RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Control of amyloid formation via beta-hairpin molecular recognition features' to be submitted under Horizon 2020.

assistant: **Abstract**

The aggregation of proteins into amyloid fibrils is involved in various diseases which place a high burden on patients, families, caregivers, and healthcare systems, including Alzheimer’s disease, Parkinson’s disease and type 2 diabetes. While the therapeutic potential of the inhibition of amyloid formation and spreading has been recognized, there is a lack of effective strategies targeting the early steps of the aggregation reaction.In BETACONTROL, I want to establish a structure-guided approach to the control of amyloid formation and spreading. I will develop small molecule and polypeptide-based ligands that interfere with the initial phases of amyloid formation and thereby suppress any toxic oligomeric or fibrillar assemblies. The ligands will target beta-hairpin molecular recognition features, which I found to be readily accessible in disease-related amyloidogenic proteins. Targeting beta-hairpins enables retardation of protein aggregation by substoichiometric amounts of the ligand, affording inhibition of amyloid formation at low compound concentrations. As the strategy addresses the common propensity of amyloidogenic proteins to adopt beta-structure, it will be applicable to a wide range of proteins associated with various diseases.BETACONTROL will yield molecular-level insight into the mechanistic basis of amyloid formation and spreading. Furthermore, it will elucidate the significance of beta-hairpins as molecular recognition features in intrinsically disordered proteins (IDPs) and highlight the applicability of these features as targets for interference with protein-protein interactions of IDPs. Ultimately, BETACONTROL will provide a novel therapeutic approach to a range of devastating diseases.


**Keywords:** amyloid, intrinsically disordered proteins, protei
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Targeted disruption of the type I phospatidylinositol phosphate kinase gamma gene and the effect on focal adhesion formation in a mouse genetic background' (PIPKIGKO), addressing dissemination and exploitation.

assistant: **Impact**

Phosphatidylinositol-4, 5-bisphosphate (PI-4,5-P2) is a lipid molecule that contributes to the formation and maintenance of focal adhesions, by promoting the formation of an indirect physical association between integrin molecules and the actin cytoskeleton.A splice variant of Type I phosphatidylinositol phosphate kinase gamma (PIPKIg), a key enzyme in the synthesis of PI-4.5-P2, has recently been demonstrated to localize to focal adhesions and may contribute to local synthesis of PI-4.5-P2 at the sites of focal adhesions.The goal of this proposal is to generate a targeted gene disruption of this splice variant of PIPKIg in a mouse genetic model, with the specific aim of studying the putative requirement for localized PI-4.5-P2 production in the formation and maintenance of focal adhesions.The phenotype of mice harbouring a disruption of the PIPKIg gene will be analysed using standard techniques, and their morphology and histology will be compared to mouse models containing disruptions of various focal adhes ion components.Most of the models that will be used for comparison have already been generated and characterized by the host laboratory. A cell biological approach will also be used, to study the formation and architecture of focal adhesions.Fibroblasts harbouring the targeted disruption of PIPKIg will be generated and the formation and composition of focal adhesions will be studied using immuno fluorescence, live cell imaging techniques and biochemical techniques.In this manner the specific PI-4,5-P2-depen dent steps leading to focal adhesion formation will be elucidated. An understanding of how focal adhesions are formed and maintained will advance our understanding of the processes leading to cancer formation and metastasis, immune cell malfunction and imp aired
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Water technology for middle east region' under Fifth Framework.

assistant: **Concept**

The prime objective of this proposal is to explore the most effective way to increase availability of water for several Mediterranean countries which have an urgent needfor fresh drinking water at the lowest possible cost. In the summer of 1999 there is an exceptional drought in the region and as there are fequent droughts, the emergency situation may prolong into the year 2000 or return in subsequent years. This proposal plans a joint research initiative that specifically targets long-range sustainable development in Mediterranean countries which will eventually help manage the scarce water resources in each country. This wil be enhanced by research co-operation betwen the Mediterranean Partner Countries. The project focuses on both water recycling and efficiency, while assessing the environmental impact of the proposed solutions.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Fifth Framework proposal on 'Renilla luciferase-yellow fluorescence protein chimerae as indicators for real-time monitoring of camp and camp-induced phosphorylation in living cells based on bioluminescence resonance energy transfer (BRET)'.

