RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Symbiont-mediated defense of amoebae against Legionella pneumophila - molecular mechanisms and pathogen ecology' to be submitted under Horizon 2020.

assistant: **Abstract**

Legionellae are opportunistic human pathogens infecting thousands of people annually in Europe. These bacteria are ubiquitous in many natural and man-made water systems, where they survive as free-living organisms in biofilms or intracellularly within a variety of protozoa. Free-living amoebae are the main route for spread and replication of legionellae in the environment, and infection of humans generally occurs via amoebae as vectors. Amoebae are frequently associated with bacterial endosymbionts, and recent data suggests that these symbionts interfere with replication of legionellae in amoebae thereby protecting the amoeba host from legionellae-induced lysis. The aim of this proposal is to further investigate this phenomenon of symbiont-mediated defense in protozoa, and to assess its implications for the ecology and transmission of legionellae. To shed light on the molecular and physiological interactions during infection of symbiont-containing amoebae with Legionella pneumophila, co-infection experiments will be performed and analyzed by state-of-the-art molecular methods including transcriptomics, proteomics and metabolomics, isotope profiling and chemical imaging techniques. Infection experiments will first be performed under controlled laboratory conditions to understand the interaction between amoeba, their bacterial symbionts and L. pneumophila, to analyse the molecular cross-talk, and to determine the mechanism of competition between the bacterial partners in this association. Subsequently, mesocosm experiments simulating environmental conditions will help to understand the impact of bacterial symbionts of amoebae on L. pneumophila spread and replication in the environment. Taken together, the comprehensive analysis of symbiont-mediated defense in amoebae will
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon 2020 proposal on 'Time of damage in classic galactosemia: is prenatal toxicity a determinant factor?'.

assistant: **Innovation**

The TIME project aims to ascertain the time at which the toxicity observed in classic galactosemia begins, particularly if it begins prenatally. Recent advances in the molecular bases of classic galactosemia have taken research a step further towards therapy development. Therefore, it is of utmost importance to elucidate when the damage accurately begins, in order to define the time at which therapy should be initiated so that it can effectively prevent the development of long-term complications.The Galactosemia Research Group (GRG) in Maastricht has recently developed a zebrafish model of classic galactosemia, which is particularly indicated for studying developmental processes. I will use transient embryological- and whole life-knockout galt-zebrafish to analyze damage in target-organs at different stages of pre- and post-natal development and compare them to the wild-type fish, which will allow determining the exact time of damage onset in classic galactosemia.The GRG is a world-renowned multidisciplinary group that will strongly enhance my professional and personal qualifications as a researcher. In turn, I will contribute with my expertise in molecular bases of rare metabolic disorders.This project constitutes a major milestone for this disorder, as it will allow the development of an effective treatment, which in turn will decrease galactosemic patients' requirement for medical assistance and by making them fit to work and to fully engage in the society.Prof. Patrick Cunningham (Trinity College, Dublin) once said: To be a researcher is not just to be in the laboratory, you work for the world, you work for the society. This sentence illustrates what I have always felt to be my purpose as a researcher. Ever since I initiated my scientific career that I am fully comm
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Multilateral adverse selection in industrial economics and general equilibrium' goes beyond current state-of-the-art under Horizon 2020.

assistant: **Innovation**

It is aimed to understand the economic consequences of multilateral private information, and to obtain corresponding policy implications. The focus will be on two types of contexts: (i) oligopolistic firms seeking to constitute a collusive agreement, having private information about their costs and/or the quality of their products; (ii) agents trading in an otherwise perfectly competitive environment, having private information about their preferences and/or the quality of the products that they bring to the market. In the presence of multilateral adverse selection, a small number of firms may not be able to reach a cooperative agreement to divide the market if a firm's willingness to cooperate is interpreted as a sign of weakness by the other firms. Knowing that the other firms are willing to cooperate and, therefore, are relatively weak, if a firm is still willing to cooperate, this reveals its weakness to an even greater extent. As a result of such a cascade, firms may never be able to establish a cooperative agreement. In an otherwise perfectly competitive environment plagued by adverse selection, if an agent purchases a good that he/she cannot distinguish from other goods, the agent should receive the good that is the cheapest among those that he/she cannot distinguish from the purchased good. General equilibrium analysis of such economic contexts can be made possible by using an analytical trick of distinguishing goods not only by their physical characteristics but also by the agent that brings them to the market. The novel insights generated from these investigations, and the understanding of their scope of validity, matter to competition policy in oligopolistic and approximately competitive industries. They can also be used as building blocks for further researc
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Hijacking small hydrophobic protein transport to deliver therapeutic agents to the central nervous system' in the context of a Horizon Europe call.

