Sean Kennedy ORS2022 Development of Human Reporter Chondrocytes for SOX9 and PRG4
Channel: Cell and Tissue Engineering Lab
Date: 2022-02-05

SOX9 is commonly regarded as the master transcription factor for cartilage development. PRG4 or lubricin is an essential proteoglycan that lubricates the surface of articular cartilage. We have previously developed type II collagen human chondrocyte reporter cells using the Gaussia luciferase reporter. We used this same reporter under the control of SOX9- and PRG4-promoters. Correlation between the luciferase enzyme expression and each gene was established, along with evidence of expression under the control of TGFb1. Type II collagen optimized media were tested under atmospheric and physioxic conditions.