RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Interdependence between cell adhesion and proliferation in normal and neoplastic cells' (1994-1998) under Fourth Framework.

assistant: **Objectives**

We plan to analyze the reciprocal interactions existing between cell adhesion and proliferation. These interactions are suggested by phenomena such as the anchorage dependence" for G1 to S transition in the cell cycle and "contact inhibition", which are commonly dysregulated in cancer cells. We will analyze in vitro models represented by normal, pre-transformed or neoplastic cells, arrested in GO/G1 by starvation and contact inhibition and subsequently stimulated by adhesion to matrix or specifc antibodies or kept in a non adherent state. Transcripts whose levels are selectively controlled by any of the aforementioned conditions will be differentially analyzed using the "Differential Display of RT-PCR products" (DDRT-PCR) approach, and subsequently cloned for complete characterization. The role of functional cyclin/cyclin-dependent kinase(CDK)/ cyclin inhibitor (CKI) complexes in affecting the adhesive phenotype will be explored at the biochemical and functional level, using synchronized cell populations at defined stages in the cell cycle. The long term goal of the project is to identify early mutational events affecting genes whose expression controls the interdependent functions of adhesion and growth reponse, and whose alterations correlate with tumor progression. "
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Format-Agnostic SCript-based INterAcTive Experience' (FascinatE), addressing dissemination and exploitation.

assistant: **Impact**

FascinatE will create an innovative end-to-end system and associated standards for future immersive and interactive TV services. It will allow users to navigate around an ultra-high resolution video panorama, showing a live or recorded event, with matching accompanying audio. The output will be adapted to their particular device, covering anything from a mobile handset to an immersive panoramic display with surround sound, delivering a true personalized multi-screen experience.At the production side, this requires new scene capturing systems, using multiple microphones and cameras with different fields-of-view and frame rates. These various video signals, together with metadata describing their relative alignment, constitute a novel layered scene representation. From this, any particular portion can be rendered at any desired resolution. This represents a paradigm shift in production technology, from today's format-specific view of an area selected by a cameraman to a format-agnostic representation of the whole scene. This approach is considered to be a more intelligent and future-proof alternative to other approaches, which just increase the resolution of the pictures (e.g. to 8k).Script metadata will describe shot framing as suggested by the supervising director. Rule-based systems will frame these regions in a subjectively-appealing manner, taking into account knowledge of how to adapt them to different display sizes, as well as the personal preferences and interactions of the user.Intelligent network components will tailor the transmitted data to suit the screen size and selected view for each terminal. For low-power devices, the component itself will render the desired view, whereas for powerful devices, better performance will be achieved through selectively trans
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the novelty, innovation potential and advance beyond the state of the art for 'A biomimetic zonally gene-activated load-bearing reinforced hydrogel for meniscus repair' (GEMGEL).

assistant: **Innovation**

Meniscal tears are the most prevalent injuries of the knee joint and a major contributing factor to osteoarthritis (OA) development. Current treatments cannot effectively repair meniscus and only delay the onset of OA. Tissue engineering and regenerative medicine (TERM) approaches aim to promote the regenerative capacity of the tissue; however, this is hindered by the challenges of replicating the complex microenvironment of the tissue coupled with its intrinsic avascularity. My overarching aim is to develop an innovative zonal load bearing scaffold through melt electrowriting optimised using a machine learning approach to mimic meniscus biomechanics and extra cellular matrix (ECM) architecture. This will be integrated with corresponding zonal gene activated hydrogels using transcription factors implicated in meniscus regeneration to induce intrinsic regenerative capabilities. Prof. Khademhosseini (Terasaki Institute; outgoing phase), has extensive experience in hydrogels, 3D printing and stem cells. I will develop a 3D layered microfibre reinforced hydrogel scaffold mimicking the collagen architecture, biochemical and biomechanical properties of the meniscus and test its biological performance using human stem cells. Prof. Fergal O’Brien (RCSI; return phase), has extensive experience in scaffold-mediated gene therapy, particularly for musculoskeletal applications. Using techniques established in his group, I will develop zonally gene-activated scaffolds that can be used as a local delivery system in a radically new regenerative therapy for meniscus tears. Under the supervision of Prof. O’Brien and Khademhosseini, who will guide my Career Development Plan, I will develop key complementary skills in project management, entrepreneurship, commercialization, dissemination a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Aligning Life Cycle Assessment methods and bio-based sectors for improved environmental performance' goes beyond current state-of-the-art under Horizon Europe.

