RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Reconstruction of specialized metabolite evolution through molecular switches' (ROSE).

assistant: **Concept**

Novel traits can provide fitness advantages and drive evolution. New specialised metabolites are traits that arose in different plant lineages, and even replaced existing metabolites. Why, and how almost universal metabolic pathways can be replaced in entire groups of species by biochemically distinct pathways without persistence of any species producing both metabolites remains unknown. Components of gene regulatory networks that control such pathways might act as regulatory switches that flip between pathways.The replacement of the widely conserved red pigment anthocyanin by the biochemically distinct betalains offers a unique opportunity to understand the integration of novel traits into existing regulatory systems. Notably, no plant species producing both anthocyanins and betalains has yet been identified, but both show similar environmental responses. The unification of systems biology with population and molecular genomics allows to elucidate the role of gene regulatory networks and regulatory switches in metabolite evolution.Given the great importance of understanding evolutionary innovation and the potential use for metabolic engineering, our work promises to be groundbreaking and have profound impact on many different fields of evolutionary and genetic research.Specifically, our work plan includes the following aims:I. Identify regulatory networks and switches that enabled the exchange of metabolic pathways II. Analyze the fitness consequences of reciprocal pathway exchange and regulatory switchingIII. Reveal the short-term selection consequences of metabolites during the domestication of food cropsUnderstanding how metabolic pathways can be exchanged in plants will provide insights into the important embedding of evolutionary innovation into existing systems w
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'A Bayesian Framework for Cellular Structural Biology'.

assistant: **Objectives**

The functioning of a single cell or organism is governed by the laws of chemistry and physics. The bridge from biology to chemistry and physics is provided by structural biology: to understand the functioning of a cell, it is necessary to know the atomic structure of macromolecular assemblies, which may contain hundreds of components. To characterise the structures of the increasingly large and often flexible complexes, high resolution structure determination (as was possible for example for the ribosome) will likely stay the exception, and multiple sources of structural data at multiple resolutions are employed. Integrating these data into one consistent picture poses particular difficulties, since data are much more sparse than in high resolution methods, and the data sets from heterogeneous sources are of highly different and unknown quality and may be mutually inconsistent, and that data are in general averaged over large ensembles and long times. Molecular modelling, a crucial element of any structure determination, plays an even more important role in these multi-scale and multi-technique approaches, not only to obtain structures from the data, but also to evaluate their reliability. This proposal is to develop a consistent framework for this highly complex data integration problem, principally based on Bayesian probability theory. Appropriate models for the major types data types used in hybrid approaches will be developed, as well as representations to include structural knowledge for the components of the complexes, at multiple scales. The new methods will be applied to a series of problems with increasing complexity, going from the determination of protein complexes with high resolution information, over low resolution structures based on protein-protein inter
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Stress Resilience and Network-Feedback Training' goes beyond current state-of-the-art under Horizon 2020.

assistant: **Innovation**

Acute stress has a profound impact on cognitive functioning: it raises alertness for threat, yet it impairs our ability to think clearly. Repeated exposure to stressors is furthermore a critical transdiagnostic factor in etiology, relapse, and chronification in almost all psychiatric disorders. We know from animal work at the cellular level how stressors trigger a neurochemical cascade that alters properties of widespread neuronal populations. A critical gap in our knowledge, however, is how such cellular effects translate to the level of large-scale neural systems which implement higher-order cognition. Here, I propose a novel framework for understanding such alterations as shifts in network balance: I hypothesize that acute stress causes dynamic shifts in resource allocation at the level of large-scale networks. First, I will leverage recent advances in network connectivity modeling to characterize the spatiotemporal dynamics of such shifts during acute stress and recovery. Using wearable biosensors and mobile applications, I aim to identify which neural markers predict resilience to stress in real life. Second, I will cross-validate these markers in a patient group characterized by high stress sensitivity. Third, to investigate how rapid network shifts are generated, I will examine the distinct roles of noradrenergic and dopaminergic neuromodulatory systems. Fourth, I will test the hypothesis that cognitive functions supported by one network can be disrupted by shifting balance towards another. Finally, I will develop a network-based implementation of functional MRI neurofeedback to train stress-sensitive participants to adaptively reallocate neural resources during acute stress. When successful, this project will yield 1) unprecedented insight into how our brain ada
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon Europe proposal for the project 'Supramolecular Self-Replicating Antimicrobials'.

