Tarek Obeid presenting his #ORS2024 poster on the effect of DRD4 knockout on chondrogenesis
Channel: Cell and Tissue Engineering Lab
Date: 2024-02-02

We recently discovered that aromoline stimulates type II collagen production from human articular chondrocytes. We further identified that its putative target is the dopamine receptor D4. This was a novel finding and the presence of this G-protein receptor in cartilage has an unknown role. After confirming the presence of the receptor by both immunocytochemistry and qPCR we decided to knock out the receptor in both primary human chondrocytes and immortalized human chondrocytes (TC28A2). We then studied the effect of knockout on chondrogenesis in 3D culture.