RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Adaptive and maladaptive endothelial cell dynamics during blood flow-driven vascular patterning' (ADAPT2FLOW).

assistant: **Concept**

The formation of a functional patterned (adaptation) vascular network is essential for development, tissue growth, and organ physiology. Several human vascular disorders arise from the mis-patterning (maladaptation) of blood vessels, such as arteriovenous malformations, aneurysms, and diabetic retinopathy. Blood flow is recognized as the main inducer for vascular patterning, yet very little is known about the molecular mechanisms that vascular patterning.C. Franco and others recently highlighted that endothelial cells polarize and migrate against the blood flow direction. Yet, how this behavior contributes to the overall process of vascular patterning is completely unknown. This project aims to study the dynamics of endothelial cells in vascular patterning during development, homeostasis, and disease. Taking advantage of a unique new transgenic mouse line reporting endothelial cell polarity in vivo, I will investigate endothelial cell behavior in vivo, including endothelial cell polarity, collective and individual migration and cell rearrangements, using 2-photon microscopy. I will extend this analysis to understand the involvement of endothelial flow-dependent polarity in formation and development of arteriovenous malformations. Finally, I will perform an in vivo functional screen to identify novel regulators of endothelial flow-dependent polarity, using a combination of automated image analysis and hemodynamics modelling.This integrative approach, based on high-resolution imaging and unique experimental models, will provide a unifying model defining the cellular and molecular principles involved in adaptive and maladaptive endothelial cell dynamics during blood flow-driven vascular patterning. Given the physiological relevance of vascular patterning in health and dise
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Freshwater integrated resource management with agents' demonstrating excellence, impact and implementation quality for a Fifth Framework call.

assistant: **Abstract**

Problems to be solvedThe aim of the FIRMA project is to improve water resource planning through the use of multi-agent models that integrate hydrological, social and economic aspects of water resource management through the representation of stakeholder decision making.Specific objectives are:· To further cooperation between water resource decision makers, experts in water resource management and experts in agent-based social simulation.· To analyse hydro social issues of waste water treatment, water scarcity and integrated catchments planning in selected regions.· To develop agent-based models for application to issues of water treatment, scarcity and planning.· To carry out participatory integrated assessments in five regions.· To develop a methodology for the modelling of water resource issues in Europe applicable to policy formulation.Scientific objectives and approachThe project is structured around three main themes:· Methodological development of agent models that scale from individual, cognitive decision makers to institutions representing more aggregate behaviour. Generic methodological knowledge will be encapsulated in a set of 'core' models that will be applied throughout the project.· The agent-based models will be demonstrated in five regions of Europe, linking the modelling and application teams in addressing real water problems.The case studies are: Thames water region, England Limburg, The Netherlands Barcelona, Spain Herault, France Zurich, Swizerland· Transfer of knowledge, experience and techniques, between experts on agent-based modelling and experts on water resource management and involving an interaction between the project and the wider community of policy makers.Expected impactsStakeholder participation is an essential feature of the project, st
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Durable resistance against fungal plant pathogens' (DURABLERESISTANCE) targeting Seventh Framework.

assistant: **Abstract**

Plants and their pathogens are in a constant process of co-evolution. Consequently, many of the known defense genes of plants against fungal pathogens are rapidly loosing effectiveness under agricultural conditions. However, there are examples for durable resistance. It is one of the main research questions in plant biology to determine the genetic basis of such naturally occurring resistance and to understand the underlying biochemical and molecular cause for durability. This durability is characterized by the apparent inability of the pathogen to adapt to the resistance mechanism. The molecular understanding of durable resistance will contribute to future attempts to develop such resistance by design. We want to use two approaches towards understanding and developing durable resistance: the first one is based on the naturally occurring durable resistance gene Lr34 against rust and mildew diseases in wheat. This gene was recently isolated in our group and it encodes a putative ABC type of transporter protein, providing a possible link between non-host and durable resistance. Its function in resistance will be studied by genetic and biochemical approaches in the crop plant wheat, as there is no Lr34-type of resistance characterized in any other plant. However, there is a close Lr34-homolog in rice and its function will be investigated in this diploid system. The second approach will be based on natural diversity found in a specific resistance gene, conferring strong, but not durable resistance. This diversity will be used for a designed improvement of durability by developing new proteins or protein combinations to which the pathogen can not adapt. We will use the 15 naturally occurring alleles of the Pm3 powdery mildew resistance genes to identify the structural basis
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'Clonidine for Sedation of Paediatric Patients in the  Intensive Care Unit'.

