RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'In vitro reconstitution of TTSS-mediated export across the bacterial inner membrane of Shigella flexneri' will create measurable scientific, societal and economic impact aligned with Sixth Framework.

assistant: **Impact**

Shigella flexneri, a Gram-negative enterobacterium, causes dysentery in humans. It uses a type III secretion system (TTSS) to infect its host. The TTSS is a large protein complex that spans both bacterial membranes and the host plasma membrane. It mediates the apparent direct translocation of proteins from the bacterium through its central channel into the host cell. Therefore, unravelling the molecular mechanisms by which the TTSS functions is key to understanding how it mediates this host-pathogen interac tion. For this purpose, we aim to reconstitute in vitro its sole energy-dependent step, i.e. protein export across the bacterial inner membrane (IM). In vitro reconstitution allows us to study features of the protein export reaction that would otherwise no t be accessible, e. g. analysing the separate steps that encompasses the passage of the substrate through the IM channel.First, we will analyse the in vivo properties of protein export by the IM domain, using a Shigella strain that lacks the periplasmic/ou ter membrane region of the TTSS. In this strain, the export of MxiHCdelta5, a non-polymerising mutant of the needle protein MxiH, will be analysed. Then, the components of the IM domain of the TTSS will be overproduced and inner membrane vesicles (IMVs) ha rbouring the overproduced IM domain will be isolated. Protein export assays will be conducted in vitro by the addition of the cytoplasmic TTSS components and the substrate MxiHCdelta5. These results will be compared to the data acquired in the in vivo expo rt assay. These experiments will greatly enhance our understanding of TTSS function. Many elements of TTSSs are evolutionary conserved, in particular the IM domain, thus our work should also help understand how the TTSSs in bacteria such as Chlamydia, Salm on
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Deciphering Bacteria-induced Morphogenesis and Protection in marine Eukaryotes', explaining its relevance to EU policy priorities under Horizon 2020.

assistant: **Impact**

Symbiotic bacteria play critical roles in animal evolution, development and metabolism. The molecular and cellular mechanisms underlying these fundamental interactions, however, are largely unknown. To fill this major knowledge gap, I will establish the bacteria-Hydractinia symbiosis as a new model system to fully characterize key cross-kingdom signalling molecules and response mechanisms. The results of my ERC proposal (MORPHEUS) will lead to ground-breaking insights into molecular drivers of eukaryotic morphogenesis, illuminate the evolutionary history of developmental signals for animals – including humans – and provide new chemical scaffolds with intrinsic biological activities that are urgently needed for drug discovery.The marine colonial hydroid Hydractinia belongs to an early branching metazoan lineage, dating back more than 500 million years. The organism reproduces through a larval stage, which upon perception of yet unidentified bacterial morphogenic signals, produced within marine bacterial biofilms, undergoes transformation into the mature organism. In the absence of the bacterial signals, the larva fails to settle and eventually dies. This fundamental process is the basis of this proposal. Capitalizing from my recent pioneering work, I will address the following pressing research questions: Which bacterial signals ensure larval recruitment and metamorphosis? How are bacterial signalling molecules perceived? How is the system protected against alien species? I will apply an innovative combination of state-of-the-art methodologies developed within the fields of natural product and synthetic organic chemistry, microbiology and molecular biology to pursue an in-depth biochemical analysis of this paradigmatic system. Results of MORPHEUS will be transformative f
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Resource-based growth of new, technology-based firms - new theory and its empirical application' demonstrating excellence, impact and implementation quality for a Fourth Framework call.

assistant: **Abstract**

RESEARCH QUESTIONS 1 What factors determine the growth potential of new, technology-based firms (NTBFs)? 2 What are the optimal strategies for realizing this growth potential? CONTENT 1 Develop a new resource-based theory for the growth of NTBFs 2 Test the theory in regional agglomerations of NTBFs in Europe Development of metrics for appropriability, sustainability, durability, complexity, tactiness to empirical surveys of NTBFs (Helsinsky and Oulu: Finland, Cambridge and Oxford;UK) 3 applications of real theory for assessing growth and value potential of NTBFs for litterature survey and experts interviews. TRAINING IMPACT I work with leading researchers in a leading European research institution in the area to strenghten research skills in venture capital and NTBF finance (work wirh Hay, Sapienza, Prevezer, Baptista) help the applicant to become further integrated in the international research community. The applicant will be able to transfer his experience back to his home institution, thus contributing to the European cohesion. INDUSTRY LINKS The research is carried out in close contact with NTBFs. The results are made available for participating NTBFs so that they can immediately apply them. . . h h Complementary resource Value realization  availability strategy  Working model TASKS 1 development of metrics for appropriability, sustainability, durability, complexity, tacitness 2 empirical surveys of NTBFs (Helsinki and Oulu: Finland; Cambridge and Oxford; UK) 3 application of real option theory for assessing growth and value potential of NTBFs 4 literature survey and expert interviews TRAINING IMPACT 1 work with leading entrepreneurship researchers in a leading European research institution in the area 2 strengthen research skills in venture capital and NTBF financ
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Flow network morphology as memory map: Principles of fluid flow driven dynamic memory in living tubular networks' (FlowMem) under Horizon 2020.

