RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: KHG11
Target: Acute myeloid leukemia cells (HL60)
Type of nucleic acid: ssDNA
Sequence: 5'ATCCAGAGTGACGCAGCATGCTCATCCACGATTCTGGCGAATTTAGTGCCTGTCTTTTTCTCTTGGACACGGTGGCTTAGT3'
Sequence length: 81
GC content: 0.4938271604938271
Affinity: Kd: 6.1 + 2.1 nM
Pool type: 5′-FITC- ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3′
Pool random region: 45
Binding buffer/conditions: 4.5 g/liter glucose, 5 mM MgCl2, 0.1 mg/ml tRNA and 1mg/ml BSA, all dissolved in Dulbecco's PBS with magnesium chloride and calcium chloride
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "Cell surface proteins can play important roles in cancer pathogenesis. Selected aptamers can be used as a molecular tool for further understanding surface protein expression patterns on tumor cells and thus providing a foundation for effective molecular analysis of leukemia and its subcategories; tumor diagnosis, classification and therapy"
Reference (2009, Leukemia ): Sefah, K., Tang, Z. W., Shangguan, D. H., Chen, H., Lopez-Colon, D., Li, Y., Parekh, P., Martin, J., Meng, L., Phillips, J. A., Kim, Y. M., & Tan, W. H. (2009). Molecular recognition of acute myeloid leukemia using aptamers. Leukemia, 23(2), 235–244. https://doi.org/10.1038/leu.2008.335
Aptamer: KH2B05
Target: Acute myeloid leukemia cells (HL60)
Type of nucleic acid: ssDNA
Sequence: 5'ATCCAGAGTGACGCAGCACACACAACCTGCTCATAAACTTTACTCTGCTCGAACCATCTCTGGACACGGTGGCTTAGT3'
Sequence length: 78
GC content: 0.5
Affinity: Kd: 184.5 ± 20.2 nM
Pool type: 5′-FITC- ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3′
Pool random region: 45
Binding buffer/conditions: 4.5 g/liter glucose, 5 mM MgCl2, 0.1 mg/ml tRNA and 1mg/ml BSA, all dissolved in Dulbecco's PBS with magnesium chloride and calcium chloride
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "Cell surface proteins can play important roles in cancer pathogenesis. Selected aptamers can be used as a molecular tool for further understanding surface protein expression patterns on tumor cells and thus providing a foundation for effective molecular analysis of leukemia and its subcategories; tumor diagnosis, classification and therapy"
Reference (2009, Leukemia ): Sefah, K., Tang, Z. W., Shangguan, D. H., Chen, H., Lopez-Colon, D., Li, Y., Parekh, P., Martin, J., Meng, L., Phillips, J. A., Kim, Y. M., & Tan, W. H. (2009). Molecular recognition of acute myeloid leukemia using aptamers. Leukemia, 23(2), 235–244. https://doi.org/10.1038/leu.2008.335
Aptamer: KH1A02
Target: Acute myeloid leukemia cells (HL60)
Type of nucleic acid: ssDNA
Sequence: 5'ATCCAGAGTGACGCAGCAGGCATAGATGTGCAGCTCCAAGGAGAAGAAGGAGTTCTGTGTATTGGACACGGTGGCTTAGT3'
Sequence length: 80
GC content: 0.5125
Affinity: Kd: 106.1 ± 15.4 nM
Pool type: 5′-FITC- ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3′
Pool random region: 45
Binding buffer/conditions: 4.5 g/liter glucose, 5 mM MgCl2, 0.1 mg/ml tRNA and 1mg/ml BSA, all dissolved in Dulbecco's PBS with magnesium chloride and calcium chloride
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "Cell surface proteins can play important roles in cancer pathogenesis. Selected aptamers can be used as a molecular tool for further understanding surface protein expression patterns on tumor cells and thus providing a foundation for effective molecular analysis of leukemia and its subcategories; tumor diagnosis, classification and therapy"
Reference (2009, Leukemia ): Sefah, K., Tang, Z. W., Shangguan, D. H., Chen, H., Lopez-Colon, D., Li, Y., Parekh, P., Martin, J., Meng, L., Phillips, J. A., Kim, Y. M., & Tan, W. H. (2009). Molecular recognition of acute myeloid leukemia using aptamers. Leukemia, 23(2), 235–244. https://doi.org/10.1038/leu.2008.335
