Blot (biology) | Wikipedia audio article
Channel: wikipedia tts
Date: 29/12/2018

This is an audio version of the Wikipedia Article:
https://en.wikipedia.org/wiki/Blot_(biology)


00:01:16 1 Southern blot
00:01:46 2 Western blot
00:02:16 3 Far-Western blot
00:02:48 4 Southwestern blot
00:03:21 5 Eastern blot
00:03:56 6 Far-Eastern blot
00:04:28 7 Northern blot
00:04:56 8 Reverse Northern blot
00:05:19 9 Dot blot
00:05:43 10 List of blots
00:06:15 11 See also



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SUMMARY
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A blot, in molecular biology and genetics, is a method of transferring proteins, DNA or RNA, onto a carrier (for example, a nitrocellulose, polyvinylidene fluoride (PVDF) or nylon membrane). In many instances, this is done after a gel electrophoresis, transferring the molecules from the gel onto the blotting membrane, and other times adding the samples directly onto the membrane. After the blotting, the transferred proteins, DNA or RNA are then visualized by colorant staining (for example, silver staining of proteins), autoradiographic visualization of radioactive labelled molecules (performed before the blot), or specific labelling of some proteins or nucleic acids. The latter is done with antibodies or hybridization probes that bind only to some molecules of the blot and have an enzyme joined to them. After proper washing, this enzymatic activity (and so, the molecules we search in the blot) is visualized by incubation  with proper reactive, rendering either a colored deposit on the blot or a chemiluminiscent reaction which is registered by photographic film.