Fluorescence Filters: Imaging Multiple Fluorophores & Dealing with Bleed-through
Channel: Microcourses
Date: 31/03/2020

*This video walks you through best practices for imaging more than one fluorophore, including critical controls experiments, why bleed-through can occur when imaging multiple fluorophores and various strategies for reducing bleed-through. Enjoy!

* Visit FPbase.org/microscopes to generate a custom spectraviewer for your microscope.
* Visit Image.sc for expert help with calculating bleedthrough coefficient and image arithmetic.
* Visit Forum.microlist.org for expert help on the microscopy and sample preparation methods discussed in this Microcourse.
* Jost & Waters, 2019: https://doi.org/10.1083/jcb.201812109
* French subtitles were provided by BioImaging North America.