RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Nonwovens forming process with spunlaced fibres & new system - h/w & s/w - to achieve such a process' (INNOTEX) targeting Fourth Framework.

assistant: **Abstract**

Objectives and content The purpose of the project is to produce SPUNLACED NONWOVENS by using air jets instead of water jets. It is therefore necessary to study all suitable machinery and plants to achieve the new process. This process is basically innovating because the kinetic action is obtained through the air, as water is only a transient mean for energy transfer. Moreover water consumption is very low: water must not be recycled and the traditional expensive recycling depuration plants are not necessary. This new technology, through a lower mechanical stress of fibres, will allow:  use of innovating fibres having high technological content (alternative to the natural fibres), as well as the use of materials different from general fibres (special fibres such as glass, ceramic, carbon, silicon. etc.);  utilisation of cheap synthetic fibres too, that are usually used only in dry processes because in the known spunlaced process the fibre sizing pollute quickly the recycling water circuit; on the contrary in the innovative process, as the recycling is not necessary, the sizing remain in the water and it is enough to separate them through flocculation before eliminating the same water, by means of simple and low operating cost plants;  to enlarge the range of nonwovens that can be produced as far as the weight is concerned. The product will have a textile, flexible and clothing aspect, but it will have mechanical resistance (i.e. 250 NW for 60 gr./sq.m) that will be suitable for use in the most skilled fields of converting techniques and particularly:  aeronautical and missilery.  automotive and high speed.  technical products for the struggle against the air and soil defilement.  safety of working places.  communication infrastructures. Also the management of new process
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Labeling Flavivirus capsid protein to unravel its dynamics during infection and egress by correlative super-resolution fluorescence and cryo-electron microscopy' to be submitted under Horizon 2020.

assistant: **Abstract**

Vector-borne diseases are major worldwide threats. Specifically, mosquito-borne diseases from the flavivirus genus such as West Nile and dengue viruses are raising major concerns on human health. There are few or no treatment/vaccine strategies to fight these viruses and the relatively poor knowledge of their life cycle (entry and egress) contributes to the deficit in treatment possibilities. A deeper knowledge of the virus cycle is required to unlock the development of antivirals.Understanding the dynamics of the viral proteins and their interactions in vivo strongly relies on quantitative and high resolution imaging methods. These methodologies in turn require specific protein-labeling strategies, which are presently quite limited for flavivirus. Significant advances in understanding flavivirus biology can be expected if such labeling tools were available.The major goal of this project is to understand the delivery of the flavivirus genome to its translation site at the cytoplasmic side of the ER membrane; the cellular components and machineries that intervene and the role and paths of the Capsid protein during this process. Super-resolution PALM/STORM techniques will be used and superposed to cryo-EM data and the yellow fever virus attenuated 17D strain will be used as model flavivirus for the main project milestones. We will devise and optimize new labeling strategies for capsid protein and use them to track the virus in vivo and follow the path of the capsid protein after release from the virus during cell entry.The project, thus aims to provide a labeling tool that will boost flavivirus research and to advance our knowledge of flaviviruses’ life cycle.


**Keywords:** Flavivirus, Capsid protein, Protein labeling, Super-resolution microscopy, single particle PALM,
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Role of cell mechanics in tumour metastases formation' (ROLECELLMEC) under Sixth Framework.

