How to Do an Acid-fast Stain (Ziehl-Neelsen)
Channel: Tami Guy, MS, CPPS
Date: 2012-09-24

Step-by-step video and audio instructions on how to do an acid-fast stain (a.k.a. Ziehl Neelsen stain) of a slide that includes three bacterial smears; an acid-fast positive control (known acid-fast bacteria that has a waxy cell wall), a nonacid-fast negative control (known nonacid-fast bacteria that does not have a waxy cell wall) and an unknown.

For a video on how to prepare a bacterial smear for acid-fast staining, see: http://youtu.be/KRWxRp0oQeQ

Video by Tami (Guy) Port, Chief Executive Nerd at ScienceProfOnline.com. For the lab materials that go with this movie, see the Virtual Microbiology Classroom Identification of Unknown Bacteria Laboratory Main Page at ScienceProfOnline.com.