CH450 Chapter 3.1 Protein Purification
Channel: Patricia Flatt
Date: 2020-09-23

This section details common methods used in biochemistry to isolate and purify proteins from complex mixtures. It includes discussions about salting in and salting out, density gradient centrifugation, and several different chromatography techniques. Chromatography covered includes: Size Exclusion Chromatography (Gel Filtration Chromatography); Hydrophobic Interaction Chromatography (HIC); Ion Exchange Chromatography, and Affinity Chromatography. Gravity fed column,  HPLC and FPLC are also discussed.