RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'High Temperature Sensors' under Seventh Framework.

assistant: **Concept**

High-temperature piezoelectric sensing technology is of major importance for the chemical and material processing, automotive, aerospace, and power generating industries. Aurivillius phase ceramics are of interest for these applications because of their high Curie points (Tc up to ~950C). Modified bismuth titanate compositions can be used for sensor applications up to 500C. If a higher operating temperature is required there are currently no suitable polycrystalline ceramics available. The objective of this project is to systematically investigate the effect of doping on the properties (ferroelectric, piezoelectric, dielectric, Curie point, electrical conductivity, thermal depoling and ageing) of candidate Aurivillius phase ceramics. Having identified promising optimised compositions, we will then determine a processing route using Spark Plasma Sintering (SPS) to produce highly textured microstructures with enhanced piezoelectric coefficients.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Neurodegeneration in Alzheimer's disease - mechanisms, consequences and therapy' under Sixth Framework.

assistant: **Concept**

The aim of this project is to provide structured research training for early stage researchers in the field of Neurosciences, with special emphasis on Alzheimer's disease (AD). Due to the demographical development of societies in industrialized countries, the number of AD patients will increase dramatically within the next years.Thus, the demand for well-trained scientists in pharmaceutical companies and academic research units focussing on AD is expected to rise. To be able to meet the future requirements, a consortium of AD scientists from 9 universities have gathered to implement a Marie Curie Early Stage Training, named NEURAD (acronym for neuron" and "AD") together with one pharmaceutical company and two SMEs.The common theme of the consortium is to study the early patho-mechanisms of AD, the consequences for the physiology of neuronal networks and for deficits in memory, with the implementation and verification of novel therapeutic approaches for AD being one of its main targets.The fellows will be trained in four complementary modules.Mandatory:- Interdisciplinary experimental training in another university based laboratories within the consortium for at least 6 months.- Multidisciplinary courses in AD research and management skills during an annual two-week summer school as complementary training.- An intersectional 3 months research training in a laboratory of the pharmaceutical partners.Optional:- One-month clinical internship in a clinical memory unit. These actions will manifest the mobility of the fellows in a European perspective, and implement the truly interdisciplinary, interscectional and multidisciplinary character of the NEURAD initiative."
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Evolutionary discovery of novel drugs by orchestration of polymer-supported combinatorial bio-/chemistry' demonstrating excellence, impact and implementation quality for a Fifth Framework call.

assistant: **Abstract**

The overall aim of the project is the synthesis of novel chemo- and biocatalytic combinatorial libraries which will be screened for biological activities (e.g. as antibacterial or anticancer agents). This includes development of novel methodologies for reactions on support, either on soluble high molecular weight polymers or on solid supports together with a new class of enzymatically cleavable linkers. Enzyme cleavable linkers allow release of compounds directly into assay mixtures, because release is in aqueous medium. The application of enzymes and whole cells for a biocatalytic combinatorial approach offers the opportunity to combine functionalities in macrocyclic target structures, which are difficult to obtain by traditional chemical methods.During the first project period the following results hae been achieved:- Recombinant penicillin G acylase (PGA) Recombinant PGA originating from two different origins can be produced using efficent expression systems. These two enzymes differ from the commercially available PGA with respect to stability, activity and substrate specificity. Furthermore the clones now offer the possibility for directed evolution of the enzyme to improve its cleavage of the linker;- Insoluble carriers with improved properties Insoluble supports based either on polystyrene or polyethylenglycol have been modified in order to improve its swelling behavior in various solvents and therefore give better access to the linker for the enzymes;- Penicillin G acylase cleavable linkers Based on modelling of the linker geometry in the active site of the enzymes derivatives of the linker have been developed which are cleaved by the enzyme with better results. For a novel type of linker also other hydrolytic enzymes can be used for cleavage;- Membrane process
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon 2020 proposal for the project 'Tiny Functional Au Nanorods: Novel NIR-Photothermal Nanoprobes for Single-Molecule Tracking at Confined Cellular Environment'.

assistant: **Objectives**

Photothermal Imaging (PhI) is an absorption detection technique used for the detection of single gold nanoparticles (NPs), which can be a valuable tool to tackle complex cellular activities. In order to study the dynamics of individual proteins in confined regions such as in neural synapses or in cell adhesion sites, small nanoprobes are needed. Gold NPs (~ 5 nm) can be used, however their surface plasmon resonance (SPR) is centered at 530 nm, a spectral domain where absorption of cellular organelles results in a background signal, which limits the sensitivity of PhI. A brilliant way to find the needle in the haystack is the development of a novel nanoprobe having NIR-absorption, smaller size and high specificity. In “TiFuN”, we will achieve this goal by developing tiny (length/diameter <10 nm/5 nm) gold nanorods (T-rods), an elongated gold NP with tunable SPR in the NIR-window. Different aspect ratio T-rods harvested using Density Gradient Ultrahigh (DGU) centrifugation protocol will be highly promising probes for multicolor labeling and detection of proteins. A dedicated PhI setup based on NIR-laser sources will also be built. Subsequently, the bioconjuagted T-rods and NIR-PhI will be used to study the dynamics of neurotransmitter receptors in synapse and integrin proteins in cell adhesion sites owing to their small core volume, NIR-absorption, exceptional photostability, and non-toxicity. Bioconjugated T-rods will also display the unique property of being easily detected with light and electron microscopy at the single- molecule level, and therefore will be a valuable tool for light/electron correlative microscopy. This highly interdisciplinary project (optics, chemistry and biology) will utilize the fellow’s expertise in nanochemistry in combination with world-leadi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Hydropower infrastructure in Andean headwaters: Combining Observations and Remote sensing to analyse Environmental impacts' to be submitted under Horizon Europe.

