Cryopreservation | Wikipedia audio article
Channel: wikipedia tts
Date: 06/05/2019

This is an audio version of the Wikipedia Article:
https://en.wikipedia.org/wiki/Cryopreservation


00:01:27 1 Natural cryopreservation
00:03:44 2 History
00:05:50 3 Temperature
00:07:14 4 Risks
00:08:59 5 Main methods to prevent risks
00:09:23 5.1 Slow programmable freezing
00:11:59 5.2 Vitrification
00:15:18 6 Freezable tissues
00:18:42 6.1 Embryos
00:20:00 6.2 Ovarian tissue
00:21:03 6.3 Oocytes
00:22:16 6.4 Semen
00:22:53 6.5 Testicular tissue
00:23:38 6.6 Moss
00:24:06 6.7 Mesenchymal stromal cells (MSCs)
00:25:05 7 Preservation of microbiology cultures
00:25:53 7.1 Fungi
00:27:27 7.2 Bacteria
00:29:07 7.3 Worms
00:29:37 8 See also



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SUMMARY
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Cryo-preservation or cryo-conservation is a process where organelles, cells, tissues, extracellular matrix, organs, or any other biological constructs susceptible to damage caused by unregulated chemical kinetics are preserved by cooling to very low temperatures (typically −80 °C using solid carbon dioxide or −196 °C using liquid nitrogen). At low enough temperatures, any enzymatic or chemical activity which might cause damage to the biological material in question is effectively stopped. Cryopreservation methods seek to reach low temperatures without causing additional damage caused by the formation of ice crystals during freezing. Traditional cryopreservation has relied on coating the material to be frozen with a class of molecules termed cryoprotectants. New methods are constantly being investigated due to the inherent toxicity of many cryoprotectants. By default it should be considered that cryopreservation alters or compromises  the structure and function of cells unless it is proven otherwise for a particular cell population. Cryoconservation of animal genetic resources is the process in which animal genetic material is collected and stored with the intention of conservation of the breed.