Ram Medam ORS2022 Promoting Type II Collagen in Tissue Engineered Human Cartilage via DoE
Channel: Cell and Tissue Engineering Lab
Date: 2022-02-05

Tissue engineered human cartilage is typically deficient in type II collagen. We created a human chondrocyte reporter to optimize conditions for type II collagen expression. This human chondrocyte reporter was used in a Design of Experiment based approach to identify conditions for optimal differentiation of human chondrocytes. DMEM, the basal media for most defined chondrogenesis experiments, lacks 14 vitamins and minerals required physiologically. The combinatorial effect of those vitamins and minerals along with a comparison between DMEM and DMEM/F12 as basal medias was performed. DMEM/F12 significantly increased metabolic activity but not type II collagen expression.