World Wide Series | Open Neuroscience - 02/04/2021 - Anton Nikolaev and Pavel Katunin
Channel: Openeuroscience
Date: 2021-04-02

World Wide Science is an ongoing effort to continue, expand and democratise science seminars. Explore our series here: https://www.world-wide.org/Neuro/Open-Source-Neuro/.

Today, we are delighted to host Dr. Anton Nikolaev and Pavel Katunin presenting:

"An open-source experimental framework for automation of cell biology experiments"

Modern biological methods often require a large number of experiments to be conducted. For example, dissecting molecular pathways involved in a variety of biological processes in neurons and non-excitable cells requires high-throughput compound library or RNAi screens. Another example requiring large datasets -  modern data analysis methods such as deep learning. These have been successfully applied to a number of biological and medical questions. In this talk we will describe an open-source platform allowing such experiments  to be automated. The platform consists of an XY stage, perfusion system and an epifluorescent microscope with autofocusing. It is extremely easy to build and can be used for different experimental paradigms, ranging from immunolabeling and routine characterisation of large numbers of cell lines to high-throughput imaging of fluorescent reporters. https://www.biorxiv.org/content/10.1101/2020.07.02.185454v2.full.pdf

Read more about the project here: https://open-neuroscience.com/post/an_open_source_experimental_framework_for_automation_of_high_throughput_cell_biology_experiments/
Get source files here: https://github.com/frescolabs/FrescoM