RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Sixth Framework proposal for the project 'Membrane transporters: In vitro models for the study of their role in drug fate'.

assistant: **Objectives**

The goals of MEMTRANS project are to optimize and pre-validate in vitro cultured cell models to predict oral absorption and pharmacokinetics of efflux systems substrates (P-gp, MRP2, BCRX for instance), to establish an in vitro cut off for the risk of secretion associated problems and to study the structure-affinity-transport relationships. That would allow:1) A reduction in the number of animal experiments to study efflux processes;2) The in vitro study of drug-drug (including phytopharmaca), drug-food, interactions related with secretion transporters;3) To obtain useful information for modelling transporter interactions in other normal and transformed tissues (blood brain barrier, lung mucosa, tumours).The objectives of the project will be achieved by using a systems biology approach that involves modelling and simulating the complex dynamic interactions between proteins (transporters), metabolites (i.e substrates) and cells (lipoidal barriers). Computational and mathematical predictive models will be generated from the data based on the system parameters and in the drug characteristics. The experimental data generated in the project will be analysed from a mechanistical point of view in order to split all the individual steps involved in transport. The mathematical models in combination with the right physiological, physicochemical and chemical information could be applied to predict drug transport in a wide range of membranes in the organism (blood brain barrier, liver, kidney, tumours etc.) but especially in the gastrointestinal tract.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Ageing well: european study of adult well being (ESAW)' goes beyond current state-of-the-art under Fifth Framework.

assistant: **Innovation**

Ageing Well: A European Study of Adult Well-Being (ESAW) would produce a European Socio-Cultural Model for Ageing Well, which estimates the direct causal contribution of five key components (physical health and functional status, mental efficacy, life activity, material security and social support) with personal characteristics and culture to the outcome variable ageing well. The project would be conducted in six countries: Austria, Italy, Luxembourg, The Netherlands, Sweden and the United Kingdom. The research would yield an integrated dataset from six national samples of 2000-2400 adults aged between 50and 90. Partners from the multidisciplinary consortium would produce reports on each research theme and the European Socio-Cultural Model for Ageing Well. The findings will inform domestic and international policy development, and guide the delivery of professional care and services to older people across Europe.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Seventh Framework proposal on 'Theoretical multiphoton spectroscopy for understanding surfaces and interfaces'.

assistant: **Concept**

The project will develop new methods for calculating nonlinear spectroscopic properties, both in the electronic as well as in the vibrational domain. The methods will be used to study molecular interactions at interfaces, allowing for a direct comparison of experimental observations with theoretical calculations. In order to explore different ways of modeling surface and interface interactions, we will develop three different ab initio methods for calculating these nonlinear molecular properties: 1) Multiscale methods, in which the interface region is partitioned into three different layers. The part involving interface-absorbed molecules will be described by quantum-chemical methods, the closest surrounding part of the system where specific interactions are important will be described by classical, polarizable force fields, and the long-range electrostatic interactions will be described by a polarizable continuum. 2) Periodic-boundary conditions: We will extend a response theory framework recently  developed in our group to describe periodic systems using Gaussian basis sets. This will be achieved by deriving the necessary formulas, and interface our response framework to existing periodic-boundary codes. 3) Time-domain methods: Starting from the equation of motion for the reduced single-electron density matrix, we will propagate the electron density and the classical nuclei in time in order to model time-resolved vibrational spectroscopies.The novelty of the project is in its focus on nonlinear molecular properties, both electronic and vibrational, and the development of computational models for surfaces and interfaces that may help rationalize experimental observations of interface phenomena and molecular adsorption at interfaces. In the application of the methods de
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Massive 2 to 3 two-loop amplitudes in asymptotic limits for collider phenomenology' to be submitted under Horizon Europe.

