RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Structure and Function of the Bacterial Transformasome', explaining its relevance to EU policy priorities under Horizon 2020.

assistant: **Impact**

Natural genetic transformation, first discovered in Streptococcus pneumoniae by Griffith in 1928, is observed in many Gram-negative and Gram-positive bacteria. This process promotes genome plasticity and adaptability. In particular, it enables many human pathogens such as Streptococcus pneumoniae, Staphylococcus aureus or Neisseria gonorrhoeae to acquire resistance to antibiotics and/or to escape vaccines. For natural transformation to occur, bacteria must develop a highly regulated physiological state called competence, during which a multiprotein machinery, designated as the transformasome, is specifically expressed. The transformasome drives the uptake of extracellular DNA and its subsequent integration into the bacterial genome. This intricate system can be divided into three functional entities: the transformation pilus, the DNA entry pore and the recombination apparatus. The architecture and function of the transformasome remains largely elusive. We propose to dissect the structure and function of this system in the human pathogen Streptococcus pneumoniae. We will combine four complementary approaches to attain our goal: (1) Molecular biology and biochemical studies in vitro (2) Structural biology on purified proteins or protein complexes using X-ray crystallography and cryo-electron microscopy (3) Structure-function studies in vitro and in vivo (4) Structural biology in situ using cryo-tomography.Overall, we believe this ambitious project will provide major insights to understand the molecular basis of bacterial transformation, a fundamental process in bacterial physiology. In addition, this project may provide new strategies to reduce pneumococcal adaptation during infection.


**Keywords:** Membrane protein complexes
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Novel genetic engineering approaches for lineage analysis and exploration of Akt function in cortical development' (BRAINBOWAKT).

assistant: **Innovation**

The mammalian cortex, site of our cognitive functions, is a complex three dimensional environment which develops through an elaborated spatiotemporal choreography. A slight imbalance in this process can lead to devastating pathologies such as autistic spectrum disorders. While tremendous progress has been made in understanding early cortical development, several questions remain unanswered: how do minicolumns, the basic functional units of the cortex, establish their complex yet highly stereotyped organization, and what signaling pathways regulate this developmental process. In this proposal, I will use novel transgenic strategies and imaging techniques to address the following aims: 1) to characterize the ontogenesis of functional minicolumns, and 2) to investigate how activity of a key integrator of intracellular signaling pathways, Akt kinase, may influence both cortical cell commitment, migration and final positioning and the subsequent formation of functional minicolumns. To explore those questions, I will use the Brainbow technique, recently developed to drive combinatorial gene expression in a cellular population. First, this strategy will allow me to mark multiple adjacent cortical precursors with distinct labels, and thus track their progeny during minicolumn ontogenesis. Second, I will use the Brainbow approach to study Akt function by modulating its activity in a mosaic manner. To this aim, I will generate a construct able to stochastically express distinct Akt variants, showing either a constitutive action or dominant negative effect. This will allow me to investigate for the first time and in an intact developing brain how variations in the activity level of a kinase protein may differentially impact the lineage of neighboring neural precursors. This work w
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon Europe proposal on 'building vascular networks and Blood-Brain-Barriers through a Biomimetic manufacturing Technology for the fabrication of Human tissues and ORgans'.

assistant: **Innovation**

Tenths of millions of people with organ failures or suffering from degenerative diseases are waiting for a novel cellular therapy or for a transplant of a donor compatible organ and the immense majority of these patients will die before receiving the tissue or organ they need. Despite the significant advances in tissue engineering, not a single artificial tissue has been used to replace a part of an organ, with the exception of simple or avascular tissues like skin or cartilage. THOR proposes the development of a revolutionary tissue engineering technology, capable to produce any type of human tissue for organ repair or replacement in case of illness, trauma or degenerative diseases. Patient-tailored tissues will be fabricated by pools of bioinspired mini-robots in fully automated production plants, using the breakthrough incremental technologies of the THOR project: self-assembling molecules inspired to the extracellular matrix; self-assembling solid and hollow polymeric fibers; materials functionalization using photoactivable crosslinkers; cutting-edge mini robots to wave 3D self-assembling structures with factors and cells with micrometric precision. THOR tissue arises from high-resolution 3D spatial positioning of self-assembling structures, angiogenic factors and relevant cell lineages, reprogrammed and expanded in a dedicated bioreactor under controlled conditions. Our long-term vision encompasses (1) the establishment of a Tissue Engineering industry for personalized organ repair, producing any type of well vascularized and fully functioning tissues and (2) the maintenance of such tissues alive for long periods of time, for an easy transportation to hospitals and clinics, even far from the big cities. THOR will foster the rise of a new industry and of a new biome
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Architectures for Mobility' (AGILE) under Fifth Framework.

