RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: 20A24P
Target: Group A Streptococcus (GAS)*
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.575
Affinity: Kd: 9.1 ± 0.6 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 1× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic  and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."
Additional information: This aptamer was noted as "particulalry promising." A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. One of the recommended aptamers for this article. 5' fam addition
Reference (2011, Anal Chem ): Hamula, C. L., Le, X. C., & Li, X. F. (2011). DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes. Analytical chemistry, 83(10), 3640–3647. https://doi.org/10.1021/ac200575e
Aptamer: 15A3P
Target: Group A Streptococcus (GAS)*
Type of nucleic acid: ssDNA
Sequence: 5'TTCACGGTAGCACGCATAGGGACAGCAAGCCCAAGCTGGGTGTGCAAGGTGAGGAGTGGGCATCTGACCTCTGTGCTGCT3'
Sequence length: 80
GC content: 0.5875
Affinity: Kd: 9.6 ± 0.3 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 1× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic  and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."
Additional information: This aptamer was noted as "particulalry promising." A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. One of the recommended aptamers for this article. 5' fam addition
Reference (2011, Anal Chem ): Hamula, C. L., Le, X. C., & Li, X. F. (2011). DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes. Analytical chemistry, 83(10), 3640–3647. https://doi.org/10.1021/ac200575e
Aptamer: 20A1
Target: Group A Streptococcus (GAS)*
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGCAGAACGCACCCGCACACCTCCATCACTCGCATGCACCCCCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.6
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 1× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Primer regions removed (no primer version of 20a1p)
Application as quoted in the referenced paper: Diagnostic  and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."
Additional information: A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition
Reference (2011, Anal Chem ): Hamula, C. L., Le, X. C., & Li, X. F. (2011). DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes. Analytical chemistry, 83(10), 3640–3647. https://doi.org/10.1021/ac200575e
Aptamer: 20A1P
Target: Group A Streptococcus (GAS)*
Type of nucleic acid: ssDNA
Sequence: 5′TTCACGGTAGCACGCATAGGCAGAACGCACCCGCACACCTCCATCACTCGCATGCACCCCCATCTGACCTCTGTGCTGCT3′
Sequence length: 80
GC content: 0.6
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 1× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic  and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."
Additional information: A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition
Reference (2011, Anal Chem ): Hamula, C. L., Le, X. C., & Li, X. F. (2011). DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes. Analytical chemistry, 83(10), 3640–3647. https://doi.org/10.1021/ac200575e
Aptamer: 20A8
Target: Group A Streptococcus (GAS)*
Type of nucleic acid: ssDNA
Sequence: 5'CCCCACGAATCGTTACTCTGGTCCTCTATTTCTCCTCCCC3'
Sequence length: 40
GC content: 0.55
Affinity: Kd: 4 ± 1 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 1× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Primer regions removed (no primer version of 20a8p)
Application as quoted in the referenced paper: Diagnostic  and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."
Additional information: A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition
Reference (2011, Anal Chem ): Hamula, C. L., Le, X. C., & Li, X. F. (2011). DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes. Analytical chemistry, 83(10), 3640–3647. https://doi.org/10.1021/ac200575e
Aptamer: 20A8P
Target: Group A Streptococcus (GAS)*
Type of nucleic acid: ssDNA
Sequence: 5'AGCAGCACAGAGGTCAGATGCCCCACGAATCGTTACTCTGGTCCTCTATTTCTCCTCCCCCCTATGCGTGCTACCGTGAA3'
Sequence length: 80
GC content: 0.55
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 1× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic  and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."
