RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Development of improved strategies for the control of faba bean necrotic yellows virus - fbnyv - in food legume crops of West Asia and North Africa' (1994-1998), addressing dissemination and exploitation.

assistant: **Impact**

The main objectives are as follows:* To conduct field studies of FBNYV build-up and spread in Egypt and Syria to obtain a better understanding of the factors involved in virus survival and spread under field conditions.* To develop screening methods which will permit the precise determination of virus resistance levels in the existing faba bean, chickpea and lentil germplasm.* To determine the incidence, distribution and variability of FBNYV in countries of West Asia and North Africa (WANA) by providing diagnostic tools to collaborators of the existing ICARDA network.* To characterize the FBNYV genome by infectivity tests, by determining FBNYV gene functions and variability as well as by testing in vitro engineered replicase-associated protein (Rep) variants of FBNYV in a single cell-based replication assay for their capacity to inhibit the replication of wild-type FBNYV.* To use in vitro engineered Rep genes for generating transgenic faba bean plants and determine the levels of virus resistance in these lines.Expected OutcomeThe work carried out in this project should provide an updated overview on the incidence, distribution and variability of FBNYV in WANA countries and give a better understanding of FBNYV ecology and epidemiology. This information will be essential for devising agricultural practices for FBNYV control. Improvement of the screening methodology and evaluation of FBNYV resistance in available germplasm will lead to the use of host resistance to FBNYV as another means of conventional control. The analysis of the FBNYV gene functions and variability and the design of defective interfering Rep protein variants will hopefully lead to the generation of transgenic faba bean plants with high levels of FBNYV resistance. The project will thus contribute to sust
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Roots of Communication' demonstrating excellence, impact and implementation quality for a Horizon 2020 call.

assistant: **Abstract**

The ability of the human species to share information with others is unique due to the fascinating expressivity of human communicative actions. The primary aim of the present proposal is to investigate the biological basis of this ability to reveal 1) the evolutionary foundations and 2) the neural underpinnings of it, which makes humans able to understand the meaning of communicative signal sequences.To test the first question, I will investigate whether a non-human animal species can recognize and prefer acoustic signals that are indicative of communicative information transfer in domestic chicks. To examine the second question, I will conduct two EEG experiments with young human infants to find specific neural markers of communication and to prove that communication can elicit high-level inferences about agency. In all experiments, I will rely on my recent results published in high-ranked, multidisciplinary journals, which show that the variability of signal sequences in turn-taking interactions is interpreted by human infants as indicative of communicative information transfer – even if the signals and the communicators are unfamiliar to them.The Centre for Mind/Brain Sciences and the supervision of Professor Vallortigara offers the perfect infrastructure and professional background to investigate both the comparative and the psychophysiological questions of the proposed project at the same time. Thus, the examination of the neural underpinnings of the sensitivity to communicative information transfer and the evolutionary ancient cognitive mechanisms behind it will enable to understand the biological foundations of human communication, which is not possible without testing non-linguistic and pre-linguistic participants. The findings of the present proposal can be hig
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon 2020 proposal for the project 'Priorities for Addressing Opportunities and Gaps of Industrial Biotechnology for an efficient use of funding resources'.

assistant: **Objectives**

The Progress project will support and accelerate the deployment of IB in the EU indus-try by identifying high-value opportunities for IB and proposing actions to address them successfully. The CSA will conduct a multidisciplinary approach with integration of desk re-search, statistical modelling, qualitative assessment and synthesis of data and stakeholder information and perspectives on a detailed level, in order to provide good and non-biased understanding and adequate knowledge for action. The CSA will consist of three phases:First, a comprehensive and dependable information base which allows for plausible estimations on the future of IB in the EU in the short and medium-term will be provided. It consists of an integrated analysis that considers technological, societal, political, de-mand, acceptance dimensions. Therefore, quantitative (indicators, modelling) and qua-litative information will be combined. In order to identify the R&D&I needs that are con-gruent to the European capacities and to delineate adequate actions the CSA will adopt an international value chain/ecosystem perspective and provide an in-depth analysis of the most relevant value chains. Second potential desirable, realistically achievable future paths for the various value chains for IB will be elaborated. These will eventually be combined into an integrated vision for the future of IB in Europe by engaging with the different stakeholder commun-ities.In the third phase high-value opportunities for IB and related R&D&I needs will be de-ducted and prioritized. This will provide guidance on need for action to build up and focus national/regional capacities and activities on key segments. Moreover, it will pro-vide strategic guidance for the formation of an integrated European technology base. We will
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Identification of molecular targets of psychoactive kavalactones using iBodies' (KavaTarget), addressing dissemination and exploitation.

