RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Functional Exploration of Biosynthetic Dark Matter in the Human Gut Microbiome' goes beyond current state-of-the-art under Horizon 2020.

assistant: **Innovation**

The human gut is the habitat for trillions of microbial cells living in synergy with each other and with the host. While metagenomic studies of the gut microbiome have provided a wealth of DNA sequence data, functional studies of gut bacteria remain challenging. To study the gut microbiome from a functional perspective, understanding host-microbe and microbe-microbe interactions is key. These contacts are often established by specialized molecules termed secondary metabolites or natural products (NPs). The machinery required to synthesize these metabolites is encoded in bacterial genomes by biosynthetic gene clusters (BCGs). Remarkably, even though 14,000 BGCs were identified in the human microbiota, very little is known about the identities and functions of their products. To address that, we will focus on NPs of the ribosomally synthesized and post-translationally modified peptide (RiPP) family, the second most abundant NP class in the human gut. The Piel group recently discovered unprecedented RiPPs with altered peptide backbones, challenging the paradigm that ribosomal synthesis is limited to the L-alpha-amino acid topology. These modifications include the excision of a tyramine moiety from a tyrosine-glycine motif, which introduces an alpha-keto-beta-amino acid in the peptide precursor backbone; and the epimerization of amino acids from L- to D-configuration. Enzymes homologous to these backbone-modifying catalysts and associated with RiPP gene clusters have been found bioinformatically in a wide variety of bacterial genomes, including gut microbiome representatives. The pervasiveness of these BGCs in microbiome bacteria suggests a function for these metabolites and possible therapeutic applications. This proposal aims to identify the products of these gene cluster
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Fucoxanthin Upregulation through Combined Omics and Metabolically Improving Control Segments' demonstrating excellence, impact and implementation quality for a Horizon 2020 call.

assistant: **Abstract**

The growing awareness towards health and disease prevention holds a great future globally for naturally derived nutraceuticals. This has led to a dramatic increase in demands of naturally synthesized carotenoids, particularly fucoxanthin.This golden-brown carotenoid is used as anti-oxidant, anti-cancer, anti-diabetic, and anti-obesity component making it a valuable product for nutraceutical and food industries. Diatoms, particularly Phaeodactylum, naturally synthesize fucoxanthin in high amounts as compared to their counterparts (seaweeds), making them one of the most promising candidates for sustainable fucoxanthin production. However, to exploit these diatoms for commercial fucoxanthin production, highly productive strains capable of growing at low operational costs along with a detailed understanding of the fucoxanthin biosynthesis pathway to steer conditions towards most favourable for high yields are imperative. To this end, FUCOMICS will develop an integrated omics pipeline (transcriptomics and proteomics) to gain insight into fucoxanthin synthesis and identify key regulatory elements/genes for enhancing fucoxanthin synthesis in the local Norwegian isolate Phaeodactylum tricornutum B58. Further, by utilizing Response Surface Methodology culture conditions will be optimized for maximum fucoxanthin synthesis. The identified key regulatory elements/genes will be validated by generating engineered strains of P.tricornutum B58 using the advanced CRISPR/Cas 9 technique. The results generated by FUCOMICS will not only be a starting step towards commercialization of microalgal fucoxanthin, but can also act as foundation for more difficult components, such as omega-3 fatty acids and biomass as whole. Furthermore, the fucoxanthin-extracted biomass, still rich in omega-3 fat
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Scalable End-user Access to Big Data' (Optique).

assistant: **Concept**

Scalable end-user access to Big Data is critical for effective data analysis and value creation. Optique will bring about a paradigm shift for data access by<br/>* providing a semantic end-to-end connection between users and data sources* enabling users to rapidly formulate intuitive queries using familiar vocabularies and conceptualisations* seamlessly integrating data spread across multiple distributed data sources, including streaming sources* exploiting massive parallelism for scalability far beyond traditional RDBMSs<br/>and thus reducing the turnaround time for information requests to minutes rather than days.<br/>These objectives will be achieved by bringing together leading experts and cutting edge technology from diverse communities to develop an extensible platform that will provide a complete and generic solution to the data access challenges posed by Big Data.<br/>The platform will: (i) Use an ontology and declarative mappings to capture user conceptualisations and to transform user queries into complete, correct and highly optimised queries over the data sources; (ii) Integrate distributed heterogeneous sources, including streams; (iii) Exploit massively parallel technologies and holistic optimisations to maximise performance; (iv) Include tools to support query formulation and ontology and mapping management; (v) Use semi-automatic bootstrapping of ontologies and mappings and query driven ontology construction to minimise installation overhead.<br/>Development of the platform will be informed by and continuously evaluated against the requirements of complex real-world challenges, with two large European companies providing the project with comprehensive use cases, and access to user groups and TB scale data sets.<br/>Experience from the use case deployment
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Fifth Framework proposal on 'Molecular mechanisms controlling chemokine receptor mrna stability'.

