RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Identifying the neuronal basis of self-concept in the human medial prefrontal and medial temporal brain regions' demonstrating excellence, impact and implementation quality for a Horizon 2020 call.

assistant: **Abstract**

Humans have a remarkable ability to construct a complex conceptual representation of their own identity and personality. This self-concept plays a crucial role in everyday life; however, its neurobiological basis remains unknown. Here, we will use the state-of-the-art method of intracranial electroencephalography (iEEG) to characterize the neuronal representation of self-concept at the level of single-neurons in the medial prefrontal and medial temporal brain regions. IEEG will be recorded from awake human subjects, which is possible because a subset of neurosurgical patients requires placement of intracranial electrodes to identify the focus of epileptic seizures. We will conduct our studies during this monitoring time, with no added risk to the patients. Our data will provide unprecedented insights into the neuronal basis of the human self. Our findings will be of great interest to scientists in a broad range of disciplines, including neuroscience, psychology, artificial intelligence, neurology, psychiatry, as well as to the general public. The supervisors are world-leading experts in the neural self-representation (Prof. Ehrsson) and iEEG (Prof. Fried). Their laboratories conduct excellent research, as documented by multiple high-impact publications. The host institution (Karolinska Institute) and the partner institution (University of California, Los Angeles) are among the best universities in the world. Together, the fellowship provides an exceptional opportunity for the experienced researcher to become a leader in his field; he will learn the unique method of iEEG and he will collect the very first data on the neuronal basis of the conceptual self.


**Keywords:** self-concept,  declarative memory,  intracranial electroencephalography,  humans,  single-neuron, res
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon Europe proposal on 'Next Generation Vaccine Platform Leveraging Skin Immunity to Provide Disease-Modifying Treatment of Parkinson’s Disease'.

assistant: **Innovation**

Parkinson’s Disease (PD) is a major neurodegenerative disorder with no established treatment modalities capable of modifying disease pathology, and no means of early diagnosis. Vaccines targeting aSyn aggregates are a promising route to disease-modifying therapy for PD, but the current generation of PD vaccines utilise conventional formulations, which are limited in their immunogenicity and require substantial quantities of adjuvant to achieve efficacy. NEXGEN’s proprietary WISIT vaccine platform is the first of the novel class of gluconeoconjugate vaccines (GNCVs), which are administered intradermally and specifically formulated to leverage skin dendritic cells (DCs) to generate substantially stronger and more specific immune responses than conventional vaccines. These stronger immune responses allow substantial reduction in adjuvants, while simultaneously increasing therapy efficacy. NEXGEN will identify and characterise candidate WISIT constructs targeting aSyn (PD-WISITs) and develop a novel extracellular vesicle (EV)-based biomarker assay that enables early diagnosis of PD using liquid biopsies, suitable for point of care use. Safety and efficacy of PD-WISITs will be demonstrated preclinically, before being translated to first-in-human Phase I/Ib clinical trials, along with the novel EV-based biomarker assay. The results of NEXGEN will be the extraordinary accomplishments of cheap and effective disease-modifying treatment of early PD and a novel biomarker assay to diagnose and guide prodromal/early PD treatment. Further still, GNCV technology will be clinically demonstrated, which has the potential to be transformative to the treatment of a wide range of additional diseases, resulting in far-reaching impacts to the health of millions.


**Keywords:** Therapeutic Va
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Optimised integration of Combined Computational Imaging and image processing for transparent Micro-DEVices' (OCCIM-DEV) targeting Horizon 2020.

assistant: **Abstract**

A PhD level candidate with expertise in computational imaging and image processing is sought by TSD to integrate and optimise the performance of a metrology instrument being developed for the inspection of transparent and turbid micro-devices. The skills of the candidate will be critical in reaching the goals of inspection rate and of adaptability of the instrument to tailored specifications required for a successful introduction to the market. The  kills of the candidate will complement the optical and mechanical engineering skills set existing within TSD, giving the candidate great opportunities of personal development into high end metrology instrument development.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Mapping the retrotransposon-mediated layer of neuronal gene regulation in the human genome' (PRIMATE-TE-IMPACT).

