RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Atlas of Cell-Type Specific Nuclear Pore Complex Structures' (NPCATLAS).

assistant: **Concept**

The nuclear pore complex (NPC) is one of the most intricate components of eukaryotic cells and is assembled from ~30 Nucleoporins (Nups) in an unknown manner. Here I present an experimental result driven hypothesis, namely that the composition of the NPC varies across human cell types. As a consequence, the nucleocytoplasmic transport system might be fine-tuned to fulfill specific tasks in various cell types and to adjust the composition of the nuclear compartment. In the research proposed here, cell-type specific structural changes of NPCs will be monitored using systems approaches. The strength of single molecule methods such as cryo electron tomography (cryoET) will be synergistically combined with the strength of mass spectrometry (MS) to measure protein dynamics across cellular states. The outcome will be an atlas of the human NPC containing cell-type and functional state specific structural properties, namely nucleoporin copies per NPC, spatial restraints of protein interfaces, subunit positioning, and shape information. This research will facilitate the generation of a common structural modeling framework by providing the critical information of composition and spatial arrangement. It will furthermore elucidate how NPC composition and structure is adjusted as a function of the biological state of the cell. The long term goal is to integrate the different types of structural data using fitting, docking, and topological modeling approaches in order to project functionally specific compositional data onto structural models of the human NPC.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Diagnostics lab on a chip', explaining its relevance to EU policy priorities under Fifth Framework.

assistant: **Impact**

The problem is increasing demand on healthcare systems, for reasons including the ageing European population and the availability of more diagnostic tests due to the human genome project. For this a rapid, cost effective solution is required. We propose to develop a diagnostics lab on a chip. Lab Chip will greatly accelerate the diagnostics process by miniaturisation and increased detection sensitivity by integrating multiplex molecular biology with dielectrophoresis, Brownian ratchets and micro electro mechanical systems. This will allow several genetic tests to be conducted at the point of care. Cooperative research across Europe will enable this, gathering skills and reflecting healthcare in different member states. The deliverable will be a working chip for chosen diseases (e.g. salmonella) ready for validation and legislative approval. The technology will then be applied to other diagnostics.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Seventh Framework proposal on 'Characterization of protein-dependent biofilms by Staphylococcus aureus'.

assistant: **Concept**

Nosocomial staphylococcal foreign-body infections related to biofilm formation are important problem in modern medicine and S. aureus has become one of the most important bacteria responsible for these chronic infections. The formation of adherent, multilayered bacterial biofilms is the most important factor in the pathogenesis of these infections, which regularly fail to respond to appropriate antimicrobial therapy. Given the impressive array of surface proteins expressed by S. aureus, it seems reasonable that some of them might be involved in biofilm development either in cooperation or independently of PIA/PNAG. Increasing number of evidences suggest the existence of protein dependent biofilm development on clinical isolates of S. aureus.  The general objectives of my research proposal are to identify and characterize proteins involved in biofilm formation. The identification of novel biofilm therapeutic and preventive targets would aid in the development of new and more effective treatments for combating staphylococcal proteinaceous-building biofilm associated infections.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Sixth Framework proposal for the project 'Novel tool for high-throughput characterization of genomic elements regulating gene expression in chordates.'.

