RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: C18
Target: Human immunodeficiency virus type 1 Rev (HIV-1 Rev)
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAUACCAGCUUAUUCAAUUGCUAGGCAAUGUUUCGGUUGGAGUAAUCCGGUGGCUUGCCAUGAUUUACGUGAGUGCUGAUCCGUGAUGAGAUAGUAAGUGCAAUCU3'
Sequence length: 110
GC content: 0.4545454545454545
Affinity: Kd: 19-36 nM
Pool type: 5'-GGGAGAUACCAGCUUAUUCAAUU-N71-AGAUAGUAAGUGCAAUCU-3'
Pool random region: 71
Binding buffer/conditions: 500 ul of 10 mM Hepes, pH 7.4/100 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "RNA aptamers were selected to bind to the isolated Rev34_50 peptide. These results suggest that artificially selected binding cusps may have much in common with those found on natural molecules and have important implications for the development of novel diagnostic reagents and strategies for intracellular immunization"
Additional information: Pool was not stated, but I figured it out using the figures
Reference (1996, Proc Natl Acad Sci U S A ): Xu, W., & Ellington, A. D. (1996). Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope. Proceedings of the National Academy of Sciences of the United States of America, 93(15), 7475–7480. https://doi.org/10.1073/pnas.93.15.7475
Aptamer: C17
Target: Human immunodeficiency virus type 1 Rev (HIV-1 Rev)
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAUACCAGCUUAUUCAAUUGUAUUCUCGGUGGUUUAAUCUGUGUAGAGGAGCUGACUCCUUUGGUUGGACUACGUGGAGGUUCUCUUAGAUAGUAAGUGCAAUCU3'
Sequence length: 109
GC content: 0.4311926605504587
Affinity: Kd: 19-36 nM
Pool type: 5'-GGGAGAUACCAGCUUAUUCAAUU-N71-AGAUAGUAAGUGCAAUCU-3'
Pool random region: 71
Binding buffer/conditions: 500 ul of 10 mM Hepes, pH 7.4/100 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "RNA aptamers were selected to bind to the isolated Rev34_50 peptide. These results suggest that artificially selected binding cusps may have much in common with those found on natural molecules and have important implications for the development of novel diagnostic reagents and strategies for intracellular immunization"
Additional information: Pool was not stated, but I figured it out using the figures
Reference (1996, Proc Natl Acad Sci U S A ): Xu, W., & Ellington, A. D. (1996). Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope. Proceedings of the National Academy of Sciences of the United States of America, 93(15), 7475–7480. https://doi.org/10.1073/pnas.93.15.7475
Aptamer: C1
Target: Human immunodeficiency virus type 1 Rev (HIV-1 Rev)
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAUACCAGCUUAUUCAAUUGCAGUUAACCAAGCCUGCAUACUGGAUAGACGGCUUAUCCGACUGAAUGCCUCCCGAAAGGUGCAGUUAGAUAGUAAGUGCAAUCU3'
Sequence length: 109
GC content: 0.4587155963302752
Affinity: Kd: 19-36 nM
Pool type: 5'-GGGAGAUACCAGCUUAUUCAAUU-N71-AGAUAGUAAGUGCAAUCU-3'
Pool random region: 71
Binding buffer/conditions: 500 ul of 10 mM Hepes, pH 7.4/100 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "RNA aptamers were selected to bind to the isolated Rev34_50 peptide. These results suggest that artificially selected binding cusps may have much in common with those found on natural molecules and have important implications for the development of novel diagnostic reagents and strategies for intracellular immunization"
Additional information: Pool was not stated, but I figured it out using the figures
Reference (1996, Proc Natl Acad Sci U S A ): Xu, W., & Ellington, A. D. (1996). Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope. Proceedings of the National Academy of Sciences of the United States of America, 93(15), 7475–7480. https://doi.org/10.1073/pnas.93.15.7475
Aptamer: C9
