RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'When neurons run hot: γ-Secretase thermal vulnerability as an Alzheimer's pathogenic mechanism' (ThermAPP).

assistant: **Concept**

Alzheimer’s disease (AD) is the leading cause of dementia, yet current therapies provide only modest benefit. Mutations in presenilin (PSEN1/2), the catalytic subunit of γ-secretase (GSEC), cause familial AD by destabilizing the GSEC–APP/C99 complex and shifting processing toward longer, aggregation-prone Aβ42/43 peptides. Importantly, even wild-type GSEC produces small amounts of these species, suggesting that subtle complex destabilization also contributes to sporadic AD. This raise a critical unresolved question: what triggers this instability in the absence of mutations?I hypothesize that neuronal thermogenesis destabilizes GSEC–APP complexes, promoting pathogenic Aβ production. Synaptic mitochondria, acting as local bioenergetic hubs, can generate transient temperature rises of several degrees. Our preliminary data show that fever-range heating induces “FAD-like” Aβ profiles, supporting temperature as a direct pathogenic driver.Aim 1 will define thermal thresholds for GSEC dysfunction by quantifying Aβ peptide ratios across controlled temperature gradients in iPSC-derived neurons and synaptosomes.Aim 2 will establish the causal link between mitochondrial heat production and pathogenic Aβ using genetically encoded thermosensors in live neurons.Aim 3 will assess thermal susceptibility of GSEC in neurons transdifferentiated from aged and AD patient fibroblasts, integrating thermogenesis, aging, and APP processing.This project introduces thermodynamics as a novel mechanism in AD pathogenesis and paves the way for translational opportunities. Identifying temperature-sensitive molecular nodes may enable development of small-molecule GSEC stabilizers or mitochondrial modulators as disease-modifying therapies for both familial and sporadic AD.


**Keywords:** Gamma-secreta
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Fourth Framework proposal for the project 'Low cost, high wear resistance sintered powder components'.

assistant: **Objectives**

The objective of this proposed project is to develop and demonstrate a process for the production of near net shaped components with very high resistance to wear. The technology to be developed is to use a selfpropagating high temperature synthesis (SHS) route to produce carbide containing metallic particles (termed X-TiC) which can be added to conventional iron and low alloy powders which can then be processed by a powder metallurgy(PM) operation. The development of this process will open up new applications and markets for PM parts, reduce wastage of worn components and raw materials and increase international competitiveness for European countries, particularly those involved in PM processing and machinery and equipment manufacture employing high performance components.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'High-Entropy MXene Inks for Light-Induced Sintering of Flexible Zn-Air Batteries' to be submitted under Horizon Europe.

assistant: **Abstract**

The FlexZAB proposal introduces an innovative approach to producing a new generation of printed flexible Zn-air batteries (ZABs), addressing key challenges such as sluggish oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) kinetics and high overpotential in traditional ZABs, while preserving the integrity of the support structure without traditional high-temperature sintering during manufacturing. The central component of this approach in FlexZAB is the synthesis and use of high-entropy MXenes (HEMXs), which play a pivotal role in both the fabrication process and the improved performance of the ZAB.MXenes, a family of two-dimensional (2D) carbides and nitrides of transition metals, are highly regarded for their flexibility, superior electrical conductivity, and partially metallic properties. Their 2D morphology makes them ideal for flexible applications, and they exhibit plasmonic effects under light irradiation, akin to Au and Ag. Incorporating multiple transition metals at the M site in HEMXs introduces a nearly infinite range of tunable compositions and properties. This leads to a quasi-continuous density of electronic surface states, promoting complex and reversible catalytic reactions such as ORR and OER.During flexible ZAB fabrication process of FlexZAB, the plasmonic properties of HEMXs will facilitate light-triggered sintering during manufacturing. The flexibility and interconnect of HEMXs serve as binders, enabling the production of additive-free inks and cathodes through aerosol direct-write printing. In battery operation, HEMXs boost performance by enhancing charge transport through high electrical conductivity. Additionally, their compositional versatility and abundant surface adsorption/reaction sites catalytically accelerate ORR and OER,
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Trained immunity: improving the next generation of vaccines for the older generation' demonstrating excellence, impact and implementation quality for a Horizon 2020 call.

