RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Chemotherapy guidance based on efficacy of treatment regimen in cancer cells from the individual patient' (CHEMOGUIDE) targeting Seventh Framework.

assistant: **Abstract**

The ChemoGuide project will clinically validate a proto-type technology (2treat) developed in the previous “Research for SMEs” project, “Integrated Chemical Synthesis and Cell screening in patient cells (ICSC). The project will execute a clinical study to evaluate the 2treat test’s capability of predicting which combination therapy that is most efficacious and show no resistance in the individual colorectal cancer patient. The prediction is based on reading the cell functional output cancer cells isolated from the individual patient. The clinical study will serve to verify the capability and robustness of the 2treat test. The 2treat test is formatted as an easy-to-use, disposable test array.Colorectal cancer is now the most diagnosed cancer in EU, and the prognosis of especially stage III and IV patients is very poor. The 2treat test is expected to provide a major benefit to these patients by significantly improving the Prognosis Free Survival (PFS). Importantly oncologist will be provided the first technology that is capable of testing the cell-functional output of all combination treatment regimens used for treatment of colorectal cancer in Europe and USA before a first line treatment is launched. The technology will both improve treatment quality for the individual patient and raise the cost efficiency providing a positive impact on the health care systems.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Quantification of the role of mechanical stresses in plant cell morphogenesis.' to be submitted under Horizon 2020.

assistant: **Abstract**

Specific cell and tissue form is essential to support many biological functions of living organisms. During development, the creation of different shapes fundamentally requires the integration of genetic, biochemical and physical mechanisms.In plant, a stiff pecto-cellulosic network encapsulates cells and counterbalances stress created by turgor pressure inside the cell, thereby controlling cell shape. It is well established that the cytoskeletal microtubules network play a key role in the morphogenesis of the plant cell wall by guiding the organisation of new cell wall material. Moreover, it has been suggested that mechanical stresses orient the microtubules along their principal direction, thereby controlling wall architecture and plant cell shape. Nevertheless, to fully understand how plant cells are shaped and how mechanical stresses influence this process, a quantitative approach needs to be established.In this project, we aim to provide new fundamental knowledge on the role of mechanics in plant development at the cellular scale. New experimental and imaging methods are now available to achieve this aim. We will combine experimental approaches and mechanical modeling to study quantitatively how single plant cells respond to mechanical signals and how they are integrated by the cell into changes in genetic expression. The outgoing host at Caltech, and the candidate have had success developing a custom-made micro-wells device to mechanically disrupt single plant cells. By coupling this approach with mechanical modeling and using a novel software developed by the returning host at the Sainsbury Laboratory, this project will lead to fully develop a computational model of plant cells and tissues morphogenesis, as they respond biologically to changes in directions and a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Chaos and thermal effects in black hole interactions' in the context of a Horizon Europe call.

assistant: **Objectives**

The understanding of the microscopic origin of chaos and thermal effects in the physics of black holes is very challenging. Such fundamental aspects seem to be intimately connected, but a microscopic analytical description of their interplay is still missing. The quantum gravity description of these aspects could help clarify their microscopic interconnection. The project I propose has the ambition to probe black hole states through highly excited string and coherent string states, providing a quantum microscopic description of chaos, thermal effects and information theory. The main observables are scattering and decay processes. The research programme contains the following parts. First, a general strategy will be developed to measure, and analytically control, chaos in physical processes involving black holes, such as black hole decays and black hole Compton scattering. In particular, a quantitative measure of chaos will be introduced using Random Matrix Theory techniques and the relative Lyapunov exponents will be computed. Second, in order to study thermal effects in black hole processes, the triggering of the thermalization phase driven by chaos will be investigated. Finally, the project will consider the following direct applications. The first one will be the study of chaotic signatures in wave forms and quasi-normal modes of gravitational and electro-magnetic waves; the second will concern the realization of black hole horizons in terms of highly excited string and coherent string form factors.


**Keywords:** Black holes, thermalization, chaos, string theory, string excited states, string scattering
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Understanding changing approaches to organising and managing marketing activity in the larger sme' will create measurable scientific, societal and economic impact aligned with Fourth Framework.

assistant: **Impact**

Research objectives and content In the management of the larger SME - which lacks the resources of the large firm and the organisational flexibility of the small firm - marketing activity has been experiencing a transition, if not a diminution, in its role. Many shortcomings have been identified in marketing's ability to organise itself and 'deliver' full customer satisfaction. Other business functions such as manufacturing and logistics appear in the ascendant. The objective of this research is to examine and comprehend changes in the approaches and structures to organising and managing marketing activity in the larger SME. It will study current practice in a range of firms using an in-depth case study approach. The proposer is currently researching a number of such cases in the Republic of Ireland. The opportunity to work in the University of Ulster would afford the researcher the possibility to link into similarly focused research being carried from this campus, and to replicate such cases in sites in the UK and other EU locations. In studying how marketing is managed and executed, the research agenda will move beyond the conventional triangulated paradigm of structure-systems-shared values to draw on concepts of business reengineering, order fulfilment, service blueprinting and the use of teams and team building. Training content (objective, benefit and expected impact) The benefit of this research would be, hopefully, a deeper understanding of marketing best practice in larger SMEs and an ability to make recommendation to management about optimal marketing investment and the development of marketing competence in such firms - in effect, to rejuvenate the role of marketing. Links with industry / industrial relevance (22) Given the nature of the research and methodol
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Improving therapy and intervention through imaging' (TAHITI).

