RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Nitrogen assimilation in prochlorococcus - cloning, sequencing and characterization of glna. Study of gs regulation' will create measurable scientific, societal and economic impact aligned with Fourth Framework.

assistant: **Impact**

The aim of this work is to clone, sequence and characterize the glnA gene from the Prochlorophyte Prochlorococcus, encoding for glutamine synthetase (GS) and to study the regulation of this enzyme. An heterologous probe will be used to screen a Prochlorococcus gene library already constructed, for glnA cloning. After sequencing, it will be characterized by comparison with glnA from other Cyanobacteria, organisms very related with Prochlorococcus. Experiments on GS regulation will focus on two aspects : nitrogen source and light changes. The time course of GS activity, GS protein and glnA transcript levels will be measured by spectrophotometric assays, quantitative immunoelectrophoresis and Northern blotting (using the cloned gene as a probe), respectively. Nitrogen regulation will be studied using different nitrogen sources, including nitrate, nitrite, ammonium and nitrogen starvation. The effect of some inhibitors of enzymes involved in nitrogen assimulation (methionine sulfoximine, azaserine) will be analyzed as well. On the other hand, different experiments concerning regulation of GS by light will be performed, with particular attention to the effect of changes from high to low irradiance, and darkness, in the presence or absence of glucose. The proposed project include the use of very different techniques, from classical biochemistry to molecular biology, which ensure the training in a wide range of modern laboratory methodology, to ensure a good background for future research.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Novel, switchable chiral stationary phases' in the context of a Sixth Framework call.

assistant: **Objectives**

Function and properties of molecules depend on their three dimensional (stereo) structure. The structure is determined not only by types of atoms and bonds but also by its symmetry and chirality. The last phenomenon concerns the molecules (stereoisomers) w hich remain in the relation object-its mirror image and thus are not superimposable. Stereoisomers exhibit usualy dramatical differences in their biological activity. One stereoisomer can be active drug, the second (mirror image) can possess no activity or be even harmfull (story of Thalidomide). Selection of the appropriate stereoisomer offers therefore benefits of better therapeutical profile of drug and the reduction of the dose and side effects.There is tremendous demand for developing new tools for sep aration stereoisomers either for preparative (production) or for analytical purposes (research, medical studies, quality control). Desiging of new chiral stationary phases for chromatograpic separation meets this demand. The goal of this project is the syn thesis and evaluation of novel, chiral stationary phases. These exhibit many unique features: - are switchable - can adopt their shape to the shapes of analysed molecules (selectands), - contain Cinchona alkaloid molecules which are known for numerous appl ication in organic synthesis as catalysts, resolving agents and single chiral phases. They are also widely available, relatively cheap and non-toxic, - are of C2 symmetry (which is very rare in the common chiral selectors, but very succesfull in the field of stereoselective synthesis and molecular recognition), - have many binding sites crucial for molecular recognition. My preliminary studies showed that compunds of this group efficiently recognize either achiral or chiral molecules. Based on these results and
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Green Cities for Climate and Water Resilience, Sustainable Economic Growth, Healthy Citizens and Environments' (2014-2020).

assistant: **Concept**

The frontrunner cities of Manchester (UK), Valencia (Spain), Wroclaw (Poland) and Wuhan (China) and the Fellow cities Brest(France), Zadar (Croatia) and Modena (Italy) are currently inhabited by 12.7 million people and sit at the heart of wider metropolitan areas which are home to 17.8 million people. All cities already experience flooding and heat stress, with projections for these issues to increase due to climate change and ongoing development. Working in complex, resource-constrained urban environments, the municipalities for each city have committed to delivering joined-up, cost-effective, smart solutions to address these and other urban challenges. They recognise that the cities of the future will need to achieve more with less resources and deliver genuine sustainable development that realises a broad range of social, economic and environmental objectives. Each city believes that nature-based solutions (NBS) are a critical part of this approach.GROW GREEN brings the partner cities together on the basis of these similarities but also their differences. Across the 6 European and 1 Chinese City they represent the range of different cities that are found across the world, and the different climate risks that they face. The cities will demonstrate a replicable approach for the development and implementation of city NBS strategies.The outcome will be more than simply demonstrating a methodology that works in the partner cities. GROW GREEN will provide the platform for a step change in the way that NBS are embedded in the long-term planning, development, operation and management of cities around the world. The project outputs will be promoted directly to replication cities globally to encourage them to develop and implement NBS strategies and action. These channels have
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Flair-flow innovation workshops' under Fifth Framework.

