Tomas Kirchhausen - Imaging subcellular dynamics from molecules to multicellular organisms
Channel: Institut des Hautes Études Scientifiques (IHÉS)
Date: 2018-03-26

Frontier optical-imaging modalities exemplified by the lattice light-
sheet microscope invented by Eric Betzig sets new visualization standards for analyzing and understanding sub-cellular processes in the complex and dynamic three-dimensional environment of living-cells in isolation and within tissues of an organism. By using ultra-thin sheets of light to rapidly illuminate biological samples with extremely low photon doses, 3D experiments previously limited to seconds or minutes by photo-bleaching or by photo-
toxicity, can now be done at diffraction limited resolution and high-temporal precision with unprecedented duration of minutes or hours.
We believe this ability to image with minimal perturbations  is  ideally  suited  to  support hypothesis-generating  research  geared  towards  new discoveries.
The talk will illustrate how we use lattice light-sheet microscopy to ‘see’ in three dimensions the intracellular  delivery  of  RNAi  and  antisense  oligonucleotides  in  cells  maintained  in  tissue  culture
conditions and also will describe our most recent efforts using
lattice light sheet microscopy with adaptive  optics  to  link  processes  that  mediate  and  regulate  the  movement  of  vesicular  carriers throughout cells and the biogenesis of organelles in both, isolated cells maintained in tissue culture 
conditions and cells within tissues of a living zebrafish embryo