RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: 56F
Target: Keratinocyte growth factor (hKGF), Human
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5'GGGAGGACGAUGCGGGGACAAWYAGCGGUGUCUUUUCAUUUNKAUCCUCCGACRUCCCAGACGACUCGCCCGA3'
Sequence length: 68
GC content: 0.5882352941176471
Affinity: Kd: biphasic 0.07 nM Ki: 0.3 nM
Pool type: 5'-GGGAGGACGATGCGG-N40-CAGACGACTCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: Ca++ and Mg++ containing Dulbeco's Phosphate Buffered Saline (DPBS, Life Technologies, Gaithersburg, MD) with 0.01% human serum albumin (Sigma, St. Louis, MO)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 26-28 kDa
Application as quoted in the referenced paper: Research: " RNA molecules with fluoro (2'F) or amino (2'NH2) substitution at the 2' position of pyrimidines are nuclease resistant' and can be synthesized by T7 RNA polymerase. Thus, SELEX experiments with libraries carrying such modifications can lead to nuclease-resistant ligands"
Additional information: all pyrimidines have 2' fluro substitutions; R=A or G, Y=C or U, K=G or U, M=A or C, V=G or C or A, W=A or U, S=G or C, D=G or T or A, and X=any residue, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1997, Nat Biotechnol ): Pagratis, N. C., Bell, C., Chang, Y. F., Jennings, S., Fitzwater, T., Jellinek, D., & Dang, C. (1997). Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nature biotechnology, 15(1), 68–73. https://doi.org/10.1038/nbt0197-68
Aptamer: 37F
Target: Keratinocyte growth factor (hKGF), Human
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5'GGGAGGACGAUGCGGGCCCGAUCUACUGCAUUACCGAAACGAUUUCCCCACUGUCAGACGACUCGCCCGA3'
Sequence length: 70
GC content: 0.6
Affinity: Kd: 0.46 nM Ki: 6.7 nM
Pool type: 5'-GGGAGGACGATGCGG-N40-CAGACGACTCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: Ca++ and Mg++ containing Dulbeco's Phosphate Buffered Saline (DPBS, Life Technologies, Gaithersburg, MD) with 0.01% human serum albumin (Sigma, St. Louis, MO)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 26-28 kDa
Application as quoted in the referenced paper: Research: " RNA molecules with fluoro (2'F) or amino (2'NH2) substitution at the 2' position of pyrimidines are nuclease resistant' and can be synthesized by T7 RNA polymerase. Thus, SELEX experiments with libraries carrying such modifications can lead to nuclease-resistant ligands"
Additional information: all pyrimidines have 2' fluro substitutions, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.

Reference (1997, Nat Biotechnol ): Pagratis, N. C., Bell, C., Chang, Y. F., Jennings, S., Fitzwater, T., Jellinek, D., & Dang, C. (1997). Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nature biotechnology, 15(1), 68–73. https://doi.org/10.1038/nbt0197-68
Aptamer: 13F
Target: Keratinocyte growth factor (hKGF), Human
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5'GGGAGGACGAUGCGGCACCUUAGACCUGUCCUCCAAGCGUGAGUUGCUGUGGCCCAGACGACUCGCCCGA3'
Sequence length: 70
GC content: 0.6428571428571429
Affinity: Kd: biphasic 0.03 nM Ki: 10.0 nM
Pool type: 5'-GGGAGGACGATGCGG-N40-CAGACGACTCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: Ca++ and Mg++ containing Dulbeco's Phosphate Buffered Saline (DPBS, Life Technologies, Gaithersburg, MD) with 0.01% human serum albumin (Sigma, St. Louis, MO)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 26-28 kDa
Application as quoted in the referenced paper: Research: " RNA molecules with fluoro (2'F) or amino (2'NH2) substitution at the 2' position of pyrimidines are nuclease resistant' and can be synthesized by T7 RNA polymerase. Thus, SELEX experiments with libraries carrying such modifications can lead to nuclease-resistant ligands"
Additional information: all pyrimidines have 2' fluro substitutions, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1997, Nat Biotechnol ): Pagratis, N. C., Bell, C., Chang, Y. F., Jennings, S., Fitzwater, T., Jellinek, D., & Dang, C. (1997). Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nature biotechnology, 15(1), 68–73. https://doi.org/10.1038/nbt0197-68
