04R Preserve in glycerol  Alizarin Red Staining, Step-by-step guide:  2023 1130 04 RedD
Channel: Laboratory of Aquatic Zoology
Date: 14/12/2023

This is how goldfish skeleton specimens are made.

Remove skin:  https://youtu.be/XKZR_x7p7FY?si=x8nB6vHXHVz9kKUR
Staining: https://youtu.be/Mrg3UqqLdFs
Soaking in water:  https://youtu.be/a88RGWn1ZOo
Recipe of Scale A2:   https://youtu.be/LNAD5GVTyII
Preserve in glycerol: https://youtu.be/3DkBUCpTVdM

Safety First:
Do not drink or consume any of the reagents. Our experiments involve substances that are not intended for ingestion. Use protective equipment, like gloves. Safety is our top priority, so let's keep those hands safe! Work in a well-ventilated area. Ensure proper airflow to minimize exposure to fumes. Read and follow safety guidelines for each reagent. Knowledge is key to a safe experiment!

References:
Our works:
Li, Ing Jia, Chun Ju Chang, Shi Chieh Liu, Gembu Abe, and Kinya G. Ota. “Postembryonic Staging of Wild-Type Goldfish, with Brief Reference to Skeletal Systems.” Developmental Dynamics 244, no. 12 (2015): 1485–1518. https://doi.org/10.1002/dvdy.24340.
Li, Ing-Jia, Shu-Hua Lee, Gembu Abe, and Kinya G. Ota. “Embryonic and Post-Embryonic Development of the Ornamental Twin-Tail Goldfish.” Developmental Dynamics, no. 23 (2019): 1–33. https://doi.org/10.1002/dvdy.15.
Ota, Kinya G. “Experimental Notes.” In Goldfish Development and Evolution, 249–79. Springer, Singapore, 2021.


Original article of Scale A2:
Hama, Hiroshi, Hiroshi Kurokawa, Hiroyuki Kawano, Ryoko Ando, Tomomi Shimogori, Hisayori Noda, Kiyoko Fukami, Asako Sakaue-Sawano, and Atsushi Miyawaki. “Scale: A Chemical Approach for Fluorescence Imaging and Reconstruction of Transparent Mouse Brain.” Nature Neuroscience 14, no. 11 (2011): 1481–88. https://doi.org/10.1038/nn.2928.




#透明標本
#goldfish research 
#teleost fish 
#biology 
#Diaphonization

#Kinya Ota
#太田欽也 
#宜蘭 
#taiwan 
#yılan 
#biology 
#Marine Research Station