RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Correlative Super Resolution Imaging of the Collagen Mineralization Process' in the context of a Horizon 2020 call.

assistant: **Objectives**

Bone tissue is an organic-inorganic composite material that provides the mechanical support and protection for our bodies. Its impressive mechanical properties arise from the hierarchical organization of the organic collagen matrix that is mineralized with ultrathin, aligned inorganic crystals of carbonated hydroxyapatite. Despite its importance to the human body, still relatively little is understood about the mechanisms by which collagen mineralization occurs, and what the respective roles are of the collagen and other, non-collagenous proteins (NCPs) in directing this process. This is because the process is complex: there are different stages that occur over multiple length scales, and many different components are involved. So far, studying collagen mineralization has mainly relied on analyses that require sample-altering preparation methods and lack information about the dynamics; or on simplified in vitro systems that do not necessarily represent what happens in the native bone environment. To really understand the role of NCPs in collagen mineralization, we need to study their dynamics and structural interactions with the highest possible resolution and in an environment as close as possible to native bone. I will use a recently developed tissue engineering platform that produces mineralized collagen with the main characteristics of that in bone. This will now allow me to apply gene editing for studying the role of NCPs in situ and in a living system with correlative imaging using super resolution microscopy and cryogenic transmission electron microscopy. My approach will provide unprecedented details on the role of selected NCPs in collagen mineralization. It will significantly impact how bone defects and mineralization are studied, and open the door to new trea
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Modelling Ice Sheet Evolution and Sea-Level Change to Investigate Rapid and Early Antarctic Deglaciation' (MISESCIREAD) targeting Sixth Framework.

assistant: **Abstract**

The climate system has undergone several rapid and large magnitude events in the period from last glacial maximum (LGM) to present day. Two of these key events occur between 15 and 14 cal. kyr BP: the warming of the northern Atlantic region (the Bolling-A llerod warm interval) and a rapid sea-level rise of around 25m in 1 kyr, meltwater pulse IA (mwp-IA). Recent sea-level modelling studies show that equatorial data are more compatible with a melt-source for mwp-IA that includes a dominant Antarctic component [Clark et al., Science, 295, 2438 2002] which could also explain the onset of the Bolling-Allerod [Weaver et al., Science 299, 5613, 2003] whereas, a dominant Laurentide source could result in the shut down of North Atlantic Deep Water formation and a major climatic cooling [Clark et al., Paleoceanography, 11, 5, 1996].The primary aim of this proposed research is to test the feasibility of rapid and large-magnitude deglaciation of the Antarctic ice sheet and consider the possibility of Antarctic melting as a trigger for the Bolling-Allerod warm interval. This will be investigated by utilising both a realistic thermomechanically coupled Antarctic ice sheet model [e.g. Huybrechts, Quaternary Science Reviews, 21, 2002] and an algorithm for predicting glaciation induced sea-level changes [Milne, AGU Monograph Geodynamic Series, 29, 2002]. The effect of climatic and sea level forcings will both be considered as possible triggers to initiate earlier melting of the Antarctic ice sheet.A secondary aim of the research will be to construct an improved model of Antarctic ice sheet deglaciation by using field evidence of ice extent and observations of sea-level changes and crustal deformation from GPS data. The results of this study would have far-reaching impact o n a broad ra
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Understanding mechanisms in inflammation through genetic manipulation of endothelial nfkb in mice' goes beyond current state-of-the-art under Fourth Framework.

