RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon Europe proposal on 'Hybrid polymer nanoparticles as gene vectors'.

assistant: **Concept**

Gene therapy has long been seen as a promising solution to unmet clinical needs and the licencing of gene therapy SARS-CoV-2 vaccines has provided a flavour of the technology’s potential. However, gene therapy still faces significant challenges in clinical and commercial translation including the need for effective and safe delivery vectors. Polymeric nanoparticles offer great flexibility in gene vectors design, improved stability and easier scale-up of production compared to lipid nanoparticles but often suffer from cytotoxicity and underperformance in vivo. The goal of HyPlex is to develop the next generation polymeric nanovectors which can easily be functionalised to further improve delivery efficacy. The project will develop novel chemistry to achieve polylysine/polyacrylate hybrid nanogels. Functionalisation of nanogels will be demonstrated by the introduction of mannose, a common cell binding motive as well as diethylene glycol ethyl ether arylate to optimise the hydrophilic/hydrophobic balance. In an interdisciplinary approach gene loading and cell transfection will be studied up to in vitro level. The project results will be exploited as part of an ongoing commercialisation initiative at RCSI, which will provide the applicant with a unique experience in research translation and commercialisation. The high-level science is complemented by bespoke training activities, which will significantly advance the career opportunities of the applicant. Project results will be communicated to peers as well as the public with specific focus on encouraging female secondary school pupils to take up scientific careers.


**Keywords:** nanogels, polypeptides, polyacrylates, gene delivery, soft nanomaterials
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Decrypting signals in the crypt' (DECRYPT), addressing dissemination and exploitation.

assistant: **Impact**

Pathogens and symbionts: War and Peace at mucosal surface in intestinal crypts.In the proposed program called DECRYPT, I wish to strengthen novel orientations of our laboratory aimed at decrypting the dialogue between the microbiota and the host, while keeping a balance with the study of pathogens, both being analyzed at their interface with the gut mucosa to further our knowledge of the homeostatic and pathogenic mechanisms that respectively characterize a healthy and a diseased gut. The intestinal crypt is a key location to study this dialogue because it contains the stem cells, the differentiation and transit amplifying/proliferative compartments that are essential for epithelial regeneration at homeostasis, and restitution following an aggression. It is also embedded in a niche of immune cells that participate in homeostatic and pathological processes under microbial stimuli. Thus the breaking nature of my project will bear on the demonstration that crypt homeostasis depends on signals “emitted” by the microbiota, thereby stressing the depth of our symbiosis with the microbial world, and on the demonstration that the crypt is also the target of enteric pathogens like Shigella, thus introducing the novel paradigm that pathogenesis is not only matter of inflammatory destruction of infected tissues, but also of altered epithelial restitution. An extension of this paradigm is that loss or subversion of the microbiota-crypt homeostasis may account not only for inflammatory bowel diseases (IBD), but also for colon cancer. This fundamental knowledge will also be the basis for translational research, particularly the search for molecules that boost antimicrobial defenses and comfort homeostasis. In summary, I propose a balanced combination between the “cellular microbiology
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'The eco-metabolism of estuarine intertidal flat' (ECOFLAT), addressing dissemination and exploitation.

assistant: **Impact**

The objectives of the study are: * To study processes important for the carbon and nutrient cycling within a tidal flat ecosystem, and between the tidal flat system and the estuary. * Development of predictive mathematical models describing the processes and relating them to the main forcing factors for the tidal flat system. * Bridging the gap between the scales at which the processes operate and are measured, and the ecological target values for environmental management and prediction of the ecological consequences of global change.Tidal flats are important components of macro- and mesotidal estuaries all over the world, yet the mechanistic  description of carbon and nutrient cycling within tidal flats and between tidal flats and the water column is far  from complete. This description requires the multi-disciplinary study of physical, chemical and biological  processes and fluxes at small spatial and temporal scales. In contrast, environmental management of estuarine  and coastal areas requires answers and solutions to problems posed at larger scales (estuarine scale, regional  scale). The project aims at bridging the gap between the smaller scales at which processes operate and are  measured, and the larger scales relevant for management, by explicitly studying the small scale processes and  by integrating them in mathematical models.  The project's work programme is centered around five joint field campaigns by all participants. The first four  of these are executed on a tidal flat in the Westerschelde estuary (S.W. Netherlands). Validation of the models  and predictions will be done during a final campaign in a different estuary.  The research is subdivided into three work packages.  The first package focuses on the quantification and prediction of benthic-pelagic
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Electroweak Baryogenesis in the Era of the LHC' (EWBGANDLHC).

