RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: AFLA5
Target: Aflatoxin B1 (AFB1)
Type of nucleic acid: ssDNA
Sequence: 5'CTCGTCTCGTTCTCTCAGTCTTCTTCTGGCTTGGTGGTTGGTGTGTCTGCTGATTTGGTAGACACGAAGAAGAAGGAGGA3'
Sequence length: 80
GC content: 0.5
Affinity: Kd: 50.45 ± 11.06 nM
Pool type: 5'-CTCGTCTCGTTCTCTCAGTC-N40-GACACGAAGAAGAAGGAGGA-3'
Pool random region: 40
Binding buffer/conditions: 1X binding buffer (1X BB; 5 mM MgCl2 in Dulbecco's phosphate buffered saline, pH 7.4 ± 0.2)
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aflatoxins are naturally occurring mycotoxins that contaminate food and agro commodities, leading to acute and chronic health conditions in human and animals. In the present work, an attempt was made to generate high-affinity single stranded DNA aptamers that specifically bind to Aflatoxin B1 (AFB1) by a modified Systemic Evolution of Ligands by Exponential Enrichment (SELEX) procedure with the aid of Immunoaffinity columns. These findings indicate, immunoaffinity based SELEX can pave an alternative approach to screen aptamers against mycotoxin detection and purification."
Reference (2016, Front Microbiol ): Setlem, K., Mondal, B., Ramlal, S., & Kingston, J. (2016). Immuno Affinity SELEX for Simple, Rapid, and Cost-Effective Aptamer Enrichment and Identification against Aflatoxin B1. Frontiers in microbiology, 7, 1909. https://doi.org/10.3389/fmicb.2016.01909
Aptamer: AFLA71
Target: Aflatoxin B1 (AFB1)
Type of nucleic acid: ssDNA
Sequence: 5'CTCGTCTCGTTCTCTCAGTCGGACGAAGAGAGGGGGAGAGGGGGACGGAGCTGCTAAGGTGACACGAAGAAGAAGGAGGA3'
Sequence length: 80
GC content: 0.5875
Affinity: Kd: 85.02 ± 25.74 nM
Pool type: 5'-CTCGTCTCGTTCTCTCAGTC-N40-GACACGAAGAAGAAGGAGGA-3'
Pool random region: 40
Binding buffer/conditions: 1X binding buffer (1X BB; 5 mM MgCl2 in Dulbecco's phosphate buffered saline, pH 7.4 ± 0.2)
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aflatoxins are naturally occurring mycotoxins that contaminate food and agro commodities, leading to acute and chronic health conditions in human and animals. In the present work, an attempt was made to generate high-affinity single stranded DNA aptamers that specifically bind to Aflatoxin B1 (AFB1) by a modified Systemic Evolution of Ligands by Exponential Enrichment (SELEX) procedure with the aid of Immunoaffinity columns. These findings indicate, immunoaffinity based SELEX can pave an alternative approach to screen aptamers against mycotoxin detection and purification."
Reference (2016, Front Microbiol ): Setlem, K., Mondal, B., Ramlal, S., & Kingston, J. (2016). Immuno Affinity SELEX for Simple, Rapid, and Cost-Effective Aptamer Enrichment and Identification against Aflatoxin B1. Frontiers in microbiology, 7, 1909. https://doi.org/10.3389/fmicb.2016.01909
Aptamer: LY-1
Target: Hepatocellular carcinoma HCCLM9 (LY-1)
Type of nucleic acid: ssDNA
Sequence: 5′ATCCAGAGTGACGCAGCATTGGGTGTTAGGCTGGTCTTAATCGGGTCGGGTTGCTGTGGACACGGTGGCTTAGT3′
Sequence length: 74
GC content: 0.5540540540540541
Affinity: Kd: 167.3 ± 30.2
Pool type: 5′-ATCCAGAGTGACGCAGCA-N40-TGGACACGGTGGCTTAGT-3′
Pool random region: 40
Binding buffer/conditions: (PBS-1M MgCl2-0.1 mg/mL yeast tRNA-1 mg/mL BSA-0.1 mg/mL Salmon sperm DNA)
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: " Hepatocellular carcinoma (HCC) is one of the most deadly human cancers due to its ability of invasion and metastasis. In the present study, we used HCCLM9 cells with high metastatic potential and MHCC97L with low metastatic potential as a model system to study the molecular mechanisms of HCC metastasis. QD605 labeled LY-1 aptamer could recognize HCC cells in both local liver cancer tissues and pulmonary metastatic sites in a xenograft model of HCC with pulmonary metastasis. Taken together, the present study demonstrated the tumor targeting and tumor suppressive effects of LY-1, which could be a promising molecular probe for metastatic HCC and a potential candidate of chemotherapy for metastatic HCC."
