ISMARA: Integrated System for Motif Activity Response Analysis.
Channel: ISMARA: inferring gene regulatory interactions
Date: 2022-11-07

This video provides an overview of Integrated System for Motif Activity Response Analysis (https://ismara.unibas.ch). We briefly introduce Motif Activity Response Analysis (MARA) model, show the web service interface and go over results of the ISMARA analysis.

The example results of the ISMARA analysis used in this video are available here:
https://www.ismara.unibas.ch/ISMARA/scratch/GSE58827/ismara_report/

We also recommend a deep introduction to the ISMARA analysis tool: https://www.youtube.com/watch?v=Nj9r3t3Js5M

and detailed overview of the ISMARA interface:
https://www.youtube.com/watch?v=u5o-C7F05QI

Chapters
00:00 Outline
00:23 Example dataset
00:50 MARA linear model
02:23 Individual promoter expression fit
03:23 Main results page
03:41 Motif page (E2F2_E2F5)
04:12 Activity profile
04:50 Activity-expression correlation
05:36 Z-values Barnhart
05:50 Regulator targets table and target significance
07:01 Promoter fit page
07:33 Target analysis with StringDB 
08:03 Pathway enrichment
08:17 Regulator interaction network
08:48 Navigation and downloads