assistant: **Concept**

Flair-Flow Europe (FFE) is the EU dissemination network of 24 European countries, disseminating results from EU sponsored food research to She's, Health Professionals and Consumer Groups. Based on this unique dissemination project, this AM proposal is aimed to :1. Strengthen the technology transfer to European She's,2. Improving the dialog between the EU scientists, EC and the She's,3. Improving the conversion of new scientific findings into improved quality and / or innovations based on market demands. The method proposed to achieve the goals disarrangement of 16-18 Innovation Workshops throughout Europe on important food topics related to the Synthesis reports disseminated by Flair-Flow Europe.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon 2020 proposal for the project 'Revolutionary Non-Disruptive DDoS Testing Technology that significantly strengthens resistance to DDoS attacks and provides ongoing visibility on identified DDoS mitigation vulnerabilities.'.

assistant: **Objectives**

Distributed Denial of Service (DDoS) is a form of cyber attack that makes a machine or network resource unavailable to its genuine users resulting in (i) downtime and (ii) a smokescreen for the introduction of viruses or other malware or to steal customer data.  The effective downtime created by DDoS attacks is devastating for organisations that require reliable, non-stop (24/7) online availability – including telecommunication, mass transport, government voting systems, health systems, banking & financial institutions and e-commerce businesses. In 2016, the average effective downtime from each DDoS attack was 17 hours  with an estimated average cost to companies of $250,000 (~€213,000) per hour .  DDoS attacks are becoming easier to launch and the frequency, size and complexity of these attacks are increasing.Although DDoS attacks can be blocked using DDoS mitigation solutions, these solutions currently have limited effectivity.  Effective and well aligned attack mitigation, on the dynamic systems run by organizations, requires continual DDoS testing.  The challenge is that currently all DDoS testing technologies are themselves disruptive to the test environment – meaning that they are restricted to isolated tests at specific time points and require costly dedicated maintenance windows that limit the number of tests which can be performed.MazeBolt Technologies has developed the first ever Non-Disruptive (ND) DDoS testing technology that continuously simulates a wide variety of DDoS attacks without disrupting the tested environments. This offers a step change in the level of information and visibility on companies’ DDoS mitigation vulnerabilities.  Our revolutionary DDoS Analyser runs exponentially more tests, continuously 24/7, with real time analysis of identified vul
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Modelling cancer in Caenorhabditis elegans' (CANCEROMICS).

assistant: **Concept**

Cancer is a complex disease that results from accumulation of diverse alterations in the genome. There are more than 300 genes identified as “cancer genes” although it is estimated that there are many more involved in cancer development that could be significant during tumorigenesis and putative targets for drugs therapies. Several scientific groups are performing a comprehensive sequencing of genes in cancer cells but these studies lack of functional validation. Since cancer requires the combination of several defects in the genome, an organism amenable for genetics and functional genomics studies as Caenorhabditis elegans would provide the ideal platform to identified those fatidic combinations. I plan to take advantage of C. elegans mutants and a “RNA interference” (RNAi) library to uncover functions and pathways of the following C. elegans genes: A) SWI/SNF complex subunit ZK1128.5 baf-60: The SWI/SNF complex is conserved from yeast to humans and acts in chromatin remodelling to regulate gene expression. baf-60 mutants present extra proliferation at particular lineages. B) Claspin, F25H5.5: Human Claspin acts as a DNA damage checkpoint through the activation of chk1 protein kinase. chk-1 also acts as a DNA damage checkpoint in C. elegans. I have preliminary results in C. elegans pointing to a novel role of Claspin in genome stability. C) Spliceosome (or splicing-related) components rsr-2 and lsm-2: I have recently published that rsr-2 and lsm-2 act in the RAS pathway that determine the vulval cell fate in C. elegans (Ceron et al, 2007). This observation and others suggests functional relationships between spliceosome-related genes and cancer. The discovery of functional networks for these genes will contribute to create a “Functional map for cancer genes” that will
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Robust MINFLUX microscope for improved system stability and accessibility' under Horizon 2020.