Aptamer: KK1B10
Target: Acute myeloid leukemia cells (HL60)
Type of nucleic acid: ssDNA
Sequence: 5'ATCCAGAGTGACGCAGCAGATCAGTCTATCTTCTCCTGATGGGTTCCTATTTATAGGTGAAGCTGGACACGGTGGCTTAGT3'
Sequence length: 81
GC content: 0.4814814814814814
Affinity: Kd: 256.0 ± 72.0 nM
Pool type: 5′-FITC- ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3′
Pool random region: 45
Binding buffer/conditions: 4.5 g/liter glucose, 5 mM MgCl2, 0.1 mg/ml tRNA and 1mg/ml BSA, all dissolved in Dulbecco's PBS with magnesium chloride and calcium chloride
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "Cell surface proteins can play important roles in cancer pathogenesis. Selected aptamers can be used as a molecular tool for further understanding surface protein expression patterns on tumor cells and thus providing a foundation for effective molecular analysis of leukemia and its subcategories; tumor diagnosis, classification and therapy"
Reference (2009, Leukemia ): Sefah, K., Tang, Z. W., Shangguan, D. H., Chen, H., Lopez-Colon, D., Li, Y., Parekh, P., Martin, J., Meng, L., Phillips, J. A., Kim, Y. M., & Tan, W. H. (2009). Molecular recognition of acute myeloid leukemia using aptamers. Leukemia, 23(2), 235–244. https://doi.org/10.1038/leu.2008.335
Aptamer: KK1D04
Target: Acute myeloid leukemia cells (HL60)
Type of nucleic acid: ssDNA
Sequence: 5'ATCCAGAGTGACGCAGCACAAAGTCTCTTCGGCGCGAATCAGTTCATCTTTCCCTGATGGGGGTGGACACGGTGGCTTAGT3'
Sequence length: 81
GC content: 0.5432098765432098
Affinity: Kd: 204.6 ± 48.4 nM
Pool type: 5′-FITC- ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3′
Pool random region: 45
Binding buffer/conditions: 4.5 g/liter glucose, 5 mM MgCl2, 0.1 mg/ml tRNA and 1mg/ml BSA, all dissolved in Dulbecco's PBS with magnesium chloride and calcium chloride
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Therapeutic: "Cell surface proteins can play important roles in cancer pathogenesis. Selected aptamers can be used as a molecular tool for further understanding surface protein expression patterns on tumor cells and thus providing a foundation for effective molecular analysis of leukemia and its subcategories; tumor diagnosis, classification and therapy"
Reference (2009, Leukemia ): Sefah, K., Tang, Z. W., Shangguan, D. H., Chen, H., Lopez-Colon, D., Li, Y., Parekh, P., Martin, J., Meng, L., Phillips, J. A., Kim, Y. M., & Tan, W. H. (2009). Molecular recognition of acute myeloid leukemia using aptamers. Leukemia, 23(2), 235–244. https://doi.org/10.1038/leu.2008.335
Aptamer: SM-20
Target: Serine protease inhibitor plasminogen activator inhibitor-1 (PAI-1)
Type of nucleic acid: ssRNA
Sequence: 5′GGGAGGACGAUGCGGAGCGACUGACGAUCUUGAGUAAACCGCUCAUCCACGUAGUCAGACGACUCGCUGAGGAUCC3′
Sequence length: 76
GC content: 0.5789473684210527
Affinity: Kd: 920 pM
Pool type: 5′-GGGAGGACGATGCGG-N40-CAGACGACTCGCTGAGGATCC-3′
Pool random region: 40
Binding buffer/conditions: BSA binding buffer E (20 mM Hepes, pH 7.4, 50 mM NaCl, 2 mM CaCl2, and 0.1% bovine serum albumin [BSA]); CHAPS binding buffer E (20 mM Hepes, pH 7.4, 50 mM NaCl, 2 mM CaCl2, and 0.05% CHAPS); CHAPS binding buffer F (20 mM Hepes, pH 7.4, 150 mM NaCl, 2 mM CaCl2, and 0.05% CHAPS)
Divalent salt: CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " The serine protease inhibitor plasminogen activator inhibitor-1 (PAI-1) is increased in several cancers, including breast, where it is associated with a poor outcome. Therefore, the PAI-1 aptamer SM-20 demonstrates therapeutic potential as an antimetastatic agent and could possibly be used as an adjuvant to traditional chemotherapy for breast cancer."
