RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Novel therapeutic antibodies for multiple myeloma' (MMSC).

assistant: **Innovation**

Multiple Myeloma (MM) is an incurable plasma cell neoplasm with a median survival of 3 years.  A critical step towards the development of more effective therapies is the identification of dominantly acting oncogenes. We have implemented an integrated oncogenomic approach that utilizes high-resolution gene-specific array-CGH and comprehensive expression profiling to identify the spectrum of genetic alterations in a large panel of MM cell lines (n=46) and annotated primary tumors (n=65).  These efforts have uncovered a strikingly high number of novel recurrent focal amplifications and deletions. To select the most critical amplicons, a stringent filtering algorithm that takes in account the amplification level, recurrence and the presence of similar lesions in an extensive array-CGH database of more than 600 tumors and cell lines from different cancer types yielded 13 highly-selected amplicons. Within these, 117 genes demonstrated significant upregulation when compared with normal plasma cells, with 28 genes considered potential targets for Monoclonal Antibodies based on their structural features. This dataset will be prioritized by key parameters including oncogenic validation assays and in vivo testing.  The ultimate goal of this proposal is to provide a list of functionally validated antibody targets, which can be enlisted into therapeutic monoclonal antibody development.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Seventh Framework proposal on 'Study of coherent non-linear optical response of nanoparticles and application to multiphoton imaging in cell biology'.

assistant: **Concept**

The objective of this research project is to develop a novel multiphoton microscopy technique and to investigate its application to selected problems in cell biology which require sensitive three-dimensional imaging, in-vivo and real time. This novel technique is based on the detection of the resonant coherent non-linear optical response (four-wave mixing) of colloidal quantum dots (CQDs) to explore their application as bio-labels beyond their fluorescence properties. This method would retain many of the advantages of multiphoton fluorescence microscopy, such as intrinsic sectioning capability, and would offer additional advantages such as coherent detection free from fluorescence backgrounds. We expect the spatial resolution to be increased by a factor of two due to the optical non-linearity, resulting in a 130nm of lateral resolution at 550nm exciting wavelength and objectives with 1.3 NA. To compare advantages and disadvantages of this novel coherent optical microscopy technique with respect to confocal/multiphoton fluorescence microscopy, we will investigate a model system, namely HeLa cells, which provides well established routes for biolabelling using both dye-labelled antibodies, protein fusions with fluorescent tags and bioconjugated colloidal quantum dots. The application of this microscope has the potential to bring a significant step forward in the fundamental understanding of major areas in cell biology, which can not be fully addressed with the conventional fluorescence microscopy tools. Moreover, the dependence of fundamental electronic properties, such as homogeneous linewidth of excitonic transition and exciton-phonon interaction, on the size and composition of quantum dots will be investigated via the measurements of the transient four-wave mixing signa
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Absorbed natural gas system whith guard bed device (ANGUARD)' (ANGUARD) under Fifth Framework.

assistant: **Objectives**

Objectives and Problems to be solved: Small scale or bulk storage of natural gas is problematic: most storage methods have high associated costs as well as operational restrictions on size, location, safety criteria and delivery rates. There is a clear need for a more efficient, lower cost and safer means of storage of natural gas, both for small scale portable applications in large scale district storage. The ANGUARD consortium intends to address this challenge by developing an innovative form of storage based on the use of adsorbed natural gas (ANG) in which natural gas is physically adsorbed on activated carbon. The key objective of this project is to develop a guard bed system to reversibly filter out trace components of natural gas during filling of the storage vessel. This will then be integrated by design with the main storage vessel for small and large-scale systems, thus enabling this technology across the entire range of storage capacities. Description of the work: A carefully planned and managed set of Work Packages has been devised to take the work to a successful conclusion. These include· Definition of guard bed requirements for both stationary and vehicle applications, · Identification and production of optimised guard bed adsorbent material, · Engineering laboratory testing of guard bed systems under simulated ANG adsorption cycles, · Development of a detailed model of adsorption kinetics, · Design, build and testing of a prototype vehicle guard bed assembly, · Scale-up design development· ANG safety assessment study These complementary elements will jointly produce a sound design capability for ANG storage and guard bed systems for application to both stationary storage and vehicle applications. Additionally, the project will provide a basis for pre-nor
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'The first robust and cost-effective floating vertical axis wind turbine for deep water offshore' under Horizon 2020.

