RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: RM14
Target: Recombinant ovine PrP (prion protein) of the susceptible-specific (V136R154Q171) or resistant-specific (A136R154R171) alleles
Type of nucleic acid: ssRNA
Sequence: 5'GAGGAUCCGCCGAGUAACAGCGUGCAGGUACUAGCUCCACCCCGAGGGGGCACAUCAUUUCUUGAGGCUCGAAUUCGCCC3'
Sequence length: 80
GC content: 0.6
Affinity: Kd: 20 nM
Pool type: 5-GAGGATCCGCCGAGTAACAG-N40-CTTGAGGCTCGAATTCGCCC-3
Pool random region: 40
Binding buffer/conditions: “BB”, 50 mM Tris, 125 mM NaCl, 5 mM MgCl2, pH 8
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Detection: "Identify RNA motifs that bind recombinant ovine PrP of the susceptible-specific or resistant-specific alleles. Since the main phenotype in prion diseases consists in alterations of PrP’s properties, uncertainties about PrP’s functions represent a major obstacle in our understanding of the pathological process and impedes any attempt towards prevention and therapeutics of prion diseases."
Reference (2006, Arch Virol ): Mercey, R., Lantier, I., Maurel, M. C., Grosclaude, J., Lantier, F., & Marc, D. (2006). Fast, reversible interaction of prion protein with RNA aptamers containing specific sequence patterns. Archives of virology, 151(11), 2197–2214. https://doi.org/10.1007/s00705-006-0790-3
Aptamer: SSAP1-4
Target: Recombinant human (rhu) cellular prlon protein (Prpc)
Type of nucleic acid: ssDNA
Sequence: 5'TTTGGTCCTTGTCTTATGTCCAGAATGCGCGGATCGTGATTTCTCCTACTGGGATAGGTGGATTAT3'
Sequence length: 66
GC content: 0.4545454545454545
Affinity: Not reported
Pool type: 5'-TTTGGTCCTTGTCTTATGTCCAGAATGC-N40 -ATTTCTCCTACTGGGATAGGTGGATTAT-3'
Pool random region: 40
Binding buffer/conditions: 1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) containing 0.05% Tween-20 (PBST)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "The Prpcspecific aptamers seem to recognize a conformationand could be used in competitive or double-ligand assayformats to differentiateprion isoforms, aiding in the diagnostics of TSEs. The Prpc-specific aptamers could also be applied as therapeutic tools to deter the progression of TSEs, and some aptamers developed in these studies mayfind application in the future to the decontamination of blood, body fluids, foods, pharmaceuticals, and cosmetics in an automated fashion during manufacture."
Reference (2006, Exp Biol Med (Maywood) ): Takemura, K., Wang, P., Vorberg, I., Surewicz, W., Priola, S. A., Kanthasamy, A., Pottathil, R., Chen, S. G., & Sreevatsan, S. (2006). DNA aptamers that bind to PrP(C) and not PrP(Sc) show sequence and structure specificity. Experimental biology and medicine (Maywood, N.J.), 231(2), 204–214. https://doi.org/10.1177/153537020623100211
Aptamer: SSAP3-10
Target: Recombinant human (rhu) cellular prlon protein (Prpc)
Type of nucleic acid: ssDNA
Sequence: 5'TTTGGTCCTTGTCTTATGTCCAGAATGCGTGGGGCAATTTCTCCTACTGGGATAGGTGGATTAT3'
Sequence length: 64
GC content: 0.453125
Affinity: Not reported
Pool type: 5'-TTTGGTCCTTGTCTTATGTCCAGAATGC-N40 -ATTTCTCCTACTGGGATAGGTGGATTAT-3'
Pool random region: 40
Binding buffer/conditions: 1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) containing 0.05% Tween-20 (PBST)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "The Prpcspecific aptamers seem to recognize a conformationand could be used in competitive or double-ligand assayformats to differentiateprion isoforms, aiding in the diagnostics of TSEs. The Prpc-specific aptamers could also be applied as therapeutic tools to deter the progression of TSEs, and some aptamers developed in these studies mayfind application in the future to the decontamination of blood, body fluids, foods, pharmaceuticals, and cosmetics in an automated fashion during manufacture."
