RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Crystallographic and biochemical studies of the human TBX5-Nkx2-5-DNA complex' (TBX5-NKX2) under Seventh Framework.

assistant: **Objectives**

Holt Oram syndrome (HOS) is a heart and limb syndrome, which manifests itself in 1 of 100000 live births. HOS syndrome is an autosomal-dominant disease, where forelimb and cardiac congenital abnormalities are observed. The syndrome is connected to mutations in the T-box transcription factor TBX5 gene. Similar symptoms are observed when mutations in the homeobox transcription factor human Nkx2-5 are present. TBX5 and Nkx2-5 interact with each other and synergistically activate the promoters of the cardiac-specific natriuretic peptide precursor type A (ANF) and connexin (cx40). The project goal is the biochemical characterization and crystal structure solution of the ternary TBX5-Nkx2-5-DNA complex. The crystal structure of the ternary complex will allow a comprehensive structural analysis of the interaction mechanism between TBX5 and Nkx2-5 and their DNA binding sites and will elucidate the synergistic promoter activation of TBX5 and Nkx2-5 on a molecular level. Such insight is of broad interest for cardiovascular biologist because it opens the ultimate perspective to use this knowledge for influencing cardiac growth and for cardiac regeneration. The results of this project will contribute to the European quality and competitiveness in cardio vascular research. The project uses the full portfolio of structural biological research and requires interdisciplinary knowledge in molecular biology, biochemistry, biophysics, protein crystallography and bioinformatical techniques. Thus, the researcher has the possibility to gain skills in all techniques necessary for doing successful research in structural biology. In addition, the multidisciplinary environment at the EMBL will give the researcher the possibility to interact and communicate with scientists in fields other than cr
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Characterization of the molecular structure of a novel g protein-regulated phosphoinositide 3-kinase' in the context of a Fourth Framework call.

assistant: **Objectives**

Phosphoinositide 3-kinases (PI3K)s regulate many cellular responses. The host laboratory has identified a novel PI3K in myeloid cells which, unlike known PI3Ks, is not activated by receptor protein-tyrosine kinases, but by the BY subunits of heterotrimeric G proteins. The enzyme is a heterodimer of a 120 kDa and a 101 kDa protein; both subunits are now cloned. This proposal is aimed at characterizing the molecular structure of the novel PI3K, its regulation by ISY subunits, and its involvement in cellular signalling. The project is based on the generation of deletion mutants of both PI3K subunits for definition of the molecular basis of their interaction, the catalytic activity, and their interaction with BY subunits. We shall introduce the enzyme into mammalian cells by transient transfection to determine its effect on cellular signalling and we shall attempt to generate dominant negative forms of the PI3K for selective inhibition of G protein BY subunit-mediated pathways.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Principles of Chromatin Organization', explaining its relevance to EU policy priorities under Seventh Framework.

assistant: **Impact**

Chromatin is the ensemble of genomic DNA and hundreds of structural and regulatory proteins. Together these proteins govern the gene expression program of a cell. While biochemical and genetic approaches have tought us much about interactions between individual chromatin proteins, we still lack a “big picture” of chromatin: how is the entire interaction network of chromatin proteins organized?My lab discovered that chromatin in Drosophila consists of a limited number of principal types that partition the genome into domains with distinct regulatory properties. Among these is BLACK chromatin, a novel repressive type of chromatin that covers nearly half of the fly genome. It is still largely unclear how these different chromatin types are formed, how they are targeted to specific genomic regions, and how they interact with each other.Here, I propose a combination of systematic approaches aimed to gain insight into the basic mechanisms that drive the partioning of the genome into distinct chromatin types. New genomics techniques, developed in my laboratory, will be used to construct an integrated view of the interplay of more than one hundred representative chromatin proteins with each other and with sequence elements in the genome. Specifically, we will: (1) Study the genome-wide dynamic repositioning of chromatin domains during development in relation to gene regulation; (2) Use a novel and versatile parallel genome-wide reporter assay to dissect the interplay among DNA sequences and chromatin types; (3) Combine computational modeling with a high-throughput genome-wide assay to uncover the network of interactions responsible for the formation of the principal chromatin types; (4) Dissect the molecular architecture of BLACK chromatin and its role in gene repression.The re
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Microfluidic Combinatorial On Demand Systems: a Platform for High-Throughput Screening in Chemistry and Biotechnology' under Seventh Framework.

