RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon 2020 proposal for the project 'Develop and validate appropriate and acceptable outcome measures in intermediate age-related macular degeneration for future interventional clinical trials - Sofia ref.: 116076'.

assistant: **Objectives**

Despite significant advances in the treatment and understanding of late stage age-related macular degeneration (AMD), it continues to be the main cause of irreversible severe visual loss in Europe and its prevalence and incidence will increase with current demographic trends. In order to reduce the significant burden of late stage AMD, novel interventions should aim at stopping or delaying progression from the preceding disease stage intermediate AMD (iAMD) to late stage AMD. As a prerequisite, validated clinical endpoints for iAMD are needed. These should be acceptable to regulatory agencies, health technology assessment (HTA) bodies, and payers. Currently such endpoints do not exist for iAMD clinical trials (CTs).In addition, there is good evidence indicating that patients with iAMD experience some impairment of visual function yet it is unknown to what extend this impacts the patients’ life nor can it be reliably measured and quantified. It is also unknown whether there are specific risk factors in the population of iAMD patients which identify those with more rapid progression to late stages of the disease.Therefore, to enable successful development of iAMD interventions validated functional, morphological and patient--reportedendpoints for CTs, which are clinically meaningful and accepted by regulatory agencies, are required. In addition, functional decline in iAMD, as well as, specific risk factors for iAMD progression to late stage AMD need to be better characterized to inform and improve conduct of future iAMD CTs.Against this background, the major objective of MACUSTAR is to develop novel clinical endpoints for CTs with a regulatory and patient access intention in patients with iAMD. Additional objectives are to characterize visual impairment in iAMD and its pr
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Dispersion force masked-based helium atom lithography' (FabouLACE).

assistant: **Concept**

As Margrethe Vestager, European Commission Executive Vice-President, said There is no digital without chips. Leading-edge semiconductors provide the foundation of the digitalization of entire sectors and industries that are transforming the EU economy and of emerging technologies such as artificial intelligence and quantum computing. Photolithography is currently used for the initial patterning step of chip manufacturing and leading-edge chips require smaller and smaller features. The smallest features that can be produced with this technology (26 nm at present and expected to reach 16 nm in 2028) not only limits the performance and power efficiency of leading-edge chips, but also the opening of new markets linked to quantum devices and silicon devices with integrated light sources. FabouLACE project catches this opportunity through a disruptive lithography approach that uses metastable atoms and dispersion force-based masks and enables features of 2 nm. The technology, currently at proof of concept stage and protected by two patents, will be brought at TRL 6 by the end of the project by developing a prototype. Its performances will be validated together with Imec (key actor in the development, testing and early adoption of nano- and digital technology innovations, including lithography) and end-users in the markets of interest. The spin-off Lace, founded in 2022, will bring the technology to the market by 2031. The technology has already attracted a great interest among investors (450k raised in few months) and among the major foundry Intel that accepted Lace in its highly competitive start-up program intel-ignite. Market and business readiness will be developed together with end-users and leveraging on the economic support from investors (>3M private funding expected
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Covalent chiral functionalization of graphite for enantioselective applications' demonstrating excellence, impact and implementation quality for a Horizon Europe call.

assistant: **Abstract**

An object, or a molecule is said to be chiral if it can be distinguished from its mirror image. Chirality plays an important role in many areas of chemistry, biology, and physics. Many chemical processes involving solid surfaces such as catalysis, crystallization, adsorption, and so forth, are sensitive to chirality. Thus, surfaces are not fertile playground studying chirality, but they can also be used to promote the separation of mirror-image molecules, i.e., enantiomers, due to additional constraints created by the two-dimensional (2D) environment. Because of the reduced symmetry at the interface, these constraints enhance the expression of molecular chirality, allowing not only chiral molecules, but also prochiral molecules to produce chiral superstructures. Even so, recognition processes based on non-covalent interactions could be used to separate enantiomorphs in 2D systems. This new method could be used to resolve racemic mixtures. In this project, nanoconfinement conditions created using scanning probe nanolithography will reveal how the shape and size of the confinement, as well as the nanolithography process itself, affect the chirality of on-surface crystallization processes. Robust chiral surfaces will be created using covalent functionalization of graphite and applied for enantioselective separation. Also, bulk chiral graphitic materials will be synthesized and used as stationary phase in chiral chromatography. SURF-CHIR will allow me to pursue a highly innovative research line in surface science, providing me a perfect platform for my personal development in terms of research and training in a topic of key importance, i.e., chirality, and state-to-the-art imaging techniques. This project will also allow me to strengthen my team-working and leadership skill
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Seventh Framework proposal on 'Investigating the involvement of lincRNAs in aging of haematopoietic stem cells and in haematological malignancies'.

