RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Intrinsic autophagy receptors: identity and cellular mechanisms.' under Horizon Europe.

assistant: **Concept**

Macromolecular complexes (MC) are cellular machines that perform a wide array of vital tasks. They operate in a controlled, coordinated fashion within the crowded environment of the cell. Accumulation of dysfunctional MCs leads to age-related diseases. Despite recent technological advancements, it still remains elusive for many of them how excess or dysfunctional MCs are sensed and removed. I have recently established a role of intrinsic receptors in degradation of the nuclear pore complex and the clathrin-mediated endocytosis machinery by selective autophagy. Intrinsic receptors represent functional subunits of the macromolecular machine but can if needed recruit the autophagy machinery to engulf and degrade the complex. As such intrinsic receptors provide an in-built quality control function that monitors the assembly state and/or functionality of macromolecular machines.  I hypothesize that this is a conserved and widely used principle existing within various MCs and that there is a common, yet unexplored, regulatory pathway underlying the intrinsic receptors’ mode of action. In the proposed project, I will employ a combination of genetic screening, mass spectrometry and cryo-electron tomography to systematically define intrinsic receptors and their working principles in cells. I will use systematic discovery approaches to determine how many MCs contain intrinsic receptors, how the degradation of intrinsic receptors and their cargo is regulated, and how this is orchestrated with autophagosome biogenesis. My results will not only provide insights into a novel cellular quality-control mechanism but also unravel novel aspects of selective autophagosome biogenesis. Importantly, both accumulation of dysfunctional complexes and impairment of autophagy are linked to aging a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Gastrointestinal and neuropeptides regulating food intake in fish in response to dietary lipid composition' (GASTRONEUROPEP).

assistant: **Concept**

There is an urgent need to replace fish oil (FO) and meal (FM) from wild stocks in aquaculture diets with more sustainable alternatives, being vegetable oils (VO) the primary candidates. However, these oils are devoid of essential n-3 long-chain PUFA (LC-PUFA) and can be particularly rich in n-6 PUFA, leading to imbalances in the n-6/n-3 ratio. This situation is similar to what is presently observed in other animal production sectors as well as in human nutrition. It is relatively well established how these changes in dietary lipid composition might affect lipid metabolism but potential effects on the regulation of appetite and food intake mediated by gastrointestinal and neuropeptides are possible and have been hardly investigated. This will be the objective of the present project, which will employ an interdisciplinary approach combining traditional nutritional and physiological studies with molecular analyses to evaluate potential impacts of more sustainable feed formulations on the regulation of food intake, while also uncovering the mechanisms behind it. The knowledge gained from this project is of high scientific and socio-economical importance to the future development and expansion of the European aquaculture industry. In addition, if physiological mechanisms of regulation of appetite and satiety in fish are similar to those in mammals, as has been suggested, knowledge gained might be applicable to other animal production sectors and even human nutrition, including studies of nutritional disorders (e.g. obesity). Finally, the effects of FO replacement by VO in aquaculture diets on lipid metabolism have been the research focus of the candidate in the past 3 years. The present project would thus enable the transfer of knowledge and competence from a northern Europ
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Collaborative development and dissemination of workflows and techniques for using Correlative Light, Electron and X-ray Microscopy to progress research into the understanding and treatment of diseases' (CLEXM).

assistant: **Innovation**

Correlative multimode imaging is the only way to reveal a composite view of a biological sample with the multidimensional information about its macro-, meso- and microscopic structure, dynamics, function and chemical composition that is required in order to understand biomedical processes and diseases.Project CLEXM addresses an urgent need to demonstrate, promote and disseminate the benefits of this technique in the fields of disease and drug therapy research and especially to early-career researchers. The premise of project CLEXM is that there is a growing need for disease and drug therapy researchers to understand the linkages between structural and functional changes that occur in a cell and to be able to observe these from the cellular (micrometre) to the molecular (nanometre) scale. Correlative Light and Electron Microscopy (CLEM) is the current state-of-the-art for achieving this, but the technique is extremely complex and slow. CLEXM postulates that the integration of a third imaging modality, Soft X-ray Tomography (SXT), into CLEM will make it easier and faster for researchers to correlate cellular structure with cellular function. Correlative Light, Electron and X-ray Microscopy (CLEXM) can be combined in a number of ways and the benefits will be demonstrated in a number of different use cases. This would be too difficult and too much to achieve as a single research project or as a single MSCA Postdoctoral Fellowship, however, it lends itself to be most easily achieved as a network of complementary individual projects, under an MSCA Doctoral Network action.The overarching objective of project CLEXM is to provide high-level training in the field of correlative multimode imaging to a new generation of doctoral candidates to provide them with the transferrable ski
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Drivers and Brakes of CAR T Cell Efficacy Determined by the Tumor Immune Microenvironment' (CAR-TIME) targeting Horizon Europe.