assistant: **Impact**

Plant natural products have traditionally provided a great source of chemical scaffolds for the development of new medicines. Following a downturn in the late 20th century, natural product research is now experiencing a renaissance owing to rapid developments in genomics and metabolomics technologies. However, the identification of protein targets of newly discovered bioactive natural products remains very low-throughput. Under this proposal, I will carry out research to develop a new methodology for the elucidation of molecular targets of small bioactive molecules that bind to human proteins such as membrane receptors, using stochastically-generated polymeric probes called iBodies combined with horseradish peroxidase-mediated biotin labeling. In particular, I will focus on psychoactive metabolites from kava (Piper methysticum) called kavalactones, which have well-documented anti-anxiety properties, but their mechanism of action is unclear. In the first stage, I will develop the molecular target identification workflow using a previously characterized ligand, resiniferatoxin, which binds with high affinity to the human pain receptor TRPV1. Once the workflow is developed, I will proceed to identify the brain receptors that are the primary targets of natural kavalactones, as well as non-natural kavalactone derivatives that I recently developed. In addition, I propose that a similar approach can be applied to identify liver cytochrome P450 enzymes that are inhibited by natural products such as kavalactones. In the last stage of the project, I will develop a protocol for the extraction of liver proteins and identification of liver enzymes that interact with kavalactones. This research will deliver a new biochemical method for molecular target identification in the short ter
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Role of different tafs containing complexes in regulation of gene expression' (2002-2006), addressing dissemination and exploitation.

assistant: **Impact**

In my proposal I plan to investigate the role of TFIID and novel TAF-containing complexes in the regulation of gene expression and to highlight differences between the distinct complexes in transcription regulation.The objectives of my proposal are centered around dissection of TFIID, TFTC, STAGA and P/CAF specific role(s) in genes expression regulation and my objectives are the following:-Identification of TFIID, TFTC, STAGA and P/CAF target genes. Do these complexes regulate different promoters or act on the same promoters with different functions that could be partially redundant or not? Or are different combinations of them required to fulfil gene regulation in the complexity of human genome?-Dissection of their mechanisms and dynamics in transcription regulation. Promoter's histones acetylation is an early event during gene activation, since three out of four of these complexes contain a histone acetyl transferase enzyme, it is crucial to understand if these activities play the same role in different contexts or if they have different targets and then which is the meaning of target acetylation.To answer these questions I plan to use the following approach: RNA interference of different subunits: common to all complexes, shared by some but not all of the complexes and complex scientific. The comparison of gene expression profiles, obtained with cDNA arrays, by subtractive analysis of the different samples will permit the creation of categories of genes regulate either by different, or combinations of complexes.On some of these genes a more detailed analysis will be performed using chromatin immunoprecipitations to elucidate the interplay of these factors with the other components of the transcription machinery. The accomplishment of these objectives will clarify imp
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Does a moving hybrid zone leave a genomic footprint?' in the context of a Horizon 2020 call.

assistant: **Objectives**

Species are the currency of biodiversity. However, during the early stages of their evolution, species are difficult to delineate, as barriers to gene flow are not yet complete. Range expansions and contractions exaggerate the reshuffling of the genomes of hybridizing species. The key objective of this project is to experimentally test the theoretical prediction that, under hybrid zone movement, genes derived from a displaced species are left behind in the genome of an invading species, leaving a ‘genomic footprint’. I will fill an empirical void by, for the first time, testing this critical hypothesis in the wild, using genome-scale data. I have established a study system (the newt genus Triturus) that is particularly appropriate because it shows strong evidence in support of hybrid zone movement over considerable time and distance. To obtain genome-wide data, I will be trained in the cutting-edge genomic technique of target enrichment through sequence capture during the outgoing phase at the lab of Prof. Shaffer at the University of California, Los Angeles (UCLA). To transfer this important new piece of knowledge back to Europe, I will implement target enrichment through sequence capture during the return phase at the beneficiary, the University of Sheffield (USFD). At USFD the group of Prof. Butlin has unmatched expertise in hybridization/speciation research and excels in the analytical approaches required to uncover a genomic footprint. Human activities have intensified hybridization and I propose a secondment to work together with conservationists and legislators to tackle the insidious conservation problem of genetic pollution by exotic species.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'The role of histone chaperones during epigenetic reprogramming' in the context of a Seventh Framework call.

