RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: 9
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'GCUCUUGGGCGCAGCCUCAAUGAGGCUGGUGGUGCAAG3'
Sequence length: 38
GC content: 0.631578947368421
Affinity: Kd: 1.9 ± 0.21*
Pool type: 5'-GCUCUUG-N4-CAGCCUCAAUGAGGCUG-N6-CAAG-3'
Pool random region: 10
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units. 

Sequences at the defined 5' and 3' ends of the 76.6 DNA pool
are, respectively, 5'-GGTAATACGATCACTATAGGG4ACTC_x0002_GATGAAGCGAGCT-3' and 5'-TACTGACT7CGGCATCCCTGC-
-(3')
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 18
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'GCUCUUGGGCGCAGCCUCAAUGAGGCUGGAGGUACAAG3'
Sequence length: 38
GC content: 0.6052631578947368
Affinity: Kd: 2.0*
Pool type: 5'-GCUCUUG-N4-CAGCCUCAAUGAGGCUG-N6-CAAG-3'
Pool random region: 10
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

Sequences at the defined 5' and 3' ends of the 76.6 DNA pool
are, respectively, 5'-GGTAATACGATCACTATAGGG4ACTC_x0002_GATGAAGCGAGCT-3' and 5'-TACTGACT7CGGCATCCCTGC-
-(3')
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 19
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'GCUCUUGGGCACAGCCUCAAUGAGGCUGGUGGUACAAG3'
Sequence length: 38
GC content: 0.5789473684210527
Affinity: Kd: 1.2 ± 0.08*
Pool type: 5'-GCUCUUG-N4-CAGCCUCAAUGAGGCUG-N6-CAAG-3'
Pool random region: 10
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

Sequences at the defined 5' and 3' ends of the 76.6 DNA pool
are, respectively, 5'-GGTAATACGATCACTATAGGG4ACTC_x0002_GATGAAGCGAGCT-3' and 5'-TACTGACT7CGGCATCCCTGC-
-(3')
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 6
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'GCUCUUGGACACAGCCUCAAUGAGGCUGCAGAUACAAG3'
Sequence length: 38
GC content: 0.5263157894736842
Affinity: Kd: 3.1 ± 0.26*
Pool type: 5'-GCUCUUG-N4-CAGCCUCAAUGAGGCUG-N6-CAAG-3'
Pool random region: 10
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

Sequences at the defined 5' and 3' ends of the 76.6 DNA pool
are, respectively, 5'-GGTAATACGATCACTATAGGG4ACTC_x0002_GATGAAGCGAGCT-3' and 5'-TACTGACT7CGGCATCCCTGC-
-(3')
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 116
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'GCUCUUGGACACAGCUGCUGCAGAUACAAG3'
Sequence length: 30
GC content: 0.5333333333333333
Affinity: Kd: 2.1*
Pool type: 5'-GCUCUUG-N4-CAGCCUCAAUGAGGCUG-N6-CAAG-3'
Pool random region: 10
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

Sequences at the defined 5' and 3' ends of the 76.6 DNA pool
are, respectively, 5'-GGTAATACGATCACTATAGGG4ACTC_x0002_GATGAAGCGAGCT-3' and 5'-TACTGACT7CGGCATCCCTGC-
-(3')
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 126
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'GCUCUUGGACACAGCCUCAAUGAGGCUGCAGAAACAAG3'
Sequence length: 38
GC content: 0.5263157894736842
Affinity: Kd: 2.7 ± 0.29*
Pool type: 5'-GCUCUUG-N4-CAGCCUCAAUGAGGCUG-N6-CAAG-3'
Pool random region: 10
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

Sequences at the defined 5' and 3' ends of the 76.6 DNA pool
are, respectively, 5'-GGTAATACGATCACTATAGGG4ACTC_x0002_GATGAAGCGAGCT-3' and 5'-TACTGACT7CGGCATCCCTGC-
-(3')
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 15
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'GCUCUUGGCCGCAGCCUCAAUGAGGCUGAUGAUACAAG3'
Sequence length: 38
GC content: 0.5526315789473685
Affinity: Kd: 1.7*
Pool type: 5'-GCUCUUG-N4-CAGCCUCAAUGAGGCUG-N6-CAAG-3'
Pool random region: 10
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

