RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'T cell subsets underlying the rise, fall and recall of the Germinal Center' (2021-2027) targeting Horizon Europe.

assistant: **Abstract**

Follicular helper T cells (Tfh), through their cognate interactions with B cells, drive the Germinal Center (GC) reaction resulting in affinity matured memory B cells and Plasma cells, hallmarks of the adaptive immunity and underpinning our ability to counter pathogenic insults. For mounting a successful response against most pathogens, including the virus SarS-CoV-2 that has crippled most nations for over a year, Tfh cells are of central importance. Aberrant activation of Tfh has also been implicated in many diseases, including Multiple Sclerosis, Diabetes and Lymphoma. Understanding Tfh biology is essential for our understanding of the adaptive immune system and in our quest to develop better vaccines and therapeutic treatments, as it will enable us to modulate GC output. Through work over multiple years, the applicant has developed an understanding for Tfh as a highly dynamic population that require equally dynamic regulation. Understanding the true nature of Tfh, and of the T cell subsets that regulate Tfh, including the molecular mechanisms of regulation, will necessitate the combination of new tools with already existing state-of-the-art methods that is proposed herein. This will lead to a new understanding of how Tfh drive B cells throughout the GC, how they shape B cell responses, shedding light on unresolved issues such as how different quality of memory B cells is formed,  and how the unappreciated phenomenon of GC termination, proceeds.  Specifically this proposal will result in 1) A new definition of Tfh and of GC T cell regulatory subsets. 2) A mechanism for Foxp3+ T cell mediated regulation of GC B Cells and GC termination and 3) A new understanding of the origin and quality of Tfh memory and associated GC T cell memory.


**Keywords:** Tfh, Germinal Cente
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Strengthening regional stem cells based research for advancement of multi modal innovative strategy for modelling neurodevelopmental disorders' will create measurable scientific, societal and economic impact aligned with Horizon Europe.

assistant: **Impact**

Neurodevelopmental disorders (NDDs) caused by alterations in early brain development are a group of complex and heterogeneous disorders that give rise to the psychiatric conditions such as autism spectrum disorders, intellectual disability, schizophrenia and bipolar disorder. NDDs have a high socio-economic impact and patients with NDDs and mental health problems are geographically dispersed. Thus, establishment of high capacity regional centres with equivalent research capacity and codes of practice is needed in order to build global research activity. The goals of STREAMLINE are to enhanced strategic networking and reinforce research and innovation potential of the Institute of Molecular Genetics and Genetic Engineering (IMGGE) in order to develop IMGGE as a high capacity hub for research of NDDs in the Western Balkans which is able to integrate with similar centres in Europe. This will be achieved by twinning IMGGE with three top-class research institutions in Europe: Cardiff University, University of Maastricht and Centre for Research and Technology. The project objectives are: a) to enhance the current and scale up the overall strategic networking activities between the IMGGE and internationally-leading European partners and regional partners; b) to raise the research profile of research stuff and scientific attractiveness of IMGGE in the field of NDDs; c) to strengthens research management capacities and administrative skills of the IMGGE staff and d) to become a regional leader in innovations tackling NDDs. Objectives will be achieved through networking, staff exchanges, training, summer schools, symposia, workshops, participation at conferences and dissemination in scientific community, industries and public. Twinning with partners will provide access to new res
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Treatment of long term irradiation embrittlement effects in RPV safety assessment' under Seventh Framework.