assistant: **Objectives**

The majority of critically ill children admitted to Paediatric Intensive Care Units (PICU) will require sedationand analgesia which is commonly achieved with a combination of an benzodiazepine and an opioid. However,these agents have a significant side-effect profile, including tolerance, withdrawal and respiratory/circulatorydepression. Clonidine is commonly used for sedation in PICU and recommended by guidelines in variouscountries although there is a lack of evidence regarding it safety and efficacy in this setting. The need for safetyand efficacy data as well as an age appropriate formulation for clonidine has been realised and clonidine isincluded in the EMA “Revised Priority List for Studies into Off-patent Medicinal Products”. Thus this proposaladdresses an important paediatric therapeutic need. It is designed to fullfill the requirements for most ethicalresearch in the paediatric population considering risk minimisation for patients, avoiding unnecessary studies andmake use of already available data as outlined in the Paediatric Regulation (EC) No 1901/2006. The objectivesof this project are a) to develop an age appropriate formulation of clonidine suitable for sedation of children inPICU b) to conduct a randomised, phase III, double-blind, active-controlled parallel group clinical trial of clonidinevs midazolam in patients from birth to 18 years to establish the efficacy and safety, including long-term outcomesand dose-dependent effects of clonidine and c) to establish an European consensus guideline for sedion ofcritically ill children. The ultimate goal is to use these data and to apply for a PUMA. On this basis a PaediatricInvestigation Plan (PIP) has been approved by the EMA in February 2013 and is reflected in the work plan ofCloSed. The project will incre
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon Europe proposal for the project 'Virtual reality emotion training tool'.

assistant: **Objectives**

The ERC starting grant project CoPAN (804582) is an innovative, interdisciplinary cross-boundary project. In the project, we study the function, underlying neurophysiological mechanisms, development and evolution of emotional expressions, which has shown us that emotions play a pivotal role in daily life in humans and great apes alike. We also show that emotional expressions in real life are more complex than the acted expressions used in emotion research or applications. I here propose to build on the fundamental knowledge and develop a virtual reality training tool targeting the broader public and clinical populations.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Biomechanical Characterization of  Carotid Plaque Rupture' (BIO-CAPTURE) targeting Horizon 2020.

assistant: **Abstract**

Stroke is the second leading cause of mortality worldwide. Most stroke events are triggered by an atherosclerotic plaque rupture in a carotid artery. Current clinical decisions for the carotid plaque removal surgery are made without assessing the plaque rupture risk as no reliable means exists today. Biomechanics can help to develop such risk assessment tools; yet, the fundamental knowledge on biomechanical descriptors of plaque rupture is missing.This project aims to provide biomechanical characteristics of atherosclerotic plaque rupture. This will be done by combining experimental work, high-end plaque imaging and computational modeling. First, non-existing biomechanical plaque rupture data will be collected from a novel experimental approach of physiological, ex-vivo inflation/rupture tests with atherosclerotic human carotid arteries. The pre-rupture and rupture strain distributions in plaques during the tests will be assessed in 3D via a cutting-edge high frequency ultrasound technique. With a novel inverse finite element (FE) technique, heterogeneous material properties of the plaques will be identified. This unique material information will be used in plaque-specific FE models to compute 3D pre-rupture and rupture stress distributions in the plaques. The assessed plaque strain and stress fingerprints will be evaluated for their predictive value of plaque rupture. The essential knowledge on biomechanical characteristics of plaque rupture will aid making more accurate surgical treatment decisions to prevent stroke-related mortality and morbidity. Besides the high-impact research, this fellowship will also result in enhancement of the applicant’s skill set through the valuable training on ultrasound imaging, histology and pathomorphology examination. Furthermore, the
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'A comprehensive analysis of essential protein regulators of clathrin-mediated endocytic pathway' goes beyond current state-of-the-art under Seventh Framework.

assistant: **Innovation**

Clathrin-mediated endocytosis is an essential molecular process involved in the maintenance of cell homeostasis of all eukaryotic organisms. It is indispensable for the uptake of nutrients and signaling molecules, for the proper composition of plasma membrane, and for the regulation of multiple signaling pathways. Since endocytosis is a primary entry point to the cell interior, its detailed knowledge is of key importance for many biomedical disciplines like immunology, neurophysiology and pharmacology. Studies on endocytosis at neuronal synapses or on its role during viral infection are just two examples of current research linked to potential therapeutic applications. Clathrin-mediated endocytosis is an elaborate, highly dynamic process assisted by more than 60 proteins, the majority of which is evolutionarily conserved. Different multiprotein modules are involved in the initial sorting of endocytic cargo, formation of an endocytic vesicle, and its subsequent scission and internalization. Although many components are known, molecular mechanism and spatiotemporal order of endocytic events are only being to be understood. Moreover, the key endocytic factors are essential, multifunctional proteins of modular structure, which greatly complicates their analysis in human. We plan to study the role of essential endocytic genes by combining quantitative real-time fluorescence microscopy with the powerful genetics of yeast Saccharomyces cerevisie. Crucial endocytic proteins will be depleted from the cell and endocytosis will be followed by live-cell imaging. Complementation assays with truncated/mutated constructs will be used to delineate the molecular details of missing function. Electron microscopy techniques will be introduced to reveal structures of obtained endocytic inte
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Boride-derived Highly Efficient Material for Green Hydrogen Generation and Zn–air Battery' (HYDROBAT) targeting Horizon Europe.

