RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Transannular Macrocyclisation and the concise total synthesis of Antifungal natural products', explaining its relevance to EU policy priorities under Sixth Framework.

assistant: **Impact**

The Barrett group has carried out extensive research on the synthesis of antifungal natural products and the underlying synthetic methodology with recent focus on targets such as nikkomycin B, papuamine, papulacandin, FR-900848, pramanicin, the palmarumyci ns and preussomerin G.This proposal concerns the total synthesis of two bioactive natural products zearalenone and 15G256b. These two macrocyclic compounds are respectively a mycotoxin and an antifungal agent. They possess a highly functionalised macrocycl ic core and 2,4-dihydroxybenzoic acid units, which are constituents of numerous macrocyclic bioactive natural products.The first objective of the research is the development of new and original strategies, which should allow concise access to these natural compounds. The first key step is the macrocyclisation reaction, which takes place by using an unusual metathesis reaction between an alkene and an alkyne, following an approach recently developed at Imperial College London. The second key step involves a transannular reaction to elaborate the arene entity fused to the macrocyclic ring by a biomimetic closure of triketo-ester moiety. The second objective of this work is the biological testing of the synthesised compounds by GlaxoSmithKline in the United Kingdom to evaluate their activities.This proposal describes a programme of interdisciplinary research. First, it is a synthetic chemistry because these polyfunctionalised compounds will be prepared by using original strategies and new methodologies. Secondly, it is also a medicinal chemistry because this route to zearalenone and 15G256b is also promising in that it could be applicable to others members of these classes of natural products and also allowing for the determination of structure-activity relationship wi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Silicon/Carbon Nano-Hybrid Lithium-Ion Battery Anode: Green Facile Scalable Synthesis Inspired by Thermosetting Polymers' in the context of a Horizon 2020 call.

assistant: **Objectives**

The last two decades have witnessed a great commercial success of lithium ion battery (LIB) in portable electronic devices and electric vehicles. However, current LIB technology cannot meet the rapid increasing demand from information technology and vehicle industry, primarily due to limited capacity and serious safety concern of graphite anode. Discovering new anode material with high capacity and good reliability has been a central issue. Because of its high theoretical capacity and excellent operation safety, silicon (Si) has attracted considerable attention as a promising anode to replace graphite. Nevertheless, dramatic volumetric change during lithiation/delithiation process causes severe pulverization and disconnection of electrode from current collector, leading to a fast capacity loss. To tackle these critical problems, a new concept to achieve facile, cost effective, green, and scalable synthesis of Si/carbon nanohybrid anode is proposed. By reducing Si particle size to the range below 10 nm and homogeneous embedding of Si nanoparticles into carbon buffer matrix, the volume change and associated stress can be effectively accommodated to improve the cyclability of the LIBs. Instead of conventional aqueous and/or organic solvents, three industrially widely used thermosetting resin monomer systems including vinyl ester resin, epoxy resin, and phenolic-formaldehyde resin are utilized as both solvent and carbon source. Cost effective silane coupling agents bearing appropriate chemical functional groups act as the precursor of Si and cross-linking agents of the thermosetting polymers. Ultra small Si nanoparticles are in situ formed and homogeneously embedded in the in situ formed porous carbon matrix by sequential photo/thermally induced polymerization, calcination
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Climate Sensitivity of Glacial Landscape Dynamics' under Horizon 2020.

