RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Seventh Framework proposal on 'Characterizing the oncogenic properties and target genes of the splicing factor protooncogene SF2ASF'.

assistant: **Innovation**

Cancer is a multifaceted disease involving numerous mutations in many tumor suppressors and oncogenes. Elucidation of the molecular basis of cancer has been and continues to be a major goal of biomedical research in the past few decades. Previous evidence suggests a connection between alternative splicing and cancer, and the activities of many oncogenes and tumor suppressors are modulated by alternative splicing. We found recently that the splicing factor SF2/ASF is a potent proto-oncogene, capable of transforming immortal and primary fibroblasts when slightly overexpressed. We found that SF2/ASF is upregulated in a set of human tumors and SFRS1, the gene coding for SF2/ASF is specifically amplified in some breast tumors but not in normal breast tissue from the same patient. Moreover, we identified several endogenous splicing targets of SF2/ASF, among them a novel oncogenic isoform of the mTOR substrate, S6K1, which is essential for SF2/ASF-mediated transformation. Based on these findings, we propose to study the detailed mechanisms of SF2/ASF-mediated transformation in cancer. We will dissect the specific functions of SF2/ASF required for its activity as an oncoprotein, through a structure-function analysis of its specific domains, using deletion mutants that are impaired in specific biological processes. We hypothesize that the splicing activity of SF2/ASF is essential for its transforming activity. Thus, we wish to identify and validate the regulated alternative splicing targets of SF2/ASF. We will identify these splicing targets on a genome-wide scale, using an exon array recently developed by Affymetrix. These experiments will shed light on the molecular mechanisms by which SF2/ASF is upregulated in human cancer and can lead to transformation. The analysis of a nov
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Fourth Framework proposal on 'Diesel engine simulations aiming to reduce emission levels'.

assistant: **Innovation**

Objectives Future stringent legislation on emissions from diesel engines means a great challenge to the engine manufacturers. The fundamental combustion processes in the diesel engine have historically been considered too complicated to model. However, recent developments of models in combination with the very rapid computer development now make calculations possible with a sufficient degree of accuracy. In the project a model for the simulation of emissions from diesel engines will be developed. Effects caused by fuel injection rate shaping, heat transfer and by exhaust gas recirculation, EGR, will all be taken into account. The model will be carefully validated experimentally as well as numerically. Technical Approach The emission model will be based on a special formulation of the flamelet model called Representative Interactive Flamelets, RIF. The RIF model requires as input time-resolved numerical data of scalar fields from a 3-D flow and combustion calculation, and is then able to calculate emissions of soot and NOx for the combustion cycle under study. Also emissions of unburned hydrocarbons and CO can be calculated. The flamelet calculations will employ a few hundred elementary chemical reactions. The developed tool for emission calculations will be applicable to a wide range of engine operation conditions. First validation will be done in simple" combustion systems, combustion cells. Further validation will be based on the results from well defined engine experiments, which will include variations of the injection pressure and injection rate shape. When a working combination of codes has been developed, participating car manufacturers will validate the combustion/emission simulation tool by comparing with experimental results using their own engines and operati
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Ecological Archaeologies of the Afrotropics' (EcoArch) targeting Horizon Europe.

assistant: **Abstract**

The growing climate crisis and threats to biodiversity have alerted the scientific community of the need to develop a clearer understanding of what has driven landscape changes and how these have impacted biodiversity and ecosystems. The lack of observationally constrained quantitative estimates of those impacts have led to large differences in the scenarios currently used for climate simulations. Nowhere is this more pressing than in the Afrotropics—a biogeographical region of nearly one billion people who are among the most vulnerable to the effects of global climate change. EcoArch brings together expertise in archaeology, geosciences, evolutionary ecology, and land cover modelling to synergistically address fundamental questions about the relationship between humans and the environment over time by documenting landscape changes in the Afrotropics over the last 6000 years. Combining new analytical tools across large landscapes with long occupation histories, we will exponentially expand the quantity and quality of archaeo-ecological data at five geographically diverse sites in Africa and Arabia. Additionally, we will build and logistically support a large regional network of scientists trained and committed to collecting harmonised palaeoecological data. We will conduct state-of-the-art vegetation and fire modelling to determine the relative importance of climate, wildfire, and human activities on landscape changes in the Afrotropics over the last six millennia. These efforts will result in robust local-, regional- and continental-scale land cover and climate reconstructions with a greatly improved spatial and temporal resolution The precision of the datasets we generate will significantly augment inputs to the Earth System Models used to predict future climate chang
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Seventh Framework proposal on 'Determinants of the switch from R5- to X4-tropic virus in HIV-1 infected individuals'.