Reference (2006, Exp Biol Med (Maywood) ): Takemura, K., Wang, P., Vorberg, I., Surewicz, W., Priola, S. A., Kanthasamy, A., Pottathil, R., Chen, S. G., & Sreevatsan, S. (2006). DNA aptamers that bind to PrP(C) and not PrP(Sc) show sequence and structure specificity. Experimental biology and medicine (Maywood, N.J.), 231(2), 204–214. https://doi.org/10.1177/153537020623100211
Aptamer: R9-2
Target: Caenorhabditis elegans Bcl-2 homolog CED-9
Type of nucleic acid: ssRNA
Sequence: 5'AGGGAGGACGAUGCGGGGUGCUUCGAGCGUAGGAAGAAAGCCGGGGGCUGCAGAUAAUGUAUAGCCAGACGACGGA3'
Sequence length: 76
GC content: 0.5921052631578947
Affinity: Kd: ~4nM
Pool type: 5'-AGGGAGGACGATGCG-N49-CAGACGACGGA-3'
Pool random region: 49
Binding buffer/conditions: 100 mM Tris-HCl (pH 8.0), 12.5 mM EDTA, 150 mM NaCl, and 1% SDS
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " Bcl-2 family proteins include anti- and proapoptotic factors that play important roles in regulating apoptosis in diverse species. Identification of compounds that can modulate the activities of Bcl-2 family proteins will facilitate development of drugs for treatment of apoptosis-related human diseases. We used an in vitro selection method named systematic evolution of ligands by exponential enrichment (SELEX) to isolate RNA aptamers that bind the Caenorhabditis elegans Bcl-2 homolog CED-9. Potent RNA aptamers specific for Bcl-2, Bcl-xL, or other Bcl-2 family proteins will be useful not only for studying how Bcl-2 family proteins regulate activation of apoptosis but also for developing new diagnostic reagents or therapeutic drugs to detect or treat those human diseases caused by abnormal apoptosis."
Additional information: T7 Promoter: AATACGACTCACTATAG. 32P-labeled RNA
Reference (2006, J Biol Chem ): Yang, C., Yan, N., Parish, J., Wang, X., Shi, Y., & Xue, D. (2006). RNA aptamers targeting the cell death inhibitor CED-9 induce cell killing in Caenorhabditis elegans. The Journal of biological chemistry, 281(14), 9137–9144. https://doi.org/10.1074/jbc.M511742200
Aptamer: R9-7
Target: Caenorhabditis elegans Bcl-2 homolog CED-9
Type of nucleic acid: ssRNA
Sequence: 5'AGGGAGGACGAUGCGGGAUGGACGCUUAUCCGCAUAGAGGUUUACUACUUCGGAGACUGCCGAUACAGACGACGGA3'
Sequence length: 76
GC content: 0.5526315789473685
Affinity: Kd: ~16 nM
Pool type: 5'-AGGGAGGACGATGCG-N49-CAGACGACGGA-3'
Pool random region: 49
Binding buffer/conditions: 100 mM Tris-HCl (pH 8.0), 12.5 mM EDTA, 150 mM NaCl, and 1% SDS
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " Bcl-2 family proteins include anti- and proapoptotic factors that play important roles in regulating apoptosis in diverse species. Identification of compounds that can modulate the activities of Bcl-2 family proteins will facilitate development of drugs for treatment of apoptosis-related human diseases. We used an in vitro selection method named systematic evolution of ligands by exponential enrichment (SELEX) to isolate RNA aptamers that bind the Caenorhabditis elegans Bcl-2 homolog CED-9. Potent RNA aptamers specific for Bcl-2, Bcl-xL, or other Bcl-2 family proteins will be useful not only for studying how Bcl-2 family proteins regulate activation of apoptosis but also for developing new diagnostic reagents or therapeutic drugs to detect or treat those human diseases caused by abnormal apoptosis."
