RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Intrinsic and extrinsic factors controlling the development of human lung macrophages' in the context of a Horizon Europe call.

assistant: **Objectives**

Human tissue-macrophages demonstrate diverse functions and distinct transcriptomic profiles across organs. They are imprinted by the local tissue environment and contribute to the tissue development, homeostasis, and regeneration. The embryonic macrophages begin to infiltrate the human lung as early as seven post conception weeks (pcw). However, the mechanisms that govern lung macrophage specification and their contribution to human lung development remain poorly understood. This project aims to dissect the cell-intrinsic gene regulatory networks (GRNs), spatial distribution patterns, and signaling pathways regulating the interactions of macrophages and the human developing lung (7–22 pcw). These aims will be achieved by combining single-cell multiomic analysis, high-resolution microscopy, and signaling perturbation using novel 3D tissue models. First, this project will determine the GRNs controlling the identity of fetal lung macrophages by employing multiomic analysis tools. Second, the spatial-temporal maps of macrophages in the fetal lung will be generated using high-resolution light sheet fluorescence microscopy to investigate the distribution patterns and specific cellular niche(s). Lastly, this study will establish novel complex lung tissue models to investigate the underlying mechanisms regulating lung-macrophage interactions. These complementary techniques will expand our knowledge of human tissue macrophage specification and functions, providing insights to the field of both lung and macrophage biology.


**Keywords:** tissue resident macrophage,  human lung development,  human 3D tissue model,  whole mount tissue imaging,  single cell multiomic analysis
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Synthetic photobiology for light controllable active matter' (SYGMA).

assistant: **Concept**

From a Physics and Engineering standpoint, swimming bacteria are a formidable example of self-propelled micro-machines. Together with their synthetic counterpart, self-propelled colloids, they represent the “living” atoms of active matter, an exciting branch of contemporary soft matter and statistical mechanics. Differently from synthetic colloids, however, each bacterial cell contains all the molecular machinery that is required to self-replicate, sense the environment, process information and compute responses. Breaking down these biological functions into basic genetic parts has been one of the greatest triumphs of molecular biology. Today, synthetic biologists are assembling these parts into new genetic programs and exploiting bacteria as computing micro-machines. Project SYGMA will employ the synthetic biology toolkit to provide the building blocks for a light controllable active matter having reliable, reconfigurable and interactively tunable dynamical properties. We will first engineer transmembrane photoreceptors to wire RGB external light signals to cellular physical responses like speed, tumbling, growth and death rates. These genetic parts will allow the modular design of customized active particles to build active materials with unprecedented optical control capabilities. Using these new tools we will address, with experiments and theory, fundamental questions like: how fast can we drive particle density using spatio-temporal motility modulations? what is the force on a body suspended in a bath of bacteria with non uniform motility? how do physical forces contribute to morphogenesis in bacterial colonies? Finding quantitative and experimentally validated answers will eventually allow us to engineer structured illumination protocols to mold living microstruct
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'From flies to humans   combining whole genome screens and tissue specific gene targeting to identify novel pathways involved in cancer and metastases' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

Cancer care will be revolutionized over the next decade by the introduction of novel therapeutics that target the underlying molecular mechanisms of the disease. With the advent of human genetics, a plethora of genes have been correlated with human diseases such as cancer the SNP maps. Since the sequences are now available, the next big challenge is to determine the function of these genes in the context of the entire organism. Genetic animal models have proven to be extremely valuable to elucidate the essential functions of genes in normal physiology and the pathogenesis of disease. Using gene-targeted mice we have previously identified RANKL as a master gene of bone loss in arthritis, osteoporosis, and cancer cell migration and metastases and genes that control heart and kidney function; wound healing; diabetes; or lung injury  Our primary goal is to use functional genomics in Drosophila and mice to understand cell transformation, invasion, and cancer metastases of epithelial tumors. The following projects are proposed: 1. Role of the key osteoclast differentiation factors RANKL-RANK and its downstream signalling cascade in the development of breast and prostate cancer. 2. Requirement of osteoclasts for bone metastases and stem cell niches using a new RANKfloxed allele; function of RANKL-RANK in local tumor cell invasion. 3. Role of RANKL-RANK in the central fever response to understand potential implications of future RANKL-RANK directed therapies. 4. Integration of gene targeting in mice with state-of-the art technologies in fly genetics; use of whole genome tissue-specific in vivo RNAi Drosophila libraries to identify essential and novel pathways for cancer pathogenesis using whole genome screens. 5. Role of TSPAN6, as a candidate lung metastasis gene. Identificati
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Diagnostic test for malaria' (1998-2002), addressing dissemination and exploitation.