assistant: **Innovation**

Intracellular monitoring offers the great advantage of imaging and studying signalling pathways in real time. Two genetically encoded indicators for camp and camp phosphorylation will be developed based on bioluminescence energy transfer (BRET). A) A number of chimeras will be prepared by fusion of a donor protein, Refill Lucifer's (Relic) and an acceptor protein, the Yellow Fluorescence Protein (EYFP) to either end of protein kinas A (PKA) catalytic and regulatory subunits. The camp will disrupt the energy transfer by dissociating the subunits. The chimera with the best BRET characteristics will be used for transfixion and in vivo camp monitoring. B) A second sensor will be composed by the Relic and EYFP joined by the kinas inducible domain (KID) which contains a phosphorylation site for PKA. We expect that the phosphorylation-dependent conformant ional change of KID will affect the BRET between Relic and EYFP. These BRET changes will be studied in vitro and in vivo and will permit an intracellular view of phosphorylation dynamics in real time. The proposed indicators may find applications in intracellular screening for PKA inhibitors and as leader molecules for the des gin of chimerical indicators containing phosphate acceptor sites for other kinesis. The above BRET sensors are expected to offer several advantages and wider cellular applications over the Fluorescence Energy transfer (FRET) sensors reported so far since they do not require excitation illumination.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Physical Activities of Older Adults: Longitudinal Interdisciplinary Analysis' (PAOLInA).

assistant: **Concept**

The PAOLInA-project focuses on physical activities of older citizens of European countries. It begins with the analysis of the phenomenon “physical activities”, and their change in the course of the late and latest years of life. The goal is to show what is covered by the term “physical activities” (PAs), and to differentiate PAs into domains according to their purpose (e.g. sports in leisure time, PA for self-care, PA as form of transportation). The Experienced Researcher (ER) hypothesizes that the overall involvement of older adults in PA does not necessarily decline as the individuals are getting older, but that some types of activities may even increase or replace others. If these interdependencies are neglected in the measurement older adults may appear as more sedentary than they actually are. The ER will also examine the factors that influence the change of the PAs. She will look at the interplay between individual characteristics and the attributes of people’s living environments. As a result, patterns of PA promoting factors will be carved out. The research will be based on secondary analysis of various longitudinal data sets, such as the German Socioeconomic Panel (SOEP) and the Survey of Health and Retirement in Europe (SHARE). Multilevel modelling will be applied in the statistical analyses. Thus, the PAOLInA-project is innovative in several respects: It will differentiate the PAs, which – according to the state of the existing research – has rarely been the case. It will reveal the long-term developments in older adults’ engagement and involvement in PAs. It will match individual characteristics with environmental and regional indicators. The ER will introduce her perspective of health psychology into an institute that does not have this specialization yet.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Empowering Business Ecosystems of Small Service Enterprises to Face the Economic Crisis' (EBEST), addressing dissemination and exploitation.

assistant: **Impact**

The eBEST project addresses small companies (typically with less than 50 employees) providing services to other businesses, often by subcontracting, in different industrial sectors. In general the intended service companies perform added-value activities in the intermediate phases of the value chain and/or in the repair and maintenance phases. The eBEST project strategic objective is to set-up, experiment and promote the adoption of new collaboration practices within each business ecosystem and across the ecosystem borders, taking advantage of a shared knowledge, an agreed corpus or rules and codes of practice, and a suite of ICT tools to support the intended ecosystem dynamics. The eBEST project operational objectives are: • Presenting worldwide the offers of the single service companies and clusters, and the integrated offer of the ecosystem as a whole, by exploiting sectoral and multilingual ontologies and taxonomies. • Adopting trust-building mechanisms, codes of practice, work assignment rules and performance evaluation criteria to guide the fair coopetition of the target companies. • Providing the target companies with easily accessible ICT applications and services to enable communication and collaboration within ecosystems and with the external world. • Experimenting and improving in real-life conditions the functional coverage and user acceptance of the proposed approach, methodology and ICT functions. • Deploying the project results to the largest audience so as to reach a critical mass of user companies in the shortest possible time after project completion. The economical situation calls for fast answers. For this reason the above challenging objectives should be reached in no more than two years. This is feasible since the RTD performers bring to the eBEST
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Droplet Photoelectron Imaging' in the context of a Horizon 2020 call.

assistant: **Objectives**

Angle-resolved photoelectron spectroscopy of aerosol droplets (“droplet photoelectron imaging”) is a novel approach to study fundamental aspects of the electron dynamics in liquids and across interfaces. Our recent proof-of-principle studies demonstrate that droplet photoelectron imaging not only complements, but also significantly extends the range of accessible information over established methods. Two aspects are unique to droplets: Firstly, the droplet size can be varied over a wide range from submicrons to microns. While large droplets provide overlap with liquid microjet and bulk studies, small droplets offer additional control by acting as efficient optical resonators. These optical cavity effects can be exploited to control where in the droplet the photoelectrons are generated; e.g. surface versus volume. Secondly, comprehensive information about photoelectron kinetic energy and angular distributions can be obtained fast and in a straightforward way by velocity map imaging.Building on our proof-of-principle studies, we propose to exploit the versatility of the droplet approach to address fundamental questions regarding electron dynamics in liquids and across interfaces: Can this new tool provide the missing data for low-energy electron scattering in water and other liquids and resolve the issue of the “universal curve”? How do slow electrons scatter across liquid-gas and buried liquid-liquid/solid interfaces and how does this depend on the composition and curvature of the interface? How is the ultrafast relaxation dynamics of electrons following above-band-gap excitation influenced by electron scattering and confinement effects? Low-energy electron scattering is a determining factor in radiation chemistry and biology and a central aspect of the solvated electron
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Toward AI Systems That Augment and Empower Humans by Understanding Us, our Society and the World Around Us' (2014-2020) under Horizon 2020.

