RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: E7
Target: Escherichia coli (E. coli) core bacterial RNA polymerase (RNAP)
Type of nucleic acid: ssDNA
Sequence: 5'GGGAGCTCAGAATAAACGCTCAATGTGAGCTGGTGGGGTAGGGGGCTGCGAGAGTTTCGACATGAGGCCCGGATC3'
Sequence length: 75
GC content: 0.5866666666666667
Affinity: Kd: 2.21 nM
Pool type: 5'-GGGAGCTCAGAATAAACGCTCAA-N32-TTCGACATGAGGCCCGGATC-3'
Pool random region: 32
Binding buffer/conditions: 20 mM Tris/HCl pH 7.9, 10 mM MgCl2, 300 mM NaCl, 30 mM KCl; in subsequent rounds NaCl and KCl concentrations were increased to 400 and 40 mM, respectively
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.9
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Bacterial RNA polymerase (RNAP) is the central enzyme of gene expression that is responsible for the synthesis of all types of cellular RNAs. The goal of this work was to obtain novel ligands to RNAP which would target different epitopes of the enzyme and serve as specific probes to study the mechanism of transcription and conformational flexibility of RNAP. We propose that the aptamers obtained in this work will be useful for studying the interactions of RNAP with various ligands and regulatory factors and for investigating the conformational flexibility of the enzyme."
Reference (2004, Eur J Biochem ): Kulbachinskiy, A., Feklistov, A., Krasheninnikov, I., Goldfarb, A., & Nikiforov, V. (2004). Aptamers to Escherichia coli core RNA polymerase that sense its interaction with rifampicin, sigma-subunit and GreB. European journal of biochemistry, 271(23-24), 4921–4931. https://doi.org/10.1111/j.1432-1033.2004.04461.x
Aptamer: E8
Target: Escherichia coli (E. coli) core bacterial RNA polymerase (RNAP)
Type of nucleic acid: ssDNA
Sequence: 5'GGGAGCTCAGAATAAACGCTCAACTGCCCACCGCTGTAAGGTGTCGCCTCCGTCATTCGACATGAGGCCCGGATC3'
Sequence length: 75
GC content: 0.5866666666666667
Affinity: Kd: 6.32 nM
Pool type: 5'-GGGAGCTCAGAATAAACGCTCAA-N32-TTCGACATGAGGCCCGGATC-3'
Pool random region: 32
Binding buffer/conditions: 20 mM Tris/HCl pH 7.9, 10 mM MgCl2, 300 mM NaCl, 30 mM KCl; in subsequent rounds NaCl and KCl concentrations were increased to 400 and 40 mM, respectively
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.9
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Bacterial RNA polymerase (RNAP) is the central enzyme of gene expression that is responsible for the synthesis of all types of cellular RNAs. The goal of this work was to obtain novel ligands to RNAP which would target different epitopes of the enzyme and serve as specific probes to study the mechanism of transcription and conformational flexibility of RNAP. We propose that the aptamers obtained in this work will be useful for studying the interactions of RNAP with various ligands and regulatory factors and for investigating the conformational flexibility of the enzyme."
Reference (2004, Eur J Biochem ): Kulbachinskiy, A., Feklistov, A., Krasheninnikov, I., Goldfarb, A., & Nikiforov, V. (2004). Aptamers to Escherichia coli core RNA polymerase that sense its interaction with rifampicin, sigma-subunit and GreB. European journal of biochemistry, 271(23-24), 4921–4931. https://doi.org/10.1111/j.1432-1033.2004.04461.x
Aptamer: E9
Target: Escherichia coli (E. coli) core bacterial RNA polymerase (RNAP)
Type of nucleic acid: ssDNA
Sequence: 5'GGGAGCTCAGAATAAACGCTCAACTCGCTACAGCAAACGCAGCCTCGAGGGATGGTTCGACATGAGGCCCGGATC3'
Sequence length: 75
GC content: 0.5733333333333334
Affinity: Kd: 1.43 nM
Pool type: 5'-GGGAGCTCAGAATAAACGCTCAA-N32-TTCGACATGAGGCCCGGATC-3'
Pool random region: 32
Binding buffer/conditions: 20 mM Tris/HCl pH 7.9, 10 mM MgCl2, 300 mM NaCl, 30 mM KCl; in subsequent rounds NaCl and KCl concentrations were increased to 400 and 40 mM, respectively
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.9
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Bacterial RNA polymerase (RNAP) is the central enzyme of gene expression that is responsible for the synthesis of all types of cellular RNAs. The goal of this work was to obtain novel ligands to RNAP which would target different epitopes of the enzyme and serve as specific probes to study the mechanism of transcription and conformational flexibility of RNAP. We propose that the aptamers obtained in this work will be useful for studying the interactions of RNAP with various ligands and regulatory factors and for investigating the conformational flexibility of the enzyme."
