RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Comprehensive analysis of yeast expression-fitness functions (EFFs)', explaining its relevance to EU policy priorities under Horizon 2020.

assistant: **Impact**

Cellular and developmental processes rely on the precise activity of the proteins that constitute them, and alteration of protein activity often causes disease phenotypes and fitness defects. The relation between protein activity (or expression) and organismal fitness is thus a fundamental genetic property with implications for evolutionary and disease processes. However, a systematic understanding of how protein expression relates to fitness is lacking.Here we propose a comprehensive screen of expression-fitness functions (EFFs) for yeast genes.The proposed inter-disciplinary project will combine cutting-edge methods from synthetic biology, innovative one-step construction of a comprehensive genome-wide library, multiplexed genomics measurements and functional genomics computational analyses to elucidate fundamental patterns of expression-fitness functions.The methodology is based on an inducible degron system that allows for controlled repression of protein levels. Due to a one-fits-all design, the inducible degron can be fused in a one-step transformation procedure to any gene contained in the yeast GFP-fusion library. An all-in-one construction of the library, assessment of library components via deep-sequencing and multiplexed growth and expression assays will allow for the measurement of thousands of yeast gene EFFs in parallel.Computational data analyses will elucidate principal classes of EFFs. Subsequent functional genomics analyses will link principal EFFs to underlying molecular and physiological properties, such as gene’s functions, pathways or cellular localizations, which will therefore provide a foundation to understand the principal causes of how alterations in gene activity translate to impairments of cellular function.The proposed project will therefor
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon 2020 proposal on 'A multi-sited and transnational study of transitions towards post-fossil fuel societies'.

assistant: **Concept**

Worlds of Lithium is a multi-sited and transnational study that examines how the strategic replacement of fossil fuels with electric transport powered by lithium-ion batteries is taking place in Chile, the largest lithium producer worldwide, China, the world leader in lithium-ion battery production, and Norway, likely to become the world’s first ‘zero emission’ electric vehicle country. The overall research questions of the project are: How do planetary strategies to decarbonize transport act upon the landscapes and societies where a) lithium is extracted, b) turned into technology and c) used and potentially reused and recycled? How can the different ways in which these strategies take place inform non-destructive approaches to collaborative responsible actions to phase out fossil fuels—or decrease their utilization?Worlds of Lithium will innovatively answer these questions through a transnational documentation of the knowledges and practices through which scientists, grassroots communities, policy makers, and lithium-ion battery producers are differently responding to ecological transformations and disruptions at stake in transitions to sustainable societies. The project will explore such knowledges and practices with a special focus on the effects strategies to respond to global warming have on existing socio-economic and eco-political planetary inequalities. By studying how planetary efforts for decarbonizing transport trigger different processes in resource-making, the project will not only enrich the anthropology of natural resources, but it will also create ground-breaking conceptual tools to support, think, and document emerging collaborations for sustainability capable of powering planetary eco-responsibility. Thus, the project will innovatively theorize new im
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Determination of the changes in modification status of SUMO-1 and SUMO-2 sub-proteomes' (DCMS), addressing dissemination and exploitation.

assistant: **Impact**

The ubiquitin-like proteins SUMO-1 and SUMO-2 are covalently conjugated to a wide range of target proteins where they serve as subunits of complex protein associations (sub-proteomes). These covalent modifications play important roles in cellular processes such as transport, regulation of transcription, signalling, and others. In this proposed project I would like to take advantage of several advanced techniques in molecular cell biology and mass spectrometry.In the first part of my study I will define the proteins covalently interacting with each of the SUMO paralogues in human cells. To achieve this I will use cell lines with integrated versions of TAP-tagged SUMO-1 and -2, which will allow me to recover them together by affinity with other tightly bound proteins. I will then determine how the abundance of proteins modified by SUMO changes during the course of the cell cycle and in response to stress conditions such as UV, heat and hypoxia.Quantitative changes in modified proteins will be determined by mass spectrometry using SILAC (Stable Isotope Labelling by Amino acids in Cell culture) technique. These experiments will add significant, new and valuable data concerning SUMO sub-proteomes. Obtained results should allow for creation of complex and hopefully quite clear picture of the relationships among all the analysed proteins.Summarizing, these data should help our understanding of the many cellular processes not necessarily directly involved in the SUMO sub-proteomes. Lists of SUMO targets will be made available to the scientific community so that groups with an interest in a particular protein can determine the role of SUMO modification in its function.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Newtinst ss' (1998-2002).

assistant: **Innovation**

The Isaac Newton Institute for Mathematical Sciences is an international institute that covers the broad spectrum of mathematical sciences. During 2000 the Institute will hold a number of inter-disciplinary programmes and one Euro Summer School will take place during several of these. The objective is to ensure that the work of the Institute is disseminated more widely in the European Community and that younger people from that community are given the opportunity to participate in a wide range of mathematically related sciences. The programmes are linked by the fact that they all explore an important topic at the cutting edge of the mathematical sciences but it is expected that different scientists will attend each and hence a large number of young people could be involved in the series as a whole.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Investigating new therapeutic approaches to Friedreich's Ataxia' (FAST) targeting Seventh Framework.