Target: Human immunodeficiency virus type 1 Rev (HIV-1 Rev)
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAUACCAGCUUAUUCAAUUGCGCAAACCCGAAGAAUGCCCAAAUUGAUCCAGAGCAAGUGGGAAUGAUAUAAAGUACCUGGUCCUGGAGAUAGUAAGUGCAAUCU3'
Sequence length: 109
GC content: 0.4403669724770642
Affinity: Kd: 19-36 nM
Pool type: 5'-GGGAGAUACCAGCUUAUUCAAUU-N71-AGAUAGUAAGUGCAAUCU-3'
Pool random region: 71
Binding buffer/conditions: 500 ul of 10 mM Hepes, pH 7.4/100 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic  and Therapeutic: "RNA aptamers were selected to bind to the isolated Rev34_50 peptide. These results suggest that artificially selected binding cusps may have much in common with those found on natural molecules and have important implications for the development of novel diagnostic reagents and strategies for intracellular immunization"
Additional information: Pool was not stated, but I figured it out using the figures
Reference (1996, Proc Natl Acad Sci U S A ): Xu, W., & Ellington, A. D. (1996). Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope. Proceedings of the National Academy of Sciences of the United States of America, 93(15), 7475–7480. https://doi.org/10.1073/pnas.93.15.7475
Aptamer: C15
Target: Human immunodeficiency virus type 1 Rev (HIV-1 Rev)
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAUACCAGCUUAUUCAAUUUCCAAACCCCGUUGAGAGUUGAUCCGGUCUAGGGAAUGGGAAAGAAGUAGGUAUCGAAGAGAAUGUACCCUAGAUAGUAAGUGCAAUCU3'
Sequence length: 112
GC content: 0.4375
Affinity: Kd: 19-36 nM
Pool type: 5'-GGGAGAUACCAGCUUAUUCAAUU-N71-AGAUAGUAAGUGCAAUCU-3'
Pool random region: 71
Binding buffer/conditions: 500 ul of 10 mM Hepes, pH 7.4/100 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "RNA aptamers were selected to bind to the isolated Rev34_50 peptide. These results suggest that artificially selected binding cusps may have much in common with those found on natural molecules and have important implications for the development of novel diagnostic reagents and strategies for intracellular immunization"
Additional information: Pool was not stated, but I figured it out using the figures
Reference (1996, Proc Natl Acad Sci U S A ): Xu, W., & Ellington, A. D. (1996). Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope. Proceedings of the National Academy of Sciences of the United States of America, 93(15), 7475–7480. https://doi.org/10.1073/pnas.93.15.7475
Aptamer: C24
Target: Human immunodeficiency virus type 1 Rev (HIV-1 Rev)
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAUACCAGCUUAUUCAAUUAGGACUCAAAUAUUCACGUUGACGUUGUCUUGGAGUGCUGAUCGGAAAACCAAUAUGAUUAAUGGGUCCUGAGAUAGUAAGUGCAAUCU3'
Sequence length: 112
GC content: 0.4017857142857143
Affinity: Kd: 19-36 nM
Pool type: 5'-GGGAGAUACCAGCUUAUUCAAUU-N71-AGAUAGUAAGUGCAAUCU-3'
Pool random region: 71
Binding buffer/conditions: 500 ul of 10 mM Hepes, pH 7.4/100 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "RNA aptamers were selected to bind to the isolated Rev34_50 peptide. These results suggest that artificially selected binding cusps may have much in common with those found on natural molecules and have important implications for the development of novel diagnostic reagents and strategies for intracellular immunization"
Additional information: Pool was not stated, but I figured it out using the figures
Reference (1996, Proc Natl Acad Sci U S A ): Xu, W., & Ellington, A. D. (1996). Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope. Proceedings of the National Academy of Sciences of the United States of America, 93(15), 7475–7480. https://doi.org/10.1073/pnas.93.15.7475
Aptamer: C33
Target: Human immunodeficiency virus type 1 Rev (HIV-1 Rev)
