RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Deciphering the mechanism of the dual functional E3 ligase TRIM28 in cancer cells' (2014-2020).

assistant: **Concept**

TRIM family proteins are RING-type E3 ubiquitin ligases with important roles in immune responses, autophagy and cellular signalling. They are characterised by the presence of an N-terminal tripartite motif that harbours the catalytic RING domain and a C-terminal variable substrate-binding domain. TRIM28/KAP1 is a unique family member that is better known for its role in transcriptional repression, which is mediated via its C-terminal PHD-Bromo domains. Nevertheless, ubiquitination activity has been described for TRIM28, especially in cancer cells, in conjunction with Melanoma Antigen (MAGE) proteins. MAGE proteins are aberrantly expressed in many cancer types and have been proposed to regulate and activate TRIM28 E3 activity to mediate proteasomal degradation of prominent targets, including p53 and AMPK. Cellular and molecular details of TRIM28 MAGE-dependent ligase activity remain unknown. Furthermore, it is unclear, how the interplay between the two functions of TRIM28 is regulated and how the protein E3 ligase activity is supressed when recruited as part of transcriptional silencing complexes. I want to use a multi-disciplinary approach and combine unbiased proteomic studies with in vitro biochemical, biophysical and structural experiments to study MAGE-dependent regulation of TRIM28 E3 ligase activity. I aim to identify cellular signals such as posttranslational modifications and interaction partners that can both modulate MAGE-TRIM28 complexes and regulate the interplay between the transcriptional and the E3 ligase activity. By combining these findings with structural and mechanistic studies, I will uncover how MAGE proteins modulate substrate recognition, E2 recruitment and ligase activity of TRIM28, and create a model of MAGE-TRIM28 function.


**Keywords:** ubiq
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Developing the iTOP kit: Empowering scientists with efficient intracellular delivery of biologicals in hard-to-manipulate cell types' to be submitted under Horizon 2020.

assistant: **Abstract**

NTrans Technologies BV has developed iTOP; a novel and extremely effective method to deliver highly relevant bioactive molecules (including DNA, RNA, active proteins, and their combinations) into mammalian cells. This includes the efficient delivery of such molecules inside the most biologically and biomedically relevant cell types (e.g. human primary and stem cells), which are notoriously difficult to manipulate. iTOP technology has been scientifically validated (published in the top scientific journal Cell in 2015) and patented (WO2015028969).iTOP addresses the unmet need of researchers and drug developers to be able to transfect a range of biomolecules into biologically relevant cell models; in particular to study genetic diseases and to underpin results obtained with standard immortalized cell lines.The ENTRANCE project includes the necessary steps towards market entry of the iTOP research-use-only (RUO) kit: The development of (i) an iTOP RUO kit and (ii) an iTOP on-line community as innovative marketing tool that will support proper use and stimulate efficient application of the technology. The ENTRANCE project will thus provide the scientific community access to the novel iTOP tool, facilitate direct access of iTOP kit users to the NTrans support team, and enable sharing of user experiences via an iTOP interactive forum.ENTRANCE will instigate a revolution in biomedical sciences by empowering both researchers and drug developers to manipulate the most relevant cell types with unprecedented efficiency. This will allow the field to move forward at a significantly accelerated pace.The iTOP RUO kit will generously earn back the investments within 4 years, and help to create a buzz around the merits and possibilities of the technology. The latter is of immense value t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'European airport Movement Management by A-smgcs', explaining its relevance to EU policy priorities under Sixth Framework.

assistant: **Impact**

Mobility of goods and people is a basic need of the European Society in the age of sustainable growth and globalisation of the World economy. The Vision 2020 describes Air Transport and Aeronautics as 'Key Assets for the Future of Europe' and quantifies ambitious goals to meet society's needs and to win global leadership. It calls for an air transport system able to cope with a tripled air traffic demand with fivefold safety, halved cost, a 99% punctuality and limited environmental impact in 17 years from now.The European way to reach these goals is outlined by the EUROCONTROL EATMP Programme (i.e. Airport Operations) and ATM2000+ Strategy as well as the ACARE SRA work. They identify airports as the future bottlenecks of air transport and see mid-term solutions in: '...enhanced operational concepts, supported by new decision-making or decision-support tools to ensure more efficient use of the airport infrastructure...'. They say 'A-SMGCS are seen as key R&D topics' (ACARE). Holistic approaches like A-SMGCS
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'NAnomechanical Screening of Pharmaceutical Entities' (NASPE).

