RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Elucidation of risk factors and health determinants associated with PROGRESSION of Monoclonal Gammopathies to Multiple Myeloma', explaining its relevance to EU policy priorities under Horizon Europe.

assistant: **Impact**

Multiple Myeloma is a chronic malignancy characterized by slow progression and recurrences. Currently there is no effective cure since eventually the disease develops resistance to all the available therapeutic approaches. Although recent advances have expanded our understanding of the cellular functions associated with health to disease transition, recurrence and response to therapy, critical aspects of this complex pathology remain to be elucidated. Application of omics technologies, and bioinformatics approaches on highly annotated samples obtained from all informative states (monoclonal gammopathy of undetermined significance [MGUS], smoldering MM [sMM], active MM [MM]) could identify biological pathways and molecules responsible for the onset, progression and resistance to therapy of Multiple Myeloma. In parallel, particular emphasis will be given to elucidating the health determinants and risk factors associated with progression to active MM from MGUS/sMM by using extensive demographic, lifestyle and exposure datasets. MGUS is present in 3-5% of the ageing European population and every year, 1% progress to incurable MM that imposes a significant burden on EU societies and health systems. Thus, the best chances of curing MM may be in preventing its progression in the first place. Moreover, there is need of experimental models that recapitulate myeloma progression.We propose an interdisciplinary approach bringing together clinicians and researchers aiming to integrate epidemiological, clinical and experimental datasets in order to create a molecular model of cellular processes associated with the onset of active MM and response to therapy. The proposed systems medicine approach could yield clinically actionable molecular features that could improve MM patient manage
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon 2020 proposal on 'Neural mechanism underlying vocal interactions in duetting nightingales'.

assistant: **Innovation**

Humans and many animals produce complex vocal sequences in order to communicate with each other. What are the neuronal mechanisms that integrate the auditory information and permit the production of a coordinated motor response during a vocal interaction? I will address this issue in the nightingale, a songbird species that is capable of duetting with rivals. During vocal interactions, nightingales have to precisely alternate between singing and listening, and I will test whether premotor centers are differentially sensitive to auditory input during these two states. In zebra finches, I have shown that the impact of a father’s song on premotor circuitry can be regulated by inhibitory interneurons during developmental song learning. Here I ask whether this same regulation of auditory input can rapidly change to support real-time vocal coordination in a duetting songbird.To measure neuronal activity during listening and singing, I will use intracellular recordings to assay the synaptic inputs and outputs of a premotor circuit. I will use a motorized intracellular microdrive that I helped to develop during my postdoc in order to enable these measurements in the freely behaving bird. A custom built vocal robot will be used to dynamically interact with birds during neural recordings. These experiments will reveal the synaptic profile of neurons during sensorimotor integration and clarify how nightingales are able to sing a temporally precise duet. The aims of my research proposal are 1) to investigate how auditory input influences the motor program, 2) how this auditory input is gated depending on behavioral demands and 3) how a song motif is generated on a neuronal population level. I will elucidate neural dynamics essential for vocal interactions, which may provide insight
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Cardio Ultraefficient nanoParticles for Inhalation of Drug prOducts' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

The incidence of Cardiovascular Disease (CD) claims worldwide 17.1 million lives a year, with an estimated 31% of all deaths globally and a EU cost of 139 billion euros. Up to 40% of all deaths occur among the elderly. In spite of all medical efforts, the 5-year mortality was reduced significantly less than that of malignant diseases. This highlights the urgent need to overcome the difficulties associated with present pharmacological therapies (i.e. drug instability, and unspecific targeting) by developing new ground-breaking therapeutic strategies that go far beyond any current regimens. New approaches for safe, efficient, and heart-specific delivery of therapeutics are strongly required. CUPIDO is envisioned to meet these critical needs by providing an unconventional and effective strategy based on nanoparticle-assisted delivery of clinically available and novel therapeutics to the diseased heart. In particular, CUPIDO will develop innovative bioinspired hybrid nanoparticles formulated as biologicals delivery, which are i) biocompatible and biodegradable, ii) designed for crossing biological barriers, and iii) guidable to the heart. A combination of multidisciplinary manufacturing and validation approaches will be employed, bringing the envisioned product beyond the currently available clinical and day-to-day management of CD individuals. Scale-up production,  and respect of medical regulatory requirements will allow CUPIDO to deliver a final product for future late pre-clinical and clinical studies. Altogether, CUPIDO will foster the translation of nanomedical applications toward the cardiac field, which although still in its start, offers great potential to overcome the limitations associated to the currently pharmacological treatments.


