RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Targeting the essentialome of radiotherapy-resistant cancer', explaining its relevance to EU policy priorities under Horizon 2020.

assistant: **Impact**

More than 50% of the cancer patients undergo irradiation as part of their cancer treatment. Although radiotherapy (RT) significantly contributes to cancer cure, local therapy resistance and the subsequent emergence of distant metastasis remain major obstacles for its success. The molecular mechanisms underlying tumor cell-intrinsic RT resistance are ill-defined. It is therefore crucial to better define these mechanisms and identify new vulnerabilities of RT-resistant tumors in order to decrease the current annual cancer mortality of >1.3 million persons in EU member states alone.In the TETHER project, I will address the problem of RT resistance by synergizing the power of genetic essentiality analyses with unique mouse models and organoids that we have established. We recently found that members of the shieldin and CST complexes are essential for tumor cells to survive irradiation, while causing PARP inhibitor resistance when lost in BRCA1-deficient tumors. Based on this unexpected finding, I have started a new line of research to dissect the RT ""essentialome"". As I show with the discovery and functional characterization of ERCC6l2 as a novel DNA repair factor in this network, the technology we have in place is perfectly suited to tackle this question. In addition, we will apply distinct CRISPR/Cas9-based tests to map the functional interactome of genes that are essential for RT resistance. To follow the plasticity and RT escape of tumor cells in vivo, we have also developed innovative model systems. Similar to the situation in cancer patients, we observe that residual cancer cells in our mouse models escape the deadly effects of RT by local resistance or metastasis formation. Thus, these models provide a unique opportunity to explore and target RT escape mechanisms.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'A nanovaccine Approach for the treatment of Pancreatic Cancer', explaining its relevance to EU policy priorities under Horizon 2020.

assistant: **Impact**

A Nanovaccine Approach For The Treatment of Pancreatic Cancer By Multicomponent Immuno-Modulation: Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer deaths in men and women and still fatal in over 90% of patients. It is characterised by its extremely aggressive nature where it is also responsible for the highest mortality rate compared to other major cancers, resulting in excess of 250,000 deaths worldwide per annum. Current state-of-art therapies for advanced PDAC including chemo- and/or radiotherapy, despite extensive efforts, have met with only limited success.Surgery is only applicable for those with early stages of the disease, or to relieve symptoms, if the cancer is blocking the bile duct or the bowel. There are two major reasons for the resistance of PDAC to conventional therapy. Firstly, PDAC has a very defining hallmark, where an abundance of stromal content is present in the tumour microenvironment (TME) to form a physical and biochemical barrier. Secondly, during progression of the disease, the body's immune system is hijacked to support the proliferation of the cancer. New approaches, such as immunotherapy, are therefore needed where it has already shown promise in overcoming many aspects of this resistance. Immunotherapy has the potential to treat minimal residual disease after pancreatic resection (surgery) as well as for metastatic and non-resectable PDAC. Our objective for this projectis to bring together a multidisciplinary and intersectoral group to develop novel vaccine approaches, including use of multiple immunomodulating components.


**Keywords:** nanovaccines, immunotherapy, personalized nanomedicine approaches , image guided, surgery, molecular imaging
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Towards constraining the pillars of our cosmological model using combined probes' under Horizon Europe.