Additional information: Different buffers were used during different rounds of selection
Reference (2009, Oligonucleotides ): Blake, C. M., Sullenger, B. A., Lawrence, D. A., & Fortenberry, Y. M. (2009). Antimetastatic potential of PAI-1-specific RNA aptamers. Oligonucleotides, 19(2), 117–128. https://doi.org/10.1089/oli.2008.0177
Aptamer: BC15
Target: Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1)
Type of nucleic acid: ssDNA
Sequence: 5'GCAATGGTACGGTACTTCCTGTGGCGAGGTAGGTGGGGTGTGTGTGTATCCAAAAGTGCACGCTACTTTGCTAA3'
Sequence length: 74
GC content: 0.5135135135135135
Affinity: Kd: 111.0 ± 36.9 nM
Pool type: 5'-GCAATGGTACGGTACTTCC-N30-CAAAAGTGCACGCTACTTTGCTAA-3'
Pool random region: 30
Binding buffer/conditions: PBS binding buffer
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " We report a new in situ tissue slide-based SELEX strategy targeting neoplastic tissues from breast cancer patients. The methodology, using the molecular differences between clinical specimens, can evolve aptamers to all fractions of tissue. The aptamers may be used as new molecular probes for pathological diagnosis and tumour imaging, and also to reveal the molecular differences that are responsible for the diseases."
Reference (2009, J Pathol ): Li, S., Xu, H., Ding, H., Huang, Y., Cao, X., Yang, G., Li, J., Xie, Z., Meng, Y., Li, X., Zhao, Q., Shen, B., & Shao, N. (2009). Identification of an aptamer targeting hnRNP A1 by tissue slide-based SELEX. The Journal of pathology, 218(3), 327–336. https://doi.org/10.1002/path.2543
Aptamer: BC-15
Target: Circulating tumor cells (CTCs)
Type of nucleic acid: ssDNA
Sequence: 5'GCAATGGTACGGTACTTCCTGTGGCGAGGTAGGTGGGGTGTGTGTGTATCCAAAAGTGCACGCTACTTTGCTAA3'
Sequence length: 74
GC content: 0.5135135135135135
Affinity: Kd: 111.0 ± 36.9 nM
Pool type: 5′‐GCAATGGTACGG‐TACTTCC‐N30‐CAAAAGTGCACGCTACTTTGC‐TAA‐3′
Pool random region: 30
Binding buffer/conditions: PBS buffer
Buffer type: Not Reported
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " Many studies have shown that the quantity and dynamics of circulating tumor cells (CTCs) in peripheral blood of patients afflicted with solid tumours have great relevance in therapeutic efficacy and prognosis. The aptamer method was compared with the well-established anti-cytokeratin method using 15 pancreatic cancer patient blood samples, and enumeration indicated no difference between these two methods. Our study establishes a novel way to identify CTCs by using a synthetic aptamer probe. This new approach is comparable with the anti-cytokeratin-based CTC identification method."
Additional information: affinity and pool type found here: https://pubmed-ncbi-nlm-nih-gov.ezproxy.lib.utexas.edu/19291713/
Reference (2015, PLoS One ): Zhang, J., Li, S., Liu, F., Zhou, L., Shao, N., & Zhao, X. (2015). SELEX aptamer used as a probe to detect circulating tumor cells in peripheral blood of pancreatic cancer patients. PloS one, 10(3), e0121920. https://doi.org/10.1371/journal.pone.0121920
Aptamer: RNA11F7t
Target: Factor Xa
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5′GAGAG(2′FC)(2′FC)(2′FC)(2′FC)AG(2′FC)GAGA(2′FU)AA(2′FU)A(2′FC)(2′FU)(2′FU)GG(2′FC)(2′FC)(2′FC)(2′FC)G(2′FC)(2′FU)(2′FC)(2′FU)(2′FU)U3'
Sequence length: 37
GC content: 0.5675675675675675
Affinity: Kd: 1.1 ± 0.2 nM
Pool type: 5′-GGGAGAGAGGAAGAGGGATGGG-N40-CATAACCCAGAGGTCGATAGTACTGGATCCCCCC-3′
Pool random region: 40
Binding buffer/conditions: 20 mm Hepes, 0.05 m NaCl, 2 mm CaCl2, 0.01% (w/v) bovine serum albumin, pH 7.4
Divalent salt: CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: The lead molecule was systematically shortened to obtain a truncated version (RNA11F7t) that retained binding activity. In parallel studies point mutations were introduced to yield RNAMUT with a greatly reduced ability to bind Xa. Ribooligonucleotides were chemically synthesized by Dharmacon Research and supplied desalted after deprotection form 2′-hydroxylated purines. 