Reference (2004, Eur J Biochem ): Kulbachinskiy, A., Feklistov, A., Krasheninnikov, I., Goldfarb, A., & Nikiforov, V. (2004). Aptamers to Escherichia coli core RNA polymerase that sense its interaction with rifampicin, sigma-subunit and GreB. European journal of biochemistry, 271(23-24), 4921–4931. https://doi.org/10.1111/j.1432-1033.2004.04461.x
Aptamer: E10
Target: Escherichia coli (E. coli) core bacterial RNA polymerase (RNAP)
Type of nucleic acid: ssDNA
Sequence: 5'GGGAGCTCAGAATAAACGCTCAAGGCATCCACGCACTGCGAACCGGTCTGGAGTGTTCGACATGAGGCCCGGATC3'
Sequence length: 75
GC content: 0.5866666666666667
Affinity: Kd: 2.82 nM
Pool type: 5'-GGGAGCTCAGAATAAACGCTCAA-N32-TTCGACATGAGGCCCGGATC-3'
Pool random region: 32
Binding buffer/conditions: 20 mM Tris/HCl pH 7.9, 10 mM MgCl2, 300 mM NaCl, 30 mM KCl; in subsequent rounds NaCl and KCl concentrations were increased to 400 and 40 mM, respectively
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.9
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Bacterial RNA polymerase (RNAP) is the central enzyme of gene expression that is responsible for the synthesis of all types of cellular RNAs. The goal of this work was to obtain novel ligands to RNAP which would target different epitopes of the enzyme and serve as specific probes to study the mechanism of transcription and conformational flexibility of RNAP. We propose that the aptamers obtained in this work will be useful for studying the interactions of RNAP with various ligands and regulatory factors and for investigating the conformational flexibility of the enzyme."
Reference (2004, Eur J Biochem ): Kulbachinskiy, A., Feklistov, A., Krasheninnikov, I., Goldfarb, A., & Nikiforov, V. (2004). Aptamers to Escherichia coli core RNA polymerase that sense its interaction with rifampicin, sigma-subunit and GreB. European journal of biochemistry, 271(23-24), 4921–4931. https://doi.org/10.1111/j.1432-1033.2004.04461.x
Aptamer: E11
Target: Escherichia coli (E. coli) core bacterial RNA polymerase (RNAP)
Type of nucleic acid: ssDNA
Sequence: 5'GGGAGCTCAGAATAAACGCTCAAGGAGTGATGCCGCCGCCTGGTTGCCTTGGGAGTTCGACATGAGGCCCGGATC3'
Sequence length: 75
GC content: 0.6
Affinity: Kd: 3.56 nM
Pool type: 5'-GGGAGCTCAGAATAAACGCTCAA-N32-TTCGACATGAGGCCCGGATC-3'
Pool random region: 32
Binding buffer/conditions: 20 mM Tris/HCl pH 7.9, 10 mM MgCl2, 300 mM NaCl, 30 mM KCl; in subsequent rounds NaCl and KCl concentrations were increased to 400 and 40 mM, respectively
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.9
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Bacterial RNA polymerase (RNAP) is the central enzyme of gene expression that is responsible for the synthesis of all types of cellular RNAs. The goal of this work was to obtain novel ligands to RNAP which would target different epitopes of the enzyme and serve as specific probes to study the mechanism of transcription and conformational flexibility of RNAP. We propose that the aptamers obtained in this work will be useful for studying the interactions of RNAP with various ligands and regulatory factors and for investigating the conformational flexibility of the enzyme."
