Primer Design for RNA/DNA amplification (Bioinformatics S9E2)
Channel: Danny Arends
Date: 2021-12-28

Advanced primer design, and a live example using Ensembl and primer3.  Live stream recording of the ninth lecture of the course "Bioinformatics for plant and animal science", during this lecture I give you an overview into Computer Aided Primer Design for polymerase chain reaction (PCR).

Lectures @ https://dannyarends.nl/bioinformatik/
Programming Assignments and Answers @ https://dannyarends.nl/bioinfo/
Playlist: https://www.youtube.com/playlist?list=PLhR2Go-lh6X5A5WbiO3SPHuoWbwpNznUl

Chapters:
00:00 Welcome back and quick summary
00:50 Advanced primer design
01:48 Multiplex Polymerase Chain Reaction (PCR) for multiple PCR targets
03:38 Universal primers to amplify gene families
08:50 Semi-universal primers to amplify targeted (e.g. deleterious) gene families
10:12 Guessmer probes based on back translated protein amino acid sequences
15:53 Advanced primer design summary
18:11 Example of using the Ensembl database and Primer3

This is a live-stream recording of the MSc and PhD lecture series: Bioinformatics for plant and animal sciences, given digitally during the Covid19 pandemic at the Humboldt University in Berlin organized and lectured by Dr Danny Arends.

Thanks for taking an interest in my channel 😄If you've made it this far down, support me by giving a like or subscribing. Join me during my live streams Thursday afternoons on Twitch @ https://www.twitch.tv/dannyarends

This work is licensed under version 3.0 of the Creative Commons CC-BY license. For more information see http://creativecommons.org/licenses/by/3.0/legalcode

#bioinformatics #ComputerAidedPrimerDesign #polymerasechainreaction #computationalbiology  #lectureseries #programmingforstudents #programmingforbeginners  #molecularbiology  #englishstream #msc #phd #livestream #computers #computerskills #live #assignments #statisticsfordatascience #academia #academicyoutube #primerdesign #pcr #amplification #polymerasechainreaction #polymerase #templateDNA  #guessmer #multiplex #primer3