assistant: **Abstract**

Friedreich’s Ataxia (FRDA) is a devastating degenerative disease with no specific therapy. It is passed by autosomal recessive inheritance and affects 1:30,000 individuals in Caucasian populations. Symptoms appear in the first decade of life and include progressive and unremitting lack of movement coordination, leading to complete inability, and dilated cardiomyopathy leading to congestive heart failure, the most common cause of premature death. FRDA is due to the insufficient transcription of the gene coding for the mitochondrial protein frataxin. Reduced cellular levels of frataxin cause impaired mitochondrial function and increased sensitivity to oxidative stress, leading to accelerated cell death in critical tissues.Severity of the disease critically depends on residual frataxin levels. Therapeutic efforts are mostly focused on increasing cellular frataxin . We found that frataxin is normally degraded by the ubiquitin-proteasome system. We identified the lysine responsible for the ubiquitination of frataxin and, by computational screening followed by experimental validation, we identified and validated a series of small molecules, called ubiquitin-competing molecules (UCM), that prevent frataxin ubiquitination and induce frataxin accumulation in cells derived from FRDA patients. Moreover, treatment with UCM partially rescues aconitase and ATP production defects in cells derived from FRDA patients.Our goal is two fold: 1) submit a set of leads we already identified, as well as their new and more complex derivatives, to preclinical testing in FRDA mice  2) identify the E3 ligase that is responsible for frataxin ubiquitination, and investigate the possibility to use it as a druggable target for small molecules to prevent frataxin degradation.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Unlock the full power of your Point of Sales' demonstrating excellence, impact and implementation quality for a Horizon 2020 call.

assistant: **Abstract**

The Luntarium new web and mobile application simplify work for sales reps, stockers and merchandisers selling consumer goods into retail stores and supermarkets while giving full visibility to managers at head office. The software is an innovative and disruptive web platform and mobile technology solution targeted to mass consumer products companies in the EU. The application has high powerful tools in a simple user interface that allow companies monitor prices, out of stock, product availability, product range control, geotagged photos of store fronts, shelves or promotional materials and more for brands and its competition in real time. It has integrated collaboration features like messages, announcements and client notes are a great tool for team management while all reps activities are Geo tagged and monitor. With the tool teams can perform surveys, audits and order management activities. The prototype software was built with Wisis Rapid Ajax Application Framework developed by our key partner Infologic S.A., leading edge technology for extreme fast customized software development at the highest level of client expectations using the latest Java / J2EE and Ajax technology environment. The need of this unique software was discover while working closely with Group Danone, multinational market leader of daily and mass market products. Danone needed to boost sales at the point of sales, and responds to its competitors and leverage their customers’ with new ways of shopping by mobilizing their customer experience, it’s partner’s stores, and it’s entire value chain. The client could not find any software available on the market. The software prototype has been already implemented successfully in 2014 at Danone Argentina with excelent feedback from 50 fields’reps. We need t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Protein S-mycothiolation and real-time redox imaging in Corynebacterium diphtheriae during ROS stress and infection conditions' in the context of a Seventh Framework call.

assistant: **Objectives**

Glutathione serves as the major thiol-redox buffer in the defense against Reactive Oxygen Species (ROS) in eukaryotes. Firmicutes bacteria utilize as thiol redox buffer bacillithiol (Cys-GlcN-Mal, BSH) and Actinomycetes produce the related redox buffer mycothiol (AcCys-GlcN-Ins, MSH). In eukaryotes, proteins are post-translational modified to S-glutathionylated proteins in response to oxidative stress. S-glutathionylation has emerged as major redox-regulatory mechanism and protects cysteine residues against overoxidation to sulfonic acids. Using thiol-redox proteomics and mass spectrometry (MS) we have recently discovered protein S-bacillithiolations as mixed BSH protein disulfides in response to oxidative stress in Firmicutes bacteria. Protein S-bacillithiolation controls the activity of the redox-sensing OhrR repressor and protects active site cysteine residues of metabolic enzymes, antioxidant function proteins and translation factors. However, it is unknown if ROS and infection conditions cause protein S-mycothiolations and affect the cellular MSH redox potential in pathogenic Mycobacteria and Corynebacteria. Here we aim to explore the comprehensive mycothiolome in the major respiratory pathogen Corynebacterium diphtheriae.We apply gel-based and novel MS-based thiol-redox proteomic approaches for the quantitative analysis of the S-mycothiolome in C. diphtheriae under oxidative stress conditions (e.g. NEM-Biotin-Switch-Assay). Novel genetically encoded redox biosensors (Mrx1-roGFP2 and roGFP2-Orp1) will be developed for real-time imaging of the MSH redox potential and ROS production during infections in C. diphtheriae. The role of S-mycothiolated proteins for redox regulation, fitness, stress resistance and virulence mechanisms will be investigated. Our studies provi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'Joining worlds apart: Observations and Simulations of the First Stars in the Universe'.

