RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon 2020 proposal on 'Increasing reproductive success in crops under high ambient temperature'.

assistant: **Concept**

High ambient temperatures represent an obstacle for reproductive development in plants. Our own preliminary data indicate that mutations affecting auxin methylation facilitate pollen tube growth and confer increased reproductive capacity under higher temperatures in the model plant Arabidopsis thaliana. We propose to explore the benefit of reducing auxin methylation in temperature-sensitive crops like tomato and rice to maintain higher fruit/grain yields under heat and drought stress conditions. The research program includes field testing of new tomato varieties generated in this project, in conjunction with a crop company. The combined approach using CRISPR/Cas9 genome editing technology and chemical genetics, together with the thorough training program that we have devised, is targeted at complementing Dr. Resentini’s strong experience in plant molecular and cellular biology. By the end of the term, she will have an advantageous professional position at the interphase between Academy and European Industry.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Multiscale Modeling of Nanostructured Interfaces for Biological Sensors' in the context of a Sixth Framework call.

assistant: **Objectives**

A multi-scale modeling study is proposed to develop a fundamental understanding of the segregation and self assembly of phospholipids and polypeptide amphiphiles at interfaces, and their interaction with liquid crystalline phases. The current study is moti vated by recent experiments that indicate that liquid crystalline phases are capable of interrogating the structure of phospholipidic interfaces at the scale of nanometers, thereby providing a powerful tool for probing nanoscale structure, and providing th e basis for development of sensors for biological molecules. This study is also motivated by experiments from the Stupp group that indicate that peptide amphiphile molecules self-assemble into highly organized structures whose interfaces provide an ideal s ubstrate for cell growth.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Micro and nano deposition' to be submitted under Sixth Framework.

assistant: **Abstract**

Deposition of advanced materials for micro and nanosystems and surface processing (i.e.electrodeposition, electrochemical machining, anodising, PVD, CVD) are a key technology in more than 30% of all industrial production such as corrosion prevention, elect ronics, automotive & aerospace, optics, micro-systems, telecommunication, biotechnology, biomedical sciences. Deposition processes offer various options for a cleaner environment via novel and miniature rechargeable batteries with high energy density, cleanup of soils and water, as well as processing of industrial wastes by membrane separations. EU was leader in this field, but has now an ancillary role versus research carried on in USA and Japan. Although fundamental knowledge in the field is up to now mainly based on EU achievements, the number of patents is now unbalanced in favour of these two countries. EU industry suffers of lack of knowledge on some important production processes. Combination of skills in physics and chemistry, engineering approac hes are needed to enter this interdisciplinary field. The proposed advanced training courses on Micro and nano deposition aim at revitalise EU research by giving to young EU researchers sound fundaments to comprehend the different deposition processes, met hods to approach new scientific and technological problems, overview on the different possible innovative applications; deep understanding of the correlation between the performance of new functional coatings and the structure obtained through controlled d eposition processes; disseminating knowledge on micro and nano defined devices; showing to the participants the variety of combined and integrated solutions available to obtain a functional coating capable to avoid environmental problems. Ultimate goal is to incre
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Identification of the mechanisms of unconventional protein secretion: AP2, a new protein secreted by this unknown pathway' goes beyond current state-of-the-art under Seventh Framework.

assistant: **Innovation**

Most of the secreted proteins transit into and through the endoplasmic reticulum (ER) and the Golgi apparatus. However, eukaryotic cells secrete a class of proteins, which do not enter the ER. Very little is known about this “unconventional secretory pathway”. The last protein identified to be secreted by this pathway is AP2, a cytoplasmic fatty acid-binding protein, which is secreted unconventionally by adipocytes to control glucose liver metabolism. It was reported that the unconventional secretion of Acb1 from Saccharomyces cerevisiae requires diverse group of proteins including GRASP ortholog Grh1, autophagy-related proteins, proteins involved in fusion of membranes with endosomes, members of the ESCRT-machinery, and the plasma membrane t-SNARE Sso1. The requirement of GRASP and autophagy-related proteins has also been reported to be required for the unconventional secretion of IL-1beta, IL-18 and HMGB1 in mammalian cell. How these proteins work together for the unconventional secretory pathway remains elusive. We hypothesize that this process is mediated by a secretory autophagosome and our goal is to reveal the mechanism of this secretory process.We aim to proof this by assessing three main objectives. First, I aim at identifying new proteins require for AP2 secretion. Second, I aim at characterizing morphologically and biochemically the vesicular intermediates required for AP2 secretion. Third, I aim at isolating vesicular intermediates containing AP2 to identify their polypeptide composition.Our approach should allow us to reveal a number of new proteins involved in the process of unconventional proteins secretion as well as the mechanisms of biogenesis, transport and fusion with the membrane of the vesicular intermediates required for this process. The understa
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Clean Heat and Power from Hydrogen' (CLEANER).

