RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Integral large scale experiments on hydrogen combustion for severe accident code validation' demonstrating excellence, impact and implementation quality for a Fifth Framework call.

assistant: **Abstract**

Hydrogen posses a major threat to containment integrity in the case of a severe accident in a nuclear power plant. Reliable analysis of severe accident situations and optimisation of mitigation designs are essential for enhancing safety and avoiding unnecessary conservatism. To ensure this, the analytical tools being developed by many organisations world-wide must be validated on an experimental data base which covers relevant aspects of the hydrogen problem: sources, distribution, deflagration, detonation. The experimental and analytical work performed in this project contributes to the data base in the fields of slow to fast deflagration. It addresses especially the aspects of hydrogen combustion in complex, multi-compartment configurations on large, plant typical scale and with premixed, inhomogeneous gas mixtures. Advanced models, deflagration criteria and numerical tools developed by the partners will be validated against experiments which will result in qualified tools with well defined ranges of applicability for hydrogen risk assessment of nuclear power plants.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Mind the Gap: from Plasma Kinetics to Cosmological Galaxy Formation' (PICOGAL), addressing dissemination and exploitation.

assistant: **Impact**

In the modern picture of galaxy formation, baryonic feedback is critical for shaping galaxies and regulating star formation. On small scales, feedback results from transporting momentum, radiation, thermal and relativistic particles but current-day magneto-hydrodynamic simulations of galaxies and galaxy clusters often neglect or over-simplify these transport processes. Electrons transport heat and cosmic rays exchange momentum and energy with the thermal plasma but both species are erroneously assumed to diffuse along magnetic field lines. However, this is in conflict with the latest plasma simulations and observations in the solar wind and of the galactic center, which imply efficient wave-particle scatterings so that the electrons and cosmic rays are advected with whistler and Alfvn waves, respectively. We propose a coordinated multi-scale approach that combines plasma kinetic and global fluid models of particle acceleration and transport in galaxies and galaxy clusters with unprecedented accuracy. In particular, we will run novel plasma simulations of shocks at supernovae and galaxy clusters, and study the plasma-wave mediated transport of electrons and cosmic rays. We will employ information field theory to coarse grain these models to derive effective transport coefficients, which will be implemented in macroscopic fluid models of cosmic ray transport and thermal conduction. Simulating feedback by cosmic rays, radiation and supernovae in cosmologically forming galaxies on scales from dwarfs to our Milky Way provides transformative changes of the physics accuracy  of these models. This is complemented by cosmological galaxy cluster simulations with improved physics to understand the origin of the cluster-core bimodality, giant radio relics and halos. Comparing mock
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'A single-molecule view of initial transcription' to be submitted under Seventh Framework.

assistant: **Abstract**

The overall objective of this project is to study the initial phase of gene-transcription – i.e., the transformation of genetic information from DNA to RNA – at the level of individual molecules. State-of-the-art single-molecule fluorescence spectroscopy will be used in combination with a nanoscale “spectroscopic ruler” (a method based on the phenomenon of fluorescence resonance energy transfer, also known as FRET) to capture the transient intermediates and conformational changes of RNA polymerase, the protein machine that orchestrates transcription. This analysis will complement the static snapshots of transcription complexes, which have been obtained using X-ray crystallography. Single-molecule fluorescence methods are well suited for real-time studies of initial transcription, since the available temporal resolution is sufficient for monitoring conformational changes during the addition of a single nucleotide to an RNA chain. The specific aims are to develop novel real-time assays for detecting promoter-DNA opening and promoter-escape in vitro; to use the in vitro assays for studying the effect of specific DNA sequences on promoter-proximal pausing and promoter escape; and to take the first steps towards developing similar single-molecule FRET assays that report on transcription in living bacterial cells. The proposed work will contribute to the understanding of initial transcription, providing insights applicable to the transcription systems of higher organisms such as humans. The proposed toolbox will also find extensive use in the study of other important protein-DNA interactions, both in vitro and in vivo.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Microscopic Processes and Phenomena at Oxide Surfaces and Interfaces' (OXIDESURFACES).