assistant: **Concept**

This proposal addresses an important opportunity in the rapidly developing art of microfluidics. On one hand vast expertise is available on automation of single phase flows via microvalves or electrokinetics and on flow of drops on planar electrodes. These systems are perfectly suited for a range of applications but are inherently inefficient in handling massively large numbers of processes due to correspondingly large number of input/output controls that at best scales logarithmically in the number of processes. On the other hand conducting reactions in thousands micro droplets embodies many of the most acclaimed promises of microfluidics – ultra-miniaturisation, speed, rapid mixing and extensive control of physical conditions. Demonstrations of incubation of cells, in-vitro translation and directed evolution confirm that these techniques can reduce the cost and time of existing processes by orders of magnitude. Droplet microfluidics is at the moment, however, almost (except sorting) completely passive.We recently demonstrated the use of external valves to automate formation and motion of droplets on simple disposable chips and screening up to 10000 compositions per hour. We propose to develop externally controlled programmable modules for i) multiplexed, on-demand generation of multiple emulsions, ii) aspiration of libraries of samples and multiplexing linear libraries into full cross matrices, iii) splitting drops into two, few and large numbers (e.g. 10000) drops, iv) optical monitoring of presence and content of droplets, v) counting cells inside the drops, vi) circulating drops, vii) titration, viii) holding paramagnetic beads in drops. Our design rules will allow to integrate these modules into externally controlled systems for research on i) combinatorial synthe
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Development of a validated universal slip resistance test for pedestrian and workplace floor surfaces' in the context of a Fourth Framework call.

assistant: **Objectives**

- To establish a validated universal test method for measuring the slip resistance of floorings under dry, wet and contaminated conditions.- To identify or develop suitable portable test apparatus for in-situ measurement of flooring slip resistance according to the above method.- To determine the correlation between the defined method and the best available method for determining the slip resistance of footwear (EC contract MATI CT940059)It was found that the performance ranking of the benchmark floorings depended on both the contaminant and the shoe type, effectively giving four different rankings. This meant that the final test method would have to include a number of different sets of test conditions in order to give a meaningful representation of in-service performance.A range of test conditions was evaluated using versatile laboratory devices and a final draft test method was successfully derived. The defined approach was confirmed as giving more consistent and higher levels of correlation against the four rankings of the benchmark floorings than any of the other portable slip devices. The correlation coefficients ranged from 0.75 to 0.92. A standard plain soling slider material was developed for the test but it was beyond the scope of the project to develop a standard patterned slider. The RRL Pendulum performed well but cannot test patterned sliders.A new prototype portable test was designed and built to comply with the method. Initial trials with the device were encouraging but engineering modifications were required to eliminate vibration, which exceeded the resources and timeframe of the project. The test method is available to all CEN TC's who wish to consider adopting it. The RTD partners will provide laboratory testing services on a commercial basis and the
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'The Secret Life of Writing: People, Script and Ideas in the Iberian Peninsula (c. 900-1200)' to be submitted under Horizon 2020.

assistant: **Abstract**

As a rule, only those medieval manuscripts that were of interest for the powerful have been preserved. Extant codices were cherished throughout centuries as tangible signs of cultural pre-eminence and carriers of the sacred word; charters were kept as custodians of patrimony and witness to the history of institutions, proclaiming the reasons why monasteries and cathedrals should be revered. But against this rule, other manuscripts that did not directly serve the purposes of high-status minorities were created and are still around. Codices that, belonging to an ecclesiastical rite that was suppressed, should not survive today, but were kept as guardians of tradition. Charters that, intermingled with royal diplomas, have survived without an apparent reason for they do not relate to privileges granted. These sources show us a different side of medieval society in which non-powerful individuals outside central institutions played a crucial role in understanding the implications of written communication, shaping their social memory and that of their past by fully integrating writing in their lives. PeopleAndWriting aims at exploring this common people-writing pairing to uncover what writing meant for lay communities, how it modelled their daily life, and how the use of writing individualised people within their group. From the interdisciplinary study of theses sources that defy the rule, the project proposes a novel method combining approaches from Manuscript Studies to Anthropology to, based upon the graphical, textual, and historical analysis of the sources, reveal the hidden history of the people who wrote, signed, read, and kept handwritten material. Moreover, by focusing on a hitherto unmapped corpus of manuscripts from the Iberian Peninsula, which will be made openly a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Catalytic enantioselective 1,4-addition' will create measurable scientific, societal and economic impact aligned with Fifth Framework.

assistant: **Impact**

The objectives of the present project are the development of highly efficient and enantioselective 1,4- additions of Grignard reagents and other organometallic reagents (RZnX, RLi, RBX_2 and R_3Al) to enones using chiral copper catalysts based on phosphoramidites and related chiral ligands A further goal is to exploit the modular architecture of phosphoramidite ligands to generate also libraries of chiral ligands in order to fine tune the copper/ligand combination for a particular type of organometallic reagent or class of substrates. By screening a variety of phosphoramidites (PN ligands) as well as related phosphites and phosphonates we will select those ligands that combine high turnover with high regioselctivity (1,4- vs 1,2-addition) in the Cu(I)-and Cu(II)-catalysed addition. Small catalyst libraries will be constructed for this purpose. Once suitable active systems have been found, optimization of the enantioselectivity of the copper-ligand combination will be performed. Any major improvement towards enantioselctive catalytic 1,4-addition of Grignards is expected to be highly relevant for the field and if we succeed in reaching the 95% ee level it will undoubtedly be a breakthrough in catalytic asymmetric C-C bond formation with important consequences for organic synthesis.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Smart large lightweight long life Multifunctional PV Module Technology for large Power Installations and Distributed Energy Generation' under Seventh Framework.