assistant: **Innovation**

Research objectives and content During the inflammatory response, cytokines such as Tumor Necrosis Factor (TNF) transiently upregulate in endothelial cells (EC) a set of genes encoding, amongst others, cell adhesion molecules and chemokines that together mediate the interaction of the endothelium with cells of the immune system. Infiammatory gene upregulation seems to be mediated predominantly by the transcription factor NF-kB, and when deregulated, the whole process is thought to play a central role in the perpetuation and chronicity of important inflammatory conditions. In the proposed study, we will test the concept of NF-kB being a central mediator of inflammation and we will attempt to inhibit in vivo inflammatory responses in transgenic mouse lines which will be engineered to overexpress transdominant forms of I kappa B alpha (mIKBa) in EC. To avoid possible developmental deleterious consequences to the physiology of the animal, temporally controlled EC-specific activation of the mIlcBa transgene will be engineered by the use of inducible Cre-loxP methodology. A number of TNF transgenic mouse lines representing models of chronic inflammation already exist in the host laboratory and in combination with the mI-kBa transgenic mice they should be very useful tools in understanding mechanisms of NF-kB interference with EC activation and homeostasis. Training content (objective, benefit and expected impact) The aim objective is to learn the methodology involved in generating genetically modified transgenic mice in one of Europe's leading transgenic research laboratories. This will be of benefit to my career development helping to obtain my doctoral degree and subsequent employment as a post-doctoral researcher. Links with industry / Industrial relevance (22)
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Does Trade Multilateralism have a Future?: Reviving the WTO’s Credibility as the premier Multilateral Trade Negotiating Forum' under Seventh Framework.

assistant: **Concept**

In the present climate of weak economic prospects, the developed countries have seen a growing disenchantment with globalisation and trade liberalisation. Irrespective of their stated interest in enhancing export revenue to bridge fiscal gaps, strong protectionist tendencies are surfacing. Also, the focus seems to be shifting from multilateralism, made evident from the failure to conclude the on-going Doha Round of WTO negotiations and the recent spurt in FTA negotiations worldwide. On the other hand, developments since the recent financial crisis indicates that the WTO is yet to lose it’s relevance as the multilateral referee of global trade relations, as least insofar as its rule-making and dispute-settlement roles are concerned. The laudable resilience of the global trading system and WTO institutions notwithstanding, recent experience with Doha Development Round negotiations has highlighted the need for systemic changes in WTO, in the interest of making the organisation more credible and efficient.It is in this context that this project proposes to analyse possible contours of a reinvigourated multilateral trade negotiation system and trade liberalising philosophy, one that can meet the trade challenges and political-economy realities of the 21st century. To do this, the project will look at the contours of a more constructive engagement and leadership by emerging economies (and in particular Brazil, India and China) in the international trade negotiations, especially given that the major industrialised countries have now reduced their leadership in the WTO-led multilateral trade negotiations. The project will also evaluate the acceptability of a developing-country-led negotiating template that might emerge as result. Finally, we would discuss how the WTO could be r
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'From mineral inclusions in zircon to continents: An in situ isotopic perspective on the evolution of the continental crust, the onset of plate tectonics and the development of a habitable Earth' demonstrating excellence, impact and implementation quality for a Horizon 2020 call.

assistant: **Abstract**

The continental crust is the principal record of conditions on Earth during the past 4.4 billion years, yet how it formed and evolved through time remains unresolved. Zircon lies at the core of crustal evolution studies, and yet our knowledge has remained restricted to the geochemical information that can be extracted from this mineral with current techniques. This CoG moves the debate to a different scale analytically, to the scale of mineral inclusions encapsulated within zircons. A key motivation is the recent analytical breakthrough in the microanalysis of minute samples, which was achieved by the PI and the team he led at the University of Bristol, using state-of-the-art instruments similar to those now available at the PI’s host laboratory. The integrated analysis of Sr and Pb isotopes of mineral inclusions, along with the trace elements, U-Pb, Hf and O isotopes analysis of their host zircons, for over 5000 zircons of different ages and provenance, will provide new and different information to that available from the 'zircon only' record – ultimately to i) probe the inferred transition from intraplate- to subduction-related magmatism associated with the onset of plate tectonics; ii) date this transition and its duration precisely in different places; iii) develop a global model of continental crust evolution from the Hadean (i.e. >4 Ga) to the Present, in which the Earth has progressively, or more suddenly, become a habitable planet. These goals will be achieved through:1. Building a worldwide collection of inclusion-bearing zircons with a range of ages and provenance (WP1).2. Evaluating changes in the degree of differentiation of the newly generated continental crust through time, using the Sr isotope record of apatite inclusions (WP2).3. Addressing changes in th
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Genetically encoded reporters and sensors for whole animal imaging using photo-modulated Optoacoustics' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

