How to Analyze Annexin-V Apoptosis Assay Data Using Flow Cytometer ?
Channel: Bio-Capsule
Date: 2022-12-25

Data analysis for the Annexin-V Assay is a difficult skill for beginners to learn. I spent several hours during my earlier Ph.D. career learning these skills, and I have a very hard time finding the right resources to receive direction. I covered the following approaches to simplifying it in this video.
1. How to  open data in the Max Quanta Analyzer software
2. How to plot different types of FACS data, in software?
3. How to analyze Annexin V data in Max Quanta analyzer 10?
4. How to perform quantitative analysis of Apoptosis data?

What is compensation?
Instrument compensation is crucial for the optimal acquisition and display of data obtained by flow cytometry. Compensation essentially accounts for the inherent overlap in emission spectra observed between different fluorochromes. This fluorescence spectral overlap, or ‘spillover’, will result in the detection of individual fluorochromes in more than one fluorescence channel. Spillover must be determined and corrected. This is especially important when multicolor analyses are performed—without proper compensation, results may be misinterpreted.

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