Additional information: A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition
Reference (2011, Anal Chem ): Hamula, C. L., Le, X. C., & Li, X. F. (2011). DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes. Analytical chemistry, 83(10), 3640–3647. https://doi.org/10.1021/ac200575e
Aptamer: 20A9
Target: Group A Streptococcus (GAS)*
Type of nucleic acid: ssDNA
Sequence: 5'CACACGCTGAAGAAACTGAGGTCGTAGGTTTTCTTCGGG3'
Sequence length: 39
GC content: 0.5128205128205128
Affinity: Kd: 13 ± 1 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 1× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Primer region removed (no primer version of 20a9)
Application as quoted in the referenced paper: Diagnostic  and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."
Additional information: A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition
Reference (2011, Anal Chem ): Hamula, C. L., Le, X. C., & Li, X. F. (2011). DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes. Analytical chemistry, 83(10), 3640–3647. https://doi.org/10.1021/ac200575e
Aptamer: 20A9P
Target: Group A Streptococcus (GAS)*
Type of nucleic acid: ssDNA
Sequence: 5'AGCAGCACAGAGGTCAGATGCACACGCTGAAGAAACTGAGGTCGTAGGTTTTCTTCGGGCCTATGCGTGCTACCGTGAA3'
Sequence length: 79
GC content: 0.5316455696202531
Affinity: Kd: 9.1 ± 0.8nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 1× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic  and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."
Additional information: A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition
Reference (2011, Anal Chem ): Hamula, C. L., Le, X. C., & Li, X. F. (2011). DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes. Analytical chemistry, 83(10), 3640–3647. https://doi.org/10.1021/ac200575e
Aptamer: 20A12P
Target: Group A Streptococcus (GAS)*
Type of nucleic acid: ssDNA
Sequence: 5'TTCACGGTAGCACGCATAGGGCCCGACACTCGTCCACCCGATACCTCTCATGTGTCCCCATCTGACCTCTGTGCTGCT3'
Sequence length: 78
GC content: 0.5897435897435898
Affinity: Kd: 25 ± 3 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 1× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic  and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."
Additional information: A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition
Reference (2011, Anal Chem ): Hamula, C. L., Le, X. C., & Li, X. F. (2011). DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes. Analytical chemistry, 83(10), 3640–3647. https://doi.org/10.1021/ac200575e
Aptamer: 20A14P
Target: Group A Streptococcus (GAS)*
Type of nucleic acid: ssDNA
Sequence: 5'AGCAGCACAGAGGTCAGATGGGCATGGGGAAGAGAAAGCGGGATAACTTCGTTACCGGGCCCTATGCGTGCTACCGTGAA3'
Sequence length: 80
GC content: 0.5625
Affinity: Kd: 17 ± 1 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 1× BB; 50 mM Tris–HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic  and Detection: "This paper describes the selection of high affinity DNA aptamers binding to multiple M-types of the pathogenic species Streptococcus pyogenes (Group A Streptococcus or GAS). Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively), are particularly promising. These aptamers could potentially be used to improve the detection of GAS, a pathogen that is the causative agent of many infectious diseases, most notably strep throat."
Additional information: A total of 10^8 cells, containing an equal number of cells of each M-type: Streptococcus pyogenes clinical isolates corresponding to M-types M1, M2, M3, M4, M5, M6, M11, M12, M28, M41, M49, M59, M75, M77, M82, M83, M89, M91, M92, and M114. 5' fam addition
Reference (2011, Anal Chem ): Hamula, C. L., Le, X. C., & Li, X. F. (2011). DNA aptamers binding to multiple prevalent M-types of Streptococcus pyogenes. Analytical chemistry, 83(10), 3640–3647. https://doi.org/10.1021/ac200575e
Aptamer: A-1
Target: Human immunodeficiency virus (HIV)-1Bal gp120 protein
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5'GGGAGGACGAUGCGGAAUUGAGGGACCACGCGCUGCUUGUUGUGAUAAGCAGUUUGUCGGAUGGCAGACGACUCGCCCGA3'
Sequence length: 80
GC content: 0.5875
Affinity: Kd: 52 nM
Pool type: 5'-GGGAGGACGATGCGG-N50-CAGACGACTCGCCCGA-3'
Pool random region: 50
Binding buffer/conditions: Low-salt RNA binding buffer (10 mM HEPES pH 7.4, 50 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 2.7 mM KCl, 10 mM DTT, 0.01% BSA and a high-salt RNA binding buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 2.7 mM KCl, 10 mM DTT, 0.01% BSA)
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " The potent ability of small interfering (si)RNAs to inhibit the expression of complementary RNA transcripts is being exploited as a new class of therapeutics for a variety of diseases including HIV. We utilize the gp120 aptamer-siRNA chimeras for cell-type specific delivery of the siRNA into HIV-1 infected cells. This dual function chimera shows considerable potential for combining various nucleic acid therapeutic agents (aptamer and siRNA) in suppressing HIV-1 infection, making the aptamer-siRNA chimeras attractive therapeutic candidates for patients failing highly active antiretroviral therapy (HAART)."