assistant: **Abstract**

The host defense, especially the adaptive immunity, is defective in the elderly, with a dramatic drop for the efficacy of vaccination with old age. Interestingly however, the innate immunity of older individuals is relatively intact, and we recently described that epigenetic and functional reprogramming of innate immune cells by certain vaccines and mild infections, termed ‘trained immunity’, induces potent heterologous protection against infections. I propose that induction of trained immunity is an important novel approach to improved vaccination in the elderly. Induction of trained immunity is regulated by the interaction between the host genome, microbiome, and the epigenetic and metabolic programs of specific populations of myeloid cells, and we need to understand how these factors are impacted by age and gender of the host. By understanding the factors that impact the response to BCG (Bacille Calmette-Guerin), the prototype vaccine that induces trained immunity, we will be able to design better vaccines for the elderly.  The Key objectives of the project are:  Key objective 1: To describe the innate immune cell (sub)populations, and their heterogeneity at single-cell level, responsible for mediating trained immunity in the young and elderly adults. Key objective 2: To identify the complex genetic, epigenetic, microbiome, and metabolic programs that represent the molecular and biochemical substrates of trained immunity in the myeloid cells of the elderly individuals. Key objective 3: To use systems biology to map the heterogeneity of trained immunity response determined by host (epi)genome, microbiome, and environmental factors in the elderly.  Expected results: We will understand the main cellular and molecular mechanisms for the induction of trained immunity resp
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon Europe proposal for the project 'Feeding the Roman Army, Making the Empire'.

assistant: **Objectives**

FRAME capitalises on advances in archaeological science and a critical mass of archaeological material to examine how the Roman Empire’s frontiers in Europe functioned as economic as well as militarised zones. The Empire’s expansion and longevity relied on the capability to maintain large armies and provincial garrisons for long periods. The army comprised c. 300,000 soldiers, mainly concentrated on frontiers and supply was a vast challenge, yet how it was achieved remains poorly understood. International in scale, this multi-disciplinary project will explain how the army was supplied with food and the impact this had on the landscapes and economies of frontier provinces and beyond. The Roman Empire’s frontiers are iconic, and their extensive remains have been a focus of research for centuries. Traditionally viewed as physical and cultural boundaries between civilised Romans and their ‘barbarian’ neighbours, Rome’s frontiers in Europe are ready for this ground-breaking project that will reappraise their functions and roles and make a major contribution to the study of Roman imperialism’s remarkable resilience and longevity.FRAME focuses on the somewhat neglected (in Roman studies) resource of food remains (animals, plants and organic residues in ceramics) in five frontier regions. By combining cutting-edge multi-isotope and organic residue analysis with archaeological and historical evidence, the project will reconstruct the military diet across frontiers, address how food was produced, the networks that supplied it and the impact this had on frontier landscapes and economies. The ambitious analytical programme will reveal the regional strategies core to the functioning of the Roman Empire and how they were affected by cultural choices and environmental constraints. Thi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Control and complexity in quantum statistical mechanics' (Cocoquest).

