RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'STING signalling modulation via the Electron Transport Chain' in the context of a Horizon 2020 call.

assistant: **Objectives**

The innate immune response to cytosolic dsDNA via the cGAS-STING pathway has been shown to be essential in the response to various viral, bacterial and parasitic infections, in autoimmunity and in cancer. However, the relationship between STING signalling and metabolism is currently unknown. In recent years, our understanding of immunometabolism has also increased – it is becoming clear that metabolic adaptations are fundamental for immune responses. Due to its role in this wide range of clinical pathologies, the mechanism of STING signalling has received a great deal of scientific interest and several STING-targeting compounds are now reaching clinical trial stages of development. It is therefore important to understand the effect of STING activation on mammalian cells, as well as how to manipulate this response to the greatest effect. The research proposed here focuses on the relationship between cellular metabolism and innate immune signalling via STING, using an unbiased proteomics approach as well as targeted immunometabolism approaches to find STING modulating metabolic pathways.Having completed my PhD studies in immunology, I am pursuing a postdoctoral project, in the lab of Dr. David Sancho at the CNIC, that utilises my expertise and allows me to expand my technical horizons and enhance my career development. As an expert in innate immune signalling I believe that combining my knowledge with the Sancho lab’s expertise in cellular immunobiology and metabolism will lead to a better understanding of our immune system. As well as the expertise of my host lab, I will also benefit from the collaboration of leaders in proteomics and mitochondrial biology. This Individual Fellowship would greatly benefit my current research objectives and my career as a whole by allowin
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Automotive Tested High Voltage and Embedded Non-Volatile Integrated System on Chip platformemploying 3D Integration', explaining its relevance to EU policy priorities under Seventh Framework.

assistant: **Impact**

Automotive represents 12% of the EU industrial GDP. 20% of the value of a car is already electronics and 10% of this are IC components. Minimizing costs and space for additional functionality requires further integration. The EU project ATHENIS has successfully addressed System-on-Chip (SoC) integration of CMOS, high voltage and embedded memory for harshest automotive conditions. Further cost reduction will require even higher levels of integration. Therefore ATHENIS_3D will provide the industry's first 3D heterogeneous integration technology platform for harshest automotive conditions with Through Silicon Vias (TSV) and Wafer Level Packaging (WLP). A demonstrator car will prove the functionality of the 3D integrated electronics for an electrical machine with start/stop function and the industry's first 3D/TSV/WLP DCDC converter with integrated inductor for the new 48V standard. Cost savings from integration and a 5x reduction of PCB area at improved reliability will be shown. For this purpose substantial technological barriers such as flipchip mounting of a 90nm CMOS FPGA on a 180nm HVCMOS Si interposer with Integrated Passive Devices (IPD), high density MRAM and magnetic sensors all meeting reliability requirements up to 200C application temperatures have to be mastered for the first time. This will be achieved by combining TSV and HV-CMOS technology from ams with CMOS and Cu-TSV technology from CEA-Leti, MRAM technology from Crocus and WLP technology from Besi.  Platform scalability will be proven by flipchip packaging of 14nm CMOS samples on the interposer. New modules for TSVs, MRAM and Passives embedded in TSV technology will be developed to enable 200C applications. Valeo will provide system specifications, development and demo car evaluation. The other partners
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'RNA Polymerase III Rpc4/Rpc5 subcomplex and Selenocysteine tRNA transcription' (2014-2020), addressing dissemination and exploitation.

assistant: **Impact**

In higher eukaryotes, the RNA polymerase III (Pol III) participates in the transcription of small RNAs such as the tRNAs. RNA polymerase recruitment to their specific promoter relies on the activity of several transcription factors. Brf2 is a transcription factor that exclusively recruits RNA Pol III at the selenocysteine tRNA (tRNASec). Unpublished work from our group has unravelled an unanticipated central role of Brf2 in the oxidative stress response pathway, by acting as a cellular blockade during prolonged oxidative stress.We are interested in understanding the molecular determinants that govern RNA Pol III recruitment at tRNASec promoter and its interaction with Brf2-bound promoters. In general, RNA Pol III subunit’s size is conserved amongst the eukaryotic kingdom. However, an exception is the human Rpc5 subunit, whose C terminus has 450 residues that are not present in its yeast counterpart C37. Similarly to Brf2, the Rpc5 C-terminal extension is only present in higher metazoans, which suggests a phylogenetic link between these two proteins. The recruiting mechanism of RNA Pol III to Brf2-dependent promoters has not been described to date. Preliminary results in our lab provide evidences that indeed Rpc5 C terminus is responsible for the accurate recruitment of RNA Pol III at TBP/Brf2/DNA complex. Interestingly, structural homology predictions indicated that the human Rpc5 C-terminal extension is a eukaryotic homologue of the prokaryotic protein SelB, a factor that interacts with the tRNASec and with a specific region of mRNAs, the SECIS-element, during translation of SeCys containing proteins. This similarity suggests a regulatory role for Rpc5 C terminus in the interaction with the SECIS-element and/or the tRNASec.Our main objectives are to determine the struc
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'DENIsovan anCESTORs in Sahul: deciphering human evolution through molecular techniques' (DENI-CESTOR) under Horizon Europe.

