RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for '3D Fire LABoratory' demonstrating excellence, impact and implementation quality for a Horizon 2020 call.

assistant: **Abstract**

This project develops a simulation strategy aiming to create a 3D virtual fire lab that can model radiative transfer in open landscape scale vegetation fire. The end objective is to help improving fire monitoring Earth Observation (EO) products. It builds on an initial system designed by the Experience Researcher (ER) during a previous European Space Agency project, and the coupling of models of fire spread, atmospherics dynamics and radiative transfer developed by the hosts. It takes opportunity of (a) the wide experience of the beneficiary host organization in fire monitoring and modeling, (b) recent efforts conducted by host partners to improve atmospheric representation in  radiative transfer model and fire effects in atmospheric model, and (c) the ER’s experience in the fire remote sensing community. Fire disturbance is parameterized in large scale atmospheric modeling (e.g. forecast model) via emission inventory based on EO products. A well-established approach is to use the Fire Radiative Power product (FRP) to estimate total fire energy emission and infer the associated fuel mass consumption and trace gas emission. So far, the conversion from emissive radiative energy to mass consumption is based on a linear relationship that has only been demonstrated for small scale fire and little evidence are currently present to validate it in the context of large-scale fire scenario. The simulation strategy proposed here aims to setup a tool able to study energy transfer in large-scale fires that will help us understand the roles of the flames and the plume to eventually evaluate their sensitivity in the fire emission FRP retrievals. While project results have potential high application in the atmospheric community, the training organized with the host and the two partners
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Targeting the Oncogenic Function of Myc in vivo' goes beyond current state-of-the-art under Horizon 2020.

assistant: **Innovation**

The transcription factor Myc plays a central role in tumourigenesis but was deemed undruggable due to it being an essential protein. However, recent proof-of-principle studies in mice using a dominant negative allele of Myc demonstrated the dependency of established tumours on Myc function and showed that mice tolerated Myc inhibition to a degree that allowed tumour regression. In line with these observations my group found Myc to regulate distinct sets of genes at low, physiological and high, oncogenic levels, because promoters differ in their affinity for Myc. This notion implies the compelling possibility to specifically target the oncogenic functions of Myc.TarMyc aims to address four key questions required to bring this new concept from bench to bedside. Firstly, TarMyc will estimate the therapeutic window of Myc inhibition in vivo by expressing shRNAs against Myc in mice with established solid tumours. Secondly, TarMyc aims to identify in vivo Myc target genes crucial for tumourigenesis. Thirdly, this proposal aims to elucidate the role of Myc’s differential promoter affinity in untransformed cells. Analysis of published gene expression datasets revealed Myc binding to low-affinity promoters during the process of tissue regeneration. Thus, by characterizing the regeneration programme induced by Myc we hope to gain further insight on the therapeutic window of Myc inhibition and assess potential side-effects in a Myc-targeting anticancer therapy. Fourthly, we aim to develop strategies to interfere with the oncogenic functions of Myc by (i) developing a novel class of drugs that reduce Myc’s cellular concentrations, and (ii) by testing the therapeutic potential of Myc target genes by inhibiting their function in tumour models.Taken together, TarMyc takes on the chall
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Fourth Framework proposal on 'Determining the co2 flux in the Atlantic ocean and its relationship to plankton activity'.

assistant: **Concept**

Research objectives and content The oceans have a fundamental role in global climate change. Due to human activity, the concentration of CO2 in the atmosphere is increasing and there is a paucity of knowledge on the fate of atmospheric CO2. The aim of this project is to better document and understand the CO2 distribution in the At- lantic ocean using data collected by ships of opportunity. An automated CO2 system has been installed on two ships of opportunity, the RMS St Helena, sailing from Cardiff to Cape Town on a 6-8 week schedule, and the RRS James Clark Ross, sailing from Grimsby (UK) to Montevideo (Uruguay) twice a year. The project will focus on the tropical region and subtropical gyres to esta- blish relationships between the components of the planktonic system and the partial pressure of CO2. Algorithms will be developed to link the CO2 con- centration and ocean color. Training content (objective, benefit and expected impact) The objective of the training is to link remote sensed parameters and ocea- nic CO2 concentration. It will allow to extrapolate shipboard measurements to basin-scales and then provide a more accurate estimate of the CO2 flux in the Atlantic ocean. Such an approach can then be used to refine the carbon budget for the global ocean. Links with industry / industrial relevance (22)
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'ElectroChemiLuminescence doctoral network for early sepsis diagnosis' (ECLectic) under Horizon Europe.

