RosettaCommons/UTexasAptamer huggingface dataset:
Aptamer: CCFM641-5
Target: Bifidobacterium bifidum (B. bifidum)
Type of nucleic acid: ssDNA
Sequence: 5'AGCAGCACAGAGGTCAGATGTGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGCCCTATGCGTGCTACCGTGAA3'
Sequence length: 80
GC content: 0.6125
Affinity: Kd: 10.69 ± 0.89 nM
Pool type: 5'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'
Pool random region: 40
Binding buffer/conditions: Phosphate-buffere saline (PBS, pH 7.2)
Buffer type: PBS/phosphate buffers
pH: 7.2
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " A whole-bacterium-based SELEX (Systematic Evolution of Ligands by Exponential Enrichment) procedure was adopted in this study for the selection of an ssDNA aptamer that binds to Bifidobacterium bifidum. To prove the potential application of the aptamer CCFM641-5, a colorimetric bioassay of the sandwich-type structure was used to detect B. bifidum. The assay had a linear range of 10⁴ to 10⁷ cfu/mL (R² = 0.9834). Therefore, the colorimetric bioassay appears to be a promising method for the detection of B. bifidum based on the aptamer CCFM641-5."
Additional information: utilized whole-bacterium-based SELEX
Reference (2017, Int J Mol Sci ): Hu, L., Wang, L., Lu, W., Zhao, J., Zhang, H., & Chen, W. (2017). Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidum. International journal of molecular sciences, 18(5), 883. https://doi.org/10.3390/ijms18050883
Aptamer: RAC-6
Target: Ractopamine (RAC)
Type of nucleic acid: ssDNA
Sequence: 5'AGCAGCACAGAGGTCAGATGGTCTCTACTAAAAGTTTTGATCATACCGTTCACTAATTGACCTATGCGTGCTACCGTGAA3'
Sequence length: 80
GC content: 0.4375
Affinity: Kd: 54.22 ± 8.02 nM
Pool type: 5'-AGCAGCACAGAGGTCAGATG-N40-CCTATGCGTGCTACCGTGAA-3'
Pool random region: 40
Binding buffer/conditions: 50 mM Tris-HCl, 5 mM KCl, 100 mM NaCl, 1 mM MgCl2, pH 7.4
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Research and detection: " An improved SELEX technique was developed for selecting aptamers against ractopamine (RAC) by immobilizing ssDNA library on the magnetic beads. One representative aptamer candidate from each family was picked out for binding affinity identification by graphene oxide (GO) adsorption platform. The aptamer RAC-6 was demonstrated as the optimal aptamer with high specificity and dissociation constant. To prove the potential application of aptamer RAC-6 in the quantitative determination of RAC, a fluorescent bioassay with aptamer RAC-6 was developed."
Reference (2017, Anal Chim Acta ): Duan, N., Gong, W., Wu, S., & Wang, Z. (2017). An ssDNA library immobilized SELEX technique for selection of an aptamer against ractopamine. Analytica chimica acta, 961, 100–105. https://doi.org/10.1016/j.aca.2017.01.008
Aptamer: AM-6
Target: Escherichia coli (E. coli) O157:H7
Type of nucleic acid: ssDNA
Sequence: 5'CGTGATGATGTTGAGTTGGGGTGATGGGTGCATGTGATGAAAGGGGTTCGTGCTATGCTGTTTTGTCTAATAATACTAGTCCTTGCCAAGGTTTATTCCAGTAATGCCAACCAATCT3'
Sequence length: 117
GC content: 0.4358974358974359
Affinity: Kd: 107.6 ± 67.8 pM
Pool type: 5'-CGTGATGATGTTGAGTTG-N80-CAGTAATGCCAACCAATCT-3'
Pool random region: 80
Binding buffer/conditions: 100 mM NaCl, 1 mM MgCl2, 50 mM Tris-HCl, 5 mM KCl
Divalent salt: MgCl
Buffer type: Tris Buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " In this study, systematic evolution of ligands by exponential enrichment using whole cells (Cell-SELEX) method was used for recognizing E. coli strain, O157 by single-stranded DNA library of aptamer. The isolated aptamer efficiency was confirmed and it was shown that the new DNA aptamer sequence has the ability to use for detection. This specific O157:H7 aptamer have the potential for application as a diagnostic ligand and could be used for detection of the related food borne diseases."
