RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Marine Environmental DynamIcs and seX-based analysis for climate change adaptation in marine spatial planning' in the context of a Horizon 2020 call.

assistant: **Objectives**

To effectively adapt to climate change (CC), a novel more dynamic approach in governing the oceans is needed. Marine Spatial Planning (MSP) – a public process aimed to allocate human uses at sea while maintaining multiple ecosystem services (ES) – as currently implemented is too static to adequately incorporate climate refugia and CC responses of marine life. The goal of my project is to provide a stronger, more complete model for the sustainable planning of marine ES in the California Current. MEDIX will significantly improve the traditional MSP process in a context of a fast-changing climate by creating a novel approach to MSP that incorporates a dynamic ES assessment under CC scenarios with a sex-based analysis of CC effects on species and ecosystems, to elaborate a guideline for adaptation actions in MSP. The California Current will provide an ideal case study to test the new approach for adaptation strategies to maintain the ES.Through the MSCA Global Fellowship, I will reinforce my expertise in ecosystem-based management in MSP with new interdisciplinary expertise and skills in ES modeling under CC and gendered innovations, which I will transfer back to Europe, where the development of adaptive strategies for MSP is relevant and timely. The GF envisages: i) an outgoing period at Stanford University, USA, where I will be involved in a major initiative to revise and adapt the marine protected areas network of the California Current within the national MSP initiative, in collaboration with governments, and ii) a return period at CNR, Italy, where I intend to build on and transfer the skills acquired to elaborate the adaptive MSP guidelines that I will make accessible to relevant European stakeholders through a process of science-to-policy and science-for-society enga
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'The role of JNK/c-Jun pathway in axonal damage and neuronal regeneration' (JNKNEUROREG) targeting Seventh Framework.

assistant: **Abstract**

Interruption of axons results in a characteristic reaction of the injured neuron, the so-called axonal response, which aims to induce axon elongation and to achieve a re-construction of effective synapses. Nerve fiber transection elicits a complex neuronal response that leaves neurons poised between death and regeneration. Although the signals underlying this dichotomy are not fully understood, some are mediated by the induction of immediate-early transcription factors of the AP-1 family, a key regulator in crucial cellular events. AP-1 activity is regulated, in part, by the phosphorylation of its c-Jun component by the Jun N-terminal Kinases (JNKs). Recent work has confirmed the notion that c-Jun functions as the trigger of the axonal response during neuronal regeneration in the CNS and strongly supports the perception of c-Jun action as a “double-edged sword” promoting post-traumatic neuronal cell death as well as axonal regeneration. The goal of this project is to better understand the function of the JNK/c-Jun pathway during neuronal regeneration by generating and employing gain and loss-of-function transgenic mice models and to clarify the role of JNKs/c-Jun phosphorylation in the neuronal response to axonal damage. The assessment of the reinnervation after injury in these transgenic lines will use suitable regeneration paradigms. We are also interested in identifying new JNK/c-Jun mediators that could play a role in the axonal response to injury. Thus, future perspectives towards the strengthening of European potential research could derive from this study. This project integrates well in the FP7 program, and will applicate knowledge and biotechnology for health. The accomplishment of the project would contribute significantly to the acquisition of new relevant da
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon 2020 proposal on 'Assessing the Response of Marine Macro- and Micronutrient Bioavailability and Cycling to Arctic Sea-Ice Loss'.

assistant: **Concept**

Sea-ice loss, freshening and stratification changes in the recent past have affected, and are projected to continue to affect, the timing, distribution and biomass production of Arctic primary producers. Current enhancements observed in primary production are attributed to increased light penetration associated with sea-ice thinning and loss. However, these enhancements may be a relatively short-term phenomenon that will recede unless there is additional supply of nutrients to the photic zone. In addition to external inputs through rivers, atmospheric deposition and vertical mixing, internal cycling and redistribution related to sea-ice formation, transport and melting are important mechanisms affecting nutrient distributions in the Arctic Ocean. Quantifying the individual contributions of these processes to nutrient bioavailability and cycling is imperative in light of ongoing sea-ice retreat but currently hindered by the lack of studies applying source- and process-sensitive tracers. Therefore, I propose to develop a multi-tracer approach based on a suite of innovative isotopic tracers sensitive to nutrient sources and cycling to be determined in snow, sea ice and seawater obtained from the year-round MOSAiC initiative and from multiple summer cruises to the Arctic Ocean. By combining established and new analytical techniques in trace element and isotope geochemistry and by conducting laboratory-controlled sea-ice growth experiments, this innovative and interdisciplinary approach will take advantage of cutting-edge knowledge in tracer development and behavior without compromising on the benefit of a seasonally and spatially constraint dataset. The results will provide a deep understanding of the effects on - and feedbacks within – Arctic marine ecosystems arising from
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Tanycyte/arcuate Neuron communications in the regulation of energy balance' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

