RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Particle Nano Coater' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

In the ERC-StG-project CoCooN, we investigate the surface modification of nanoparticles by Atomic Layer Deposition (ALD). To enable this research, a rotary ALD reactor was developed. The number of possible applications for nanoparticles has strongly increased in the last decade. For many applications, such as catalysis, batteries, solid-state lighting, but also drug development and biotech, nanoparticles with different surface properties are necessary. Today, and with the help of the ERC- project CoCooN, ALD has proven to be a reliable method for depositing ultrathin and conformal coatings, even on large quantities of (nano)particles.We have designed and built a prototype rotary ALD reactor that enables both thermal and plasma-enhanced ALD surface modification of (nano)particles. In PaNaCo, we will push this rotary ALD reactor to a pre-commercial level.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Fourth Framework proposal on 'Sonar Technology for Monitoring and Assessment of Benthic Communities'.

assistant: **Concept**

The health of benthic communities is closely influenced by environmental impacts caused by human activities; at the same time many benthic communities play central roles in their respective ecosystems. Therefore, monitoring of benthic communities and the quantification of their living conditions is important, and can serve as a key indicator for sustainability of natural marine environments.  Priorities for environmental protection are strengthened these years, and the demand for information at higher resolution scales is continually rising Thus, it is vital to develop methodologies dedicated to deliver high resolution information on the health of the marine environment.  The overall objective of the sIOSONAR project is to develop and refine the fields of a. Stochastic descriptions of spatial patterns of benthic communities, b. image processing techniques, c. geostatistical estimation methods, as well as their links and interface,ending with an integrate methodology for sonar monitoring of benthic communities.  The project will include comparative studies and validation of field results used to assess the reach of the method.  Compared to existing benthic monitoring based on sampling, a successful outcome of this project would allow information to be collected at resolutions not attainable today, yet at the same cost level as that of today's monitoring based on sampling. The result will thus be a low-cost monitoring methodology delivering high resolution data for both small and large scale areas.  It is expected that data collected in the project will be valuable to other research projects dealing with use of sonar technology in environmental and/ or biological contexts. Therefore, all data will be collected in a database, to be publically accessible preferably through
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon Europe proposal on 'The human genetic and immunological determinants of the clinical manifestations of SARS-CoV-2 infection: Towards personalised medicine'.

assistant: **Concept**

The consequences of SARS-CoV-2 exposure range from a lack of infection to lethal COVID-19. This immense inter-individual clinical variability is the key scientific and medical enigma in the field. While age and certain co-morbidities are known to influence disease outcome, these parameters do not explain all variation. In addition, there are other SARS-CoV-2 phenotypes of clinical importance: multisystem inflammatory syndrome in children and adults (MIS-C/A), and longCOVID. An important breakthrough to unravel the pathogenesis of COVID-19 came from our two Science papers that were recognized by Nature among the top 10 discoveries of 2020. We found that about 4% of patients with critical COVID-19 pneumonia had inborn errors of immunity (IEI) that impair TLR3- and IRF7-dependent type I interferon (IFN) immunity and at least 10% of the patients carried pre-existing autoantibodies (auto-Abs) neutralizing type I IFNs. These findings pave the way for further studies of COVID-19 pneumonia and other SARS-CoV-2 infection phenotypes and form the basis of the present research proposal, UNDINE, which follows a ""bed side to bench"" and ""bench to bed side"" approach, with the following objectivies  i) to decipher the genetic and immunological basis of the various SARS-CoV-2 disease manifestations, to identify individuals at increased risk of critical COVID-19, post-infectious immunological complications, and vaccine failure iii) to develop ready-to-use diagnostic tests for large-scale detection of auto-Abs to type I IFNs and propose novel preventive and therapeutic approaches, based on the pathogenesis of SARS-CoV-2 infection for translation into personalised medicine. To achieve these goals, our project will coordinate a European multidisciplinary and translational research effort
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'integRated intElligent multi-modal tRanspOrt infrastrUcTurE: distributed localised decision-making at the network edge', explaining its relevance to EU policy priorities under Horizon Europe.