Reference (2004, Eur J Biochem ): Kulbachinskiy, A., Feklistov, A., Krasheninnikov, I., Goldfarb, A., & Nikiforov, V. (2004). Aptamers to Escherichia coli core RNA polymerase that sense its interaction with rifampicin, sigma-subunit and GreB. European journal of biochemistry, 271(23-24), 4921–4931. https://doi.org/10.1111/j.1432-1033.2004.04461.x
Aptamer: E12
Target: Escherichia coli (E. coli) core bacterial RNA polymerase (RNAP)
Type of nucleic acid: ssDNA
Sequence: 5'GGGAGCTCAGAATAAACGCTCAAGCATCCAGCAAACGCAGCCGGTTTGGCGCCTATTCGACATGAGGCCCGGATC3'
Sequence length: 75
GC content: 0.5733333333333334
Affinity: Kd: 4.00 nM
Pool type: 5'-GGGAGCTCAGAATAAACGCTCAA-N32-TTCGACATGAGGCCCGGATC-3'
Pool random region: 32
Binding buffer/conditions: 20 mM Tris/HCl pH 7.9, 10 mM MgCl2, 300 mM NaCl, 30 mM KCl; in subsequent rounds NaCl and KCl concentrations were increased to 400 and 40 mM, respectively
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.9
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Bacterial RNA polymerase (RNAP) is the central enzyme of gene expression that is responsible for the synthesis of all types of cellular RNAs. The goal of this work was to obtain novel ligands to RNAP which would target different epitopes of the enzyme and serve as specific probes to study the mechanism of transcription and conformational flexibility of RNAP. We propose that the aptamers obtained in this work will be useful for studying the interactions of RNAP with various ligands and regulatory factors and for investigating the conformational flexibility of the enzyme."
Reference (2004, Eur J Biochem ): Kulbachinskiy, A., Feklistov, A., Krasheninnikov, I., Goldfarb, A., & Nikiforov, V. (2004). Aptamers to Escherichia coli core RNA polymerase that sense its interaction with rifampicin, sigma-subunit and GreB. European journal of biochemistry, 271(23-24), 4921–4931. https://doi.org/10.1111/j.1432-1033.2004.04461.x
Aptamer: E13
Target: Escherichia coli (E. coli) core bacterial RNA polymerase (RNAP)
Type of nucleic acid: ssDNA
Sequence: 5'GGGAGCTCAGAATAAACGCTCAAGCTACTGCTGGAGCACACCCCGACGGTGAGAGTCGACATGAGGCCCGGATC3'
Sequence length: 74
GC content: 0.5945945945945946
Affinity: Kd: 4.93 nM
Pool type: 5'-GGGAGCTCAGAATAAACGCTCAA-N32-TTCGACATGAGGCCCGGATC-3'
Pool random region: 32
Binding buffer/conditions: 20 mM Tris/HCl pH 7.9, 10 mM MgCl2, 300 mM NaCl, 30 mM KCl; in subsequent rounds NaCl and KCl concentrations were increased to 400 and 40 mM, respectively
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.9
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Bacterial RNA polymerase (RNAP) is the central enzyme of gene expression that is responsible for the synthesis of all types of cellular RNAs. The goal of this work was to obtain novel ligands to RNAP which would target different epitopes of the enzyme and serve as specific probes to study the mechanism of transcription and conformational flexibility of RNAP. We propose that the aptamers obtained in this work will be useful for studying the interactions of RNAP with various ligands and regulatory factors and for investigating the conformational flexibility of the enzyme."
