Hybridisation (molecular biology) | Wikipedia audio article
Channel: wikipedia tts
Date: 2019-05-03

This is an audio version of the Wikipedia Article:
https://en.wikipedia.org/wiki/Nucleic_acid_hybridization


00:01:14 1 Applications
00:02:31 1.1 DNA-DNA hybridization
00:02:43 1.2 Fluorescence In Situ Hybridization



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SUMMARY
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In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA. Though a double-stranded DNA sequence is generally stable under physiological conditions, changing these conditions in the laboratory (generally by raising the surrounding temperature) will cause the molecules to separate into single strands. These strands are complementary to each other but may also be complementary to other sequences present in their surroundings. Lowering the surrounding temperature allows the single-stranded molecules to anneal or “hybridize” to each other.
DNA replication and transcription of DNA into RNA both rely upon nucleotide hybridization, as do molecular biology techniques including Southern blots and Northern blots, the polymerase chain reaction (PCR), and most approaches to DNA sequencing.