RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Hijacking cell signalling pathways with magnetic nanoactuators for remote-controlled stem cell therapies of neurodegenerative disorders' (MAGNEURON) under Horizon 2020.

assistant: **Objectives**

Neurodegenerative diseases, such as Parkinson’s disease, are a major public health issue given the aging population inEurope and beyond. While curative pharmacological treatment of these diseases is not in sight, cell replacement therapies(CTs) are considered very promising, in particular with the advent of stem-cell reprogramming technologies. However, afundamental challenge in the medical application of CTs in the brain of patients lies in the lack of control of cell behaviour atthe site of transplantation, and particularly their differentiation and oriented growth. The aim of this project is to introduce afundamentally new concept for remote control of cellular functions by means of magnetic manipulation. The technology isbased on magnetic nanoparticles functionalized with proteins involved in cellular signalling cascades. Thesebiofunctionalized MNPs (bMNPs) will be delivered into target cells, where they act as intracellular signalling platformsactivatable in a spatially and temporally controlled manner by external magnetic fields. The project will focus on engineeringthese tools for the control of neuronal cell programming and fibre outgrowth by hijacking Wnt and neurotrophin signalling,respectively, with the ulti-mate objective of advancing cell replacement therapies for PD using dopaminergic precursorneurons.To achieve this ambitious goal, we have gathered an interdisciplinary consortium interfacing scientists having cutting-edgeknow-how in bMNP engineering, surface functionalization and cellular nanobiophysics with renowned experts in neuronalcell differentiation, stem-cell reprogramming and regenerative (nano-)medicine. By exploiting this complementary expertise,a novel, versatile technology for magnetic control of intracellular signalling is envis-aged, which
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'The molecular basis for the ability of PLC zeta to stimulate mammalian embryo development' to be submitted under Seventh Framework.

assistant: **Abstract**

Oocyte activation is the earliest step of embryonic development following mammalian fertilization and is triggered by a characteristic series of cytoplasmic Ca2+ transients, known as Ca2+ oscillations. Over the last decade, growing evidence indicates that the physiological agent responsible for the generation of Ca2+ oscillations and subsequent oocyte activation is a sperm-specific isoform of phospholipase C (PLC), PLC-zeta (PLCz). The sperm-borne PLCz stimulates Ca2+ oscillations in eggs after sperm-egg membrane fusion by promoting Ca2+ release via activation of the inositol 1,4,5-trisphosphate (IP3) signaling pathway. PLCz is the smallest mammalian PLC comprising a tandem pair of EF hand domains, the catalytic X & Y domains and a C2 domain. PLCz hydrolyses the membrane lipid, phosphatidylinositol 4,5- bisphosphate (PIP2), to liberate IP3 which triggers calcium release from the endoplasmic reticulum. The fundamental role of PLCz in mammalian fertilization has been further highlighted by recent clinical studies that have directly linked abnormal expression profiles of PLCz with documented cases of male infertility. This fellowship will establish the molecular mechanism of fertilization in mammals by demonstrating whether PLCz is the sole sperm factor for the generation of Ca2+ oscillations. I will identify distinct intracellular egg proteins that are involved in interaction and targeting to discrete subcellular compartments a process that enables precise modulation of sperm PLCz activity. I will determine how PLCz enzymatic activity is regulated by the binding of the egg protein(s) and to which of the PLCz domains the binding occurs. Gaining an insight on the regulatory mechanism of PLCz action within the egg will facilitate the potential application of PLCz as a therap
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Designing Conjugated Polymers for Photocatalysis and Ion Transport', explaining its relevance to EU policy priorities under Horizon 2020.

assistant: **Impact**

Solar energy conversion will play an essential role in the future supply of clean energy. Secure access to energy sources will require energy conversion technologies that are low impact, distributed and accessible both technically and financially. Molecular electronic materials embody these possibilities, offering facile synthesis, low energy production and the versatility to allow performance to be maximized for specific applications. Moreover, they bring appealing similarities with nature’s intrinsically low impact energy conversion materials. Whilst molecular semiconductors have been studied in detail for solar-to-electric energy conversion they have seldom been studied for solar-to-chemical conversion or for charge storage. However, they bring exciting potential advantages in terms of their light harvesting properties, the range of microstructures possible and the ability to tune their electrical properties. Polymer materials applied to solar chemical generation could open up an innovative route to artificial fuels, with the option to control light harvesting and charge separation through structural control. Polymer materials applied to mixed (electronic / ionic) conduction provide a route to lower cost electrochemical storage, as well as to biocompatible devices and sensors. Stimulated by recent experimental breakthroughs in the application of polymers as photocatalysts and ion transport media I will exploit my expertise in multi-scale modelling and functional characterization of molecular electronic materials and devices to develop a design framework for energy conversion and storage in conjugated polymer materials. This proposal aims to disentangle the parameters that govern the performance of conjugated polymer based photocatalysts and ion transport media to dis
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Determination of the structure of the disease-associated, srapie form of the prion protein' (2002-2006) under Sixth Framework.