assistant: **Objectives**

The proposed project is a continuation of the current Marie Curie Intra-European Fellowship project. In the project the role of cellular mechanics in the formation of cancer metastases will be studied. Metastasis, the formation of secondary tumours, is the most feared and least understood aspect of cancer.Regarding the commonness of metastases formation, a detailed view on the process of metastases formation reports a very complicated cascade of processes. For a cancer cell to metastasize, it must first detach from the parent tumour. Then, cancer cells have to penetrate tissues that provide only micrometer large holes for cells to penetrate.Usually, these cavities in tissues are much smaller than the size of a single cancer cell. Thus, cells have to deform strongly which may only be explained if these cells adapt their mechanical properties for tissue penetration. Numerous processes in the cells, like mitosis, intracellular traffic of organelles are mediated by cell mechanics, more closely by the cytoskeleton.We have demonstrated that the naturally occurring sphingosylphosphorylcholine (SPC), which occurs more abundantly in metastasized cancer cells and lysophosphatidic acid (LPA) modify the cytoskeleton, thus the mechanical properties of the cells. In the proposed project we will further study the mechanical properties of cells extending the selection of biochemical treatment, not only on fixed and stained cells, but also on living, fluorescently transfected cell lineages.We want to analyse quantitatively the contribution of the various cytoskeleton filaments to the mechanical properties of the cells and draw clinical consequences regarding the formation of metastases from cancer tissues. We are aiming to a truly interdisciplinary research project which involves bioche
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Adaptation and Mitigation Strategies: Supporting European climate policy' under Sixth Framework.

assistant: **Concept**

The core objectives of ADAM (ADaptation And Mitigation) are:To assess the extent to which existing and evolving EU (and world) mitigation and adaptation policies can achieve a tolerable transition (a 'soft landing') to a world with a global climate no warm er than 2 degrees C above pre-industrial levels, and to identify their associated costs and effectiveness, including an assessment of the damages avoided compared to a scenario where climate change continues unchecked to 5 C. To develop and appraise a port folio of longer term strategic policy options that could contribute to addressing identified shortfalls both between existing mitigation policies and the achievement of the EU's 2 C target, and between existing adaptation policy development and implied EU goals and targets for adaptation. To develop a novel Policy-options Appraisal Framework and apply it both to existing and evolving policies, and to new, long-term strategic policy options, so as to inform: European and international climate protection stra tegy in post-2012 Kyoto negotiations, a re-structuring of International Development Assistance, the EU electricity sector and regional spatial planning.The ADAM project will lead to a better understanding of the synergies, trade-offs and conflicts that exi st between adaptation and mitigation policies at multiple scales. Crucially, ADAM will support EU policy development in the next stage of the development of the Kyoto Protocol, in particular negotiations around a post-2012 global climate policy regime, an d will inform the emergence of new adaptation strategies for Europe. The main impact of the ADAM project will be to improve the quality and relevance of scientific and stakeholder contributions to the development and evaluation of climate change policy op tion
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Investigating the functional impact of neurodegeneration-causing rare variants in the teleost model zebrafish' (INNERVATE) under Horizon 2020.

assistant: **Objectives**

Circa 30 million people in Europe suffer from life-threatening rare diseases, which are poorly understood. This major societal burden forces patients into endless paths for a diagnosis often without cure. EU cooperation efforts between countries contribute to improve research and visibility of these diseases with a primary role of the ‘Ospedale pediatrico Bambino Gesù’ (OPBG, Italy). Here, via next generation sequencing technology, Dr. Tartaglia recently described new genetic variants, including some in the tubulin cofactors TBCE and TBCD which alter microtubules (MTs) dynamics (crucial for brain development and maintenance) and cause previously undescribed early onset neurodegeneration. Yet the underlying pathogenic mechanisms and impact on the brain physiology remain elusive. Given the difficulty to reproduce brain physiology in vitro and its general inaccessibility, a vertebrate model amenable to genetic and whole-brain in vivo investigation is necessary. With its rapid and transparent development and genetic and imaging tools improving continuously, the small teleost fish zebrafish is ideal. I will monitor live MTs behaviour and cellular processes (e.g. cell division, axons formation and innervation) in the precursor and mature neurons of zebrafish TBCE and TBCD mutants, focusing on motoneurons. To this aim, I will combine my expertise in fish neurobiology with the host’skills in human genetics (Dr. Tartaglia) and fish gene editing (Dr. Del Bene, France). In line with the current EU efforts, this work will advance our understanding on rare genetic variants causing neurodegeneration. Deciphering the role of MTs dynamics on brain physiology will be relevant also for common brain disorders and exome sequencing of undiagnosed patients will further contribute to a genoty
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Role of endoplasmic reticulum in neurodegeneration: physiopathology of a form of hereditary spastic paraplegia as a model' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

