Creating informative western blot figures - ReproducibiliTeach
Channel: ReproducibiliTeach
Date: 2022-10-18

Learn how to make western blot figures that give readers the information needed to fully evaluate your data. Topics include the importance of cropping the blot as little as possible, depositing full plots (source data) on a data repository, ensuring that the blot includes a visible molecular weight maker, including molecular weight labels for all marker bands, and ensuring that the blot background is gray (clearly distinguishable from the white page). Viewers will learn why each of these recommendations are important for transparency and reproducibility.

See our recent paper to learn more about these recommendations, and the data underlying them. The paper also includes data and recommendations on how to report your western blot methods reproducibly.
https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3001783