RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Artificial evolution  of a novel multifunctional human polymerase' (EVOPRIMPOL) targeting Horizon 2020.

assistant: **Abstract**

The product of the human gene ccdc111, PrimPol, has been recently identified and characterized by the group of Professor Luis Blanco (CBMSO, Madrid, Spain). This protein is capable of performing both priming and polymerase activities in human cells, contributing to DNA integrity and maintenance during its replication. As an enzyme involved in tolerance to DNA damage, its malfunction gives rise to human diseases. Thus, it might become a target for cancer therapy. In order to exploit the benefits derived from knowledge of PrimPol, a deeper structure-function study must be done. These studies can not be accomplished using standard site-directed mutagenesis protocols and further characterization due to variety activities that this protein displays, the novelty of its discovery and the absence of a solved crystal structure. Thus, EVOPRIMPOL will be a novel, fast and reliable approach to study the role of critical amino acids involved in the different activities shown by the enzyme. This approach is based on the in vitro directed evolution of human gene ccdc111, but unlike other directed studies performed in polymerases, where genes are mutated randomly, in EVOPRIMPOL we will use a new rational design to select those residues that will be randomised; selecting them after the phylogenetic study of all members of the protein superfamily containing PrimPols as well as by homology sequence studies using different DNA polymerases and primases from other superfamilies. Chosen residues will be randomised following an iterative mutagenesis scheme, where evolved variants are subjected to new rounds of mutagenesis mimicking the natural evolution. Thus, improved PrimPols will be obtained with better catalytic properties. Biochemical study of these variants will provide us information ab
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon 2020 proposal for the project 'Quantum Nanomaterials by Twistronics'.

assistant: **Objectives**

Van der Waals heterostructures, assembled from 2D materials, offer unprecedented opportunities for the material design by layer-by-atomic-layer construction of artificial solids. The potential of this approach is enormous, as demonstrated by the hundreds of patents and thousands of research publications appearing annually in the field.This proposal will explore a new avenue in design of quantum solid state systems by employing a mutual rotation between adjacent crystalline planes, “twist”, to pattern their properties on the nanometre length scale. This opportunity has emerged due to my recent pioneering work with twisted layers of transition metal chalcogenides and ground-breaking technological achievements in ultra-high vacuum fabrication instrumentation. I will transform the emerging field of “twistronics” by the in-situ fine-tuning of moiré superstructure and making pioneering studies of novel quantum and strong correlation phenomena in 2D materials accessible for the first time. In particular, I will explore different regimes of lattice reconstruction in 2D semiconductors to engineer and study (1) quantum many-body states of electrons and (2) design confined quantum states defined by piezoelectric and pseudomagnetic nanotextures in semiconducting and charge density wave 2D materials. Moreover, we will create and study world-first twisted heterostructures with (3) superconducting and (4) superconducting and magnetic 2D materials, aiming to create strong periodic nanotextures of their electronic states.  The realisation of my ambitious research programme is only possible due to our unique world-first UHV-technology which will allow assembly of high-quality twisted van der Waals heterostructures, and which we will develop to enable new dynamic twist-angle studies and l
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon Europe proposal on 'Handling and Sorting delicate objects using Acoustophoresis'.

