RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: S-18
Target: Acetamiprid
Type of nucleic acid: ssDNA
Sequence: 5'TGTAATTTGTCTGCAGCGGTTCTTGATCGCTGACACCATATTATGAAGA3'
Sequence length: 49
GC content: 0.4081632653061224
Affinity: Kd: 4.98 μM
Pool type: 5'-CCTGCCACGCTCCGCAAGCTT-N10-CTGCAGCGATTCTTGATCG-N20-TAAGCTTGGCACCCGCATCGT-3'
Pool random region: 30
Binding buffer/conditions: Not reported
Buffer type: Not Reported
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Biosensor: " Our approach combines the inherent peroxidase-like nanozyme activity of gold nanoparticles (GNPs) with high affinity and specificity of an acetamiprid-specific S-18 aptamer to detect this neurotoxic pesticide in a highly rapid, specific, and sensitive manner. This approach allowed detection of 0.1 ppm acetamiprid within an assay time of 10 min. This reversible nanozyme activation/inhibition strategy may in principle be universally applicable for the detection of a range of environmental or biomedical molecules of interest."
Additional information: modified with 5'FAM fluorescent tag
Reference (2014, Anal Chem ): Weerathunge, P., Ramanathan, R., Shukla, R., Sharma, T. K., & Bansal, V. (2014). Aptamer-controlled reversible inhibition of gold nanozyme activity for pesticide sensing. Analytical chemistry, 86(24), 11937–11941. https://doi-org.ezproxy.lib.utexas.edu/10.1021/ac5028726
Aptamer: AGC03
Target: Gastric cancer cell-line (HGC-27)
Type of nucleic acid: ssDNA
Sequence: 5′ACGCTCGGATGCCACTACAGGGGGGTGGTCCTGAGGGTGGTGTGGTTGGTTTGGTTTCCTCATGGACGTGCTGGTGAC3'
Sequence length: 78
GC content: 0.6025641025641025
Affinity: Kd: 16.49 ± 0.40 nM
Pool type: 5′-ACGCTCGGATGCCACTACAG-N40-CTCATGGACGTGCTGGTGAC-3′
Pool random region: 40
Binding buffer/conditions: 4.5 g/l glucose, 5 mM MgCl2, 2 mg/ml BSA and 0.2 mg/ml yeast tRNA dissolved in PBS buffer
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Drug Delivery: " Gastric cancer is one of the most prevailing cancers with high morbidity and mortality. After 19 cycles of systematic evolution of ligands by exponential enrichment and subsequent cloning and sequencing, an aptamer with the highest affinity and specificity (nominated as AGC03) among candidates was screened out from a random single-stranded DNA pool. Our most important finding is that AGC03 could even be internalized into cells automatically. In conclusion, we obtained a novel aptamer specifically targeting gastric cancer,which is an effective tool for both gastric cancer diagnosis and drug delivery."
Reference (2014, Int J Biochem Cell Biol ): Zhang, X., Zhang, J., Ma, Y., Pei, X., Liu, Q., Lu, B., Jin, L., Wang, J., & Liu, J. (2014). A cell-based single-stranded DNA aptamer specifically targets gastric cancer. The international journal of biochemistry & cell biology, 46, 1–8.
Aptamer: 8-1
Target: Hemagglutinin (HA) of highly pathogenic avian influenza (HPAI H5N1)
Type of nucleic acid: ssRNA
Sequence: 5'UCUAAUACGACUCACUAUAGGAGCUCAGCCUUCACUGCCUGUUAGAGUUUCCUAAAAGCGAACUGGCGCCCUCGUCAGCAUCUGGCAGACGAGUGGAGACGGACUAACCACAGGCACCACGGUCGGAUCCUG3'
Sequence length: 132
GC content: 0.5454545454545454
Affinity: Not reported
Pool type: 5'-GGAGCTCAGCCTTCACTGC-N74-GGCA CCACGGTCGGATCCTG-3'
Pool random region: 74
Binding buffer/conditions: 10 mM HEPES, pH 7.4; 150 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " Highly pathogenic avian influenza (HPAI) H5 and H7 viruses have ravaged the poultry industry in numerous countries in Asia, Europe, Africa and the Middle East, and have resulted in the deaths of millions of birds. These studies suggest that aptamer 8-3S should be studied further to explore its potential applications not only in surveillance and diagnosis, but also in the development of H5N1- and H7N7-specific virucidal products that interfere with virus-host interactions to contain future H5N1 and H7N7 pandemics."
