RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Type I interferon as an adjuvant: immune mechanisms and preparation of new vaccines against the Epstein-Barr virus' goes beyond current state-of-the-art under Fourth Framework.

assistant: **Innovation**

The Biotechnology programme includes the important task of developing vaccine research in Europe (area 5). Although effective vaccines for a number of infectious diseases are available, the immune mechanisms involved in the protection are not fully understood and additional efforts are needed for developing second generation vaccines against classical infectious diseases as well as new vaccines against yet unchallenged pathogens. A crucial issue in vaccine research is the identification of new adjuvant factors and mechanisms involved in the generation of a long-lasting protective immunity. Recent studies indicate that type I IFN (especially IFN-alpha) is an important factor for the activation and persistence of a protective CD8-T cell-mediated immune response against both infectious agents and tumours, thus suggesting that this cytokine can be considered a powerful vaccine adjuvant. The general aims of our study are: i) to characterise the adjuvant effects of type I IFN in well defined mouse models; ii) to study the advantages of using IFN as an adjuvant in an animal model closely resembling the EBV infection in man (represented by immunocompetent mice infected with the MHV-68 virus); iii) to develop new vaccines against EBV to be tested in suitable human/mouse models in SCID mice. We will use different types of vaccine strategies, including direct DNA immunisation with the relevant genes as well as the use of recombinant viruses and transduced dendritic cells expressing viral peptides and IFN. These studies should lead to consider IFN-a as an effective adjuvant for some human vaccines. The choice of EBV as the initial human virus for this combined vaccine strategy relies on specific considerations (importance of CD8+ T cells in the control of EBV infection, existence o
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Engineering T cells and B cells for Immunotherapy using V(D)J recombination' (VDJtargeting), addressing dissemination and exploitation.

assistant: **Impact**

T cell engineering has shown clinical success mainly in haematological cancers, but scaling up is challenging due to reliance on ex vivo manipulations. In addition, B cell engineering has not shown therapeutic efficacy to date. Here, we propose a novel immunotherapy approach, allowing safe and efficient engineering of B cells and T cells, both ex vivo and in vivo. We will use adeno associated vectors (AAV) to integrate chimeric antigen receptor (CAR) or T cell receptor (TCR) genes into loci coding TCR chains and to integrate antibody (Ab) genes into loci coding Ab chains. Previously, we used AAV facilitated gene targeting in vivo to ameliorate genetic diseases in mice. For lymphocytes we develop “VDJ targeting”: A promoterless receptor/Ab gene flanked by recognition signal sequences (RSS) will be inserted into the endogenous locus by the recombination activating gene (RAG) complex during V(D)J recombination. Only developing lymphocytes, expressing RAG, will incorporate the receptor/Ab gene, which will thus be expressed in potent naïve cells from the strong endogenous promoter. Targeted developing cells are subjected to negative selection, thus reducing risk of adverse autoimmunity. Lack of promoter reduces spurious expression and oncogenic risk upon rare off-target integration. Targeting endogenous loci may allow allelic exclusion. In B cells it may allow utilizing the endogenous constant region to express a B cell receptor and, upon activation, a secreted Ab. Activation may be accompanied by proliferation and affinity maturation, including somatic hypermutation and class switching, to allow a potent immune response, memory retention and diminished antigenic escape. Where controlled autoimmunity is desired, we will engineer B cells to inducibly secret an auto-Ab. We wil
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Evaluation of methods for defect assessment of power plant components at high temperature' demonstrating excellence, impact and implementation quality for a Fourth Framework call.

assistant: **Abstract**

Objective of this project is to provide a comprehensive assessment procedure for the high temperature response of structures. This procedure is intended to establish a prenorm for assessing the lifetime of components in power plants. In the project the already existing procedures (R5) and standards BSI PD6539, French procedure RCC-MR and ASME-Code Case N-47 will be evaluated and brought together to get a comprehensive procedure. It will be a main task to formalize a consistent procedure based on the different approaches and complexities of the procedures. This involves also the necessity to get the best results possible. After the evaluation the new procedure will be tested by comparing the results with experiments and with case studies taken from power plants. Training content (objective. benefit and expected impact) The objective is a better understanding of the international assessment procedures in this field as well as it will contribute to my PhD-thesis. The expected impact is in the form that the new assessment method will allow higher security standards and high financial benefits to the power plants through a more precise inspection plans. Links with industry / industrial relevance (22) Materials and case studies will be provided by industry mainly AZT Allianz, GKM Mannheim and Siemens AG. The results will be disseminated to industry.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Thermostable transaminases for the synthesis of pharmaceutical building blocks' under Seventh Framework.