assistant: **Concept**

HIV-1 variants utilizing CCR5 (R5) are predominant during early infection, emergence of CXCR4 (X4) strains typically occurs later. Although R5 to X4 switch is strongly correlated with increased rates of disease progression, it remains largely unknown which factors are causing the switch. Based on our preliminary data we postulate that both host (immune activation) and viral factors (target cell affinity) are required for switching.A) We hypothesize that high levels of immune activation predispose for a coreceptor switch.This will be longitudinally analysed in the Amsterdam Cohort. In parallel, the occurrence of R5-X4 switching will be investigated in African Cohorts with high TB prevalence inducing significant levels of immune activation.B) Our observation that CCR5-inhibitor therapy results in rapid outgrowth of pre-existing X4-variants without affecting the viral setpoint suggests direct competition between R5 and X4-using variants. We will study if increased target cell affinity of the X4 virus facilitates the coreceptor switch. This will be analyzed with the affinofile assay to determine relative envelope affinities for CD4 and CCR5 or CXCR4 between R5 and X4-variants from the same patients and the absence and presence of factors influencing co-receptor switch as identified in part A will also be tested. Finally relative envelope affinity for its different binding partners (CD4 and the coreceptors) will be verified with solubilized coreceptors using surface plasmon resonance.This study will provide important insights into determinants of coreceptor switching andultimately in our understanding of HIV-1 pathogenesis.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Fourth Framework proposal for the project 'Mutant b3 integrins identified in glanzmann's thrombasthenia patients as model receptors for the study of integrin biogenesis, intracellular trafficking and signal transduction'.

assistant: **Objectives**

Integrin aIIb,B3 is the major platelet fibrinogen receptor and plays an important role in haemostasis by mediating platelet aggregation and thrombus formation. B3 integrins are also involved in a variety of pathological processes including thrombosis, tumor invasion and metastasis, and have become major targets for drug development. Inherited molecular defects in the aIIb or ,B3 subunits have been identified in Glanzmann's thrombasthenia patients, and shown to be responsible for defective platelet aIIb,B3 receptor function. The aim of this project is to stably express autofluorescent, GFP-tagged wild type and mutant aIIbB3 receptors in mammalian cells, in order to investigate their biosynthetic pathway and intracellular trafficking by 3 colour confocal microscopy as well as biochemical analysis of the posttranslational processing. We hope that these studies will contribute to a better knowledge of the structural requirements necessary for integrin aB dimerization, posttranslational processing, intracellular trafficking, recycling and signal transduction, and provide some clues for the understanding of the reduced surface expression of aIIbB3 in GT type II patients. Identification of structural domains necessary for receptor transport and surface expression could lead to the development of a new generation of therapeutics aimed to specifically inhibit ,B3 integrin intracellular processing pathways in a way similar to those characterized for mutant B3 integrins in GT patients. Training content (objective, benefit and expected impact) As integrins mediate a large variety of pathological processes, the identification of structural domains involved in integrin subunit-subunit interactions, intracellular transport and integrin affinity modulation is extremely important for th
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Diffusion in polymer matrices: a quantitative mapping between matrix heterogeneity and non-Gaussian statistics' will create measurable scientific, societal and economic impact aligned with Horizon Europe.

assistant: **Impact**

At the heart of this project is the study of transport phenomena in soft matter systems, a field that has seen large and fast development over the last decades due to its fundamental role for many biological, industrial and technological applications. The combined effort of experimentalists, theorists and computational physicists has unveiled recurring deviations from the so-called Brownian Motion (BM). The latter dates back to the 19th century and represents the canonical model for the study of diffusion in fluids. The two central properties of BM are the linear growth with time of the mean squared displacement and the Gaussian distribution of displacements. The main motivation behind this project is the fact that more and more studies have proved that non-Gaussian diffusion appears to be the standard rather than the exception when dealing with disordered soft matter systems. Analytical and numerical works have demonstrated that non-Gaussian diffusion emerges due to the presence of different kind of heterogeneities in the systems. Then, this project aims at providing a quantitative description of the spatio-temporal environment heterogeneities leading to non-Gaussian statistics in soft matter systems. More specifically, the research will focus on the diffusion of colloidal nanoparticles through polymer matrices. At first, a detailed study to quantify and characterise the polymer matrix heterogeneities will be carried out. Then, state-of-the-art data analysis technique will be employed to provide a full characterisation of the tracers diffusive dynamics in polymer matrices. Finally, a direct mapping will be established between matrix heterogeneity and the emergence of non-Gaussian statistics in the tracers dynamics. By bringing together concepts from polymer physics, tr
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Solubility, fluid-melt-mineral partitioning, and diffusion of sulfur in natural silicate melts' (1998-2002).

assistant: **Innovation**

The solubility, diffusion and partitioning of sulfur in silicate melts as a function of temperature, pressure and volatile fugacities will be investigated. The data are important to constrain degassing behavior of the Earth and the evolution of atmosphere.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Wider dimension for best practice in engineering and manufacturing' (WIDEBEAM) under Fourth Framework.

assistant: **Objectives**

The objective of WIDEBEAM is to deliver advanced electronic communication of engineering, commercial and production information to manufacturing SMEs.WIDEBEAM focuses on Electronic Data Interchange (EDI) in a supply-chain between customer, supplier and sub-contractor. The main goal is to produce a methodology and implementations for information exchange that meet the needs of the consortium members and can be integrated with current IT systems. This will be disseminated to manufacturing SMEs throughout Europe, using multimedia technologies to overcome cultural and learning barriers, inherent in organisations where the IT culture is not present and personnel are not trained in its use. The work will be undertaken by ten manufacturing SMEs many of whom already share business relationships and communication problems. They will be supported by best practice co-ordinators (BPCs) from the UK with expertise in IT and data communications for SMEs, assisted by local co-ordinators in Spain and Bulgaria. The BPCs will be responsible for evaluation of needs and solutions ,a best practice database and training of manufacturing SME staff and managers. They will also undertake dissemination by a variety of means with the assistance of a specialist multimedia authoring partner and relevant European and national associations.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Automated software testing for enhanced reliability in execution' to be submitted under Fourth Framework.

