RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Molecular mechanisms of transcriptional regulation of lymphocyte development by the E2A splice variants E12 and E47' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

The E2A gene encodes for two alternatively spliced proteins, namely E12 and E47, which belong to the family of basic helix-loop-helix transcription factors. The E2A proteins have been characterized extensively as regulators during various stages of B and T lymphocyte development. Recently, we have generated mice deficient for either E12 or E47 and examined their roles in B cell maturation. We show that E47 is essential for developmental progression at the pre-pro- B cell stage, while E12 is dispensable for early B cell development, commitment and maintenance. In contrast, both E12 and E47 play critical roles in pre-B and immature B cells to promote Igλ germline transcription as well as Igλ VJ gene rearrangement. However, it is unclear which molecular mechanisms are used by E12 and E47 to drive lymphocyte development. Thus, the goals of this study are to 1. identify the signalling events that control transcription and splicing of the E2A gene, 2. define individual target genes and interaction partners of E12 and E47 and 3. to examine the distinct functions for E12 and E47 during T cell development. These goals will be accomplished using the previously generated mouse models, lacking either the E12 or E47 protein in combination with cellular and molecular biology approaches. Additionally, the first goal will be approached in cooperation with the applicant’s previous lab, since this lab has extensive experience with the proposed state-of-the-art whole genome Chromatin Immunoprecipitation combined with deep sequencing (ChIP-Seq) approach. Overall, this project will contribute to our understanding of the molecular mechanisms of transcriptional regulation as well as lymphocyte development.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Precision engineering for plant resilience: MYB-driven tissue-specific deposition of essential plant biopolymers' under Horizon Europe.

assistant: **Concept**

Environmental stressors such as nutritional imbalances, severe temperature, drought, and salinity are detrimental to plant growth. As a major strategy for adaptation to environmental stressors, plants have evolved extracellular barriers as part of their cell walls made of aliphatic biopolymers, cutin and suberin, along with their associated waxes. Cutin and cuticular waxes form the cuticle that covers the outer surfaces of leaves, fruits, and flowers. Suberin, on the other hand, is present in the extracellular space of inner tissues, such as endodermis, periderm, and exodermis, as well as the seed coat. Both cutin and suberin act as barriers, preventing water loss and nutrient leakage while also shielding the plant from pathogens. Despite decades of studies on these biopolymers, many essential aspects of their biosynthesis and assembly remain unclear. Among these is our understanding of the assembly of cuticle and suberin monomers in the extracellular space, as well as the control of the polymerising machinery both developmentally and environmentally. A few members of the GDSL-esterases/lipases (GELP) family have been shown to be involved in the polymerisation of tomato fruit cuticle and Arabidopsis thaliana root suberin. Intriguingly, another subset of GELPs has been shown to be able to degrade cutin or suberin, implying a complex mechanism that plants have evolved to modulate the dynamics of these polymers. On a regulatory level, several MYB transcription factors have been shown to be important in controlling the developmental and tissue-specific responses of suberin and cutin. The proposed research aims at uncovering the mechanisms of MYB-directed and GELP-mediated cuticle and suberin assembly and plasticity during growth and adaptation. Importantly, as part of our p
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Center for biomolecular chemistry (CBCH)', explaining its relevance to EU policy priorities under Fifth Framework.

assistant: **Impact**

The proposal is to restructure the scientific activity within the Centre by(I) establishing new links with European research centres(ii) elaboration of novel networks with participation of European and Hungarian S&T institutions;(iii) formation of new co-operations with European academy; and(iv) organising courses. Workshops and conferences in order to intensify the research and training activity or the Centre on chemistry of bioactive materials and thereby provide a substantial contribution to achieve results meeting the economic and social needs of the region including impact to industrial applications. The activity of the proposal covers the following research areas: free radical generation and cell attachment, mass spectrometry-based peptide sequencing, protein aggregation, synthesis of new bioactive heterocyclic compounds, development of neuroprotective strategies
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon 2020 proposal for the project 'Enhancing the innovation management capacity of Finnish SMEs by the Enterprise Europe Network in 2017-2018'.

assistant: **Objectives**

Lack of innovation management capacity is recognised as an important barrier to creating economic impact (competitiveness, growth and jobs) from innovation activities in SMEs. The general objective of the proposal is to achieve overall long term capacity building in (Finnish) SME’s, particularly to make the innovation system in the companies more efficient and effective and to increase and accelerate the economic returns from innovation in the SMEs. Actions will be identified to address weaknesses in the innovation capacities of the SME that hinder the realisation of growth opportunities and of the full commercial potential. Hence, the improvement of this innovation management capacity upgrades the economic performance of the most promising SMEs.First specific objective of the project is to provide Key account management services to beneficiaries of the SME instrument. These services aim to identify bottlenecks to the creation of economic impact in companies benefitting from grants by Horizon2020's SME instrument. Based on the needs analysis the Key account management services identify suitable coaches to address the identified bottlenecks and also facilitates the coach-client relationship during the coaching project. Second specific objective is to provide services, which enhance the innovation management capacity of SMEs. This service will increase the efficiency and effectiveness of innovation processes in small and medium sized enterprises with significant innovation activities that could potentially become beneficiaries of European support to research and innovation but would not have effective access to consulting services for innovation management. The service includes an assessment of innovation management capacity and development actions based on the assessment
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Aluminium and Magnesium Alloys Green Innovative Coatings' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