assistant: **Objectives**

The Humane AI initiative will develop the scientific foundations and technological breakthroughs needed to shape the ongoing artificial intelligence (AI) revolution. The goal is to design and deploy AI systems that enhance human capabilities and empower both individuals and society as a whole to develop AI that extends rather than replaces human intelligence. This vision fits very well into the ambitions articulated by the EC in its Communication on AI but cannot be achieved by legislation or political directives alone. Instead it needs fundamentally new solutions to core research problems in AI and human-computer interaction (HCI), especially to help people understand actions recommended or performed by AI systems. Challenges include: learning complex world models; building effective and fully explainable machine learning systems; adapting AI systems to dynamic, open-ended real-world environments (in particular robots and autonomous systems in general); achieving in-depth understanding of humans and complex social contexts; and enabling self-reflection within AI systems. The focus is on human-centered AI, with a strong emphasis on ethics, values by design, and appropriate consideration of related legal and social issues. The HumanE AI project will mobilize a research landscape far beyond the direct project funding and create a unique innovation ecosystem that offers substantial return on investment. It will result in significant disruption across its socio-economic impact areas, including Industry 4.0, health & well-being, mobility, education, policy and finance. It will spearhead the efforts required to help Europe achieve a step-change in AI uptake across the economy. The consortium, with 35 partners from 17 countries, including four large industrial members, will de
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Mechanisms and determinants of amyloid assembly induced by lipid membranes' goes beyond current state-of-the-art under Sixth Framework.

assistant: **Innovation**

Formation of fibrillar protein aggregates (amyloid) is associated with serious diseases of global concern, e.g., Alzheimer's, prion diseases, type II diabetes. Despite the expanding research the mechanisms and determinants of amyloid formation in vivo are poorly understood. In the in vitro studies, rather severe conditions are often necessary to initiate the amyloid assembly (extreme pH, high temperature). In contrast, phospholipid membranes have been recently discovered to facilitate, in an apparently very generic manner, the initial steps of polymerisation due to orienting, charge neutralizing, and destabilizing effects of the membrane interface on the protein structure. This project is focused on the amyloidogenic protein polymerisation at charged phospho lipid membranes.The main objectives are:1) to determine correlations between the changes in protein conformation and the course of aggregation;2) to define the key factors initiating and controlling the kinetics of the fibre formation on the membrane su rface; and3) to obtain quantitative information on the structure of phospholipid-induced fibres.The studies will rely on well- defined model lipid systems (solid-supported bilayers, vesicles, giant liposomes) and the use of a combination of conformation- (FTIR, CD, fluorescence spectroscopies) and interaction-sensitive techniques (quantitative FRET microscopy, AFM). This will allow us to examine both changes in the protein internal structure and inter-molecular interactions (lipid binding, oligomerization, macroscopic aggregation) in the same model lipid systems under similar conditions. Proteins and peptides to be studied include fluorescently labelled lysozyme, cytochrome c, histone H1, and amyloid forming model peptides based on IAPP, temporins, etc. The proposed
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'The Neurocognition of Liveness' goes beyond current state-of-the-art under Horizon 2020.

assistant: **Innovation**

What makes live experiences special? Liveness is not just central to the performing arts, but to political rallies, sporting events, virtual reality and distance learning in higher education. NEUROLIVE is an inherently interdisciplinary research project, that combines theoretical concepts from theatre and performance studies, artistic research in performance making, psychological experimentation and mobile neuroimaging to understand how liveness is generated and experienced. NEUROLIVE proposes that the live experience can be conceptualized and quantified as a form of sustained entrainment, in which the minds, brains and bodies of performers and spectators are linked. Across three research streams we will combine artistic research (I) and cognitive neuroscience (II) to delineate the contextual, experiential and neurocognitive components of liveness, resulting in four live performance experiments (III). These live performances will be co-developed by performing artists and cognitive scientists with a view to establish an empirically testable, ecologically valid, multidimensional measure of liveness. NEUROLIVE combines dynamic experience sampling with psychophysiology, and mobile EEG. Advanced modelling techniques from machine learning will be used to integrate information from the different experiential, physiological and neural signals. This new measure will be grounded in the aesthetic principles of contemporary live performance, yet should be applicable across artistic and non-artistic performance situations, and to digital liveness in augmented and mixed realities. In this way, NEUROLIVE implements a conceptually and methodologically novel approach to understanding what makes live experiences special.