RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: aptamer 2
Target: Anti-MUC1 IgG3 monoclonal antibody; anti-MUC1 IgG3 MAb, C595 MAb (epitope: Arg-Pro-Ala-Pro)
Type of nucleic acid: ssDNA
Sequence: 5′GGGAGACAAGAATAAACGCTCAAGAACTGAGGTGCTTTCCCAAAACCTTTCGACAGGAGGCTCACAACAGGC5′
Sequence length: 72
GC content: 0.5
Affinity: Kd: 666.67 nM
Pool type: 5′-GGGAGACAAGAATAAACGCTCAA-N25-TTCGACAGGAGGCTCACAACAGGC-′3.
Pool random region: 25
Binding buffer/conditions: Carbonate buffer (pH 9.6) or PBS (pH 7.4)
Buffer type: PBS/phosphate buffers
pH: 9.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "The ease of selection of aptamers against the C595 antibody and its binding characteristics make it possible for aptamers to be rapidly generated using the proposed selection methodology and used as antibody-targeting entities that could be used in a number of applications, ranging from antibody purification to immunoassays and vaccine applications. Finally, their labelling potential may allow their use in imaging and as anti-idiotypic targeted radiopharmaceuticals, with potentially numerous therapeutic applications in disease diagnosis, imaging and therapy."
Reference (2004, J Immunol Methods ): Missailidis, S., Thomaidou, D., Borbas, K. E., & Price, M. R. (2005). Selection of aptamers with high affinity and high specificity against C595, an anti-MUC1 IgG3 monoclonal antibody, for antibody targeting. Journal of immunological methods, 296(1-2), 45–62. https://doi.org/10.1016/j.jim.2004.10.011
Aptamer: K61
Target: ADP-ribosylation factors (ARF) nucleotide-binding site opener (ARNO)/cytohesin-2
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAGACAAGCUUGGGUCGCGUUUUGCUAUUGAUUUCUUUUAACCUGGCCUGUUUGGGCCUCUUGCUCUUCCUAGGAGU3'
Sequence length: 81
GC content: 0.4938271604938271
Affinity: Kd: 115 ± 3 nM
Pool type: 5′-TCGGAGAGACAAGCTTGGGTC-N40-CTCTTGCTCTTCCTAGGAGT-3′
Pool random region: 40
Binding buffer/conditions: 4.3 mM Na2HPO4/1.4 mM KH2PO4/2.7 mM KCl/147 mM NaCl/3.0 mM MgCl2, pH 7.4
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Drug Development: "Cytohesins are a family of highly homologous guanine nucleotide exchange factors (GEFs) that act on ADP-ribosylation factors (ARFs). Here, we selected and applied a specific inhibitor for ARF nucleotide-binding site opener (ARNO)/cytohesin-2, an RNA aptamer that clearly discriminates between cytohesin-1 and cytohesin-2. Our results indicate that intramer technology can be used not only for assigning novel biological functions to proteins or protein domains but also to prove nonredundancy of highly homologous proteins."
Additional information: In vitro transcription with T7 RNA polymerase yielded the corresponding RNA library
Reference (2004, Proc Natl Acad Sci U S A ): Theis, M. G., Knorre, A., Kellersch, B., Moelleken, J., Wieland, F., Kolanus, W., & Famulok, M. (2004). Discriminatory aptamer reveals serum response element transcription regulated by cytohesin-2. Proceedings of the National Academy of Sciences of the United States of America, 101(31), 11221–11226. https://doi.org/10.1073/pnas.0402901101
Aptamer: Aptamer 1
Target: Fibronectin
Type of nucleic acid: ssDNA
Sequence: 5'ATAGGAGTCGACCGACCAGAACATGCGCCTTCCCCCTGTGGTTGGTGTCAGTCGGCCTGTGTATGTGCGTCTACATCTAGACTCAT3'
Sequence length: 86
GC content: 0.5465116279069767
Affinity: Not reported
Pool type: 5'-ATAGGAGTCGACCGACCAGAA-N40-TATGTGCGTCTACATCTAGACTCAT-3'
Pool random region: 40
Binding buffer/conditions: PBS(+) buffer (pH 7.2) containing 150 mM NaCl, 2 mM MgCl2, and 2 mM CaCl2
Divalent salt: MgCl/CaCl
Buffer type: PBS/phosphate buffers
pH: 7.2
Molecular weight of target: 460 kDa
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " In cases where the interaction-responsible particular residues (epitopes) on the protein surface are identified, aptamers may be selected against an epitope-containing small peptide as target. The fibronectin-integrin interactions are responsible for such diverse cell activities as adhesion, morphology, signaling, growth, differentiation and multiplication, migration and translocation, and angiogenesis and transfer of tumor cells. We report here that the aptamers selected indeed inhibit cell adhesion."
