ESMARConf2022 Special Session 5: Other quantitative synthesis - Maria Llambrich
Channel: ESMARConf
Date: 23/02/2022

Presenter: Maria Llambrich
Session: Special Session 5: Other quantitative synthesis
Title: A new approach for meta-analysis using overall results: Amanida
Abstract: The combination, analysis and evaluation of different studies which try to answer or solve the same scientific question, also known as a meta-analysis, plays a crucial role in answering relevant clinical relevant questions. Unfortunately, metabolomics studies rarely disclose all the statistical information needed to perform a meta-analysis in a traditional manner. Public meta-analysis tools can only by applied to data with standard deviation or directly to raw data. Currently there is no available methodology to do a meta-analysis based on studies that only disclose overall results. Here we present Amanida as a meta-analysis approach using only the most reported statistical parameters in this field: p-value and fold-change. The p-values are combined via Fisher’s method and fold-changes are combined by averaging, both weighted by the study size (n). The amanida package includes several visualization options: a volcano plot for quantitative results, a vote plot for total regulation behaviors (up/down regulations) for each compound, and a explore plot of the vote-counting results with the number of times a compound is found upregulated or downregulated. In this way, it is very easy to detect discrepancies between studies at a first glance.  Now we have developed a Shiny app to perform meta-analysis using Amanida approach and make it more accessible for the community. 

Please cite as: Llambrich, Maria (2022) A new approach for meta-analysis using overall results: Amanida. ESMARConf2022 conference presentation. Zenodo. https://doi.org/10.5281/zenodo.6079610