assistant: **Concept**

Sorting on conveyor belts is a fundamental process across industries, ensuring efficiency and quality in pharmaceutical labs, recycling, and agriculture. In agriculture, precision seed sorting is critical, driving a €2.9B global market growing at 4.5% CAGR through 2030. However, current pneumatic sorters are high-maintenance and costly, gravity sorters lack precision for similar-density seeds, and robotic pick-and-place systems, though precise, are too slow and expensive for industrial-scale operations.AcoustoFab is introducing a step-change in sorting technology through its patented ultrasonic phased array transducer system, which uses precisely controlled sound waves to manipulate and eject seeds from a conveyor belt. Unlike traditional methods, this high-speed, contactless approach enables multi-category sorting, eliminates mechanical wear, and reduces operational costs. Originally developed under the ERC Advanced Grant “Interfaces” for interactive media applications, our acoustic manipulation technology has unexpectedly revealed a high-impact opportunity in AgriTech, unlocking new levels of precision and efficiency in seed sorting.Rising global food demand, climate change-driven variability in seed quality, and increasing sustainability regulations make high-precision, low-waste sorting solutions more critical than ever. This proof-of-concept funding will allow us to advance and benchmark our technology against seed industry KPIs, validating its speed, accuracy, and efficiency while developing prototypes that demonstrate industry feasibility and commercial potential.Beyond agriculture, our contactless sorting technology can revolutionize high-purity metal recovery in recycling, such as copper extraction from solar panels, enhancing efficiency and reducing waste. Lev
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Yeast cell factory for mRNA bioproduction' in the context of a Horizon Europe call.

assistant: **Objectives**

Messenger RNA (mRNA) vaccines have become a game-changer in the fight against COVID-19, providing an exit strategy to the global crisis of our age. mRNA is now considered a promising biopharmaceutical for a vast array of medical applications. Currently, in vitro transcription (IVT) is the only available technology for mRNA production; however, it relies on a complex supply chain and costly purification process. Reliable large-scale IVT production is challenging and high production costs are an issue for low-income countries, and this will be even more critical for clinical mRNA applications requiring high doses. Yscript’s groundbreaking nature lies in the generation of a specific mRNA bioproduction process in yeast, integrating innovative extraction and purification processes, representing a complete shift of paradigm compared to IVT production and a revolutionary new use of yeast. Although yeast possesses a similar mature mRNA structure as humans and is recognized as safe for pharmaceutical production, mRNA bioproduction and isolation have never been attempted. Yscript will provide groundbreaking technological improvements by: i) decreasing the mRNA production supply chain; ii) increasing the purity yield of long mRNA by avoiding premature termination occurring during IVT; and iii) guarantying mRNA stability through integrated extraction-purification processes. This will ensure the generation of high-quality mRNA therapeutics, while streamlining production and reducing costs. Yscript brings together world-class expertise in molecular biology, biophysics, biotechnology, chemistry, chemical engineering, mRNA therapeutics, yeast Ty biology, bioproduction and purification processes. The project will significantly advance the current state of the art of mRNA manufacturing.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'ABIOtic Synthesis of RNA: an investigation on how life started before biology existed' (ABIOS), addressing dissemination and exploitation.

assistant: **Impact**

The emergence of life is one of the most fascinating and yet largely unsolved questions in the natural sciences, and thus a significant challenge for scientists from many disciplines. There is growing evidence that ribonucleic acid (RNA) polymers, which are capable of genetic information storage and self-catalysis, were involved in the early forms of life. But despite recent progress, RNA synthesis without biological machineries is very challenging. The current project aims at understanding how to synthesize RNA in abiotic conditions. I will solve problems associated with three critical aspects of RNA formation that I will rationalize at a molecular level: (i) accumulation of precursors, (ii) formation of a chemical bond between RNA monomers, and (iii) tolerance for alternative backbone sugars or linkages. Because I will study problems ranging from the formation of chemical bonds up to the stability of large biopolymers, I propose an original computational multi-scale approach combining techniques that range from quantum calculations to large-scale all-atom simulations, employed together with efficient enhanced-sampling algorithms, forcefield improvement, cutting-edge analysis methods and model development. My objectives are the following:1 • To explain why the poorly-understood thermally-driven process of thermophoresis can contribute to the accumulation of dilute precursors. 2 • To understand why linking RNA monomers with phosphoester bonds is so difficult, to understand the molecular mechanism of possible catalysts and to suggest key improvements. 3 • To rationalize the molecular basis for RNA tolerance for alternative backbone sugars or linkages that have probably been incorporated in abiotic conditions.   This unique in-silico laboratory setup should significantly
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon Europe proposal for the project 'Stochastic quantum gauge theories'.

