Intro to Light Microscopy 1: Microscopy Basics
Channel: Microscopy Australia
Date: 10/07/2020

In this module you will learn the basics of light microscopy including 
• Basic light microscope components – 04:15
• Brightfield imaging and related modalities – 08:20
• Basic sample preparation for brightfield microscopy – 16:33
• Key terms and considerations – 19:21

Learning Objectives Include:
• Brightfield LightPath: Kohler Illumination
• Phase Contrast
• Differential Interference Contrast
• Polarization Microscopy
• Darkfield
• Sample preparation: fixation, sectioning, labelling, mounting
• Resolution, Numerical Aperture, Refractive Index
• Digital Cameras

Presented by Dr. Courtney Wright from Sydney Microscopy and Microanalysis, a Microscopy Australia facility.


These videos provide guidance only. Local work health and safety protocols should always take precedence when you undertake any lab procedures in your own facility. Some of the videos show workflows for specific instruments and their content should not be taken as specific instructions for other types of instruments.