RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Liquid Foam Therapy (LIFT) for Acute Respiratory Distress Syndrome (ARDS)' (LIFT) under Horizon 2020.

assistant: **Objectives**

Acute Respiratory Distress Syndrome (ARDS) is an inflammatory lung condition caused e.g. by sepsis, pneumonia and head or chest injury, affecting annually 133,000 people in Europe and 255,000 in the US. ARDS is characterized by the depletion of the lungs’ inner liquid coating (pulmonary surfactant), which reduces surface tension forces and allows the lungs to expand. Patients span across all age groups and lay anesthetized in the intensive care unit with mortality rates at 40%. Although surfactant replacement therapy (SRT) is a life-saving procedure in newborn neonates, current administration methods to treat adults and even children remain inadequate. Endotracheal surfactant liquid instillations used in babies fail in larger lungs: liquid drains into pools, drowning these lung regions while leaving others untreated. Meanwhile, inhalation aerosols can only deliver small doses (<1ml/hr for nebulizers), far from the required ~100ml of surfactant. To overcome such hurdles, we advocate using liquid foam as a surfactant carrier to the lungs, i.e. LIquid Foam Therapy (LIFT). Foam is weakly affected by gravity, distributes homogeneously within lungs and in abundance. LIFT brings a paradigm shift in SRT, and more broadly, in the field of therapeutic pulmonary delivery. Our team bridges expertise in clinical ARDS treatment in neonates, pulmonary medicine, biomedical engineering and business. Together, we have demonstrated the feasibility of our patent pending technology, both in vitro in 3D printed and microfabricated airway models, and ex vivo in excised pig lungs using fluoroscopy. In this PoC, we will optimize the foam formulation, design and construct a delivery device, and run pre-clinical in vivo animal experiments. LIFT has the potential to extend far beyond ARDS treatmen
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Seventh Framework proposal on 'Physiological and Behavioural Photoprotective Processes against Oxidative Stress in Marine Photosynthetic Symbioses'.

assistant: **Innovation**

Marine sacoglossa sea slugs, mainly of the genus Elysia, have developed a unique functional nutritional mode in which they gain the capacity for phototrophic-mediated carbon acquisition by sequestering macroalgae plastids into tubule cells of their digestive diverticula (kleptoplasty). as occuring in algae and plants, exposure to excessive light is expected to be a major cause of stress to the photosynthetic apparatus of photosynthetic symbioses. When the absorbed light energy exceeds the capacity of photochemical pathways, reactive oxidative species accumulate in the cell and cause damages to the photosynthetic apparatus (photoinhibition), mainly through the degradation of protein D1 in photosystem II.  To cope with high ambient light levels, algae and plants have developed a range of physiological photoprotective processes, the most important being the xanthophyll cycle, the antioxidant enzyme system and the de novo synthesis of D1 protein. In the case of marine photosynthetic symbioses typically inhabiting surface waters or intertidal habitats, the efficient functioning of photoprotective mechanisms may be of crucial importance for their survival as photoinhibition reduces the amount of photosynthetically fixed carbon made available to the animal host and damage the photosynthetic apparatus in the chloroplast. Anatomical and behavioural traits can also enhance the performance and functional longevity of chloroplasts in different light regimes. This adaptation would be functionally equivalent to chloroplast avoidance movements and leaf fold described in plants, and the migratory behaviour of motile microalgae, shown to provide effective photoprotection against photoinhibition. This work will provide new insights into the photophysiology and photobehaviour of a marine
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'A new paradigm for centromere biology: Evolution and mechanism of CenH3-independent chromosome segregation in holocentric insects' (CENEVO), addressing dissemination and exploitation.

assistant: **Impact**

Faithful chromosome segregation in all eukaryotes relies on centromeres, the chromosomal sites that recruit kinetochore proteins and mediate spindle attachment during cell division. Fundamental to centromere function is a histone H3 variant, CenH3, that initiates kinetochore assembly on centromeric DNA. CenH3 is conserved throughout most eukaryotes; its deletion is lethal in all organisms tested. These findings established the paradigm that CenH3 is an absolute requirement for centromere function. My recent findings undermined this paradigm of CenH3 essentiality. I showed that CenH3 was lost independently in four lineages of insects. These losses are concomitant with dramatic changes in their centromeric architecture, in which each lineage independently transitioned from monocentromeres (where microtubules attach to a single chromosomal region) to holocentromeres (where microtubules attach along the entire length of the chromosome). Here, I aim to characterize this unique CenH3-deficient chromosome segregation pathway. Using proteomic and genomic approaches in lepidopteran cell lines, I will determine the mechanism of CenH3-independent kinetochore assembly that led to the establishment of their holocentric architecture. Using comparative genomic approaches, I will determine whether this kinetochore assembly pathway has recurrently evolved over the course of 400 million years of evolution and its impact on the chromosome segregation machinery.My discovery of CenH3 loss in holocentric insects establishes a new class of centromeres. My research will reveal how CenH3 that is essential in most other eukaryotes, could have become dispensable in holocentric insects. Since the evolution of this CenH3-independent chromosome segregation pathway is associated with the independent
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Fifth Framework proposal for the project 'Water flow and contaminant transport in fractured aquitards'.

