RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Studying hybrid speciation and introgression using a natural fungal model system' (FINESSE).

assistant: **Concept**

Hybridisation, mating between distinct species or divergent populations, can lead to a variety of different outcomes. Many hybrid offspring will have low fitness or be inviable, while others may increase fitness under extreme conditions by producing transgressive hybrids with phenotypes outside of their parental range. The role of hybridisation in adaptation and speciation is a key question in evolutionary biology, especially as hybridisation is predicted to rise due to climate change and other human activities.In FINESSE (Fungal INtrogrESsion and SpEciation) we aim to use the wood-decaying fungus Trichaptum abietinum as a study system for researching hybridisation. In Europe, there are three distinct populations of T. abietinum: a Mediterranean population, a Boreal, and an Atlantic population. Where populations overlap, there are different hybrid outcomes with hybrids found in central Europe but not Norway. These contrasting outcomes make our system ideal to study patterns of introgression and hybridisation in Europe.During FINESSE we plan to 1) gather and sequence field samples from underrepresented regions across Europe, both to explore the diversity of these populations in Eastern Europe and identify the origin of the Atlantic population in Norway. 2) Generate hybrids in the lab to help us understand the differing hybrid outcomes found in Europe, and identify mechanisms of hybrid incompatibility through genomic and phenotypic assays. 3) Use a reverse genetics approach to identify genes associated with hybrid incompatibilities, using CRISPR-Cas9 transformation of candidate genes. I will also use the project to improve both my scientific and transferable skills by taking courses, from other researchers in the department, during management of the project, and more gene
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'New players in human BAT differentiation and activation: a human PSC-derived BAT approach combined with state of the art genome engineering and –omics based methodologies' (StemBAT), addressing dissemination and exploitation.

assistant: **Impact**

Here we propose a strategy to control body weight and prevent/reverse obesity based on targeting brown adipose tissue (BAT) to facilitate negative energy balance and prevent adaptive responses to dietary restriction. However, BAT in humans is limited and poorly characterised. Thus, we propose to use stem cells as a tool to gain unique insights into the biology of human brown adipocytes. The General Objective is to identify pathways and factors of potential therapeutic relevance that promote BAT development and/or activation/recruitment using a stem cell based BAT differentiation approach, and then to functionally validate the role of these factors in vitro by genome engineering human stem cell derived adipocytes and in vivo by transplanting these cells into mice. Specific Aims are: 1.To identify molecular mechanisms involved in human brown adipose tissue development and activation. 2. To investigate the molecular mechanisms involved in human white adipose tissue browning/beige cells recruitment 3. To identify new agents/compounds of therapeutic value, able to activate or recruit human brown adipose tissue/brite cells.Experimental strategy: We will use a UCP1-reporter human pluripotent stem cell (PSC) line differentiated into brown and white adipocytes to identify genetic factors that may contribute to brown adipocyte differentiation/activation and white adipocyte browning. Following the identification of candidate genes, we will knock out, constitutively and/or inducibly, both alleles of these genes in human PSC cells, producing a total loss of function. Following the in vitro phenotyping of the cells we will proceed to the in vivo validation of the functional properties/phenotype of human PSC derived brown/brite adipocytes (wild-type and loss of function) by transplant
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Portable, high-performance, and all-in-one digital data recovery lab for digital forensics to greatly enhance efficiency and capabilities of European investigation authorities' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

The digital revolution is fundamentally changing the world regarding quality of life, efficiency of our economy, communication and data handling. It’s not surprising, that digital data is nowadays - intentionally or not - involved in most criminal activities and is of upmost importance in the investigation process. Moreover, with cyber-crimes, a whole new area of criminal offences is emerging.To face this fast-growing threat, and close the “digital gap” in investigations, law enforcement agencies established digital forensics units to find, extract and analyse digital data for fighting crimes, terrorism, espionage and improve cyber defence. Here, forensics experts are also confronted with damaged disks, deleted or compromised data. Most of them are not even looked upon, because forensic units are understaffed, lack data recovery specialists and don’t have the necessary hard- and software infrastructure, to legally suitable extract the evidence inhouse. This could mean the failure of the investigation or a crucial time delay!mh SERVICE, a leading specialist for digital forensics, developed the fastest, most efficient and powerful data recovery device that ever existed: SALUS. The portable system is designed for usage at any forensics lab and in the field by trained forensics experts. The system covers nearly all types of media interfaces, data storage technologies and recovery techniques. The fool-proof software guidance and performant hardware allows to significantly enhance efficiency and capabilities of forensic units.In the feasibility study, a detailed analysis of the best-fitting market segments within the IT forensics market will be conducted, including the involvement of pilot customers for the validation of the business idea, as well as the elaboration of a thor
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Fourth Framework proposal on 'Role of the angiopoietin system in tumor angiogenesis'.

