RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Establishment, modulation and inheritance of sexual lineage specific DNA methylation in plants' (SexMeth) under Horizon 2020.

assistant: **Objectives**

DNA methylation is an epigenetic mechanism carrying regulatory information across generations in plants and animals. Germlines – called sexual lineages (SLs) in plants – are essential for understanding methylation-based epigenetics because they mediate inheritance and undergo large-scale methylation reprogramming. Germline methylation reprogramming is also crucial for reproduction. However, our understanding of plant SL epigenetics is in its infancy. I have done some of the first and most influential work in plant SL epigenetics, and developed advanced techniques for the isolation and epigenomic analysis of rare plant cell types. Recently my lab discovered that the small RNA-directed DNA methylation pathway, which generally only targets transposons, induces methylation of genes specifically in SLs, thereby regulating gene expression and meiosis. Our results also indicate that genic methylation is established in meiocytes (the origin of the male SL) by soma-derived small RNAs that are attenuated by heat stress, suggesting the hypothesis that environmentally malleable heritable information flows from soma to the germline. We will test our hypothesis and reveal the molecular mechanisms underlying SL-specific DNA methylation in plants by pursuing the following objectives: 1. Determine how SL-specific genic methylation is established in meiocytes2. Reveal how sRNA biogenesis and transport mediate DNA methylation in the male SL3. Elucidate environmental modulation and transgenerational inheritance of SL methylationOur research will reveal how new genomic loci become methylated and stay methylated through cell divisions, and how methylation is adjusted by the environment and carried to the next generation to influence phenotype. This knowledge will revolutionize our understand
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Triggering Mechanisms of Snow Slab Avalanches' under Seventh Framework.

assistant: **Concept**

Snow avalanche is the most frequent type of slope failure in mountainous areas of the world. In total, approximately 15% of Europe’s and 6% of Earth’s land may be considered as avalanche prone-areas. Avalanches claim hundreds of lives annually worldwide and there have been historical cases with death tolls between hundreds and thousands of people during one event or winter season.One of the main goals of snow avalanche research is to forecast natural or induced mechanical failure of a sloping layered snowpack in order to estimate potential damage to human activities in mountain regions and to be able to conduct risk-oriented planning. The deadliest type of snow avalanches are slab avalanches, which start from a failure of a cohesive horizon of snow, or “slab”, underlaid by a so called “weak layer”. The nature of weak layer deformation is considered the crux of slab stability. At present, the origin of initial failure and the failure process in the weak layer remain poorly understood.In order to contribute to development of a mechanical model of snow fracture, and to provide tools to estimate initial conditions for avalanche dynamic models for hazard mapping and risk management, we propose to investigate the mechanical behavior of weak layers and their relationship to slope-scale instability.Key objectives of the study are: 1) To design instrumental apparatus for both cold laboratory and in situ field work to study the mechanical behavior of snow weak layers. 2) To obtain, through experiment, fully operational mechanical laws of different types of alpine snow weak layers. 3) To critically review existing modeling approaches for weak layer and slab in order to couple best existing practices with experimentally obtained results. 4) To relate the micro and meso-scale weak l
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Fifth Framework proposal on 'Laser scanning microscopy of interfacial microbial communities'.

assistant: **Innovation**

The position is in the area of interfacial microbial ecology with focus on laser scanning microscopy. The research should concentrate on the structure and function of bio film systems that are crucial for microbial processes in the environment. Major emphasis will be the in site investigation of complex procaryotic and/or eucaryotic microbial communities at interfaces. For this purpose sample preparation, staining techniques, laser-scanning microscopy with 1 -photon and 2-photon excitation as well as 3-dimensional digital image analysis will be employed. The project may involve microcosm experiments at various dimensions. A solid background in basic light and epifluorescence microscopy would be of advantage. The UFZ Department of Inland Water Research Magdeburg was founded in 1992 and moved in 1998 into a new building. The scientific staff includes 40 people with training in various disciplines such as ecology, limnology, biology, microbiology, chemistry, physics and mathematics. The group Microbiology of Interfaces consists of 4 scientists, 3 technicians as well as a variable number of post docs and PhD students. The group has advanced equipment including: confocal laser scanning microscope, 2-photon laser scanning microscope, UNIX workstations for digital image analysis, bio film microcosms, microelectrode equipment, pilot scale technical laboratory. Furthermore, the analytical groups offers state of the art facilities such as: HPLC, GC, GC/MS, LC/MS - AAS, ICP-OES, ICP-MS, TXRF - SFA, IC.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Seventh Framework proposal on 'EPIGENomic markers for AIR pollution-induced health effects'.