The aeronautic industry is constantly striving to reduce the aircraft operating costs, increase their payload and reduce the environmental impact during the whole life of the product. Metallurgy has played a key role in the development of aviation. With the use of light alloys, as aluminium and magnesium, new applications have been found to apply these to fastly improve existing designs.The disadvantage of using these materials is that they are particularly susceptible to corrosion. Environmental degradation is a limiting factor for magnesium–aluminium (Mg–Al) alloys in outdoor applications. An effective way to protect alloys from fast degradation or reduce to the degradation rate is surface treatment. Hexavalent chromium has served as the primary means of corrosion protection in the aircraft industry since 1936 and allowed for the distinctive bare-metal finishes of the World War II era. Hexavalent chromium is a known carcinogen, with the major route of exposure through inhalation of vapours or dust. The chromates are among the current chemicals for which industrial users must find substitutes, or request authorisation from EU regulators to continue their use. In the case of chromium trioxide and the acids, the application deadline is March 2016 and the “sunset” date for the substances is September 2017.Therefore, there is an urgent need facing the aerospace industry to replace the conventional corrosion inhibitor, hexavalent chromium. Regulatory and market drivers are motivating a global effort in the aerospace industry to replace hexavalent chromium-containing materials with hexavalent chromium-free alternatives for various applications.ALMAGIC project is focused on solving the aforementioned problematic by validating the developed innovative alternatives to chromium(
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Vaccine lot to Vaccine lot comparison by consistency testing.' to be submitted under Horizon 2020.

assistant: **Abstract**

Lot release testing of established vaccines relies on using animal models. Many of these models were developed decades ago and reflect the level of product knowledge, production technology and quality management in those days. Current production techniques are optimised, in-process control is implemented and tight quality management systems are in place. These developments have brought testing for lot-to-lot consistency into reach. The consistency approach involves the use of a set of parameters obtained by using innovative in vitro technologies, to define the product along the manufacturing pathway and to ensure similarity to a lot of proven efficacy and safety.The ambition of the VAC2VAC project is a demonstrated proof of concept of the consistency approach for lot release testing. We will develop/optimise and validate innovative in vitro methods for the categories of established vaccines on the market. Product profiles will be defined using analytical methods, cell-based assays and bioinformatics.The project includes 7 WPs: 4 on R&D, and one each on validation, promotion to regulatory acceptance and consortium management. Consortium partners represent the vaccine stakeholder groups (OMCLs, Academia, Translational research institutes and Vaccinology alliances) and are complementary for the expertise ranging from method development and validation to facilitating and providing guidance to regulatory acceptance. Acceptance will be promoted by strategic guidance activities: a roadmap conference, workshops and training courses. Implementation of the consistency approach in lot release testing has multiple impact: 1) strengthening fundamental understanding of vaccine products resulting in enhanced understanding of vaccine quality 2) strengthening competitiveness of European
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon Europe proposal on 'Universal Cardiac Mesoangioblasts for treating  DMD Dilated Cardiomyopathy'.

assistant: **Concept**

Dilated cardiomyopathy (DCM) is the second most common cause of heart failure, currently treated with drugs that delay progress towards heart transplantation. There are currently many attempts to treat DC with stem cells or their extracellular vesicles or AAV vectors: none reached efficacy so far. The applicant has a long track record in cell and gene therapy for muscular dystrophy. He pioneered systemic intra-arterial transplantation of mesoangioblasts (blood vessel-derived progenitors) and, thanks to a previous ERC grant, succeeded in creating immortal, universal donor mesoangioblasts. Edited cells do not activate an immune response in vitro or in vivo. Muscular dystrophy also affects the heart causing a DCM, but a simple extension of this strategy is problematic since the existence of resident cardiac stem cells is controversial and cardiac mesoangioblasts do not spontaneously differ-entiate into cardiomyocytes. iPS cell-derived cardiac progenitors are promising but until now for lo-calised lesions such as myocardial infarcts. To address this problem we will produce immortal, im-mune privileged cardiac mesoangioblasts and will convert them to cardioblasts, in vitro expression of cardiac transcription factors. Since conversion takes about two weeks, we will test different settings to allow cells so that they would home and differentiate in vivo in the areas of damage, characterized by inflammation. The applicant is in the unique position to test feasibility of this project for future translation into a novel clinical protocol.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'The role of the Jak tyrosine kinases in the trafficking of the type I interferon receptor', explaining its relevance to EU policy priorities under Sixth Framework.

