Alesia Lokshina ORS2022 Gaussia Luciferase Assay Stabilization for Analysis of Chondrogenesis
Channel: Cell and Tissue Engineering Lab
Date: 2022-02-05

Gaussia luciferase typically exhibits a flash reaction. This enzyme is a commonly used reporter that we have developed for the assessment of chondrogenesis under the control of the type II collagen promoter. We tested several buffers to stabilize the signal to make it more amenable for high-throughput assays and to reduce plate to plate variability when a direct injector on the plate reader is unavailable.