Additional information: Selection occured in two steps: binding to a peptide affinity column to create an enriched library, followed by binding to a fibronectin coated plate
Reference (2004, Bioorg Med Chem Lett ): Ogawa, A., Tomita, N., Kikuchi, N., Sando, S., & Aoyama, Y. (2004). Aptamer selection for the inhibition of cell adhesion with fibronectin as target. Bioorganic & medicinal chemistry letters, 14(15), 4001–4004. https://doi.org/10.1016/j.bmcl.2004.05.042
Aptamer: Aptamer 2
Target: Fibronectin
Type of nucleic acid: ssDNA
Sequence: 5'ATAGGAGTCGACCGACCAGAACATGCGCCTTCCCCCTGTGGTTGGTGTCAGCCGGCCTGTGTATGTGCGTCTACATCTAGACTCAT3'
Sequence length: 86
GC content: 0.5581395348837209
Affinity: Not reported
Pool type: 5'-ATAGGAGTCGACCGACCAGAA-N40-TATGTGCGTCTACATCTAGACTCAT-3'
Pool random region: 40
Binding buffer/conditions: PBS(+) buffer (pH 7.2) containing 150 mM NaCl, 2 mM MgCl2, and 2 mM CaCl2
Divalent salt: MgCl/CaCl
Buffer type: PBS/phosphate buffers
pH: 7.2
Molecular weight of target: 460 kDa
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " In cases where the interaction-responsible particular residues (epitopes) on the protein surface are identified, aptamers may be selected against an epitope-containing small peptide as target. The fibronectin-integrin interactions are responsible for such diverse cell activities as adhesion, morphology, signaling, growth, differentiation and multiplication, migration and translocation, and angiogenesis and transfer of tumor cells. We report here that the aptamers selected indeed inhibit cell adhesion."
Additional information: Selection occured in two steps: binding to a peptide affinity column to create an enriched library, followed by binding to a fibronectin coated plate
Reference (2004, Bioorg Med Chem Lett ): Ogawa, A., Tomita, N., Kikuchi, N., Sando, S., & Aoyama, Y. (2004). Aptamer selection for the inhibition of cell adhesion with fibronectin as target. Bioorganic & medicinal chemistry letters, 14(15), 4001–4004. https://doi.org/10.1016/j.bmcl.2004.05.042
Aptamer: Clone 2.11
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGGTTACTGCGGGGTATGGGGACTGGTTGCGTGGCTTGGTGTCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.575
Affinity: Kd: 84 ± 37 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'
Pool random region: 40
Binding buffer/conditions: 8.1 mM Na2HPO4, 1.1 mM KH2PO4, 1 mM MgCl2, 2.7 mM KCl, and 40 mM NaCl at a pH of 8.0
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Research: " This study demonstrates the feasibility of applying CE−SELEX for small-molecule targets which contain a class of important compounds for cell signaling and regulation. The characteristic of nucleic acids of being able to be amplified by PCR makes even partial separation adequate for CE−SELEX to be carried out. It broadens the scope of targets for CE−SELEX and also provides a new technique for selection of aptamers toward small-molecule targets."
