RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'FISCI: A new bio-index for the assessment of stress condition in acultured marine fish.', explaining its relevance to EU policy priorities under Fourth Framework.

assistant: **Impact**

The overall objective of the project is to develop a non-invasive method for detecting general and specific stressor-related stress effects in fish reared in aquaculture.Within the general research framework the project aims at two interrelated objectives:To detect, analyse and quantify stress effects, under both laboratory and fish farm conditions, in two Mediterranean marine fish species, gilthead seabream (Sparus auratus) and European sea bass (Dicentrarchus labrax), which are of major economic importance for European aquaculture, using as parameters changes in the skin and gill epithelia, and the mucus layer covering the skin: (a) changes in tissue morphology (b) enzymatic activity in skin and skin mucus (c) concentrations of the stress hormone cortisol in blood serum and skin mucus and (d) non-enzymatic biochemical parameters in skin mucus samples. The stressors to be applied (low dissolved oxygen levels, high ammonia levels, and handling stress) are relevant to aquaculture.To select subsequently the analytical methods and stress parameters that will prove to be reliable and easy to perform, using a sampling procedure from which the sampled fish can easily recover. The selected methods will be combined as bio-indicators through the establishment of a new stress-index, the Fish Stress Condition Index " (FISCI), that will enable the characterisation of stress condition in cultured fish and will reflect both fish health and water quality This will provide fish farmers with a protocol method to check the stress condition of their fish."
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Biological Surfaces and Interfaces' will create measurable scientific, societal and economic impact aligned with Fifth Framework.

assistant: **Impact**

The main aim of this conference series is to provide a high level introduction to an important and developing multidisciplinary research field. The conference series is directly aimed at enhancing the understanding of biologically important interfaces through the application of surface science and as such is expected to open dialogue and lead to collaboration between physical scientists, biologists and clinicians. This is a rapidly growing field, which is significantly under-represented within the European scientific community. While traditional" surface science is already represented by a EURESCO conference series under the umbrella of the European Science Foundation (Fundamental Aspects of Surface Science) the scope of the planned series is well beyond the fundamental nature of the existing series. Surfaces within biological systems are extremely complex, stretching the capabilities of surface analytical techniques and presenting novel technical and scientific challenges. We feel it is important that young European researchers are ready to meet these challenges and we believe that a dedicated conference series will provide an excellent forum to drive forward this exciting interdisciplinary field.Two meetings are outlined within the current proposal. Both will include key-note lectures to provide an overview of the field in its current state, as well as specific "foresight" topics which it is anticipated will make a strong impact in the near future. The first meeting in the series will introduce the complexity of biological interfaces and focus on the impact of "traditional" and novel surface science techniques within this field. Both surface analysis and surface modification aspects will be addressed.The second meeting has been designed with a more applied nature. The
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Mechanisms underlying functional disabilities in chronic musculoskeletal pain: the role of protective factors' (PROTECT).

assistant: **Innovation**

Chronic musculoskeletal pain is a major health issue, which translates into a severe economic burden. Studies on chronic pain conditions have focused primarily on characterizing pain mechanisms with a focus on alleviating clinical pain intensity. The success of these studies in terms of effective treatment remains extremely limited. Rather than focusing on pain intensity, PROTECT will focus on functional disabilities associated with chronic musculoskeletal pain, which are the main complain of patients. They can be extremely debilitating and often result in social isolation of persons with chronic musculoskeletal pain. To date, their mechanisms remain poorly understood. A better understanding of these mechanisms is essential to improve treatment efficiency. In work package (WP) 1 of PROTECT, the impact of athletic identity and mental and physical fatigue on the relationship between resilience and functional disabilities will be studied in adults and minors with chronic musculoskeletal pain (RO1). Resilience is associated with functional disabilities, athletic identity and fatigue, a frequent comorbidity of chronic musculoskeletal pain. Characterizing the complex relationship between these factors using computational modeling will define new treatment targets that can be addressed by adapting already available therapeutical interventions. All factors will be assessed using questionnaires, as well as physical and behavioral tasks. In WP2, resting-state fMRI will be used to assess brain networks of functional disabilities in adults with chronic musculoskeletal pain. In particular, the role of the Default Mode Network (DMN) in functional disabilities will be investigated (RO2). Changes in DMN connectivity have been associated with acute and chronic pain, as well as with resi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Establishment of a FramewORk for Transforming current EPES into a more resilient, reliable and secure system all over its value chain' in the context of a Horizon Europe call.

