RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Dissemination  of proneural brain cancer cells: implication of partial and reversible differentiation into Dcx+ cells' in the context of a Seventh Framework call.

assistant: **Objectives**

Gliomas describe a range of devastating and progressive brain tumours affecting around 25000 people each year in Europe. Glioblastomas represent the most severe and frequent form of gliomas. A major finding in the field of glioblastomas is the recent identification of cancer stem cells within the tumour, holding a high tumorigenic potential. Those stem cell-like cancer cells are multipotent and resistant to radio- and chemotherapy, and are thus very likely to be responsible for the tumour recurrence after surgical resection. They possess as well high migratory properties, strongly understudied so far. Their anarchic dispersion through the parenchyma of patients represents the main hurdle to surgical resection therapy, and their ability to re-generate a tumour threatens the therapeutic outcome. That is why a deep understanding of the mechanisms preferentially borrowed by glioma stem cells to spread and survive should lead to adapted therapeutic strategies. This project is based on the original discovery by JP Hugnot’s group that glioma cells can spontaneously express the neuroblast marker Dcx to enter a migratory program in vitro, and in vivo. Interestingly, Dcx+ and Dcx- glioma cells from the same culture share different migratory and tumorigenic properties. This project aims at deciphering the respective intrinsic properties of Dcx+ and Dcx- gliomas cells in terms of migration and tumorigenicity, and to identify which molecular pathways influence these properties (with a focus on Notch and Shh based on preliminary data). For that, several resources are already available and in Pr Hugnot’s group: cell sorting, gliomas cell lines reportering the expression of Dcx (Gli4 pDcx-GFP), migration assays, cell transplantation of Dcx+ or Dcx- cells in the brain of nude mice.When
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Characterization of the mechanism of inter-organ communication coordinating tissue growth and developmental timing' (CoordinGrowthDev) under Horizon 2020.

assistant: **Objectives**

Over the recent years, research in developmental biology has stumbled upon the problem of how development ensures that organisms achieve appropriate body and organ size. The coordination of organ growth with the developmental program is essential for the emergence of adults with proper body size and proportions. These species-specific features condition many traits of adult life such as fitness and survival. Studies on fruit flies have allowed setting up the conceptual frame for this research. Our current understanding is that tissue growth and the developmental program are synchronized by feedback control mechanisms that block the transition to the next developmental period until all tissues have completed growth.Recent work in Drosophila has started unraveling the nature of the signals that couple tissue growth with developmental progression. A newly discovered Drosophila insulin-like peptide called Dilp8 is key in this coupling, raising the possibility that peptides with similar roles may exist in vertebrates. The current project aims at characterizing the molecular mechanisms by which Dilp8 coordinates development of the different body parts. First, I will conduct a biased genetic screen to identify the receptor for Dilp8. This will allow a better characterization of the biological functions of Dilp8. On one hand, I will unravel the target tissues of Dilp8 mediating regulation of steroid hormone and developmental transitions. On the other hand, I will investigate the role of Dilp8 signaling in coordinating growth among developing organs.The project will shed light on the mechanisms of inter-organ communication that ensure the coordination of growth between tissues and with the developmental timing. The expertise of the host laboratory, combined the conceptual frame
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'The impact of 3D regulatory landscapes on the evolution of developmental programs' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

Vertebrate genomes are organized in 3D regulatory landscapes that control pleiotropic gene expression. Within them, transcription emerges from a precise interplay between genes, regulatory elements and 3D chromatin organization. We and others have shown how mutations in these regulatory layers can modulate phenotypes. However, how 3D regulatory landscapes shape the evolutionary history of developmental programs is still largely unexplored.Our limited capability to read, interpret and modify genomes has constrained our knowledge on how gene regulation evolves. However, recent advances in long-range sequencing, single-cell, chromatin interaction and genome editing methods offer novel tools to link genomic variation and phenotypes. Here, I will combine these methods to investigate how changes in 3D regulatory landscapes contribute to refine the function of developmental programs. As a testbed, I will study gonadal sex determination, an essential process for species perpetuation but characterized by a remarkable evolutionary plasticity.We will use single-cell transcriptomics across tetrapod clades to unravel the core and variable transcriptional network of sex determination. Single-cell epigenomics and novel chromatin interaction methods will reconstruct 3D regulatory landscapes and infer sources of regulatory variation. With phylogenomic comparative strategies, we will estimate the contribution of different types of mutations to different sex determination programmes, as well as the impact of whole genome duplications. Finally, we will develop innovative transgenic methods for inter-species replacement of 3D regulatory landscapes, using this technology to activate early estrogen synthesis in mouse. 3D-REVOLUTION will provide ground-breaking insights on the evolution of gen
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Role of astrocytes in Huntington's Disease: characterization of a novel mouse model with targeted expression of mutant huntingtin in the striatum' (ASTRO-HD).

