RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Molecular characterisation of the wound-response in plants. An approach based on gene activation by t-dna tagging' under Fourth Framework.

assistant: **Concept**

Plants react to wounding by transcriptionally activating specific defence-related genes. In this project we will develop an approach based on gene activation by T-DNA tagging in order to isolate genes which are regulatory steps for defence-gene expression. This technique is based on the ability of the T-DNA, derivating from the Agrobacterium Ti plasmid, to randomly integrates into plant genome. Activation of tagged genes is achieved by using a T-DNA tagging vector which contains four CaMV 35S transcriptional enhancers. In order to isolate wound-regulatory genes, tomato transgenic lines carrying wound-inducible promoters fused to a marker gene, luciferase or bar (selection on the herbicide basta) genes, will be obtained. Activation tagging will be carried out on leaf protoplasts isolated from these marker lines. Screening of mutants will be done by recording luciferase activity or selection for basta resistance. Tagged genes will be characterised.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Structural insight into auto-ubiquitylation as a regulatory mechanism for protein trafficking' in the context of a Seventh Framework call.

assistant: **Objectives**

The cell interprets the information conferred by ubiquitin (Ub) through Ub-receptors, proteins that harbor ubiquitin-binding domains (UBDs). Ub-receptors can be found in three mutually exclusive forms. Apo is the unbound form, whereas trans is the intermolecular form bound to ubiquitylated cargo. Intriguingly, the third form, cis, is of Ub-receptors that are themselves ubiquitylated. Monoubiquitylation of the Ub-receptors imposes an auto-inhibitory conformation, rendering them unable to bind ubiquitylated cargos, and thus providing an intrinsic regulatory mechanism. While several structures of apo and trans are available, a cis structure form of an ubiquitylated Ub-receptor has yet to be determined. Determining a cis structure is crucial for understanding the molecular mechanism of auto-inhibition. Due to the action of deubiquitylating enzymes, the ubiquitylated form of the Ub-receptors is labile and therefore it is difficult to purify them from eukaryotes. To fill this gap in knowledge, we developed a novel bacterial system for protein ubiquitylation. The system produces a high yield of ubiquitylated protein that can be easily purified and subjected to crystallization trials. Using this bacterial ubiquitylation system, we will determine cis as well as apo and trans structures of Ub-receptors. Guided by comparison of the structures, point mutations will be introduced into key residues. In vitro functional studies including the determination of oligomeric stages, and affinity measurements using isothermal titration calorimetry and analytical ultracentrifugation will be performed on all three forms. Finally, the phenotype of the wild type and mutant proteins will be studied in vivo by monitoring trafficking of GFP-cargo under confocal microscopy. The outcome of this study
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Role of p63 and related pathways in epithelial stem cell proliferation and differentiation and in rare EEC-related syndromes.' demonstrating excellence, impact and implementation quality for a Sixth Framework call.

assistant: **Abstract**

The focus of 'EPISTEM' is to generate new knowledge and translate it into applications that enhance human health. To this end both fundamental and applied research will be involved. 'EPISTEM' integrates multidisciplinary and coordinated efforts to understand the molecular basis of factors involved in epidermal stem cell generation, maintenance and differentiation and skin disease. Moreover, the core molecule that will be studied in this IP is p63 (and related pathways), a molecule genetically proven to be involved in the development of rare skin diseases such as EEC syndrome, Hay-Wells (AEC) syndrome, Limb-mammary syndrome, ADULT syndrome, Rapp-Hodgkin syndrome and non-syndromic split hand-split foot malformation. Collectively, the prevalence of ectodermal dysplasia syndromes (EDS) is estimated at 7 cases in 10,000 births. Currently there is no cure for these patients. By creating the 'EPISTEM' consortium we want to address from different angles (genetics, gene profiling, molecular and cellular biology, structural biology, drug design, bioinformatics), the molecular pathways involved in epidermal dysplasia syndromes making use of different technologies (mutation analysis, micro-array, ChiP, transgenes, proteomics, in vitro skin cultures, crystallography, etc). Our consortium brings together leading European clinicians, geneticists, molecular and cellular biologists, structural biologists, a drug designer and bioinformatics specialist in the field of p63 (and related molecules) research. Therefore, this research fits in the centre of the specific topic Exploring the potential of stem cells and/or primary cells for the understanding of monogenic rare diseases and the development of new drugs for their treatments"."
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Developing Smart Technologies for Productivity Improvement of European Small and Medium Sized Shipyards' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