assistant: **Concept**

Offshore wind is stronger, steadier and more frequent than onshore. Existing solutions in this industry are bottom-fixed and floating wind turbines, both based on Horizontal Axis Wind Turbines technology and marked by certain limitations. Bottom-fixed turbines can only be installed in waters of up to 50m depth, while floating ones struggle to produce energy at a competitive cost, mainly due to very high CAPEX/OPEX.SeaTwirl opens up this vast market potential with a disruptive offshore turbine that combines the Vertical Axis Wind Turbine technology with an effective and smaller spar-buoy. SeaTwirl has key advantages over competition:  -Economic: it’s cheaper; has -14% CAPEX, -22% OPEX & -46% DECEX, less service & maintenance; -20% LCoE.-Technical: few moving parts; works regardless of wind direction; can be placed closer together; provides power continuously; uses the water’s buoyancy to carry the weight.-Environment: clean energy; low noise levels; safe for birds; helps protect marine environment.SeaTwirl is highly scalable too. First, we will develop S2, a 1-MW turbine which will pave the way for larger ones: 7MW (2025), 10MW (2028) & >30MW thereafter.SeaTwirl will cover the needs of offshore users without connection to a power grid: a) Isolated public municipalities (Islands/Coastal areas), b) Offshore industry companies (fish farms/oil & gas rigs). We will target these 2 buyer groups, who will acquire S2 for their own use, as well as energy distribution companies that will provide with energy those who do not wish to invest in S2.This project is key for our further development and our huge potential: the offshore wind industry is one of the world’s fastest growing sectors, estimated to grow at a CAGR of 7.7% by 2017-2025.By 2025, we expect to 1) sell 118 MW installed
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon 2020 proposal on 'Identification and Characterization of Cis-Regulatory Module Dysfunction in Rett Syndrome with ROQ-WACh'.

assistant: **Innovation**

Precise regulation of gene expression is achieved through the coordinated action of genomic cis-regulatory modules (CRMs). The identification of CRMs has long been a goal of functional genomics as CRM dysregulation can have devastating consequences for health and development such as autism. For example, mutations in the MeCP2 gene, which encodes protein that binds methylated DNA and regulates gene expression in neurons, results an Autism Spectrum Disorder termed Rett Syndrome. While thought to modulate CRM activity as a repressor, MeCP2’s function remains ambiguous and would benefit from a functional genomics characterization. To identify CRMs I recently developed a novel approach called FIREWACh. Here, I propose to utilize FIREWACh first to identify active CRMs within a homogenous population of neurons, identifying genomic loci of CRMs whose function may be compromised by MeCP2 mutations. I will adapt FIREWACh to allow the quantification of CRM output in a new method I propose to call ROQ-WACh (regulatory output quantification within accessible chromatin) by addition of barcodes to reporter mRNAs. This will allow high-throughput readout of CRM activity globally in vivo and will be broadly applicable to many biological fields. Lastly, I aim to combine the above approaches to quantify the changes in CRM output in response to pathological mutations in MeCP2. This Marie Sklodowska-Curie Action will allow me (the Experienced Researcher, Matthew Murtha) mobility to Spain to join the laboratory of Dr. Manel Esteller (Host Supervisor), renowned epigeneticist and expert in DNA-methylation biology to perform the research. Together the approaches and data generated by this MSCA action will provide key insights into the relationship between methylated DNA, precise control of gene
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon Europe proposal on 'A Neonatal anaerobic Gut-microbiome-on-a-Chip  to decode bacterial colonization  and infant gut T cell maturation'.