assistant: **Innovation**

The ALIGNED project will deliver a modelling framework to assess and optimise the environmental and socio-economic performance of bio-based industries. ALIGNED will advance the scientific field of Life Cycle Assessment (LCA) (moving from TRL2-3 to TRL5) and collaborate with industries and representatives from five bio-based sectors: construction, woodworking, textile, pulp and paper, and bio-chemicals. The transition towards a sustainable economy is dependent on consistent and comparable environmental assessments of bio-based products. However, in practice today the methods to assess the impact of bio-based products give incomparable results, thus confusing decision-making. The models and tools developed in ALIGNED will allow to perform high-quality assessment studies across the bio-based sectors, with industrial relevance and interoperability. This is possible by the iterative application and improvement of the new and harmonised models and tools in six specific cases of bio-based industrial technologies (TRL 2-6).The ALIGNED framework will allow accurately to model key aspects not covered in current practice: the competition for biomass and for land, dynamic and time-specific carbon accounting, and biodiversity and socio-economic impacts. ALIGNED will also develop future energy and resource scenarios derived from integrated assessment models, and a consistent approach to uncertainty assessment. Key stakeholders in the five sectors will be continuously involved, by providing feedback in the early framework development and by sharing the learnings from its practical application. The professional engagement of stakeholders will secure industry relevance and acceptance delivering real impact.


**Keywords:** Life Cycle Assessment; Sustainability; Global Warming Potential;
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon 2020 proposal on 'Competitive assembly dynamics of the DCC receptor with its guidance cues integrates signals for cellular steering'.

assistant: **Concept**

Secreted proteins and their receptors underlie the communication networks of multicellular organisms and guide the proper organization of developing tissue. An essential process involves the human transmembrane receptor and signal integration hub, Deleted in Colorectal Cancer (DCC). Emerging evidence indicates that the netrin and draxin guidance cues are secreted by neighboring cells and form competitive and complex multivalent interactions with DCC. The DCC receptor thus integrates multiple inputs (netrin and draxin) to elicit the appropriate physiological response (cell migration towards or away from the source of netrin or draxin, or even programmed cell death), resulting in proper connectivity of neurons in the brain and of endothelial cells in blood vessel networks, or their misprogramming in tumorigenesis. Our knowledge of this medically important signaling pathway is now poised for translation to an integrated systems mechanism across biological scale. This research action will address the challenge by experimentally determining the competitive and multivalent assemblies formed among netrin, draxin and DCC as a biophysical basis for signal integration. These binding events will then be computationally simulated to examine their dynamic partner exchange and polarized orientation of assemblies in the membrane that are dependent on their distance from the netrin and draxin sources. Experiments and simulations of competitive binding and assembly will be further complemented and validated by assaying the distance-dependent turning response of living axons placed between netrin and draxin-soaked beads. Together, these approaches will describe the competitive assembly dynamics of signaling and will provide unprecedented mechanistic insights into cellular communication a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Understanding and combating human age-related muscle weakness' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

Ageing of skeletal muscle results in a progressive loss of mobility that decreases the quality of life and has major economic and social consequences for society at large. Increasing muscle weakness is a major component of muscle ageing. In the elderly muscles become atrophic (loss in muscle mass) and weaker (loss in muscle force), more susceptible to damage and consequently regenerate and recover more slowly than was the case in their youth.  The challenge for FP7 is to identify the relative importance of sarcopaenia, thus proposing standards to define healthy ageing in order to identify age-related muscle weakness; secondly to identify molecular pathways which may be targeted to combat normal age related muscle weakness, and thirdly to identify therapeutic strategies to prevent muscle loss and weakness and enhance recovery following injury or immobilisation.  The approaches which we will take to understand and combat muscle weakness in the aged population and improve healthspan can be defined in several steps: the collection and collation of data and samples, the assessment of physiological and functional parameters, the understanding of the various biological mechanisms involved, leading through integration to the development of strategies and their translation for the general European population. In order to develop and propose the general public with efficient countermeasures, the consortium will integrate data from genetics and epidemiology, molecular and cellular biology, physiology, biomechanics, as well as clinical and public health aspects, to ensure optimal scientific synergy from the leading European specialists and companies.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write clear, measurable objectives and define the scope for the project 'Proof of Concept for Rethinking Tunnels in Urban Neighbourhoods' (RETURNPOC) under Seventh Framework.

assistant: **Objectives**

This proposal will develop cutting-edge technology to better link two rapidly growing industries:  laser scanning (aerial and terrestrial) and three-dimensional printing. The solution will circumvent the current methods, which rely upon an intermediary Computer-Aided Design programme. By direct transformation of the data points into printable surfaces, greater levels of automation can be achieved, while producing superior results. Furthermore the intended solution will enable the integration of previously disregarded semantic data such as colour and intensity. For architecture, civil engineering, and urban planning,the resulting technology will offer the immediate benefits of an order of magnitude reduction in time and cost. The proposed innovation is enabled by the immediate access to extremely high quality aerial and terrestrial laser scanning that was collected through the original ERC RETURN grant.""
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'Safe chemical analysis of high temperature metals using pulsed radar' under Fourth Framework.