assistant: **Concept**

Changes in chromatin structure are observed during aging and are often a critical step during tumorigenesis. The class of long intergenic non-coding RNAs (lincRNA), has emerged as an important regulator of chromatin structure during normal differentiation, and recently has been implicated in the disregulation of chromatin structure observed in several solid tumours. These lincRNAs could represent an important new class of oncogene. We propose to investigate a potential involvement of lincRNAs in chromatin changes that occur during normal aging of haematopoietic stem cells (HSC), and in acute myeloid leukaemia (AML), an age-related haematological malignancy that is frequently associated with chromatin abnormalities. High-throughput sequencing will be used to compare expression of lincRNAs between young and old HSC, and between AML and normal CD34+ cells. Differentially expressed lincRNAs will be functionally investigated by lentiviral-mediated knockdown and overexpression in cell lines and primary cells, and concomitant changes in chromatin structure will be profiled by chromatin immunoprecipitation. It is expected that this project will clarify the general importance of lincRNAs both in normal aging and in an age-related cancer, and could identify novel molecules which are important drivers of malignancy as well as the functional changes associated with HSC aging. As well as being potential prognostic markers and direct targets for therapeutics in AML, these molecules might also be targeted to improve the function of aging HSCs.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Fifth Framework proposal on 'Effects of nutrient release from Mediterranean fish farms on benthic vegetation in coastal ecosystems'.

assistant: **Innovation**

The objective of this project is to examine effects of nutrients released during fish farming on community dynamics of the benthic vegetation in coastal zones in the Mediterranean.We wish to obtain new and valuable insight within the following subjects:- Fate of nutrients released from aquaculture production in the Mediterranean- Effects of aquaculture on sea-grasses and associated benthic fauna- Shifts in coastal vegetation communities (from seagrass to macroalgae)- Seagrasses and fauna as early warning indicators of aquaculture impactsIn general, more than 70% of the total nitrogen and phosphorus added with the food to fish farms is lost to the aquatic environment (6) and is thereby a source for enhanced pelagic primary production.Very few studies have been able to measure enhanced nutrient concentrations in surroundings of fish farms, suggesting that the nutrients are rapidly incorporated into different components of marine vegetation or exported to the ocean.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Pharmacological and gene therapy targeting of NMNAT2 for neuroprotection in glaucoma', explaining its relevance to EU policy priorities under Horizon Europe.

assistant: **Impact**

The goal of this ERC Advanced Grant is to develop clinically translatable neuroprotective strategies for glaucoma by focusing on NAD metabolism. NMNAT2 is a neuron-specific NAD-generating enzyme and is the upstream enzyme that coordinates axon degeneration. The Williams lab is expert in taking basic biological findings in glaucoma to the clinic, and we will leverage our expertise in this space to develop novel clinically translatable therapeutic interventions for glaucoma based on NMNAT2 biology. In Program 1 my team will further our on-going drug development program developing novel compounds targeting NMNAT2 by:(1)Continuing refining medicinal chemistry to develop an oral and injectable formulation,(2)Confirming NMNAT2 engagement via autoradiography and assessing BBB permeability and distribution via PET radiopharmacology using fluorine-18 radio ligands of these compounds, (3)Assessing the neuroprotective potential of these compounds in proof-of-concept animal models of glaucoma.In Program 2 my team will target neuroprotection and axon regeneration to develop a comprehensive, titratable, single dose treatment for glaucoma via gene therapy by:(1)Combining a regenerative gene therapy (phosphomimetic Protrudin) with neuroprotective treatments (nicotinamide therapy, novel NMNAT2 compounds, NMNAT2 gene therapy),(2)Exploiting the E. coli DHFR and human FKBP destabilising domains to develop a gene therapy product which is rapid, titratable, and switchable (i.e. ON/OFF) and test this in the context of glaucoma.This ERC Advanced Grant will capitalize on my recent findings that targeting NAD can prevent degeneration in animal models and restore vision glaucoma patients and develop crucial in vivo data transitioning this program from TRL 3 to TRL 4. By the end of this funding, w
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Seventh Framework proposal on 'Mouse Model of CoQ deficiency: pathogenesis and treatment'.

assistant: **Innovation**

Coenzyme Q10 (CoQ10) deficiency is an autosomal recesive disorder where the encephalomyopathy and nephropathy seems to be the most important clinical presentations. Some patients respond to CoQ10 supplementation but others do not. In my Postdoctoral stay in Columbia University I identified the first mutation in PDSS2, which encoded the protein responsible for the syntesis of decaprenyl chain. Moreover, I have studied the pathological consequences associated to CoQ10 deficiency in culture fibroblasts with mutations in PDSS2, COQ2, ADCK3 and COQ9. The results show that mitochondrial bioenergetic, the increase of oxidative stress and the defect in de novo pathway for pyrimidines can be the main pathological mechanisms associated to CoQ10 deficiency. However, the tissue specificity need to be investigated since CoQ10 is present in all cells types and is critical for mitochondrial function. In addition, potential treatment need to be tested. Then, I propose: 1) Generate COQ9 knockin mice. Dra. Rahman’s Group has recently found a mutation in this gene in a patient with CoQ10 deficiency. This gene encode Coq9, an enzyme involved in ubiquinone biosynthesis. However, the function of this enzyme is still unkown and I will study it; 2) Charaterize COQ9 knockin mice including behevioural, clinical, hitological-morphological, physiological, bioquemical and genetical studies; 3) Develop and apply different therapeutical strategies: a) Early an late onset treatment with CoQ10; and b) antioxidant strategy (melatonin administration). The results will be essential to undertand and treat the pathegenesis of CoQ10 deficiency, as well as other primary mitochondrial disorders. Likewise, the results can be important in the treatment of neurodegenerative diseases or aging, since CoQ10 suppleme
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon 2020 proposal on 'Secure and Resilient Cloud Architecture'.