assistant: **Objectives**

The proposed work seeks to determine the tertiary structure of the scrappy form of the prion protein. Prion diseases are marked by the conformational transition of the prion protein (PrP) from its cellular, dominantly alfa-helical form (PrPC) to a beta-she et-containing, protease-resistant form (PrPSc). Since PrPSc is insoluble and forms aggregates, most structure-determination methods are unfeasible. A new approach is proposed here for mapping the secondary structure of PrPSc, using mutant analogues of PrP, in which amino acids are systematically replaced by alfa-aminoisobutyric acid (Aib), an unnatural amino acid that cannot adopt backbone dihedral angles in the beta-sheet region of the Ramachandran map.These analogues can only be converted to the protease-resistant scrapie conformation when the residue replaced by Aib is in an alfa-helix (and not in a beta-strand) in the scrappy form. The tertiary structure of PrPSc than will be determined by computer modelling based on the experimentally determined secondary structure. This model will be validated experimentally by covalent cross-linking the appropriate segments of PrPSc. This structural information will facilitate the development of sensitive diagnostic procedure for early detection and effective treatment of the prion disease.I have conducted research for 6 years at Cornell University (US) and obtained expertise in protein folding and semi-synthesis as well as on the prion field. I would like now to return to Europe and capitalize my expertise in pursuing a carrier on conformational diseases as a principal investigator in a world-class European research institute.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Clinical Validation of an AI-based approach to improve the shared decision-making process and outcomes in Breast Cancer Patients proposed for Locoregional treatment' to be submitted under Horizon Europe.

assistant: **Abstract**

Breast cancer is the most commonly diagnosed cancer, with an estimated  2.3 million new cases per year globally. Approximately 90% of these patients will undergo breast surgery with/without radiation (locoregional treatment). Different surgical techniques can be offered to the patient, each leading to completely different aesthetic outcomes. Moreover, for different patients, undergoing the same surgery, the aesthetic outcome could be completely different based on individual patient's factors (e.g, age, body habitus). In the CINDERELLA project, we will be using the BreLO AI system  (an artificial intelligence-based tool for classification of aesthetic outcomes and matching data and photographs) integrated in CANKADO (a cloud-based healthcare platform) to create an easy-to-use application that can be used on any electronic device, to simulate visually to the patient the aesthetic outcome of a certain surgery. The CINDERELLA APProach is unique as it uses a clinical database and performs biometric matching, to create images that simulate to the patient the outcome of surgery. In the CINDERELLA trial (running during the project) we plan to compare if the application helped to fulfil the expectation and lead to a better quality of life. By feeding new clinical and photographic information to the CINDERELLA APProach after surgery, the artificial intelligence platform will be able to improve for the next cases.  This trial will be practice-changing in the field of patient education and shared decision making. CINDERELLA platform will help to overcome any miscommunication and potential boundaries in patient's or physician's understanding of the potential outcomes of locoregional treatment of breast cancer. We believe that the CINDERELLA APProach will be used in the future to oth
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Representation of space in the primate hippocampus' (1994-1998) targeting Fourth Framework.

assistant: **Abstract**

In recent years the idea that the hippocampal formation is crucial for the formation of some types of memory has been established. Within the scope of the role of hippocampus in spatial memory, many experiments have been developed on freely moving rats. For the first time, an experimental set-up allowing single cell recording in a freely moving monkey has been developed. Monkeys are useful in providing a model closer to the human hippocampus than rats. In this situation, in one monkey, no place" cells have so far been found. Instead, we have found a considerable population of "view" cells. These hippocampal neurons respond to a view of space 'out there', not to the place where the monkey is. This is helpful in understanding hippocampal function in humans. The grant would allow me to receive full training in the sophisticated techniques used and to extend the results to simultaneously recorded single neurons."
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Nett-forging with die-surface excitation' (DISURFEX) under Fourth Framework.

assistant: **Objectives**

Objectives and content The manufacture of engineering components for the aerospace, automotive and consumer-goods industries is often initiated by forging; increasingly, the demand is for the primary-conversion of raw material into a forging which replicates the nett-form of the component. The forging of components to close tolerances for these industries has been achieved at substantially higher cost as these have to be produced with dies which are manufactured from high-performance steels, with pre-stressing and surface treatments. The need for these additional features and treatments arises from the need to contain the higher abrasive stresses in the die and, consequently, higher forging forces which are encountered when nett-forging of engineering components.Solution: An alternative approach, to the addition of the special features, would be to reduce the die-surface stresses in critical sections of the die; this can be achieved by the application of die-surface excitation. A reduction of forging forces by 40% has been demonstrated by the University of Strathclyde; this would result in a corresponding increase in the life of nett-forging dies by, at least an order, thus establishing a more viable alternative to the current approach. Proposal: Research will be conducted, initially, to define a system for the simulation of excitation, which would enable rapid assessment of the geometrical aspects of die-design for surface excitation. Further an approach will be developed to support the economic evaluation of die-surface excitation; this will rely on FE techniques which will be initialised using friction data from Physical Modelling experiments. Both the above assessments would have to rely on clear definitions of the technology of die-surface excitation; this will be
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Proposal of Consortium AURASME4 for the support services enhancing the innovation management capacity of SMEs within the Enterprise Europe Network Auvergne-Rhone-Alpes (EURORAA4)' (EURORAA4).

