RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Unravelling protein phosphorylation mechanisms and phosphoproteome changes under nitrosative stress conditions in E.coli', explaining its relevance to EU policy priorities under Horizon 2020.

assistant: **Impact**

Currently, we face a global antibiotic resistance crisis aggravated by the slow development of more effective and anti-resistance promoting therapeutical solutions. Protein phosphorylation (PP) has recently emerged as one of the major post-translational modification in bacteria, involved in the regulation of multiple physiological processes. In this MSCA individual fellowship application we aim to bridge the current gap in the field for prokaryotes by unravelling the unknown regulatory role of PP on proteins involved in nitrosative stress (NS) detoxification in the model bacterium E.coli. We propose to examine for the first time both global protein modifications (e.g. phosphoproteomics) under nitrogen species stress, as well as characterize PP in individual proteins involved in NS response. We will construct a network model that reflect the phosphoproteomic changes upon NS in E.coli, that may pave the way for the design of new bacterial targets. Understanding how bacteria respond to the chemical weapons of the human innate system is fundamental to develop efficient therapies. We will pioneer research on the mechanism and the regulation of nitric oxide detoxification proteins already identified as phosphorylated, by analyzing how this modification influences their stability and activity in vitro and in vivo. This project opens up new research paths on bacterial detoxification systems and signalling in general, addressing for the first time the role of PP in these processes. The proposal brings together transversal and scientific skills that will enable the researcher to lead the development of this emerging field and position herself as an expert in the area, and aims at establishing the importance of PP in NO microbial response, a novelty in this field.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon 2020 proposal on 'Illuminating black boxes in the nitrogen cycle'.

assistant: **Concept**

With an increasing world population growth, prediction of climate change on the sustainability and resilience of farming ecosystems is key. Since the nitrogen cycle's role within the farming ecosystem is of utmost importance, and an environmental disturbance leads to farming production loss, understanding the cycle's resilience is crucial. The main actors within this cycle are microorganisms, yet these are ignored when predicting ecological effects to climate change (black box models). Microorganisms do not exist as isolated entities, but are mixed in high numbers, maintaining a diverse number of social interactions created through adaptation and evolution. Microbial-Light will illuminate black box models via the acquisition of a multi-parametric database. Physicochemical disturbances will be applied to a large combinatorial number of mixed microbial populations of well studied nitrifiers. Microbial growth monitoring at microtiter scale will include strain tracking (FISH-flow cytometry), while function (i.e. nitrification) will be determined at a larger scale. The ample data collected will enable elucidation of functional landscapes for each synthetic community under combining environmental stresses. Additionally, the role of microbial interactions (BSocial tool) across disturbance gradients will be sought. Microbial-Light, will engage in modelling individual growth from synthetic mixed population growth, thereby validating the models with previous experimental evidence. Moreover, the optimal social nitrifying community will be coated on tomato seeds, and plant growth efficiency will be compared with uncoated seeds. Parallel to the acquisition multi-parametric database, evaluation of the nitrification potential of poor and rich soils will be tested coupled with microbia
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'ERA-NET for establishing synergies between the Joint Programming Initiative on Antimicrobial Resistance Research and Horizon 2020' under Horizon 2020.

