RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Constructing Solidarities. Kinship Ties and Social Networks in the Urban Communities of Italy and the Low Countries, 1250-1550""' (MCEUICS), addressing dissemination and exploitation.

assistant: **Impact**

In the rapidly growing cities of late medieval and early modern Italy and the Low Countries new forms of trust emerged that gave social cohesion to the urban community. The new trust networks, based on the building blocks of kinship ties and extra-familial co-operation, strengthened the processes of social integration and community building in the medieval city. This project aims to reveal the social mechanisms that underlay these solidarities as well as to determine the factors that facilitated their formation. It does so by comparing the developments in cities with different political and socio-economic profiles from Italy and the Low Countries. This comparative research will enhance our knowledge about the structural factors that explain the relation between the emergence of trust networks – in particular the ruling elites and the guild organisations – and the evolution of urban communities and civil society. This approach raises methodological challenges concerning the comparison of different case studies and diverse data, which must be dealt with. The research will also contribute to the interdisciplinary debate about the practice of trust and the functioning of familial and extra-familial solidarity, drawing on sociological theory pertaining to trust and social networks. Finally, the research will add to our understanding of the bottom-up trust-building strategies of actors of various political and socio-economic backgrounds, thereby assessing the impact of more general forms of trust embodied by the medieval urban corporate and public institutions. The central question, however, is to explain how kinship ties and extra-familial social networks strengthened and facilitated the process of urban community building in Western Europe from 1250 to 1550.""
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'DNA based nanometry: Exploring chromatin structure and molecular motors' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

DNA metabolism is governed by a delicate balance between compacting the stored genetic information while simultaneously ensuring a highly dynamically access to it. This interdisciplinary project aims (i) to understand the mechanics and dynamics of chromatin as well as the mechanism of enzymes involved in DNA metabolism on a molecular level and (ii) to develop new nanometric tools based on optical methods and 3D DNA nanostructures that allow addressing new experimental questions. Within the research project novel nanoscopic detection assays based on the combination of magnetic tweezers and optical methods shall be developed, such as ultra-fast torque spectroscopy and combined FRET-force spectroscopy. Our single-molecule assays shall be applied to study the material properties of self-assembled 3D DNA nanostructures, which shall then be used to set up improved high resolution single-molecule assays. These technological improvements will become key to obtain insight into structure and dynamics of in vitro reconstituted chromatin as response to external mechanical stress but also into the operation of molecular motors that themselves generate forces and torques on DNA and chromatin. The main goal of the project is to use nanotechnological tools to understand design principles of biomolecules, biomaterials and biological motors, which in turn shall be used to develop smarter nanotools and functional elements.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'Novel Biocatalysts for the Production of Glycosides'.

assistant: **Objectives**

This project aims to develop novel biocatalysts for the production of glycosides (NOVOSIDES). Glycosylated compounds have a wide range of applications, but very few enzymes are able to glycosylate small organic molecules cost-efficiently at the industrial scale. Therefore, glycosylation reactions catalysed by transglycosidases, glycoside phosphorylases and glycoside hydrolases will be explored in more detail. These enzymes catalyze the transfer a glycosyl group from a cheap and readily available donor substrate to a variety of acceptors. To exploit their full potential, the enzymes’ specificity and stability against high temperatures and the presence of organic co-solvents will be optimised by means of directed evolution.A large and diverse collection of enzymes will first be established by screening in natural environments and by the mining of public (meta)genome databases. The enzyme collection will then be screened for activity on a variety of representative acceptors from different chemical classes. This will allow the identification of the most promising enzymes for optimisation through semi-rational and random mutagenesis. The high-throughput screening of natural and variant enzymes will be performed with newly developed fluorescent probes, that allow fast and accurate measurements of carbohydrate-active enzymes in a direct and non-destructive assay.To achieve these ambitious goals, a complementary consortium of academia and industry has been formed that covers the whole range of required expertises. The economical potential of our technology will be demonstrated by the development and scale-up of selected glycosylation reactions at pilot-plant facilities. The produced glycosides will be actively marketed to potential end-users to promote the valorisation of the p
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'PanCare Studies in Fertility and Ototoxicity to Improve Quality of Life after Cancer during Childhood,  Adolescence and Young Adulthood' in the context of a Seventh Framework call.