assistant: **Abstract**

Cancer immunotherapies with chimeric antigen receptor (CAR) T cells have shown dramatic clinical efficacy in patients with B cell neoplasms. Thus, their clinical use is expected to increase considerably in the near future. However, for poorly understood reasons, not all patients with lymphoma benefit from these expensive therapies. The ability to stratify patients into probable responders vs. non-responders prior to immunotherapy will improve treatment efficacy, limit patient exposure to adverse effects, and mitigate the significant economic costs associated with these therapies.We and others have previously demonstrated that effective antitumoral immunity requires complex, spatially coordinated interactions between different cellular elements within the tumor immune microenvironment (TIME). There is evidence that patient response to immunotherapy is attributed to specific characteristics of the TIME, such as the composition, spatial arrangement, and activation states of immune cell types in it. Therefore, a better understanding of the TIME, and of how immunotherapies come into effect in live, intact human tissues, is critical for the selection of successful immunotherapies for our patients.The overarching aim of the CAR-TIME project is to explore and visualize the cellular and molecular mechanisms of CAR T cell efficacy in lymphoma, determined by CAR T cell interactions with the TIME. This shall be achieved by creating a high-dimensional map of the TIME of diffuse large B cell lymphoma, performing live tissue cultures treated with immunotherapies, and establishing a novel live cell microscopy platform to interrogate intact human lymphoma tissue treated with CAR T cells. Drug perturbations, multidimensional imaging technologies, RNA sequencing and integrative bioinforma
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'The evolution of social life and division of labour'.

assistant: **Objectives**

The hallmark of social insect colonies is reproductive division of labour which is often associated with dramatic morphological and behavioural differences between queens, workers and males. The aim of this proposal is three-fold. First, we will use our recently developed fiducial identification system to investigate the general principles of social organisation and division of labour. The video tracking of workers labelled with markers derived from the augmented reality library ARTag allows us for the first time to distinguish up to 2000 individuals and precisely locate them every 500ms, hence allowing large-scale experiments addressing the question of how the behaviour of individual workers is influenced by the joint effects of environmental factors and social interactions. The second related aim is to investigate how the level of altruism within colonies and the reliability of communication systems are shaped by colony kin structure. Because it is not possible to conduct artificial evolution with social insects we will use a new experimental system consisting of colonies of small mobile robots with simple vision and communication abilities. This system permits to conduct hundreds of generations of experimental evolution in colonies with variable group composition to identify the factors affecting the evolution of altruism and communication. Finally, we will complement these studies with a genetic perspective using a remarkable genetic social polymorphism that we recently discovered in the fire ant Solenopsis invicta. The advent of new ultra high-throughput sequencing techniques will allow us to document the steps involved in the evolution of this genetic social polymorphism and test the suggestion that the chromosome involved in the social polymorphism has the proper
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Fourth Framework proposal on 'Mathematical models for rhythmicity and social resilience in colonies of antleptothorax acervorum'.

assistant: **Concept**

Research objectives and content In Leptothorax colonies the individual ants tend to be active and inactive in synchrony. Leptothorax nests have a constant spatial structure. IndividuaLs show social resilience, that is they have spatial fidelity zones which they reconstitute in the same relative position when emigrate to new nest. The hypothesis whichto test is that rhe relative position of spatial fidelity zones is correlated with different leveLs of activity among the the workers. We shall develop both discrete and continous mathematical models for the system and we shall use the individual behavour to derive a continous model of the group properties. In sum, the goal ot this study will be to underderstand through cycles of modelling and experimentation how 1. rhythms occur when ants exhibit differential activity and occupy unique spatial fidelity zones 2. rhythms combined with social resilience may promote efficiency. Training content (objective. benefit and expected impact) Goal of the training is to start, within the above research project a collaboration between a biolooical approach and a mathematical approach. During this term I would to lem the techniques used in laboratory observation, images analysis and mathematical modelling. Therefore. I would attent specific courses of mathematics and modelling to improve my kwnoledge of mathematical models.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon Europe proposal on 'How Hands Help Us Hear'.

assistant: **Innovation**

Human communication in face-to-face conversations is inherently multimodal, combining spoken language with a plethora of multimodal cues including hand gestures. Although most of our understanding of human language comes from unimodal research, the multimodal literature suggests that hand gestures are produced in close synchrony to speech prosody, aligning for instance with stressed syllables in free-stress languages like English. Furthermore, prosody plays a vital role in spoken word recognition in many languages, influencing core cognitive processes involved in speech perception, such as lexical activation, segmentation, and recognition. Consequently, viewing gestural timing as an audiovisual prosody cue raises the possibility that the temporal alignment of hand gestures to speech directly influences what we hear (e.g., distinguishing OBject vs. obJECT). However, research to date has largely overlooked the functional contribution of gestural timing to human communication. Therefore, HearingHands aims to uncover how gesture-speech coupling contributes to audiovisual communication in human interaction. Its objectives are [WP1] to chart the PREVALENCE of the use of gesture-speech coupling as a multimodal prominence cue in production and perception across a typologically diverse set of languages; [WP2] to capture the VARIABILITY in production and perception of gesture-speech coupling in both neurotypical and atypical populations; [WP3] to determine the CONSTRAINTS that govern gestural timing effects in more naturalistic communicative settings. These objectives will be achieved through cross-linguistic comparisons of gesture-speech production and perception, neuroimaging of multimodal integration in autistic and neurotypical individuals, and psychoacoustic tests of gestura
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Economic assessment of European fisheries' under Fifth Framework.