assistant: **Concept**

MINFLUX super-resolution microscopy provides a 3D spatial resolution down to 2 nm combined with millisecond temporal resolution in living cells. MINFLUX has the potential of revolutionizing structural cell biology by measuring dynamic functional changes of protein machines. Currently, however, access to MINFLUX is very limited because the implementation of custom-built MINFLUX microscopes is complex, and commercial systems are very expensive. The objective of this proposal is to develop a MINFLUX system with simplified optical scanning systems, which makes the system robust, affordable, and easier to build, to provide increased accessibility to a wider range of researchers. To this end, we aim to replace the 3D scanning systems with a combination of spatial light modulators and fast switching of multiple optical fibers, without compromising its imaging speed and achievable resolution. We will demonstrate the performance of the new MINFLUX system on biological systems to showcase the system capability.The research will take place at the group of Dr. Jonas Ries at the European Molecular Biology Laboratory, a leading group in developing new approaches in single molecule localization microscopy and application in biology. This highly interdisciplinary project will strengthen my expertise in advanced optics, software programming, electronics, and biology. It will have an important impact in the field of structural cell biology by enabling direct measurements of dynamic structural and functional changes of protein machines in living cells. This fellowship will establish me as a recognized researcher in the field and will be the optimal basis to start my own research group.


**Keywords:** fluorescence microscopy, super-resolution, single molecule localization, MINFLUX
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'Science Education CUrriculum REsearch'.

assistant: **Objectives**

The EU lags behind its global competitors when it comes to the number of  MST graduates. A special effort is required to close this gap.  The overall aim of the SECURE project is to make a significant contribution to a European knowledge-based society by providing relevant research data that can help policy makers to improve MST curricula and their implementation throughout the EU in order to prepare children from an early age on for future careers in MST, whilst at the same time making MST more accessible and enjoyable for  all children so that they will keep a vivid interest in science and technology, and understand the importance of their societal role.SECURE will focus on the 5 – 13 age group, because the foundation for a revived interest in MST can best be laid at an early age, when children are most susceptible for the wonders of the world that surrounds them.A rigorous research programme conducted by the SECURE consortium will scrutinise and compare current  MST curricula for pupils aged 5, 8, 11 and 13  in the member states  as they are intended by the authorities, implemented by the teachers and perceived by the learners. The instruments used to this end will consist of a transnational comparative screening instrument for MST curricula, of teacher and learner questionnaires and of a lesson observation instrument.The cornerstone of the valorisation strategy of the research outcomes will be the direct and active involvement of a transnational expert group of research and curriculum development institutions that will provide feedback as well as a direct access to policy makers.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'LAY AND SOCIAL SCIENCE DISCOURSES ON IDENTITY, CITIZENSHIP AND MIGRATION' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