**Keywords:** Cardiovascula
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Ribosome dynamics analysed by novel cross-linking/mass spectrometry' (RSDyn) targeting Seventh Framework.

assistant: **Abstract**

Ribosomes, large and dynamic particles made of RNA and proteins, are amongst the most complex macromolecular machines known to man. High resolution tools have provided exciting views of the ribosome structure. Revealing dynamics of ribosomal complexes in solution, however, has so far been challenging due to the restricted stability and homogeneity of ribosomes. A set of new tools developed by the Rappsilber lab, partly by me over the last few months, changes dramatically the feasibility of investigating ribosome dynamics. I aim to use these new mass spectrometry-based technologies to determine accurate sites and changes in yield of cross-linking between proteins and between RNA and proteins. Thereby, using protein-protein and RNA-protein cross-linking/mass spectrometry approaches, I aim to answer:1.Is the final and rate-limiting step in the biogenesis of 50S ribosomal subunits a conformational rearrangement? This will prepare the subsequent analysis of the other steps during the biogenesis of ribosomes in E. coli and in future also in the eukaryote S. cerevisiae.2.How does the structure and extra-ribosomal protein content of translating ribosomes change in response to different stress conditions? This will complement electron microscopy data and lead into the analysis of how translation links to virulence in pathogenic bacteria.My project will showcase the potential of cross-linking/mass spectrometry for the study of ribonucleoprotein complexes and, indeed, for protein-nucleotide interactions in general, i.e. encompass DNA-protein interactions. Furthermore, results of this study may facilitate the development of next-generation antibacterial drugs, targeting assembly factors and conformational rearrangements during ribosome biogenesis. For my academic career, acquiring
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Unjust Enrichment and Public Policy' (UEPP).

assistant: **Concept**

The basic maxim of the law of unjust enrichment states that a person should not be unjustly enriched at anothers expense. This broad principle is currently primarily used to solve local problems and disputes between specific individuals through private litigation. The proposed project offers a paradigm shift and the use of the fundamental principle prohibiting unjust enrichment to solve broad societal issues, both as a private and a public law doctrine. This is a double paradigm shift. From the direction of the law of unjust enrichment, it requires a reconceptualization of this area of law, to adjust it to solve entirely new types of problems. From the direction of public law, it offers to refresh the way we currently look at deep societal ills such as global warming, the spread of fake news or the production of harmful goods and services. In all these contexts, and many others, current legal frameworks tend to focus on the harmfulness of certain activities. The proposed framework offers, instead, to start the analysis with undeserved and ill-obtained gains. Phenomena like global warming and fake news are problematic because they are harmful, but persistent, and so difficult to regulate, because they are immensely beneficial to strong interest groups. A regulatory perspective focusing on gains can therefore prove instrumental in fighting some of the most important legal battles of our time. This paradigm shift involves a broad project and requires a multistage research program, conducted by the PI together with a team of post-doctoral researchers. This is a high-risk high reward project; if successful, it can provide critically missing elements in the legal toolkit, that will improve our ability to solve pressing legal issues.