assistant: **Objectives**

Architecture-based approaches have been promoted as a means of controlling the complexity of system construction and evolution, namely for providing systems with the agility required to operate in turbulent environments and adapt very quickly to changes in the enterprise world. Recent technological advances in communication and distribution have made mobility an additional factor of complexity, one for which current architectural concepts and techniques are not prepared for. AGILE will provide means for addressing this new level of complexity by developing an architectural approach in which mobility aspects can be modelled explicitly and mapped on the distribution and communication topology made available at physical levels. The whole approach will be developed over a uniform mathematical framework based on graph-oriented techniques that will support sound methodological principles, formal analysis, and refinement.Architecture-based approaches have been promoted as a means of controlling the complexity of system construction and evolution, namely for providing systems with the agility required to operate in turbulent environments and adapt very quickly to changes in the enterprise world. Recent technological advances in communication and distribution have made mobility an additional factor of complexity, one for which current architectural concepts and techniques are not prepared for. AGILE will provide means for addressing this new level of complexity by developing an architectural approach in which mobility aspects can be modelled explicitly and mapped on the distribution and communication topology made available at physical levels. The whole approach will be developed over a uniform mathematical framework based on graph-oriented techniques that will support sound met
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Modelica Electrical System Simulation and Analysis' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

This proposal aims at improving Modelica and the V6 Systems solutions related to Dymola technology, Dymola V6, to better address electrical modeling and simulation. The tool improvements will be developed by Dassault Systemes and the Austrian Institute of Technology will contribute by evaluating the new features and by providing new Modelica libraries.The new features that will be developed include multi-core simulation support, interactive parameter studies, improved post-processing and automatic documentation, requirement modeling, and model layers in the graphical user interface. Many of these improvements could be extended also outside of the electrical domain and would make Modelica and Dymola V6 even more attractive for industrial, large-scale, model-based development.""
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Zinc and iron homeostasis in Arabidopsis thaliana: novel molecular factors and their influence on metal speciation and localization', explaining its relevance to EU policy priorities under Seventh Framework.

assistant: **Impact**

The central objective of this project is to advance the understanding of the metal homeostasis network in plants. More specifically, the planned research is directed at a molecular physiological understanding of metal transport, metal partitioning and upstream regulatory pathways, which are required when iron availability is low or when excess zinc enters the plant. To gain insights of a novel quality, this project will combine molecular genetics, molecular biology and molecular physiology approaches with the implementation and utilization of cutting-edge imaging techniques newly developed in physics, which have not been available to the European molecular plant research community to date. The genetic model plant Arabidopsis thaliana will serve as the experimental system, enabling the fellow to take advantage of the ease of obtaining mutant genotypes and transgenic plants, short generation time and the small size of the plant, thus making the project feasible within its duration. The new knowledge obtained can be applied in crop improvement for bio-fortification and nutrient efficiency, as well as in restricting the accumulation of harmful heavy metals in edible parts of crop plants or, conversely, in the development of plants suitable for plant-based clean-up of soils, through molecular marker-assisted plant breeding. The  project will build on the profound expertise of the candidate in synchrotron—based imaging of plant tissues by targeting single-gene functions through comparative imaging of specific tissues from selected genotypes grown under carefully chosen metal regimes. This will involve in-depth training of the candidate in molecular biology and Arabidopsis molecular genetics in the context of the plant metal homeostasis network, the area of specialization of t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'CROSS-SECTORAL ALLIANCE AS THE KEY FOR INNOVATION-DRIVEN BUSINESS SUCCESS OF ESTONIAN AND GREEK REPRODUCTIVE HEALTHCARE' (NESTOR) under Horizon Europe.

assistant: **Objectives**

Constantly decreasing infertility rates and low success rates of in vitro fertilization (IVF) associated with the advanced age of couples create challenges in reproductive medicine. It is crucial to fill the existing gaps to unlock the socioeconomic benefits and boost the commercial viability of reproductive medicine in Europe. The gap in access to excellence is especially witnessed in Widening countries such as Greece and Estonia, where the academic sector is lagging behind, producing a low volume of commercialised innovations and low-quality clinical services. It poses obstacles in creating competitive positions for highly talented specialists with career perspectives and a strong interest in boosting local reproductive medicine, stimulating the brain drain, and minimizing life-long learning opportunities. The contribution of the NESTOR project to solving these challenges is aimed at improving the research and innovation performance of institutions involved in reproductive medicine by boosting the collaboration between industry and academia in Widening and non-Widening countries through cross-sectoral and international mobilities. The NESTOR consortium consists of 8 partners from academic and industry partners from the Netherlands, Greece, and Estonia, ensuring the knowledge transfer and advancement in four key reproductive medicine modalities for the reproductively aged population: personalised medicine solutions, IVF embryo and uterine health studies, and novel non-invasive prenatal testing technologies.In total, NESTOR will engage 23 ERA Talents from the Widening Countries, boosting their research skills, nurturing their innovative and entrepreneurial mindset, and providing them with other transferable skills. The well-tailored secondment methodology is aimed at ra
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon 2020 proposal on 'Personalized, bio-engineered skin grafts for the permanent treatment of skin defects.'.