assistant: **Abstract**

Hydroelectric power is a popular alternative to burning fossil fuels, especially within the Amazon river basin. However, no single hydropower installation is without any environmental impact. By damming a river, significant alterations do occur in the hydrological connectivity, which reverberate in the river’s morphological state and physicochemical conditions. Moreover, consequent changes in the structure and functioning of the aquatic community have a tendency to negatively affect various ecosystem services, thereby putting local societies at risk and undermining the goal of sustainable development. Various qualitative global assessments have certainly helped illustrating opportunities and threats, but have failed to be compatible with local management needs and ecological perspectives. Therefore, the aim of this research is to monitor, analyse, evaluate and illustrate the impacts of reservoir-based hydropower infrastructure on the morphology, hydrology and quality of Andean headwaters within the Amazon river basin by combining (i) remote sensing, (ii) process-based hydrological modelling and (iii) water quality assessment based on field campaigns with a high temporal resolution. Additional focus is directed at the interaction with climate change, including the contribution through emissions of greenhouse gases (field measurements) and the challenges under future climate conditions (modelling). To do so, the Paute river basin (Ecuador) is selected as study area to (i) overcome the local data scarcity, (ii) develop a protocol for evaluating similar systems and (iii) preserve the uniqueness of these headwater ecosystems. Project activities and obtained results will be publicly accessible and continuously disseminated online and in-person. As such, this research will inc
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Fifth Framework proposal on 'Sensor array for fast explosion proof gas monitoring (SAFEGAS)'.

assistant: **Concept**

Semiconducting oxide sensors (e.g. Tagushi-sensors) used for olfactory imaging at present are inherently too slow to provide instantaneous response required for real-time process monitoring and control in the industry. Besides they are prone to humidity effects limiting reproducibility. This is a compelling reason to look for alternatives. The targeted research for the development of an integrated microsensor array and adequate signal processing aims at the olfactory detection and compositional analysis of combustible gas mixtures even without the risk of explosion. Calorimetric catalytic sensors by principle do not suffer from humidity effects, which is the motivation for the selection of this principle for array construction dedicated to olfactory imaging in the proposal. The sensor structure will be utilised for monitoring and control of industrial processes, in environmental applications but also for maintenance of the safety at the workplace and at home. In this sense the socio-economic impact is rather large regarding the quality of life, the safety and working conditions. The consortium is an interdisciplinary team utilising proprietary know-how obtained at different European institutions. The industrial partner relays the market-demands even at this early stage of development in line with EU standards and is the potential end user of the results.The primary objective of this consortium was the development of a new generation, small size, low power consumption gas transmitter for monitoring the concentration of combustible gases. As a result of the collective Safegas project work, a sophisticated porous silicon micromachined chip, several catalytic materials and other gas sensing hardware and software able to distinguish between 4 hydrocarbons (CH4, C2H6, C3H8, a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Spiking network mechanisms underlying short term memory' (HIRESMEMMANIP).

assistant: **Innovation**

Short term memory (STM) is impaired at old age and a host of neuropsychiatric disorders, and has been the focus of a multitude of psychological and theoretical studies. However, the underlying neuronal circuit mechanisms remain elusive, mainly due to the lack of experimental tools: we suggest that rapid manipulations at the neuronal level are required for deciphering underlying mechanisms. We have developed an approach combining large-scale extracellular recordings and high density multi-site/multi-color optical stimulation (“diode-probes”), which enables high resolution closed-loop manipulation of multiple circuit elements in intact, free, behaving rodents. After training mice and rats to perform bridging-free STM-tasks, we will evaluate local circuit mechanisms in hippocampus and prefrontal cortex. Two broad classes of manipulations will be used: First, necessary components and timescales needed for STM maintenance will be established by focal real-time silencing of specific cell types and real-time jittering of spiking in those cells. Second, sufficient components (neuronal codes) will be determined by a circuit-training phase, in which novel associations between synthetic brain patterns and behaviorally-relevant short-term memory traces will be established. The first class is equivalent to erasing memories and the second to their writing. Together, these manipulations are expected to reveal global and local circuit mechanisms that facilitate STM maintenance in intact animals


**Keywords:** Optogenetics, precise timing, memory, oscillations, mice, rats, hippocampus, prefrontal cortex, extracellular
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Solid state physics methods applied to surface chemistry and catalysis problems' goes beyond current state-of-the-art under Fourth Framework.

