RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: M3
Target: 4,4′-methylenedianiline (MDA)
Type of nucleic acid: ssRNA
Sequence: 5′GCCUGUUGUGAGCCUCCUGUCGAACAGAGGCAGUACUCAUCAACCCGAGCCUUCCGUCCCUGAGUUGAGCGUUUAUUCUUGUCUCCC3′
Sequence length: 87
GC content: 0.5517241379310345
Affinity: Kd: 2 μM
Pool type: 5′-GCCTGTTGTGAGCCTCCTGTCGAA-N40-TTGAGCGTTTATTCTTGTCTCCC-3′
Pool random region: 40
Binding buffer/conditions: 250 mM NaCl/50 mM Tris–HCl/5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " The modification of cellular DNA by environmental substances is thought to be a crucial event in chemical induced carcinogenesis. Among the environmental carcinogens, aromatic amines are known for the fact that they can induce several types of cancers through the formation of so-called DNA adducts. These results open the possibility of using the SELEX technique to generate RNA molecules as diagnostic tools for the detection of DNA damaging compounds and ultimately DNA adducts."
Reference (2004, Biochem Biophys Res Commun ): Brockstedt, U., Uzarowska, A., Montpetit, A., Pfau, W., & Labuda, D. (2004). In vitro evolution of RNA aptamers recognizing carcinogenic aromatic amines. Biochemical and biophysical research communications, 313(4), 1004–1008. https://doi.org/10.1016/j.bbrc.2003.12.030
Aptamer: M22
Target: 4,4′-methylenedianiline (MDA)
Type of nucleic acid: ssRNA
Sequence: 5′GCCUGUUGUGAGCCUCCUGUCGAAUGGGAGGAGUGCUGCUCUCCAGGCACUCUUGUUGCUCGGAUUGAGCGUUUAUUCUUGUCUCCC3′
Sequence length: 87
GC content: 0.5517241379310345
Affinity: Kd: 12 μM
Pool type: 5′-GCCTGTTGTGAGCCTCCTGTCGAA-N40-TTGAGCGTTTATTCTTGTCTCCC-3′
Pool random region: 40
Binding buffer/conditions: 250 mM NaCl/50 mM Tris–HCl/5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " The modification of cellular DNA by environmental substances is thought to be a crucial event in chemical induced carcinogenesis. Among the environmental carcinogens, aromatic amines are known for the fact that they can induce several types of cancers through the formation of so-called DNA adducts. These results open the possibility of using the SELEX technique to generate RNA molecules as diagnostic tools for the detection of DNA damaging compounds and ultimately DNA adducts."
Reference (2004, Biochem Biophys Res Commun ): Brockstedt, U., Uzarowska, A., Montpetit, A., Pfau, W., & Labuda, D. (2004). In vitro evolution of RNA aptamers recognizing carcinogenic aromatic amines. Biochemical and biophysical research communications, 313(4), 1004–1008. https://doi.org/10.1016/j.bbrc.2003.12.030
Aptamer: SL-1
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCGAGCGTGCGCTTGCCTGCGTGTAGAGTCCGTCAACTCGCTCAGTGGGTGGGTGGGTGGTGAGCATGGAGTCGGATCCTCTA3′
Sequence length: 102
GC content: 0.5980392156862745
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-2
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCGCGGGGCCCGTGTGCGTTGGTCGAATTCGTGTACACTTCTCGCCTGTCTACCAGCCAGCCAGCATGGAGTCGGATCCTCTA3′
Sequence length: 102
GC content: 0.5882352941176471
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-3
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCTCGCTCGCGTGATAGAATTGAACTCACCACCTCTTGGGTAGTGTCCCGCCCGCCGAGTCCAGCATGGAGTCGGATCCTCTA3′
Sequence length: 102
GC content: 0.5686274509803921
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-4
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCTCGCTCGCGTGCTCGTCTTGAACTCACCACCTCTTGGGTAGTGTCCCGCCCGCCGAGTGGAGCATGGAGTCGGATCCTCTA3′
Sequence length: 102
GC content: 0.5980392156862745
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-5
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCGCTGCGGTAGGTGAACGGTAACGGCAAACTTCGCATGCTTGCTCGCCTGCTCGCCCCTCCAGCATGGAGTCGGATCCTCTA3′
Sequence length: 102
GC content: 0.5882352941176471
