RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Developing next-generation genetic reporters for detecting horizontal gene transfer', explaining its relevance to EU policy priorities under Horizon Europe.

assistant: **Impact**

Accurately predicting the spread of genes in the environment via horizontal gene transfer (HGT) is of critical importance to many societal challenges, such as the antibiotic resistance crisis. Our ability to make effective predictions of environmental HGT dynamics is held back by two key knowledge gaps. First, current methods of measuring HGT cannot detect the transient processes that give rise to HGT dynamics, as they can only detect the end result of successful HGT events. Second, mathematical models for HGT spread do not explicitly incorporate these transient processes, limiting our understanding of exactly how these processes affect the spread and mitigation of mobile genetic elements.In this project, SCRIPT: SynthetiC Recombinase-based Investigation of Plasmid Transfer, I propose to resolve these knowledge gaps by (1) developing the first set of next-generation genetic reporters that can reliably detect target transient HGT processes at high-throughput, single-cell resolution, and (2) incorporating these processes into mathematical models of HGT and using these updated models to quantify the extent to which these measurements enhance model predictiveness of HGT spread dynamics and mitigation strategies. This project is an interdisciplinary effort that combines techniques from synthetic biology and mathematical modeling with those from environmental microbiology and HGT research.The outcomes of this project will constitute a major advance in the field of HGT research by enabling the measurement of previously- undetectable processes that are critical in shaping the ecological dynamics of mobile genetic elements. The project will also make an important contribution towards enabling the reliable prediction of the environmental spread of mobile genetic elements carrying
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Hyperoxic MRI of Perivascular Spaces' under Horizon Europe.

assistant: **Concept**

HERMES aims to develop more effective strategies that increase our understanding of the brain in health and disease.The ability of magnetic resonance imaging (MRI) to study brain fluids noninvasively offers a unique opportunity for revealing how cerebrospinal fluid promotes waste clearance from the brain. Perivascular spaces are key structures involved in this process. They are gaps surrounding small blood vessels along which cerebrospinal fluid can move. A comprehensive understanding of the anatomy and function of perivascular spaces is lacking, but it could ultimately uncover important mechanisms of brain disease and lead to novel treatments harnessing cerebrospinal fluid movement for drug delivery to the brain.Building on my expertise in structural MRI and my proposed supervisor’s expertise in functional MRI coupled with supplemental gas inhalation, I will use MRI to track oxygen movements in brain fluids, thus developing inhaled oxygen as an image contrast agent for perivascular spaces. I will characterise the anatomy and function of perivascular spaces in a healthy population from 20 to 60 years old. Then, leveraging my proposed clinical co-supervisor’s expertise in neuroinflammation, I will investigate the role of impaired perivascular space function in multiple sclerosis, the leading cause of neurological disability among young and middle-aged adults, affecting more than 700,000 people in Europe.During this fellowship, I will deepen my scientific expertise by learning how to conduct MRI experiments with multiple image contrasts and supplemental oxygen. The training provided by my supervisors, the scientific and transferable skills provided by the fellowship and the excellence of the host institution will all be essential to deliver the goals of this project. Ulti
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'EUropean RESearch NIGHT- Scientists and researchers uncovered!' to be submitted under Seventh Framework.