Additional information: 2'F-modified: Typically, prepare 20 μL of reaction containing 1 μg of purified DNA template, 2 μL 10 x buffer, 2 μL dATP, 2 μL dGTP, 2 μL 2'-F-dCTP, 2 μL 2'-F-dUTP, 2 μL DTT and 2 μL T7 RNA polymerase at room temperature and then incubate at 37°C for 6 h.
Reference (2011, J Vis Exp ): Zhou, J., Li, H., Zhang, J., Piotr, S., & Rossi, J. (2011). Development of cell-type specific anti-HIV gp120 aptamers for siRNA delivery. Journal of visualized experiments : JoVE, (52), 2954. https://doi.org/10.3791/2954
Aptamer: #3
Target: Bisphenol A (BPA)
Type of nucleic acid: ssDNA
Sequence: 5′GGGCCGTTCGAACACGAGCATGCCGGTGGGTGGTCAGGTGGGATAGCGTTCCGCGTATGGCCCAGCGCATCACGGGTTCGCACCAGGACAGTACTCAGGTCATCCTAGG3′
Sequence length: 109
GC content: 0.6330275229357798
Affinity: Kd: 8.3 nM 
Pool type: 5′-GGGCCGTTCGAACACGAGCATG-N60-GGACAGTACTCAGGTCATCCTAG-3′
Pool random region: 60
Binding buffer/conditions: 25 mM Tris-HCl, 100 mM NaCl, 25 mM KCl, 10 mM MgCl2, and 5% DMSO, pH 8.0
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: 228 da
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection and Diagnostics: "We report highly specific ssDNA oligonucleotide aptamers and an aptamer-based sol–gel chip assay for sensitive and selective detection of small molecule pollutants. These results demonstrate that specific aptamers of small molecules can be used in many sensor applications, such as environmental monitorization and food safety."
Reference (2011, Oligonucleotides ): Jo, M., Ahn, J. Y., Lee, J., Lee, S., Hong, S. W., Yoo, J. W., Kang, J., Dua, P., Lee, D. K., Hong, S., & Kim, S. (2011). Development of single-stranded DNA aptamers for specific Bisphenol a detection. Oligonucleotides, 21(2), 85–91. https://doi.org/10.1089/oli.2010.0267
Aptamer: #3
Target: 6 fluorine atoms difference bisphenol A (6F BPA)
Type of nucleic acid: ssDNA
Sequence: 5′GGGCCGTTCGAACACGAGCATGCCGGTGGGTGGTCAGGTGGGATAGCGTTCCGCGTATGGCCCAGCGCATCACGGGTTCGCACCAGGACAGTACTCAGGTCATCCTAGG3′
Sequence length: 109
GC content: 0.6330275229357798
Affinity: Kd: 208 mM
Pool type: 5′-GGGCCGTTCGAACACGAGCATG-N60-GGACAGTACTCAGGTCATCCTAGG-3′
Pool random region: 60
Binding buffer/conditions: 25 mM Tris-HCl, 100 mM NaCl, 25 mM KCl, 10 mM MgCl2, and 5% DMSO, pH 8.0
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: 228 da
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection and Diagnostics: "We report highly specific ssDNA oligonucleotide aptamers and an aptamer-based sol–gel chip assay for sensitive and selective detection of small molecule pollutants. These results demonstrate that specific aptamers of small molecules can be used in many sensor applications, such as environmental monitorization and food safety."