In vivo imaging of cellular localization and function and visualization of the chemicals that drive life are key challenges for biological research. Current methods limit our insight into this “bigger picture” to either a glimpse (low penetration and small field of view) or a coarse overview (low resolution).Optoacoustic (OA) imaging has the physical means to overcome these limitations, but it lacks the necessary toolbox of genetically encoded reporters and sensors that allow specific probing of localizations and distributions. So far, all efforts to create such a toolbox were impeded by the strong tissue background inherent to OA. Recently, exploiting my longstanding expertise in reversibly switchable reporters I overcame this hurdle by introducing the concept of photo-modulation to OA. Switchable reporters allow modulation of the reporter signal to separate it from the background – making the latter virtually invisible (“Switch2See”). In parallel, the concept was used by a U.S. consortium in vivo, underpinning its validity.Hence, now is the time to combine my expertise in switching, protein-engineering, sensors, and OA imaging to create a toolbox that will allow to follow cells over time, visualize their interactions, and image the distributions of chemicals – all on the scale of the whole live organism with resolutions of 20 - 150 µm. With Switch2See, I will lay the foundations by building dedicated screening infrastructure, use it to develop a range of reporters (multiplexing) and sensors, and develop algorithms to convert the photo-modulation patterns into images. I will demonstrate the revolutionary impact on whole-animal imaging by visualizing localization, dynamics and interaction of immune cells in the tumor micro-environment (TME) and visualize aspects of its
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Virtual design of flexoelectric sensor for electronic skin' (FlexSen), addressing dissemination and exploitation.

assistant: **Impact**

Soft electronic devices are indispensable for the development of artificial skin due to their high stretchability and sensing functionality. Conventionally, to mimic touch and temperature sensing of mechanoreceptors and thermoreceptors, compliant structural design accompanied with signal transduction mechanism such as piezoresistive, capacitive, piezoelectric, pyroelectric sensing have been utilized so far. The design of these pressure sensors requires highly flexible and robust electrical properties of materials as active and supporting components. In addition, strategically engineered micro-structure is employed to enhance the sensing performance. In this spirit, flexoelectric material, which relates strain-gradient and electrical polarization, emerges as a naturally suitable candidates for flexible pressure sensors. The strain-gradient-induced polarization does not only distinguish flexoelectricity from the commonly used piezoelectricity but also widen the choice of electro-mechanical coupling materials, especially lead-free and bio-compatible materials that are crucial for the development of biomedical devices. Furthermore, flexoelectric effect exhibits size-dependent behavior, particularly at sub-micron- and nano-scale so that proper design of micro-structure can open an opportunity to a new type of pressure sensor. Therefore, this project aims to propose a novel design for electronic skin (e-skin) based on flexoelectric effect. Specifically, a comprehensive virtual design framework including simulation, characterization and experimental testing of flexoelectric-based sensor will be employed to evaluate key parameters such as sensitivity, limit of detection, linearity, response time and power consumption.


**Keywords:** Electromechanical, Flexoelectricity, Electro
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'The Fragility, Instability, Ambiguity, and Self-Reflexivity of Images in Roman Art' under Horizon Europe.