assistant: **Innovation**

The recent discovery of the Higgs boson at the Large Hadron Collider (LHC) constitutes a milestone in a major work programme to test the Standard Model and search for new physics, in order to obtain a global picture of electroweak symmetry breaking and its possible connections to open questions in particle cosmology. A key question relating the high-energy, precision and cosmic frontiers in particle physics is the origin of the cosmic matter-antimatter asymmetry. The present proposal will combine for the first time experimental and theoretical inputs from all three frontiers  in order to test one of the most compelling ways to explain the generation of this asymmetry in the early Universe: Electroweak Baryogenesis. Together with leading scientists from the Physics Department in the University of Sussex, which hold a unique expertise at the interface of these frontiers, I will design and develop a comprehensive strategy to search for viable Electroweak Baryogenesis scenarios at LHC, in combination with present and future experimental inputs from neutron/electron electric dipole moments (e.g. from Sussex/RAL Cryo-EDM experiment) and detection prospects from the foreseen gravitational wave mission eLISA/NGO from the ESA.A dedicated LHC search strategy aimed at probing new physics scenarios where the matter-antimatter asymmetry could be generated is at present essentially absent from the main LHC physics programme. The project will make this analysis available for the ATLAS and CMS experimental collaborations, and aims to trigger interest towards these searches in their community, taking advantage of the numerous high-quality national/international collaborations and networking opportunities (exemplified by SEPNet and NExT Institute consortiums) provided by the host. This w
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Fifth Framework proposal on 'Molecular cloning and characterization of the membrane-bound progesterone receptor in xenopus oocytes'.

assistant: **Innovation**

Magnus Doverskog has been working as a Ph.D. student at the department of Biotechnology, Royal Institute of Technology, Stockholm, from January 1995 to the dissertation, May 26, 2000, under my supervision. From almost daily contacts during five years, I know Magnus Doverskog very well. Dr. Doverskog has been working on physiology and metabolism of cultured insect cells. Part of the work has been focused on the central metabolism, using NMR techniques for tracing 15N and 13C in metabolites. This work lead to a new view on the metabolism in Spodoptera frugiperda, Sf9, insect cells. Most surprisingly, it was shown that the nitrogen fixation system is present in these cells. This system was earlier thought to be present only in microorganisms and plants. Another part of Dr. Doverskog's work has dealt with amino acid metabolism, and in particular that of cystine/cysteine. All cultured insect cells had been reported to require cystine in the medium for growth. Dr. Doverskog could show that Sf9 cells were able to synthesise cysteine via the cystathionine pathway, provided that the cells were in a particular physiological state. This work lead to an investigation of the occurrence of autocrine growth factors and hormone systems in Sf9 cells. An insulin binding protein was identified in culture supernatants of Sf9 cells. This was the first report of such a protein in insect cells. A small peptide required for initiation of mitosis was also detected in the culture supernatant. In addition, the loss of productivity occurring at high cell densities in the baculovirus expression system could be related to auto-regulatory events controlling cell cycle progression. These findings will have a great impact for developing new control strategies for the baculovirus expression system. The
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Sixth Framework proposal for the project 'Farm animal cloning and the public; a project to facilitate a European public debate and to make recommendations on regulation and on guidelines for research and applications of farm animal cloning'.

