RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Structural cell biology in situ using superresolution microscopy' (CellStructure) under Horizon 2020.

assistant: **Objectives**

Supra-molecular protein machineries control diverse cellular processes. Knowing their structural organization is crucial for understanding their function. As classical structural biology techniques are limited in studying such assemblies in their natural cellular environment, there is a critical methodological gap inhibiting a direct link between structure and function. Consequently, the structural intermediates underlying a full activity cycle of a large multi-protein complex have been impossible to visualize. Recent advances in fluorescence microscopy, in particular the development of groundbreaking superresolution microscopy (SRM) methods, can now help bridge this gap. With this interdisciplinary proposal, my group will develop unique and innovative optical, biological and computational imaging technologies to determine the structural organization of multi-protein assemblies in their functional cellular context.We will reach this goal by developing a method to robustly measure the precise 3D arrangements of proteins in supra-molecular assemblies in situ with nanometer isotropic resolution based on supercritical-angle detection and by measuring their absolute stoichiometries with engineered counting standards. We will also develop new data analysis tools to statistically analyze such data, taking into account the functional cellular context measured with correlative superresolution and electron microscopy, multi-color SRM and molecular biology tools. We will apply these new methods to address key questions on endocytosis, a fundamental membrane trafficking process. Our aim is to determine a time-resolved 3D superresolution localization map of the yeast endocytic proteins during the major functional transitions and to integrate these data into a mechanistic model of en
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Biological, physiological, genitical, technical aspects from the mycorrhization between Boletus and forest plant' demonstrating excellence, impact and implementation quality for a Fourth Framework call.

assistant: **Abstract**

In forest ecosystems, tress cannot survive without mycorrhizes. This symbiosis is fundamentally essential as well for the tree as for the fungus. For more than 20 years, the mycorrhizal inoculation of forest tree has had a two fold objective: the enhancement of wood production and edible fungi production. Since 1973, truffle sporophores have been used to inoculate oak seedings which are commercially produced. Paradoxically, despite of the frequency of the Boletus species allowed to get mycelium. Mycorrhizal synthesis has been achieved in controlled conditions in a rather too long period of time (sometimes one year). Despite these positive results, the mycorrhizal association with Boletus and forest plant is neither known nor controlled yet. Indeed, no Boletus fruitbody was able to be obtained after being transplanted. For this, several reasons can be quoted: little knowledge of the Boletus biology, bad control of the physico-chemical conditions of the medium, imperfect struggle against contamination by the spores of other fungi -T.terrestris Hebeloma. This project consists of characterizing the genetic diversity of the Boletus, isolating, selecting and testing the Mycorrhization Helper, determining the nature of the trophic exchanges between the fungi and the forest plants and finally, optimising the mycorrhizal synthesis by varying the conditions of the environment and medium. The results of this project will be used by a development study which will consist of finding a cultivation process of Boletus-plants with an idustrial finality.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'AutoScan – Rail inspection by autonomous systems' (AutoScan).

assistant: **Concept**

There are 215,720km of rail track in Europe, of which 4,300 broken rails are found every year. Broken tracks are the biggest cause of derailments, leading to loss of life and major disruption to services. Maintenance costs for member states of repairing broken rails are around €2bn every year. With the rapid increase in train traffic, train speeds and load carried, there is an urgent need to optimise the maintenance regime and increase reliability of rail infrastructure. Overall integrity and safety maintenance costs in the UK alone are more than €2,276m in 2014, with 20-40% of that spent on tracks (€455m - €910m).AutoScan system(TRL6); an autonomous robotic evaluation system that performs rapid NDT inspection of track, accurately detecting defects, their position and size so that accurate assessment and scheduling of repair work can be made by the network operator.  Current methods or rail track inspection AUTOSCAN will enable more frequent inspections of rail track which will significantly reduce lifecycle costs by enabling more efficient flaw detection and intervention. Operators will not need personnel to walk the track side, therefore improving safety. Overall inspection costs will reduce by at least 15% and probably significantly moreThe aim of this project is to enhance the current prototype from TRL 6 to TRL 9 and develop a system proven in an operational environment suitable for commercial exploitation.AutoScan will enable us to become more competitive within our major target market – Rail network operators and rail maintenance companies. With project development costs of €1,822,055 partly funded by company revenues, we aim to achieve a 2.3% European market penetration, with estimate sales revenues of €41M over the 5 years, with profits of €34.7M, providing an
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'ETH Zurich Postdoctoral Fellowship Program' (ETHFELLOWS).

