Flow cytometry | Wikipedia audio article
Channel: wikipedia tts
Date: 2019-10-02

This is an audio version of the Wikipedia Article:
https://en.wikipedia.org/wiki/Flow_cytometry


00:01:21 1 History
00:03:00 1.1 Name of the technology
00:03:42 2 Flow cytometers
00:06:49 3 Hardware
00:06:57 3.1 Fluidics system of a flow cytometer
00:07:32 3.1.1 Hydrodynamic focusing
00:08:13 3.1.2 Acoustic-assisted hydrodynamic focusing
00:08:48 3.2 Optics and electronics
00:08:57 3.2.1 Optical filters
00:09:39 3.2.2 Prisms, gratings, and spectral flow cytometry
00:10:15 3.2.3 Imaging flow cytometry
00:10:42 4 Data analysis
00:10:52 4.1 Compensation
00:12:43 4.2 Gating
00:13:59 4.3 Computational analysis
00:15:05 4.4 FMO controls
00:15:55 5 Cell sorting by flow cytometry
00:18:04 6 Labels
00:18:40 6.1 Fluorescent labels
00:20:09 6.2 Quantum dots
00:20:25 6.3 Isotope labeling
00:21:14 7 Cytometric bead array
00:22:26 8 Impedance flow cytometry
00:23:21 9 Measurable parameters
00:25:26 10 Applications
00:27:36 10.1 CFSE assay
00:28:59 11 See also
00:29:33 12 Bibliography



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SUMMARY
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Flow cytometry is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles.  A sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument.  The sample is focused to ideally flow one cell at a time through a laser beam and the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so that light is first absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer.
Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. Uses for flow cytometry include:

Cell counting
Cell sorting
Determining cell characteristics and function
Detecting microorganisms
Biomarker detection
Protein engineering detection
Diagnosis of health disorders such as blood cancersA flow cytometry analyzer is an instrument that provides quantifiable data from a sample.  Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties.