RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: clone 6
Target: Fluorophore sulforhodamine B
Type of nucleic acid: ssDNA
Sequence: 5'CGGGATCCTAATGACCAAGGCCAAGGGAGGCTCCTTGTTATTCAGCAGGTACTACTATCTGGGAAAGAATCCCGAGTGTGTAGATGTTCCTGGGTGCCAGTCGGATAGTGTTCCTATAGTGAGTCGTATTAGAA3'
Sequence length: 134
GC content: 0.4850746268656716
Affinity: Not Reported 
Pool type: 5'-AACACTATCCGACTGGCACC-N72-CCTTGGTCATTAGGATCCCG-3'
Pool random region: 72
Binding buffer/conditions: 0.1 M KCI, 5 mM MgCI,, 10 mM Na-HEPES, pH 7.4
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Application as quoted in the referenced paper: Detection: " The incorporation of three-tiered G-quartet stacked on a duplex into other DNAs (e.g. PCR primers) could facilitate their in vitro labeling and detection. Mutagenesis followed by both negative and positive selection might yield specialized aptamers optimized for highly specific recognition of individual fluorophores. The current motif could be incorporated into larger random pools that could serve as the starting point for new selection experiments directed at the isolation of ribozymes that utilize fluorigenie substrates."
Reference (1998, Chem Biol ): Wilson, C., & Szostak, J. W. (1998). Isolation of a fluorophore-specific DNA aptamer with weak redox activity. Chemistry & biology, 5(11), 609–617. https://doi.org/10.1016/s1074-5521(98)90289-7
Aptamer: clone 26
Target: Fluorophore sulforhodamine B
Type of nucleic acid: ssDNA
Sequence: 5'CGGGATCCTAATGACCAAGGGGCGGGGGTGGTGGGAGTCGAGGTCATGGGTTCCCTGCGGTTGCGGCTCAGGCAAGACAAATCGATTAGAGCGGTGCCAGTCGGATAGTGTTCCTATAGTGAGTCGTATTAGAA3'
Sequence length: 134
GC content: 0.5597014925373134
Affinity: Not Reported 
Pool type: 5'-AACACTATCCGACTGGCACC-N72-CCTTGGTCATTAGGATCCCG-3'
Pool random region: 72
Binding buffer/conditions: 0.1 M KCI, 5 mM MgCI,, 10 mM Na-HEPES, pH 7.4
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Application as quoted in the referenced paper: Detection: " The incorporation of three-tiered G-quartet stacked on a duplex into other DNAs (e.g. PCR primers) could facilitate their in vitro labeling and detection. Mutagenesis followed by both negative and positive selection might yield specialized aptamers optimized for highly specific recognition of individual fluorophores. The current motif could be incorporated into larger random pools that could serve as the starting point for new selection experiments directed at the isolation of ribozymes that utilize fluorigenie substrates."
Reference (1998, Chem Biol ): Wilson, C., & Szostak, J. W. (1998). Isolation of a fluorophore-specific DNA aptamer with weak redox activity. Chemistry & biology, 5(11), 609–617. https://doi.org/10.1016/s1074-5521(98)90289-7
Aptamer: clone 39
Target: Fluorophore sulforhodamine B
Type of nucleic acid: ssDNA
Sequence: 5'CGGGATCCTAATGACCAAGGGTGGGGGGGAGTGGAGGTTATTAGGTTCAGTAGTGCCAACTGCAGTCTAAGCGCGTCGCGAGTACACCTTCTGGTGCCAGTCGGATAGTGTTCCTATAGTGAGTCGTATTAGAA3'
Sequence length: 134
GC content: 0.5223880597014925
Affinity: Not Reported 
Pool type: 5'-AACACTATCCGACTGGCACC-N72-CCTTGGTCATTAGGATCCCG-3'
Pool random region: 72
Binding buffer/conditions: 0.1 M KCI, 5 mM MgCI,, 10 mM Na-HEPES, pH 7.4
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Application as quoted in the referenced paper: Detection: " The incorporation of three-tiered G-quartet stacked on a duplex into other DNAs (e.g. PCR primers) could facilitate their in vitro labeling and detection. Mutagenesis followed by both negative and positive selection might yield specialized aptamers optimized for highly specific recognition of individual fluorophores. The current motif could be incorporated into larger random pools that could serve as the starting point for new selection experiments directed at the isolation of ribozymes that utilize fluorigenie substrates."