assistant: **Concept**

FRAGILE IMAGES fundamentally challenges the dominant notion of images as powerful actors. It moves away from the focus on the affective, persuasive, performative, and immersive image and instead focuses on its fragility, instability, ambiguity, and self-reflexivity. This radically new approach brings together a Visual Studies perspective and a cultural-historical approach and applies them to Roman imagery from the 2nd century BC to the 4th century AD. It has three main objectives:1) A systematic study of pictorial fragility The manifold phenomena of pictorial fragility are divided into three analytical dimensions: temporal fragility, which deals with the material, contextual, and conceptual mutability of images; semantic fragility, which deals with their ambiguity; and ontological fragility, which addresses their self-reflexivity. The case studies aim at elaborating the semantic, aesthetic, but also social potentials of such ‘fragile’ images.2) A new perspective on Roman visual cultureBy means of a comparative approach, the project explores the extent to which manipulated, ambiguous, and self-reflexive images differ in their spatial contexts and in their socio-cultural forms of use. This focus invites a new way of looking at Roman art: The temporality, ambiguity, and self-reflexivity of Roman images are constitutive elements of an innovative, sophisticated, intelligent, and even intellectual visual culture. This perspective moves away from the old view of Roman art as a mere derivative of Greek pictorial concepts.3) A reformulation of image theory in the light of ‘fragile’ imagesThis project will not only challenge the common notion of the power of images, but also transcend the dichotomy of power and weakness. ‘Fragility’ is not considered as an image deficit, but as a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Assisting European SPACE Start-ups in scaling UP' to be submitted under Horizon 2020.

assistant: **Abstract**

The SpaceUp project aims at giving Europe's innovative start-ups in the space sector every opportunity to become world-leading companies. SpaceUp  promotes technology transfer, networking, outreach and matchmaking with business angels, crowd funders, investors, human resource specialists and CEOs from corporates, culminating in six European SPACE ACADEMIES. Exposure to strategic corporate investors is being increased by engaging actively with relevant high tech industries like automotive, robotics. SpaceUp experts offer advice on how to best profit from the opportunities represented by European Public Funds (H2020/SME Instrument, EIB and EIF) and by the European Space Agency. Promising tech start-ups are being guided through already existing services offered like ESA-BICs, EEN, space-related clusters and NCPs. SpaceUp  builds on activities carried out by ESA’s Technology Transfer Programme Office (TTPO) and ESA BICs, and fosters the uptake of space data and services by non-space industrial and service players for further commercial applications. SpaceUp reinforces the spin-out efforts of ESA through cross-fertilization by enhancing connection and networking with non-space technology experts, e.g. in maritime, logistics, land-use and security sectors. It also fosters spin-in activities from these into the space sector.Up to 360 start-ups in total can attend the six SPACE ACADEMIES  taking place across Europe incl. the widening countries. Of these the most advanced 60 start-ups, applying for the SpaceUp coaching services, selected on the basis of the most viable cases (business, products and/or services) by the SpaceUp  evaluation committee, are being invited not only to attend  the  SPACE ACADEMIES. They also get access to all partners’ services and to customized one-to-
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Molecular characterization of insecticide resistance and transmission competence in sand flies, major vectors of leismaniasis' (LeVec).

assistant: **Innovation**

Leishmania, transmitted by sand flies, comes second only to malaria in terms of parasitic causative fatalities globally. Tunisia, as well as the Mediterranean region are endemic for the disease, reporting hundreds of cases annually. In the absence of human vaccines, controlling the population size of sand flies using insecticides is a key component of leishmaniasis control efforts. Nevertheless, the reliance on insecticides has triggered the selection of resistance, which is threatening the efficacy of sand fly vector control programs. The molecular aetiology behind the insecticide resistance status of sand flies, unlike other insect vectors, remains largely unknown. The aim of LeVec is to unravel the mechanisms that underpin resistance to pyrethroid insecticides in sand fly populations, collected in Tunisia. I will use comparative transcriptomic analysis to identify genes differentially expressed in resistant individuals and functionally validate their role  using state of the art in vitro (biochemical assays) and in vivo (transgenesis and CRISPR genome editing) approaches. The outcomes will substantially improve our ability to track the phenomenon of sand fly resistance in the field and design mitigation strategies. Furthermore, LeVec aims to identify genes that determine the ability of sand flies to transmit the Leishmania parasites. For that I will focus on the sand flys midgut, the site where the complete development of the Leishmania parasites takes place, and perform comparative expression analysis, using a unique collection of 12 sand fly species with different degrees of vector competence. Silencing of candidate genes followed by experimental infections will allow me to validate their role in Leishmania transmission. The outcomes of this analysis will help the