RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Microfluidic EV Quantification for Point-of-Care CRC diagnostics' demonstrating excellence, impact and implementation quality for a Horizon Europe call.

assistant: **Abstract**

The μQuantEV project aims to revolutionize the landscape of colorectal cancer (CRC) diagnostics through the development of a groundbreaking Point-of-Care (PoC) platform. Leveraging the emerging potential of extracellular vesicles (EVs) as robust biomarkers for CRC, this interdisciplinary endeavor combines cutting-edge microfluidic technology with advanced analytical methods to enable the sensitive, rapid, and cost-effective detection of CRC-specific EVs.By designing, fabricating, and characterizing a microfluidic EV enrichment module, the project pioneers an innovative approach for the selective capture of CRC-associated EVs from patient-derived samples. Simultaneously, a state-of-the-art fluorescence-based EV detection and quantification module is developed to provide accurate and real-time analysis of the captured EVs. These two modules are seamlessly integrated to create a Lab-on-a-Chip diagnostic platform capable of detecting CRC at early stages with exceptional sensitivity and specificity.Furthermore, μQuantEV places a strong emphasis on translational research, collaborating closely with clinical partners to validate the platform's performance using patient samples. The project also incorporates extensive open science practices to foster collaboration, transparency, dissemination of research outcomes.Upon successful completion, the μQuantEV platform holds the potential to revolutionize CRC diagnostics, offering a cost-effective, non-invasive, and efficient alternative to current screening methods. Beyond CRC, the technology developed in this project can pave the way for the application of EV-based diagnostics in various other diseases, driving advancements in personalized medicine and improving healthcare outcomes.


**Keywords:** Extracellular vesicles, microfluid
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Dissection of the mammalian transcription termination mechanism by CRISPRi technology.' (2014-2020), addressing dissemination and exploitation.

assistant: **Impact**

RNA polymerase II (RNAPII) transcribes protein-coding genes in eukaryotes. The proper RNAPII transcription termination is crucial for generation of functional mRNAs, and then proteins. Termination, or stop of RNA synthesis, may occur before or after mRNA 3’-end cleavage and polyadenylation. In the last decade many details have been acquired about mRNA maturation, but still little is known about the termination per se. In this proposal I will redress this problem.One of the proposed transcription termination triggers is RNAPII elongation complex pausing downstream of mRNA maturation signal. I will test this hypothesis, using sequence-specific protein binding in the living cells.In the proposed project I will combine cutting-edge CRISPRi/dCas9 technology with classical molecular biology, high-throughput sequencing and computational biology. This approach will allow me to address my research objectives: (1) to elucidate the role of elongation complex pausing in transcription termination and (2) to characterise the induced pausing in terms of stress-resistance. Also by implementation of this project I will develop a bioengineering tool to induce elongation complex pausing.This timely approach, taking advantage of innovative technologies, will allow me to elucidate previously unknown details of RNAPII transcription termination in mammals and expand our knowledge about this process. Importantly, my approach will allow studies on the chromosomal genes in the living cells, making the results unique and valuable. Implementation of the proposed project will enhance my proficiency by improving and diversifying my skills in experimental and computational science as well as boosting my transferable skills, such as scientific communication and writing, project management and networki
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Exploiting Glycosylation of Colorectal Cancer for the development of improved diagnostics and therapeutics' to be submitted under Horizon 2020.

assistant: **Abstract**

Colorectal cancer (CRC) is a major worldwide cancer burden with about 1.4 million cases in 2012 and an annual mortality of approximately 700,000. Early detection is crucial as treatment is most efficient in early stages where population based screenings could substantially reduce incidence and mortality.Current screening techniques are invasive or lack sensitivity and specificity. Moreover, the molecular mechanisms leading to the formation of different antigens suggested as CRC biomarkers and potential therapeutic targets are poorly understood, especially with regard to carbohydrate-based molecules, such as glycans. Enhancing our understanding of the structure-function relationship of glycosylation in CRC could lead to the discovery of improved diagnostic and prognostic biomarkers and pave the way for nov-el therapeutic targets.Building on an established network of analysts with many years of experience in (glyco)proteomics and biomarker research, in collaboration with colleagues in the field of glycobiology and glyco-immunology, GlyCoCan will develop new methods, and use current state of the art methods, to investigate the role of glycosylation in many different aspects of CRC.The GlyCoCan multi-disciplinary network will principally be a training programme with a substantial industrial focus on technology transfer and teaching of internationally adopted biopharma regulations (GMP, ISO9001, ICH guidelines). The underlying specific research objectives will be addressed within individual ESR projects, giving rise to a generation of ESRs whose main focus is investigating and tackling the challenges of the role of glycosylation within CRC and other diseases. The network will address the currently unmet need for glycosylation researchers with an inter-disciplinary perspectiv
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'ELECTROCHEMICAL DNA-BASED SENSORS FOR IMMUNOTHERAPY MONITORING' (IMMUNO-MONITORING), addressing dissemination and exploitation.