assistant: **Impact**

Urban areas host 70% of the EU population, and it is estimated that this will increase up to 84% by 2050, exacerbating the EU cities’ current daunting problems such as air quality and greenhouse gas (GHG) emissions. In 2019, the transport sector was accountable for 27% of total EU GHG emissions, of which road transport accounted for approximately 72%. Many cities are looking to promote public and nonmotorised forms of transport to provide access to safe, affordable, accessible, and sustainable transport systems for all citizens. Many cities in Europe and worldwide are witnessing an increasing interest in shared and multi-modal mobility services using modes such as carsharing, bikes, ride-hailing, e-scooters facilitated by the emergence of intermediary mobility-on-demand platforms. Mobility services accessible ‘on demand’ can be achieved by an integrated Multimodal Intelligent Transport System (M-ITS) incorporating both motorised and non-motorised transport as well as private and public systems through the concept of Mobility as a Service (MaaS) Sustainable mobility alternatives such as a fully integrated and seamless multimodal mobility system that can be made widely available with the communication and computation advancements. RE-ROUTE an integRated intElligent multi-modal tRanspOrt infrastrUcTurE: distributed localised decision-making at the network edge forms an international, interdisciplinary, and inter-sectoral network of institutions with complementary skills, working on a joint research and knowledge transfer programme to improve the multi-modal Mobility-as-a- Service transport integration through localised Edge-based real-time data sharing, decentralised decision making, and localised network that places the processing at the proximity of data, contribute to e
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Diagnostic autoantibody assay to stratify bone marrow failure diseases' under Horizon 2020.

assistant: **Concept**

Bone marrow failure syndromes (BMFS) such as aplastic anaemia (AA) are a heterogenous group of rare diseases that manifest as loss of red and white blood cells. In these potentially life-threatening diseases, blood producing stem cells may be inherently defective or become destroyed due to acquired cause. Diagnostic difficulties of BMFS constitute a significant clinical problem as the diagnosis takes long, and misdiagnosis may lead to inappropriate management with serious side effects. In our ERC-CoG project we made an exciting discovery and identified a novel autoantibody in AA patients’ blood samples. Autoantibody positivity defined a distinct group of patients with similar genetic background suggesting common pathogenesis. Our preliminary results show that the autoantibody analysis has great potential to reveal cases of AA and define a subset of other BMFS patients with similar underlying pathology that currently lack proper diagnosis. Furthermore, vast commercialisation potential lies in introducing it as an exclusion laboratory test in patients with unexplained anaemia or leukopenia (common problems in elderly).The improved diagnostic accuracy and the targeted personalised therapy that follows has the potential to impact the lives of hundreds of thousands of patients over time and bring simultaneous healthcare savings. Further, understanding of the role of target protein of the autoantibody in bone marrow failure may lead to targeted therapies in the future. We believe that the test can be developed as an inexpensive reagent kit to be used in serology machines for autoantibody screening. In order to improve the commercialisation potential, we will carry out 1) Technology PoC for confirming the approach validity, and 2) Pre-commercialisation actions for evaluating t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Investigating the topography of nuclear phosphoinositide signalling in relation to chromatin and the genome' (2007-2013).