assistant: **Concept**

Exposure to particulate matter (PM) has detrimental effects on the health outcomes. Recently is shown that particulate matter increases the risk for lung cancer at the population level, which is assessed within the framework of the European Study of Cohorts for Air Pollution Effects (ESCAPE). This study dramatically increased the epidemiological weight of this association, however mechanistic evidence at this level is mostly scarce. Epigenetics, the heritable changes in gene expression that do not involve a change in DNA sequences such as DNA methylation can modulate genomic functions under exogenous influence such as air pollution. So far, several lines of research suggest that the mechanisms involved are through inflammation and oxidative damage. In spite of the specific DNA methylation results a large genome-wide epigenetic analysis in relation to ambient air pollution has not been performed, as such this gap in knowledge will be addressed by this proposal.In this project I hypothesise that DNA methylation of inflammation and oxidative stress pathways are key in PM-induced effects. I aim to identify changes in methylation related to exposure to specific air pollutants by profiling full genome methylation data. For this project I will combine exposure and epigenetic data from three large prospective studies of over 1200 individuals from the ESCAPE (FP7), HuGeF (Human Genetics Foundation, Italy) and EnvironGenoMarkers (FP7) studies. I will explore the association of genome wide DNA methylation (Illumina 450K) with ambient air pollution in healthy subjects (n=1200) with detailed air pollution measurements, including personal estimates of PM2.5, PM10, PMcoarse, NO2, NOx and traffic indicators.  I will perform a study of pathways and networks that PM exposure affects foll
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'CoeLux: the sky light reconstruction in artificial illumination by means of solid transparent nanocomposites'.

assistant: **Objectives**

The quality of artificial illumination is a major concern in modern society, where living conditions often deprive us of the comfort of natural light. The global replacement of incandescent with fluorescent or white-LED lighting, foreseen owing to energy-saving constrain, will represent impoverishment in terms of colour-rendering capacity, thus raising the problem to the level of health care. In a scenario where all scientific and industrial efforts are spent on developing low-consumption light sources with the same spectrum as that of the sun, “CoeLux” enters with a ground breaking proposal, based on the evidence that the sun cannot provide natural light in the absence of sky. With the aim of producing those colour distributions captured by artists in their masterpieces, “CoeLux” develops nanocomposite materials, incorporates them in lighting installations, and so recreates the same atmospheric light scattering that produces all colour variety in transmitted sunlight, and the blue tinge in the shade under diffused sky light. In so doing, spectacular sky-and-sun illumination in noon or sunset modes, under a clean or stormy-day sky, will be made available for apartments, working areas, malls, sport centres, railway stations, as well as for squares, parks, and stadiums, during night time. The technology is compatible with low-consumption fluorescent lamps or LED’s, being less demanding in terms of source quality. Finally, “CoeLux” will disseminate the results beyond the project by promoting a European exhibition in which such technological apparatus is shown to provide a unique perspective for a deeper understanding of the connection existing between fine art, architecture, literature, photography and our everyday experience of light.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Identifying mechanisms and novel treatments of bone pain – closing the gap to the clinic.' (BonePainIII).

