RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'ANALYSIS OF PML/RARA ONCOGENIC COMPLEX IN ACUTE PROMYELOCYTIC LEUKEMIA' (APL).

assistant: **Concept**

Acute promyelocytic leukemia (APL) appears to be primarily a monogenic disease characterised by a specific t(15 ;17) translocation that fuses the PML and the RARA gene to generate a PML/RAR fusion protein. PML/RARA is a transcriptional repressor, and RARA homo-dimerisation through the coiled coil of PML was believed to be the basis for repression via enhanced binding of co-repressors.  Clinically, APL is exquisitely sensitive to retinoic acid (RA), arsenic trioxide and cAMP. This cardinal feature of the disease has been used to model its pathogenesis by analyzing the response to therapy of primary cells in vivo and ex vivo.Our aim is a further understanding the pathogenesis of acute promyelocytic leukemia, in particular the molecular bases of its response to RA, arsenic and cAMP. This will be essentially achieved through the analysis of the phenotype and transcriptome of cells or animals expressing PML/RARA mutants, by applying innovative approaches in cellular and molecular biology, such as purification of tagged proteins and ChIP.We propose two main objectives:- Partners identification of the oncogenic PML/RARA complex. Subsequently, we will analyse the functional domains of the complex and the changes in the complex induced by RA, arsenic and cAMP.- Analysis of PML/RARA-dependent target gene repression.  We will compare between different PML/RARA mutants in respect to the chromatin or DNA status of the target gene promoters.This project addresses new therapeutic targets in APL and other types of cancer where RARA is implicated. It is a multidisciplinary project that involves a highly valuable education for the candidate in new scientific aspects related to her research field.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Multi-agent architecture for distributed-in load control and overload protection' (MARINER) under Fourth Framework.

assistant: **Objectives**

Main ObjectiveThe main objective of the MARINER project is to assess the usefulness of agent technology for the enhancement of the performance and management of complex distributed telecommunications network environments. Agent technology offers the opportunity both to deliver intelligence to the end-user and to organise intelligence in the network itself, thus its successful application will greatly aid the future evolution of the telecommunications infrastructure. Recent years have seen the development of a variety of agent languages, architectures and platforms. However, efforts towards the standardisation of agent facilities have only started recently, mainly within the Foundation for Intelligent Physical Agents (FIPA) and the Object Management Group (OMG). MARINER will seek to validate the FIPA and OMG models for agent based systems through the design, implementation and trialing of a prototype multi-agent system for Distributed Intelligent Network (IN) load control, which will need to satisfy stringent real-time performance constraints.It is widely agreed that Intelligent Network distribution (facilitated through the introduction of CORBA into IN network elements) will shortly be required to meet the increasing demand for the rapid and cost effective introduction of telecommunications services such as multi-operator Freephone and Virtual Private Network (VPN). In this context, it will be critical to ensure that the performance of such services is not degraded due to network congestion problems. Given that reactivity and pro-activity are characteristics of agents and multi-agents systems, it would appear that the adoption of an agent-based approach may offer a timely solution to the problem of efficient load control in Distributed-IN network environments.Technical
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Fourth Framework proposal on 'The role of plasma membrane structure in prion propagation, transport and pathogenesis'.

assistant: **Concept**

Kuru, Creutzfeldt-Jakob disease, and Gerstmann-Sträussler syndrome illustrate the infectious, sporadic, and genetic manifestations of human prion diseases while scrapie of sheep and bovine spongifonn encephalopathy are the most common forms in animals. These diseases are a major health concern in European countries because of the potential link between bovine spongiform enncephalopathy and an increasing number of human spongiform encephalopathy that have recently appeared in Europe.  The only factor known to be necessary for transmission is the scrapie prion protein (PrPSsc) which is an abnormal isoform of an endogenous cellular protein (PrPC) found mainly in neurons. Current evidence argues that direct interaction of PrPSc with prpc is necessary for the transformation prpc + PrPSC -> 2 PrPSc. Both PrPSc and PrPC are localized on plasma membranes in cholesterol-rich segments called rafts.Our first objective is to improve isolation of plasma membranes by chemical vesticulation of scrapie-infected neuronal cells and to improve the isolation of rafts by various density gradient ultracentrifugation steps These structures will be characterized both in lipid and protein composition and their lipid composition will be modified by using different metabolic inhibitors in order to define the environmental prerequisites for PrPsC formation. We have recently found that treatment of brain homogenates from overexpressed PrPC mice with various prooxidants converts the protein to protease-resistant product similar to PrPSC. Our second objective is to study this transformation process and to define, whether prpc is modified through direct free radical attack or by secondary lipid peroxidation products. We also plan to investigate the role of oxidative stress in prion-infected GT1-trk ce
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'WELFARE AND HEALTH IN SUSTAINABLE AQUACULTURE' (WEALTH), addressing dissemination and exploitation.