Reference (2017, Anal Biochem ): Amraee, M., Oloomi, M., Yavari, A., & Bouzari, S. (2017). DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX method. Analytical biochemistry, 536, 36–44. https://doi.org/10.1016/j.ab.2017.08.005
Aptamer: GR-3
Target: Glucagon receptor (GCGR)
Type of nucleic acid: ssDNA
Sequence: 5'ATCCAGAGTGACGCAGCAGATAAGTAGGTATCCGTTTGAAAAACTTTTCTGACCGTCCGACTATGGACACGGTGGCTTAGT3'
Sequence length: 81
GC content: 0.4691358024691358
Affinity: Kd: 52.7 ± 5.1 nM
Pool type: 5′-ATCCAGAGTGACGCAGCA-N45-TGGACACGGTGGCTTAGT-3′
Pool random region: 45
Binding buffer/conditions: 5 mM MgCl2 and 4.5 g/L glucose in Dulbecco's Phosphate Buffered Saline (D-PBS, pH = 7.4) and 0.1 mg/mL yeast tRNA and 1 mg/L bovine serum albumin (BSA)
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " Excessive secretion of glucagon, a functional insulin antagonist, significantly contributes to hyperglycemia. Glucagon exerts its physiological functions through activation of the glucagon receptor (GCGR). Here, we have successfully selected a DNA aptamer against GCGR by cell-SELEX, which can specifically bind membrane protein of CHO-GCGR cells. Hepatic tissue imaging suggested that GR-3 could bind the cell membrane of hepatic tissues. With the advantages of small size, high binding affinity, good stability, lack of immunogenicity, and easy synthesis, aptamer GR-3 against GCGR can be a promising tool with the potential to attenuate hyperglycemia in diabetes mellitus."
Reference (2017, Sci Rep ): Wang, G., Liu, J., Chen, K., Xu, Y., Liu, B., Liao, J., Zhu, L., Hu, X., Li, J., Pu, Y., Zhong, W., Fu, T., Liu, H., & Tan, W. (2017). Selection and characterization of DNA aptamer against glucagon receptor by cell-SELEX. Scientific reports, 7(1), 7179. https://doi.org/10.1038/s41598-017-05840-w
Aptamer: C10K2
Target: Osteoponin (OPN), Human
Type of nucleic acid: ssDNA
Sequence: 5'GGGGGTGGTACCAGAGATGCTGTGTGCGGCACTCCAGTCTGTTACGCCGCCAGAGAGGAGGTACCGTGGG3'
Sequence length: 70
GC content: 0.6428571428571429
Affinity: Kd: <2.5 nM
Pool type: 5'-GGGGGTGGTACCAGAGATGC-N30-CAGAGAGGAGGTACCGTGGG-3'
Pool random region: 30
Binding buffer/conditions: Phosphate buffer saline (PBS) solution (137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4 and 1.47 mM KH2PO4) with an adjusted pH 7.6
Buffer type: PBS/phosphate buffers
pH: 7.6
Molecular weight of target: Between 41 and 75 kDa
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Detection: " A DNA aptamer with affinity and specificity for human osteopontin (OPN), a potential breast cancer biomarker, was selected using the SELEX process, considering its homology rate and the stability of its secondary structures. Our aptasensor coupled with SWV represents a promising alternative for the detection of relevant breast cancer biomarkers."
Reference (2017, Anal Chim Acta ): Meirinho, S. G., Dias, L. G., Peres, A. M., & Rodrigues, L. R. (2017). Electrochemical aptasensor for human osteopontin detection using a DNA aptamer selected by SELEX. Analytica chimica acta, 987, 25–37. https://doi.org/10.1016/j.aca.2017.07.071
Aptamer: BBA1
Target: Benzylpenicillin (penicillin G)
Type of nucleic acid: ssDNA
Sequence: 5′ATGCGGATCCCGCGCGGGTCTGAGGAGTGCGCGGTGCCAGTGAGTGCGCGAAGCTTGCGC3'
Sequence length: 60
GC content: 0.7
Affinity: Kd: 383.4 nM
Pool type: 5′-ATGCGGATCCCGCGC-N30-GCGCGAAGCTTGCGC-3
Pool random region: 30
Binding buffer/conditions: 1x binding buffer (20 mM Tris) and 2x binding buffer (40 mM Tris)
Buffer type: Tris Buffers
Molecular weight of target:  not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Dectection: " Antibiotics are useful for improving the living conditions of livestock. However, residual antibiotics induce several human diseases such as food-borne illness and infection of carbapenem-resistant Enterobacteriaceae (CRE). In this study, the identification of a benzylpenicillin-specific aptamer was selected by rGO-SELEX (reduced Graphene Oxide-Systematic Evolution of Ligands by EXponential enrichment). With these advantageous characteristics, we expect that this aptamer could be applied to an on-site detection system for residual benzylpenicillin."