Reference (1998, Chem Biol ): Wilson, C., & Szostak, J. W. (1998). Isolation of a fluorophore-specific DNA aptamer with weak redox activity. Chemistry & biology, 5(11), 609–617. https://doi.org/10.1016/s1074-5521(98)90289-7
Aptamer: FB-1
Target: Fluorescein
Type of nucleic acid: ssRNA
Sequence: 5'GGACGGCACCACGGUCGGAUCCGUGAGUUGUGACAAUUUAGCGGGUGGUAUUAGAGCCUACUGCCACAGCAAUAGGAUCGAUACAGAUCU3'
Sequence length: 90
GC content: 0.5222222222222223
Affinity: Not reported
Pool type: 5'-AACACTATCCGACTGGCACC-N72-CCTTGGTCATTAGGATCCCG-3'
Pool random region: 72
Binding buffer/conditions: 100 mM KCl, 5 mM MgCl2, 10 mM Na-HEPES, pH 7.4
Divalent salt: MgCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Application as quoted in the referenced paper: Detection and Diagnostic: "In addition to serving as a model system for understanding the basis of RNA folding and function, these experiments demonstrate potential applications for the aptamers in transcript double labeling or fluorescence resonance energy transfer studies. Fluorophore-specific aptamers would also serve as a logical starting point for the evolution of ribozymes capable of utilizing fluorigenic substrates; such ribozymes could be readily introduced into diagnostic assays, possibly coupled directly with PCR-based detection methods."
Additional information: DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1998, Fold Des ): Holeman, L. A., Robinson, S. L., Szostak, J. W., & Wilson, C. (1998). Isolation and characterization of fluorophore-binding RNA aptamers. Folding & design, 3(6), 423–431. https://doi.org/10.1016/S1359-0278(98)00059-5
Aptamer: clone 7
Target: Nuclear factor for human IL6 (NF-IL6)
Type of nucleic acid: ssDNA
Sequence: 5‘CAGTGCTCTAGAGGATCCGTGACGGCCGACCGCACAGCACAACCCCGAAGCTTATCGATCCGAGCG3‘
Sequence length: 66
GC content: 0.6212121212121212
Affinity: Kobs < 2nM
Pool type: 5‘-CAGTGCTCTAGAGGATCCGTGAC-N22-CGAAGCTTATCGATCCGAGCG-3‘
Pool random region: 22
Binding buffer/conditions: 10 mM Tris, pH 7.5, 1 mM DTT, and 50−400 mM KCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: 18.926 Kda
Application as quoted in the referenced paper: Research: " These results demonstrate that oligonucleotide combinatorial methods can now be extended to selection not only of base sequence but also of phosphate (or monothiophosphate) backbones as well. Random combinatorial libraries and selection for aptamers with a much greater diversity of structures (7^N vs 4^N) are thus possible. Since thiophosphate substituted oligonucleotides show reduced nuclease activity and enhanced interaction with proteins in general, not just DNA binding proteins, this “thiophosphate-selection” experiment can offer wide application."
Reference (1998, Biochemistry ): King, D. J., Ventura, D. A., Brasier, A. R., & Gorenstein, D. G. (1998). Novel combinatorial selection of phosphorothioate oligonucleotide aptamers. Biochemistry, 37(47), 16489–16493. https://doi.org/10.1021/bi981780f
Aptamer: clone 8
Target: Nuclear factor for human IL6 (NF-IL6)
Type of nucleic acid: ssDNA
Sequence: 5‘CAGTGCTCTAGAGGATCCGTGACGGGCCCGCTGTACATGCACACGCGAAGCTTATCGATCCGAGCG3‘
Sequence length: 66
GC content: 0.6060606060606061
Affinity: Kobs < 2nM
Pool type: 5‘-CAGTGCTCTAGAGGATCCGTGAC-N22-CGAAGCTTATCGATCCGAGCG-3‘
Pool random region: 22
Binding buffer/conditions: 10 mM Tris, pH 7.5, 1 mM DTT, and 50−400 mM KCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: 18.926 Kda
Application as quoted in the referenced paper: Research: " These results demonstrate that oligonucleotide combinatorial methods can now be extended to selection not only of base sequence but also of phosphate (or monothiophosphate) backbones as well. Random combinatorial libraries and selection for aptamers with a much greater diversity of structures (7^N vs 4^N) are thus possible. Since thiophosphate substituted oligonucleotides show reduced nuclease activity and enhanced interaction with proteins in general, not just DNA binding proteins, this “thiophosphate-selection” experiment can offer wide application."