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAUACCAGCUUAUUCAAUUACGCAGCGACUGUGGUGGUGAGCGGUUGCGUAACUUGAUUUAAGCAAGUACUGCCAUGGCCGAACCUCUAAGAUAGUAAGUGCAAUCU3'
Sequence length: 111
GC content: 0.4684684684684684
Affinity: Kd: 19-36 nM
Pool type: 5'-GGGAGAUACCAGCUUAUUCAAUU-N71-AGAUAGUAAGUGCAAUCU-3'
Pool random region: 71
Binding buffer/conditions: 500 ul of 10 mM Hepes, pH 7.4/100 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "RNA aptamers were selected to bind to the isolated Rev34_50 peptide. These results suggest that artificially selected binding cusps may have much in common with those found on natural molecules and have important implications for the development of novel diagnostic reagents and strategies for intracellular immunization"
Additional information: Pool was not stated, but I figured it out using the figures
Reference (1996, Proc Natl Acad Sci U S A ): Xu, W., & Ellington, A. D. (1996). Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope. Proceedings of the National Academy of Sciences of the United States of America, 93(15), 7475–7480. https://doi.org/10.1073/pnas.93.15.7475
Aptamer: C34
Target: Human immunodeficiency virus type 1 Rev (HIV-1 Rev)
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAUACCAGCUUAUUCAAUUUCUAGUCAAGUUGCAAUCUCCGGUGGGGUGGUAACCGAGGAACACGUUUCGGGUGUAUAGGCUAGCGAGAUAGUAAGUGCAAUCU3'
Sequence length: 108
GC content: 0.4722222222222222
Affinity: Kd: 19-36 nM
Pool type: 5'-GGGAGAUACCAGCUUAUUCAAUU-N71-AGAUAGUAAGUGCAAUCU-3'
Pool random region: 71
Binding buffer/conditions: 500 ul of 10 mM Hepes, pH 7.4/100 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "RNA aptamers were selected to bind to the isolated Rev34_50 peptide. These results suggest that artificially selected binding cusps may have much in common with those found on natural molecules and have important implications for the development of novel diagnostic reagents and strategies for intracellular immunization"
Additional information: Pool was not stated, but I figured it out using the figures
Reference (1996, Proc Natl Acad Sci U S A ): Xu, W., & Ellington, A. D. (1996). Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope. Proceedings of the National Academy of Sciences of the United States of America, 93(15), 7475–7480. https://doi.org/10.1073/pnas.93.15.7475
Aptamer: C37
Target: Human immunodeficiency virus type 1 Rev (HIV-1 Rev)
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAUACCAGCUUAUUCAAUUUCUACCAGAGCGAGUGUGCUGAACGUUCUAAGGACGGGAUUGAAUCGAGAUGCGUAUACUAGGACCUUACGAGAUAGUAAGUGCAAUCU3'
Sequence length: 112
GC content: 0.4464285714285714
Affinity: Kd: 19-36 nM
Pool type: 5'-GGGAGAUACCAGCUUAUUCAAUU-N71-AGAUAGUAAGUGCAAUCU-3'
Pool random region: 71
Binding buffer/conditions: 500 ul of 10 mM Hepes, pH 7.4/100 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "RNA aptamers were selected to bind to the isolated Rev34_50 peptide. These results suggest that artificially selected binding cusps may have much in common with those found on natural molecules and have important implications for the development of novel diagnostic reagents and strategies for intracellular immunization"
Additional information: Pool was not stated, but I figured it out using the figures
Reference (1996, Proc Natl Acad Sci U S A ): Xu, W., & Ellington, A. D. (1996). Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope. Proceedings of the National Academy of Sciences of the United States of America, 93(15), 7475–7480. https://doi.org/10.1073/pnas.93.15.7475
Aptamer: C52
Target: Human immunodeficiency virus type 1 Rev (HIV-1 Rev)
