Protein expression (biotechnology) | Wikipedia audio article
Channel: wikipedia tts
Date: 03/05/2019

This is an audio version of the Wikipedia Article:
https://en.wikipedia.org/wiki/Protein_production


00:01:16 1 Protein production systems
00:01:51 1.1 Cell-based systems
00:04:59 1.1.1 Bacterial systems
00:05:08 1.1.1.1 iEscherichia coli/i
00:06:29 1.1.1.2 iCorynebacterium/i
00:07:26 1.1.1.3 iPseudomonas fluorescens/i
00:08:04 1.1.2 Eukaryotic systems
00:08:13 1.1.2.1 Yeasts
00:08:36 1.1.2.2 Filamentous fungi
00:09:08 1.1.2.3 iBaculovirus/i-infected cells
00:09:46 1.1.2.4 Non-lytic insect cell expression
00:11:00 1.1.2.5 iExcavata/i
00:11:27 1.1.2.6 Mammalian systems
00:12:07 1.2 Cell-free systems
00:12:42 2 See also



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SUMMARY
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Protein production is the biotechnological process of generating a specific protein. It is typically achieved by the manipulation of gene expression in an organism such that it expresses large amounts of a recombinant gene. This includes the transcription of the recombinant DNA to messenger RNA (mRNA), the translation of mRNA into polypeptide chains, which are ultimately folded into functional proteins and may be targeted to specific subcellular or extracellular locations.Protein production systems (in lab jargon also referred to as 'expression systems') are used in the life sciences, biotechnology, and medicine. Molecular biology research uses numerous proteins and enzymes, many of which are from expression systems; particularly DNA polymerase for PCR, reverse transcriptase for RNA analysis, restriction endonucleases for cloning, and to make proteins that are screened in drug discovery as biological targets or as potential drugs themselves. There are also significant applications for expression systems in industrial fermentation, notably the production of biopharmaceuticals such as human insulin to treat diabetes, and to manufacture enzymes.