Additional information: Specificity of aptamers for the HAs of different subtypes of influenza A virus were measured and reported
Reference (2014, Acta Biomater ): Suenaga, E., & Kumar, P. K. (2014). An aptamer that binds efficiently to the hemagglutinins of highly pathogenic avian influenza viruses (H5N1 and H7N7) and inhibits hemagglutinin-glycan interactions. Acta biomaterialia, 10(3), 1314–1323. https://doi-org.ezproxy.lib.utexas.edu/10.1016/j.actbio.2013.12.034
Aptamer: 8-3
Target: Hemagglutinin (HA) of highly pathogenic avian influenza (HPAI H5N1)
Type of nucleic acid: ssRNA
Sequence: 5'UCUAAUACGACUCACUAUAGGAGCUCAGCCUUCACUGCGAGAUUGUUCUGACCGAGUUGCCUAGCAGGGCAACCGCUGGAACUUGAAGUCGGUAAUGCGAGCGGAAAGCCUUGGCACCACGGUCGGAUCCUG3'
Sequence length: 132
GC content: 0.5454545454545454
Affinity: Kd: 170 pM
Pool type: 5'-GGAGCTCAGCCTTCACTGC-N74-GGCA CCACGGTCGGATCCTG-3'
Pool random region: 74
Binding buffer/conditions: 10 mM HEPES, pH 7.4; 150 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " Highly pathogenic avian influenza (HPAI) H5 and H7 viruses have ravaged the poultry industry in numerous countries in Asia, Europe, Africa and the Middle East, and have resulted in the deaths of millions of birds. These studies suggest that aptamer 8-3S should be studied further to explore its potential applications not only in surveillance and diagnosis, but also in the development of H5N1- and H7N7-specific virucidal products that interfere with virus-host interactions to contain future H5N1 and H7N7 pandemics."
Additional information: Specificity of aptamers for the HAs of different subtypes of influenza A virus were measured and reported
Reference (2014, Acta Biomater ): Suenaga, E., & Kumar, P. K. (2014). An aptamer that binds efficiently to the hemagglutinins of highly pathogenic avian influenza viruses (H5N1 and H7N7) and inhibits hemagglutinin-glycan interactions. Acta biomaterialia, 10(3), 1314–1323. https://doi-org.ezproxy.lib.utexas.edu/10.1016/j.actbio.2013.12.034
Aptamer: 8-3S
Target: Hemagglutinin (HA) of highly pathogenic avian influenza (HPAI H5N1)
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5'GGGCAACCGCUGGAACUUGAAGUCGGUAAUGCGUGCGGAAAGCCC3'
Sequence length: 45
GC content: 0.6
Affinity: Kd: 110 pM
Pool type: 5'-GGAGCTCAGCCTTCACTGC-N74-GGCA CCACGGTCGGATCCTG-3'
Pool random region: 74
Binding buffer/conditions: 10 mM HEPES, pH 7.4; 150 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " Highly pathogenic avian influenza (HPAI) H5 and H7 viruses have ravaged the poultry industry in numerous countries in Asia, Europe, Africa and the Middle East, and have resulted in the deaths of millions of birds. These studies suggest that aptamer 8-3S should be studied further to explore its potential applications not only in surveillance and diagnosis, but also in the development of H5N1- and H7N7-specific virucidal products that interfere with virus-host interactions to contain future H5N1 and H7N7 pandemics."
