RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Deciphering and targeting cellular states in glioblastoma' (2021-2027).

assistant: **Innovation**

Cellular heterogeneity is a fundamental property of glioblastoma (GBM) tumors and presents a major barrier for therapeutics. During the past six years we characterized this heterogeneity in glioblastoma, and in other types of glioma, using single cell RNA-seq (scRNA-seq). We found consistent patterns of heterogeneity across patients, such that each tumor harbors multiple subpopulations of cells that resemble neurodevelopmental cell types. The consistency of these cellular states across patients highlights their potential clinical significance. Yet, our understanding of how these common states are regulated and of how distinct states respond, individually or in combinations, to potential treatments, remain poorly understood. Furthermore, it is unclear if additional states of clinical significance remain to be uncovered. To address these challenges, we will perform extensive studies of GBM patient samples, animal models and gliomasphere culture models. First, we will dissect the regulation of the common cellular states (Aim 1). Single cell ATAC-seq of patient samples will be used to infer transcriptional regulators, Spatial Transcriptomics will be used to infer environmental interactions, and resulting predictions will be tested further in established model systems. Second, we will search for novel cellular states, including rare states or those specifically associated with invasion to the brain parenchyma (Aim 2). Third, we will examine strategies for treatment of heterogenous tumors that are composed of multiple states (Aim 3). We will screen for state-specific drug sensitivities, as well as for drugs that induce state transitions, and develop rational combinations to eliminate multiple co-existing states, while considering interactions among states. Taken together, the
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'St. Bernard - Emergency EEG and auditory evoked potentials for earlier diagnostics and treatment planning of unconscious patients' (2014-2020), addressing dissemination and exploitation.

assistant: **Impact**

An ambulance crew is dispatched to help an unconscious man lying in the street. Is he having a stroke? Is he haemorrhaging in the brain? Has he hit his head? Is he an epileptic? Should the crew start thrombolytic treatment, stabilize the patient or give benzodiazepine? What next? These decisions need to be made every minute, every day around the world by first aid response units.Brain-related injuries and dysfunctions cause high costs to society. The earlier the patient can be diagnosed, the more optimised the treatment path is, and the treatment outcome improves. All this saves money and time in one of the most expensive treatments and rehabilitation.Mega Electronics has developed a solution for emergency brain (EEG) monitoring and plans to develop it into a new business concept. The concept allows easy-to-use, sub-hairline emergency EEG and auditory evoked potential (AEP) recordings and continuous monitoring during the entire patient care chain from emergency scene to hospital. The recorded EEG signals (and biomarkers extracted from them) can be visualized and monitored both locally and remotely, even during medical imaging. Previous studies have proven excellent signal quality. Future challenges lie in expanding the signal information content while maintaining ease-of-use for emergency situations and in inclusion of AEP monitoring.The product consists of an innovative, easy-to-use electrode strip, wireless transmission module and a remote monitoring system. No equivalent solutions are in the market yet. Users are medical professionals working in ambulances, hospitals and health care centres.Feasibility assessment (phase 1) will develop the business concept, study the benefits of AEPs in emergency medicine, plan the technical modifications needed to current products,
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Strengthening of scientific excellence of the National Veterinary Research Institute in animal health and food chain safety' (VET-TWIN) under Horizon 2020.