Application as quoted in the referenced paper: Inhibition: "The interaction of factor Xa with factor Va on membranes to form prothrombinase profoundly increases the rate of the proteolytic conversion of prothrombin to thrombin. Thus, despite the complexity of the macromolecular interactions that underlie the assembly of prothrombinase, efficient inhibition of enzyme complex assembly and thrombin formation can be achieved by tight binding ligands that target factor Xa in a discrete manner"
Additional information: 2′FC is 2′-flurocytosine, 2′FU is 2′flurouracil, and idT denotes inverted deoxythymidine. The 2′-fluoro derivatives of cytidine triphosphate and uridine triphosphate (TriLink Biotechnologies) were incorporated into RNA libraries by in vitro transcription as described
Reference (2009, J Biol Chem ): Buddai, S. K., Layzer, J. M., Lu, G., Rusconi, C. P., Sullenger, B. A., Monroe, D. M., & Krishnaswamy, S. (2010). An anticoagulant RNA aptamer that inhibits proteinase-cofactor interactions within prothrombinase. The Journal of biological chemistry, 285(8), 5212–5223. https://doi.org/10.1074/jbc.M109.049833
Aptamer: 13.56
Target: Immunoglobulin E (IgE)-binding epitope of Asp f 1 allergen
Type of nucleic acid: ssDNA
Sequence: 5'TACTAACGGTACAAGCTACACACCCGCCCCGCTCCATCCGCCCATCGTCACCTACCCACCTCGTGATCCCCTTCCCCCAACGTTGACCTAGAAGC3'
Sequence length: 95
GC content: 0.6
Affinity: Kd 93.62 ± 21.84 nM
Pool type: 5′-TACTAACGGTACAAGCTA-N60-AACGTTGACCTAGAAGC-3'
Pool random region: 60
Binding buffer/conditions: Not reported
Buffer type: Not Reported
Molecular weight of target: 16.8 Kda
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " The objective of the research is to develop a DNA aptamer for the Asp f 1 allergen of the pathogenic fungus Aspergillus fumigatus, using an IgE-binding epitope of the allergen as the target for aptamer selection. Production of this aptamer provides proof-of-principle that allergen measurement methods can be developed to indicate the potent fraction, or allergenicity, of allergens."
Reference (2009, Biochem Biophys Res Commun ): Low, S. Y., Hill, J. E., & Peccia, J. (2009). A DNA aptamer recognizes the Asp f 1 allergen of Aspergillus fumigatus. Biochemical and biophysical research communications, 386(3), 544–548. https://doi.org/10.1016/j.bbrc.2009.06.089
Aptamer: No. 8
Target: 8-hydroxy-2′-deoxyguanosine (8-OHdG)
Type of nucleic acid: ssDNA
Sequence: 5′TAGGGAATTCGTCGACGGATCCCCCCTATAGTGAGTCGTATTAAATCCATATGACTAGTAGATCCTCTAGAGTCGACCTCTGCAGGTCGACGCATGCGCCG3′
Sequence length: 101
GC content: 0.5148514851485149
Affinity: Not reported
Pool type: 5′-TAGGGAATTCGTCGACGGATCC-N60-CTGCAGGTCGACGCATGCGCCG-3′
Pool random region: 60
Binding buffer/conditions: 50 mM Tris–HCl (pH 7.6), 300 mM NaCl, 30 mM KCl, and 5 mM MgCl2, 3.5 mM 8-OHdG
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " DNA aptamers, which bind specific molecule, such as 8-OHdG, with high affinity were investigated using an in vitro selection strategy called systematic evolution of ligands by exponential enrichment (SELEX). This result suggests that using an analogue will be a useful new SELEX method for obtaining various aptamers that are difficult to immobilize on a matrix."
