RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Artificial RNA regulators to probe, control, and design gene regulatory networks in bacteria' to be submitted under Horizon Europe.

assistant: **Abstract**

RNA is common to all organisms. Despite its major function as the coding agent for protein synthesis, an increasing number of regulatory roles have been assigned to RNA. In bacteria, small RNAs (sRNAs) constitute the best-studied class of non-coding regulators estimated to control ~20% of all genes in a given organism. Most sRNAs affect gene expression by base-pairing with multiple target mRNAs resulting in either gene repression or activation. sRNA regulators are modular, versatile, and highly programmable, and thus have gathered momentum as control devices in synthetic biology and biotechnology.My group has recently established artificial sRNAs as a potent genetic tool to screen, detect, and characterize microbial phenotypes. We have now extended this method by a novel sequencing approach, called LIGseq, allowing us to map sRNA-target interactions at the population level and in a high throughput manner. We have further shown that sRNAs expressed from the 3’ untranslated regions (UTRs) of mRNAs can serve as tuneable autoregulatory elements and thus bear ample possibilities for the design of artificial gene networks. I therefore posit that artificial sRNAs are powerful, yet understudied control elements of the synthetic biology toolbox with largely untapped regulatory potentials.  I thus propose to: 1) exploit artificial sRNAs to investigate the molecular principles underlying target selection and RNA duplex formation by bacterial non-coding RNAs, 2) harness the power of artificial sRNAs to study essential gene functions and the mechanisms underlying antibiotic resistance in bacteria, 3) employ 3’UTR-derived sRNAs as programmable feedback devices in synthetic gene regulatory circuits.  This combined work will generate the molecular framework to employ artificial sRNAs f
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'A bone marrow mimicry bioreactor for cost effective production of hematopoietic stem cells' (MarrowMimi), addressing dissemination and exploitation.

assistant: **Impact**

Hematopoietic stem/progenitor cell (HSPC) therapies have the potential to treat over 70 diseases including blood cancers and leukemia and are one of only 22 advanced therapy medicinal products currently approved by the European Medicines Agency. However, the number of HSPCs derived from a single blood donation can be insufficient for modern therapies, which typically require a minimum of 10^7 cells. To service adequate treatment regimes, HSPCs must be expanded in the laboratory at great financial expense, resulting in exorbitant treatment costs of up to 315,000. New methods of ex vivo HSPC expansion are therefore required to reduce production costs and ensure therapy affordability.The industry standard for HSPC expansion utilises liquid suspension bioreactors, where cells are diluted in litres of culture media to a final concentration of only 10^3-5 cells/mL. This liquid media, containing large amounts of expensive nutrients, provides high cell expansion but results in high production costs. Liquid suspension bioreactors vary significantly from how humans grow HSPCs within high-cell-density tissue (10^8-11 cells/mL), which require only a fraction of the nutrient quantities per-cell.The bone marrow is the human bodys natural HSPC bioreactor and provides a defined microenvironment for optimal cell production. I aim to artificially replicate these niche conditions by creating a marrow mimicry bioreactor (MarrowMimi) to maximise ex vivo HSPC expansion by (1) formulating a hydrogel scaffold to mimic the bone marrow extracellular matrix, (2) utilising state-of-the-art 3D printing technologies to create a marrow-like perfusion bioreactor, and (3) optimising reaction conditions to maximise HSPC expansion and minimise nutrient demand. The ability to minimise HSPC expansion-assoc
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Advanced III-V Materials and Processes Enabling Ultrahigh-efficiency ( 50%) Photovoltaics' (AMETIST), addressing dissemination and exploitation.

assistant: **Impact**

Compound semiconductor solar cells are providing the highest photovoltaic conversion efficiency, yet their performance lacks far behind the theoretical potential. This is a position we will challenge by engineering advanced III-V optoelectronics materials and heterostructures for better utilization of the solar spectrum, enabling efficiencies approaching practical limits. The work is strongly motivated by the global need for renewable energy sources. To this end, AMETIST framework is based on three vectors of excellence in: i) material science and epitaxial processes, ii) advanced solar cells exploiting nanophotonics concepts, and iii) new device fabrication technologies. Novel heterostructures (e.g. GaInNAsSb, GaNAsBi), providing absorption in a broad spectral range from 0.7 eV to 1.4 eV, will be synthesized and monolithically integrated in tandem cells with up to 8-junctions. Nanophotonic methods for light-trapping, spectral and spatial control of solar radiation will be developed to further enhance the absorption. To ensure a high long-term impact, the project will validate the use of state-of-the-art molecular-beam-epitaxy processes for fabrication of economically viable ultra-high efficiency solar cells. The ultimate efficiency target is to reach a level of 55%. This would enable to generate renewable/ecological/sustainable energy at a levelized production cost below ~7 ¢/kWh, comparable or cheaper than fossil fuels. The work will also bring a new breath of developments for more efficient space photovoltaic systems. AMETIST will leverage the leading position of the applicant in topical technology areas relevant for the project (i.e. epitaxy of III-N/Bi-V alloys and key achievements concerning GaInNAsSb-based tandem solar cells). Thus it renders a unique opportunity
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'MAS DE NADA: Modeling and Advanced Software Development for Electrical Networks in Aeronautical Domain Analysis' (2007-2013).