The escalating pandemic of obesity and type-2 diabetes represents one of the most pressing and costly biomedical challenges confronting modern society. The brain's impaired ability to sense and respond to variations in energy homeostasis is increasingly recognized as playing a role in the pathophysiology of these disorders. By sensing the nutritional status of the organism, distinct neural cell populations –including neurons and glia present in the arcuate nucleus of the hypothalamus (ARH)– tightly regulate energy balance. In the last decade, it has become clear that tanycytes –hypothalamic glial cells lining the bottom of the third ventricle and sending a single basal process into the ARH– are also able to detect variations of nutrient and hormone levels. Henceforth described as “sensors” of the metabolic state, tanycytes would then modulate neuronal function in order to regulate energy balance. However, the mechanisms underlying the communication between tanycytes and ARH neurons in the context of metabolism remain largely unexamined.The overall objective of this project is to elucidate the molecular, structural and functional organization of tanycyte/ARH neuron communications, and their involvement in the regulation of energy balance. A multidisciplinary approach including neuroanatomy, molecular biology, and physiology will be implemented to achieve four specific aims: 1)To decipher the genetic and neuroanatomical features of tanycyte/ARH neuron metabolic units2)To ascertain the impact of tanycyte Annexin A1 on ARH neuronal function3)To uncover the role of tanycyte local translation in specific tanycyte/neuron communications4) To reveal the impact of tanycyte glucose-sensing on ARH neuronal gene expressionThe elucidation of tanycyte/neuron communications will be ess
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Quantitative analysis and modeling of the effect of human-induced land use/-cover change on the rate and spatial pattern of desertification in the Mediterranean using novel geochemical techniques' (COSMERO) under Sixth Framework.

assistant: **Objectives**

Human-induced land use/-cover changes are causing adverse effects on the life quality of the physical environment, thereby affecting prospects for future development. Although the long-standing interest in the interaction between land use/-cover change and geomorphic processes, our present-day understanding is far from complete. At present, models do not yet allow quantifying the impact of land use/-cover change, as they are calibrated for single erosion processes using short-term erosion data, representing a combined signal of both natural and human-induced erosion. The use of novel geochemical techniques, specifically in-situ produced cosmogenic nuclides (ICN), is seen as offering a means of overcoming some of these problems. The technique was first applie d in erosion studies ten years ago, and has proven to be remarkably accurate to provide long-term erosion rates. The aim of this project is to quantify the effect of land use/-cover change on the pattern of geomorphic processes using ICN. First, it aims to clarify the effect of the environmental setting of the area where sediment is eroded on the cosmogenic isotope content of river deposits. Once the basic principles are clarified, the technique will be applied to a selected number of catchments within the Mediterranean to quantify the impact of land use/-cover change on desertification. These data will then be used to calibrate desertification models. This project will contribute to strengthen the scientific understanding of processes controlling desertific ation, and is thereby directly relevant to ‘Mechanisms of desertification and natural disasters’ and particularly to ‘Assessment of the vulnerability to desertification’ within 6th Environment Action Program. The quantitative data on anthropogenic acceleration o
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'New Exploration Tools for European Pegmatite Green-Tech Resources' goes beyond current state-of-the-art under Horizon 2020.

assistant: **Innovation**

The manufacture of devices for green energy production and storage in Europe is a strategic, fast-growing sector which is essential in ensuring that the EU meets its energy and climate targets for 2030. It is worth an estimated €30 Bn in turnover, with investments of €4 Bn in the EU27, and is likely to create c.a. 100,000 jobs over the next 10 years. A major limitation to this is that 95% of the key raw materials for green energy devices are currently imported from outside the EU. Securing domestic deposits is therefore urgent for sustainable industrial development, mainly in retaining a large part of the added value, reducing supply risks and ensuring EU environmental standards for the production of raw materials. Exploration investment in Europe has declined in recent years due to increased technical demand and socio-political debate. Private sector engagement will increase only when technical solutions allow economically viable and environmentally friendly exploration and mining. Geologically, lithium-caesium-tantalum (LCT) and niobium-yttrium-fluorine (NYF) pegmatites are relatively common in Europe, enriched in many CRM needed for energy technologies but difficult to explore because most are buried, small and clustered. The GREENPEG approach will develop and test a set of high-level exploration technologies and algorithms to be integrated and upscaled into flexible, ready-to-use (TRL 7) toolsets for the identification of buried pegmatite ores. Validation of the new approach will be ensured from industry-led trials at locations in Austria, Ireland and Norway, while application studies will also be done in Finland, Portugal and Spain. Many of these target areas have established downstream processing industry, thus extending their value chains. The data acquired will
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'High throughtput electrophysiological measurements coupled with transcriptomics to reveal cellular dysfunction in type 2 diabetes' (T2D-EOMICS) under Horizon Europe.

