Fluxomics by NMR (or how bacteria reproduce themselves) - Dr. Guy LIPPENS, TBI, Toulouse.
Channel: IPBS-Toulouse
Date: 04/03/2021

Measuring the carbon isotopic content of different metabolites is a powerful tool to quantify fluxes in living systems. Spanning the different forms of life, the approach has found applications in the study of bacteria, yeast, plants, or humans. In the latter case, the altered metabolism of cancer cells has attracted significant interest.

Here, we will present our efforts to develop NMR methodology to study the central metabolism of bacteria. Using both the 15N and 13C nuclei as an editing tool for characterization of the different metabolites,  we will use amino acid biosynthesis as an example to illustrate the power of the method. We finally will detail an improved NMR workflow aimed at assigning the high-resolution sub-spectrum of the phospho-metabolites in a complex mixture.