RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon Europe proposal for the project 'Structural and mechanistic basis of key intracellular steps of enterovirus infection'.

assistant: **Objectives**

Enteroviruses (EVs) are positive-sense, single stranded RNA virus from the Picornaviridae family. With 106 types, they can cause mild to severe diseases in human, especially children, such as common cold (Rhinovirus), myocarditis (Coxsackievirus B3, CVB3) or poliomyelitis (Poliovirus, PV). EVs are known to remodel the cytoplasm of host cells to create replication organelles allowing efficient viral genome replication. They also hijack the secretory autophagy pathway for replication and viral release. Recent cryo-electron tomography (cryo-ET) work done in the Carlson lab has highlight that PV virions directly assemble on replication membrane through a tethering complex where the tether is thought to be viral ATPase 2C. Moreover, they were able to show one class of autophagosomes is carrying bundles of filament proteins, suspected as filamentous actin (F-actin). My project will address two questions in EV replication: (i) how does the viral protein 2C assist membrane-localized assembly? (ii) what is the identity and role of the filaments in virus-induced autophagy? I will use an integrative approach of cell biology, biochemistry and structural biology and PV and CVB3 will be used as model viruses for this project. I first aim to characterize the viral assembly complex by in vitro reconstitution of the interaction between model membranes, purified 2C, capsid proteins and RNA through cross-linking mass spectrometry (XL-MS) and cryo-ET. By combining focus-ion-beam (FIB) milling and cryo-ET to perform subtomogram averaging, I will study the structure of the tethering complex and the one of the filament proteins found in autophagosomes of infected cells. In parallel, to aid the identification of the filament, I will perform proteomic studies through MS study on isolated autoph
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Accident Information and Driver Emergency Rescue' (AIDER) under Fifth Framework.

assistant: **Objectives**

AIDER proposal addresses the objectives of KA1 (System and Services for citizens) in IST Programme, with the aim of improving the efficiency of road accident management.The project will integrate in an innovative way state-of-the-art technology to reduce casualty consequences through a reliable reconstruction of the accident scenario.The AIDER Concept is based on the direct cooperation between on-vehicle Systems and rescue Control Centres to optimise the effectiveness and operative time of the rescue actions.The System will guarantee an automatic link between involved vehicles and rescue services as well as a support at decision level for accident management. Expertise from automotive and military field will lead to innovative project outcomes. The definition of standard for communication protocols and recommendations will be determinant for the widespread diffusion of the project results.Objectives:AIDER project main objective is the reduction of road accident consequences optimising the rescue management in terms of operative time and effectiveness. To accomplish this task, an on-vehicle System will be developed able to automatically send to a Control Centre both an emergency call and full information about the accident severity. AIDER vehicles will be equipped with a detection system to monitor the on-board pre- and post-crash environment. A robust communication system, based on the integration of cellular and satellite aided links, will be provided for information exchange between vehicles and rescue Centres. Communication protocols will be defined to be recommended at European level. SW tools will be implemented to allow the accident reconstruction at Control Centre level, to support the operators in the selection of the most suitable rescue action. Relevance will
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'High value-added chemicals and BIoreSIns from alGae biorefineries produced from CO2 provided by industrial emissions', explaining its relevance to EU policy priorities under Seventh Framework.

assistant: **Impact**

BISIGODOS aims to address the production of valuable algae derived chemicals, amino acids and high added-value bio-resins for coatings, printing, food and hair care and adhesives applications, starting from algae biomass fed directly with CO2 from industrial emissions (cement, steel factory, thermal power plants, etc.) as a raw material that is cost-effective and renewable. The process is assisted by solar radiation, nutrients and sea water microalgae. This approach is based on the technology developed by the Partner Biofuel Systems (BFS) to produce bio-oil. In order to develop such technology, several innovative approaches are proposed:- New algae strains production optimization and CO2 energetic balance improvement.-Optimization of photo-bioreactors- Study and adaptation of separation of algae components based on hybrid technologies.- Production of algae derived chemicals for surfactants applications and amino acids for food applications- Production of bio-based resins from algae based fatty acids and bio-oil aromatic moieties.Similar studies have been carried out at laboratory level to obtain a broad range of algae derived chemicals, however BISIGODOS’ project aims to work at semi-industrial scale using the BFS industrial photo-bioreactors facilities. Results obtained at this scale, under a well controlled process, will permit to validate the lab scale results and to develop new ones (mainly in the bioresin field) gaining a real knowledge of the industrial-market possibilities that the microalgae technologies offer and contributing to define the roadmap of the technology
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'atomistiC approacHes for plasmOnic Photo Induced phenomeNa', explaining its relevance to EU policy priorities under Horizon Europe.