**Keywords:** private law, public law, re
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Fifth Framework proposal for the project 'Innovative GALlium Arsenide MMICs and Advanced LTCC Packaging Technologies for Multicomponent Ka-band Subsystems'.

assistant: **Objectives**

GALACTIC will develop innovative key technologies for affordable multicomponent Ka-band subsystems. These technology building blocks are: low cost, highly integrated GaAs MMICs and LTCC technologies for critical millimeter-wave components and power chip insertion. They will be integrated and validated in the next generation Ka-band VSAT transmitter, which is required for various new products in next generation satellite broadband communication networks. Through GALACTIC, these building blocks will become affordable, reliable and adequate for mass production. Many future wireless communication products and networks will thus have their prices substantially reduced and the viability of their fabrication process will be ensured.Objectives:GALACTIC will develop innovative key technologies for high performance, affordable, multi-component Ka-band subsystems. The objective of the GALACTIC project is to lay the foundations to achieve this goal. The key innovative technologies are compact multifunctional circuits (MFCs) based on integrated Gallium Arsenide (GaAs) monolithic microwave integrated circuits (MMICs), low temperature co-fired ceramics (LTCC) for high-power Ka-band applications, their integration and packaging. Those technologies are required for the design, fabrication and validation of a low cost, multi-component high-power Ka subsystem adequate for high volume production. They will be integrated and validated on a Ka-band up-converter transmitter, which is the key building block of various future products for next generation broadband access systems.Work description:The consortium will develop the following technologies required to produce the multi-component Ka-band subsystems:- Low cost LTCC technology for compact and low loss millimeter-wave circuitry e.g.transi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Unraveling Brassinosteroid Biosynthesis: Orchestrating BR Precursor Movement via Endoplasmic Reticulum-Plasma Membrane Contact Sites (EPCSs)' to be submitted under Horizon Europe.

assistant: **Abstract**

Since their discovery in 1979, brassinosteroids (BRs) have been extensively studied for their biosynthesis and signalling pathways. Now, in the year 2023, one fundamental question remains: how do BRs move? As the only type of steroidal phytohormones essential for plant growth and development, BRs act in a dose-dependent manner and do not travel long distances. Therefore, BR homeostasis is key to their function. Recent findings in my host lab revealed that in the Arabidopsis root the expression domains of BR biosynthetic enzymes are separated, and the completion of BR biosynthesis requires cell-to-cell movement of precursors, thus, revealing the importance of the short-distance transport for BR homeostasis maintenance. Further we revealed that plasmodesmata (PD) mediate the passage of BRs between neighbouring cells. However, we still do not know where in the cell BRs are synthesized and how these hydrophobic molecules are moving through PD. By searching interactors of BR biosynthetic enzymes, my host lab found interesting candidates associated with the endoplasmic reticulum (ER)-plasma membrane (PM) contact sites (EPCSs), including PD. EPCSs are known lipid synthesis platforms and contribute to PD function in plants. Here, I hypothesize that BRs are synthesized at EPCSs, and EPCSs coordinate BR biosynthesis and transport through PD. In BRACTION, I will combine live cell imaging with state-of-the-art imaging techniques to pinpoint BR biosynthesis sites in the cell. Combining advanced proteomics, genetics, and biochemistry, I aim to identify novel candidates that regulate BR biosynthesis and movement linked to EPCSs. Finally, I will employ genetics combined with bioorthogonal chemistry approach to assess candidate influence on BR symplastic movement. BRACTION's interdiscip
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Study of polyketide synthase structure' will create measurable scientific, societal and economic impact aligned with Fourth Framework.