assistant: **Abstract**

Researchers’ Night again in Poznan! You cannot miss it! Come and uncover the secrets of scientists and researchers during EURESNIGHT! Measure immeasurable and see invisible! Check what flying kites and photography have in common, become a researcher for one night and construct DNA helix, feed an artificial cow, design your own clothes or simply prepare super fluffy ice creams! Feel like dancing or singing? Come and sing karaoke songs with researchers and at the end dress up like a famous scientist and dance with our researchers cha-cha or jive. Feel like doing some sport? Come and play soccer with our researchers under the moonlight! These are only some of many attractions that wait for you to reveal your passion for science and convince you that researchers are just ordinary people with an extraordinary job.EURESNIGHT is a continuation of the Researchers’ Night projects of the past five years, which were highly successful gathering last year app. 48.000 people willing to meet researchers face to face and to learn new things about their work and personal lives. The project, promoting an image of researchers, builds on last years’ experiences and aims at enhancing further public recognition of researchers and their work by bringing them closer to the public, underlying their important contribution to the daily life and well being of the European citizens. Activities organised during the Night on 28 September 2012 will be focused on public at large and will have a hassle-free and festive character, being a valid alternative to ""traditional Friday night activities"" while allowing learning more about researchers and research. Those activities will offer a direct contact between researchers and public, combine fun and ‘education’ elements (edutainment), try to link togethe
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Shaping Porous Electrode Architecture to Improve Current Density and Energy Efficiency in Redox Flow Batteries', explaining its relevance to EU policy priorities under Horizon Europe.

assistant: **Impact**

The energy transition has increased demand for energy storage, including long-duration storage solutions like redox-flow batteries (RFBs). But RFBs are limited by a high levelized cost of storage, due in part to inefficient electrode use and the lack of tailored RFB components.SPACER will develop high-power-density electrodes for RFBs, with a max. power density of ca. 1Acm-2 and energy efficiencies >85-90% at relevant current densities (20-30% higher than conventional electrodes). The expected cost is up to 50% less than conventional electrodes.SPACER’s approach is the use of hierarchical structures, i.e. complex multilayer materials. Work will entail:• Multiscale modelling to better understand RFB behavior and identify hierarchically shaped pore structures for optimum electrolyte and electric flow• Prototyping of the modelled structures via stereolithic (micro-), 3D printing (meso-) and textile (macroscale) techniques • Characterization of prototypes via cutting-edge imaging techniques like EPR to validate the models and electrode performanceThree development cycles (micro-, meso- and macroscale) will provide insight into complex interactions and optimal material structures, and culminate in electrodes validated in mini-stacks by industrial partner PIN (TRL6). The intended applications are established (vanadium) and next-gen (HBr) RFBs.SPACER will give 17 DCs a unique skill set spanning electrochemistry, modelling, material science and cell engineering. The employability of the DCs will be further enhanced by high-quality individual training in scientific and soft skills, and structured network training units moving them from theoretical investigations toward industrial application. The involvement of 3 industrial beneficiaries and a non-funded Industrial Board, second
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Chemical interrogation of microtubule eraser enzymes' (CHEMTUBIO).

assistant: **Concept**

Erasers of protein post-translational modifications are ubiquitous regulatory enzymes that control cellular function and are targeted for the treatment of disease. One key example are erasers of histone acetylation, where inhibitors have reached the clinic as cancer chemotherapy. Currently, however, multiple other erasers lack tools of study to determine their relevance and generate clinical candidate molecules. One main reason for this limitation is that erasers display multivalent substrate recognition mechanisms that are difficult to replicate with standard assays in vitro and can jeopardise data translation. I have recently developed a solution to detect histone deacetylase activity in situ not on peptides, as it commonly done, but on semi-synthetic protein substrates and complexes, and still applicable to in vitro screening. My preliminary data supports that this methodology can be applied as a more general strategy, enabling studies and drug development on erasers currently inaccessible. Moreover, it holds great potential to localise eraser activity in living systems and generate maps of enzyme action on endogenous biological structures. CHEMTUBIO aims to generalise in situ eraser activity detection through application to the erasers of tubulin tail detyrosination. These enzymes generate gradients of detyrosination across the cytoskeleton lattice through mechanisms unknown and, even though inhibitors are attractive to treat cardiac and neuronal disorders, no probes are available for further investigation. Here, we will address these knowledge gaps and provide in situ sensors and chemical probes to advance the therapeutic application of detyrosinase inhibition. Furthermore, CHEMTUBIO will develop semi-synthesis methods to allow the study of microtubule lumen modifi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'A systems biology approach to investigate cell fate switches in intestinal organoids' goes beyond current state-of-the-art under Horizon 2020.

