RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon 2020 proposal for the project 'Multi-Scale Fluid Flow in DYNamically FRACtured Porous Reservoir'.

assistant: **Objectives**

The unconventional recovery processes of oil and gas reservoirs raise significant costs and risks to the surrounding environment (including contamination of ground water, risks to air quality etc.) around the world and threatens to pose greater challenges in the future to meet the rising energy demand. The oil-gas industry is meeting this threat with developing diverse technologies that work by different mechanisms but share a common goal: to reduce risks for the surrounding environment and to increase the recovery factor of reservoirs. The aim of this project would be to develop a new fully coupled framework as an extension of research INTERSECT simulator in which we can model dynamic crack propagation with the injection of high velocity fluid flow as observed in the hydraulic fracturing (“fracking”) of unconventional reservoirs.


**Keywords:** fracking, meshless, enhanced and unconventional oil recovery, reservoir simulation
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Revealing the gene regulatory networks that govern cell mechanical properties by single cell microfluidics' to be submitted under Horizon Europe.

assistant: **Abstract**

Changes in mechanical properties of cells are key in a range of processes, including cell migration and development, and are frequently altered in disease states such as cancers. Yet, despite their key role, the gene regulatory networks underlying these processes are currently largely unresolved. Thus, the central aim of my proposed project is to gain a detailed understanding of how cellular mechanical properties are controlled, by developing microfluidic technology to simultaneously measure the mechanical phenotype and transcriptome of single cells in high throughput. The advent of single cell sequencing methods has been transformational for our understanding of biology, and multimodal approaches such as those combining genome and transcriptome measurements of the same cell, are likely to be even more so. The physical dimension, however, remains largely unexplored, and its exploitation offers the prospect of revealing how the biochemical composition of cells relates to their physical properties. I will thus apply my PhD experience to develop a microfluidic platform that combines physical and biochemical cell analysis, using real-time deformability cytometry and droplet-based single cell RNA sequencing. By matching the transcriptomic profile of each cell with its brightfield image, which yields their mechanical and morphological features, I will identify genes involved in the regulation of mechanical properties and their generality across cell types. In addition to elucidating fundamental regulators of cell mechanics, this technology will allow the investigation of their interplay with gene expression during both physiological and pathological cell state changes.


**Keywords:** Cell mechanics, microfluidics
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Sixth Framework proposal for the project 'USO-SP international graduate school for solar physics'.

assistant: **Objectives**

The USO-SP Consortium consists of Utrecht University (UU), the Royal Swedish Academy of Sciences (KVA) and the University of Oslo (UiO). Its mission is to advance solar physics. The present project starts an international graduate school in solar physics with training in a broad range of subjects, encompassing solar physics and stellar-atmosphere astronomy, advanced optics and polarimetry, wave-front restoration, large-volume image acquisition and processing, and numerical simulation techniques in radiation hydrodynamics and magnetohydrodynamics.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Lignin and Starch for Tinting Alternative Foods' under Horizon Europe.

assistant: **Concept**

The LISTAF project seeks to develop biodegradable photonic materials using starch and lignin as sustainable alternatives to conventional, non-biodegradable colorants in the food industry. These materials harness photonic color, which offers greater durability compared to pigment-based colors and finds applications across industries. The project centers on three key work packages:1. Precision production of starch nanoparticles (SNPs) through enzyme-assisted techniques, with a focus on controlling amylopectin branching and optimizing self-assembly conditions.2. Utilization of lignosulfonates and colloidal lignin as surfactants to stabilize SNPs, exploring variables such as concentration, pH, and addition timing to control SNP size and polydispersity.3. Development of photonic starch and coatings for food industry applications, including in vitro digestion and fermentation analyses to assess nutritional advantages.Outstanding challenges to address include overcoming SNP aggregation, gaining control over nanoscale starch-lignin interactions, and achieving close-packed assembly of the particles. The project aims to address these challenges through enzyme-aided research and innovative bio-based surfactant systems. Ultimately, the goal is to create eco-friendly, cost-effective, and nutritionally valuable materials that align with consumer demand for high-quality food products. The successful completion of this project will provide me with valuable training in lignin chemistry and bio-photonics, while also enabling me to conduct groundbreaking research in the field of starch applications. This comprehensive experience will expand my research expertise, yield high-quality publications, and significantly enhance my prospects for securing a faculty position in the future.