assistant: **Innovation**

The scientific objective of the programme is to design, develop, and validate new imaging technologies to improve the performance and safety of therapeutic and interventional procedures.  Improving therapy and surgery planning will be approached using multimodal imaging strategies, lesion and dysfunctional tissue characterization, efficient segmentation and image fusion techniques. Image guided therapy and intervention improvements will be pursued by designing the integration of new imaging modalities in the surgical workflow, providing new techniques to compensate for undesired motions as well as innovative methods and instruments to integrate new intraoperative imaging devices. Follow-up and therapy outcome is a major concern and will be approached using innovative methodologies to quantitatively assess and predict recovery and prognosis after therapy or surgery. Furthermore, concrete scenarios will be considered within the frameworks of interventional cardiac therapy and surgery and soft tissue organs therapy. New workflows will be proposed incorporating the new technologies in these concrete scenarios.The objectives to add value to the ERA will be:•To develop new techniques and methodologies to assist in the development of new therapy surgical procedures. This will clearly improve the position of the European partners towards technology transfer to the healthcare technology industry.•To improve the healthcare of European citizens by providing new and safer means of therapy and surgery.•To increase the skills and knowledge of the EU partners in order to better perform clinical translational research implying new technologies.This project is a multidisciplinary initiative allowing the exchange of scientists among four research institutes with international recognition
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'European Research Training in the Ubiquitin Proteasome System' under Seventh Framework.

assistant: **Concept**

The main objective of the international training network UPStream is the training of a novel generation of European scientist in a critical and complex field of modern biology: the understanding of the regulation of the UPS and its potential use for drug development. The importance of this process is illustrated by the imperious necessity to destroy certain proteins during several phases of the cell cycle in order to progress to the next phase. The biochemical process of ubiquitylation is an efficient way to label proteins that will be targeted to degradation by the proteasome at the right time and cellular compartment, according to the cell necessities. In addition, this post-translational modification also offers to the cell the possibility to control other processes related or not to proteolytic functions. Indeed, the attachment of ubiquitin (Ub) or ubiquitin-like (UBL) molecules such as SUMO and NEDD8 does not always result in proteolysis, but can induce conformational and/or interaction changes driving a large diversity of effects. Considering the interconnectivity among Ub and UBL molecules, we end up with an extremely complex system regulating protein activity and stability. Given the implications of the ubiquitin proteasome system (UPS) in many essential cellular processes, the technology required to study this system is very diverse. As part of the proposed network the latest technological developments (please see implementation) will be used to explore chemical, biochemical, molecular, developmental and genetic aspects of the UPS. This research area has become an area of investigation by itself, very attractive for fundamental scientists as well as for the pharmaceutical industry aiming to identify potential targets for drug development to be used in many dise
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon 2020 proposal on 'Control of Bacterial Multidrug Tolerance and Stress Response by Alarmone Synthetase SpoT'.

assistant: **Innovation**

Difficult-to-treat chronic and recurrent bacterial infections are often caused by bacteria that are sensitive to commonly used antibiotics. The reasons for this recalcitrance are frequently unknown. However, when grown in the laboratory, all bacteria, including major pathogens form persister cells that are multidrug tolerant, a phenomenon thought to be a major factor underlying recalcitrant infections. We observed that the general bacterial stress response, known as “the stringent response”, plays a key role in persister cell maintenance. Indeed, stochastic variation of the stringent response regulator ppGpp triggers persister cell formation. However, the molecular mechanisms by which environmental cues activate the stringent response are still largely unknown and represent one of the most fundamental, unsolved problems in prokaryotic molecular biology. Importantly the stringent response is also required for virulence of almost all bacterial pathogens, strongly arguing that novel insights into ppGpp biology will lead to novel methods to combat infections. We recently observed that the ppGpp synthetase II activity encoded by SpoT is responsible for persister cell formation in Escherichia. coli. Therefore, I propose a research program that builds on the pivotal role of SpoT in bacterial persistence, with the goal of dissecting the molecular mechanisms by which environmental stimuli trigger SpoT-dependent ppGpp synthesis. This project has three main objectives: (i) To unravel how spoT expression is regulated (ii) To reveal how ppGpp synthetase II activity is mechanistically controlled and (iii) To decipher the physiological role of SpoT in persister cell decision-making. The program is ambitious and will provide a significant step forward for the persistence field and offe
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Comprehensive data-driven Risk and Threat Assessment Methods for the Early and Reliable Identification, Validation and Analysis of migration-related risks' under Horizon 2020.

