RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Transgenerational epigenetic regulation of heat stress response' (TRANS-EPIGEN), addressing dissemination and exploitation.

assistant: **Impact**

Abiotic stresses, mainly high temperature, is among the most limiting factor of crops performance and their geographical distribution in European agriculture and worldwide. Although work on temperature tolerance dates many years back, mush less is known about the underlying molecular mechanisms. New evidences point out to long-lasting effect that could be transmitted across developmental stages and even among generations. The TRANS_EPIGEN project will address this issue, focussing on the plants’ reproductive structures that are primary determinants of crop yield and vigour that is of human interest. Adverse conditions during germ cell formation, pollination, seed formation and grain filling account for the bulk of yield losses due to abiotic stresses. Important regulatory steps in these developmental stages are under epigenetic control mechanisms causing transmittable changes in gene expression by modification of DNA and associated proteins. We hypothesize that stress conditions change the interaction between epigenetic regulators and their targets. TRANS_EPIGEN will focus on investigating the influence of heat stress on sexual plant reproduction, aiming at identifying long-lasting epigenetic effects, epigenetic key regulators, their targets, and their interactions in the model plant Arabidopsis thaliana. Thus, the TRANS_EPIGEN proposal will generate novel information relevant to stress physiology and adaptation, epigenetic inheritance, and crop performance under increasingly changing environmental conditions. Identifying genes potentially suitable to improve plant performance under heat stress conditions will pave the way for further research on their orthologues in crop plant aimed at engineering crop plants with better performance after stress exposure, generating un
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Better Contrails Mitigation' under Horizon Europe.

assistant: **Concept**

Aviation contributes to about 5% of the total anthropogenic climate change when including non-CO2 effects, e.g., contrail formation and the impact of NOx emissions on ozone and methane.  Among various non-CO2 effects, the contrail-cirrus radiative forcing is the largest (~2/3) with large uncertainties. The most critical affecting factor is the huge weather-induced variability of the radiative impact of individual contrails. This is the quantity, BeCoM will predict better since the knowledge of the individual radiative forcing is the basis for avoidance of just those contrails that contribute most to the overall climate impact. Once this is standard, it will be possible to formulate adequate mitigation measures and develop policy-driven implementation schemes. BeCoM will address the uncertainties related to the forecasting of persistent contrails and their weather-dependent individual radiative effects. BeCoM focuses on: 1) obtaining a larger and higher resolution database of relative humidity and ice supersaturation at cruise levels for assimilation into numerical weather prediction (NWP) models; 2) providing more adequate representation of ice clouds in their supersaturated environment in the NWP models; and 3) validation of the predictions to determine and reduce the remaining uncertainties of contrail forecasts. To facilitate the assimilation and validation process, BeCoM will develop a novel hybrid artificial intelligence algorithm. Based on the contrail prediction, BeCoM will develop a policy framework for effective contrail avoidance through a trajectory optimization approach. BeCoM will enable a better understanding of contrail’s climate impact and formulate recommendations on how to implement strategies to enable air traffic management to reduce aviation's clima
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Interaction of toxic organotin compounds towards human metallothionein-3' under Sixth Framework.

assistant: **Concept**

Metallothioneins (MTs) are low-molecular-weight metalloproteins (MPs) with high cysteine (Cys) content. They are involved in a wide range of biochemical processes. In human MT-1 and MT-2 isoforms are found in all organs, whereas MT-3 is expressed mainly in the brain. Human exposure to organotin compounds (OTs) stems from their use as polyvinyl chloride heat stabilizers and as marine biocides. Certain OTs have already been detected in blood, liver or urine samples from individuals. Acute exposures can result in either neurotoxicity or immunotoxicity depending on the specific compound involved. Recently, attention has been focused on the biological chemistry of OTs. It has been shown, that Cys is one of the primary biological ligands for OTs. Regarding to its high Cys content, it is likely that main target of OTs in brain cells is MT-3.The objective of this project is to study the interaction of human MT-3 towards four neuro- and immunotoxic OTs detected in drinking water and in biological samples from human b ody. The conditions of the over-expression, purification and the conversion to an air-stable derivative of recombinant MT-3 will be optimised first. Incorporation of OTs into MTs, composition of the resulting new MP(s) will be studied by electrospray ionisation mass spectrometry (ESI MS), inductively coupled plasma atom emission spectrometry (ICP AES), circular dicroism (CD) and ultraviolet-visible (UV-Vis) spectrophotometry. The incorporation of OTs into Zn-MT-3 under physiological conditions will also be studied. Finally, the inductive effect of OTs on the over-expression of MT-3 will be monitored as a control.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Untangling population representations of objects. A closed loop approach to link neural activity to mouse behavior.' (NEURACT) under Horizon Europe.