assistant: **Innovation**

Metal oxide surfaces and interfaces play a key role in energy-related applications and in novel schemes for electronic devices that exploit the special physical and chemical properties of these promising materials.For progress in both areas, a detailed, mechanistic understanding of the atomic and molecular processes that occur at oxide surfaces and interfaces is critical.  Experiments on well-characterized model systems in conjunction with computational modelling can provide such insights, but current investigations are limited in the range of materials and scope of phenomena that can be studied, and to experiments in a low-pressure environment.Research conducted in this project will push these limits by:•Developing new methodologies for atomic-scale investigations of the subsurface region of oxides with mixed electronic and ionic conduction to measure mass and charge transport across oxide interfaces.•Combining cutting-edge molecular beam epitaxy techniques with atomically-resolved scanning tunneling microscopy to synthesize samples of multi-component metal oxide materials with tailored surface properties.•Establishing a new research thrust that will combine both ex-situ and in-situ electrochemical surface science techniques to study structurally well characterized metal oxide surfaces in an aqueous environment.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Manipulating tumour suppression: a key to improve cancer treatment' demonstrating excellence, impact and implementation quality for a Sixth Framework call.

assistant: **Abstract**

The prevention of human cancer development depends on the integrity of the cellular defence mechanisms that respond to stress.A key player in this defence is the p53 tumour suppressor, which eliminates cancer cells by inhibiting their growth.These p53 functions often affect the efficacy of anti-cancer therapies.While p53 is often mutated in human cancers, it remain normal in about 50% of cases, notably in breast carcinoma. the non-mutated p53 could be activated to suppress tumour progression.Deregulation of p53 modulators, such as Mdm2 or p73, can hampered p53 tumour suppression activities by acting upstream and/or downstream of p53.It is crucial to understand how p53 modulators contribute to human malignancies.Based on this information, we propose to develop rational theraupetic approaches to manipulate p53 modulators,thereby wakening the sleeping tumour suppressive activities of p53, allowing it to eliminate cancer cells.A carefully structured consortium will interactively build a technology platform to comparatively identify,characterize and evaluate the regulatory roles of p53-modulators and define the mechanisms of their action.Large-scale gene functional analyses will be conducted to identify relevant signalling pathways that impair or mediate tumour suppression by p53.These analyses will include p53 activators and inhibitors, p53 homologues p73/p63,and dissection of p53 targets genes mediating apoptosis and growth arrest.Our links with highly profiled clinical partners and our access to large, well-characterized and clinically documented sample collections will enable the evaluation of diagnostic expression profiles, and their potential prognosis value in cancer.Particular emphasis will be directed towards translating basic knowledge of functional oncogenomics in
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Decoding Plant-Microbiome Interactions at Single-Cell and Spatial Resolutions' will create measurable scientific, societal and economic impact aligned with Horizon Europe.

assistant: **Impact**

Plant-microbiome interactions are crucial for plant health, promoting growth and enhancing resilience under stress conditions. These interactions are highly heterogeneous, shaped by diverse plant cell types and the complex spatial distribution of microbes. However, our current understanding remains predominantly at the tissue level, lacking the resolution to fully capture this heterogeneity. As a result, the genetic networks that govern how plants foster beneficial microbiomes remain largely unexplored. This Fellowship aims to address these gaps by employing cutting-edge single-cell multiomics and spatial mapping technologies. Using Arabidopsis thaliana and the well-characterized 35-member synthetic microbial community (SynCom35) as model systems, I will integrate single-cell transcriptome and chromatin accessibility assays to map transcriptional and epigenetic changes in root cells in response to microbial colonization. This approach will provide novel insights into the cellular mechanisms and genetic networks underlying plant-microbiome interactions. Additionally, by utilizing and further developing PHYTOMap, a multiplexed 3D spatial gene expression analysis platform, I will simultaneously spatially map plant gene expression and microbiome distribution. By integrating single-cell and spatial omics data, I will reconstitute spatially-resolved plant gene regulatory networks that interact with specific microbiome members. Furthermore, I will functionally characterize these predicted regulatory networks, aiming to lay the foundation for genetic engineering to enhance plant interactions with beneficial microbes, offering new strategies for optimizing plant growth and resilience. Altogether, this research will significantly advance our understanding of plant-microbiome inte
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Transcriptional Regulation Assessed in Neuronal Subtypes in three major interrelated Psychiatric disorders' (TRANS-PSYCH) under Horizon Europe.

assistant: **Objectives**

Schizophrenia (SCZ), bipolar disorder (BP), and Major Depressive Disorder (MD) are three major psychiatric disorders that affect mood, thinking, and behavior. These disorders are strongly genetically intercorrelated, and exhibit pronounced clinical overlap, suggesting that they are different manifestations of a shared underlying neurobiology, along a spectrum. However, the neurobiological mechanisms and pathophysiology of these disorders are still poorly understood, limiting effective drug discovery. There is an urgent need for new models for drug testing, as animal models have major limitations, and current psychiatric organoids systems are dependent on patient derived stem cells, in which technical, genetic and biological diversity confound the interpretation, and obscure the underlying neuropathology. The major challenge of this ERC proposal is to develop organoid models for psychiatric disorders by targeting upstream transcription factors. Transcription factors are key molecules that drive cell type differentiation including neuronal network formation. Hence, the central hypothesis of this ERC proposal is that neuronal subtype associated transcription factors can function as targets to model these disorders using brain organoids. However, the neuronal transcriptional regulators that mediate these disorders are currently unknown, and difficult to identify. Novel genomics technologies allow for an unbiased characterization of the brain in order to detect cell types and genes that are dysregulated in disease, and can be used to identify putative upstream transcription factors. Here, I propose a selection of multi-omics profiling - strategies to a unique collection of high quality psychiatric human brain tissue aimed at reliably identifying key upstream transcription fa
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Green SELf-Powered NEuromorphic Processing EnGines with Integrated VisuAl and FuNCtional SEnsing' to be submitted under Horizon Europe.

assistant: **Abstract**

Today’s Internet-of-Things (IoT) incorporates a complex distributed network of wireless sensors and processors connected to the cloud. These platforms and their data-processing needs are creating a stronger and stronger demand for energy that is not sustainable. At the same time, the increasing consumers demand for IoT electronic devices and their limited lifespan, are significantly contributing to the world’s fastest-growing waste stream, known as electronic waste. To avoid an unsustainable energy cost in this data deluge, disruptive innovations in electronics from material to systems are urgently required. ELEGANCE proposes the development of a radically new, printable and light-operated processing technology specialized for IoT edge-computing applications. The project implements an eco-sustainable approach at component and processes level, where abundant, recyclable eco-friendly materials are employed, targeting a zero environmental footprint strategy. The processor’s building block includes a hybrid stack of an oxide optoelectronic memristor with an electrochromic layer on top exhibiting a unique light-triggered Processing-in-Memory enabling simultaneous IoT energy-efficient computing and visual sensing. In-memory computing schemes, such as crossbar memristor arrays, will be implemented employing low-cost, industrially compatible sustainable printing techniques. This will enable the design of energy efficient neuromorphic and artificial intelligence computing systems optimized for a plethora of consumer applications in the wearable, healthcare, and edge-computing sectors, making ELEGANCE an ambitious and technologically concrete breakthrough for the IoT with high potential for large societal benefits.