assistant: **Concept**

Achieving the European Green Deal target of becoming the world’s first climate-neutral continent by 2050 will require deep cuts to emissions across all aspects of the economy, including the power generation and heating sector. This, combined with the REPowerEU plans, places hydrogen as a clean energy carrier in a unique position. It can be used in, and thereby couple, all sectors like; power&heat, transport and industry. Hydrogen offers long term storage, it can be transported over large distances and it can be produced and used without, or with very low emissions. A central part of the EU climate strategies is the target of domestic renewable hydrogen production of 10 million tons by 2030, in addition to the same amount imported. Large-scale stationary fuel cells in the MW-range should be able to operate on such industrial quality H2 without repurification. They can offer a low-cost clean alternative for both large scale (peak) power and heat production, as well as for small, medium and large-scale back-up power units for the critical infrastructure, thereby also improving the resilience of the energy system. The aim of CLEANER is  to develop and demonstrate for more than 5000 hours a >100 kW PEM fuel cell system operating on industrial quality hydrogen.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Stress as a modifier of atherosclerosis  - Novel mechanistic insights and therapeutic avenues -' (STRATO) targeting Horizon 2020.

assistant: **Abstract**

Atherosclerosis and its complications such as acute coronary syndromes (myocardial infarction and unstable angina) are leading causes of death in the EU and worldwide. Mental stress is known to be a major trigger for the onset of acute coronary syndromes, even in patients with state-of-the-art medical treatment. How acute mental stress rapidly drives plaque destabilization causing acute coronary syndromes is poorly understood and consequently specific treatment, although urgently needed, is lacking. Mental stress is known to affect the immune system. Leukocytes, the effector cells of the immune system, are main instigators not only of plaque progression, but also of plaque destabilization. We hypothesize that acute mental stress rapidly aggravates plaque inflammation, which renders plaques more vulnerable and prone to rupture.We aim to characterize the impact of stress on plaque inflammation in a mouse model of acute mental stress. We will explore the mechanisms by which acute mental stress drives plaque inflammation. Based on these findings, we aim to provide a novel treatment approach to mitigate stress exacerbated plaque inflammation. Further, we aim to translate our findings to stressed humans.The STRATO study will be carried out in a multidisciplinary approach including basic and clinician scientists, immunologists, and psychosomatic specialists and will provide us with an unprecedented, comprehensive picture of how acute mental stress aggravates atherosclerosis. Our study will fill a gap in mechanistic knowledge and based on this will identify novel therapeutic measures with the aim to reduce acute mental stress related cardiovascular complications.


**Keywords:** atherosclerosis, mental stress, inflammation, innate immunity, macrophages, monocytes
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Lipid droplets as dynamic organelles of fat deposition and release: Translational research towards human disease' under Seventh Framework.

assistant: **Concept**

Lipids are central to the regulation and control of cellular processes by acting as basic building units for biomembranes, the platforms for the vast majority of cellular functions. Recent developments in lipid mass spectrometry have set the scene for a completely new way to understand the composition of membranes, cells and tissues in space and time by allowing the precise identification and quantification of alterations of the total lipid profile after specific perturbations. In combination with advanced proteome and transcriptome analysis tools and novel imaging techniques using RNA interference, it is now possible to unravel the complex network between lipids, genes and proteins in an integrated lipidomics approach. This project application of the European Lipidomics Initiative (ELife; www.lipidomics.net) will address lipid droplets (LD) as dynamic organelles with regard to composition, metabolism and regulation. LD are the hallmark of energy overload diseases with a major health care impact in Europe. The project will exploit recent advances in lipidomics to establish high-throughput methods to define drugable targets and novel biomarkers related to LD lipid and protein species, their interaction and regulation during assembly, disassembly and storage. Translational research from mouse to man applied to LD pathology is a cornerstone of this project at the interface between research and development. To maximize the value of the assembled data generated throughout the project, “LipidomicNet” as a detailed special purpose Wiki formate data base will be developed and integrated into the existing Lipidomics Expertise Platform (LEP) established through the SSA ELife project (www.lipidomics-expertise.de). ELife collaborates with the NIH initiative LIPID MAPS (www.lipidmap
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Cost-effective geophysical technology for petroleum exploration in non-seismic areas (CEHEG)' goes beyond current state-of-the-art under Fifth Framework.

assistant: **Innovation**

Objectives and problems to be solved:Significant European oil reserves are contained in geological structures where conventional seismic exploration techniques are largely unsuccessful. Examples of this include Southern Italy, Greece, Albania, and the former Yugoslavian republics. However, complex but prospective structures can be characterized on the basis of electrical resistivity contrasts between the various rock units. An excellent example of this is the over thrust zone of Southern Italy, where the application of magneto telluric (MT) techniques has aided substantially in the identification of the large oil fields of the Val d'Agri. The objective of the present project is to extend existing MT technology to areas where the structures are even more complex, and where the acquisition and processing of magneto telluric data present significant problems.Specifically, issues addressed within the two-year program are:1. Advanced processing techniques, which will function in areas of high industrial noise, and2. Improved sub-surface imaging techniques applicable to near-vertical structures with a strong degree of three-dimensionality. An area in NW Greece will be used as a test area, where limited well control, very poor seismic data quality combine with attractive stratigraphy, on-strike oil production in Albania, and local oil-shows to present an intriguing but technically-difficult oil exploration target.Description of work:The research carried out concerns the validation of a method (i.e. MT) which is cost effective, for characterizing some of the most significant structures, (i.e. near-vertical structures, including salt domes) which could, potentially, host large oil reserves.Examples of the type of petroleum resource assessment issues that are addressed using the
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Enhanced Road Safety by integrating Egnos-Galileo data with on-board Control system' in the context of a Seventh Framework call.