Additional information: Shortened version of aptamer 8-3. Was able to retain full-length aptamer's affinity.the presence of 2′F UTP, 2′OH CTP, 2′OH ATP and 2′OH GTP to generate 2′-fluoro-U aptamer 8-3S, and in the presence of 2′OH UTP, 2′F CTP, 2′OH ATP and 2′OH GTP to generate 2′-fluoro-C aptamer 8-3S.2′-fluoro modifications to pyrimines to improve the stability of the aptamer in the presence of endoribonucleases
Reference (2014, Acta Biomater ): Suenaga, E., & Kumar, P. K. (2014). An aptamer that binds efficiently to the hemagglutinins of highly pathogenic avian influenza viruses (H5N1 and H7N7) and inhibits hemagglutinin-glycan interactions. Acta biomaterialia, 10(3), 1314–1323. https://doi-org.ezproxy.lib.utexas.edu/10.1016/j.actbio.2013.12.034
Aptamer: 8-10
Target: Hemagglutinin (HA) of highly pathogenic avian influenza (HPAI H5N1)
Type of nucleic acid: ssRNA
Sequence: 5'UCUAAUACGACUCACUAUAGGAGCUCAGCCUUCACUGCGGUGACCGGAGGAAUACGCGGACGGAGAAAGGGUUGGCCUCGUGGAUGGCGGAUACCCGUCCAGGGAUCUUCUAGGCACCACGGUCGGAUCCUG3'
Sequence length: 132
GC content: 0.5833333333333334
Affinity: Not reported
Pool type: 5'-GGAGCTCAGCCTTCACTGC-N74-GGCA CCACGGTCGGATCCTG-3'
Pool random region: 74
Binding buffer/conditions: 10 mM HEPES, pH 7.4; 150 mM NaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " Highly pathogenic avian influenza (HPAI) H5 and H7 viruses have ravaged the poultry industry in numerous countries in Asia, Europe, Africa and the Middle East, and have resulted in the deaths of millions of birds. These studies suggest that aptamer 8-3S should be studied further to explore its potential applications not only in surveillance and diagnosis, but also in the development of H5N1- and H7N7-specific virucidal products that interfere with virus-host interactions to contain future H5N1 and H7N7 pandemics."
Additional information: Specificity of aptamers for the HAs of different subtypes of influenza A virus were measured and reported
Reference (2014,Acta Biomater): Suenaga, E., & Kumar, P. K. (2014). An aptamer that binds efficiently to the hemagglutinins of highly pathogenic avian influenza viruses (H5N1 and H7N7) and inhibits hemagglutinin-glycan interactions. Acta biomaterialia, 10(3), 1314–1323. https://doi-org.ezproxy.lib.utexas.edu/10.1016/j.actbio.2013.12.034
Aptamer: Apt1
Target: Glygoprotein 2 (GP2), Mouse
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAAUUCCGACCAGAAGCAAGUAGUUGGCGCCUACUAUAGAAAUACGCCUUUCCUCUCUCCUUCCUCUUCU3'
Sequence length: 74
GC content: 0.4864864864864865
Affinity: Kd: 110 ± 2.6 nM
Pool type: 5'-GGGAGAAUUCCGACCAGAAG-N30-CCUUUC­CUCUCUCCUUCCUCUUCU-3'
Pool random region: 30
Binding buffer/conditions: Phosphate buffered saline (PBS)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Drug Delivery: " Microfold (M) cells are intestinal epithelial cells specialized for sampling and transport of luminal antigens to gut-associated lymphoid tissue for initiation of both mucosal and systemic immune responses. In this study, we used Systematic Evolution of Ligands by EXponential enrichment (SELEX) to isolate aptamers specific to murine GP2 (mGP2). this aptamer was able to bind to mGP2 expressed on the cell surface. These results suggest that this mGP2-specific aptamer could serve as a valuable tool for testing M-cell-targeted vaccine delivery in the murine model system."