assistant: **Objectives**

Field and science-based discoveries over the last decade have revolutionised our understanding of human evolution outside of Africa. We now know that at least 6 or 7 hominin groups lived in Eurasia in the past 200,000 years. Some of these, i.e. Denisovans, Neanderthals and early modern humans, met and interbred with genes from extinct archaic hominins passing down to modern humans living today. Recent DNA studies have shown that selected populations in Southeast Asia, and Oceania in particular, have inherited genetic material from Denisovans, with the highest percentage found in Papua New Guinea (PNG) and Aboriginal Australians. Multiple divergent ancestries are seen in PNG with two unique Denisovan lineages; the latest (D1) occurring as late as ~ 30-15 k years ago. While hugely relevant for understanding hominin dispersals, admixture and adaptation, the Pleistocene and early Holocene archaeological and palaeoanthropological records of PNG are poorly understood. The rarity of hominin fossils from this region is a significant limitation in testing current hypotheses on Denisovan presence in Sahul during the Late Pleistocene. The aim of this project is to analyse two previously excavated archaeological assemblages from highland PNG (Yuku and Kiowa, with the oldest dates ~10-15 ka BP) to expand the record of hominin fossils and provide detailed information on the taxonomy and chronology of these unique assemblages. Through extensive application of Zooarchaeology by Mass Spectrometry (ZooMS) analyses of unidentified bone fragments, radiocarbon dating, stable isotope analysis, and ancient DNA analyses I will analyse new hominin fossils as well as understand early subsistence strategies and adaptation to new environments. This will be focused on the Papuan Highlands which is
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Fifth Framework proposal on 'Safe and high cycle recharging electromechanical battery'.

assistant: **Innovation**

Having a very high rate of recharging (>5x1000000), the electromechanical battery is considered to be most promising energy storage system for mobile and stationary applications therefore, the project proposed in the 2nd phase FLYSAFE" is focused on safety and certification issues of high speed flywheel systems made of modern composite materials. Extensive material, rim and component testing also at elevated temperatures will supply reliable information concerning fatigue, durability and certification of advanced flywheel systems. Based on detailed analytical and experimental investigations of possible failure modes of composite flywheels, guidelines for the containment design will be established, additionally. The implementation of sophisticated systems measuring changes within the system, e.g. vibrations, is considered to be a feasible approach to improving reliability of supporting components. In this way the economical production and certification of a safe and high cycle recharging flywheel will be possible, so that the competitiveness of the EXPLORATORY Award "FLYSAFE-LIFE" partner CCM and EAST-4D is strengthened significantly."
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Structural integrity for lifetime management – non-RPV components' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

The overall objective of the project is to assess, optimise and develop the use of advanced tools for the structural integrity assessment of reactor coolant pressure boundary components (RCPB) relevant to ageing and life time management and to integrate the knowledge created in the project into mainstream nuclear industry assessment codes. Realistic failure models for some of the key components will be identified within the project based on the operators’ experience supported by an efficient knowledge management database system. The range of assessment tools considered will include those for assessment of component failure by advanced fracture mechanics analyses validated on small and large scale experiments, quantification of weld residual stresses by numerical analysis and by measurements, stress corrosion crack initiation/growth effects and assessment of RCPB components (excluding the reactor pressure vessel) under dynamic and seismic loading. Based on theoretical and experimental results, performance assessment and further development of simplified engineering assessment methods (EAM) will be carried out considering both deterministic and probabilistic approaches. Integrity assessment case studies and large scale demonstration experiments will be performed on Mock-ups of safety-relevant components. These will include a repair weld in an aged butt-welded austenitic pipe, a dissimilar narrow gap TIG weld (following the EPR design) and a cladded ferritic pipe. Moreover experiments on specimens and feature test pieces will be carried out to support the large scale Mock-up analyses. The end product of the project (“STYLE TOOLS”) will comprise best practice guidelines on the use of advanced tools, on improvement and qualification of EAM as a part of European Leak-before-b
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon Europe proposal on 'Addressing Critical Gaps in mpox epidemiology in DRC - The MOVIE and TRACE studies'.

assistant: **Innovation**

This proposal outlines two linked studies aimed at addressing the public health challenge of Mpox in the Democratic Republic of Congo (DRC). The MOVIE study focuses on understanding the kinetics of viral elimination shedding light on how MPXV interacts with host tissues and immune defences, which is crucial for developing containment strategies and potentially influencing endpoint selection in therapeutic trials. The TRACE study aims to determine secondary attack rates in MPXV outbreaks, assessing host susceptibility within specific populations, offering vital data to target interventions towards vulnerable groups and informing vaccine efforts by contributing to the assessment of vaccine efficacy endpoints.