assistant: **Impact**

The partners aim to develop a point-of-care diagnostic test using recombinant technology. More specifically, the aim is to develop a test for the detection of antibodies to the malarial parasite using uniquely identified recombinant proteins derivative from the tachyzoite stage of the malarial parasite found in human blood. The diagnostic test format is envisaged to be lateral flow blood test, which will lend itself to low manufacturing costs. Whilst in a European market packaged format the product will be presented through traditional channels, a less sophisticated test presentation will allow the test to be affordable to the primary world areas where the malarial disease is of fundamental economic and health importance. This novel format will require the development of a new test device which will result in a rapid, (less than 5 minutes) accurate, sensitive and specific test which is easy to use and can be developed for further tests.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Possible Stimulation by Mycophagous Collimonas Bacteria of Arbuscular Mycorrhizal Functioning' in the context of a Seventh Framework call.

assistant: **Objectives**

Arbuscular mycorrhiza (AM) fungi are key components in sustainable agriculture as they enable efficient nutrient uptake by crops when chemical fertilization is reduced. AM fungal functioning is, however, influenced by other microbes that interact with their hyphae. One of these microorganisms is the recently discovered genus Collimonas. Collimonas bacteria show a great potential application in sustainable agriculture both as a biocontrol agent and as a P-solubilizer, based on its mycophagy (feed on fungi) and mineral weathering abilities. Here, I propose for the first time, the possible application of AM fungi and Collimonas bacteria as a consortium of beneficial microorganisms for sustainable agriculture purposes. The successful application is dependent on our understanding of the interaction between Collimonas and AM fungi. The aim of this proposal is to gain insights on the mechanisms involved in the interaction, its relevance in natural ecosystems and its influence on plant performance. The Collimonas-AM fungal interaction has both positive (increased hyphal branching, higher supply of nutrients) and negative (consumption of AM hyphae) components. My general hypothesis is that the interaction between both microorganisms commonly occurs in nature and results in a positive mutualistic relationship from which plants benefit. The hyphosphere would provide a beneficial environment for Collimonas growth, which in turn would increase nutrient availability for AM fungi and, consequently, for the plant. To test my hypothesis I propose a highly interdisciplinary approach combining advanced molecular, biochemical and microscopic techniques in natural ecosystems, microcosms and in vitro experiments. The results are expected to bring out new leads for novel strategies in sustain
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Genetic determination of early male parr maturation in Atlantic salmon natural populations' goes beyond current state-of-the-art under Seventh Framework.

assistant: **Innovation**

Understanding the relative importance of short and long term evolutionary processes in species adaptation is central in evolutionary and ecological genetics because it helps understanding how and how fast species respond to environmental changes. In addition, intraspecific variability is of paramount importance for species adaptation to global changes. Atlantic salmon, Salmo salar, provides important ecosystem services but is a vulnerable species spanning its life cycle between the freshwater and the ocean ecosystems, two habitats particularly sensitive to human impacts and global changes. This species is renowned for its life history variability in particular for showing high variation in the age at maturity, especially for male individuals that can mature early before the seaward migration. This phenomenon called early male parr maturation (EMPM) is especially frequent in southernmost salmon populations pointing to an environmental effect on this reproductive behavioral trait. Previous studies had also showed that this phenotype may be under partial genetic control whose precise mechanism remains yet unknown. In the proposed research project GenEarly (Genetic determination of early male parr maturation in Atlantic salmon natural populations) we will combine behavioral ecology, quantitative genetics and population genomics approaches and use the state of the art in sequencing technology to shed new light on the genetic determinism and the genomic architecture of the EMPM phenotype in Atlantic salmon natural populations. The project will produce new understanding about genetic potentialities and constraints for reproductive behavior evolution in face of global changes with a particular focus on improving evolutionary trajectory predictability and conservation practices.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'Mechanisms of kinesin-dependent RNA transport and translational regulation' (2014-2020).

assistant: **Innovation**

Active mRNA transport and localisation are crucial for spatiotemporal control of gene expression. In neurons, dendritic mRNA transport and local translational control are required for synaptic plasticity and memory formation. Misregulation has been linked to severe diseases. RNA-binding proteins (RBPs), kinesin motor proteins, mRNAs and noncoding RNAs (ncRNAs) are involved in dendritic mRNA transport and translational regulation. Still it is not known which essential set of factors is required to enable kinesin-dependent mRNA transport. Further, many RBPs required for mRNA transport simultaneously act as regulators of translation. How these crucial activities are mechanistically linked is unsolved. To anwswer these questions, this project aims to reveal essential mechanisms by two independent biochemical in vitro reconstitution approaches: (1) I will reconstitute kinesin-dependent transport of a dendritically localised mRNA and noncoding RNA using candidate proteins and RNAs from literature and an unpublished result of the host laboratory. (2) I will analyse the dual function of RBPs in mRNA transport and translational regulation. To this end, a microfluidics-coupled in vitro translation imaging assay (TIA) will be developed to study the effect of RBPs and ncRNAs on translation in real-time. Finally, the in vitro motility assay will be combined with the TIA to investigate the impact of translational regulation on mRNA transport. Using these techniques I will address key questions: (i) Which are the essential components required for kinesin-dependent mRNA transport? (ii) How are mRNA transport and translational regulation mechanistically connected? This project aims to unravel fundamental principles, improving our knowledge of neuronal mRNA transport and gene expression
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Central Africa Training Platform for Clinical Research on infectious diseases' to be submitted under Horizon Europe.