Reference (1998, Biochemistry ): King, D. J., Ventura, D. A., Brasier, A. R., & Gorenstein, D. G. (1998). Novel combinatorial selection of phosphorothioate oligonucleotide aptamers. Biochemistry, 37(47), 16489–16493. https://doi.org/10.1021/bi981780f
Aptamer: clone 13
Target: Nuclear factor for human IL6 (NF-IL6)
Type of nucleic acid: ssDNA
Sequence: 5‘CAGTGCTCTAGAGGATCCGTGACCCCGTTGTTGTCCCACTCCACGCGAAGCTTATCGATCCGAGCG3‘
Sequence length: 66
GC content: 0.5909090909090909
Affinity: Kobs < 2nM
Pool type: 5‘-CAGTGCTCTAGAGGATCCGTGAC-N22-CGAAGCTTATCGATCCGAGCG-3‘
Pool random region: 22
Binding buffer/conditions: 10 mM Tris, pH 7.5, 1 mM DTT, and 50−400 mM KCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: 18.926 Kda
Application as quoted in the referenced paper: Research: " These results demonstrate that oligonucleotide combinatorial methods can now be extended to selection not only of base sequence but also of phosphate (or monothiophosphate) backbones as well. Random combinatorial libraries and selection for aptamers with a much greater diversity of structures (7^N vs 4^N) are thus possible. Since thiophosphate substituted oligonucleotides show reduced nuclease activity and enhanced interaction with proteins in general, not just DNA binding proteins, this “thiophosphate-selection” experiment can offer wide application."
Reference (1998, Biochemistry ): King, D. J., Ventura, D. A., Brasier, A. R., & Gorenstein, D. G. (1998). Novel combinatorial selection of phosphorothioate oligonucleotide aptamers. Biochemistry, 37(47), 16489–16493. https://doi.org/10.1021/bi981780f
Aptamer: Cel#16
Target: Cellobiose
Type of nucleic acid: ssDNA
Sequence: 5′GCGGGGTTGGGCGGGTGGGTTCGCTGGGCAGGGGGCGAGTG	3'
Sequence length: 41
GC content: 0.7804878048780488
Affinity: Kd:  6 × 10−7 M
Pool type: 5′-ATAGGAGTCGACCGACCAGAA-N40-TATGTGCGTCTACATCTAGACTCAT-3'
Pool random region: 40
Binding buffer/conditions: 20 mM Tris, pH 7.5/100 mM NaCl/5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Trunction:  Only the internal variable region of three aptamers in were used: the 41 mer of Cel#16, the 40 mer of Cel#183, and the 36 mer of Cel#202
Application as quoted in the referenced paper: Detection: " The results of this study suggest it should be possible to develop specific aptamers against a wide array of carbohydrate antigens. This work demonstrates that relatively simple carbohydrate antigens are potential targets for highly selective DNA ligands, suggesting that it should be possible to select DNA, RNA, and modified polynucleotide aptamers from random sequence pools as recognition tools for a array of surface antigens."
Reference (1998, Proc Natl Acad Sci U S A ): Yang, Q., Goldstein, I. J., Mei, H. Y., & Engelke, D. R. (1998). DNA ligands that bind tightly and selectively to cellobiose. Proceedings of the National Academy of Sciences of the United States of America, 95(10), 5462–5467. https://doi.org/10.1073/pnas.95.10.5462
Aptamer: Cel#183
Target: Cellobiose
Type of nucleic acid: ssDNA
Sequence: 5′TAGCGGGTGTGGTGGGTGGGGGAGGCATGGTTTTTGGTAA3'
Sequence length: 40
GC content: 0.575
Affinity: Kd: 10−7 to 10−5 M
Pool type: 5′-ATAGGAGTCGACCGACCAGAA-N40-TATGTGCGTCTACATCTAGACTCAT-3'
Pool random region: 40
Binding buffer/conditions: 20 mM Tris, pH 7.5/100 mM NaCl/5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Trunction:  Only the internal variable region of three aptamers in were used: the 41 mer of Cel#16, the 40 mer of Cel#183, and the 36 mer of Cel#203
Application as quoted in the referenced paper: Detection: " The results of this study suggest it should be possible to develop specific aptamers against a wide array of carbohydrate antigens. This work demonstrates that relatively simple carbohydrate antigens are potential targets for highly selective DNA ligands, suggesting that it should be possible to select DNA, RNA, and modified polynucleotide aptamers from random sequence pools as recognition tools for a array of surface antigens."
