RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Generating a targeted, brain-permeable and stable polymeric nanoparticle for systemic gene delivery to glioblastoma' (TargetGBM).

assistant: **Concept**

Glioblastoma multiforme (GBM) is the most prevalent deadly brain tumour and is currently incurable. Patients die from recurrent tumours that grow from cells surviving the first round of treatment. This is mainly due to the narrow therapeutic window of current drugs and to the low permeability of these drugs across the blood-brain barrier (BBB). Targeted non-viral delivery systems have shown great promise to decrease side effects of therapeutics and to enable the transport across biological barriers. The main objective in this proposal is to generate a dually targeted nanoparticle loaded with mRNA encoding a conditionally toxic protein to treat brain cancer. The nanoparticle will be modified with two ligands: an antibody for targeting tumour cells and a peptide that enables transport across the BBB. In addition, I will develop an acid-labile coating that enhances the stability of the polyplex in blood and is released upon internalization. This coating will present the ligands in a defined orientation using bioorthogonal chemistry, thereby maximising their targeting efficiency. The chemical handles will be introduced using genetic code expansion in the antibody and solid-phase synthesis in the peptide.  A variety of formulations will be screened in vitro to evaluate their stability and transfection efficiency, and BBB permeability will be assessed in a human cell-based BBB model. Subsequently, the efficacy of the most promising candidates will be tested in a GBM mouse model. The combination of a polyplex having high transfection efficiency with a sheddable coating and oriented targeting ligands is designed to generate the first efficient non-viral gene delivery system for the systemic treatment of GBM, reaching all tumour cells to provide a more efficient treatment and po
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'EU Training Network in understanding the molecular regulation and the role of endo-lysosomal processes in cardio-metabolic diseases' (EndoConnect) under Horizon 2020.

assistant: **Objectives**

The endo-lysosomal system is essential to acquire lipids and nutrients according to the need of the cell and, subsequently, to maintain metabolic homeostasis. The strong connection between the endo-lysosomal system and metabolism is reflected in the observations that defects in this system are correlated with cardio-metabolic disorders, such as cardiovascular disease, type 2 diabetes and non-alcoholic fatty liver disease. The molecular mechanisms that interconnect the endo-lysosomal system with metabolism are, however, poorly understood. This can be largely explained by the fact that (1) endo-lysosomal and metabolic research are carried out independent of each other and (2) the number of model systems to study endo-lysosomal processes at an organismal and clinical level are limited. It is the ambition of EndoConnect to change this by bringing top scientists and industrial partners together to train early stage researchers in interdisciplinary research, with the focus on translating key findings from cellular and animal models to patients with cardio-metabolic diseases. It is our mission to: (1) understand the molecular organization and regulation of endo-lysosomal processes, (2) elucidate how metabolism and endo-lysosomal processes are intertwined, (3) understand how defects in the endo-lysosomal system contribute to the development of cardio-metabolic diseases and, finally, (4) how the endo-lysosomal system contributes to the efficiency of therapeutics. EndoConnect will provide an international training program for early stage researchers to acquire knowledge and state-of-the-art scientific, technical, and transferable skills to connect molecular and cell biology research with physiology and biomedical research towards translation. We expect that EndoConnect will incre
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Resolving the molecular basis underlying convergent evolution of DODA enzymes in the betalain synthesis pathway' demonstrating excellence, impact and implementation quality for a Horizon 2020 call.

assistant: **Abstract**

As sessile organisms, plants have evolved a diverse array of specialized metabolites to respond to their environments. Specialised metabolites are frequently restricted to particular plant lineages, such as the specialised betalain pigments, which are unique to the flowering plant order Caryophyllales - betalains will be familiar as the colour of beetroot. However there are also striking examples of convergent evolution, with the same specialized metabolites emerging independently in different lineages. The host laboratory has recently shown that the betalain biosynthesis pathway has evolved multiple times within Caryophyllales. Underpinning four convergent origins of betalain pigmentation, the central enzyme in the betalain pathway, 4,5-DOPA-extradiol -dioxygenase (DODA), has evolved four times via gene duplication and neofunctionalization. Preliminary data indicate that DODA enzymes from different origins have distinct kinetic profiles, of significance to biotechnology. In the proposed research, I will elucidate the molecular basis underlying the repeated evolution of L-DOPA dioxygenase activity, and resolve the molecular evolutionary trajectories underlying different enzymatic catalytic profiles across different origins of betalain synthesis.  Convergent evolution provides a natural experiment in understanding the genetic basis of repeated emergence and optimization of a metabolic pathway. In exploring the molecular basis of the convergent evolution of high DODA activity, I expect to address many central questions in evolutionary biology, including the respective roles of parallel evolution, epistasis, adaptive constraint, and contingency, in the evolution of molecular complexity.