assistant: **Abstract**

The data taken at the Large Hadron Collider (LHC) at CERN and its future High-Luminosity upgrade enables the study of the fundamental interactions of matter at unprecedented precision and energy.  After the discovery of the Higgs boson one of the major goals of the LHC is the precise measurement of its properties. In order to achieve the needed theoretical precision, two-loop corrections to many scattering amplitudes have to be computed.A crucial process to precisely determine the coupling of the Higgs boson to the bottom quark is the production of a Higgs, a Z-boson, and a jet. This process receives large contributions and accompanying uncertainties from the sub-process of gluon fusion, which only starts contributing at the one-loop level but is enhanced by the large gluon luminosity at hadron colliders. To improve the theoretical description of this sub-process and enable the most precisedetermination of the Higgs-bottom coupling, the two-loop five-point amplitudes of this sub-process will be computed in this project. The results of this project can also be applied to double Higgs production, which the crucial experimental signature to determine the Higgs self-interaction at hadron colliders in the future.The computation of five-point amplitudes with massive internal and external particles is a tremendously difficult task, due to the algebraic complexity of the amplitudes and the appearance of new classes of special functions. To tackle and circumvent some of the difficulties I will explore the systematic approximation of these complicated amplitudes in various kinematic limits, like small Mandelstam variables or the high-energy limit. This methodology has already proven successful for two-loop four-point amplitudes previously. The results of this project will, theref
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Fragility and sustainability in restricted island environments: adaptation, cultural change and collapse in prehistory' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

Sustainability of societies in a restricted or fragile environment forms a perpetual question, past and present underpinning questions of the rise and fall of civilisation.  Today, the eroded Maltese islands support one of the densest human populations in the world. When first colonised in the 6th millennium BC, pristine soil and forest covered the landscape, but within centuries the landscape was bare and under intensive cultivation. The Neolithic perpetrators of the original clearance developed a sophisticated if isolated island civilisation in the face of the precarious environment and limited natural resources. Their megalithic temples focused on ritual feasting whilst complex social organisation controlled food distribution. Over centuries, Malta maintained an intensive subsistence economy and a controlled population, but 2500 years after the first settlement that socio-economic system seems to have imploded resulting in the collapse of its island civilisation.What precise conditions led to this collapse, and was there complete or only partial economic and cultural failure? Did external factors - episodes of climatic instability, sea-level change, disease, invasion – impact irreversibly on the island inhabitants, or did over-intensification cause internal political and economic failure? Were the islands abandoned or was life sustained on them? Were other small islands affected in similar ways? These questions underpin my investigation which aims to extract eroding and fragile environmental and archaeological evidence from rapidly deteriorating landscapes.  The goal is to understand the relationship between environmental change, stress and cultural continuity or collapse. I will address these questions through application of modern interdisciplinary environmental sc
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Reconfigurable silicon architecture for connected intelligent lighting.' (SmartComm-LED).

assistant: **Concept**

Lighting accounts for 19% of energy consumption in the developed world. However low energy lighting technologies are rapidly replacing the traditional incandescent technology and by far the most rapidly growing low energy lighting technology today is LED. The LED lighting market was worth $16.5B in 2013, is growing at a CAGR in excess of 16% and is projected to continue growing at this rate through to 2018 when the market is projected to be worth $33.5B.Not only is lighting moving towards more energy efficient technologies such as LED, but the way in which lighting is used is also undergoing a dramatic evolution. Lighting is becoming increasingly “connected” and increasingly “intelligent” and it is this growing market that Ikon Semiconductor aims to address with this proposal.The target customers for the outcome of this project are manufacturers of LED lighting power supply systems. The project aims to develop an optimum re-configurable silicon architecture for “connected intelligent lighting” systems for use in smart buildings including residential, commercial and industrial. It will enable a single highly configurable chip-set solution with an optimum hardware/software co-design to operate with any wireless or wired communications protocol and will allow the developers of advanced lighting power supply systems to re-configure their design to address a broad range of applications. This will replace the currently available disparate range of fixed-function solutions and will enable reduced cost and time-to-market for our target customers. Such a solution has global commercial potential.In this Phase-1 SME instrument feasibility study we aim to use both new and existing end-user advisor relationships to carry out a market validation and an economic viability analysis. It
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon 2020 proposal on 'Type VI-dependent Pseudomonas aeruginosa phospholipases and host manipulation'.

