Chemical ligation | Wikipedia audio article
Channel: wikipedia tts
Date: 2019-02-18

This is an audio version of the Wikipedia Article:
https://en.wikipedia.org/wiki/Chemical_ligation


00:00:46 1 Methods of chemical ligation
00:02:26 1.1 Expressed protein ligation
00:03:21 1.2 Staudinger ligation
00:03:54 1.3 Ser/Thr ligation



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SUMMARY
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Chemical ligation is a set of techniques used for creating long peptide or protein chains. It is the second step of a convergent approach. First, smaller peptides containing 30-50 amino acids are prepared by conventional chemical peptide synthesis. Then, they are completely deprotected. Chemical ligation is the technique of coupling these peptides by chemoselective reaction to give a unique reaction product, usually in aqueous solution. With several coupling steps, proteins of up to 200-300 amino acids can be produced.