Single-cell enhancer RNA analysis in mouse embryonic stem cells @ NIG International symposium 2017
Channel: Togotv
Date: 04/07/2017

http://togotv.dbcls.jp/20170711.html

NIG International symposium 2017, Commemorating the 30th Anniversary of DDBJ was held in Mishima Citizens Cultual Hall in shizuoka, Japan. On the third day of the symposium (29 May), oral sessions were held. 
In this talk, Haruka Ozaki makes a presentation entitled "Single-cell enhancer RNA analysis in mouse embryonic stem cells". (18:27) 

1. Gene expression variability in single cells～(00:20) 
2. Do enhancers drive cell-to-cell gene expression variability?～(00:54) 
3. Difficulty in detecting eRNAs in single cells～(02:19) 
4. RamDA-seq: a single-cell full-length total RNA-seq method～(03:15) 
5. Benchmark 1: Diluted total RNA～(04:26) 
6. Two positive control sets of eRNA in mouse ES cells～(04:49) 
7. Benchmark 2: Living cells～(08:03) 
8. Cell-type specific eRNAs～(08:24) 
9. DNA-binding motifs of biologically-relevant transcription factors～(09:58) 
10. We sorted mouse ES cells at the G1 phase～(10:54) 
11.Single-cell variability in eRNA expression is NOT technical noise～(11:12) 
12. Highly variable eRNAs in mESC～(12:08) 
13. Example of highly variable eRNAs in mESC～(12:42) 
14. Weak correlation b/w mRNA and highly variable eRNA～(13:14) 
15. Conclusion～(14:35) 
16. Future work～(15:06) 
17. Implication to database～(17:00) 
18. Acknowledgements～(18:06) #togotv#DBCLS#bioinformatics #togotv#DBCLS#bioinformatics