RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Reduction of microalgae harvesting costs via the development of an ultrasound flow cell to provide pre-concentration' goes beyond current state-of-the-art under Seventh Framework.

assistant: **Innovation**

The worldwide production of microalgae is currently around 5,000 tons per year of dry algal biomass, and is used for livestock feed, fish meal, and high value extracts for dietary supplements, functional foods, cosmetics and pharmaceuticals. Microalgae contain around 30% by weight of lipids/oil, and are regarded as a highly promising feedstock for biofuels, primarily biodiesel.Microalgae are commercially grown in open ponds or bioreactors, and then harvested using a centrifuge separator. The centrifuge step is highly energy intensive, due to the properties of the microalgae and their low concentration in the growing medium (water), and represents a major obstacle to the economic viability of microalgae biodiesel production. The capital investment required for the centrifuge is also a barrier for widespread SME entry to the sector.The ALGAEMAX project aims to address these issues via the development and demonstration of an innovative and cost effective ultrasound flow cell to concentrate the microalgae, with the aim of eliminating the centrifuge component.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Sub-sea operations with autonomous vehicles' (SOAVE).

assistant: **Innovation**

The primary objective is to guarantee economic and efficient energy for a competitive Europe in the short term by addressing the difficulties of developing European hydrocarbon resources, related to the technical limitations and excessive cost of performing sub-sea operations in deep waters (up to 3000 metres). Currently these activities are performed by Remotely Operated Vehicles (ROVs), which are connected to a surface support vessel by umbilicals.The project shall develop a system to permit these activities to be carried out with Autonomous Underwater Vehicles (AUVs), which do not have any physical connection to the surface and thus do not require dedicated surface support, permitting the operations from the existing infrastructure in the work area, typically a floating production system, leading to cost saving, continuous monitoring and faster reaction to accidents. The system aims at a depth rating of 3000m, a 20km step out range, 5 hours free swimming and global IMR capability.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Fluorescent biosensors of organelle morphology to study the nuclear envelope dynamics during cell division', explaining its relevance to EU policy priorities under Seventh Framework.

assistant: **Impact**

Eukaryotic cells are compartmentalized into membrane-bound organelles, ensuring highly specialized and essential functions such as protection of the genome, protein synthesis and packaging, intracellular calcium signalling... Most organelles harbour a complex morphology, which is an important structural feature for the achievement of their assigned function. Moreover, some organelles are constituted of distinct subcompartments exhibiting specific structures associated to specialized functions. Visualizing the fine structure of organelles is thus essential to understand many aspects of their associated functions. Importantly, organelles architecture is highly dynamic and can be subjected to dramatic rearrangements in response to cellular changes like cell division or differentiation. The methods available so far to render organelle morphology require highly specialized techniques and are most often incompatible with high-rate image acquisition. The aim of this proposal is to develop a method to distinguish organelle substructures in live cells using a simple bi-dimensional confocal imaging setup. Fluorescent probes of organelle morphology will be designed based on the striking membrane sensing properties of a family of amphitpathic helices called ALPS motifs. To establish the usefulness of these tools, we propose to study the dynamics of the nuclear envelope (NE) and endoplasmic reticulum (ER) during mitosis.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Classification of retreaded truck tyres in order to comply with future environmental performance and safety requirements' (RETYRE).

assistant: **Innovation**

In this project is considered the possible impact of new European regulations–regarding new tyres safety and environmental performances–on the SMEs working with retreaded tyres. One expected consequence of these on new tyres will be the slow decreasing of the interest on retreaded tyres in the market. This will compromise the future activity of SMEs working in the field, and also determine a fatal risk for the smallest enterprises.Therefore, it is of vital importance for the retreaded truck tyre sector (400 companies, of which 95% SMEs and 14 SME associations/groupings in Europe who allow 50% of European trucks to drive on retreaded tyres) to find a scientifically sound and cost effective methodology for classification of retreaded truck tyres in order to comply with future environmental performance and safety requirements.To maintain the standard in a long-term prospective, and hopefully even increase the market for retreaded tyres, it is necessary to provide clear statistic and reproducible results on the retreads performances. Only in such a way it would be possible to compete with the new tyre market.The concept is to investigate all relevant parameters related to casings, retreading process and treads applied and to determine the influence thereof on the tyre performance. And to find a scientifically sound and cost effective methodology (important aspect of the methodology is that it is self supporting/developing) for classification of retreaded truck tyres in order to comply with future environmental performance and safety requirements.The overall objective is to find a scientifically sound and cost effective methodology for classification of retreaded truck tyres in order to comply with future environmental performance and safety requirements.The outcome of the t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Deciphering the origins of cell differentiation and developmental gene regulation in animals' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

