Wobble Hypothesis  | Anti-Codon | BIOCHEM| Part-4 | Protein synthesis |
Channel: Basic Science Series 
Date: 22/04/2020

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The Wobble Hypothesis explains why multiple codons can code for a single amino acid. One tRNA molecule (with one amino acid attached) can recognize and bind to more than one codon, due to the less-precise base pairs that can arise between the 3rd base of the codon and the base at the 1st position on the anticodon.

Genetic code: The genetic code is the set of rules by which the information encoded within DNA or RNA sequences is translated into proteins. It consists of a series of codons, which are three-nucleotide sequences that specify a particular amino acid or signal the termination of protein synthesis.

Degeneracy of the genetic code: The genetic code is degenerate, meaning that multiple codons can code for the same amino acid. For example, there are 61 sense codons (codons that code for amino acids) in the genetic code, but only 20 amino acids used in protein synthesis. This degeneracy allows for redundancy and error tolerance in the genetic code.

Codon-anticodon pairing: During translation, transfer RNA (tRNA) molecules bring amino acids to the ribosome, where they are added to the growing polypeptide chain. Each tRNA molecule has an anticodon region that can base pair with the corresponding codon in mRNA.

Wobble position: The Wobble Hypothesis, proposed by Francis Crick in 1966, suggests that the first two nucleotide positions in a codon-anticodon pairing follow strict base-pairing rules (A-U and G-C), but the third position (known as the wobble position) exhibits flexibility in base pairing.

Non-Watson-Crick base pairing: At the wobble position, non-standard base pairs can occur between the anticodon of tRNA and the codon in mRNA. For example, while G-C and A-U base pairs are the standard Watson-Crick base pairs, the wobble position allows G-U base pairs and certain other non-standard pairs.

Expanded codon-anticodon pairing: The wobble position flexibility expands the pairing possibilities of the genetic code, enabling a single tRNA molecule to recognize multiple codons that differ only at the wobble position. This accounts for the degeneracy of the genetic code.

Error minimization: The Wobble Hypothesis helps to minimize the impact of errors during translation. If a mismatch occurs at the wobble position, it is less likely to affect the accuracy of protein synthesis, as the correct amino acid can still be incorporated due to the degeneracy of the genetic code.

Evolutionary implications: The flexibility of the wobble position allows for genetic variation and adaptation. It is thought to have provided an evolutionary advantage by allowing the genetic code to expand and accommodate changes in DNA sequences without altering the amino acid sequence of proteins.

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