**Keywords:** oxide transistors, memristors, electrochromics, 2
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Blocking SUMO conjugation as drug discovery strategy.' (SUMOblock).

assistant: **Concept**

In eukaryotes, SUMO (Small Ubiquitin Modifier) conjugation to proteins is an essential process that regulates many aspects of cell biology. Defects in SUMO conjugation have been associated to cancer, neurodegenerative diseases and others, raising a great interest in developing novel drugs for inhibiting SUMO conjugation.Specifically, recent reports have pointed to a high dependency on an active SUMO conjugation by Myc-overexpressing cancers. Consequently, SUMO conjugation has become a non-oncogene addiction target of special relevance since the oncogenic Myc is nondruggable. Based on the results obtained by our group, we have developed a novel assay for identifying efficient SUMO conjugation inhibitors, which could lead to the identification of novel therapeutic drugs.The main outcome that we expect to obtain from this project is the identification of a SUMOylation inhibitor that will be patent protected. Initial steps towards the valorization of the identified product will be taken, and available commercialization opportunities will be carefully analyzed for entering into the next steps of drug development, including preclinical and clinical studies.The fact that SUMOylation may be a key element in almost 70% of cancer types due to its involvement in the Myc pathway makes the search for hit drug candidates an enterprise worth taking, which could benefit millions of people worldwide.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'A transcriptomic approach to understand the avian genetic susceptibility to West Nile Virus infection' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

Emerging infectious diseases that have a wildlife reservoir pose a major challenge to global health. This is the case for West Nile Virus (WNV), which is the number one causative agent of viral encephalitis worldwide. Although it infects humans, the main hosts of WNV are birds. Thus, to develop effective intervention strategies it is critical to understand the disease dynamics in birds. In WNV, disease dynamics are influenced by variation in disease susceptibility across individuals and species, but the factors underlying this variation are poorly understood. Here, I propose to focus on one of these factors: the genetic basis of the immune response. Specifically, I will characterize the transcriptional response to WNV infection and identify candidate genes and pathways that will allow us to gain insights into the avian immune response to the virus and uncover the genetic basis of susceptibility both at the species and individual level. To achieve this goal, I will first analyze the transcriptional response in individuals of house sparrow that die and individuals that survive a WNV infection and test for differential expression. Second, I will characterize the transcriptional profile of house sparrows inoculated with 4 different strains of WNV.  Finally, I will examine transcriptional profiles and test for differential expression in 3 species (house sparrow, Eurasian collared dove and common quail) with different susceptibility to WNV. To complete this work I will use state-of –the-art genomics, bioinformatics and statistical analyses and will integrate knowledge from genomics, disease ecology and immunology. Obtaining a Marie Sklodowska-Curie fellowship to carry out this project under the supervision of Prof. J Figuerola (Doñana Biological Station) will be a unique oppo
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Meiotic crossing-over: from spatial distribution to in situ chromosomal architecture' under Horizon 2020.

assistant: **Concept**

To haploidise their genome, sexually reproducing organisms employ a specialised cell division program – meiosis – which consists of one round of DNA replication followed by two consecutive rounds of chromosome segregation: meiosis I and II. While meiosis II resembles mitosis, the ability of cells to segregate homologous chromosomes entails several specialised events. In most organisms, physical linkage and subsequent disjunction of maternal and paternal chromosomes require homologous recombination and crossing-over. As observed over a century ago, crossovers occur at different chromosomal positions in different meiotic nuclei – however, the incidence of a crossover in a given location reduces the probability of a neighbouring crossover event. As a result, crossovers tend to be widely and evenly spaced along chromosomes, a phenomenon termed crossover interference. Work in the last 30 years has led to remarkable progress in the delineation of the sequence of molecular events that lead to crossing-over. However, how cells spatially regulate the deployment and assembly of molecular determinants to accomplish crossover patterning remains largely unknown. Here, I propose to tackle this fundamental question through the development of two novel approaches tailored to explore central aspects of meiotic recombination with unprecedented resolution:  i) to understand how chromosomal context shapes crossing-over, we will develop novel methodology (HJmap) to achieve genome-wide mapping of Holliday junctions: central recombination intermediates which mark future crossover sites.  ii) to explore how local chromosomal features influence crossing-over, we will visualise the architecture of crossover-designated recombination intermediates in situ, and in 3D, using electron cryotomography.