assistant: **Innovation**

Mass spectrometry-based proteomics and next generation DNA sequencing emerged as two powerful and complementary technologies in biology. I was the first to integrate these technologies in the area of epigenetics to identify and functionally characterize proteins that interact with post-translational modifications on histones and (hydroxy)methylated DNA (so-called chromatin ‘readers’). My pioneering work revealed that an intricate networks of transcription factors, chromatin modifications and chromatin readers orchestrate dynamic gene expression programs during embryonic stem cell differentiation. The next big challenge is to understand the molecular mechanisms, which help to control maintenance and differentiation of adult stem cells as an integral part of an organ. Intestinal organoid cultures recently emerged as a paradigm to study adult stem cell maintenance and differentiation. These ‘miniguts’ can be cultured in vitro and contain all the different cell types that are present in the mouse small intestinal epithelium. Recently it was shown that small-molecule driven perturbations can be used to obtain organoids which are strongly enriched for specific intestinal cell types. This system thus provides a perfect opportunity to study for the first time and in a controlled manner, adult stem cell maintenance and (de)differentiation. Using small molecule-driven perturbations and a unique combination of ‘omics’ technologies, which are embedded in my department, I will provide a systems-wide view of the molecular (epigenetic) mechanisms that orchestrate cell fate changes in intestinal organoids. This integrative approach will identify the major regulatory networks that define the remarkable cellular plasticity of the mouse small intestinal epithelium. Beyond this basic scien
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'PFAS exposure; relationship to serum lipids in children and adults, mechanism of action and potential treatment of high exposed individuals' to be submitted under Horizon Europe.

assistant: **Abstract**

Perfluoroalkyl substances (PFAS) are widely used, persist in the environment and are present in all human populations. Many hot-spot areas across Europe in which humans are exposed through contaminated water or food has raised public concern due to the possible adverse health effects of PFAS. The overarching aim of my proposal is to determine, if PFAS exposure is causally linked to increased serum cholesterol and liver enzymes and if PFAS elimination can be enhanced. Pilot findings from our first ever randomized cross-over trial suggest that administration of the bile acid sequestrant cholestyramine significantly reduces serum PFAS in 34 high exposed individuals, probably by reducing intestinal reabsorption. In work package 1 (WP1) I will explore the promising pilot findings in a rigorously conducted randomized cross-over trial. A trial administering the food supplement psyllium, which also reduces bile reabsorption will be undertaken. PFAS exposure has been associated with increased serum cholesterol and liver enzymes, which pose a major impact to human health due to the causal link to cardiovascular diseases (CVD). It remains unclear whether the associations are causal and mechanisms by which PFAS affect lipid metabolism are not established. In WP2-3 I will study the causal relationships between child and adult PFAS exposure and serum lipids and liver enzymes in high quality prospective cohorts using a novel statistical approach (target trial emulation) to establish causal effects in observational data. In WP4  the mechanisms by which PFAS affect lipid metabolism in human liver cells will be investigated (overview see figure 2). The project will break new grounds for our understanding of the mechanism of action of PFAS on lipid metabolism and the relation with serum c
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Migration and Modernity: Historical and Cultural Challenges' (MOVES).