Aptamer: 50F
Target: Keratinocyte growth factor (hKGF), Human
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5'GGGAGGACGAUGCGGUGAUCAAUCGGCGCUUUACUCUUGCGCUCACCGUGCCCCAGACGACUCGCCCGA3'
Sequence length: 69
GC content: 0.6376811594202898
Affinity: Kd: biphasic 0.12 nM
Pool type: 5'-GGGAGGACGATGCGG-N40-CAGACGACTCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: Ca++ and Mg++ containing Dulbeco's Phosphate Buffered Saline (DPBS, Life Technologies, Gaithersburg, MD) with 0.01% human serum albumin (Sigma, St. Louis, MO)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 26-28 kDa
Application as quoted in the referenced paper: Research: " RNA molecules with fluoro (2'F) or amino (2'NH2) substitution at the 2' position of pyrimidines are nuclease resistant' and can be synthesized by T7 RNA polymerase. Thus, SELEX experiments with libraries carrying such modifications can lead to nuclease-resistant ligands"
Additional information: all pyrimidines have 2' fluro substitutions, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1997, Nat Biotechnol ): Pagratis, N. C., Bell, C., Chang, Y. F., Jennings, S., Fitzwater, T., Jellinek, D., & Dang, C. (1997). Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nature biotechnology, 15(1), 68–73. https://doi.org/10.1038/nbt0197-68
Aptamer: 38F
Target: Keratinocyte growth factor (hKGF), Human
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5'GGGAGGACGAUGCGGNGACUGAUUUUUCCUUGNCAGUGUAAUUUCCUGGCUGCCCCAGACGACUCGCCCGA3'
Sequence length: 69
GC content: 0.5797101449275363
Affinity: Kd: 0.33 nM Ki: 0.2 nM
Pool type: 5'-GGGAGGACGATGCGG-N40-CAGACGACTCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: Ca++ and Mg++ containing Dulbeco's Phosphate Buffered Saline (DPBS, Life Technologies, Gaithersburg, MD) with 0.01% human serum albumin (Sigma, St. Louis, MO)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 26-28 kDa
Application as quoted in the referenced paper: Research: " RNA molecules with fluoro (2'F) or amino (2'NH2) substitution at the 2' position of pyrimidines are nuclease resistant' and can be synthesized by T7 RNA polymerase. Thus, SELEX experiments with libraries carrying such modifications can lead to nuclease-resistant ligands"
Additional information: all pyrimidines have 2' fluro substitutions; R=A or G, Y=C or U, K=G or U, M=A or C, V=G or C or A, W=A or U, S=G or C, D=G or T or A, and X=any residue, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1997, Nat Biotechnol ): Pagratis, N. C., Bell, C., Chang, Y. F., Jennings, S., Fitzwater, T., Jellinek, D., & Dang, C. (1997). Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nature biotechnology, 15(1), 68–73. https://doi.org/10.1038/nbt0197-68
Aptamer: 10F
Target: Keratinocyte growth factor (hKGF), Human
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5'GGGAGGACGAUGCGGGACCAGAUGGCGGAUUUUUCAGCAAUCCUCCCCCGCUGCCCAGACGACUCGCCCGA3'
Sequence length: 71
GC content: 0.647887323943662
Affinity: Kd: 0.47 nM Ki: 10.0 nM
Pool type: 5'-GGGAGGACGATGCGG-N40-CAGACGACTCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: Ca++ and Mg++ containing Dulbeco's Phosphate Buffered Saline (DPBS, Life Technologies, Gaithersburg, MD) with 0.01% human serum albumin (Sigma, St. Louis, MO)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 26-28 kDa
Application as quoted in the referenced paper: Research: " RNA molecules with fluoro (2'F) or amino (2'NH2) substitution at the 2' position of pyrimidines are nuclease resistant' and can be synthesized by T7 RNA polymerase. Thus, SELEX experiments with libraries carrying such modifications can lead to nuclease-resistant ligands"
