GENE EDITING EXPLAINED! - A comprehensive guide to the principles, methods and technologies!
Channel: Genomics Guru
Date: 2020-02-19

An “In Depth” lecture on gene editing brought to you by Dr Adam West, College of Medical Veterinary and Life Sciences at the University of Glasgow, Scotland.
https://www.gla.ac.uk/people/adamwest

Time stamps:
3:41          Introduction
9:04          DNA repair pathways
17:20        Possible genome editing outcomes using sequence-specific nucleases
23:32        Zinc finger nucleases (ZFNs)
34:38        Transcription activator-like effector nucleases (TALENs) 
44:09        CRISPR adaptive immune systems
50:01        Using CRISPR/Cas9 for genome editing
1:10:54     CRISPR specificity
1:35:52     Precision editing
1:40:00     Homology directed repair
1:44:19     Enzymatic base editing
1:47:20     Prime editing

The presentation is primarily aimed at university students, researchers, clinicians and journalists interested in fields related to molecular biology and genetics. It is for education purposes only.

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More accessible presentations for a wide audience can be found in the “Explained Simply” section of this channel in the near future.

Artwork is © Adam West with the exception of images and data taken from publications referenced in these slides. References to reviews and journal articles are denoted by circled numbers at the bottom right of the slides. 

Links to these numbered articles are listed below. We recommend these as your best way to begin further reading on this subject.

Publication List

1. Wah et al. Nature. 1997 Jul 3;388(6637):97-100. 
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2. Wah et al. Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10564-9. 
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC27934/

3. Ceccaldi R et al. Trends Cell Biol. 2016 Jan;26(1):52-64. 
https://doi.org/10.1016/j.tcb.2015.07.009

5. Wolfe SA et al. J Mol Biol. 1999 Feb 5;285(5):1917-34. 
https://doi.org/10.1006/jmbi.1998.2421

6. Wolfe SA et al. Annu Rev Biophys Biomol Struct. 2000;29:183-212. 
https://doi.org/10.1146/annurev.biophys.29.1.183

7. Klug A. Q Rev Biophys. 2010 Feb;43(1):1-21. 
https://doi.org/10.1017/S0033583510000089

8. Urnov FD et al. Nat Rev Genet. 2010 Sep;11(9):636-46. 
https://doi.org/10.1038/nrg2842

9. Carroll D. Genetics. 2011 Aug;188(4):773-82. 
https://doi.org/10.1534/genetics.111.131433

10. Kim H and Kim JS. Nat Rev Genet. 2014 May;15(5):321-34. 
https://doi.org/10.1038/nrg3686

12. Boch J and Bonas U. Annu Rev Phytopathol. 2010;48:419-36. 
https://doi.org/10.1146/annurev-phyto-080508-081936

13. Bogdanove AJ et al. Curr Opin Plant Biol. 2010 Aug;13(4):394-401. 
https://doi.org/10.1016/j.pbi.2010.04.010

14. Mak AN et al. Science. 2012 Feb 10;335(6069):716-9. 
https://doi.org/10.1126/science.1216211

15. Joung JK and Sander JD. Nat Rev Mol Cell Biol. 2013 Jan;14(1):49-55. 
https://doi.org/10.1038/nrm3486

16. Mussolino C and Cathomen T. Curr Opin Biotechnol. 2012 Oct;23(5):644-50. 
https://doi.org/10.1016/j.copbio.2012.01.013

17. Mali P et al. Nat Methods. 2013 Oct;10(10):957-63. 
https://doi.org/10.1038/nmeth.2649

19. Garside EL and MacMillan AM. Nat Biotechnol. 2014 Apr;32(4):338-40. 
https://doi.org/10.1038/nbt.2872

20. Nishimasu H et al. Cell. 2014 Feb 27;156(5):935-49. 
https://doi.org/10.1016/j.cell.2014.02.001

21. Hsu PD et al. Cell. 2014 Jun 5;157(6):1262-78. 
https://doi.org/10.1016/j.cell.2014.05.010

22. Hendel A et al. Trends Biotechnol. 2015 Feb;33(2):132-40. 
https://doi.org/10.1016/j.tibtech.2014.12.001

23. Liang X et al. J Biotechnol. 2015 Aug 20;208:44-53. 
https://doi.org/10.1016/j.jbiotec.2015.04.024

24. Brinkman EK et al. Nucleic Acids Res. 2014 Dec 16;42(22):e168. 
https://doi.org/10.1093/nar/gku936

25. Koo T et al. Mol Cells. 2015 Jun;38(6):475-81. 
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30. Howden SE et al. Stem Cell Reports. 2016 Sep 13;7(3):508-517. 
https://doi.org/10.1016/j.stemcr.2016.07.001

31. Fu Y et al. Nat Biotechnol. 2014 Mar;32(3):279-284. 
https://doi.org/10.1038/nbt.2808

32. Slaymaker IM et al. Science. 2016 Jan 1;351(6268):84-8. 
https://doi.org/10.1126/science.aad5227

33. Kleinstiver BP et al. Nature. 2016 Jan 28;529(7587):490-5. 
https://doi.org/10.1038/nature16526

34. Komor AC et al. Cell. 2017 Jan 12;168(1-2):20-36. 
https://doi.org/10.1016/j.cell.2016.10.044

37. Hess GT et al. Mol Cell. 2017 Oct 5;68(1):26-43. 
https://doi.org/10.1016/j.molcel.2017.09.029

38. Gaudelli NM et al. Nature. 2017 Nov 23;551(7681):464-471. 
https://doi.org/10.1038/nature24644

39. Anzalone AV et al. Nature. 2019 Dec;576(7785):149-157. 
https://doi.org/10.1038/s41586-019-1711-4

Music credits
“Go, Icarus! Go!” by The Whole Other
“New Year” by Bad Snacks
(YouTube Audio Library)