assistant: **Objectives**

Fluid flows through tubular networks are crucial for life as they are the dominant means of substance and signal transport. In living networks – across organisms as disparate as animals and fungi, alterations of flows drive dynamic adaptation of tube diameters which in turn alters transport performance. In effect, local transient stimuli that affect flows are memorized as long-lived alterations to tube diameters across the network. I aim to identify the physical principles behind fluid flows driving dynamic memory storage in network morphology. I will thereby uncover how to control network morphology and performance by applied flow-altering stimuli, which promises significant advances in important challenges of the future: treatment of vascular diseases and tumour development, encoding complex behaviour in soft robotics and self-optimizing porous media.The dynamic nature of flows and networks’ complex morphologies requires a combined experimental and theoretical approach to address: What are the physical mechanisms of how flows in living tubular networks can encode and store information about stimuli? How do memories impact network performance? As experimental model system I choose the slime mould Physarum polycephalum. It is ideally suited as a starting point, as it reduces the problem in its complexity to just a tubular network. This model allows me to follow with unprecedented level of detail how stimuli transiently perturb network-wide flows – flows that subsequently drive long-term changes in network morphology. Theoretical models will verify mechanisms and allow investigation of impact on network function. Identified principles of dynamic memory formation will be applied to study consequences of mini-stroke stimuli and possible treatment in brain microvasculature
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon 2020 proposal for the project 'Tweaking dreams: non-invasive modulation of the level and content of mental activity during sleep'.

assistant: **Objectives**

Sleep and wakefulness have traditionally been regarded as two mutually exclusive states characterized by differences in consciousness and responsiveness to the environment. However, the last two decades of research have demonstrated that sleep is actually a locally regulated phenomenon and that cortical islands of sleep- and wake-like activity can often coexist across distinct brain areas. Intriguingly, this mosaic of activity is also directly related to the presence and content of mental activity during sleep. In line with this, many sleep disorders, including insomnia and arousal disorders, are associated with significant local alterations in the balance between wake- and sleep-like activity. In spite of these considerations, the classical view of sleep as a uniform global state is still dominant in both basic and clinical research. Moreover, it remains unclear whether the occurrence of local wake-like activity is related to specific physiological functions of sleep.The objective of this project is to progress towards a deep understanding of the mechanisms that regulate sleep at a local level through the exploitation of known properties of the thalamocortical system. At the core of the proposal is the idea that particular sensory-stimulation protocols may allow to directly modulate sleep intensity in a local, region-specific manner. Such approaches could be used to non-invasively perturbate regional sleep-related brain activity, thus allowing to investigate the causal consequences on sleep mentation, subjective sleep quality and sleep-related functions, including learning and memory. Of note, the same approaches could also find application in counteracting alterations of local sleep regulation in pathological conditions.Knowledge gathered within the project could yiel
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'The Disobedient Brain: The social neuroscience of non-compliance to immoral orders' will create measurable scientific, societal and economic impact aligned with Horizon Europe.

assistant: **Impact**

The core goal of this project is to unravel the mechanisms of disobedience. To understand the factors that prevent an individual from complying with immoral orders, research should focus on two critical axes: (1) which social and situational factors support disobedience and (2) which individual traits and neuro-cognitive processes support disobedience. While the first axe has been extensively addressed in past literature, the second axe has scarcely been approached. DISOBEY is uniquely focused on developing a social neuroscience approach to understanding the mechanisms through which resistance to immoral orders may develop in a given situation. Two main neuro-cognitive processes will be considered: the sense of agency that one experiences when performing a voluntary action and the empathic response towards others’ pain. The main hypothesis is that individuals who retain a high sense of agency and empathy for pain under coercion are more likely to resist immoral orders despite potential social costs. To validate this hypothesis, MRI and neuromodulation techniques will be used. The project will include WEIRD (Western, Educated, Industrialized, Rich & Democratic) adults, as well as very unique and rare non-WEIRD populations, such as military, genocide perpetrators, survivors and their offspring in Rwanda, and ‘Righteous’ individuals who rescued lives from extermination during genocides. Including these populations will also allow to validate my hypothesis beyond WEIRD societies, with individuals who actually experienced the disastrous consequences of obedience during real-life events. With this project I aim to pioneer a new area of research that will have deep societal implications. In the future, I intend to develop specific education programs in the future, with the NGO
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'Global Americanisation of Rulemaking?'.