Additional information: all pyrimidines have 2' fluro substitutions, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1997, Nat Biotechnol ): Pagratis, N. C., Bell, C., Chang, Y. F., Jennings, S., Fitzwater, T., Jellinek, D., & Dang, C. (1997). Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nature biotechnology, 15(1), 68–73. https://doi.org/10.1038/nbt0197-68
Aptamer: 43F
Target: Keratinocyte growth factor (hKGF), Human
Type of nucleic acid: 2'-fluoro-RNA
Sequence: 5'GGGAGGACGAUGCGGUGUAGUUUCCCUGUAUGCCAGACGACUCGCCCGA3'
Sequence length: 49
GC content: 0.6122448979591837
Affinity: Kd: 1.13 nM Ki: 16.7 nM
Pool type: 5'-GGGAGGACGATGCGG-N40-CAGACGACTCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: Ca++ and Mg++ containing Dulbeco's Phosphate Buffered Saline (DPBS, Life Technologies, Gaithersburg, MD) with 0.01% human serum albumin (Sigma, St. Louis, MO)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 26-28 kDa
Application as quoted in the referenced paper: Research: " RNA molecules with fluoro (2'F) or amino (2'NH2) substitution at the 2' position of pyrimidines are nuclease resistant' and can be synthesized by T7 RNA polymerase. Thus, SELEX experiments with libraries carrying such modifications can lead to nuclease-resistant ligands"
Additional information: all pyrimidines have 2' fluro substitutions, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1997, Nat Biotechnol ): Pagratis, N. C., Bell, C., Chang, Y. F., Jennings, S., Fitzwater, T., Jellinek, D., & Dang, C. (1997). Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nature biotechnology, 15(1), 68–73. https://doi.org/10.1038/nbt0197-68
Aptamer: 14N
Target: Keratinocyte growth factor (hKGF), Human
Type of nucleic acid: 2'-amino-RNA
Sequence: 5'GGGAGGACGAUGCGGUCCAGGGAUUGAAGUGUCGGGGUAGGAACAUAAAGGCGGCCAGACGACUCGCCCGA3'
Sequence length: 71
GC content: 0.6197183098591549
Affinity: Kd: 0.4 nM Ki: 2.0 nM
Pool type: 5'-GGGAGGACGATGCGG-N40-CAGACGACTCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: Ca++ and Mg++ containing Dulbeco's Phosphate Buffered Saline (DPBS, Life Technologies, Gaithersburg, MD) with 0.01% human serum albumin (Sigma, St. Louis, MO)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 26-28 kDa
Application as quoted in the referenced paper: Research: " RNA molecules with fluoro (2'F) or amino (2'NH2) substitution at the 2' position of pyrimidines are nuclease resistant' and can be synthesized by T7 RNA polymerase. Thus, SELEX experiments with libraries carrying such modifications can lead to nuclease-resistant ligands"
Additional information: all pyrimidines have 2' amino substitutions, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1997, Nat Biotechnol ): Pagratis, N. C., Bell, C., Chang, Y. F., Jennings, S., Fitzwater, T., Jellinek, D., & Dang, C. (1997). Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nature biotechnology, 15(1), 68–73. https://doi.org/10.1038/nbt0197-68
Aptamer: 4N
Target: Keratinocyte growth factor (hKGF), Human
Type of nucleic acid: 2'-amino-RNA
Sequence: 5'GGGAGGACGAUGCGGGUGGUGAAGAGGUACCGGAAUUGCUAAAGAUACCACGGCCCAGACGACUCGCCCGA3'
Sequence length: 71
GC content: 0.6056338028169014
Affinity: Kd: 0.7 nM Ki: 23.3 nM
Pool type: 5'-GGGAGGACGATGCGG-N40-CAGACGACTCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: Ca++ and Mg++ containing Dulbeco's Phosphate Buffered Saline (DPBS, Life Technologies, Gaithersburg, MD) with 0.01% human serum albumin (Sigma, St. Louis, MO)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 26-28 kDa
Application as quoted in the referenced paper: Research: " RNA molecules with fluoro (2'F) or amino (2'NH2) substitution at the 2' position of pyrimidines are nuclease resistant' and can be synthesized by T7 RNA polymerase. Thus, SELEX experiments with libraries carrying such modifications can lead to nuclease-resistant ligands"
Additional information: all pyrimidines have 2' amino substitutions, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1997, Nat Biotechnol ): Pagratis, N. C., Bell, C., Chang, Y. F., Jennings, S., Fitzwater, T., Jellinek, D., & Dang, C. (1997). Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nature biotechnology, 15(1), 68–73. https://doi.org/10.1038/nbt0197-68
