RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Fourth Framework proposal on 'Catalytic and structural characterisation of new high silica microporous materials'.

assistant: **Innovation**

Research objectives and content Zeolites and related microporous solids are well established as versatile materials readily adaptable to suit many important processes and applications. As part of an on going synthetic program at the host institution, a new series of high silica microporous materials have been produced which further extends the range of existing materials. The project proposed here is concerned with the catalytic and structural characterisation of these new microporous structures. From a combination of catalysis studies on the aluminosilicate variants of these materials, together with the structure determination of the framework of these solids a deeper understanding of the shape selectivity-reactivity relationship, a cornerstone of zeolite science, will be gained. It is also proposed to examine the structure of the as-prepared materials, to determine the role of the structure-directing organic molecules which template the formation of these solids. This will complement the chracterisation of these new solids and provide valuable case studies for computational modeling of these materials. Training content (objective, benefit and expected impact) The proposed project offers the chance to develop existing skills and to learn new aspects and techniques in solid state chemistry. The catalysis part of the research will allow experience to be gained in the field of industrial and applications orientated chemistry.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Unbiased niche identification and manipulation in stem cells and cancer' (EnviroTag).

assistant: **Concept**

For the longest time, researchers assumed that stemness is a cell-intrinsic feature. However, recently my work and the work of others have shown that specific microenvironments can restore stem cell identity in differentiated epithelial cells or induce transitions from one mature cell type to another. This instructive capacity of niches is equally important in homeostasis and disease. Like stem cells, cancer cells only survive in particular environments that nurture and protect them. This is particularly evident in the process of metastasis where only 1 out of 10 000 circulating tumor cells will find a suitable niche to grow. Nevertheless, our understanding of stem cell and cancer niches is still very limited. The main reason for this knowledge gap is the lack of suitable analysis tools.In the course of the EnviroTag project my team and I will generate a novel type of in vivo reporter system, which can label and genetically engineer the cellular environment of any cell of interest. We will use a multidisciplinary approach that combines the EnviroTag system with single cell sequencing, 3D-reconstructed confocal microscopy and organoid technology to study the dynamic roles of niche composition during tissue regeneration and cancer progression. Focusing on the gastro-intestinal tract, we will investigate how changes in the stem or cancer cell microenvironment control success and failure during tissue repair and the earliest steps of metastasis. Thereby, we will generate a new understanding of microenvironmental dynamics and uncover new regulatory mechanisms, signals and cell types that can be targeted to stimulate tissue regeneration or prevent metastatic spread.Objectives:1) Establish a spatial in vivo reporter system for microenvironmental labelling and manipulation 2) M
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon 2020 proposal for the project 'Establishing the dual role of serotonergic neurons of the dorsal raphe in flexible behaviors'.

assistant: **Objectives**

One the most relevant neuromodulator which affect the organisms’ ability to adapt to environmental changes and to aversive events is serotonin or 5-hydroxytryptamine (5-HT). 5-HT has long and widely been implicated in the pathophysiology of several psychiatric diseases such as obsessive-compulsive disorder, anxiety, drug addiction and depression. Emerging evidence indicate that 5-HT also plays a crucial role in cognitive and behavioral flexibility. Serotonin neurons respond to aversive stimuli and promote shifting behavior away from aversive events or frustrative non-reward but also respond to reward and prevent shifting behavior in the face of effort or delay. How 5-HT neurons from the dorsal raphe (DRN5-HT) both promote and prevent shifting behavior is, however, still unclear. Addressing this question requires a better understanding of the role of the 5-HT systems in controlling flexible behavior. Using calcium imaging, optogenetic manipulation, high density fiber photometry and closed-loop brain manipulation, in a comprehensive procedure assessing behavioral flexibility, FLEX5 aims to define the behavioral events that activate DRN5-HT neurons and define the role of 5-HT in its network.


**Keywords:** Serotonin, behavioral flexibility, dorsal raphe
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'High-pressure phase transitions inpolyamorphic systems of semiconducting elements' (2002-2006) targeting Sixth Framework.

assistant: **Abstract**

The project is aimed to acquisition the experimental information about the transformations occurred in amorphous silicon and germanium within wide pressure and temperature intervals, characterization of the different amorphous phases formed under high pres sure.This will be done by in situ high-pressure experiments using Raman, infrared spectroscopy and X-ray diffraction. The incoming phase period is based in the University College of London (UCL) using the range of diamond anvils cells installed there for optical measurements. The structural experiments are planned on the X-ray synchrotron sources in SRS (Daresbury, UK) and ESRF (Grenoble, France).The return phase in the Institute of Solid State Physics (Russian Academy of Sciences), is planned to obtain bulk samples of the quenched high-pressure amorphous Si and Ge samples by means of 'quenching under pressure' method employing large-volume high pressure cells and for measuring the pressure dependence of the superconducting transition temperature of Si and Ge samples up to 60 GPa.The project is a stage of the general research program planned UCL and is directed to complex investigations of the 'polyamorphism' phenomenon in the systems demonstrated the first-order phase transitions within liquid (amorphous) state. The experience, experimental skills and technical approaches obtained during the accomplishment of the project will be used for studding ionic-covalent framework liquids such as SiO2 and GeO2 important from the geological point of view.The project can be accomplished completely in the experimental centres of Europe serving for promotion of the intra-European scientific relations. The engaging of the Russian scientists for realization of the research projects in Europe will also work for these very objectives
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon Europe proposal on 'Losing genes but gaining symbionts: Why beetles outsource key metabolic traits'.