The SMARTYards project aims to improve the productivity of European small and medium sized shipyards and related subcontractors working with them by at least 20%. This will be achieved by improving knowledge and technological skills, needed to survive in a tough global competition and to provide innovative products.  This is particularly important as the technology gap between larger and smaller European yards has increased significantly over the last decades.In the technical part, the project will develop, test and validate smart technology solutions, comprising the optimum between design, equipment and work organization. All solutions shall address the specific needs and challenges of the target group, which have been clearly identified in the proposal. Seven key Technology Areas and related innovative ideas have been identified, which represent critical processes from design through production to repair and conversion. These solutions will be adapted and documented in a Technology Catalogue. The most promising solutions will be selected to build, test and validate at least six physical mock-ups, which will be demonstrated to the European maritime community, represented by a User Group. Technical developments will be accompanied by the elaboration of innovative business models, productivity and environmental assessment, the development of training schemes and a sustainable innovation network in close cooperation with European associations.The project comprises 17 partners from 9 European countries with SME shipyards and technology providers in a leading role.While improving the technology level of small and medium sized companies, the project will contribute to the overall competitiveness of the EU maritime sector, sustaining the wide network of large and smaller comp
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Co-Creating Positive and Sustainable Lifestyle Tool with and for European Citizens' (PSLifestyle).

assistant: **Innovation**

Through our consumption behaviour we, individuals, are responsible for 72 per cent of the global greenhouse gas emissions. These are created by the way we live, travel, and eat and by what we buy. So far, the attempts to change people's habits by appealing to their rationale have not produced significant behaviour change.Aiming to help close the action gap between climate awareness and individual action, and to increase citizen participation in sustainability topics, the project builds a data-driven momentum for sustainable behaviour change across eight European countries. It does this by engaging citizens through a digital application to co-research, co-develop and uptake everyday life solutions for climate change, providing tools for the collection, monitoring and analysis of their environmental and consumption data.In the application, the citizens find out how their lifestyle and habits impact the environment. They will also see a list of smart everyday actions that are relevant to reduce their environmental impact. The application enables citizens to actively create data while monitoring their lifestyle induced climate impact and behaviour. The collected data will be used to improve the application itself, and, importantly to empower citizens to gain agency in research and policy-making.The ambition is to engage a total of four million EU citizens. The figure is based on the previous national success with a web-based carbon emission calculator, which is innovated further with the use of citizen science, by co-research at living labs, co-creating personalized sustainability plans, as well as with the integration of behaviour science knowledge into the co-research and design process.The overarching goal of PSLifeStyle is to co-create a web- and mobile-based sustainabl
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Establishing services enhancing the innovation management capacity of SMEs by the Enterprise Europe Network in North Rhine-Westphalia, Germany, in 2017 -2018' in the context of a Horizon 2020 call.

assistant: **Objectives**

This project is to be understood as an integrated component of the Enterprise Europe Network activities in North Rhine-Westphalia for the years 2017 -2018. For this period ZENIT will undertake - as part of the future NRW.Europa project - the additional task of ""Enhancing the innovation manangement capacities of SMEs"".This task consists of two service packages pre-defined by the European Commission / EASME and foreseen for a limited number of enterprises:1. Beneficiaries of the SME Instrument of Horizon 2020 (Key Account Management, KAM)2. SMEs with significant innovation activities and a high potential for internationalization (promising SMEs with gaps in the innovation management systems).Whereas the first action is reserved for beneficiaries of the new SME instrument with a dedicated coaching scheme, the second is targeted at highly promising innovative enterprises, which will be assisted with a two-step support scheme:1. Recording, analysis and evaluation of the individual status quo and of fundamental requirements and possibilities as well as of the threats and risks of the participating enterprises (analysis of needs and gaps, SWOT analysis).2. Development and implementation of individual concepts, measures, and activities (action and / or coaching plan).ZENIT considers a combination of self-assessment and interview with a senior consultant to be the most effective solution for the first stage and to fulfil the requirements of CEN / TS 16555-1 (innovation management system). The ZENIT Self Assessment tool will be used as priority, other tools like IMP³rove, Steinbeis or N-Bank will be introduced to meet specific needs of SMEs.""


**Keywords:** Two Consultancy Services for SMES: a) Key Account Management for SMEs participating at the, SME-instrument, b) Innovatio
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'The world’s “First and Cleverest Space-Based ULD-Tracker” for Airports and Airfreights in EU and worldwide.' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

Main Task is the development and commercialization of STAP ULD-Watcher, a RFID and satellite-based chip to enable worldwirde and real-time localization of ULDs where no additional infrastzructure is needed.The core of STAP is a technology which is in line with IATA air cargo regulations. The value chain includes various groups: airports (implementation partners), airfreight companies (short term customers), transport organizations (customers), forwarding companies (potential supporter of the technology), ground service companies at airports (crucial partners for installation, service and proof of concept), manufacturer of ULD transport vehicles and ULD lifters (potential strategic partners) and second source service provider which are working with tracking data (potential strategic partners). The main customer benefits and USPs of STAP-Watcher are the user friendliness (unique and simple plug-in-and-play system working both indoor and outdoor), highly accurate monitoring as well as the unmatched price (30% cheaper than any closest competitor product in market), which offers a unique return on investment time of 1.2 -2 years, depending on the size and special needs of a specific airport. Further advantages over state-of-the-art systems include small system size, equipped additional sensors, fully automatic documentation (designed for very small to extremely large airport & cargos), no need of addition infrastructure and smart control through actual AT-patented technology. A global PCT is filed in September-2019 to cover whole EU and some core countries worldwide. The airlines and cargo forwarder including ground handlers save upto 16% time and labour costs using STAP watcher compared to currently used state-of-the-art technologies. Furthermore, our open protocol interfac
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Non-histone protein acetylation targets of KAT2A in AML' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

