Cell Culture Basics 04: Adding and removing sterile liquid from a container
Channel: nanohubtechtalks
Date: 2015-09-23

This video is part of the introductory training for Cell Culture Basics. This learning unit is part of the training required for access to the Three Dimensional Cell Culture Core (3D3C) facility at Purdue University.  The accompanying PowerPoint file and documents for this training can be found on nanoHUB.org at:
http://nanohub.org/resources/22645

The videos provide some detailed demonstration of processes that help keep cell culture safe for all users as well as for the cultured cells during freezing, thawing and passaging the cells.  Specific information is given regarding aseptic techniques while manipulating cells and culture related items, tips on labeling culture vessels and other items, methods for adding or removing medium from cell culture vessels and seeding cells in cell culture vessels. These training videos also highlight simple rules to dispose of biohazardous sharps and clean the system used to aspirate the medium from cell cultures.