RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon Europe proposal on 'Tracing and harnessing the ancestry of RNA-guided nucleases'.

assistant: **Concept**

The phylogenetic, biochemical and functional diversity of these linked RGNs suggest a common origin that gave rise to the innumerable variations we observe today. However, little is known about these ancestors and the evolutionary paths that led to their modern counterparts. Recently, our team showed that reconstructed ancestors of Cas9 and Cas12a RGNs exhibit flexible substrate recognition and collateral activities distinct from any modern counterpart. This work sets the stage to uncover the evolutionary trajectories that gave rise to all related RGNs and to translate insights into new and improved biomolecular tools. As part of RGNcestry, we assembled four accomplished PIs covering CRISPR/transposon biology and technologies, ancestral sequence reconstruction and structural biology. The ensuing project will test our working hypothesis that ancestral RGNs consistently exhibit flexible properties that allow them to function in roles paralleling their modern counterparts, albeit less effectively due to a lack of specialization. To test this hypothesis and translate the resulting discoveries, we will pursue four objectives:  1.  Reconstruct a detailed ancestral tree uniting RuvC-harboring RGNs.  2.  Define the properties and functional roles of ancestral RGNs.  3.  Explore molecular features and dynamics of ancestral RGNs.  4.  Enhance and apply ancestral RGNs.If successful, our high-risk/high-reward project could reveal how RGNs evolved into the rich diversity observed today and establish a new paradigm for RGN engineering and application spanning genome editing to molecular diagnostics.


**Keywords:** TXTL, CRISPR, transposon, ancestral reconsruction, structural biology, protein engineering, genome editing, molecular diagnostics
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'A distributed interactive audio-visual virtual reality system' demonstrating excellence, impact and implementation quality for a Sixth Framework call.

assistant: **Abstract**

The goal is to create an open source Internet platform for multi-user, interactive, distributed, high-quality 3D graphics and audio for home, public and personal use. The platform will support high-quality 3D-graphics as well as high-quality 3D-audio and acoustic simulation.The foundation of the platform is a lightweight, low latency, general-purpose network protocol for 3D data, which lets multiple applications act together as one large system by sharing data over the network. If one application makes a change to the data the change is distributed instantly to all interested clients. Therefore rendering engines, tools, simulation engines and other components can be separate applications working together over a network to form an application. The platform will be scalable from simple PDA's to large immersive environments.The core of the project is to integrate state-of-the-art technology from different partners using this protocol. This will form a consistent and useable system with unique dynamic properties The components we intend to develop include an advanced 3D graphics engine, a unique 3D audio acoustics simulator, tools and visual scripting and computing engine as well as integrating new and existing tools. We also intend to build and research new opportunities given this highly dynamic collaborative system such as dynamic mesh generation, adaptive global illumination solutions, distributed computing.Our research will be focused on integration as well as development in the respective fields. We will also research the scalability of the system for future wide spread use. The platform will be tested in two application areas, architecture and digital media. We believe that such a platform could have an impact on the interactive media industry as well as design, arts
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'The last piece of the puzzle: Off-shell hypermultiplets in string theory and complex geometry', explaining its relevance to EU policy priorities under Seventh Framework.

assistant: **Impact**

The hypermultiplet sector in N=2 supersymmetric theories has long defied a complete understanding. However, recent workon the couplings of off-shell hypermultiplets in flat space has opened the door to a comprehensive treatment of their supergravity couplings. In pursuit of this goal, this proposal aims to construct a complete description of off-shell N=2 supergravity-matter actions, including both vector and hypermultiplets. This will (i) shed light on the moduli spaces of low energy effective actions of string theory; (ii) enable computations of hypermultiplet contributions to the thermodynamic properties of supersymmetric black holes; and (iii) allow the efficient application of localization to both the hypermultiplet and vector multiplet sectors. The success of this project will place our understanding of N=2 supersymmetric systems on a firm foundation, while at the same time giving new cross-disciplinary insight into the fields of complex geometry and quantum gravity.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Game changer in high temperature steam electrolysers with novel tubular cells and stacks geometry for pressurized hydrogen production' (GAMER) under Horizon 2020.