Additional information: Deletion of T at the first position of the right constant region
Reference (2004, Eur J Biochem ): Kulbachinskiy, A., Feklistov, A., Krasheninnikov, I., Goldfarb, A., & Nikiforov, V. (2004). Aptamers to Escherichia coli core RNA polymerase that sense its interaction with rifampicin, sigma-subunit and GreB. European journal of biochemistry, 271(23-24), 4921–4931. https://doi.org/10.1111/j.1432-1033.2004.04461.x
Aptamer: apt1
Target: Receptor activator of NF-kappaB (RANK), Human
Type of nucleic acid: ssRNA
Sequence: 5′ACACAAUGGACGGAUUCGUAUGGGUGGGAUCGGGAAGGGCUACGAACACCGUAACGGCCGACAUGAGAG3′
Sequence length: 69
GC content: 0.5652173913043478
Affinity: Kd: 0.33 uM
Pool type: 5′-GGACACAATGGACG-N40-TAACGGCCGACATGAGAG-3′
Pool random region: 40
Binding buffer/conditions: 20 mM Tris–HCl, pH 7.6, 80 mM potassium acetate, 2.5 mM magnesium acetate and 1 mM DTT
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " Generating RNA aptamers against human RANK to develop potential therapeutics for RA and bone malignancies. In the literature, only a few RNA aptamers are known to be reactive to membrane proteins including receptors. Of these, RNA aptamers against hepatocyte growth factor (HGF) receptor (c-met) were successfully generated by SELEX when HGF's soluble extracellular domain was used as the target molecule"
Reference (2004, Nucleic Acids Res ): Mori, T., Oguro, A., Ohtsu, T., & Nakamura, Y. (2004). RNA aptamers selected against the receptor activator of NF-kappaB acquire general affinity to proteins of the tumor necrosis factor receptor family. Nucleic acids research, 32(20), 6120–6128. https://doi.org/10.1093/nar/gkh949
Aptamer: apt2
Target: Receptor activator of NF-kappaB (RANK), Human
Type of nucleic acid: ssRNA
Sequence: 5′ACACAAUGGACGAUAUGGUAUUGAGGUGGGAAGGGGAUUGGGAUAAUUGCCAUAACGGCCGACAUGAGAG3′
Sequence length: 70
GC content: 0.4857142857142857
Affinity: Kd: 1.8 uM
Pool type: 5′-GGACACAATGGACG-N40-TAACGGCCGACATGAGAG-3′
Pool random region: 40
Binding buffer/conditions: 20 mM Tris–HCl, pH 7.6, 80 mM potassium acetate, 2.5 mM magnesium acetate and 1 mM DTT
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " Generating RNA aptamers against human RANK to develop potential therapeutics for RA and bone malignancies. In the literature, only a few RNA aptamers are known to be reactive to membrane proteins including receptors. Of these, RNA aptamers against hepatocyte growth factor (HGF) receptor (c-met) were successfully generated by SELEX when HGF's soluble extracellular domain was used as the target molecule"
Reference (2004, Nucleic Acids Res ): Mori, T., Oguro, A., Ohtsu, T., & Nakamura, Y. (2004). RNA aptamers selected against the receptor activator of NF-kappaB acquire general affinity to proteins of the tumor necrosis factor receptor family. Nucleic acids research, 32(20), 6120–6128. https://doi.org/10.1093/nar/gkh949
Aptamer: apt3
Target: Receptor activator of NF-kappaB (RANK), Human
Type of nucleic acid: ssRNA
Sequence: 5′ACACAAUGGACGAUAGUGGUUGGGAUUGGGUGGGCGCACAAGUUGUUCCGUUAACGGCCGACAUGAGAG3′
Sequence length: 69
GC content: 0.5362318840579711
Affinity: Kd: 5.8 uM
Pool type: 5′-GGACACAATGGACG-N40-TAACGGCCGACATGAGAG-3′
Pool random region: 40
Binding buffer/conditions: 20 mM Tris–HCl, pH 7.6, 80 mM potassium acetate, 2.5 mM magnesium acetate and 1 mM DTT
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " Generating RNA aptamers against human RANK to develop potential therapeutics for RA and bone malignancies. In the literature, only a few RNA aptamers are known to be reactive to membrane proteins including receptors. Of these, RNA aptamers against hepatocyte growth factor (HGF) receptor (c-met) were successfully generated by SELEX when HGF's soluble extracellular domain was used as the target molecule"
Reference (2004, Nucleic Acids Res ): Mori, T., Oguro, A., Ohtsu, T., & Nakamura, Y. (2004). RNA aptamers selected against the receptor activator of NF-kappaB acquire general affinity to proteins of the tumor necrosis factor receptor family. Nucleic acids research, 32(20), 6120–6128. https://doi.org/10.1093/nar/gkh949
Aptamer: 3.1
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUACCCCGGGUUCUGUAUGAUCCGACCGGUCAGAUAAGACCACAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.5352112676056338
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 7.5
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUACGCAUCGUUUGCGUGGCGUGUCCGGGCGCCGAUUCGUAAAAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.5633802816901409
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 14.8
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUACGUAUAUACGGAUUAGGUUGUAGCUCAGACCAGUAAUGUCAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.4507042253521127
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 16.3
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUACGUGCUAGAUGCUACGAGUGGUCUCCUCACGUAGAAGGGGAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.5352112676056338
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 18.1
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUAGGUCCCACAUAGGUUGGUCUUGUUGUAUGGGCUGUUUGCAAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.4929577464788732
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 1
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUAGUGUUUUGGGAGAGAAAAGGGGGAGCCUUUACUUUGUUGGAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.4788732394366197
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4