How to Sequence a Human Genome in 7 'Easy' Steps!
Channel: National Human Genome Research Institute
Date: 2023-03-02

Join NHGRI researchers at the NIH Intramural Sequencing Center (NISC) as they show you step-by-step how to extract human DNA, sequence it, and interpret the data -- with some fun DNA high jinks along the way. 

Step 1: Find a DNA sample.  
Step 2: Isolate the cells by centrifugation.  
Step 3: Extract the DNA from the sample. 
Step 4: Load the sample into the sequencer. 
Step 5: Wait for the data to be generated. 
Step 6: Use the data you have collected to build the genome. 
Step 7: Analyze the DNA sequence data for quality. 
Done! High five your team! 

#t2t 

Learn more about how scientists completed the first complete, gapless sequence of a human genome: https://www.genome.gov/T2T 

More about The NIH Intramural Sequencing Center (NISC) https://www.nisc.nih.gov/  

**Note: Methods have been oversimplified for communications purposes. Sequencing a full human genome is actually quite challenging!**

Credits:
Producer/Writer/Director: Jenny Montooth, M.A., NHGRI
Videographer/Animator/Editor: Ernesto Del Aguila III, NHGRI
Cameraperson: Darryl Leja, M.A., NHGRI
Audio: Alvaro Encinas, NHGRI
Executive Producer: Sarah Bates, M.A., M.S., NHGRI
Starring: Adam Phillippy, Ph.D., NHGRI
Shelise Brooks, NISC
Sergey Koren, Ph.D., NHGRI
Arang Rhie, Ph.D., NHGRI
Brandon Pickett, Ph.D., NHGRI