Sequences at the defined 5' and 3' ends of the 76.6 DNA pool
are, respectively, 5'-GGTAATACGATCACTATAGGG4ACTC_x0002_GATGAAGCGAGCT-3' and 5'-TACTGACT7CGGCATCCCTGC-
-(3')
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 1
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'AUUCUGUACUCCGUACGCAAGUACGGUCGAGAAACAG3'
Sequence length: 37
GC content: 0.4864864864864865
Affinity: Kd: 4.3*
Pool type: 5'-AUUCUGU-N6.9-GUACGCAAGUAC-N6.9-ACAG-3'
Pool random region: 15
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

6.9 means Both random sequence tracts were systematically varied from 6 to 9 nts in length.

Sequences at the defined 5' and 3' ends of the 79.9
pool are, respectively, 5'-GGTAATACGACTCACTATAGGG_x0002_AACTCG4TG4AGCGAATT-3' and 5'-GCCTATCTATCGGAT_x0002_CCACG-3'.
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 2
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'AUUCUGUUUAGGACUCGUACGCAAGUACUGAGAUACUACAG3'
Sequence length: 41
GC content: 0.4146341463414634
Affinity: Kd: 2.4*
Pool type: 5'-AUUCUGU-N6.9-GUACGCAAGUAC-N6.9-ACAG-3'
Pool random region: 15
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

6.9 means Both random sequence tracts were systematically varied from 6 to 9 nts in length.

Sequences at the defined 5' and 3' ends of the 79.9
pool are, respectively, 5'-GGTAATACGACTCACTATAGGG_x0002_AACTCG4TG4AGCGAATT-3' and 5'-GCCTATCTATCGGAT_x0002_CCACG-3'.
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 14
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'AUUCUGGACUCGUACGCAAGUACUGGAGAAACAG3'
Sequence length: 34
GC content: 0.4705882352941176
Affinity: Kd: 5.4 ± 0.05*
Pool type: 5'-AUUCUGU-N6.9-GUACGCAAGUAC-N6.9-ACAG-3'
Pool random region: 15
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

6.9 means Both random sequence tracts were systematically varied from 6 to 9 nts in length.

Sequences at the defined 5' and 3' ends of the 79.9
pool are, respectively, 5'-GGTAATACGACTCACTATAGGG_x0002_AACTCG4TG4AGCGAATT-3' and 5'-GCCTATCTATCGGAT_x0002_CCACG-3'.
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 23
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'AUUCUGUGGACUCGUACGCAAGUACUUGAGAUACACG3'
Sequence length: 37
GC content: 0.4594594594594595
Affinity: Kd: 3.1*
Pool type: 5'-AUUCUGU-N6.9-GUACGCAAGUAC-N6.9-ACAG-3'
Pool random region: 15
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

6.9 means Both random sequence tracts were systematically varied from 6 to 9 nts in length.

Sequences at the defined 5' and 3' ends of the 79.9
pool are, respectively, 5'-GGTAATACGACTCACTATAGGG_x0002_AACTCG4TG4AGCGAATT-3' and 5'-GCCTATCTATCGGAT_x0002_CCACG-3'.
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 50
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'AUUCUGGUCUCGUACGCAAGUACUGAGAAACGACAG3'
Sequence length: 36
GC content: 0.4722222222222222
Affinity: Kd: 0.4*
Pool type: 5'-AUUCUGU-N6.9-GUACGCAAGUAC-N6.9-ACAG-3'
Pool random region: 15
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

6.9 means Both random sequence tracts were systematically varied from 6 to 9 nts in length.