assistant: **Concept**

IntroductionPseudomonas aeruginosa (Pa), a nosocomial pathogen, secretes a wide range of virulence factors. A recently described secretion pathway is the type VI secretion system (T6SS). Pa encodes three T6SSs, H1-, H2- and H3-T6SS. The best-characterized H1-T6SS is involved in delivering toxins into bacterial competitors.State-of-the artTwo phospholipases (Pld), PldA and PldB secreted via H2- and H3-T6SS, respectively, were recently identified as trans-kingdom virulence effectors, triggering both killing of bacterial competitors and internalization into non-phagocytic cells. They are encoded within two distinct pld clusters that are not present in all Pa isolates.ObjectivesOur study should (i) decipher the prevalence of the two pld clusters among Pa isolates, (ii) elucidate the role of those Pld during in vivo infection, (iii) confirm the Pld contribution in the T6SS mediated entry, (iv) dissect the PI3K/Akt signalling pathway activation mediated by Pld during Pa entry.Overview of the actionThe aim is to link the fundamental study of new virulence factors with their relevance into clinical isolates.MethodsPa strains isolated from infection or environment will be screened for the expression of pld clusters. The role of PldA and PldB as virulence factor will be assessed using a murine model of respiratory tract infection. In vitro invasion assays will be performed to validate the role of PldA in the H2-T6SS-dependent entry in non-phagocytic cells. Upstream and downstream signalling events of Akt phosphorylation induced by PldA and PldB upon infection will be dissected by performing cell infections with mutants and specific inhibitors.Originality and innovative aspects of the projectBacteria-host interaction mechanisms are instrumental in the development of infectious dis
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Structural and Functional Characterization of the RZZ Complex' goes beyond current state-of-the-art under Seventh Framework.

assistant: **Innovation**

This application is concerned with the structural and functional characterization of the RZZ protein complex, a critical constituent of metazoan kinetochores. The RZZ, a 400-kDa trimer, is named after the initials of its components, Rod, Zwilch and Zw10. The RZZ is implicated in the execution of mitosis, the process of equal partitioning of the genetic material. In mitosis, the sister chromatids attach to spindle microtubules and become aligned at the metaphase plate. This alignment process is orchestrated by the dynamic interaction of microtubules with kinetochores, the site of end-on attachment for microtubules to mitotic chromosomes. By recruiting the minus-end directed microtubule motor dynein, the RZZ complex contributes to form initial, lateral attachments of kinetochores to microtubules. The RZZ is also required for kinetochore recruitment of Mad1 and Mad2, two essential constituents of the spindle assembly checkpoint (SAC). Through biochemical reconstitution and structural methods, we will gain an understanding of the yet unknown organization of the RZZ. We will also develop biochemical assays to probe the interaction of the RZZ with defined kinetochore and SAC components, thus gaining an insight into the role of the RZZ in the larger framework of kinetochore organization and dynamics.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'OPTIMISATION, OPERATIONAL ENVIRONMENT TESTING AND APPROVAL FOR USE OF NOZZLE RRZ IN HIGHWAY TUNNELS AS AN ACTIVE FIREFIGHTING SYSTEM' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

