RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Single molecule imaging of herpes simplex virus type 1 DNA replication' in the context of a Horizon 2020 call.

assistant: **Objectives**

Herpes simplex virus type 1 (HSV-1) is a widespread and well-known pathogen. However, we still lack a complete understanding of the mechanism of HSV-1 genome replication. We propose to revisit long-standing questions of the HSV-1 genome replication mechanism using cutting-edge single molecule imaging techniques. This approach enables the detection of transient structures and heterogeneous behavior, leading to further crucial insights into this fundamental process. The current model for HSV-1 genome replication comprises an origin-dependent followed by an origin-independent stage. Whereas we do have evidence and a good understanding of the origin-dependent phase, we completely lack evidence for the proposed structures of the origin-independent phase. Thus, in the proposed project we want to study aspects of the origin-dependent replication phase. Specifically, we want to reveal the role of the HSV-1 origin binding protein UL9 during replication as this protein was proposed to act as a switch from the first to the second stage. It has been shown that UL9 is an essential protein during the origin-dependent stage but dispensable and even inhibitory during the second, origin-independent stage. We here propose to use single-molecule imaging techniques to reveal the inhibitory effect of UL9 on HSV-1 mediated replication and to further characterize the specific conditions of UL9 binding to the origins of replication.


**Keywords:** Herpes simplex virus type 1, HSV-1, single molecule imaging, DNA replication
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Fourth Framework proposal on 'LOW COST, IMPROVED SAFETY, ABSORPTION HEAT PUMP'.

assistant: **Innovation**

The aim of the project is the design, construction and testing of a preproduction series including N°17 absorption-type reversible heat-pumps, with two rated power values (11 kW cooling; 25 kW cooling).The design will be focused at :- minimisation of costs- improvement of reliability and safety with respect to competitors- market-oriented overall characteristics.The scope is to gather suitable data and results of testing so as to allow the proposers (SRS) to start with a series production activity and with product commercialisation. The energy technology objective is the dissemination of a technology (absorption combined with heat pumps) which allows a considerable energy saving and a reduction of pollutants emissions with respect to traditional systems of heating and cooling for buildings.The process consists in a thermodynamic cycle which allows to absorb heat from a source at lower temperature and to transfer it to another source at higher temperature. This is achieved through a series of components, prevailingly static. During winter operation (heating mode) the pure ammonia vapour out flowing from the generator is condensed in the condenser. Therefore the liquid ammonia expands in a water cooled evaporator. The ammonia vapour is mixed with a low ammonia solution stream coming form the genrator; the mixture is cooled in the absorber, the condenser and the absorber are cooled by water-glicol mixture of the user circuit. The solution, outflowing from the absorber, is pumped by the diaphragm pump into the generator. The evaporator, during the heating service, is cooled by water of an intermediate circuit; then water is cooled in a finned heat exchanger by the external air.During summer operation (cooling mode), the evaporator is cooled by the water-glicol mixture of th
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Hypoxic regulation of senescent cells secretome in lung cancer and Alzheimer's disease' to be submitted under Horizon 2020.

assistant: **Abstract**

Research over the last decade has convincingly evidenced that cellular senescence and low oxygen (O2) availability (hypoxia) are distinctive features involved in the physiopathology of age-related disorders including lung cancer and Alzheimer’s disease (AD). Cellular senescence is characterised by a permanent and irreversible cell cycle arrest and the acquisition of an intense senescence associated secretory phenotype (SASP). Mounting evidence has shown that the SASP accounts for most of the detrimental effects of senescent cells accumulated in aged tissues. Therefore, understanding the mechanisms that modulate the composition and intensity of the SASP is a fundamental biological question with significant translational implications in age-related disorders. Senescent cells accumulate in tissues where O2 is very limited. To live under these conditions, senescent cells must adapt to hypoxia via the stabilisation of hypoxia inducible transcription factor (HIF). I hypothesise that local hypoxia determines the composition and intensity of the SASP in age-related disorders, a concept that I will name hSASP. Given the intrinsic heterogeneity of the SASP across tissues, I will test this hypothesis in lung cancer and AD models where there is a concomitant accumulation of senescent cells in hypoxic areas. I will use a variety of genotoxic agents to induce cellular senescence in lung cancer and stromal cells as well as in murine primary astrocytes exposed to a decreasing range of O2 tensions. I will elucidate the role of hypoxia in regulating the composition and intensity of the hSASP and its paracrine effects on nearby cells. Finally, I will identify relevant hSASP factors and define their functional effects on lung cancer and microglial cells.Targeting specific hSASP factors may
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Seventh Framework proposal on 'A MEasurement Tool to determine the quality of the Passenger EXperience'.

