Abi Perrin | CyanoTag: High-throughput protein tagging in a model photosynthetic bacterium
Channel: CyanoWorld
Date: 2023-11-05

Abi Perrin - University of York, UK

Homepage: https://pure.york.ac.uk/portal/en/persons/abi-perrin
Twitter: https://twitter.com/PerrinAbi

CyanoTag: High-throughput protein tagging in a model photosynthetic bacterium

Photosynthetic bacteria are fundamentally important to aquatic ecosystems and global carbon cycling and have exciting potential for use in sustainable bioindustries. However, much of their basic biology is not yet understood and their genomes still contain large numbers of uncharacterised protein-coding genes. To accelerate our understanding of these essential microorganisms we have developed a high-throughput pipeline for the scarless integration of tags that facilitate protein localisation and interactome profiling in the model cyanobacterium Synechococcus elongatus PCC 7942. We have used this CyanoTag platform to fluorescently label over 320 S. elongatus proteins – more than 10% of the proteome. Localisation of these proteins via super-resolution imaging has provided novel biological insights into a diverse range of processes including the regulation of photosynthesis and cell division. We are now expanding the CyanoTag library to the whole S. elongatus proteome, building an interactome, and collaborating to increase the benefit of the CyanoTag resource for the wider research community.

This talk is part of the CyanoWorld online seminar series, hosted by Nicolas Schmelling and Ilka Axmann, and was held on Zoom on August 11th, 2022.

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