**Keywords:** mpox, viral kinetics, secondary attack rate
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Mechanisms of deaminase-independent inhibition of HIV infection by APOBEC3 proteins' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

The human immunodeficiency virus (HIV) continues to be a major public health issue. Current treatments can slow down disease progression, but high costs, severe side effects and the emergence of drug-resistant strains all call for the development of new treatment regimes. The detailed understanding of virus-host interactions is of utmost importance for this endeavor. The cytidine deaminase APOBEC3 (A3) proteins, and in particular APOBEC3G (A3G), are potent host restriction factors that can be packaged into assembling HIV-1 virions and severely compromise viral infectivity. To evade this powerful restriction, HIV-1 relies on the vif (viral infectivity factor) protein, which has evolved to promote proteasome-dependent A3G degradation. Despite intensive research and the high potential of the vif-A3G axis for therapeutic intervention, the precise mechanisms of A3G packaging and HIV inhibition are not fully understood. As a polynucleotide editing enzyme, A3G induces hypermutation in the viral genomic DNA. Additionally, it has recently become evident that A3G also interferes with the process of reverse transcription in a deaminase-independent way. The research proposed here is designed to elucidate the molecular basis of A3G-mediated inhibition of cDNA synthesis during reverse transcription using a combination of experimental approaches and systems. This involves: 1) The assessment of reverse transcription efficiency in the presence and absence of A3 proteins in vitro as well as in physiologically relevant human CD4+ T cells. Importantly, this will include a novel assay designed to map 3’ termini of nascent viral cDNA products. 2) Mapping of A3G binding sites on viral genomic RNA and identification cellular RNA binding partners using CLIP (crosslinking and immunoprecipitation
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'New Graphene Imager based on Graphene-on-wafer' (G-IMAGER) under Horizon 2020.

assistant: **Objectives**

G-IMAGER’s goal is to create added value through the introduction of high performing Graphene-on-wafer at competitive cost, accelerating innovation for the advanced electronic industry and eventually creating a new product category. This new area of development will make it possible for the European industry to harness this high market potential, fostering competitiveness and creating growth throughout the EU. The consortium proposes this project after an internal Business Innovation Plan, in order to take the CVD Graphene-on-wafer technology a step closer to the market through a new Graphene Imager product. This project will allow GS and EMB to become the worldwide leader as Graphene related electronics producers with the necessary production capacity to supply the industry and the research laboratories. More specifically, the G-IMAGER objectives are: • To develop ready-to-integrate Graphene-on-wafers for the advanced electronic industry.• To set up a customized rapid prototyping line and a new “foundry service”.• To produce a new Graphene Imager product and validate it for industrial acceptance.• Increase process yield and implement an automated and optimised quality control considering market requirements• Scale production capacity to more than 10,000 wafers/year to demonstrate commercial feasibility and business growth GS has validated and patented a highly efficient Graphene-on-wafer production process and its application into electronic devices. The consortium will be able to introduce Graphene Imager products at an industrial scale positively impacting the SWIR camera market of $ 1,100 M. The consortium will become the worldwide leader in Graphene-based advanced electronics and generate a cumulative net income of at least €60 M in four years and +33 jobs.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Nucleolar Responses to DNA Damage: rDNA, an emerging hub of genome instability' (NUCDDR) targeting Horizon 2020.

assistant: **Abstract**

DNA lesions can impose serious threats to genome integrity and cell viability. Whereas DNA damage may occur anywhere in the genome, it is increasingly recognized that certain genomic loci rich in repetitive sequences display increased susceptibility to damage and are linked to chromosomal rearrangements and malignancy. Clusters of ribosomal DNA gene (rDNA) repeats, present on five different chromosomes, constitute the most heavily transcribed area of the human genome and are organized in a nuclear membrane-less organelle, the nucleolus. So far, putative links between rDNA damage and malignant processes have not been rigorously assessed.We will address the hypothesis that rDNA repeats represent a major hub of genomic instability contributing to malignant transformation. Using state-of-the-art experimental systems that allow enrichment for nucleolar DNA damage, we will explore: (i) hypothesis-driven and mass spectrometry-based approaches to define regulators of the rDNA damage response; (ii) live imaging and advanced molecular biology tools to uncover how histone epigenetic changes and formation of RNA:DNA hybrids impact on nucleolar chromatin, nucleolar organization, rDNA transcription and repair ; (iii) cell models that recapitulate malignant transformation caused by inducible oncogene expression or epigenetic inactivation of tumour suppressors, to assess replication stress in rDNA repeats as a primary source of genomic instability and pertinent to hallmarks of cancer.The proposed research is expected to yield novel insights into the signaling networks and biological processes regulating rDNA damage and repair within the nuclear environment and define how these mechanisms are corrupted during neoplastic transformation. This knowledge could be directly applicable to the
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Fifth Framework proposal on 'Latin america thematic network on bioenergy'.

assistant: **Innovation**

Good management of resources, alleviating poverty and improving the socio-economic conditions of living are key objectives for research and development efforts in Developing Countries and the EU partnership with Developing Countries. In order to promote the sustainable use of baroness in Latin America it is the general objective of this project to establish a network of Knowledge Centres (Universities and R&D Institutes) and SMEs from Latin America and the European Union. For this purpose a number of partners with excellent expertise in the field of biomass are selected and an effective co-operation between the partners is assured. The main focus of the project will thereby be the development of policy options for the promotion of decentralised biomass energy production.