assistant: **Concept**

Developing a comprehensive theory of thermodynamics at the quantum scale requires a fundamental change of perspective with regards to its classical counterpart. On the one hand, measuring a quantum system will induce a non-negligible back-action and an energy cost that is at least comparable to the energy scale of the system. On the other hand, controlling microscopic degrees of freedom and harnessing quantum effects provides novel opportunities with no classical counterpart. In light of these facts, control over different quantum features and quantum measurements themselves become valuable resources that need to be integrated into any self-contained theory of quantum thermodynamics. In this project, we aim to address this grand challenge. First we will develop a formal theory that includes control over quantum systems with fixed complexity to incorporate these concepts into thermodynamic considerations at the quantum scale. Second, we will unveil the fundamental thermodynamic limitations on information acquisition by constructing a thermodynamically self-contained description of the quantum measurement process. Finally, we will carefully study current quantum technologies and, with the help of our own lab and experimental colleagues, we will devise experimental proposals and perform proof-of-principle experiments based on existing quantum technologies, to make sure that the theoretical considerations describe relevant features.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Seventh Framework proposal on 'Space-based analysis of the relationship between vegetation functioning and atmospheric CO2 and CH4 greenhouse gases'.

assistant: **Concept**

Vegetation physiological activity and atmospheric CO2 and CH4 greenhouse gases are key factors in the regulation of the carbon cycle. Atmospheric CO2 is linked to vegetation functioning by a bidirectional process in which vegetation modulates the seasonal amplitude of CO2 concentration through photosynthesis and respiration processes, while CO2 affects vegetation growth by the so-called fertilization process. In addition, the role of plants as a non-negligible CH4 source is currently under active discussion. The research presented in this proposal is aimed to the analysis of the correlation between green vegetation functioning and atmospheric CO2 and CH4 concentrations at regional and global scales by the exploitation of satellite data. Data acquired by the Greenhouse gases Observing SATellite (GOSAT) launched in January 2009 by the Japanese Space Agency will be used to derive space and time-resolved atmospheric and vegetation variables for this multidisciplinary analysis. The quantitative and global assessment of (a) the link between vegetation functioning and atmospheric CO2 concentration and (b) CH4 emission by plants are the two main goals of this proposal. The researcher would benefit from the broad experience in the field of the host Atmospheric, Oceanic and Planetary Physics department of the University of Oxford for the achievement of the proposed research objectives. The topic of the space-based assessment of the carbon cycle is considered of major importance by European research directives. In particular, this topic perfectly fits into the Environment (including climate change) and Space themes of the Cooperation specific program of the EU-FP7, and into the Coordination Action COCOS (COordination action Carbon Observation System).
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'PTX3 in Humoral Innate Immunity' goes beyond current state-of-the-art under Horizon 2020.

assistant: **Innovation**

The innate immune system includes a cellular and a humoral arm.  Structural diversity is a characteristic of humoral fluid phase pattern recognition molecules. These include  complement components, collectins, ficolins, and  pentraxins. We have used the long pentraxin PTX3, identified by the applicant (cDNA and genomic, mouse and  human), as a prototypic fluid phase pattern recognition molecule to dissect its function, as well as to define general properties of humoral innate immunity and its interplay with the cellular arm. The general objective of this application is to explore unexpected vistas on humoral innate immunity, using PTX3 as a molecular tool.  Specifically two hypothesis will be tested  based on preliminary data.  First the applicant will test the hypothesis that matrix and microbe recognition are related functions of PTX3 and that a microenvironmental signal (acidic pH) sets PTX3 in a matrix recognition, tissue repair mode. A second related line of work will focus on inflammation as a key component of the tumor microenvironment. The applicant  will test the hypothesis that PTX3  and elements of the humoral  innate immune system are essential components of cancer related inflammation.  In particular, based on preliminary data,  the hypothesis  will be tested that PTX3 acts as an extrinsic oncosuppressor in murine carcinogenesis and in selected human cancers by suppressing the recruitment of tumor-promoting inflammatory cells. These studies are expected to provide new unexpected vistas on the humoral arm of the innate immune system.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Small RNA-directed control of the plant and animal innate immune responses' (2007-2013) under Seventh Framework.