assistant: **Objectives**

The First Stars in the universe are of great importance in the astrophysical context as a probe of the early universe, although the existence of their survivors has not yet been established in searches of metal-poor stars. The simulations of the First Stars have a key role in not only identifying them but also gaining insight into the evolution of the universe and the star formation history of our Galaxy. The applicant's specialized knowledge in simulating the First Stars in the global 1D stellar evolution approximation will help advanced stellar simulation including non-standard physics at the host institute. Several aspects of these first stars are different from stars born more recently, like our sun. Differences are especially important in the mixing physics (complex convective events) that evade the 1D approach. The applicant's models will provide the understanding of the overall astrophysical context especially for multi-dimensional effects such as convection, rotation and magnetic fields on the mixing episodes carried out by the Keele group. The proposed research project will lay the foundation for future long-term collaboration as we are just beginning to explore the complex mixing physics in the First Stars. The applicant has also conceived and implemented a database of the metal-poor stars, and developed tools that allow effective interpretation of new model results. The increasing number of these important tracers of the early Universe allows a statistical foundation of general properties of the First Stars. The applicant's expertise, database and tools are unique and not yet public. These knowledge components will greatly enhance the ability of the Keele group in this area to systematically apply their advanced, hydrodynamics based models to observations and
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Goodness of fit and predictive ability in survival analysis' under Fourth Framework.

assistant: **Concept**

Research objectives and content Survival analysis is a specific branch of statistics whose aim is modelling the time to an event. Examples of 'failure' time include the lifetime of machine components in industrial reliability, the duration of strikes or period of unemployment in economics, the survival time of patients in clinical trials. In any of the mentioned situations model checking is a crucial step of the analysis. The first objective of this project is developing suitable procedures for identification of anomalous observations (individuals who are poorly predicted by the final model). Further investigation of these subjects may be of great impact in medical research. As second goal we wish to develop measures of model predictive capability. though prediction is often the final task both in medicine and economics, this topic has received relative little attention in survival analysis. Thus a contribution in this direction can stimulate research in an open field.  Training content (objectives, benefit and expected impact) A deeper knowledge in survival analysis can be acquired by collaborating with the research team of the host institution.  Links with industry / industrial relevance (22)
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Unaided Sharp and Continuous Vision in All Daily Activities' (SYMBIOSE).

assistant: **Concept**

When getting older, everyone suffers from a loss of visual continuity, either from presbyopia or opacification of the eye’s lens (called cataract). What’s more, cataract surgery likewise leads to presbyopia. Despite continual innovation in the domain of ophthalmological implants called IOLs (intraocular lens), cataract patients do not recuperate sharp and continuous vision.Today, choosing an IOL is a question of constraints and compromises for both the surgeon and the patient.These problems can be relegated to the past thanks to the SYMBIOSE IOLs portfolio. SYMBIOSE IOLs are ophthalmic implants integrating a continuous phase optical profile, patented by Cristalens Industrie. SYMBIOSE IOLs allow continuous, quality vision in all daily activities.In order to validate this product and go to market, Cristalens Industrie faces a twofold challenge:- clinical, to collect enough data demonstrating the complete system’s high performance and thus convince Key Opinion Leaders worldwide,- industrial, to deploy the innovation in a product portfolio and ensure the scale-up of production, intellectual property, regulatory and marketing issues.These challenges define Cristalens Industrie's work program which includes four work packages in addition to management:1/ Demonstrate the performance of the complete system in 300 patient cases,2/ Prepare to scale up to ensure manufacturing capacity meets customer demand,3/ Convince the scientific and medical community,4/ Remove regulatory barriers and secure IP aspects.Cristalens Industrie aims to establish itself as the innovative leader for the next generation of IOLs.


**Keywords:** Surgery, Intraocular lens, Cataract, Presbyopia, Clinical medicine, Ageing, Clinical studies, Scale-up
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Highly efficient and sustainable refrigeration based on solid state Magnetic Cooling Device' demonstrating excellence, impact and implementation quality for a Horizon Europe call.

assistant: **Abstract**

Cooling and refrigeration account for 7.8% of global greenhouse gas emissions and 20% of electricity demand. The standard refrigerant F-gases have a tremendous global warming potential (GWP) and are being phased out in the EU. Available alternative gases are either toxic or explosive, posing serious issues for their applicability.Magnotherm has developed the first industrially feasible refrigeration unit based on magnetic cooling technology that uses a specific class of material and alternating magnetic fields. This is achieved thanks to a patented process to mass produce highly performant, stable and cheap magnetocaloric materials using abundant, noncritical materials; and to interdisciplinary backgrounds and world-leading research resulting from TU Darmstadt.Magnetic cooling implements a solid state cooling cycle using no refrigeration gas, with 0 associated GWP, no risk to the environment and no issues of flammability. It is x3 more efficient and achieves 40% electricity savings.


**Keywords:** magnetic cooling,magnetocaloric materials,alternating magnetic fields,solid state refrigeration cycle,gas free refrigerator,domestic refrigerator, food and beverage refrigerating display cabinet