RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Dynamic proteomic maps of stem cell-derived neurons as a mechanistic discovery pipeline for rare neurological disease' in the context of a Horizon 2020 call.

assistant: **Objectives**

Rare diseases are a major unmet medical need, as is the definition of the relevant disease mechanisms. Many rare diseases affect the nervous system. These are challenging to treat, and mechanistic studies are difficult due to the inaccessibility of patient tissue. Global proteomic studies have provided insight into whole tissue or cell changes in protein abundance but lose information on protein subcellular localisation, which is important because defects in protein trafficking are implicated in many neurological disorders. In ‘RARE MAPS’ I propose an unbiased mechanistic discovery pipeline combining human induced pluripotent stem cells (hiPSCs) with advanced spatial proteomics. I will use a method developed by Dr. Borner called ‘dynamic organellar maps’, which provides quantitative protein subcellular localisation information at the whole proteome level. Used comparatively, it can detect changes in protein localisation due to a perturbation, allowing unbiased screening for phenotypic changes. To develop this workflow, I will apply it to the rare neurodegenerative disorder AP-4 deficiency syndrome. AP-4 knockout hiPSCs will be differentiated into cortical neurons and maps will be made of intermediate cortical stem cells and mature cortical neurons. Comparison to control cells will enable the detection of changes to protein localisation and abundance. I will also apply the maps to brain tissue from an AP-4 deficient mouse model to detect protein mislocalisation in vivo. I will then use CRISPR/Cas9 technology to investigate the role of novel and known AP-4-associated proteins in neuronal autophagy and axonal health. This project will demonstrate the utility of dynamic organellar maps to reveal molecular mechanisms of rare neurological disorders as well as provide new insi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Basal factor engineering and synthetic nucleosomal DNA super-template construction for pre-initiation complex assembly' under Seventh Framework.

assistant: **Concept**

Initiation of RNA polymerase II (Pol II) transcription is activated by the assembly of preinitiation complex (PIC) onto gene promoters. PIC contains the largest general transcription factor (GTF), TFIID that comprises TATA box-binding protein (TBP). The binding of TBP to TATA promoter is rate-limiting step in PIC assembly and is stabilized by GTFs, TFIIA and TFIIB. TFIID also contains reader domains that recognize specific epigenetic marks in nucleosomes located upstream and downstream of transcription start site. Structural information on the architecture of nucleosomes, DNA, TFIIA, TFIIB and TFIID in this context remains elusive to date limiting our understanding of DNA architecture at promoter site and the interaction of TFIID with epigenetically modified nucleosomes in transcription initiation. The objective of this proposal is to explore this cross-talk by creating a synthetic DNA super-template for PIC assembly containing modified nucleosomes, promoter DNA, TFIIA, TFIIB and TBP. This will be attempted via an international collaborative effort, employing integrated multidisciplinary approach including protein and DNA engineering and their supramolecular assembly combined with chemical cross-linking. This template will be analyzed with and without highly purified, fully recombinant TFIID (sub)complexes through a multi-resolution structural approach with X-ray crystallography, electron microscopy and small angle X-ray scattering techniques. This methodology will be useful to explore interactions of similar complexity involving other chromatin regulatory factors, thereby extending the horizon of transcription research or general multi-protein complex research, which is at the forefront of contemporary molecular biology. The interdisciplinary nature of this project pro
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Measuring co2 from space exploiting planned missions 2001-2004' under Fifth Framework.

assistant: **Concept**

To satisfy both scientific objectives and the political imperatives of climate negotiations, an integrated global observing system is required. We propose to develop and test algorithms to retrieve CO2 from satellite instruments. We will assess the accuracy and precision, and test the utility of the data for inferring surface CO2 exchange fluxes. Several instruments scheduled for deployment in the immediate future provide an opportunity to prototype the use of space-borne CO measurements. These instruments, IASI , SCIAMACHY and AIRS were not designed force measurements but as a result of their spectral characteristics provide capability, when used with independent measures of the temperature profile (from the microwave instrument AMSU). We will compare remote sensing and inverse approaches with approaches based on 4-dimensional data assimilation. Retrieved concentrations and fluxes will be evaluated against conventional observations.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Synergistic Integration of Hyperconducting Electric Propulsion and Composite Structures with Intelligent Morphing for Hydrogen-Powered Aviation' in the context of a Horizon Europe call.