assistant: **Impact**

Plasmonic catalysis is the field of chemistry that studies chemical reactions catalyzed by surface plasmons. By exploiting their unique properties, it offers enhanced reaction rates and selectivity. From a theoretical point of view, describing plasmonic catalysis is particularly challenging, because many mechanisms take place simultaneously. Such complexity and the limitations of current theoretical methods hinder a comprehensive understanding of the phenomenon. In fact, current models struggle to capture the interplay between reaction dynamics, nanostructure response, and plasmonic decay, often oversimplifying critical factors such as shape, size, and atomistic features of the plasmonic substrate.CHOPIN aims to bridge the current gaps in the modeling of plasmonic catalysis, by integrating state-of-the-art techniques from theoretical chemistry, condensed matter physics, and quantum electrodynamics. To this end, I will introduce novel, fully atomistic multiscale methodologies to describe the key steps of plasmonic catalysis: adsorption and activation of the reactants upon plasmon photoexcitation, reaction pathways, and desorption of products. The models will permit quantifying and rationalizing the effects of non-uniform spatial plasmonic fields, plasmonic decay, and hot carrier injections on chemical reactions catalyzed by plasmonic structures of any shape, size, and composition.The developed methodologies will be carefully validated and tested against selected pilot applications, focusing on complex nanoreactors with unique features such as highly localized electric fields demanding a fully atomistic description of the system. By demonstrating the robustness, effectiveness, and versatility of the proposed approaches in modeling plasmonic photo-induced phenomena, CHOPIN
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Development of the Cephalochordate amphioxus, Branchiostoma lanceolatum, as a new model for regeneration' under Seventh Framework.

assistant: **Concept**

While the ability to regenerate lost body parts or organs is legendary in invertebrates like echinoderms, chordates-and mammals in particular-have quite limited regenerative ability. The exceptions par excellence are salamanders, which can regenerate lens, limbs and tails. Unfortunately, vertebrate models of regeneration are costly to maintain, suffer from necessary ethical considerations, and are often plagued by duplicated genes, hindering functional analyses. The invertebrate cephalochordate amphioxus is the most basal chordate. It is characterised by a prototypical genome, ancestral chordate morphology, and lacks the whole-genome duplications characteristic of vertebrates. With sequencing of the genome, it is therefore an excellent model for evolution of developmental mechanisms from an invertebrate ancestor. Until now, there has been no convincing evidence of regeneration in a cephalochordate. However, the candidate’s preliminary data indicate that amphioxus can regenerate all structures of the tail, including neural tube, notochord and muscle. The purpose of the proposed research is to develop the European amphioxus, Branchiostoma lanceolatum, as a new regeneration model. The ojectives are: 1. To characterise proliferation and stem cells during regeneration; 2. To evaluate whether the signalling pathways responsible for vertebrate regeneration are conserved in amphioxus, specifically TGFb and Wnt; 3. To determine whether matrix metalloproteinases are implicated downstream of these pathways during tissue remodelling; 4. To develop electroporation or explant culture methodologies to knock down gene function; 5. To assess the level of conservation of regeneration mechanisms in deuterostomes by comparing with echinoderms. The workplan will include bio-informatics, in
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Dynamics, noise, and coupling in gene circuit modules' in the context of a Seventh Framework call.

assistant: **Objectives**

Cells must integrate output from multiple genetic circuits in order to correctly control cellular processes. Despite much work characterizing regulation in these circuits, how circuits interact to control global cellular programs remains unclear. This is particularly true given that recent research at the single cell level has revealed that genetic circuits often generate variable or stochastic regulation dynamics. In this proposal we will use a multi-disciplinary approach, combining modelling and time-lapse microscopy, to investigate how cells can robustly integrate signals from multiple dynamic genetic circuits. In particular we will answer the following questions: 1) What types of dynamic signal encoding strategies are available for the cell? 2) What are the benefits of dynamic gene activation, whether stochastic or oscillatory, to the cell? 3) How do cells couple and integrate output from diverse gene modules despite the noise and variability observed in gene circuit dynamics?We will study these questions using 2 key model systems. In Aim 1, we will examine stochastic pulse regulation dynamics and coupling between alternative sigma factors in B. subtilis. Our preliminary data has revealed that multiple B. subtilis sigma factors stochastically pulse under stress. We will look for evidence of any coupling or interactions between these stochastic pulse circuits. This system will serve as a model for how a cell uses stochastic pulsing to control diverse cellular processes. In Aim 2, we will examine coupling between a deterministic oscillator, the circadian clock, and multiple other key pathways in Cyanobacteria. We will examine how the cell can dynamically couple multiple cellular processes using an oscillating signal. This work will provide an excellent base for Aim 3,
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Understanding HIV-specific B cell function and viral immunogenicity' (2014-2020).