assistant: **Objectives**

Emerging but extensive literature remarks the interdependence of governments also in administrative law. Transparency, openness, accountability, and the use of scientific evidence are values now common in rulemaking of many countries. Although theoretical and empirical efforts to frame and evidence the Americanisation of administrative law and regulatory governance, a metrical measure for assessing the extent of convergence is still lacking. This research project fills this gap, by assessing the extent of converge among OECD and EU member states. An index of similarity over four decades is derived from an original data set on administrative requirements. This index allows to measure the extent of similarity across four decades and test alternative convergence explanations, by relying on different methods.This research aims to assess the explanatory power of the underlying causes of Americanisation against the emergence of a European Administrative space via harmonisation, the transnational communication facilitated by the OECD, as well as globalising economic and political structures. The proposed research attempts to make the following three contributions to the literature of political science and comparative administrative law. The first contribution concerns the concept formation of administrative rulemaking. The emergence and the evolution of regulatory governance is taken into account, in order to trace the sequence and the cross-national pattern of regulatory oversight adoption. The second contribution refers to the literature of ‘global administrative law’, by assessing and estimating the extent of rulemaking process convergence. The final contribution is to the methodological literature on policy convergence.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Valorizing Andean microbial diversity through sustainable intensification of potato-based farming systems' in the context of a Seventh Framework call.

assistant: **Objectives**

VALORAM aims at exploring and valorizing Andean soil microbial diversity for the development of alternative, efficient technologies and crop management practices to improve the sustainability and productivity of Andean cropping systems benefiting rural farming households. The project will focus on potato because of its global importance for small-scale farmers in the central Andean highlands. The participants will use metagenomic, genomic, proteomic and metabolomic analysis to identify novel traits of microorganisms and characterize beneficial soil microbial communities, to achieve the objective. The project specific aims are to (1) explore the agro-ecosystem functions of soil microbes in potato-based cropping systems and preserve the components of this microflora in international culture collections, (2) elucidate the role of rhizosphere microorganisms and communities in promoting plant growth, suppressing soil borne disease and priming plant biotic defenses, developing eco-efficient technologies/products for sustainable crop production systems, (3) develop applied technologies and knowledge-based systems to improve the sustainability and resilience of potato based cropping systems for the benefit of the indigenous farmers and (4) promote the exchange of scientific knowledge and technologies among partners and the LA scientific community to impulse research in this area and support the continuous development of crop production technologies. The strategy for VALORAM implementation is to engage LA and EU partners in developing and further stengthening collaborative research activities in order to sustainably improve potato-based systems. This is supported by a multidisciplinary team of experts with highly complementary skills and based on a robust management structure wi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'SST Networking, Security & Data sharing. Horizon Europe TOP5' (2021-2027).

assistant: **Innovation**

This Horizon Europe grant is to be understood within a wider context on EUSST, where other grants are being executed simultaneously with the common objective of improving the EU autonomy on Space Surveillance and Tracking.In particular, this TOP focuses on:- Support the upgrade, development and security issues of EUSST infrastructure based on the European network of assets (sensors, operation centres, front desk …)- The EUSST infrastructure will remain under national control (meaning mainly sensors and operation centres), but increased coordination is needed due to the increased number of assets contributing to the European SST system. - SST networking of sensors & operation centres (EU SST network Command & Control): considering the increased number of objects to be handled, an increased number of events and users is expected.- The European SST system has to evolve to a coordinated scheduling of the resources and assets, ensuring that the events are covered in an optimum way, while the current survey and tracking of the space objects population continues to be performed.- Research on EUSST network hardening against external threats: the research concerns security-critical aspects of the existing EU SST network. - Next generation exchange protocols / solutions for SSA enhancing interoperability and security (robustness, information assurance, intrusion detection…)The starting point of these activities is the output of 2-3SST2018-20, which constitutes the state of the art on SST matters.


**Keywords:** EUSST, SST, Partnership, Front Desk, Services, Assets, Sensors, Radars, Telescopes, Lasers, Catalogue, Users, Collision Avoidance, Re-entry, Fragmentation, Survey, Tracking, Autonomy
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'Rome's Mediterranean Ports'.

assistant: **Objectives**

Rome was connected to its Mediterranean provinces by commercial routes articulated through networks of ports acting as poly-functional nodes. Ships, people and goods moved along these, drawing the micro-regions of the Mediterranean into closer economic and commercial relationships with the City. Central to the success of these networks were the major ports through which were channelled major commercial flows moving between Rome and its maritime hub at Portus, and its Mediterranean provinces, and their relationships to lesser regional and anchorages. All of them can be described in terms of loosely configured “port-systems” that ensured the movement of ships and their cargoes around the Mediterranean. Some of these, particularly in the eastern Mediterranean, can be traced back to the Hellenistic or earlier periods. ROMP will address specific questions relating to the capacities of and inter-connections between a range of 30 ports in ways that will allow us to better understand their role in helping ensure the cohesion and integrity of the Roman Mediterranean during the imperial era. These concern (1) the layout of Roman ports, (2) the organization of commercial activity focused at them, (3) hierarchies of ports, and (4) pan-Mediterranean commercial and social connections between ports. In addressing them, the project will apply suites of existing techniques in archaeology, ancient history and palaeo-environmental studies to a range of ports. It is an approach that builds upon the PI's belief in the value of integrating archaeological techniques and historical approaches to the study of the past, and the interpretation of individual port sites within a broader Mediterranean context. In so doing, the project moves beyond the state of the art in port studies, and raises iss