assistant: **Innovation**

Musculoskeletal pain from diseases such as arthritis and cancer in the bone affects the quality of life of millions and is a major societal burden. The current treatment options are opioids, non-steroid anti-inflammatory drugs and disease modifying drugs; however, these are often insufficient in providing an adequate pain relief. This is due to dose limiting side effects, the risk of misuse and addiction (opioids) and lack of efficacy. Thus, there is a strong unmet medical need. Drug development in pain has been hampered by a poor translation of preclinical data to the clinic and a lack of understanding of the underlying mechanisms. Therefore, in this project 4 academic beneficiaries, 3 industrial beneficiaries, 2 industrial partners and 4 academic partners join forces to create an outstanding and integrated training program for early stages researchers to increase the clinical translation of bone pain research and drug discovery and to provide novel mechanistic insight and potential therapeutic targets. We will develop fully humanized organ-on-a chip models of the painful bone disease microenvironment and we will use humanized mouse models, and tissue and liquid biopsies from patients to obtain clinically relevant data. Moreover, we will identify new disease mechanisms and potential treatment targets and explore the efficacy of clinical stage therapeutics in mouse models of the painful skeletal diseases osteoarthritis, rheumatoid arthritis, cancer in the bone and the rare bone disease, fibrous dysplasia. In an extensive training effort covering both specific research skills and transferable skills, the students will obtain an interdisciplinary, state-of-the-art and innovative training from the participants across sectors.  The students will benefit from secondments wit
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon 2020 proposal for the project 'OvAge: an innovative, user-friendly digital methodology that quantifies ovarian reserve by integrating clinical, biochemical and 3D-sonographic data to help decision-making in all phases of woman life'.

assistant: **Objectives**

OvAge s.r.l. is the owner of a pioneering patented medical device-software able in quantifying women ovarian reserve (both in term of fertility potential and distance to menopause) by integrating clinical, biochemical and 3D-ultrasonographic data.For the first time in this field of medicine, this instrument, also called OvAge®, gives as a result explicitly the ovarian age of a woman, a ready-to-use information that is going to revolutionize the relationship of women with themselves and with the Specialists in the field of Gynaecology.OvAge s.r.l. objective is to diffuse its innovative research product all over the world, with the purpose of helping both women (healthy or affected by infertility, benign or malignant pathologies) and physicians (general and specialists) in the management of many frequent conditions affecting the reproductive life of a woman.In this way, OvAge® aims to contribute the sustainability of health care systems, by reducing both costs related to late diagnosis of infertility and those related to useless surgeries in women affected by benign pathologies for which menopause is the best and cheaper therapy. Moreover, OvAge® will solve women needs for life planning by predicting reproductive potential and for safely solving common pathologies by predicting distance to menopause.To reach these goals, a feasibility study is needed. It will produce a market study for each European member state,proposals on best practice to enhance customer loyalty, a risk assessment of the plan and a potential licensing in order to launch OvAge® software medical device in the EU market initially through a web site. Later, OvAge s.r.l. aim to commercialize its product in the rest of the world (phase 2) as a medical hardware device.The most relevant market segment for OvA
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Scalable modelling and analysis techniques to study emergent cell behaviour -- understanding the E. coli stress response' will create measurable scientific, societal and economic impact aligned with Sixth Framework.

assistant: **Impact**

The aim of our research is to enhance our understanding of the molecular mechanisms underlying the behavior of complex living systems. In particular, we investigate how large and complex regulatory networks control the response of a living cell to its ever-changing environment.The stress response to environmental events is induced by the interaction of several interwoven modules with complex dynamic behavior, acting on different time scales. To deal with this complexity we need mathematical modeling and computer-supported analysis. So far, the methods of systems biology are mostly applied to individual modules, such as particular metabolic pathways and genetic networks. But to understand the global behavior, we need an integrated description of the regulatory network. We must develop new, scalable methods for modeling and analysis of integrated networks.We will apply our methods to the stress response system of the enterobacterium Escherichia coli, one of the best-studied organisms and a model for several pathogenic bacteria. In the project, we will develop an integrated model of the stress response system of E. coli, including key metabolic, genetic and signaling modules. We also develop methods to reduce high-dimensional nonlinear systems of equations, and to approximate them by discrete automata. Parallel model checking algorithms will be developed for verifying specific properties at the model. So we can explore the models and predict the outcome of experiments.Finally, we will validate our model by experimentally testing predicted properties in our labs. Our cross-disciplinary European team of biologists, mathematicians and computer scientists will develop a coherent and generic methodology, generalizable to other complex (biological) systems. Our models will be ac
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Molecular design and application of splice-switching antibody-oligonucleotide conjugate for hypertrophic cardiomyopathy-related MYBPC3 splice modulation.' (HCMSpliceAOC) targeting Horizon Europe.