Reference (2016, Oncotarget ): Rong, Y., Chen, H., Zhou, X. F., Yin, C. Q., Wang, B. C., Peng, C. W., Liu, S. P., & Wang, F. B. (2016). Identification of an aptamer through whole cell-SELEX for targeting high metastatic liver cancers. Oncotarget, 7(7), 8282–8294. https://doi.org/10.18632/oncotarget.6988
Aptamer: MRP1Apt
Target: Extracellular exposed motif of multidrug resistant-associated protein 1 (MRP1)
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5′GGGGAAUUCGGAGAGGGAGAAUAGUCAACAAAUCGUUUGGGGCGACUUCUCCUUCCUUUCUCCCUUCUCCC3′
Sequence length: 71
GC content: 0.5211267605633803
Affinity: Kd: 50 nM
Pool type: 5′-GGGGAATTCGGAGAGGGAGAATAG-N25-CTTCCTTTCTCCCTTCTCCC-3′
Pool random region: 25
Binding buffer/conditions: 150 mmol/l NaCl, 2 mmol/l CaCl2, 20 mmol/l HEPES (pH 7.4), 0,01% BSA binding buffer
Divalent salt: CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " In this work we develop the first aptamer that targets chemotherapy-resistant tumors expressing MRP1 through a novel combinatorial peptide-cell SELEX. With the use of the MRP1 aptamer we engineer a MRP1-CD28 bivalent aptamer that is able to bind MRP1-expressing tumors and deliver the CD28 costimulatory signal to tumor-infiltrating lymphocytes. The bi-specific aptamer is able to enhance costimulation in chemotherapy-resistant tumors. CD28Aptvax made of irradiated tumor cells coated with the CD28-agonistic aptamer attached to MRP1 elicits a strong tumor- cell immune response against melanoma tumors reducing tumor growth."
Additional information: DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.

Reference (2016, Oncotarget ): Soldevilla, M. M., Villanueva, H., Casares, N., Lasarte, J. J., Bendandi, M., Inoges, S., López-Díaz de Cerio, A., & Pastor, F. (2016). MRP1-CD28 bi-specific oligonucleotide aptamers: target costimulation to drug-resistant melanoma cancer stem cells. Oncotarget, 7(17), 23182–23196. https://doi.org/10.18632/oncotarget.8095
Aptamer: MRP1-CD28 bi-specific oligonucleotide aptamers
Target: Extracellular exposed motif of multidrug resistant-associated protein 1 (MRP1)
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5′GGGAGACCCACCCAUAAAAGGGAGAGAGGAAGAGGGAUGGGGAUUAGACCAUAGGCUCCCAACCCCCAUAACCCAGAGGUCGAUAGUACUGGAUCCCCCCGGGAGAGAGGAAGAGGGAUGGGGAUUAGAAUAGGCUCCCAACCCCCCAUAACCCAGAGGUCGAUAGUACUGGAUCCCGGGAAAUAUGGGUGGGUCUCCCGGGAGAAUAGUCAACAAAUCGUUUGGGGCGACUUCUCCUUCCUUUCUCCUGU3'
Sequence length: 251
GC content: 0.549800796812749
Affinity: Kd: 50 nM
Pool type: 5′-GGGGAATTCGGAGAGGGAGAATAG-N25-CTTCCTTTCTCCCTTCTCCC-3′
Pool random region: 25
Binding buffer/conditions: 150 mmol/l NaCl, 2 mmol/l CaCl2, 20 mmol/l HEPES (pH 7.4), 0,01% BSA binding buffer
Divalent salt: CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " In this work we develop the first aptamer that targets chemotherapy-resistant tumors expressing MRP1 through a novel combinatorial peptide-cell SELEX. With the use of the MRP1 aptamer we engineer a MRP1-CD28 bivalent aptamer that is able to bind MRP1-expressing tumors and deliver the CD28 costimulatory signal to tumor-infiltrating lymphocytes. The bi-specific aptamer is able to enhance costimulation in chemotherapy-resistant tumors. CD28Aptvax made of irradiated tumor cells coated with the CD28-agonistic aptamer attached to MRP1 elicits a strong tumor- cell immune response against melanoma tumors reducing tumor growth."