assistant: **Concept**

The main objective of the current proposal is the development of an Electrical Network Analysis Model (ENAM) in Saber simulation environment and at behavioral modeling level. The model will be used for the analysis of power quality, stability and reliability of different configurations for the Electrical Test Bench (ETB). The proposal is based on the following keypoints:1) a detailed Saber model for the ENAM will be implemented in order to perform short transient analyses. Starting from the literature regarding electrical equipment modeling, first a mathematical model will be derived. Successively, it will be translated into an electrical scheme adopting Saber standard library elements where possible, or user-defined components implemented in MAST code as an alternative. The ENAM will be equipped with a high-level supervisory control scheme, in order to manage failures. About testing process, a set of devoted tests will be performed, mainly referring to required stability, power quality, reliability and failure mode behavior.2)a devoted software will be implemented in order to interface with the Saber models and perform the required tests. The software will be implemented as standalone, however requiring the presence of a full Saber package installed on the target machine, but hiding to the user the complexity of the Saber environment. The devoted software will use an external mathematical calculation environment (e.g. Matlab) for implementing the post-processing functionalities above reported (i.e. power quality, failure mode and reliability analysis)The proposed Project follows a typical workflow for both modeling process and software development basic tasks.The modeling activities will be based on main theoretical analysis, mathematical formulation, models implementa
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Development of an innovative and cost-efficient friction and wear reduction solution (TriboGlide)' (TriboGlide).

assistant: **Concept**

Friction and wear are encountered in all areas with relative motion between contacting surfaces. The resulting energy losses are huge: In economic terms friction and wear of materials are estimated to amount 5–8 % of an industrialized country’s GDP . Conventional solutions to reduce friction and wear include polishing, Diamond Like Carbons coatings (DLC), phosphating, bronze (bushings and pins) and plasma spraying. Applied Nano Surfaces’ (ANS) unique patented offering Triboconditioning® has been proved in over 70 customer projects to be a very high performant (achieving a reduction of friction up to 70% and wear up to 90%) and cost efficient solution in many industrial applications such as engines, compressors, stirling engines, pumps, hydraulic systems and various bushings and bearings. The cost of our technology is substantially lower than what our competitors can offer (up to 8100%) and it requires significantly lower investments in production. We are working with most of the major brands and companies (major auto brands/OEM and manufacturers like e.g. Mahle, GnuttiCarlo, Caterpillar, Daimler) that are continuously making efforts to increase fuel efficiency and increase performance of their products. The already signed license agreements are proof that we have an attractive solution to offer our customers.ANS has a core competence and expertise in the field of tribology, low friction coatings and international business development. The business model is based on a four-step customer approach that lets customers test the product in their own premises and then adjust it to the specific customer needs finalizing in license deals. The market potential for ANS’ technology is substantial and increasing. All mechanical systems in the world waste useful kinetic energy due to
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Mai-water, multi analyte immunoassays for detection of pesticides in water' under Fourth Framework.

assistant: **Concept**

Pesticides are used to control or eliminate pests and diseases occurring in agriculture, food storage and so on. They are therefore present in many kinds of environment, such as surface water. Pesticides are toxic for humans and animals, and consequently (international) standards and regulations safeguard our environment (EC Directive 80/778/EEC, 1980). If the concentration of pesticides remains too high EC regulations stipulate that drinking water companies stop taking up surface water and stop supplying drinking water. Water companies therefore continually monitor the degree of pesticide contamination in surface and drinking water. Conventional and recognised analysis techniques only measure the presence and quantity of one pesticide, are expensive (400 ECU per analysis) and time consuming (about 2 days per analysis). Because of this, the number of analyses performed is limited and optimal quality control is nearly impossible. Other industries such as food processing, veterinary science, pharmacy and biochemistry have similar problems. The main objective of the MAI WATER project is to RTD Multi Analyte Immunoassay (MAI) systems for the simultaneous detection and measurement of multiple pesticides in surface water.Compared to conventional analysing methods MAI will (1) provide simultaneous analysis of several pesticides, (2) be easy to use in Lab and Field, (3) reduce costs by 80 90%, (4) detecting less than 0.1 ?g/L (EC Norm) and (5) analyse 5 20 times faster.The research objectives are (1) RTD of simultaneous detection of several pesticides, (2) RTD of a MAI Bio Sensor field system, (3) RTD of a new fluorescent, signal producing compound (tracer) and (4) MAI Lab and MAI Field System prototypes development and testing. Novelty Verification (literature, patents, end us
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Dissection of centromeric chromatin and components: A biosynthetic approach' (BIOSYNCEN) under Seventh Framework.