assistant: **Objectives**

The GAMER project will develop a novel cost-effective tubular Proton Ceramic Electrolyser (PCE) stack technology integrated in a steam electrolyser system to produce pure dry pressurized hydrogen. The electrolyser system will be thermally coupled to renewable or waste heat sources in industrial plants to achieve higher AC electric efficiency and efficient heat valorisation by the integrated processes. The project will establish high volume production of the novel tubular proton conducting ceramic cells. The cells will be qualified for pressurized steam electrolysis operation at intermediate temperature (500-700°C). They will be bundled in innovative single engineering units (SEU) encased in tubular steel shells, a modular technology, amenable to various industrial scales. GAMER will develop designs of system and balance of plant components supported by advanced modelling and simulation work, flowsheets of integrated processes, combined with robust engineering routes for demonstrating efficient thermal and electrical integration in a 10 kW electrolyser system delivering pure hydrogen at minimum 30 bars outlet pressure. The consortium covers the full value chain of the hydrogen economy, from cell and SEU manufacturer (CMS), system integrators (MC2, CRI), through researchers (SINTEF, UiO, CSIC), to end users in refineries, oil and gas, chemical industry (CRI, Shell with advisory board members YARA and AirLiquide). All along the project, these experienced partners will pay particular attention to risk management (technical, economic, logistic, business) and ensure progress of the technology from TRL3 to TRL5. The overall consortium will perform strategic communication with the relevant stakeholders in order to ensure strong exploitation of the project’s results.


**Keyword
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Evaluation of diphtheria - and tetanus-toxin neutralizing anibody activity in human and animal sera' (1994-1998).

assistant: **Concept**

* To investigate the nature of the reported absence of correlation between in vitro toxin neutralization assays and in vivo neutralization tests for diphtheria (and to a lesser extent tetanus) antibodies and to develop a practical solution for this phenomenon.* To improve research and development capacity in vaccine quality control laboratories of public sector vaccine producers in two developing countries (Indonesia and Vietnam). * To ensure quality in immunization programmes and local vaccine production by developing and making available scientifically validated test systems that can be used in developing countries.Expected Outcome* Understanding of the factors which have an effect on the mechanism of toxin neutralization.* Recommendations to regulatory authorities on the selection and standardization of in vitro toxin neutralization tests.* Research training to 2 Bio Farma and 2 IVAC staff workers for a period of 4 months each.* The organization of a workshop on the immunological aspects of diphtheria toxin neutralization and antiserum production. * Improved horse immunization schedules and the introduction of plasmaphoresis for the production of anti-D serum.* Establisment of anti-diphtheria serumpanels from 4 different species* Study of kinetics of neutralization test systems for diphtheria in in vitro tests (Vero cell test, ToBI, Double antigen ELISA, DELFIA) and in vivo tests (skin test in guinea pigs) using high, intermediate and low doses of toxin.* Standardization of one or two selected in vitro toxin neutralization test systems, with established validity for the in vivo neutralization mechanism.* Improve horse hyper-immunization schedules and introduction of plasmaphoresis.* Provide research training to staff at RIVM (two from Vietnam and two from Indonesia)
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Geometry and Topology of Singularities', explaining its relevance to EU policy priorities under Horizon Europe.