assistant: **Concept**

In view of the increasing age of the European NPPs and envisaged life time extensions up to an EOL of 80 years, there is a need for an improved understanding and prediction of RPV irradiation embrittlement effects connected with long term operation (LTO). Effects caused by high neutron fluences such as the possible formation of Late Blooming Phases and as yet unknown defects must be considered adequately in safety assessments. However, the surveillance database for long irradiation times (>20 years) and low neutron flux is sparse, which leads to uncertainties in the treatment of LTO irradiation effects. In this context microstructural data are essential for the understanding of the involved mechanisms. The proposed project aims at: 1) improved knowledge on LTO phenomena relevant for European reactors; 2) assessment and proposed improvements of prediction tools, codes and standards; 3) elaboration of best practice guidelines for irradiation embrittlement surveillance. The suggested scope of work is: i) Summary of boundary conditions for LTO and systematic (re)evaluation of the international prediction procedures of irradiation embrittlement ii) Generation of microstructural data of irradiated representative or original RPV materials; demonstration that damage models are (or not) consistent with the mechanisms of irradiation damage in the LTO (use of PERFORM60 prediction tools) iii) Investigation of specific LTO relevant phenomena like late blooming phases and flux effect from available results; role of Cu, Ni, Mn, P, Si under the aspect of LTO; iv) Correlation of microstructural data with mechanical properties and identification of the most important influencing factors (link with PERFORM60) v) Influence of high neutron fluences on fracture toughness curves shape vi) Com
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Information system development & embedded engineering documentation' (INDEED).

assistant: **Innovation**

New software development techniques and fast changing business environments, bring frequent design changes during system development projects. To cope with this situation, all involved parties must be informed adequately, within minimum time frames. Objective is to integrate documentation production, incl. distribution & release management in the development process.THE EXPERIMENTLotus-based toolkits will be introduced and implemented in the baseline project for Amsterdam Airport Schiphol. This is the second of a kind, to be developed in co-operation with Scheidt & Bachmann (Germany). The results (quality of the delivered system) will be analysed against those of the preceding project.EXPECTED IMPACT AND EXPERIENCEThe software system, to be delivered from the baseline project should show better quality, and require less on site testing and reach a stable status sooner resulting in the following experiment objectives:A 50% reduction in failures after deliveryA 20% reduction of development time until moment of stable systemsA 50% reduction of acceptance tests,All compared to the preceding project with Amsterdam Airport.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Group Stability and Quantum Information Theory' (GRPSTABQIT) under Horizon Europe.

assistant: **Objectives**

The main focus of the project will be on stability properties for groups and their connections with quantum information theory. Intuitively, the term stability refers to the phenomenon of when approximate properties are ""close"" to actual properties. Concretely, the project will introduce for the first time stability with respect to quantum groups, and provide a systematic approach towards different important notions of stability for various classes of groups, notably ""graph product-like"" groups and amenable groups. For the latter, stability is also connected to character theory and dynamical systems, which will allow for different techniques from these areas to be applied towards our results.""


**Keywords:** Hilbert-Schmidt stability, permutation stability, quantum groups, (graph) products of groups, character theory of amenable groups, quantum information theory
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Structure-function studies of the human FASTK family of mitochondrial proteins with putative novel RNA binding domains – the helical FAST motifs and the small RAP domain' to be submitted under Horizon 2020.

assistant: **Abstract**

The purpose of this project is three-fold. The main aim is to elucidate the structure of two novel RNA binding folds: the helical FAST motifs and the small RAP domain that are predicted in the Fas-activated Serine/Threonine Kinase (FASTK) protein family. To this purpose, I will employ protein crystallography and small angle X-ray scattering to obtain models of FASTK proteins and their domains. The second objective is to understand the function of the FASTK family of mitochondrial RNA-binding proteins that were originally annotated as atypical kinases. The eponymous FASTK engages in processes similar to its interaction partner, the post-transcriptional regulator TIA-1, whereas some of the other five human paralogs are necessary for cellular respiration. I will use purified FASTK proteins to study their enzymatic activities and RNA-binding properties in vitro, which should clarify their functional classification as kinases or post-transcriptional regulators. Additionally, follow-up studies may include validation of the findings through informed mutagenesis, identification of RNA targets, protein partners, and selected phenotypes in RNA biology. The final goal of this project is to enable a young researcher to establish herself as an independent researcher, creating a group which will focus on mitochondrial RNA-binding proteins and employ X-ray crystallography as the main tool. Altogether, this interdisciplinary project will 1) Impact various fields of research, such as structural and RNA biology 2) Has the potential for applications in human health due to the connections of the FASTK family to mitochondrial and inflammatory diseases, 3) Significantly develop the career of a talented researcher and 4) Contribute to the academic and research excellence at the University of
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'RESOURCE EFFICIENT AND SAFE FOOD PRODUCTION AND PROCESSING' under Seventh Framework.