Additional information: T7 Promoter: AATACGACTCACTATAG. 32P-labeled RNA
Reference (2006, J Biol Chem ): Yang, C., Yan, N., Parish, J., Wang, X., Shi, Y., & Xue, D. (2006). RNA aptamers targeting the cell death inhibitor CED-9 induce cell killing in Caenorhabditis elegans. The Journal of biological chemistry, 281(14), 9137–9144. https://doi.org/10.1074/jbc.M511742200
Aptamer: r10/43
Target: RNA-dependent RNA polymerase (RdRp) (nonstructural protein 5B [NS5B]) of HCV subtype 3a
Type of nucleic acid: ssDNA
Sequence: 5′GGGAGACAAGAATAAACGCTCAAGGGCGTGGTGGGTGGGGTACTAATAATGTGCGTTTGTTCGACAGGAGGCTCACAACAGGC3′
Sequence length: 83
GC content: 0.5301204819277109
Affinity: Kd: 1.3 ± 0.3 nM
Pool type: 5′-GGGAGACAAGAATAAACGCTCAA-N36-TTCGACAGGAGGCTCACAACAGGC-3′
Pool random region: 36
Binding buffer/conditions: 50 mM sodium phosphate, pH 8.0, 0.5 M NaCl, 10 mM imidazole, 10 mM β-mercaptoethanol, 10% glycerol, 0.2% n-octyl glucoside, 1:200 [vol/vol] protease inhibitor cocktail [Calbiochem, San Diego, California]
Buffer type: PBS/phosphate buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " Research into antiviral agents directed at hepatitis C virus (HCV) proteins is commonly based and tested on a single genotype, namely, genotype 1. Advances in therapeutic options for the treatment of HCV infection are of paramount importance. The SELEX approach was used in the present study to isolate DNA aptamers to the RNA-dependent RNA polymerase (RdRp) (nonstructural protein 5B [NS5B]) of HCV subtype 3a, with the aim of inhibiting polymerase activity. Inhibitory aptamers, directed at key enzymatic targets could be used as novel antiviral agents or useful tools in research."
Reference (2006, Antimicrob Agents Chemother ): Jones, L. A., Clancy, L. E., Rawlinson, W. D., & White, P. A. (2006). High-affinity aptamers to subtype 3a hepatitis C virus polymerase display genotypic specificity. Antimicrobial agents and chemotherapy, 50(9), 3019–3027. https://doi.org/10.1128/AAC.01603-05
Aptamer: r10/47
Target: RNA-dependent RNA polymerase (RdRp) (nonstructural protein 5B [NS5B]) of HCV subtype 3a
Type of nucleic acid: ssDNA
Sequence: 5′GGGAGACAAGAATAAACGCTCAATTGGGGTCTGCTCGGGATTGCGGAGAACGTGAATCTTTCGACAGGAGGCTCACAACAGGC3′
Sequence length: 83
GC content: 0.5301204819277109
Affinity: Kd: 23.5 ± 6.7 nM 
Pool type: 5′-GGGAGACAAGAATAAACGCTCAA-N36-TTCGACAGGAGGCTCACAACAGGC-3′
Pool random region: 36
Binding buffer/conditions: 50 mM sodium phosphate, pH 8.0, 0.5 M NaCl, 10 mM imidazole, 10 mM β-mercaptoethanol, 10% glycerol, 0.2% n-octyl glucoside, 1:200 [vol/vol] protease inhibitor cocktail [Calbiochem, San Diego, California]
Buffer type: PBS/phosphate buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " Research into antiviral agents directed at hepatitis C virus (HCV) proteins is commonly based and tested on a single genotype, namely, genotype 1. Advances in therapeutic options for the treatment of HCV infection are of paramount importance. The SELEX approach was used in the present study to isolate DNA aptamers to the RNA-dependent RNA polymerase (RdRp) (nonstructural protein 5B [NS5B]) of HCV subtype 3a, with the aim of inhibiting polymerase activity. Inhibitory aptamers, directed at key enzymatic targets could be used as novel antiviral agents or useful tools in research."
Reference (2006, Antimicrob Agents Chemother ): Jones, L. A., Clancy, L. E., Rawlinson, W. D., & White, P. A. (2006). High-affinity aptamers to subtype 3a hepatitis C virus polymerase display genotypic specificity. Antimicrobial agents and chemotherapy, 50(9), 3019–3027. https://doi.org/10.1128/AAC.01603-05
Aptamer: clone 5
Target: Bovine factor IX
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGCUCAGCCUUCACUGC-CUACGCGGGCGUUUACGUAACGGCUUAUGGGGAGCUGAGCGCUUGACCGUGGUAGUGCUAAGCAGUAAACGAG-GGCACCACGGUCGGAUCCAC3'
Sequence length: 113
GC content: 0.6017699115044248
Affinity: Kd: 10 nM
Pool type: 5'-TCGAGCTCAGCCTTCACTGC–N74-GGCACCACGGTCGGATCCAC-3'
Pool random region: 74
Binding buffer/conditions: 50 mM Tris-HCl, pH 7.5, 25 mM NaCl, 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " In the present investigation we have shown an RNA aptamer that dintinguishes bovine factor IX from human IX as a new member for discriminating aptamers. Developing these kinds of specific probes that distinguishes closely related species will have great impact in developing specific diagnostic reagents."
Additional information: The aptamer sequence was obtained from the fiigure 5. (there is potential for error since the quality of figure was not great)
Reference (2006, J Biochem ): Gopinath, S. C., Balasundaresan, D., Akitomi, J., & Mizuno, H. (2006). An RNA aptamer that discriminates bovine factor IX from human factor IX. Journal of biochemistry, 140(5), 667–676. https://doi.org/10.1093/jb/mvj203
Aptamer: Class A-20
Target: Influenza B virus hemagglutinin (HA) (B/Johannesburg/05/1999), Human
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGCUCAGCCUUCACUGCACUCCGGCUGGUGGACGCGGUACGAGCAAUUUGUACCGGAUGGAUGUUCGGGCAGCGGUGUGGCAGGGAUGAGCGGCACCACGGUCGGAUCCAC3'
Sequence length: 114
GC content: 0.6403508771929824
Affinity: Kd: 44 ± 6 nM
Pool type: 5'-TCGAGCTCAGCCTTCACTGC–N74-GGCACCACGGTCGGATCCAC-3'
Pool random region: 74
Binding buffer/conditions: 50 mM Tris-HCl, pH 7.5, 25 mM NaCl, 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " In the present investigation we have shown an RNA aptamer that dintinguishes bovine factor IX from human IX as a new member for discriminating aptamers. Developing these kinds of specific probes that distinguishes closely related species will have great impact in developing specific diagnostic reagents."
Additional information: requires 5 mM MgCl2 ion for its recognition
Reference (2006, J Biochem ): Gopinath, S. C., Sakamaki, Y., Kawasaki, K., & Kumar, P. K. (2006). An efficient RNA aptamer against human influenza B virus hemagglutinin. Journal of biochemistry, 139(5), 837–846. https://doi.org/10.1093/jb/mvj095
Aptamer: S10
Target: Cytoplasmic tail of BACE (B1-CT)
Type of nucleic acid: ssRNA
Sequence: 5′GGGAUAGGAUCCACAUCUACGUAUUAGUACACGUCGGCCACCUACGCGAAGUGGAAGCCUCAUUUGUUCACUGCAGACUUGACGAAGCUU3′
Sequence length: 90
GC content: 0.5
Affinity: Kd: 360 nM
Pool type: 5′-GGGATAGGATCCACATCTACGTATTA–N40-TTCACTGCAGACTTGACGAAGCTT-3′
Pool random region: 40
Binding buffer/conditions: PBS, 1 mM MgCl2 at pH 7.4
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " The β-amyloid peptide (Aβ) is a major component of the Alzheimer's disease (AD)-associated senile plaques and is generated by sequential cleavage of the β-amyloid precursor protein (APP) by β-secretase and γ-secretase. Since BACE1 initiates Aβ generation it represents a valuable target to interfere with Aβ production and treatment of AD. Because phosphorylation and GGA1 binding to B1-CT regulate BACE1 transport, these RNA inhibitors could be applied to investigate B1-CT activity without affecting the subcellular localization of BACE1."
