RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Development and application of ultra-high resolution nano-organized films by self-assembly of plant-based materials for next generation opto- and bio-electronics' (GREENANOFILMS), addressing dissemination and exploitation.

assistant: **Impact**

Carbohydrate biomass constitutes an abundant and renewable resource that is attracting growing interest as a biomaterial. Convincingly the use of different natural “elementary bricks”, from oligosaccharides to fibers found in biomass, when mimicking self-assembly as Nature does, is a promising field towards innovative nanostructured biomaterials, leading to eco-friendly manufacturing processes of various devices. Indeed, the self-assembly at the nanoscale level of plant-based materials, via an elegant bottom-up approach, allows reaching very high-resolution patterning (sub-10nm) never attained to date by petroleum-based molecules, thus providing them with novel properties.GREENANOFILMS aims to use carbohydrates as “elementary bricks” (glycopolymers, cellulose nanocrystals and nanofibers) for the conception of ultra-high resolution nanostructured technical films to be used in various markets, from large volume sectors, such as (i) high-added value transparent flexible substrate for printed electronic applications, (ii) thin films for high-efficiency organic photovoltaics, to growing markets, such as (iii) next generation nanolithography and (iv) high-sensitivity SERS biosensors.GREENANOFILMS main impacts are the implementation of a new generation of ultra-nanostructured carbohydrate-materials that will play a prominent role in the achievement of the sustainability improvement of various opto- and bio-electronic sectors. A network of industrial end-user leaders is integrated in the project to facilitate the innovator-to-market perspective. The prospective environmental impacts and benefits of new green processes, eco-efficient nanomaterials and nanoproducts will be quantified with Life Cycle Assessment, risk assessment and validation of the industrial feasibility, includi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Chemical ecology of Antarctic opisthobranch molluscs' (1994-1998).

assistant: **Innovation**

Research objectives and content The basic aim of this project is to determine the role that natural products play in the interactions among benthic organisms in Antarctic ecosystems, considering the opisthobranch molluscs as a model. In order to develop this project the following specific objectives are proposed: Objective 1) To isolate, analyze and structurally identify the bioactive chemicals present in selected opisthobranchs, involved in both intra- and interspecific relationships (e.g. prey-predator pairs). Objective 2) To ascertain the origin and localization of the main chemical compounds, coming either from a prey, or by establishing the biosynthetic pathway, on the basis of the nature of the chemicals and the putative trophic relationships. Localization within the body of the producing or accumulating organism, is planned in order to find how are they protected from their own allomones, and to facilitate the development of the next objective. Objective 3) To determine how the molluscs use these metabolites. Experimental tests will focus on antipredatory effects against putative predators and toxicity to other co-habiting organisms. This part includes not only laboratory testing against putative predators from the same habitat, but also in situ field testing, in order to obtain ecologically valuable information. It also includes testing chemical attraction by using Y-maze tests. Training content (objective, benefit and expected impact) Links with industry / industrial relevance (22)
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Parallel network-based biocomputation: technological baseline, scale-up and innovation ecosystem' in the context of a Horizon 2020 call.

assistant: **Objectives**

Many technologically and societally important mathematical problems are intractable for conventional, serial computers. Therefore, a significant need exists for parallel-computing approaches that are capable to solve such problems within reasonable time frames. Recently, part of our consortium demonstrated proof-of-principle of a parallel-computation system in which a given combinatorial problem is encoded into a graphical, modular network that is embedded in a nanofabricated planar device. The problem is then solved by a large number of independent biological agents, namely molecular-motor-propelled protein filaments, exploring the network in a highly parallel fashion (PNAS 113, 2591 (2016)). Notably, this approach uses orders of magnitude less energy than conventional computers, thus addressing issues related to power-consumption and heat-dissipation. Within Bio4Comp we (i) will establish the technological and scientific basis for robust upscaling of this approach, (ii) will demonstrate scalability by systematically increasing the problem size by several orders of magnitude, and (iii) will develop new algorithms with the aim to open up a wide range of applications. Additionally, we will (iv) help foster and structure an ecosystem of scientists and companies that will accelerate the path to market acceptance, including the creation of a joint roadmap. Benefits to society will include the ability to solve hitherto intractable problems, and the development of a sustainable and energy-efficient computing approach that is radically different from current information and communications technology.