assistant: **Objectives**

The aims of CLONING IN PUBLIC are (a) to develop recommendations for European regulation and for guidelines on research regarding the use of farm animal cloning such as genetically modified animals for bio-reactors, and (b) to foster an informed public, European debate on these issues, including major stakeholders, university students and representatives of the general public from different parts of Europe.The two aims are of equal importance. They are interrelated because regulation or guidelines, to serve their purpose, must take public concerns into account. Inspiring and informing debate is, however, also a purpose in its own right and with the long term perspective of improving communication between science, civil society and European authorities at different levels on European public affairs related to science and technology.What is the state-of-the-art concerning farm animal cloning? Which objectives and rationales are driving the research in this field? How is animal biotechnology regulated at present? What do we know about public attitudes and ethical concerns? Information relating to these questions will be gathered and synthesized through a combination of literature reviews, individual interviews, focused public workshops and a final, larger participatory conference. The project's focus is on applications of farm animal cloning in agriculture, food production and in biomedical research and development.Experts on different aspects of farm animal cloning will be brought together with representatives of industry and other NGOs and students and citizens' representatives from different parts of Europe in the public workshops and the synthesizing arrangement with an active, participatory element. Together with the background studies and the interviews, these events
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Molecular mechanisms of microvascular dysfunction following hemorrhagic stroke' (MICROFLOW) under Seventh Framework.

assistant: **Objectives**

Stroke kills over 300,000 European citizens each year. Among the various forms of stroke subarachnoid hemorrhage (SAH) has the highest mortality, the worst prognosis, and the least well understood pathophysiology. Recent studies from the applicant's laboratory suggest that disturbances of the cerebral microcirculation may play an important role for brain damage after SAH. So far, however, little to nothing is known about the cellular or molecular mechanisms leading to microvascular dysfunction (mvD) following SAH resulting in an absolute lack of diagnostic and therapeutic options for affected patients. Therefore the aim of the current program is to investigate morphology and function of the cerebral microcirculation after SAH, to determine the molecular mechanisms responsible for mvD, and to use this knowledge to develop novel biomarkers and therapeutic options for SAH patients. For this purpose we will subject mice expressing fluorescent proteins in specific cells of the cerebral microcirculation (endothelium, pericytes, smooth muscle cells) to SAH and assess the temporal profile of morphology and function of these cells in vivo by 2-photon microscopy. To investigate gene expression associated with mvD we will employ microdissection microscopy for the isolation of microvessels from tissue sections and a novel strategy designed to capture mRNAs from specific cell types from whole brain tissue by using the Cre-lox system together with a transgenic mouse expressing an affinity-tagged ribosomal protein (Ribotag technology). Cell specific mRNA will then be analyzed by microarray technology. Finally, a small inhibitory RNA/lentiviral approach will be used to assess the therapeutic potential of identified disease-associated genes.The proposed research program may therefore re
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Glycans coated nanoparticles as a biocompatible shield to increase nanoparticles colloidal stability and modulate their immunological response.' to be submitted under Horizon 2020.

assistant: **Abstract**

Nanomedicine is currently the most studied and promising topics in the field of biomedical technology, which is focused on developing nanoparticles. The reason for this is that these materials present unique properties such as size, high ratio area/ volume, chemical reactivity, that offer potential solutions for many of the current challenges in diagnosis, therapy and vaccines on several diseases. However, the safe use of NPs in clinical applications is not established yet. At present, the NPs have limited stability in complex cell media, enhanced recognition by the immunological system and reduced targeting efficacy as a result of the protein corona formation. Studies in Dr. Monopoli´s laboratory at RSCI have demonstrated that glycosylation of the protein corona plays an important role in maintaining the colloidal stability of nanoparticles and influences nanoparticle-cell interactions. The proposed research programme will utilize the combination of interdisciplinary and intersectoral approaches (synthetic chemistry, physical chemistry, glycoprofiling and proteomics, an inter-sectoral secondment (CIC biomaGUNE, Prof Sergio Moya to learn fluorescence correlation spectroscopy and test the NP targeting efficacy) to synthetise a range of nanoparticles (NPs) decorated with synthetic and naturally occurring glycans (hence, Glyco-NPs) in order to: 1), increase the NPs' colloidal stability, 2) attenuate the unspecific protein interaction and 3) increase the NPs circulation half-life into the bloodstream. Together with my supervisor, we have designed a training plan that includes new research (glycoprofiling and bio-nanointeractions), and transferable (project management, leadership, innovation) skills.