Aptamer: 1N
Target: Keratinocyte growth factor (hKGF), Human
Type of nucleic acid: 2'-amino-RNA
Sequence: 5'GGGAGGACGAUGCGGGAAGGGACGAUAAAGAGGAAUCGAACAACAAGUGGCUGGCCAGACGACUCGCCCGA3'
Sequence length: 71
GC content: 0.5915492957746479
Affinity: Kd: 0.5 nM Ki: 16.7 nM
Pool type: 5'-GGGAGGACGATGCGG-N40-CAGACGACTCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: Ca++ and Mg++ containing Dulbeco's Phosphate Buffered Saline (DPBS, Life Technologies, Gaithersburg, MD) with 0.01% human serum albumin (Sigma, St. Louis, MO)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 26-28 kDa
Application as quoted in the referenced paper: Research: " RNA molecules with fluoro (2'F) or amino (2'NH2) substitution at the 2' position of pyrimidines are nuclease resistant' and can be synthesized by T7 RNA polymerase. Thus, SELEX experiments with libraries carrying such modifications can lead to nuclease-resistant ligands"
Additional information: all pyrimidines have 2' amino substitutions, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1997, Nat Biotechnol ): Pagratis, N. C., Bell, C., Chang, Y. F., Jennings, S., Fitzwater, T., Jellinek, D., & Dang, C. (1997). Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nature biotechnology, 15(1), 68–73. https://doi.org/10.1038/nbt0197-68
Aptamer: 29N
Target: Keratinocyte growth factor (hKGF), Human
Type of nucleic acid: 2'-amino-RNA
Sequence: 5'GGGAGGACGAUGCGGAGAAGAAUGCAGGAAACAGCGAAAUGCGUGUGGCCAGACGACUCGCCCGA3'
Sequence length: 65
GC content: 0.6
Affinity: Kd: 0.43 nM Ki: 13.3 nM
Pool type: 5'-GGGAGGACGATGCGG-N40-CAGACGACTCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: Ca++ and Mg++ containing Dulbeco's Phosphate Buffered Saline (DPBS, Life Technologies, Gaithersburg, MD) with 0.01% human serum albumin (Sigma, St. Louis, MO)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 26-28 kDa
Application as quoted in the referenced paper: Research: " RNA molecules with fluoro (2'F) or amino (2'NH2) substitution at the 2' position of pyrimidines are nuclease resistant' and can be synthesized by T7 RNA polymerase. Thus, SELEX experiments with libraries carrying such modifications can lead to nuclease-resistant ligands"
Additional information: all pyrimidines have 2' amino substitutions, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1997, Nat Biotechnol ): Pagratis, N. C., Bell, C., Chang, Y. F., Jennings, S., Fitzwater, T., Jellinek, D., & Dang, C. (1997). Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nature biotechnology, 15(1), 68–73. https://doi.org/10.1038/nbt0197-68
Aptamer: 6N
Target: Keratinocyte growth factor (hKGF), Human
Type of nucleic acid: 2'-amino-RNA
Sequence: 5'GGGAGGACGAUGCGGGCAGGGAGCAAUGAACUCAAGUCAAGCCGGUGCACGUGGGCAGACGACUCGCCCGA3'
Sequence length: 71
GC content: 0.647887323943662
Affinity: Kd: 0.7 nM Ki: 26.7 nM
Pool type: 5'-GGGAGGACGATGCGG-N40-CAGACGACTCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: Ca++ and Mg++ containing Dulbeco's Phosphate Buffered Saline (DPBS, Life Technologies, Gaithersburg, MD) with 0.01% human serum albumin (Sigma, St. Louis, MO)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 26-28 kDa
Application as quoted in the referenced paper: Research: " RNA molecules with fluoro (2'F) or amino (2'NH2) substitution at the 2' position of pyrimidines are nuclease resistant' and can be synthesized by T7 RNA polymerase. Thus, SELEX experiments with libraries carrying such modifications can lead to nuclease-resistant ligands"
Additional information: all pyrimidines have 2' amino substitutions, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1997, Nat Biotechnol ): Pagratis, N. C., Bell, C., Chang, Y. F., Jennings, S., Fitzwater, T., Jellinek, D., & Dang, C. (1997). Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nature biotechnology, 15(1), 68–73. https://doi.org/10.1038/nbt0197-68
