金魚の発生学実験＃02: 人工授精: Artificial Fertilization: Ver 2.0: 20220509Ver
Channel: Laboratory of Aquatic Zoology
Date: 10/05/2022

Contents (目次)
0:00　Introduction (イントロ)
0:27　Day 1 (一日目：屋外水槽)
1:41　Finding matured females (卵を産みそうなメスについて)
2:09　Hormone injection (採卵誘発剤)
3:47　Day 2 (二日目)
3:55　Sperm retrieval (精子をとる)
4:50　Semen extender (人口精漿)
5:01　Sperm activity check (精子活性のチェック)
6:03　Dishes are coated with green tea (お茶でプラスチック皿の表面コート)
7:02　Eggs（卵）
7:32　Fertilization　（受精）
7:55　Egg maturation　（卵の成熟）
8:27　Bleaching (漂白）
9:10　Multiple males and females （複数の雄雌の組みわせで人工授精）
9:19　Incubation （インキュベーション）
9:35　Next video （次回の予告）
9:49　Ending （エンディング）

References:
Tsai, H.-Y., Chang, M., Liu, S.-C., Abe, G. and Ota, K.G. (2013), Embryonic development of goldfish (Carassius auratus): A model for the study of evolutionary change in developmental mechanisms by artificial selection. Dev. Dyn., 242: 1262-1283. Tsai, H.-Y., Chang, M., Liu, S.-C., Abe, G. and Ota, K.G. (2013), Embryonic development of goldfish (Carassius auratus): A model for the study of evolutionary change in developmental mechanisms by artificial selection. Dev. Dyn., 242: 1262-1283. https://doi.org/10.1002/dvdy.24022

Li, I.-J., Chang, C.-J., Liu, S.-C., Abe, G. and Ota, K.G. (2015), Postembryonic staging of wild-type goldfish, with brief reference to skeletal systems. Dev. Dyn., 244: 1485-1518. https://doi.org/10.1002/dvdy.24340

Li, I.-J., Lee, S.-H., Abe, G. and Ota, K.G. (2019), Embryonic and postembryonic development of the ornamental twin-tail goldfish. Dev Dyn, 248: 251-283. https://doi.org/10.1002/dvdy.15

#人工授精 #金魚 人工授精 やり方 簡単　#goldfish developmental biology #goldfish research #developmental biology #太田欽也 #太田 欽也