assistant: **Concept**

ETH Zurich applies for co-funding of its newly established ETH Fellowship program after it has been financed exclusively by ETH’s internal resources in a pilot phase. The program supports incoming fellowships for postdoctoral researchers on a competitive basis. The ETH Fellowship program aims at high-potential individuals primarily interested in following an academic career. Awardees will be offered an ETH employment contract with a standard postdoctoral salary for a maximum of two years. ETH Fellows will be also entitled to an annual individual travel and consumable budget and are expected to take part in the teaching activities of their host group. Furthermore, fellows are encouraged to apply for additional competitive funding. The program is open for all applicants who have obtained an earned doctorate from an institution other than ETH Zurich and the University of Zurich within 2 years of the date of application. The two-year rule can be waived in case of a demonstrated career break. Applications are to be made jointly by the candidate and their host, who must be an ETH Professor and who will act as a supporter and mentor of the fellow. No thematic restrictions apply and, therefore, all research topics of interest to ETH Zurich are eligible for funding. Proposals will be judged primarily on the basis of scientific merit and the applicant’s track record by the ETH Research Commission and external reviewers. The ETH Fellowship program offers an excellent opportunity for experienced researchers to acquire new skills and competencies boosting their careers and helping to attain an independent senior position. Support by COFUND together with an increase of ETH’s own contribution will enable to double the number of trans-national fellowships. This will significantly enhan
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Revolutionary Platform to Decipher Immunogenicity of Tumour Neoantigens- the Ultimate Targets for Future Immunotherapies to Eradicate Cancer' will create measurable scientific, societal and economic impact aligned with Horizon 2020.

assistant: **Impact**

Immunotherapy has revolutionised cancer treatment, providing survival benefits in patients with hard-to-treat tumours. Tragically, these benefits are unevenly distributed. Clinical efficacy varies dramatically between (and within) cancer types, and severe side-effects persist. To address this, new treatment strategies seek the rational design of personalised therapies to achieve full eradication of most cancers. Such a vision can be achieved by inducing immune responses against the ultimate tumour-specific targets: immunogenic tumour neoantigens (iNeoAg). However, a major limitation is the lack of technologies to identify iNeoAg from the thousands of background mutations in tumours. The DECOD-Ag consortium envisions a revolutionary technology realising an unbiased high-throughput transformative immunogenicity profiling platform that, for the first time, uniquely identifies iNeoAgs with the following enabling technologies: i) high throughput screening technology based on random mutagenesis and artificial antigen-presenting cells, to categorise immune recognition triplets (MHC-neoantigen-TCR), define the rules of neoantigen-T cell engagement and the immunogenicity of the neoantigen; ii) robust in silico prediction algorithms to predict neoantigen immunogenicity and neoantigen-T cell pairing; and iii) clinical validation workflow combining multiple advanced immune monitoring technologies. The DECOD-Ag project will be conducted by world-leading, interdisciplinary scientists, with expertise in cancer immunotherapy, bioinformatics, peptidomics, mass spectrometry, immune monitoring, clinical and translational medicine. The DECOD-Ag platform and GENESIS predictor will lead to a new frontier towards developing novel and effective anticancer therapies, with the radical potential
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Development and characterisation of anthozoan cell cultures for application in manne ecotoxicology, conservation and epidemiology.' to be submitted under Fifth Framework.

assistant: **Abstract**

Anthozoan cell cultures will be developed and characterised. In vivo and in vitro exposures will be used to determine the impacts of external stress on anthozoans. These will be related to global issues such as the conservation of coral reefs that are underpinned this symbiosis.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'NEW MOLECULAR APPROACHES FOR IMPROVED VACCINES TO POULTRY DISEASES' will create measurable scientific, societal and economic impact aligned with Fourth Framework.

assistant: **Impact**

The poultry industry is facing increasing problems due to the emergence of progressively more virulent forms of pathogenic viruses, which require increasingly effective vaccines to prevent disease. However, vaccine strains aggressive enough to protect against these new viruses can themselves cause pathogenic effects. The aim of this project is to overcome this problem by identifying the specific peptides within vaccines which cause the strongest vaccine response in animals of particular genotypes, so that combinations of vaccine and host genotype can be developed which maximise both the strength and consistency of protection.There has been rapid progress in understanding the manner in which the immune system recognises and reacts to foreign molecules, and it is now clear that this process depends crucially on the ability of molecules of the Major Histocompatibility Complex (MHC) to present small peptide fragments of proteins to the immune system. Only a small proportion of the peptide fragments present in viral proteins are presented in this way, and animals with different MHC molecules present distinct, very specific, subsets of these peptides. It is already known that chickens of some MHC types respond well to certain vaccines. By systematically extending this information it will be possible to identify animals of similar genotypes in commercial flocks. More dramatically, it will be possible to characterise the particular viral peptides these MHC types present, making it possible to improve vaccines both by increasing the proportion of these peptides in the vaccines and by matching particular vaccines to birds of particular MHC types. By identifying and selecting the MHC types present in flocks it will then be possible to optimise vaccination. Most significantly, this
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'COELIAC DISEASE: UNDERSTANDING HOW A FOREIGN PROTEIN DRIVES AUTOANTIBODY FORMATION' (AUTO-CD) under Seventh Framework.