assistant: **Concept**

The knowledge of the chemical composition of metallurgical components has become of increasing and sometimes v ital importance. The behaviour and effects of. for example, the heat affected zone adjacent to welds, joins in dissimilar metals. corrosion products. overheating and embriulement all depend critically on chemical composition (e.g. metallurgical phase changes during embrittlement). With existing, older equipment, chemistry is often unknown. In nuclear industries the plant is likely to be radioactive and cannot be accessed. Current methods of industrial analysis are testricted to cutting out material from the metal and submitting it to more traditional chemical assay. However. this approach has severe limitations on highly stressed high temperature components. Tt is the proposal cf this project that it is possible to make laser spectroscopy practicable and viable for use as a remote industrial diagnostic tool by specifically addressing its limitations and applying new and innovative technologies to overcome them. The project intends to develop the following in order to achieve this goal: * Advanced sig noise and data analysis systems to obviate the current limitation on the temperature of the component being analysed (up to component te the range 500<°C<1OOO). * Automatic electronics for control of positio (where current systems use manual methods which greatly increases its complexity of use whilst limiting its reproducibility). * Improved so (much current software. in conjunction with the spectroscope. limits the resolution to which the spectrograph can separate emission lines. Cu software is also particularly user-unfriendly). * Selective spectrometers. * Tuneable lasers for particular spectral line excitation. Until now such an approach has not been taken to
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'State of the Art and Innovation' section for a Horizon 2020 proposal on 'Investigating inflammatory signaling by combining phospho- and ubiquitin proteomics with CRISPR/Cas9 technology'.

assistant: **Innovation**

Deregulation of pro-inflammatory cytokine signalling is the underlying cause for many inflammatory diseases. Thus, understanding the molecular mechanism of cytokine signalling is necessary to develop suitable therapies. The enormous complexity of cytokine cascades has however impeded the development of new therapies. To shed more light into these intertwined signaling cascades, I plan to use a proteomics approach that allows the identification of signalling mechanisms in a multi-dimensional and unbiased fashion. The recent development of the EasyPhos protocol in the Mann laboratory will allow me to take an unprecedented look at phosphorylation events occurring in cells stimulated by single and by combinations of disease relevant cytokines. Simultaneously I will be able to detect changes in the ubiquitinome using the di-Gly enrichment strategy. By combining phosphoproteomics and ubiquitin proteomics with the CRISPR/Cas9 technology to delete and modify members of diverse signaling pathways, I will be able to analyze the role of these players and their post translational modifications in the respective signalling pathways. This study will therefore investigate the mechanisms and the relationship between phosphorylation and ubiquitylation in cytokine signalling in an unprecedented way and has the potential to result in the identification of new therapeutic strategies for the treatment of a range of inflammatory diseases.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Fourth Framework proposal for the project 'Ultraviolet radiation in the Arctic: past, present and future.'.

assistant: **Objectives**

a) To study the clear-sky and cloudy-sky UV trends. b) To study the relative importance of various factors on UV irradiance at ground level. c) To improve the parametrisation of radiative transfer models as based on UV and ancillary measurements. d) To develop UV-B scenarios for Europe as based on ozone scenarios taking into account increase of atmospheric CO2 concentration.The UVRAPPF project contains following research tasks:  1. Past changes in solar UV-B radiation in Northern Europe  2. Processes affecting UV-B radiation in Northern Europe  3. Parametrisation of radiative transfer models  4. Development of UV-B scenarios  The first task will be realised by using up to 20 year time-series of quality-controlled broadband UV-B  measurements, up to 45 years of total ozone measurements, other radiation measurements and radiative transfer  models. Special emphasis has been paid on the quality control of UV records at the stations selected for the  UVRAPPF project.  The second task will be realised by studying several years of routine, quality-controlled spectral UV  measurements performed at 8 stations in Northern Europe. The vertical distribution of ozone, aerosols and  meteorological parameters, records of total O3, SO2 and NO2, measurements of solar radiation, clouds and  albedo, performed for several years at the stations will be used for studying the effect of various factors in  affecting UV-B irradiance. These processes will be partly studied with DISORT and UVSPEC radiative transfer  models. The results of these studies will further be used for improving the parametrisation on the radiative  transfer models.  Finally, UV-B scenarios for the next century for Europe will be produced by using the newest stratospheric  ozone scenarios, as produced by the leading strat
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the 'Expected Impact' section for the project 'Confocal impedance microscopy of cell interactions with surfaces' (CELLSPIM), addressing dissemination and exploitation.

assistant: **Impact**

A confocal impedance microscope capable of producing two-dimensional electrical images and three-dimensional optical images of materials and cells simultaneously will be developed. The electrical images are produced using two closely related techniques. In formation about the local electrical impedance will be obtained using Scanning Photo-induce Impedance Microscopy; information about local electrical potentials and pH will be obtained from so-called Light-Addressable Potentiometric Sensors. Both techniques are based on photocurrent measurements at electrolyte-insulator semiconductor structures. Optical images will be measured by confocal microscopy.Electrical and optical images will be used to monitor physiological processes such as signal transmission in neurons or metabolic processes. Using the new instrument, ion channel activity will be monitored in different parts of the cell at the interface between cell and surface, an area that is not accessible by traditional patch clamp techniques. For the design of new materials that promote tissue growth, the interaction between cells and surface is of great importance. The confocal impedance microscope will provide information about cell morphological and cytoskeletal changes and also changes in the impedance, i.e. transport properties of the cell membrane in the cell attachment area.