assistant: **Concept**

I will establish how abnormal B cell behaviour contributes to the generation of HIV neutralising antibodies by studying B cell biology: Previous studies show that broadly neutralising HIV antibodies are the most highly-mutated human antibodies ever isolated, with unusually long binding loops and polyreactivity, suggestive of changes to B cell tolerance and germinal centre selection mechanisms. These remarkable antibodies develop only during HIV infection, concurrent with immune dysfunction, not following vaccination. Crucially, the cellular processes whereby these antibodies arise need to be elucidated and I hypothesize that doing so using RNAseq, flow cytometry and imaging B cell activation will answer why only a minority of HIV+ individuals develop neutralization breadth. Moreover, B cell dysfunction is associated to a range of malignancies and autoimmune diseases and, while much has been learnt from model antigens in mouse models, HIV infection provides a uniquely well-characterised human model to study B cell regulation. Another fundamental question is whether strain-specific antibodies are a stepping-stone to breadth, or a detour that must be eliminated in vaccination. I have shown that the best serum neutralization activity induced via stabilized HIV envelope protein immunization predominantly targets a strain-specific glycan hole, similar to many early responses observed in infection before broad neutralising activity develops. Therefore, I will determine whether non-neutralising and strain-specific antibodies affect the development of breadth by isolating a range of antibodies raised during viral infection from individual donors and comparing binding affinities, clonal ontogenies and B cell receptor activation. Defining the impact of competition between antibody
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Promoting international scientific cooperation between european scientists and their colleagues in russia by means of a user-friendly information system elaboration' will create measurable scientific, societal and economic impact aligned with Fifth Framework.

assistant: **Impact**

Elaboration of a user-friendly information system based on the existing server of the Siberian Information/Consulting Centre for R&T Corporation is aimed at expanding the Network disseminating information on opportunities of international cooperation open for Russian scientists. Current problems of lack of information, Internet traffic and partner search are to be solved using Internet technologies as well as E-mail communications and distribution of paper and electronic copies of newsletter for the regions having no Internet access. Reviews of major tendencies and priorities of EU R&T policy and actual information will be presented (with abstracts in Russian and option of downloading information packages) to promote involvement of Russian researchers EU R&T programmes. A database of scientists interested in cooperation will be composed to introduce a robotic system delivering differentiated acknowledgement on changes on the server.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Sixth Framework proposal on 'Simultaneous five-axis hard milling for highest precision'.

assistant: **Concept**

European tool and die making SMEs are facing a steady loss of competitiveness in their markets, since existing manufacturing technologies cannot fulfil the increasing demands on part quality. These companies are eagerly seeking for new technologies to enhance the economic production of high-quality tools and dies. The main objective of this project is to develop a five-axis hard milling process for highest precision. This new technology will enable end-users to produce high-quality products in less than 62% of current times. This will provide them with a substantial lead over their overseas competitors. The project will encompass the optimisation of a milling machine by applying light-weight parts and the enhancement of milling technology for high-hard materials by process monitoring, as well as of cutting tools and tool coatings for high-performance hard milling operations. Moreover, a part measurement system capable of quality control within the machine tool will be developed. Main work packages are:-development of a light-weight carbon-fibre spindle box and integration into the milling machine-development of a novel in machine measurement device-optimisation of a clamping device-development of a process monitoring system for small milling tools-enhancement of cutting tool accuracy and coating technology-adaptation of CAD/CAM process chain and specialised tool path strategies-machining technology optimisationBesides contributing to an improvement of the bonds between Member States and new members, the project will encompass scientific, technical and wider societal objectives:- better scientific and technological knowledge about milling of high-quality surfaces on hardened materials- lifetime enhancement of tools and dies, contributing to further knowledge and producti
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'eastern enlargement - western enlargement; cultural encounters in the European economy and society after the accession' goes beyond current state-of-the-art under Sixth Framework.

assistant: **Innovation**

Invoking the mythological figures Castor and Pollux,the DIOSCURI project focuses on current encounters in Europe to predict the convergence between the twin economic cultures of the East" and the "West".The research fields - entrepreneurship, governance and economic knowledge - will be explored in 4 East-Central European countries (the Czech Republic,Hungary, Poland and Slovenia) and in 4 countries of South-Eastern Europe (Bulgaria,Croatia, Romania and Serbia and Montenegro).The selection of the fields is based on the conviction that among the producers of economic culture, businesspeople,civil servants and economists exert a vast influence on the economic performance of the Union and the social cohesion between the old and the new member states.Instea d of relying on a simplistic scheme,in which the "strong Western" culture devours the "weak Eastern" one,the Consortium expect to find a great variety of lasting cultural hybrids in economic behaviour.Thus, Eastern Enlargement will be studied in conjunct ion with its neglected counterpart, Western Enlargement. East-West encounters will generate a complex dynamics that includes cultural gaps, strategies to bridge the gaps,and compromises between cultures.In studying them,DIOSCURI will go beyond the world o f the acquis to discover forms of cultural coexistence which are hard to regulate ex ante.The project will bring together leading sociologists,political economists,historians,anthropologists and social policy experts in Central and Eastern/ Southern Euro pe.It will result in a set of field reports,country studies,a comparative analysis and policy recommendations for future accession rounds.The project co-ordination will be provided the Center for Policy Studies at the Central European University,in Budap est.Scientific l