assistant: **Concept**

Public transport is central to people’s lives and well-being, especially vulnerable groups (e.g. less mobile, elderly and disabled). It is essential in providing access to employment, shops, services and leisure. The need to encourage greater public transport use is critical in achieving sustainability targets. For many, the perception and reality of public transport does not encourage use, especially when multimodal forms of transport are needed. A holistic understanding of passenger experience is critical to develop and support transport accessibility. Whilst previous research has focussed on different aspects of passenger experience, the diversity of tools developed limits their usefulness, effectiveness and transferability. Given the maturity of research, there is a need to synthesize methods and knowledge, to produce a pan-European standardised tool for use across transport modes and with different passenger groups to focus attention away from the design of discrete elements to the whole journey experience. Taking a holistic approach to the study of the passenger experience will provide a bridge between transport, sustainability, design, accessibility and land use; acknowledging the central importance of mobility to quality of life. To plug this gap, METPEX will develop an inclusive passenger experience measurement tool for European transport providers, passenger groups and municipalities validated through its use across 8 sites of varying transport complexity. Its development will be informed by the consortium’s understanding that data collection methods themselves have to be inclusive. The data collected will enable the creation and dissemination of service quality and accessibility benchmark indicators. Through its deployment the tool will have far reaching impa
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Autonomous energy supply system with reversible fuel cell as long-term storage for pv stand-alone systems and uninterruptible power supplies (REVCELL)' goes beyond current state-of-the-art under Fifth Framework.

assistant: **Innovation**

This project develops the reversible fuel cells and brings it into applications for the first time worldwide. The combination of fuel cell and electrolyser in one stack allows a significant cost reduction in the components and the systems. Remote power supply systems for telecom and uninterruptible power suppliers with long back-up times will be demonstrated. All applications have very high requirements on power availability and reliability. R&D on the components and the general system concepts will be perfumed. The consortium consists of technology end-users, component manufactures and research institutions on hydrogen technologies and autonomous power suppliers . The technology opens new markets in rapid growing sectors. The hydrogen storage system replaces batteries and diesel generators which affect the environment. This project fits perfectly with TA K.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Compact Helical Arranged combustoRs with lean LIFTed flames' (CHAiRLIFT), addressing dissemination and exploitation.

assistant: **Impact**

The main objective of the CHAiRLIFT project is to assess an innovative combustor concept capable to achieve an ultra-lean, low NOx, operation of future engines. With this combustion concept the requirements of ACARE Flightpath 2050 will be fully satisfied. The CHAiRLIFT combustor concept comprises two novel features: The first is to adopt “low swirl” lean lifted spray flames which feature a high degree of premixing and consequently significantly reduced NOx emissions. Inherent characteristics of such flames are the strongly reduced risk of flashback and a reduced susceptibility to thermo-acoustics instabilities compared  to conventional swirl stabilized flames. However, such lifted flames bear the risk of lean blow out at some operating conditions.  As second novelty of the CHAiRLIFT concept an alternative approach to standard flame piloting is  proposed, enabling a a further reduction of NOx emissions. Stable and safe operations of the combustor  are ensured  by the interaction of  adjacent flames in circumferential direction within the annular combustion chamber. This requires tilting of the axis of the flames relative to the axis of the machine. This design is called Short Helical Combustor (SHC). It has the advantage that no extra pilot flame is required which may produce additional NOx emissions. Additional benefits are the reduced length of the combustor. Most importantly, the turning angle of the NGV can be reduced resulting into a smaller number of NGV and hence reduced cooling air requirement.Experimental and numerical investigations including the development of an advanced spray atomization model will be carried to assess the NOx reduction capabilities of the concept, by exploiting state of the art methodologies. To explore further NOx reduction capabilities o
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Linking GABAergic neurones to hippocampal-entorhinal system functions' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