assistant: **Innovation**

Huntington’s disease (HD) is an inherited neurodegenerative disease, which has no cure, resulting from the mutation of the gene coding for huntingtin protein (htt). Clinically, HD patients present with progressive involuntary spasmodic movements and cognitive impairments. Post-mortem studies of HD patients reveal severe atrophy of the striatum, reflecting a selective neuronal loss of medium spiny neurons (MSNs). The path leading to MSNs death remains unknown. Conversely, astrocytic dysfunction might be a causal factor leading to HD pathogenesis. Astrocytes play 2 key roles in neuronal survival implicating glutamatergic transporters: supplying energy to the neurons (through a glycolytic flux) and regulating synaptic activity (mainly glutamatergic); and yet, both glucose consumption and glutamate regulation are altered in HD. To further study the specific role of astrocytes in the pathogenesis of HD, the host laboratory developed, using a lentiviral–based approach, the first in vivo mouse model which selectively expresses mutant htt in astrocytes. The main objective of this proposal is to gain new insights into the specific effect of astrocyte mutant htt expression on glutamate transporters and glycolytic metabolism. This objective will be achieved by pursuing a comprehensive characterization of this novel model (behavioral and neuropathological analyses using a stereological approach and detailed cell morphology analysis) and monitor the astrocytic function by combining techniques mastered by the applicant (electrophysiology) and by the host laboratory (glutamate receptors regulation and glucose utilization). Furthermore, defining the implication of altered astrocytes on neuronal loss would shed the light on the role of astrocytes in other neurodegenerative diseases.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Revealing the function of dormant soil microorganisms and the cues for their awakening' to be submitted under Horizon 2020.

assistant: **Abstract**

Soils are considered the last scientific frontiers that harbor one of the most diverse microbial communities on Earth. It is hypothesized that this diversity allows for redundancy in microbial key processes, thereby ensuring ecosystem stability. Much of this functional redundancy is embodied in non-active, dormant microorganisms that represent the ‘microbial seed bank’, which is characterized by a high number of low abundant taxa. Based on the recent theory of a ‘dynamic rank-abundance curve’, it is hypothesized that the rare dormant organisms can be recruited to participate in a given function upon resuscitation with environmental cue(s). In this project I will test this hypothesis on a level that matters for ecosystem processes – the functional level – by an innovative approach combining stable isotope probing (SIP) and sequencing with process-level and single-cell activity analysis.By testing 4 hypotheses, we will (1) reveal environmental cues that resuscitate dormant microorganisms involved in major soil functions and identify the activated microorganisms. The activity of the resuscitated communities will be analyzed at the process level, as well as at the single-cell by NanoSIMS, thereby allowing us to elucidate the impact of dormancy/resuscitation dynamics on targeted processes at the population and ecosystem level. (2) We will investigate the genetics of microbial dormancy-resuscitation strategies in a natural model environment for dormancy, an arid ecosystem, by metatranscriptome analysis of critical dormancy-resuscitation steps. (3) We will retrieve genomic information of primarily rare, but after resuscitation active, microorganisms involved in important soil processes, as they presumably contain so far unknown genomic potential. In summary, this project will
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Fire-induced changes on surface soil microbial communities in Mediterranean forests. Rehabilitation by addition of exogenous organic matter' (BURNINGMICROBES).

assistant: **Innovation**

Over 40.000 wildfires occur yearly in Mediterranean Europe causing devastating environmental damages: greenhouse gas emission, habitat destruction and accelerated desertification. Burning can sterilize the soil surface within minutes, whereas the recovery of the decomposer system and its ecosystem functions can take decades. Our knowledge on the effects of fire on the decomposer system mostly relies on surveys performed in humid climates. Thus, it is intended in this proposal to survey the fire-induced changes in the soil microbiota in Mediterranean forests. In particular we aim to: i) Analyse the effects of an experimentally controlled wildfire in a Mediterranean shrubland on the soil´s physical and chemical properties, as well as microbial biomass (microbial C content), activity (ATP content and respiration), ability to consume a range of C substrates (MicroResp), and several hydrolases linked to the C, N and P cycles, ii) Investigate fire as an ecological filter selecting for endosporulating bacterial groups. We will analyse the bacterial community structure (denaturing gradient gel electrophoresis of 16S rRNA gene amplicons) in pre- and post-fire samples. Based on these results, adequate samples will be selected for generating clone libraries to construct the phylogeny of the regional bacterial community. We expect that endosporulating bacteria, with their higher resistance to heating, will be overrepresented in the post-fire community, what will be analysed using the picante library of R. If demonstrated, this would imply the loss of phylogenetic diversity, which is the key component of biodiversity determining the ecosystem functioning, iii) Evaluate the restoration of soil properties after applying exogenous organic matter. We will investigate in a microcosm expe
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Past and Future High-resolution Global Glacier Mass Changes' under Horizon Europe.