Sequences at the defined 5' and 3' ends of the 79.9
pool are, respectively, 5'-GGTAATACGACTCACTATAGGG_x0002_AACTCG4TG4AGCGAATT-3' and 5'-GCCTATCTATCGGAT_x0002_CCACG-3'.
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 63
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'AUUCUGGACUCCGUAUGCAAGUACGUUGAGCAACAG3'
Sequence length: 36
GC content: 0.4722222222222222
Affinity: Kd: 7.7, 9.5, 9.6*
Pool type: 5'-AUUCUGU-N6.9-GUACGCAAGUAC-N6.9-ACAG-3'
Pool random region: 15
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

6.9 means Both random sequence tracts were systematically varied from 6 to 9 nts in length.

Sequences at the defined 5' and 3' ends of the 79.9
pool are, respectively, 5'-GGTAATACGACTCACTATAGGG_x0002_AACTCG4TG4AGCGAATT-3' and 5'-GCCTATCTATCGGAT_x0002_CCACG-3'.
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 74
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'AUUCUGUAGACUCGUACGCAAGUACUCGAGAUAUACAG3'
Sequence length: 38
GC content: 0.4210526315789473
Affinity: Kd: 4.2*
Pool type: 5'-AUUCUGU-N6.9-GUACGCAAGUAC-N6.9-ACAG-3'
Pool random region: 15
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

6.9 means Both random sequence tracts were systematically varied from 6 to 9 nts in length.

Sequences at the defined 5' and 3' ends of the 79.9
pool are, respectively, 5'-GGTAATACGACTCACTATAGGG_x0002_AACTCG4TG4AGCGAATT-3' and 5'-GCCTATCTATCGGAT_x0002_CCACG-3'.
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 83
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'AUUCUGUGGACUCGUACGCAAGUACUGAGAAACACCG3'
Sequence length: 37
GC content: 0.4864864864864865
Affinity: Kd: 3.6*
Pool type: 5'-AUUCUGU-N6.9-GUACGCAAGUAC-N6.9-ACAG-3'
Pool random region: 15
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

6.9 means Both random sequence tracts were systematically varied from 6 to 9 nts in length.

Sequences at the defined 5' and 3' ends of the 79.9
pool are, respectively, 5'-GGTAATACGACTCACTATAGGG_x0002_AACTCG4TG4AGCGAATT-3' and 5'-GCCTATCTATCGGAT_x0002_CCACG-3'.
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509
Aptamer: 88
Target: Rev protein of HIV-1 (minimal Rev-binding element (RBE) found within the Rev Responsive Element (RRE))
Type of nucleic acid: ssRNA
Sequence: 5'AUUCUGUGACUCUUUGUACGCAAGUACAGAGUGAUACAG3'
Sequence length: 39
GC content: 0.4102564102564102
Affinity: Kd: 1.4*
Pool type: 5'-AUUCUGU-N6.9-GUACGCAAGUAC-N6.9-ACAG-3'
Pool random region: 15
Binding buffer/conditions: 50 mM KCl, 50 mM Tris-Cl, pH 8.0
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " A population of improved Rev-binding aptamers can be used to inhibit viral
replication, as opposed to a single RNA species. In contrast to other pharmaceuticals, this approach will make it extremely difficult for HIV-1 to accumulate mutations that can simultaneously evade a multitude of different RRE decays."
Additional information: *Activity values are described as "representing relative Kd's" with no units

6.9 means Both random sequence tracts were systematically varied from 6 to 9 nts in length.

Sequences at the defined 5' and 3' ends of the 79.9
pool are, respectively, 5'-GGTAATACGACTCACTATAGGG_x0002_AACTCG4TG4AGCGAATT-3' and 5'-GCCTATCTATCGGAT_x0002_CCACG-3'.
Reference (1993, Nucleic Acids Res ): Giver, L., Bartel, D., Zapp, M., Pawul, A., Green, M., & Ellington, A. D. (1993). Selective optimization of the Rev-binding element of HIV-1. Nucleic acids research, 21(23), 5509–5516. https://doi.org/10.1093/nar/21.23.5509