RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon Europe proposal on 'Conjugative-killer plasmids, a novel antimicrobial alternative'.

assistant: **Concept**

Targeted killing of pathogenic bacteria without harming beneficial members of the host microbiota holds promise as a strategy to cure disease and limit both imbalances in the microbiota and development of antimicrobial resistance. Recent work from the Unité de Plasticité du Génome Bactérien has demonstrated that genetic modules based on toxin-intein systems delivered by conjugation are highly effective antimicrobials agents, able to selectively kill Vibrio cholerae in mixed populations. In this line of work, the project described in this proposal aims at adapting the aforementioned system to other pathogens of clinical importance (Salmonella spp. Shigella spp and Klebsiella pneumoniae) by including new toxin modules whose expression depends on transcriptional factors that are exclusively present in the targeted bacteria. Additionally, to ensure an efficient dissemination and maintenance across the microbial gut population, we intent to engineer conjugative plasmids to be transferred and maintained between Enterobacteriaceae and Bacteroides, one of the main constituents of the gut microbiome. Once validated under laboratory conditions, the system will be assayed in a Caernorhabditis elegans model, either with the aim of eliminating a specific pathogen causing disease or as a probiotic agent against pathogenic colonization. The results obtained from these preliminary tests will direct the refinements needed for the generation of an effective tool against antimicrobial resistant pathogens in a real scenario.


**Keywords:** Antimicrobial Resistance; Bacterial Toxins; Inteins; Plasmid Biology; Conjugation; Genetic Engineering
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Crystals of single chirality via non-equilibrium routes' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

The molecular building blocks of life are of only one handedness. Consequently, pharmaceuticals and other bioactive molecules also need to be of one handedness. When such enantiomers crystallize in separate crystals, isolation of the desired handedness is relatively straightforward. Unfortunately, most enantiomers (90-95%) are thermodynamically more stable as racemic compounds with both enantiomers in the crystallographic unit cell, which impedes any such separations. There are currently no methods to systematically overcome this major bottleneck, thus hindering simple routes towards many essential enantiopure molecules.  This proposal is aimed at overcoming this fundamental challenge by establishing new principles to turn racemic compounds into molecules of a desired handedness by liberating them from their thermodynamic constraints. To achieve this ambitious goal, we introduce a revolutionary new approach: manipulating the stability of crystals by subjecting mixtures of crystals to non-equilibrium conditions. Building upon our preliminary work, we hypothesize that growth/dissolution rates can be manipulated by selecting crystallization conditions such that the thermodynamically stable racemic compound is converted into the desired enantiomer. To achieve this ambitious goal, the main objectives of this proposal are to: (i) demonstrate the proof-of-principle, (ii) identify the essential parameters, and (iii) understand the mechanism behind this methodology.   The results of this ERC Consolidator will hold direct relevance for our fundamental understanding of non-equilibrium conditions in reactive crystallizations, and the outcomes of this research will immediately impact our ability to produce molecules of single handedness. Ultimately, this breakthrough holds the poten
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Seventh Framework proposal on 'The role of noncoding RNA in sense and antisense or orientation in epigenetic control of rRNA genes'.

assistant: **Concept**

Non-coding RNAs (ncRNAs) play a significant role in the control of gene expression and epigenetic regulation. It seems that ncRNAs might be numerous and highly adapted in roles that require specific nucleic acid recognition without complex catalysis, such as in guiding RNA modifications or in directing post-transcriptional regulation of gene expression and chromatin structure. Our previous work has revealed that NoRC, a chromatin remodeling complex that triggers heterochromatin formation and transcriptional silencing of a fraction of rRNA genes, is associated with 100-250 nt RNAs that originate from the intergenic spacer (IGS) separating rDNA repeats. Furthermore, a fraction of rDNA is transcribed in antisense orientation. Both IGS RNA and antisense transcripts display a growth- and tissue-specific expression pattern. The goal of this project is to decipher the role of NoRC-associated RNA in alterations of chromatin structure and epigenetic control of rDNA. Our research will focus on the synthesis, regulation, and processing of intergenic and antisense transcripts in response to cell growth and differentiation as well as on the role of NoRC-associated RNA in epigenetic regulation of rRNA genes.   The following points will be addressed: (1) Deciphering the mechanism underlying RNA-directed establishment of specific epigenetic marks and formation of silent chromatin domains, (2) Functional analysis of posttranscriptional modifications that regulate RNA binding and NoRC activity, (3) Identification of non-ribosomal target genes of NoRC, and (4) Elucidation of the link between transcriptional activity and active demethylation of the rDNA promoter. Given the fact that basic regulatory principles are conserved throughout evolution, this work will have a great impact on our un
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Nonlinear Interaction of Terahertz Light with Two-Dimensional Nanomaterials' (TeraNanoLIGHT) under Horizon Europe.

