RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: 27
Target: α-Thrombin, Human
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAUGCCUGUCGAGCAUGCUGGUGCGGCUUUGGGCGCCGUGCUUGACGUAGCUAAACAGCUUUGUCGACGGG3'
Sequence length: 75
GC content: 0.6133333333333333
Affinity: Kd: 114 ± 2.0 nM
Pool type: 5'-CCCGTCGACAAAGCTGTTTAGCTAC-N30-CAGCATGCTCGACAGGCATCT-3'
Pool random region: 30
Binding buffer/conditions: 50 mM Tris-HCl, pH 7.7, 100 mM NaCl, 1 mM DTT, and 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.7
Molecular weight of target: Not reported
Post-SELEX modifications: Minimum sequence requirements for high affinity binding derived from clone 16 (truncated)
Application as quoted in the referenced paper: Therapeutic: " These sequences can be used as a molecular anchor to select for a larger RNA with more extensive protein interactions, including the active site. One can also envision development of a heparin mimic that could be more specific for the ATIE-mediated inhibition of thrombin. It is clear from this work and related work that oligonucleotides have great potential as antithrombotic agents."
Additional information: 5' and 3' fixed regions differ than pool non-random regions.
DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.

Reference (1994, Nucleic Acids Res ): Kubik, M. F., Stephens, A. W., Schneider, D., Marlar, R. A., & Tasset, D. (1994). High-affinity RNA ligands to human alpha-thrombin. Nucleic acids research, 22(13), 2619–2626. https://doi.org/10.1093/nar/22.13.2619
Aptamer: 16.24
Target: α-Thrombin, Human
Type of nucleic acid: ssRNA
Sequence: 5'UCCGGAUCGAAGUUAGUAGGCGGA3'
Sequence length: 24
GC content: 0.5416666666666666
Affinity: Kd: 9.3 ± 1.0 nM
Pool type: 5'-CCCGTCGACAAAGCTGTTTAGCTAC-N30-CAGCATGCTCGACAGGCATCT-3'
Pool random region: 30
Binding buffer/conditions: 50 mM Tris-HCl, pH 7.7, 100 mM NaCl, 1 mM DTT, and 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.7
Molecular weight of target: Not reported
Post-SELEX modifications: Minimum sequence requirements for high affinity binding derived from clone 16 (truncated)
Application as quoted in the referenced paper: Therapeutic: " These sequences can be used as a molecular anchor to select for a larger RNA with more extensive protein interactions, including the active site. One can also envision development of a heparin mimic that could be more specific for the ATIE-mediated inhibition of thrombin. It is clear from this work and related work that oligonucleotides have great potential as antithrombotic agents."
Additional information: DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.

Reference (1994, Nucleic Acids Res ): Kubik, M. F., Stephens, A. W., Schneider, D., Marlar, R. A., & Tasset, D. (1994). High-affinity RNA ligands to human alpha-thrombin. Nucleic acids research, 22(13), 2619–2626. https://doi.org/10.1093/nar/22.13.2619
Aptamer: 27.33
Target: α-Thrombin, Human
Type of nucleic acid: ssRNA
Sequence: 5'GAGCAUGCUGGUGCGGCUUUGGGCGCCGUGCUU3'
Sequence length: 33
GC content: 0.6666666666666666
Affinity: Kd: 155 ± 9.0 nM
Pool type: 5'-CCCGTCGACAAAGCTGTTTAGCTAC-N30-CAGCATGCTCGACAGGCATCT-3'
Pool random region: 30
Binding buffer/conditions: 50 mM Tris-HCl, pH 7.7, 100 mM NaCl, 1 mM DTT, and 1 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.7
Molecular weight of target: Not reported
Post-SELEX modifications: Minimum sequence requirements for high affinity binding derived from clone 27 (truncated)
Application as quoted in the referenced paper: Therapeutic: " These sequences can be used as a molecular anchor to select for a larger RNA with more extensive protein interactions, including the active site. One can also envision development of a heparin mimic that could be more specific for the ATIE-mediated inhibition of thrombin. It is clear from this work and related work that oligonucleotides have great potential as antithrombotic agents."
Additional information: DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.