Reference (2017, Anal Biochem ): Lee, A. Y., Ha, N. R., Jung, I. P., Kim, S. H., Kim, A. R., & Yoon, M. Y. (2017). Development of a ssDNA aptamer for detection of residual benzylpenicillin. Analytical biochemistry, 531, 1–7. https://doi.org/10.1016/j.ab.2017.05.013
Aptamer: J3
Target: Metastatic colorectal carcinoma cell line LoVo
Type of nucleic acid: ssDNA
Sequence: 5'CCTGAACCTGATGCCAACCTGCCAGCGGGCAGTGCGCGAGTGGGAAACCGAGGGGGACTGAGTAGCGAGCGTGTAGTGTG3'
Sequence length: 80
GC content: 0.6375
Affinity: Kd: 138.2±100.1 nM
Pool type: 5′-CCTGAACCTGATGCCAACCT-N40-AGTAGCGAGCGTGTAGTGTG-3′
Pool random region: 40
Binding buffer/conditions: Binding buffer (4.5g/L glucose, 5 mM MgCl2, 1 mg/mL BSA and 0.1 mg/mL yeast tRNA in 0.01 M PBS, pH 7.4)
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target:  not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " Early diagnosis of cancer metastasis may significantly improve cure rate of patients who are at high risk for developing metastasis. In this study, we successfully achieved metastatic cancer cell and tissue-specific fluorescence imaging by using a new aptamer developed by cell-based systematic evolution of ligands by exponential enrichment (Cell-SELEX). The targeting reagent J3-based fluorescence imaging possesses great potential for clinical diagnosis of cancer metastasis."
Reference (2017, Talanta ): Yuan, B., Jiang, X., Chen, Y., Guo, Q., Wang, K., Meng, X., Huang, Z., & Wen, X. (2017). Metastatic cancer cell and tissue-specific fluorescence imaging using a new DNA aptamer developed by Cell-SELEX. Talanta, 170, 56–62. https://doi.org/10.1016/j.talanta.2017.03.094
Aptamer: Crn-1
Target: Salmonella enteritidis (S. enteritidis)
Type of nucleic acid: ssDNA
Sequence: 5'AAGGGCTGGCTGGGATGGACCCTCCCGAAACGAGCTGTCTCTTAACGGAAGCTAATCTGCCTCACTCCACGGACCCCACT3'
Sequence length: 80
GC content: 0.5875
Affinity: Kd: 971 nM
Pool type: 5′-AAGGGCTGGCTGGGATGGA-N42-TCACTCCACGGACCCCACT-3′
Pool random region: 42
Binding buffer/conditions: Binding buffer (4.5 g/L glucose, 5mM MgCl2, 0.1mg/mL yeast tRNA, and 1mg/mL BSA in Dulbecco's PBS
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target:  not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Salmonella Enteritidis is most widely found bacteria causing food borne diseases. Therefore, simple, rapid, and specific detection methods are needed for food safety. In this study, we demonstrated the selection of DNA aptamers with high affinity and specificity against S. Enteritidis via Cell Systematic Evolution of Ligands by Exponential Enrichment (Cell-SELEX). This platform is also suitable for detection of S. Enteritidis in complex food matrix. Thus, this is the first to demonstrate use of Salmonella aptamers for development of the colorimetric aptamer-based detection platform in its identification and detection with naked eye in point-of-care."