assistant: **Objectives**

HyperMorpH focuses on hybrid-electric hydrogen-powered aircraft architectures. It suggests that as liquid hydrogen (LH2) becomes available for zero-emissions aircraft, cryogenically-cooled electric propulsion systems will become more economically viable. Advanced, sustainable composites, particularly advanced FRPs, will play a crucial role in this high-efficiency propulsion system due to their superior properties in demanding thermal and electromagnetic environments. HyperMorpH aims to develop a cryogenic ultra-light and high-power density hyperconductive electric motor using fibre-reinforced polymer (FRP) composites. Additionally, it will explore how this propulsion system can benefit from coupled aerostructure/propulsion systems integration, focusing on optimal aft-fuselage Boundary Layer Ingestion (BLI) configurations. The project will also investigate hybridization with under-the-wing H2 combustion turbine engines to enhance power supply during critical flight phases. To enable the HyperMorpH propulsion system concept, it will develop advanced composite materials, including self-morphing thermoplastic-based FRPs and metastructures, using sustainable and high-volume manufacturing techniques. Cutting-edge Artificial Intelligence (AI)-supported digital tools will facilitate multidisciplinary analysis and technology integration. The project will culminate in a laboratory-scale validation (TRL 4) of the integrated system, showcasing FRP-enabled cryocooled electric motors and next-generation self-morphing BLI propulsors, with the aim of achieving carbon neutrality in the aviation sector by 2050.


**Keywords:** aicraft, motor, hydrogen, advanced composites, efficiency, zero-emissions, BLI, cryogenic, electric, flight, digital tool, digital twin, intelligent agent, intelli
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon Europe proposal on 'Photodynamic Hydrogel for Encapsulated Release and Organoid-based Neu5Ac desialylation'.

assistant: **Concept**

Colorectal cancer (CRC) was estimated to be the second most commonly diagnosed cancer in Europe. CRC can be classified into subtypes based on distinct molecular and clinical features, termed consensus molecular subtypes (CMS 1-4). The proportion of CMS4 was 38.5% in CRC stage IV patients, only 6% of whom had a 5-year survival rate. CMS4 CRC is highly malignant and characterized by a richness of stromal fibroblasts and, consequently, an increased expression of mesenchymal genes. The sialylation of CRC stromal cells, as well as tumor cells and fibroblasts, has been verified as a key factor in forming the immunosuppressive tumor microenvironment (TME), which is also the main challenge for immunotherapy responses. Establishing multicellular CMS4 CRC spheroids helps unravel the mechanism of CRC TME and test the drug efficacy.3Fax-Peracetyl Neu5Ac (SI) is a sialyltransferase inhibitor capable of reversing sialylation in the TME. It can disrupt the immunosuppression of TME through desialylation and thus activate T cells and natural killer cells (NK cells). A growing body of evidence has proven that sialylation-included glyco-immune checkpoints are more widespread in tumor tissue and have a greater potential for application than PD-1/PD-L1 and CTLA-4 inhibitors currently in use. Notwithstanding, nephrotoxicity remains an unsolved problem in its application.PHERON aims to develop a photodynamic liposomes-encapsulated hydrogel to achieve the controlled release of SI at the colorectal tumor site, through a novel CRC multicellular spheroid model.


