Dr. Guido Pintacuda - Fast magic-angle spinning NMR of membrane proteins
Channel: IPBS-Toulouse
Date: 2021-03-04

Online Seminar 

Building on a decade of continuous advances of the community, the recent development of very fast (60 kHz and above) magic-angle spinning (MAS) probes has revolutionised the field of solid-state NMR. Today, rapid “fingerprinting” of proteins by 1H detection is possible with a ten-fold reduction of the required sample amounts with respect to conventional approaches, not only in deuterated molecules but also in fully-protonated substrates. Extensive and robust resonance assignments can be derived rapidly for small-to-medium sized proteins (up to ca. 300 residues), opening the way to the determination of inter-nuclear proximities, relative orientations of secondary structural elements, protein-cofactor interactions, local and global dynamics.

Fast MAS and 1H detection techniques have nowadays been shown to be applicable to membrane-bound systems. This talk reviews the strategies underlying this recent leap forward in sensitivity and resolution, describing its potential for the detailed characterization of membrane proteins in lipid bilayers.

Dr Guido Pintacuda, Centre de RMN à Très Hauts Champs, CNRS/Université Claude Bernard Lyon 1/Ecole Normale Supérieure de Lyon, Villeurbanne, France