During animal development the process of cell differentiation gives rise to terminally differentiated cell types with cell-type-specific gene expression programs. In the most closely related group to animals, the choanoflagellates, cell differentiation occurs but cells do not commit irreversibly to specific fates. Instead, they transition through cell identities as they progress through their life cycle, known as temporal cell differentiation. Animal cell differentiation is thought to have evolved from temporal differentiation, but the mechanisms are largely unknown due to a lack of information on the molecular processes underpinning temporal differentiation.This proposal will test the hypothesis that chromatin-based transcriptional regulation underlies temporal cell differentiation in choanoflagellates. Utilizing S. rosetta as a model, I will compare transcription and chromatin across cell types using genome-wide omics techniques. This will allow me to assess the role of different histone modifications in cell-fate transitions and discover whether various aspects of animal developmental gene regulation, e.g., the use of repressive histone modifications in cell-type-specific gene regulation, are also utilised in choanoflagellates. I will also examine the cis-regulatory landscape in S. rosetta and use both molecular and functional assays to examine the types of cis-regulatory elements present, and particularly if enhancer elements are used to regulate gene expression. Finally, I will test the role of selected chromatin regulators in temporal cell differentiation and cell-type-specific gene expression. This ground-breaking work will be the first to study these processes in choanoflagellates, thus pioneering the comparison between definitive cell differentiation, seen in a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Influence of stratosphere-troposphere exchange in a changing climate on atmospheric transport and oxidation capacity', explaining its relevance to EU policy priorities under Fifth Framework.

assistant: **Impact**

Problems to be solvedSTACCATO is a comprehensive study of stratosphere-troposphere exchange (STE) processes and their effect on atmospheric chemistry. STE is a key factor controlling the budget of ozone, water vapour and other substances in both the troposphere and lower stratosphere. Earlier studies of STE have concentrated primarily on the flux of air or trace constituents across the tropopause alone. Shallow exchange events are indeed partially reversible in nature and only produce compositional changes in the tropopause region. However, deep STE events are largely irreversible and have a highly significant and lasting impact on atmospheric chemistry through a substantial body of the atmosphere, even down to the earth's surface. Up until now, the importance of STE for the ozone budget relative to photochemical ozone formation from natural and anthropogenic precursor emissions, including those from aircraft, has remained uncertain. A comprehensive description of STE, which STACCATO seeks to provide, is thus a vital component for understanding the chemical composition of the atmosphere and its consequences.Scientific objectives and approachSTACCATO is undertaking a first detailed investigation of STE mixing of stratospheric and tropospheric air. Meteorological processes under investigation include the creation of fine-scale structures by chaotic advection, radiative decay of tracer filaments and mixing through turbulence in the free troposphere. The non-linear effect of this mixing on chemical processes is addressed with a box model as well as with a global model. The impact of STE on the oxidizing capacity of the troposphere, relative to other factors, is examined with two global chemistry models coupled to climate models. The fate of aircraft emissions is being addre
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Biocompatible flexible polymer alloys based on polyesters from renewable resources for mass- consumer applications involving contact with human fluids and tissues' to be submitted under Fifth Framework.

assistant: **Abstract**

The massive consumption of crude fossil fuel both as energy source and as raw material supply result indeed in environmental penalties incompatible with the concept of sustainable growth in a modern society. In this context, production of fine chemicals through their natural synthesis by fermentation of excess agricultural products or agro-industrial sub-products is a very appealing alternative compared to their conventional production from petrochemical sources. In particular, the synthesis from natural resources of polymers which could be blended to Poly (vinyl chloride), PVC, for production of polymer alloys with properties comparable to elasticised PVC grades, thus leading to a total replacement of phthalate PVC elasticises nowadays heavily contested from both medical and environmental points of view specially in highly-demanding applications such as blood and plasma bags, medical tubing or toys, will be an extremely successful development due to the very high volumes of productions involved and the resulting safety improvement for these highly-demanding applications, and it constitutes the overall objective of the present project.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Deciphering the molecular mechanisms of apoptosis-induced cell proliferation' (MECHAIP).

assistant: **Concept**

Apoptosis-induced proliferation (AiP) is a recently discovered biological process that apoptotic cells are able to stimulate neighbouring cells to undergo additional proliferation. Studies in several model organisms including mammals have suggested that AiP is evolutionary conserved, and may have relevance to both tissue regeneration and tumourigenesis. Intriguingly, caspases, the proteases that normally execute apoptosis, have been implicated to play key roles in AiP through activation of their downstream growth signals. However, the underlying mechanism of AiP remains elusive. Systematic approaches are thus in need to dissect the regulatory network of AiP. By taking advantages of the developing Drosophila eye as an epithelium composed of both proliferating and differentiating tissues, our previous work has revealed that distinct mechanisms of AiP exist in tissues with different developmental potentials. In the proliferating tissue where cells are actively dividing, the initiator caspase (Caspase-9 like) coordinates apoptosis and AiP. While in the differentiating tissue where cells have exited the cell cycle, the effector caspases (Caspase-3 like) activate cell cycle re-entry. Built upon this finding, the goal of the proposed project is to obtain a comprehensive understanding of AiP. To achieve this, we have developed three sensitive and unique assays for analysis of AiP. By employing these assays together with proteomic approaches and systematic screens, we aim at (1) determining how caspases initiate AiP in apoptotic cells; (2) elucidating how Spitz/EGFR signalling, a growth signal that we identified mediating AiP, is activated; and (3) identifying and characterizing critical kinase regulators of AiP in both proliferating and differentiating tissues. Deciphering regu
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Estimating the effective reproductive rate of M. tuberculosis from changes in molecular clustering rates, to measure the impact of public health interventions on TB transmission' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

