RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: 4.4.6
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGCTTCATTTCTTCCGAGCTGTGACGACCGTATTTTCCTACGCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.5125
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.8
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGTGTATTGAGTCACTGGTTTTAACTTTTCGTGGGGCCGGTTCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.5
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.9
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGTTTAGCCCGCTCAGTCATCTGTAACTCGTCACCTCATTTGCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.5125
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.10
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGCTATGAGCAGATCCGATAGAAAGTTAATGCCCTAGAACATCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.475
Affinity: Kd: 33 ± 36 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.11
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGAACTCACAAACTAGTTTTTGGTGTCGTTGTGCTCCGTTGTCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.4875
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.12
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGTGAAACATAGCATATTTACTTATGTCGCCTTGCCGGTTCCTATGCGTGCTACCGTGAA3′
Sequence length: 78
GC content: 0.4743589743589743
Affinity: Kd: 23 ± 12 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.13
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGTTCATCATTGTATCCTGGGTTCATGTGAGCACAAAGGTGTCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.4875
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.15
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGCTCCAAAGCGGGCGACTCTCAGGAACGCGGCTCAATTACCCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.575
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.16
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGAAATTTTGCACTTACTTGACGTTTCCTCGATAGCCACAAGCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.475
Affinity: Kd: 39 ± 18 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.17
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGTGCTTATTTAGTCCCATGGTCAATTGCTGCGTATATAGTGCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.475
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.18
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGTGCGATAAATAAATATTACACTGCGAATTTAGTAAAGGTGCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.425
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.19
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGTAGGACCTCAATACACAGCTGTGTTTATGAATAATATCCCCTATGCGTGCTACCGTGAA3′
Sequence length: 79
GC content: 0.4556962025316455
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.20
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGTGGTTCGATGCTGCAAGGTGTTGTCATTTGGCATATGTTACCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.4875
Affinity: Kd: 25 ± 20 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.21
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGACGATCTCAGGTGATTAGCCGAGCTGTGTTCGAAACTAACCTATGCGTGCTACCGTGAA3′
Sequence length: 79
GC content: 0.5063291139240507
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.22
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGGTCCAGACGATGCGCTCGATATAGAGGCATTCATTTCGGACCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.525
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.23
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGATGTGTGAGGTTCAAAAACGTGCCCACGTTAAAGACCGGTCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.5125
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.24
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGTACGGGAACCATCCGTGGTGAGGTGAACTACATACTGCCGCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.55
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.25
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGTGGGGTTTTGGTAGCCTTTGCAATCCTTATGCGTCTGAGCCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.525
Affinity: Not reported
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: 4.4.27
Target: Immunoglobulin E (IgE), Human
Type of nucleic acid: ssDNA
Sequence: 5′AGCAGCACAGAGGTCAGATGAGATGGTTGACCGGTTGATGTGATTAAGGAGACGTCGATTCCTATGCGTGCTACCGTGAA3′
Sequence length: 80
GC content: 0.5
Affinity: Kd: 23 ± 13 nM
Pool type: 5′-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3′
Pool random region: 40
Binding buffer/conditions: 10 mM Tris, 50 mM NaCl, and 1 mM EDTA at pH 7.5
Buffer type: Tris Buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Aptamers with high affinity for IgE were selected using capillary electrophoresis to demonstrate the compatibility of this technique with SELEX. The high selectivity and efficiency of CE gave rise to a very high rate of enrichment, allowing high-affinity, high-selectivity aptamers to be obtained in only four rounds of selection."
Reference (2004, Anal Chem ): Mendonsa, S. D., & Bowser, M. T. (2004). In vitro selection of high-affinity DNA ligands for human IgE using capillary electrophoresis. Analytical chemistry, 76(18), 5387–5392. https://doi.org/10.1021/ac049857v
Aptamer: aptamer 1
Target: Anti-MUC1 IgG3 monoclonal antibody; anti-MUC1 IgG3 MAb, C595 MAb (epitope: Arg-Pro-Ala-Pro)
Type of nucleic acid: ssDNA
Sequence: 5′GAACTGAGGTGCTTTCCCAAAACCT3′
Sequence length: 25
GC content: 0.48
Affinity: Kd: 50 nM
Pool type: 5′-GGGAGACAAGAATAAACGCTCAA-N25-TTCGACAGGAGGCTCACAACAGGC-′3.
Pool random region: 25
Binding buffer/conditions: Carbonate buffer (pH 9.6) or PBS (pH 7.4)
Buffer type: PBS/phosphate buffers
pH: 9.6
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic and Diagnostic: "The ease of selection of aptamers against the C595 antibody and its binding characteristics make it possible for aptamers to be rapidly generated using the proposed selection methodology and used as antibody-targeting entities that could be used in a number of applications, ranging from antibody purification to immunoassays and vaccine applications. Finally, their labelling potential may allow their use in imaging and as anti-idiotypic targeted radiopharmaceuticals, with potentially numerous therapeutic applications in disease diagnosis, imaging and therapy."
Reference (2004, J Immunol Methods ): Missailidis, S., Thomaidou, D., Borbas, K. E., & Price, M. R. (2005). Selection of aptamers with high affinity and high specificity against C595, an anti-MUC1 IgG3 monoclonal antibody, for antibody targeting. Journal of immunological methods, 296(1-2), 45–62. https://doi.org/10.1016/j.jim.2004.10.011