**Keywords:** Nanoparticles, protein corona, glycans, colloidal stability
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Nanostructured Mesoporous Polyoxometalate and Transition Metal-substituted Polyoxometalate Materials: Applications in Heterogeneous Catalysis' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

The synthesis of nanostructured mesoporous polyoxometalates with high internal surface area and ordered or/and hierarchical pore structure remains an open challenge. This proposal aims to the development of such nanostructures. On one hand mesoporous materials with well-defined pore structure and high internal surface area are of great scientific and technological interest because of the possibility of tailoring their pore structure and framework composition. On the other hand metal-oxygen cluster anions of the early transition metals, commonly referred to as polyoxometalates and transition metal-substituted polyoxometalates, are important supramolecular inorganic nanoclusters with wide range of chemical structures and physicochemical properties and, thus attractive for applications such as photocatalysts, redox catalysts, electrochemical sensors and magnetic and optoelectronic devices. It would be very interesting to combine the shape selectivity of a mesoporous solid with the catalytic, optoelectronic and magnetic properties of a polyoxometalate cluster. Polyoxometalate clusters can provide novel building blocks, when they became nanostructured, promising new type of multifunctional nanomaterials We intend to establish new synthetic strategies toward nanostructured polyoxometalate-based mesoporous materials with well-defined pore structure and high internal surface area. For example purely inorganic and organic-supported nanostructured POM-based materials such as SiO2-, TiO2-, Al2O3-, SnO2-POM and {Q}-POM (where (POM= [(Mn,Ni)Mo9O32]6-, [(Co,Zn)PW11O39]5-, [(Eu)W10O36]9- and Q= 4,4-bipyridine, [Ag2(CH3CN)4]2+, (R’O)3SiRSi(OR’)3) will be explored. Integration of these POM nanostructures in thin film morphology will also be attempted. The resulted mesoporous polyoxometa
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'OncoMasTR - Novel Prognostic Assay for Early Stage Breast Cancer' demonstrating excellence, impact and implementation quality for a Horizon 2020 call.

assistant: **Abstract**

OncoMark Ltd are developing a novel, game-changing breast cancer prognostic assay, called OncoMasTR, which has the potential to transform clinical practice and patient care. OncoMasTR will help clinicians to decide which patients should receive chemotherapy for early stage breast cancer, which is a pressing clinical and economic issue worldwide. OncoMasTR is more cost-effective than competitor assays, demonstrates superior performance clinically, generates a user-friendly binary (yes/no) output and can be carried out on-site using standard equipment. Our principal aim is to develop OncoMasTR as a CE marked kit, and penetrate a global market. OncoMasTR comprises a novel panel of biomarkers of tumour progression, measured by qRT-PCR using standard procedures and equipment. Objectives for Phase 2 are to analytically, clinically and commercially validate the OncoMasTR prototype under ISO13485 standards, using two large cohorts of breast cancer patients. Access to tissues from a prospective clinical trial will enable us to benchmark OncoMasTR’s performance against the leading competitor assay. Communication and dissemination of data will be key to building a strong base of OncoMasTR ambassadors in the clinical arena, and to leverage private investment in OncoMark for development of other products in our pipeline. OncoMasTR will bring OncoMark into the clinical biomarker arena and will positively impact healthcare costs in the EU and globally.