assistant: **Objectives**

The innate immune response is the first line of defence against pathogens, which is initiated by the detection of pathogen-derived signatures referred to as Pathogen-Associated Molecular Patterns (PAMPs). Plants and animals sense PAMPs and in turn differentially regulate a large set of immune response genes, among which microRNAs (miRNAs) were recently identified. In the model plant Arabidopsis thaliana, dozens of miRNAs are PAMP-responsive, among them we found that a conserved miRNA contributes to antibacterial resistance. More recently, we have reported a major role of the Arabidopsis miRNA pathway in antibacterial defence and, as a corollary, have identified a series of bacterial-derived suppressors of the miRNA pathway. This pioneering work represents an important contribution to the understanding of virulence strategies employed by pathogenic bacteria and suggests that analogous strategies may also be used by human pathogenic bacteria.In the proposed project, we will first aim to investigate the extent to which an RNA silencing suppression strategy employed by a phytopathogenic bacterial effector is also used by effectors from human pathogenic bacteria. We will additionally aim to generate a comprehensive view of small RNA repertoires produced in the course of bacterial infection using both a human and a plant pathogenic bacteria. The second aspect of our proposal is directed toward a better understanding of the influence of PAMPs and bacterial effectors on Arabidopsis transcriptional gene silencing (TGS), a pathway that silences transposable elements and repeats through the establishment and maintenance of cytosine DNA methylation. Finally, we will aim to identify and characterize bacterial effectors that interfere with TGS. Overall, these studies should reveal co
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Replacement of sulphur dioxide (SO2) in food keeping the SAme qualitY and shelf-life of the products' under Seventh Framework.

assistant: **Concept**

Up to today there are many foodstuffs (including beverages and wine) where sulphites are used as antioxidant and preservative agents. Examples for this foodstuff are dried fruits, some kinds of fruit juices, seafood and convenience food (especially containing potatoes). But these sulphites containing agents are in suspicion to cause some damage to the health and they were blamed for some allergy.   Aim of the project is not to find a universal replacer for sulphites containing agents for all foodstuffs, but to develop tailor made solutions to the following foodstuffs  - dried fruits  - wine and beverages - convenience food (especially containing potatoes) - package and processing.  For each line of products special expert groups will be established. These expert groups chose 3 to 5 products for detailed research. While these groups are working on additives the group “package and processing” will focus on solutions within the process. This could be the environment were the process takes places but also solutions within the package are possible. The process of research is closely linked with a legislation- and sensory evaluation part. This should guarantee clean products referring to the marketability and their sensory properties.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Photopolymer rapid opto-electronic manufacture of macro/micro prototype products (PROMPT)' to be submitted under Fourth Framework.

assistant: **Abstract**

Objectives and content:The final objective of this proposal is the straight away manufacture of parts and moulds made of ceramic or metal by stereolithography (SL), by adding fillers (ceramic or metallic powders) to the curable resin. To reduce the present fabrication times and costs, our really innovative approach consists in manufacturing a part layer by layer and no longer point by point. SL allows manufacturing complex three-dimensional parts and enabling many thousands of companies (automotive, aerospace, medical design engineers, etc.) to turn their CAD drawings into full-scale models rapidly, speeding up their new product designs. Its present principle is the following: the part is manufactured layer by layer by curing a liquid photosensitive resin with an UV laser beam, which is deflected by two computer-controlled mirrors. So every layer, and consequently the part, is manufactured point by point.This leads to a trade-off between processing speed and part resolution, precise parts requiring long processing times. Our project consists in manufacturing parts, no longer point by point, but layer by layer directly by shaping the laser beam: the light itself will carry the design of the layer prior to irradiate the open surface of the resin. It will then be possible to manufacture a layer by one UV irradiation only: its processing time will become independent of the complexity of its design. Different ways to shape the beam will be conceived, UV lasers having to be used for chemical reasons. One of the partners of this consortium built a feasibility set-up of these SL machines of the next generation, proving the viability of the project. For a rapid demonstration with a liquid crystal display as dynamic masks generator, he used a visible laser. The obtained accuracy