Reference (1998, Proc Natl Acad Sci U S A ): Yang, Q., Goldstein, I. J., Mei, H. Y., & Engelke, D. R. (1998). DNA ligands that bind tightly and selectively to cellobiose. Proceedings of the National Academy of Sciences of the United States of America, 95(10), 5462–5467. https://doi.org/10.1073/pnas.95.10.5462
Aptamer: N30yc5
Target: Protein tyrosine phosphatases (PTPase) ( Yop51*Δ162 ), Yersinia
Type of nucleic acid: ssRNA
Sequence: 5'GGGAAUGGAUCCACAUCUACGUAUUACUGCUGGUGACGAGGGCUAGACGACGUACCUUCACUGCAGACUUGACGAAGCUU3'
Sequence length: 80
GC content: 0.5125
Affinity: Kd: 28 ± 12 nM
Pool type: 5'-GGGAATGGATCCACATCTACGTATTA-N30-TTCACTGCAGACTTGACGAAGCTT-3'
Pool random region: 30
Binding buffer/conditions: 20 mm Tris (pH 7.6), 150 mm NaCl, 5 mm MgCl2, 1 mm dithiothreitol; 100 μl final volume
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Application as quoted in the referenced paper: Drug Development: "The aptamers selected to bind Yop51 or other PTPase targets should prove useful for studying protein-protein interactions, dissecting the complex web of cellular signal transduction pathways, and developing novel pharmaceuticals."
Additional information: DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1998, J Biol Chem ): Bell, S. D., Denu, J. M., Dixon, J. E., & Ellington, A. D. (1998). RNA molecules that bind to and inhibit the active site of a tyrosine phosphatase. The Journal of biological chemistry, 273(23), 14309–14314. https://doi.org/10.1074/jbc.273.23.14309
Aptamer: N71yc16
Target: Protein tyrosine phosphatases (PTPase) ( Yop51*Δ162 ), Yersinia
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAUACCAGCUUAUUCAAUUCUGGCAAUGGGCUAUCCCAAGUGCUAGGCUUCAGGGAGCGAGGACCAGACGACGUACCUAACCCUAAGGUGAGAUAGUAAGUGCAAUCU3'
Sequence length: 112
GC content: 0.5
Affinity: Kd: 18 ± 2.9 nM
Pool type: 5'-GGGAGATACCAGCTTATTCAATT-N71-AGATAGTAAGTGCAATCT-3'
Pool random region: 71
Binding buffer/conditions: 20 mm Tris (pH 7.6), 150 mm NaCl, 5 mm MgCl2, 1 mm dithiothreitol; 100 μl final volume
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Application as quoted in the referenced paper: Drug Development: "The aptamers selected to bind Yop51 or other PTPase targets should prove useful for studying protein-protein interactions, dissecting the complex web of cellular signal transduction pathways, and developing novel pharmaceuticals."
Additional information: DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1998, J Biol Chem ): Bell, S. D., Denu, J. M., Dixon, J. E., & Ellington, A. D. (1998). RNA molecules that bind to and inhibit the active site of a tyrosine phosphatase. The Journal of biological chemistry, 273(23), 14309–14314. https://doi.org/10.1074/jbc.273.23.14309
Aptamer: 21.01
Target: Glutathione S-transferase-fused RBD (GST-RBD) Ras-binding domain (Raf-1 RBD)
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAUCAGAAUAAACGCUCAACUGAUCAAUGGCGUACAAUGGAUUCGUUCUCAUAACCAAAACCCUUACCCCUUGGACUGAUUCGACAUGAGGCCCCUGCAGGGCG3'
Sequence length: 108
GC content: 0.5
Affinity: Kd: 332 ± 93 nM
Pool type: 5'-GGGAGAUCAGAAUAAACGCUCAA-N60-UUCGACAUGAGGCCCCUGCAGGGCG-3'
Pool random region: 60
Binding buffer/conditions: Phosphate-buffered saline containing 5 mM MgCl2, buffer A
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 74 kDa
Application as quoted in the referenced paper: Drug Delivery: "The aptamers to the Raf-1 RBD may be used to specifically inhibit the Ras-Raf interaction in the complicated signaling network in cells, without affecting other downstream effectors of Ras. The anti-Raf-1 aptamers would be delivered through the cell membrane, or transiently expressed in the cell, as reported for RNA aptamers to HIV Rev. The anti-Raf1 aptamers may also be potential diagnostic and therapeutic tools targeting the interaction between two proto-oncogene products, Ras and Raf-1, as in the cases of aptamers to human neutrophil elastase used in in vivo imaging of infammation and in RNA therapy for lung infammatory injury."