According to the European Commission, 70,000 people are hospitalized due to severe injuries caused by fire with an estimated damage of €126.3 billion every year. Highway tunnels are currently protected by passive fire systems such as fire-resistant construction materials that slow down the spread of a fire. However, this can be insufficient during major fires hence need for active fire protection systems. The analysis of fire incidents has indicated that fires in tunnels tend to be hotter with temperatures over 1,000oC, last longer even up to 53 hours making rescue operations and fire extinguishment more difficult. The Fire Protection System Market is growing at a fast pace, expected to grow from €44.36 billion in 2016 to €79.44 billion in 2022, at a CAGR of 9.7%. PNR is an Italian company company with 50 years’ experience in the fire protection industry. PNR has developed Nozzle RRZ, a tunnel fire fighting nozzle that can produce a high capacity jet at 130 litres per minute (lpm) with a 6 meters spray coverage operating at normal installation level. Nozzle RRZ will reduce energy consumption in fire fighting in tunnels by ≈1620%, from 1755MJ to 102MJ; reduce operating pressure by ≈3100%, from current 60 -160bars to 2-5 bars; improve water economy and fire delay due to wide drop size range of 50 – 500microns and larger spray area cover. Through this PH1 we seek to validate the technical, commercial and financial feasibility of Nozzle RRZ. Post validation of the project feasibility we will move on to the PH2 through which we seek to finalise the technical developments of Nozzle RRZ, conduct demonstration and validations tests to prompt industry adoption and initiate commercial adoption by players in highway tunnels and rail tunnels. Making use of a PH2 budget of €1.5m and
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'IMPROVING AGRI-ENVIRONMENTAL POLICIES: A SIMULATION APPROACH TO THE ROLE OF THE COGNITIVE PROPERTIES OF INSTITUTION AND FARMERS' (IMAGES).

assistant: **Concept**

Objectives of the projectThe project will provide new directions for improving the definition of agri-environmental policies in the European Union.The study will compare the evolution of agri-environmental policies and actions of six local areas in France, Italy and the UK. It will model the beliefs and knowledge of farmers, the social interactions between the farmers and other agents, and the agri-environmental situation in each locality. A simulation will be created from these models which will be valuable to policy makers al the regional, national and EU levels in improving agri-environmental politics.Zones of studyThe study will focus on 2 zones in each EU state involved in the project:- In France, 2 zones will be chosen among Ari0ge, Jura, LozOre, Auvergne, Var.- In Ihe United Kingdom, Breadblane Environmentally Sensitive Area (ESA) of Scotland, and the North Yorkshire ESA will be selected,- In Italy, lands in the Po valley and Friuli Hills (North East Italy) will be selected.OrganizationThe project involves 4 partners specialized in the socio-economy of and agriculture and environment (I team of Cemagref, University of Edinburgh, University of Milan, University of Udine), and 3 teams specialized in cognitive and social simulalions (1 team in Cemagref, University of Surrey, Ecole Normale supérieure).Main steps of the project1. The data collection and treatment will be performed in parallel to the specifications and implementation of the simulation model. 2. The software will be tested, analysed on collected data, and submitted to the criticism of a panel of farmers and institutional actors. The models will be improved after initial tests and lacking data will be collected. 3. A second analysis of the software will be performed in an explorative purpose.4. Dissemina
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Role of reactive microglia and intraneuronal amyloids on neuronal vulnerability in early Alzheimer’s disease' to be submitted under Horizon Europe.

assistant: **Abstract**

The recently approved drugs for Alzheimers disease (AD), targeting amyloid plaques removal, only mildly slow patients cognitive decline as neuronal loss starts decades before plaque deposition. To delay AD onset before symptoms development, prevention of neuronal death should be prioritised instead. An early AD pathological event is intraneuronal accumulation of amyloids (intraA) which can cause neuronal death at the pre-plaque stage. IntraA is only found in specific neuronal subtypes first to be lost in AD, suggesting it might underly their selective vulnerability. Reactive microglia  could contribute to intraA as blocking neuroinflammatory processes in early AD reduces its accumulation. Whether microglia also contribute to intraA+ neurons selective loss is still unknown but could suggest repurposing of already available microglia targeting drugs for early neuroprotective therapies. I will test this hypothesis in 5xFAD mice, first confirming intraA accumulates in the neuronal subtypes selectively lost, as it occurs in humans. I will analyse the contribution of reactive microglia to intraA neurons death  by pharmaceutically rescuing their reactivity and determine if intraA neurons-associated microglia express AD high-risk genes, which drive late AD pathology. Because AD affects women more, possibly due to their higher inflammatory response, the sex-specificity in the previous analyses will also be tested. Finally, I will establish microglia-containing human brain organoids to recapitulate this synergy in a in vitro human AD model, providing a foundation for future drug testing.By determining the potentially detrimental interplay between microglia reactivity and intraA+ neurons, using both in vivo and in vitro models, my project will lay the basis of extensive future wor