of DNA aptamers generated against the soft-shelled turtle iridovirus with antiviral effects. BMC Veterinary Research, 11.  https://doi.org/10.1186/s12917-015-0559-6
Aptamer: QA-36
Target: Soft-shelled turtle iridovirus (STIV)
Type of nucleic acid: ssDNA
Sequence: 5'GACGCTTACTCAGGTGTGACTCGTGTGCGGGGGAGGGGAGTGGCGCTGTTGGTGCGGGTATAGCGCGTGGTGTCGAAGGACGCAGAGAAGTCTC3'
Sequence length: 94
GC content: 0.6276595744680851
Affinity: Kd: 53.8 nM
Pool type: 5'-GACGCTTACTCAGGTGTGACTCG-N50-CGAAGGACGCAGAGAAGTCTC-3'
Pool random region: 50
Binding buffer/conditions: 100 mM NaCl, 2.5 mM MgCl2, 20 mM Tris–HCl (pH 7.5)
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Drug Delivery: " Soft-shelled turtle iridovirus (STIV) causes severe systemic disease in farmed soft-shelled turtles (Trionyx sinensis). We generated DNA aptamers that bound STIV with a high level of specificity, providing an alternative means for investigating STIV pathogenesis, drug development, and medical therapies for STIV infection. These DNA aptamers may thus be suitable antiviral candidates for the control of STIV infections."
Reference (2015, BMC Vet Res ): Li, P., Zhou, L., Yu, Y., Yang, M., Ni, S., Wei, S., & Qin, Q. (2015). Characterization of DNA aptamers generated against the soft-shelled turtle iridovirus with antiviral effects. BMC Veterinary Research, 11.  https://doi.org/10.1186/s12917-015-0559-6
Aptamer: QA-17
Target: Soft-shelled turtle iridovirus (STIV)
Type of nucleic acid: ssDNA
Sequence: 5'GACGCTTACTCAGGTGTGACTCGAGAGGGTCGGTCGTAGTGGATTTGGCGCATTGTTCTGCGGGGTGGGAGGGCGAAGGACGCAGAGAAGTCTC3'
Sequence length: 94
GC content: 0.5957446808510638
Affinity: Not reported
Pool type: 5'-GACGCTTACTCAGGTGTGACTCG-N50-CGAAGGACGCAGAGAAGTCTC-3'
Pool random region: 50
Binding buffer/conditions: 100 mM NaCl, 2.5 mM MgCl2, 20 mM Tris–HCl (pH 7.5)
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Drug Delivery: " Soft-shelled turtle iridovirus (STIV) causes severe systemic disease in farmed soft-shelled turtles (Trionyx sinensis). We generated DNA aptamers that bound STIV with a high level of specificity, providing an alternative means for investigating STIV pathogenesis, drug development, and medical therapies for STIV infection. These DNA aptamers may thus be suitable antiviral candidates for the control of STIV infections."
Reference (2015, BMC Vet Res ): Li, P., Zhou, L., Yu, Y., Yang, M., Ni, S., Wei, S., & Qin, Q. (2015). Characterization of DNA aptamers generated against the soft-shelled turtle iridovirus with antiviral effects. BMC Veterinary Research, 11.  https://doi.org/10.1186/s12917-015-0559-6
Aptamer: QA-79
Target: Soft-shelled turtle iridovirus (STIV)
Type of nucleic acid: ssDNA
Sequence: 5'GACGCTTACTCAGGTGTGACTCGGTCGGGACAGTGTTGGTCCTCAGGATCTCTGGGGCGCGGGGTTAAACAGTCGAAGGACGCAGAGAAGTCTC3'
Sequence length: 94
GC content: 0.5851063829787234
Affinity: Not reported
Pool type: 5'-GACGCTTACTCAGGTGTGACTCG-N50-CGAAGGACGCAGAGAAGTCTC-3'
Pool random region: 50
Binding buffer/conditions: 100 mM NaCl, 2.5 mM MgCl2, 20 mM Tris–HCl (pH 7.5)
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Drug Delivery: " Soft-shelled turtle iridovirus (STIV) causes severe systemic disease in farmed soft-shelled turtles (Trionyx sinensis). We generated DNA aptamers that bound STIV with a high level of specificity, providing an alternative means for investigating STIV pathogenesis, drug development, and medical therapies for STIV infection. These DNA aptamers may thus be suitable antiviral candidates for the control of STIV infections."