assistant: **Objectives**

This project aims to address the urgent need for new antimicrobial agents by developing a conceptually new strategy to damage bacterial membranes with supramolecular fibres.Dormant (non-assembling) precursors will accumulate in the membrane of bacteria for in situ switch-on of self-assembly and membrane lysis. Specific metabolic routes of bacteria, absent in mammalian cells, will be used to transport supramolecular precursors into the membrane of bacteria with full cell specificity. Once in the membrane, precursors will combine to generate self-assembling peptide amphiphiles (PAs). Importantly, once formed, the supramolecular assemblies can catalyse their own synthesis in bacterial membranes, causing a dramatic acceleration in PA production and antimicrobial fibrillation. This unprecedented antimicrobial design borrows concepts from cooperative self-assembly, supramolecular autocatalysis and rational peptide design, leading to a self-amplified therapeutic action.This will be the first self-replicating antimicrobial that can make copies of itself to boost membrane damage once activated in target pathogens. Co-assembly of PAs and their precursors will create catalytic microenvironments by templation of reactive building blocks.Overall, this project will lay down structural and mechanistic determinants for biological membrane interfacing with targeted and self-replicating supramolecular nanomaterials.In a broader picture, the REPLICATE technology aims to spark a re-conceptualisation of drug design by drawing the attention to supramolecular therapeutics, which may open new exciting opportunities in biomedicine not accessible from traditional single-molecule drugs.


**Keywords:** Self-assembly, peptide amphiphiles, supramolecular autocatalysis, self-replication, nanofibres,
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Implementing Fuel Cells and Hydrogen Technologies in Ports' (H2Ports) under Horizon 2020.

assistant: **Objectives**

Hydrogen is an energy carrier with great potential for clean, efficient power in transport applications. Hydrogen can be obtained from different sources, which in combination with fuel cells it can improve energy efficiency especially when hydrogen is produced by renewable energy sources. The action proposed tries to introduce hydrogen as an alternative fuel in the port industry.The H2Ports project is an Action aligned with the needs and objectives of the European Commission and the port industry. The aim is to provide efficient solutions to facilitate a fast evolution from a fossil fuel based industry towards a low carbon and zero-emission sector. Hydrogen has been proved in other logistics and transportation sectors as a solution to power machinery and vehicles, therefore the action proposes different pilots to bridge the gap between prototypes and pre-commercial products:• The first prototype will comprise a reach stacker powered with hydrogen and tested under a real life trial, in a Port Container Terminal.• The second prototype will comprise a yard tractor equipped with a set of fuel cells. The design will enable the tractor to perform different operations like container horizontal transport or ro-ro loading/unloading operations.• The third prototype will comprise a mobile Hydrogen supply station, which will provide the needed fuel under the appropriate thermodynamic conditions for guaranteeing the continuous working cycles of the abovementioned equipment.The H2Ports project would also have a transversal objective that consists on developing a sustainable hydrogen supply chain at the port, coordinating all actors involved: customers, hydrogen producers, suppliers, etc. The expected results of the project are to test and validate hydrogen-powered solutions in the po
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'FLEXIBLE RISERS FOR DEEP WATERS' in the context of a Fourth Framework call.

assistant: **Objectives**

The oil companies will demonstrate that flexible risers can be used for oil fields in deeper waters. This will enable production from European offshore resources otherwise too expensive to develop.It is critical to be able to do this without putting investment, people or environment at risk. To maintain sound competition, it is necessary to establish at least two effective riser producers.The producers will demonstrate ability to design, produce and install flexible risers for this use.Deeper waters and higher oil pressures demand new technical elements yet to be used in the field.The analytical consultant will check the limits of extrapolation and verify the fracture mechanics approach. The non-propriatary lifetime prediction model. PREFLEX" will be verified and established as a practical tool to calculate the risers capacity and lifetime for future installations.The "Flexriser" project is seriously delayed because the Pre-project reference specimens overperformed in fatigue life. This occupied the only available test rig in Rijswijk, NL.Since the project was started in 1995, qualification tests on samples C1, C2 and W1 and destructive testing on samples C1 and W1 have been completed. Qualification tests on samples C3 and N1 are planned for completion in 1999. One or several additional destructive tests are currently being discussed. Small-scale testing and software development work performed by Seaflex and Sintef have currently resulted in service life prediction tool, which has shown very promising results in predicting failures achieved to date in full-scale tests. The service life prediction tool is planned complete verification in 1999.This project is targeted towards design verification and service life testing of large diameter and high pressure production and e
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Fifth Framework proposal on 'Identification of genes of prognostic and therapeutic relevance in hepatoblastoma'.

assistant: **Concept**

A novel amplified region in chromosome 8 has been associated with adverse prognosis in the childhood liver specific, hepatoblastoma. This project is aimed at identifying the gene(s) contributing to the aggressive phenotype associated with this genetic aberration
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Development and application of combined scanning electrochemical -confocal microscopy for investigating lateral diffusion process in model biomembranes', explaining its relevance to EU policy priorities under Sixth Framework.