assistant: **Concept**

SuMMiT brings value to Europe by demonstration of large lightweight PV modules with record power output, lowest installation cost, lowest PV system cost and lowest carbon footprint in a secure way, i.e. certified performance, quality and durability, protected by patents and proprietary knowhow.SuMMiT combines and demonstrates three step-change innovations: 1. COSMOS PV module design, i.e. a large lightweight hybrid subassembly structure; 2.Highly efficient, long-lasting, top quality, single glass PV laminate; 3.Smart module power management.The demonstration is twofold. The manufacturability of the novel PV module concept will be demonstrated as well as simplicity of installation and utilization, applicability, performance and durability in 5 to 6 field demonstrations.The consortium brings together the required critical mass in polymer technology and polymer processing, encapsulant technology, smart electronics, world class high performance PV module technology and production capacity as well as one of the Europe’s leading turn-key PV system installation companies with subsidiaries all over the world.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'EUROPEAN RAILWAYS OPTIMISATION PLANNING ENVIRONMENT-TELEMATIC RAILWAYS INFORMATION SYSTEM' in the context of a Fourth Framework call.

assistant: **Objectives**

EUROP-TRIS aims to develop a telematics and software system to support expanding national and trans-European traffic planning needs, focusing on long-term tactical timetabling as well as daily or weekly contingency planning.The project plans to automate the traditional conferences at national and European levels and to optimize the utilisation of the railway infrastructure, in line with the new EU policies for the railway transport market. A further aim is to improve operational planning at traffic coordination centres and their communications with intermodal freight links.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Improved assessment of steel buildings performance during earthquakes.' in the context of a Fourth Framework call.

assistant: **Objectives**

The project concerns the analysis of the behaviour of civil engineering steel structures, of the moment resisting category, under earthquake loads. The objective is to implement in such an analysis a quantitative reference to the failure of connections in terms of low cycle fatigue, considering the duration of the earthquake and the corresponding number of cycles supported in dissipative (plastic) zones.The principal activities of the project are as follows:  Full size testing of beam to column connections.  Eight typologies of beam to column connections have been identified as covering a wide range of common  structural applications in steel construction and will be analyzed. Testing on welds.  The problem will be specifically addressed, both for fillet and butt welds, by testing specimens of the two joint  typologies.  Design of the reference steel frame building structure and related numerical analyses.  A two-story industrial/office building will be designed. A preliminary linear-elastic design stress-analysis will  be carried out according to Eurocode 3 (Steel structures) and Eurocode 8 (Constructions in seismic zones)  considering the eight typlologies of beam to column connections previously tested. A numerical non-linear  analysis under the effect of the earthquake will be performed considering four beam to column connections  typologies selected among the eight tested. Finally, out of the four connections experimentally investigated and  numerically examined through the non-linear analysis, one will be selected as of special interest.  Pseudo-dynamic testing of a steel frame building structure.  A one bay prototype of the frame, incorporating the selected connection, will be built and tested to evaluate the  earthquake induced behaviour of the building. The sei
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Integrated Nitrogen Studies in Africa' (INSA), addressing dissemination and exploitation.

assistant: **Impact**

The overall objective of the INSA (Integrated Nitrogen Studies in Africa) project is to work on different aspects of the nitrogen budget in Africa, with interdisciplinary and multidisciplinary competences, based on a network of scientists from Europe and Africa. An essential output of this project will be to create an interoperable platform on N topics to a large public including academics and stakeholders, and to increase the awareness of challenges, risks and chances for a better N management in Africa.According to the “Melbourne Declaration on Responsible Nitrogen Management for a Sustainable Future” (December 2016), “Nitrogen management should strive to simultaneously improve the efficiency of nitrogen use, increase farm productivity, enhance soil health, conserve resources and reduce losses of reactive nitrogen to the environment”. The INSA network will bring its contribution to this great subject of study, and participate to drive the development of a preliminary N assessment in Africa. Many international networks of scientists already exist between countries inside and outside Africa. However, for the first time, it is proposed to link these activities in a large and sustainable network to enhance high quality and multidisciplinary research focused on nitrogen in Africa. This network will gather scientists from Europe and Africa working on ecology, atmospheric chemistry, water quality, soil science, biogeochemistry, agriculture and livestock. The interdisciplinary competences of the groups involved in INSA will help in developing innovative aspects of research on targeted questions, to sustain a dialogue between different communities of researchers, to transfer information to the public and to reinforce educational activities. Education and information sharing wi