assistant: **Objectives**

Neurological disorders affect over 540 million individuals in Europe alone, yet effective treatments are limited, primarily due to the challenge of delivering therapeutic compounds across the blood-brain barrier (BBB) and the blood-cerebrospinal fluid barrier (BCSFB). This project aims to develop a novel, virus-inspired technology that utilizes small hydrophobic (SH) proteins, derived from the neurotropic mumps virus to shuttle CNS-active molecules across the BCSFB via the GPR125 receptor. This innovative approach will allow for the effective delivery of therapeutic agents and avoid issues surrounding chemical modification and viral vector packaging of drugs. Building on promising preliminary data, where an SH protein conjugated to Exendin-4 significantly reduced appetite in mice, this project will focus on understanding transport mechanisms of SH-conjugated molecules into the brain. The research will be divided into three work packages (WPs). WP1 will investigate the mechanism of SH-GPR125 transport in vitro using choroid plexus cells and advanced imaging techniques. WP2 will examine the pharmacokinetics and bioavailability of SH-Silicon Rhodamine (SiR) in vivo, determining its uptake in GPR125 knockout and wild-type mice. WP3 will assess the efficacy of SH-enkephalin, a therapeutic peptide targeting the mu-opioid receptor, in an acute pain model, testing its ability to reduce pain perception in mice.This project offers a transformative approach to CNS drug delivery, with the potential to revolutionize treatment for a range of neurological disorders, including Alzheimer’s, Parkinson’s, and ALS. By leveraging the natural ability of neurotropic viruses to cross the BCSFB, we propose a method that bypasses current limitations in CNS drug delivery. The outcomes of this res
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Actomyosin driven force-generation at the egg surface during fertilization' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

The fusion of two specialized gametes represents the starting point of life in sexually reproductive organisms. Despite this fundamental role in the creation of life, the molecular and biophysical mechanisms underlying this process remain largely elusive. It is evident that the dynamic modulation of the egg actomyosin cortex plays a central role during different steps of fertilization including cortical granule exocytosis, Ca2+ signaling and sperm uptake. How this high plasticity of the actin cortex is regulated during fertilization has not been studied in detail. In the past, research of this dynamic process has been limited by live-cell microscopy techniques lacking the needed spatiotemporal resolution. Gamete interaction is not only a very fast process; in mammals, it also occurs synchronously with gamete activation. To overcome these limitations, we propose utilizing eggs isolated from zebrafish that can be activated independently of interaction with sperm. Specifically, we will comparatively characterize the dynamic egg actomyosin cortex properties during fertilization using advanced single-molecule imaging techniques and biophysical tools like optical tweezers. We will specifically address whether the egg actin cortex has mechanosensitive properties that might facilitate gamete fusion, as has been shown for other cell-cell fusion events. It is our goal to identify the basic molecular and biophysical mechanisms underlying fertilization, and the comprehensive quantitative study of actin structures within the egg cortex will establish an essential step in addressing this question.


**Keywords:** fertilization, zebrafish, mechanotransduction, actomyosin cortex, exocytosis, calcium signaling
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Bystanders, Residents, Operators and WorkerS Exposure models for plant protection products' under Seventh Framework.

assistant: **Concept**

The BROWSE proposal is focussed directly and precisely on all the requirements of the call text. Specifically, it will:- Review, improve and extend the models currently used in the risk assessment of plant protection products (PPPs) to evaluate the exposure of operators, workers, residents and bystanders.- Use the new and improved exposure models to contribute to the implementation of Regulation 1107/2009 on authorisation of PPPs, replacing Directive 91/414/EC.- Use the new and improved exposure models to contribute to the implementation of the Thematic Strategy on the Sustainable Use of Pesticides.- Involve all relevant stakeholders and end-users and take full account of relevant gender issues in developing the exposure models and policy tools.The workplan is aligned with these key objectives. In addition, several key cross-cutting themes are established to ensure their consistent and integrated treatment throughout the project. These are: exposure scenarios, volatilisation, transfer coefficients, statistical modelling and calibration, and data management. Key stakeholder groups will be represented on the project Advisory Panel as well as participating directly via consultations and workshops, and in surveys to obtain new data on practices and sociobehavioural and gender factors influencing exposure. Models for key exposure scenarios covering different regions of the EU will be developed in order of priority based on consultation with stakeholders, implemented as user-friendly software, and tested with end-users. Project outputs will be delivered through established networks with end-users in EU and national authorities, national training organisations, the pesticide industry, and relevant trade unions and NGOs. The consortium is superbly equipped to address the projec
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Optimized Fuels for sustainable transport in Europe' (OPTFUEL).

