Microgenomics 2021 webinar EV & Circulating Biomarkers: AHendrix
Channel: The International Symposium on Microgenomics 2023
Date: 2022-01-03

An Hendrix (Ghent University, Belgium) will present on "EV in liquid biopsies: from technological considerations towards biomarker discovery"

Separating extracellular vesicles (EVs) from blood plasma is challenging and complicates their biological understanding and biomarker development (PMID: 33568799). We fractionate blood plasma by combining size-exclusion chromatography (SEC) and OptiPrep density gradient centrifugation to study clinical context-dependent and time-dependent variations in the biomolecular landscape of systemically circulating EVs (PMID: 31776460; PMID: 34429857) . Using blood plasma samples from cancer patients and reference materials to ensure standardized EV measurements (PMID: 31337761; PMID: 33452501), we demonstrate the technical repeatability of blood plasma fractionation. Using serial blood plasma samples from cancer patients we show that EV carry a clinical context-dependent and/or time-dependent protein and small RNA composition, including miRNA and tRNA. In addition, differential analysis of blood plasma fractions provides 1) a catalogue of putative EV corona proteins, 2) a list of proteins not associated with systemically circulating EV, and 3) reveals the presence of systemically circulating bacterials EVs (PMID: 30518529). In conclusion, the implementation of blood plasma fractionation allows to advance the biological understanding and biomarker development of systemically circulating EV.