Read alignment (3 of 5)
Channel: SIB - Swiss Institute of Bioinformatics
Date: 2023-01-16

The video was recorded live during the SIB course “NGS - Quality control, Alignment, Visualisation” streamed on 21-23 November 2022. 

It introduces you to the most used sequencing technologies and explain their concepts. Using different datasets, we will practice quality control, alignment of reads to a reference genome and visualize the output. 

The course materials can be found in the dedicated GitHub page: https://sib-swiss.github.io/NGS-introduction-training/ . 

This video contains information on how to: 

- Explain what a sequence aligner does 
- Explain why in some cases the aligner needs to be ‘splice-aware’ 
- Calculate mapping quality out of the probability that a mapping position is wrong 
- Build an index of the reference and perform an alignment of paired-end reads with bowtie2 

Target audience: PhD students, post-docs and researchers in life sciences who are doing bioinformatic analyses and want to get introduced to containers. 

Speaker: Dr. Geert van Geest, SIB Swiss Institute of Bioinformatics 

Any questions about this talk? Contact: Geert van Geest, Geert.Vangeest@sib.swiss