assistant: **Objectives**

Sepsis and bacterial infections are leading causes of death in hospitals with more than 500 thousand casualties yearly. The early infection diagnosis as well as early microbial treatment are of great importance. However, effective treatment is hampered by misuse of antimicrobial drugs, leading to resistant bacterial pathogens, high risks of infection recurrence and patient mortality as well as increased medical costs. Thus, there is a need for fast and reliable sensors systems for diagnosis of sepsis reducing the time-to-report from several days or weeks to hours, facilitating the early delivery of appropriate anti- bacteria therapy.ECLectic network will address the worldwide problem of sepsis and bacterial infection by developing the next generation of sensing platforms, providing entirely new diagnostic paradigms. ECLectic will develop, optimize, and assess novel biosensors based on electrochemiluminescence by combining different nano- and microstructured materials and fluidic devices. ECLectic will ultimately result in novel fast and reliable point-of-care devices with applications of bacterial infection analysis, monitoring of disease recurrence, and progression. It will close the gap between innovative research in the field of nanostructured materials and their application in biosensing technology.ECLectic will create a Center of Excellence in Europe for the training of young researchers in the research and development of sensing platforms for bacterial infection and sepsis. By training, ECLectic will provide highly skilled European researchers for academic research in biodetection, materials, microfluidics and electrochemistry as well as for the European biosensor industry. ECLectic will generate a flexible cluster of excellent young researchers ready to tackle th
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Molecular and metabolic mechanisms underlying mitochondrial dysfunction' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

Mitochondria are essential organelles that carry out critical metabolic functions within the cells. Electron transport chain (ETC) defects occurring from mitochondrial disease mutations compromise cellular fitness and survival. This biochemical failure is thought to underlie pathologies associated with mitochondrial dysfunction. However, the precise metabolic processes, signaling pathways and compensatory responses resulting from a defective mitochondrial ETC that drive these fatal disorders are not entirely understood. Although diminished ATP production has been considered a hallmark of mitochondrial dysfunction, I hypothesize that other metabolic failures such as disturbed redox hemostasis due to accumulated levels of NADH can be equally detrimental. Moreover, which cell types contribute the most to the disease and whether disease-carrying cells negatively impact the function of its surrounding wild-type neighbors or distant organs remain poorly characterized. Here I propose to develop a holistic understanding of the molecular and metabolic components that contribute to cell and tissue deterioration in the context of ETC dysfunction. Our central goals are: (1) To identify novel uncharacterized genes controlling function and integrity of ETC complexes and supercomplexes (2) To define how ETC dysfunction impacts cellular metabolism with special focus on redox-sensitive pathways that might be altered as a consequence of imbalanced NAD+/NADH ratio (3) To elucidate brain-specific non cell-autonomous signaling that mediates maladaptation to mitochondrial dysfunction. In sum, MitoCure puts forward an ambitious but feasible program with the purpose of yielding novel information about genes, metabolic pathways and signaling mechanisms underlying the pathophysiology associated
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Prospecting natural lignin biodiversity towards unlocking value-added bioactive motifs and molecules' (PROSPLIGN) targeting Horizon Europe.

assistant: **Abstract**

Global terrestrial plant biodiversity remains a largely untapped source of natural bioactive compounds. In fact, valid sources are even “hiding in plain sight”, yet not “within reach” due to lack of effective technical solutions to unlock their potential. In this context, the PROSPLIGN consortium has developed an innovative bioprospecting approach that uses cutting-edge and complementary chemical and enzymatic methods, supported by statistical analysis and coupled with high-throughput detection methods, to enable the discovery of bioactive molecules from lignin, one of the fractions of the most abundant biopolymer on Earth (lignocellulose). Lignin “chemical biodiversity” will enable PROSPLIGN to cover three target markets (pharmaceuticals, cosmetics and fragrances) with a combined market of >€1.4 trillion. The project will use lignin derived from 9 different species and investigate at least 3 different chemical approaches and 10 different enzymes to obtain up to 1000 mixtures of lignin-derived compounds, and then pass a progressively narrower “hit validation funnel” in industrially relevant environments, being screened via up to 17 different bioactivity assays. Sustainable production routes for at least 2 of the most promising compounds per sector, directly or following derivatisation, will be proposed. Compared to traditional approaches, PROSPLIGN's bioprospecting avoids animal cruelty, expensive exploration efforts (no novel animal/plants discovery), disruptive extractions (seeking “hidden gems” in an abundant but underexploited “biomass mine”), and directly targets the liberation of readily testable bio/chemical-functionality present in plant material, superior to other approaches relying on “manipulation-intensive” microbial strain culture or “extrapolation” from DN
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Controlling Reactive Intermediates in Biocatalysis through Computational Design Tools' to be submitted under Horizon Europe.