Additional information: Electrophoretic selection with capillary electrophoresis (CE) is used
Reference (2004, J Am Chem Soc ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro evolution of functional DNA using capillary electrophoresis. Journal of the American Chemical Society, 126(1), 20–21. https://doi.org/10.1021/ja037832s
Aptamer: Clone 2.15
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGAAGCGATTGTAATCAACGCCGTAAGACCTCGAGTCGATTTCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.5
Affinity: Kd: 44 ± 9 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'
Pool random region: 40
Binding buffer/conditions: 8.1 mM Na2HPO4, 1.1 mM KH2PO4, 1 mM MgCl2, 2.7 mM KCl, and 40 mM NaCl at a pH of 8.0
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Research: " This study demonstrates the feasibility of applying CE−SELEX for small-molecule targets which contain a class of important compounds for cell signaling and regulation. The characteristic of nucleic acids of being able to be amplified by PCR makes even partial separation adequate for CE−SELEX to be carried out. It broadens the scope of targets for CE−SELEX and also provides a new technique for selection of aptamers toward small-molecule targets."
Additional information: Electrophoretic selection with capillary electrophoresis (CE) is used
Reference (2004, J Am Chem Soc ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro evolution of functional DNA using capillary electrophoresis. Journal of the American Chemical Society, 126(1), 20–21. https://doi.org/10.1021/ja037832s
Aptamer: Clone 2.22
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGCGCGCAGAATTTTGAGTCATGTACTAAGGAATTGATTGGTCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.475
Affinity: Kd: 37 ± 43 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'
Pool random region: 40
Binding buffer/conditions: 8.1 mM Na2HPO4, 1.1 mM KH2PO4, 1 mM MgCl2, 2.7 mM KCl, and 40 mM NaCl at a pH of 8.0
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Research: " This study demonstrates the feasibility of applying CE−SELEX for small-molecule targets which contain a class of important compounds for cell signaling and regulation. The characteristic of nucleic acids of being able to be amplified by PCR makes even partial separation adequate for CE−SELEX to be carried out. It broadens the scope of targets for CE−SELEX and also provides a new technique for selection of aptamers toward small-molecule targets."
Additional information: Electrophoretic selection with capillary electrophoresis (CE) is used
Reference (2004, J Am Chem Soc ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro evolution of functional DNA using capillary electrophoresis. Journal of the American Chemical Society, 126(1), 20–21. https://doi.org/10.1021/ja037832s
Aptamer: Clone 4.6
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGGTTCGTTGTAGCGCATAAAGTTTATCTCTCCCATGATTCACCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.475
Affinity: Kd: 27 ± 8 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'
Pool random region: 40
Binding buffer/conditions: 8.1 mM Na2HPO4, 1.1 mM KH2PO4, 1 mM MgCl2, 2.7 mM KCl, and 40 mM NaCl at a pH of 8.0
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Research: " This study demonstrates the feasibility of applying CE−SELEX for small-molecule targets which contain a class of important compounds for cell signaling and regulation. The characteristic of nucleic acids of being able to be amplified by PCR makes even partial separation adequate for CE−SELEX to be carried out. It broadens the scope of targets for CE−SELEX and also provides a new technique for selection of aptamers toward small-molecule targets."
Additional information: Electrophoretic selection with capillary electrophoresis (CE) is used
Reference (2004, J Am Chem Soc ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro evolution of functional DNA using capillary electrophoresis. Journal of the American Chemical Society, 126(1), 20–21. https://doi.org/10.1021/ja037832s
Aptamer: Clone 4.10
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGGGTAGGTAGCCGTGCCGGTTGTGCCATTGATTGTACAGTTCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.5375
Affinity: Kd: 73 ± 29 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'
Pool random region: 40
Binding buffer/conditions: 8.1 mM Na2HPO4, 1.1 mM KH2PO4, 1 mM MgCl2, 2.7 mM KCl, and 40 mM NaCl at a pH of 8.0
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Research: " This study demonstrates the feasibility of applying CE−SELEX for small-molecule targets which contain a class of important compounds for cell signaling and regulation. The characteristic of nucleic acids of being able to be amplified by PCR makes even partial separation adequate for CE−SELEX to be carried out. It broadens the scope of targets for CE−SELEX and also provides a new technique for selection of aptamers toward small-molecule targets."