assistant: **Concept**

Phosphoinositides (PIs) are an important group of phospholipid messengers that regulate diverse cellular functions. A combination of mono, bis and tris-phosphorylation generates seven different PIs present in various subcellular compartments and importantly found within the nucleus. Nuclear PI levels change in response to diverse stimuli such as differentiation, growth factor receptor activation, growth responses in vivo, cell cycle progression, DNA damage, and cellular stressors. Chromatin forms a highly ordered repressive structure that limits access to the DNA sequence.  It is widely held that disruption of chromatin compaction, which can be induced by histone tail modification is a rate-determining step in processes such as RNA transcription, DNA replication and DNA repair. Histones modification is reversible and its homeostasis is maintained by antagonistic enzymes that modify (writers) and those that remove the modification (erasers), while proteins that interact with the modifications (readers) act to interpret them.  Differential states of histone modification appear to exhibit distinct distribution patterns in mammalian chromatin and appear to define different transcriptional landscapes. We have shown that many proteins that act as writers, readers and erasers of histone marks such as PHD finger containing proteins are receptors for nuclear PIs, suggesting a role for nuclear PI in chromatin modulation. In this study I will develop and use novel technologies and proprietary specific small molecular weight inhibitors of PI-kinases, to define the topographical environment of nuclear PI-signalling in relation to different chromatin landscapes and the genome. I will then determine if and how specific modulation of nuclear PIs in different chromatin landscapes functi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Genetic and hormonal influences on reward processing: insights from brain imaging in humans' (GHIRP), addressing dissemination and exploitation.

assistant: **Impact**

Advances in molecular genetics, endocrinology and neuro-imaging start to unravel the relationships between genes, hormonal status, cognition and functional brain regions and to build new bridges between molecular, cellular and neuroscience systems levels in humans. The goals of this research program are to understand how individual genetic and hormonal variations influence reward processing in humans. Reward processing plays a fundamental role in motivation, learning and cognition. Fundamental electrophysiological results in monkeys indicate that dopaminergic neurons code both a transient reward error prediction signal, that is a discrepancy between the predicted reward and the reward effectively delivered, and a sustained signal covering with reward uncertainty that may be functionally important for risk seeking behaviour. Until recently, it was unknown whether these two modes of activities could also be observed in humans and whether they could be distinguished by post-synaptic dopaminergic projection sites.Using functional magnetic resonance imaging in a new task that systematically varied monetary reward probability, we have distinguished transient and sustained dynamics of the reward system in healthy humans. We are planning to use this same task in order to pursue the following specific aims: (1) to understand the influence of the polymorphism of the catechol-O-methyltransferase (COMT), an enzyme that metabolises released dopamine, on the pattern of brain activation during reward processing; (2) to investigate how gonadal steroid hormones affect the reward system during the normal menstrual cycle, by studying women during the follicular and luteal phases. This basic research project on the neurobiology of reward is expected to provide new insights to under stand a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Engineering protein switches: sensors and regulators for biology and diagnostics' in the context of a Seventh Framework call.

assistant: **Objectives**

Proteins that switch between distinct conformational states are ideal to monitor and control molecular processes within the complexity of living cells. Inspired by the modular design of natural signaling proteins, we are pursuing a true engineering approach towards the development of protein switches which we call ‘plug-and-play’ protein engineering. In this approach we explore generic strategies to translate molecular recognition in an input protein domain directly into a readable signal generated by an output protein domain.A comprehensive research program is proposed that explores 4 new design concepts for protein switches:1. FRET 3.0. A generic design concept for genetically encoded fluorescent sensors that does not depend on ligand-induced conformational changes in a receptor domain.2. FRET-bodies. Integration of FRET sensors and antibody technology. Libraries of FRET sensor proteins displaying CDR3-type loops on the donor and acceptor domains are displayed on yeast, allowing efficient high-throughput screening using FACS.3. ELISA in solution. An innovative and generic approach to translate antibody binding directly into an enzymatic activation step is proposed that takes advantage of the unique structural properties of antibodies4. Light-responsive protein switches. Ligand binding proteins will be developed whose affinity can be reversibly controlled by photoresponsive protein domains.An integral aspect of the research program is to combine rational design and directed evolution. Apart from developing generic engineering concepts for specific application areas, modeling tools will be developed that allow quantitative analysis and prediction of conformational stabilities for modular protein switches. The availability of these robust and generally applicable enginee
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Novel approaches for the development of new and sensitive molecular diagnosis for detection of sulfate reducing bacteria in environmental samples like oil' (1994-1998) targeting Fourth Framework.