assistant: **Innovation**

Throughout evolution, the human genome has been attacked by retrotransposons, parasitic DNA elements that spread through our genome by a copy-paste activity. I previously showed that SVA elements, the youngest class of retrotransposons in our genome, harbour a strong gene-regulatory potential which is normally repressed by KRAB zinc finger protein ZNF91 (Jacobs et al., 2014, Nature).  However, for reasons unknown, repression of retrotransposons is much less efficient in the human brain, resulting in activation of the enhancer potential of SVA elements spread throughout the human genome. The importance of these SVA insertions for the evolution of human neuronal gene-regulatory networks, and how many genes have come to depend on SVA's regulatory influence, remains elusive. In this research program, I will use ‘cortical organoids’; three-dimensional brain tissues derived from human and primate stem cells, to investigate how recent SVA insertions have impacted human neuronal gene expression. Furthermore, I will investigate how changes of the epigenetic landscape in neurons affect the activity of retrotransposons in our genome and the influence they have on nearby neuronal genes. Finally, I will explore the possibility that loss of epigenetic silencing of retrotransposons is responsible for dysregulation of genes associated with neurological diseases. Preliminary findings suggest a potential role for retrotransposons in susceptibility loci for Alzheimer's and Parkinson's disease. Finding further support for this in the current research program, will form the basis of a novel concept which explains how changes in the epigenetic landscape can uncover a dormant genetic predisposition to disease.


**Keywords:** retrotransposons
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Development of researchers mobility policy guidelines for the region of Western Balkans' goes beyond current state-of-the-art under Sixth Framework.

assistant: **Innovation**

The main objective of the project is the development of guidelines related to researchers mobility policy in the region of Western Balkans. More specifically these guidelines will aim at the enhancement of the policy of the countries of the region to preve nt the brain-drain of researchers and generally to support the research policy of the countries in the area, in terms of strategic planning for the exploitation of human resources, becoming an area of free mobility of researchers. Other objective is to cre ate synergies acting in close cooperation and complementary to the ERAMORE Network, in terms to keep researchers in their region or to direct them within Europe, fostering the realization of the European Research Area in the region. A consortium composed b y CERTH as the coordinator, academic participants representing all countries from Western Balkan, will run the project. CERTH belonging to a neighboring Member State, (Greece) and participating in the ERAMORE Network as Bridgehead Organizations of the Nati onal Mobility Centres Networks will assure synergies with the ERAMORE Network and the European Mobility Portal as well. To achieve this general scopes, the project will focus on the following activities: 1.To carry out an extended mapping exercise to ident ify the national web links from the Western Balkan countries which could be useful for the European Mobility Portal, and all the main stakeholders (Research organizations, Industry, and Ministries or Administrative Services) involved with researchers mobil ity. 2.To review the existing mobility policy in EC and Western Balkan region 3.To prepare informative material and mobility guides for all countries 4.To organize an awareness campaign for project's results implementation
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Preclinical studies in mouse hematopoietic stem cells for gene therapy of Wiskott-Aldrich Syndrome' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

Wiskott-Aldrich Syndrome (WAS) is a primary X-linked rare disease (1:250.000 mainly children) caused by mutations in the was gene. Gene Therapy (GT) results in Primary Immunodeficiencies (PID) have been particularly successful due to advances in gene modification of hematopoietic stem cells (HSCs). Currently there is a clinical trial which has developed an ex vivo approach that uses a HIV-derived lentiviral vector to transfer genes into autologous CD34+ HSCs from WAS patients. This clinical study, initiated in 2010 in London by the team of Professor Thrasher, is also conducted in Paris and in Boston. Our laboratory has developed therapeutic lentiviral vectors (LVs) for GT of WAS (WW y AWW) (Martin et al. 2005; Frecha et al. 2008) that express WASP phisiologically in T cells of WAS patients and restore normal functions of T cells, dendritic cells and macrophages WASP-deficients. Also, the transduction of WASP deficient cells with WW confers a growth selective advantage.Given that the HSCs are the therapeutic target for WAS GT, the first objective of this project is to check the capacity of AWW, WW and the therapeutic vectors currently in use in clinical trial (NCT01347242) in the phenotipic restauration  of WAS knockout mice (WASKO). For that purpose, we will transduce mHSCs of WASKO mice with these LVs and inject them in WASKO irradiated mice to the analysis expression, in terms of the WAS restore capacity of different hematopoietic populations. The second objective will be to improve safety and efficiency of gene transfer vectors for WAS gene therapy. We will include insulator sequences (INS) in our therapeutic SIN-LVs expressing was cDNA through its own promoter. New AWWINS y WWINS vectors will be compared in term of efficiency and safety using WAS-deficient T cells a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'De- and reconstructing virulence strategies of fungal plant pathogens' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