Reference (2014, Cell Struct Funct ): Hanazato, M., Nakato, G., Nishikawa, F., Hase, K., Nishikawa, S., & Ohno, H. (2014). Selection of an aptamer against mouse GP2 by SELEX. Cell structure and function, 39(1), 23–29. https://doi-org.ezproxy.lib.utexas.edu/10.1247/csf.13019
Aptamer: HA12-16
Target: Glycosylated receptor-binding domain of the HA protein (gHA1)
Type of nucleic acid: ssRNA
Sequence: 5'GGGUUCACUGCAGACUUGACGAAGCUUGCUUGACGGAGAUCAAGGGCGAGUCGCAUACCAAGUUGAUGGGGAAUGGAUCCACAUCUACGAAUUC3'
Sequence length: 94
GC content: 0.5106382978723404
Affinity: Not reported
Pool type: 5'-GGGUUCACUGCAGACUUGACGAAGCUU-N40-AAUGGAUCCACAUCUACGAAUUC-3'
Pool random region: 40
Binding buffer/conditions: 20 mM sodium phosphate, pH 7.5, 500 mM NaCl, and 20 mM imidazole
Buffer type: PBS/phosphate buffers
pH: 7.5
Molecular weight of target: 46 kDa
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " The influenza virus surface glycoprotein hemagglutinin (HA) is responsible for viral attachment to sialic acid-containing host cell receptors and it facilitates the initial stage of viral infection. In the present study, we isolated an RNA aptamer specific to the glycosylated receptor-binding domain of the HA protein (gHA1). The HA12-16 RNA aptamer suppresses viral attachment to host cells by neutralizing the receptor-binding site of influenza virus HA. These results indicate that the isolated RNA aptamer can be developed as an antiviral reagent against influenza through appropriate therapeutic formulation."
Additional information: To the best of our knowledge, this aptamer includes the primer/static regions. However, one of the paper's figures showed the secondary structure for only the random region.
Reference (2014, PLoS One ): Kwon, H. M., Lee, K. H., Han, B. W., Han, M. R., Kim, D. H., & Kim, D. E. (2014). An RNA aptamer that specifically binds to the glycosylated hemagglutinin of avian influenza virus and suppresses viral infection in cells. PloS one, 9(5), e97574. https://doi.org/10.1371/journal.pone.0097574
Aptamer: Aptamer 15–1
Target: Cortisol
Type of nucleic acid: ssDNA
Sequence: 5'GAATGGATCCACATCCATGGATGGGCAATGCGGGGTGGAGAATGGTTGCCGCACTTCGGCTTCACTGCAGACTTGACGAAGCTTGACGAA3'
Sequence length: 90
GC content: 0.5444444444444444
Affinity: Kd: 16.1 ± 0.6 μM
Pool type: 5'-GAATGGATCCACATCCATGG-N40-TTCACTGCAGACTTGACGAAGCTTGACGAA-3'
Pool random region: 40
Binding buffer/conditions: 50 mM Tris (Promega, ≥99.9 %), 137 mM NaCl (Sigma >99.5 %), 5 mM MgCl2 (Fluka, ≥98 %), pH 7.4
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " The first-known aptamer for the stress biomarker cortisol was selected using a tunable stringency magnetic bead selection strategy. The structure-switching aptamer was applied to a gold nanoparticle assay in buffer and was shown to discriminate between cortisol and two other stress biomarkers, norepinephrine and epinephrine, and a structurally analogous biomarker of liver dysfunction, cholic acid. We believe this approach enhances aptamer selection and serves as proof-of-principle work toward development of point-of-care diagnostics for medical, combat, or bioterrorism targets."
Reference (2014, Anal Bioanal Chem ): Martin, J. A., Chávez, J. L., Chushak, Y., Chapleau, R. R., Hagen, J., & Kelley-Loughnane, N. (2014). Tunable stringency aptamer selection and gold nanoparticle assay for detection of cortisol. Analytical and bioanalytical chemistry, 406(19), 4637–4647. https://doi.org/10.1007/s00216-014-7883-8
Aptamer: B1-4
Target: Interferon-gamma (IFN-γ)
Type of nucleic acid: ssDNA
Sequence: 5′CCGCCCAAATCCCTAAGAGAAGACTGTAATGACATCAAACCAGACACACTACACACGCA3′
Sequence length: 59
GC content: 0.4745762711864407
Affinity: Kd: 74.5 nM
Pool type: 5′-TGCGTGTGTAGTGTGTCTG-N21-CTCTTAGGGATTTGGGCGG-3′
Pool random region: 21
Binding buffer/conditions: Phosphate buffered saline (PBS)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection, Therapeutic, and Diagnostic: " nterferon-gamma (IFN-γ) is a glycoprotein generated by lymphocytes that possesses anti-tumor, antiviral and immunomodulatory functions. IFN-γ assays are broadly employed in immunological research and clinical diagnostic tests. In this study, a novel 59-mer DNA aptamer (B1-4) was developed for assay of intracellular IFN-γ. The selected aptamer hence provides a novel approach for assaying intracellular IFN-γ generated by a group of lymphocytes, and may have application potential in both scientific research and clinical laboratory test."