assistant: **Concept**

EU borders are constantly faced with a multiplicity of challenges, from increased waves of illegal migration to human trafficking, document fraud, terrorism, smuggling, and public health threats. In the CRiTERIA project we will develop a novel risk analysis methodology, which is, on the one hand, clearly rooted and builds upon existing methodology such as CIRAM and, on the other hand introduces novel more complex and effective indicators, which overcome important limitations of existing models. Such extended risk and vulnerability analysis methodology has to be backed by effective intelligent analysis technology. Building upon existing text, media, data and network analysis technology, in CRiTERIA, we will develop and evaluate advanced analysis technologies and tools that are tailored to the new comprehensive threat indicators of the CRiTERIA methodology. Special focus will be put to the consideration of the role of narratives, events, attitudes, and to the vulnerability of borders and humans as well as on providing semi-automatic tools and methods for risk-related evidence validation and explanation, for identifying risk propagation and interlinking, thus supporting decision processes in risk analysis in an innovative way. When developing this holistic CRiTERIA risk and vulnerability analysis framework for border agencies ethical, legal and societal aspects will be considered from the very beginning. The methodology will be developed in close collaboration with practitioners from border agencies, which will also validate the developed methods and technologies in piloting activities.For achieving its goals CRiTERIA brings together an interdisciplinary team of experts including researchers in the fields of security and risk analysis, in the field of data and media analys
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Silencing RNAs: organisers and coordinators of complexity in eukaryotic organisms' will create measurable scientific, societal and economic impact aligned with Sixth Framework.

assistant: **Impact**

A recently discovered layer of gene regulation in eukaryotic organisms employs small regulatory RNAs (miRNAs and siRNAs). These RNAs are 20-28 nucleotides long and are produced by Dicer ribonucleases acting on double-stranded RNA precursors. Together with an effector protein complex, they scan for complementary RNA or DNA so that expression of these targeted molecules is silenced at the transcriptional or posttranscriptional levels. These short RNAs influence gene expression during growth and development and initiate epigenetic changes to DNA and chromatin. SIROCCO will characterize the full complement of miRNAs and siRNAs in animals and plants. Using bioinformatics, genomics, biochemistry, cell biology and genetics, the sortium members will reveal how these RNAs are produced and processed, how they are transported and how they target specific genes and RNAs for silencing. SIROCCO will investigate the miRNA and siRNA profiles associated with development, with phenotypic divergence within populations, and with diseased states including cancer. The functional genomics of silencing RNAs will be addressed by up- or down-regulation of miRNA and siRNA species. There will also be an assessment of miRNA and siRNA regulatory networks and their interaction with other cellular trol mechanisms.The outputs of SIROCCO will include databases of silencing RNA sequence and function in several organisms, new technologies for detection and manipulation of these RNAs, and information that will allow siRNA and miRNA profiles to be used as molecular markers and diagnostic methods for natural biological variation including the perturbations associated with disease. SIROCCO will also identify potential targets of disease therapy amongst the components of the small RNA silencing systems.Finally
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Investigating the LRRK2-melanin connection through phosphoproteomics of isogenic melanoma cells' (MELANOPARK) targeting Horizon 2020.

assistant: **Abstract**

LRRK2 is a multi-domain protein, which uniquely possesses both protein kinase and GTPase catalytic activities. Autosomal dominant mutations of LRRK2 represent one of the principal genetic risk factors for Parkinson’s Disease (PD), which results in preferential loss of neuromelanin containing, dopaminergic neurons from the substantia nigra. The (patho)physiological roles of LRRK2 are currently unclear. However, it has been linked to the regulation of endolysosmal membrane trafficking through complex formation with other PD-related gene-products (Rab7L and Vps35). LRRK2 is also highly expressed in skin pigment cells (melanocytes), which specifically produce melanin within the lysosome-related organelles known as melanosomes, in a process referred to as melanogenesis. Here we propose to examine the substrates and function of LRRK2 in pigmented melanoma cells. We will generate a MNT1 cell panel expressing PD-associated mutations of LRRK2 using CRISPR/Cas9 gene editing and characterise the respective (phospho)proteomes ±LRRK2 inhibitors from total lysates and from melanosome-enriched fractions using quantitative mass spectrometry. Melanosome biogenesis itself will be compared across the panel using electron microscopic procedures established by the experienced researcher as well as other organelle associated parameters (e.g. contacts with mitochondria) and trafficking pathways linked to Vps35 and Rab7L. The aim is to establish LRRK2 substrates or cellular consequences of activity, which may be specific to melanin-containing cells and link these to PD or to the increased incidence of melanoma observed in PD patients.