assistant: **Objectives**

Aquitards (low-permeability rock formations) bear attractive features for waste isolation, as they are expected to act as natural hydrological barriers, segregating potential contamination at land surface from the underlying aquifers. However, the heterogeneous structure of these formations, which contain highly transmissive fractures, poses serious questions regarding the efficacy of aquitards as natural barriers. This meeting focuses on the critical, cutting-edge research theme of how water and contaminants migrate through fractured aquitards. The meeting covers a wide range of innovative research problems, including advanced geophysical and hydro geological characterization methods, theoretical models of flow, transport and dispersion, geochemical interactions among fluids, contaminants and the host rock, and microbiological aspects. Integration of these methods and approaches is essential to development of realistic, quantitative models for flow and transport. Such models are critical to sustainable management and protection of groundwater resources, and to evaluation of a variety of waste isolation scenarios.ftp://ftp.cordis.lu/pub/improving/docs/HPCF-2000-00331-1.pdf
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Testebed for a Worldwide Agent Network' (1998-2002).

assistant: **Innovation**

Agentcities NET will create an open dynamic service environment based on Agent technology by supporting users wishing to deploy agent platforms and services that connect to an extensible Internet based testbed. The project will also enable technology transfer by bringing together key players from related industries (potential users of the technology) and those developing testbed infrastructure and services - potential technology providers but also early take up users - with the testbed as a focal point for collaborative work. The trial will open to encourage Europe-wide participation and has over 40 pre-registered contributors. Concretely, the trial will involve a set of coordinated actions", designed to support these activities: information days, a deployment support program, a competition, student exchange support and focused working groups.Objectives:The primary objectives of this project are:I) creation of a Europe-wide, extensible, open testbed network of agent platforms, to enable users to connect up their own systems at will to grow the network and begin to explore the potential of the technology,2) support technology transfer by facilitating discussion, input and feedback on all aspects (technical, scientific, legal, business) of the evolving open network.The project expects to produce a valuable public resource for agent research and pre-competitive commercial exploitation, bring together key players from related industries (tool vendors, hosting services, portals, e-commerce solution providers, content providers).Work description:The project will work closely with an associated project Agentcities.RTD (Proposal number IST-2000-28385) which will develop a backbone of 14 Agent platforms worldwide. This project will build on the Agentcities.RTD effort to enable o
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Structural elucidation by x-ray crystallography of p62, a nuclear pore subcomplex involved in signal mediated nucleocytoplasmic transport' in the context of a Sixth Framework call.

assistant: **Objectives**

Nuclear pore complexes (NPCs) are large multi-protein structures (~125 MDa) spanning the double membrane of the nucleus and acting as the only gateway of nucleocytoplasmic transport. The three dimensional (3D) architecture of the NPC, its role in trafficking of proteins and RNA, and its molecular composition (nucleoporins 'Nups') have been extensively studied.Nonetheless, to understand how the NPC mediates nucleocytoplasmic exchange requires the independent characterization of its molecular components at atomic resolution and a detailed description of physical interactions between nucleoporins in the NPC assembly. Crystal structures of three nucleoporin domains have been resolved (Nup98, hNup133, Nup159), although no atomic model for any sub-complex of N ups is yet available.This proposal aims to elucidate the structure of the nuclear pore subcomplex p62, a biomedical agent essential to signal-mediated nucleocytoplasmic transport, using 'bi-cistronic multiple co-expression' for in vitro complex reconstitution and X-ray crystallography. This accomplishment will contribute to the construction of parts of the NPC at atomic detail essential to understand nucleocytoplasmic trafficking and to establish basic knowledge into human diseases caused by disruption of transport pathways (objective of the LifeSciHealth Priority Programme of Framework 6).The applicant, of Spanish nationality, will accomplish this task under the supervision of Dr. Olga Mayans at the renowned Swiss institute Biozentrum (in compliance with the section 2.3.2.1 of the WP HRM). The multidisciplinary and advanced training received by the applicant will complement and expand her technical experience and research management capabilities to initiate an independent career in Molecular Structural Biology.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Dissecting the biophysical mechanisms of deep brain stimulation using voltage fluorescence microscopy' (OptoDBS) under Horizon Europe.

assistant: **Objectives**

Deep brain stimulation (DBS) is a promising neuromodulation technique for the treatment of neurological and psychiatric diseases. DBS electrically stimulates brain tissue via implanted electrodes. However, the biophysical and therapeutic mechanisms underlying DBS remain largely unknown. While long-standing theories suggest that DBS suppresses neurons, subsequent studies have shown that DBS activates neurons via direct axonal excitation modulating membrane dynamics, information transfer, and network synchronization. Unfortunately, this conundrum has been difficult to resolve experimentally because of (1) the electrical interference occurring in electrode-based recordings and (2) the challenge of measuring cellular-level membrane voltage dynamics in the awake brain. In this proposal, I will overcome for the first time both key challenges by using a novel in-vivo voltage fluorescence optical technique that permits the direct recording of the membrane voltage from identified neurons in awake animals, free of electrical interference. I will combine voltage fluorescence microscopy and electrical stimulation in a mouse model of epilepsy to optically dissect the cellular mechanisms underlying therapeutic DBS for reducing seizures in the limbic system. This innovative and comprehensive approach will provide novel insights into the cellular and circuit processes occurring during DBS in the awake epileptic brain. This research program will provide an unprecedented opportunity to build a mechanistic framework of DBS and stimulation parameter selection with the ultimate goal of identifying more effective and targeted DBS protocols for human patients.