assistant: **Impact**

Despite the development of more efficient diagnosis, sanitary trols and preventive methods such as vaccination, disease incidence and use of antibiotics and drugs still remain as problems for the European aquaculture industry. The objective of the present project is to improve welfare and health of farmed fish through reduced stress and improved immune competence in Atlantic salmon and sea bass. The principles derived are expected to be applied to other species. The objective will be achieved by exposing fish in fresh water and seawater to environmental stress such as low O2, ammonia, high CO2 under high and low stocking density, and varied light regimes. In selected studies, implantation of stress hormones is carried out to mimic stress ditions. Stress responses will be assessed by analysis of plasma hormone levels as well as by a set of biochemical and physiological stress indicators in plasma and tissue of experimental fish. The experiments will also explore the underlying physiological and molecular mechanisms of stress effects on primary barriers to infection and on behaviour. In addition, immune competence offish exposed to Stressors will be assessed by innate and acquired immune parameters and the bi-directional communication between the endocrine and immune system will be investigated. Due to limited number of tools available in fish immunology, the project also aims at developing new molecular methods to understand the underlying mechanisms of the stress induced immune compromised fish. In addition, susceptibility to infections will be studied by challenge experiments with selected bacteria and viruses. Based on the results obtained in the experimental part of the project, operational husbandry protocols for improved welfare and health of farmed fish including
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon Europe proposal on 'Design of Flow-Photoelectrolyzer'.

assistant: **Innovation**

The EU strongly strives for a sustainability transformation, and the EU Commission's Green Deal accelerates climate neutrality by 2050, with a modern and competitive economy. Climate neutrality of the chemical industries calls for innovative resource- and energy-economical technologies to harvest renewable forms of energy and prevent chemical waste. Breakthrough studies demonstrated the unique potential of harnessing both solar and electrical energy for sustainable syntheses. Thereby, chemical waste formation can be minimized, while enabling innovative scenarios that neither electrochemistry nor photochemistry can achieve. Specifically, the merger of photoelectrochemically-enabled molecular assembly with the cathodic hydrogen formation constitutes ideal resource-economic synthesis. Unfortunately, photoelectrocatalysis continues to be limited to reactions on small mmol lab-scale, preventing the translation of recent exciting trends to the next level of industrial process scale. The absence of a commercially available reactor represents a major limitation to unleash the full potential of photoelectrochemical chemistry for practitioners. The transfer to continuous flow bears great opportunities for photoelectrochemistry in chemical, agrochemical and pharmaceutical industries. The overarching objective of PhotoElectroFlow is to devise a first continuous flow electrolyzer for scalable photoelectrochemical transformations in a safe and robust manner on gram to kilogram scale. Thus, we will provide a most-user-friendly technology, closely collaborating with partners in industry, to harvest the unique synergy of light and electricity. Evaluation of the PhotoElectroFlow electrolyzer will be benchmarked in molecular transformations of practical importance to chemists in academia
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Elucidating the Molecular Mechanism of  Myoblast Fusion in Vertebrates' in the context of a Horizon 2020 call.

assistant: **Objectives**

Cell-to-cell fusion is a ubiquitous phenomenon essential for the physiological function of numerous tissues. A striking example is the fusion of myoblasts to form multinucleated myofibers during skeletal muscle development and regeneration. During myoblast fusion, membrane architecture must be radically remodeled. Yet, how membrane remodeling occurs on the molecular level is poorly understood as, until now, there was no approach available for visualizing dynamic changes in the cellular ultrastructure and the organization of the fusion machinery in situ.To fill this gap, we have developed correlative light and 3D electron microscopy (CLEM) methods that allow us to identify fluorescent signals within EM samples with high sensitivity and subsequently localize the source of these signals with high precision. In this proposal, we will apply these methods in combination with live-cell imaging, biochemistry and cryo-electron tomography (ET) to deliver fundamental knowledge about the mechanism of myoblast fusion. Our specific aims are:Aim 1: To resolve the molecular and ultrastructural events underlying cell fusion, by revealing how plasma membrane architecture is remodeled at sites of fusion using 3D EM.Aim 2: To dissect the mechanism driving membrane remodeling during fusion, by visualizing how the fusion machinery assembles at sites of fusion and how its assembly is mirrored by changes in membrane shape, using biochemistry and live-cell imaging.Aim 3: To determine the structure of the fusion machinery in situ, by using cryo-ET and subtomogram averaging.Our synergetic experimental strategy will generate a quantitative, dynamic high-resolution view of the fusogenic synapse of vertebrate muscle, revealing how the fusion machinery remodels the plasma membrane at sites of fusion.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Bioinformatics for spatial metabolomics' (METASPACE).