assistant: **Concept**

Rescarch objectives and content Tumors are usually not angiogenic at the beginning of their development. and conseauentely they remain limited to small volumes. The acquisition of the angiogenic activity depends on the balance of positive and negative angiogenic factors released by the tumors. The aim of this project is the identification and characterization of the molecular events that are involved in the onset of tumor angiognenesis. All the studies will be performed using as a model a transgenic mouse line expresses SV-1O large T-antigen (Tag) under the control of the rat insulin promoter (Rip1 Tag2). These mice develop tumors in the Lanoherans acells in a predictable manner and different stage of tumor progression are clearly distinguishable, thus, make them a valuable tool to study the molecular mechanism of tumor angiogenesis.Expression and functional studies of the TIE-2 receptor and its ligands angiopoietin-1 and angiopoietin-2 during tumor progression in Rip1Tag2 transgenic mice will be performed. The long term goal of the project is to determine the role of angiopoietins in tumor angiogenesis in vivo. In order to reach the aim we will generate transgenic mouse lines that overexpress angiopoietin-1 or anoiopoietin-2 specifically in the ,B cells under the control of the insulin promoter. In case the forced expression of angiopoietin has no impact on the development of islets of Langherans. these mouse lines will be crossed to Rip tag2 transgenic mice and potential effects on a cell tumorigenesis will be analyzed ..Training content (objective,benefit and expected impact) The aim of this project is the identification and characterization of the molecular events that are involved in the onset of tumor angiogenesis. In particular. I propose to investigate the funct
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Thematic network for ultraviolet measurements', explaining its relevance to EU policy priorities under Fourth Framework.

assistant: **Impact**

Ultraviolet (UV) radiation is highly energetic electromagnetic radiation in the wavelength region of 100-400 nm. Uv radiation changes properties of many materials and has therefore recently found several applications in e.g. medical,cosmetic, testing and manufacturing industry. On the other hand, UV radiation also affects organic tissues. Therefore, high UV levels from e.g. sun or welding equipment cause health risks. Concerns of the environmental and health effects of solar UV radiation penetrating into the biosphere through the depleted ozono layer have greatly emphasised the urge for accurate and reliable UV radiometry.In recent years, radiometry at visible wavelengths has experienced significant progress. Improvement of primary and transfer standards has enabled morereliable measurements throughout the traceability chain. However, the progressof measurements at UV wavelengths has been slower. Part of the problems are associated with the standards since both sources and detectors tend to be unstable in the UV. Especially, there is lack of reliable easy-to-use calibration methods for needs of industry.The thematic network for UV measurements is aimed to enhance exchange of results and ideas between the research laboratories and industry and also to obtain a better understanding of the problems associated with the calibration and testing work.The network has participants from all branches of UV measurements. All majorEuropean national standards laboratories, equipment manufactures and end-users of equipment and services are involved. The thematic network organises four workshops devoted to outlining and seeking solutions to problems in UV measurements. Four working groups will be established to work on specific subjects that are found most essential. Training courses w
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Green Engineering, Analysis, & Reskilling for Unbounded Production' (GEAR-UP).

assistant: **Innovation**

The GEAR-UP proposal, is a comprehensive initiative to revolutionize the manufacturing sector through sustainable practices and advanced technologies. The project's central objective is to develop digital tools and methodologies that address recycled materials variability. It covers stainless steel, aluminum alloys, and fiber-reinforced plastics for additive manufacturing (AM). It employs simulation-driven approaches for optimizing various AM processes, including laser beam-directed energy deposition, metal laser beam powder bed fusion, and fiber-reinforced polymer material extrusion.GEAR-UP also emphasizes environmental conservation by using recycled materials in engineering design, significantly reducing virgin resources, energy consumption, and greenhouse gas emissions. The project intends to overcome challenges associated with secondary materials, such as performance variability, through resilient design and life-cycle environmental impact assessment.Moreover, the proposal outlines ambitious objectives for enhancing sustainable product design using innovative simulation and modeling software. It also implements the Digital Product Passport initiative and fosters human involvement in advancing circularity and sustainable technology adoption. This is done through training and global collaborative networks. GEAR-UP's approach represents a paradigm shift in engineering design, focusing on the circular value chain and developing universally adaptable AM technologies resilient to material variability. This endeavor is expected to directly impact the high-performance consumer products, green energy, & high-tech robotics markets. The key outcomes are:Cost savings: 25 %Increased productivity: 20-50 %Extended fatigue life: 50-80 %Defects reduced: 50-80 %Material waste reduced
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Fifth Framework proposal on 'Mix mode solid-state inline analogue line interface for asymmetric DIGItal Subscriber LInes.'.