assistant: **Objectives**

The aim of this project is to develop open source tools enabling to identify in silico potential cis-regulatory modules, as well as the transcription factors (TF) that bind to them. A publicly available genomic resource describing TF across chordate geno mes, including mammals, fish and ascidians, will be developed. Based on this resource, large-scale sequence comparisons of orthologous non-coding regions of TF genes will be performed. Moreover, a comprehensive in-vitro study of DNA-binding affinity of T F proteins will be used to build novel models of TF binding sites. Multidisciplinary approaches will be developed to identify the cis-regulatory regions driving TF gene expression. The basic steps undertaken will be: 1.Genome wide census and phylogenetic analysis of all TFs in sequenced chordate genomes. 2.Genome wide identification of Multi Species Conserved Sequences (MCSs) within orhtologous regulatory regions of chordate TFs. 3.Characterization of the activity of the identified MCSs in Ciona and zebra fish embryos, mammalian cells as well as in transgenic mice. 4.Training of a novel algorithm to predict and characterize MCSs active in various model systems 5.Comprehensive determination of the in vitro DNA-binding specificity of all transcription factor s in a chordate genome 6.Building novel bioinformatics models of TF binding sites based on complex grammars such as Hidden Markov Models and Stochastic Context Free Grammar 7.Integration of the above data into a publicly available, open source bioinformat ics tool that can be used either via the project website or downloaded for large-scale projects This project is a large-scale pluri-disciplinary effort to decipher the grammar of chordate regulatory sequences,and will have a strong impact by building too ls and resou
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Horizon Europe proposal for the project 'A Darker Shade of Whiteness: The Italian Ethnic Press in Louisiana and the Making of Racial Awareness in The Gulf South (1877-1945)'.

assistant: **Objectives**

The stereotypical representations of Italians as racially inferior and dangerous for public security saturated the American public discourse at the end of the Reconstruction (1877) and tragically materialized in 1891 when 11 Italian Americans were lynched in New Orleans.  The racial landscape of the Crescent City is exemplary of the Italian migrants’ struggle for acceptance across the South and can be fruitfully used as a testbed to study the intertwining of diaspora and race both in the Gulf South and nationally. Through the study of the public discourse emerging from rare and still understudied Italian-language ethno-cultural periodicals published in Louisiana and widely distributed outside the state, DaShoW illuminates how the shifting discourses on Africans, African Americans, Native Americans published in the Italian-language periodical press contributed to ‘manufacture, assert and defend the Italian race’ within one of the largest diasporic Italian communities in the United States. The project will demonstrate how the racialization of Southern Italians in New Orleans and Louisiana was the result of the appropriation of stereotypes rooted in the North/South post-Risorgimento divide transposed from Italy and rhetorical strategies ascribable to the Italian Positive School of Criminology. DaShoW will study the rhetorical and practical strategies through which the Italian communities of Louisiana and the Gulf South negotiated their racial standing between 1877, which ended the Reconstruction thus paving the way for the deprival of African Americans’ voting rights, and the formation of an early Southern civil right movement (1945). DaSHoW promotes a comprehensive framing of the Italian diasporic presence in the racialized geographical, social and cultural context of the
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Top amplifier research groups in a European team' in the context of a Sixth Framework call.

assistant: **Objectives**

The aim of TARGET is to overcome the fragmentation of European research in the field of microwave power amplifiers for broadband wireless access by creating a progressive and durable integration of research capacities of the network partners. Ultimately, European technology and research in the fields of active power devices beyond CMOS, of the characterisation and simulation of materials and devices, of amplifier design and linearisation, and in the field of broadband transmitter system design shall attain a leading role in the world. This challenge has been rightly acknowledged by the European Commission in making  Pushing the limits of CMOS and preparing for Post-CMOS" a strategic objective of the IST work programme 2003-2004.TARGET will bring together 45 top amplifier research groups consisting of 168 researchers and 67 doctoral students (coming from 12 EU member countries, 3 candidate countries, and 2 associated countries) that have in the past three years published more than 1000 papers. This integration will be pursued with the aim to - create a co-operative network- establish a virtual centre of excellence- stimulate and co-ordinate world-class research.TARGET will follow a well-designed methodology to integrate research in this field. As a general principle, it will use research itself for the purpose of achieving a desire to integrate among the participants. A total of 26 work packages in the fields of integration, joint research, dissemination of knowledge, and of network management are defined for the first 18- month period. To account for the special needs and interests of female researchers a gender action plan is foreseen.Over the four years funding period an average funding rate of only 2,000,000 per year is requested. To prepare for time after community
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Seventh Framework proposal on 'Urban, peri-urban and regional food dynamics: toward an integrated and territorial approach to food'.