assistant: **Abstract**

Enzymes are nature’s most efficient catalysts, enabling complex reactions with remarkable speed and selectivity. However, their scope is limited by evolutionary pressures, restricting their utility in synthetic and industrial applications. While enzyme engineering has achieved significant advancements, major challenges remain. Directed evolution has been transformative, but it is labor-intensive and relies on suitable starting scaffolds. Computational enzyme design is promising complementary strategy, however, its potential remains largely unrealized as designed enzymes often require extensive laboratory evolution, underscoring critical gaps in our understanding of enzyme catalysis. This project addresses these limitations by developing an integrative computational framework for designing enzymes capable of catalyzing new-to-nature reactions involving highly reactive intermediates. It proposes a transformative approach by combining a holistic understanding of enzymatic catalysis with cutting-edge computational tools inspired by nature’s evolutionary principles. The innovation lies in leveraging enzyme electrostatic preorganization and active site confinement to control and enhance catalysis in complex reactions involving high reactive intermediates. By harnessing conformational dynamics and introducing targeted sets of mutations, the project aims to create confined, polarized environments that stabilize reactive species and guide them along desired pathways. Integrating mechanistic insights with electrostatic preorganization and advanced AI-tools for protein structure prediction, this approach enables precise identification of protein sequences optimized for modern biocatalysis, including radical and photocatalytic processes. Beyond advancing fundamental understanding,
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Tracing the evolution of alpha-proteaobacterial genomes', explaining its relevance to EU policy priorities under Sixth Framework.

assistant: **Impact**

The microbial world remains largely unexplored. The goal of this project is to study microbial genome diversity and understand how the genomes of microbial pathogens evolve, using the alpha-proteobacteria as a model system. The alpha-proteobacteria are a bacterial subdivision of much interest from both a medical and an ecological standpoint. Many species establish chronic host-infections, with higher vertebrates (e.g. human, cattle and rodents) as well as with plants and insects. Well-known alpha-proteobacterial infections include trench fever, cat-scratch disease (Bartonella sp.) and formation of tumour-like structures in host tissues, such as in plants by Agrobacterium tumefaciens. Moreover, other alpha-proteobacterial species are known to establish tight symbiotic interactions with plants, such as is the case with the formation of root nodules that are involved nitrogen fixation. Furthermore, they represent a genetic group that is predominant in the environment, as suggested by environmental shotgun genome sequencing of the Sargasso Sea. Because of the broad diversity in genome sizes and structures, the alpha-proteobacteria offer an excellent model system for studies of the forces, mechanisms and rates whereby bacterial genomes evolve. At the date of this writing, more than 20 alpha-proteobacterial genomes have been completely sequenced, ranging in size from 1.1 to 9.1 Megabases, and many more will be available in the near future. The aim of this project is to study the evolution of the alpha-proteobacteria by analyzing their genomes, and as such gain insight in how specific alpha-proteobacterial lineages have evolved into the present-day pathogens and (endo)symbionts. This will be achieved by reconstructing ancestral alpha-proteobacterial genomes and to specifically
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Factor XII and the contact system: cross-talk between thrombosis and inflammation' to be submitted under Seventh Framework.

assistant: **Abstract**

Combinations of proinflammatory and procoagulant reactions are the unifying principle for a variety of disorders affecting the cardiovascular system. Factor XII (FXII, Hageman factor) is a plasma protease that initiates the contact system. This system starts a cascade of procoagulant and proinflammatory reactions via the intrinsic pathway of coagulation, and the bradykinin-producing kallikrein-kinin system, respectively. The biochemistry of the contact system in vitro is well understood, however its in vivo functions are just beginning to emerge.We have previously demonstrated that FXII is essential for thrombus formation while being dispensable for hemostatic processes that terminate blood loss. Challenging the dogma of a coagulation balance, targeting factor XII protected from cerebral ischemia without interfering with hemostasis. In contrast, excess FXII activity is associated with a life threatening inflammatory disorder, Hereditary angioedema. We recently have identified platelet polyphosphate (an inorganic polymer) and mast cell heparin as in vivo FXII activators with implications on the initiation of thrombosis and edema.The current investigations will explore roles of the FXII-driven contact system at the intersection of procoagulant and proinflammatory pathways using genetically altered murine models. We aim to understand activation, regulation and functions of the system for ischemic heart disease, vascular leakage in Hereditary angioedema, allergic airway inflammation as well as procoagulant reactions driven by bacterial infections in skin and lung.A key aspect of this proposal will be analysis of common principles, interactions and cross-talk between coagulation and inflammation, to identify novel therapeutic targets. Elucidating the FXII-driven contact syst
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Implantable Microsystems for augmented liver perfusion' (IMALP) under Fourth Framework.

assistant: **Objectives**

Development of an implantable blood pump system capable of delivering a flow rate of 1 to 2 litres/minute against a back pressure of up to 50 mm Hg with a maximum diameter of 8 mm and maximum length of 30 mm.Development of efficient sensing and control functions to the implanted motor/pump system and transmission of power and data by 'leadless' electromagnetic transmission through sub-cutaneous tissue. Development of a compact, efficient, high energy, rechargeable battery which can be implanted just below the skin.In vitro liver test models for testing the implantable pump system.The IMALP project develops a microsystem incorporating a blood pump to improve functions in patients with liver cirrhosis. The proposed system will comprise a fully implantable electric micromotor driven micropump incorporating 'smart' flow, pressure and temperature sensors based on CMOS technology. By employing state-of-the-art ASICs, sensing and electrical processing of sensor signals will be performed on ICs installed within the motor/pump housing, which will be small enough to be implanted into the portal vein of the liver using minimally invasive procedures.