assistant: **Innovation**

Today’s world sees masses on the move: across the globe, there are almost one billion international and internal migrants; in the EU alone, there are 57 million residents living outside their country of birth, amounting to over 11% of the EU28 population. This unprecedented global situation requires serious political action. Yet this action will only be effective once the historical, cultural and social roots of migration are properly understood. It is this understanding that the ITN MOVES will provide.The project’s chief objective is to undertake a comparative study of the social and cultural roots of mass mobility, and provide the urgently needed historical analysis that can address the so-called migration crisis of the present through an understanding of the population movements of the past. The network has been set up as an interdisciplinary collaboration between researchers in the Humanities and the Social Sciences who will approach migration as both a condition of modernity and one of its greatest challenges, placing the systematic confrontation of past and present forms of migration at the centre of their activities.Through its innovative training programme, carried out in conjunction with 18 non-academic partners (including NGOs, charities, and the cultural and creative industries), MOVES will enable a new generation of experts gain the historical knowledge required to respond to future migration crises with innovative solutions. The project will generate new knowledge about the shaping of the modern world and provide conceptual tools to avoid short-termism in migration management through its emphasis on enduring cultural patterns, historical context, and migration flows over the long term. The links between contemporary and historical migration that MOVES resea
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Molecular studies of the ruminal microorganisms involved in fatty acids biohydrogenation: CLA production' in the context of a Sixth Framework call.

assistant: **Objectives**

Dietary conjugated linoleic acids (CLA) have recently been discovered to have unprecedented health benefits in terms of protection against cancer, heart disease and obesity. These diet-related diseases contribute significantly to EU health-care costs. To c onfer the potential benefits of CLA to humans, CLA rich foods should be included as part of a balance diet. In human the main dietary sources of CLA are ruminant meat and milk, and the synthesis of CLA in the ruminant animal occurs either directly in the r umen or in the tissue from trans vaccenic acid (TVA) formed in the rumen. Both CLA and TVA result as intermediates products of the biohydrogenation of linolenic and linoleic acid. A distinct microbial population is involved in fatty acid biohydogentation w ithin the rumen. To date the production of CLA and TVA within the rumen has been considered solely from the prospective of bacterial metabolism. The objective of this proposal is to apply genomic technologies to improve our understanding of fatty acid meta bolism in the rumen and to feed this information into an ongoing program on the manipulation of fatty acid metabolism in the rumen aimed at improving the quality of ruminant products.Dr Yanez will be based at Institute of Rural Sciences the University of W ales in the laboratories of Professor Jamie Newbold. The project will be based within the Aberystwyth Rumen Microbiology group (ARM). ARM is a research group within the Aberystwyth BioCentre (ABC).This fellowship will help strengthen the European Resear ch area by creating a permanent linkage between Dr David Yanez the researchers in ARM and Dr Yanez colleagues in Spain the proposal will thus ensure a ¿more abundant and more mobile human resource¿ in its research area. Dr Yanez will benefit from a vibrant rese
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Barcoding gene expression dynamics at single-molecule resolution' (DYNOME).

assistant: **Innovation**

Gene expression is an inherently stochastic process. Random expression bursts cause tremendous cell-to-cell variations in mRNA and protein levels. The consequences are beneficial in some instances, e.g., in cell differentiation, and harmful in others, e.g., in bacterial drug tolerance. A key interest in biology is therefore to decipher the kinetics that characterise this noise. What is the distribution of transcription rates in a cell population? Are gene expression dynamics heritable? Do gene networks communicate via the ‘Morse code’ of expression burst? Detailed answers to these questions are pending due to insufficient methods to temporally resolve gene expression noise at single-molecule resolution. A ‘transparent cell’ is needed for which transcription and translation kinetics is accessible for many genes in parallel. DYNOME is my answer to this challenge. DYNOME combines (i) a super-resolution detect-and-bleach strategy, (ii) multi-colour barcoding to monitor up to six genes in parallel, (iii) a lineage tracking tool, and (iv), lab-on-a-chip bioreactors to steer growth conditions. Using this innovative bio-dynamics platform, I will monitor gene expression dynamics at the single-molecule level for many genes in single cells at the same time over many generations. My targets for DYNOME are stochastic decision-making events in bacteria and in eukaryotic cells that include (i) a stochastic phenotype switch (B. subtilis), (ii) the development of non-genetic drug resistance (E. coli), and (iii) cell cycle control (S. cerevisiae). The results will reach far beyond these organisms. Decrypting cell-individuality with kinetic models will be a breakthrough both in basic and in applied sciences with impacts on the development of drugs against bacterial invasions, the design o