**Keywords:** Convergent evolution ,  L-DOPA 4,5-dioxygenase ,  Metabolic engineering
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Nano -structural and -dynamic events in the T-cell activation', explaining its relevance to EU policy priorities under Seventh Framework.

assistant: **Impact**

The organization of the T-cell in its resting and activated state, specifically of plasma-membrane molecules involved in the triggering of the T-cell, is a long-standing and contentious research topic in molecular immunology. Understanding the molecular mechanisms involved at the nanoscale, i.e. at spatial scales below the resolution limit of conventional optical microscopy (< 200 nm) will find answers to still open questions, and offer new routes to immunotherapy. The proposed multidisciplinary project aims to bring close together super-resolution STED microscopy and membrane biophysics with molecular immunology. The proposed studies will follow a holistic approach and include the investigation of the functional associations of a multitude of molecules in T-cell triggering, ranging from the T-cell receptor and co-receptors, over other proteins such as kinases and phosphatases, to lipids and the cortical cytoskeleton. Using super-resolution STED and single-molecule microscopy (specifically STED and fluorescence correlation spectroscopy, STED-FCS) I will directly observe, determine, follow and evaluate nanoscale molecular interactions of these molecules from the resting state and early activation of the T-cell until the constitution of the immunological synapse. Besides a sophisticated design of the experimental conditions such as appropriate live-cell fluorescence labeling, biochemical treatments and choice of activating the T-cell, I will improve the STED-FCS technology towards multi-color observations allowing the determination of nanoscale dynamics and interactions of different specific molecules at the same time. With new molecular interactions highlighted, I will be able to better understand how T-cells sense antigen presenting cells and what molecular ramification
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Assessment of trace elements in the gironde's fluvio-estuarine system' to be submitted under Fifth Framework.

assistant: **Abstract**

The project has been designed to provide reliable data on concentrations, fluxes and chemical species of trace elements entering and leaving the Gironde estuary due to different hydrological situations. The existing, well-working long-term monitoring of Cd-pollution along the main tributary will be intensified and completed. It will then monitor-the fluxes of 11 eco-toxic trace elements (As, Sb, Cr, Ni, Cu, Zn, Cd, Hg, Mo, Sn, Pb) into the Gironde by all relevant tributaries, ensuring improved localisation and evaluation of individual sources. Emphasis will be laid on a detailed characterisation of the species of these elements along the complete salinity gradient, considering contrasting hydrological situations and especially the different zones of the stable turbidity maximum. This will provide essential information on the trace element distribution and transformation due to the fundamentally changing milieus in the continuum river-estuary-coast. It will also improve our understanding of mechanisms and processes responsible for the fixation and remobilization of pollutants in these eco-systems. Due to an interdisciplinary scientific co-operation, the project will complete the analytical programme of an eco-toxicity study on the possibility of re-cultivating mussels in the estuary. This co-operation represents the great opportunity to characterise the bio-accumulation of trace elements under real conditions of food production.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'European event on technology technologies for sustainable development""' in the context of a Fifth Framework call.

assistant: **Objectives**

The EET 2001 aims at gathering 1500 European engineers, researchers, public and private decision-makers and offers them two days of multidisciplinary and multi-sector reflection on the theme: Technologies for sustainable development. EETs contribution to this major challenge is to produce original scientific and technical reflection, through 90 speeches and papers, 7 round tables and 10 workshops. These workshops will present 60 technological innovations and foster exchanges of experiences between engineers and between States (EU and non-EU countries). They will provide for each major sector, an overview of the recently achieved or upcoming progress likely to contribute to an economic growth combined with respect for the environment and participate to the reflection on how to encourage conservation of resources and cooperation between developed and developing countries.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Industry structure and the dynamics of competition' to be submitted under Fourth Framework.

assistant: **Abstract**

Very recently, empirical studies have shown that the ranking of industries with respect to the level of firm turnover is highly correlated across countries. That is, some industries tend to have high levels of evoluationary turbulence. while in others the level of firm turnover is low. The aim of my thesis is to explain these differences between industries by investigating theoretically and empirically, the link between industry structure and the level of firm turnover. This project is of great relevance for industrial economics, but it is also important for the (analysis of labour markets, in particular job creation an destruction. The first part of my thesis is purely empirical and consists in carrying on with my previous work by analyzing whether the observed regularities mentioned above still hold at a more desegregated level (four- or five digit). The theoretical literature on industry dynamics has so far failed to make any predictions about the link between differences in industry structure and the differences in the level of firm turnover. In the second and main part of my thesis, I should endeavour to fill this gap in the literature. As a framework, I want to use some existing tractable stochastic industry mode and enrich their strategic environment by adding elements of the literature on industry-specific mechanisms, e.g. the literature on the relationship between industry structure and cost structure, the literature on R&D. innovation and patent races. and the literature on the effects of product differentiation. advertising and brand loyalty on entry and industry structure. Empirical tests of the theoretical results will conclude my project. "
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Ultracold quantum gases in optical lattices with single site addressability', explaining its relevance to EU policy priorities under Seventh Framework.