Reference (2015, BMC Vet Res ): Li, P., Zhou, L., Yu, Y., Yang, M., Ni, S., Wei, S., & Qin, Q. (2015). Characterization of DNA aptamers generated against the soft-shelled turtle iridovirus with antiviral effects. BMC Veterinary Research, 11.  https://doi.org/10.1186/s12917-015-0559-6
Aptamer: QA-88
Target: Soft-shelled turtle iridovirus (STIV)
Type of nucleic acid: ssDNA
Sequence: 5'GACGCTTACTCAGGTGTGACTCGGGATAGGGGGCTCGCTCGTTCCGTAGCGATGCAGGTTTCTTATTCACGAACGAAGGACGCAGAGAAGTCTC3'
Sequence length: 94
GC content: 0.5531914893617021
Affinity: Not reported
Pool type: 5'-GACGCTTACTCAGGTGTGACTCG-N50-CGAAGGACGCAGAGAAGTCTC-3'
Pool random region: 50
Binding buffer/conditions: 100 mM NaCl, 2.5 mM MgCl2, 20 mM Tris–HCl (pH 7.5)
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Drug Delivery: " Soft-shelled turtle iridovirus (STIV) causes severe systemic disease in farmed soft-shelled turtles (Trionyx sinensis). We generated DNA aptamers that bound STIV with a high level of specificity, providing an alternative means for investigating STIV pathogenesis, drug development, and medical therapies for STIV infection. These DNA aptamers may thus be suitable antiviral candidates for the control of STIV infections."
Reference (2015, BMC Vet Res ): Li, P., Zhou, L., Yu, Y., Yang, M., Ni, S., Wei, S., & Qin, Q. (2015). Characterization of DNA aptamers generated against the soft-shelled turtle iridovirus with antiviral effects. BMC Veterinary Research, 11.  https://doi.org/10.1186/s12917-015-0559-6
Aptamer: C-ap17P
Target: N-terminal region of Recombinant human connective tissue growth factor (rhCTGF)
Type of nucleic acid: ssDNA
Sequence: 5'CTGTATGCCTCCTGTACGAGCGGTGATTCTTGTCCAGCCCTGTATGCCTCCTGTACGAGCGGTGATTCTTGTCCA3'
Sequence length: 75
GC content: 0.5466666666666666
Affinity: Kd: 3.648 nM
Pool type: 5′-GACAAGAATCACCGCTC-N40-CGTACAGGAGGCATACAG-3′
Pool random region: 40
Binding buffer/conditions: 10 μg yeast tRNA and 100 μg BSA in 100 μL SHMCK buffer (20 mM Hepes, 120 mM NaCl, 5 mM KCl, 1 mM MgCl2 and 1 mM CaCl2, pH 7.4)
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Connective tissue growth factor (CTGF) is a secreted matricellular protein possessing complex biological functions. In this study, we selected CTGF-targeting DNA aptamers by using systematic evolution of ligands by exponential enrichment (SELEX). These CTGF aptamers might be used as a easy and fast detection tool for CTGF and be developed as CTGF-specific inhibitors for both research works and clinical applications."
Additional information: The highest affinity aptamer against rhCTGF developed by Li et al.; Included the constant regions. Biotin modified at the 5' end
Reference (2015, Biochem Biophys Res Commun ): Li, S., Huo, Y., Tian, H., Zhang, Q., Lv, Y., & Hao, Z. (2015). In vitro selection and characterization of deoxyribonucleic acid aptamers against connective tissue growth factor. Biochemical and biophysical research communications, 457(4), 640–646. https://doi.org/10.1016/j.bbrc.2015.01.041
Aptamer: C-ap11
Target: C-terminal region Recombinant human connective tissue growth factor (rhCTGF)
Type of nucleic acid: ssDNA
Sequence: 5′GGACAAGAATCACCGCTCCCCGTACAGGAGGCATACAGA3′
Sequence length: 39
GC content: 0.5641025641025641
Affinity: Kd: 7.376 nM
Pool type: 5′-GACAAGAATCACCGCTC-N40-CGTACAGGAGGCATACAG-3′
Pool random region: 40
Binding buffer/conditions: 10 μg yeast tRNA and 100 μg BSA in 100 μL SHMCK buffer (20 mM Hepes, 120 mM NaCl, 5 mM KCl, 1 mM MgCl2 and 1 mM CaCl2, pH 7.4)
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Connective tissue growth factor (CTGF) is a secreted matricellular protein possessing complex biological functions. In this study, we selected CTGF-targeting DNA aptamers by using systematic evolution of ligands by exponential enrichment (SELEX). These CTGF aptamers might be used as a easy and fast detection tool for CTGF and be developed as CTGF-specific inhibitors for both research works and clinical applications."