assistant: **Impact**

The type l interferon receptor belongs to the class II cytokine receptor family and is expressed on all cell types as a heterodimeric complex that consists of the IFNAR1 and IFNAR2 subunits. Binding of interferon a/ß to its receptor triggers phosphorylation of associated tyrosine kinases of Jak family, Tyk2 and Jak1, and subsequent downstream signalling events. Recent findings have shown that Jak protein tyrosine kinases can control cellular responses to cytokines by influencing receptor localization, modulating their surface expression levels, and therefore the formation of functional high affinity receptors.Based on initial studies with IFNAR1 and Tyk2, which revealed Tyk2 dependent IFNAR1 surface expression, I propose to study the role of Tyk2 in ligand dependent events. I will focus in particular on the potential role ofTyk2 in ligand-dependent IFNAR1 trafficking. A second aspect of the study will take into account IFNAR2 subunit of the type l interferon receptor and its associated tyrosine kinase Jak1. I propose to examine whether IFNAR2 surface expression is Jak1 dependent and to check whether and how does it correlate with expression of IFNAR1.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Fourth Framework proposal on 'Population ecology and behaviour of goshawks accipiter gentilis in relation to forestry practices and habitat change'.

assistant: **Concept**

Scandinavian goshawk populations have seriously declined this century, apparently as a consequence of changes in forestry practice altering their habitat, and the species is now classified as threatened. Despite being an important indicator species, basic data on critical areas in goshawk behaviour and life history, particularly the period between the first winter and establishment as a breeding adult, are completely lacking and are required before a conservation strategy can be developed. The proposed project is a part of an interdisciplinary research programme investigating forestry practice and its effects. Radio telemetry and ringing databases will be used to examine in detail behaviour, habitat use, and recruitment processes in goshawks. This information will provide vital basic data on the behaviour and population ecology of the species, identify critical habitat types, and, integrated with the wider programme, examine modifications to current forestry practices which would assist the conservation of this and other species.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Comprehensive and systematic study of the acetylation function of acetyltransferases in human cells' (ACETO-SAGATAC-HUMAN).

assistant: **Innovation**

Histone acetyltransferases (HATs) play a critical role in regulating gene expression of many cellular functions. Interestingly, the vision of histones being unique substrates for different HATs has recently changed, since HATs can also regulate essential functions such as protein turnover or cell cycle progression, via acetylation of non-histone proteins. HATs and their acetylation activity must have multiple physiological roles, which are still poorly understood. This is especially the case of GCN5 and PCAF, which are homologous HATs, but have different roles in vivo. Because a fine balance between acetylation and deacetylation regulates many cellular functions and the smallest change in this balance can lead to cancerogenesis, it is extremely important to understand the global acetylation function of HATs. In order to get more insights into functional role of HATs and acetylation in human cells in vivo, we propose to identify substrates of the human HATs, GCN5 and PCAF using systematic proteomic analysis. The targets of these HATs, either as recombinant enzymes or within their associated  protein complexes, SAGA (Spt-Ada-Gcn5 acetylase) and ATAC (ADA Two-A containing Complexe), will be screened performing acetyltransferase assay on a human protein array containing more than 9000 proteins. Changes in acetylome will be also compared in between wild-type and cells lacking GCN5/PCAF using cutting-edge proteomic methodology such as Multidimensional Protein Identification Technology (MudPIT). Once the specific non-histone targets of these HAT complexes will be defined by the combination of these methods, we will carry out physiological studies to understand the in vivo regulatory role of these acetylation patterns determined by the SAGA and/or ATAC complexes. The success of
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Advanced nanospectroscopy for mapping local conductivity in conductive metal-organic framework' (NAPOLEON) targeting Horizon Europe.

assistant: **Abstract**

NAPOLEON’s main objective is the development and demonstration of mapping ultra-broadband optical conductivity of novel conductive metal-organic frameworks (MOFs) by infrared nanospectroscopy (nano-FTIR). Conductive MOFs are critically important for the development of a highly efficient and low cost electrocatalysis for electric energy storage and retrieval. MOFs development needs the understanding of its conductive properties down to the nanoscale, which is not possible with traditional electrical transport measurements. Nano-FITR offers several advantages compared to traditional transport measurements: no contacts are needed, there are no problems with contact resistances, nanoscale materials can be measured, and the frequency-dependent response provides deeper insights into conduction properties than just DC measurements. However, current tabletop nano-FTIR systems are not able to characterise conductive MOFs due to the limited spectral coverage of the employed laser sources. To overcome this challenge I will introduce a novel plasma source-based nano-FTIR spectrometer, which will ensure the broadest spectral coverage. Then, I will develop a new model from which the anisotropic conductivity of MOFs can be extracted from nano-FTIR spectra. Finally, I will use the improved nano-FTIR system and the new model to study a highly conductive MOF sample, the CU-BHT, which demonstrated high electrocatalytic activity. NAPOLEON will provide innovative technologies, original methods and breakthrough knowledge in the fields of chemistry, nanooptics and solid-state physics. Specifically, NAPOLEON will develop the first methodology to measure optical conductivity in conductive MOFs with nanoscale resolution, which will contribute to boost the development of these materials. The resu