assistant: **Concept**

Antibiotic resistance is a global problem. It is considered by the World Health Organization as one of the three greatest threats to human health for the next decades. ’Enhanced coordination across national boundaries is needed to accelerate the efforts to tackle this grand challenge. Such synergies have been created among 19 countries in Europe and beyond through the EU JPI on Antimicrobial Resistance (JPIAMR). JPIAMR has developed a Strategic Research Agenda (SRA) which defines the strategic and scientific priorities benefiting from co-ordination and co-operation at the European level and mobilised national funds for two joint transnational calls (JTC) 2014 and 2015 based on this.In the ERA-NET Cofund JPI-EC-AMR, an additional € 23 million of national funds will be combined with a highly incentivising EC top-up fund to support a new transnational research programme. The aim of this programme is to unravel the complex dynamics of selection and transmission of antimicrobial resistance (AMR) according to the One Health concept. A multidisciplinary approach is necessary to identify and characterise the determinants that contribute to the spread of resistance at genetic, bacterial, animal, human, societal, and environmental levels, in order to provide scientific knowledge to design and evaluate cost-effective strategies that can decrease transmission of AMR in society.JPI-EC-AMR will launch additional JTCs without EC co-funding under the framework of the SRA. and perform additional joint actions in close collaboration with JPIAMR actions.JPI-EC-AMR will provide a better, quantitative understanding of drug-resistant bacteria in animals, foods, and the environment and to what extent they contribute to the burden of antibiotic-resistant infections in humans. Research in this
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'General principles of representing space and time during automatic formation of visual and auditory chunks and objects' (GEPREST) under Horizon Europe.

assistant: **Objectives**

The process by which humans formulate increasingly complex internal representations from sensory stimuli based on their available knowledge has been investigated traditionally by a somewhat disconnected set of approaches under various labels such as “feature detection”, “grouping”, “figure-ground segregation”, “chunking”, “categorization” and “learning”. We explore the idea that there might exist some general unconscious mechanisms of this pattern formation process that work similarly and repeatedly across different levels of complexity from basic segmentation to complex chunking-based learning and across different modalities. such as vision (typically viewed with spatial processing) and audition (associated with temporal processing). We will follow 4 principles in our work to test this. First, we will focus a) on the auditory and visual modalities, b) with space and time as the two dependent dimensions of comparison, and c) we will test the two endpoints of the complexity scale: at one end, pure tones in audition and single squares in vision easily treated by instantaneous perceptual biases and at the other end, complex time- and space-varying patterns requiring learning. Second, we will perform the equivalent version of each experiment in both modalities to check the generality of the mechanisms that establish the representations of the input. Third, we will use the same testing method across experiments, measuring behavioral sensitivity induced by the resulting representation to quantify the representation’s structure and its EEG correlates. Fourth, we will interpret our results by building a new hypothesis about humans’ internal representation into a Hierarchical Bayesian Model so that we can derive further individual- and population-specific predictions to inspire
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Biochemically modified messenger RNA encoding nucleases for in vivo gene correction of severe inherited lung diseases' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

Surfactant Protein B (SP-B) deficiency and Cystic Fibrosis (CF) are severe, fatal inherited diseases affecting the lungs of ten thousands of people, for which there is currently no available cure. Although gene therapy is a promising therapeutic approach, various technical problems, including numerous physical and immune-mediated barriers, have prevented successful application to date. My recent studies were the first to demonstrate the life-saving efficacy of repeated pulmonary delivery of chemically modified messenger RNA (mRNA) in a mouse model of congenital SP-B deficiency. By incorporating balanced amounts of modified nucleotides to mimic endogenous transcripts, I developed a safe and therapeutically efficient vehicle for lung transfection that eliminates the risk of genomic integration commonly associated with DNA-based vectors. I also assessed the delivery of mRNA-encoded site-specific nucleases to the lung to facilitate targeted gene correction of the underlying disease-causing mutations. In comprehensive studies, we show that a single application of nucleases encoded by nucleotide-modified RNA (nec-mRNA) can generate in vivo correction of terminally differentiated alveolar type II cells, which more than quadrupled the life span of SP-B deficient mice. Together with my working group, I aim to further develop this technology to enhance the efficiency and safety of nec-mRNA-mediated in vivo lung stem cell targeting, providing an ultimate cure by permanent correction. Specifically, we will test this approach in humanized mouse models of SP-B deficiency and CF. Developing and genetically engineering humanized models in vivo will be a critical step towards the safe translation of mRNA based nuclease technology to the clinic. With my competitive edge in lung-transfect
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Mucosal IgM and IgD responses in fish: kinetics, interactions, and secondary/memory responses' will create measurable scientific, societal and economic impact aligned with Horizon Europe.