Reference (2011, Oligonucleotides ): Jo, M., Ahn, J. Y., Lee, J., Lee, S., Hong, S. W., Yoo, J. W., Kang, J., Dua, P., Lee, D. K., Hong, S., & Kim, S. (2011). Development of single-stranded DNA aptamers for specific Bisphenol a detection. Oligonucleotides, 21(2), 85–91. https://doi.org/10.1089/oli.2010.0267
Aptamer: #3
Target: Bisphenol B (BPB)
Type of nucleic acid: ssDNA
Sequence: 5′GGGCCGTTCGAACACGAGCATGCCGGTGGGTGGTCAGGTGGGATAGCGTTCCGCGTATGGCCCAGCGCATCACGGGTTCGCACCAGGACAGTACTCAGGTCATCCTAGG3′
Sequence length: 109
GC content: 0.6330275229357798
Affinity: Kd: 139 mM
Pool type: 5′-GGGCCGTTCGAACACGAGCATG-N60-GGACAGTACTCAGGTCATCCTAGG-3′
Pool random region: 60
Binding buffer/conditions: 25 mM Tris-HCl, 100 mM NaCl, 25 mM KCl, 10 mM MgCl2, and 5% DMSO, pH 8.0
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: 228 da
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection and Diagnostics: "We report highly specific ssDNA oligonucleotide aptamers and an aptamer-based sol–gel chip assay for sensitive and selective detection of small molecule pollutants. These results demonstrate that specific aptamers of small molecules can be used in many sensor applications, such as environmental monitorization and food safety."
Reference (2011, Oligonucleotides ): Jo, M., Ahn, J. Y., Lee, J., Lee, S., Hong, S. W., Yoo, J. W., Kang, J., Dua, P., Lee, D. K., Hong, S., & Kim, S. (2011). Development of single-stranded DNA aptamers for specific Bisphenol a detection. Oligonucleotides, 21(2), 85–91. https://doi.org/10.1089/oli.2010.0267
Aptamer: #3
Target: 4,4′-Bisphenol (BP)
Type of nucleic acid: ssDNA
Sequence: 5′GGGCCGTTCGAACACGAGCATGCCGGTGGGTGGTCAGGTGGGATAGCGTTCCGCGTATGGCCCAGCGCATCACGGGTTCGCACCAGGACAGTACTCAGGTCATCCTAGG3′
Sequence length: 109
GC content: 0.6330275229357798
Affinity: Kd: 139 mM
Pool type: 5′-GGGCCGTTCGAACACGAGCATG-N60-GGACAGTACTCAGGTCATCCTAGG-3′
Pool random region: 60
Binding buffer/conditions: 25 mM Tris-HCl, 100 mM NaCl, 25 mM KCl, 10 mM MgCl2, and 5% DMSO, pH 8.0
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: 228 da
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection and Diagnostics: "We report highly specific ssDNA oligonucleotide aptamers and an aptamer-based sol–gel chip assay for sensitive and selective detection of small molecule pollutants. These results demonstrate that specific aptamers of small molecules can be used in many sensor applications, such as environmental monitorization and food safety."
Reference (2011, Oligonucleotides ): Jo, M., Ahn, J. Y., Lee, J., Lee, S., Hong, S. W., Yoo, J. W., Kang, J., Dua, P., Lee, D. K., Hong, S., & Kim, S. (2011). Development of single-stranded DNA aptamers for specific Bisphenol a detection. Oligonucleotides, 21(2), 85–91. https://doi.org/10.1089/oli.2010.0267