RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Solar and Heliospheric Collisionless Kinetics: Enabling Data Analysis of the Sun to Earth Plasma System with Kinetic Modelling' (SHOCK), addressing dissemination and exploitation.

assistant: **Impact**

This project brings together leading European groups working in the area of kinetic modelling of space plasma to enhance and accelerate the effective scientific exploitation of existing space plasma data sets and to maximize the scientific return of future space missions, both European and international. The fundamental concept of the project is to take advantage in the European context of the synergies between space plasma modelling and data analysis. At the present time there is a vast store of collected data from robotic space plasma missions and also an increasing awareness that kinetic processes at small length scales and short time scales are crucial for a proper understanding of the fundamental processes which govern the dynamics of heliospheric plasmas from the solar corona outwards. The top level aims of this project are: (1) To provide a focus for the increased use of kinetic simulations in enhancing space data analysis for European Space Agency (ESA) missions such as Cluster, Cassini, Ulysses, Helios, BepiColombo, and Solar Orbiter, but also for international missions such as Themis, MMS, Solar Probe Plus, etc.; (2) To take forward the state of the art in research using kinetic plasma simulations of space plasma systems, so that new models are developed and exploited allowing the synergies between data analysis and code validation to emerge; and (3) To improve our knowledge and understanding of the Sun to Earth plasma system, and fundamental processes such as turbulence, shocks, particle acceleration and reconnection, which have relevance for other astrophysical environments.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Seventh Framework proposal on 'Microbial genes in motion: phage and the nature of lateral gene transfer in freshwater lakes'.

assistant: **Innovation**

Freshwater quality was defined as a key environmental theme by the European Environment Agency's State and Outlook 2010 Report.  A large threat to freshwater quality is the accelerated eutrophocation of lake waters due to high human impacts from agriculture and industrial and residential wastes, leading to eutrophocation and in some cases toxic cyanobacterial blooms. Microbial communities in eutrophic lakes contribute to a positive feedback loop which perpetuates eutrophocation. The persistence of and capability for dissemination of specific genes related to microbial metabolism in lake environments is unknown.  Phage transduction is the primary mechanism by which gene transfer events occur in bacteria.  Phage have the possibility to mediate LGT between very distantly related bacteria and transfer virulence factors and genes for metabolic adaptation across taxonomic boundaries and over long phylogenetic ranges. In this study, we will characterise microbial and phage populations as well as phage-mediated LGT in Finnish lakes using culture-independent techniques and high-throughput sequencing to determine: 1) the native microbial and phage populations in lakes at different trophic levels, 2) the cohort of functional genes carried by phage, and 3) how broad the host range of resident phage is.  We hypothesize that phage provide a reservoir for genes allowing niche adaptation and increased fitness in response to nutrient stress and/or deprivation. Additionally, we will be able to identify whether phage contribute to the persistence and spread of cyanobacterial toxin genes, and/or providing a reservoir for genes which contribute to the positive feedback loop of eutrophication.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Back to basics: reactivating fundamental processes that repair the developing brain to rebuild neuronal circuits following injury' under Sixth Framework.

assistant: **Concept**

The objectives of the proposal are- to improve recovery from brain injury by increasing our understanding of how to reproduce in the mature brain the natural repair that follows injury to the immature nervous system; and- to expand the applicant's battery of techniques for investigating this important international problem.We will achieve this with a multidisciplinary approach to a simple in vivo model with well-defined structure, function and repair during development, to identify mechanisms underlying repair at molecular, cellular and circuit levels.The model is the rat climbing fibre input to the cerebellum. If this path is destroyed unilaterally early in development, new connections arising from surviving neurons provide circuit repair at anatomic, synaptic and behaviour levels.Exogenous growth-factors partly recreate this plasticity in the mature cerebellum. This project will investigate the mechanisms by which, one growth factor (BDNF) recreates developmental plasticity.The investigation will involve:- surgical removal of climbing fibres from a hemicerebellum and BDNF treatment to induce plasticity.- gene array and in situ hybridisation on tissue collected during reinnervation to identify candidate genes whose expression is altered in reinnervating and target cells.- single cell RT-PCR to compare gene expression associated with reinnervation (from b) in reinnervated vs. non-reinnervated targets, since not all available targets are reinnervated.- examination of the role of each gene in reinnervation by increasing/decreasing its function by viral vector transfection of cerebellar neurons to over-express either the active molecule or relevant blocking protein.The relevance to the Specific and Work Programs is that the exchange of ideas and expertise through research
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Bioinformatic approaches to identify and detect both disease- and drug-related genomic alterations in breast cancer patients' (BRIDGES).