assistant: **Innovation**

Microcantilever (MC) biosensors are innovative and versatile micro-electro-mechanical systems (MEMS). Due to (nano)mechanical transduction of biochemical reactions they allow sensitive, label-free, direct, real-time and multiplexed detection of molecules as well as specific investigations of molecular interactions and conformational changes.  NASPE aims at generating the pre-requisites and supporting the effort to establish MC biosensors as a competitive technology in the field of drug screening by integrating skills and know-how of SMEs and RTD performers which cover leading positions in the fields featured by the project.  NASPE outcomes are expected to enable the SMEs to strengthen their position in the international market of drug discovery, to gain access to new market segments and to create new highly skilled jobs in Europe. On the other hand, NASPE research activities will contribute to address inherent fundamental issues of MEMS biosensors, including functionalization, manufacturing and novel applications. The primary aim of NASPE is structured into the following SMART objectives: - Functionalization protocols for silicon and plastic MCs, necessary for performing molecular recognition experiments by MCs. They will be accomplished by months 9 and 18, respectively, and tested on conventional fluorescent essays. - Proof-of-concept of nanomechanical drug screening by MCs, that is the necessary step for designing MC essays. Experiments for silicon and plastic MCs will be assessed and statistically validated by month 12 and month 21, respectively. - Prototype plastic MC arrays, that will be provided by month 15 and tested against commercial silicon MCs. Plastic MCs are expected to have lower cost and enhanced performances with respect to silicon MCs. - “Field” test of
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Advanced ultrafast electron sources for quantum optics experiment with free electrons' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

This proposal aims at the development of an electron source that will allow controlling the position of free electrons with extremely high precision in space and time. Such a tool will enable novel quantum optics experiments and might turn into an electron source widely used in time-resolved scanning electron microscopy and electron diffraction, a field in which Europe is currently trailing the US. The proposed experiment will be a complementary effort to the applicant’s research that he is currently setting up with a Max Planck Junior Research Group Grant. After his PhD work in T.W. Hänsch’s group the applicant spent four years as postdoctoral researcher at Stanford University and returned to Europe in November 2007. The proposed experiment will transfer much of the applicant’s experience from the US and is based on the combination of a special field emission tip he developed in the US and a femtosecond laser oscillator. Femtosecond laser pulses hit the tip and so emit electrons from nanometric dimensions within timescales of a femtosecond. The final goal is a deterministic electron source, where one and only one electron is emitted with every laser pulse. Such a source would immediately enable classic quantum optics experiments. Advanced emitters will be used that exhibit quantum behaviour such as single atom tips and carbon nanotubes where emission takes place from a single or few atoms only. The proposed work would ideally augment the experiments that the applicant will perform with the Max Planck funds and will initially go in parallel to these. Proposed experiments will potentially allow highly increased visibility of his research and will help him to take the next step towards a faculty position at a respected research institution in Europe. Furthermore, expected
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Seventh Framework proposal on 'Impact of temperature on the vulnerability of Mediterranean seagrasses to phatogens'.

assistant: **Concept**

The increasing rate of global warming predicted for the coming decades will significantly impact coastal ecosystems. Habitats of particular concern in light of the predicted temperature increase are seagrass meadows, which are sensitive indicators of coastal and estuarine health. Another potential threat to seagrasses are pathogens. Pathogens are present in seagrasses around the world and have been responsible for large seagrass losses in the past. In the Mediterranean, the presence of pathogenic organisms in seagrass tissue has not yet been shown to increase seagrass mortality, but it is thought that pathogens pose a significant risk of seagrass decline under deteriorating environmental conditions. While recent efforts have been made to examine the effects of increasing temperature on seagrasses, the occurrence of pathogens is not well studied, and we know little of what combined effects these stressors may produce. In this proposal, I use seagrasses, a recognized sensitive indicator of environmental change, to develop a first effort at evaluation of future global scale changes that result from interactions of temperature and a pathogenic protist Labyrinthula sp. The hypothesis to be tested in this project is that increased temperature makes seagrasses more vulnerable to pathogens and that the combined effects of pathogenic infection and warming would be additive and cause seagrass mortality at lower temperatures than is observed for healthy seagrasses. I will carry out a field study and a series of laboratory experiments to examine the interactive effects of warming and pathogens.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Factors Affecting Training Transfer' to be submitted under Seventh Framework.

assistant: **Abstract**

Training transfer is defined as the extent to which trainees apply their knowledge, skills and attitudes acquired in training to the working situation. Training transfer has been discussed in a number of different of fields of human behavior including psychology, anthropology and economics. Generally, there are three groups of factors influencing training transfer. These factors represent aspects of trainee characteristics, training design, and work environment. However it has been argued that our understanding of real transfer issues is still incomplete. The question of how researchers with innovative ideas should proceed from this state is still open. Training transfer can not be successful if the trainee has no intention, has been called (training) transfer strategy, to transfer learned skills and knowledge to his/her workplace. Therefore, this project will explore the role of transfer strategy and its interaction with training design, trainee characteristics and work environment as these affect training transfer. The project has the potential to create a new framework for the development of a systematic approach of the transfer process. This is a novel approach never attempted in the existing literature. To our knowledge, none of the models that exist today explain the effect of combination of training design, trainee characteristics and work environment, with transfer strategy influence to explain and predict training transfer. The project provides an important further step toward understanding training transfer by taking into account the effects of other transfer-influencing factors. This research results are not only is directed to the academic community, but also to other possible beneficiaries such as policy makers or business leaders. It gives importance to th
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Additive Micro-Manufacturing for Plastic Micro-flectro-Mechanical-Systems' under Horizon 2020.