assistant: **Objectives**

Prompted by the need to comply with environmental and societal concerns, Electrical Power and Energy Systems are undergoing an unprecedented transformation, demanding urgent upgrades to make them more reliable, resilient and secure. Modernization of current grids will greatly reduce the frequency and duration of power blackouts, diminish the impact of disruptive events and restore service faster when outages occur, creating broad benefits to society and economy. eFORT approach will enable the further upgrading of the energy grid without affecting the security of supply and increasing their reliability and resiliency against extreme weather events, man-made hazards and equipment failures. eFORT addresses this complex challenge by gathering a consortium of 24 partners, from 10 EU countries, that provides the needed expertise. The project will put in place a set of solutions at the cyber and physical layers for detecting, preventing and mitigating vulnerabilities and threats. Among them, an interoperable Intelligent Platform will set a common foundation for grid characterization and vulnerability overseeing, as well as gather information from smart grid components and apply heavy-duty algorithms, whereas Asset Management developments will strengthen grid infrastructure robustness, which will be empowered by the addressed Digital Technologies. All these elements will be validated in relevant environments coming from 4 demo cases covering the whole grid value chain: (i) a transmission network (The Netherlands); (ii) a remote distribution grid (Italy); (iii) a digital substation in Ukraine; and (iv) a micro-grid in Spain. Moreover, eFORT relies on several horizontal actions aiming at empowering EPES players by establishing a common regulatory and standardisation framework, pe
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Elucidating inner cells contribution to pavement cell shape acquisition in Arabidopsis' in the context of a Horizon Europe call.

assistant: **Objectives**

In plants, the outermost epidermal cell layer is critical for protecting the entire plant against external threats such as rain, wind and pathogens. The plant epidermis is a complex tissue consisting of multiple cell types, all with different shapes, functions and developmental trajectories. Although studied in isolation, our understanding of how these cells interact to ensure tissue integrity is poor, but at present, the outermost jigsaw-puzzle-shaped pavement cells have emerged as key players. Recent findings in the host lab suggest that the diverse physical and/or mechanochemical nature of the cell wall is crucial for pavement cell-shape acquisition, and thus under influence of the underlying tissue layers. However, yet the identity of the putative signals derived from inner cell layers, defining the shape of epidermal cells and the integrity of the multilayered leaf tissue, remains to be explored. Hence, in iCON I will join the Robert (host) lab to disentangle the signaling networks and mechanical constraints of inner mesophyll cells that determine epidermal pavement cell shape and organogenesis of the leaf, using a suite of novel and multidisciplinary approaches in the model plant Arabidopsis thaliana. Specifically, I will acquire new skills in cutting-edge microscopy, cell biology and biomechanics to generate a high-resolution 3D map and uncover mechanical/physical cues, and leverage my expertise in transcriptomics, genetics and molecular biology to determine molecular components, together allowing me to decipher the overall mechanism via which mesophyll cells orchestrate pavement cell shape acquisition. Besides contributing new knowledge, iCON will allow me to expand my scientific expertise and gain key transferrable skills, boosting my strive to become a researc
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon Europe proposal on 'Creation and promotion of Forest and Agriculture Networks to boost Bio-Based Technologies adoption and Value Chain development'.