assistant: **Objectives**

A paramount component of intelligence is our ability to extract useful information in the world through our sensory observations. Object recognition is a fundamental problem in visual perception: every day we depend on our ability to identify objects in our visual environment, and our brain is capable of accomplishing it effortlessly and in a fraction of a second, in spite of immense variation in the sensory information that arrives in our retinas. Understanding the algorithm that the brain uses to do this complex task is a decisive conquest in neuroscience but in order to understand ethologically relevant visual processing, we need to understand how it drives behavior. Despite significant progress characterizing visual processing, we do not understand how the visual system solves visual inference problems in natural environments and we are still far from having a complete understanding of how the brain creates untangled transformation-invariant object representations in the perceptual/visual domain, that can subsequently be used to guide behavior. The proposed research effort aims to (i) create a state-of-the-art behavioral virtual navigation system for mice, (ii) combine it with recent advanced functional brain recording techniques and sophisticated neural data analysis to study how objects are represented in the activity of large populations of neurons across the visual hierarchy and beyond and (iii) causally relate these representations to the behavior of the animal. The outcomes of this project will provide significant insights into the computations used by the mouse visual cortex to extract relevant features from the environment, identify how distinct features are represented across the mouse visual areas and how in turn these representations guide the behavior of
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Combinatorial neuromodulation of internal states' (StateNeuromod).

assistant: **Innovation**

Being sleepy, hungry, afraid, or happy impacts our daily experience and defines our behavior and actions. Such internal states are characterized by unique signatures of activity across neural circuits and are actively shaped by neuromodulators, such as dopamine, serotonin, acetylcholine, and norepinephrine, that are released widely throughout the brain to alter the function of target neural circuits. At any moment, distinct combinations of neuromodulators are being released in each brain region. Changes in the neuromodulator balance have a powerful impact on the brain-wide configurations that underlie internal and behavioral states, while most mood disorders are associated with neuromodulator imbalance, and most therapeutic strategies attempt to restore this balance. However, how combinations of neuromodulators define distinct brain configurations and shape internal states remains unknown. The prefrontal cortex and the hippocampus are two interconnected regions, which are essential for cognitive processing and memory. They receive converging neuromodulatory inputs, and synaptic alterations in these regions are critical for learning, while dysfunction of this pathway is involved in most mood disorders. Despite the vital role of these circuits for brain function, little is known about how combinatorial neuromodulation regulates circuit configuration and coordinates information flow between these regions. In this project, we will combine neuromodulator monitoring with large-scale in vivo electrophysiology, optogenetics, and in vivo genome editing to answer the following questions and resolve the contribution of neuromodulators to the organization of neuronal ensembles and information gating in the hippocampus-prefrontal circuit in behaving mice.1. What is the neuromodulato
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Exploring the bacterial cell cycle to re-sensitize antibiotic-resistant bacteria' (ChronosAntibiotics), addressing dissemination and exploitation.

assistant: **Impact**

Over the next 35 years, antibiotic resistant bacteria are expected to kill more than 300 million people. The need to find alternative strategies for antimicrobial therapies remains a global challenge with several bottlenecks in the antibiotic discovery process. Using Staphylococcus aureus, the most common multidrug-resistant bacterium in the European Union and an excellent model organism for cell division in cocci, we propose: (i) to find new pathways to re-sensitize resistant bacteria. Bacteria undergo major morphology changes during the cell cycle. We hypothesize that these changes generate windows of opportunity during which bacteria are more susceptible or more tolerant to the action of antibiotics. We will identify key regulators of the cell cycle in order to manipulate the duration of windows of opportunity for the action of existing antibiotics. (ii) to develop new fluorescence-based reporters for whole-cell screenings of antimicrobial compounds with new modes of action, including compounds that arrest or delay the cell cycle; compounds that target non-essential pathways that are required for expression of resistance against existing antibiotics and therefore can be used as synergistic drugs for combination therapies; compounds that inhibit the production of virulence factors and compounds that revert persister states that are phenotypically resistant to antibiotics. (iii) to unravel new modes of action of antibiotics by using the constructed reporter strains as powerful tools to learn how antibiotics act at the single cell level.Over the past years, my group has become expert on the biology of S. aureus, has constructed powerful biological tools to study cell division and synthesis of the cell surface and has studied mechanisms of action of various antimicrobial
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Strengthening research in the Croatian Geological Survey: Geoscience-Twinning to develop state-of-the-art subsurface modelling capability and scientific impact', explaining its relevance to EU policy priorities under Horizon 2020.