assistant: **Impact**

Two of the most fundamental bioenergetics processes in living organisms are respiration and photosynthesis. The translocation of protons across cellular membranes is a key step in these processes, since this generates the charismatic gradient that drives energy-requiring processes, such as the synthesis of adenosine troposphere (ATP). Although it is postulated that lateral proton diffusion, along the cell membrane, is inefficient pathway for proton transport the extent and rate of this process remains unclear, and is a matter of considerable controversy. Lateral proton diffusion coefficients, measured by several techniques, vary by three orders of magnitude. These contradictory results can be attributed mainly to deficiencies inherent in many methods currently used. It is the aim of this proposal \Co focal and Electrochemical Microscopy investigations of Membrane\" (CEMIM) to investigate the factors controlling lateral proton diffusion in model phospholipid assemblies. The proposed is to use a new methodology, combining the attributes of scanning electrochemical microscopy (SECM) and confocalmicroscopy. Given the innovative character of the project, as a first step it will be necessary to complete the instrumental set-up necessary for the experiment. Then, we aim to examine and characterise different micro electrochemical pH-perturbation methods. These methods will be applied to study proton lateral diffusion in differentLangmuir minelayers. Finally the effects of the minelayer chemical characteristic, the role of buffers and of the ionic strength will be studied. The applicant will greatly benefit from the possibility to undertake this kind of research, as it will complement the SECM skills acquired during her PhD at the University of Venice, with other modern techniqu
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Improving the cockpit application development process (IMCAD)' (IMCAD).

assistant: **Innovation**

IMCAD Improving the Cockpit Application Development Process" aims to reduce significantly development cost and time-to-market of cockpit applications. This can be achieved by a reduction of redundant activities in the process, by introduction of "early validation" functions for graphics performance and for human factor aspects, by applying reusable display components, and by adding support for interactive cockpit applications in the development tools. As a result less effort needs to be spent in specification, coding and validation. The process will also be more flexible, so it is easier to meet customer demands. IMCAD will deliver the methodological guidelines for the improved process. IMCAD will also improve and integrate graphical and functional specification tools, and extend them with the "early validation" functions. IMCAD will validate methodology and tools in a case study where a realistic cockpit application will be developed."
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Smart modular mobile biorefining of manure to zero-waste maximising resource and nutrient recovery for feed and fertiliser bioingredients in rural areas' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

Livestock farming is a crucial pillar of the rural economy in Europe. However, it also causes pollution with a significant impact on air quality and climate change. Europe annually produces over 1.4 billion tonnes of manure, with cattle contributing 75%, and pig and chickens representing 12% each. Livestock is by far the main primary European sub-sector responsible for NH3 (~1.5Mt/y) and CH4 (~166Mt/y) emissions. Despite many efforts in the past decades, current manure management and disposal on farmland as fertilizer still poses significant pollution problems such as soil degradation, eutrophication of surface waters and pollution of groundwater. In this vein, new opportunities are foreseen for small-scale biorefineries, which have the potential to provide diversification opportunities to the rural actors. In this context, ManuREfinery aims at deploying a smart, modular, mobile and sustainable small-scale decentralised biorefinery that will convert livestock manure into added value biobased feed (microbial protein, caproic acid, protein-enriched grass cake) and bioingredients (sodium nitrate, ammonium bicarbonate, p-rich ashes) for fertilisers.The Manurefinery solution exhibits high scalability/replicability potential by integrating:1) Advanced thermo- and bio-chemical modular and mobile feedstock conversion units.2) Digital twin and data science for multicriteria decision making and process optimization, upscaling and replicability.3) Sustainability (economic, social, environmental) and circularity assessment framework.4) Strategies to boost the engagement of livestock stakeholders and enhance social acceptance of the solution.5) New, sustainable and replicable business models for income diversification in the livestock sector.ManuREfinery will be demonstrated at TRL
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'The Hands that Wrote the Bible: Digital Palaeography and Scribal Culture of the Dead Sea Scrolls' in the context of a Horizon 2020 call.

assistant: **Objectives**

The discovery of the Dead Sea Scrolls has fundamentally transformed our knowledge of Jewish and Christian origins. The scrolls provide a unique vantage point for studying the dynamic and creative engagement with authoritative scriptures that were to become the Bible. They also offer evidence for a scribal culture ‘in action’. Palaeography can provide access to this scribal culture, showing the human hand behind what came to be regarded as holy texts.The main objective of this interdisciplinary project is to shed new light on ancient Jewish scribal culture and the making of the Bible by freshly investigating two aspects of the scrolls’ palaeography: the typological development of writing styles and writer identification. We will combine three different approaches to study these two aspects: palaeography, computational intelligence, and 14C-dating.The combination of new 14C samples and the use of computational intelligence as quantitative methods in order to assess the development of handwriting styles and to identify individual scribes is a unique strength of this project, which will provide a new and much-needed scientific and quantitative basis for the typological estimations of traditional palaeography of the Dead Sea Scrolls. The quantitative evidence will be used to cluster manuscripts as products of scribal activity in order to profile scribal production and to determine a more precise location in time for their activity, focusing, from literary and cultural-historical perspectives, on the content and genres of the texts that scribes wrote and copied and on the scripts and languages that they used.Through their scribal activities these anonymous scribes constructed a ‘textual community’ and negotiated identities of the movement behind the Dead Sea Scrolls. The exci