assistant: **Concept**

Herbivorous beetles represent one of the most successful animal radiations. The cooption of microbial enzymes is cited as a key innovation that endowed beetles with the catalytic tools to breach the plant cell wall and facilitated the evolution of remarkably diverse herbivorous habits. Beetles incorporated microbial plant cell wall-degrading enzymes either through horizontal gene transfer or symbiosis. This binary distribution is most evident in leaf beetles (Coleoptera: Chrysomelidae), where the loss of horizontally acquired pectinases coincided with the evolution of functionally convergent digestive symbioses. Prior efforts either examined the role of horizontal gene transfer or symbiosis in shaping the phenotypic complexity of herbivorous beetles. But a comprehensive approach reconciling both fields is lacking. SYMBIVORE aims to fill this gap by defining the metabolic requirements and determining the physiological and ecological conditions favoring symbiosis as an alternative strategy for beetles to deconstruct pectin. The proposed research is integrative and extends beyond the state-of-the-art by examining the adaptive importance of symbiosis relative to horizontal gene transfer across multiple scales of biological organization. From the molecular and genetic mechanisms underlying symbiont acquisition, to the biochemical, physiological, and ecological consequences of outsourcing a key metabolic trait. Through comparative genomics, transcriptomics, structural biochemistry, bioassays, and nutritional geometry, SYMBIVORE will address the hypothesis that symbiosis upgraded the digestive physiology in a subset of beetle clades, thereby relaxing selection for these insects to endogenously maintain essential genes. SYMBIVORE capitalizes on the functional overlap conferred
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Leading Edge Box Design for Swept Flow Control Wing' (LEBOX) under Seventh Framework.

assistant: **Objectives**

The subject of the proposed project LEBox will be design of an integrated leading edge (L/E)-Box with pneumatic flow control devices for mid-scale wind tunnel testing on a suitable swept constant chord (2.5D) model. Dynamic vortex generator jets (VGJs) are chosen as the most suitable active device to control turbulent flow separation at the leading edge. The geometry of these devices in particular with respect to a swept integration will be evaluated to find important parameters such as the optimum skew angle, optimum position at the leading edge and optimum size, e.g. relative to the boundary layer. This will be done by means of comprehensive numerical simulations. The actuator geometry will be designed for the proposed (new) L/E-Box as well as for an already existing actuator system on the shroud (if a specific wind tunnel model will be used). A new L/E-Box for a swept mid-scale wind tunnel model will be designed to the point of production drawings, manufacturing (preferably by an external partner from the SFWA /WP114 consortium) will be supported. The device will be integrated with the sensor and actuator systems and tested in a mid-scale facility. For these steps background knowledge will be taken from previous or current projects as well as from the results of the numerical evaluation and from previous wind tunnel tests. The results will be analysed with respect effectiveness, efficiency and to the preparation of large-scale wind tunnel tests.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Fifth Framework proposal on 'Orexins / hypocretins - new targets for therapeutic control of feeding and sleeping disorders (OREXINS)'.

assistant: **Innovation**

Since their discovery in 1998 two peptides named origins (synonym hypo cretins) receive much attention because of their central role in the regulation of basic body functions: food intake, energy balance and the sleep waking cycle. Loss of the origin containing neurons in the hypothalamus, which project widely in the brain, is the cause of human narcolepsy, a disabling disorder of vigilance, which also occurs in dogs where the G-protein coupled origin receptors are missing. Understanding the functions of this central command system on the molecular, cellular, network, behaviour and clinical levels is necessary for a rational therapeutic approach to disorders of feeding and sleeping. Our consortium representing molecular biology, physiology, pharmacology, biological psychology, clinical medicine and the pharmaceutical industry will take this task.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Exploration below the tip of the microtubule', explaining its relevance to EU policy priorities under Horizon 2020.

assistant: **Impact**

Microtubules (MTs) are dynamic cytoskeleton filaments. They permanently transit between growth and shrinkage. This famous “dynamic instability” is governed by the addition and loss of tubulin dimers at their tips. In contrast to the tip, the MT lattice was considered to be a passive structure supporting intracellular transport. However, we recently found that MT lattice is dynamic and active! Actually, tubulin dimers can be exchanged with the cytoplasmic pool along the entire length of the MT. These incorporations can repair sites on the lattice that have been mechanically damaged. These repair sites protect the MTs from depolymerisation and increase the MT’s life span. This discovery opens up a new vista for understanding MT biology.            First, we will investigate the biochemical consequences of MT-lattice turnover. We hypothesise that tubulin turnover affects the recruitment of MAPs, motors and tubulin-modifying enzymes. These recruitments may feedback on lattice turnover and further regulate MT life span and functions.            Second, we will investigate the mechanical impact of the MT-lattice plasticity. Tubulin removal is likely to be associated with a local reduction of MT stiffness that can impact MT shape and the propagation of forces along the lattice. We anticipate that such effects will require us to reformulate the biophysical rules directing network architecture.            To achieve this, we will use reconstituted MT networks in vitro to investigate the molecular mechanism regulating MT-lattice plasticity, and cultured cells to test the physiological relevance of these mechanisms. In both approaches, microfabricated devices will be used to control the spatial boundary conditions directing MT self-organisation.            By exploring the hidden
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon 2020 proposal on 'Smart Pickering Emulsions'.