Reference (1998, FEBS Lett ): Kimoto, M., Sakamoto, K., Shirouzu, M., Hirao, I., & Yokoyama, S. (1998). RNA aptamers that specifically bind to the Ras-binding domain of Raf-1. FEBS letters, 441(2), 322–326. https://doi.org/10.1016/s0014-5793(98)01572-5
Aptamer: 21.07
Target: Glutathione S-transferase-fused RBD (GST-RBD) Ras-binding domain (Raf-1 RBD)
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAUCAGAAUAAACGCUCAAUUGACUCAAUGGCGUACAAUGGAUUCGUUCUCAUAACCAAAACCCUUACCCCUUGGACUGUUCGACAUGAGGCCCCUGCAGGGCG3'
Sequence length: 108
GC content: 0.5
Affinity: Kd: 332 ± 93 nM
Pool type: 5'-GGGAGAUCAGAAUAAACGCUCAA-N60-UUCGACAUGAGGCCCCUGCAGGGCG-3'
Pool random region: 60
Binding buffer/conditions: Phosphate-buffered saline containing 5 mM MgCl2, buffer A
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 74 kDa
Application as quoted in the referenced paper: Drug Delivery: "The aptamers to the Raf-1 RBD may be used to specifically inhibit the Ras-Raf interaction in the complicated signaling network in cells, without affecting other downstream effectors of Ras. The anti-Raf-1 aptamers would be delivered through the cell membrane, or transiently expressed in the cell, as reported for RNA aptamers to HIV Rev. The anti-Raf1 aptamers may also be potential diagnostic and therapeutic tools targeting the interaction between two proto-oncogene products, Ras and Raf-1, as in the cases of aptamers to human neutrophil elastase used in in vivo imaging of infammation and in RNA therapy for lung infammatory injury."
Reference (1998, FEBS Lett ): Kimoto, M., Sakamoto, K., Shirouzu, M., Hirao, I., & Yokoyama, S. (1998). RNA aptamers that specifically bind to the Ras-binding domain of Raf-1. FEBS letters, 441(2), 322–326. https://doi.org/10.1016/s0014-5793(98)01572-5
Aptamer: J6f1
Target: Tobramycin
Type of nucleic acid: ssRNA
Sequence: 5'GGCUUAGUAUAGCGAGGUUUAGCUACACUCGUGCUGAGCC3'
Sequence length: 40
GC content: 0.525
Affinity: Kd: 5.15 ± 1.52 nM
Pool type: 5'-GGGAGAAUUCCGACCAGAAGCUU-N60-CAUAUGUGCGUCUACAUGGAUCCUCA-3'
Pool random region: 60
Binding buffer/conditions: Not reported
Buffer type: Not Reported
Molecular weight of target: Not reported
Post-SELEX modifications: Truncated version of previously selected j6 aptamer against tobramycin
Application as quoted in the referenced paper: Therapeutic and Research: "An understanding of the rules underlying RNA-aminoglycoside recognition would be extremely useful as a basis for the design of potent and selective antagonists of RNA function."
