Chapter 42 Chip binding assay  - adhesion of malaria parasites under fluid flow
Channel: Malaria Methods & More
Date: 2022-05-08

This video is an accompaniment to Chapter 42 and details the steps needed to perform flow adhesion assays. Using commercial or self-fabricated slides/capillary tubes, it is possible to mimic the conditions found within blood vessels. These channels can be coated with endothelial cells, or with recombinant proteins and cell suspensions containing Plasmodium falciparum trophozoites can be assessed for they ability to bind. Antibodies targeting surface receptors such as ICAM-1 can be added to determine the effect upon adhesion. Alternatively, antibodies generated against PfEMP-1 proteins can be added to measure their ability to inhibit or reverse adhesion. 

The full written protocol is available online.