RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: 2
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUAGACGAUGUUAUCAGGGAGUUGGGAUCAUAUAGUCUUACAUAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.4366197183098591
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 4
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUACGCAAGAGCCGCCCUAAUGGUUCAAUGGUAACUGUAUAUGAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.4788732394366197
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 6
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUAGACUUCUUGUGCCAUUAUGAAUUAUUGCUAAUCCUCUUGAAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.4084507042253521
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 8.6
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUAAGGACGUACUUGGAAAAGAGGCGCGAAGAACCUGGUAUGUAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.4929577464788732
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 9
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUAUAGGACGUACUUGGAAAAGAGGCGCGAAGAACCUGGUAUGAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.4929577464788732
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 10
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUAUGGCCACCUUGCCACUCUUCCUUGCAUAUUUUACUCCCGCAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.5070422535211268
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 11.7
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGGACGAUGUUACAAGCCGAGGGGGAGUAUCUGAUGACAAUUCGGAGCUCCAAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.5211267605633803
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 13.2
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUAUCAUGGUGUGUGAGUUAGCUCACGUGCCGUUUCGAAGGCGAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.5211267605633803
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 17.9
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUACAUGGGCUAGACCGGCAUAAAACUGCUGUAGUUGCACGCCAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.5211267605633803
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 20.4
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUACGUUGUAGUAGUGGCUUGGGCAUAACUCAGUUAAACACUAAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.4507042253521127
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 15
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUAGACCGCGGAAAAGGAAGGAAUUAGAUACAACGGAGAAGUGAAGAAGACUCGCAAGA3′
Sequence length: 71
GC content: 0.4788732394366197
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: T1.7.5
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUACGCAUCGUUUGCGUGGCGUGUCCGGGCGCAAGAAGACUCGCAAGA3′
Sequence length: 60
GC content: 0.6
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: 10 nucleotides of 3′-end truncated aptamer derived from oligonucleotide 7.5
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: T2.13.2
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: 2'-amino-RNA
Sequence: 5′GGGAGGACGAUGUUAUGGUGUGUGAGUUAGCUCACGUGCCGAAGAAGACUCGCAAGA3′
Sequence length: 57
GC content: 0.5263157894736842
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: 3 nucleotides from 5' end and 11 nucleotides from 3' end truncated aptamer derived from oligonucleotide 13.2; Up to 9 additional nucleotides could be removed from the 5′-end with little or no effect on binding affinity and modified with 2′-nh2-pyrimidine groups
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Additional information: Modfied pool of 2'-NH2-RNA --> .2'-NH2 ,-modified CTP and UTP
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: T3.11.7
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: 2'-amino-RNA
Sequence: 5′GGGAGGACGAUGUUAGGAGUAUCUGAUGACAAUUCGGAGCUCCAAGAAGACUCGCAAGA3′
Sequence length: 59
GC content: 0.4915254237288136
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: 11 nucleotides of 5′- and 1 nucleotide from 3′-end truncated aptamer, derived from clone 11.7 And modified with 2′-f-pyrimidine and 2′-nh2-pyrimidine groups
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Additional information: Modfied pool of 2'-NH2-RNA --> .2'-NH2 ,-modified CTP and UTP
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: T4.14.8
Target: Tumor necrosis factor alpha (hTNFα), Human
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGUUACGUAUAUACGGAUUAGGUUGUAGCUCAGACCAAGAAGACUCGCAAGA3′
Sequence length: 62
GC content: 0.4677419354838709
Affinity: Not reported
Pool type: 5′-GGGAGGACGATGTTA-N40-AAGAAGACTCGCAAGA-3′
Pool random region: 40
Binding buffer/conditions: PBS with 1 mM MgCl2 and 10 mM DTT
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: 9 nucelotides from 3' end truncated aptamer derived from oligonucleotide 14.8; Removal of an additional base pair from the base of the proposed stem (6 nucleotides of 5′-end and 5 of 3′-end derived from clone 14) did not affect the binding affinity.
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "Human tumor necrosis factor α (hTNFα), a pleiotropic cytokine with activities ranging from host defense mechanisms in infection and injury to severe toxicity in septic shock or other related diseases, is a promising target for drug screening. Oligonucleotide aptamers described here are potential therapeutics and diagnostics for hTNFα-related diseases."
Reference (2004, Genomics Proteomics Bioinformatics ): Yan, X., Gao, X., & Zhang, Z. (2004). Isolation and characterization of 2'-amino-modified RNA aptamers for human TNFalpha. Genomics, proteomics & bioinformatics, 2(1), 32–42. https://doi.org/10.1016/s1672-0229(04)02005-4
Aptamer: 4.4.2
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGCGGCGCGGGGTCTTTTACAAATAGTTGTCCTAGCCACGCCTATGCGTGCTACCGTGAA3′
Sequence length: 78
GC content: 0.5512820512820513
Affinity: Kd: 30 ± 22 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.3
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGACTATTGCACCTGTTCGACCTAACGTTGGTTTGGTAGGCCCTATGCGTGCTACCGTGAA3′
Sequence length: 79
GC content: 0.5189873417721519
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.4
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGACATTTCACAGCGTTCATAGTAACTGGCACTACAGTGCCGCCTGTGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.525
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.5
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGCGATAGTGGTCGATTCTCCAACTTTGTCTATGTGCACTTGCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.5
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v