RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon 2020 proposal on 'GENome Editing and delivery Strategies for REcoding the mammalian genome'.

assistant: **Innovation**

The assembly of functional synthetic mammalian genomes is the most ambitious biotechnological challenge of the current century. Synthetic genomics has generated remarkable results in viruses and bacteria, but the de novo assembly of eukaryotic genomes is still limited to a few small chromosomes. Indeed, efficient delivery and genome engineering strategies for systematic genome replacement and assembly in higher eukaryotic cells are still missing. With my project RE-GENESis, I intend to make an essential contribution toward a more comprehensive understanding on the mammalian genome through the synthetic replacement of recoded loci at megabase-scale. My main goal is to demonstrate the feasibility of synthetic genomics and genetic code reprogramming at the megabase-scale in mammalian cells, using mouse embryonic stem cells to identify efficient delivery vectors, genome editing strategies and recoding schemes. I will use CRISPR-Cas in combination with large vectors to find new genome surgery strategies for genome recoding and gene correction and exploit them to replace the 2.4 Mb DMD (Duchenne Muscular Dystrophy)/dystrophin gene as a landmark application. The developed delivery and DNA replacement strategies will be also readily available for genome editing therapeutic approaches in order to correct most of the DMD disease-related mutations in human stem cells. RE-GENESis will thus pave the way for the effective deployment of synthetic genomics in high eukaryotes, expanding our understanding of complex eukaryotic genomes, delivering new tools to modify and regulate mammalian DNA and providing innovative solutions to treat genetic diseases, epidemics and ageing. This highly original and multidisciplinary project combines the strength of CRISPR-Cas with the power of large del
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Integrated bioprocess design for large scale production and isolation of recombinant proteins' will create measurable scientific, societal and economic impact aligned with Fourth Framework.

assistant: **Impact**

For selective purification of proteins, the only separation technique used broadly in industry today is chromatography, which is characterized by low capacity and poor scaleability. It must also be preceded by unit operations like centrifugation, filtration and precipitation, leading to overall low process yields. There is thus a strong need for alternative methods which are predictable, easily scaleable, and also selective, to produce pure proteins. Liquid-liquid extraction in aqueous two-phase systems presents these possibilities and has already been proven useful for some industrial separations. However, there is a need for further development of this technology for purification of recombinant proteins: selectivity in protein extraction needs to be increased, phase recycling needs to be improved for reduction of costs. The proposed research concerns the development of generic technology for integrated bioprocesses for protein production and isolation. This will be done by considering the downstream processing of the target protein already at the genetic level. Genetic engineering will be used for fusion of peptide or protein tags to the target protein, taking care to retain the protein biological activity. The modifications will be exploited for designing selective extraction systems for the target proteins. Two industrially important target proteins, a cellulase (endoglucanase) and a lipase (cutinase) will be studied. The project combines efforts of leading European research groups within the different areas described below, and involves the active participation of two biotechnological industries. The work is planned for multidisciplinary interaction, from genetic engineering and protein properties to phase partitioning and biochemical engineering, in order to desig
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Area-selective Deposition-enabled ultiMAte extensIon of lithogRaphy' (ADMAIR).

assistant: **Innovation**

Nanopatterning is the core of today’s and future semiconductor technology as it drives the downscaling of the devices below the 7 nm node, enabling higher speed, density of transistors and computing performance. In addition, nanofabricated three-dimensional patterned structures are used in devices for photonics, biotechnology and other forms of nanotechnology.[1]Beyond the 10nm node, extreme ultraviolet lithography (EUVL) with a light source of 13.5 nm, will be applied.[2],[3] Associated with nano-patterning at these dimensions, there is a long list of challenges: i) EUVL photoresists (PR) and mask infrastructure and control of the created nanoscale patterns; ii) throughput and process cost; iii) environmental footprint as it is based on litho-etch subtractive processes; iv) high complexity thus decreasing fabrication reliability; v) overlay errors and defectivity. Therefore, innovative solutions are required.  Area selective deposition (ASD) offers the potential to relax downstream processing steps by enabling self-aligned processes and bottom-up lithography. The main goal of this MSCA proposal Area-selective Deposition-enabled ultiMAte extensIon of lithogRaphy (ADMAIR) is to overcome the potentially show-stopping challenges associated with EUV materials and processes at the sub-10nm scale by an additive bottom-up concept. This will be enabled by interdisciplinary joint development projects involving material suppliers and lithography stakeholders, which is a unique advantage of IMEC and will complement the expertise of the candidate in material science and characterization. Ultimately, valuable professional development opportunities for the MSCA candidate and know-how for the host will be achieved.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Smart Automation of Rail Transport' (SMART).