Reference (2017, Biosens Bioelectron ): Bayraç, C., Eyidoğan, F., & Avni Öktem, H. (2017). DNA aptamer-based colorimetric detection platform for Salmonella Enteritidis. Biosensors & bioelectronics, 98, 22–28. https://doi.org/10.1016/j.bios.2017.06.029
Aptamer: Crn-2
Target: Salmonella enteritidis (S. enteritidis)
Type of nucleic acid: ssDNA
Sequence: 5'AAGGGCTGGCTGGGATGGATGTAAGAAGGGAGGAAAGGACCTAAGACCTGCTATATTGCGATCACTCCACGGACCCCACT3'
Sequence length: 80
GC content: 0.5375
Affinity: Kd: 309 nM
Pool type: 5′-AAGGGCTGGCTGGGATGGA-N42-TCACTCCACGGACCCCACT-3′
Pool random region: 42
Binding buffer/conditions: Binding buffer (4.5 g/L glucose, 5mM MgCl2, 0.1mg/mL yeast tRNA, and 1mg/mL BSA in Dulbecco's PBS
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Salmonella Enteritidis is most widely found bacteria causing food borne diseases. Therefore, simple, rapid, and specific detection methods are needed for food safety. In this study, we demonstrated the selection of DNA aptamers with high affinity and specificity against S. Enteritidis via Cell Systematic Evolution of Ligands by Exponential Enrichment (Cell-SELEX). This platform is also suitable for detection of S. Enteritidis in complex food matrix. Thus, this is the first to demonstrate use of Salmonella aptamers for development of the colorimetric aptamer-based detection platform in its identification and detection with naked eye in point-of-care."
Reference (2017, Biosens Bioelectron ): Bayraç, C., Eyidoğan, F., & Avni Öktem, H. (2017). DNA aptamer-based colorimetric detection platform for Salmonella Enteritidis. Biosensors & bioelectronics, 98, 22–28. https://doi.org/10.1016/j.bios.2017.06.029
Aptamer: SS-3
Target: Shigella sonnei (S. sonnei)
Type of nucleic acid: ssDNA
Sequence: 5'ATACCAGCTTATTCAATTCCATGGTCCCTCGTGTTTATTATGTTGTCTGAACTGGCTGAGATTGCACTTACTATCT3'
Sequence length: 76
GC content: 0.3947368421052631
Affinity: Kd: 39.32 ± 5.02 nM
Pool type: 5′-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3′
Pool random region: 40
Binding buffer/conditions: TBS buffer (10 mM Tris-HCl, 0.85% NaCl, pH 8.0)
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " In this paper, a Whole-Bacteria SELEX (WB-SELEX) strategy was adopted to isolate specific aptamers against Shigella sonnei. Real-time PCR amplification and post-SELEX experiment revealed that the selected aptmers possessed a high binding affinity and specificity for S. sonnei. In this study, we demonstrated the feasibility of an aptamer sensor platform to detect S. sonnei in a variety of foods and pave the way for its use in diagnosing shigellosis through multiple, portable designs."
Additional information: Cy5 for detection probe at the 5' end; 5′-biotin-modified
Reference (2017, Molecules ): Song, M. S., Sekhon, S. S., Shin, W. R., Kim, H. C., Min, J., Ahn, J. Y., & Kim, Y. H. (2017). Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor Platform. Molecules (Basel, Switzerland), 22(5), 825. https://doi.org/10.3390/molecules22050825
Aptamer: SS-4
Target: Shigella sonnei (S. sonnei)
Type of nucleic acid: ssDNA
Sequence: 5'ATACCAGCTTATTCAATTCCACACATACCAAAAACACAGCACACTTCATCAATTTCACGAGATTGCACTTACTATCT3'
Sequence length: 77
GC content: 0.3636363636363636
Affinity: Kd: 15.89 ± 1.77 nM
Pool type: 5′-ATACCAGCTTATTCAATT-N40-AGATAGTAAGTGCAATCT-3′
Pool random region: 40
Binding buffer/conditions: TBS buffer (10 mM Tris-HCl, 0.85% NaCl, pH 8.0)
Buffer type: Tris Buffers
pH: 8
Molecular weight of target: Not reported
Post-SELEX modifications: 5' amine for capture probe
Application as quoted in the referenced paper: Detection: " In this paper, a Whole-Bacteria SELEX (WB-SELEX) strategy was adopted to isolate specific aptamers against Shigella sonnei. Real-time PCR amplification and post-SELEX experiment revealed that the selected aptmers possessed a high binding affinity and specificity for S. sonnei. In this study, we demonstrated the feasibility of an aptamer sensor platform to detect S. sonnei in a variety of foods and pave the way for its use in diagnosing shigellosis through multiple, portable designs."
