RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Identification of the molecular mechanisms whereby actin and myosin1b shape sorting endosomes' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

Sorting events that occur in endosomes are very important for cellular trafficking of proteins and membranes along the endocytic pathway. The shape of sorting endosomes plays a major role in their function. However, the molecular mechanisms that control the shape of sorting endosomes are far from being elucidated. Motors and cytoskeleton can influence the shape of membranes, especially of high curvature, by establishing tension on membranes. I propose to study the role of an actin cytoskeleton-motor, myosin 1b (Myo1b), a mammalian class I myosin, in regulating the morphology of sorting endosomes. Myo1b localizes to sorting endosomes and when overexpressed increases the formation of tubules in endosomes. I hypothesize that Myo1b induces the formation of membrane tubules by recruiting actin filaments that generates a pulling force on endosomal membranes. The aim of my project is to understand the molecular mechanisms involved in tubule formation mediated by Myo1b in sorting endosomes in mammalian cells. First, I will identify the mechanism by which Myo1b localizes to sorting endosomes. Second, I will establish whether Myo1b recruits actin filaments to sorting endosomes. Third, I will show that Myo1b is required for the formation of tubules in membranes of sorting endosomes. Fourth, I will investigate which sorting events on endosomes are dependent on Myo1b function. These aims employ molecular biology, biochemistry, cell biology and biophysics methodologies in a novel approach to study the shape of sorting endosomes. Ultimately, I wish to unravel the molecular mechanism whereby the Myo1b-dependent recruitment of actin to endosomes regulates endosomal morphology and endocytic trafficking.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'INTegration of EmotioNs During Decision-Making' (INTENDeD), addressing dissemination and exploitation.

assistant: **Impact**

Emotions are often contrasted with reason. However, integration of emotional information into the decision-making processes can advantageously bias adaptive behavior selection. Miss-regulation of emotional information integration in cortical processing is a hallmark of psychiatric disorders with affect dysregulation. Our knowledge of the etiology and pathophysiology of these disorders is strongly limited by our understanding of the non-pathological neuronal circuits underlying emotional processing and makes targeted intervention methods scarce. I here propose to investigate information transfer between two hub regions involved in valence assignment to environmental stimuli and integration of this information in decision making. Gaining insight into the long-range connectivity between the basolateral complex of the amygdala (BLA) and the medial prefrontal cortex (mPFC) and the integration of BLA information in the local mPFC circuit in mice will significantly advance our understanding of emotional processing during decision making. I hypothesize that the BLA can influence the mPFC in a valence-specific manner. To address how valence representation encoded in the BLA biases action selection in a decision-making task, I am going to train mice in a cost-benefit task and modulate valence-specific BLA projections to the mPFC. Furthermore, using in vivo two-photon calcium imaging and state-of-the art long range connectivity mapping methods, I will characterize if the BLA transmits differential valence information to specific mPFC subregions. Finally, I will investigate how valence-specific BLA information is integrated in the local mPFC circuit, using optogenetics based functional connectivity mapping methods in the acute brain slice preparation. This research will allow for t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Reinventing geo-location for IoT' (Nestwave) under Horizon 2020.