assistant: **Impact**

Ultracold atoms in optical lattices hold the potential as ideal testbeds for many condensed matter models such as those related to high-Tc-superconductivity. Due to their high degree of purity and regularity, they could also be a candidate for a scalable quantum computation architecture. So far, however, it has not been possible to address individual sites of optical lattices with a sufficiently tight spacing to allow for a substantial nearest neighbour interaction through tunnelling. We propose to implement this crucial feature using a specially designed lens system with a resolution smaller than the lattice spacing.  With this it will be possible to observe and manipulate density, spin structure, and correlations at the scale of a lattice site. We furthermore plan to spatially resolve and to address individual lattice sites in a scalable “quantum register” with 50-100 of qubits in one dimension and later of several thousands in two dimensions. Quantum gates and entanglement between neighbouring atoms can be obtained by collisions in a spin-dependent lattice. The proposed experiment goes far beyond the current state of the art in this field and can only be achieved in a cutting edge research environment. The group in Mainz has emerged as a European and world wide contact point in the field and with the recent addition of Dr. Kuhr, who has extensive experience in the detection of single atoms in dipole traps, the group holds this unique experience.  My personal research skills and abilities in the field of quantum information processing nicely complement the experience of the Mainz group, and together we hope to elevate the  experiments with optical lattices to the next level and put Europe in the forefront of innovation in a field, which could prove industrially viable
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Nontarget analysis of Arctic sediments: An empirical indicator of persistent chemicals overlooked by regulation', explaining its relevance to EU policy priorities under Horizon Europe.

assistant: **Impact**

The increasing volume and type of chemicals produced worldwide every year have the potential to cause severe ecosystem and human health problems. However, due to largely insufficient information regarding the identities, properties, and emissions of these substances, it is challenging to obtain a complete picture of global chemical pollution to aid in development and implementation of effective regulations. Despite the Arctic being pristine and distantly isolated from human activities, Arctic sediments function as a receptor for contaminants that are transported over long distances. Being ubiquitous far away from sources and in the Arctic requires that a chemical is sufficiently persistent, amenable to long-range transport (LRT), and produced in reasonably large quantities. Therefore, it is hypothesized that Arctic sediments can serve as a natural indicator to identify flaws or inefficiencies in current chemical legislation and prioritize persistent chemicals that require further regulation. The overarching aim of this project is to use state-of-the-art mass spectrometric tools together with high Arctic sediments to identify persistent contaminants of global concern, and then apply advanced statistical and modeling approaches to further understand their emission sources and LRT. This analysis will be used to: (a) Prioritize persistent contaminants of emerging concern based on increasing temporal trends recorded in dated sediment cores; (b) Identify major emission regions by analyzing the spatial heterogeneity of contaminants among circum-Arctic shelf surface sediments; (c) Identify dominant LRT pathways by comparing the chemical compositions of Arctic lake sites affected solely by atmospheric LRT versus marine sites impacted by mixed pathways. Results of this project wi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Neural Bases of Multimodal Integration in Children' to be submitted under Horizon 2020.

assistant: **Abstract**

Children typically learn their language in a multimodal environment as their caregivers interact with them with a variety of modalities. Especially, hand gestures are often used by caregivers to convey semantic information with speech. Thus, gestures are an important medium for children to understand speakers’ messages (McNeill, 1992). By using behavioural measures, previous studies have revealed that children and adults can comprehend and integrate information from iconic gestures and speech. For example, the Researcher found that it is hard for 3-year-olds to integrate information from speech and co-speech gestures, but 5-year-olds could perform with similar results to adults. However, behavioural measures do not provide access to the underlying neurocognitive processing of speech and iconic gestures, whereas neuroimaging techniques can provide more direct measures of the online cognitive process underlying the comprehension of co-occurring multimodal semantic information. Thus, this project examines neurocognitive processing of semantic information from gesture and speech in children and adults by using neuroimaging techniques such as EEG and fMRI. Study 1 investigates whether iconic gesture and speech are independently or bidirectionally processed in 3- and 5-year-olds and adults. Study 2 examines which brain areas are used to speech and gesture in children and adults. Two contributions are expected from this project. A practical contribution will be providing information to caregivers and teachers about how they should use their gestures to foster childrens’ language acquisition. A theoretical contribution will be providing neurobiological data on how gesture and speech are processed in children and adults. Through this project, the Researcher will obtain knowledge