Protein identification and structure (Bioinformatics S5E3)
Channel: Danny Arends
Date: 30/11/2021

Learn about protein identification through Mass Spectrometry and Nuclear magnetic resonance (NMR). Predict protein structure by homology modelling, AlphaFold, and X-ray crystallography. Learn about protein properties like isoelectric points and how protein are classified by function.

Lectures @ https://dannyarends.nl/bioinformatik/
Programming Assignments and Answers @ https://dannyarends.nl/bioinfo/
Playlist: https://www.youtube.com/playlist?list=PLhR2Go-lh6X5A5WbiO3SPHuoWbwpNznUl

Chapters:
0:00 Welcome
0:32 Protein structure prediction by fold recognition or homology modelling
2:14 Protein structure prediction by Google AlphaFold
4:00 The universal genetic code and production of proteins
5:18 Redundancy in the universal genetic code
7:06 The wobble base - Third base degeneracy
10:25 The amino acid codonwheel and IUPAC
13:30 Some words about the universal genetic code
16:20 Protein identification and purification
17:03 Protein separation by Ultracentrifugation
18:17 Protein separation by Precipitation
19:52 Protein separation by Electrophoresis
21:08 The isoelectric point of a protein
23:32 Protein separation by 2D Gel electrophoresis
25:16 Mixture separation by chromatography
27:46 Summary of protein separation/purification techniques
28:33 Immunohistochemical identification of proteins
30:23 Molecular structure of proteins by X-ray crystallography
32:07 Molecular structure of proteins by Nuclear magnetic resonance (NMR)
33:15 Protein identification by Mass Spectrometry 
35:56 Summary of protein identification techniques
37:06 Protein classification by function
40:25 Protein domains determine protein function
42:03 Protein families
44:53 Software tools and databases to investigate proteins

This is a live-stream recording of the MSc and PhD lecture series: Bioinformatics for plant and animal sciences, given digitally during the Covid19 pandemic at the Humboldt University in Berlin organized and lectured by Dr Danny Arends.

Thanks for taking an interest in my channel 😄If you've made it this far down, support me by giving a like or subscribing. Join me during my live streams Thursday afternoons on Twitch @ https://www.twitch.tv/dannyarends

This work is licensed under version 3.0 of the Creative Commons CC-BY license. 
For more information see http://creativecommons.org/licenses/by/3.0/legalcode

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