assistant: **Objectives**

Survival rates after childhood cancer now reach nearly 80% in developed European countries as a result of more effective therapies and better supportive care, leading to a steady increase in the number of survivors in the population. However, the treatments that have improved survival are harsh and cause serious side-effects that can greatly impact survivors’ quality of life in the long term. The goal of PanCareLIFE is that survivors of cancer diagnosed before age 25 should enjoy the same quality of life and opportunities as their peers who have not had cancer. Using observational studies and molecular genetic investigations PanCareLIFE will investigate late effects that impact fertility and hearing impairment (ototoxicity), and will assess health-related quality of life. Information from PanCareLIFE’s studies will be incorporated into new guidelines for fertility preservation. As the number of survivors with late effects in any one country is small, large cohorts are required for accurate estimation of risk. PanCareLIFE has assembled a team of prominent investigators from 8 European countries who will contribute in total over 12,000 well-characterised research subjects to identify risk factors, both genetic and non-genetic, linked to decrements in fertility and ototoxicity. Quality-of-life studies will evaluate the impact of fertility and ototoxicity. PanCareLIFE will advance the state-of-the-art in survivorship studies by evaluation of large cohorts with observational and genetic tools that will provide better knowledge of individual risk factors. Survivors can then be stratified into groups benefitting from personalized, evidence-based, care; future patients may expect effective therapies to have less severe side effects, and plans for a seamless transition to long-t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Potato crop effective management strategies to tackle future pest threats' will create measurable scientific, societal and economic impact aligned with Horizon Europe.

assistant: **Impact**

Potato is a key crop in Europe (€12.4 billion, 2020). However, it is considered vulnerable to a variety of pests and soil-borne pathogen diseases. One of the most important pests is caused by bacteria Candidatus Liberibacter solanacearum (CLso), causing a disease known as zebra chip (ZC). The introduction of CLso bacterium on potato is mainly linked to the presence of the vector psyllid Bactericera cockerelli (BC) causing severe damage, both in terms of yield losses and quality. Main potato postharvest diseases as dry rot, black dot and silver scurf, are caused by soil-borne pathogens that have incidence in both field and storage. All these potential threats may result. in significant economic losses in the potato industry and food losses. The European regulatory framework for potato’s imports and production is very strict. However, globalisation, climate change, economical drivers, as well as the political context (EU Green Deal target of 50% pesticides reduction) are threatening the entrance of emerging potato pests and the incidence of soil-borne pathogen disease in postharvest storage. PataFEST and its 18 complementary partners, including the international cooperation with Ecuador, aims: 1) to characterise at molecular level the ecological pest spread pathway and identify potato disease resistance varieties against CLso and postharvest pathogens, 2) provide effective preharvest plant and soil treatments against CLso vector and soil-borne pathogens combined with other cutting-edge digital technologies such as image analysis tools (mobile app) and artificial intelligence predictive models; 3) develop postharvest technologies (biocontrol coating solution, controlled atmosphere storage and volatile organic compounds (VOCs) sensors) to control the incidence of soil patho
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Improving surface and bulk properties of promising fusion material using deep cryogenic treatment' demonstrating excellence, impact and implementation quality for a Horizon Europe call.

assistant: **Abstract**

With progress and development of society, the necessity of a more affordable, reliable and sustainable energy is crucial to stability and prosperity of ever-growing population. For this reason, fusion power is being researched and developed to obtain high amounts of stable and greener energy compared to conventionally fossil fuel-based and fission processes. Materials, which are being tested as possible candidates for fusion reactor components, are mainly based on the previous selections designated for fission nuclear power. However, their implementation is not easily transmittable and has been proven to be considerably challenging. Consequently, there is a high interest towards research of new development and processing (such as heat treatment) of selected materials applicable for fusion as well as materials that were not previously considered. The proposal suggests to conduct fundamental research on selected material (proposed alternative AISI 431) and its treatment, which has the potential to be used as material for fusion reactor components. The project tends to provide base understanding on how deep cryogenic treatment (DCT) improves materials’ properties for fusion applications through their microstructural tailoring. Focus is placed on the role of modified microstructure as well as nano-chemistry on the corrosion resistance, which is up to now only superficially explored. Due to the unique influence of DCT on microstructure, the process can provide a novel greener way of material treatment that is both highly effective as well as economically feasible. The proposal challenges promising alternative material for fusion and aims to show how DCT can effectively improve microstructure, corrosion and surface resistance of selected material. Furthermore, the project wil
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Multi-dimensional mapping of lineage specific transcription factors through time and space' (GRADMAP), addressing dissemination and exploitation.

assistant: **Impact**

Here I propose an ambitious project designed to explore an exciting new hypothesis: TFs are non-randomly spatially organised within the cell nucleus, forming radial gradients that likely play an important role in regulating gene expression.  This idea was born from one of the intriguing discoveries revealed by Genomic loci Positioning by Sequencing (GPSeq), a technique developed in the host lab, that the binding motifs of the majority of human transcription factors (TFs) seem to form radial density gradients, with some TF binding motifs being more abundant towards the nuclear periphery, whilst others more abundant in the centre. Furthermore, preliminary analysis showed that the predicted binding motifs of master regulator TFs specifying lymphoid vs myeloid lineages form opposing radial gradients, suggesting that this mode of transcriptional regulation is critical for cell fate specification. In this project, I will differentiate hematopoietic stem cell (HSC) into opposing lineages (lymphoid/myeloid) and perform TF CUT&RUN to assay TF occupancy and chromatin landscape, coupled with GPSeq to assay the radial arrangement of chromatin in these lineages. The intersection of these two genome-wide datasets will provide the first ever map of the occupancy of lineage specific transcription factors through differentiation time and nuclear space with respect to radiality.  In parallel I will generate additional omics datasets in these cell populations to assay chromatin accessibility, transcription and chromatin interactions to build a comprehensive, multi-dimensional genomic atlas in which the spatial arrangement of TFs can be linked with gene expression dynamics. Crucially, no other study has thus far addressed this novel hypothesis; my work will provide significant advancements
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Developing next-generation tools for mitochondrial dissection with cell-specific resolution.', explaining its relevance to EU policy priorities under Horizon 2020.

