RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Hyperpolarized sensors for probing metabolism in microfluidic organ-on-chip platforms' (MicroHyperProbe) targeting Horizon Europe.

assistant: **Abstract**

Hyperpolarized (HP) magnetic resonance imaging has emerged as a promising method for probing the biological characteristics of living tissue. A sensor molecule (e.g. pyruvate, fumarate, acetate) is hyperpolarized and injected into a patient, and the resulting metabolic flux can be used to assess cell viability, tumour response to therapy, and perform pH mapping, to name a few examples. Two significant barriers stand in the way of the widespread implementation of HP imaging: (1) the current hyperpolarization method, dissolution dynamic nuclear polarization (dDNP) is cumbersome, expensive, and requires expertise to operate the equipment, and; (2) preclinical method development relies heavily on the use of animal models which inhibits rapid screening of experiments. To overcome these limitations, in this project I will: (1) implement parahydrogen-induced polarization as the hyperpolarization method, which is significantly cheaper, easier to use, and can produce the hyperpolarized metabolites at a higher rate than dDNP, and; (2) perform the hyperpolarized imaging in organ-on-a-chip devices, which closely mimic in vivo conditions but allow for experiments to be performed at a greater rate, and can use human tissue as opposed to animal tissue to more closely mimic real conditions. The specific focus in this project will be to investigate whether HP metabolic imaging can provide insight into the progression of muscular dystrophy in human muscle tissue.


**Keywords:** Microfluidics,  In vitro cell and tissue imaging
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Novel tools for real time monitoring and quantification of protein aggregation in Parkinson s disease and related neurodegenerative disorders' (2007-2013).

assistant: **Innovation**

To understand the molecular basis of any biological process, it is critical that one is not only able to visualize and monitor molecular events that underlie this process, but also to possess the tools to manipulate these events in a spatial and temporal fashion both in and out of the cell. The overall objective of this proposal is to apply chemical biology approaches to allow real time monitoring of protein aggregation and to dissect the role of specific disease-associated post-translational modifications, phosphorylation, nitration, and truncation on the structure, aggregation, and biochemical properties of monomeric a-syn in health and disease. To achieve these goals, we plan to use a combination of organic chemistry, molecular biology, proteomics, protein engineering, and semisynthetic strategies to facilitate site-specific introduction of post-translational modifications that can be masked and activated in a controllable manner, both inside and outside living cells. Modified synthetic ±-syn will be introduced into primary neurons and cellular models of synucleinopathies and the consequences of masking or activating specific modifications will be assessed using biochemical, immunofluorescence, and live imaging techniques (Specific Aim 1). The absence of specific molecular probes that allow in vivo monitoring and quantitative measurement of toxic misfolded and aggregation intermediates represents a major impediment to understanding the relationship among protein misfolding, post-translational modification, protein aggregation, neurodegeneration, and cell death in PD and other neurodegenerative disorders. To address this challenge, we plan to develop and characterize novel antibodies that target different species along the amyloid formation pathway of ±-syn (Specific
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon 2020 proposal for the project 'Structural and cellular investigation of the regulation of ATP8B1/CDC50A,  a human flippase important for the hepatic function'.

assistant: **Objectives**

Lipid flippases mediate transport of phospholipids from the exoplasmic leaflet to the cytosolic one, and their role is to establish and maintain phospholipid asymmetry in eukaryotic membranes. This asymmetry is tightly regulated and it is implicated in numerous cellular processes such as membrane trafficking, signaling, blood coagulation, apoptosis, cytokinesis, or cell fusion. The human genome encodes 14 different P4-ATPases, a subset of them being linked to pathologies such as mental retardation, Alzheimer’s disease, diabetes, cancer or liver disorders. As concerns the latter disease, it has been demonstrated that mutations of the human lipid flippase ATP8B1, are responsible for inherited intrahepatic cholestasis. The intrahepatic cholestasis affects mostly children and is characterized by pruritus, recurrent jaundice, growth failure and an evolutive liver degradation which requires transplantation before adulthood in most severe cases. Up to now, little is known about the function of ATP8B1 at the canalicular membrane and this is mainly due to the lack of consensus regarding its transport substrate and how its activity is regulated. Preliminary results indicate that ATP8B1 has a broader transport substrate specificity than previously reported in the literature and therefore its study will widen our understanding of the biochemical basis of lipid selectivity in lipid flippases. In addition, in the proposed project I aim at understanding how a disease related lipid flippase, ATP8B1, is regulated in vitro and in vivo and at understanding functional and structural consequences of identified mutations of ATP8B1 linked to the intrahepatic cholestasis.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Probing the quantum nature of gravity at all scales' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