assistant: **Objectives**

Nestwave brings a radically novel solution optimized for IoT geolocation. Based on 11 patents issued and pending, the Nestwave solution improves accuracy in urban canyon environments, increases indoor coverage, and reduces power consumption and costs as compared to existing solutions (2.5x accuracy and 1/10 of the power consumption at 1/3 of the cost of GNSS solutions).The Nestwave solution is a combination of software integrated into a modem or GNSS chip and a cloud-based service used to complete the geolocation calculations, which will be sold to communication modem chipset vendors, GNSS chipset vendors, IoT device suppliers and IoT connectivity suppliers.  Nestwave’s unique, patented signal processing solution provides high accuracy and fast signal acquisition at low signal levels (e.g. behind buildings or indoor with signal strength 1,000 or 10,000 times weaker than in open, outdoor locations) or in the presence of signal reflections in urban environments.Currently, the solution determines timestamps using a hybrid of GPS and 4G signal sources. The project funding will be used in part to create a more comprehensive, higher performance solution based on additional signal sources, including Galileo, BeiDou, 5G and WiFi. The performance of the Nestwave solution is reflected by the strong market interest we have experienced in the past 12 months: we have generated €360k from proof of concept projects related to LoRa geolocation and RTOS development.Assuming an average customer lifetime value of €0.4 per IoT device, our total addressable market is estimated to €1.35Bn in 2022 growing to €2.15Bn in 2025, supporting a revenue forecast of €27M by 2025 and over 300 jobs created (directly and indirectly). Nestwave is led by an executive team combining 80 years of experience i
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Unraveling Ribosome Heterogeneity: Implications for Metastasis and Beyond' in the context of a Horizon Europe call.

assistant: **Objectives**

Protein translation, considered a mere reflection of transcription, emerges as a regulatory hub, shaping proteomes and directing cellular outcomes. This is due to existence of heterogeneous ribosome populations, yielding specialized ribosomes that drive context-dependent translation. While technological advances enable mapping of ribosomal diversity, questions remain as to the prevalence of the phenomenon and the mechanisms by which specialized ribosomes confer specific functions. Studies propose the presence of onco-ribosomes promoting oncogenic translational programs and regulating disease-related functions. Metastasis, the deadly spread of cancer cells to remote organs, is a key process that relies on rewiring of gene expression networks. Accordingly, our work shows that changes in the expression of coding and non-coding genes affect metastatic potential and correlates with patient prognosis. We further show that environmentally-regulated RNA modifications add to ribosomal heterogeneity. Yet, the involvement of specialized ribosomes in metastasis development is mostly unknown. Here, I propose to map ribosomal heterogeneity in metastatic cancers and decipher the 'ribosomal code' empowering specialized ribosomes to promote oncogenic translational programs and drive metastasis. Using cell lines, animal models and patient-derived samples, we will chart metastatic patterns of rRNA and ribosomal proteins (RPs), as well as of snoRNAs and rRNA modifications they guide. Employing in vivo and in vitro assays, we will uncover individual RPs and snoRNAs influencing ribosomal functions and metastasis formation and investigate their mechanisms of action. These genes and their metastatic patterns along with metastasis-specific rRNA compositions may serve as cancer biomarkers and ta
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Twophoton microscopy analysis of phosphoinositide-3 kinase function during primary and secondary T cell activation in vivo' to be submitted under Seventh Framework.

assistant: **Abstract**

The adaptive immune system protects us from nocuous microbes, yet excessive immune reactions can lead to the development of autoimmunity. The initiation of adaptive immune responses takes place in lymphoid organs, including peripheral lymph nodes (PLN). Inside PLN, T cells become activated through the molecular interactions of the T cell receptor with cognate peptide-MHC (pMHC) molecules and costimulatory molecules on dendritic cells (DC). TCR-mediated signals lead to the activation of the phosphoinositol-3-kinase (PI3K) pathway via the PI3Kδ isoform, which is required for full T cell activation in vitro.However, little is known about the role of the PI3Kδ during primary and secondary in vivo T cell activation. Here, we will use twophoton microscopy (2PM) to observe the interactions of antigen-specific control and PI3Kδ-mutant CD4+ T cells and pMHC-loaded DC deep inside PLN of live, anesthetized mice. Comparing the dynamic interaction parameters obtained in 2PM imaging with the flow cytometric analysis of the activation status of control and PI3Kδ-mutant T cells, we will delineate the precise contribution of the PI3Kδ for primary T cell activation in vivo.We will furthermore perform an analysis of the secondary activation of control and PI3Kδ-mutant effector CD4+ T cells in a mouse model of antigen-induced arthritis. Therefore, we will establish an intravital knee-joint 2PM model to follow the dynamic behavior of control and PI3Kδ-mutant effector T cells with antigen-presenting macrophages in the inflamed tissue. We will combine imaging data with disease scoring, flow cytometry and the use of a PI3Kδ-specific pharmacological inhibitor for a comprehensive analysis of PI3Kδ function at sites of inflammation.In summary, we propose to perform an in-depth analysis of the rol
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'RETHINK big: Roadmap for European Technologies in Hardware and Networking for Big Data' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