Additional information: 5′-biotin-modified
Reference (2017, Molecules ): Song, M. S., Sekhon, S. S., Shin, W. R., Kim, H. C., Min, J., Ahn, J. Y., & Kim, Y. H. (2017). Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor Platform. Molecules (Basel, Switzerland), 22(5), 825. https://doi.org/10.3390/molecules22050825
Aptamer: G-ap49
Target: Gremlin-1
Type of nucleic acid: ssDNA
Sequence: 5′GGACAAGAATCACCGCTCGCGTGCTCCAGTGGCTCTTCGGTACCAACCCAAAGTGACTCGTACAGGAGGCATACAG3′
Sequence length: 76
GC content: 0.5657894736842105
Affinity: Kd: 2.6 ± 0.4 nM
Pool type: 5′-GGACAAGAATCACCGCTC-N40-CGTACAGGAGGCATACAG-3′
Pool random region: 40
Binding buffer/conditions: SHMCK buffer (20 mM Hepes, 120 mM NaCl, 5 mM KCl, 1 mM MgCl2 and 1 mM CaCl2, pH 7.4) and elution buffer (7 M urea, 0.5 M NH4Ac, 1 mM EDTA, 0.2% SDS)
Divalent salt: MgCl/CaCl
Buffer type: Other Buffers
pH: 7.4
Molecular weight of target: 25 kDa
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Detection: " Gremlin-1, a highly conserved glycosylated and phosphorylated secretory protein, plays important roles in diverse biological processes including early embryonic development, fibrosis, tumorigenesis, and renal pathophysiology. Our study demonstrated that G-ap49 is capable of revealing the subcellular localization of Gremlin-1. These data indicate that G-ap49 can be used as an alternative to antibodies in detecting Gremlin-1."
Reference (2017, Molecules ): Li, Q., Huo, Y., Guo, Y., Zheng, X., Sun, W., & Hao, Z. (2017). Generation and Applications of a DNA Aptamer against Gremlin-1. Molecules (Basel, Switzerland), 22(5), 706. https://doi.org/10.3390/molecules22050706
Aptamer: Ama1 (A2-3)
Target: Alpha-amanitin (α-amanitin) from Amanita phalloides (A. phalloides)
Type of nucleic acid: ssDNA
Sequence: 5'CATGCTTCCCCAGGGAGATGGAGGTCTTTTTGGTTGTTGGTGGGGGAATCTTTTGGTATTGAGGAACATGCGTCGCAAAC3'
Sequence length: 80
GC content: 0.5
Affinity: Kd: 5.026 ± 0.69 uM
Pool type: 5'-CATGCTTCCCCAGGGAGATG-N40-GAGGAACATGCGTCGCAAAC-3'
Pool random region: 40
Binding buffer/conditions: AS buffer (137 mM NaCl, 12.3 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4,5 mM MgCl2, 0.01% (v/v) Tween 20; pH 7.5)
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.5
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Therapeutic: " The group of mushrooms highly dangerous for human health includes Amanita phalloides. This mushroom produces a toxic octapeptide called α-amanitin which is an inhibitor of nuclear RNA polymerase II. Since there is no effective antidote against amanitin poisoning, in this study we developed a DNA aptamer exhibiting specific binding to α-amanitin. Its ability of toxin removal from aqueous solution was confirmed by pull-down assay"
Reference (2017, Acta Biochim Pol ): Muszyńska, K., Ostrowska, D., Bartnicki, F., Kowalska, E., Bodaszewska-Lubaś, M., Hermanowicz, P., Faulstich, H., & Strzałka, W. (2017). Selection and analysis of a DNA aptamer binding α-amanitin from Amanita phalloides. Acta biochimica Polonica, 64(3), 401–406. https://doi.org/10.18388/abp.2017_1615
Aptamer: Ep1
Target: Epithelial cell adhesion molecule (EpCAM)
Type of nucleic acid: ssDNA
Sequence: 5'GGGATGGATCCAAGCTTACTGGCTCATTGGGTGGTGGCCTCTGGACGGGTTGTATGCGATGAGCGCAGGGAAGCTTCGATAGGAATTCGG3'
Sequence length: 90
GC content: 0.5666666666666667
Affinity: Kd: 118 nM
Pool type: 5'-GGGATGGATCCAAGCTTACTGG-45N-GGGAAGCTTCGATAGGAATTCGG-3'
Pool random region: 45
Binding buffer/conditions: PBS, 5 mM MgCl2
Divalent salt: MgCl
Buffer type: PBS/phosphate buffers
pH: 7.4
Molecular weight of target: 33–40 kDa
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic and Drug Delivery: " Epithelial cell adhesion molecule (EpCAM) is considered as a cancer stem cell (CSC) biomarker and one of the most promising targets for aptamer selection against CSCs. In this study, we have developed a ssDNA aptamer with high affinity and selectivity of targeting the EpCAM protein extracellular domain. The results of this study could suggest that Ep1 aptamer can bind specifically to the cellular EpCAM protein, making it an attractive ligand for targeted drug delivery and as an imaging agent for the identification of cancer cells."
