RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Search for the missing unicellular relatives of animals' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

How animals emerged from their unicellular ancestors remains a major evolutionary question. Work done on diverse unicellular relatives of animals demonstrated that the unicellular ancestor of animals had a larger repertoire of genes associated with multicellularity than previously thought. These include “animal-specific” genes such as protein tyrosine kinases, integrins and Brachyury. This suggests a latent genetic potential in place at the origin of animals and hints at a much more gradual transition in their emergence. However, a comparison of extant early-branching animals and their unicellular relatives still reveals an abrupt difference between protists and the body plans of extant animals. This gap could be due to intermediate lineages going extinct, or that descendants of key lineages have not been found yet. Recent DNA environmental surveys suggest the latter, revealing several novel kingdom-level lineages that branch close to animals and remain unknown. We will not make progress in understanding the origin of animals until we have explored the real diversity of animals’ closest relatives and isolated the major lineages that remain uncharacterized. Indeed, some of the answers to animal origins that we can not currently address with our taxon sampling are likely hiding in hindsight in those unsampled lineages. Notably, a targeted survey of animal relatives has not been done. I propose to do this by exploiting recent developments in long-read metabarcoding. We will screen different environments and isolate the novel lineages using fixation-free labeling methods. We will culture them and get their genomes.We will provide a complete picture of the diversity among animal relatives, which will also be relevant to ecologists. Notably, the novel lineages will allow us t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Role of platelet polymorphisms and membrane glycoproteins in clinical outcomes following percutaneous coronary interventions' will create measurable scientific, societal and economic impact aligned with Fifth Framework.

assistant: **Impact**

Rest enosis is a major problem following coronary angioplasty. Platelets may play a key role, therefore a better knowledge of their function in this process will give us a rationale to optimise the management of patients undergoing coronary angioplasty using newly developed platelet receptor inhibitors.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Sixth Framework proposal on 'LIFT - Laser-Induced Fluorescence Transient: a remote sensing approach to scale and quantify photosynthetic light use efficiency in ecosystems'.

assistant: **Innovation**

Determining the spatial and temporal diversity of photosynthetic processes of complex plant canopies presents many challenges, yet this information is required to improve carbon and climate models. The impacts of environmental stresses on the photosynthetic efficiency of leaves in the canopy have been included in current models but these parameterizations have not been properly validated. Some environmental stresses, such as low and high temperatures and excessive irradiance inhibit photosynthesis directly. These limitations can be quantified non-destructively by chlorophyll fluorescence measurements. With this project, we propose to further develop a new system for laser-based remote sensing (LIFT) of photosynthetic efficiency at a distance of 5 to 50m.This technique compares well with leaf level measurements, and was tested for scanning photosynthetic efficiency in spatially extended canopies producing a map of photosynthetic efficiency of individual leaves as a function of their local environment in the canopy. We will apply the LIFT to map the progress of reduced photosynthetic efficiency by photo-inhibition under cold stress and excess light. Mapping these transitions will help us to understand the complex interactions of damage and repair processes of the photosynthetic apparatus and its overall impact on canopy photosynthesis. An improved representation of these processes in photosynthesis models will lead to a better understanding of CO2 exchange in plant ecosystems exposed to environmental stresses and will help to improve confidence in carbon models. During the return phase we will use the expertise obtained during this project to establish a European centre of competence in remote sensing of photosynthesis.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'The role of mRNA-processing bodies in ageing'.

assistant: **Objectives**

Recently, we and others have revealed that, in the nematode Caenorhabditis elegans, reduction of protein synthesis rates in somatic cells extends lifespan. Based on this, we postulate that the molecular factors and mechanisms that control the mRNA metabolism in post-mitotic cells are critical determinants of ageing. This project will validate this hypothesis using C. elegans as main model system, but parallel studies in Saccharomyces cerevisiae and Drosophila melanogaster will prove the conservation of our observations. The cellular factors involved in mRNA metabolism (degradation/storage) are localized at specific particles in the cytoplasm of all eukaryotic cells, termed mRNA processing (P) bodies. Additionally, stress granules are cytoplasmic sites of mRNA-metabolism that are formed under stress conditions in mammalian cells. The objectives of this project include: -Monitoring of both P bodies and stress granules in adult worms and characterization of the age-related alterations in their profile, by immunostaining and real-time fluorescence imaging -Direct alterations in the expression of genes encoding factors of each particle in wild-type worms and analysis of the effects on lifespan and stress resistance -Comparison of the age-related changes in the profile of P bodies and stress granules between wild-type and long- or short-lived mutant worms -Direct alterations in the expression of genes encoding factors of each particle in worms with altered lifespan and investigation of the effects on lifespan and stress resistance -Observation of the age-related alterations in the profile of P bodies in yeast and flies, both in wild-type and long-lived strains. The rationale for this project is to provide insight into the modulation of ageing and stress resistance at the leve
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Meta-Antenna and Energy harvesting/storage modules development for autarkic sensors arrays' demonstrating excellence, impact and implementation quality for a Horizon Europe call.