assistant: **Abstract**

The world is facing the silent “pandemic” of antimicrobial resistance (AMR). Central Africa has the highest mortality rate (23.5 deaths/ 100,000) attributable to AMR in the world. Research is desperately needed to better understand the burden of AMR in this region, and it is vital that this work is done by local researchers who will be the future leaders of scientific and clinical research in central Africa. The overall aim of the Central Africa Training Platform for Clinical Research on infectious diseases (CATCR) project is to produce essential, world-class research in central Africa, for central Africa, by central African people. To achieve this, it is crucial that the critical mass of PhD and PostDocs in central Africa is increased. Therefore, the consortium will (1) develop a multifaceted training programme on infectious diseases focusing on AMR for MSc, PhD and healthcare professionals; (2) develop a clinical fellowship programme of recruitment, training, evaluation and retention; (3) research the lack of knowledge on antimicrobial usage trends in patients with parasitic infections; (4) research the causal link between prevalence rates and influencing factors of carbapenemase-producing Enterobacterales (CPE) and occurrence of AMR in humans and livestock; and (5) research to understand the prevalence of tuberculosis (TB) and drug-resistant TB characteristics in Central Africa. Finally, (6) the project will focus on communicating, and disseminating research results. Through CATCR, talented researchers, clinicians and health care professionals working on AMR in central Africa will benefit from access to fully-funded fellowships to complete ground-breaking research. Public and regulatory authorities in Africa will use the research and newly established fellowships to
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Stochastic model of on-farm fruit fly behaviour and their response to IPM interventions' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

The purpose of this proposal is to provide financial assistance to an experienced researcher, Dr Slawomir A. Lux, who for 15 years has worked at the International Centre for Insect Physiology and Ecology in Kenya. Currently, he is planning his return to Europe and is looking for re-employment in the research sector in Poland, where he intends to establish his primary and lasting operating base for his future research activities. We plan to utilise resources of the IRG grant for a prompt start of the basic steps of the reintegration process, which would allow Dr Lux to: A. Establish formal ties with the recipient institution (SGGW) through initial employment. Part of the grant resources will be used to complement University contribution to the salary of Dr Lux. B. Undertake his own research under the “umbrella” of SGGW - development of a “Stochastic model of on-farm fruit fly behaviour and their response to IPM interventions”.  C. Expand qualifications through specialised training D. Obtain a formal degree of “Doctor Habilitus” through “habilitation” process, in order to render him formally eligible for an academic position adequate to his experience, and equivalent to the position he has earned in the international system.  E Build a portfolio of his own grants and projects hosted at his new research base at SGGW F. Maintain professional contacts and presence through attending professional conferences.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Centre for materials for low-energy consuming technologies in electrotechnics (MALET)' (MALET), addressing dissemination and exploitation.

assistant: **Impact**

Establishing MALET Centre of Excellence in the framework of this Project is aimed at improving state of knowledge in the field of electro technical materials and technologies. The technological leap will make it possible to economize energy consumption and to protect the natural environment. The latest scientific achievements in this field will be shared and propagated among expert's -scientists and engineers. It will allow for rationalizing transfer of state-of-the-art technologies into the industry, especially to SMEs. This process will be accomplished by organizing Domestic and International Scientific Conferences, meetings of world-top experts, postgraduate trainings as well as placements in foreign research centres and cycles of thematic trainings
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Dynamics of foliated spaces' to be submitted under Sixth Framework.

assistant: **Abstract**

The applicant and the host intend to investigate dynamics and entropy of homeomorfhism groups (resp.,pseudogroups) of fractals. They are going to apply those results for construction of a foliated space of co-dimension 2 and positive entropy, which minimal set is locally homeomorphic to a given fractal (for example the Sierpinski gasket).Moreover, they would like to find what types of minimal sets and entropies can be realised by foliated spaces with restricted geometry. The applicant and the host are going to study relations between the geometry of fractals and dynamics of foliated spaces of higher co-dimension.Results in foliation theory provide information about solutions of partial equations without referring to the explicit form of their solutions. Therefore, one can find applications of foliation theory in many areas of partial differential equations.The knowledge and experience gained during the Fellowship will cause not only individual development of the applicant but also create the possibility to implement new results in science and practical applications of partial differential equations in Europe. Fractals, which can be realised as transversals of foliated spaces, and its dynamics can be applied not only in physics, chemistry or medicine (to describe the growth of cancer) but also for Internet traffic.The joint research with the Department of Mathematics at the University of Illinois at Chicago can increase the competitiveness of Europe in new area of intersection of foliated spaces of higher co-dimension with fractal geometry and dynamical systems. The possibility of implementations of the results of research in industry makes this area of investigations very attractive.