Endoplasmic reticulum (ER) is a dynamic, tubular intracellular network implicated in a variety of cellular functions. Although it is known to play a role in neurodegeneration, its organization and precise function in neurons have been neglected. I will use the physiopathology of certain forms of hereditary spastic paraplegia (HSP) as a model to understand why and how alteration of ER dynamics in neurons can lead to neurodegeneration.I will focus on three genetic entities, SPG11, SPG15 and SPG48, which are clinically and biochemically related, since the proteins encoded by these genes (spatacsin, spastizin and KIAA0415) are all present in a multiprotein complex important for ER function. The project has three objectives:-Elucidate the role of spatacsin, spastizin and KIAA0415 in the regulation of ER morphology and dynamics;-Establish the link between abnormal ER function and neurodegeneration;-Identify new partners of spatacsin, spastizin and KIAA0415 implicated in ER function.-We will first develop and characterize physiopathological experimental models: (i) an  SPG11 knockout mouse; (ii) primary cultures of neurons subjected to RNA interference to downregulate expression of the SPG11, 15 and 48 genes;  (iii) neurons differentiated from induced pluripotent stem (iPS) cells derived from fibroblasts of patients with mutations in SPG11, 15 and 48. ER organization and dynamics will be analyzed in these models using electron and fluorescence microscopy (confocal microscopy, super resolution Structured Illumination Microcopy), as well as videomicroscopy on living cultured neurons. The cell culture models will allow us to determine how the functions of ER that are altered in the pathological models in culture are linked to neuronal death. This pioneering work will help underst
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Plant-Bacillus Interaction: Gain of Function Project' goes beyond current state-of-the-art under Horizon 2020.

assistant: **Innovation**

New and sustainable methods must be developed to meet society’s ever-increasing demand for food, energy, and chemicals. Feeding tomorrow’s population requires increasing food production sustainably and developing novel approaches to invert the excessive consumption growth of fertilizers, pesticides, and irrigation-water by modern agriculture. Fortunately, symbiotic or non-symbiotic interactions between plant-hosts and specific microbes were shown to promote host-vigor, and improved product quality and yield and plant-growth-promoting bacilli have been commercialized as biofertilizers in recent years. However those benefits associated to the PGPRs are divided across a large diversity of bacteria; and host-plants demands, nutrient availability, and pathogen attacks vary constantly during the entire plant lifespan.      The proposed research project for the H2020-MSCA-IF-2014 will focus on identifying, validating, and stacking bacterial gene clusters responsible of those properties. The approach consists of establishing libraries of hybrid-bacillus and screening them for novel plant growth promoting properties. Because of the current importance of reducing inputs in agriculture while increasing crop yield; the screen will focus on identifying properties that allows decreasing use of chemical fertilizers such nitrogen and phosphorus and improve drought and salt tolerance. The proposed research intertwines fundamental and applied fields and is designed to seed larger projects toward a sustainable agriculture and feed tomorrow’s population. It is designed to increase our knowledge in plant-microbe interactions, result in discoveries, and generate innovative technologies.


**Keywords:** Bacillus subtilis, Bacillus amyloliquefaciens, Hybrid-bacteria, Plant growth promoting bac
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Dissecting Macrophage Mechanobiology to Engineer Immuno-Regenerative Biomaterials' (MACxercise).

