HPLC CHROMATOGRAPHY II NORMAL PHASE HPLC II REVERSE PHASE HPLC II NEET II CSIR II DBT II ICMR IIGATE
Channel: DEEPAK BIOLOGY CLASSES AND JOBS TELLER
Date: 08/05/2020

This video shows about the HPLC principles, componenets of HPLC, types of HPLC i.e. Normal phase HPLC and Reverse phase HPLC. Rverse phase HPLC is widely used in pharma industry, analytical chemistry, forensic sciences, clinical sciences because of good resolution and sensitivity.
The principle includes two phases i.e. mobile phase and stationary phase and the interaction between these two phases results in separation of componenets from the mixture.
In NP-HPLC include non-polar mobile phase and polar stationary phase while in RP-HPLC include just reverse of NP-HPLC i.e. polar mobile phase and non-polar stationary phase.
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