Fitting mechanisms to single ion channel records. The Plymouth lecture.-long version
Channel: David Colquhoun
Date: 2021-09-12

This lecture takes just over two hours, so take as many breaks as you need.  It's based on a lecture that I gave on the excellent practical workshop, Microelectrode Techniques. This course has been run at the Plymouth Marine Biology Lab every year since 1984. It is organised heroically by David Ogden.  In 2020 and 2021 the course has fallen victim to Covid-19, and this video was made for the 2021 course, which had to be online only.

There is also a 1 hour version of this talk. at https://youtu.be/JRW6gMlXKr8 which was designed to be sandwiched between two talks by Lucia Sivilotti. The first of  these was a general introduction to single ion channels. The second illustrated the use of maximum likelihood fitting to elucidate mechanisms of action of agonists.

Here are some useful references.

Colquhoun, D, Basic matrix algebra in 45 minutes https://www.youtube.com/watch?v=u_RE2yKyh0o

Colquhoun, D., & Hawkes, A. G. (1995a). The principles of the stochastic interpretation of ion channel mechanisms. In B. Sakmann & E. Neher (Eds.), Single Channel Recording (pp. 397-482). New York: Plenum Press. 

Colquhoun, D., & Hawkes, A. G. (1995b). A Q-matrix Cookbook. In B. Sakmann & E. Neher (Eds.), Single Channel Recording (pp. 589-633). New York: Plenum Press.

Colquhoun, D., & Hawkes, A. G. (1995). Desensitization of N-methyl-D-aspartate receptors: a problem of interpretation. Proceedings of the National Academy of Science U. S. A, 92, 10327-10329   Available at http://www.onemol.org.uk/?page_id=175#ch95