assistant: **Objectives**

The goal of this project is to understand the mechanism of how highly disease specific autoantibodies are generated in response to the exposure to a foreign antigen. IgA autoantibodies reactive with the enzyme transglutaminase 2 (TG2) are typical of coeliac disease (CD).  These antibodies are only present in subjects who are HLA-DQ2 or -DQ8, and their production is dependent on dietary gluten exposure. This suggests that CD4+ gluten reactive T cells, which are found in CD patients and which recognise gluten peptides deamidated by TG2 in context of DQ2 or DQ8, are implicated in the generation of these autoantibodies. Many small intestinal IgA+ plasma cells express membrane Ig hence allowing isolation of antigen specific cells. Whereas control subjects lack anti-TG2 IgA+ plasma cells, on average 10% of the plasma cells of CD patients are specific for TG2. We have sorted single TG2 reactive IgA+ plasma cells, cloned their VH and VL genes and expressed recombinant mAbs. So far we have expressed 26 TG2 specific mAbs. There is a strong bias for VH5-51 usage, and surprisingly the antibodies are modestly mutated. TG2 acts on specific glutamine residues and can either crosslink these to other proteins (transamidation) or hydrolyse the glutamine to a glutamate (deamidation). None of the 18 mAbs tested affected either transamidation or deamidation leading us to hypothesise that retained crosslinking ability of TG2 when bound to membrane Ig of B cells is an integral part of the anti-TG2 response. Four models of how activation of TG2 specific B cells is facilitated by TG2 crosslinking and the help of gluten reactive CD4 T cells are proposed. These four models will be extensively tested including doing in vivo assays with a newly generated transgenic anti-TG2 immunoglobulin knock-in
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Directly Interfaced And Micro Or Nanostructured Detection Systems' demonstrating excellence, impact and implementation quality for a Fourth Framework call.

assistant: **Abstract**

This project is designed to address the fundamental marriage between biological molecules and electronic transducers to produce measured signals related to the biological event occurring, (eg. catalysis) . The overall objectives of this full project may be summarised as follows: 1) To bring together materials engineers and biological engineers and form a realistic communication forum to enable efficient intellectual transfer leading to technology development, (multi-disciplinary tasking). 2) To investigate the molecular interfacing of enzymes to electronic surfaces with reference to electronic communication, stability, manufacturability and utility in real" analytical environments. 3) To explain mechanistically at the molecular level the interfacial conditions and how the surface structure, chemistry and interactions occurring determine macroscopic results obtained. 4) To manufacture laboratory prototype biological sensors and characterise performance parameters. 5) To be able to reproduce the results at a manufacturing level in a commercial environment, with validation and transfer techniques to other systems within the project as they occur and field testing of the sensors produced. In accordance with the recommendations of the commission a limited number of enzymes will be studied to investigate interfacial coupling of biochemical molecules to electronic surfaces. The project will focus on haem-containing peroxidases, a PQQ cofactor quinoprotein - fructose dehydrogenase and a flavoprotein - alcohol oxidase, building on the preliminary results from the exploratory award. To fulfil the objectives stated the project has been sub-divided into 5 tasks: Task 1: Overall content.Biology. * Enzyme structure evaluation (using molecular modelling and structure software * The de
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Systems Biology as a Driver for Industrial Biotechnology', explaining its relevance to EU policy priorities under Seventh Framework.

assistant: **Impact**

Metabolic engineering is an applied science focusing on developing new cell factories or improving existing ones. Metabolic engineering is an enabling science, and distinguishes itself from applied genetic engineering by the use of advanced analytical tools for identification of appropriate targets for genetic modifications and the use of mathematical models to perform in silico design of optimized cell factories. In recent years, there has been increasing focus on using mathematical models for design. SYSIBIO will coordinate European activities in the field of model driven metabolic engineering and also coordination of activities on other technologies required for state of the art metabolic engineering, e.g. metabolomics and fluxomics. The coordination of activities will involve establishing a database containing metabolic models for different industrially important microorganisms. The database will also contain different simulation tools required for use of these models to identify metabolic engineering targets and use of these models for analysis of omics data. SYSINBIO will also coordinate the further development of techniques required for metabolic engineering, such as metabolomics, fluxomics and identification of mutations in evolved strains. Furthermore, an important part of SYSINBIO will be coordination of education and training in the field of metabolic engineering in Europe.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Multiscale Thermal Management of Computing Systems' (MULTITHERMAN).

assistant: **Concept**

As computing platforms evolve into heterogeneous, 3D-integrated many-cores, increasing performance per unit area (or volume) comes unavoidably with growing power density, which becomes heat, leading to degradation, acceleration of chip aging and increase in cooling costs. Thermal dissipation is difficult to control at the micro-scale, where typically spatial and temporal power gradients are orders-of-magnitude higher than at the macro-scale.MultiThermMan will move beyond the unsustainable worst-case design practices adopted in traditional thermal planning and reactive thermal management. We propose to integrate thermal-aware platform design, thermal control with workload management and shaping in a distributed, multi-scale strategy. This would enable to dynamically adjust the operating mode and active cooling control of each component in complex computing platforms to achieve the highest performance compatible with temperature constraints. The development of a synergistic performance, power and thermal management strategy requires major breakthroughs in several areas, namely architectures, run-time systems, resource management middleware, code optimization tools and programming models. To meet this challenge, MultiThermMan will bring together concepts and techniques from several disciplines: computer architecture and circuits, control theory, combinatorial and continuous optimization. statistical model-building and artificial intelligence.  Results will be demonstrated on physical and virtual prototypes, proving practical applicability and relevance for industrial applications.