assistant: **Innovation**

Automobile exhaust emmissions have been among the primary sources of air pollution in most major cities for the few decades. In an effort to decrease air pollutants from car exhausts, substantial research has been done on catalytic reduction of NO by CO. Current state-of-the-art solid state physics methods, such as Density Functional Theory (DFT) calculations allow for the accurate and detailed characterization of heats of reactions and activation energy barriers. The main objective of the proposed project is to implement DFT slab-type calculations to explore some of the critical mechanistic details of the NO+CO reaction over the most commonly used Pt-Rh catalyst. ] the adsorption and dissociation of NO on (1) pure Pt, (2) pure Rh single crystal surfaces and (3) different compositions of a bimetallic Pt-Rh surface alloy will be investigated. Similar work is proposed for CO. permitting, the above elementary reaction steps over other interesting metals (Ir, Pd) and alloys (Au-Rh, Au-Pt) be investigated. Such alloys are expected to allow fine-tuning of catalyst activity and selectivity. The ultimate goal to identify the mechanistic trends for the NO+CO reaction over different surfaces and propose a good candidate replacing the current catalyst, suffering from the fast decline of Rh natural reserves. Training content (objective, benefit and expected impact): The completion of the research project proposed would guarantee the multifaceted training of the applicant the area of DFT methods as applied to the prediction of solids' properties and chemical reactivity of surfaces. These methods have already been proven to treat the central problem of catalysis, namely chemisorption, with remarkable accuracy. The applicant will gain an active knowledge of DFT methods during the cour
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Electrodynamic braking of large wind turbines' demonstrating excellence, impact and implementation quality for a Fourth Framework call.

assistant: **Abstract**

State of the art Wind turbine currently use a combination of mechanical and aerodynamic braking devices. The mechanical systems tend to be expensive, are subject to mechanical wear and cause impulsive drive train loads which result in additional costs either in design against those loads or in failures. An Electrodynamic Brake (EDB) offers an attractive alternative which has been used in other industries. Garrad Hassan (GH) are leading a preliminary investigation of EDB's on behalf of the UK Department of Trade and Industry. This study has explored the EDB state-of-the-art, the view of the wind turbine industry and the design issues. It has emerged that there is interest in this technology and that a demonstration at large commercial scale is required to allow this approach to gain real credibility. The advantages of EDB's increase with machine size. The application of EDB technology at the proposed size is highly innovative. Objectives - To reduce the impact loads during braking transients by 50% - To reduce the cost of a typical commercial 600 kW wind turbine by 1% - To increase reliability of machine safety systems particularly in developing countries where grid failures are frequent and hence mechanical brake wear is high. These prime objectives will be realised through the achievement of the secondary objectives : - To design, install and operate an electrodynamic braking system for a 600 kw commercial wind turbine. - To evaluate its technical and economic performance compared to a conventional mechanical system. SCOPE The conclusions will be applicable to all wind turbines with a grid connected induction generator-presently the majority of commercial machines. The potential advantages offered by electrodynamic braking are listed below : - Low cost and simplicity.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Monitoring calcium concentration in the endoplasmic reticulum using recombinant aequorin specifically targeted' (1994-1998).

assistant: **Concept**

The project proposed constitutes an extension of that carried out during my stay in the University of Padova. I will first construct a set up to measure aequorin luminescence, similar to those actually running in Padova. Then, I will use the HeLa clones I have obtained in Padova, which stably express the DNA construct designed to target aequorin (both native and mutated with low Ca2+ affinity) to the endoplasmic reticulum (ER). These cells will be used to continue the study of ER-Ca2+-heterogeneity currently being carried out in Padova. At the same time, I plan to produce a series of point mutations in the Ca2+ -binding sites of aequorin in order to reduce its Ca2+-affinity up to near the mM range. In the mean time, Sr2+ will be used as a low-affinity Ca2+-surrogate. Sr2+ has been shown to behave similarly to Ca2+ in terms of uptake, accumulation and release from the ER.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'A biophysical model for ESCRT-III mediated membrane scission' (2014-2020).

assistant: **Concept**

My project focuses on the characterization of the mechanism of action of ESCRT-III complex. ESCRTs are evolutionary conserved proteins which mediate membrane remodeling and scission in a variety of important cellular processes, and are implicated in the pathogenesis of many diseases, including virus release and nuclear membrane repair during cancer cell migration. Despite intense research, many outstanding questions still remain unanswered, and all model proposed remain highly speculative. Based on our preliminary data, we propose a novel, unifying model for ESCRT-III function. The aim of the project is to provide solid data to support this model by an in vitro reconstitution approach, which would constitute a major contribution in the field. The concrete aims of my project are:- Characterization of the minimal ESCRT-III fission machinery - Characterization of the molecular mechanism leading to membrane fission by ESCRT-III complex- Characterization of the membrane requirements to obtain fission by ESCRT-III, in particular membrane curvature


**Keywords:** ESCRT-III,  membrane scission,  cytokinesis,  MVB,  HIV budding, membrane repair,  CHMP,  negative membrane curvature