assistant: **Concept**

Developing a healthy host-microbiome symbiosis during the first 1000 days of human life is critical for lifelong health. Despite its importance, our understanding is limited and derives from animal studies or associative human trials. Therefore, there is a pressing need for an infant gut model to capture dynamic microbiome-epithelium-immune interactions in early life. In NeoGutChip I will develop and validate a first-of-its-kind in vitro human Infant Gut-Microbiome-Immune-on-a-chip model (iGuMI) that will faithfully simulate the complex anaerobic environment of the infant gut, which current models cannot achieve. I will capitalize on my cutting-edge GuMI and microbiome engineering experience, to ensure that iGuMI is microbiota- and immune-competent. I will use iGuMI to determine the impact of dietary and microbial factors (especially early bacterial colonizers) on the development and maturation of infant gut microbiota, the mucosal barrier, and immune cells, and unravel the keystone bacterial species and key genes driving these effects. I will realize these objectives through autologous coculture of infant colonic organoids derived epithelium and naïve immune cells in iGuMI. Using bacterial colonization and long-term coculture in iGuMI, I will identify a set of infant gut species driving the immune maturation, and key bacterial genes through RNA sequencing, metabolomics, and loss-of-function assays. Finally, I will investigate mechanism-guided intervention of the microbiota-immune axis in iGuMI to promote anti-inflammatory T cells and mucosal barrier function in infant gut. The successful completion of NeoGutChip will decode the strain-by-strain view of immune modulation by infant gut microbiota in early life and offer a molecular basis to rationally “design” new interv
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Quantum Devices based on Carbon Nanotubes' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

The aim of this project is to take advantage of the unique properties of carbon nanotubes (CNTs) for the fabrication of innovative nanoelectronic devices. The first nanodevice is a ultra-sensitive detector, designed to probe the electrical properties of individual molecules that are exposed to external perturbations, e.g. such as electric field or light. The detection scheme is based on an original approach, on the contrary to previous experiments which aimed at contacting individual organic molecules with two electrodes. With the two-electrode technique the problems have quickly appeared due to the poor control of the electrode/molecule interfaces. Here, the molecule is attached to only one electrode, a nanotube. The resistance of the nanotube is measured as a function of a gate voltage, which should be sensitive on the energy spectrum of the molecule. Low-current detection is expected to be particularly suitable for molecular electronics, since most of the molecular systems are highly resistive. This includes organic molecules, biological molecules, and semiconducting particles. Most importantly, such an approach is expected to be mostly independent on the quality of the molecule-nanotube interface, and in addition, it allows the device to be operational in higher temperature. The second proposed design of nanodevice is a non-volatile memory, which will be achieved also in one-electrode approach by combining a single nanoparticle (with diameters up to 10nm) with a carbon nanotube transistor. In the device, CNT acts as conduction channel and the charge stored in the nanoparticle behaves as a floating-gate. Charging effects will be obtained either from an atomic force microscope or directly from the nanotube. The objective will be to determine the operability of the dev
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Communication problems in parallel and distributed interconnection networks' to be submitted under Fourth Framework.

assistant: **Abstract**

Communication between processors is a fundamental problem in parallel and distributed architectures, since it is often the major bottleneck lowering the performances of the systems. In this setting the planned research focuses mainly on deadlock-free routing schemes, compact routing and efficient broadcast and gossiping operations. In every case we want to determine optimal and efficient results for the most commonly used interconnection networks both in terms of time required to perform communications and space (memory) necessary at each processor to store the required information. The planned research activity is relevant to industry since the interval routing scheme (one of the routing schemes which will be further investigated) has been recently implemented in the INMOS Transputer Router chips. Moreover the host institute currently maintains contacts with many computer manufacturer companies, such as DEC and SIMULOG. The applicant comes from a less favored region of the Community (Abruzzi - Italy).
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Decoding the complexity of quantitative natural variation in Arabidopsis thaliana' (DECODE) under Seventh Framework.