Additional information: pool is from (Wang & Rando, 1995)
Reference (1998, Biochemistry ): Hamasaki, K., Killian, J., Cho, J., & Rando, R. R. (1998). Minimal RNA constructs that specifically bind aminoglycoside antibiotics with high affinities. Biochemistry, 37(2), 656–663. https://doi.org/10.1021/bi971095t
Aptamer: ps11-20
Target: Recombinant basic fibroblast growth factor (bFGF), Human
Type of nucleic acid: ssRNA
Sequence: 5'GGGAAUGGAUCCACAUCUACGAAUUCAAUCCCAAUGGCUUGAACUGCCAACGAACGUUCACUGCAGACUUGACGAAGCUU3'
Sequence length: 80
GC content: 0.475
Affinity: Kd: 1.8 ±  0.8 nM
Pool type: 5'-GGGAATGGATCCACATCTACGAATTC-N30-TTCACTGCAGACTTGACGAAGCTT3'
Pool random region: 30
Binding buffer/conditions: PBS, phosphate buffered saline (101 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaC1, 2.7 mM KC1) pH 7.4,
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Application as quoted in the referenced paper: Research: " The aptamer may be able to identify related heparin binding sites and discriminate against nonrelated heparin binding sites; the basis for this discrimination may be the aptamer's mimicry of one of several different natural sulfated oligosaccharides of which heparin is a generic example."
Additional information: *inconsistencies in reporting RNA aptamer as DNA, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
In particular, RNA pools  that contained 2" modified pyrimidines have been used to identify aptarners that can bind tightly and specifically to vascular endothelial growth factor (VEGF), bFGF, and keratinocyte growth factor (KGF). 4-6 Modified  nucleotides can also be added following selection. For example, caps containing deoxynucleotides with phosphorothioate linkages were appended to both ends of an anti-VEGF aptamer and stabilized the aptamer to exonuclease degradation. 4
Reference (1998, Bioorg Med Chem Lett ): Jhaveri, S., Olwin, B., & Ellington, A. D. (1998). In vitro selection of phosphorothiolated aptamers. Bioorganic & medicinal chemistry letters, 8(17), 2285–2290. https://doi.org/10.1016/s0960-894x(98)00414-4
Aptamer: C #128 (46 mer)
Target: Streptomycin
Type of nucleic acid: ssRNA
Sequence: 5'GGAUCGCAUUUGGACUUCUGCCCAGGGUGGCACCACGGUCGGAUCC3'
Sequence length: 46
GC content: 0.6304347826086957
Affinity: Not reported
Pool type: 5'-GGAGCUCAGCCUUCACUGC-N74-GGCACCACGGUCGGAUCCAC3'
Pool random region: 74
Binding buffer/conditions: 5 mM MgCl2, 50 mM Tris-HCl, pH 7.6, 250 mM NaCl
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Pb21-induced cleavage of c #128
Application as quoted in the referenced paper: Drug Development: "A growing body of evidence points to RNA as a crucial target for antibacterial and antiviral drugs. For example, the aminocyclitol antibiotic streptomycin interacts with the 16S ribosomal RNA and, in addition, inhibits group I intron splicing. To understand the mode of binding of streptomycin to RNA, we isolated small, streptomycin-binding RNA aptamers via in vitro selection"
Reference (1998, RNA ): Wallace, S. T., & Schroeder, R. (1998). In vitro selection and characterization of streptomycin-binding RNAs: recognition discrimination between antibiotics. RNA (New York, N.Y.), 4(1), 112–123.
Aptamer: B # 84
Target: Streptomycin
Type of nucleic acid: ssRNA
Sequence: 5'GGAGCUCAGCCUUCACUGCCAGACAGUAGAGGGAAGUGUGAGCUAUCACCUCAAGGAAAACGCUUCAGAAAGGGACUUAGGUGAUGAUAGUGUGGCACCACGGUCGGAUCCAC3'
Sequence length: 113
GC content: 0.5309734513274337
Affinity: Not reported
Pool type: 5'-GGAGCUCAGCCUUCACUGC-N74-GGCACCACGGUCGGAUCCAC3'
Pool random region: 74
Binding buffer/conditions: 5 mM MgCl2, 50 mM Tris-HCl, pH 7.6, 250 mM NaCl
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Application as quoted in the referenced paper: Drug Development: "A growing body of evidence points to RNA as a crucial target for antibacterial and antiviral drugs. For example, the aminocyclitol antibiotic streptomycin interacts with the 16S ribosomal RNA and, in addition, inhibits group I intron splicing. To understand the mode of binding of streptomycin to RNA, we isolated small, streptomycin-binding RNA aptamers via in vitro selection"
Reference (1998, RNA ): Wallace, S. T., & Schroeder, R. (1998). In vitro selection and characterization of streptomycin-binding RNAs: recognition discrimination between antibiotics. RNA (New York, N.Y.), 4(1), 112–123.