TALENs - TALE NUCLEASES - GENE EDITING EXPLAINED!
Channel: Genomics Guru
Date: 2020-02-18

This series of short presentations on gene editing is brought to you by Dr Adam West, College of Medical Veterinary and Life Sciences at the University of Glasgow, Scotland.
https://www.gla.ac.uk/people/adamwest

This presentation describes the architecture of TALE nucleases, which are engineered proteins used for genome editing. 

This is part of a series (https://www.youtube.com/playlist?list=PLi807-a75VPrFbunxfUGEQB_uMoYbqRWe) that also covers

Zinc finger nucleases (ZFNs) 
CRISPR adaptive immune systems
Using CRISPR/Cas9 for genome editing
CRISPR specificity
Precision editing
Homology directed repair
Prime editing

This presentation is primarily aimed at university students, researchers, clinicians and journalists interested in fields related to molecular biology and genetics. It is for education purposes only.

Please leave a comment to let us know whether this was helpful to you and what you think we should cover next. We are a new channel so please give us a like and share our video on your social media if you think others should see it. We have a lot of content coming up, so please subscribe!

More accessible presentations for a wide audience can be found in the “Explained Simply” section of this channel in the near future.

Artwork is © Adam West with the exception of images and data taken from publications referenced in these slides. References to reviews and journal articles are denoted by circled numbers at the bottom right of the slides.

Links to these numbered articles are listed below. We recommend these as your best way to begin further reading on this subject.

Publication List
10. Kim H, Kim JS. 
A guide to genome engineering with programmable nucleases. 
Nat Rev Genet. 2014 May;15(5):321-34. 
https://doi.org/10.1038/nrg3686

12. Boch J, Bonas U. Xanthomonas 
AvrBs3 family-type III effectors: discovery and function. 
Annu Rev Phytopathol. 2010;48:419-36. 
https://doi.org/10.1146/annurev-phyto-080508-081936

13. Bogdanove AJ, Schornack S, Lahaye T. 
TAL effectors: finding plant genes for disease and defense. 
Curr Opin Plant Biol. 2010 Aug;13(4):394-401. 
https://doi.org/10.1016/j.pbi.2010.04.010

14. Mak AN, Bradley P, Cernadas RA, Bogdanove AJ, Stoddard BL. 
The crystal structure of TAL effector PthXo1 bound to its DNA target. 
Science. 2012 Feb 10;335(6069):716-9. 
https://doi.org/10.1126/science.1216211

15. Joung JK, Sander JD. 
TALENs: a widely applicable technology for targeted genome editing. 
Nat Rev Mol Cell Biol. 2013 Jan;14(1):49-55. 
https://doi.org/10.1038/nrm3486

16. Mussolino C, Cathomen T. 
TALE nucleases: tailored genome engineering made easy. 
Curr Opin Biotechnol. 2012 Oct;23(5):644-50. 
https://doi.org/10.1016/j.copbio.2012.01.013

Music credits
“Go, Icarus! Go!” by The Whole Other
“New Year” by Bad Snacks
(YouTube Audio Library)