Insertional inactivation using Lac Z gene (Blue white screening) | Biotechnology | Khan Academy
Channel: Khan Academy India - English
Date: 2021-10-14

In this video, let's explore how we can use an enzyme producing selectable marker to select for the right cells. We will take the example of Lac Z gene, which naturally digests Beta galactosidase, and see how using X-gal, we can screen for the recombinants. This method takes less steps than using multiple antibiotic markers, is quicker, and therefore more suited. 

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Created by Khadijatul Qubra