Very Quick and imperfect Tutorial on ImageJ
Channel: Bad Idea
Date: 2021-03-10

Better go to the more convenient tutorial here:
https://youtu.be/zCUY66ttiaU

Everything below is related to that video (https://youtu.be/zCUY66ttiaU)
Here is the link to images I was using in the video:
https://drive.google.com/drive/folders/1jL8dQrnIYtjVU6BQSxFSQHr0NQeRMQq0?usp=sharing

In this video I am introducing you to ImageJ-based pack Fiji. 

I'm going to show the plot profile of the gel bands, merging and splitting of the channels, working with different channels and enhancing the images, removal of background. 
Also you will see how to apply 3D Project from the stacks of previously taken z-stack microscopy slicing. For that you will need to determine the steps of microscope's fine knob. You define it by counting the thickness of cover slips in scale interval of the fine knob. Usual high quality coverslips have shown their physical thickness on the box. After you determine the value of the scale intervals you could create the 3D project and set the appropriate slice thickness. 
Finally we talk about the tracking of vesicles and how to deal with compact, numerous stacks. It can be handled through the hyper-stack formation. 

Also you will see how to apply the macros and try it out. I don't show the script writing since it should be done based on the particular task / problem.

Leave comments if something should be explained in more details or I am mistaken in the explanation, etc

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