assistant: **Objectives**

In this project, I will develop methods to simultaneously measure transcriptomes with single-cell resolution and perform high-throughput functional measurements of individual pancreatic islet cells. I will focus on - and -cells which regulate glucose levels by secreting the two main glucoregulatory hormones -insulin and glucagon- and whose dysfunction is associated with diabetes. The islet is a highly heterogenous mini-organ, making it an ideal tissue to study the relationship between molecular and functional heterogeneity. It has been recently developed the use of patch-clamp electrophysiology and single-cell RNA sequencing (patch-seq) using whole-cell electrophysiology. In this work, I will combine high-density microelectrode array technology (HD-MEA) and transcriptomic methods to identify changes that lead to dysfunctional cellular states in type 2 diabetes. To do so, I will build a cell cherry-picking system to perform single-cell RNA sequencing after electrophysiological measurements in the same islet cell. Then I will use this system to investigate subpopulations of - and -cells that have been previously identified in human islets from donors with type 2 diabetes using transcriptomic methods, but whose implications in cell and tissue function are yet unclear.


**Keywords:** Electrophysiology, Diabetes, single-cell, islet cells
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Understanding the identity of the amniotic fluid stem cells' (AmnioticID).

assistant: **Innovation**

The amniotic fluid surrounds and protects the foetus during development, providing trophic and mechanical support to its growth. This liquid contains a variety of cells shedding from embryonic and extra-embryonic tissues that have long been utilised for prenatal diagnosis. In a seminal 2007 article, Professor De Coppi identified in the amniotic fluid, a small population of cells with broad multi-lineage differentiation ability defined as Amniotic Fluid Stem Cells (AFSCs). AFSCs can be isolated, expanded and differentiated during gestation, making them ideal  for the development of pre-/perinatal autologous regenerative medicine strategies. In the last decade, a series of studies focused on investigating the therapeutic relevance of the AFSCs for various tissue compartments, such as skeletal and cardiac muscle, lung and liver. However, very little is known about the AFSCs origin, lineage identity and role during human development. This knowledge gaps have relevant impact on these cells' clinical translation and a list of important questions remains to be addressed: Which tissue releases the AFSCs? Are these cells constantly generated, or part of a self-renewing niche? What is the function of the AFSCs during human development? This proposal aims at addressing these questions, by using a combination of next generation sequencing analyses, cell biology assays and in vivo linage tracing approaches. Ultimately defining the origin and identity of the AFSCs will not only increase our knowledge of their physiological role, but will also allow to better direct future studies on their therapeutic implications. Thanks to this project, I will gain a comprehensive, multi-faceted understanding of the biology and regenerative potential of AFSCs. This will open up new research agendas,
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Networking Centres of High Quality Research on Knowledge Technologies and Applications' under Sixth Framework.

assistant: **Concept**

The project promotes the co-operation of educational and research institutes in area of knowledge technologies and their applications in eLearning, and accelerates the dissemination and application of the research results in associated candidate countries. The current cooperation between the partner institutions will be enhanced by means of visits, working meetings and joint workshops for presenting and analysing their research outcomes, experiments and best practice solutions. The project will increase the societal impact of research results in the field of knowledge technologies, such as semantic web techniques, data and web mining, multiagent technologies, embedded intelligence approaches etc. It will exploit the educational potential of the embedded intelligence applications and the integration of web, digital TV and mobile technologies. The integration of high quality research institutions for exchange of research information, results and personnel in priority IST areas (Semantic-based knowledge systems, Technology-enhanced learning) will be an effective instrument for dissemination and deployment of knowledge intensive applications in the partner ACC in their transition to the Knowledge Society. Furthermore, the project promotes applications of the partners research results in production of the multimedia-based educational materials, permitting interoperability between different e-Learning platforms and multilingual re-usability of eLearning content in different language environments.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Euro Summer School on Electron Crystallography 1,2 & 3' (1998-2002).

assistant: **Innovation**

The schools provide the necessary theoretical background for understanding the physical process of electron scattering and imaging and give an introduction to the recent developments in theory and techniques for the determination of crystallographic structures by transmission electron microscopy (TEM) imaging and diffraction. Examples to be discussed range from inorganic materials in the field of technological development to applications of the techniques to polymers, organic crystals and biological macromolecules.The lectures and training sessions will be given by internationally recognised experts to make sure that the diverse fields are introduced as follows: Image formation and diffraction; kinematical and dynamical theory; symmetry in real and reciprocal space; experimental techniques and detectors for data collection; data processing of HREM-images with Fourier processing and CTF-correction; evaluation of CBED patterns; 3D datamerging; phasing methods; constructing structure models in 2D and 3D; structure refinement and verification; analysis of defects and interfaces.Each of the three planned EURO Summer Schools consists of four different parts: a set of basic lectures, a set of advanced lectures, a set of practical sessions and a poster session of the participants.It is planned that the same speakers will give the basic lectures in the three Summer Schools. Varying speakers will be invited for the lectures on advanced and complementary subjects for the three different Summer Schools. All speakers are involved in state-of -the-art research and can give lectures about present issues in the various directions.ftp://ftp.cordis.lu/pub/improving/docs/HPCF-2000-00187-2.pdf