Additional information: aptamer can bind to both natural and paraformaldehyde-fixed IFN-γ
Reference (2014, PLoS One ): Cao, B., Hu, Y., Duan, J., Ma, J., Xu, D., & Yang, X. D. (2014). Selection of a novel DNA aptamer for assay of intracellular interferon-gamma. PloS one, 9(5), e98214. https://doi.org/10.1371/journal.pone.0098214
Aptamer: Cd-4
Target: Cadmium (II)
Type of nucleic acid: ssDNA
Sequence: 5'ACCGACCGTGCTGGACTCTGGACTGTTGTGGTATTATTTTTGGTTGTGCAGTATGAGCGAGCGTTGCG3'
Sequence length: 68
GC content: 0.5147058823529411
Affinity: Kd: 34.5 nM
Pool type: 5′-ACCGACCGTGCTGGACTCT-N30-AGTATGAGCGAGCGTTGCG-3′
Pool random region: 30
Binding buffer/conditions: 50 mM HEPES, 50 mM NaCl, 5 mM KCl, 5 mM MgCl2, pH 7.2
Divalent salt: MgCl
Buffer type: Other Buffers
pH: 7.2
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " The demand for selection of aptamers against various small chemical molecules has substantially increased in recent years. Herein we chose Cd(II) as a model of a small molecule with less sites, and proposed a novel SELEX strategy of immobilizing ssDNA libraries rather than target molecules on a matrix, for selection of aptamers with high affinity to Cd(II). The selected Cd-4 aptamer will offer a new substitute for the detection of Cd(II) or other applications like recovery of cadmium from polluted samples."
Reference (2014, Analyst ): Wu, Y., Zhan, S., Wang, L., & Zhou, P. (2014). Selection of a DNA aptamer for cadmium detection based on cationic polymer mediated aggregation of gold nanoparticles. The Analyst, 139(6), 1550–1561. https://doi.org/10.1039/c3an02117c
Aptamer: 8A-44
Target: Interleukin-8 (IL-8)
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5'GGGGGCUUAUCAUUCCAUUUAGUGUUAUGAUAACCUUCCCAUCA3'
Sequence length: 44
GC content: 0.4090909090909091
Affinity: Kd: 92.2 μM
Pool type: 5'-GGGAGAGCGGAAGCGTGCTGGGCC-N40-CATAACCCAGAGGTCGATGGATCCCCCC-3
Pool random region: 40
Binding buffer/conditions: 30 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1.5 mM MgCl2, 2mM DTT, and 1% BSA
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Inhibition: " Interleukin-8 (IL-8) is a proinflammatory CXC chemokine that has been associated with the promotion of neutrophil chemotaxis, degranulation, and the pathogenesis of several neutrophil-infiltrating chronic inflammatory diseases. In the current study, we generated and characterized a 2'-fluoro-pyrimidine modified RNA aptamer (8A-35) against human IL-8. Our results suggest that the 8A-35 aptamer has great potential to be a lead structure in the development of effective therapeutic agents against inflammatory diseases."
Additional information: This aptamer was originally labelled as 8A-1F2R.
Reference (2014, Biomaterials ): Sung, H. J., Choi, S., Lee, J. W., Ok, C. Y., Bae, Y. S., Kim, Y. H., Lee, W., Heo, K., & Kim, I. H. (2014). Inhibition of human neutrophil activity by an RNA aptamer bound to interleukin-8. Biomaterials, 35(1), 578–589. https://doi.org/10.1016/j.biomaterials.2013.09.107
Aptamer: 8A-35
Target: Interleukin-8 (IL-8)
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5'GGGGGCUUAUCAUUCCAUUUAGUGUUAUGAUAACC3'
Sequence length: 35
GC content: 0.4
Affinity: Kd: 1.72 pM
Pool type: 5'-GGGAGAGCGGAAGCGTGCTGGGCC-N40-CATAACCCAGAGGTCGATGGATCCCCCC-3
Pool random region: 40
Binding buffer/conditions: 30 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1.5 mM MgCl2, 2mM DTT, and 1% BSA
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Truncated form of 8a-44; truncated to cut costs and filter out bases not essential for binding
Application as quoted in the referenced paper: Therapeutic and Inhibition: " Interleukin-8 (IL-8) is a proinflammatory CXC chemokine that has been associated with the promotion of neutrophil chemotaxis, degranulation, and the pathogenesis of several neutrophil-infiltrating chronic inflammatory diseases. In the current study, we generated and characterized a 2'-fluoro-pyrimidine modified RNA aptamer (8A-35) against human IL-8. Our results suggest that the 8A-35 aptamer has great potential to be a lead structure in the development of effective therapeutic agents against inflammatory diseases."
