RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Folding Pathways of DNA G-quadruplexes in Crowding Conditions, and Implications for Mass Spectrometry-based Ligand Screening Assays' to be submitted under Horizon 2020.

assistant: **Abstract**

Nucleic acids come in many forms aside from the Watson-Crick duplex. For example, guanine-rich DNA strands can form G-quadruplex structures, which have become attractive targets for small molecule ligands. It is now proven that G-quadruplexes can form in cells. Today most biophysical and structural studies on G-quadruplex folding are carried out in dilute aqueous solutions, but recent works suggest that the folding of some G-quadruplexes may differ in a crowded environment such as the cell environment. In the same way, most in vitro ligand binding assays are today carried out in dilute aqueous solutions. The aim of the project is to assess whether and how the folding pathways of G-quadruplexes differs in dilute and crowded conditions, and whether the community should revise the experimental design of ligand screening assays. We will focus on mass spectrometry-based assays, which have the unique advantage to give a direct read-out of ligand binding stoichiometry, quantity, and the variety of structural ensembles hiding behind the free and bound nucleic acid. The research program involves (1) developing mass spectrometry in more “native” conditions in the sense that we will add co-solutes to mimic cellular crowding, (2) comparing the G-quadruplex folding pathways in dilute and crowded conditions, by combining for the first time mass spectrometry, nuclear magnetic resonance, and single-molecule FRET (Förster resonance energy transfer), and (3) evaluating the impact of crowding on both traditional melting assays and mass spectrometry-based ligand screening assays. Our project will contribute to unveil fundamental principles of nucleic acid folding. It will also foster collaboration between three European institutes specialized in complementary biophysical and structural app
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'A feasibility study to develop local and regional use of wind energy on the Kola Peninsula, Murmansk region, Russia' to be submitted under Fourth Framework.

assistant: **Abstract**

Objectives The objective is to form a basis and to build the necessary tools for substantial integration of wind energy into the energy supply system of the Kola Peninsula. The work will consist of verifying the wind potential at the Kola Peninsula and to establish a Wind Atlas, to dimension wind based autonomous power supply systems for remote areas to assess the possibilities for large-scale integration and the economics of wind integration and, to assess and stimulate a regional interest for wind energy, both on a political and a company level. Technical Appreach This feasibility study has a broad multi-disciplinary view and takes social, technical and economical problems into account. It is divided into two parts, where the first one essentially focuses on reviewing the material produced on the topic and the second more detailed part transforms it into a form that can be a basis for a large scale utilisation of wind energy in the region. During the first phase, preliminary studies on the possibilities for wind energy utilisation in the Kola Peninsula will be made. The study will focus on some key-questions on the basic possibilities for wind energy projects, i.e. wind resource, applicability of current technology, reimbursement by utilities and general regional interest. It will also clarify the need of new information, such as wind measurements at specific sites. The second phase will then consist of more clearly defined work packages, which are more detailed and produce models, tools and detailed studies that are necessary when planning large scale utilisation. In the second phase necessary complementary information, will be produced. Expected Achievements and Exploitation The use of wind energy could stimulate the economic life of the region and have a significan
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'BIOMETAL DEMONSTRATION PLANT FOR THE BIOLOGICAL REHABILITATION OF METAL BEARING-WASTEWATERS' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

Heavy metal pollution is one of the most important environmental problems today even threatening human life. A large number of industries produce and discharge wastes containing different heavy metals into the environment and do not comply with current EU directives. BIOMETAL DEMO project aims at demonstrating the feasibility of the application of novel biotechnologies for the treatment of metal polluted wastewaters though the development of two pilot plants to be implemented in two metal polluting representative industries which are a mine and an electroplating company.Actual metal treatment technologies implemented in polluting industries fail to comply with the reduction of metal concentration required by the EU for wastewater treatments. For this reason, new technologies will be developed to overcome this problem.The biotechnologies that will be evaluated in BIOMETAL DEMO project will be: metal bioprecipitation by sulphate-reducing bacteria and immobilized phytase biocatalysis, and metal biosorption on agricultural industry by-products and biopolymers such as alginate & chitosan based materials.After the evaluation of these techniques, an optimized bioprocess or a synergy of two integrated bioprocesses will be selected to design and build two demonstration pilot plants for scaling-up the metal removal biotreatment.The feasibility of the application of the selected bioprocess will be explored at pilot plant scale in acid mine drainage and electroplating wastewaters contaminated with heavy metals.The operation will be monitored and optimized, and the kinetics and the performance of the metal removal/recovery bioprocess will be integrated within the pilot demonstration plants. Finally, an economic, social and technical analysis of the benefits of such tertiary biotreat
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Analysis of the negative elements controlling Lunatic fringe cyclic expression in the presomitic mesodem' under Sixth Framework.

