# Proteomika

Robotska priprema uzoraka za MALDI masenu spektrometriju.
**Proteomika** je izučavanje proteina velikih razmera sa fokusom na njihovu strukturu i funkcije. Proteini su vitalni delovi živih organizama. Oni su glavne komponente fizioloških metaboličkih puteva u ćelijama. Termin *proteomika* je formiran 1997 po analogiji sa genomikom, studijom genoma. Reč *proteom* je mešavina reči prote**in* i *gen**om, koju je formirao Mark Vilkins 1994.

Proteom je celokupan set proteina koji jedan organizam proizvodi ili modifikuje. Njegov sadržaj varira s vremenom i u zavisnosti od raznih uslova, stresova, kojima su ćelija ili ogranizam izloženi. Proteomika je interdisciplinarni domen koji je znatnim delom formiran na bazi istraživanja i razvoja Projekta ljudskog genoma. Ona obuhvata naučna istraživanja i ispitivanja proteoma sa sveobuhvatnog stanovišta intracelularne proteinske kompozicije, strukture, i njegovih jedinstvenih parterna dejstva. Proteomioka je važna komponenta funkcionalne genomike.

Mada se termin *proteomika* generalno odnosi na eksperimentalnu analizu proteina velikih razmera, on se isto tako koristi za proteinsko prečišćavanje i masenu spektrometriju.

## Reference

## Literatura

* Belhajjame, K. et al. Proteome Data Integration: Characteristics and Challenges Arhivirano 2006-06-28 na Wayback Machine-u. Proceedings of the UK e-Science All Hands Meeting, ISBN 1-904425-53-4, September 2005, Nottingham, UK.
* Twyman RM (2004). *Principles Of Proteomics (Advanced Text Series)*. Oxford, UK: BIOS Scientific Publishers. ISBN 1-85996-273-4.  (covers almost all branches of proteomics)
* Naven T, Westermeier R (2002). *Proteomics in Practice: A Laboratory Manual of Proteome Analysis*. Weinheim: Wiley-VCH. ISBN 3-527-30354-5.  (focused on 2D-gels, good on detail)
* Liebler DC (2002). *Introduction to proteomics: tools for the new biology*. Totowa, NJ: Humana Press. ISBN 0-89603-992-7.  ISBN 0-585-41879-9 (electronic, on Netlibrary?), ISBN 0-89603-991-9 hbk
* Wilkins MR, Williams KL, Appel RD, Hochstrasser DF (1997). *Proteome Research: New Frontiers in Functional Genomics (Principles and Practice)*. Berlin: Springer. ISBN 3-540-62753-7.
* Arora PS, Yamagiwa H, Srivastava A, Bolander ME, Sarkar G (2005). „Comparative evaluation of two two-dimensional gel electrophoresis image analysis software applications using synovial fluids from patients with joint disease”. *J Orthop Sci* **10** (2): 160–6. DOI:10.1007/s00776-004-0878-0. PMID 15815863.
* Rediscovering Biology Online Textbook. Unit 2 Proteins and Proteomics. 1997–2006.
* Weaver RF (2005). *Molecular biology* (3rd izd.). New York: McGraw-Hill. str. 840–9. ISBN 0-07-284611-9.
* Reece J, Campbell N (2002). *Biology* (6th izd.). San Francisco: Benjamin Cummings. str. 392–3. ISBN 0-8053-6624-5.
* Hye A, S, Lynham, M, Thambisetty i dr.. (November 2006). „Proteome-based plasma biomarkers for Alzheimer's disease”. *Brain* **129** (Pt 11): 3042–50. DOI:10.1093/brain/awl279. PMID 17071923.
* Perroud B, J, Lee, N, Valkova i dr.. (2006). „Pathway analysis of kidney cancer using proteomics and metabolic profiling”. *Mol Cancer* **5**: 64. DOI:10.1186/1476-4598-5-64. PMC 1665458. PMID 17123452.
* Yohannes E, Chang J, Christ GJ, Davies KP, Chance MR (July 2008). „Proteomics analysis identifies molecular targets related to diabetes mellitus-associated bladder dysfunction”. *Mol. Cell Proteomics* **7** (7): 1270–85. DOI:10.1074/mcp.M700563-MCP200. PMC 2493381. PMID 18337374. .
* Macaulay IC, Carr P, Gusnanto A, Ouwehand WH, Fitzgerald D, Watkins NA (December 2005). „Platelet genomics and proteomics in human health and disease”. *J Clin Invest.* **115** (12): 3370–7. DOI:10.1172/JCI26885. PMC 1297260. PMID 16322782.
* Rogers MA, P, Clarke, J, Noble i dr.. (2003-10-15). „Proteomic profiling of urinary proteins in renal cancer by surface enhanced laser desorption ionization and neural-network analysis: identification of key issues affecting potential clinical utility”. *Cancer Res.* **63** (20): 6971–83. PMID 14583499.
* Vasan RS (May 2006). „Biomarkers of cardiovascular disease: molecular basis and practical considerations”. *Circulation* **113** (19): 2335–62. DOI:10.1161/CIRCULATIONAHA.104.482570. PMID 16702488.
* "Myocardial Infarction" Arhivirano 2006-12-06 na Wayback Machine-u. (Retrieved 29 November 2006)
* Introduction to Antibodies – Enzyme-Linked Immunosorbent Assay (ELISA). (Retrieved 29 November 2006)
* Decramer S, S, Wittke, H, Mischak i dr.. (April 2006). „Predicting the clinical outcome of congenital unilateral ureteropelvic junction obstruction in newborn by urinary proteome analysis”. *Nat Med.* **12** (4): 398–400. DOI:10.1038/nm1384. PMID 16550189.
* Mayer U (January 2008). „Protein Information Crawler (PIC): extensive spidering of multiple protein information resources for large protein sets”. *Proteomics* **8** (1): 42–4. DOI:10.1002/pmic.200700865. PMID 18095364.
* Jörg von Hagen, VCH-Wiley 2008 *Proteomics Sample Preparation. ISBN 978-3-527-31796-7*

## Povezano

* Bioinformatika
* Citomika
* Genomika
* Lipidomika

## Vanjske veze

* Proteomics na Projektu Open Directory