Additional information: The synthetic aptamer characterized as c-ap11 only consisted of the core, random region due to mutations and deletions during amplification. Biotin was used to label the 3' end of this aptamer
Reference (2015, Biochem Biophys Res Commun ): Li, S., Huo, Y., Tian, H., Zhang, Q., Lv, Y., & Hao, Z. (2015). In vitro selection and characterization of deoxyribonucleic acid aptamers against connective tissue growth factor. Biochemical and biophysical research communications, 457(4), 640–646. https://doi.org/10.1016/j.bbrc.2015.01.041
Aptamer: C-ap12
Target: C-terminal region Recombinant human connective tissue growth factor (rhCTGF)
Type of nucleic acid: ssDNA
Sequence: 5′GGACAAGAATCACCGCTCGTACAGGAGGCATACAGA3′
Sequence length: 36
GC content: 0.5277777777777778
Affinity: Kd: 3.893 nM
Pool type: 5′-GACAAGAATCACCGCTC-N40-CGTACAGGAGGCATACAG-3′
Pool random region: 40
Binding buffer/conditions: 10 μg yeast tRNA and 100 μg BSA in 100 μL SHMCK buffer (20 mM Hepes, 120 mM NaCl, 5 mM KCl, 1 mM MgCl2 and 1 mM CaCl2, pH 7.4)
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Connective tissue growth factor (CTGF) is a secreted matricellular protein possessing complex biological functions. In this study, we selected CTGF-targeting DNA aptamers by using systematic evolution of ligands by exponential enrichment (SELEX). These CTGF aptamers might be used as a easy and fast detection tool for CTGF and be developed as CTGF-specific inhibitors for both research works and clinical applications."
Additional information: The synthetic aptamer characterized as c-ap12 only consisted of the core, random region due to mutations and deletions during amplification. Biotin was used to label the 5' end of this aptamer
Reference (2015, Biochem Biophys Res Commun ): Li, S., Huo, Y., Tian, H., Zhang, Q., Lv, Y., & Hao, Z. (2015). In vitro selection and characterization of deoxyribonucleic acid aptamers against connective tissue growth factor. Biochemical and biophysical research communications, 457(4), 640–646. https://doi.org/10.1016/j.bbrc.2015.01.041
Aptamer: C-ap14
Target: C-terminal region Recombinant human connective tissue growth factor (rhCTGF)
Type of nucleic acid: ssDNA
Sequence: 5′CTGTATGCCTCCTGTACGGGGAGCGGTGATTCTTGTCCA3′
Sequence length: 39
GC content: 0.5641025641025641
Affinity: Kd: 5.561 nM
Pool type: 5′-GACAAGAATCACCGCTC-N40-CGTACAGGAGGCATACAG-3′
Pool random region: 40
Binding buffer/conditions: 10 μg yeast tRNA and 100 μg BSA in 100 μL SHMCK buffer (20 mM Hepes, 120 mM NaCl, 5 mM KCl, 1 mM MgCl2 and 1 mM CaCl2, pH 7.4)
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Connective tissue growth factor (CTGF) is a secreted matricellular protein possessing complex biological functions. In this study, we selected CTGF-targeting DNA aptamers by using systematic evolution of ligands by exponential enrichment (SELEX). These CTGF aptamers might be used as a easy and fast detection tool for CTGF and be developed as CTGF-specific inhibitors for both research works and clinical applications."
Additional information: The synthetic aptamer characterized as c-ap14 only consisted of the core, random region due to mutations and deletions during amplification. Biotin was used to label the 5' end of this aptamer
Reference (2015, Biochem Biophys Res Commun ): Li, S., Huo, Y., Tian, H., Zhang, Q., Lv, Y., & Hao, Z. (2015). In vitro selection and characterization of deoxyribonucleic acid aptamers against connective tissue growth factor. Biochemical and biophysical research communications, 457(4), 640–646. https://doi.org/10.1016/j.bbrc.2015.01.041