assistant: **Objectives**

This proposal aims to solve central open problems in the mathematical foundation of quantum gauge theories (QGTs), an important challenge comprising the Yang-Mills (YM) Millennium Prize Problem. A key outcome of the proposal will be the first constructions in finite volume of 2- and 3-dimensional non-exactly solvable QGTs, with a view towards the physical case of 4 dimensions.The principal tools that will be developed and used to address these problems are in the field of rough analysis, in particular singular stochastic partial differential equations (SPDEs). Singular SPDEs appear widely in the study of dynamics with randomness and have seen revolutionary progress in the past decade. By developing new rough analytic methods applicable to QGTs, the proposal will push the frontiers of rough analysis, in particular studying discrete approximations of SPDEs, introducing novel geometric solution theories, and linking SPDEs with random matrix theory.My research has shown that the stochastic quantisation equations of YM (SYM) can be renormalised in a geometrically faithful way,which has already revealed new properties of the exactly solvable 2D YM measure. This is strong evidence that rough analytic techniques can bring new light to the study of QGTs and render their construction in 2D and 3D finally within reach.The proposal is split into the following three long-term projects.1. Two-dimensional theories: solve and identify the invariant measure of SYM for non-trivial principal bundles; prove large N convergence of SYM; construct the non-Abelian YM-Higgs measure in finite volume.2. Three-dimensional theories: give the first construction of the 3-dimensional YM measure in finite volume; prove a discrete version of the BPHZ renormalisation theorem in regularity structures.3. A
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'In Home High Speed Multimedia Network based on IEEE 1394' goes beyond current state-of-the-art under Fifth Framework.

assistant: **Innovation**

Digital in-house technology enables new services, applications and communication structures. Digital audio video and control data require new kinds of networking with high data throughput and reliability. The User and the market demand more user- friendliness. The purpose of the project is to develop and integrate different home devices and functions inside the home into a comprehensive in-home network concept. New interfaces, user software and related middleware will be developed. The research activities ill cover the issues of interoperability, usability, easy access to other networks and reliability. The 1394 networking is the basic technology for the project. It offers high data rates until 400 Mbit/s und in future 3200 Mbit/s. Furthermore the bus performance fits to the needs of convergence between PC and Consumer electronics.OBJECTIVESThe main objective of the project is the development of a next generation in-home network. It will integrate different technologies with regard to new in-house buses and prototypes of end devices including consumer electronics, white goods, and telecommunication devices in order to provide convergence between the different technologies. The main characteristics of this in-home network will be reliability, high interoperability, self-configuration, usability, scalability, performance, plug and play. A furteer objective is to provide a set of new applications by utilizing the developed in-home network and a new generation of user interfaces. In particular the following prototypes will be developed: Multimedia TV Terminal, IEEE 1394 Satellite Front end, Multimeaia-Domotic Controler, Home Navigator including various Home Services. All devices will communicate with each other over an IEEE 1394 data backbone.DESCRIPTION OF WORKThe consorti
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Fourth Framework proposal on 'Life Science Trace Gas Exchange Facility'.