Reference (2009, Bioorg Med Chem Lett ): Miyachi, Y., Shimizu, N., Ogino, C., Fukuda, H., & Kondo, A. (2009). Selection of a DNA aptamer that binds 8-OHdG using GMP-agarose. Bioorganic & medicinal chemistry letters, 19(13), 3619–3622. https://doi.org/10.1016/j.bmcl.2009.04.130
Aptamer: No. 11
Target: 8-hydroxy-2′-deoxyguanosine (8-OHdG)
Type of nucleic acid: ssDNA
Sequence: 5′TAGGGAATTCGTCGACGGATCCGCGTCGACCTGCAGACCCCCATATCATCAGTCCCACCCCCAGGCACAGAGCGCCGTACCCCCCTGCAGGTCGACGCATGCGCCG3′
Sequence length: 106
GC content: 0.660377358490566
Affinity: Not reported
Pool type: 5′-TAGGGAATTCGTCGACGGATCC-N60-CTGCAGGTCGACGCATGCGCCG-3′
Pool random region: 60
Binding buffer/conditions: 50 mM Tris–HCl (pH 7.6), 300 mM NaCl, 30 mM KCl, and 5 mM MgCl2, 3.5 mM 8-OHdG
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " DNA aptamers, which bind specific molecule, such as 8-OHdG, with high affinity were investigated using an in vitro selection strategy called systematic evolution of ligands by exponential enrichment (SELEX). This result suggests that using an analogue will be a useful new SELEX method for obtaining various aptamers that are difficult to immobilize on a matrix."
Reference (2009, Bioorg Med Chem Lett ): Miyachi, Y., Shimizu, N., Ogino, C., Fukuda, H., & Kondo, A. (2009). Selection of a DNA aptamer that binds 8-OHdG using GMP-agarose. Bioorganic & medicinal chemistry letters, 19(13), 3619–3622. https://doi.org/10.1016/j.bmcl.2009.04.130
Aptamer: No. 22
Target: 8-hydroxy-2′-deoxyguanosine (8-OHdG)
Type of nucleic acid: ssDNA
Sequence: 5′TAGGGAATTCGTCGACGGATCCGCGGGCGATCGGCGGGGGGTGCGTGCGCTCTGTGCCAGGGGGTGGGACAGATCATATGGGGGTGCTCTGCAGGTCGACGCATGCGCCG3′
Sequence length: 110
GC content: 0.6818181818181818
Affinity: Kd: 0.1 μmol/L
Pool type: 5′-TAGGGAATTCGTCGACGGATCC-N60-CTGCAGGTCGACGCATGCGCCG-3′
Pool random region: 60
Binding buffer/conditions: 50 mM Tris–HCl (pH 7.6), 300 mM NaCl, 30 mM KCl, and 5 mM MgCl2, 3.5 mM 8-OHdG
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " DNA aptamers, which bind specific molecule, such as 8-OHdG, with high affinity were investigated using an in vitro selection strategy called systematic evolution of ligands by exponential enrichment (SELEX). This result suggests that using an analogue will be a useful new SELEX method for obtaining various aptamers that are difficult to immobilize on a matrix."
Reference (2009, Bioorg Med Chem Lett ): Miyachi, Y., Shimizu, N., Ogino, C., Fukuda, H., & Kondo, A. (2009). Selection of a DNA aptamer that binds 8-OHdG using GMP-agarose. Bioorganic & medicinal chemistry letters, 19(13), 3619–3622. https://doi.org/10.1016/j.bmcl.2009.04.130
Aptamer: Ars-3
Target: Arsenate [(As(V)]
Type of nucleic acid: ssDNA
Sequence: 5′GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATTTTACAGAACAACCAACGTCGTCTCCGGGTACTTCTTCATCGAGATAGTAAGTGCAATCT3′
Sequence length: 101
GC content: 0.4158415841584158
Affinity: Kd: 4.95± 0.31 nM
Pool type: 5′-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3′
Pool random region: 40
Binding buffer/conditions: 50 mM HEPES, 150 mM NaCl, 0.5 mM MgCl2, 0.5 mM CaCl2
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Single-stranded DNA aptamers were generated from a random library to remove arsenic from Vietnamese groundwater. The specific affinity interactions oftheArs-3 aptamer to arsenic were verified against other heavy metals. After obtaining successful removal results with a laboratory-prepared aqueous arsenic solution, Ars-3 was applied for removal of any arsenic present in the groundwater samples collected from the studied areas in Vietnam. Field results were also successful: various arsenic concentrations ranging from 28.1 to 739.2 µg/L were completely removed after 5 min of incubation with the arsenic-binding aptamer Ars-3."