To understand the quantum structure of spacetime, we must confront proposed theories of quantum gravity with experimental data. This is a challenge, because quantum-gravity effects are generally assumed to manifest at the Planck length, which lies far beyond current experimental reach. I challenge this assumption by probing quantum gravity at different scales. To achieve this, I propose to use lever arms that translate predictions of quantum gravity at the Planck scale into predictions at larger distances, where we can compare them to experimental data. I have identified such lever arms in particle physics, black holes and cosmology. The proposals first testing ground for quantum gravity is particle physics, where the scale dependence of couplings is a lever arm that translates Planck-scale predictions into testable predictions at larger distances. Using this lever arm, I will predict the values of couplings in the Standard Model and models of dark matter. The second testing ground is black holes, where the spin can act as a lever arm that translates Planckian physics into horizon-scale effects. To use this lever arm, I will derive spinning black-hole spacetimes and explore their image features. The third testing ground is late-time cosmology, where the dark energy is a lever arm that translates a microscopic interplay of scalar fields with gravity into an accelerated expansion of the universe. To use this lever arm, I will predict the properties of dark energy.The proposals main objective  to confront quantum gravity theory with observational data  will thus be achieved by forging new connections between quantum gravity and other research fields. These novel connections also provide a fresh perspective on long-standing questions in these other fields, for example on th
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon 2020 proposal on 'arTificial neuRal nEtwork prediction of hAemorrhagic Transformation in STROKE.'.

assistant: **Concept**

Short door-to-needle times are critical in acute ischemic stroke, as stroke progresses over time and hence treatment benefits are time dependent as well. It is estimated that about 1.9 million brain cells are lost per minute in untreated stroke cases, which emphasizes the time urgency of stroke care. Early identification of salvageable tissue is crucial in management and treatment of patients suffering from acute ischemic stroke to avoid further tissue injury – and current approaches are time consuming and highly dependent on clinician’s skills. We at Combat Stroke need an innovation associate (IA) – Biomedical Data Scientist – to aid us in developing a novel fully automated personalized clinical decision support system (CDSS) for optimal treatment of acute ischemic stroke patients – the HTSafe solution under development at CS. Our company is an SME with limited capacity to mobilize the job market and attract highly skilled professionals to continue the development of the HTSafe. Particularly, the demand for data scientists is far outstripping supply worldwide. Job trends data confirms the rise in popularity for the position, showing that the number of job postings for data scientist positions increased by 1700% between 2012 and 2016. Thus, the H2020-INNOSUP initiative will bridge the gap in our company to develop a novel and disruptive product, which has a large commercial potential to Combat Stroke. Moreover, the programme includes a tailored and core EU training to support the development of new skills and competences of the IA.The international CDSS market is currently worth over €360 million, and it is growing at astonishing 21.5% CAGR to reach over €1.1 billion by 2022. Given the high incidence of IS cases and the high importance of quickly allocating the appropri
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Lifecycle-reconfigurable Smart Mobility Platform to enable autonomous and cost-effective personalized solutions for social inclusion of disabled and elderly while leveraging AM technologies' (FreeWheel).

assistant: **Innovation**

FreeWheel promotes social inclusion of disabled and elderly people through a urban mobility solution consisting of a unit integrating an autonomous “smart active” module, multiple custom interfaces and an app. Such a solution can be either rented (a sharing service available at in-door and out-door urban facilities), or sold; in the latter case the unit includes a fully personalized wheelchair. FreeWheel satisfies the need for customization, both on the user and on the vehicle side, through the implementation of a modular concept based on standard reconfigurable, low-cost modules (e.g. engine, gears, control unit, HMI, etc.) and on ultra-customized interfaces (e.g. body-to-vehicle, engine-to-vehicle, vehicle-to-infrastructure, etc.), produced via additive manufacturing. Re-configurability is achieved through an easy exchange of standard modules in different products. A further objective is making affordable the lifecycle cost of the above-mentioned mobility service (and/or product) through an innovative business model that offers mobility as a service (independent of vehicle ownership) and by leveraging dematerialization (reduction of manufacturing lead times and investment costs) and product re-configurability. The expected impact is:-Social inclusion of elderly and disabled citizens: the project will demonstrate affordability and safety in accessing public spaces in urban areas;-Short time-to-market: 70% less than the conventional approach. -Significant cost reduction in the manufacturing of a personalised wheelchair unit as a result of the re-usability and adaptability of personalised products components; -Ultra-low environmental impact: re-usability of modules and efficient manufacturing leading to more than 70% in environmental impact reduction regarding primary re
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Autonomously Healable Thermoplastic Polymer Coatings based on Cooperative Interactions' (PolyHeal).

assistant: **Concept**

Modern society demands for materials that are reliable, for safety reasons, durable, to offer prolonged service lifetimes, and cost- and energy-efficient, to preserve natural resources and produce minimum waste and environmental impact. In this context, self-healing polymers are “smart” materials with the ability to repair themselves autonomously or to heal on-demand upon exposure to an external stimulus. However, the broad commercialization and universal use of self-healing thermoplastic polymers is still hampered by a main problem that resides in the balance between self-healing ability and mechanical properties at working conditions. Along the ERC-StG project PROGRAM-NANO, we found a quite promising solution by introducing cooperative effects in supramolecular polymers. In this way, thermoplastic materials were obtained that combined toughness and resistance with self-healing ability at ambient temperature. This PoC Project now focus on evaluating the viability of our approach for producing commercial self-healing thermoplastic coatings by addressing 3 strongly interconnected milestones: 1) Test and Validate the technical characteristics of the materials and the self-healing process; 2) Design and implement a market analysis and study the best IPR strategy for this new technology; and 3) Optimize an efficient methodology toward a primary commercial product with optimum performance and minimum cost. These tasks require the assembly of a team comprising experienced researchers on one hand, to validate PolyHeal performance through well-established standard tests, and management, market and IPR experts on the other, to find the suitable market niches and develop the most convenient strategy for the exploitation of our technology. Due to its innovative nature and versatil
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Centre for scientific visualisation' will create measurable scientific, societal and economic impact aligned with Sixth Framework.