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAUACCAGCUUAUUCAAUUGCUUGGUACCGAGCUCGGAUCCACGUAGUAACGGGCCGCCAGUGUCUGGAAUUCGGGUCGUUCUUGAGAUAGUAAGUGCAAUCU3'
Sequence length: 107
GC content: 0.5046728971962616
Affinity: Kd: 19-36 nM
Pool type: 5'-GGGAGAUACCAGCUUAUUCAAUU-N71-AGAUAGUAAGUGCAAUCU-3'
Pool random region: 71
Binding buffer/conditions: 500 ul of 10 mM Hepes, pH 7.4/100 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "RNA aptamers were selected to bind to the isolated Rev34_50 peptide. These results suggest that artificially selected binding cusps may have much in common with those found on natural molecules and have important implications for the development of novel diagnostic reagents and strategies for intracellular immunization"
Additional information: Pool was not stated, but I figured it out using the figures
Reference (1996, Proc Natl Acad Sci U S A ): Xu, W., & Ellington, A. D. (1996). Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope. Proceedings of the National Academy of Sciences of the United States of America, 93(15), 7475–7480. https://doi.org/10.1073/pnas.93.15.7475
Aptamer: IGEL1.2
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: 2'-amino-RNA
Sequence: 5'GGGAGGACGAUGCGGGUGUGAAUGGUGUUGUGAGG3'
Sequence length: 35
GC content: 0.6
Affinity: Kd: 30 nM
Pool type: 5'-GGGAGGACGAUGCGG-N40/N60-CAGACGACUCGCCCGA-3'
Pool random region: 60
Binding buffer/conditions: PBS, modified to contain 1 mM of Mgz+ ions (138 mM NaCI, 2.7 mM KCI, 8.1 mM NA,HP04, 1.1 mM KH,P04, and 1 mM MgCI, pH 7.4)
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: 35-nucleotide truncate
Application as quoted in the referenced paper: Therapeutic: " Using the systematic evolution of ligands by exponential enrichment (SELEX) method, we have identified oligonucleotides that bind to human IgE with high affinities and high specificity. Therefore, these oligonucleotide ligands represent a novel class of IgE inhibitors that may prove useful in the fight against allergic diseases."
Additional information: SELEX experiments targeting human IgE were performed using 2'-NH,-modified CTP and UTP
Reference (1996, J Immunol ): Wiegand, T. W., Williams, P. B., Dreskin, S. C., Jouvin, M. H., Kinet, J. P., & Tasset, D. (1996). High-affinity oligonucleotide ligands to human IgE inhibit binding to Fc epsilon receptor I. Journal of immunology (Baltimore, Md. : 1950), 157(1), 221–230.
Aptamer: IGEL2.2
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: 2'-amino-RNA
Sequence: 5'GGGAGGACGAUGCGGGUGUGGGGCG3'
Sequence length: 25
GC content: 0.76
Affinity: Kd: 35 nM
Pool type: 5'-GGGAGGACGAUGCGG-N40/N60-CAGACGACUCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: PBS, modified to contain 1 mM of Mgz+ ions (138 mM NaCI, 2.7 mM KCI, 8.1 mM NA,HP04, 1.1 mM KH,P04, and 1 mM MgCI, pH 7.4)
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: 25-nucleotide truncate
Application as quoted in the referenced paper: Therapeutic: " Using the systematic evolution of ligands by exponential enrichment (SELEX) method, we have identified oligonucleotides that bind to human IgE with high affinities and high specificity. Therefore, these oligonucleotide ligands represent a novel class of IgE inhibitors that may prove useful in the fight against allergic diseases."
Additional information: SELEX experiments targeting human IgE were performed using 2'-NH, RNA
Reference (1996, J Immunol ): Wiegand, T. W., Williams, P. B., Dreskin, S. C., Jouvin, M. H., Kinet, J. P., & Tasset, D. (1996). High-affinity oligonucleotide ligands to human IgE inhibit binding to Fc epsilon receptor I. Journal of immunology (Baltimore, Md. : 1950), 157(1), 221–230.