assistant: **Objectives**

The general objective of the VET-TWIN project is to increase the potential and research capacity of the National Veterinary Research Institute (NVRI) by cooperation with internationally-leading counterparts from Germany (Federal Institute for Risk Assessment-BfR) and Denmark (Technical University of Denmark, National Veterinary Institute; DTU Vet) − to act in an international scientific and research environment as a leading institute in the fields of animal infectious diseases, zoonoses and food chain safety.The objective defined in such a way will be accomplished through a number of activities, in particular through the implementation of the Joint Strategy of Cooperation and Development, and by introduction of mechanisms for the interaction of scientists at the operational level, as well as through activities helping in the exchange of knowledge and experiences among scientists.The concept of the VET-TWIN project fully addresses the objectives of the Horizon 2020 programme. Through activities planned in order to increase the level of scientific excellence of the institute and its research staff, the project fully implements the specific objective Spreading Excellence and Widening Participation. The Implementation of the project fits directly in with the specific challenge of Twinning. By creating a partnership between the aforementioned entities, it will be possible to cooperate and exchange knowledge on an international level. Furthermore, the involvement of two scientific centres of excellent repute in the international research community to the VET-TWIN project, will prevent the crowding-out effect for an institution from a country with a lower Composite indicator of Research Excellence.The above-mentioned factors justify the need for the implementation of the proje
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Optimizing Polyhydroxyalkanoate Production Through Macro and Molecular Insights in Mixed Microbial Cultures' (SalinePHA) targeting Horizon Europe.

assistant: **Abstract**

Plastic waste is a significant global environmental challenge due to its environmental persistence and the threat that microplastic poses on living organism. Polyhydroxyalkanoates (PHAs) are biopolymers produced by prokaryotes under availability of excess carbon but unfavorable growth conditions. Interestingly, certain types of stress conditions have been proven to increase PHA production in axenic cultures such as oxidative stress or osmotic upshock. PHA stands as one of the main alternatives to traditional plastics as they are biodegradable under a wide range of conditions, but high production cost hampers its wide adoption. Current technologies are based on the use of mixed microbial cultures (MMCs) in open bioreactors feed with industrial by-products to reduce the production cost. However, the PHA productivity using these approaches is low compared to axenic cultures growing on defined media. SalinePHA aims to enhance PHA productivity in bioreactors by using halophilic MMCs. Availability of metagenomic and transcriptomic techniques provides a great opportunity to study the genetic regulation of the PHA synthesis/depolymerization and potential shifts of the metabolism in complex microbial communities under different types of stress.  To achieve this, i) halophilic PHA-accumulating MMCs will be obtained by growing environmental inocula under selective pressure and, characterized (performance parameters and shotgun metagenomic sequencing); ii) RNA-seq, RT-PCR, 2D-DIGE experiments will be performed on the best PHA-accumulating MMC under various stress conditions to understand the PHA metabolic regulation. A deeper understanding of the PHA accumulation dynamics will allow for the design of operational parameters by using stress conditions as elicitors to induce high PHA-
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'ICFOstepstone PhD Programme for Early-Stage Researchers in Photonics' (ICFOstepstone).

assistant: **Innovation**

ICFOstepstone is being proposed as an evolution of the current PhD-programme, offering competitive fellowships, paired with dedicated training actions within the chosen research field as well as for acquiring transferable skills, implementing mobility-actions in form of compulsory secondments at partner institutions in academia and industry alike, aiming at an integrated PhD aligned with the objectives in H2020.We aim at promoting an international research environment where traditional boundaries between fields and sectors are crossed and multidisciplinary projects undertaken, therefore aiming at covering the triple “i” dimension. It is expected that the award of a Fellowship under the proposed Programme will significantly boost the Fellow’s career perspectives and their connections, enabling a diverse career path in the most important institutions worldwide of their chosen sector, therefore guaranteeing a smooth and successful transition to their next career step.The ICFOstepstone is considered to be a natural advancement from the COFUND programmes ICFO launched in the past, in terms of now expanding our efforts to the postgraduate level, to establish a long-term talent pipeline at any career level. This COFUND action therefore proposes a change in the focus group of potential recipients from the Experienced Researchers to Early Stage Researchers. Also, with ICFOstepstone, the excellence approach not only aims at an enhancement of the Recruiting & Hiring Life-Cycle but also at an enhancement of the Career Development Plan.As shown in the proposal, ICFO has the necessary expertise to implement and develop the programme due to its broad and proven track record in the management of international research and education programmes. The proposal is also in line with current
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Mechanism of nucleosome assembly during DNA replication' under Horizon 2020.