The objective of the RETHINK big project is to bring together the key European hardware, networking, and system architects with the key producers and consumers of Big Data to identify the industry coordination points that will maximize European competitiveness in the processing and analysis of Big Data over the next 10 years.  Specifically, RETHINK big will deliver a strategic roadmap for how technology advancements in hardware and networking can be exploited for the purpose of data analytics while also taking into consideration advancements in applications, algorithms and systems.<br/>In this project, we will not carry out actual research on Hardware optimizations for Big Data, but rather survey the landscape of opportunities and propose a strategic roadmap from that landscape.  The outcome of the project will be a series of detailed (mentioning specific technologies), realistic (considering our 10-year timeline), verifiable (including target metrics) and coordinated technology development recommendations that would be in the best interest of European Big Data companies to undertake in concert as a matter of competitive advantage.<br/>Practically speaking, the roadmap will be produced as a result of area specific and cross-functional working groups meetings and congresses.  We will initially identify and evaluate the existing competencies across European Big Data application domains and technology providers in Europe and then identify the key European stakeholders, or the established and up-and-coming institutions that possess or are developing the technologies, processes or services that map to these competencies.  From these stakeholder institutions, we will select technology and business experts that will chart the technological advancements, their respective challe
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Innovative multidisciplinary approach to pharma generic production' in the context of a Seventh Framework call.

assistant: **Objectives**

The motivation of PHARMAGEN stems from a recognition that bulk Active Pharmaceutical Ingredients (API) manufacturers need to employ new synthesis routes and better manufacturing techniques for the manufacture  of generics drugs. It is also recognised that a key enabling technology for achieving this goal entails greater use of continuous processes. A successful outcome will deliver processes which are more energy efficient, have higher yields, better controllability and quality, greater flexibility of output and faster development time. Most importantly, development of effective continuous processing methods will give European manufacturers a significant competitive advantage over Asian producers who have been  progressively eroding the European know how and market share leadership in the API market. The PHARMAGEN project aims to improve the competitiveness of the participants by developing continuous production routes for three specific APIs selected by the SMEs.  Batch pharmaceutical processes and continuous petrochemicals  source their raw materials from the same basic chemical pool. Despite this, yield indexes (= wt reactants/wt final product, representing the effectiveness of the processes) for high throughput plants are typically in the region of 1,2 - 1,5, compared to more 10, for pharmaceutical batch processes. Thus, traditional pharmaceutical processes are characterised by  waste levels of 90% plus.  The PHARMAGEN project will allow  participating  SMEs to develop new and better chemical synthesis methods based on continuous processing.  Further innovative modelling and process control will be developed. This will give European pharmaceuctical manufacturers a competitive advantage.   Continuous processes can allow very high control level respect discontinuous o
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Membrane machineries for protein and solute transport through the bacteria cell envelope' goes beyond current state-of-the-art under Fifth Framework.

assistant: **Innovation**

This programme brings together laboratories with firmly established reputations in the biochemical and biophysical (partners 4 and 5), genetic and molecular biological (partners 1, 3 and 8) and structural (partners2, 6 and 7) analysis of bacterial membrane protein complexes. Because aspects of each project are amenable to analysis by several different approaches, we plan very frequent, long-term exchanges of junior scientists for training in other partner laboratories to increase familiarility with the different methodologies, to adapt them to the specific needs of individual projects and to resolve technical problems. The members of the network undertake to provide the highest possible standard of broad-based technical training and personal supervision of visiting scientists and to provide an excellent, multidisciplinary working environment conducive to efficient and rapid progress.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Nwc2002' under Fifth Framework.