assistant: **Objectives**

Two-dimensional (2D) nanomaterials such as graphene and transition metal dichalcogenides (TMDs), hold great promise for the engineering of modern nanophotonic devices that will operate at terahertz (THz) speed rates, with reduced energy requirements. Particularly, their nonlinear properties at THz frequencies, are expected to be the key element for the development of THz nanodevices that can generate new frequencies, control light propagation or act as nonlinear optical modulators. The TeraNanoLIGHT project aims to study with nanometer spatial and femtosecond temporal resolution, the ultrafast interaction of 2D nanomaterials with atomically strong (multi-MV/cm) THz fields, promoting the light-matter interactions into the non-perturbative regime. Intense THz transients will be combined with a scattering-type scanning near-field optical microscope (SNOM) to achieve atomically strong fields. Initially, THz surface plasmons (SPs) will be resonantly coupled in graphene, and their nonlinear behavior will be studied by monitoring their formation, propagation and temporal evolution as the THz field strength increases into the nonlinear regime. Furthermore, non-resonant nonlinearities will be explored, exploiting the oscillating THz field as an ultrafast AC bias. The possibility to observe high harmonic generation (HHG) with characteristics similar to those of atomic gases, will be studied in monolayer TMDs and heterostructures of different layers number and twist angles, excited by an out-of-plane polarized THz field. Finally, the opportunity of exploiting the extremely nonlinear interaction of light with matter, to improve the spatial resolution of SNOM, will be investigated. TeraNanoLIGHT project, envisions to facilitate our understanding about the ultrafast interaction of in
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Robotic self/other distinction for interaction under uncertainty' (SELFCEPTION).

assistant: **Innovation**

There will be around 35 million private or non-industrial use robots in the world by 2018, a market of 19 billion euros. However, autonomous robot technology in Europe is not yet ready to lead this high expectancy due to the lack of robust functionality in uncertain environments. Particularly, safe interaction is an essential requirement. A basic skill, still unachieved, is to allow the robot to be aware of its own body and perceive other agents. Recent evidence suggests that self/other distinction will be a major breakthrough for improving interaction and might be the connection between low-level sensorimotor abilities and conceptual interpretation.Advanced sensorimotor learning combined with new multimodal sensing devices, such as artificial skin, makes now possible that the robot acquires its perceptual representation, and I hypothesize that learning the multisensory-motor spatiotemporal contingencies permits self/other distinction. Hence, the aim of the project is to provide a hierarchical probabilistic model for self/other distinction in robots, learning the sensorimotor contingencies during interaction. This model not only provides a holistic solution for building the perceptual schema and improves interaction under uncertainty but it might also give insights about how humans construct their own perceptual representation and the sense of agency. Finally, the model will be tested in a whole body sensing humanoid and validated in a service robot in collaboration with a robotics SME. I will use an interdisciplinary approach that combines probabilistic and information sciences modelling with cognitive psychology, creating a highly attractive career profile.SELFCEPTION will boost the materialization of the next generation of perceptive robots: multisensory machines abl
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Prion Detection Through Organized Arrays of Gold Nanorods as SERS Substrates' (PRIOSERS).

assistant: **Concept**

This proposal aims the design of novel optical enhancers for surface-enhanced Raman scattering (SERS) to develop routine methods for quantitative detection of a very broad range of substances based on regular arrays of gold nanorods. As proof of concept the diagnosis of neurodegenerative diseases, such as Alzheimer or Creutzfeldt-Jakob diseases (CJD), which still pose a great challenge for international health systems because of the economic and social impact of its pandemic outbreaks. Such diseases do not induce any immunological response on the infected individuals and thus, antibody detection cannot be used because they are not produced by the host. Therefore, antibody-free detection systems are required, which are also highly sensitive and selective. Overall this presents a significant challenge which can be resolved using a new generation of SERS substrates with unprecedented degree of structural control.Therefore, the main objectives of the project will involve the fabrication of uniform gold nanorods within a wide range of sizes; their assembly into perfectly ordered supercrystals, both on planar and patterned substrates; evaluation of the SERS enhancing properties of such assemblies as a function of nanorod morphology and degree of order; comparison with theoretical modeling and prediction of the most convenient configuration; and finally the full implementation of the detection of prions using these substrates.  We propose the use of SERS as a non-invasive sensor, which is able to detect and monitor prions in biological fluids (blood, urine or saliva). For this target to be achieved, the enhancing metallic substrates need to be engineered for focalization of the plasmonic modes at certain regions of the substrate and production of extremely high enhancement fac
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Development of new cutting technology for production of construction materials - bricks, tiles' goes beyond current state-of-the-art under Fourth Framework.

