RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Fifth Framework proposal on 'Metallo-b-lactamases as model zn enzymes'.

assistant: **Concept**

A full understanding of the structure-activity relationship in proteins is becoming a major challenge of the post-genomic area. Solving this problem requires multidisciplinary studies in which the functional characteristics of a protein are analyzed and correlated with its structural features. Young scientists are needed who can master various subsets of the large number of techniques involved in this approach. Metallo-Beta-lactamases offer a nearly ideal object for this type of research. They constitute a rather diverse family of enzymes with only a very small number of residues strictly conserved in all proteins but the known 3-D structures are clearly related. Similarly, very different specificity profiles have been observed. Depending upon the enzyme, the most active form contains one or two Zn ^++ ions. These can be replaced by other transition metal divalent cations (Co^++, Cd), with concomitant modifications of the activity profiles. The projected studies will rest on the most up-to-date techniques for the analysis of the protein-metal, protein-substrate and protein-inhibitor interaction and for structural analyses. In consequence, significant technological improvement can be expected and it will be possible to apply this new knowledge to other Zn-hydrolases, some of which are of great biological and clinical interest, for instance the matrix metalloproteases. Finally, the emergence of metallo-Beta-lactamase-producing pathogenic strains represents a worrying clinical problem because they can hydrolyze carbapenems, often utilized as last-resort antibiotics and escape the action of the in activators designed against the active-site-serine Beta- lactamases. The genes encoding several MBLs are plasmid or transposon-borne, which makes their spreading by selective pres
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'The Filmmaker in Brazilian Indigenous Communities: the impacts of a new social and political position' in the context of a Horizon Europe call.

assistant: **Objectives**

This project combines approaches from anthropology and film studies to investigate how the systematic use of audiovisual equipment and techniques has contributed to the creation and consolidation of the position of ‘the filmmaker’ in Brazilian indigenous communities. Designed in collaboration with indigenous filmmakers, it will study three communities from different parts of Brazil, each with different histories and traditions of filmmaking - the Kuikuro, the Maxakali, and the Guajajara. In undertaking these studies, BFIC proposes an expanded and comparative analysis of the filmmaker's consolidation as a new social-political position that holds great prestige alongside traditional roles like the community leader, the health agent, the shaman, and the teacher. Around 90% of Brazilian indigenous communities now have their own filmmaker. They are responsible for transmitting technical audiovisual knowledge to younger generations; for cultural registration; and for disseminating information to mainstream media. However, it is also undeniable that indigenous production has gone beyond the limits of ‘ethnographic film’ and occupies a place in wider cinematographic culture. The filmmaker is therefore central to the exchange of symbolic production, and their work contributes to subverting and decolonising commonly held visual notions of what it is to be indigenous. The open access journal articles and collaborative short film produced by this project will explore the consolidation of the position of ‘filmmaker’ in detail for the first time. Alongside a range of dissemination activities targeted at academics and an interested general public, it will also hold audiovisual workshops in indigenous villages to directly strengthen ties between the Kuikuro, Maxakali and Guajajara comm
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Mediators in plant pathogenic fungal-bacterial symbiosis' (FUNBIOSIS) targeting Horizon 2020.