assistant: **Impact**

The fastest-growing food production sector, aquaculture, faces important challenges because of infectious agents. Vaccination is the most effective way to prevent and potentially eradicate such diseases, but to date, most commercial vaccines are administered by injection methods, which for fish, imply many disadvantages. On the other hand, mucosal vaccines (directly administered to mucosal tissues) offer a promising and cost-effective alternative. In my MSCA project, I aspire to bring the field of mucosal immunology to the next level by applying my expertise in immune anatomy and working in the renowned laboratory of Prof. Carolina Tafalla who specializes in characterizing teleost B cells. Through this collaboration, I will uncover the cellular mechanisms and interactions of IgM and IgD B cells at fish mucosal sites, such as gills, skin, and intestines. In these tissues, plasma-like B cells secreting IgM coexist with IgD+IgM- B cells, cells that have lost surface IgM through an unclear recombination event. These cells secrete IgD, suggesting an important but undefined role for IgD at specific mucosal surfaces. At INIA, I will be trained in flow cytometry and single-cell transcriptomics. I will complement these techniques with my knowledge of immune anatomy to comprehensively unveil the role of these mucosal plasma-like cells upon immunization in rainbow trout. This project will innovatively expedite mucosal immunology research and underline my ambitions to become an independent scientist. I will further sharpen my scientist profile through CSIC’s mentoring programs for young researchers, which will enhance my skills in formulating and managing large-scale projects. I will also supervise students, keeping me engaged in the academic sector. The proposal addresses pressing
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Modelling the Earliest Human Settlement in the Kattegat-Skagerrak Circumference' will create measurable scientific, societal and economic impact aligned with Horizon Europe.

assistant: **Impact**

Modelling the Earliest Human Settlement in the Kattegat-Skagerrak Circumference (MESK) is an interdisciplinary project that will investigate the earliest human settlement along the coastline of Kattegat and Skagerrak in present-day Denmark, Sweden and Norway. This will be done by addressing three fundamental challenges that currently hamper our understanding of the earliest peopling of Scandinavia: (1) Chronological control pertaining to the deposition of the archaeological material, (2) variability in coastal engagement and settlement patterns in regions of varying topography, characterised by dramatic changes due to deglaciation and sea-level change, and (3) the degree to which lithic artefacts associated with the settlements are indicative of cultural differences within the region. Due both to research-historical circumstance and modern organisation of the research sectors, our understanding of the Late Glacial and Early Holocene human dispersal and settlement of Scandinavia continue to be influenced by present-day national and administrative borders. To overcome this issue, MESK will involve the application of reproducible computational methods to pan-regional datasets with the research objectives of (1) analysing coastal settlement patterns relative to the contemporaneous shoreline to assess the coverage and reliability of the archaeological dating method shoreline dating, and (2) employ quantitative methods to taxonomically classify lithic armatures that have traditionally been described qualitatively to define archaeological cultures that are discretely bound in time and space. The project is uniquely positioned to provide a nuanced understanding of how people of the deep past settled in previously uninhabited regions, how they adapted to an at times dramatic sea
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Inhibiting resistance to immunotherapy in Oncology by targeting Netrin-1' (ImmunoNet).

assistant: **Concept**

Immune Checkpoint Inhibitors (ICPIs) have recently revolutionized the treatment and management of cancer. Yet, progress recorded and impact on long-term survival affect only a limited fraction of patients, as most of the cancer patients do not or incompletely respond to ICPIs due to primary or secondary resistance to these treatments.NETRIS Pharma is an innovative biotech company based in Lyon. The company has developed NP137, a humanized monoclonal antibody targeting NETRIN-1, able to drive tumor growth inhibition and alleviate resistance to ICPIs, both impacts already confirmed in preclinical models. The objective of the IMMUNONET project is to conduct a proof-of-efficacy clinical trial assessing the efficacy of NP137 to alleviate suspected resistance to ICPIs. The increased response rate to ICPIs with NP137 would have a major impact on patients having limited treatment options beyond immunotherapies and create significant growth opportunities for NETRIS.