assistant: **Innovation**

Every year in the world, more than 50M patients suffer from skin defects (e.g. burns, excision, etc.) and need surgical intervention to restore skin function. Standard of care, skin autografting, very often leaves these patients with scars. Scars are permanent and disfiguring, and can also be painful and highly debilitating. Indeed, they may also impair movement and growth, and require several follow-up surgeries, intense homecare and psychosocial rehabilitation. Hence, scars significantly affect patient’s quality of life, along with the economic burden for the health-care system. Furthermore, in the case of large wounds, donor site shortage represents a real clinical issue. denovoSkin™ is a patented, personalized, bio-engineered human skin graft classified as Advanced Therapy Medical Product (ATMP). It is engineered in large quantities starting-off from a stamp-sized skin biopsy of the patient, thus solving the donor site shortage issue. Moreover, because of its dermo-epidermal structure, denovoSkin is expected to minimally scar after transplantation. Its outstanding performance could considerably reduce or even avoids further corrective interventions, allowing for considerable savings in terms of costs. denovoSkin™ is granted with Orphan Drug Designation for the treatment of burns by Swissmedic, EMA and FDA. So far, more than €14M have been invested over the last 16 years of research into the development and the clinical testing of denovoSkin™. To have denovoSkin™ fully projected at a European and global level we need a further investment of €3.57M to set up a solid EU value chain able to meet the large demand expected during the commercialisation via developing the automation of manufacture. The proposed work in Phase 1 of the SME instrument fits into our overall pla
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Professional peer Environment beyond edge computing' (POPEYE) under Sixth Framework.

assistant: **Objectives**

Next generation collaborative systems will offer the mobile user seamless and natural collaboration amongst a diversity of agents within distributed, knowledge-rich and virtualised working environment. However, this ambitious goal faces numerous challenges from the underlying communication infrastructure through to the high level application services. These challenges, depending on the operational need, can be addressed in both technological and scientific terms.The POPEYE project focuses on supporting dynamic spontaneous collaborative group working environments with autonomous coordination and knowledge management support.When most of the currently available tools supporting collaboration exploit a rigid client-server architecture and rely on a communication infrastructure like the Internet, POPEYE's ambition is to get collaborative working free from such constraints.In this setting, the project will consider P2P over wireless ad hoc groups, where fixed infrastructure is not a prerequisite, where virtual communities can emerge spontaneously and share data with the appropriate quality of service (persistence, synchronisation, security).The POPEYE project willa) draw out an integrated overlay networking architecture that combines the stability and performance of infrastructure networks (when available) with the flexibility and spontaneous character of mobile ad hoc communications,b) develop a communication platform to provide efficient P2P management and communication primitives,c) develop higher-level context-aware, secure and personalised core services to facilitate application development by allowing the combination of user preferences with ambience information, such as time, location, user activity, and peers' presence.The POPEYE infrastructure will be assessed throu
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon Europe proposal for the project 'PRocess Innovations for the Mineral industry focusing on ROasting and Calcination Kiln technologies and supported by novel pre- and post-processing'.

assistant: **Objectives**

PRIM-ROCK addresses process efficiency through a multifaceted strategy addressing industrial processes from input to output, supported by an ambitious digital framework and transversal supporting activities. In particular, advanced techniques for the pre-processing of the raw material will be deployed to enhance its efficiency according to the requirements of the process, supplemented by simulations and decision support systems to increase the operational limits of flexibility in the feedstock domain. Additionally, the process intensification component of the project will design, develop, and validate innovative and efficient processes, as well as optimizing the existing ones. The aim is to develop a portfolio of technologies to a higher resource efficiency and lower level of GHG emissions of extractive industries. The project will focus on improving the resource efficiency of the calcination and roasting processes, that are commonly used in the mineral and cement industries.To this end, process simulations in collaboration with AI data-driven models will be utilized and a digital twin for each process will be developed. Finally, waste reduction and re-utilization strategies will be investigated targeting to the real-time processes’ adjustment capabilities to feedstock changes and tighter processing control solutions. All these components will be supplemented by transversal supporting activities, such as technoeconomic assessment, LCA, standardization etc. The PRIM-ROCK solutions will be demonstrated in 3 different ASPIRE sectors, namely Minerals (magnesite, laterite), Cement (limestone) and Non-ferrous metals (sphalerite, chalcopyrite). The consortium is composed of 7 SMEs along with 6 RTDs, 3 universities and 5 large enterprises, working together to achieve over 30% r