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-6
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCGCTGCGGTAGGTGAACGGTAACGGCAAACTTCGCATGCTTGCTCGCCTGCTCGCCCCTCGCAGCATGGAGTCGGATCCTCTA3′
Sequence length: 103
GC content: 0.5922330097087378
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-7
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCCCCGGGGCCAGCCACTTCCGTCAGTGAATTCCTGCTCGTATATCTACTCGCCCGCCTGCCAGCATGGAGTCGGATCCTCTA3′
Sequence length: 102
GC content: 0.5882352941176471
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-8
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCCCCGGGGCCAGCCACTTCTGTCAGTGAATTCCTGCTCGTATATCTACTCGCCCGCCTGCCAGCATGGAGTCGGATCCTCTA3′
Sequence length: 102
GC content: 0.5784313725490197
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-9
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCCCCCGGGGCCAGCCACTTCTGTCAGTGAATTCCTGCTCGTATATCTACTCGCCCGCCTGCCAGCATGGAGTCGGATCCTCTA3′
Sequence length: 103
GC content: 0.5825242718446602
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-10
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCAGCGGGGCCAGCCACTTCTGTCAGTGAATTCCTGCTCGTATATCTACTCGCCCGCCTGCCAGCATGGAGTCGGATCCTCTA3′
Sequence length: 102
GC content: 0.5686274509803921
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-11
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCAGCGGGGCCAGCCACTTCTGTCAGTGAATTCCTGCTCGTATATCTACTCGCCCGCCTGCGAGCATGGAGTCGGATCCTCTA3′
Sequence length: 102
GC content: 0.5686274509803921
Affinity: Kd: 4.9 μM
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-12
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCAGCGGGGCCAGCCACTTCTGTCAGTGAATTCCTGCTCGTATATCTACTCGCCCGCCTGGGGAGCATGGAGTCGGATCCTCTA3′
Sequence length: 103
GC content: 0.5728155339805825
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-13
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCAGCGGGGCCAGCCACTTCTGTCAGTGAATTCCTGCTCGTATATCTACTCGCCCGCCTGGGCAGCATGGAGTCGGATCCTCTA3′
Sequence length: 103
GC content: 0.5728155339805825
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-14
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCAGCGGGGCCAGCCACTTCTGTCAGTGAATTCCTGCTCGTATATCTACTCGCCCGCCTGCGGAGCATGGAGTCGGATCCTCTA3′
Sequence length: 103
GC content: 0.5728155339805825
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-15
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCCGCGCCCCCTCAGCCCTGTACGAATTCACGAGGTTGCCAGCATGGAGTCGGATCCTCTA3′
Sequence length: 80
GC content: 0.5875
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-16
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCCCGGGCCGCCCCCCTGTACGAATTCACGAGGTTGCCAGCATGGAGTCGGATCCTCTA3′
Sequence length: 78
GC content: 0.6025641025641025
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009
Aptamer: SL-17
Target: Sialyllactose
Type of nucleic acid: ssDNA
Sequence: 5′GTACGAATTCACGAGGTTGCCCAGGTCGGGGGGGGCCCCGTACGAATTCACGAGGTTGCCAGCATGGAGTCGGATCCTCTA3′
Sequence length: 81
GC content: 0.6049382716049383
Affinity: Not reported
Pool type: 5′-GTACGAATTCACGAGGTTGCC-N60-AGCATGGAGTCGGATCCTCTA-3′
Pool random region: 60
Binding buffer/conditions: Tris–HCl buffer (50 mM, pH 7.6) containing 250 mM NaCl and 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " To our knowledge, this is the first report on the selection of a modified DNA aptamer that has micromolar affinity for sialyllactose, the cell surface carbohydrate. The highaffinity sialyllactose-specific DNA aptamer might be useful for studies of cell adhesion and the development of a lead molecule for cell-adhesion-blocking anti-viral therapy."
Reference (2004, Bioorg Med Chem ): Masud, M. M., Kuwahara, M., Ozaki, H., & Sawai, H. (2004). Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX. Bioorganic & medicinal chemistry, 12(5), 1111–1120. https://doi.org/10.1016/j.bmc.2003.12.009