assistant: **Innovation**

In response to the increasing need for grassroots initiatives and knowledge sharing to address major challenges such as climate resilience and increased mitigation of GHG emissions, while supporting zero waste and circular economy with biomass reuse, BBioNets will constitute a thematic network that will rely on, promote, and further advance the work carried out by EIP AGRI Operational Groups (OGs) with respect to management and/or processing of agricultural and forest biomass with Bio-Based Technologies (BBTs). Applying the quintuple helix model and a multi-actor approach both within the consortium itself and on the ground activities, BBioNets will set up 6 regional Forest and Agriculture Networks - FANs (IE, ES, IT, GR, PL, CZ) that will ensure balanced representation of all AKIS stakeholders. FANs will identify local needs and, through co-creation and joint actions, prioritise specific BBTs, benefit from and validate knowledge material developed by the consortium building on previous OG and EU-funded project results. They will assist BBioNets in sharing BBT knowledge ready for practice to farmers and foresters, boosting the (re)definition of value chains, stimulating cross-fertilisation beyond borders, and bringing Europe to the forefront of farming, forestry, and bioeconomy with economically viable and sustainable practices. BBioNets’s solid foundation and strength lies in its partners’ a) wide networks covering the Agriculture, Forestry and Bioeconomy world, b) long-established collaborations with OGs, c) extensive knowledge of bio-based technologies and solutions and d) long experience in awareness raising, knowledge transfer, capacity building, networking and value chain creation services.


**Keywords:** bioeconomy, bio-based technologies, education, training, fa
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Seventh Framework proposal on 'Combatting Bacterial Resistance in Europe - Carbapenem Resistance'.

assistant: **Concept**

Multi-drug resistant Gram-negative bacteria (MDR-GNB): the ultimate challenge!Antibiotic resistance is a global public health concern recently elevated to the top three threats identified by the WHO, and subject of numerous national and international government activities. Although focused strategies have beneficially influenced infection rates due to methicillin resistant Staphylococcus aureus (MRSA), infections due to MDR-GNB are rapidly increasing. In a very recent report the Centers for Disease Control and Prevention concluded that Carbapenem-Resistant Enterobacteriaceae (CRE) represent an immediate public health threat that requires urgent and aggressive action (CDC: Antibiotic Resistance Threats in the United States 2013).The epidemiology of MDR-GNB, and especially of CRE, is characterized by large heterogeneity in genotypes (with >20 reported resistance genes, such as NDM, KPC, VIM, OXA-48) and even larger heterogeneity of phenotypes, seriously hampering rapid detection of resistant types when using routine susceptibility testing. Treatment of infections caused by CRE relies on combinations of “older” antibiotics, lacking a strong evidence base of effectiveness and safety. New treatments for these infections are, therefore, urgently needed.The COMBACTE-CARE consortium is building on the recently funded Topic 1a/b/c consortium: COMBACTE, and contains three networks: CLIN-Net, LAB-Net and STAT-Net. To fulfil the specific objectives of Topic 5 the consortium has focussed on capacity building in clinical sites in some of the new member states in south-eastern Europe, where infection rates with MDR-GNB are expected to be high. Together with high-quality study sites in countries with endemic levels of MDR-GNB (such as Israel and Greece) CLIN-Net and LAB-Net will maximi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'The Initiation of Eukaryotic DNA Replication: Mechanism, Regulation and Role in Genome Stability' under Seventh Framework.