**Keyw
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'TArgeting Multiphosphorylated tau in Blood for non-invasive Alzheimer's Disease diagnosis and staging' (TAMBO-AD).

assistant: **Concept**

Alzheimer's disease (AD) is the major cause of dementia. The disease has an escalating impact on public health due to an increasing prevalence among the aging population in Europe and globally. In light of the first disease-modifying therapies becoming available, early diagnosis and precise disease staging are crucial for timely implementation of the treatment strategy. In TAMBO-AD, I will build on my preliminary findings to leverage AD biomarker potential of tau simultaneously phosphorylated at two distinct sites. By combining my expertise in immunoassay development with the top technology available at the host institution, I will develop three novel Single Molecule Array (Simoa®) assays targeting p-tau217&231, p-tau205&217, and p-tau205&231. Through cerebrospinal fluid and plasma measurements in the Amsterdam Dementia Cohort, I will evaluate performance of all three p-tau forms as: 1) early diagnostic and 2) disease-staging biomarkers. For the best-performing plasma biomarkers, I will translate Simoa® assays into innovative fiber optic nanogold-linked immunosorbent assay format (FONLISA), which enables point-of-care testing. New point-of-care testing assays will maximize biomarker testing accessibility, enabling use in primary care centers and in remote or resource-limited areas. Finally, I will communicate the project and disseminate results in English and Polish during an intersectoral secondment at the patient organization — Alzheimer Europe. The TAMBO-AD project represents a pivotal opportunity to advance my research, grow professional network, and gradually become an independent researcher.


**Keywords:** Alzheimer's disease,  biomarkers,  body fluid,  blood,  immunoassay,  clinical neurochemistry,  assay development,  phosphorylated tau
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Global Air TEMPerature ESTimation on high mountain glaciers' under Horizon 2020.

assistant: **Concept**

Glaciers provide a crucial water storage in high mountain environments and contribute to the seasonal freshwater availability for billions of people worldwide. Predicting the current freshwater contribution of mountain glaciers from model simulations is, however, limited by the lack of observational data and process understanding within inaccessible, high elevation regions. As such, glacier melt models make simplistic assumptions about meteorological information, namely air temperature, in space and time. The aim of this proposal is to produce a novel, unified approach for on-glacier air temperature estimation that can be implemented for any mountain glacier on earth by combining unprecedented meteorological data on glaciers with the latest advancements in gridded reanalysis information. The research fellow will combine dedicated field analyses on Alpine glaciers near to the host institution with existing, global datasets of air temperature data on glaciers, to produce a generalizable framework of glacier air temperature estimation that accounts for local interactions of cold and warm air masses and their causes. This framework will be developed and tested within detailed, high resolution land surface models at experimental catchments worldwide. The proposed research will exploit the resources, academic support and expertise of the world-renown supervisor and host institution to provide training to the fellow on model development and implementation for globally-applicable research and outreach deliverables. The scientific results are anticipated to have wide-reaching implications for current and future water resources management in high mountain regions and be disseminated through peer-reviewed publications, public outreach and open access data. The planned supervision,
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon 2020 proposal on 'Control of T cell differentiation and plasticity through mitochondrial reprogramming'.

assistant: **Innovation**

Mitochondria participate during the metabolic reprogramming of naive T cells. However, the molecular mechanisms by which mitochondria regulate T cell differentiation remain elusive. The project aims at revealing the mechanisms behind mitochondrial function and lineage specification and maintenance. Combining high-throughput analysis of gene expression and chromatin epigenetic status with biochemical, metabolic, cellular, and in vivo and in vitro approaches, we want to assess how mitochondria coordinate the metabolic status of the cell to transcriptional and epigenetic changes to control T cell differentiation and function in distinct inflammatory environments. For that our challenges are; (Obj.1) To investigate the role of mitochondrial dynamics in the metabolic reprogramming of T cell differentiation, (Obj.2) To study how metabolic pathways shape the transcriptional and epigenetic networks of the T cell lineages, (Obj.3) To identify mitochondria-to-nucleus signaling pathways that regulate T cell differentiation through modification of the transcriptional and epigenetic landscape, and (Obj.4) To investigate the therapeutic potential of reprogramming mitochondrial function in T cell responses against infection and cancer. REPROGRAMMIT will unveil significant breakthrough on (1) how mitochondria regulate the metabolic profiles of the distinct T cell subsets, (2) the identification of molecular candidates that reverse or modify T cell transcriptional programs through regulation of mitochondrial function, (3) the understanding on how nutrient availability and metabolic intermediates shape T cell differentiation and plasticity. In sum, REPROGRAMIT puts forward an ambitious and multidisciplinary but feasible program with the wide purpose of identifying novel checkpoints based
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon 2020 proposal for the project 'Disruptive fluorescent technologies for inflammatory bowel diseases'.

assistant: **Objectives**

Inflammatory bowel diseases (IBD) are chronic immune-mediated disorders causing inflammation of the gastrointestinal tract, which affect around 3 million patients in the EU. Current monitoring of IBD is mainly performed in the form of colonoscopies, biopsies or tests which are invasive or cannot specifically report on IBD activity. There is a need for new technologies that can rapidly measure activity biomarkers of IBD and enable the monitoring of disease progression and response to therapy accurately. This unmet patient need is what drives the development of this ERC PoC project.The ERC CoG DYNAFLUORS (Dynamic Activatable Fluorophores) project was funded to develop the first chemical toolbox for imaging the activity of immune cells in real time. In IBD, the activity of immune cells are important indicators of the disease state and can be used to assess how patients respond to therapy. DYNAFLUORS measure real-time immune cell activity; hence they are optimal tools to monitor disease progression in IBD patients facilitating a more accurate and personalised therapeutic approach. Based on our promising results in the ERC CoG, we aim to develop DYNAFLUORS into a new fluorescent technology for imaging IBD activity which will lead to: 1) new tools for IBD research and drug discovery in collaboration with companies covering the global fluorescent reagent market as well as Pharma, and 2) point-of-care technologies for clinical diagnostics in IBD patients.Funding from the ERC PoC will allow us to commercialise fluorescent reagents for research, in vitro diagnostics and drug discovery as well as to establish the foundations for new translational technologies to measure IBD activity in patients. This project will be guided by input from existing and new industrial and clinical par
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Vaccine profiling and immunodiagnostic discovery by high-throughput antibody repertoire analysis' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