**Keywords:** immune resistance ,immuno therapy,immune check point inhibitors,solid tumors,dependence receptors,humanized monoclonal antibody,clinical trial
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Modelling of low emissions combustors using large eddy simulation (MOLECULES)' (MOLECULES).

assistant: **Concept**

Increasing air transport has increased pressure to reduce aero engine pollutant emissions. More efficient aero engines with lower CO2 emissions show higher combustor pressures and temperatures leading to increased NOx emissions, so new low NOx combustor technology is required. A detailed knowledge of the physical phenomena in the combustor is needed to improve todays low NOx combustors. This knowledge can be provided by validated combustion CFD tools, lowering development cost and time by replacing expensive rig tests. MOLECULES will provide these validated CFD tools to the combustor designer. The method of Large-Eddy- Simulation is used, which is know to be capable of a better representation of highly turbulent flows. The validation tests will focus on typical features like strong swirl and mixing jets. A generic sector combustor will combine these features and provide benchmark CFD validation data.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Mars Analogues for Space Exploration' (MASE).

assistant: **Innovation**

Assessing the habitability of Mars and detecting life, if it was ever there, depends on knowledge of whether the combined environmental stresses experienced on Mars are compatible with life and whether a record of that life could ever be detected. However, our current ability to make these assessments is hampered by a lack of knowledge of how the combined effect of different environmental stresses influence the survival and growth of organisms. In particular, many combinations of stress, such as high radiation conditions combined with high salt and low temperature, relevant for early Mars, have not been investigated. Furthermore, a lack of experimental studies on how anaerobic microorganisms respond to such stresses undermine our knowledge of Mars as a location for life since the planet is essentially anoxic. Even if life can be shown to be potentially supported on Mars, there exist no systematic studies of how organisms would be preserved. MASE proposes to address these limitations in our knowledge and advance our ability to assess the habitability of Mars and detect life. In particular, MASE intends to: - Isolate and characterise anaerobic microorganisms from selected sites that closely match environmental conditions that might have been habitable on early Mars.- Study their responses to realistic combined environmental stresses that might have been experienced in habitable environments on Mars.- Investigate their potential for fossilisation on Mars and their detectability by carrying out a systematic study of the detectability of artificially fossilised organisms exposed to known stresses.Cross cutting aspects of i) optimised methodologies for sample management and experimental process and ii) optimised methodologies for life detection will also be thoroughly conside
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Ferroic Materials for Dynamic Heat Flow Control' (DYNAMHEAT).

assistant: **Innovation**

Tackling climate change is one of the most pressing challenges of our modern society and requires researching new refrigeration and renewable energy systems. Performances of all these systems could be significantly improved if they were combined with solid-state thermal switches and diodes. Current strategies that require to nanostructure materials or to operate in the vicinity of a phase transition, lead to thermal switches or thermal diodes with low efficiencies or not suitable for applications where space is limited. Furthermore, once designed, thermal properties of these elements are set and cannot be modified.My objective is to investigate a fundamentally new mechanism to design compact and efficient thermal switches and diodes. My strategy exploits, in ferroelectric and ferroelastic oxides, the interactions between phonons and spontaneously occurring planar defects known as domain walls. Domain walls can be easily generated, moved, and oriented by application of a small voltage or a small uniaxial pressure, and interact with phonons as defects do. They are thus perfect interfaces to achieve large and reconfigurable anisotropies in thermal conductivities in controlled directions in a fast and reversible way.In this ambitious project, I develop a novel approach to demonstrate a dynamic heat flow control through (i) the reversible engineering of the density of domain walls in desired directions, and (ii) the development of advanced experimental techniques for in-operando thermal characterizations. My multidisciplinary strategy will unravel the interactions between phonons and domain walls to reach higher thermal conductivity variations, and lead to ground-breaking thermal switches and diodes. These thermal switches and diodes will be compatible with a large range of