2021_01_07  36hr peek at Acute Rapid Toxicity Screening - WRD PFAS _ AO
Channel: Drone File Drop
Date: 2023-01-08

A peek to see how an aqueous L. sativa tox assay is doing after 36 hours.

Growth in control samples is up over 5cm already in some seeds.
There is budding in some of the site samples, indicating that there may not
be tox effects. 

If there is absolutely no budding within 3- 5 days with Site samples, and you have good budding and growth in Controls, that is a qualitative indication that there may be toxicity associated with your Site.

A root heating mat, set at 70 F is used.  The Acute Rapid Toxicity Screening (ARTS) seed test is run in triplicate, 10 seeds per cup, 3 ml of sample, 
for up to 21 days. 

Germination and Root Length are quantified as measures of potential Site Toxicity as compared to Controls.  If toxicity is observed in the ARTS test, this could warrant the request for Standardized Toxicity Testing.

AO
Jan 07, 2022

ostaszewskia@michigan.gov