Vaccines and immunodiagnostics have been vital for public health and medicine, however a quantitative molecular understanding of vaccine-induced antibody responses is lacking. Antibody research is currently going through a big-data driven revolution, largely due to progress in next-generation sequencing (NGS) and bioinformatic analysis of antibody repertoires. A main advantage of high-throughput antibody repertoire analysis is that it provides a wealth of quantitative information not possible with other classical methods of antibody analysis (i.e., serum titers); this information includes: clonal distribution and diversity, somatic hypermutation patterns, and lineage tracing. In preliminary work my group has established standardized methods for antibody repertoire NGS, including an experimental-bioinformatic pipeline for error and bias correction that enables highly accurate repertoire sequencing and analysis. The overall goal of this proposal will be to apply high-throughput antibody repertoire analysis for quantitative vaccine profiling and discovery of next-generation immunodiagnostics. Using mouse subunit vaccination as our model system, we will answer for the first time, a fundamental biological question within the context of antibody responses - what is the link between genotype (antibody repertoire) and phenotype (serum antibodies)? We will expand upon this approach for improved rational vaccine design by quantitatively determining the impact of a comprehensive set of subunit vaccination parameters on complete antibody landscapes. Finally, we will develop advanced bioinformatic methods to discover immunodiagnostics based on antibody repertoire sequences. In summary, this proposal lays the foundation for fundamentally new approaches in the quantitative analysis of
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Fifth Framework proposal on 'Physiological stress indicators in marine macrophytes'.

assistant: **Concept**

Marine macroalgae and seagrass communities are an important element of the world's coastal ecosystems. In eutrophicated areas, mats of green macroalgae, mainly consisting of Ulva spp., can often be observed. These mats have a strong negative impact on seagrass communities through competition for nutrients and light. To be able to assess and to predict the (in)stability of these systems there is a strong need to develop markers of physiological stress in marine plants.The proposal has the following research objectives:1) to develop stress indicators in macroalgae and seagrasses in mats;2) use these indicators to determine (physiological) acclimation mechanisms of algae and seagrasses in living in the intertidal zone. The project will combine field and laboratory studies. The study wil cover different scales, from the ecosystem level to physiological and biochemical approaches.Potential stress markers will be evaluated experimentally for responses to specific environmental stress variables in a restricted number of species and seasonal development of the markers will be monitored in the field. This proposal combines the use of traditional physiological analyses with more recently developed techniques, such as chlorophyll fluorescence. Additionally, the glutathione redox ratio and proteolytic activity will be determined, of which very little is known of in marine photosynthetic organisms. This proposal will be one of the first studies exploring these two markers in rnarine macrophytes.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'KD-ubiq - a blueprint for ubiquitous knowledge discovery systems', explaining its relevance to EU policy priorities under Sixth Framework.

assistant: **Impact**

This coordination action will bring together newly emerging research in ubiquitous knowledge discovery.Research areas are:- data mining in mobile systems, wireless communication networks, calm technologies- distributed architectures: distributed data mining, grid, P2P, autonomic computing,- agents- learning components: statistical learning (incl. online learning), evolutionary computing,- anytime algorithms data types: spatio-temporal, stream, multimedia- security and privacy: privacy preserving data mining, intrusion detection- HCI and cognitive modelling: user interfaces of ubiquitous discovery systemsThis multi-disciplinary approach constitutes a paradigm shift for the field of knowledge discovery since the idea of a standalone (desktop or workstation) analysis tool is abandoned in favour of process integrated, distributed and autonomous analysis systems. Work done in this area merely scratches the surface, is dispersed among several communities, and in a very early stage. Integration of the various sub-areas involves considerable risk. The CA KDubiq will act to close the gap and strengthen long-term research and applications in a new and future-oriented discipline ubiquitous knowledge discovery. It faces many new challenges, e.g. because of technical limitations in memory, CPU power, bandwidth etc, and can only succeed if privacy and security are addressed in a principled and multi-disciplinary manner.Objectives are to identify new research challenges, emerging from the changing nature of information and information sources: achieving the vision of ubiquitous knowledge discovery as a transparent set of tools, to be exploited everywhere, anytime, by all to derive strategic guidelines for long-term research by blueprint editing to structure and support emerging multi-