Reference (2014, Biomaterials ): Sung, H. J., Choi, S., Lee, J. W., Ok, C. Y., Bae, Y. S., Kim, Y. H., Lee, W., Heo, K., & Kim, I. H. (2014). Inhibition of human neutrophil activity by an RNA aptamer bound to interleukin-8. Biomaterials, 35(1), 578–589. https://doi.org/10.1016/j.biomaterials.2013.09.107
Aptamer: 8A-30
Target: Interleukin-8 (IL-8)
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5'GGGUUAUCAUUCCAUUUAGUGUUAUGAUAA3'
Sequence length: 30
GC content: 0.3
Affinity: Kd: 1.22 μM
Pool type: 5'-GGGAGAGCGGAAGCGTGCTGGGCC-N40-CATAACCCAGAGGTCGATGGATCCCCCC-3
Pool random region: 40
Binding buffer/conditions: 30 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1.5 mM MgCl2, 2mM DTT, and 1% BSA
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Truncated form of 8a-44; truncated to cut costs and filter out bases not essential for binding
Application as quoted in the referenced paper: Therapeutic and Inhibition: " Interleukin-8 (IL-8) is a proinflammatory CXC chemokine that has been associated with the promotion of neutrophil chemotaxis, degranulation, and the pathogenesis of several neutrophil-infiltrating chronic inflammatory diseases. In the current study, we generated and characterized a 2'-fluoro-pyrimidine modified RNA aptamer (8A-35) against human IL-8. Our results suggest that the 8A-35 aptamer has great potential to be a lead structure in the development of effective therapeutic agents against inflammatory diseases."
Reference (2014, Biomaterials ): Sung, H. J., Choi, S., Lee, J. W., Ok, C. Y., Bae, Y. S., Kim, Y. H., Lee, W., Heo, K., & Kim, I. H. (2014). Inhibition of human neutrophil activity by an RNA aptamer bound to interleukin-8. Biomaterials, 35(1), 578–589. https://doi.org/10.1016/j.biomaterials.2013.09.107
Aptamer: M49
Target: Murine CD200R1
Type of nucleic acid: ssDNA
Sequence: 5'GACGATAGCGGTGACGGCACAGACGGACGTGACATGCTTGACCAACTCGCCGTATGCCGCTTCCGTCCGTCGCTC3'
Sequence length: 75
GC content: 0.6266666666666667
Affinity: Kd: 390 ± 86 nM
Pool type: 5'-GACGATAGCGGTGACGGCACAGACG-N25-CGTATGCCGCTTCCGTCCGTCGCTC-3'
Pool random region: 25
Binding buffer/conditions: PBS (pH 7.4)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapuetic: " CD200R1 expressed on the surface of myeloid and lymphoid cells delivers immune inhibitory signals to modulate inflammation when engaged with its ligand CD200. We report the development and characterization of DNA aptamers, which bind to murine CD200R1 and act as potent signalling molecules in the absence of exogenous CD200. As DNA aptamers exhibit inherent advantages over conventional protein-based therapeutics including low immunogenicity, ease of synthesis, low cost, and long shelf life, such CD200R1 agonistic aptamers may emerge as useful and safe nonsteroidal anti-inflammatory therapeutic agents."
Reference (2014, Mol Ther Nucleic Acids ): Prodeus, A., Cydzik, M., Abdul-Wahid, A., Huang, E., Khatri, I., Gorczynski, R., & Gariépy, J. (2014). Agonistic CD200R1 DNA Aptamers Are Potent Immunosuppressants That Prolong Allogeneic Skin Graft Survival. Molecular therapy. Nucleic acids, 3(8), e190. https://doi.org/10.1038/mtna.2014.41