assistant: **Abstract**

Familial hypertrophic cardiomyopathy (HCM) is the most common inherited heart disease and is predominately caused by mutations in genes that encode proteins associated with cardiac sarcomeres, the functional unit critical for basic contractile heart function. Nearly two-thirds of individuals with HCM experience mild to no symptoms, whereas the remaining one-third experience dyspnea, chest pain, or arrythmias that can progress into heart failure, causing sudden cardiac death, particularly in young competitive athletes. Current strategies to treat HCM include invasive procedures such as septal or left ventricular reduction therapies or cardiac implantation. Such methods are not curative, but rather aimed to treat symptoms and not the pathophysiological cause of the disease, thus highlighting the importance of new molecular therapies that can prevent HCM. One potential method to correct HCM mutations is through the use of antisense oligonucleotides (ASOs), short polymeric nucleic acid biomolecules, that can correct aberrant RNA splicing, a fundamental biological process for gene expression, by binding to pre-mRNA splice sites and block recognition by the spliceosome. However, delivery of ASOs to cardiac cell models, including cardiomyocytes differentiated from human induced pluripotent stem cells (iPSC-CMs), remains to be one of the biggest challenges in ASO efficacy. Herein, this project proposes to design and test the effects of chemically-modified, splice-switching antisense oligonucleotides conjugated to an antibody, an antibody-oligonucleotide conjugate (AOC), using a unique traceless linker strategy that has the potential to not only treat splicing defects in HCM, but also provide proof-of-concept of improved cell delivery of ASOs. AOCs will be tested in iPSC-CMs bea
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'High Performance PEM Electrolyzer for Cost-effective Grid Balancing Applications' in the context of a Horizon 2020 call.

assistant: **Objectives**

The next generation water electrolysers must achieve better dynamic behaviour (rapid start-up, fast response, wider load and temperature ranges) to provide superior grid-balancing services and thus address the steep increase of intermittent renewables interfaced to the grid. The HPEM2GAS project will develop a low cost PEM electrolyser optimised for grid management through both stack and balance of plant innovations, culminating in a six month field test of an advanced 180 (nominal)-300 kW (transient) PEM electrolyser. The electrolyser developed will implement an advanced BoP (power tracking electronics, high efficiency AC/DC converters, high temperature ion exchange cartridges, advanced safety integrated system, new control logic) and improved stack design and components (injection moulded components, flow-field free bipolar plates, Aquivion® membranes, core-shell/solid solution electrocatalysts). Several strategies are applied to lower the overall cost, thus enabling widespread utilisation of the technology. These primarily concern a three-fold increase in current density (resulting in the proportional decrease in capital costs) whilst maintaining cutting edge efficiency, a material use minimisation approach in terms of reduced membrane thickness whilst keeping the gas cross-over low, and reducing the precious metal loading. Further, improving the stack lifetime to 10 years and a reduction of the system complexity without compromising safety or operability. All these solutions contribute significantly to reducing the electrolyser CAPEX and OPEX costs.  HPEM2GAS develops key technologies from TRL4 to TRL6, demonstrating them in a 180-300 kW PEM electrolyser system in a power-to-gas field test; delivers a techno-economic analysis and an exploitation plan to bring the in