assistant: **Innovation**

OPTFUEL undertakes a first large scale demonstration of the Biomass to Liquid (BtL) production chain from biomass via gasification and fuel synthesis to the final fuel in the consumer car. BtL production will be demonstrated in an industrial size plant environment (15000 t/a). All production chain components from biomass provision up to market introduction of final consumer fuels containing BtL will be optimized and demonstrated including:  The preparation of a feedstock supply concept for a large scale BtL plant of 200.000 t/a, 200 ha of perennial crop cultivation, static and dynamic process modelling for upscaling, BtL production, blending and fuel upgrading techniques, testing of BtL diesel and naphtha blends in advanced EU V engines, vehicles and future engine concepts. Commonly agreed fuel specifications will be derived for BtL-blend fuels. Well to Wheel (WTW) analysis of this pathway with different parameter studies, dissemination of biomass supply concepts and the demonstration of BtL blend fuel in show cars are included.  VOLKSWAGEN has joined forces with Ford and Renault providing test facilities for the new fuels which are produced by Choren Industries, the leading company in biomass gasification for synthetic transport fuels including adapted feedstock concepts. CONCAWE, the research organisation of the mineral oil industry and research institutes IFP and Certh responsible for blending and upgrading BtL ensure a high quality fulfilling the requirements of a premium diesel fuel. Invensys for modelling the production plant, the Indian Institute of Technology for testing BtL blends in the Indian market and SYNCOM a project management enterprise are complementing the consortium. The results of this project are expected to be the basis for a 200.000 t/a BtL produc
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Planning tool for underground heat exchangers' (1994-1998), addressing dissemination and exploitation.

assistant: **Impact**

Underground heat exchangers consist of air-conducting pipes buried in the ground. They are used for pre-heating air during winter time and for pre-cooling during summer. They can often considerably reduce losses associated with ventilation and, if appropriately designed, present an economic extension or alternative to conventional heating and cooling systems.  For the planned New Acropolis Museum in Athens, an underground heat exchanger can reduce the cooling power insummer by about 40 % and the heating power in winter by about 65 %", stated Sebastian Herkel, who is responsible for leading the project. Although a large number of underground heat exchangers have been installed, there is a lack of simple planning tools to provide reliable information and allow the economic and energy potential of this technology to be exploited. On the one hand, underground heat exchangers are simple to install and operate, but make high demands on the design under the economic boundary conditions. The aim of the project is to develop a planning tool, with which the project partners can plan underground heat exchangers reliably and economically."
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Elucidating the development of sexually-dimorphic circuits: from molecular mechanisms to synapses and behavior' (DimorphicCircuits).

assistant: **Innovation**

In sexually reproducing species, males and females respond to environmental sensory cues and transform the input into sexually dimorphic traits. These dimorphisms are the basis for sex-biased phenotypes in many neurological diseases. Yet, complete understanding of the underlying mechanism is still missing. How does the sexual identity impose molecular changes to individual neurons and circuits? What are the sex-specific synaptic changes that occur during development in these circuits? We recently demonstrated a sexually dimorphic dimension of neuronal connectivity: neurons belonging to a shared nervous system rewire in a sex-specific manner to generate sexually dimorphic behaviors. New findings from our lab further reveal a significant difference in the way the two sexes in the nematode C. elegans respond to aversive stimuli. These dimorphic responses are mediated via sex-shared circuits that receive similar environmental input, yet respond differently. Building on our exciting preliminary results, we seek to elucidate how genetic sex modulates neuronal function, neural circuit dynamics and behavior during development. This proposal will pursue three complementary objectives: (i) Map the repertoire of sexually dimorphic avoidance behaviors; (ii) Study the synaptic basis for the development of sexually dimorphic circuits; and (iii) Elucidate the molecular basis of sexually dimorphic neuronal circuits. These mechanisms can only be currently resolved in C. elegans, where the entire connectome of the nervous system for both sexes has been mapped. Using cutting-edge optogenetics, calcium imaging, activity-dependent trans-synaptic labeling, genetic screens and single-cell transcriptome analysis we will shed light on the elusive connection between genes, circuits and behavior.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Acute pancreatitis therapy by RORα activation and FGF21 expression' in the context of a Horizon Europe call.

assistant: **Objectives**

Pancreatitis in humPancreatitis in humans is a cause of significant morbidity and mortality. Acute pancreatitis events have increased worldwide over the last 20 years at an average annual percent change of 3% and the economic burden is growing (1). Treatments for pancreatitis are currently limited. The overall mortality rate in acute severe pancreatitis is 10-15%. Thus, a specific treatment for pancreatitis is of great need. Recent reports suggest that FGF21 can ameliorate acute pancreatitis in mice. FGF21 is a protein which belongs to the fibroblast growth factor family and is predominantly produced and secreted by the liver. FGF21 is positively regulated by the ROR transcription factor. I will assess whether a ROR agonist that we identified by screening compounds that act against Non-Alcoholic Steatohepatitis (NASH) in mice, and which we found to increase FGF21 expression, can be used to treat acute pancreatitis (2). In addition, we will also investigate whether increased FGF21 expression can be used to prevent pancreatitis triggered by Endoscopic Retrograde CholangioPancreatography (ERCP). We established the Caerulein and alcohol induced pancreatitis models. In the Caerulein model we show [unpublished and patented (US Pending Patent 11,865,090)] significant therapeutic effects of a small compound that activates ROR. In this PoC, we intend to explore new compounds of the same family with better efficacy on FGF21 in vivo, and improve the treatment for acute pancreatitis. This study will establish FGF21 as a prognostic biomarker for acute pancreatitis in humans, by plasma ELISA and circulating free DNA. This PoC project has the potential to achieve a breakthrough therapeutic treatment for acute pancreatitis; one that is commercially viable, effective, avoids an immune r