Metabolites and Mass Spectrometry (Bioinformatics S6E2)
Channel: Danny Arends
Date: 2021-12-05

Learn how metabolomics uses Mass Spectrometry to determine metabolites in samples, and how fragment identification is performed using the METLIN database. We'll go into different ionization techniques used in metabolomics, and I do a viewer reward drawing of my Betta Mahachaiensis.

Lectures @ https://dannyarends.nl/bioinformatik/
Programming Assignments and Answers @ https://dannyarends.nl/bioinfo/
Playlist: https://www.youtube.com/playlist?list=PLhR2Go-lh6X5A5WbiO3SPHuoWbwpNznUl

Chapters:
00:00 Welcome
00:10 Primary metabolites
00:55 Secondary metabolites
01:31 Identification of #metabolites by #Mass #Spectrometry (MS)
03:27 Compound separation by chromatography
04:43 Compound separation by capillary electrophoresis
05:34 Hard ionization leads to compound fragmentation
09:41 Viewer Reward Drawing 2: Betta Mahachaiensis
24:17 Different soft ionization techniques
25:52 Ionization by fast atom bombardment
27:23 Chemical ionization of compounds
28:38 Creating big fragments using electrospray ionization 
30:42 Ionization by MALDI (matrix-assisted laser desorption/ionization)
32:20 Laws of Physics underlying mass separation
33:23 M/Z Separation by sector instrument / Time-Of-Flight (TOF)
36:04 M/Z Detection generates a mass spectra
37:36 The METLIN database to identify compounds in a mass spectrum
42:55 The four principles of metabolic pathways
50:23 Metabolic organelles in a eukaryotic cell

This is a live-stream recording of the MSc and PhD lecture series: Bioinformatics for plant and animal sciences, given digitally during the Covid19 pandemic at the Humboldt University in Berlin organized and lectured by Dr Danny Arends.

Thanks for taking an interest in my channel 😄If you've made it this far down, support me by giving a like or subscribing. Join me during my live streams Thursday afternoons on Twitch @ https://www.twitch.tv/dannyarends

This work is licensed under version 3.0 of the Creative Commons CC-BY license. 
For more information see http://creativecommons.org/licenses/by/3.0/legalcode

#bioinformatics #computationalbiology #lectureseries #metabolomics #molecularbiology  #englishstream #msc #phd #livestream #computers #METLIN #pathways #pathwayanalysis #MALDI #chromatography #ionization #electrospray #metabolites