The proposed project aims to examine the ways in which the principles used in the social sciences to explain the social world might interact with the interpretative resources that are used by lay social actors to make sense of this world. The project aims to examine this by focusing on the underlying processes of interaction between social scientific and everyday lay discourses: the different ways in which social-scientific discourses are synthesised, how these discourses are filtered back to lay discourses, and how these discourses are taken up by lay social actors. The topics selected to probe these issues are identity, citizenship and migration. There have been global developments in these areas since the 1990s and there has been a proliferation of both social scientific and lay discussions concerning them. The interaction between social scientific and lay discourses will be studied by conducting a systematic review of social science texts on identity, citizenship and migration and by interviewing immigrants and locals in Central Macedonia, Greece. Both sets of discourses will be analysed for correspondence of themes and arguments. The role of policy will also be examined by considering the ways in which policy is manifested in those discourses. The project draws on Critical Discursive Psychology (CDP) and Social Network Analysis (SNA). The project will be of interest to academic users in the sociology of science and knowledge, and in discourse, identity and migration studies; and to non-academic users such as policymakers, local government and non-governmental agencies and local communities and interest groups.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Surveillance of Translation: From Molecular Mechanisms to Roles in Disease' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

Proteostasis is a dynamic network integrating protein biogenesis, folding, trafficking, and degradation to build a functional proteome. Disrupted proteostasis resulting the accumulation and aggregation of aberrant proteins in neurons is a hallmark of neurodegenerative diseases. This project’s overall goal is to uncover molecular mechanisms that maintain proteostasis and protect against neurodegeneration, by taking a fresh look at these processes through the lens of Ribosome-associated Quality Control (RQC), a fundamental translational surveillance mechanism whose defect causes neurodegeneration. RQC targets incomplete proteins produced by stalled ribosomes for proteolysis through two pathways mediated by factors associated with the large (60S) subunit: the E3 ubiquitin ligase, Listerin/Ltn1, and NEMF, which tags the nascent-chains with polyAla-tails that act as degrons sensed by other E3 ligases. Our preliminary data uncover that Ala-tailing modifies neurodegeneration in Ltn1-mutant mice: while Ala-tailing provides initial protection against loss of Ltn1 function, Ala-tailed proteins that evade degradation eventually accumulate and aggregate, contributing to the disease. We will create mammalian cell models, develop methods to study endogenous RQC, and use state-of-the-art biochemical and functional approaches, to: elucidate how Ala-tailed protein accumulation contributes to cellular dysfunction and toxicity (Aim 1), investigate the role of RQC in proteostasis dysfunction and cytotoxicity caused by disease-associated gene mutations (Aim 2), and uncover novel factors that sense ribosomal stalling (Aim 3). The results will lay the foundation for understanding how defects in RQC cause neuronal dysfunction, whether RQC plays a broader role in human disease, and why neurons
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'RegUlation and norM for low sonic Boom LEvels' in the context of a Horizon 2020 call.

assistant: **Objectives**

Nearly 15 years after the last commercial supersonic flight, the quick evolution of technology combined with the emergence of ambitious industrial projects indicate that a second era for environmentally friendly supersonic commercial flights is about to happen.One of the main obstacles remaining on the path to sustainable supersonic commercial flight is the issue of noise, specifically the loud and sudden sonic boom felt by the populations overflown during the entire cruise. The high level of sonic boom produced by supersonic aircraft at the time led to a complete ban of civilian supersonic flights over land in the United States and several other countries.Since then “low boom” technologies have emerged, opening the door to regulatory evolutions.RUMBLE is dedicated to the production of the scientific evidence requested by national, European and international regulation authorities to determine the acceptable level of overland sonic booms and the appropriate ways to comply with it. RUMBLE will not aim at producing a low boom aircraft design but rather the quantified evidence needed to support new regulations.To this end, RUMBLE will associate the leading organizations in supersonic aviation in Europe and Russia, combining scientific excellence, world-class research infrastructures and industrial leadership bearing the heritage from Concorde and Tu-144, with strong involvement in the regulatory bodies.RUMBLE will develop and assess sonic boom prediction tools, study the human response to sonic boom and validate its findings using wind-tunnel experiments and actual flight tests. Extensive dissemination and regulatory activities will ensure that the European considerations are taken into account in the evolution of the international regulation affecting civilian supersonic