assistant: **Innovation**

mRNA stability is a major checkpoint for the expression of certain chemokine receptors, affecting leukocyte recruitment in sites of infection, tumours and inflammatory diseases. The project aim is to investigate how CCR2mRNA half-life is regulated and how this influence CCR2 gene expression. mRNA stability is a major checkpoint for the expression of certain chemokine receptors, affecting leukocyte recruitment in sites of infection, tumours and inflammatory diseases. The project aim is to investigate how CCR2mRNA half-life is regulated and how this influence CCR2 gene expression.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Improving COLOrectal cancer screening: Novel INverse and FOrward algorithms for a new real-time microwave endoscopy' (COLONINFO).

assistant: **Concept**

More than 470,000 European citizens are diagnosed every year with colorectal cancer (CRC), accounting 12.2% of the total number of cancer deaths. The cumulative risk of CRC in persons aged under 75 is 4.17% in men and 2.3% in women. Screening options are used in EU for the early detection of CRC and the most likely one (after positive faecal test) is the endoscopy. However, the reduced field of vision of the endoscopic camera (<180°), the non-homogenous illumination, the polyp concealment due to angulations and folds of the colon or poor colon cleansing, and the dependence on the experience of the clinician who performs endoscopy, result in lack of efficacy of this technique. It is estimated that after a negative colonoscopy the risk of having CRC is 60-70%.The host has just invented MiWEndo, a low cost system of microwave image generation for clinical endoscopic applications. The pre-prototyped device is a small endoscope head composed by an array of antennas that transmits and receives microwave signals, and it will form anatomical and functional images of the interior of the gastro-intestinal tract as the endoscope travels along it. The goal is to provide real-time (360°) images in endoscopic explorations and interventions, offer a better understanding of the CRC behavior (by allowing quantification), and guide diagnosis and therapy. The objective of this MSCA is to ensure the computational efficiency and robustness (in terms of resolution and computational time) by developing a reconstruction software able to guide diagnostic and allowing quantification in real-time (not yet possible). This project will be completed in a leading multi-disciplinary research group. The applicant brings mathematical and bioengineering skills that will facilitate research in the group a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Manufacturing of Integral Stiffened Skin Panels', explaining its relevance to EU policy priorities under Horizon 2020.

assistant: **Impact**

MISSP (Manufacturing of Integral Stiffened Skin Panels) project aims at proposing new manufacturing concepts for metallic aircraft cargo doors. The main objective of this project will be to develop cheaper and greener structures as an alternative to CFRP doors but also applicable for any stiffened skin panel structure. This will be made possible by the use of advanced materials, manufacturing techniques and design concepts. Expensive and polluting chemical milling operations shall be replaced by flat mechanical machining, while multi-parts riveted components shall be replaced by more integrated structures reducing the number of parts and the time-consuming mechanical riveting assembly processesThe project will be divided into 3 main technical work packages, followed by a cost and performance analysis. The first work package shall focus on the manufacturing of the external door skin with the aim of replacing the chemically milled pockets by mechanically machined ones. Both stretch-forming on tailored tool blocks and high energy hydroforming alternative will be investigated. In the second work package, stringers shall be integrated to the Al-Mg-Sc 5028 skin by flat welding (FSW or LBW) followed by creep forming. Finally, the third package shall be dedicated to the manufacturing of an integral structure, drastically limiting the number of assembly operations. To this aim, an innovative solution is proposed: a plate shall be deformed by high energy hydroforming and subsequently machined into the final cargo door integral structure.To support the advanced manufacturing technologies proposed in this challenging project, use shall be made of numerical modelling that will help designing appropriated tools and optimizing process parameters to achieve high quality demonstrators t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'ENDOTHELIN-3/ENDOTHELIN RECEPTOR B SIGNALING IN THE REGULATION OF NEURAL CREST STEM CELLS MIGRATION: THERAPEUTIC POTENTIAL FOR HIRSCHSPRUNG S DISEASE' to be submitted under Sixth Framework.

assistant: **Abstract**

Complete colonization of the gut by enteric neural precursors depends on activation of endothelin receptor B (EDNRB) by its ligand endothelin 3 (EDN3). Loss of EDN3/EDNRB signaling leads to aganglionosis of distal gut (Hirschsprung s disease, HSCR) caused by failure of neural crest stem cells (NCSCs) to migrate into the distal gut. We hypothesize that EDN3/EDNB-signalling is only involved in the migration of NCSC after embryonic day 12.5 during ENS development and is not necessary for proliferation, survival or differentiation of NCSCs in later stages. Thus, the migration defect caused by Ednrb deficiency could be bypassed by transplanting NCSCs directly into the aganglionic region of Ednrbsl/sl rats (spotting lethal rats that suffer of distal aganglionosis). We will analyze if transplanted NCSCs engraft and survive in the gut, undergo differentiation into functional enteric neurons and improve gut motor function. This study will provide evidence for stem cell replacement as a possible therapeutic tool.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Housing Association’s Energy Efficiency Process Planning and Investments' in the context of a Horizon 2020 call.

assistant: **Objectives**

The overall objective of the here proposed project “Housing Association’s Energy Efficiency Process Planning and Investments” (HAPPI) is to increase the energy renovation rate within the social housing sector using an exemplary action bringing together six social housing associations in the Danish municipality of Sonderborg. It will challenge the complex interplay of non-technological barriers (organizational, legal, financial) through a well-defined process and capacity building, leading ultimately to an aggregated 15,2 MEUR investment program (leverage factor 15,1) for sustainable energy measures in the associated building stock.The total investment in energy efficiency will include 56 different departments of the six social housing organizations’ building stock, involving a total of 6,000 tenants in 3,318 homes, of which 2,100 are flats in storey buildings and 1,208 are terrace houses.The investment launched by HAPPI is anticipated to lead to energy savings of 11,5 GWh/year, annual reductions of 1,640 tons CO2 eq. and the generation of 1.6 GWh/year of renewable energy.