assistant: **Concept**

Increasing urban food-related health and environmental problems are raising an urgent need for action. Current agrifood studies tend to neglect the broader societal and spatial impacts of food. PUREFOOD will reduce the high knowledge and skills deficit that negatively affects the capacity to deliver political and developmental solutions related to food security, public procurement, health and sustainable regional development. Through its innovative methodology, the interdisciplinary courses, private and public sector involvement, the holistic conceptualization of sustainable food and the formation of Communities of Practice (CoPs) that include actors at all stages of the food chain, PUREFOOD contributes to Commission’s aim to deal with economic, social and environmental policies in mutually reinforcing ways. We study an alternative geography of food, based upon three emerging trends; sustainable food supply chains, public sector food procurement practices and (peri-)urban food strategies. Each is topic of a scientific WP with 4 ESRs in individual research projects using case study methodology. The related training aims to transfer disciplinary scientific knowledge and skills between complementary groups, leading to a coherent frame of basic and advanced scientific, professional and host institute training modules, and to interactively develop scientific and professional knowledge and skills through ‘learning-by-doing’ in CoPs for each WP. PUREFOOD consists of 7 full academic partners and 8 associated partners (private sector and socio-economic partners). PUREFOOD’s coordinator has ample experience in managing international interdisciplinary research and training projects. The commitment of private and public organisations and NGOs as associated partners illustrate the t
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Understanding and managing soil health impacts to protect soil-dependent pollinators' to be submitted under Horizon Europe.

assistant: **Abstract**

ProPollSoil aims to enhance our understanding of the relationship between soil health and soil-dependent pollinators, which spend part of their life cycle in or on soil and play vital roles in ecosystems and agriculture, providing services like pollination, decomposition and nutrient cycling. We specifically focus on lesser-known species and groups, such as ground-nesting bees, wasps, hoverflies, ants or beetles. ProPollSoil will identify how intensive soil management practices and contamination, threatening both pollinator and soil health, contribute to the decline of soil-dependent pollinators. This knowledge will be used to inform sustainable soil practices and guide conservation and restoration efforts. We will (i) conduct a pan-European field sampling campaign, including SOB4ES sites, (ii) develop novel monitoring methods, including eDNA and AI, (iii) perform lab studies to fill existing knowledge gaps, such as the role soil microbiota, (iv) assess intensive management, soil compaction and soil pollution as potential soil and pollinator threats within a co-creation research approach in nine Case Study Areas (CSAs), and (v) use different modelling approaches to produce EU-wide maps of soil substrate availability and hence pollinator suitability. Our multi-disciplinary team involves experts in soil science, pollinator ecology and biology, biodiversity monitoring, big data, social sciences, modelling and ecotoxicology.By contributing new knowledge and data on soil health and pollinator diversity, maps on pollinator and soil habitat quality and novel approaches for joint soil and pollinator monitoring to the Mission “A Soil Deal for Europe” and the EU Pollinators Initiative, “A New Deal for Pollinators“, ProPollSoil will support strategies for reducing soil pollution,
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'An accoMpanying mEaSuRe for European Commission's IT nodes actions in the areas of tecHnologies for business processes, electronic commerce, multimedia content and tools and web technologies' (HERMES), addressing dissemination and exploitation.