assistant: **Impact**

This interdisciplinary project brings together innovative ideas and techniques from algebraic geometry and complex analytic geometry, singularity theory, commutative algebra and topology. Given two complex analytic varieties X and X' that are part of an analytic family in a complex affine space we will study the relationships between the different similarities X and X' might share. Such similarities can be of geometric and topological nature.The synonym for similarity we will use here is equisingularity. We say X and X' are topologically equisingular if there is a homeomorphism between X and X' that extends to an ambient homomorphism that trivializes the entire family; we say that X and X' are geometrically equisingular if X and X' have similar resolution of singularities, or if a resolution of the entire family induces a resolution of each fiber. Understanding the topology of either X or X' usually involves a continuous deformation of either one of them to a smooth variety, the existence of which is guaranteed under a special smoothability hypothesis.  A stronger version of topological equisingularity, called Lipschitz equisingularity, asks for a homeomorphism between X and X' that satisfies the Lipschitz inequality. The ultimate goal of the project is to gain insight in the relationships between topological (Lipschitz) and geometric equisingularity  and develop an approach to study the topology of singularities that are not necessarily smoothable without imposing special dimension (or codimension) assumptions as was done previously.


**Keywords:** singularities, equisingularity theory, resolution of singularities, multiplicities, integral closure, Lipschitz geometry
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Bioactive multiphase membrane for periodontal regeneration', explaining its relevance to EU policy priorities under Horizon Europe.

assistant: **Impact**

Periodontitis is one of the world's most prevalent chronic dental and maxillofacial diseases that will ultimately lead to unwanted tooth loss. This disease was responsible for a significant loss of  158.64 billion in Europe in 2018. Due to the complexity of the grafting treatment techniques, the efficiency of these procedures is notably affected. A bioactive multiphasic membrane/scaffold with different layers has been advised for this. Until now, few commercial companies using synthetic polymers like polylactic acid (PLA), and woven fibers made up of polyglactin 910 copolymer together with collagen have developed this type of membrane/scaffold. However, different limitations of these materials make their application difficult. MultiphaseGTR focuses on the preparation of bioactive multiphase hydrogel-based biomembrane for periodontal regeneration. The fundamental objectives of the study are: I. Development and characterization of the two components of the biomembrane, i.e., non-mineralized hydrogel and mineralized hydrogel membranes (WP1); II.  Preparation of a multiphase hydrogel membrane by combining the two components, i.e., non-mineralized and mineralized hydrogel membranes, and characterization of their physicomechanical properties (WP2); and III. Assessment of the bioactive properties of the multiphase biomembranes in relation to periodontal regeneration (WP3). Finally, a suitable multiphase membrane will be recommended. The outcome of this proposed work not only intends to fill the current gap for the requirement of a suitable cost-effective alternative to the current limited reported materials but also to elaborate the opportunity to increase the knowledge regarding the utilization of biomembrane in dental and maxillofacial tissue regeneration.


**Keywords:** gu
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'OpenFlow City Experiment – Linking Infrastructure and Applications' under Seventh Framework.

assistant: **Concept**

This Future Internet experiment will create a multi-autonomous-system experimental signalling overlay on the OFELIA OpenFlow infrastructure. We propose to marry an existing NSIS signalling stack, along with an existing multi autonomous system Virtual Path Slice controller, to the OFELIA OpenFlow experimental facility, thereby extending OpenFlow with signalling and multi-domain  interworking on a multi-autonomous-system topology. We will use the Virtual Wall facility to emulate a city broadband network with up to 100 nodes, and will model the network based on best practices as observed in public internet production networks.<br/>The work will be conducted in three steps (i) experiment setup and configuration (ii) experiment implementation and (iii) showcase and demonstration.<br/>Altogether four experiments will be performed. The first experiment will verify the inter-working of NSIS and the VPS Controller with OpenFlow. The second experiment concerns signalling performance; the intention is to measure the performance of the control plane with high signalling load and identify what Busy Hour Connection Attempt (BHCA) performance can be achieved. The third experiment will measure the resilience of the virtual path slice in the context of high background traffic load for uni-directional and bi-directional traffic. Finally, another experiment will investigate how the system reacts under different failure scenarios.<br/>The showcase will demonstrate the benefit of signalling for two use cases: (a) a scenario for true HD video; and (b) a HD video-to-video scenario for real-time interactive traffic.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Applying Reactive Twins to Enhance Monument Information Systems' under Horizon Europe.