assistant: **Concept**

Existing telephone infrastructure worldwide is being developed to offer communications capabilities to a wider audience and improve communication services to open up many new applications. ADSL is emerging as a key technological advancement increasing transmission bandwidths enabling new high-speed applications, improving quality of life and opening doors to electronic commerce. ADSL services today have limitations due to reliance on 50 years old technology to provide the required functions of safety isolation, impedance matching and signal filtering. This limits the distance over which ADSL can be transmitted and therefore restricts the availability of the service. Additionally, these old technologies are labour intensive and unreliable by the standards needed for the next 25 years. This project will overcome these inherited problems through the development of specialist new materials and manufacturing processes.Objectives:Current line interfaces implemented in ADSL equipment are based on 50 year old technology utilising a ferrite based line isolation transformer to provide an electrical safety barrier. The transformers typically are very large in volume and very heavy relative to the rest of the circuit and are very labour intensive in their manufacture. Specifically they have a volume of approximately 1.30sqcm, weigh 0.003kg and each take approximately 5 minutes to produce. As would be expected, a device which is virtually hand made also exhibits a fairly wide spread of key parameters such as inductance which is typically specified no better than +/-5% from nominal value.In addition to the transformer, line interface circuits also include discrete inductor and capacitor components which combine to act as filters which attempt to improve the noise to signal ratio of t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Projections of Jerusalem in Europe: A Monumental Network' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

This project concerns the monumental, multimedia, interactive re-creations of Jerusalem in Europe. These monuments represent the loca sancta through architectures in spatial and topographical relationships that reproduce the real ones, sculptural groups that re-enact the respective event in a painted décor, with artifacts completing the ambience. The project proposes to document, comprehensively study and conceptualize these sites. Their exact number is not known since a comprehensive study has never yet been attempted, but they can probably be numbered in many hundreds. This project will open up a totally new research area with considerable impact already at the initial documentation and survey stages, by bringing together both fresh data and interpretations. But the project proposes to go beyond and generate many new insights by a comparative study of the sites and a conceptualization of the phenomenon (with the aid of notions such as icon, map, network, interactive multimedia, space and place). The study will lead to the integration of these three-dimensional complexes in the ongoing discourse of image, and to the theoretical discourse pertaining to the relationships between original and copy, narrative and iconic. It will contribute essential new insights to the study of pilgrimage, the definition and process of the formation of sacred spaces, to devotionalism as a special type of piety and to the study of memory and mnemonic practices. Research will include field and textual documentation, cross referencing and grouping of related monuments, leading to crystallization of several clusters of monuments, representative at Pan-European and historical levels. Research will be accompanied by a conclusion-reaching process through discussions in small groups, exchange of w
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Soil bioremediation through flagellated bacteria: unravelling the mechanisms for enhancing bacterial tactic response' (BIOTAC).

assistant: **Innovation**

Bacterial dispersal is a key driver of pollutant turnover in contaminated soils. Flagellated bacteria able to degrade organic pollutants hold tactic responses to a variety of stimuli and reach pollutant hotspots, enhancing the access to poorly bioavailable carbon sources by steepening pollutant concentration gradients at interfaces. However, bacterial motility in porous media is often restricted due to high cell deposition rates and adhesion to soil particles, and in this sense, the modulating role of biological and chemical effectors is decisive. The aim of BIOTAC is the study of the mechanisms operating with flagellated bacteria for bioavailability enhancements and, therefore, a faster turnover of persistent organic pollutants in soils, including polycyclic aromatic hydrocarbons and a pesticide, lindane. We will study, at the microorganism scale, the role of bacterial taxis in chemical gradients (chemotaxis), as well as other tactic responses (repellence, surface taxis) in pollutant bioavailability, modelling and integrating them with other microbial adaptations (i.e. biofilm formation and the production of biosurfactants). We will develop strategies to improve bacterial transport to pollutant sources, using carefully selected biological and chemical effectors (respectively including, mycelial networks and organic compounds -root exudates, humic acids, and fertilizers), that favour flagella-mediated taxis, and co-mobilization of immotile degrading strains. The originality and innovation of the project lies on the macroscale projection of the microscale mechanisms underlying the access of self-propelled bacteria to limitedly available contaminants through an enhanced dispersal. Through experimental and modelling approaches, aiming at balancing biosorption and colloidal
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Solving The Pathway of LADDERane biosynthesis' under Horizon 2020.

assistant: **Concept**

Ladderanes are highly unusual hydrocarbon moieties with linearly concatenated cyclobutane rings. They are found in the membranes of bacteria performing anaerobic ammonium oxidation (“anammox”). How these complicated molecules are produced is expected to involve both novel enzymes and specially evolved versions of known proteins, but the details remain enigmatic. How do these enzymes assemble such intricate carbon skeletons?  How do they control the complex stereochemistry involved? How do they deal with the typical nonreactivity of hydrocarbons? And how do they overcome the ring strain inherent in these molecules? Answering these and other questions about the molecular mechanism of ladderane biosynthesis will open up new frontiers in enzymology. I therefore propose to elucidate the pathway of ladderane biosynthesis. I will use a synergistic approach, combining biochemistry, chemical biology and structural biology to arrive at a comprehensive view of ladderane biosynthesis in molecular detail. Heterologous expression of selected biosynthetic gene clusters will be used to study ladderane biosynthesis in vivo. Chemical biology will be used to load ladderanes and their intermediates onto carrier proteins to study their interactions with enzymes from the biosynthetic pathway in vitro. Protein crystallography will elucidate structures of enzymes and their complexes with ladderane-loaded carrier proteins. The knowledge accumulated will not only break new ground in enzymology, but also serve as a stepping stone for the use of these enzymes in bioinspired organic synthesis.


**Keywords:** chemical biology