assistant: **Concept**

There is an increasing clinical demand for sophisticated medical implants and the scientific field of implant technology is exponentially growing. The main challenge is to harness the immune response to such an implant. In this research we use the immune response to our advantage, by using bioresorbable synthetic biomaterials that are gradually replaced by living tissue inside the body. One of the key immune cells are macrophages, which are the gatekeepers for successful implant integration. Strategies to harness the macrophage response focus on isolated biomaterial design features, such as biochemical or microstructural modifications. One major neglected factor is how macrophages sense and respond to mechanical loads, such as cyclic stretch, or ‘macrophage mechanobiology’. Without an in-depth understanding of macrophage mechanobiology, rational engineering of biomaterials is not possible, leading to unpredictable outcomes and ineffective trial-and-error work. With MACxercise, I will leverage my unique multi-disciplinary expertise in bioengineering, biomaterial science and macrophage biology to address these scientific challenges from a new angle in which macrophage mechanobiology is placed center stage. The Main Aim of the MACxercise program is to systematically dissect how macrophages respond to dynamic mechanical cues and to establish how this affects biomaterial-driven tissue regeneration. The transformative strength of MACxercise lies in the concerted spatial and temporal manipulation of the macrophage microenvironment, using sophisticated engineering tools to decouple the mechanical and physical cues to systematically pinpoint how synergistic or conflicting cues dictate key macrophage functions in the biomaterial microenvironment. With MACxercise, my vision is to
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Death Receptors as Integrators of Stress-induced inflammation' goes beyond current state-of-the-art under Horizon 2020.

assistant: **Innovation**

Inflammation is initiated in response to the detection of foreign entities, called PAMPs derived from infectious agents, or due to the release of intracellular contents, called DAMPs, due to serious tissue damage (i.e. necrosis). However, inflammation can also be initiated in response to Cell Stress (e.g. ER stress, cytoplasmic stress, mitochondrial stress) resulting from perturbations in normal physiology, but how cellular stress is converted to inflammatory outputs is very poorly understood. Many diseases are associated with chronic long-term inflammation (e.g. cancer, obesity, neurodegeneration, diabetes), which is a compounding factor in these conditions that can accelerate disease progression, but the inflammation seen in these conditions does not have an obvious infectious (PAMP) or acute injury (DAMP) cause. Instead, these diseases are frequently associated with persistent cell/tissue stress (e.g. due to misfolded proteins, elevated dietary fats, or metabolic stress) arising from persistent ER or cytoplasmic stress, that is likely to serve as a key driver of inflammation in these settings. However, the key sensors and effectors of stress-induced inflammation remain enigmatic.   Based on our recent observations, I wish to explore the hypothesis that members of the 'Death Receptor' subset of the TNF receptor family serve as stress-associated molecular patterns (SAMPs), becoming upregulated and/or activated in response to divergent forms of ER and cytoplasmic stress, leading to inflammation. Here, we will explore the role of Death Receptors as putative SAMPs, how they are activated by stress, the composition of their stress-induced signaling complexes, and the potential to suppress stress-induced inflammation through targeting these receptors.  Understanding how Cel
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'The roots and evolution of the culture-of-death. A taphonomic research of the European Paleolithic record' demonstrating excellence, impact and implementation quality for a Horizon 2020 call.

assistant: **Abstract**

There can be no more profound insight into the human mind than the complex cultural practices surrounding death. While all human cultures across the globe today engage in funerary practices, the emergence of funerary behavior is one of the most contentious aspects in the field of human evolution. New methodological approaches on taphonomy field can help elucidate fundamental facets of hominin behavior making important contributions to the understanding of our ancestors. The European fossil record is a key source of information in this regard due to the relative abundance of fossil skeleton, many of which have been interpreted as burials, and the possibility for biological and cultural interactions between different human species. Nevertheless, direct taphonomic analyses on these human fossils are rare and essentially limited to those cases with possible signs of cannibalism. The present application proposes a multidisciplinary research project to investigate the origin of funerary behavior during the Middle Pleistocene and to trace this behavior throughout the European Paleolithic archaeological record. This project aims to address the dearth of taphonomic studies on Paleolithic hominins and represents the first large-scale project focused on a thorough multi-taphonomic study of the European fossil record. This involves the participation of a wide team of scholars and a network of methods including classical and innovative taphonomic analyses, virtual reconstructions for forensic analysis, study of spatial distribution patterns, the global relationship of different sites, and mathematical models to interconnect the broad-spectrum data gathered. The results have the potential to significantly alter our views on behavioral aspects of European Paleolithic populations. In p