assistant: **Impact**

Mitochondria generate most of the energy cells require to function. Deficits in the mitochondrial energy-generating machinery affect 1:5,000 children and cause progressive, debilitating, and usually fatal pathologies collectively known as primary mitochondrial disease. To date, there is no cure for mitochondrial disease and existing treatments are highly ineffective and mostly palliative. High-energy-requiring cells, such as neurons, are especially affected in mitochondrial disease.  However, not all neuronal populations are equally affected. Furthermore, the molecular determinants of neuronal vulnerability to mitochondrial disease have not been adequately elucidated, representing a challenge for the development of efficient treatments for these pathologies. To improve on current knowledge on mitochondrial disease and to provide better therapeutic targets, this proposal focuses on developing ground-breaking molecular biology tools that will allow the identification and dissection of the molecular determinants of neuronal vulnerability in mitochondrial disease with unprecedented definition. I will develop novel techniques to isolate the mitochondrial translatome by using ribosomal tagging, as well as to assess intact mitochondrial function, with cell-type specificity. These novel approaches will have a high impact in mitochondrial disease research, with the overall aim of identifying novel therapeutic targets that will lead to effective treatments for mitochondrial disease. Furthermore, the high applicability of the tools generated will allow significant breakthroughs in the research of other pathologies with mitochondrial affectation such as diabetes or neurodegenerative processes.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon 2020 proposal on 'JobCity: addressing global youth unemployment by revolutionising psychometrics to enable Multiple Mass Psychometric Testing (MMPT) through online gamification'.

assistant: **Innovation**

Linkko Partners Ltd aims to develop a disruptive technology to revolutionise employment psychometrics by enabling Multiple Mass Psychometric Testing (MMPT) through gamification, with particular attention to the worldwide problem of youth unemployment.The key objectives of the Project:• Create Multiple Mass Psychometric Tests delivered through an accessible online game• Develop multi-platform, youth-friendly software for users to access in their own environment• Develop language packs to allow a single core product version rollout in multiple countries • Report on Aptitudes, Skills, Abilities & Preferences (ASAP), creating a Profile in minutes• Deliver results in a fraction of the time of paper tests and at a fraction of the cost• Develop a robust scalable cloud infrastructure to allow 1,000s of simultaneous tests & evaluations • Identify barriers to employment for non-academic/low qualification jobseekers• Reveal latent skill-sets that can match talents with job opportunities• Allow incarcerated young offenders the opportunity to develop an employment plan before release • Create an innovative framework for new dialogue between jobseekers and career advisors • Stimulate entrepreneurial aspirations by identifying talents and interests.• To achieve an accumulated 5 year ROI of 6,803%Only a radical new approach such as ours will foster a sea change in the attitude of how to support the young unemployed, especially with those who may not have succeeded academically at school, the so called ‘Hardest to Help’. Wider and more detailed evaluations create better careers advice, new choices and opportunities to explore new entrepreneurial/employed career paths. For the initial target group (Not in Education, Employment or Training) even further education could now be recognised a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'ADVANCED METHODS FOR EVOLUTIONARY SEQUENCE ANALYSIS' (AMESA) under Seventh Framework.

assistant: **Objectives**

Sequence alignment is widely used in molecular biology. Despite its age, the challenge is still not fully resolved: no method can suit all tasks, new approaches are needed for the evolving questions and even traditional methods can still be improved. Although many alignment tasks are related, some are based on incompatible principles and their need for distinct tools is not always understood. Evolutionary sequence alignment, the focus of this application, is a pre-requisite for all comparative analyses and needed e.g. in agricultural and medical research.The project aims at developing new methods for evolutionary and comparative sequence analysis using a novel approach of modelling data and considering evolutionary information. These methods target two current trends in sequence analysis, the increasing size of datasets and the specific properties of data produced on next-generation sequencing (NGS) platforms, from several different angles. First, I will develop novel ways to extend existing alignments and compute very large alignments; second, by modelling the properties of NGS data, I will expand the possible applications of NGS methods in evolutionary analysis. The new methods are meant to replace my earlier approaches for phylogenetic sequence alignment and become internationally recognised tools in evolutionary inference.The proposed work consists of five sub-projects, each describing a specific use case. Methods 1 and 2 focus on evolutionary sequence alignment and inference of evolutionary change; Method 3 extends these to modelling of sequence features and their inference from data using comparative methods; Method 4 focuses on high-throughput sequencing data with a special emphasis on non-model organisms and the use of evolutionary modelling to exploit phylogene