assistant: **Concept**

In each cell cycle, eukaryotic cells must faithfully replicate large genomes in a relatively short time. This is accomplished by initiating DNA replication from many replication origins distributed along chromosomes. Ensuring that each origin is efficiently activated once and only once per cell cycle is crucial for maintaining the integrity of the genome. Recent evidence indicates that defects in the regulation of origin firing may be important contributors to genome instability in cancer. Strict once per cell cycle DNA replication is achieved by a two-step mechanism. DNA replication origins are first licensed by loading an inactive DNA helicase (Mcm2-7) into pre-replicative complexes (pre-RCs). This can only occur during G1 phase. Initiation then occurs during S phase, triggered by cyclin dependent kinases (CDKs) and Dbf4-dependent kinase (DDK), which promote recruitment of proteins required for helicase activation and replisome assembly. Research proposed herein will lead to a deeper understanding of the mechanism and regulation of DNA replication. We have reconstituted the licensing reaction with purified proteins which will be used to characterise the mechanism of licensing and the mechanism by which licensing is regulated in the cell cycle. We will also use this system to reconstitute events leading to the initiation of DNA replication. We will use genetic and biochemical approaches to characterise the mechanisms by which perturbed licensing causes gross chromosome rearrangements. We will also explore mechanisms involved in regulating the temporal programme of origin firing and how origin firing is regulated in response to DNA damage. Work in budding yeast and mammalian cells will be pursued in parallel to exploit the specific advantages of each system.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon 2020 proposal on 'Restoration of visual perception by artificial stimulation performed by 3D EAO microscopy'.

assistant: **Innovation**

The long-term aim of the investigation is to assess the feasibility of creating an “artificial sense” and, thereby, a possible sensory (visual) prosthetic. While working towards this goal, we will have to address the question of how neural assembly activity relates to subjective perceptions. Finding and understanding these functional assemblies will make it possible to reactivate them in a precise, biologically relevant manner to elicit similar cortical activation as visual stimulation. Recent publications suggest that cortical connectivity can be mapped by two-photon microscopy. Here we want, therefore, to develop a novel 3D Electro-Acousto-Optical microscope for high-throughput assembly mapping. The microscope will be capable of scanning neuronal activity with one order of magnitude higher speed (300-500 kHz/ROI) and simultaneously photoactivate neurons with three order of magnitude higher efficiency (2,500 – 25,000 neurons/ms) than existing 3D microscopes while preserving the subcellular resolution required to simultaneously measure the somatic, the dendritic and axonal computation units in the entire V1 region of the cortex. The microscope will be based on our current 3D AO technology; on novel ultra-fast scanning technologies; new, 10-fold faster AO deflectors; and novel (multi-ROI) scanning strategies. Using our microscope in combination with novel caged neurotransmitters and optogenetic tools, we want to map cell assemblies and to understand how they form larger clusters and how they are associated with visual features. Furthermore, as a proof-of-concept of this grant, we want to restore visual perception by recreating previously mapped assembly patterns with 3D artificial photositmulation in behaving mice and see if the animal responds to the artificial stimulus
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'A dynamic, ultra-stable, random-access RNA retrieval database' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

In order for DNA to become the information storage medium that serves as an alternative to existing digital storage solution technologies information must be stored stably in it with the means to repeatedly access and manipulate parts of the stored data. Only few in vitro approaches are capable to address some of these requirements, while in vivo approaches still produce largely static data storage libraries, which limits the real-life applicability of these technologies. In this proposal we present a new, regeneratable solid state storage system consisting of beads, where information, encoded in single stranded DNA strands, can be added, selectively accessed and removed using enzymes and nucleic acid strands as inputs for the different data operations in isothermal reactions with no loss of material. To achieve this a strand architecture is proposed, where unique Data ID sequences are used on the targeted data strands for performing the intended data operations via enzymatic reactions: addition, deletion of data and transcription-based data access. Furthermore, a variant of this system is proposed that uses RNA molecules for the selective access of the data strands, which we aim to implement in a bacterial data storage system as well where RNA encoding bacteriophages will be used as a non-invasive way to introduce inputs for the data operations, such as random data access and removal.   Finally, we present the use of damage suppressor proteins from extremotolerant organisms complementing the in vitro and in vivo system to provide the long-term stability of DNA observed in resilient biological systems.


**Keywords:** DNA storage, Enzymatic reaction engineering, Bacteriophage engineering