Reference (1994, Nucleic Acids Res ): Kubik, M. F., Stephens, A. W., Schneider, D., Marlar, R. A., & Tasset, D. (1994). High-affinity RNA ligands to human alpha-thrombin. Nucleic acids research, 22(13), 2619–2626. https://doi.org/10.1093/nar/22.13.2619
Aptamer: DOPE. 40
Target: Cyanocobalamin (vitamin B12)
Type of nucleic acid: ssRNA
Sequence: 5'GUCGGCCUAUCCGACAGGCACCGCGAGAGGACCAUUAUAGUGCGCAUAACCACUUCAGUGCGAGCAAAAAUUUGG3'
Sequence length: 75
GC content: 0.5333333333333333
Affinity: Kd: 88 ± 19 nM
Pool type: Original random pool: 5'-AACACTATCCGACTG-GCACC-N72-CCTTGGTCATTAGGATCC-3' (source --> Bartel, D. P., & Szostak, J. W. (1993) Science 261,1411-1418.)
mutagenized pool( 30% dopped pool): 5'-GGAACCTCTAGGTCATTA-GGAACACTATCCGACTGGCACCGCCAGCGGACAAATCCGGTGCGCATAACCACCTCAGTGCGAGCAACGATGGCC-ACGTCAGAAGGATCCAAG-3' (inside the dash are sequence of original aptamer B12.9)
Pool random region: 72
Binding buffer/conditions: 1 M LiCl, 5 mM MgCl2, and 25 mM HEPES (pH 7.4)
Divalent salt: MgCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: DMS modification for probing the tertiary interaction in aptamer
Application as quoted in the referenced paper: Detection: " The aptamers we have selected may also serve as a starting point for the in vitro evolution of cobalamindependent ribozymes. A number of other biologically important reactions are carried out by cobalamin-dependent enzymes, including methyl group transfers, carbon skeletal rearrangements, and diol dehydrations"
Additional information: Using a doped pool for the region inside the "- -", 32P-labeled RNA was used to follow all selections.
DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
The total length of the pool RNA the paper mentioned "was 111 bases (including two new primer binding sites), with
75 mutagenized bases, and a sequence complexity of approximately 5 X 1014 molecules." However, it did not mentioed the sequence of the new primers 

Reference (1994, Biochemistry ): Lorsch, J. R., & Szostak, J. W. (1994). In vitro selection of RNA aptamers specific for cyanocobalamin. Biochemistry, 33(4), 973–982. https://doi.org/10.1021/bi00170a016
Aptamer: DOPE. 40
Target: Cobinamide dicyanide
Type of nucleic acid: ssRNA
Sequence: 5'GUCGGCCUAUCCGACAGGCACCGCGAGAGGACCAUUAUAGUGCGCAUAACCACUUCAGUGCGAGCAAAAAUUUGG3'
Sequence length: 75
GC content: 0.5333333333333333
Affinity: Kd: 20 ± 9 uM
Pool type: Original random pool: 5'-AACACTATCCGACTG-GCACC-N72-CCTTGGTCATTAGGATCC-3' (source --> Bartel, D. P., & Szostak, J. W. (1993) Science 261,1411-1418.)
mutagenized pool( 30% dopped pool): 5'-GGAACCTCTAGGTCATTA-GGAACACTATCCGACTGGCACCGCCAGCGGACAAATCCGGTGCGCATAACCACCTCAGTGCGAGCAACGATGGCC-ACGTCAGAAGGATCCAAG-3' (inside the dash are sequence of original aptamer B12.9)
Pool random region: 72
Binding buffer/conditions: 1 M LiCl, 5 mM MgCl2, and 25 mM HEPES (pH 7.4)
Divalent salt: MgCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: DMS modification for probing the tertiary interaction in aptamer
Application as quoted in the referenced paper: Detection: " The aptamers we have selected may also serve as a starting point for the in vitro evolution of cobalamindependent ribozymes. A number of other biologically important reactions are carried out by cobalamin-dependent enzymes, including methyl group transfers, carbon skeletal rearrangements, and diol dehydrations"
Additional information: Using a doped pool for the region inside the "- -", 32P-labeled RNA was used to follow all selections.
DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.