**Keywords:** immunotherapy, colorectal cancer, nanoparticle, hydrogel, desialylation, phototherapy, drug delivery, pharmaceutics, organoids, spheroids, nanomedicine
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Acceleration of the world’s first autotaxin inhibitor for the treatment of pancreatic cancer (PDAC)' (PDAC).

assistant: **Concept**

iOnctura is a clinical stage biopharmaceutical company dedicated to delivering innovative cancer treatments to patents with unmet medical needs. iOnctura’s unique pipeline of drugs unlock mechanisms at the tumour-stroma-immune (T-S-I) interface, a multi cell-network that is responsible for driving tumour growth and survival as well as treatment resistance.Pancreatic cancer is one of the most lethal cancers associated with devastating impact on the quality of life of patients; current treatments are largely ineffective and there is a need for game-changing therapies. IOA-289, is a first in class autotaxin (ATX) inhibitor that tackles cancer growth and spread by affecting core mechanisms at the T-S-I interface.The EIC Accelerator funding for CURE-PDAC will allow iOnctura to accelerate the clinical development of IOA-289 as a treatment for pancreatic cancer through Phase I/II clinical trials, reach a major value inflection point and attract further financial investment into the company.


**Keywords:** Health,Oncology,Drugs,pancreatic cancer,immunotherapy,fibrotic disease,clinical development,pharmaceutical industry
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Cloning and expression of genes involved in human senescence and longevity' in the context of a Fourth Framework call.

assistant: **Objectives**

Objectives:To clone genes linked with longevity.To clone genes linked with in vitro ageing.To make a comparative analysis of polymorphic markers of the cloned genes in samples of centenarians.To examine the role of ageing-accelerating and ageing-retarding agents in normal fibroblast cultures.To establish the biological role of the cloned genes by transfections and antisense micro-injections.Summary:The aim of this proposal is the identification and the study of the function of genes involved in the regulation of ageing and longevity. The four participating laboratories have complementary expertises in the field of molecular and cellular biology of ageing. One laboratory has already identified eight age-associated genes while the others have wide experience in gene and protein characterization, studies on centenarian cells and stress-induced accelerated ageing. The source material for the study will be human diploid fibroblasts either undergoing normal ageing or stress-induced accelerated ageing, and fibroblasts derived from centenarians.These cellular resources will be used for the isolation of eight specific and longevity-specific genes by three complementary methods:1) Suppression subtractive hybridisation will be applied to fibroblasts from centenarian versus in vitro normally aged fibroblasts from young subjects in order to identify genes associated with longevity;2) Differential display will be used to clone genes from fibroblasts undergoing stress-induced accelerated ageing in comparison with unstressed in vitro aged fibroblasts at the same passage number;3) Phage display technology for cloning genes on the basis of differential protein expression in human diploid fibroblasts undergoing in vitro ageing. In control experiments the specific expression of the isolate
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Competition in the Digital Era: Technology and Geopolitics in the XXI Century' under Horizon Europe.

assistant: **Concept**

The Competition in the Digital Era (CODE) project investigates the competition between China, Europe and the United States (US) for access and control of three critical technological sectors: semiconductors, cloud computing and space. These sectors are, respectively, the backbone, the brain and the legs of the current wave of technological innovation and they have a fundamental impact on economic, military and geopolitical hierarchies.CODE proposes a multidisciplinary theoretical framework and an original mixed-method approach to study technology competition in these sectors. The main hypothesis of the project is that the very technological characteristics of the industrial sectors are key to explain when and why China, Europe and the US succeed in some sectors and struggle in others. Specifically, different combinations of cycle of technological change (the speed at which technologies evolve in each industrial sector) and the degree of modularity (the possibility to divide complex technical systems into components and outsource them to external suppliers) generate different hypotheses on the ability of China and Europe to compete with the US in semiconductors, cloud and space.CODE is a high-risk project because investigates technologically complex and highly politicized sectors. Yet, it is also a high-gain project as simultaneously advances the debate on international relations (US-China geopolitical transition, Europes role in the world), political economy (role of the state in technology innovation, role of global value chains) and management and innovation (how technology competition unfolds in highly politicized sectors).


