6. Green Listed - How to design a Non-targeting control library
Channel: Fredrik Wermeling
Date: 18/10/2016

See https://wermelinglab.com/green-listed/ for more information.

Green Listed is a tool facilitating the rapid design of custom CRISPR screens. It is used to mine publically available source lists of gRNAs to design custom libraries targeting genes specified by the user, such as all kinases or all genes up-regulated in an RNAseq experiment.

To facilitate cloning work with the identified gRNA sequences, the software can easily modify the output sequences by trimming the 5’ and/or 3’ end of the sequence, as well as by adding adaptor sequences. Specific sequences can also be identified in the list of gRNAs to help the user to identify gRNAs containing sequences, such as restriction sites, not compatible with downstream applications. Furthermore, if the uploaded source list (the list of gRNAs) contains any type of numerical ranking system, ranking can be applied enabling the user to select gRNAs with various properties.

This project was financially supported by the Swedish Research Council, the Swedish Foundation for Strategic Research, the Wenner-Gren Foundations, the Karolinska Institutet and BILS (National Bioinformatics Infrastructure for Life Sciences).

Green Listed is for non-profit and academic use only. All commercial rights are reserved.

© 2016 – Green Listed