assistant: **Abstract**

Interspecies interactions between fungi and bacteria are highly prevalent in nature and are critically important in a variety of fields including agriculture, food processing, biotechnology, medicine, and dentistry. The study of such bacterial-fungal interactions (BFIs) can provide invaluable information regarding host-pathogen interactions that can be leveraged in understanding and treating important animal and plant diseases. The unique experimental tractability of the Rhizopus-Burkholderia endosymbiosis provides a model system for the investigation of the secreted factors central to BFIs. One remarkable feature in BFIs is the delivery of bacterial effector molecules that can take control of their eukaryotic host cell. FUNBIOSIS aims to speed up the discovery of natural compounds utilised by endosymbiotic Burkholderia sp. to control its fungal host by integrating the emerging technology of microfluidics. We will focus on novel effector molecules that: (i) can modulate host gene transcription (transcription activator-like effectors, TALEs); (ii) are associated with the recently discovered type 6 secretion system (T6SS); and (iii) are involved in chromatin-dependent changes in the host genome. This will not only elucidate the nature of host control in this commercially important BFI but also has the potential to serve as a roadmap to understanding how prokaryotic pathogens and symbionts interact with more complex eukaryotic hosts. Understanding the biology, ecology, and biotrophic interactions of the zygomycetes (of which Rhizopus microsporus is a member) with their associated endobacteria can help to identify novel secondary metabolites which are central to ecological functions and are useful for effective and innovative biotechnological utilisations.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'MARGINAL GAS FIELD DEVELOPMENT - THE RE-USE OF SUBMARINE PIPELINES' (1994-1998).

assistant: **Innovation**

The aim is to re-use existing submarine pipelines during the development of a marginal gas discovery. The existing pipeline will be located, cleared of sand cover, recovered and re-routed to a new location and used for the transport of gas from the new marginal gas field. All subsea operations will be conducted with the use of an ROV in order to make the project diverless, a requirement for operations in deeper waters of the Northern North Sea.The principle aim of the project is to develop the necessary technology and methods in one specific but vital aspect of developing small accumulations of oil and gas discoveries, generally termed as Marginal Fields.The exploitation of marginal gas discoveries is already becoming vital to the continuity of the supply of natural gas in particular. This project will help make exploitation of such fields economic in the current and near future economic climate. A secondary aim is a positive contribution to the environment by recycling material instead of creating new and removal quantities of unused metal from the marine environment.Preliminary engineering has been carried out to assess the feasibility of the project, this study has indicated that it will be possible to carry out the project with the assistance of newly developed ROV interfaced equipment and systems. No further results are available for publication at the present time.Full engineering has now been completed in order to carry out a full scale test on a section of unused pipeline. A scale test of the of the Delta Wing System was performed in the Delft University Test Tank.Expected Pipe stress analysis due to wave actions have been carried out.The size of Oil and Gas discoveries in the North Sea is decreasing, prompting a search for innovative methods and technologies to
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Smart photo-activable devices based in plasmonic nanoparticles: Microfluidic-assisted engineering of a third generation of lipid vesicles' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

Giant lipid vesicles can be potentially used as biocompatible carriers with a large lumen cavity adequate for lodging large biomacromolecules in an aqueous compartment. New developments in controlled delivery are strongly troubled by the high polydispersity of the preparations and the limitations in the encapsulation abilities inherent to conventional preparative methods. Engineering smart vesicles with tunable and remotely controllable properties such as permeability, osmotic deformability or inducible instability, necessary for adequate delivery, is indeed a major synthetic challenge. This implies a number of basic operations, which include bilayer assembly, composite membrane stabilization, encapsulation and compartmentalization, a set of procedures requiring a novel approach. For this performance, we propose the use of microfluidic technology and high-speed imagining to design and study the active response to photo-irradiation of smart giant unilamellar lipid vesicles with plasmonic gold nanoparticles embedded in the membrane. Excitation of the surface plasmons of the nanoparticles produces localized heating of the membrane, thus controllable changes in permeability, which could eventually result in an enhanced osmotic-driven flow of solvent across the membrane and cause an overall change in size and shape of the entire vesicle. Using these model systems we will be able to shed light on the physical mechanisms involved in the transference of conformational- to mechanical- energy, which could be relevant to a broad range of scientific problems ranging from the fundamental knowledge in cell biology, concerned by the study of cellular functions such as endo- and exocytosis and cell motility, to applications in drug delivery and material engineering, both enrolled in th
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Japan-EU Partnership in Innovation, Science and TEchnology' goes beyond current state-of-the-art under Seventh Framework.