assistant: **Objectives**

The general objective of the ERSEC project is concerned with the broadening of the scope of application to road transport of the EGNOS/GNSS (and later Galileo) through an appropriate integration and data fusion with measurement data coming from other measuring instruments. More specifically, the S&T objective of the project is to develop a measuring system - to be used on board of vehicles - able to output the position on the road map of the equipped vehicle and of all the obstacles (such as other vehicles, peoples and any kind of fixed or mobile objects) around it with a measurement accuracy of the order of 0.1 metre at a sampling rate of 100 Hz.The proposed approach is based on an intelligent datafusion of the EGNOS/GNSS sensor positioning measurement, the Road-GIS digital local map data and the measurement data obtained from an instrument set installed on board of the vehicle, including vehicle dynamic sensors and environmental sensors. Beside the hybridization with other sensors, the optimal use of EGNOS (and Galileo in the future) key differentiators (accuracy, integrity) will be a key factor for the success of the project. This challenging target is required to open the possibility to apply the above positioning data to improve the performance of active road safety systems aimed to avoid collision and off road accidents. Furthermore, ERSEC will open new business opportunities for European companies besides collision avoidance applications, resulting an highly accurate and reliable positioning. The scope of the ERSEC project covers the study and development of a prototype of the ERSEC measuring system and two demonstrators. A vehicle will be equipped with the ERSEC prototype measuring system and a set of trials performed. Furthermore, the ERSEC prototype system wil
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Methyl Donating artificial organelles to support liver cells in Non-alcoholic fatty liver disease' (MetD-AO) under Horizon 2020.

assistant: **Objectives**

Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in the Western world, encompassing a spectrum of liver damage. Multiple issues are involved on the cellular level in failing liver often including enzyme deficiencies such as reduced biosynthesis of S-adenosylmethionine (SAMe). Preserving SAMe homeostasis has only recently started to be considered as a potential therapeutic target in liver-related medical conditions. However, employing the required enzyme, SAMe synthetase (SAMe-synth), as a pharmaceutical, is challenging due to the general issues involved in intact (functional) protein delivery.The aim of the MetD-AO project is to assemble organic SAMe-synth activity mimicking polymer nanoparticles as artificial organelles (AO) and their in vitro characterization of intracellular function in hepatocytes. AOs are typically nano-sized single compartment reactors, aimed to perform a specific encapsulated biocatalytic reaction within a cell to substitute for missing or lost function. The AO will be based on amphiphilic copolymers consisting of a methyl-donating unit, cholesterol methacrylate and poly(5-carboxypentyl acrylate) as membranolytic hydrophilic tail. The latter two will aim at facilitating self-assembly and lysosomal escape, respectively. To allow structurally intact AO to escape the lysosome is unique since typically, the carrier is destroyed and only the therapeutic cargo is release into the cytosol. The proposed AOs with methyl-donating ability are highly advanced because the few prior reported AOs with intracellular activity all considered reactive oxygen related aspects at best. The successful outcome of MetD-AO has the potential to open up entirely new therapeutic opportunities in NAFLD.The complementary expertise of my host Dr
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Targeting metabolic regulation in metastasis formation' (MetaTarGet).

assistant: **Concept**

Distant metastases, i.e. secondary tumors, are the leading cause of cancer deaths. Many patients (especially with breast cancer) are diagnosed when cancer cells have already disseminated to distant organs. Thus, it is of profound importance to prevent the metastatic outgrowth of these disseminated cancer cells into secondary tumors. The ability to remodel the extracellular matrix (ECM) of the metastatic niche is essential for disseminated breast cancer cells to promote their own metastatic outgrowth. This process is to date believed to be transcriptionally regulated. However, the Fendt laboratory has discovered that the nutrient pyruvate metabolically drives ECM remodeling by breast cancer cells. In my project, I will build on this discovery and explore how to target ECM remodeling in metastatic outgrowth. First, I will determine the interaction between the discovered metabolic and the known transcriptional regulation of ECM remodeling. Secondly, I will translate this novel finding into therapeutic potential by defining how to selectively target it, thereby impairing metastatic outgrowth in the lung environment. Thirdly, I will define how the metastatic site and the cancer cell origin affect the discovered metabolic regulation of ECM remodeling, and thus metastatic outgrowth. To address these aims, I will use metabolomics, 13C tracer analysis, genetic engineering and nanotechnology-based drug-delivery in breast cancer 3D cultures and mouse models. With MetaTarGet, I will deliver (i) a mechanistic understanding of pyruvate metabolism as a regulator of ECM remodeling, and (ii) a novel therapeutic strategy to target metastatic outgrowth. This project will allow me to bridge my nanotechnology-based drug-delivery knowledge with the expertise of the Fendt laboratory in metast