assistant: **Concept**

Proper inheritance of the epigenetic information during cell division controls cell fate decisions, tissues homeostasis and development, ensuring disease avoidance. We have little understanding however of the mechanisms by which epigenetic information, specifically histone post-translational modifications in nucleosomes, are replicated in parallel to the DNA prior to cell division. This process strictly depends on proper nucleosome formation on the newly synthesized DNA strands. Here, I will study the molecular mechanism of nucleosome assembly during DNA replication. Nucleosome dynamics during DNA replication is controlled by an interconnected network of histone chaperones that converges on the key Chromatin Assembly Factor 1 (CAF-1). I recently elucidated the molecular mechanism of CAF-1-mediated nucleosome assembly, in absence of any other replication components. I developed a quantitative (NAQ) assay that allows, for the first time, the quantification of nucleosome assembly activity in vitro. In cells, CAF-1 is recruited to replication forks by the DNA polymerase processivity factor PCNA. Here, I will capitalize and expand on the assays above to integrate structural data, quantitative biochemical and biophysical measurements, and functional analyses, to elucidate how CAF-1 crosstalks to PCNA, DNA polymerases and other components of the DNA replication machinery in S phase. Specifically, the proposed research will 1) uncover how CAF-1 recruitment by PCNA affects its function in nucleosome assembly, and 2) examine how CAF-1 activity is regulated during ongoing DNA replication.  This work will reveal the mechanism of nucleosome assembly during DNA replication and its interplay with S phase signaling. A mechanistic understanding of this pathway will uncover the fundament
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'A Comparative Diachronic Analysis of Post-Byzantine Networks in the Early-modern Europe (15th-18th c.)' under Horizon 2020.

assistant: **Concept**

The research proposed here will focus on the connections of post-Byzantine émigrés to the early-modern European West. The Researcher will examine how these webs were organized, controlled and enacted to keep together émigrés of various backgrounds, generations and interests for longer periods of time and in changing historical circumstances; then, it will survey how the nods established by these bonds facilitated interactions between the émigrés and their host societies; lastly, it will analyse the diffusion and “behaviour” of these networks, seeking significant mechanisms and potential common patterns that channeled the key integration processes in a wider context of early-modern Europe.The core part of the research will be done in the Host Institution's premises; apart from its resources, the Researcher will use extensive library collections and archival materials located in institutions related to the Host Institution, or the ones that generally sustain scholarly research in the humanities.  The research will be followed by intense training and networking that will allow efficient transfer of knowledge. From the Host Institution, this transfer will aim at enhancing the Researcher's capacities in working with latest theoretical and technological tools, which will, consequently enhance the visibility of her work in the international field academia, as well as a further transfer of this knowledge and specific research outcomes to the related projects and regions of EU with which the Researcher is or will be collaborating. The Researcher, from her end, will transfer her field expertise in working with CE and SEE field contexts, as well as her skills in publishing, program development and coordination, and field networking.Among outcomes of this collaboration will be scho
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'EVOLUTIONARY MECHANISMS OF AGE-RELATED BONE LOSS ACROSS THE HUMAN AND HOMINID SKELETON' in the context of a Horizon Europe call.