The importance of an insight into how cell fate is established and maintained, extends far beyond the interests of developmental biology. It is pivotal to our understanding of how these processes can be hijacked and deranged in diseases such as cancer, or of the factors involved in reprogramming cell identity and function. Acute Myeloid Leukaemia (AML) has a dismal prognosis with less than 30% 5-year survival. Mainstay therapy has remained essentially unchanged for the past three decades, with all small advances in disease-free survival attributable to transplantation and improved supportive care. Only recently PKC412 has received Food and Drug Administration (FDA)’s break through therapy designation for the FLT3-ITD+ AML. This paves the way for  investigation considering that FLT3-ITD as a driving oncogenic mutation has been found in ~30% of the AML patients. The pathogenesis of AML is heterogeneous, but there are common themes of epigenetic, transcriptional and signalling dysregulation that contribute to the resulting clonal expansion of blasts at different stages of maturation, and accompanying bone marrow failure. A significant number of the most commonly mutated targets in AML are histone modifiers, i.e. proteins or complexes that catalyse post-translational modifications in specific residues of the histone side chains. A less studied acetyltransferase, but crucially implicated in AML is KAT2A, the first histone acetyl-transferase (HAT) identified in yeast.GCN5 also acetylates the AML1/MDS1/EVI1 fusion protein in rare cases of AML.KAT2A regulates the activity of Peroxisome Proliferator- Activated Receptor Gamma-Coactivator-1α and B through protein acetylation. The goal of this proposal is to explore the role of KAT2A in Acute Myeloid Leukaemia (AML) through investi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Visualisation of the exposure of cyclists to traffic on roads' to be submitted under Sixth Framework.

assistant: **Abstract**

The still growing urban traffic intensity in many European cities strongly stimulates bicycling as alternative way of transportation. In its own, bicycling is considered relatively fast, cheap, healthy and good for the environment. Unfortunately it can also be unhealthy as cyclists often suffer from intense exposure to traffic exhaust. However, the actual exposure level of the cyclist is not explicitly documented (the average air-concentration serves as indication) and communication about the hazards involved is limited. As a consequence, cyclist interest groups have a hard time pressing policy makers to take measures to reduce the exposure. VECTOR (Visualisation of the Exposure of Cyclists in Traffic on Roads) will improve this situation by assessing the actual exposure of cyclists throughout Europe (i.e. in NL, DE, HU and LT) and by using combined analysis/video-recording measurements to improve the awareness raising potential of the data. VECTOR will assess the exposure of cyclist by studying the fine particle air-concentration (PMx) at the position of the cyclist on its bicycle. Hereby, a so-called ?measurement bike? is used that was specially developed for such a purpose in a previous initiative by the Dutch Cyclists Association (the Fietsersbond). Combined with the state-of-the-art ?real time visualisation? (RTV) experience of the Kooperationsstelle (Germany), actual exposure videos will be made on the exposure in various traffic situations. These will subsequently be used to inform (local) authorities, cyclist interest groups and other stakeholders about the risks of exposure of cyclists to traffic exhaust. Especially also spreading knowledge about RTV as a tool to communicate science to society is an interesting added value of VECTOR. Close collaboration between
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon Europe proposal on 'Becoming National against the State: Popular discontent and adherence to minority nationalisms in late nineteenth-century Eastern Europe'.

assistant: **Innovation**

Based on transnational comparisons, our project probes into non-elite adherence to minority nationalisms in rural Eastern Europe between the 1870s and the First World War. We compare six case studies. We focus on state agency as a driving factor, to offer a corrective to the currently predominant, one-sided emphasis on the nexus between nationalist activists and the people. In the broader Eastern Europe, most national movements defined their constituencies in disregard of existing borders. We try to demonstrate that the states structures in which these populations lived inadvertently conspired with national movements to constitute minorities disaffected with them. They did so on two levels. First, by expanding their infrastructural reach, they inflicted harm especially on rural people and made themselves an easier target for economic and social grievances. Second, they reinterpreted certain populations as foreign, a problem and a threat to the security and integrity of the state. They implemented special policies to assimilate or contain them and proactively framed their dissatisfaction as a sign of nationalist yearnings. By retrieving voices from below, we propose to clarify how far these processes aided successful national mobilization in minority. Some of our more specific research questions are the following. What hopes did rural people attach to national movements? Were they interested in modern public services from a state controlled by their coethnics – or rather, did they expect national movements to shield them from any kind of state interference? What state policies did they react against, and how did these differ from the ones that the elites of national movements resented? Did popular voices about the state administration and policies echo elite framings? Fi