assistant: **Innovation**

The JEUPISTE project aims at promoting EU-Japan cooperation in Science, Technology and Innovation (STI) through supports to policy dialogues, deployment of bilateral information services, organization of networking events focusing on specific technologies and/or societal challenges, operation of a help desk for EU-Japan collaborative projects (including advice and assistance in administration, liaison with the EC and Japanese funding organizations, partner search etc.) and contribution to the development of human resources for collaborative projects.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Development of sustainable selective catalytic oxidation of alkanes', explaining its relevance to EU policy priorities under Seventh Framework.

assistant: **Impact**

Saturated hydrocarbons are chemically inert and therefore their functionalisation is challenging from a basic science viewpoint. The development of selective, energy efficient direct alkane oxidation chemistry could lead to environmentally and economically superior chemical processes and allow the vast reserves of natural gas and petrol to be used more efficiently and economically as feedstocks for fuels and chemical products. Like the active centres of some enzymes, transition metal complexes can potentially activate and functionalise the non-activated C-H bonds of hydrocarbons with high atom economy and low energy under mild reaction conditions. There is also an urgent need to increase the sustainability of homogeneous phase reactions and to develop practical, efficient and low energy immobilisation methods for homogeneous catalysts, as well as to develop and use new, cheaper and more environmentally acceptable solid supports. This project comprises the synthesis and characterisation of transition metal coordination compounds with very versatile and biomimetic polydentate ligands and their application as selective homogeneous catalysts in the oxidation reactions of alkanes under mild conditions and using several oxygen sources. The use of a microwave technology to carry out these reactions could also lead to increases in product yields and selectivities, as well as being more energy-efficient compared to conventional heating methods. To improve the sustainability of these homogeneous catalysts in the catalytic oxidation of alkanes several porous silica and carbonaceous materials will be used as catalysts supports. The use of microwave-activated loading onto these supports will be also tested as a more energy-efficient and a more selective technique than more conventio
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Screening of small molecules to fight SARS-CoV-2 by combining novel approaches and sustainability' (SPOTLESS).

assistant: **Concept**

The novel SARS-CoV-2 is a lethal human pathogen with no FDA approved vaccines or drugs. An important step of the virus life-cycle is the viral glycoprotein S activation by Proprotein Convertases (PCs) which are proteases with a broad spectrum of cellular substrates. Inhibition of the processing step locks the virus into a form which eventually is no more pathogenic. Therefore, PCs represent attractive drug targets to fight against SARS-CoV-2. To date, there are no PCs inhibitors available for the in vivo use. Here, I propose three approaches to look for small molecule inhibitors of PCs, exploiting the virus cleavage site. Beside the classical in vitro screening, I aim to exploit novel PCs sensor platforms based on innovative ideas to ameliorate and simplify the readout. One attempt will see the use of luciferase reporters on the blueprint of sensors that I have recently published; following a second method, I will take advantage of the cellular death machinery as an on/off cleavage signal. In turn, these virtuous assays will contribute to a sustainable research approach. Novel PCs inhibitors are of great value against Covid-19 infections. Importantly, the inhibitors can be re-purposed against novel emerging pathogens, if necessary. Indeed, PCs dependence is becoming a distinctive mark of severe pathogenicity.


**Keywords:** SARS-CoV-2,  COVID-19,  Proprotein Convertases,  Screening,  HTS,  inhibitors,  cleavage
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Cell-free synthetic biology for combinatorial biosensor design' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