assistant: **Impact**

During the initial part of the ERC-Consolidator Grant PRO-TOOLKITS we have successfully demonstrated a novel approach for antibodies detection. The platform uses antigen-conjugated synthetic DNA strand to achieve the rapid and inexpensive detection of target antibodies.  Our platform offers several advantages (low-cost, single-step, rapid) in comparison of other methods for antibody detection and is so versatile that can be used also for the detection of Bispecific Antibodies (BsAb). On the basis of interactions with pharmaceutical companies and following a market analysis we have found that there is a growing need for similar approaches that can allow to monitor the efficacy of immunotherapies. This is especially true for immunotherapies using BsAb as there are no standard analytical methods for these targets. Given these considerations, the goal of this project is to evaluate the commercial potential of our innovative platform for immunotherapy efficacy monitoring with particular focus on the detection and characterization of BsAb.  During the project we will 1) Fully characterize the DNA-based antibody sensor for the detection of a model BsAb (Anti EGFR/Anti HER2 BsAb) in terms of analytical performances (i.e. sensitivity, specificity, stability etc.) with direct comparison with other used techniques; 2) Prepare a Manufacturing Plan for producing/testing the Ab-switch; 3) Establish an IP strategy for patent filing and maintenance; 4) Determine a business and commercialization planning.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Welfare for Migrant Factory Workers: Moral Struggles and Politics of Care under Market Socialism' to be submitted under Horizon 2020.

assistant: **Abstract**

The transformation of China and Vietnam from centrally planned economies into today’s market economies has been fuelled by the labour of millions of migrant factory workers from rural areas. These countries started reforming at the turn of the 1980s, embracing marketisation while remaining under the Communist party’s political monopoly, a system now commonly termed market socialism. In what seems to be a reversal of the wider trends of austerity, there has been rapid expansion of social protection, much like what has been happening in other Global South contexts. While the shifts indicate state and societal responses to the social conflict and tension induced by marketization, they in turn have been foregrounded by the politics around how different groups of people should be cared for, politics that are part of wider moral struggles between actors in the new economy. Given their underclass status and their salience as a social force, the question of the migrant factory workers’ welfare is critical for the understanding of the on-going shifts in these countries’ welfare systems. WelfareStruggles is aimed at uncovering the politics of care underlying the provision of welfare for migrant factory workers in China and Vietnam. It does so through a comparative investigation that has two ground-breaking features: 1) the combination of ethnography with comparative social policy analysis, and 2) a translocal approach that takes into account the workers and their families’ negotiation for welfare across the city and the countryside. The comparison is expected to generate path-breaking knowledge about the variable moral dynamics of market socialist welfare. The knowledge will be essential for understanding the momentous yet little-known transformations of Global South welfare and
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'The role of A20 in dendritic cells and mast cells in the allergic immune response' (2007-2013).

assistant: **Concept**

Asthma is a chronic inflammatory airway disease in which the transcription factor NF-kB plays a crucial role. NF-kB controls the activation of mast cells and dendritic cells (DC) that contribute to disease pathogenesis. Activation of NF-kB is tightly regulated by ubiquitination and deubiquitination of signaling intermediates. Studies in the host laboratory identified several deubiquitinating enzymes like A20 (also known as TNFaip3) and its binding partners to set the tone for NF-kB activation. A20 deficient mice succumb from an autoimmune inflammatory disease, through exaggerated responses induced by Toll like receptor (TLR) triggering, precluding a detailed analysis of the function of A20 in vivo. In the current proposal we will study the effect of cell-specific deletion of A20 on allergic airway inflammation, employing recently generated conditional A20 deficient animals, and crossing them with DC-specific and mast cell-specific deletor mice. In mast cells and DCs, we will study various ubiquitination and deubiquitination signaling events in the NF-kB pathway following activation with TLR agonists, cytokines and house dust mite allergen (HDM). Subsequently, the effects of cell-specific deletion of A20 on cellular function will be ascertained in vitro, using the most recent techniques in cell biology and cellular immunology. Next, we will study the effects of cell-specific deletion of A20 on the function of mast cells and DCs in vivo, focusing specifically on mast cell degranulation, passive cutaneous anaphylaxis, DC activation and parameters of T cell activation and differentiation. Finally, mast-cell or DC-specific A20 deficient mice will be subjected to asthma protocols driven by ovalbumin and HDM and salient features of asthma like eosinophilia, Th2 cytokine produc
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'CleanMOULD: Advanced low friction and fretting-resistant diamond-like coating solution' (2014-2020) under Horizon 2020.