Reference (2009, Environ Sci Technol ): Kim, M., Um, H. J., Bang, S., Lee, S. H., Oh, S. J., Han, J. H., Kim, K. W., Min, J., & Kim, Y. H. (2009). Arsenic removal from Vietnamese groundwater using the arsenic-binding DNA aptamer. Environmental science & technology, 43(24), 9335–9340. https://doi.org/10.1021/es902407g
Aptamer: Ars-3
Target: Arsenite [As(III)]
Type of nucleic acid: ssDNA
Sequence: 5′GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATTTTACAGAACAACCAACGTCGTCTCCGGGTACTTCTTCATCGAGATAGTAAGTGCAATCT3′
Sequence length: 101
GC content: 0.4158415841584158
Affinity: Kd: 7.05 ± 0.91 nM 
Pool type: 5′-GGTAATACGACTCACTATAGGGAGATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3′
Pool random region: 40
Binding buffer/conditions: 50 mM HEPES, 150 mM NaCl, 0.5 mM MgCl2, 0.5 mM CaCl2
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Single-stranded DNA aptamers were generated from a random library to remove arsenic from Vietnamese groundwater. The specific affinity interactions oftheArs-3 aptamer to arsenic were verified against other heavy metals. After obtaining successful removal results with a laboratory-prepared aqueous arsenic solution, Ars-3 was applied for removal of any arsenic present in the groundwater samples collected from the studied areas in Vietnam. Field results were also successful: various arsenic concentrations ranging from 28.1 to 739.2 µg/L were completely removed after 5 min of incubation with the arsenic-binding aptamer Ars-3."
Reference (2009, Environ Sci Technol ): Kim, M., Um, H. J., Bang, S., Lee, S. H., Oh, S. J., Han, J. H., Kim, K. W., Min, J., & Kim, Y. H. (2009). Arsenic removal from Vietnamese groundwater using the arsenic-binding DNA aptamer. Environmental science & technology, 43(24), 9335–9340. https://doi.org/10.1021/es902407g
Aptamer: b02
Target: Plasmodium falciparum (P. falciparum) erythrocyte membrane protein 1 (PfEMP1)
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5′CGACUGCAGAGCUUGCUACGUAGCUCACCACACCAGCAAUCAACGCGUUUUUUCAAACACUGGCACAUGAGGUACCGAGCUCGAAUUCCC3′
Sequence length: 90
GC content: 0.5222222222222223
Affinity: Not reported
Pool type: 5′-CGACTGCAGAGCTTGCTACG-N50-GGTACCGAGCTCGAATTCCC-3′
Pool random region: 50
Binding buffer/conditions: PBSM (PBS, 1 mM MgCl2)
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " The var-gene encoding Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is known to play a major role in the pathogenicity of the P. falciparum parasite. The protein enables the parasite to adhere to the endothelial linings of small blood vessels (cytoadherence) as well as to non-infected erythrocytes (rosetting), thus preventing clearance from the bloodstream. The described results show that RNA aptamers are promising candidates for adjunct therapy in severe malaria."
Additional information: DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
The DNA library was generated using oligo B (5′-CGACTGCAGAGCTTGCTACG (N)50 GGTACCGAGCTCGAATTCCC-3′) and oligo A (5′-GCGTAATACGACTCACTATAGGGAATTCGAGCTCGGTACC-3′), sequence for T7 promoter underlined.
2′F-modified library (ATP, GTP, 2′F-dCTP, 2′F-dUTP)
Reference (2009, Parasitol Res ): Barfod, A., Persson, T., & Lindh, J. (2009). In vitro selection of RNA aptamers against a conserved region of the Plasmodium falciparum erythrocyte membrane protein 1. Parasitology research, 105(6), 1557–1566. https://doi.org/10.1007/s00436-009-1583-x