assistant: **Concept**

How do erosion rates in glacial landscapes vary with climate change and how do such changes affect the dynamics of mountain glaciers? Providing quantitative constraints towards this question is the main objective of COLD. These constraints are so important because mountain glaciers are sensitive to climate change and their deposits provide a unique history of Earths terrestrial climate that allows reconstructing leads and lags in the climate system.The climate sensitivity of mountain glaciers is influenced by debris on their surface that impedes ice melting. Theoretical models of frost-related bedrock fracturing predict that rates of debris production are temperature-sensitive and that its supply to mountain glaciers increases during warming periods. Thus a previously unrecognized negative feedback emerges that lowers ice melt rates and potentially buffers part of the ice retreat due to warming. However, the temperature-sensitivity of debris production in glacial landscapes is poorly understood. Specifically, we lack robust erosion rate estimates for these landscapes, which are key for testing models of frost-related bedrock fracturing.Here, I propose an innovative combination of new tools that capitalize on recent developments in cosmogenic nuclide geochemistry, landscape evolution modelling, and planetary-scale remote sensing analysis. I will use these tools to quantify headwall erosion rates in mountainous glacial landscapes and to gauge the sensitivity of mountain glaciers to variations in debris supply. Expected results will provide a basis for assessing the impacts of global warming, for improved predictions of valley glacier evolution, and for palaeoclimate interpretations of glacial landforms. COLD will focus on glacial landscapes, but the inverse modelling appr
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Biomimetics for Functions and Responses' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

Energy efficiency and sustainability encourage to develop lightweight materials with excellent mechanical properties, combining also additional functionalities and responses. Therein nature allows inspiration, as e.g. pearl of nacre and silk show extraordinary mechanical properties due to their aligned self-assemblies. However, biological complexity poses great challenges and in biomimetics selected features are mimicked using simpler concepts. Previously artificial nacre has been mimicked by multilayer and sequential techniques and ice-templating. However, concepts for aligned spontaneous self-assemblies are called for scalability. We will develop toughened nacre-inspired materials by templating functionalized polymers on colloidal sheets in suspension, followed by self-assembly by solvent removal. Similarly, we will develop silk-mimetic materials using aligned organic fibrous reinforcements in soft dissipative matrix. Nanofibrillated cellulose will be wet-spun using extrusion into coagulant bath, followed by post drawing, drying and functionalization to allow silk-like fibers with high mechanical properties. In another route, cellulose rod-like whiskers will be decorated with soft functional polymers allowing energy dissipation, followed by alignment and interlinking to mimick silk-assemblies. The colloidal routes allow also new functionalities by using functional polymers, e.g. electroactive and conjugated polymers and nanoparticles. Importantly, redox-active polymers are bound on the colloidal sheets. Incorporating in a planar electrochemical cell with flexible electrodes, electrochemical switching of stiffness is obtained using a small voltage, as the intercolloidal interaction is controlled by the charge state of the redox-active layers. This would allow a new cla
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Aligned Natural Fibres and Textiles for Use in Structural Composite Applications' in the context of a Seventh Framework call.

assistant: **Objectives**

Biocomposites manufactured from natural materials such as fibres and bioderived polymers offer a sustainable alternative to traditional ones, but at present they are not available for use in structural applications. NATEX will develop aligned textiles from natural fibres suitable for use as high strength reinforcing fabrics to produce structural composite parts using bio and oil based thermoplastic and thermoset resins. This will include the use of orientated woven natural fibres in bioderived thermoplastics and thermosets, to produce high-tech products with high added value from entirely renewable resources. The main innovations will be: • New chemical/enzymatic treatments to tailor the fibre surface chemistry and to modify its cell wall polymers, to obtain the desired interface properties when combined with the polymer matrix •New chemical/natural treatments for the yarn during the wrapping process, new method for low twist yarns production, film stacking and commingling development for natural fibres, to increase the mechanical properties of the yarns •Development of new weaving techniques to improve impregnation and to obtain innovative 3D textiles •Resin viscosity control using thermal conductive additives, increasing their compatibility with natural fibres by using coupling agents and surfactant additives Besides, a large range of resin processing methods will be adapted to suit them to the characteristics of the modified fibres: Vacuum Bagging, Vacuum Consolidation, Compression Moulding, Continuous Compression Moulding, Infusion and Resin Transfer Moulding. Basic research on joining technologies as hot welding over natural fibre composites will be also performed. As result, aligned natural fibres with improved properties will be combined with thermoplastics and t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon Europe proposal for the project 'Reconstructing the Molecular Evolution of Non-Shivering Thermogenesis'.