Additional information: Electrophoretic selection with capillary electrophoresis (CE) is used
Reference (2004, J Am Chem Soc ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro evolution of functional DNA using capillary electrophoresis. Journal of the American Chemical Society, 126(1), 20–21. https://doi.org/10.1021/ja037832s
Aptamer: Clone 4.15
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGGCCTATATTTATAAAGACAGGAGAGTAATGTCGAGCAGAACCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.4625
Affinity: Kd: 33 ± 10 nM	
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'
Pool random region: 40
Binding buffer/conditions: 8.1 mM Na2HPO4, 1.1 mM KH2PO4, 1 mM MgCl2, 2.7 mM KCl, and 40 mM NaCl at a pH of 8.0
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Research: " This study demonstrates the feasibility of applying CE−SELEX for small-molecule targets which contain a class of important compounds for cell signaling and regulation. The characteristic of nucleic acids of being able to be amplified by PCR makes even partial separation adequate for CE−SELEX to be carried out. It broadens the scope of targets for CE−SELEX and also provides a new technique for selection of aptamers toward small-molecule targets."
Additional information: Electrophoretic selection with capillary electrophoresis (CE) is used
Reference (2004, J Am Chem Soc ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro evolution of functional DNA using capillary electrophoresis. Journal of the American Chemical Society, 126(1), 20–21. https://doi.org/10.1021/ja037832s
Aptamer: Apt-67
Target: Liver X receptors alpha (LXRalpha, LXRα)
Type of nucleic acid: ssDNA
Sequence: 5′ATGACCATGACCCTCCACACATGACGATGGGATCAGACTGTGGCAGGGAAAC3′
Sequence length: 52
GC content: 0.5384615384615384
Affinity: Kd: 18.4 pM
Pool type: 5′-ATGACCATGACCCTCCACAC-N12-TCAGACTGTGGCAGGGAAAC-3′
Pool random region: 12
Binding buffer/conditions: 20 mM Tris, 5 mM MgCl2, and 150 mM NaCl at pH 7.5
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Selection and use of small ssDNAs as alternative binders for HDL proteins. An essential function of HDL is linked with reverse cholesterol transport (RCT). Two sterol-responsive transcription factors, LXRa and LXRb, are directly involved in RCT. Crossreactivity studies show that the aptamer sequences, analyzed in this paper, would be more suitable as binders for LXRb"
Reference (2005, Biochem Biophys Res Commun ): Surugiu-Wärnmark, I., Wärnmark, A., Toresson, G., Gustafsson, J. A., & Bülow, L. (2005). Selection of DNA aptamers against rat liver X receptors. Biochemical and biophysical research communications, 332(2), 512–517. https://doi.org/10.1016/j.bbrc.2005.04.147
Aptamer: Apt-67
Target: Liver X receptors beta (LXRbeta, LXRβ)
Type of nucleic acid: ssDNA
Sequence: 5′ATGACCATGACCCTCCACACATGACGATGGGATCAGACTGTGGCAGGGAAAC3′
Sequence length: 52
GC content: 0.5384615384615384
Affinity: Kd: 8.02 pM
Pool type: 5′-ATGACCATGACCCTCCACAC-N12-TCAGACTGTGGCAGGGAAAC-3′
Pool random region: 12
Binding buffer/conditions: 22 mM Tris, 5 mM MgCl2, and 150 mM NaCl at pH 7.5
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Selection and use of small ssDNAs as alternative binders for HDL proteins. An essential function of HDL is linked with reverse cholesterol transport (RCT). Two sterol-responsive transcription factors, LXRa and LXRb, are directly involved in RCT. Crossreactivity studies show that the aptamer sequences, analyzed in this paper, would be more suitable as binders for LXRb"