assistant: **Concept**

The manufacturing of silicon-based MEMS today is well advanced because the micro-electro-mechanical devices for automotive, domestic, health-care and consumer electronics can be fabricated with methods from IC industry. Polymer-based MEMS have a great potential for flexible electronics and biomedical applications, but to date, the techniques to engineer functional polymers into 3D microsystems, are still at their beginning because a coherent fabrication platform with the right tools and processes does not yet exist. The field could tremendously benefit from a coordinated effort in materials and manufacturing, in particular with a focus on biocompatible plastic materials for biomedical applications. Additive manufacturing such as 3D printing and associated processing such as sintering has already started to transform traditional industry, but is not scalable much below a micrometer because the thermal processing is done in bulk or by lasers on surfaces. MEMS 4.0, in analogy with the industry 4.0 concept, aims to perform concerted research in additive manufacturing at the micro/nanoscale and associated key techniques. Using my expertise in MEMS and Nanotechnology, MEMS 4.0 will push the frontiers in new materials and new processing for MEMS by setting a focus on stencilling, printing, self-assembly and local thermal processing. This coherent processing framework will permit the use of delicate, soft, polymer materials to engineer the next generations of plastic MEMS. We are primarily targeting biodegradable implantable MEMS and permanently implantable glassy carbon MEMS. They are the most challenging to fabricate, but if successful, they also have an enormous impact for future wearables and implantables.


**Keywords:** Inkjet printing, nanostenciling, thermal nano-proces
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'New methodology for rubber and cork recycling to produce footwear insocks', explaining its relevance to EU policy priorities under Fourth Framework.

assistant: **Impact**

In EU, rubber industry for footwear applications produces annually about 1,500,000 tons of solid wastes which are mainly disposed underground. Since this solutioni) implies wasting all the contained resources,ii) has associated high management and land occupation costs,iii) usually raises the opposition of the nearby populations, represents a serious environmental and economic problem for the rubber and footwear industries. Cork industry is very important specially in the South of Europe. Portugal alone produces more than 50% of the world's cork production, which is used to produce bottle stoppers and compositions of cork. The production of these originates great quantities of solid wastes, in the form of high and low diameter particles. The first are actually completely reused. However for the second, normally refereed as dust, no high value application has been identified, and nowadays are only used in the cork factories as combustible for boilers, being very excedentaries. The goal of this research project is to contribute to the competitiveness of these industries by achieving the following industrial objectives: - Development of a new rubber/cork material for footwear insocks, incorporating at least 30% of these wastes; - Create a sustainable development alternative by economical and environmental correct management of the referred residues; - Introducing a new process for insocks design and production taking in consideration comfort parameters, and obtaining the patent for subsequent commercialisation; - Contributing to the overall increase of comfort in footwear which is the major decision factor for consumers selecting a new pair of shoes, in Europe, giving European footwear and related industries an additional competitive factor.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Autopoietic Cognitive Edge-cloud Services' (ACES).

assistant: **Innovation**

The increasing need for cloud services at the edge (edge–services) is caused by the rapidly growing quantity and capabilities of connected and interacting edge devices exchanging vast amounts of data. This poses different challenges to cloud computing architectures at the edge, such as i) ability to provide end-to-end transaction resiliency of applications broken down in distributions of microservices; ii) creating reliability and stability of automation in cloud management under increasing complexity iii) secure and timely handling of the increasing and latency sensitive flow (east-west) of sensitive data and applications; iv)need for explainable AI and transparency of the increasing automation in edge-services platform by operators, software developers and end-users. ACES will solve these challenges by infused autopoiesis and cognition on different levels of cloud management to empower with AI different functionalities such as: workload placement, service and resource management, data and policy management. ACES key outcomes will be: i) autopoiesis cognitive cloud-edge framework; ii) awareness tools, AI/ML agents for workload placement, service and resource management, data and policy management, telemetry and monitoring; iii) agents safeguarding stability in situations of extreme load and complexity; iv) swarm technology-based methodology and implementation for orchestration of resources in the edge; v) edge-wide workload placement and optimization service; vi) an app store for classification, storage, sharing and rating of AI models used in ACES.ACES will be demonstrated and validated in 3 scenarios demanding for support of highly decentralised computing, ability to take autonomic decisions, reducing costs of cloud-edge management and increasing their efficiency ,th