assistant: **Concept**

General: to integrate and analyze the economic information available on European fisheries and to develop analytical tools in order to contribute to effective fisheries management.Specific:l. An Annual Economic Report (AER) on economic performance of selected fleet segments will be elaborated and submitted to STECF for review:1.1 Continuation of existing format;1.2 New fleet segments;1.3 EU-wide fisheries;1.4 Include Baltic area.2. Annual Economic Interpretation of the ACFM Advice (EIAA) will be prepared and submitted to STECP for review:2.1 Continue existing format;2.2 New fleet segments;2.3 Improve forecasting methods.3. Establish a data base for the compiled data.4. Develop analytical tools to exploit the data and allow improved estimations.The project PAIR CT97-3541 has established procedures for compilation of existing economic data regarding the performance of specific fishing fleets. The most recent report 1999 has been published by the EC in September 2000 (ISBN 92-828- 9600-5).
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Quantum Metrology Electrical Triangle' (QMET).

assistant: **Concept**

The standard definition of the Ampere can be obtained exploiting the dual counterpart of the Josephson effect, namely voltage Bloch Oscillations (vBO). A way to measure them consists in embedding a largely non-linear Josephson junction in a high impedance environment, irradiate it with a microwave pump, and observe discrete DC current steps. The QMET (Quantum Metrology Electrical Triangle) action tackles this objective by leveraging the researcher demonstrated ability of performing precision microwave measurements devising novel apparatuses and the mastering of the host group in the design and fabrication of high-impedance superconducting circuits. Such result would provide a leap in quantum metrology and paves the way to the redefinition of the ampere using only fundamental constants.


**Keywords:** Metrology
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Sequence-structure-function: uncovering how genetic variation at human centromere drives cellular phenotypes' in the context of a Horizon Europe call.

assistant: **Objectives**

The last decades have seen an extraordinary leap in our knowledge of the human genome and its role in health and disease. Yet, approximately 5% of our DNA still lacks sequence annotation and has been largely excluded from functional and disease-association studies. These genomic gaps include DNA repeats such as centromeres, which are large tandem arrays of alpha-satellite DNA. Centromeres chromatin is functionally essential for chromosome segregation serving as the basal template for the mitotic kinetochore. A recent breakthrough has been the complete genome assembly, including centromeres, of a haploid cell line derived from fetoplacental growth of a molar pregnancy. However, centromeres sequence and size vary across tissues, between individuals and in disease states. The main challenges are to understand how is centromere variation generated and especially, the consequences at a functional level. The specific objectives of my project are: (1) Identify the mutagenic processes and DNA repair responses operating at centromeres; (2) Determine the impact of centromeres sequence variation on chromatin structure, kinetochore function and chromosome behavior; and (3) Understand how these changes contribute to genome instability, cellular phenotypes and disease predisposition. The originality of this project is to follow a multidisciplinary approach that combines experimental studies  spanning structural biochemistry to cell biology  and bioinformatic analyses, that will benefit from the information on centromere reference already available and soon to be generated. The proposed research therefore represents a very appropriate and timely contribution to provide an integrated view of human centromere variation and its role in determining phenotypic traits. Furthermore, it will
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Suppression of Enzymes Required for Progression of LUng Cancer' under Seventh Framework.

assistant: **Concept**

Suppression of enzymes required for progression of lung cancer: we propose to combine an elegant genetically engineered mouse model (GEMM) of lung cancer with an in vivo RNAi screening approach in order to functionally identify a) biomarkers of early lung cancer development and b) candidate therapeutic targets whose activities are specifically required for the transition from benign to malignant disease.  We will use emerging RNA sequencing technology to identify changes in gene expression associate with early cancer progression in GEMMs of lung cancer and then select targets for RNAi suppression based on a stringent set of selection criteria aimed at zeroing in on potential drug targets, i.e.. proteins with enzymatic activity of any druggable nature that increase with tumour progression.  We will then generate focused libraries of shRNAs to suppress expression of selected targets and screen their efficacy in vivo using a cunning ""drop-out"" screening method.  We will then subject our best candidates to rigorous validation in order to assess their suitability as therapuetic targets in the context of human lung cancer.""