Aptamer: 2N
Target: Keratinocyte growth factor (hKGF), Human
Type of nucleic acid: 2'-amino-RNA
Sequence: 5'GGGAGGACGAUGCGGGCGGGAAGGUCCGAAGACCGGCGAAAGGAACGAGAUUGCCCAGACGACUCGCCCGA3'
Sequence length: 71
GC content: 0.6619718309859155
Affinity: Kd: 0.8 nM Ki: 66.7 nM
Pool type: 5'-GGGAGGACGATGCGG-N40-CAGACGACTCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: Ca++ and Mg++ containing Dulbeco's Phosphate Buffered Saline (DPBS, Life Technologies, Gaithersburg, MD) with 0.01% human serum albumin (Sigma, St. Louis, MO)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 26-28 kDa
Application as quoted in the referenced paper: Research: " RNA molecules with fluoro (2'F) or amino (2'NH2) substitution at the 2' position of pyrimidines are nuclease resistant' and can be synthesized by T7 RNA polymerase. Thus, SELEX experiments with libraries carrying such modifications can lead to nuclease-resistant ligands"
Additional information: all pyrimidines have 2' amino substitutions, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1997, Nat Biotechnol ): Pagratis, N. C., Bell, C., Chang, Y. F., Jennings, S., Fitzwater, T., Jellinek, D., & Dang, C. (1997). Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nature biotechnology, 15(1), 68–73. https://doi.org/10.1038/nbt0197-68
Aptamer: 24N
Target: Keratinocyte growth factor (hKGF), Human
Type of nucleic acid: 2'-amino-RNA
Sequence: 5'GGGAGGACGAUGCGGGUGGGAAGAUGAGCCGGUCGGCAGUAAUGUGACACUGCGGCAGACGACUCGCCCGA3'
Sequence length: 71
GC content: 0.647887323943662
Affinity: Kd: 1.2 nM
Pool type: 5'-GGGAGGACGATGCGG-N40-CAGACGACTCGCCCGA-3'
Pool random region: 40
Binding buffer/conditions: Ca++ and Mg++ containing Dulbeco's Phosphate Buffered Saline (DPBS, Life Technologies, Gaithersburg, MD) with 0.01% human serum albumin (Sigma, St. Louis, MO)
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 26-28 kDa
Application as quoted in the referenced paper: Research: " RNA molecules with fluoro (2'F) or amino (2'NH2) substitution at the 2' position of pyrimidines are nuclease resistant' and can be synthesized by T7 RNA polymerase. Thus, SELEX experiments with libraries carrying such modifications can lead to nuclease-resistant ligands"
Additional information: all pyrimidines have 2' amino substitutions, DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1997, Nat Biotechnol ): Pagratis, N. C., Bell, C., Chang, Y. F., Jennings, S., Fitzwater, T., Jellinek, D., & Dang, C. (1997). Potent 2'-amino-, and 2'-fluoro-2'-deoxyribonucleotide RNA inhibitors of keratinocyte growth factor. Nature biotechnology, 15(1), 68–73. https://doi.org/10.1038/nbt0197-68
Aptamer: SE RNA
Target: Monoclonal antibody 198 (Mab198)
Type of nucleic acid: 2'-amino-RNA
Sequence: 5'GGGAGAGCGGAAGCGUGCUGGGCCGGAGGUUAGCUUGCCCAUGGCAAGCAGGGCGCCACGGACCCAUAACCCAGAGGUCGAUGGAUCCCCCC3'
Sequence length: 92
GC content: 0.6739130434782609
Affinity: Kd: 60 nM
Pool type: 5'-GGGAGAGCGGAAGCGUGCUGGGCC-N40-CAUAACCCAGAGGUCGAUGGAUCCCCCC-3'
Pool random region: 40
Binding buffer/conditions: 30 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM MgCI2, 2 mM dithiothreitol, and 1% BSA
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Application as quoted in the referenced paper: Therapeutic: " Although these treatments have greatly improved medical management of the disease, the ultimate goal for therapy-the specific inhibition of the autoimmune response to AChR-has not been achieved. Therefore, because the pathogenesis of MG is largely antibody mediated, we attempted to isolate RNA decoys capable of specifically blocking antibodies that recognize the main immunogenic region of AChR"
Additional information: SE-RNA: 2' -amino SE RNA
Reference (1997, Nat Biotechnol ): Lee, S. W., & Sullenger, B. A. (1997). Isolation of a nuclease-resistant decoy RNA that can protect human acetylcholine receptors from myasthenic antibodies. Nature biotechnology, 15(1), 41–45. https://doi.org/10.1038/nbt0197-41