assistant: **Abstract**

METATHERM will demonstrate a versatile energy harvesting and communication system for unattended environment sensors arrays, allowing the development of maintenance free and autarkic operation devices. The novelty builds up from several different innovations, the main one being the microwave antenna, based on epsilon engineering using metamaterials, allowing the antenna to show extremely high gain and an extreme compactness [FETOPEN Project NANOPOLY]. The energy efficiency of the antenna allows the electrical power need to be decreased accordingly, which allows the consideration of novel energy harvesting technologies to be used to power the system. In METATHERM, the energy of the sun is harvested using a novel ionic thermoelectric device. This technology is based on ion migration under a thermal gradient, and show higher Seebeck coefficient than usual tellurium based semiconductors without the use of rare or toxic elements: a significant advantage in the context of the rapidly growing energy demand for IoT and mobile electronic systems. Used in addition to a supercapacitor to store the electrical energy and a thermal energy storage device, METATHERM platform will allow continuous operation of the device day and night. Moreover, due to the similarity in device structure between the iTE device and the supercapacitor, METATHERM will benefit from manufacturing know how of the latter. A substantial maturity level increase is then expected for the iTE devices. Finally, the project aims to develop a platform integrating all these technology in a single device, and exploration of its potential applications. To ensure market fit, the project plans a 3 phases exploitation plan, gathering potential user interest at the beginning of the project, selecting most promising applicatio
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Measuring and modeling how misregulation in gene regulatory networks causes intellectual disability' to be submitted under Seventh Framework.

assistant: **Abstract**

Intellectual disability (ID) is a neurodevelopmental disorder with a strong genetic component. For many patients the genetic cause is yet unknown as no protein-damaging mutations can be identified. To accurately predict the risk for ID from a person’s complete DNA sequence, it is essential to know how and when genetic perturbation of a gene regulatory element causes abnormal neurodevelopment. The knowledge and statistical methodology to do so are currently lacking.Recently, the ENCODE and Roadmap Epigenomics projects identified millions of gene regulatory elements across a wide range of tissues. These regulatory elements are small DNA regions where cooperative binding of specific transcription factors (TFs) strongly influences the level of expression of one or more genes, often in a highly tissue-specific manner. However, since only static characterizations are available, it is very difficult to predict how the transcriptional network defined by the regulatory elements responds to genetic perturbations.I will therefore perturb gene regulatory elements underlying ID by knocking-down the expression of established ID transcription factors in neurons. Previous efforts relied on partial or noisy measurements of TF binding. Instead, I will use TF-footprinting to obtain a complete view of how the knock-down perturbs TF-binding in gene regulatory elements. Combining this with gene expression and epigenetic profiling, I will model of how perturbation of cooperative binding in regulatory elements causes misregulation of ID and neurodevelopmental genes. To predict how perturbation of the transcriptional network disrupts biological pathways, I will integrate in my Bayesian network model existing data sets of neuronal morphology, structural brain imaging GWAS, and behavioural studie
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'DryGro: Efficient Growth of Crops in Arid Environments (DEGCAE)' (DEGCAE), addressing dissemination and exploitation.

assistant: **Impact**

DryGro is an agriculture technology company that has developed an innovative new way to grow animal feed crops using very little water. This method uses large, 20m x 200m closed environment growing units to grow a high-protein animal feed crop called lemna, and can replace traditional animal feed staples like soy meal and sunflower cake which are very environmentally damaging. DryGro growing units have a number of advantages to traditional feed growing. Units are placed above ground, and thus avoid the need to compete with traditional growing methods for use of high quality arable land in the production process. Using this method, animal feed protein can be grown at a market price cheaper than is currently available on the market today. Current testing indicates that this method reduces water consumption per gram of protein by over 99%. DryGro technology increases yield per hectare by around 8x compared to traditional protein ingredients like soy. The market for feed produced by this technology is valued at over $150bn per year.  The key USPs of DryGro's technology are: 1) Ability to grow high-nutrient crops in areas otherwise unsuitable for agriculture; 2) 99% reduction in water consumption; 3) cost reduction of >50% per unit of output and 4) flexibility in choice of location, thus shortening supply chains and reducing costs. This feasibility study seeks to explore the technological and economic viability of expanding operations into three European target markets (Spain, Italy and Greece; selected as they face significant threats regarding aridity/desertification). It is hoped that the Phase 1 feasibility study will select the most suitable 1-2 markets of these countries, and underpin a Phase 2 application to enable DryGro to undertake trials in the target countries.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Structural characterization of YidC, a novel component of the membrane proteins translocation machinery' goes beyond current state-of-the-art under Sixth Framework.