assistant: **Impact**

Research objectives and content The aim of this study is a better unterstanding of the biosynthesis of polyketides, especially of formation the lacton ring. Therefore we will overproduce several essential polyketide synthase domains, mainly a acetyl transferase, a Beta-ketoacyl synthase, an acyl transferase and an acyl carrier protein, in E. coli and / or Saccharopolyspora erythrea. The recombinant proteins will be purified and examined for their catalytic activities. Therefore we will investigate the modification of ACP with the cofactor 4'-phosphopantetheine. This will allow the development of new enzymatic assays for exploring the catalytic activties of the Beta-ketoacyl synthase domain. Furthermore, this could be a first step towards a complete in vitro biosynthesis of macrolactone rings. Another aspect will be the crystallization of the domains in order to obtain precious insights into the three-dimensional structure. These findings will allow to determine, which aa-residues are important for catalytic activity or interactions with other domains. Subsequently, it would be possible to point out the functions of these aa-residues by mutational analysis. Training content (objective, benefit and expected impact) After investigating the non-ribosomal biosynthesis of peptide-antibiotics, it will be very interesting for me to extend this knowledge to a new challenging field of antibiotic-research. Producing polyketide synthase domains domains and investigating the catalytic activities of these recombinant proteins will offer me a good opportunity to gain experience with Actinomycetes such as Streptomyces and Saccharopolyspora and the enzymology of polyketide biosynthesis. Links with industry / industrial relevance As far as this study is concerned, there will be no direct
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'The utilisation of waste leather as a co-raw material in cellulose board manufacture' to be submitted under Fourth Framework.

assistant: **Abstract**

Boards are used in a number of industries, they normally consist of fibres or particles pressed together, with or without, a binder to form a continuous matrix in a sheet form. The properties of the board vary depending on the constituents and the processes used and range from rigid to flexible materials of differing strength and durability. Many of the boards used are cellulose based these being a common choice on the basis of price, appearance and properties. However there is scope to further improve the quality of these boards for current applications and also to extend their range of use which is currently limited in some applications, e.g. moulded items for automotive applications, by the properties of the cellulose fibre. Therefore if a co-raw material can be incorporated which extends the property range of the boards there is scope for greater usage of these products. There is currently an added incentive to finding a co-raw material in that the price of the raw material for the cellulose board, wood pulp has increased dramatically and this trend looks set to continue. Leather has greater strength, flexibility and durability than most cellulose products and it is known that board products can be produced from pulverised leather waste. However to maintain the advantages of the leather in a mixed fibre board the leather incorporated would need to be in a long fibre form otherwire the leather would simply act as a filler for the cellulose. Currently no process is available which can convert leather into a suitable long fibre form. The proposed project therefore aims to develop a process which can utilise both leather and cellulose fibres as raw materials, design and build prototype equipment for leather fibre preparation and develop a mixed fibre board of higher qua
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Cellular and structural determinants of amyloid toxicity' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

Neurodegenerative disorders such as Alzehimer´s or Parkinson´s affect millions of people in Europe and are linked to the misfolding and aggregation of proteins in the form of amyloid fibrils. The molecular pathways of amyloid aggregation are complex and poorly understood, and involve the population of oligomeric intermediates before fibrils form. Little is known about the role in vivo of amyloid intermediates, despite generally recognized as the major neurotoxic agents. The challenge of this project is to bridge the gap between structural and functional studies of amyloid intermediates, and to rationalize the role of protein-associated factors in amyloid toxicity. I intend to provide new experimental evidence into the causes of conformation-dependent neurotoxicity for the protein alpha-synuclein, the amyloid culprit of Parkinson’s disease. I will identify cellular factors associated with amyloid intermediates by employing mass spectrometry techniques. A protein interaction map for such amyloid species will be created based on the proteins identified. This protein network will provide a pathological atlas of the cellular pathways affected upon amyloid formation. Selected protein targets of the oligomeric intermediates will be characterized structurally, with the aim of identifying structural determinants of amyloid toxicity, the so-called amyloid hot spots. State of the art biophysical methodologies, such as NMR, fluorescence spectroscopy and microscopy will be employed, all of them available at the host institution. Finally, computational modeling of protein-protein interactions will support the experimental work, another major expertise of the host laboratory. It is foreseen that such amyloid hot spots will naturally become targets for therapeutic intervention. Accordi