Additional information: DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
The sequence was extracted from the figure 3. The very top of figure 3 has not been shown (the hirpin loop), it was assumed there is no nucleotide
MRP1-CD28 bi-specific aptamer: CD28 aptamer, linker, MRP1 aptamer
Reference (2016, Oncotarget ): Soldevilla, M. M., Villanueva, H., Casares, N., Lasarte, J. J., Bendandi, M., Inoges, S., López-Díaz de Cerio, A., & Pastor, F. (2016). MRP1-CD28 bi-specific oligonucleotide aptamers: target costimulation to drug-resistant melanoma cancer stem cells. Oncotarget, 7(17), 23182–23196. https://doi.org/10.18632/oncotarget.8095
Aptamer: 12L12A
Target: Group A streptococcus (GAS) serotype M3
Type of nucleic acid: ssDNA
Sequence: 5'GCCTGTTGTGAGCCTCCTAACGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATACATGCTTATTCTTGTCTCC3'
Sequence length: 78
GC content: 0.5
Affinity: Kd: 8.25 ± 1.43 pM
Pool type: 5′-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3′
Pool random region: 38
Binding buffer/conditions: Phosphate-buffered saline (PBS) and 0.05% Tween 20, 1% bovine serum albumin
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Biosensor: " Group A streptococcus (GAS) is an important Gram-positive pathogen that causes various human diseases ranging from peripheral lesions to invasive infections. The M protein is one of the main virulence factors present on the cell surface and is associated with invasive GAS infections. In this study DNA aptamers with a high binding affinity towards S. pyogenes serotype M3 were selected through 12 iterative rounds of the Systematic Evolution of Ligands by EXponential (SELEX) enrichment. The isolated aptamer can be used in any tool, such as a biosensor, for the detection of S. pyogenes and can be used in the development of a novel M-typing system."
Reference (2016, Can J Microbiol ): Alfavian, H., Mousavi Gargari, S. L., Rasoulinejad, S., & Medhat, A. (2017). Development of a DNA aptamer that binds specifically to group A Streptococcus serotype M3. Canadian journal of microbiology, 63(2), 160–168. https://doi.org/10.1139/cjm-2016-0495
Aptamer: 12L18A
Target: Group A streptococcus (GAS) serotype M4
Type of nucleic acid: ssDNA
Sequence: 5'GCCTGTTGTGAGCCTCCTAACTCCTCGAGGGGGGGGGGATGAAAAGGAAAACGCAACAACATGCTTATTCTTGTCTCC3'
Sequence length: 78
GC content: 0.5256410256410257
Affinity: Kd: 7.47 ± 1.72 pM
Pool type: 5′-GCCTGTTGTGAGCCTCCTAAC-N38-CATGCTTATTCTTGTCTCC-3′
Pool random region: 38
Binding buffer/conditions: Phosphate-buffered saline (PBS) and 0.05% Tween 20, 1% bovine serum albumin
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Biosensor: " Group A streptococcus (GAS) is an important Gram-positive pathogen that causes various human diseases ranging from peripheral lesions to invasive infections. The M protein is one of the main virulence factors present on the cell surface and is associated with invasive GAS infections. In this study DNA aptamers with a high binding affinity towards S. pyogenes serotype M3 were selected through 12 iterative rounds of the Systematic Evolution of Ligands by EXponential (SELEX) enrichment. The isolated aptamer can be used in any tool, such as a biosensor, for the detection of S. pyogenes and can be used in the development of a novel M-typing system."