assistant: **Concept**

The ARTEMIS proposal addresses the field of cultural heritage conservation, restoration, safeguard and valorisation by advanced digital technologies, using digital twins and relying on advanced AR/VR technologies. It relies on an extended digital documentation of heritage assets with 3D models incorporated in their digital twins, by implementing services on heritage data that model the behaviour of the real-world assets in different conditions and under the effect of complex external phenomena. The same approach will allow digital experiments testing the effect of activities simulated on the twin. Whenever suitable, the developed services will avail of AR/VR techniques to manage the simulation input and to visualize the outcomes. ARTEMIS will rely on the know-how of participating world-class research infrastructures, making available a huge amount of heritage documentation data, on the contribution of advanced research centres and on the support of specialized technological SMEs. The project will produce a scientific methodology tested on a large number of pilots and will pave the way for innovative applications in heritage research based on digital twins and AR/VR techniques.


**Keywords:** digital twins, cultural heritage, conservation, safeguard
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Understanding and modifying cell-fate transitions during plant grafting' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

The remarkable regenerative capacity of plants to join wounded tissues together through a process known as grafting has been extensively used in agriculture to combine the best properties of two different plants into a single plant. Although grafting has been practiced for thousands of years, many agriculturally important plants are not possible to graft. One of the major limitations that prevent us from improving grafting efficiency is our poor understanding of the molecular mechanism of plant regeneration. When tissues are wounded and joined for grafting, the differentiated cells near the wound site modify their identity and acquire a dedifferentiated fate. These pluripotent cells then differentiate to form the missing cell types leading to tissue regeneration. However, it remains unknown how cells dedifferentiate in response to wounding and how they differentiate to heal tissues, the major problem to be addressed in this project. This gap in knowledge will be addressed by using multidisciplinary approaches that include my experience and skills in plant regeneration, molecular genetics and advanced microscopy, and the host lab's expertise in plant grafting, transcriptomics, and genetics of various plant species. The outcome of this research is expected to enhance the fundamental knowledge in cell-fate transition and vascular regeneration and provide powerful tools for improving grafting in various plant species. The project will allow me to be trained as an independent researcher in plant grafting and vascular regeneration. At the same time, the host lab will benefit from my expertise in plant regeneration and microscopy.


**Keywords:** Grafting, cell-fate transition, regeneration, dedifferentiation, differentiation, scion, root-stock
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'multi-hAzard monitoring and earLy wARning systeM' (ALARM).

assistant: **Innovation**

The overall objective of ALARM (“multi-hAzard monitoring and earLy wARning systeM” (ALARM) is to develop a prototype global multi-hazard monitoring and Early Warning  System (EWS). A global multi-hazard monitoring means near-real time (NRT) and continuous global Earth observations from satellite, with the objective to generate prompt alerts of natural hazards affecting ATM and to provide information for enhancing situational awareness and providing resilience in crisis. NRT data (with delay of delivery from 10 min, to less than 4h) and tailored products from ground-based and satellite systems, will be used to feed models capable of detecting (creation of alert products) and predicting (nowcasting/forecasting) the risk/displacement of:•particles in suspension and gas derived from natural hazards (volcanic ash and SO2, dust clouds from sandstorms, and smoke from forest fire);•severe weather situations such as deep convection and extreme weather;•space weather regarding exposure to increased levels of radiation during flight; •environmental hotspots potentially contributing to global warming in a large extent. ALARM EWS plans to provide alert information for the stakeholders about the threat to aviation in three different manners: 1) early warning (geolocation, altitude and level of severity -quantification if available- of the observed hazard); 2) for some cases nowcasting [up to 2h] and 3) forecasting [from 2h to 48h] of hazard evolution at different flight levels. The consortium will analyse the requirements for its inclusion in the yellow SWIM profile. Specifically, the aim is to enhance situational awareness of all stakeholders in case of multiple hazard crisis by facilitating the transfer of required relevant information to end-users, presenting such information in a