**Keywords:** Parallel computing, alternate computing paradigm, molecular motors, biocomputation, nanotechnology, innovation ecosystem
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Small-scale combined heat and power (CHP) from bio-crude oil fuelled to a stirling engine' (CHP).

assistant: **Innovation**

OBJECTIVES OF THE PROJECT The main objectives of this project include: - the development of feedstock logistics for BCO production via fast pyrolysis - the scale-up potential of biomass fast pyrolysis will be clearly deducted - the production of BCO for fuelling a Stirling engine for CHP generation - the development of a suitable burner to fuel BCO and further adaptations in an existing Stirling engine - the techno-economics of the technology including Life Cycle Assessment (LCA) the market studies for the penetration of both fast pyrolysis technology and end-use applications of BCO. TECHNICAL APPROACH Renewable energies, and in particular biomass, are among the most suitable options to gradually replace conventional energy sources and stabilise CO2 emissions related to electricity and heat generation. A liquid fuel derived from solid biomass by fast pyrolysis (Bio-Crude-Oil or BCO), will be of great interest for the fuelling of efficient, in the small-scale (<150 kWe) engines for Combined Heat and Power (CHP) production. The project will consist of 6 main tasks, namely: Task 0: Project co-ordination Task 1: Production, characterisation and supply of feedstock for BCO Task 2: Production and characterisation of BCO Task 3: Fast pyrolysis scale-up potential Task 4: Usage of BCO in a Stirling engine for electricity production, performance and emissions Task 5: Assessment of techno-economics including LCA and market studies EXPECTED ACHIEVEMENTS It is expected that immediately after the end of this project, pilot plants can be safely designed for different suitable sites in Europe for demonstration and market introduction. The consortium comprises well experienced partners from applied research and technological promotion, education, industry as well as an international org
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon Europe proposal for the project 'Defining the rhoptry kinome in Plasmodium falciparum and its importance for invasion of red blood cells'.

assistant: **Objectives**

Malaria remains a major global health issue, caused by the apicomplexan parasites of the genus Plasmodium, with Plasmodium falciparum being the most virulent and lethal species. Malaria symptoms primarily result from repetitive cycles of parasite invasion, replication and egress within human red blood cells (RBCs). Invasion by the infective merozoite forms is facilitated by the secretion of factors from apically localized secretory organelles. Among these, the largest, known as rhoptries, contain proteins essential during invasion and subsequent intracellular development. Despite their critical role, the regulatory mechanisms governing their functions remain poorly understood. For instance, phosphorylation has been observed in several of these essential rhoptry proteins. However, the implications of these modifications are unclear, as no kinase has yet been identified within the rhoptries. Therefore, I hypothesize that kinases localize to rhoptry organelles and play important roles in merozoite invasion and intraerythrocytic development. Preliminary data supports this hypothesis, by showing that FIKK5, a non-essential kinase previously thought to be exported into RBCs, localizes to the rhoptry bulbs. Based on this, I propose to identify essential rhoptry kinases in P. falciparum and investigate their regulatory impact on other rhoptry proteins, as well as in invasion and intraerythrocytic development, using state-of-the-art proteomic, genetic and cell biological approaches. To achieve this, I will: 1) define the rhoptry kinome in P. falciparum; 2) characterize the functions of rhoptry kinases during parasite development; and 3) assess the impact rhoptry kinase disruption on the protein environment within rhoptries. Ultimately, through this proposal I will generate the f
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'The fate and therapeutic potential of human neoantigen-specific T cells' demonstrating excellence, impact and implementation quality for a Horizon 2020 call.

assistant: **Abstract**

Cancer is a global health burden and the second leading cause of death worldwide. In recent years, the development of immunotherapy has revolutionised the approach to cancer therapy. A significant limitation, however, is that it is only effective in a subset of patients and the biggest challenges remain the identification of immunogenic targets on human cancers and overcoming immunosuppression. Neoantigens, which are newly formed antigens that arise from mutated tumour proteins, are promising targets in immunotherapy. With the proposed project, we aim to investigate the immune response against human neoantigens which will be induced in a novel humanised tumour mouse model. This unique mouse model harbours the entire human CD8 TCR repertoire and expresses human HLA-2. We will test whether human neoantigens can elicit spontaneous anti-tumour T cell responses in acute inflammatory versus resting conditions, ultimately leading to tumour rejection. We hypothesise that T cells are not able to reject tumours in absence of acute inflammation, similar to the development of most human cancers. To investigate why neoantigen-specific T cells become dysfunctional and fail to reject tumours, we will characterise the functional state and metabolic phenotype of neoantigen-specific T cells by flow cytometry, metabolomics and TCR sequencing. In addition, we will characterise the TCR repertoire of T cells that cause tumour rejection under acute inflammatory conditions. Lastly, we will compare the therapeutic efficacy of adoptively transferred T cells transduced with newly identified TCRs with neoantigen-based vaccines against large established tumours. Overall, this project will contribute to the understanding of immunosurveillance and establish novel techniques to test predicted neoantig
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon 2020 proposal on 'Understanding the initiation virulence gene expression in African trypanosomes'.