assistant: **Objectives**

Following a long history of quantitative genetics in crop plants, it now becomes feasible to use naturally-occuring variation contained in Arabidopsis thaliana accessions (lines isolated from natural populations) as the source of quantitative genomics approaches, designed to map QTLs and resolve them at the gene level. Apart from being able to exploit in multiple genetic backgrounds allelic variation that cannot be easily generated by conventional mutagenesis, the (relatively few) success of the QTL studies has often been because of the use of quantitative phenotyping, as opposed to the qualitative gauges used in typical mutant screens. Among the various genetic mechanisms responsible for natural variation that have just started to be revealed, cis-acting regulation is potentially of large impact, despite remaining more difficult to recognize and confirm. The objective of this project is to apply genome-wide quantitative molecular genetics to both, a very integrative and classical quantitative trait (growth in interaction with the environment) and a molecular trait a priori more directly linked to the source of variation (gene expression under cis-regulation). We propose to use a combination of our unique high-troughput phenotyping robot, fine-mapping, complementation approaches and association genetics to pinpoint a significant number of QTLs and eQTLs to the gene level and identify causative polymorphisms and the molecular variation controlling natural diversity. Working at an unprecedented scale should finally allow to resolve enough quantitative loci and pay a significant contribution to drawing a general picture as to how and where in the pathways adaptation is shaping natural variation and improve our understanding of the transcriptional cis-regulatory code.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'EUROpean network of trans-national collaborative RTD in the field of NANOMEDicine' under Seventh Framework.

assistant: **Concept**

Nanomedicine is an emerging field with a large potential for developing public welfare and economic growth. Critical issues for bringing up this potential in Europe concern especially the maturity of the economic players and their capability to move effectively innovation from knowledge to industrial technology and to clinical and public health applications. Industrial players need therefore to collaborate closer with scientific partners and with clinicians. The second issue is the ability of companies to establish multidisciplinary networks with the research and the clinician communities, increasing their efforts towards biological preclinical and clinical validations. This will shorten the delay for patients to benefit from the innovation and increase the competitiveness of European actors. Therefore EuroNanoMed targets the creation of a mechanism to support trans-national collaborative RTD projects between academic laboratories, companies, especially SME’s, and clinicians/public health setting, in the field of Nanomedicine, using a bottom up approach. In consequence, EuroNaNoMed will develop coordinated European-wide programmes based on common joint transnational calls and funding. The thematic of these two calls will focus on the strategic research priorities of the European technology platform “Nanomedicine”: diagnostics, targeted delivery and regenerative medicine. EuroNanoMed achieves an effective critical mass: the 19 participating national/regional programmes expect to dedicate significant budget to the calls, approximately 15-20% of the sum of their national/regional Nanomedicine budgets. The Long Term Vision of EuroNanoMed is the design of a European-wide integrated programme with a coordinated funding. Potential scenario for implementation will be suggested.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Cell polarization in Drosophila' to be submitted under Seventh Framework.

assistant: **Abstract**

Cell polarity is fundamental to many aspects of cell and developmental biology and it is implicated in differentiation, proliferation and morphogenesis in both unicellular and multi-cellular organisms. We study the mechanisms that regulate cell polarity during both asymmetric cell division and epithelial cell polarization in Drosophila. To understand these fundamental processes, we are currently using two complementary approaches. Firstly, we are coupling genetic tools to state of the art time-lapse microscopy to genetically dissect the mechanisms of cortical cell polarization and mitotic spindle orientation. Secondly, we are introducing two innovative inter-disciplinary methodologies into the fields of cell and developmental biology: 1) single molecule imaging during asymmetric cell division, to unravel the mechanism of polarized protein distribution within the cell; 2) multi-scale tensor analysis of epithelial tissues to describe and understand how epithelial tissues grow, acquire and maintain their shape and organization during development. Using both conventional and innovative methodologies, our goals over the next four years are to better understand how molecules and protein complexes move and are activated at different locations within the cell and how cell polarization impacts on cell identities and on epithelial tissue growth and morphogenesis. Since the mechanisms underlying cell polarization are conserved throughout evolution, the proposed experiments will improve our understanding of these processes not only in Drosophila, but in all animals.