Excessive delays in treatment onset limit current tuberculosis (TB) control programs, presenting a major obstacle to the control of the TB epidemic. Increasing case detection, both quantitatively and temporally, is considered a priority, benefiting the patient (reduced morbidity and mortality) and the society (shortened period of infectiousness). These improvements in patient care have a common goal: to reduce transmission and eventually contain the spread of TB within the population. However, assessing reduced transmission of TB proofs to be difficult, as to date molecular tools have not been integrated in mathematical models or in field trials of public health interventions.  Therefore, I aim to develop a model that incorporates bacterial genotyping to follow TB transmission within the human host population, hypothesizing that an effective Enhanced-Case-Finding (ECF) method can interrupt TB transmission. Integration of routine epidemiological and genotyping data with bioinformatics and mathematical modelling provides a novel and powerful approach to understand the key determinants of the TB epidemic, such as the Effective Reproductive Number, and predict the dynamics of TB transmission. I have the unique opportunity to position the present proposal as an added-value study that builds on 3-year Cluster Randomized Trial of ECF that I designed, which is about to be launched in The Gambia in 2012. By applying molecular genotyping methods to bacterial isolates collected from both the ECF intervention- and control arm, I will develop a method to measure and model the impact of ECF on the transmission of TB. This will be the first study of its kind in integrating molecular genotyping data in a TB transmission model as applied to a population level interventional study. The i
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Large QED effects in free space' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

We propose to deepen the understanding Quantum-Electro-Dynamics (QED) effects by investigating theoretically and experimentally various aspects of light matter interactions at the single-atom single-photon level. The work-horse of the experimental work will be a single Barium ion trapped and cooled in front of large aperture optical elements. We will demonstrate efficient coupling between a single trapped ion and its own resonance fluorescence using a very high numerical aperture spherical mirror (from NA=0.4 to NA=1), and investigate the underlying energy shifts, changes in spontaneous emission and atomic motional states. Using this set-up, we plan to investigate the discrepancy between experiment and theory of free space coupling, and anticipate that our experiment will provide solid grounds and motivation for that. We will also implement sideband cooling on the quadrupole transition and measure the vibrational ground state using Electromagnetically Induced Transparency (EIT), which has the potential to yield a quantum-noise-limited read out of atomic motion. Last, collective level shifts and super-radiant effects between two ions will be investigated in a linear Paul trap. We wish to solve issues that quantum technology addresses and deals with both timely subjects such as basic components for quantum networks and light-matter interfaces at a quantum level and fundamental questions of quantum optics.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Optopharmacological brain mapping of autism mouse models' (OPTOFRAX), addressing dissemination and exploitation.

assistant: **Impact**

Manipulation of neurons with light-regulated proteins and drugs has opened unprecedented possibilities for phototherapies for neurological diseases. The host laboratory has developed a powerful new optopharmacological tool: a selective photoswitchable modulator of metabotropic glutamate receptors (mGluR). This drug can be activated or inactivated with light so that its action can be finely patterned in space and time. We aim at developing a novel application for this optical tool: a brain functional mapping technique. We will test it at two different levels of resolution. First, we will map the therapeutic site of action at whole-brain level. Inhibition of mGluR is a promising therapeutic strategy for autism disorders, including Fragile X, but the brain region responsible for this effect is not known. We will inactivate the drug locally by illuminating specific regions of the brain and will identify which region is the target for the therapeutic effect. Second, we will map the physiological contribution of mGluR to neuronal plasticity at the minimal spatial resolution: the individual synapse. Activation of mGluR induces long-term synaptic depression at the level of neurons, but it is unknown if it is also true at the level of single synapses. We will inactivate the drug in a single synapse using laser light and will test whether this specific synapse undergoes structural depression. This project will prove the utility of optopharmacological tools to map the therapeutic and phyisological mGluR function. This original approach could open countless possibilities for scientific, medical, and commercial applications. The project is an excellent opportunity for the Researcher to further develop his knowledge and to position him in unique multidisciplinary environment that wil