assistant: **Concept**

Biocatalysis is considered a key component for the development of a sustainable chemistry in Europe by the European Commission, CEFIC and Biotech Industries Association. Indeed, the use of enzymes as biocatalysts in the chemical and pharmaceutical industries has been constantly growing in the last years.Aim of this project is to screen and obtain novel thermostable omega-Transaminases (TAs) from the metagenomes of (hyper)thermophiles. Furthermore, the novel TAs will be characterized and their biocatalytical application will be investigated.TAs catalyze enantioselective reductive amination reactions of ketones and represent an attractive option for the synthesis of chiral amines, valuable building blocks for the synthesis of several pharmaceutical compounds.In the respect of other enzyme classes, the array of available TAs is still very restricted. The search of novel TAs will provide suitable biocatalysts for the industrial development of a larger set of synthetic processes.The overall concept of this project is to exploit metagenomics for the discovery of a new generation of thermostable TAs from the hot terrestrial environments.The complementarity of the skills provided by the Applicant and the Host will ensure the success of the project. In fact, the Host is an expert in metagenomic studies and has already an in-house collection of environmental samples from various geothermal regions of the world available for library preparation. On the other side, the Applicant has a 7-years proved experience in applied biocatalysis, acquired by joining the Biocatalysis group at ICRM-CNR (Milan, Italy), one of the world leading research groups in the field. This experience will be exploited for the characterization and development of the novel biocatalysts.It must be pointed out t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Metal-Organic Framework Field-Effect Transistor Arrays for Chemical Sensing' under Horizon Europe.

assistant: **Concept**

Detecting volatile organic compounds (VOCs) at low concentrations is an important but challenging task with a range of applications, including checking food freshness, monitoring indoor air quality, and diagnosing lung conditions through breath analysis. In all of these areas, high-performance yet compact VOC sensors are needed. While compact VOC sensors exist, they suffer from limited lifetimes and low selectivities, and often produce questionable data. Metal-organic frameworks (MOFs) with pores of molecular dimensions are excellent materials to adsorb VOC analytes from air, even at very low concentrations. The MOFFET project targets the integration of MOFs with transistor technology to realize a novel type of VOC sensor. In addition, the modular nature of MOFs and microfabrication will be combined to realize an ‘electronic nose’ demonstrator for the selective detection in mixtures of VOCs. If successful, the project will contribute to novel, non-invasive medical diagnostics, checking food freshness, and monitoring air quality.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'New methods for the divergent synthesis of non-natural amino acids under aqueous conditions' (DSNNAA).

assistant: **Innovation**

The ultimate goal of this research is to develop reliable chemical methodology that can allow the modification of specific amino acid side-chains in peptides in a highly divergent manner providing access to a wide variety of mutants" containing non-natural amino acids at a specific site from a single precursor. The approach will involve the preparation of a variety of boron-containing amino acids, either as precursors, or by derivatisation of existing amino acid residues in peptide chains, and then use of the Suzuki reaction to introduce the desired non-natural side-chains."
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Seventh Framework proposal on 'Predictive modelling and simulation in mechano-chemo-biology: a computer multi-approach'.

assistant: **Concept**

Living tissues are regulated by multi-cellular collectives mediated at cellular level through complex interactions between mechanical and biochemical factors. A further understanding of these mechanisms could provide new insights in the development of therapies and diagnosis techniques, reducing animal experiments. I propose a combined and complementary methodology to advance in the knowledge of how cells interact with each other and with the environment to produce the large-scale organization typical of tissues. I will couple in-silico and in-vitro models for investigating the micro-fabrication of tissues in-vitro using a 3D multicellular environment. By computational cell-based modelling of tissue development, I will use a multiscale and multiphysics approach to investigate various key factors: how environmental conditions (mechanical and biochemical) drive cell behaviour, how individual cell behaviour produces multicellular patterns, how cells respond to the multicellular environment, how cells are able to fabricate new tissues and how cell-matrix interactions affect these processes. In-vitro experiments will be developed to validate numerical models, determine their parameters, improve their hypotheses and help designing new experiments. The in-vitro experiments will be performed in a microfluidic platform capable of controlling biochemical and mechanical conditions in a 3D environment. This research will be applied in three applications, where the role of environment conditions is important and the main biological events are cell migration, cell-matrix and cell-cell interactions: bone regeneration, wound healing and angiogenesis.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Palaeoenvironments of Human Behavioural Evolution in Africa' in the context of a Horizon Europe call.

assistant: **Objectives**

The emergence of Homo sapiens' behavioural complexity represents a fundamental milestone in the evolution of humankind. Still, the causes of this critical transformation remain debated. For the African Middle Stone Age (MSA, ~300 000–40 000 years) a key question is whether and how climate variability contributed to the increasing behavioural complexity in modern humans. However, behavioural-environmental hypotheses remain untestable because the existing palaeoclimatic datasets are spatially, stratigraphically, and causally disconnected from the archaeological record.PIONEER will overcome this impediment by developing a new analytical framework that allows testing of previously untestable hypotheses and thereby advances our understanding of human behavioural evolution. To achieve this, cutting-edge analytical (leaf wax isotope analyses) and computational approaches (climate- and agent-based modelling (ABM)) will be combined with African archaeology. Fundamental to my approach are high-resolution climate records from within the archaeology-bearing sediments, thus directly connecting environmental- and archaeological records.  PIONEER will test behavioural-environmental hypotheses by interlinking several activities:1) establish unprecedented high-resolution datasets of past vegetation and palaeohydrology changes from five key cave sites, inhabited by early Homo sapiens, located along the South African coast2) create a high-resolution spatial representation of the environments experienced by our ancestors from novel climate simulations3) explore likelihoods for different behavioural-environmental scenarios via ABMAlthough PIONEER focuses on the South African MSA, my approach is applicable to most archaeological timeframes and locations. Consequently, PIONEER will transform
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'A truly-rapid, one-minute test system for the dairy industry to assess raw milk quality, detect sub-clinical mastitis and monitor udder health, reducing antibiotic usage and environmental impact' (LacDetect) under Horizon 2020.