assistant: **Innovation**

SHAPECUT Development of a new cutting technology for the production of advanced ceramic construction materials (bricks, tiles, . .) Industrial needs. The cutting systems of brick and tile manufactures are placed at the output of extrusion in the manufacturing process and are used to cut pasty clay. The technical evolution of these lowperformance tools can go no further. Industrialists are consequently looking for a solution which will enable them to improve cutting quality while increasing productivity, but, most of all, which will meet the new needs of professionals of the building trade. The current trend is, in fact, advanced products used, for instance, for male-female interlocking.Professionals are willing to invest immediately in a new system capable of executing these new complex cutting shapes. Oriqin and structure of the partnership. The project was instigated by NEIFFEL Technologies in Toulouse, the rose red" city in the South West of France where brick remains the prime building material. An analysis of the needs of industrialists in Mediterranean countries (Portugal, Greece, Italy and France) has revealed the need for these small- and medium-sized companies to conduct research into new cutting systems. This technological progress will, in addition to the durability of these companies. ensure their immediate development. A machine tool manufacturer in a highly underdeveloped rural area who, as a result of the massive drop in orders from his main customers (civil aviation, military and shipbuilding sectors), wishes to diversify his markets, is a participant in the program. The research experience of the Mechanics/Physics Materials Department of the Ecole Centrale in Lyons, the know-how of the Greek CERECO and Portuguese CTCV research centres specialized in cer
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Charting the multi-TeV cosmos: long-range interactions in dark matter and baryogenesis' goes beyond current state-of-the-art under Horizon 2020.

assistant: **Innovation**

The origin of the matter-antimatter asymmetry of the universe and the nature of dark matter are among the most fundamental and challenging questions in physics. Their undeniable importance has placed them in the forefront of the experimental and theoretical research in particle physics, cosmology and astrophysics. Our experimental probes are now at the outset of exploring the multi-TeV energy scale. To fully exploit the experimental effort, to design effective search strategies and correctly interpret the experimental results, we must develop reliable theoretical understanding of the plausible dynamics at this scale.The TeV scale is a new threshold. In this regime, the interactions hypothesised in a variety of well-motivated theories manifest as long-range. This is true for the most widely studied particle-physics scenario for dark matter, particles coupled to the Weak interactions of the Standard Model, as well as many other models. Moreover, many theories of matter-antimatter asymmetry generation invoke heavy particles that couple to lighter force mediators.Long-range interactions imply very different dynamics than the contact-type interactions most commonly considered in the past. They give rise to non-perturbative effects, with the most prominent being the existence of bound states. Such effects can change the experimental signatures very significantly. CosmoChart will comprehensively investigate the implications of long-range interactions along two directions: I.  The dark matter thermal decoupling in the early universe and indirect detection.II. The particle-antiparticle asymmetry generation and washout.The results will have implications for most experimental probes. As the long-range dynamics becomes increasingly more important at higher scales, the investigation
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Service Web 3.0' under Seventh Framework.

assistant: **Concept**

Even after four decades of rapid advances, computing is currently subject to revolutionary changes at all levels, including hardware, middleware, network infrastructure, but more importantly intelligent applications. Emerging technologies such as the Semantic Web or Web Services transform the Internet from a network of information to a network of knowledge and services. The number of services which will be offered on the Internet is expected to rise dramatically in the next few years.<br/>It is the mission of Service Web 3.0 to address these emerging developments and contribute to the implementation of framework programmes and their projects, and support the preparation of future community research and technological development.<br/>The focus of Service Web 3.0 will be to:<br/>\tCreate, maintain, and publish roadmaps as a means to plan and coordinate framework and community activities for a future service world<br/>\tSet-up of dedicated service clusters focusing on Semantic Web services within STI2 network (Semantic Technology Institute International)<br/>\tProvide information material as white papers and feasibility studies for raising the awareness for the technology in industry, introducing new business models and systematically facilitating Semantic Web services and Semantic Web technology adoption, in particular for SMEs<br/>\tSupport standardization activities for semantic service descriptions<br/>\tCreate a European International Position Strategy for collaboration with key IT companies and coordination of strategies with regard to framework activities.<br/>\tExploit synergies through networking and cross-fertilization with other research and network projects related to this area<br/>\tOrganize special focused conferences and seminars<br/><br/>Service Web
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Stochastic Communication Inside Cortical Microcolumns' demonstrating excellence, impact and implementation quality for a Horizon 2020 call.

assistant: **Abstract**

Neurodegeneration, such as Alzheimer’s disease, currently affects 15 million people in the US with a death rate of 29.5% among 65+ years old and produces a cost around 200 billion dollars per annum. These type of pathologies are caused by neuronal communication failures in multi-scales of the cortical microcolumns. Neuroscience has historically provided theories and experiments to explain the signal propagation and neurodegeneration inside cortical microcolumns, but these behaviours are far from being fully explained. The newly formed research area of molecular communications can bring light to this challenge by using information and communication theory in this scenario. A novel interdisciplinary methodology (multi-scale modelling + neurological modelling + information theory) can analyze and quantify the dynamics in the synaptic plasticity and provide further understanding about cortical microcolumns. With this approach, STOICISM project aims to i) model the multi-scale cortical microcolumn neurology; ii) model and quantify the neuronal communication; iii) investigate the long-term plasticity dynamics and control strategies. STOICISM will develop a complete in-silico model of the 2mm cortical microcolumn that will account for its multi-scale communication. The synaptic plasticity variation will be modelled as a stochastic model based on in-vitro experimentation of the neuron-astrocyte communication.  This action requires the researcher to move to a world-center in computational biophysics, such as BioMediTech, where he can get appropriate training, perform biological experiments and collaborate with other top researchers while being supervised by the renowned leading academic Prof. Jari Hyttinen. STOICISM will change the way we understand the effects of neurodegenerat