Reference (1994, Biochemistry ): Lorsch, J. R., & Szostak, J. W. (1994). In vitro selection of RNA aptamers specific for cyanocobalamin. Biochemistry, 33(4), 973–982. https://doi.org/10.1021/bi00170a016
Aptamer: B12.9
Target: Cobinamide dicyanide
Type of nucleic acid: ssRNA
Sequence: 5'AACACUAUCCGACUGGCACCGCCAGCGGACAAAUCCGGUGCGCAUAACCACCUCAGUGCGAGCAACGAUGGCCUUUCUACCCAAAGAUUUUCCUUGGUCAUUAGGAUCC3'
Sequence length: 109
GC content: 0.5321100917431193
Affinity: Kd: 8.8 ± 0.5 uM
Pool type: Original random pool 5'-AACACTATCCGACTG-GCACC-N72-CCTTGGTCATTAGGATCC-3' (source --> Bartel, D. P., & Szostak, J. W. (1993) Science 261,1411-1418.)
Pool random region: 72
Binding buffer/conditions: 1 M LiCl, 5 mM MgCl2, and 25 mM HEPES (pH 7.4)
Divalent salt: MgCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: DMS modification for probing the tertiary interaction in aptamer
Application as quoted in the referenced paper: Detection: " The aptamers we have selected may also serve as a starting point for the in vitro evolution of cobalamindependent ribozymes. A number of other biologically important reactions are carried out by cobalamin-dependent enzymes, including methyl group transfers, carbon skeletal rearrangements, and diol dehydrations"
Additional information: DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.

Reference (1994, Biochemistry ): Lorsch, J. R., & Szostak, J. W. (1994). In vitro selection of RNA aptamers specific for cyanocobalamin. Biochemistry, 33(4), 973–982. https://doi.org/10.1021/bi00170a016
Aptamer: B12.9
Target: Cyanocobalamin (vitamin B12)
Type of nucleic acid: ssRNA
Sequence: 5'AACACUAUCCGACUGGCACCGCCAGCGGACAAAUCCGGUGCGCAUAACCACCUCAGUGCGAGCAACGAUGGCCUUUCUACCCAAAGAUUUUCCUUGGUCAUUAGGAUCC3'
Sequence length: 109
GC content: 0.5321100917431193
Affinity: Kd: 320 ± 90 nM
Pool type: Original random pool 5'-AACACTATCCGACTG-GCACC-N72-CCTTGGTCATTAGGATCC-3' (source --> Bartel, D. P., & Szostak, J. W. (1993) Science 261,1411-1418.)
Pool random region: 72
Binding buffer/conditions: 1 M LiCl, 5 mM MgCl2, and 25 mM HEPES (pH 7.4)
Divalent salt: MgCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: DMS modification for probing the tertiary interaction in aptamer
Application as quoted in the referenced paper: Detection: " The aptamers we have selected may also serve as a starting point for the in vitro evolution of cobalamindependent ribozymes. A number of other biologically important reactions are carried out by cobalamin-dependent enzymes, including methyl group transfers, carbon skeletal rearrangements, and diol dehydrations"
Additional information: DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.

Reference (1994, Biochemistry ): Lorsch, J. R., & Szostak, J. W. (1994). In vitro selection of RNA aptamers specific for cyanocobalamin. Biochemistry, 33(4), 973–982. https://doi.org/10.1021/bi00170a016
Aptamer: 35-mer aptamer
Target: Cyanocobalamin (vitamin B12)
Type of nucleic acid: ssRNA
Sequence: 5'GGAACCGGUGCGCAUAACCACCUCAGUGCGAGCAA3'
Sequence length: 35
GC content: 0.6
Affinity: Kd: 88 ± 19 nM
Pool type: Original random pool 5'-AACACTATCCGACTG-GCACC-N72-CCTTGGTCATTAGGATCC-3' (source --> Bartel, D. P., & Szostak, J. W. (1993) Science 261,1411-1418.)
Pool random region: 72
Binding buffer/conditions: 1 M LiCl, 5 mM MgCl2, and 25 mM HEPES (pH 7.4)
Divalent salt: MgCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: DMS modification for probing the tertiary interaction in aptamer
Based on the sequence data from the mutagenized pool selection, a smaller aptamer (35 nucleotides long) was made by run-off transcription of a synthetic DNA oligonucleotide (Truncation/minimal sequence)
Application as quoted in the referenced paper: Detection: " The aptamers we have selected may also serve as a starting point for the in vitro evolution of cobalamindependent ribozymes. A number of other biologically important reactions are carried out by cobalamin-dependent enzymes, including methyl group transfers, carbon skeletal rearrangements, and diol dehydrations"
Additional information: DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.