Aptamer: D17.4
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5'GGGGCACGTTTATCCGTCCCTCCTAGTGGCGTGCCCC3'
Sequence length: 37
GC content: 0.6756756756756757
Affinity: Kd: 10 nM
Pool type: 5'-CTACCTACGATCTGACTAGC-N40-GCTTACTCTCATGTAGTTCC-3'
Pool random region: 40
Binding buffer/conditions: PBS, modified to contain 1 mM of Mgz+ ions (138 mM NaCI, 2.7 mM KCI, 8.1 mM NA,HP04, 1.1 mM KH,P04, and 1 mM MgCI, pH 7.4)
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: 37-nucleotide truncate
Application as quoted in the referenced paper: Therapeutic: " Using the systematic evolution of ligands by exponential enrichment (SELEX) method, we have identified oligonucleotides that bind to human IgE with high affinities and high specificity. Therefore, these oligonucleotide ligands represent a novel class of IgE inhibitors that may prove useful in the fight against allergic diseases."
Additional information: Consensus sequence: TTTATCCGTTCCTCTTAGTGG
Reference (1996, J Immunol ): Wiegand, T. W., Williams, P. B., Dreskin, S. C., Jouvin, M. H., Kinet, J. P., & Tasset, D. (1996). High-affinity oligonucleotide ligands to human IgE inhibit binding to Fc epsilon receptor I. Journal of immunology (Baltimore, Md. : 1950), 157(1), 221–230.
Aptamer: 14.12
Target: L-selectin receptor globulin (LS-Rg)
Type of nucleic acid: ssRNA
Sequence: 5'UCGGGCGAGUCGUCUGUAACAACAAUCAAGGCGGGUUCACCGCCCCAGUAUGAGUACCGCAUCGUCCUCCC3'
Sequence length: 71
GC content: 0.5915492957746479
Affinity: Kd: 0.2 nM and 3 nM to soluble L-selectin at 4°C and 22°C respectively
Pool type: 5'-TCGGGCGAGTCGTCTG-N40-CCGCATCGTCCTCCC-3'
Pool random region: 40
Binding buffer/conditions: 20 mM Hepes, pH 7.4, 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Application as quoted in the referenced paper: Therapeutic: " The selectins are calcium-dependent C-type lectins that recognize complex anionic carbohydrate ligands, initiating many cell-cell interactions in the vascular system. Selectin blockade shows therapeutic promise in a variety of inflammatory and postischemic pathologies.SELEX against recombinant L-selectin yielded calcium-dependent antagonists with ~10^5 higher affinity than the conventional oligosaccharide ligand sialyl Lewis^x. Moreover, they show calcium-dependent binding to native L-selectin on peripheral blood lymphocytes and block L-selectin-dependent interactions with the natural ligands on high endothelial venules."
Reference (1996, Proc Natl Acad Sci U S A ): O'Connell, D., Koenig, A., Jennings, S., Hicke, B., Han, H. L., Fitzwater, T., Chang, Y. F., Varki, N., Parma, D., & Varki, A. (1996). Calcium-dependent oligonucleotide antagonists specific for L-selectin. Proceedings of the National Academy of Sciences of the United States of America, 93(12), 5883–5887. https://doi.org/10.1073/pnas.93.12.5883
Aptamer: 13.32
Target: L-selectin receptor globulin (LS-Rg)
Type of nucleic acid: ssRNA
Sequence: 5'UCGGGCGAGUCGUCUGCGCGUAUGUGUGAAAGCGUGUGCACGGAGGCGUCUACAAUCCGCAUCGUCCUCCC3'
Sequence length: 71
GC content: 0.6197183098591549
Affinity: Kd: 4 nM and 3 nM to soluble L-selectin at 4°C and 22°C respectively
Pool type: 5'-TCGGGCGAGTCGTCTG-N40-CCGCATCGTCCTCCC-3'
Pool random region: 40
Binding buffer/conditions: 20 mM Hepes, pH 7.4, 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Application as quoted in the referenced paper: Therapeutic: " The selectins are calcium-dependent C-type lectins that recognize complex anionic carbohydrate ligands, initiating many cell-cell interactions in the vascular system. Selectin blockade shows therapeutic promise in a variety of inflammatory and postischemic pathologies.SELEX against recombinant L-selectin yielded calcium-dependent antagonists with ~10^5 higher affinity than the conventional oligosaccharide ligand sialyl Lewis^x. Moreover, they show calcium-dependent binding to native L-selectin on peripheral blood lymphocytes and block L-selectin-dependent interactions with the natural ligands on high endothelial venules."