assistant: **Innovation**

SMART main goal is to increase the quality of rail freight, as well as its effectiveness and capacity, through the contribution to automation of railway cargo haul at European railways.In order to achieve the main goal, SMART will deliver the following measurable objectives:• complete, safe and reliable prototype solution for obstacle detection and initiation of long distance forward-looking braking, • short distance wagon recognition for shunting onto buffers,• development of a real-time marshalling yard management system integrated into IT platform available at the market.The SMART prototype solution for obstacle detection will provide prototype hardware and software algorithms for obstacle detection. The system will combine two night vision technologies - thermal camera and image intensifier with multi-stereo vision system and laser scanner in order to create fusion system for mid (up to 200 m) and long range ( up to 1000 m) obstacle detection during day and night operation, as well as operation during impaired visibility. By this planned fusion of sensors, the system will be capable, beside reliable detection of obstacle up to 1000 m, to provide short range (< 200 m) wagon recognition for shunting operations with a +/- 5 cm distance estimation tolerance. The real-time marshalling yard management system will provide optimization of available resources and planning of marshalling operations in order to decrease overall transport time and costs associated with cargo handling. The yard management system will provide real time data about resources available over open and TAF/TSI standard  data formats for connection to external network systems and shared usage of marshalling yards between different service providers.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Targeted Experiment to Reconcile Increased Freshwater with Increased Convection' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

The ocean Meridional Overturning Circulation (MOC) is  responsible for poleward heat transport, and deep storage of heat and carbon.  Climate models generally predict that a slowdown of the MOC will occur this century, with dramatic regional and global climate changes,  but how the slowdown will occur is subject to debate.  It is widely recognised that convection -- the downward limb of the MOC -- is sensitive to freshwater fluxes, and recent investigations have suggested that the increased melt from the Greenland Ice Sheet has already reduced convection.  Yet in 2015, convection returned, and was anomalously strong.  Despite the expectation that the MOC is sensitive to freshwater forcing, we do not understand the processes by which  freshwater inputs influence the MOC.The two key gaps in our understanding are: how freshwater reaches the regions of deep convection, and the relative importance of freshwater to convection and restratification. Numerical simulations give conflicting results on  freshwater pathways, and convective parameterisations neglect small-scale restratifying processes. Traditional observational approaches cannot  capture the spatial and temporal variability of these processes  without inordinate cost.TERIFIC addresses these gaps in understanding through new observations, leveraging recent advances in small-scale electronics to  deploy large numbers of mini-drifters on the shelves of Greenland, and employing subsurface and surface autonomous platforms to characterise the balance of processes controlling convection and restratification. The analysis of these observations will answer fundamental questions about how freshwater reaches and affects the regions of deep convection, and enable a critical ground truth of numerical simulations of these processe
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Role of ret and modifier genes in human disease' will create measurable scientific, societal and economic impact aligned with Fourth Framework.

assistant: **Impact**

- To map modifiers of Hirschsprung disease (HSCR) and papillary thyroid carcinomas (PTC) in mice and in humans. - To construct new ret knock-outs. - To further develop a mouse neural crest culture system. - To identify the biological and biochemical effects of HSCR RET mutations in an in vitro expression system which will be integrated by the use of a panel of recombinant molecules known to be involved in receptor tyrosine kinase (RTK) triggered pathways. - To identify possible interactions between the RET pathway and that mediated by the endothelin receptor B (EDNRB). - To establish the developmental potentials of enteoic nervous system (ENS) progenitors and rescue of the aganglionic gut phenotype of ret knock-out homozygous embryos by introducing into the embryo ret+ positive progenitors.The RET proto-oncogene is at the origin of one of the most interesting models of human disease caused by mutations in a receptor tyrosine kinase gene. RET mutations or heterozygous deletions of the whole gene cause the autosomal dominant form of congenital megacolon or Hirschsprung disease (HSCR), a relatively frequent genetic malformation of the enteric nervous system. Somaticre arrangements of RET are involved in the aetiology of a variable proportion of papillary thyroid carcinomas (PTC), the most common type of thyroid tumour whose prevalence is increasing in areas heavily exposed to radioactive fallout after the Chernobyl accident in 1986. Moreover, germline RET mutations are associated with the three variants of the rare inherited cancer syndrome Multiple Endocrine Neoplasia type 2 (MEN2A, MEN2B) and Familial Medullary Thyroid Cancer or FMTC. These different effects of RET mutations have been correlated with loss of function or gain of function mechanisms using in vitro expressi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Decoding Death and Dying in people with Dementia by Digital thanotyping' (5-D).