assistant: **Concept**

The Neurochemistry Winter Conferences are designed to provide high-standard scientific conferences combined with advanced training for young researchers, with a specific focus each year. In the field of drug abuse research, the last decade has seen the highly profitable cooperation between gene targeting, neuroimaging, and more traditional neurochemical / neuroanatomical /electrophysiological approaches on one side and behavioural experiments on the other side, leading to important advances in the understanding how different neuronal systems contribute to the reinforcing effect and the abuse liability of addictive drugs. However, a number of issues are still unresolved. The workshop proposes to bring together experts from all cutting-edge experimental approaches to drug abuse and dependence to discuss the benefits and shortcomings of each experimental paradigm in the expectancy of cross-fertilization. Most drug abuse research has been conducted in the U.S.A., resulting in a focus on drugs that are more prevalent there. Consequently, a number of drugs of abuse that constitute more of a European problem", such as MDMA ("Ecstasy"), have been under-researched. The European-added value of the topic and for the participants consists on it focus on European research efforts and drugs of abuse that are prevalent in Europe (without neglecting the contribution of the scientific community in the U.S.A.), thus giving credence to and foster the future development of a stronger European drug abuse research task force.The disciplines represented by the invited keynote lectures are gene targeting, neuroimaging, neuranatomy, neurochemistry, electrophysiology, behavioural research in animals and humans, and clinical research. The programme will be supplemented by contributions of young s
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Virus-Inspired Colorectal Cancer Treatment through an Orally Administered Vaccine' demonstrating excellence, impact and implementation quality for a Horizon Europe call.

assistant: **Abstract**

Colorectal cancer (CRC), which includes cancers of the colon and rectum, represents a significant global health challenge as the third most commonly diagnosed and second most fatal cancer worldwide. It also places a substantial burden on European patients and healthcare systems. While standard treatments are available, they face significant limitations, especially in managing advanced CRC, often leading to poor patient outcomes and severely compromised quality of life. Emerging immunotherapies like immune checkpoint inhibitors (ICIs) and adoptive cell therapies show promise but face major obstacles, including high costs, side effects, lack of tumor targeting, and, most critically, they fail to treat ""cold"" CRC tumors with low or absent pre-existing anti-cancer immunity. Prof. Shi and his team have developed an innovative oral cancer vaccine to transform these “cold” CRC tumors into “hot” tumors by provoking potent cellular and humoral anti-cancer immunity. The vaccine will achieve this through its acid-stable nanovesicle structure to induce potent and intestine-localized anti-cancer immunity with reduced systemic side effects and improved patient compliance. This project aims to explore the technical and commercial potential of the vaccine platform.""
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'Enjoy yourself with researching'.

assistant: **Objectives**

A huge lot of humour, commentary by popular scientists, crazy experiments and answers to questions - can a man fly, can a human voice break glass, how to get rid of rubbish and what does the size of the moon depend on? Apart from that, there will be the public's  favourite workshops, during which you can write a computer programme, create a multimedia game, trace a flying object or bake a pizza - and everything hand in hand with the researchers! There will also be many competitions in which the whole families can participate. The eyes of the visitors will see mysterious places, usually closed and unavailable. The authorities from the world of science will come down from their chairs for this one Night in a year, and, in extraordinary atmosphere, they will tell stories about their journeys, politics, latest scientific discoveries... All of this and some more will happen on this very night, the Researchers' Night! The stars of the evening will be our magnificent scientists. The actors, recipients, co-creators - all the inhabitants of our region, waiting impatiently for September! The fanclub of the Night is increasing in size. It is all due to the programme which gets more attractive every year and is based on the participants' suggestions. Having fun with the whole united Europe is a windfall for the inhabitants of Gliwice, and the idea of the European Commission ' To teach by playing ' is welcome with more and more enthusiasm. A rich and varied programme of the event is a guarantee of a great fun with the researchers who can 'infect' - especially young people - with their passion of discovery, but simultaneously they will reveal that love to science does not exclude dreams and hobbies beyond it. We can assure that the Night will give unforgettable impressions and will a