assistant: **Abstract**

The world energy demand is expected to grow by more than 25% by 2040, according to the world energy outlook by the International Energy Agency. To tackle this significant and urgent challenge new materials with engineered properties for energy applications are required. Hydrogen (H2) production by water electrolysis (Green H2) has received great interest as an alternative sustainable and clean energy technology. For instance, the green H2 is very crucial for the European Commission’s net zero emissions scenarios for 2050. To achieve green H2, this technology should be coupled with intermittent and renewable energy sources. And thus, the energy storage devices are involved in the green H2 production system. Due to its high energy density, and environmental-friendly, the Zinc-air battery is regarded as important energy storage device in the near future. But, the lack of satisfactory cheaper bifunctional electrocatalysts (ECs) for Hydrogen Evolution Reaction (HER) and oxygen evolution reaction (OER) for water electrolysis and the lack of bifunctional air ECs for oxygen reduction reaction (ORR) and OER are the main challenges for both these technologies. The state-of-the-art ECs are based on noble metals, whose application at a large scale is limited due to their high cost and scarcity. Consequently, the development of highly efficient ECs without precious metals is urgent. Current strategies for the development of desirable earth-abundant materials are often limited because of the poor electron transfer, low conductivity, poor stability, and small surface area of the active materials. To tackle this challenge, we aim (1) to develop hybrid materials based on earth-abundant metals derived from boride and highly conductive carbon by chemical reduction and pyrolysis methods, (
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Generating monoclonal antibodies that selectively and specifically recognize epitranscriptomic modifications.' (EPIMODKIT) under Horizon 2020.

assistant: **Objectives**

Epitranscriptomic modifications constitute specific marks in mRNA that regulate gene expression. Identification ofepitranscriptomic modifications requires next generation sequencing analyses and specific validation, processes that are cumbersome and time consuming. Furthermore, while some modifications are straightforward to identify, as they cause specific base changes (like RNAediting), most are only detectable either through chemical means (requiring large quantities of material) or by immunoprecipitationfollowed by sequencing. Of these techniques, immunoprecipitation requires far less material and can be used in any molecularbiology laboratory, but depends exquisitely on the quality of the monoclonal antibodies that recognize the modification - theirspecificity, selectivity and sensitivity.Most anti-modification antibodies are generated using traditional methods for the production of monoclonals to smallmolecule haptens (e.g. conjugation to a large protein carrier and injection into animals together with an adjuvant). While thesemethods can generate anti-hapten responses with some specificity they are generally not very selective. We have developed a novelimmunization platform that exquisitely focuses the antibody response to a hapten to generate high affinity monoclonals with a highdegree of specificity. We propose to employ this approach to generate anti-inosine antibodies (for the detection of editing in diseasecontexts); anti-m6A antibodies (which would be benchmarked against the ones currently in use, validating our approach); andantipseudo-uridine antibodies (a tool that is lacking in the field and for which traditional approaches have completely failed). The generation of specific and selective antibodies to these three modifications, will open the way toward
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'CACHE: CAlcium in a CHanging Environment' goes beyond current state-of-the-art under Seventh Framework.

assistant: **Innovation**

Anthropogenic driven climate change is a global problem that will increasingly affect our world and It is essential that we train our future scientists in multidisciplinary approaches to enable them to tackle such complex problems. This ITN examines environmental calcium mobilisation and deposition in marine molluscs, species that have been highlighted as being particularly at risk under future climate change scenarios due to the acidification and warming of the World’s oceans. However, surprisingly little is known about how these animals regulate calcium to produce a shell, how these processes might be affected when the environmental conditions change and what the consequences are at the population level. This lack of knowledge significantly impacts on our abilities to accurately predict future biodiversity and the consequences for the commercial aquaculture industry. We aim to remedy this knowledge deficit with this ITN. We will take an in-depth comparative approach, using four of the EU’s most important commercially exploited molluscan species as model organisms and examine natural variation in shell production in combination with experimental manipulations to quantify adaptive potential and identify novel genes/proteins that underpin responses to environmental change. By embedding our projects in natural population surveys, we will gain an unprecedented understanding of the level of phenotypic plasticity that operates in bivalve shell production: an essential prerequisite for understanding their resilience to environmental perturbation. The resulting data will also be integrated into models aimed at predicting future aquaculture scenarios and will lead into efforts at biomimic exploitation for sustainable building materials, providing a genuinely innovative inter-se