Fungal pathogens are enormous threats to plants, causing tremendous losses in worldwide crop production. Mechanistic understanding of fungal virulence is crucial to developing novel plant protection strategies in sustainable agriculture.  Biotrophic pathogens colonize living plant tissue and reprogram their hosts to stimulate proliferation and development of infection structures. To promote infection, fungal pathogens secrete sets of virulence proteins termed “effectors” in a spatiotemporal program. Many economically relevant biotrophs like rusts and powdery mildew fungi are obligate pathogens. These organisms cannot be grown in culture and are not amenable to reverse genetics, which is a severe constraint for current research. In contrast, the biotrophic smut fungi have a haploid yeast stage, which allows simple cultivation and genetic modification. The causal agent of corn smut disease, Ustilago maydis, is one of the best-established model organisms for fungal genetics. This project aims to utilize the excellent genetic accessibility of U. maydis to approach a previously impossible, pioneering enterprise: the synthetic reconstruction of eukaryotic plant pathogens. In a first step, fungal virulence will be deconstructed by consecutive deletion of the U. maydis effector repertoire to generate disarmed mutants. These strains will serve as chassis for subsequent reconstruction of fungal pathogenicity from different sources. A combination of transcriptomics and comparative genomics will help to define synthetic effector modules to reconstruct virulence in the chassis strains. Deconstruction of U. maydis virulence will identify a complete arsenal of fungal virulence factors. Reconstruction of virulence will show how effector modules determine fungal virulence, including tho
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon Europe proposal for the project 'Genomic characterization of co-evolutionary dynamics along elevation gradients'.

assistant: **Objectives**

Species partnerships that have shaped life on Earth for millions of years are now under unprecedented threat from climate change, yet we lack fundamental knowledge about how these relationships evolve and adapt. This project addresses a critical gap in evolutionary biology by investigating the genomic basis of coevolution—the reciprocal evolutionary changes between interacting species—and how environmental variation affects these dynamics across landscapes. I will uncover how coevolutionary partnerships respond to environmental gradients and climate change by combining cutting-edge genomics with field ecology, using the moth Hadena compta and its host plant Dianthus sylvestris as a model system in the Swiss Alps. While ecological theory predicts that coevolution varies spatially, creating ""hotspots"" of intense interaction and ""coldspots"" of weak interaction, the genomic mechanisms underlying this variation remain unknown. This knowledge gap severely limits our ability to predict how species partnerships will respond to rapid environmental change. I will (1) map coevolutionary hotspots and coldspots along Alpine elevation gradients, (2) quantify the relative contributions of environmental adaptation versus species interactions using population genomics, and (3) predict future coevolutionary dynamics under climate change scenarios. The study integrates field samples, environmental and genomics data with population and ecological genomics across multiple elevational sites, plus ecological  experiments with the secondment lab. This fellowship will establish me as a leader in coevolutionary genomics while providing critical insights for predicting ecosystem responses to climate change. The project will advance fundamental evolutionary theory and inform conservation strat
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for '2D Materials Assisted Nanoscopic Mapping of Proton fluxes in living cells' in the context of a Horizon Europe call.