assistant: **Objectives**

During the life of an individual, large scale epigenetic reprogramming occurs twice: in zygote, shortly after fertilization and during the development of primordial germ cells. We have previously shown that both reprogramming events entail large scale chromatin remodelling connected with dynamic changes in the abundance of histone variants. Epigenetic reprogramming in germ line is characterized by the concomitant loss of histone variant H2A.Z and H3K9me3 and H3K27me3 histone modification marks. During the reprogramming in zygote, histone variant H2A.Z also disappears and there is a selective early incorporation of H3.3 histone variant into paternal chromatin. Histone chaperones are responsible for the incorporation and removal of histones, and Nap1l1 and Hira have been found enriched during epigenetic reprogramming in vivo. In the proposed project, we will generate Nap1l1 and Hira conditional knockout mouse models to investigate the exact role of these factors in epigenetic reprogramming in the developing germ line and in zygotes, respectively. Furthermore, using our genetic models, we will address the involvement of these chaperones in the development of early germ cells as well as in oocyte maturation and during early mouse pre-implantation development. Last but not least, we will also investigate the importance of Hira and Nap1l1 in the reprogramming processes in vitro - during nuclear transfer (SCNT), in cell fusion and iPS generation. Our findings will provide novel insights regarding the role of histone dynamics during epigenetic reprogramming and will add to our understanding of the molecular mechanisms underlying reprogramming processes. Epigenetic reprogramming plays pivotal role in the dedifferentiation and the reversal of cell fate decisions, and hence the pr
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Mixed Hodge realization of triangulated mixed motives and comparison of triangulated categories of motives' (2002-2006), addressing dissemination and exploitation.

assistant: **Impact**

In the 1960s Grothendieck made conjectures as to the existence of a universal framework for cohomology of algebraic varieties called the category of mixed motives. During the 1980's the conjectural formalism was enriched by conjectures of Deligne, Beilinson, Bloch and others. In the 1990's V. Voevodsky, M. Levine and M. Hanamura have given several constructions of triangulated categories that have many of the expected properties of the derived category of mixed motives. As part of Grothendieck and Beilinson 's conjectural formalism comparison of those theories with a particular derived Bloch-Ogus theory should be made via realization functors.The aim of this proposal is to construct the Hodge realization functor for Voevodsky's triangulated category of mixed motives over a smooth scheme of finite type over C and to construct equivalences between the triangulated categories of Voevodsky, Levine and Hanamura.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Automated selection by phage display of recombinant T-cell receptor molecules specific for melanoma-associated HLA antigen/peptide complexes' to be submitted under Fourth Framework.

assistant: **Abstract**

Objectives: To allow the exploitation of molecular immunological technologies to develop commercially interesting recombinant reagents for tumour diagnosis and therapy.Brief description:Using conventional radio- and chemotherapy, only limited success has been achieved, in terms of increased five-year survival times, over the past 25 years, for most cancer patient categories. Thus, the demand for new treatment modalities is overwhelming, and the potential market for new anticancer therapeutics is huge. The prospects for cancer immunotherapy rely on the identification of tumour-associated cell surface antigens and their specific recognition, e.g. by cytotoxic T Lymphocytes (CTL). Antigens recognized by tumour-specific CTL consist of peptides derived from endogenous proteins and presented by human major histocompatibility (HLA) class I molecules, ideally only on tumour cells. Several such polypeptides have been described in the past two years, e.g. in melanomas. However, despite their exquisite specificity, CTL have a number of shortcomings as compared to soluble molecules, in particular with respect to production, handling and general applicability. These difficulties may now be overcome by replacing tumour-specific CTL with recombinant receptor molecules (e.g. antibodies) recognizing HLA/peptide complexes. With phage display, such reagents can be made completely in vitro, bypassing the immune system and immunization procedures, and allowing in vitro tailoring of the receptor's specificity and affinity. As T cell receptors (TCR) are the recognition molecules of CTL, their soluble counterparts are the favoured molecular format for such reagents. However, the expression of TCR as soluble molecules was difficult and phage display libraries were not available.In order to over
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'NETwork of Stratospheric Platforms for Traffic Monitoring, Environmental Surveillance and Broadband Services' will create measurable scientific, societal and economic impact aligned with Fifth Framework.

assistant: **Impact**

The HeliNet project aims at designing an integrated network based on HALE (High Altitude Long Endurance) unmanned solar platforms. The main features of this infrastructure are its reconfigurability, flexibility, quick deployment, zero environmental and human impact. The HeliNet permits the exploitation of the stratospheric segment, and is suitable for several cost effective and interoperable applications in various strategic fields such as localisation, environmental surveillance, provision of telecommunication services. The project addresses all aspects of the HeliNet design. A stratospheric aerodynamic HALE platform, named HELIPLAT, is designed (included the solar and fuel cell energy subsystem), and a scaled size prototype is manufactured in order to perform static tests. The network design is carried out, considering various geographical coverage areas; a common communication interface is addressed, in order to guarantee the flexibility of HeliNet as for possible applications. Finally, the study of 3 pilot applications is performed, in the fields of localisation (including the possible integration with the Galileo system), environmental data processing and transmission, provision of broadband services. The project is carried out by a transnational and multi-sectoral partnership of research departments at universities and companies.Objectives: The objective of the HeliNet project is to create an integrated infrastructure based on HALE unmanned aerodynamic solar platforms. HeliNet contributes to ensure Europe a full role in the economic exploitation of the stratospheric segment and in the development of standards for the related applications. It contributes to EC scientific and technological development through the know-how, which is going to be acquired during the pr