assistant: **Objectives**

Non-shivering thermogenesis (NST) is one of the most fascinating evolutionary achievements, enabling high body temperatures and successful radiation of mammals and birds into cold environments. Yet, the evolutionary origin and molecular innovations enabling NST are unknown. Using comparative approaches, my laboratory has recently transformed our view on mammalian NST by demonstrating that the thermogenic function of the mammalian heater protein, uncoupling protein 1 (UCP1), has only emerged late during mammalian evolution in the stem placental ancestor.  With this ground-breaking framework, I aim to reconstruct the molecular innovations of UCP1-based NST in placental mammals, and explore the unknown molecular basis of NST in non-placental mammals and birds, thereby advancing our understanding on the evolution of thermogenesis in vertebrates. Specifically, I will 1) Determine critical structure-function motifs of UCP1 that enabled heat production and that have been tuned through placental species radiation to adapt thermoregulatory demands of various ecological niches; 2) Characterize molecular networks governing NST on an evolutionary scale by comparative transcriptomics of extant placental and marsupial heater organs; 3) Explore alternative thermogenic mechanisms in marsupials; 4) Delineate the functional evolution of muscle-based NST in amniotes by reconstructing ancestral key variants of sarcolipin, the proclaimed key player of muscle thermogenesis. In addition to cutting-edge molecular and bioenergetic techniques for assessing thermogenesis, which we have all mastered in my laboratory, our reconstruction of ancestral thermogenic proteins allows us to look back hundreds of millions of years into the evolutionary past of vertebrate thermogenesis. To identify unknown t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Seventh Framework proposal on 'ATP dependent nucleosome remodelling - Single molecule studies and super-resolution microscopy'.

assistant: **Innovation**

In eukaryotic cells the DNA is packaged into nucleosomes and higher order structures which lead to a condensation and protection of the DNA. During important cellular processes such as transcription or replication access to the DNA has to be granted. This is facilitated by ATP dependent nucleosome remodelling. The mechanistic details how the involved remodelling complexes succeed in providing access to the nucleosomal DNA are currently in spite of large experimental efforts not well understood. The aim of the proposal is to unravel the molecular mechanism of nucleosome remodelling using single-molecule fluorescence resonance energy transfer (FRET). By putting labels on the nucleosomal DNA, the histones or the remodellers we will - step by step - determine the conformational changes that occur during remodelling and use this information to build a mechanistic model. We will also use the controlled assembly of 30 nm fibers from purified components in order to determine how remodelling occurs in this structurally restricted environment. The large size of the chromatin fibers will dictate that in addition to FRET measurements, which will again be used to investigate local motion, super-resolution microscopy need to be employed to obtain information about long-distance movements. To this end we will use stochastic optical reconstruction microscopy (STORM), which allows for accuracy below 10 nm, thus ideally complementing the FRET approach. In summary our experiments will lead us to a mechanistic understanding of ATP dependent nucleosome remodelling, both on mono-nucleosomes as well as in higher order structures. This knowledge will in return stimulate new initiatives aimed at understanding the nature and regulation of chromatin dynamics in vivo.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon Europe proposal on 'RegulatoryTEmbryo: Dissecting the role of regulatory transposable elements in shaping embryonic gene networks'.

assistant: **Concept**

The precise regulation of gene expression is essential for embryogenesis. Transposable elements (TEs), often considered genomic parasites, are hypothesised to function as regulatory hubs coordinating gene networks during this critical window, but evidence remains largely correlational. The RegulatoryTEmbryo project will bridge this gap by dissecting the causal impact of regulatory TEs on Arabidopsis thaliana development.This project moves beyond correlation to establish causation through an integrated experimental strategy. First, I will use a dedicated bioinformatic pipeline and in vivo reporter assays to identify and validate the regulatory function of specific TE families. Second, I will employ multiplexed CRISPR-Cas9 to perform targeted deletions of these families, allowing for a definitive assessment of their impact on embryonic transcription and phenotype. Finally, I will use biochemical assays (EMSA) to mechanistically confirm the underlying transcription factor-TE interactions, and evolutionary approaches to uncover the general principles governing TE-encoded gene regulation in plant embryo patterning.This research will deliver a new model for the role of TEs as key drivers of developmental gene regulation. Ultimately, it will provide a mechanistic framework for understanding how TE-encoded gene regulatory activity fuels morphological diversity in nature. By integrating advanced cell biology and biochemistry with my expertise in epigenomics, this fellowship will provide the crucial interdisciplinary skills required to launch my career as an independent research leader.