assistant: **Innovation**

How can we recognize that a person with dementia is at the end of life? When we are dying, our physical, mental, and social abilities are gradually declining. No reliable method of predicting perceived dying currently exists although the technology is available (sensors, algorithms). The aim of Decoding Death and Dying in Dementia by Digital thanotyping (5-D) is to provide methods and tools to diagnose and describe dying to an unprecedented level of accuracy and robustness, within a timespan larger than is possible now, focusing on the case of dying people with dementia as one of the most vulnerable and difficult to study groups. 5-D combines clinical assessment tools with wearable sensing technology to monitor a) pain and distressing symptoms, b) behavioral and psychological symptoms in dementia (BPSD), c) oral changes, and to decode “the point of no return” as the beginning of perceived dying. To obtain this outcome in nursing home patients with dementia, I will test the main hypothesis: from monitoring the evolution of thanotype components over time and their interdependencies, the prediction of the “point of no return” is possible. The objectives of 5-D are:O1. Collect data using sensors and validated assessment scales.  O2. Develop estimation methods for BPSD from sensor measurements. O3. Develop digital tools to capture the expression of pain.O4. Determine the relationship between breathing and oral symptoms. O5. Develop models for symptom interdependencies at the end of life and the “point of no return”.  O6. Perform human-in-the-loop validation of developed tools, models, and algorithms.The ground-breaking interdisciplinary novelty of 5-D endeavors to enhance our understanding of end-of-life underlying pain and symptoms in people with dementia. Advancing our the
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon Europe proposal on 'Experimental Continuation in Nonlinear Dynamics: Aerospace Engineering and Beyond'.

assistant: **Innovation**

Nonlinear vibration theory witnessed extraordinary advances during the 20th century following Poincaré’s seminal work. Since the 1970s, impressive progress has been made in computational nonlinear dynamics with the development of nonlinear finite element methods and numerical continuation. Although not all challenges have been overcome yet, the theoretical understanding of nonlinear dynamical phenomena and their prediction using numerical models have reached a high level of maturity. Surprisingly, this progress has not significantly impacted engineering practice. Specifically, vibration testing in industry remains grounded in the assumption of linearity. Yet, under the rising pressure of high-performance and carbon-neutral designs, aerospace structures exploit advanced materials and are increasingly lighter and more flexible, with the consequence that nonlinearity is now ubiquitous.  To fill the existing gap, a vibration testing strategy which can uncover the sometimes dangerous, sometimes beneficial nonlinear dynamics of engineering structures is urgently needed. The ENTIRE project proposes to leverage feedback control to pioneer a novel methodology able to identify experimentally - in a model-free, real-time and stability-guaranteed manner - the entire bifurcation diagram of a nonlinear system. This control-based method will be complemented by a data-driven, machine-learning-based approach. The potential of our developments will be demonstrated using real-life aerospace applications including turbojet engine components and a full-scale aircraft. Given the generic character of our methodology, applications in other areas of science and engineering will also be envisioned, e.g., in microresonators and in dress of physics such as optics. Eventually, ENTIRE will develop t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Network-based prediction of spreading of tau pathology in Alzheimer’s disease: implications for clinical trials' (2021-2027) under Horizon Europe.

assistant: **Objectives**

Alzheimer’s disease (AD) has far-reaching societal and economic consequences, emphasizing the urgent need for effective treatment solutions. However, to date, over 99% of AD clinical trials have failed. Inadequate patient stratification is a key contributing factor due to the enormous clinical and pathological heterogeneity in AD. AD is characterized by the aggregation of pathogenic proteins, with tau aggregates being a key player. Tau-related pathological processes lead to synaptic dysfunction, neurodegeneration, and eventually dementia. It is thus of paramount importance to decipher the mechanisms of tau deposition and spread. Emerging evidence suggests that tau tangles accumulate along specific macroscale brain networks, lending support to the hypothesis of tau spreading through communicating neurons. Network-based spreading models have provided insights along this line. I aim to contribute to a leap in network-based modelling of tau spread, by developing a novel patient-specific network model, coupled with a diffusion framework to study tau spread. The model will be individualised, in contrast to previously used group-averaged designs, and will be hybrid, in two respects. First, the model will integrate global functional and structural connectivity. Second, it will integrate data on regional vulnerability of brain tissue for generating tau based on gene expression profiles from an atlas. Results will provide mechanistic insights into spread of tau. The model and its subtypes will then be used to define an optimal sample stratification scheme, to enhance patient selection in clinical trial designs. Importantly, designs and analysis methods developed as part of this action will be released as a unified open-source software package to allow replication, expansion, and
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Development of an innovative ground penetrating radar for recognition and identification of subsurface buried objects' (SMART-RAD) targeting Fifth Framework.

assistant: **Abstract**

Ground Penetrating Radar (GPR) has proven to be the best candidate for non- invasive sub-surface analysis. In response to specific limitations of existing systems, the SMART-RAD project intends to develop an innovative technology able to set forth the conditions for a widespread commercialisation of GPR tools within a broad range of industrial sectors where efficient underground inspection is required. SMART-RAD will be able to reliably detect buried objects and will have easy on-field data interpretation capability, such to be used by untrained operators. The system will have a broad class of potential users in the construction and re-development industry, as efficient and cheap alternative to conventional ground inspection methods in construction or de-commissioning sites, reducing the risk of damages and accidents during excavation operations.