TRANSFORMATION/GRIFFITH’S EXPERIMENT-1928/Griffith Effect /Avery MacLeod McCarthy Experiment
Channel: Harshvardhan Joshi
Date: 2021-05-26

TRANSFORMATION
GRIFFITH’S EXPERIMENT-1928
Griffith Effect

DNA, the Genetic Material

Streptococcus pneumoniae bacteria
There are two strains found
• RII-Non virulent and non-capsulated – its 
rough in appearance 
• SIII- Virulent and Capsulated 
(polysaccharide capsule)- its smooth in 
appearance. 

#molecularbiology


Avery–MacLeod–McCarty Experiment 1944

they did in vitro experiment. 

They take heat 🔥 killed S 3rd bacteria solution. they extract lipid and sugar from this solution. 
after this they  take this solution into three test tube. 
after this they mix Proteinase in first test tube, RNAase  in second test tube and DNAase  in third test tube. 

after the mixing these chemicals first test tube's protein was dissolved and second test tube's RNA was dissolved and third test tube's DNA was dissolved or destroyed. 


after some time they observed that in a first and  test tube S third bacteria was developed and in 3rd test tube there is not a strain of S third bacteria. 

it means that DNA is responsible for transformation. 

#microbialgenetics
#harshvardhanjoshi

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