assistant: **Impact**

The main objective of this project is to strengthen and increase existing e-infrastructure research capabilities of the Centre of Information and Computer Science of Rudjer Boskovic Institute towards an internationally recognised, state-of-the-art research and education centre for scientific visualisation technology contributing at all levels of the scientific efficiency and higher education.The foreseen benefits of the project are immediate increase in the competency level in research and development capability of the Centre; provision of the state-of-the-art research experience to some postgraduate students (equipment and environment, access to leading scientists in Europe); strengthening of the collaboration capability on research projects with regional (WBCs) and EU based institutions; ability to exchange researchers between the Centre and leading European Institutions; provide transfer of up-to-date technology into regional industries, and increased participation in EU-FP projects. Thus, establishing the Centres of Excellence Network in the WB region that shall be orientated to distribute parallel computing and scientific visualisation on the computer grid environment, is our important aim.The objectives of the project will be achieved by the set of SS A activities in the areas of networking, improvement of human resources, exchange of personnel, visiting researchers, and equipment acquisition, all in the framework of the Centre's continuous development and integration with European leading international institutions and Centres of Excellence. By these specific support activities, the e Science technology will be introduced to the national and regional research community. The contribution of e Science technologies is acknowledged on the Grid platforms in the direct
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'DNA-Based Molecular Nanowires' goes beyond current state-of-the-art under Fifth Framework.

assistant: **Innovation**

Current microelectronics is rigorously based on silicon. DNA-based electronics is a prime example of an entirely alternative approach that, we believe, will enable to reduce the size of the current devices by ~1000 times. A specific innovative focus of this project is to investigate novel DNA-based derivatives that hold potential for improved properties with respect to native DNA. The derivatives that we suggest, G4-DNA, M-DNA and PC-DNA, pioneer as modified bio molecules that are proposed as electrical molecular wires. The research proposed here will provide a unique combination of state of the art capabilities for the production of DNA-based molecules, unique surface chemistry, scanning probe microscopy and spectroscopy and electrical transport measurements, sophisticated nano-fabrication, and leading theoretical skills, all combined to provide new solutions and information about conduction through DNA derivatives.OBJECTIVESThe general objective of our collaborative research is to develop DNA-based nano-electronics. For that purpose we will:1. Produce G4-DNA, M-DNA and PC-DNA molecular nano-wires and characterise their electrical properties;2. Develop a control of the molecule-electrode and molecule-substrate interaction;3. Develop a theoretical understanding of the energetic and the conduction mechanisms in these wires;4. Develop model nano-electronic devices on the basis of the above DNA-Based wires.DESCRIPTION OF WORKThe G4-DNA and M-DNA, with and without a variety of redox active metal ions, will be produced by the biochemistry group of Kotlyar. This group will continue to produce the wires in an increasing maximum length. It will modify the chemical structure of the molecules according to the feedback obtained by the 'measuring' groups and the theoretical modelli
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon 2020 proposal on 'Advancing a Precision Medicine Paradigm in metastatic Colorectal Cancer: Systems  based patient stratification solutions'.

assistant: **Concept**

Colorectal cancer (CRC) is the third most common cancer in Europe with c. 420,000 cases and 150,000 related deaths in 2012. Of total CRC cases, it is estimated that approximately 50-55 % harbour RAS mutations. Current treatment for RAS mutant (mt) metastatic(m) CRC is primarily based on 5-fluoruracil based chemotherapy +/- bevacizumab. However, there are currently limited treatment options once cancers have become resistant. Moreover, while therapy optimization strategies in RAS wild-type CRC patients are feasible, targeted treatment of microsatellite stable (MSS) RAS mt disease is difficult and has not evolved significantly in recent years. COLOSSUS will deliver novel concepts for disease-mechanism based patient stratification in MSS RAS mt mCRC to address the need for stratified or personalised therapeutic interventions in this setting. The consortium will integrate multidimensional and longitudinal omics data to identify new MSS RAS mt  specific subtypes with unique signalling dependences. We will harness the power of systems biomedicine, network analysis and computational modelling  to identify new actionable pathways, biomarkers and targets across subtypes. These targets will be interrogated in state of the art pre-clinical patient derived xenograft studies. Newly described MSS RAS mt classifiers will be validated as novel patient stratification tools within the COLOSSUS trial. SME partners will develop clinically relevant and commercially viable assays for outcome prediction and stratification of MSS RAS mt patients based on novel classifiers. The impact of assays on CRC associated healthcare costs will further be assessed. Patient associations will be included and the proposal will consider regulatory aspects and commercialisation opportunities, in particular for