assistant: **Concept**

The main objective of SPicEs is to prepare the ER for an independent career in the research sector, by training him within the framework of a high-quality research project at the interface between the science and engineering of materials, chemistry and physics. The scientific objective of SPicEs is the development of a general approach to organize nano-structured materials on the interfaces of liquids, as in Pickering Emulsions, to yield stimuli-responsive devices. The assembly of nanomaterials into components and devices is a requisite to develop marketable applications. Pickering emulsions are an emerging technology with great potential in this field, however many aspects of interest remain to be studied, such as the combination of different materials in a single supracolloidal structure. Within SPicEs, microfluidic methods will be used to generate droplets with a high degree of control, covering them with functional nanoparticles capable of imparting each droplet with useful properties such as the ability to respond to external stimuli (light, heat, pH changes). The emulsions prepared in this way will be used in proof-of-principle applications, as random lasing matrixes and carriers for the controlled delivery of chemical payloads.The project will provide the ER a possibility to train and put his abilities to test on an international level. For him, it will represent a strong starting point for an independent career in research. His training will encompass fundamental and applied science, as well as manuscript preparation, IPR issues, networking and soft skills such as leadership, communication and teamwork in a multi-ethnical, highly multidisciplinary group. For the host institution this project will represent a possibility to broaden the horizon of one of its most
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Chromatographic micro-column development for pharmaceutical applications in zebraFish' (ChromaFish) under Horizon 2020.

assistant: **Objectives**

ADME (Absorption, Distribution, Metabolism and Excretion) studies focus on the examination and prediction of drug behavior in the human body. Zebrafish present a good in vivo model for this purpose since they comply with the 3R principle of humane animal research (Replacement, Reduction and Refinement). The research group of Prof. Deirdre Cabooter recently developed a novel sample preparation and analytical method to accurately measure the whole-body uptake of drugs in zebrafish. The next step is to further exploit the developed methodology to study other aspects of ADME, in particular uptake and metabolisation of new drug candidates in the brain. Since the blood-brain barrier is a major obstacle preventing many pharmaceuticals from achieving effective concentrations in the brain, infinite small concentrations are to be expected. The detection of low brain concentrations can be improved by using advanced analytical techniques with lower sample dilution and thus enhanced detection limits. An important part of this research project will therefore be devoted to the development and characterization of new capillary column formats based on core-shell particles offering unprecedented sensitivity and unique selectivities. A new brain dissection technique will moreover be developed to isolate the brain from the circulating blood and investigate the uptake and metabolisation of selected pharmaceuticals in the brain. Molecular transport routes will be unraveled and the uptake in specific parts of the brain will be investigated as well using laser microdissection microscopy. Finally, the potential of the developed methodology to provide a robust protocol for brain uptake measurements in industrial applications will be investigated by comparing the outcome obtained by zebrafish exp
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon Europe proposal on 'The concept of teotl: a complex approach to the principal religious category of pre-Hispanic Central Mexico'.

assistant: **Innovation**

Since the 16th century, the polymorphous and overlapping identities of Nahua (Aztec) deities have puzzled, first, the friars carrying out their evangelization project in New Spain, and later, throngs of researchers eager to better understand the pre-Hispanic Nahua system of beliefs. Despite many scholarly efforts, today, we are still far from satisfactorily apprehending the various intricacies of of the concept of teotl. Rendering of the Nahuatl term teotl as god, helpful as it was in the process of cultural translation, neither reflects its full semantic scope nor explains ontological and cosmological implications of this notion. Nevertheless, up to this date, no one has attempted to embrace this problem bringing together multiple yet complementary perspectives integrated by an innovative set of analytical tools.The project TEOTL aims at unfolding the underlying notions of this pre-Hispanic Nahua key religious concept by combining its four crucial aspects. These are: 1. Nahua pantheon; 2. teotl in relation to other important religious categories, such as tonalli, nahualli, or ixiptla; 3. philological analysis of the term teotl in collocations, compounds, and the discourse of Nahuatl texts; 4. appropriation of the notion of teotl in colonial Nahuatl Christian discourse.Using the heterogeneous textual and iconographic dataset derived from the well-known source base, TEOTL will employ a novel combination of research methods at the intersection of traditional (ethnohistory, religious studies, art history, philology, linguistics) and digital humanities (network analysis in historical sciences). This unexplored research path will lead to a reconceptualization of our approach towards the Nahua notion of the sacred and its decolonization. Also, the obtained results may be appl