Reference (1994, Biochemistry ): Lorsch, J. R., & Szostak, J. W. (1994). In vitro selection of RNA aptamers specific for cyanocobalamin. Biochemistry, 33(4), 973–982. https://doi.org/10.1021/bi00170a016
Aptamer: 35-mer aptamer
Target: Cobinamide dicyanide
Type of nucleic acid: ssRNA
Sequence: 5'GGAACCGGUGCGCAUAACCACCUCAGUGCGAGCAA3'
Sequence length: 35
GC content: 0.6
Affinity: Kd: 19.7 ± 8.7 uM
Pool type: Original random pool 5'-AACACTATCCGACTG-GCACC-N72-CCTTGGTCATTAGGATCC-3' (source --> Bartel, D. P., & Szostak, J. W. (1993) Science 261,1411-1418.)
Pool random region: 72
Binding buffer/conditions: 1 M LiCl, 5 mM MgCl2, and 25 mM HEPES (pH 7.4)
Divalent salt: MgCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: DMS modification for probing the tertiary interaction in aptamer
Based on the sequence data from the mutagenized pool selection, a smaller aptamer (35 nucleotides long) was made by run-off transcription of a synthetic DNA oligonucleotide (Truncation/minimal sequence)
Application as quoted in the referenced paper: Detection: " The aptamers we have selected may also serve as a starting point for the in vitro evolution of cobalamindependent ribozymes. A number of other biologically important reactions are carried out by cobalamin-dependent enzymes, including methyl group transfers, carbon skeletal rearrangements, and diol dehydrations"
Additional information: DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.