assistant: **Innovation**

Understanding membrane protein biogenesis is of central importance for modern society as membrane proteins are not only crucial for transport or acting as receptors, they also comprise a major drug target for phamaceutical industry. In E. coli membrane protein biogenesis requires components of the well- described SRP and Sec pathways for targeting to and insertion into the membrane. However, a clear picture of the integration of membrane proteins into the lipid bilayer has not yet emerged. Recently, a membrane protein (YidC) essential for viability of E. coli has been identified. Members of the YidC family exist in all three domains of life where they have multiple functions in the integration and assembly of a large variety of membrane protein complexes.The host institution has established an efficient expression and purification protocol for YidC which allows now for the detailed structural and biochemical characterization of YidC alone as well as in complex with functionally relevant partners. Several detergents will be systematically screened for their influence on the oligomeric state of YidC as well as on its function. The interactions of YidC with the SRP system will be analysed and potential binding partners e.g. the SRP receptor, will be included in t he biochemical and structural studies. Besides E. coli, thermophilic bacteria will be used as source for YidC and also chimeras will be tested for structure analysis.The results obtained will not only contribute to the molecular details of membrane protein biogenesis, but will also add to a rational optimisation of membrane protein production by engineering the membrane insertion pathway. Due to the combination of biochemical and biophysical methods this project represents an excellent opportunity to strengthen my
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Strategies to prevent congenital toxoplasmosis: a systematic review of the evidence and its implications for practice and policy' under Fifth Framework.

assistant: **Concept**

The project will review the research evidence relating to prevention options for congenital toxoplasmosis and determine the implications for practice at a time when new research is becoming available. Systematic reviews including individual patient data analyses of cohort studies, will examine the effects of prenatal treatment on outcomes in children and explore whether these effects vary between settings or groups of patients. These reviews and in addition, syntheses of research relating to primary prevention, neonatal screening and the risk of long term impairment due to congenital toxoplasmosis, will be reviewed by an independent working group. A consensus conference of clinicians, patients, parasitologists and policy makers across Europe will determine the implications of the research findings for different health care settings. Finally, a working group will examine the need for co-ordinated research to evaluate future practice.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Identification of susceptibility genes in multiple sclerosis' goes beyond current state-of-the-art under Fourth Framework.

assistant: **Innovation**

To identify genes with an importance for susceptibility, resistance and disease course in multiple sclerosis, thereby increasing the understanding of the underlying pathogenetic events. The project consists of the following tasks:The identification, collection and clinical and physical characterization of a clinical MS material gathered from an ethnically very homogenous background.A genome-wide search of 390 polymorphic DNA markers spanning the entire genome in 100 affected sibling pairs. Confirmation of the importance of identified chromosomal segments will be sought in an additional 100 multicase families.Selected candidate genes, based on presumed pathogenetic mechanisms, will be studied directly in the sib-pairs and confirmed in the multicase families.Selected genes or chromosomal regions, previously implicated or identified within the project, will be investigated in a very large number (500-1000) of sporadic MS patients for the delineation of associated haplotypes.Parallel and subsequent analysis of an ethnically more distant reference population. This project offers a unique opportunity in this type of analysis due to the ethnic similarities between the Scandinavian populations rendering a, sufficiently large, homogeneous, study population, likely to yield a high power of resolution in the analysis of a polygenic disorder. At completion, the project is likely to have identified a number of genes or delineated chromosomal regions containing genes, with a role in pathogenetic mechanisms in MS.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Development of a rapid high-throughput assay for sensitive and specific detection and strain typing of creutzfeldt-jakob disease based on fluorescence correlation spectroscopy' (1998-2002) under Fifth Framework.

assistant: **Objectives**

Prison diseases such as Creutzfeldt-Jakob disease (CJD) and BSE have caused a major concern in regard to public health. We used a novel approach based on fluorescence correlation spectroscopy to develop a highly sensitive detection method for priors termed SIFT (Scanning for Intensely Fluorescent Targets), which has successfully been applied to CSF samples. Moreover, the labelling ratio of two independent probes can be analysed on a single particle level. By this approach, different types of prison particles associated with different variants of CJD could be distinguished in pilot experiments. Within the project, we want to develop this method into a rapid and sensitive diagnostic test that can be used on a routine basis for diagnosis and strain typing in CJD. In addition, we aim at harmonizing and validating biochemical typing of CJD variants at the European level.