assistant: **Objectives**

Mastitis is an infection of the udder in dairy cattle. It is a major problem in the dairy industry, costing European farmers tens of millions of Euros annually. Milk somatic cell count (SCC) is used as a main indicator of mastitis, as the number of somatic cells in milk increases in response to infection in the udder. Krysium Technologies has developed and patented LacDetect® – an innovative, portable, economic test system allowing reliable and truly rapid detection of sub-clinical mastitis on-farm based on enumeration of SCCs via ATP-luminescence technology. This allows farm workers and veterinarians to make quick decisions on herd management and avoid significant costs associated with clinical mastitis. Furthermore, as SCC is also used as a measurement of milk quality, dairy producers base their milk pricing policy significantly on SCC values in raw milk. LacDetect® will enable recording of SCCs on-site for payment purposes. LacDetect® is at Technology Readiness Level 6. Initial testing has repeatedly shown results from our prototype test system compare very favourably with those from a major milk laboratory. In this Phase I project we intend to review the specification of LacDetect®, to ensure that production costs are reduced and that the end-product specification meets user requirements. We will re-contact and add commercial partners for field testing and commercializing plus explore other routes to market. On completion of Phase I we intend to progress to a Phase II project during which we will carry out final product development, scale-up manufacture, validation and final customer trials prior to commercial launch of LacDetect® initially in key EU markets. Completion of the overall project will deliver a one-minute, on-farm raw milk test system enabling EU farmer
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'SCIentific gateway Based User Support'.

assistant: **Objectives**

SCI-BUS will create a generic-purpose gateway technology as a toolset to provide seamless access to major computing, data and networking infrastructures and services in Europe including clusters, supercomputers, grids, desktop grids, academic and commercial clouds. SCI-BUS will elaborate an application-specific gateway building technology and customisation methodology based on which user communities can easily develop their own customised gateways. SCI-BUS will use the gateway technology and customization methodology to create 11 application-specific gateways customized for various types of user communities (represented in the project) and to provide gateway services for these communities including astrophysics, seismology, helio-physics, bio-science, computational chemistry, biomedical communities, PireGrid SMEs. community, Blender community, citizens. web-2 community, DCI application developer community, business process modeling community. SCI-BUS will establish production gateway services for the represented user communities and will attract further user communities by training and by other forms of disseminations activities. SCI-BUS will use the established production customized gateways as best-practice case studies based on which other communities can build their own customized gateway. SCI-BUS will provide gateway development, operation and maintenance support for the new communities to build and operate their own customized gateways. SCI-BUS will provide user support for application developers and end-users to develop and run new DCI applications based on the developed gateways. SCI-BUS will develop business models to enable the commercial exploitation of the developed technologies and to guarantee the sustainability of the gateway services developed in the pro
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Shaping Hellenic National Identity between 18th and 19th centuries: how European intellectuals steered Greek and European History' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

What role did textual exchanges (epistles, journal essays and newspaper articles) between Italian philhellenes and (Italian)-Greek intellectuals refugeed in Italy (due to long-term cultural/political bonds) and Switzerland (as host country for many Greek exiles) play in setting ground for the 1821 Greek Revolution? How did the reflections of these intellectuals on the idea of Greekness, on contemporary historical events and on the illuministic ideas contributed to the process of national identity negotiation underlying the Revolution? Answering these questions is crucial to fully understand the role of the Greek Revolution in shaping modern Europe. HeNaId will offer the first in-depth study of these textual exchanges, focusing on texts by major (Italian-)Greek intellectuals with a focus on the poet Ugo Foscolo as a leading figure among those. While the ideological roots of Greek Revolution are generally studied from historical-philosophical or iconographic perspectives, HeNaId will fill a gap in scholarship by adopting an interdisciplinary approach combining archival and bibliographical research with a philological-literary investigation to 1) map out the political and cultural networks of (Italian)-Greek intellectuals between 18C and 19C across Greece, Italy and Switzerland; 2) study the texts exchanged within these networks  to understand their degree of influence; 3) highlight the so-far neglected role played by Foscolo in promoting and orientating these cultural networks. Employed methodologies will be greatly improved  through an outgoing phase at the leading Foscolo study group of the University of Fribourg, a secondment at the Laboratory in Italian History and Historiography at the Kapodistrian University of Athens and thanks to the main host University of Bergam