Reference (1994, Biochemistry ): Lorsch, J. R., & Szostak, J. W. (1994). In vitro selection of RNA aptamers specific for cyanocobalamin. Biochemistry, 33(4), 973–982. https://doi.org/10.1021/bi00170a016
Aptamer: Clone 3
Target: Thrombin, Human
Type of nucleic acid: 5-uracil-modified-DNA
Sequence: 5'TAGAATACTCAAGCTTCGACGCAGAXACAGGCCAXGXGCAGTTTGGATCCCCGGGTAC3'
Sequence length: 55
GC content: 0.5454545454545454
Affinity: Kd: 800 nM
Pool type: 5'-TAGAATACTCAAGCTTCGACG-N20-AGTTTGGATCCCCGGGTAC-3'
Pool random region: 20
Binding buffer/conditions: 20 mM Tris—acetate pH 7.4, 140 mM NaCl, 5 mM KC1, 1 mM MgCl2, 1 mM CaCl2
Divalent salt: MgCl/CaCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " We demonstrate that the incorporation of this hydrophobic group into the random oligonucleotide library signficantly alters the outcome of the selection process against human thrombin. The isolated aptamers display enrichment for molecules containing extensive substitution with this modified nucleotide. Binding and anticoagulant activity of the isolated aptamers are discussed."
Additional information: X denotes pentynyl dU as determined via sequencing. 5-(1-pentynyl)-2'-deoxyuridine used instead of thymidine ("X" in sequences) it is also called 5-pentynyl-dU
Reference (1994, Nucleic Acids Res ): Latham, J. A., Johnson, R., & Toole, J. J. (1994). The application of a modified nucleotide in aptamer selection: novel thrombin aptamers containing 5-(1-pentynyl)-2'-deoxyuridine. Nucleic acids research, 22(14), 2817–2822. https://doi.org/10.1093/nar/22.14.2817
Aptamer: mTCT8-4
Target: Bronchodilator theophylline
Type of nucleic acid: ssRNA
Sequence: 5'AGUGAUACCAGCAUCGUCUUGAUGCCCUUGGCAGCACU3'
Sequence length: 38
GC content: 0.5263157894736842
Affinity: Kd: 0.1 μM
Pool type: Not reported (N40)
Pool random region: 40
Binding buffer/conditions: Sepharose column conditions: 60 mg of N-hydroxysuccinimide and 190 mg of 1 -ethyl-3,3-dimethylaminopropyl carbodiimide in a 5-mi 1:1 mixture of dioxane and phosphatebuffered saline (pH 7.2)
Buffer type: PBS/phosphate buffers
pH: 7.2
Molecular weight of target: Not reported
Post-SELEX modifications: 38-nucleotide truncated version of the TCT8-4 aptamer. 
Application as quoted in the referenced paper: Diagnostic: " One of the selected RNAs shows binding discrimination between theophylline and caffeine that is 10-fold better than that for available antibodies. In addition, this RNA possesses a binding affinity to theophylline over 100-fold greater than that to other oligonucleotides that have been selected to bind to small molecule targets. These results illustrate that small RNAs can display molecular recognition and specificity with extremely high resolution and illustrate the potential utility of oligonucleotides as diagnostic reagents."
Additional information: Alternative sequence report: (Nx)AUACCA(Nx)CCUUGG(C/A)AG(Nx)
Reference (1994, Science ): Jenison, R. D., Gill, S. C., Pardi, A., & Polisky, B. (1994). High-resolution molecular discrimination by RNA. Science (New York, N.Y.), 263(5152), 1425–1429. https://doi.org/10.1126/science.7510417
Aptamer: TCT8-4
Target: Bronchodilator theophylline
Type of nucleic acid: ssRNA
Sequence: 5'AAGUGAUACCAGCAUCGUCUUGAUGCCCUUGGCAGCACUUCA3'
Sequence length: 42
GC content: 0.5
Affinity: Kd: 0.6 μM
Pool type: Not reported (N40)
Pool random region: 40
Binding buffer/conditions: Sepharose column conditions: 60 mg of N-hydroxysuccinimide and 190 mg of 1 -ethyl-3,3-dimethylaminopropyl carbodiimide in a 5-mi 1:1 mixture of dioxane and phosphatebuffered saline (pH 7.2)
Buffer type: PBS/phosphate buffers
pH: 7.2
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " One of the selected RNAs shows binding discrimination between theophylline and caffeine that is 10-fold better than that for available antibodies. In addition, this RNA possesses a binding affinity to theophylline over 100-fold greater than that to other oligonucleotides that have been selected to bind to small molecule targets. These results illustrate that small RNAs can display molecular recognition and specificity with extremely high resolution and illustrate the potential utility of oligonucleotides as diagnostic reagents."
Additional information: Alternative sequence report: (Nx)AUACCA(Nx)CCUUGG(C/A)AG(Nx)
Reference (1994, Science ): Jenison, R. D., Gill, S. C., Pardi, A., & Polisky, B. (1994). High-resolution molecular discrimination by RNA. Science (New York, N.Y.), 263(5152), 1425–1429. https://doi.org/10.1126/science.7510417
Aptamer: 44.Cit11
Target: L-citrulline [L-(+)-2-amino-5-ureidovaleric acid]
Type of nucleic acid: ssRNA
Sequence: 5'GACGAGAAGGAGUGCUGGUUAUACUAGCGGUUAGGUCACUCGUC3'
   
Sequence length: 44
GC content: 0.5227272727272727
Affinity: Kd: 62-68 µM
Pool type: 5'-GGAGCTCAGCCTTCACTGC-N74-GGCACCACGGTCGGATCC-3'
Pool random region: 74
Binding buffer/conditions: 250 mM NaCl; 50 mM Tris-HCl, pH = 7.6; 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: On the basis of the similarities of the individual sequences, a 44-mer RNA was constructed
Application as quoted in the referenced paper: Research: " We now report the isolation of RNAs able to bind the amino acid L-tyrosine+ The tyrosine aptamers were obtained by in vitro evolution of a previously selected dopamine aptamer. Tyrosine-binding sites are characterized by the presence of both tyrosine (UAU and UAC) and termination (UAG and UAA) triplets."
Additional information: extended descriptions of the aptamer structures can be found here: https://doi.org/10.1093/nar/23.23.4769

DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.