assistant: **Concept**

Principal characteristics of the facility and of the support offered to users: Access, training and support will be granted to researchers from Life Science to carry out trace gas exchange experiments with state-of-the-art laser-based photoacoustic trace gas detectors which, due to their extremely high sensitivity, make measurements possible which cannot be done with standard instrumentation. We offer these facilities to researchers engaged in experiments where a quantitative change of small gas emissions forms an important asset to determine the character and the timing of the observed processes. The facility is suitable for detection of biologically interesting molecular gases like ethylene, ethane, methane, water vapor, acetaldehyde, ethanol, other small hydrocarbons and nitrogen and sulfur containing compounds below the ppb-level (1 ppb = 1 part per billion = 1:104). In the last few years the potential of the photoacoustic trace gas detectors has been demonstrated; they are proven to be indispensable in high quality research performed in agriculture and biology, in the field of plant physiology, microbiology, soil science, entomology and toxicology. The strength of the technique resides in the possibility to perform sensitive, on-line, non-invasive detection of trace gases under rapidly changing environmental conditions (e.g. temperature, light, humidity, O2-, N2-, and CO2-levels, etc.). In Europe such a measuring facility is unique and definitely not commonly used by Life Scientists; because of the required expertise and high operational costs its availability is restricted. Quantity of access being offered and number of users who may benefit: For the next 3 years it is estimated that 14-16 research groups per year will use the facility, i.e. in total 45 visits of
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Contribution of calcium-independent isoforms of phospholipase A2 in the pathogenesis of Duchenne muscular dystrophy' (FRZDMD).

assistant: **Innovation**

The applicant proposes a 2-year project to be performed under the supervision of Prof Bernheim, a neuromuscular disease expert at the University of Geneva. The goal of this project is to determine the role of isoforms of calcium-independent isoform phospholipase A2 (iPLA2) in cytosolic calcium entry and muscle cell death during Duchenne muscular dystrophy (DMD).DMD is the most severe case of muscle-wasting diseases of the muscular dystrophies family. It affects 1 in 3500 children, and is caused by mutations in dystrophin mRNA leading to the absence of the protein in skeletal muscles from affected patients. As dystrophin is an important player in muscle maintenance, its absence leads to skeletal muscle degeneration. To date, this is an incurable disease with 100% of fatality. Affected boys first lose the ability to walk, then breath, and finally die before being 30 years old. Although many mutations have been characterized, more research is needed to understand the molecular and signaling mechanisms underlying DMD, and increase the opportunities to find new therapeutic targets. Recent experiments have highlighted the importance of calcium entry in muscle fibers death. iPLA2 appears to have a role in this elevated cytosolic calcium, and has been shown to be upregulated both in DMD patients and dystrophic mdx mice. The applicant will 1) establish the implication and roles of the different iPLA2 isoforms in DMD by comparing calcium responses and muscle cell-death in wild-type (wt) and mdx muscle fibers, and 2) determine the effects of specific inhibitors on muscle cell survival.This project could have beneficial issues towards developing new strategies to counteract cytosolic calcium imbalance in muscle fibers from DMD patients, and thus potentially alleviate their symptoms
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'hybridFRET - deciphering biomolecular structure and dynamics' (2014-2020).

assistant: **Innovation**

To understand and modulate biological processes, we need their spatiotemporal molecular models. In this project we propose to build these models by a holistic approach. The recent methodological and technical advances in fluorescence spectroscopy and microscopy as well as in multi-scale modelling of complex biochemical systems set the stage to tackle cross-fertilizing challenges in biophysics, biochemistry and cell biology. The applicant proposes to develop a novel integrative platform for a Molecular Fluorescence Microscope (MFM) to achieve ultimate resolution in space (sub-nanometer) and time (picoseconds) for characterizing structure and dynamics of proteins. MFM will combine Multi-parameter Fluorescence Detection with Computational Microscopy (molecular dynamics and coarse grained simulations) in a hybrid approach, first, to derive a complete molecular description of all fluorescence properties of the tailored dyes in proteins (objectives 1 and 2) and, second, to utilize this information in simulations to report on the protein properties (objective 3). In this hybrid approach high precision FRET measurements are the core experimental technique (hybridFRET). The MFM will allow us to tackle the central biophysical question of how intra- and intermolecular domain interactions modulate proteins' overall structure, dynamics, and thus ultimately function (objective 4). In this proposal we will apply MFM to two prototypic proteins of significant medical relevance. The combination with Multi-parameter Fluorescence Image Spectroscopy will exploit the ultimate resolution of the MFM for molecular protein imaging in live cells. To follow and ultimately understand biological processes, we need their spatiotemporal models of the integrative fluorescence spectroscopy platform. Unt