assistant: **Concept**

Somitogenesis has been shown to involve a molecular oscillator called the \segmentation clock\", which acts in prismatic cells. This molecular clock controls the periodic expression of \"cyclic genes\", which are, so far, all related to the Notch pathway. In the mouse, the cyclic genes include downstream targets of Notch such as those encoding the transcription factors, Hes1 and Hes7 (hairy homologues), and lunatic fringe (Long) that encode aglycosyl transferees, which modulates Notch activity. Recently, distinct cist-acting regulatory elements have been described in the 2.3 kb region in the Lfngpromoter that are required for enhancing and repressing cyclic Long transcription. Analysis of this promoter revealed that the Notch signalling acts directly via CBF1/RBP-ÿK to activate Long expression, and this result is supported by other lines of evidence such as the disruption of Long expression in Notch pathway mutants. Lassies known about how Long is repressed, although this aspect of the control must also be essential for cycling. Therefore, I will focus my analysis on the conserved repressing element, also called box C, that appears crucial to prevent continued expression in PSM and thus to underlie the cyclic Long transcription In order to identify more precisely the negative regulatory elements required for periodic Long expression, we will test the activity in transgenic mice of muleteers of the box C (native and mutated) fused upstream of a non-cycling promoter constitutively active in the PSM. Foot printing of the box C will be a complementary approach towards identification of the transcription factors controlling the cyclic Long repression. We will also try to make cell line derived from prismatic mesoderm using transgenic mice harbouring the thermo labile SV40 Ta
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Quantum Entanglement in a Multiple Quantum Bit Solid State Circuit' will create measurable scientific, societal and economic impact aligned with Sixth Framework.

assistant: **Impact**

The Marie Curie Intra-European Fellowship makes possible a one-year visit by Dr Philip Meson (University of Bristol, U.K.) to the Quatrains Group of Dr Daniel Steve (CEA-Sac lay, France). Dr Meson is an experienced researcher in the field of fundamental physics of superconductivity and most aspects of ultra low temperature technology. He also has some research experience in macroscopic devices. Recent research has focussed on the topic of quantum oscillations (the de Has-van Alp hen effect) in superconductors. He is a co-author of a recent graduate level textbook on low temperature techniques and a permanent member of staff in Bristol. Dr Steve is the head of the Quatrains research group of the CEA-Sac lay. The group is a world leader in Nan fabrication and experimental aspects of the fundamental properties of Nan scale metals and superconductors. Most recently he has been responsible for the landmark development of the ' Quantronium' a super conducting Nan electronic device which has greatly furthered knowledge of macroscopic quantum mechanics and which may form the basis of a future quantum computing technology. The purpose of the visit is to exchange knowledge and promote scientific excellence in the areas of super conducting Nan technology and quantum quits. One outcome of the research in Sac lay will be to engineer the worlds first entangled multiple solid state quit operating with single shot readout. Such an accomplishment is an obvious next step in the development of solid-state quantum computing but the proposal is highly ambitious and requires the implementation of a number of difficult experimental conditions. A principal aim of this work is the transfer of knowledge of Nan fabrication techniques from Sac lay to a new research effort in Bristol to explore fun
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'protein archeology : reconstructed ancestors for protein engineering and crystallography' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

Advances in molecular evolution led to the novel concept of ancestral resurrections, or reconstructions. These allow the exploration of the mechanisms by which new protein families diverged. Reconstructed ancestors are also inherently more prone to functional divergence, and exhibit higher thermal stability, making them more tolerant to mutations, and potentially more evolvable. However, to date, very few ancestors have been resurrected, and the experimental methodologies are still lacking. I aim to develop and apply ancestor resurrections techniques. My first model will be the serum paraoxonase (PON) family. Widespread in vertebrates and bacteria, PONs’ physiological roles span from preventing atherosclerosis to bacterial quorum quenching. Going backwards into PON’s history, we will reconstruct PON’s last common vertebrate ancestor, and acquire insights regarding the divergence of various PON families. The ancestor’s properties (enzymatic and structural) may inform us, for example, how vertebrate PONs emerged from bacterial PONs that acted in quorum quenching. The ancestor, stable and soluble, would also provide an ideal starting point for directed evolution and structural determination. Once the methodologies are developed, other proteins will be subjected to a similar study with the aim of turning ancestor resurrections into a general and powerful tool for protein engineering and structural determination.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Global characterization of post-transcriptional regulatory interactions in DNA damage response' (PostScribeDNAdamage).

assistant: **Concept**

The proposed project aims to identify the dynamics of RNA-protein interactions during DNA damage response (DDR) using high-throughput “omics” approaches.The mechanistic basis of DDR has been classically thought to consist of an ""early"" post-translational response, which results in activation of several signalling pathways and transciption factors. These activate the ""late"" transcriptional reprogramming influencing DNA repair, cell cycle regulation and apoptosis. However, for an immediate and effective response, global post-transcriptional control of mRNA stability, splicing and translation may be crucial during “immediate-to-early” DDR.In this project, I will employ biochemical, cell, systems biology and computational approaches to study the relevance of post-transcriptional regulators such as RNA-binding proteins and microRNAs in DDR. Quantitative proteomics will be applied to identify the differentially mRNA-bound proteins after the exposure of human cultured cells to ionizing radiation. Validation of protein candidates will be carried out by biochemical and deep sequencing approaches. In addition, I will characterize the transcriptome-wide response of existing RNA molecules as well as the newly synthesized transcripts. This will provide a global insight into the dynamics of RNAs and proteins during DDR.The proposed project explores a previously uncharacterized link between post-transcriptional RNA operons and DDR. The results and implementations of this project will significantly contribute to the understanding of the regulation of DDR, as well as have potential clinical and translational impact. Novel tumour-specific RNA-protein interacting partners may serve as candidates to selectively induce cell death in tumour cells.The proposed project will enable a focuse
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Inexhaustible Spring of Hyperpolarization For Magnetic Resonance' (HypFlow), addressing dissemination and exploitation.