Recent advances in the field of synthetic biology offer an unprecedented opportunity for next-generation biosensors characterized by programmability, low-cost and by a sensitivity and specificity unmet by conventional sensors. Leveraging the rich toolset of synthetic biology, we can now not only build better biosensors but also analytical tools that can sense and discern complex analyte profiles, such as a panel of biomarkers indicative of a certain disease status. These cell-free biosensors developed within the SYNSENSO research program will be transformative for many domains of research and innovation, ranging from in-vitro diagnostics to health, environmental and bioprocess monitoring. Our ambition is to provide a game change in biosensing by combining cell-free synthetic biology with molecular sensor design. The SYNSENSO European doctoral network focuses on the construction of novel programmable ligand-responsive elements and the construction of cell-free computing circuits that can integrate molecular signals from those elements. Together the two components will make up the next generation of combinatorial biosensors that can sense signals and compute a response to them. SYNSENSO aims for a modular approach where different responsive elements can be composed with different computing circuits enabling fast construction of suitable sensors for new application domains. SYNSENSO brings together academic and industry leaders from the fields of synthetic biology, molecular sensor design and DNA nanotechnology. Our industry partners will provide a unique ecosystem for ten doctoral candidates of this network. Together with the external advisory board including experts from medical diagnostics they will work on concrete case studies for clinically relevant analytes. The SYN
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon 2020 proposal on 'Sacralizing Security: Religion, Violence and Authority in Mega-Cities of the Global South'.

assistant: **Concept**

In mega-cities of the Global South, state agencies often lack the capacity to provide infrastructure and security to all citizens. In such contexts, religious organizations and non-state security actors (vigilantes) have merged into alternative governance organizations.The emergence of religious vigilantes suggests a different connection between religion and violence than emphasized in current research on religious fundamentalism and terrorism. While religious vigilantes use violence systematically, they generally do not aim to overthrow the state, nor do they seek a global audience to witness their violence. They operate side-by-side with state actors to maintain order. Major questions are: why do mega-city residents grant these religious vigilantes authority? And what is the role of religion in the legitimation of vigilante practices?SACRASEC will analyze the production of authority of religious vigilantes in mega-cities of the Global South through an ethnographic comparison of three mega-cities. The case studies focus on Christian and Afro-Brazilian religion in Rio de Janeiro; Christian, Islamic and Indigenous religion in Lagos; and Islamic and Indigenous religion in Jakarta. This comparative focus makes it possible to draw general conclusions about the role of religion in alternative governance, while also enabling an analysis of the particularities of each religious tradition in the context of vigilantism.This research extends the boundaries of the anthropology of religion and political anthropology. It will provide critically needed knowledge on the power structures in mega-cities of the Global South, and in so doing will contribute important insights to policies aimed at improving human security.


**Keywords:** Security,Alternative Governance, Vigilantism
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'HYDROGEN MEETING FUTURE NEEDS OF LOW CARBON MANUFACTURING VALUE CHAINS' (H2Future).

assistant: **Concept**

Under the coordination of VERBUND, VOESTALPINE, a steel manufacturer, and SIEMENS, a PEM electrolyser manufacturer, propose a 26 month demonstration of the 6MW electrolysis power plant installed at the VOESTALPINE LINZ plant (Austria). After pilot plant commissioning, the electrolyser is prequalified with the support of APG, the transmission operator of Austria, in order to provide grid-balancing services such as primary, secondary or tertiary reserves while utilising the commercial pools of VERBUND. The demonstration is split into five pilot tests and the quasi-commercial operation to show that the PEM electrolyser is able both to use timely power price opportunities (in order to provide affordable hydrogen for current uses of the steel making processes), and to attract extra revenues from grid services which improves the hydrogen price attractiveness from a two-carrier utility like VERBUND.  Replicability of the experimental results at larger scales in EU28 for the steel industry (with inputs from TSOs in Italy, Spain and the Netherlands) is studied under the coordination of ECN. It involves a technical, economic and environmental assessment of the experimental results using the CertifHY tools.  The roll out of each result is provided by ECN, together with policy and regulatory recommendations to accelerate the deployment in the steel and fertilizer industry, with low CO2 hydrogen streams provided also by electrolysing units using renewable electricity. The plausibility of this roadmap is reinforced at the on-start of the demonstration by the creation of an exploitation company involving the core industrial partners, which starts commercial operations of the Linz pilot plant right after the end of the demonstration. Dissemination targeting the European stakeholders of