Reference (2016, Can J Microbiol ): Alfavian, H., Mousavi Gargari, S. L., Rasoulinejad, S., & Medhat, A. (2017). Development of a DNA aptamer that binds specifically to group A Streptococcus serotype M3. Canadian journal of microbiology, 63(2), 160–168. https://doi.org/10.1139/cjm-2016-0495
Aptamer: ER_Apt2
Target: Estrogen receptor alpha (ERα)
Type of nucleic acid: ssDNA
Sequence: 5′ATACCAGTCTATTCAATTTAATTAAGCTAGTGGGTGCTCTTAACGGAAGCATATCCCGAGATAGTATGTGCAATCA3′
Sequence length: 76
GC content: 0.3815789473684211
Affinity: Not reported
Pool type: 5′-ATACCAGTCTATTCAATT–N40-AGATAGTATGTGCAATCA- 3′
Pool random region: 40
Binding buffer/conditions: 20-mM Tris-Cl, pH 7.5, 120-mM NaCl, 5-mM KCl, 1-mM MgCl2, and 1-mM CaCl2
Divalent salt: MgCl/CaCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: 67 kDa
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: " Estrogen receptor alpha (ERα) also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1) is a ligand-activated transcription factor. It is an important biomarker for breast cancer metastasis. In the present study, we report a novel DNA aptamer candidate against estrogen receptor (ER) alpha structure. Cytochemical and immunohistochemistry assay indicates its potential use as a diagnostic agent against ERα positive carcinomas. The nucleotide aptamer sequences described in the present study can be used for the detection, treatment, prophylaxis and diagnosis of ERα-related disorder."
Reference (2016, Transl Res ): Sett, A., Borthakur, B. B., Sharma, J. D., Kataki, A. C., & Bora, U. (2017). DNA aptamer probes for detection of estrogen receptor α positive carcinomas. Translational research : the journal of laboratory and clinical medicine, 183, 104–120.e2. https://doi.org/10.1016/j.trsl.2016.12.008
Aptamer: BLG14
Target: Beta-lactoglobulin A
Type of nucleic acid: ssDNA
Sequence: 5'ATACCAGCTTATTCAATTCGACGATCGGACCGCAGTACCCACCCACCAGCCCCAACATCATGCCCATCCGTGTGTGAGATAGTAAGTGCAATCT3'
Sequence length: 94
GC content: 0.5106382978723404
Affinity: Kd: 82 ± 30 nM
Pool type: 5'-ATACCAGCTTATTCAATT-N60-AGATAGTAAGTGCAATCT-3'
Pool random region: 60
Binding buffer/conditions: 50 mM Tris, pH 7.5, 150 mM NaCl, 2 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Biosensor and Detection: " Food allergy has increased rapidly in recent years affecting millions of people worldwide. In this work, we developed an aptamer/graphene-based electrochemical biosensor for on-step, sensitive and low cost detection of β-lactoglobulin (β-LG) milk protein, one of the most common food allergens specially in infants. The integration of the novel aptamer in the graphene biosensor allows a promising way for cost-effective, rapid and sensitive on-site detection of milk allergen in food stuff."
Reference (2016, Biosens Bioelectron ): Eissa, S., & Zourob, M. (2017). In vitro selection of DNA aptamers targeting β-lactoglobulin and their integration in graphene-based biosensor for the detection of milk allergen. Biosensors & bioelectronics, 91, 169–174. https://doi.org/10.1016/j.bios.2016.12.020
Aptamer: BLG14
Target: Beta-lactoglobulin B
Type of nucleic acid: ssDNA
Sequence: 5'ATACCAGCTTATTCAATTCGACGATCGGACCGCAGTACCCACCCACCAGCCCCAACATCATGCCCATCCGTGTGTGAGATAGTAAGTGCAATCT3'
Sequence length: 94
GC content: 0.5106382978723404
Affinity: Kd: 80 ± 26 nM
Pool type: 5'-ATACCAGCTTATTCAATT-N60-AGATAGTAAGTGCAATCT-3'
Pool random region: 60
Binding buffer/conditions: 50 mM Tris, pH 7.5, 150 mM NaCl, 2 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Biosensor and Detection: " Food allergy has increased rapidly in recent years affecting millions of people worldwide. In this work, we developed an aptamer/graphene-based electrochemical biosensor for on-step, sensitive and low cost detection of β-lactoglobulin (β-LG) milk protein, one of the most common food allergens specially in infants. The integration of the novel aptamer in the graphene biosensor allows a promising way for cost-effective, rapid and sensitive on-site detection of milk allergen in food stuff."