assistant: **Abstract**

The objective of the ASTERIX project is to determine how greater attention to system-level testing can improve software product quality whilst reducing overall costs as measured across the full software development lifecycle (including extended warranty periods).The resources, measured both in terms of cost and elapsed time, which have to be allocated under normal circumstances to system-level testing mean that there is usually a strong temptation to economise in this area. We believe that this situation will improve significantly only if a high level of automation can be brought to such testing. ASTERIX will therefore provide the resources needed to establish whether such an approach, in conjunction with strong higher management support, will lead to real benefits on a major representative project.THE EXPERIMENTThe baseline project chosen for this experiment is the Envisat-1 Monitoring and Control Facility (MCF) project. This 2.8 MECU development is being performed to tight budget and schedule constraints, and can benefit directly from the application of the techniques being proposed within ASTERIX.The ASTERIX experiment will determine whether the additional effort supplied in the system testing phase is repaid in terms of higher product quality at a reduced overall price, compared with the measured quality on other similar projects performed by Anite Systems for major clients. This reflects the overall goal to demonstrate that the approach proposed will result in real gains both for the customer (through receiving a better quality product, allowing him to reduce operational costs) and for Anite (through reducing the overall lifecycle costs).EXPECTED IMPACT AND EXPERIENCEWe see the results of ASTERIX as being having a wide potential application, and we welcome the oppo
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Cyclopropane Synthase Enzymes as Biocatalysts for Green Cyclopropanation Reactions' demonstrating excellence, impact and implementation quality for a Horizon 2020 call.

assistant: **Abstract**

The cyclopropane ring is an important structural motif present in many pharmaceutical compounds and widely used as reactive building blocks in the synthesis of chemicals. Many synthetic strategies to access cyclopropanes have been developed to date, most of which typically require the use of potentially explosive diazo-carbene compounds as substrates. Even recently proposed enzymatic transformations, exploiting cytochromes P450 and myoglobin, require the use of stoichiometric carbene co-substrates, thus narrowing their industrial applications. The CycloproBio project aims to explore cyclopropane synthase (CS) enzymes as a potentially mild method for biocatalytic cyclopropanation. Cyclopropane synthases are SAM dependent transferase enzymes able to catalyse cyclopropanation of unsaturated phospholipids in bacterial membranes. Even if CS are common to most bacteria, many questions on factors affecting their substrate selectivity remain unanswered. Moreover, despite their potential, CS enzymes have not been explored yet as potential biocatalysts. This project will initially characterize the cyclopropane fatty acid synthase (CFAS) from E. coli which has been cloned, expressed and purified in preliminary work. The catalytic properties of the E. coli CFAS will be studied through the development of an appropriate activity assay, the investigation of its substrate specificity and the study of its physio-chemical properties. To broaden the study, genome mining for other CS enzymes from different bacterial species will be carried out to gain insight into the factors which affect the selectivity of these enzymes. Finally, new CS enzyme variants will be generated through rational mutagenesis of putative substrate binding residues. The data generated will provide fundamental informa
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Boosting the market deployment of safe, effective and sustainable innovations for soil improvement from bio-waste, towards regenerative soil systems' (BIN2BEAN).

assistant: **Innovation**

BIN2BEAN will support cities in their transition towards regenerative soil systems by promoting innovations for soil improvement from bio-waste with a value-based approach. The project will implement 3 Living Labs, as pilot city-regions, to follow a multi-actor and participative approach. In each LL, after mapping local contexts in terms of material and monetary flows, a tailored evaluation framework to demonstrate the safety, environmental and socio-economic performance of soil improvers will be co-designed and implemented, through field testing on experimental sites, feasibility studies and choice experiments. The data obtained will feed into a pilot scoring system, that will be co-developed and validated during the project, to help cities selecting the most effective solutions adapted to the geo-spatial context, i.e. inner city, urban-rural fringe and the wider market. The highest scored solutions will be selected for the development of innovative and tailored business models. The latter will match to stakeholders’ willingness-to-adopt (circular) implementation packages, e.g. collection schemes, cooperation networks, infrastructures and fee structures. The pre-market processes will be monitored through Techno-Economic Assessment (TEA). Finally, based on all previous results, local, national and EU policy roadmaps will be drafted, including waste charging policies and citizen awareness campaigns in the city-region, that will be piloted in LLs. All this will feed into a PDCA (Plan, Do, Check, Act) approach, enabling cities to create a continuous value-based improvement loop towards regenerative soil systems.BIN2BEAN will support local waste management with the creation of 40 start-ups specialising in the soil improvers value chains. This will help to reach Europe's 203