assistant: **Concept**

African trypanosomes are flagellated parasitic protozoans that cause the devastating, lethal disease sleeping sickness, as well as causing economically damaging livestock infections across sub-Saharan Africa. Trypanosomiasis was targeted for elimination by the WHO and others in the London declaration by 2020. Trypanosomes are transmitted by the bite of an infected tsetse fly, which restricts their geographic distribution. In the salivary gland of the fly, parasites coat their surface with approximately 10 million copies of a single variant surface glycoprotein (VSG) in a single layer which is protective against the mammalian immune system and prepares trypanosomes for infectivity. This VSG is expressed in a mono-allelic fashion from a specialized telomeric expression site. VSG coat is an important virulence mechanism; without this coat, parasites are rapidly lysed by mammalian complement. This project will address a fundamental question in trypanosome biology: How do African trypanosomes express a single VSG in its coat? I will address this questions primarily using single cell RNA-seq. First, I will establish an inDrops pipeline at the Institut Pasteur in collaboration with Prof. Andrew Griffiths at ESPCI (Paris) using cultured T. brucei, which will allow me to parameterize this novel single cell RNA-seq pipeline. Then using this protocol, I will generate single cell transcriptomes from tsetse fly derived parasites, constructing a gene expression profile for development of trypanosome infectivity. inDrops is a truly disruptive technology that allows massively parallel indexing of cellular mRNA, allowing transcriptomic analysis of thousands of single cells. These finding will have broad significance in the field; firstly, they will provide the first high-resolution sing
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Long-lived optical probes to image G-quadruplex DNA in live cells' (PLIM-G4), addressing dissemination and exploitation.

assistant: **Impact**

There has been increasing experimental evidence suggesting that tetra-stranded DNA structures (G-quadruplexes) play important biological roles in telomere function and maintenance, replication and transcription. The most direct evidence for their existence has come from immuno-staining in fixed cells as well as from recent deep sequencing studies. However, to date, we are still lacking tools that allows us to visualizing G-quadruplexes directly in live cells. While several small-molecule probes that fluoresce upon interaction with DNA have been reported, none of them have been successful at imaging G-quadruplexes in a cellular environment. This is mainly due to the fact that they rely on changes in intensity which are not possible to track properly in a cellular environment. Recently, the host lab reported a novel strategy to image G-quadruplexes in live cells. This approach makes use of the changes in emission lifetime (rather than intensity) of optical probes upon their interaction with different topologies of DNA. Since life-time is concentration independent, this approach can be successfully used to image G-quadruplexes in live cells. While this has proven to be a highly successful approach, it is still in its infancy since the probe developed so far has a number of limitations such as low brightness, relatively small lifetime range and low selectivity. Thus, this project aims to develop a new set of probes that address all these issues and use them to image the dynamics of G-quadruplexes in live cells in real time. I propose to develop platinum complexes (which 'switch-on' their phosphorescent upon interactions with DNA) with high affinity and selectivity for G-quadruplexes. To achieve this, novel approaches for automated synthesis and high-throughput analysis will
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Enhancement of clinical value of functional imaging through automated removal of partial volume effect' in the context of a Fifth Framework call.

assistant: **Objectives**

In medicine, images of the structure (e.g. MRI) and function (e.g. Nuclear Medicine techniques) of the brain are used. Functional images are hampered by low resolution, resulting in contamination of the signal of each region from the surrounding structures (Partial Volume Effect -PVE), limiting their applications, especially when atrophy is present (e.g. in Alzheimer's Disease). Functional images can be corrected knowing the shape and size of corresponding brain structures as assessed by structural imaging. This projects aims at: 1) setting upon automated method for correction of functional low-resolution brain imaging;2)validating it using specifically designed anthropomorphic phantoms;3) applying it on pre-existing normal volunteers and patients studies. This software, validated by a team of European Centres, will set a standard for FVE correction, providing a tool for excellence of European research and of health care in brain disease.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'The Medieval and Early Modern Nautical Chart: Birth, Evolution and Use' to be submitted under Horizon 2020.

assistant: **Abstract**

Of all the technical and scientific developments that made possible the early modern maritime expansion, the nautical chart is perhaps the least studied and understood. This fact is very surprising as it was through those charts that the newly discovered world was first shown to the amazed eyes of the European nations. Although the History of Cartography is a well-established academic discipline and old charts have been examined for many years, their detailed technical study is still in its infancy. What is the origin of the pre-Mercator nautical chart, how charts evolved technically over time and how they were used at sea are all critical questions that remain to be answered. I intend to approach these challenges in a truly interdisciplinary way, by using innovative and powerful tools as a complement to the traditional methods of historical research: analytical cartometric methods, numerical modelling and the examination of the manuscripts through special lighting. By applying these tools to a large sample of charts of various periods and origins, I aim to unveil hidden graphic content related to their construction and use, to characterize their main geometric features, to establish meaningful connections with contemporary navigational methods and exploration missions, and to numerically simulate their construction by taking into account the explanations given in the textual sources. The effectiveness of those techniques has already been demonstrated in my previous studies, such as in the solution of an historical enigma which had been alive for more than a century: the construction of the Mercator projection, in 1569. Now, I propose to handle a broader and more complex set of questions, which has eluded the historians of cartography for even a longer period. The clari