assistant: **Concept**

RESFOOD adresses on the most important topics in the food chain food towards resource efficient and safe food production and processing, leading to maximised resource productivity and recycling and re-use of valuable materials by research and demonstration of the proposed green solutions: Increased output with reduced input.Many  natural resources (e.g. minerals, water, soil, biomass, land and fuels (energy) are used to grow and process food products, but in many cases their usage is highly inefficient, due to the lack of technological solutions and knowledge in combination with uncertainties about health and safety issues. Another important challenge in the food chain management is the large amount of wasted food. RESFOOD will overcome the main bottlenecks and barriers leading to an Resource Efficient Food Chain by:• Developing innovative technologies for re-use of Nutrients, Energy, Water and Biomass, reducing input, maximizing resource productivity and minimizing waste• Develop new methods for improving the disinfection processes for vegetables ensuring appropriate monitoring of health and safety risks.• Validate the solutions in five on site pilot demonstrations, also including Life Cycle AssessmentIn the RESFOOD resource efficiency concept the focus is a cascade approach: Look first for the most efficient solutions with the lowest effort, like direct re-use of warm and cold water (and energy) nutrients and biomass, followed by more complex solutions like withdrawal of useful products and energy  from the water and the recovery of high valuable components from food waste (biomass).This will lead to 30 to 75 % reduction of water, energy and nutrients use, 25 to 80 % less emissions to surface-  and ground water, 20-30 % reduction of the use of fertilizer products and
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon 2020 proposal on 'Malaria Parasite Networking:  Discovering Modes of Cell-Cell Communication'.

assistant: **Innovation**

Malaria, caused by Plasmodium falciparum, is a devastating parasitic disease effecting hundreds of millions of people worldwide. The parasite’s transmission cycle between humans and mosquitoes involves a remarkable series of morphological transformations. While it is clear that, for such a complex journey, the parasites must develop means to sense their host and coordinate their actions; these modes of communication remain one of the greatest mysteries in malaria biology. In fact, since an individual parasite is enclosed by three membranes inside its human host, the red blood cell (RBC), they were not thought to possess any communication ability. However, we discovered that these parasites, despite the multiple barriers, are able to communicate and exchange episomal genes by releasing exosome-like vesicles, thereby opening the exciting new field of malaria parasite communication. Our initial data demonstrate that these vesicles serve as a secure tool for the delivery of remarkable components. The overarching goal of this proposal is to take an innovative look at this under-investigated area of parasite sensing and signalling pathways and to decipher the multiple layers of parasite and host signalling networks. Specifically, we will determine the biological roles of Plasmodium exosome cargo components in: parasite-parasite communication - exploring parasite coordination traits in cell-density growth and sexual development (Objective 1); and parasite-host communication - unravelling the mutual communication of the parasite and its hosts, the red blood and immune cells (Objective 2). Simultaneously, we will exploit our experience in cell communication research to investigate the complementary, yet-to-be-explored mode of parasite communication via the secretion of small mol
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'New pathway and small-molecule compounds for Parkinson’s disease therapy' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

Parkinson’s disease (PD) is a neurodegenerative disease with growing incidence. More than 10 million people worldwide are affected by this condition. In addition to decline in the patients’ quality of life, the economic burden of PD is estimated to exceed 13,9 billion EUR in EU annually. Considering this, novel and more efficient therapeutic options are needed, as current PD treatments alleviate symptoms without stopping neurodegeneration and progression of the disease. Although the etiology of PD is unclear, degeneration of dopamine (DA) neurons has been shown to result from certain genetic mutations, toxins and pathological protein aggregation among others. Importantly, recent evidence points to decrease in mRNA methylation at adenosine N6 (m6A) in PD. Data also indicate that m6A reduction may induce DA neuron apoptosis.Prof. Mart Saarma and collaborators have discovered that m6A methylation is a crucial regulator of neuronal apoptosis. They developed novel m6A methyltransferase activators and demethylase inhibitors that potently enhance mRNA m6A levels. Importantly, preliminary data show that these compounds protect DA neurons from neurotoxin-induced death in culture at nanomolar concentration. Moreover, one compound tested thus far remarkably improves motor behavior, protects and regenerates DA neurons and their axons in rat PD model more potently than “gold standard” GDNF. Considering this, the current project aims to elucidate the mechanisms how m6A regulates DA neuron survival and axonal regeneration. We will identify for the first time the mRNAs with increased/decreased methylation at adenosine N6 that regulate the life and death of DA neurons, thereby potentially opening new directions in PD research. In addition, we aim to provide completely new lead molecules
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon Europe proposal on 'A novel immuno-oncolytic virus-based dendritic cell therapeutic cancer vaccine'.