assistant: **Innovation**

AURASME4 project is a subset of COSME EURORAA Consortium, based in Auvergne-Rhone-Alpes region France. Partners involved are Partner 1 (coordinator) and Partner 2. Partner 3 was not involved in this activity and is inactive since 2015 (amendment) and Partner 4 merged with Partner 1 (UTRO). The general objective of this proposal is to bridge the gap of lacking innovation management capacities within international level. The work program is stricly focused on the objectives described in the call for proposal on the implementation of the two required service package : Key Account Management for beneficiaries of the SME bénéficiaires of the H2020 European Innovation Council (EIC) pilot and Enhancing Innovation Management Capacities. The work programme describes the implementation of thes two packages'services, the detailed methodology and process for both service and their expected impact. A close link is ensured with the present EEN COSME related work program.


**Keywords:** Enhancing innovation management,  Key Account Manager,  SME Instrument ,  SMEs beneficiaries of the EIC Pilot,  Fast Track to Innovation ,  Future and Emerging Technologies Open
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Clone-based full-length RNA-seq for early diagnosis of cancer' (CancerFLCloneSeq), addressing dissemination and exploitation.

assistant: **Impact**

Intra-tumor genetic heterogeneity imposes a great challenge on cancer therapy. Resistance to molecularly targeted therapies and chemotherapy can arise from selective growth of pre-existing sub-clones that carries drug-resistance mutations, altered metabolic and/or epigenomic states, providing a survival advantages. Early detection of these subclonal states can thus significantly aid cancer therapy. However, attempts to profile various types of primary cancer cells using single-cell techniques are relatively poor. One of the major limitations is the significant dropout rate (i.e., loss of alleles) observed in single-cell RNA-seq. It severely affects our ability to leverage single cell RNA-seq to accurately profile somatic mutation, to reveal cancer driver mutation and even extract low/mid-level expressed genes and splicing. For that reason, most of the efforts to expose mutations that are critical for cancer growth and can subsequently lead to more effective treatment are based on the sequencing of bulk populations. However, due to the noise introduced by PCR, sequencing and alignment processes, bulk sequencing is limited to identify mutations with a frequency higher than 5%. Here we propose to develop a novel 3D clone-based full-length RNA-seq profiling technology. A preliminary version of this technology for digital profiling of mRNA, already allowed us to significantly improve sensitivity comparing to gold-standard single-cell RNA-seq methods. Using this preliminary version on clones of lung adenocarcinoma, we revealed novel cancer stem like subpopulation that could not be detected using regular single cell RNA-seq maps. Altogether, improving the ability to detect rare mutations (<5%), splicing events and transcriptional variation between cancer cells, will be an extr
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Dedicated CT of the Female Breast:  Feasibility, optimization and comparison to standard x-ray procedures (digital mammography and tomosynthesis)' (2007-2013).

assistant: **Innovation**

Early and accurate diagnosis of breast cancer in women is a pending challenge. Digital x-ray mammography is considered today’s state of the art in diagnosis although severe insufficiencies are acknowledged. Conventional film-screen mammography, digital tomosynthesis, ultrasound, nuclear medicine and magnetic resonance imaging are also in use or under investigation. There is a general consensus that 3D imaging could offer significant advantages.  Dedicated x-ray computed tomography (CT) of the female breast without exposure of the body trunk appears to be a further candidate with improved diagnostic capabilities, but radiation protection issues have to be solved. In consequence, this project focuses on the development of a dedicated scanner using novel technology and optimisation strategies. We intend to prove the feasibility of CT of the breast with very high spatial resolution of 100 µm or better and high soft-tissue differentiation at dose levels equal to or below those of two-view digital mammography. First simulation studies support the concept.  To allow for balanced conclusions on justification of practice and risk assessment, the performance of breast CT will be validated and compared against digital mammography and tomosynthesis primarily, but also against any other novel approach. To achieve the proposed goals three European scientific institutes of international reputation and two manufactures with the respective know-how have formed a consortium. The necessary equipment, tools, test and measurement approaches are available; new multi-modality tests have to be developed for a meaningful comparison of 2D and 3D imaging. It is our intention to provide proofs of concept and results on breast CT in the relatively short time span of 30 months. Pending on the result