assistant: **Concept**

Despite the observational successes of the Lambda Cold Dark Matter (ΛCDM) cosmological model, a number of its key ingredients remain unknown. Imminent surveys promise to yield unprecedented constraints on these components, thus making precision tests of ΛCDM a first-time reality.In PiCo, my team and I will focus on two fundamental questions in modern physics that can only be answered through cosmological observations: What mechanism gave rise to the primordial fluctuations seeding all the structures seen in the Universe today? What is the cause for the Universe’s late-time accelerated expansion? We will address these by developing novel analysis techniques built on the complementarity of cosmological probes and advanced statistical methods. These are designed to harness the rich information contained in non-Gaussian, small-scale features of cosmic fields, while enabling precise constraints on ΛCDM, robust to systematic uncertainties dominating high-precision data. Focusing on two of the most powerful probes of the next decade, galaxy clustering and galaxy clusters, we will constrain the mechanisms driving the accelerated expansion through the first joint simulation-based inference analysis of galaxy clusters selected through the Cosmic Microwave Background (CMB) and weak lensing. In addition, we will derive constraints on primordial fluctuations in a combined analysis of galaxy clustering and CMB lensing angular two- and three-point statistics. Capitalizing on my past work, we will develop these methods using simulations and current data, before applying them to galaxy data from the Rubin Observatory Legacy Survey of Space and Time and CMB data from the Simons Observatory, two upcoming high-precision experiments I am deeply involved in. PiCo will deliver tight constrain
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Promoting Synthetic Polyploid Engineering Commencing Technology' to be submitted under Horizon 2020.

assistant: **Abstract**

Polyploids, which result from Whole Genome Duplication (WGD) events, are thought to have enhanced adaptability which might explain why they are so pervasive in nature and especially in crop plants. Furthermore, polyploids exhibit multiple advantages, from an agricultural perspective, such as often having larger fruits, and being more resilient to like drought and salinity. However, polyploidy requires evolving an adapted meiosis (well documented in natural polyploids) to cope with their additional chromosome sets during meiotic segregation; otherwise, resulting aneuploidies lead to genome instability and fertility problems. Because little is known about these adaptive mechanisms, the meiotic instability displayed by artificially generated (synthetic) polyploids (which lack of an evolved meiosis) cannot be eluded and the use of these synthetic polyploids remains exceptional as a tool to generate novel crop varieties. It is expected that regulating meiotic crossovers to prevent formation of multivalent associations among more than two homologous chromosomes during meiosis would decisively improve segregation and fertility of synthetic polyploids. While compelling, this hypothesis has not been formally tested. ProSPECT aims to address the problem of genome instability in synthetic polyploids by testing the viability of two strategies to artificially induce meiotic adjustments that might improve chromosome segregation using Arabidopsis thaliana polyploid mutants as a proof of concept. To do so I will use two strategies: (a) First, I will use meiotic recombination mutants known to reduce the number of meiotic crossovers to test if this is sufficient to limit the chances for multivalent formation in A. thaliana synthetic polyploid inbreds. (b) Second, I will use polyploid hyb
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Fourth Framework proposal for the project 'The organotypic culture of HPV-transformed keratinocytes: an effective in-vitro model of pre- neoplastic lesions of the uterine cervix'.

assistant: **Objectives**

Cancers associated with potentially oncogenic viruses represent good models for research aimed at developing anti-tumoral vaccines because the viral proteins could be tumor antigens. The most actively investigated example of a tumor-virus is human papillomavirus (HPV), which is associated with more than 90% of squamous carcinoma of the uterine cervix. The generation of an effective protective response in the cervical mucosa is, however, still poorly understood and may be influenced by factors produced by infected keratinocytes and infiltrating immune cells. Because different patterns of synergism or antagonism may be observed between these factors and immune effectors, preliminary studies of immunotherapeutic manipulations should be performed in models approximating the in vivo environment of the tissue of origin. The purpose of this project is to develop a reliable in-vitro human model to test new immunotherapeutic approaches for squamous carcinoma developed on mucosal surfaces and particularly for cancer of the uterine cervix. The organotypic (raft) culture permits cells to proliferate and differentiate at an air-liquid interface on a dermal equivalent support. Normal keratinocytes stratify and fully differentiate in a manner similar to the normal squamous epithelial tissues, while HPV-immortalized and established squamous carcinoma cell lines exhibit dysplastic morphologies similar to (pre)neoplastic lesions seen in vivo. The ability of these organotypic cultures to be manipulated (for example, by integrating immunocompetent cells) may provide a useful tool to investigate the factors contributing to the presence and function of immunocompetent cells within a neoplastic epithelium developed on a mucosal surface. The programme will be a shared activity of eight researc
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Exploring the potential of Iron-based Superconductors' (SUPER-IRON), addressing dissemination and exploitation.