assistant: **Impact**

Nuclear magnetic resonance (NMR) has become a well-established and versatile tool in numerous fields of research and in industry, but features a relatively low sensitivity which prevents solving today’s most pressing challenges in modern science.Hyperpolarization by dissolution dynamic nuclear polarization (dDNP) provides a partial solution by enhancing sensitivity 10’000-fold. However, hyperpolarized solutions are available only once (single-shot), and are contaminated and diluted. This is incompatible with most NMR experiments, except for some niche applications.HypFlow will reach the ground-breaking overall aim of providing inexhaustible (multi-shot) and pure (unpolluted and undiluted) hyperpolarization, thus truly compatible with NMR by:1)designing and building a pulsed-DNP freeze&flow polarizer,2) integrating the use of hyperpolarizing matrices with polarized electrons, and3) validating multi-scan applications in metabolomics, drug discovery, and chemistry.Liquid NMR samples will freeze in the HypFlow system where high levels of polarization will be generated, will then melt and flow toward the spectrometer, and will recirculate repeatedly. This will offer, for the first-time, inexhaustible and pure hyperpolarization with a 10’000-fold boost in sensitivity.The PI has a unique combined leading expertise in most recent instrumental, methodological and chemical developments in dDNP that will empower him to tackle the scientific challenges of HypFlow.HypFlow will deepen the fundamental scientific knowledge of nuclear and electron spin polarization generation, transfer, and relaxation phenomena. It will enable detection of hyperpolarized samples in a fully compatible way with a broad range of NMR applications, radically transforming and democratizing the practice of hyp
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Implementation and Sustainability of Microbial Resource Research Infrastructure for 21st Century' (2014-2020) targeting Horizon 2020.

assistant: **Abstract**

IS_MIRRI21 aims to implement the Microbial Resource Research Infrastructure (MIRRI) and secure its long-term sustainability. With this in mind, the mission of IS_MIRRI21 is to serve Bioscience and Bioindustry by providing (a) a broad range of high-quality biological resources and associated data, (b) long-term sustainability of microbial biodiversity, and (c) knowledge and professional development. This mission will be achieved by offering users from academia, governmental laboratories and the private sector access to a portfolio of microbial Biological Resource Centres´ (mBRC’s), services, expertise, education and training build-up and synergistically on the top of mBRC’s proficiencies. To accelerate innovative research processes in life sciences and biotechnology using microorganisms, or their derivatives, IS_MIRRI21 will set up a single-entry point portal (Collaborative Work Environmental) to promote its services, resources, expertise and knowledge transfer activities, as well as to provide access to partners´ resource associated data made interoperable for data searching and data mining. The continuously increasing knowledge will be transferred to users via expert clusters (e.g., for legal framework, for biosecurity, to support training and education programmes, information and cut edge technology, etc.). It will promote cross-disciplinary and innovative solutions (industry and academic) research collaboration through the transnational access offer (pilot study) in different microbial topics defined in pipeline services. With this project IS_MIRRI21 envisages to be a sound partner of the ESFRI landscape on the Biological and Medical Sciences Research Infrastructures, consolidate the operation of MIRRI and enlarge the membership and European coverage and beyond.


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system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'An integrated toolbox for improved verification and prevention of adulterations and non-compliances in organic and geographical indications food supply chain' to be submitted under Horizon Europe.

assistant: **Abstract**

THEROS aims to implement an integrated toolbox being capable to modernise the process of verifying organic and geographical indications food products and preventing adulterations and non-compliances, through the use of various technological innovations and data sources and while demonstrating enhanced security, transparency and interoperability in the quality labelled food supply chain. The envisioned approach consists of : i) low-cost, digital and scalable solutions that rely on Earth Observation, photonics, Internet of Things and DNA authenticity methods being coupled with advanced analytics, machine learning and artificial intelligence in order to ensure efficient detection of fraudulent cases as well as monitoring of associated quality and sustainability elements ii) blockchain enhanced traceability system and dynamic digital product passport for improved traceability, security and transparent data governance iii) platforms and algorithms allowing management and harmonisation of heterogenous data as well as their consolidation for the verification and validation of transactions across the supply chain iv) interfaces to facilitate monitoring and inspections by competent authorities, informed decision making by supply chain actors and policy makers as well as business model driven approaches to support short supply chains of quality labelled food products. THEROS toolbox components will be extensively evaluated in real life settings through four pilot demonstrations in four different countries and while covering diverse requirements, involvement of all relevant actors and different organic/geographical indication food products and fraud/adulteration cases.


**Keywords:** organic and geographical indications supply chain, Earth Observation, photonics, machine learning