MIHIC: a multiplex IHC histopathological image classification dataset for lung cancer immune microenvironment quantification

Authors:
raw_author_name: Ranran Wang
raw_affiliation_string: Affiliated Cancer Hospital, Dalian University of Technology, Dalian, China; School of Biomedical Engineering, Faculty of Medicine, Dalian University of Technology, Dalian, China
raw_author_name: Tong Wang
raw_affiliation_string: School of Biomedical Engineering, Faculty of Medicine, Dalian University of Technology, Dalian, China
raw_author_name: Shan Jin
raw_affiliation_string: School of Biomedical Engineering, Faculty of Medicine, Dalian University of Technology, Dalian, China
raw_author_name: Fengyu Cong
raw_affiliation_string: Affiliated Cancer Hospital, Dalian University of Technology, Dalian, China; Faculty of Information Technology, University of Jyvaskyla, Jyvaskyla, Finland; Key Laboratory of Integrated Circuit and Biomedical Electronic System, Dalian University of Technology, Dalian, Liaoning, China; School of Biomedical Engineering, Faculty of Medicine, Dalian University of Technology, Dalian, China
raw_author_name: Yong Zhang
raw_affiliation_string: Department of Pathology, Liaoning Cancer Hospital and Institute, Shenyang, China
raw_author_name: Hongming Xu
raw_affiliation_string: Affiliated Cancer Hospital, Dalian University of Technology, Dalian, China; Key Laboratory of Integrated Circuit and Biomedical Electronic System, Dalian University of Technology, Dalian, Liaoning, China; School of Biomedical Engineering, Faculty of Medicine, Dalian University of Technology, Dalian, China

Abstract: Background Immunohistochemistry (IHC) is a widely used laboratory technique for cancer diagnosis, which selectively binds specific antibodies to target proteins in tissue samples and then makes the bound proteins visible through chemical staining. Deep learning approaches have the potential to be employed in quantifying tumor immune micro-environment (TIME) in digitized IHC histological slides. However, it lacks of publicly available IHC datasets explicitly collected for the in-depth TIME analysis. Method In this paper, a notable Multiplex IHC Histopathological Image Classification (MIHIC) dataset is created based on manual annotations by pathologists, which is publicly available for exploring deep learning models to quantify variables associated with the TIME in lung cancer. The MIHIC dataset comprises of totally 309,698 multiplex IHC stained histological image patches, encompassing seven distinct tissue types: Alveoli, Immune cells, Necrosis, Stroma, Tumor, Other and Background. By using the MIHIC dataset, we conduct a series of experiments that utilize both convolutional neural networks (CNNs) and transformer models to benchmark IHC stained histological image classifications. We finally quantify lung cancer immune microenvironment variables by using the top-performing model on tissue microarray (TMA) cores, which are subsequently used to predict patients’ survival outcomes. Result Experiments show that transformer models tend to provide slightly better performances than CNN models in histological image classifications, although both types of models provide the highest accuracy of 0.811 on the testing dataset in MIHIC. The automatically quantified TIME variables, which reflect proportions of immune cells over stroma and tumor over tissue core, show prognostic value for overall survival of lung cancer patients. Conclusion To the best of our knowledge, MIHIC is the first publicly available lung cancer IHC histopathological dataset that includes images with 12 different IHC stains, meticulously annotated by multiple pathologists across 7 distinct categories. This dataset holds significant potential for researchers to explore novel techniques for quantifying the TIME and advancing our understanding of the interactions between the immune system and tumors.