assistant: **Impact**

In 2008 the group of Prof. Hosono discovered the superconductivity in a new compound containing FeAs planes, thus opening the age of Fe-based superconductors (FeSC). Several different phases were rapidly discovered and today the FeSCs show the second high Tc behind the HTSC  and very high critical fields.  These characteristics suggested that FeSCs can be candidates for power application.However, as the recent story of HTSC taught us, the discovery of new superconductors always rises euphoric perspectives concerning their applications, but there are many issues to overcome before actual devices are fabricated.Therefore, within SUPER-IRON we depict the roadmap for exploring and exploiting the potentialities of FeSCs: 1) understanding the fundamental mechanisms and their implication on superconducting properties, 2) control material quality 3) manipulate superconducting properties, 4) assess the potential of FeSCs with respect to other technical superconductors, 5) identify application fields, where FeSCs lead to a step-like change with respect to the current state of the art.To cover this road SUPER-IRON has joined the efforts of the leader groups involved in the investigation of FeSCs throughout EU and Japan.FeSCs  belonging to the different phases and also to new pnictide oxide SC, in form of single crystals, polycrystals, thin films, tapes and wires will be realized by using different synthesis methods. Superconducting properties will be investigated also under high magnetic field and/or pressure and visualization of local electric field and current will be carried out with a number of sophisticated techniques.  This wide variety of experimental activities will be supported by an intense theoretical work including ab-initio calculations and theoretical modelling.The a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Detecting Low-Energy Astrophysical Neutrinos with KM3NeT/ORCA: the Transient Neutrino Sky at the GeV Scale' in the context of a Horizon 2020 call.

assistant: **Objectives**

The era of Multi-Messenger (MM) astronomy has started with the joint observations of gravitational and electromagnetic waves in August 2017, and the announcement of a common source of high-energy neutrinos and gamma rays in July 2018. The ""Low-Energy Astrophysical Neutrinos in ORca"" (LEANOR) project aspires to be the next MM observation, coupling low-energy (GeV) neutrinos with electromagnetic and gravitational waves emitted during short gamma-ray bursts (SGRBs). While the signal/background differentiation is more challenging for low energy neutrinos, they have the advantage to be more abundant than their high-energy counterpart. Produced as a consequence of the accelerated proton flux colliding with the matter surrounding the source, they stand as promising messengers to constrain the local environment and would furthermore be of great help to identify new cosmic sources.To reach this goal, we propose to convert the KM3NeT-ORCA detector, initially designed for neutrino oscillation studies, into a telescope able to study astrophysical GeV neutrinos. A detailed study of the environmental noise will be coupled to a specific selection of ORCA data targeting GeV neutrino interactions. The data sample acquired will then be used to search for statistically significant excess of neutrinos during SGRBs. Three different analyses, based on electromagnetic or gravitational wave observations, will be developed. These analyses will allow to constrain the source environment as well as the hadronic acceleration process taking place in SGRBs.The project will lead to either the first joint observation of GeV neutrinos, electromagnetic and gravitational waves, or the first observational limits set in this energy range for the studied source population. Opening a new line of research in
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Collaborative technologies and environments enhancing the seamless creativity process, leveraging the full European potential' (BRAINBRIDGES).