assistant: **Objectives**

Age-related bone loss in humans is widely documented and linked to the process of skeletal gracilization, evolved over the last 10 kyrs. One explanation for the human susceptibility to bone loss during ageing is that osteoporosis is an unintended consequence of otherwise advantageous evolutionary adaptations. However, the deeper evolutionary roots and potential epigenetic changes that underlie the biological mechanism of age-related bone loss in humans compared to other hominids are still not understood. Osteoporosis is indeed a global health issue affecting millions of people worldwide with greater prevalence in post-menopausal women. Despite the clinical and biosocial implications of the disease that particularly impact public health issues related to women’s lives, an understanding of the evolutionary roots of osteoporosis is conspicuously lacking. STAND UP aims to fill this gap by identifying and quantifying patterns of age-related bone loss across the skeleton in humans and a comparative sample of our closest relatives, chimpanzees and gorillas, and identifying its epigenetic correlates across taxa. This project will take a novel, comparative and interdisciplinary approach by combining (1) a holistic investigation of the skeletal changes in internal and external bone morphology across the skeleton in unique skeletal samples of humans, chimpanzees and gorillas that each come with critical, individualized demographic and behaviour data, and (2) a comparative molecular analysis of epigenetic bio-markers that underpin patterns of skeletal aging. Ultimately, STAND UP will combine functional morphology of the skeleton, molecular biology and evolutionary anthropology, together with expertise in clinical medicine, to clarify how and why bone structure changes through adult
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Cloud-based Rapid Elastic MAnufacturing' (CREMA) under Horizon 2020.

assistant: **Objectives**

Due to the proliferation of ICT Technologies, manufacturing industry is undergoing substantial transformation in terms of HW but also in terms of Cyber-Physical Production Systems and the SW and services used within production environments. In parallel, the manufacturing processes of the future are changing and need to be highly flexible and dynamic in order to satisfy customer demands for, e.g. large series production, mass customization, or changing orders.To keep pace with the needs of the manufacturing industry of the future, in Manufacturing 4.0 companies need to flexibly react to these demands and be able to offer production capacities in a rapid way. Thus companies looking for manufacturing capacity need to be supported by the means to find these capacities, configure them, and integrate them into their own manufacturing processes.To achieve this, one obvious approach is to port successful concepts from the field of Everything-as-a-Service (XaaS) and Cloud computing to manufacturing to mirror agile collaboration through flexible and scalable manufacturing processes: • Leasing and releasing manufacturing assets in an on-demand, utility-like fashion• Rapid elasticity through scaling leased assets up and down if necessary• Pay-per-use through metered serviceApplying these principles, Cloud manufacturing can move manufacturing processes from production-oriented to service-oriented networks by modelling single manufacturing assets as services in a similar way as SaaS or PaaS solutions. By modelling all process steps and manufacturing assets as services it is possible to realize cross-organization manufacturing orchestrations and integrate distributed resources and ultimately manufacture products more efficiently.While the theoretical foundations for Cloud manufacturin
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Seventh Framework proposal on 'Comparative studies of inbreeding effects on evolutionary processes in non-model animal populations'.

assistant: **Concept**

Comparative studies of inbreeding and evolution in non-model animal populations: a research proposal directed towards integrating ecological and evolutionary research on inbreeding. Specifically, my aim is to apply novel ecogenomics tools in the study of evolutionary consequences of inbreeding in non-model animal populations. At present, our understanding of inbreeding is dominated by studies of a small number of model organisms. I will undertake comparative studies on inbreeding effects in a genus of spiders containing independently evolved naturally inbreeding species as well as outcrossing sister species. The study of a naturally inbreeding animal species will provide unique insights to consequences of inbreeding for population genetic structure, genome-wide genetic diversity, and evolution of life history traits. Social spiders are not only unique because they naturally inbreed, but also by being cooperative and showing allomaternal brood care including self-sacrifice, and they evolve highly female-biased sex-ratios, a trait that is not well understood in diploid species. My research objectives are 1) to establish a robust phylogeny for comparative studies; 2) to quantify the effects of inbreeding on the genetic diversity within and between populations; 3) to estimate gene flow among inbred lineages to determine whether inbred lineages diversify but retain the potential for gene exchange, or undergo cryptic speciation; 4) to determine effects of inbreeding on gene expression; 5) to investigate the mechanism underlying the genetic sex determination system that cause female biased sex-ratios; and finally 6) to determine whether sex-ratio is under adaptive parental control in response to genetic relatedness and ecological constraints. Addressing these objectives will g