GABAergic interneurones can effectively synchronize the activity of principal cells giving rise to distinct oscillatory patterns. A particular rhythm, hippocampal theta oscillations (6-10Hz), links two ways of coding by which pyramidal cells in the hippocampus represent space, namely rate and phase coding. Thus, the theta cycle provides a clock against which the increased firing rate of pyramidal cells in the hippocampus and entorhinal cortex is measured. Furthermore, hippocampal theta is believed to constitute a link to episodic memory. Recent evidence from our lab indicates that recruitment of GABAergic interneurones critically affects certain aspects of hippocampus-dependent spatial memory in mice. We have established genetic tools that allow us to manipulate GABAergic interneurones in a cell type and region-specific manner. In combination with in vivo electrophysiology in the hippocampus/entorhinal cortex and behavioural studies, we will investigate how GABAergic interneurones regulate the activity in neuronal networks and contribute to behaviour. Specifically, we will address the following questions: 1) How does reduced recruitment of GABAergic interneurones affect network activity (theta oscillations)? 2) How does altered activity of GABAergic interneurones affect spatial representation (activity of place cells in the hippocampus and grid cells in the entorhinal cortex)? 3) How does modified activity in the hippocampus affect activity in the entorhinal cortex (and vice versa)? 4) How does modified network activity and spatial representation translate into spatial memory? The interdisciplinary approach will enable us to provide better insight into how cellular activity of GABAergic interneurones relates to network activity and ultimately to behaviour.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon Europe proposal on 'Bayesian Gutenberg-Richter Analysis for understanding Seismic Processes'.

assistant: **Innovation**

Understanding seismic hazard is essential to reducing the risks posed by earthquakes. Our current knowledge comes in part from the study of earthquake nucleation processes and analysing available data on the timing, location and magnitude of past earthquakes. Unfortunately, these seismological observations are limited because we can only detect and use earthquakes above a certain size, making it difficult to estimate critical parameters for probabilistic seismic hazard assessment (PSHA). One of these critical parameters is the b-value, which describes how often earthquakes of different magnitudes occur. Accurate estimation of the spatial and temporal distribution of the b-value is essential for reliable seismic hazard assessment and for understanding the seismic processes that drive seismicity. However, traditional methods for b-value estimation can be biased by subjective decisions, such as which earthquakes to include based on their magnitude. The B-GRASP project aims to overcome these limitations by developing an unbiased, probabilistic method for estimating spatio-temporal variations in b-value. Using original Bayesian approaches, the project will (1) investigate how the b-value changes over time during foreshock sequences and what this reveals about broader seismic processes of earthquake nucleation, and (2) produce updated b-value maps to improve PSHA in regions of low to moderate seismicity, such as Spain and France. The research is highly innovative, taking advantage of recent advances in the use of the b-value, and multidisciplinary, using Bayesian approaches for the first time to achieve better resolution of the b-value and its uncertainties. The results will ultimately be shared with the academic and private sectors involved in the production of seismic hazar
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Direct and indirect effects of Climate Change on biotic communities and exotic Pathogens in mixed Mediterranean forests' (CCPaMe) under Horizon 2020.