Additional information: Doped pool for affinity maturation (M-selection)
Reference (2006, RNA ): Rentmeister, A., Bill, A., Wahle, T., Walter, J., & Famulok, M. (2006). RNA aptamers selectively modulate protein recruitment to the cytoplasmic domain of beta-secretase BACE1 in vitro. RNA (New York, N.Y.), 12(9), 1650–1660. https://doi.org/10.1261/rna.126306
Aptamer: P30-10-16
Target: Haemagglutinin (HA) region of the target strain A/Panama/2007/1999(H3N2)
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGAAUUCCGACCAGAAGGGUUAAGCAGUCGGCAUGCGGUACAGACAGACCUUUCCUCUCUCCUUCCUCUUCU3'
Sequence length: 75
GC content: 0.5333333333333333
Affinity: Kd: 188 pM
Pool type: 5′-GGGAGAATTCCGACCAGAAG-N30-CCTTTCCTCTCTCCTTCCTCTTCT-3′.
Pool random region: 30
Binding buffer/conditions: 20 nM HEPES/KOH (pH 7.4), 105 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection and Therapeutic: "It is important to determine whether a highly pathogenic form of HA exists in an individual strain in addition to typing and subtyping the viruses, especially in a diagnostic scenario where the severity of an infectious agent must be predicted. In this study, we demonstrated that the selected aptamer has the ability to distinguish the viruses and, more specifically, to distinguish strains within subtypes of influenza type A viruses."
Reference (2006, J Gen Virol ): Gopinath, S. C. B., Misono, T. S., Kawasaki, K., Mizuno, T., Imai, M., Odagiri, T., & Kumar, P. K. R. (2006). An RNA aptamer that distinguishes between closely related human influenza viruses and inhibits haemagglutinin-mediated membrane fusion. The Journal of general virology, 87(Pt 3), 479–487. https://doi.org/10.1099/vir.0.81508-0
Aptamer: A3
Target: Ricin toxin
Type of nucleic acid: ssDNA
Sequence: 5'ATAGGAGTCACGACGACCAGAACCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGGTGGCGTGGTATGTGCGTCTACCTCTTGACTAAT3'
Sequence length: 87
GC content: 0.5632183908045977
Affinity: Kd: 105 ± 41 nM
Pool type: 5'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'
Pool random region: 40
Binding buffer/conditions: 20 mM HEPES pH 7.35, 120 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1 mM MgCl2
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.3
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " The ricin toxin is easily and widely available, and also considered to be a potential agent of warfare or terrorist attack. Therefore, selection of aptamers which specifically recognize ricin and can be used as biocomponents 
in biosensors (aptasensors) should be paid more attention."
Reference (2006, Electrophoresis ): Tang, J., Xie, J., Shao, N., & Yan, Y. (2006). The DNA aptamers that specifically recognize ricin toxin are selected by two in vitro selection methods. Electrophoresis, 27(7), 1303–1311. https://doi.org/10.1002/elps.200500489
Aptamer: C1
Target: Ricin toxin
Type of nucleic acid: ssDNA
Sequence: 5'ATAGGAGTCACGACGACCAGAACCGTAGGTTCGGGGTCGGAGTGGTCCGGAAGATGGCGTGGTATGTGCGTCTACCTCTTGACTAAT3'
Sequence length: 87
GC content: 0.5517241379310345
Affinity: Kd: 80 ± 38 nM
Pool type: 5'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'
Pool random region: 40
Binding buffer/conditions: 20 mM HEPES pH 7.35, 120 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1 mM MgCl2
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.3
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " The ricin toxin is easily and widely available, and also considered to be a potential agent of warfare or terrorist attack. Therefore, selection of aptamers which specifically recognize ricin and can be used as biocomponents 
in biosensors (aptasensors) should be paid more attention."