assistant: **Concept**

Following decades of development, therapeutic cancer vaccines are beginning to gain momentum in demonstrating therapeutic effects, and they represent a particularly promising approach. The aim of therapeutic cancer vaccines is to stimulate immunity against tumor antigens, usually through the use of whole cells, peptides, or nucleic acids (i.e. mRNAs). Dendritic cell- (DC) based vaccines represent particularly interesting candidates for therapeutic cancer vaccine products due to their ability to cross-present antigens to T cells, thereby driving adaptive immune responses through induction of antigen-specific cytotoxic T lymphocytes. Despite the potential of DC-based cancer vaccines, several major limitations have hindered their success in clinical studies. These limitations include insufficient ex vivo maturation of DCs and challenges in selecting optimal tumor antigens to target.   The aim of DCanVAX is to develop a novel oncolytic virus-based dendritic cell vaccine approach for systemic therapy of solid cancers. We propose that the use of a highly immuno-oncolytic virus as a mechanism to lyse tumor cells ex-vivo, will lead to the release of the entire tumor antigen library, as well as a cocktail of danger signals in response to the virus infection. By applying this highly immunogenic lysate to autologous DCs, an optimized and personalized DC vaccine can be generated. This method overcomes the major challenges that have been encountered by DC vaccine approaches in the clinic, by providing a potent mechanism of DC maturation and activation, while simultaneously exposing the DCs to the complete repertoire of tumor antigens that are specific to the patient, leading to potent and broad tumor-specific immune responses against the cancer. This approach can be developed for mu
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'Structure-function analysis of mRNA metabolism by Ccr4-Not'.

assistant: **Objectives**

Eukaryotic gene expression is tightly regulated at all stages of the mRNA life cycle, from mRNA synthesis in the nucleus to mRNA degradation in the cytoplasm. Defects in gene regulation cause serious defects in cells, and severe pathologies in humans, such as cancer, diabetes, and cardiovascular disease. The host lab recently made the seminal discovery that mRNA synthesis and degradation are coupled processes. The nine-subunit Ccr4-Not complex emerged as a candidate for a so far elusive coupling factor: in contrast to most mRNA processing proteins that act at only a single step in the mRNA life cycle, Ccr4-Not acts throughout the mRNA life span in mRNA synthesis, transport, translation and degradation.  Despite its importance, the function of Ccr4-Not in mRNA synthesis is poorly understood. Although a recent publication suggested that Ccr4-Not could rescue stalled RNA polymerase and thereby advance transcription, the rescue mechanism remains enigmatic.Our key research objectives are a comprehensive structure-function analysis of Ccr4-Not in complex with transcribing RNA polymerase II, determination of the genome-wide mRNA-protein contacts that Ccr4-Not undergoes, and ultimately a description of the mechanisms by which the by Ccr4-Not promotes transcription and regulates the mRNA lifecycle.The host’s  broad multi-disciplinary expertise and the established state-of-the-art techniques uniquely position us to achieve our ambitious objectives. All required protein complexes can be purified in milligram amounts. We will employ an innovative combination of cutting-edge methods such as high-resolution cryo-electron microscopy, molecular interactions assays, isotope-tagged cross-linking, proteomics, genome-wide RNA interaction assays in vivo, and bioinformatics.We expect our res