assistant: **Innovation**

Metabolomics is recognized as a crucial scientific domain, promising to advance our understanding of biology, physiology, and medicine. The emergence of high-resolution imaging mass spectrometry (HR imaging MS) opened doors to spatial profiling of hundreds of metabolites directly from tissue sections. However, clinical use of HR imaging MS is hampered by a lack of clinically-oriented bioinformatics tools for molecular interpretation of the complex and information-rich data produced. Our goal is to address this bottleneck. We will develop algorithms for high-throughput putative annotation of hundreds of metabolites, knowledge-based downstream analysis, and validation of biologically-relevant leads. We will create the METASPACE engine, an open online platform providing these tools integrated into validated workflows for clinical use. This engine will be evaluated in clinical case studies on metabolic phenotyping of tumor response to chemotherapy and polymicrobial infections in cystic fibrosis. This demonstration will raise awareness and build trust among potential end-users. METASPACE will create a research ecosystem for exploitation of spatial metabolomic data that benefits both academics and industry. An open-source approach will stimulate developments in this field and provide a sustainable platform capable of incorporating future bioinformatics. Our user-centred tools, linked to existing molecular databases, will enable researchers without mass spectrometry or bioinformatics experience to turn big and complex HR imaging MS data into molecular knowledge. A considerable outreach effort, alongside constant interaction with the clinical metabolomics community, will maximize impact and dissemination. By engaging and educating envisaged end-users, METASPACE will facilitate
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Open-Source, Web-Based, Framework for Integrating Applications with Social Media Services and Personal Cloudlets' (OpenI), addressing dissemination and exploitation.

assistant: **Impact**

In today's society, accessing cloud-based services, such as YouTube, Spotify, Facebook, TripAdvisor, is predominantly through applications on mobile devices. Through everyday usage, consumers of these services provide these services providers with an immense amount of content and personal data. However, due to the lack of consumer-controlled cloud storage infrastructures and the siloed nature of cloud-based services, consumers are deprived of any control over their content or data. Furthermore, they are unable to make this data available to multiple applications across various services and devices – something which has led to a significantly impaired application usage experience and stagnation in application innovation.OPENi will define and deliver an open-source web platform that will enable mobile application consumers to store data and metadata from their mobile application usage in their own space in the cloud – the Cloudlet". This information can be shared (by and under the control of the consumer) securely among their applications, services and across their connected devices. To realize this vision OPENi will provide the required components that will support both consumers in creating and managing their Cloudlets but also Developers in creating applications that will be able to access and interact with them without disrupting service providers.OPENi will define and deliver the following prototype infrastructures:1.\tA cloud platform that will allow Users to instantiate, deploy and manage their own Cloudlet.2.\tAn API framework that will allow Developers to build applications that integrate dynamically a broader spectrum of cloud-based services and at the same time can access User Cloudlets to store and retrieve content and data.3.\tA set of value adding services,
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Development of strain and torque sensors based upon surface acoustic waves technology', explaining its relevance to EU policy priorities under Fifth Framework.

assistant: **Impact**

Surface acoustic waves (SAWs) occur at the interface of an elastic medium. The waves can be used to detect physical parameters at the surface such as strain, torque, pressure and temperature. The concept of SAW sensors has been proved at a scientific level but the industrial applications are very limited indeed. The potential for SAW sensors as a contact-less, low-cost and reliable sensor is enormous. This project will develop SAW sensors capable to operate for many different applications such as brushless DC motors, mixers for the food and chemical industry, electrical power tool, condition monitoring of rotating machines and parts, etc. The project will be an excellent solution for industries that at present cannot monitor their parts and/or processes due to lack of suitable sensors and will generate important markets within Europe.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Sensorimotor Integration, Motor Planning and Learning In FLY' (SIMPLIFLY) targeting Horizon Europe.

assistant: **Abstract**

We are constantly learning new motor skills throughout our life. Whether riding a bicycle or playing an instrument, motor learning relies on a calibration between sensory signals and internal motor information. Saccadic adaptation, the recalibration of fast eye rotations induced by visual error signals, has helped develop key insights into the identity of circuits, and the theoretical models underlying motor learning. However, how visual signals interact with internal movement representations during motor learning remains at present underdetermined. Taking advantage of the compact brain of the fly with well characterized visual pathways, and a plethora of anatomical, physiological, and genetic tools, here we propose to study motor learning in the context of goal-directed body saccades in D. melanogaster. We will monitor changes in body saccades consistent with repetitive visual perturbations by quantitative analysis of head-fixed flight behavior in an adapted version of the classical eye saccadic adaptation paradigm. In simultaneous, we will first record and then manipulate the activity of population of neurons known to contribute to body saccades, including visual descending neurons (VDNs) and their upstream synaptic partners, which as a network integrate visual signals and self-motion information with motor commands. Together, these experiments will establish unprecedented causal relationships among neural activity, learning, and the formation of a flexible internal representation for locomotor control. The functional principles identified in this project will establish a framework that can be tested in other species and behavioral contexts, and ultimately improve our understanding of motor learning with important implications both in health and disease.


**Keywords: