Protein engineering | Wikipedia audio article
Channel: wikipedia tts
Date: 05/07/2019

This is an audio version of the Wikipedia Article:
https://en.wikipedia.org/wiki/Protein_engineering


00:00:55 1 Approaches
00:01:04 1.1 Rational design
00:02:22 1.2 Multiple sequence alignment
00:03:46 1.2.1 Clustal omega
00:04:18 1.2.2 MAFFT
00:04:43 1.2.3 K-Align
00:04:59 1.2.4 Multiple sequence comparison by log expectation (MUSCLE)
00:05:19 1.2.5 T-Coffee
00:05:39 1.3 Coevolutionary analysis
00:06:55 1.4 Structural Prediction
00:07:28 1.4.1 iAb initio/i
00:08:32 1.4.2 Fragment based
00:09:14 1.4.3 Homology modeling
00:10:22 1.4.4 Protein threading
00:11:34 1.5 Directed evolution
00:15:57 1.6 Asexual methods
00:16:23 1.6.1 Random mutagenesis
00:16:53 1.6.2 Error prone PCR
00:18:48 1.6.3 Rolling circle error-prone PCR
00:20:26 1.6.4 Chemical mutagenesis
00:21:34 1.6.5 Targeting glycosylases to embedded arrays for mutagenesis (TaGTEAM)
00:22:08 1.6.6 Mutagenesis by random insertion and deletion
00:22:39 1.6.7 Transposon based random mutagenesis
00:23:23 1.6.8 Random mutagenesis methods altering the target DNA Length
00:23:56 1.6.9 Mutator strains
00:24:37 1.6.10 Focused mutagenesis
00:25:13 1.6.11 Site saturation mutagenesis
00:26:46 1.6.12 Sequence saturation mutagenesis (SeSaM)
00:27:33 1.6.13 Single primer reactions in parallel (SPRINP)
00:28:02 1.6.14 Mega primed and ligase free focused mutagenesis
00:28:32 1.6.15 Ω-PCR
00:28:53 1.6.16 PFunkel-ominchange-OSCARR
00:29:34 1.6.17 Trimer-dimer mutagenesis
00:29:48 1.6.18 Cassette mutagenesis
00:30:57 1.7 Sexual methods
00:31:28 1.7.1 iIn vitro/i homologous recombination
00:32:07 1.7.2 DNA shuffling
00:32:52 1.7.3 Random priming iIn vitro/i recombination (RPR)
00:33:54 1.7.4 Truncated metagenomic gene-specific PCR
00:34:32 1.7.5 Staggered extension process (StEP)
00:35:32 1.7.6 Random chimeragenesis on transient templates (RACHITT)
00:36:40 1.7.7 Synthetic shuffling
00:37:01 1.7.8 iIn vivo/i Homologous Recombination
00:37:31 1.7.9 Mutagenic organized recombination process by homologous iin vivo/i grouping (MORPHING)
00:37:56 1.7.10 Phage-assisted continuous evolution (PACE)
00:38:30 1.8 iIn vitro/i non-homologous recombination methods
00:38:50 1.9 Exon shuffling
00:40:07 1.9.1 Incremental truncation for the creation of hybrid enzymes (ITCHY)
00:41:43 1.9.2 SCRATCHY
00:42:31 1.9.3 Recombined extension on truncated templates (RETT)
00:43:27 1.9.4 Sequence homology-independent protein recombination (SHIPREC)
00:44:23 1.9.5 Sequence independent site directed chimeragenesis (SISDC)
00:45:18 1.9.6 Degenerate homo-duplex recombination (DHR)
00:46:00 1.9.7 Random multi-recombinant PCR (RM-PCR)
00:46:27 1.9.8 User friendly DNA recombination (USERec)
00:47:24 1.9.9 Golden Gate shuffling (GGS) recombination
00:48:16 1.9.10 Phosphoro thioate-based DNA recombination method (PRTec)
00:49:11 1.9.11 Integron
00:49:46 1.9.12 Y-Ligation based shuffling (YLBS)
00:51:07 2 Semi-rational design
00:53:49 3 Screening and selection techniques
00:55:20 3.1 Enzyme engineering
00:56:08 4 Examples of engineered proteins



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SUMMARY
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Protein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research taking place into the understanding of protein folding and recognition for protein design principles.  It is also a product and services market, with an estimated value of $168 billion by 2017.There are two general strategies for protein engineering: rational protein design and directed evolution.  These methods are not mutually exclusive; researchers will often apply both.  In the future, more detailed knowledge of protein structure and function, and advances in high-throughput screening, may greatly expand the abilities of protein engineering.  Eventually, even unnatural amino acids may be included, via newer methods, such as expanded genetic code, that allow encoding novel amino acids in genetic code.