assistant: **Objectives**

Proton channels in cells and the corresponding transmembrane proton fluxes are critical for many cell activities. Recent studies reveal that expression levels of proton channel Hv1 and its activity are highly associated with the development of many diseases, including cancer spreading, disrupted bone metabolism, and stroke, which makes proton channel an interesting therapeutic target. Yet, the full understanding of the mechanism of proton flux-related disease development is still under development partially due to a lack of direct observation method of the proton fluxes in living cells with high spatiotemporal resolution. To tackle this issue, we propose to develop a high throughput label-free super-resolution imaging method to map proton fluxes in cells by using two-dimensional hexagonal Boron Nitride (hBN) substrate with proton-activated emitters. Very recently, my colleague and I found that surface defects in hBN can be optically activated by protonation in aqueous solutions resulting in intermittent emission that allows single-molecule localization analysis. The high photocounts from the emitters lead to localization with precision as high as 7 nm. Using such surface defects as an array of proton sensors, proton fluxes from proton channels in cell membranes in close proximity can be detected label-free. In this project, we will (1) artificially introduce and characterize defects in hBN that are optimized for proton sensing purposes; (2) test the photophysics of defect emitter in physiological conditions; (3) demonstrate proof-of-the-concept super-resolved mapping of Hv1 proton channel activities in vivo and in vitro.Via this project, I will further develop my core competence in 2D materials, single-molecule sensing, microscopy and more importantly learn to design an
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'rapid screening and identification of illegal Drugs by IR Absorption spectroscopy and gas Chromatography' (DIRAC), addressing dissemination and exploitation.

assistant: **Impact**

The goal of this project is to develop an advanced sensor system, that combines miniaturized Gas Chromatography (GC) as its key chemical separation tool, and Hollow-Fiber-based Infra Red Absorption Spectroscopy (HF-IRAS) as its key analytical tool to recognize and detect illicit drugs, key precursors and potential derivatives. The DIRAC sensor will be developed to: 1) be used on the field primarily by customs officers for controls at the EU external frontiers and by law enforcement personnel for intra-Community checks as a rugged and hand-portable unit; 2) perform rapid detection of key chemicals; 3) reject interferents with minimal false positive alarm rate; 4) perform advanced data analyses such as similarity evaluation between the chemical structure of the unknown sample with that of controlled/illicit substances. Currently, GC-IRAS (through FTIR implementation) is, together with GC-MS (Mass Spectrometry), the most powerful technique for the identification and quantification of amphetamines. However, so far GC-IRAS has been implemented only as bench-top instrumentation for forensic applications and bulk analysis down to milli- and micro-gram quantities. In DIRAC, the use of silicon-micromachined GC columns, solid state lasers, and hollow fibres IR, will allow to develop a GC-IRAS sensor that features hand-portability and prompt response –for field operation– and is capable to perform both bulk analysis and trace analysis with nano-gram sensitivity. The DIRAC sensor will further feature a) an advanced sampling device, that separates the analyte from larger amounts of interfering materials (dust, skin particles) by electrostatic charging; and, b), an advanced silicon micro-machined pre-concentration device, capable to treat sequentially both volatile ATS substances and
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Tuning graphene-based moiré materials with strain' (StrainMoire), addressing dissemination and exploitation.

assistant: **Impact**

The StrainMoiré project aims to advance moiré materials by experimentally studying magic-angle twisted bilayer graphene (MATBG) under controllable uniaxial strain. Despite its ubiquity, the effects of strain in moiré heterostructures are largely unexplored. Leveraging my expertise in the fabrication and the physics of moiré materials and the host group’s expertise on strain engineering, we aim to control strain and study its impact on the structural and electronic properties of MATBG. In particular, we will focus on the breaking of the symmetries of MATBG by the formation of the moiré superlattice and the applied uniaxial strain. We will fabricate MATBG on flexible substrates to study its strain-dependent properties, combining two complementary approaches. First, I will use advanced force microscopy to image strain-induced symmetry breaking in the moiré superlattice under applied uniaxial strain. Then, I will study the emergence of the nonlinear Hall effect in MATBG, enabled by strain-induced symmetry breaking, via low temperature electrical transport measurements.


**Keywords:** Twisted bilayer graphene, strain engineering, topology, flexible electronics, force microscopy