Reference (2005, Biochem Biophys Res Commun ): Surugiu-Wärnmark, I., Wärnmark, A., Toresson, G., Gustafsson, J. A., & Bülow, L. (2005). Selection of DNA aptamers against rat liver X receptors. Biochemical and biophysical research communications, 332(2), 512–517. https://doi.org/10.1016/j.bbrc.2005.04.147
Aptamer: Apt-122
Target: Liver X receptors alpha (LXRalpha, LXRα)
Type of nucleic acid: ssDNA
Sequence: 5′ATGACCATGACCCTCCACACAATGCGCCGTCATCAGACTGTGGCAGGGAAAC3′
Sequence length: 52
GC content: 0.5576923076923077
Affinity: Kd: 24.2 pM
Pool type: 5′-ATGACCATGACCCTCCACAC-N12-TCAGACTGTGGCAGGGAAAC-3′
Pool random region: 12
Binding buffer/conditions: 20 mM Tris, 5 mM MgCl2, and 150 mM NaCl at pH 7.5
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Selection and use of small ssDNAs as alternative binders for HDL proteins. An essential function of HDL is linked with reverse cholesterol transport (RCT). Two sterol-responsive transcription factors, LXRa and LXRb, are directly involved in RCT. Crossreactivity studies show that the aptamer sequences, analyzed in this paper, would be more suitable as binders for LXRb"
Reference (2005, Biochem Biophys Res Commun ): Surugiu-Wärnmark, I., Wärnmark, A., Toresson, G., Gustafsson, J. A., & Bülow, L. (2005). Selection of DNA aptamers against rat liver X receptors. Biochemical and biophysical research communications, 332(2), 512–517. https://doi.org/10.1016/j.bbrc.2005.04.147
Aptamer: Apt-122
Target: Liver X receptors beta (LXRbeta, LXRβ)
Type of nucleic acid: ssDNA
Sequence: 5′ATGACCATGACCCTCCACACAATGCGCCGTCATCAGACTGTGGCAGGGAAAC3′
Sequence length: 52
GC content: 0.5576923076923077
Affinity: Kd: 7.9 pM
Pool type: 5′-ATGACCATGACCCTCCACAC-N12-TCAGACTGTGGCAGGGAAAC-3′
Pool random region: 12
Binding buffer/conditions: 23 mM Tris, 5 mM MgCl2, and 150 mM NaCl at pH 7.5
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Selection and use of small ssDNAs as alternative binders for HDL proteins. An essential function of HDL is linked with reverse cholesterol transport (RCT). Two sterol-responsive transcription factors, LXRa and LXRb, are directly involved in RCT. Crossreactivity studies show that the aptamer sequences, analyzed in this paper, would be more suitable as binders for LXRb"
Reference (2005, Biochem Biophys Res Commun ): Surugiu-Wärnmark, I., Wärnmark, A., Toresson, G., Gustafsson, J. A., & Bülow, L. (2005). Selection of DNA aptamers against rat liver X receptors. Biochemical and biophysical research communications, 332(2), 512–517. https://doi.org/10.1016/j.bbrc.2005.04.147
Aptamer: Apt- 14.3
Target: Ethanolamine
Type of nucleic acid: ssDNA
Sequence: 5′ATACCAGCTTATTCAATTTGAGGCGGGTGGGTGGGTTGAATATGCTGATTACCCCATCGGAGAACGTTAAGGCGCTTCAGATAGTAAGTGCAATCT3′
Sequence length: 96
GC content: 0.4583333333333333
Affinity: Kd: 6 ± 3 nM
Pool type: 5′-ATACCAGCTTATTCAATT-N60-AGATAGTAAGTGCAATCT-3′
Pool random region: 60
Binding buffer/conditions: 100 mmol L−1 NaCl/20 mmol L−1 Tris/HCl, pH 7.6/2 mmol L−1 MgCl2/5 mmol L−1 KCl/1 mmol L−1 CaCl2/0.02% Tween 20
