Sample Fixation
Channel: Zbigniew Mikulski
Date: 2023-11-12

Zbigniew Mikulski discusses his favorite subject- proper Sample Fixation, which is absolutely essential to all tissue-based work.

00:00  Need for proper sample fixation by Zbigniew Mikulski
01:52  Limitations of formalin as a fixative
4:50 (Para)Formaldehyde and formalin
6:28 Recommendations for good fixation 
10:35 How to recognize poor fixation in your tissues?
13:10 Alternatives to FFPE samples
14:32 The art of histology
16:05 Examples of underfixed tissue blocks and slides
17:50 FFPE tissue samples used for STED imaging
18:20 Iterative improvements in sample preparation, imaging, and analysis

If you have any questions about the videos or the training course, please ask on this image.sc forum post. https://forum.image.sc/t/qupath-training-course-from-samples-to-knowledge-2023-is-available-online/88618
The more information you can include about your question, the more helpful our answers can be.  

Please follow this document to get everything you need (software, files, cheat sheets, and additional resources):
https://docs.google.com/document/d/1VyPtSbvnUC3EWnCVSRmFgFnhsXNHfOkAxOVyhkpYn8I