Reference (2016, Biosens Bioelectron ): Eissa, S., & Zourob, M. (2017). In vitro selection of DNA aptamers targeting β-lactoglobulin and their integration in graphene-based biosensor for the detection of milk allergen. Biosensors & bioelectronics, 91, 169–174. https://doi.org/10.1016/j.bios.2016.12.020
Aptamer: G2
Target: Japanese encephalitis virus methyltransferase (JEV MTase)
Type of nucleic acid: 2'-O-Me-RNA
Sequence: 5′GGGAGAGCGGAAGCGUGCUGGGCCCCACGACAGCAUGCCAAUAGAUGCGCAUGGAGACGACAGCAUCAUAACCCAGAGGUCGAUGGAUCCCC3'
Sequence length: 92
GC content: 0.6086956521739131
Affinity: Kd: 12.78 ± 1.907 nM
Pool type: 5′-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3′
Pool random region: 40
Binding buffer/conditions: 30 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1.5 mM MgCl2, 2 mM dithiothreitol, 1% BSA
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Truncated
Application as quoted in the referenced paper: Therapeutic: " Japanese encephalitis virus (JEV) is the most common etiological agent of epidemic viral encephalitis. JEV MTase is essential for viral replication and specifically catalyzes methylation of the viral RNA cap, which occurs exclusively in the cytoplasm. Therefore, JEV MTase is a potential target for antiviral therapy. Here, we identified specific and avid RNA aptamer (Kd ∼ 12 nM) with modified 2'-O-methyl pyrimidines against JEV MTase. The 24-mer aptamer efficiently inhibited JEV production and replication in cells. Therefore, MTase-specific RNA aptamer might be useful as an anti-JEV agent."
Additional information: 2′-O-methyl pyrimidine nucleotides, 2'-hydroxyl purine nucleotides. Generated rnase-resistant 2′-o-methyl modified rna aptamer using mutant t7 rna polymerase that can incorporate 2′-o-methyl pyrimidine (c/u) nucleotides into in vitro transcribed rna [8]. The selected 2′-modified rna aptamer specifically bound to jev mtase with high affinity and efficiently inhibited its mtase activity.
Reference (2016, Biochem Biophys Res Commun ): Han, S. R., & Lee, S. W. (2017). Inhibition of Japanese encephalitis virus (JEV) replication by specific RNA aptamer against JEV methyltransferase. Biochemical and biophysical research communications, 483(1), 687–693. https://doi.org/10.1016/j.bbrc.2016.12.081
Aptamer: G2
Target: Japanese encephalitis virus methyltransferase (JEV NS5)
Type of nucleic acid: 2'-O-Me-RNA
Sequence: 5′GGGAGAGCGGAAGCGUGCUGGGCCCCACGACAGCAUGCCAAUAGAUGCGCAUGGAGACGACAGCAUCAUAACCCAGAGGUCGAUGGAUCCCC3′
Sequence length: 92
GC content: 0.6086956521739131
Affinity: Kd: 11.04 ± 1.763 nM
Pool type: 5′-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCC-3′
Pool random region: 40
Binding buffer/conditions: 31 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1.5 mM MgCl2, 2 mM dithiothreitol, 1% BSA
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Truncated
Application as quoted in the referenced paper: Therapeutic: " Japanese encephalitis virus (JEV) is the most common etiological agent of epidemic viral encephalitis. JEV MTase is essential for viral replication and specifically catalyzes methylation of the viral RNA cap, which occurs exclusively in the cytoplasm. Therefore, JEV MTase is a potential target for antiviral therapy. Here, we identified specific and avid RNA aptamer (Kd ∼ 12 nM) with modified 2'-O-methyl pyrimidines against JEV MTase. The 24-mer aptamer efficiently inhibited JEV production and replication in cells. Therefore, MTase-specific RNA aptamer might be useful as an anti-JEV agent."