assistant: **Impact**

The aim to accompany the IT Nodes activities of the European Commission by developing demonstrators and organizing workshops in the selected areas, covering Technologies for Business Processes and Smart Organizations, Knowledge Management, Electronic Commerce, Multimedia content and tools and WWW technologies. The results of highly successful previous European Commission supported projects will be used in developing the demonstrators, that is, inter-enterprise workflow system developed within the INCO-DC 97 2496 MARIFlow project will be used for TBP and the result of OPENISE (IST-1999 11590) and CATCH 2004 (IST-1999 11103) will be used for the provision of a prototype based on a tool will for processing multimedia content for a cultural heritage. Finally, in the context of this project three workshops with tutorials and technical papers will be organised covering the aforementioned project target areas and providing information on IST proposal preparation.Objectives:The information technology take-up in Mediterranean countries requires more efforts to complement the IT Nodes activities; the advanced technology in well targeted areas should be explained and demonstrated through concrete demonstrators and training, in forums that include the industry, the end users, software developers, business organizations and the academicians from these countries as well as from EU. HERMES project will achieve these objectives in the areas that are the intersection of IoT and IT Nodes activities, that is, Key Action II and Key Action III, which cover Technologies for Business Processes, Electronic Commerce, Multimedia Content and Tools and WWW technologies. Three workshops covering the above areas will be organised, containing tutorials and technical papers.Work description:HERMES pro
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'New types of drugs for cardiovascular system, based on its regulation by atp via p2' under Fourth Framework.

assistant: **Concept**

Foreseen ResultsIn this project a novel approach of effect-directed drug design in used to fill this gap. This approach is based on a non-exclusive model of agonist and antagonist interaction with receptor; its combination with quantitative structure-activity analysis allows prediction of the type of ligand action (agonist, antagonist or partial agonist). In this project we will express a series of the recombinant P2Y receptors in cell lines, in which the activation of these receptors is coupled to intracellular Ca2+- mobilization. The dose-response relationships of receptor activation can thus be determinated by means of fluorescence measurements to detect Ca2+mobilization. This assay system enables rapid screening of ligands and will be used for systematic studies of ligand-binding specificity of these receptor subtypes, analyzed proceeding from the modified receptor concept. Following the results of these studies new types of purinoceptor ligands will be developed, including nucleoside-coupled peptides. Screening of the activity of these ligands will be made by using synthetic combinatorial libraries, based on randomization of either the peptide or nucleotide parts of these compounds.A regulatory function of nucleotide (ATP, UMP,ADP, etc), interacting as extracellular transmitter through specific nucleotide receptors (P2 purinoceptors") has been discovered recently and was finally established by the DNA-cloning of some of these receptors. It is becoming evident that purinergic receptors may present a novel amplification mechanism, strengthening the cellular responses in situations of crisis. This is exemplified by the platelet aggregation reaction where release of nucleotide (ADP) from one platelet activates and recruits hundred of other platelets, thus rapidly initi
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'Single-spin magnetic resonance spectroscopy and imaging', explaining its relevance to EU policy priorities under Horizon Europe.

assistant: **Impact**

Magnetic resonance spectroscopy studies matter by probing the resonant frequencies and couplings of paramagnetic nuclei and unpaired electrons. It is a cornerstone of modern science, with numerous applications in condensed-matter physics, materials science, chemistry, biology, and medical imaging. A counterpart is low sensitivity. Conventional spectrometers need large numbers of identical objects to detect a signal. This is an issue for systems with inhomogeneous properties, since their ensemble linewidth is then larger than the linewidth of the individual objects, which limits spectral resolution. The vision of ONESPIN is to apply magnetic resonance spectroscopy to individual objects, based on a new method recently developed in my laboratory. We detect individual electronic paramagnetic centers at 10mK by counting the fluorescence microwave photon emitted when they relax radiatively to the ground state, using a single-microwave-photon detector based on a superconducting qubit. The first project objective is to apply this fluorescence detection method to a large variety of individual paramagnetic centers (organic radicals, transition-metal-ion-containing molecules, enzymes, catalytic centers), which will yield the first single-center-resolved spectra on these systems. The second objective is high-resolution spectroscopy and imaging of the nuclear spins surrounding an individual paramagnetic center by combining hyperfine spectroscopy with fluorescence detection. We will obtain the first magnetic resonance images of individual molecules with single-nuclear-spin resolution, a long-standing dream of magnetic resonance. The third objective is magnetic resonance imaging with 10nm resolution of individual paramagnetic centers in a micron-scale sample by combining magnetic grad