Complementary DNA | Wikipedia audio article
Channel: wikipedia tts
Date: 2019-01-13

This is an audio version of the Wikipedia Article:
https://en.wikipedia.org/wiki/Complementary_DNA


00:01:22 1 Synthesis
00:04:24 2 Applications
00:06:38 3 Viruses
00:07:39 4 See also



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SUMMARY
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In genetics, complementary DNA (cDNA) is DNA synthesized from a single-stranded RNA (e.g., messenger RNA (mRNA) or microRNA) template in a reaction catalyzed by the enzyme reverse transcriptase. cDNA is often used to clone eukaryotic genes in prokaryotes.  When scientists want to express a specific protein in a cell that does not normally express that protein (i.e., heterologous expression), they will transfer the cDNA that codes for the protein to the recipient cell.  cDNA is also produced naturally by retroviruses (such as HIV-1, HIV-2, simian immunodeficiency virus, etc.) and then integrated into the host's genome, where it creates a provirus.The term cDNA is also used, typically in a bioinformatics context, to refer to an mRNA transcript's sequence, expressed as DNA bases (GCAT) rather than RNA bases (GCAU).
cDNA is derived from mRNA, so it contains only exons but no introns.