assistant: **Impact**

This proposal addresses the need to spread excellence and widen participation across the European Union by the twinning of research institutions. We propose to twin the Croatian Geological Survey (HGI-CGS) with two world-leading geoscience research institutes, the Geological Survey of Denmark and Greenland (GEUS) and the British Geological Survey of the United Kingdom Research and Innovation (BGS-UKRI), to significantly strengthen HGI-CGS’s research, and in a number of areas transform its capability. The overarching objective is: to deliver a coordinated and targeted programme of training, knowledge exchange and collaboration, involving world-leading experts, to embed cutting-edge research techniques, tools and knowledge within HGI-CGS, leading to a significant and measurable improvement in their geoscientific research capability. HGI-CGS will benefit from a range of research tools, technologies, software and methods at the disposal of GEUS and BGS-UKRI. However, central to this project is the development of active collaboration and partnership between people; between talented scientists within HGI-CGS and highly productive scientists within BGS-UKRI and GEUS, who in a number of cases are world-leading experts in their field. Through close collaboration of scientists working on a range of geoscience topics, facilitated through scientific workshops, two-way scientific exchanges and bespoke training programmes, the project will support HGI-CGS to strengthen research in four important geoscience subject areas, which are at the core of most world-leading geological surveys and geological research institutes: (i) 3D geological surveying and modelling; (ii) groundwater flow and contaminant transport modelling; (iii) geological hazards; (iv) geothermal energy. The project will
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Mass spectrometry of structural dynamics in secondary membrane transporters' (2007-2013) under Seventh Framework.

assistant: **Objectives**

Secondary membrane transporters are found across the biological kingdom. The structural dynamics of their actions are elusive, as our understanding relies on few X-ray structures. Comparisons of the available structures show that large re-arrangements take place between two domains during transport, with what is generally described as a “rocking-bundle model”. However, dynamic motion between the transport domains remains largely inaccessible to most experimental analysis methods. Here we propose to use a novel mass spectrometric approach that allows the desolvation of intact membrane proteins directly from detergent or lipid environments in combination with ion mobility mass spectrometry to monitor conformational changes in secondary transporters. This study will initially focus on the glutamate transporter GltPh for which structures, inhibitor, mutagenesis and cysteine cross-linking studies are available. We will then apply the same approach to the less-well understood transporters harboring the NhaA-fold. The goal will be to elucidate the conformational dynamics of NhaA, NapA, and ASBT proteins during transport and monitor the effects of substrates, ions, lipids and oligomerization. In summary, this project aims to establish a novel mass spectrometry-based methodology to bridge the gap between structure and function of secondary active transporters.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon 2020 proposal for the project 'Smart logistics for WASTE and recycling operations in European cities'.

assistant: **Objectives**

The key problem in waste collection today is static routes and schedules: truck drivers are driving “blindly” from bin to bin and collecting containers that are either half empty or over filled. This adds up to a large amount of unnecessary costs, such as time spent, gas consumption and greenhouse gas emissions. Globally, over 400 million waste containers are being served by millions of trucks every day, and 50 % of the value in the market is in the logistics. Enevo is a growing Finnish technology company that aims to capitalise on this 12-billion-euro business opportunity and become the #1 supply chain platform company for waste and recycling operations worldwide.As waste management plays a central role in the circular economy, Enevo is a key player in developing more efficient waste collection and management systems. Enevo helps its customers make their waste and recycling operations more efficient, leading to a more sustainable world. Enevo’s vision is to turn all waste in the world into a valued resource.SmartWASTE project is addressing two significant EU-wide challenges:1) optimising transport operations and tackling the environmental and logistical challenges that the European transport sector is facing2) waste management in the circular economy context. The objective of SmartWASTE proposal is to scale-up and expand the service into new European regions by piloting the solution with potential customers in 10 large scale pilots. Through piloting, Enevo gains important feedback that is provided back to product development to improve Enevo's offering and operations to be better suited for large scale regional expansion.The proposal’s activities aim at creating a solid foundation for Enevo’s successful business in European market and accelerate its expansion globally.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'The normalisation of natural philosophy: how teaching practices shaped the evolution of early modern science' (NaturalPhilosophy).

assistant: **Innovation**

Early modern natural philosophy underwent dramatic transformations that completely reshaped its conceptual framework and set of practices. The main contention of my ERC project is that teaching practices had a decisive and ‘normalising’ impact on the progressive dissemination, adaptation and selection of rival conceptions of natural philosophy. Normalisation occurs when historical actors collectively present certain tenets as crucial for the study of a discipline, and thus prescribe them as a necessary subject for teaching and learning. The overall aim of this ERC project is to determine and explain how the process of normalisation embedded in teaching practices shaped the evolution of early modern natural philosophy. To study normalisation, it is necessary to operate a systematic comparative investigation of hundreds of works through which natural philosophy was taught, learned and reshaped, both within and outside universities. The size of this corpus defies the traditional method of close reading used by historians of philosophy and science. I will meet this challenge by organically integrating close reading with digital ‘distant reading’. I will digitally transcribe a corpus of approximately 500 early modern works on natural philosophy, published in Britain, France and the Dutch Republic. Using digital tools to investigate how the networks of authors and concepts of natural philosophy co-evolved over time will allow me to identify textual excerpts that are representative of historical trends. By analysing these excerpts with close reading and assessing them against the digital results, I will determine and explain how normalisation shaped the evolution of natural philosophy. This project will boost the integration of digital approaches in the history of philosophy a