Reference (2006, Electrophoresis ): Tang, J., Xie, J., Shao, N., & Yan, Y. (2006). The DNA aptamers that specifically recognize ricin toxin are selected by two in vitro selection methods. Electrophoresis, 27(7), 1303–1311. https://doi.org/10.1002/elps.200500489
Aptamer: C5
Target: Ricin toxin
Type of nucleic acid: ssDNA
Sequence: 5'ATAGGAGTCACGACGACCAGAACCGTAGGTTCGGGGCGGAGTGGTCCGGAAGGTGGCGTGGTATGTGCGTCTACCTCTTGACTAAT3'
Sequence length: 86
GC content: 0.5697674418604651
Affinity: Kd: 58 ± 19 nM
Pool type: 5'-ATAGGAGTCACGACGACCAGAA-N40-TATGTGCGTCTACCTCTTGACTAAT-3'
Pool random region: 40
Binding buffer/conditions: 20 mM HEPES pH 7.35, 120 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1 mM MgCl2
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.3
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " The ricin toxin is easily and widely available, and also considered to be a potential agent of warfare or terrorist attack. Therefore, selection of aptamers which specifically recognize ricin and can be used as biocomponents 
in biosensors (aptasensors) should be paid more attention."
Reference (2006, Electrophoresis ): Tang, J., Xie, J., Shao, N., & Yan, Y. (2006). The DNA aptamers that specifically recognize ricin toxin are selected by two in vitro selection methods. Electrophoresis, 27(7), 1303–1311. https://doi.org/10.1002/elps.200500489
Aptamer: PB9 aptamer
Target: Protein kinase C-delta (PKCδ)
Type of nucleic acid: ssDNA
Sequence: 5'GCCAGGGGTTCCACTACGTAGAACACGACGGGAATACTGACTCTCCCCCATGTACCAGGGGGCAGAGAGAAGGGC3'
Sequence length: 75
GC content: 0.6
Affinity: Kd: 122 nM
Pool type: 5'-GCCAGGGGTTCCACTACGTAGA-N30-ACCAGGGGGCAGAGAGAAGGGC-3'
Pool random region: 30
Binding buffer/conditions: Not reported
Buffer type: Not Reported
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " Selected high-affinity DNA aptamers for PKCδ by capillary electrophoresis based SELEX (systematic evolution of ligands by exponential enrichment, CE-SELEX). We have demonstrated that fluorescently tagged PB9 aptamer can specifically recognize PKCδ under in vitro conditions."
Additional information: Labeled clone pb9 with tmr (tetramethylrhodamine) at the 5′ end
Reference (2006, Chem Commun (Camb) ): Mallikaratchy, P., Stahelin, R. V., Cao, Z., Cho, W., & Tan, W. (2006). Selection of DNA ligands for protein kinase C-delta. Chemical communications (Cambridge, England), (30), 3229–3231. https://doi.org/10.1039/b604778e
Aptamer: A07
Target: Recombinant transforming growth factor-beta type III receptor displayed on cell surface
Type of nucleic acid: ssRNA
Sequence: 5'GGGCCAGGCAGCGAGAGAUAAGCAGAAGAAGUAUGUGACCAUGCUCCAGAGAGCAACUUCACAUGCGUAGCCAAACCGACCACACGCGUCCGAGA3'
Sequence length: 95
GC content: 0.5578947368421052
Affinity: Kd: 2.47 nM
Pool type: 5′-GGAGGACGTGCAGGGCCA-N60-CCACACGCGTCCGAGACAC-3′
Pool random region: 60
Binding buffer/conditions: 20 mM HEPES-NaOH, pH 7.4, 150 mM NaCl, 1.5 mM CaCl2, 0.5 mM MgCl2
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " When antibodies or aptamers are raised against the soluble extracellular fragments of target receptors in vitro, they are not always reactive to the native receptors on the cell surface. On the other hand, our novel TECS-SELEX method is able to overcome this problem and thus has additional promise to ensure fully reactive RNA aptamers against target receptors on living cells."
Additional information: Doped pool with random region doped at 30% of the following nucleotides: GGCAGCGAAAGCAGAAGAAGTATGTGACCATGCTTCGGCAACTTCACATGCGTAGCCAAACCGA

More about target: Chinese hamster ovary (CHO-K1) cells ectopically expressing human transforming growth factor-beta type III receptor (TβRIII)
Reference (2006, Biochimie ): Ohuchi, S. P., Ohtsu, T., & Nakamura, Y. (2006). Selection of RNA aptamers against recombinant transforming growth factor-β type III receptor displayed on cell surface. Biochimie, 88(7), 897–904. https://doi.org/10.1016/j.biochi.2006.02.004