assistant: **Concept**

Breast cancer is the most common cancer among European women showing high clinical and molecular heterogeneity. Current clinical management causes patients overtreatment with implications on both patients’ quality of life and healthcare costs. Moreover, intrinsic or acquired tumor resistance to treatment leads to incurable metastatic progression in a significant proportion of patients. Advances in cancer genomics highlighted a high inter- and intra-tumor genetic heterogeneity, reinforcing the need for a mutation-based personalized treatment and a way to non-invasively monitor evolving disease. This project will significantly contribute in addressing such unmet challenge aiming 1) to identify altered breast cancer driver pathways, 2) to study their association with drug response and 3) to develop tools for a non-invasive assessment of such alterations. By integrating multi-dimensional molecular data from more than 3000 cases, driver pathways will be identified and their association with previous breast cancer classifications as well as their prognostic significance will be studied. Their predictive power will be investigated in a matchless bio-bank of Patient Derived Xenografts, a much more reliable pre-clinical model, able to recapitulate inter- and intra-tumor heterogeneity observed in patients. Multi-dimensional molecular data and high throughput drug screenings are available and will be integrated to identify novel pharmacogenomics associations.Mining of such amount of data will allow defining a portfolio of relevant breast cancer alterations that will be sought in plasma of patients from the DETECT trial, towards a non-invasive monitoring able to guide therapeutic strategy.Development and application of cutting-edge computational approaches is fundamental to reach a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'High-Density Peptide MicroArrays and high-throughput, label-free detection of peptides, modifications and interactions' (HIPAD).

assistant: **Innovation**

Proteins are extremely malleable building blocks of life involved in all aspects of biology. Many diseases are caused by proteins aberrations, and proteins are frequent targets of intervention. Mapping all proteins and their functions are expected to yield pervasive medical and biotechnological benefits. However, even the most comprehensive and high-throughput proteins discovery technologies are seriously challenged by the extreme diversity and low abundance of many proteome components; a problem, compounded by the lack of affinity reagents and validated probes for sample preparation and identification. Our concept is that shorter protein fragments, or peptides, may offer solutions to many of these problems as peptides may represent or mimic proteins. Using in situ solid-phase peptide synthesis, computerized photolithography and novel photochemistry, we have recently generated peptide microarrays of up to 2 million addressable peptides. This unprecedented high-density and high-content peptide microarray technology could make inroads into the kind of high-throughput analysis needed to address the entire human proteome. Here, we aim to exploit this potential by using and improving three different, yet complementary, label-free detection technologies allowing sensitive, high-resolution determinations of the identity, quality and/or modification of individual members of a peptide microarray, and real-time monitoring of any interacting molecular receptor. We will also develop peptides as rapid, specific, and renewable affinity reagents for complex sample preparation, and develop peptides as probes and complex biosensors. Three SME’s constitute the backbone of this collaboration, receiving 50% of the budget, and enjoying significant opportunities from the booming protein/pept
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Development of globin expression vectors for human gene therapy' (1994-1998) under Fourth Framework.

assistant: **Objectives**

The long term correction of sickle cell anaemia and thalassaemia by gene therapy requires the stable integration of a fully functional a- or beta-globin gene into the genome of a sufficient number of bone marrow (stem) cells of the patient. To achieve this two major problems will have to be addressed, namely stable delivery and expression. At present the efficient introduction and stable integration of genes into a host cell genome can be achieved with known retroviral and adeno associated viral (AAV) vectors. The most efficient elements to express a gene in a stable manner are LCR's. The objective of this proposal is to construct a LCR containing beta-globin transcription unit and viral vector technology that could lead to gene therapy clinical trials. As an attractive alternative we will also start the development of a non viral gene transfer system based on ligand mediated gene transfer. This system has the potential advantage that it has less size constraints and that it can be produced on large scale.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Roles and mechanisms of helper NLR phosphorylation in plant immunity' (2021-2027).

assistant: **Concept**

Plant diseases caused by microbes result in considerable yield losses in agricultural production systems worldwide, thereby endangering food security. To combat invading microbes, plants have evolved a two-layered innate immune detection system: cell-surface receptor-initiated pattern-triggered immunity (PTI) and intracellular nucleotide-binding leucine-rich repeat receptor (NLR)-initiated effector-triggered immunity (ETI). Interestingly, cell-surface receptors are required for full NLR-mediated plant immunity. NLRs are classified as sensor NLRs, involved in effector detection, or helper NLRs, required for sensor NLR signalling. Our preliminary data demonstrate that in Arabidopsis thaliana the helper NLR N REQUIREMENT GENE 1 (NRG1) undergoes PTI-dependent phosphorylation, which plays an important role in NRG1-mediated programmed cell death. This suggests that helper NLR phosphorylation may be a crucial checkpoint for PTI/ETI mutual potentiation. This project aims to unravel the molecular mechanisms by which NRG1 is phosphorylated and how its phosphorylation potentiates NLR-initiated immune signalling. My host institute, the Sainsbury Laboratory (TSL), has all the resources to carry out this research. During my work supported by this fellowship, I will be trained in molecular biology, biochemistry, genetics, proteomics and bioinformatics, which will lay the foundation for my future career as an independent researcher. In turn, I will bring my specific expertise in phosphorylation-based activation of cell-surface receptor complexes in Nicotiana benthamiana and tomato, which is not currently available in my host lab. The findings of this project will advance the understanding of plant-microbe interactions and should provide new strategies for crop protection and plant bree
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'End-to-End Efficiency (E3)', explaining its relevance to EU policy priorities under Seventh Framework.