Additional information: 2′-O-methyl pyrimidine nucleotides, 2'-hydroxyl purine nucleotides. Generated rnase-resistant 2′-o-methyl modified rna aptamer using mutant t7 rna polymerase that can incorporate 2′-o-methyl pyrimidine (c/u) nucleotides into in vitro transcribed rna [8]. The selected 2′-modified rna aptamer specifically bound to jev mtase with high affinity and efficiently inhibited its mtase activity.
Reference (2016, Biochem Biophys Res Commun ): Han, S. R., & Lee, S. W. (2017). Inhibition of Japanese encephalitis virus (JEV) replication by specific RNA aptamer against JEV methyltransferase. Biochemical and biophysical research communications, 483(1), 687–693. https://doi.org/10.1016/j.bbrc.2016.12.081
Aptamer: B5
Target: Salmonella typhimurium (S. typhimurium)
Type of nucleic acid: ssDNA
Sequence: 5'CAGTCCAGGACAGATTCGCGAGCCCACTCCAAACACGACCAACTCACGCTCTATCAACATCGCTATCCACGTGGATTTCATTCAGCGATT3'
Sequence length: 90
GC content: 0.5111111111111111
Affinity: Kd: 58.5 nM
Pool type: 5′-CAGTCCAGGACAGATTCGCGAG-N45-CACGTGGATTTCATTCAGCGATT-3'
Pool random region: 45
Binding buffer/conditions: 50 mM Tris-HCl, pH 7.5, 25 mM NaCl, 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: 5′-amino modification and a six carbon spacer. Then amino-modified aptamer was immobilized on the electrode surface for label-free detection of S. typhimurium.
Application as quoted in the referenced paper: Diagnostic and Detection: " In this study, quartz crystal microbalance (QCM) was used to select aptamers against Salmonella typhimurium. A QCM-based aptasensor was developed to detect S. typhimurium. This aptasensor was able to detect 103CFU/mL of S. typhimurium with less than 1h. This study demonstrated QCM-based selection could be more effective selection of aptamers and QCM-based aptasensor could be more sensitive in detecting S. typhimurium."
Reference (2016, Food Chem ): Wang, L., Wang, R., Chen, F., Jiang, T., Wang, H., Slavik, M., Wei, H., & Li, Y. (2017). QCM-based aptamer selection and detection of Salmonella typhimurium. Food chemistry, 221, 776–782. https://doi.org/10.1016/j.foodchem.2016.11.104
Aptamer: A24
Target: Salmonella typhimurium (S. typhimurium)
Type of nucleic acid: ssDNA
Sequence: 5'CAGTCCAGGACAGATTCGCGAGGCACAATCCACCTCTCACCGCACGCCACGCACTGCCTCTGTCCCGCACGTGGATTTCATTCAGCGATT3'
Sequence length: 90
GC content: 0.5888888888888889
Affinity: Kd: 19.63 nM
Pool type: 5′-CAGTCCAGGACAGATTCGCGAG-N45-CACGTGGATTTCATTCAGCGATT-3'
Pool random region: 45
Binding buffer/conditions: 51 mM Tris-HCl, pH 7.5, 25 mM NaCl, 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: 5′-amino modification and a six carbon spacer. Then amino-modified aptamer was immobilized on the electrode surface for label-free detection of S. typhimurium.
Application as quoted in the referenced paper: Diagnostic and Detection: " In this study, quartz crystal microbalance (QCM) was used to select aptamers against Salmonella typhimurium. A QCM-based aptasensor was developed to detect S. typhimurium. This aptasensor was able to detect 103CFU/mL of S. typhimurium with less than 1h. This study demonstrated QCM-based selection could be more effective selection of aptamers and QCM-based aptasensor could be more sensitive in detecting S. typhimurium."
Reference (2016, Food Chem ): Wang, L., Wang, R., Chen, F., Jiang, T., Wang, H., Slavik, M., Wei, H., & Li, Y. (2017). QCM-based aptamer selection and detection of Salmonella typhimurium. Food chemistry, 221, 776–782. https://doi.org/10.1016/j.foodchem.2016.11.104