Annealing (biology) | Wikipedia audio article
Channel: wikipedia tts
Date: 28/03/2019

This is an audio version of the Wikipedia Article:
https://en.wikipedia.org/wiki/Nucleic_acid_thermodynamics


00:00:48 1 Concepts
00:00:57 1.1 Hybridization
00:02:46 1.2 Denaturation
00:05:55 1.3 Annealing
00:06:58 1.4 Stacking
00:07:06 2 Thermodynamics of the two-state model
00:12:38 3 Estimating thermodynamic properties from nucleic acid sequence
00:13:26 3.1 Nearest-neighbor method
00:21:59 4 See also



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SUMMARY
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Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA). The melting temperature (Tm) is defined as the temperature at which half of the DNA strands are in the random coil or single-stranded (ssDNA) state. Tm depends on the length of the DNA molecule and its specific nucleotide sequence. DNA, when in a state where its two strands are dissociated (i.e., the dsDNA molecule exists as two independent strands), is referred to as having been denatured by the high temperature.