assistant: **Impact**

The End-to-End Efficiency (E3) project is an ambitious FP7 EC Integrated Project (IP) aiming at integrating cognitive wireless systems in the Beyond 3G (B3G) world, evolving current heterogeneous wireless system infrastructures into an integrated, scalable and efficiently managed B3G cognitive system framework. The key objective of the E3 project is to design, develop, prototype and showcase solutions to guarantee interoperability, flexibility and scalability between existing legacy and future wireless systems, manage the overall system complexity, and ensure convergence across access technologies, business domains, regulatory domains and geographical regions.<br/><br/>Cognitive radio systems are seen by many actors of the wireless industry as a core technical evolution towards exploitation of the full potential of B3G systems. It is under way to revolutionize wireless communications just as the PC revolution. E3 will ensure seamless access to applications and services and exploitation of the full diversity of corresponding heterogeneous systems, in order to offer an extensive set of operational choices to the users, application and service providers, operators, manufacturers and regulators. E3 will optimise the use of the radio resources and spectrum, following cognitive network paradigms. The management functions will be distributed over different network elements at various levels of the system topology.<br/><br/>The E3 consortium will bring together major key European players in the domain of cognitive radios and networks, self-management and end-to-end reconfigurability. E3 will build on several key achievements from the successful FP6 E2R Programme, pursuing research into the most promising directions, in order to facilitate the vision of true end-to-end connectiv
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Dynamics of Adaptation and Resistance in Cancer: MApping and conTrolling Transcriptional and Epigenetic Recurrence' (2021-2027).

assistant: **Concept**

Tumours evolve, transforming from early-stage curable disease into treatment-refractory, deadly cancer. Therapy resistance is arguably the biggest problem in oncology today, and much of it remains unexplained. The central hypothesis of this proposal is that a large proportion of unexplained drug resistance is due to heritable epigenetic alterations, and non-heritable transcriptional plasticity in cancer cells. I refer to these mechanisms as the dark matter of cancer evolution. Genetic, epigenetic and transcriptional adaptation, together with changes in the tumour microenvironment, may happen at the same time in the same tumour. Lack of knowledge of these mechanisms hinders the development of new treatments strategies. Tackling drug resistance requires a unique combination of clinical cohorts, experimental models, evolutionary biology and computational methods.I will map and quantify the mechanisms and evolutionary dynamics of genetic and non-genetic drug resistance at unprecedented scale. I will focus on colorectal cancer, the third most common cancer and second leading cause of cancer-related death worldwide. I will use patient-derived organoid models, matched to clinical cohorts followed longitudinally. I will measure organoid evolution under the pressure of cancer drugs, with and without the tumour microenvironment. I will track cell lineages with lentiviral barcoding and perform longitudinal single cell multi-omics, measuring genomes, epigenomes and transcriptomes of the same cell. I will interpret the results within a unique computational framework that brings together evolutionary theory with machine learning to measure, predict and control resistance.This project will identify new mechanisms and dynamics of cancer drug resistance, deliver new predictive models, a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Low power short range wireless communications' (2002-2006).

assistant: **Innovation**

The objective of the program is to enhance an existing 24 person wireless products development team at Duolog by adding two senior researchers and four researchers. The researchers will bring a high level of competence in low power wireless system design at system, architecture and sub-block level. This expertise will be applied to development of an 802.15.4 system-on-chip with driver software, and to the Multiple Input Multiple Output blocks required for wireless LAN 802.11n intellectual property products.This will allow Duolog to develop low power wireless LAN designs for home network multimedia systems and wireless PAN systems for wireless sensor networks. The researchers will benefit by working with an experienced wireless implementation team and developing their research to be applied with all the constraints of a real-world product. ISLI (Institute of System Level Integration) will also provide ultra-low power system level design knowledge transfer and training for two man months to an existing staff member.Wireless platforms including 802.11 (WLAN) and 802.15.4 (WPAN) form an important part of Europe's application technology roadmap for microelectronics as described by Media+. The research will take place in Galway, which is in the Irish Border, Midlands and West region (identified as a less favoured region of Europe). Researchers will be recruited from active research groups in Europe or elsewhere.