Allele-specific oligonucleotide
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Date: 2022-01-19

Allele-specific oligonucleotide, by Wikipedia https://en.wikipedia.org/wiki?curid=10099472 / CC BY SA 3.0

#DNA_profiling_techniques
#Molecular_biology
#Laboratory_techniques
#Molecular_biology_techniques
An allele-specific oligonucleotide （ASO） is a short piece of synthetic DNA complementary to the sequence of a variable target DNA. It acts as a probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler Dot blot assay.
It is a common tool used in genetic testing, forensics, and Molecular Biology research.
An ASO is typically an oligonucleotide of 15–21 nucleotide bases in length.
It is designed （and used） in a way that makes it specific for only one version, or allele, of the DNA being tested.
The length of the ASO, which strand it is chosen from, and the conditions by which it is bound to （and washed from） the target DNA all play a role in its specificity.
These probes can usually be designed to detect a difference of as little as 1 base in the target's genetic sequence, a basic ability in the assay of single-nucleotide polymorphisms （SNPs）, important in genotype analysis and the Human Genome Project.
To be detected after it has bound to its target, the ASO must be labeled with a radioactive, enzymatic, or fluorescent tag.
The Illumina Methylation Assay technology takes advantage of ASO to detect one base pair difference （cytosine versus thymine） to measure methylation at a specific CpG site.
Binding of the "S" ASO probe to "S" DNA （top） or "A" DNA （bottom）.
The human disease sickle cell anemia is caused by a genetic mutation in the codon for the sixth amino acid of the blood protein beta-hemoglobin.
The normal DNA sequence G-A-G codes for the amino acid glutamate, while the mutation changes the middle adenine to a thymine, leading to the sequence G-T-G （G-U-G in the mRNA）.
This altered sequence substitutes a valine into the final protein, distorting its structure.
To test for the presence of the mutation in a DNA sample, an AS...