**Keywords:** Housing associations, Innovative third party financing, Energy strategies, Capacity building, innovation, Tennants involvement, investments in 57 departments whit 3440 homes
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Seventh Framework proposal on 'The role of Sox2 in epithelial homeostasis and cancer'.

assistant: **Innovation**

The Sox family of transcription factors are versatile regulators of stem and progenitor cell fate. Sox2 is a well-characterized member of this family and plays essential roles during animal development and in adult stem cells. Accumulating evidence suggests that dysregulation of Sox2 expression is involved in key steps during tumorigenesis. However, the underlying molecular mechanisms of Sox2 in tumor formation and the Sox2 target genes mediating these phenotypes remain elusive.Here, using mouse skin as a model tissue, I propose to systematically profile the Sox2 regulatory network by RNA seq and ribosomal profiling. To determine the physiological relevance of Sox2 target gene changes, I plan to carry out an shRNA screen to uncover novel mediator of Sox2-dependent tissue growth. I will take advantage of the ultrasound-guided in utero microinjections of lentivirus into the amniotic cavity, which has been recently developed in our lab and allows to test the function of thousands of genes simultaneously. I will confirm and document the role of novel targets in mouse and human squamous cell carcinomas. Understanding how this stem cell regulator controls these key steps during tumor progression could be exploited not only for cancer diagnostics, but also in designing novel cancer therapies.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Linking Intestinal Bacteria and Host Metabolism to Tackle Type 2 Diabetes with Novel Food' in the context of a Horizon Europe call.

assistant: **Objectives**

Poor nutritional regimes are main drivers of metabolic diseases and intestinal dysbiosis. Type 2 diabetes (T2D) prevalence is expected to reach 7% of the global population by 2023, and to curb its rise is an urgent public health need. Intestinal dysbiosis is a determinant factor in the progression of insulin resistance to T2D, shifting host metabolism though undefined mechanisms whose understanding would be crucial to allow personalized interventions. Advances have been limited by microbiome complexity and inter-individual variance. DiBaN binds together the necessary combination of expertise to address this question, based on the concept that the initial driver of the nutritional effects is the metabolic shift that takes place in the intestinal bacteria which is transduced to the host. In this context, technological breakthrough tools for novel food development, that ensure the promotion of a healthy microbiome-host metabolic interface, are an urgent need to prevent dysbiosis and T2D. DiBaN will overcome current limitations in nutrient-testing by developing advanced ex-vivo platforms that fully recapitulate the in vivo setting of dysbiosis and insulin resistance, that will be validated with in vivo omic data. To warrantee the health promoting effects of novel foods we will test a new concept in the emerging field of insect food technology, that an adequate insect’s metabolic-intestinal health ensures the healthy properties of its derived products. The validation of this idea will also serve to identify biomarkers for the monitorization of the insect’s health status. A. domesticus will be the testing model of choice, due to its excellent nutritional profile, that will be boosted by complementing the insect’s diet with microalgae bioactive-rich extracts. All these data wi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Exploring the process of urbanisation in the Roman provinces of Germania through the study of food plant commerce' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

The arrival of the Romans in large parts of current Europe had a major impact on society, with the creation of a new transport network and the development of cities being two of the most significant and lasting changes. However, so far, little work has been done to quantify and understand their connection, how this developed and its lasting consequences. Through the study of imported/introduced food plants into the provinces of Germania in the Roman period, PlantNetGem aims to contribute to a better understanding of foodways and the role of transport and commerce in the development of the population nuclei in this area. It will apply a novel, interdisciplinary approach, combining archaeobotany, computational archaeology and data science through practical training by the supervisory team that pioneered part of the proposed methodology. The project will start by creating a geodatabase where archaeobotanical and archaeological information from the study area will be stored. These will provide the analytical basis to identify which food plants arrived and when, who had access to these and the reasons why. Network science will be applied to reconstruct settlement connectivity in relation to a) their access to the introduced food plants, and b) their position within and access to the Roman transport network. Agent-Based Modelling and statistical analyses will be applied to further allow testing hypotheses concerning settlement position and hierarchy in relation to commerce to ultimately evaluate the role of the ancient transport network in the distribution and importance of urban centres and analyse to which degree these patterns have endured in time up to the modern period. PlantNetGem builds upon the candidates solid expertise and involves training on big data management, n