Divalent salt: MgCl/CaCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " The aim of the development of ethanolamine aptamers is their use for the detection of this substance in clinical and environmental analysis. Ethanolamine is associated with several diseases. Moreover, ethanolamine and its derivatives di- and tri-ethanolamine are used in chemical and cosmetic industries. The use of biosensors with ethanolamine aptamer as new molecular recognition element could be an innovative method for an easy and fast detection of ethanolamine"
Reference (2005, Biochem Biophys Res Commun ): Mann, D., Reinemann, C., Stoltenburg, R., & Strehlitz, B. (2005). In vitro selection of DNA aptamers binding ethanolamine. Biochemical and biophysical research communications, 338(4), 1928–1934. https://doi.org/10.1016/j.bbrc.2005.10.172
Aptamer: Apt- 2.3
Target: Ethanolamine
Type of nucleic acid: ssDNA
Sequence: 5′ATACCAGCTTATTCAATTGCTGCGAGGTGGGTGGGTGGGAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCTAGATAGTAAGTGCAATCT3′
Sequence length: 96
GC content: 0.4895833333333333
Affinity: Kd: 17 ± 4 nM
Pool type: 5′-ATACCAGCTTATTCAATT-N60-AGATAGTAAGTGCAATCT-3′
Pool random region: 60
Binding buffer/conditions: 100 mmol L−1 NaCl/20 mmol L−1 Tris/HCl, pH 7.6/2 mmol L−1 MgCl2/5 mmol L−1 KCl/1 mmol L−1 CaCl2/0.02% Tween 20
Divalent salt: MgCl/CaCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " The aim of the development of ethanolamine aptamers is their use for the detection of this substance in clinical and environmental analysis. Ethanolamine is associated with several diseases. Moreover, ethanolamine and its derivatives di- and tri-ethanolamine are used in chemical and cosmetic industries. The use of biosensors with ethanolamine aptamer as new molecular recognition element could be an innovative method for an easy and fast detection of ethanolamine"
Reference (2005, Biochem Biophys Res Commun ): Mann, D., Reinemann, C., Stoltenburg, R., & Strehlitz, B. (2005). In vitro selection of DNA aptamers binding ethanolamine. Biochemical and biophysical research communications, 338(4), 1928–1934. https://doi.org/10.1016/j.bbrc.2005.10.172
Aptamer: Apt- 9.6
Target: Ethanolamine
Type of nucleic acid: ssDNA
Sequence: 5′ATACCAGCTTATTCAATTGCTGTGAGGTGGGTGGGTGGGAGCAATTGGTCCTCGCTTAGCTTCTACGGTGGGCTATCTAGATAGTAAGTGCAATCT3′
Sequence length: 96
GC content: 0.4791666666666667
Affinity: Kd: 15 ± 4 nM
Pool type: 5′-ATACCAGCTTATTCAATT-N60-AGATAGTAAGTGCAATCT-3′
Pool random region: 60
Binding buffer/conditions: 100 mmol L−1 NaCl/20 mmol L−1 Tris/HCl, pH 7.6/2 mmol L−1 MgCl2/5 mmol L−1 KCl/1 mmol L−1 CaCl2/0.02% Tween 20
Divalent salt: MgCl/CaCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " The aim of the development of ethanolamine aptamers is their use for the detection of this substance in clinical and environmental analysis. Ethanolamine is associated with several diseases. Moreover, ethanolamine and its derivatives di- and tri-ethanolamine are used in chemical and cosmetic industries. The use of biosensors with ethanolamine aptamer as new molecular recognition element could be an innovative method for an easy and fast detection of ethanolamine"
Reference (2005, Biochem Biophys Res Commun ): Mann, D., Reinemann, C., Stoltenburg, R., & Strehlitz, B. (2005). In vitro selection of DNA aptamers binding ethanolamine. Biochemical and biophysical research communications, 338(4), 1928–1934. https://doi.org/10.1016/j.bbrc.2005.10.172