CH450 Chapter 7.3 Restriction Endonucleases
Channel: Patricia Flatt
Date: 2020-11-01

In this section, we use Type II Restriction Endonucleases (specifically EcoRI and BglII) to study enzyme strategies used for catalysis. Restriction enzymes (hydrolases) from this class, typically use strain distortion, catalysis by approximation, and cofactor catalysis in their general reaction mechanisms.