assistant: **Concept**

World-wide glaciers are losing mass which affects global sea-level, river runoff, freshwater influx to the oceans, glacier-related hazards, and landscape changes, with implications for human livelihoods and ecosystems. Hence, accurate estimates of past, current and future glacier mass variations at a high temporal and spatial resolution are key to effective adaptation strategies. However, previous mass-balance reconstructions and projections have relied on scarce observations with limited spatial and/or temporal resolution, as well as overparameterized, insufficiently constrained and highly simplified models, the latter necessitated by high computational costs incurred by the global scale.GLACMASS will propel the current state-of-the-art of global-scale glacier reconstruction and projection forward in unprecedented ways by delivering a fundamentally novel and internally consistent physically-based modelling framework that draws, for the first time on a global scale, on both data assimilation and modern machine learning techniques facilitated by emerging global-scale glacier-related satellite-derived data. The framework will be used to reconstruct multi-decadal past glacier changes, and make policy-relevant multi-century projections of mass and area changes of all >200,000 glaciers outside the ice sheets with unprecedented accuracy, spatiotemporal detail and computational efficiency, and also nowcast present mass changes in a near-real-time fashion for selected regions. The model framework will fuse output from a novel physically-based glacier evolution model with all relevant observations available for each glacier, such as in-situ, geodetic and gravimetry-derived mass balances, as well as snowlines and other observations, thus simultaneously exploiting the untapped str
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Full-Duplex Radios for Local Access' (DUPLO), addressing dissemination and exploitation.

assistant: **Impact**

Future development of wireless communications networks will require radical improvements over the existing radio transmission and network solutions to support tremendous growth of data traffic in future networks in an energy-efficient manner.The project Full-Duplex Radios for Local Access (DUPLO) aims at developing new technology and system solutions for future generations of mobile data networks by introducing a new full-duplex radio transmission paradigm, the same carrier frequency is used for transmission and reception at the same time. The approach holds the potential to significantly increase the capacity, and will focus on energy efficient solutions which also enable a more flexible use of the spectrum.DUPLO will study and develop technical solutions for efficient self-interference cancellation in wireless transceiver to enable usage of full-duplex transmission in different nodes of the wireless communications networks, including access points, devices and relay nodes. Attainable gains of full-duplex transmission and required system level solutions to accommodate the new transmission mode in wireless communications networks will be investigated. The main focus will be in small radio cell operation environment. Novel solutions for antenna, RF and baseband domain self-interference cancellation will be analyzed and developed. The most promising concepts will be implemented in a test hardware circuitry. A proof-of-concept test platform will integrate the developed technical solutions together into functional full-duplex communication system, which can be used in testing and further development of novel solutions.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Integration of retinal inputs by distinct collicular cell types' in the context of a Horizon 2020 call.

assistant: **Objectives**

Animals have a diverse set of behaviors that are triggered by specific sensory stimuli, such as motion or looming. In the visual system, this process begins in the retina where the visual scene is divided into 20 parallel information channels before reaching the brain. The superior colliculus is one of the main recipients of retinal output and it mediates tractable visually-guided behaviors such as eye movement, orienting or escaping behaviors. However, it remains unknown how visual signals from individual retinal ganglion cell types are processed by neurons in the superior colliculus to achieve specific computations relevant to behaviors.To answer these questions, I will use transgenic mouse lines recently identified by the host laboratory, in which specific types of retino-recipient neurons of the superior colliculus are labeled with Cre recombinase. First, I will characterize the response properties of Cre-labeled individual cell types using in vivo two-photon calcium imaging during visual stimulation. Next, I will initiate calcium sensor-functionalyzed trans-synaptic viral tracing from Cre-labeled collicular cell types, and perform two-photon calcium imaging of labeled presynaptic retinal ganglion cells and starter collicular neurons. With this approach I will relate the activity of neurons to the activity of connected neuronal networks, and evaluate the degree of convergence and divergence in retino-collicular connectivity.By linking cell types, circuits and computations, this work will provide mechanistic insight into the circuit basis for parallel processing of visual information and various visual functions in the healthy system, while possibly disclosing novel therapeutic targets for visual motor diseases.


**Keywords:** superior colliculus, retinal ganglion c
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Reactive molecular dynamics for Organometallic reactions relevant to asymmetric hydrogenation reactions' (2002-2006), addressing dissemination and exploitation.

assistant: **Impact**

Asymmetric catalytic hydrogenation is a reaction of great interest in total organic synthesis and with valuable applications in the pharmaceutical industry. This project will apply and extend recent developments in the state-of-the art for reactive force fields and reactive molecular dynamics (MD) to the simulation of iridium-catalyzed asymmetric hydrogenation reactions recently developed by Pfaltz and co-workers at Basel. The initial step will be the parameterization of the ReaxFF force field for iridium, based on a library of DFT structures and energies for a variety of conformations and transition structures, based on Pfaltz's asymmetric hydrogenation phosphinooxazoline (PHOX) catalysts. Once the force field is ready, it will be used for running extensive conformational searches using Monte Carlo and molecular dynamics methods, for a variety of ligands and substrates; the most promising structures will be refined using more accurate DFT methods. This will be followed by reactive MD simulations of the react ion process itself, with inclusion of solvent effects. The result will be a detailed understanding of the reaction mechanism, and will ultimately facilitate the screening of new ligands and substrates for the hydrogenation reaction. The project will involve a close collaboration with Prof. Pfaltz, who will provide additional experimental data for calibrating and testing the methods, and who can test our predictions.