assistant: **Abstract**

SRB would be identified by genetic fingerprint and detected by molecular hybridization and/or polymerase chain reaction (PCR). Furthermore, incubation time before analysis could be canceled or signifcantly reduced. rRNAs and related genes play an important role in micro-organisms identification because they combine many advantages. Analysis of rRNAs sequences is one of the main concept in bacterial populations studies. rRNAs are abundant in each bacteria (e.g.: 10,000 copies in E. coli). rRNAs sequences present regions with high variability useful for determination of specific probes for each microbial species. They also present more conserved region leading to determination of specific probes for groups or bacterial species. rRNAs are easily used as targets for PCR amplification, which could allow detection of viable but non cultivable species. Furthermore PCR amplifications of variables regions lead to a synthesis of new probes. Many studies have been realized in many laboratories (more than 4,000 sequences of 16S rRNAs specific of each bacterial species). Each bacterial cell contains important quantities of rRNAs which could be detected by in situ hybridization. We will try to develop fluorescent probes species-specific or taxon-specific for enumeration of diffent SRB. Membranes treatment will be developed and associated with computing associated images analysis systems. PCR is a very powerful tool to obtain and to analyze high quantities of target DNA sequences. Its high sensitivity is important when there are few bateria and/or when biological samples are not cultivable. Furthermore, its high quickness is particularly interesting in the case of emergency diagnosis and when a short delay is allowed (traditional microbiological analysis require a long time before res
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'CLINICAL REHABILITATION USING ELECTRICAL STIMULATION VIA TELEMATICS' to be submitted under Fourth Framework.

assistant: **Abstract**

Functional electrical stimulation (FES) can help incomplete Spinal Cord Injured (SCI) users to walk better, but lack of knowledge about how to get the best use from equipment hampers its deployment in daily rehabilitation care. CREST will overcome this by facilitating communication between user groups throughout Europe, demonstrating that interactive tele consultation with an expert can enable FES systems to be delivered by non-expert professional users. Standardised multimedia assessment, teleconsultation, individualized walking strategies, and visual programming environments are its key developments, enabling a good uptake of pan-European individualised prescription of FES systems for incomplete SCI patients. It is envisaged that the system could also apply to other large categories of conditions like CP, stroke, and MS.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Study the therapeutic and preventive potential of targeting oncogenic mutations with CRISPR-Cas9 technology' goes beyond current state-of-the-art under Horizon 2020.

assistant: **Innovation**

Genome editing has enriched our understanding of mechanisms of the human pathology. Genome editing took a significant advance with the recent development of the CRISPR-Cas9 technology. CRISPR is an acronym for: Clustered Regularly Interspaced Short Palindromic Repeats and it is an adaptation of a prokaryotic functional system. It uses a single guide RNA to direct Cas9 activity to a specific part of the genome, therefore, this system can be used for gene editing and regulation.Cancer is a genetic disease where some DNA-damaged cells begin to divide without stopping and spread into surrounding tissues. Interestingly, in some tumors, there is a dependency of a single oncogenic activity (oncogene addiction). This phenomenon indicates that mutations in key oncogenes (driver mutation) are able to drive carcinogenesis and maintain the tumor phenotype. Suggestively, if we can prevent or disrupt these mutations, we can difficult carcinogenesis or damage an established tumoral phenotype. In this proposal, we seek out for using Crispr-Cas9 technology to target driver mutations and evaluate its therapeutic and preventive value. To develop a proof of concept, we will focus on lung cancer driven by KRAS mutations. The generating of a transgenic mouse expressing Crispr-Cas9 designed to target the mutation Kras C12C will allow us to test the potential cancer-resistant phenotype and raise the concept of Genetic Vaccines.