Reference (2017, PLoS One ): Alshaer, W., Ababneh, N., Hatmal, M., Izmirli, H., Choukeife, M., Shraim, A., Sharar, N., Abu-Shiekah, A., Odeh, F., Al Bawab, A., Awidi, A., & Ismail, S. (2017). Selection and targeting of EpCAM protein by ssDNA aptamer. PloS one, 12(12), e0189558. https://doi.org/10.1371/journal.pone.0189558
Aptamer: Apt22
Target: Antigen 85 (Ag85A) (FbpA)
Type of nucleic acid: ssDNA
Sequence: 5'GCTGTGTGACTCCTGCAAGCGGGAAGAGGGTAAGGGGAGGGAGGGTAACGCGGAGAAGGCAAGCAGCTGTATCTTGTCTCC3'
Sequence length: 81
GC content: 0.5925925925925926
Affinity: Kd: 62.95 nM
Pool type: 5′-GCTGTGTGACTCCTGCAA-N43-GCAGCTGTATCTTGTCTCC-3′
Pool random region: 43
Binding buffer/conditions: PBS-T containing 1 mg.mL−1 bovine serum albumin (BSA)
Buffer type: PBS/phosphate buffers
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis. It is a potential marker for early diagnosis of tuberculosis (TB). The authors have identified specific aptamers for Ag85A (FbpA) via protein SELEX using magnetic beads. On the basis of its performance, the GO-based fluorescent aptasensor revealed a promising future for the detection of protein biomarkers of M. tuberculosis"
Reference (2017, Mikrochim Acta ): Ansari, N., Ghazvini, K., Ramezani, M., Shahdordizadeh, M., Yazdian-Robati, R., Abnous, K., & Taghdisi, S. M. (2017). Selection of DNA aptamers against Mycobacterium tuberculosis Ag85A, and its application in a graphene oxide-based fluorometric assay. Mikrochimica acta, 185(1), 21. https://doi.org/10.1007/s00604-017-2550-3
Aptamer: Apt8
Target: Antigen 85 (Ag85A) (FbpA)
Type of nucleic acid: ssDNA
Sequence: 5'GCTGTGTGACTCCTGCAATCAGGAAAGAACTTAGGGGTGGGAGGAGGGTATAAATACGGAATGCAGCTGTATCTTGTCTCC3'
Sequence length: 81
GC content: 0.4938271604938271
Affinity: Kd: 202 nM
Pool type: 5′-GCTGTGTGACTCCTGCAA-N43-GCAGCTGTATCTTGTCTCC-3′
Pool random region: 43
Binding buffer/conditions: PBS-T containing 1 mg.mL−1 bovine serum albumin (BSA)
Buffer type: PBS/phosphate buffers
Molecular weight of target: Not reported
Post-SELEX modifications: Not applicable
Application as quoted in the referenced paper: Diagnostic: " The Mycobacterium Ag85 complex is the major secretory protein of M. tuberculosis. It is a potential marker for early diagnosis of tuberculosis (TB). The authors have identified specific aptamers for Ag85A (FbpA) via protein SELEX using magnetic beads. On the basis of its performance, the GO-based fluorescent aptasensor revealed a promising future for the detection of protein biomarkers of M. tuberculosis"
Reference (2017, Mikrochim Acta ): Ansari, N., Ghazvini, K., Ramezani, M., Shahdordizadeh, M., Yazdian-Robati, R., Abnous, K., & Taghdisi, S. M. (2017). Selection of DNA aptamers against Mycobacterium tuberculosis Ag85A, and its application in a graphene oxide-based fluorometric assay. Mikrochimica acta, 185(1), 21. https://doi.org/10.1007/s00604-017-2550-3