assistant: **Innovation**

BrainBridges will result in a coordinated strategic European research programme in the area of Collaborative Working Environments (CWE) and enabling technologies. CWE unlocks the potential job creation, productivity, creativity and innovation potential of multi-cultural Europe, and will be a major factor in strengthening and maintaining the Union's future competitiveness in the global marketplace.It is therefore imperative to fully leverage its potential and establish an optimal European research fabric to accelerate CWE development. By uniting a critical mass of key regional and national funding organisations, research and industry players, including a significant amount from New Member States, BrainBridges assures its ability to develop a truly pan-European ERA pilot for CWE.In order to deliver a coordinated European research programme providing a solid common basis for policymaking and programme coordination, BrainBridges will build:- A sustainable community dedicated to knowledge exchange and the establishment of a coordinated European CWE research programme- Suitable networking infrastructure including an IST Collaboration Platform- Leverage of the available scientific excellence and industry relevanceThis will be achieved by systematically gathering and analysing knowledge on a regional, national and European level, regarding the current structure of CWE research funding as well as multi-disciplinary CWE research issues.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Identification and characterization of target genes regulated by KNOX transcription factors in different plant species' under Sixth Framework.

assistant: **Concept**

Animals develop all their organs entirely during embryogenesis. On the contrary, only few organs are established during plant embryogenesis. The development of all the plant organs depends on post-embryonic activity of the shoot apical meristem (SAM) and t he root meristem, which are established during embryogenesis.Plant development and growth depends on the ability of the SAM to renew itself and at the same time differentiate into various organs. These processes are strictly regulated and coordinated by meristematic genes, by local hormonal balance and by abiotic stimuli.In plants, the class 1 KNOX (KNOTTED1-like) family plays an important role in the formation and maintenance of the SAM. KNOX gene expression is first detected as the meristem is established in the embryo and disappears from cells that will give rise to leaf primordia. Over-expression of KNOX genes strongly affects cell fate.Altered cell differentiation produces dramatic changes of the whole plant architecture and leaf morphology. Mutations of some class 1 genes result in seedlings that germinate, but fail to develop any further. KNOX proteins interact with other homeodomain proteins (BELL). In animals the KNOX/BELL complex contains at least 3 subunits. In plants, KNOX and BELL proteins interact and bind the same nucleotide sequence.These findings support the hypothesis that a third interactor must provide binding-site specificity for the differential regulation of KNOX target genes. We propose a multidisciplinary project aimed at identifying KNOX primary target genes and interactors of the KNOX/BELL complex.An innovative application of Chromatin ImmunoPrecipitation (ChIP) modified for gene and protein discovery will be developed. This new approach will allow for the identification of KNOX target genes
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'The role of megafauna in biogeochemical cycles and greenhouse gas fluxes: implications for climate and ecosystems throughout history' goes beyond current state-of-the-art under Horizon 2020.

assistant: **Innovation**

Megafauna play an important role in carbon cycling and fluxes of greenhouse gases. Through consumption and excretion of biomass, megafauna accelerate nutrient cycling, increasing plant productivity and influencing soil greenhouse gas fluxes. Megafauna also emit methane when digesting plants through fermentation. Consequently, megafauna can have wide-ranging impacts on ecosystem functioning and climate. However, methodological limitations in dynamic global vegetation models and research gaps in megafauna ecology hamper our ability to assess these impacts. These gaps include: (i) a limited representation of megafauna in vegetation models; (ii) a lack of large-scale, long-term studies detailing the influence of megafauna on nutrient cycling and greenhouse gas fluxes; (iii) insufficient knowledge  on how the current loss and introduction of megafauna is affecting ecosystems and their services. This project will address these research gaps by first developing a new classification of the effects of megafauna across biomes for present and late Quaternary species. This classification will then be used to simulate megafauna-ecosystem interactions in a dynamic global vegetation model (ORCHIDEE). This new tool will allow to test hypothesis on the effect of past and present changes in megafauna populations on a) plant primary productivity and carbon cycling and b) methane and nitrous oxide fluxes. The proposed project has the potential to greatly advance our understanding of the connection between climate, biosphere, and megafauna. The results will have implication for climate policy and conservation. Strong contributions to climate modelling, ecology, and paleoecology are to be expected. The candidate’s experience in megafauna-ecosystem interactions and ecological modelling, coupl