assistant: **Objectives**

Global change is a complex phenomenon that involves several factors such as climate change, the alteration of the nitrogen cycle, biological invasions or changes in land use. Although each of these factors has been individually studied in detail in the last years, it is still poorly known how they could interact to affect biotic communities and the ecosystem processes that they control. The general objective of this proposal is to advance in the understanding of the direct and indirect effects of climate change on biotic communities and invasive pathogens interactions in forest systems. This interaction has been largely ignored so far, but its consideration is crucial to understand how abiotic and biotic stress factors together determine forest health and stability. Specifically, CCPaMe aims to: 1) Determine the direct effects of climate change on the exotic pathogen Phytophthora cinnamomi. It will be identified potential non-additive effects of rainfall exclusion and warming on its abundance and infected capacity by field and growth chamber experiments. 2) Determine the indirect effects of climate change on soil microorganisms and P. cinnamomi ecology. It will be determined the effects of climate change on the hosts susceptibility to P. cinnamomi and on the abundance, diversity and composition of soil organisms community (bacteria, fungi, archaea and actinomycete) and their implications for P. cinnamomi functionality: modification of its infective capacity and the alteration of pathogen interactions with other soil microbes. CCPaMe results will provide key insights to address current challenges regarding climate change effects and exotic pathogen control on forest systems and the conservation and protection of forest resources. Moreover, they will allow an improvement
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'IMPROVED METHODS FOR THE DIAGNOSIS OF RUMINANT ALPHAHERPESVIRUS INFECTIONS IN RELATION TO THE CONTROL OF INFECTIOUS BOVINE RHINOTRACHEITIS (IBR)' demonstrating excellence, impact and implementation quality for a Fourth Framework call.

assistant: **Abstract**

Background :Bovine herpesvirus 1 (BHV-1) infection in cattle is a disease of major economic concern in Europe, both in countries where this infection has been eradicated and in those where the control of infectious bovine rhinotracheitis (IBR) is currently or will be undertaken. Successful control of IBR depends on the use of efficient, sensitive and specific diagnostic tests. The efficiency of the existing diagnostic methods is compromised by the existence of serological cross-reactivity between BHV-1 and the other closely related alphaherpesviruses. The objective is :The aim of this research is 1) to assess the risk of cross-infection between BHV-1 and other alphaherpesviruses in ruminants, and 2) to develop diagnostic methods capable of differentiating the different ruminant alphaherpesviruses infections.  Short description of the project :The work will be subdivided into 3 phases : - in vivo evaluation of cross-infections of cattle - serological differentiation of ruminant alphaaherpesviruses related to BHV-1 - genomic differentiation of ruminant alphaviruses related to BHV-1
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'Secure, Trusted and Legally Ruled Collaboration Environment in Virtual Life'.

assistant: **Objectives**

VirtualLife aims to provide an immersive and secure environment, combining a 3D virtual experience with the trustiness of a secure communication infrastructure.VirtualLife constitutes a new form of civil organization, finalized to the creation of secure and ruled places within the virtual world, where important transactions can occur. The aggregation in communities and the collaboration between users is encouraged in order to reach a management of common and private interests. This collaboration is achieved through the definition of common rules that take care of all the involved cultures. A standard collection of laws, the Virtual Constitution, finalized to the creation and regulation of a secure and trusted environment (Virtual Nation) will be studied.In order to reach high quality 3D metaverses the computing effort will be distributed on a peer-to-peer network with nodes connected using a secure protocol. Thus the resulting Virtual World will not be hosted on a central server cluster but will be based on a network of Virtual Zone Servers (VZ Servers). This gives the end-user the full control on the fundamental components of the whole world (Virtual Zone Server) and the protection of his sensible data.The peer-to-peer architecture will enable easy and fast sharing of contents without third ruling partners: the only ruling entity will be the law in force, defined by the users' community and accepted by those users who join the community afterwards.Each VZ Server simulates all the entities in the zone and gives the users the possibility to create and share contents, media, and data in a very intuitive way.VirtualLife constitutes a new form of virtual organization, representing the transition from existing Virtual Worlds administrator-centric to a civil organization rule