assistant: **Objectives**

Fretting wear is the limiting factor in the life of many plastic injection moulds. Each time that the mould is closed during itsmoulding cycle, the contacting surfaces “adjust” to each other and undergo microscopic vibrational movements that slowlydamage these surfaces, leading to severe tear and particle release. Ultimately, the mould needs to be scrapped afterrepeated regrinding, thus significantly affecting overall mould service life. Since longer productive lifecycles further dilute theheavy upfront investments in casting moulds in injection moulding production pipelines, reducing the impact of frettingcorrosion is a fundamental need transversal to all manufacturing industries relying in this type of productive process.Building on CemeCon Scandinavia extensive experience in solving tribological problems in injection mould’s operationalenvironments, cleanMOULD is a novel coating solution, based on a multi-layer DLC formulation that jointly optimizes boththe surface hardness and lubricity to minimize the impact of fretting wear. The current project is positioned to untap a verysignificant business opportunity by preparing our next-generation DLC coating for full market roll-out, while also thoroughlydocumenting its performance through large demonstration, in fully operational manufacturing pipelines within the premises ofkey industry players in the injection moulding industry.cleanMOULD has the potential to become a key enabling technology transversal to entire plastic industry. Overall, return-of- investment for cleanMOULD users is expected to be over 20x the expected coating costs, thus providing a very compellingvalue proposition. In addition, cleanMOULD represents a huge business opportunity for CSA, opening the perspective ofadding approx. €14 million over the 5-
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Sixth Framework proposal for the project 'Advanced training in hybrid technologies for nano-structured composites'.

assistant: **Objectives**

Considerable research efforts still need to be devoted to the integrated processing of nanomaterials and composite nanostructures. Cutting-edge results are nowadays awaited from special field- and pressure-assisted synthesis and processing technologies dev eloped during the past few years. Processing under extreme pressure and temperature conditions leads to important changes of the density, electron configuration and free energy of the constituent phases.The resulting chemical equilibrium changes allow the access to a wide range of new compounds. External pressure can further induce reactions between chemical components at much lower temperatures, thus facilitating the synthesis of metastable phases with valuable modified properties. Special techniques such as field-assisted or microwave sintering are examples of highly promising approaches towards the synthesis of bulk materials with ultra-fine microstructures.A special and promising feature of these new technologies is the ability to consolidate dissimilar materials (metallic particles, polymers, ceramics) into complex nanostructured systems, such as composite bulk nanomaterials, thin films, coatings and nanosize clusters deposited on substrates. Hybrid technologies that integrate soft-chemical synthesis with mechanical activation, pulsed current, ultrasonic, laser or microwave radiation processing are at the forefront of technological development.The versatility and innovative character of these new hybrid technologies result from the unique design of the processing sequence that alternates physical and chemical synthesis steps, accessing a variety of ways to influence the end-product material properties. The proposed training and research programme further addresses the essential question of ultra-fast energy trans
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Urban Gut Microbiome: Unravelling the microbial contribution to wildlife adaptation to urban environments (UGMi)' (UGMi) under Horizon Europe.

assistant: **Objectives**

Urbanization imposes novel environmental stressors that can drive rapid evolutionary responses in wildlife. While vertebrate studies suggest that host-associated microbiomes play a critical role in adaptation to urban environments, their functional contributions in invertebrate hosts remain poorly understood. This project, The Urban Gut Microbiome (TUGM), investigates how gut microbiomes contribute to adaptation in urban settings using the moth Xestia xanthographa as a model system. By integrating microbial ecology, evolutionary biology, genomics, and microbiology, TUGM will (i) assess how urbanization shapes gut microbiome diversity and function, (ii) evaluate whether gut microbiome drive phenotypic plasticity and gene expression via reciprocal microbiome transplants in germ-free larvae, and (iii) assess microbial contribution to host phenotypes through isolate reinoculation assays under simulated urban scenarios. Through a combination of field sampling, metagenomics, transcriptomics, and manipulative experiments, this project aims to provide mechanistic insights into the role of gut microbes in mediating host adaptation to urban environments. TUGM thus advances beyond descriptive studies, offering a novel, interdisciplinary framework to explore the ecological and evolutionary dynamics of host–microbiome interactions in a rapidly urbanizing world.


**Keywords:** Microbial ecology,  Urbanization, Adapatation, Entomology
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Web Accessibility Directive Decision Support Environment' (WADcher).

assistant: **Concept**

One of the many challenges people with various degrees of disabilities face is their difficulties in accessing mainstream products and services thus being excluded from benefiting from Web content and services on an equal basis as people without disabilities. Marginalising people with disabilities on this level is a critical and problematic issue, especially in today’s “Information Society”, where access to information should be equally available to all. While these challenges have been identified (also by EC with the recently Web Accessibility Directive which was entered into force on 22/12/2016), they have not been addressed yet efficiently.In this context and building on ongoing tools and work, WADcher will research, develop and demonstrate an innovative Decision Support Environment for large scale hybrid web accessibility assessment services. WADcher aims to build a large scale infrastructure by integrating extended and enhanced existing web accessibility solutions, and by also making them customizable to the needs of different stakeholders in EU member states. The minimisation of costs and development time along with increased scalability, accessibility and usability for all types of end-users will be also analysed. Specifically, the WADcher Innovation Action will be based on the following pillars: 1) build a large scale accessibility assessment infrastructure; 2) develop and demonstrate advanced decision support tools for assisting developers and designers to create accessible assets; 3) implement a web accessibility observatory platform for hybrid combination of automatic/expert reviews; 4) validate WADcher results under a significant number of pilots and users and a coherent Web Accessibility Conformance methodology; 5) develop methods and tools with which organ