ZINC FINGER NUCLEASES - GENE EDITING EXPLAINED!
Channel: Genomics Guru
Date: 2020-02-18

This series of short presentations on gene editing is brought to you by Dr Adam West, College of Medical Veterinary and Life Sciences at the University of Glasgow, Scotland.
https://www.gla.ac.uk/people/adamwest

This presentation describes the architecture of zinc finger nucleases, which are engineered proteins used for genome editing. 

This is part of a series (see playlist) that also covers
Transcription activator-like effector nucleases (TALENs) 
CRISPR adaptive immune systems
Using CRISPR/Cas9 for genome editing
CRISPR specificity
Precision editing
Homology directed repair
Prime editing

This presentation is primarily aimed at university students, researchers, clinicians and journalists interested in fields related to molecular biology and genetics. It is for education purposes only.

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More accessible presentations for a wide audience can be found in the “Explained Simply” section of this channel in the near future.

Artwork is © Adam West with the exception of images and data taken from publications referenced in these slides. References to reviews and journal articles are denoted by circled numbers at the bottom right of the slides. 

Links to these numbered articles are listed below. We recommend these as your best way to begin further reading on this subject.
Publication List
1. Wah DA, Hirsch JA, Dorner LF, Schildkraut I, Aggarwal AK. 
Structure of the multimodular endonuclease FokI bound to DNA. 
Nature. 1997 Jul 3;388(6637):97-100. 
https://doi.org/10.1038/40446.

2. Wah DA, Bitinaite J, Schildkraut I, Aggarwal AK. 
Structure of FokI has implications for DNA cleavage. 
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10564-9. 
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC27934/

5. Wolfe SA, Greisman HA, Ramm EI, Pabo CO. 
Analysis of zinc fingers optimized via phage display: evaluating the utility of a recognition code. 
J Mol Biol. 1999 Feb 5;285(5):1917-34. 
https://doi.org/10.1006/jmbi.1998.2421

6. Wolfe SA, Nekludova L, Pabo CO. 
DNA recognition by Cys2His2 zinc finger proteins. 
Annu Rev Biophys Biomol Struct. 2000;29:183-212. 
https://doi.org/10.1146/annurev.biophys.29.1.183

7. Klug A. 
The discovery of zinc fingers and their development for practical applications in gene regulation and genome manipulation. 
Q Rev Biophys. 2010 Feb;43(1):1-21. 
https://doi.org/10.1017/S0033583510000089

8. Urnov FD, Rebar EJ, Holmes MC, Zhang HS, Gregory PD. 
Genome editing with engineered zinc finger nucleases. 
Nat Rev Genet. 2010 Sep;11(9):636-46. 
https://doi.org/10.1038/nrg2842

9. Carroll D. 
Genome engineering with zinc-finger nucleases. 
Genetics. 2011 Aug;188(4):773-82. 
https://doi.org/10.1534/genetics.111.131433

10. Kim H, Kim JS. 
A guide to genome engineering with programmable nucleases. 
Nat Rev Genet. 2014 May;15(5):321-34. 
https://doi.org/10.1038/nrg3686

Music credits
“Go, Icarus! Go!” by The Whole Other
“New Year” by Bad Snacks
(YouTube Audio Library)