assistant: **Concept**

Cloud infrastructures, despite all their advantages and importance to the competitiveness of modern economies, raise fundamental questions related to the privacy, integrity, and security of offsite data storage and processing tasks. These questions are currently not answered satisfactorily by existing technologies. Furthermore, recent developments in the wake of the expansive and sometimes unauthorized government access to private and sensitive data raise major privacy and security concerns about data located in the cloud, especially when data is physically located, processed, or must transit outside the legal jurisdiction of its rightful owner. This is exacerbated by providers of cloud services that frequently move and process data without notice in ways that are detrimental to the users and their privacy.SafeCloud will re-architect cloud infrastructures to ensure that data transmission, storage, and processing can be (1) partitioned in multiple administrative domains that are unlikely to collude, so that sensitive data can be protected by design; (2) entangled with inter-dependencies that make it impossible for any of the domains to tamper with its integrity. These two principles (partitioning and entanglement) are thus applied holistically across the entire data management stack, from communication to storage and processing.Users will control the choice of non-colluding domains for partitioning and the tradeoffs between entanglement and performance, and thus will have full control over what happens to their data. This will make users less reluctant to manage their personal data online due to privacy concerns and will generate positive business cases for privacy-sensitive online applications such as the distributed cloud infrastructure and medical record storage platf
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon Europe proposal on 'Decipher the role of late lytic genes activator, a key determinant of active lysogeny in Listeria monocytogenes'.

assistant: **Concept**

Listeria monocytogenes (Lm) is an intracellular bacterial pathogen. Lm strain 10403S harbors an active prophage in its genome integrated within the comK gene. It was previously discovered by the Herskovits lab (host lab) that during Lm infection of macrophages, the prophage undergoes excision from comK, yielding an intact and functional comK gene that assists the escape of the bacteria from the macrophages’ phagosomes to the cytosol. Notably, this phage excision does not lead to virion production and bacterial lysis in the mammalian environment, suggesting a cooperative phage behaviour. It was further shown that the phage early genes are transcribed in the intracellular niche, whereas the late lytic genes are not, thereby preventing the progression of the lytic pathway. This type of adaptive phage behaviour was termed “active-lysogeny”, representing cases where prophages cooperate with their hosts. To better understand active-lysogeny in Lm, in this BacPro proposal I aim to decipher the mechanism by which the late lytic genes are repressed in the intracellular niche. It was previously demonstrated that the late genes are positively regulated by LlgA, an ArpU-family transcriptional regulator that is encoded by the phage. Preliminary data in the lab indicate that LlgA is thermo-regulated at the protein level. LlgA regulation at the temperature of the mammalian niche was shown to relay on the bacteria, and to involve a mechanism similar to regulated proteolysis. Based on these findings, in my proposal I aim to identify the bacterial proteases that are involved in its cleavage. Further I aim to determine LlgA active form, as well as its cleavage site/s. These studies will provide a molecular insight into the mechanism by which Lm controls the phage in the mammalian environm
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon 2020 proposal on '2D nanomaterials-based composite films for more efficient thermal conduction'.

assistant: **Innovation**

The aim of this proposal is to determine the economic and technical feasibility of using readily scalable technologies for the development of inexpensive and high performance solutions for heat dissipation for the high-end automobile industry, as well as other markets including household appliances, injection moulding, advanced aircraft and pharmaceutical manufacturing, ranging from the actual fabrication protocols, down to a wide range of finite products. The technology here described is focussed to solving heat dissipation issues by the use of novel 2-dimensional (2D) nanomaterials. While graphene is the most well-known 2D system, hundreds of other inorganic layered materials exist. 2-dimensional materials have immediate and far-reaching potential in several high-impact technological applications amongst which are heat harvesting and dissipation.Our technology will offer very cheap, scalable solution of using advanced 2D nanomaterials for enhanced heat transport. Moreover, our technology offers the advantage of being extremely versatile: 2D nanomaterial dispersions can be sprayed on their own directly onto surfaces or they can be mixed to different matrixes such as Polysil to obtain enhanced resistance to wear, abrasion, oxidation etc. This will allow us to improve the performance of existing systems, as well as improve the performance of new designs. Our developed solutions will not need to be applied through the whole heat recovery system, but mainly at those critical parts that limit the system performance. This technology has the potential of becoming a feasible, easy and efficient solution for a range of manufacturing companies. It will constitute a huge economic return, not to consider the societal overall impact of having much more efficient ways to deal with e