**Keywords:** Transposable elements, developmental gene regulatory networks, embryogenesis, transcription factors, gene regulation, evolution.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'A European research training site for the design and synthesis of novel Neuroprotective and Hypoglycaemic Agents through a multi-disciplinary approach' under Sixth Framework.

assistant: **Concept**

EURODESY focuses on establishing a European Research Training site for the development of new agents for potential therapeutic intervention in neurodegenerative diseases and type 2 diabetes mellitus.The project aims to provide research training in the design and synthesis of novel antioxidants, neurosteroids, and cannabinoids, as neuroprotective agents, and thiosemicarbazones, 1,2,4-oxadiazoles of b-D-glucose derivatives and indole-imidazole hybrids, as potential hypoglycaemic drugs.This will be achieved by integrating multi-disciplinary fields encompassing synthetic organic and pharmaceutical chemistry, X-ray crystallography, biophysics, biological assessment and computational chemistry, in a joint effort under an efficient management structure.EURODESY objectives will be supported by the researchers at the National Hellenic Research Foundation (NHRF) in an attempt to develop a new generation of multi-skilled, and highly qualified scientists. Trainees will be equipped with multidisciplinary outlook and skills in order to shape the future of European research excellence in academia and industry.They will be trained in synthetic methodologies of organic, pharmaceutical and organometallic chemistry and in X-ray crystallographic, biophysical, biological and computational methods with the aim of facilitating the design and synthesis of new chemical entities as potential drugs. This training will be reinforced by familiarisation with industrial procedures for the manufacture of pharmaceuticals.The integration o f this multidisciplinary expertise within the NHRF will significantly enhance its existing scientific output in pharmaceutical development and health and strengthen its competitive position as a European centre of excellence in research areas of major socio-economic i
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'JAM-A and HER2/3 Antagonistic Strategy In Targeting breast cancer' under Horizon Europe.

assistant: **Concept**

Breast cancer is the most common female malignancy, and a leading cause of death. Although molecular technologies have led to new therapeutic targets for breast cancer, much remains to be done to reduce its societal and economic impact. This project focuses on an emerging cancer drug target – Junctional Adhesion Molecule-A (JAM-A), an adhesion protein that Dr Hopkins (Supervisor; RCSI) and others have implicated in breast cancer. This proposal centres around recent data suggesting that JAM-A controls the expression of HER2 and HER3, key tyrosine kinases associated with aggressive breast cancer. I will test the novel hypothesis that combining a JAM-A antagonist and an anti-HER2/HER3 drug (Pertuzumab) exerts synergistic anti-tumour effects in breast cancer settings. This will be accomplished by: Testing the combination of a JAM-A antagonist and an anti-HER2/HER3 antibody for anti-tumor efficacy in 2D and 3D models of breast cancer in vitro; Using chick embryo xenograft assays (cell line-based and patient-derived xenograft-based) to test the combination of a JAM-A antagonist and an anti-HER2/HER3 antibody in vivo; Identifying new JAM-A antagonists and test them for synergism with an anti-HER2/HER3 antibody in breast cancer settings;Validating a possible JAM-A-HER2/HER3 signalling axis in breast cancer patient datasets and samples. Combining an innovative, multi-faceted approach, and offering an academic multidisciplinary environment which will both complement my industrial skills and enable me to transfer valuable industry-centred knowledge, this project presents a wealth of opportunities to enhance by career prospects and enable a transition back to academia. As there are currently no anti-JAM-A drugs on the market or in clinical trials, this is a timely and important pro