BigData II: Registration & Stitching of TB image datasets - [NEUBIAS Academy @Home] Webinar
Channel: NEUBIAS
Date: 2021-01-23

Second webinar of the  @NEUBIAS  Academy series about Image Big Data:



Stitching and registration approaches applicable for big data sets.

– BigStitcher enables interactive visualization, fast and precise alignment, spatially resolved quality estimation, real-time fusion and deconvolution of terabyte-sized dual-illumination, multitile, multiview datasets. The software also compensates for optical effects, thereby improving accuracy and enabling subsequent biological analysis.

– MosaicExplorerJ is an IJ macro that enables to explore, interactively align and stitch regular grids of 3D TIFF tiles, e.g. from TB-sized lighsheet microscopy datasets. The whole procedure can be quickly performed and it brings a direct readout on the quality of the alignment. MosaicExplorerJ supports dual-camera detection and dual-illumination sides, and it can compensates for inhomogeneous illumination intensity.

– Using BigWarp to manually transform and export your image data. Example and demo with e.g. CLEM data.

00:00 Intro

00:48 BigStitching (David Hoerl)
47:45 MosaicExplorerJ (Sébastien Tosi) 
1:17:04 BigWarp (John Bogovic)

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Presenters:

David Hoerl, Department of Biology II, Ludwig-Maximilians-University (LMU) in Munich (Germany).
Stephan Preibisch, HHMI Janelia (USA) and group leader at the Berlin Institute for Medical Systems Biology. 

Sébastien Tosi, Advanced Digital Microscopy facility of IRB Barcelona (Spain)

John Bogovic, Stephan Saalfeld’s lab at the HHMI Janelia Research campus (USA)

Moderators: Rocco D’Antuono (CALM, The Francis Crick Institute, UK), Anna Klemm (SciLifeLab, University of Uppsala, SE), Ofra Golani (Weizmann Institute of Science, IL), David Barry (CALM, The Francis Crick Institute, UK),
Julien Colombelli (ADM, IRB Barcelona, ES)

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All Questions and Answers addressed in this webinar are reported in the image.sc forum here:
https://forum.image.sc/t/neubias-academy-home-webinar-image-big-data-ii-registration-stitching-of-tb-datasets-questions-answers/48494

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