**Keywords:** Geopolitics,  Technology,  China,  Europe,  Political Economy,  Semiconductors,  Cloud Computing,  Space,  Competition
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'Surface Plasmon-Based Wifi for Nanoscale Optical Information Transport - SWIFT'.

assistant: **Objectives**

This proposal focuses on the design, fabrication, characterization and optimization of novel groundbreaking communication nano-devices. SWIFT proposes resolutely innovative concepts adopting metal-based optical nano-antennas as a disruptive technological vehicle. Nanoscale electronics and photonics exploit novel fascinating physical phenomena and are among the most promising research areas for providing functional nano-components for data transfer and processing. The aim of this proposal is to interface these two device-generating technologies to create the first electrically-driven nanoscale optical antenna transceiver. The concept will enable electron/photon transduction at the nanoscale by a unique surface plasmon-assisted metal-based design, a significant leap at the forefront of research in nanoelectronics and nanophotonics. SWIFT proposes a series of fundamental advances motivated by application-driven perspectives that will push the burgeoning field of optical antenna to a new area. Deploying optical antenna transceivers enables a paradigm shift in optical interconnects and communication at ultimate device densities through the following innovations:• Development a whole new class of plasmon-assisted transducing optical functional nanodevices.This unique concept addresses the development for ultracompact nanocomponents.• Prototyping self-sustained plasmonic in/out electrical ports on SPP waveguiding platforms, removing thus complex optical interfacing that cannot be miniaturized.• Pioneering a technological breakthrough enabling nanoscale wireless broadcasting of optical information.• Using these functionalities, we will prospect new research directions by proviing a unique ground for (i)  generating ultrafast electron surges in an integrated electronic layout en
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Micromachined SOI Digital Light Switch with Integrated CMOS Electronics' (MICROSLICE), addressing dissemination and exploitation.

assistant: **Impact**

Over the past decade, telecom network systems have increased dramatically in capacity/speed due to rapid developments in technologies such as Micro-Optical-Mechanical Systems (MOEMs). New systems have emerged, which include AWG's (array wave-guide gratings) for Wavelength Division Multiplexing (WDM) and OADM (Optical-Add-Drop-Multiplexers) for signal routing. However, as these systems evolve, there is a need for higher levels of functional integration of the mechanical/optical elements with their drive/sense electronic circuitry in order to decrease assembly costs and improve system performance. In this project, a novel optical transmission switch will be fabricated and the feasibility of monolithic integrating 'on-chip' both the mechanical actuating elements of the switch with it's CMOS drive circuitry will be assessed using Silicon-on-Insulator (SOI) technology. The use of SOI, enables 'on-chip' electronic integration with greater functionality and performance for next generation system designs. Over the past decade, telecom network systems have increased dramatically in capacity/speed due to rapid developments in technologies such as Micro-Optical-Mechanical Systems (MOEMs). New systems have emerged, which include AWG's (array wave-guide gratings) for Wavelength Division Multiplexing (WDM) and OADM (Optical-Add-Drop-Multiplexers) for signal routing. However, as these systems evolve, there is a need for higher levels of functional integration of the mechanical/optical elements with their drive/sense electronic circuitry in order to decrease assembly costs and improve system performance. In this project, a novel optical transmission switch will be fabricated and the feasibility of monolithic integrating 'on-chip' both the mechanical actuating elements of the switch wit
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Fourth Framework proposal on 'Biodegradable polyalkenoate cements'.

assistant: **Concept**

The objective of this project is to look at the feasibility of developing a biodegradable bone glue for fracture fixation in orthopaedic surgery. Recently glass polyalkenoate cements formed from poly(acrylic acid) and ion leachable glasses have been developed as chemically adhesive bone cements. However there is a clinical need for a degradable adhesive. The objective of the current project is to synthesise and characterise poly(B Malic Acid). Poly(B Malic Acid) has the same chemical structure as poly(acrylic acid), but has an ester group in the polymer backbone and will hydrolyse to Malic Acid a metabolite of the TCA cycle. The chemistry of cement formation between Poly(B Malic Acid) anci ion leachable fluorosilicate glasse subsequently will be investigated and te mechanical properties of suitable cements characterised.