**Keywords:** Deep brain stimulation, Voltage imaging, epilepsy
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Opening Up Science' under Horizon 2020.

assistant: **Concept**

While 2020 can be defined using the word ""Lockdown"", we hope to define 2021 with the words ""Opening Up"". If the world is about to return to normality, the key contributor to this achievement is Research. Thus, the 2021 European Researchers? Night aims to celebrate science, as the hero that made it possible for the world to open up.Designed around the concept of ""Opening Up Science"", our event will highlight the impact that science and researchers have had in the process of opening up the world again. Never before in the history of humanity has the impact of science on our lives been clearer and faster. The concept will be extended to underline the key role that research will play in a post-Covid green recovery, a process that will allow societies to move forward to a cleaner, more sustainable, carbon-neutral and resilient future. Public trust in science comes with a higher responsibility for researchers in society: Opening Up Science will also carry a key message to the research community that needs to open up to the public. Using our event as a best practice example, we will highlight the importance of public engagement and science education as vital elements in making science truly more open in the society.The event will be organised by a national consortium, based in large Romanian university cities (Iasi, Timisoara, Craiova, Constanta and Sibiu), in collaboration with their traditional partners and local authorities, as well as joining goals with local science festivals or science associations. Flexibility will be key principle in organizing of the 2021 - whether returning to face-to-face interaction or remaining in an online format, focused on minds-on experiments, the event will capitalise on the newly gained experience of 2020, that allowed for better reach
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Novel Algorithmic Techniques through the Lens of Combinatorics' demonstrating excellence, impact and implementation quality for a Horizon 2020 call.

assistant: **Abstract**

Real-world optimization problems pose major challenges to algorithmic research. For instance, (i) many important  problems are believed to be intractable (i.e. NP-hard) and (ii) with the growth of data size, modern applications often require a decision making under {\em incomplete and  dynamically changing input data}. After several decades of research, central  problems in these domains have remained  poorly understood (e.g. Is there an asymptotically most efficient binary search trees?)  Existing algorithmic techniques either  reach their limitation or are inherently tailored to   special cases.   This project attempts to untangle this gap in the state of the art and seeks new interplay across  multiple areas of algorithms, such as approximation algorithms, online algorithms, fixed-parameter tractable (FPT) algorithms,  exponential time algorithms, and data structures. We propose new directions from the {\em structural perspectives} that connect the aforementioned algorithmic problems to basic questions in combinatorics.Our approaches fall into one of the three broad schemes: (i) new structural theory, (ii) intermediate problems, and (iii) transfer of techniques. These directions  partially build on the PI's successes in resolving more than ten classical  problems in this context. Resolving the proposed problems will likely revolutionize our understanding about algorithms and data structures and potentially unify techniques in  multiple algorithmic regimes. Any progress is, in fact, already a significant contribution to the algorithms community. We suggest concrete intermediate goals that are of independent interest and have lower risks, so they are suitable for Ph.D students.


**Keywords:** Approximation Algorithms, Hardness of Approximation, Data Structures, Online
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Saving vision: expanding use of lentiviral gene therapy to fight blindness' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

Sight is the most treasured of our senses, followed by hearing and balance. Moderate to severe vision loss affects more than 216 million people worldwide, with an estimated 36 million people suffering from blindness. Monogenic diseases caused by mutations in genes such as MYO7A are responsible for vision loss in over two million people. As current treatment options for genetic vision loss are limited or non-existent, there is a high clinical need for innovative approaches, such as gene therapy. Usher syndrome type 1B (USH1B) is caused by MYO7A mutations and patients suffer from profound deafness accompanied with loss of balance and vision. In my ERC-funded consolidator grant iHEAR and Proof-of-Concept grant MY(O)SENSES, we showed the first ground-breaking data for efficient gene transfer with lentiviral vectors into inner ear cell types and correction of hearing and balance defects caused by mutated MYO7A. The EuroVision PoC proposal aims to advance these valuable research results towards clinical application by completing pre-clinical development, securing IP rights and commercializing a formulated medicinal product to launch a first-in-human clinical LV gene therapy trial to treat USH1B patients. The EuroVision product will be the first and only single-vector treatment that addresses vision loss due to MYO7A mutations in USH1B patients, which has great potential to significantly improve quality of life for these patients as well as to benefit society through relief of costs associated with healthcare and lost productivity. Moreover, this ground-breaking therapy will lay the basis to address other monogenic eye diseases, in which large genes need to be transferred and for which there is an urgent medical need.