Reference (1996, Proc Natl Acad Sci U S A ): O'Connell, D., Koenig, A., Jennings, S., Hicke, B., Han, H. L., Fitzwater, T., Chang, Y. F., Varki, N., Parma, D., & Varki, A. (1996). Calcium-dependent oligonucleotide antagonists specific for L-selectin. Proceedings of the National Academy of Sciences of the United States of America, 93(12), 5883–5887. https://doi.org/10.1073/pnas.93.12.5883
Aptamer: TQ30
Target: Thermus aquaticus DNA polymerase (Taq pol)
Type of nucleic acid: ssDNA
Sequence: 5'TTCTCGGTTGGTCTCTGGCGGAGCAAGACCAGACAATGTACAGTATTGGCCTGATCTTGTGTATGATTCGCTTTTCCC3'
Sequence length: 78
GC content: 0.4871794871794871
Affinity: Kd: 40 ± 1 pM
Pool type: 5'-TTCTCGGTTGGTCTCTGGCGGAGC-N30-TCTTGTGTATGATTCGCTTTTCCC-3'
Pool random region: 30
Binding buffer/conditions: 50 mMKCl, 2.5 mM MgCl2,10 mM Tris-HCl (pH 8.3 at 22°C)
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 8.3
Molecular weight of target: 94 kDa
Application as quoted in the referenced paper: Detection: " We show that the addition of oligonucleotide inhibitors eliminated the need for ‘‘hot start’’ conditions and improved the efficiency of detection of a low copy number target in PCR. The ability to detect very low copy number nucleic acid sequences, down to the single copy level has permitted the use of PCR in a vast number of applications including clinical diagnosis of infectious agents, cancer, genetic susceptibility to diseases, forensics and blood banking"
Reference (1996, J Mol Biol ): Dang, C., & Jayasena, S. D. (1996). Oligonucleotide inhibitors of Taq DNA polymerase facilitate detection of low copy number targets by PCR. Journal of molecular biology, 264(2), 268–278. https://doi.org/10.1006/jmbi.1996.0640
Aptamer: TQ30
Target: DNA polymerase (Taq pol), Thermus acquaticus
Type of nucleic acid: ssDNA
Sequence: 5'TTCTCGGTTGGTCTCTGGCGGAGCAAGACCAGACAATGTACAGTATTGGCCTGATCTTGTGTATGATTCGCTTTTCCC3'
Sequence length: 78
GC content: 0.4871794871794871
Affinity: Kd: 40 pM, IC50: 22 nM
Pool type: 5'-TTCTCGGTTGGTCTCTGGCGGAGC-N30-TCTTGTGTATGATTCGCTTTTCCC-3'
Pool random region: 30
Binding buffer/conditions: Not reported
Buffer type: Not Reported
Molecular weight of target: 94 kDa
Application as quoted in the referenced paper: Research- " These aptamers have been shown to effciently inhibit the polymerase activity of Taq pol and are useful in enhancing the amplifcation effciency of low copy number targets by the polymerase chain reaction (PCR). This aptamer inhibited Tth pol. This aptamer inhibited Stoffel fragment (61 kDa)"
Reference (1997, J Mol Biol ): Lin, Y., & Jayasena, S. D. (1997). Inhibition of multiple thermostable DNA polymerases by a heterodimeric aptamer. Journal of molecular biology, 271(1), 100–111. https://doi.org/10.1006/jmbi.1997.1165