Microgenomics 2021 webinar:  Single-cell analysis as a promising technique for plant disease
Channel: The International Symposium on Microgenomics 2023
Date: 2022-01-25

Nicola Luchi (National Research Council, Italy): "Single-cell analysis as a promising technique for plant disease"

Over the last 20 years, biosecurity protocols for plant protection have been developed in order to prevent the introduction, diffusion and eradication of invasive plant pathogens. Ceratocystis platani is an invasive fungal pathogens threatening plane trees (Platanus), causing rapid death of the plant by blocking the vascular system. Recently, in the study of blood cancer disease new innovative approaches, based on single-cell have, been developed and they can be easily and successfully transferred to plant wilt pathogens.

In the present work the DEPArray was able to recovery single C. platani conidia or small group of conidia in single microtubes. After the whole genome amplification, the identification of C. platani DNA was performed by amplification and sequencing of ITS target region by using a specific real-time PCR markers. Sequences analysis confirms the presence of C. platani target qPCR amplicon detected in a single conidia.

The use of single-cell will provide new insight in plant pathology especially in the studies of vascular disease.