Reference (1994,RNA): Famulok, M. (1994). Molecular recognition of amino acids by RNA-aptamers: An L-citrulline binding RNA motif and its evolution into an L-arginine binder. Journal of the American Chemical Society, 116(5), 1698–1706. https://doi.org/10.1021/ja00084a010
Aptamer: 44.Arg11
Target: L-arginine
Type of nucleic acid: ssRNA
Sequence: 5'GACGAGAAGGAGCGCUGGUUCUACUAGCAGGUAGGUCACUCGUC3'
Sequence length: 44
GC content: 0.5681818181818182
Affinity: Kd: 56-76 µM
Pool type: 5'-GGAGCTCAGCCTTCACTGC-N74-GGCACCACGGTCGGATCC-3'
Pool random region: 74
Binding buffer/conditions: 250 mM NaCl; 50 mM Tris-HCl, pH = 7.6; 5 mM MgCl2
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.6
Molecular weight of target: Not reported
Post-SELEX modifications: On the basis of the similarities of the individual sequences, a 44-mer RNA was constructed
Application as quoted in the referenced paper: Research: " We now report the isolation of RNAs able to bind the amino acid L-tyrosine+ The tyrosine aptamers were obtained by in vitro evolution of a previously selected dopamine aptamer. Tyrosine-binding sites are characterized by the presence of both tyrosine (UAU and UAC) and termination (UAG and UAA) triplets."
Additional information: DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.

Reference (1994,RNA): Famulok, M. (1994). Molecular Recognition of Amino Acids by RNA-Aptamers: An L-Citrulline Binding RNA Motif and Its Evolution into an L-Arginine Binder. Journal of the American Chemical Society, 116(5), 1698–1706. https://doi.org/10.1021/ja00084a010
Aptamer: Clone 6
Target: Protein kinase C beta II (protein kinase C βII)
Type of nucleic acid: ssRNA
Sequence: 5'GGGAGAAUUCCGACCAGAGGCUUACAGAGUGUGCGUAAUGGCGUUCCCAAAUUCGGGCUGGGAACCGUUCGUUCGUGUUAUGCCCGUAGAUAUGGCAAGUCGCGGAUGCUCAGUACUACACUCUUGUGGUCAGUCACAUAUGUGCGUCUACAUGGAUCCUCA3'
Sequence length: 162
GC content: 0.5185185185185185
Affinity: Kd: 7 nM
Pool type: 5'-GGGAGAAUUCCGACCAGAGGCUU-N120-CAUAUGUGCGUCUACAUGGAUCCUCA-3'
Pool random region: 120
Binding buffer/conditions: 20 mM HEPES, pH 7.5, 10 mM MgCl,, 0.3 m~ CaCl,, 1 mM DTT, 0.05 m~ ATP
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " This work opens the possibility that the substrate recognition properties conferred on most proteins by natural selection can be mimicked by artificial evolution and that new allosteric inhibitors and activators of enzymes may be found using in vitro selection. As a practical example, expression of these aptamers in cells should now allow inhibition of one specific protein kinase C isozyme, thus opening the possibility for dissecting the roles of protein kinase C isozymes in signal transduction."
Additional information: Paper says RNA aptamers but reports the sequences in DNA so T's were changed to U's in the spreadsheet
DNA library/pool was used as a template to generate the RNA pool used in the selection. A T7 promoter sequence might be necessary to use this DNA library/pool as a template to generate the RNA pool in the selection.
Reference (1994, J Biol Chem ): Conrad, R., Keranen, L. M., Ellington, A. D., & Newton, A. C. (1994). Isozyme-specific inhibition of protein kinase C by RNA aptamers. The Journal of biological chemistry, 269(51), 32051–32054.