assistant: **Objectives**

The centromere is one of the most important chromosomal elements. It is required for proper chromosome segregation in mitosis and meiosis and readily recognizable as the primary constriction of mitotic chromosomes. Proper centromere function is essential to ensure genome stability; therefore understanding centromere identity is directly relevant to cancer biology and gene therapy. How centromeres are established and maintained is however still an open question in the field. In most organisms this appears to be regulated by an epigenetic mechanism. The key candidate for such an epigenetic mark is CENH3 (CENP-A in mammals, CID in Drosophila), a centromere-specific histone H3 variant that is essential for centromere function and exclusively found in the nucleosomes of centromeric chromatin. Using a biosynthetic approach of force-targeting CENH3 in Drosophila to non-centromeric DNA, we were able to induce centromere function and demonstrate that CENH3 is sufficient to determine centromere identity. Here we propose to move this experimental setup across evolutionary boundaries into human cells to develop improved human artificial chromosomes (HACs). We will make further use of this unique setup to dissect the function of targeted CENH3 both in Drosophila and human cells. Contributing centromeric components and histone modifications of centromeric chromatin will be characterized in detail by mass spectroscopy in Drosophila. Finally we are proposing to develop a technique that allows high-resolution mapping of proteins on repetitive DNA to help further characterizing known and novel centromere components. This will be achieved by combining two independently established techniques: DNA methylation and DNA fiber combing. This ambitious proposal will significantly advance our und
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Systems biology towards novel chronic kidney disease diagnosis and treatment' (SYSKID).

assistant: **Concept**

Chronic kidney disease (CKD) affects up to 10% of the population. Besides eventual progression towards end stage renal disease CKD impacts the patient’s quality of life by causing serious comorbidities including cardiovascular complications and bone metabolism disorders. On the everyday clinical level early stage diagnosis and tailored treatment of CKD are still inadequate. In addition, CKD seems not to have reached its appropriate emplacement in an epidemiological and healthcare perspective yet, and the pathophysiology of the disease on a molecular and cellular level is not well enough understood. Our sysKID consortium was installed for precisely addressing these issues: To unravel the molecular and cellular mechanisms of chronic kidney disease development, combine this information with clinical risk factors, and on this basis delineate chronic kidney disease biomarkers. These markers will allow us to perform preclinical studies of novel therapy approaches for halting disease progression, and will provide us with the materials for development and clinical evaluation of tools for early stage diagnosis as well as prognosis and treatment monitoring. sysKID assures a successful implementation of these goals by a truly international consortium of 27 leading research groups. We combine clinical know how, provide access to a huge chronic kidney disease sample and clinical data pool, and build a Systems Biology framework for chronic kidney disease by integrating molecular and cellular biology, computational biology, statistics and epidemiology. Our expert group is further complemented by a high level advisory board covering science, product development, and the patient’s perspective. sysKID implementation is structured for completing pre-clinical Proof of Concept studies of no
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Nitrogen fixation in agriculture and the environment: complementary research between the eu and developing countries' (NIFEED) under Fifth Framework.

assistant: **Objectives**

40 young researchers from the EU & developing countries will be invited to the 5th European Nitrogen Fixation Conference being held in Norwich, UK, on 6-10 September 2002. Half will be from EU-funded projects and the rest from EU target areas, selected by open competition. This will enlarge the community of researchers in nitrogen fixation from developing countries and will also address the interests of European scientists pursuing research in low input agriculture. To meet their particular needs, emphasis will be placed on the legume/rhizobial symbiosis. A special oral and poster session at the Conference will allow the selected participants to present their results, abstracts of which will be included in the Conference booklet. A novel feature will be a workshop to set up a website to enable exchange of data, methods & other information which will be linked to the Commission and other major organisations such as FAG and Cigars.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'The History of Feminist Political Thought and Women’s Rights Discourses in East Central Europe 1929 - 2001' in the context of a Horizon Europe call.

assistant: **Objectives**

This project will produce the first comprehensive and multi-disciplinary history of feminist political thought and women’s rights discourses in East Central Europe in a broad chronological perspective. The project builds on a solid base of existing scholarship, but does more than weave the stories of individual women or fragmented national case studies in the existing scholarship into a coherent narrative. Instead, it reinterprets the story of feminism in the region in relation to state socialism and the history of state socialism through the lens of feminism. It treats the interwar pre-history of debates between socialism and feminism, as well as the post-1989 struggles to deal with the state socialist past as integral parts of the history of state socialism. Intellectual historians have paid relatively little attention to this region, to women, and to feminism. Yet it is precisely the issues traditionally at the heart of their field – such as the origins and transformations of ideas in their social and cultural contexts – that are required to understand feminism in East Central Europe. Recent advances in the field have often been driven by methodological innovations, and particularly the use of sources beyond political essays and academic publications. This project also draws upon sources like popular women’s magazines, literary and art works, samizdat publications, party documents, and oral history interviews. The research team reflects the diversity of the European continent, mobilising linguistic and methodological skills to match the project’s broad source base, inclusive geography, and extended chronology and to produce a body of research that is both comprehensive in scope and sensitive to local particularities. By situating them in their local, regional and tra