RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Building a reproducible single-cell experimental workflow to capture tumour cell persistence' (PERSIST-SEQ).

assistant: **Concept**

It is the ambition of PRESIST-SEQ to provide a new gold standard in single-cell experimental workflows the cancer research community by developing best practices, standard operating procedures (SOPs), and high-quality FAIR data, with the ultimate aim to empower them to unravel therapeutic resistance. Such, that the community can identify urgently needed markers to predict, prevent, and target tumour resistance. Cancer takes 9.6 million lives each year, 90% of which result from untreatable metastatic relapse occurring after initially (seemingly) effective treatment. Therapeutic resistance is hence a primary cause of cancer death that clinically cannot be predicted, prevented, or treated. Addressing the urgent need for smarter therapeutic strategies is however held back by the lack of standardised experimental approaches that enable studying the biology of residual disease and drug tolerant persister cells in full detail. This need encompasses best practices for single-cell sequencing, advanced modelling techniques using patient-derived organoids and xenografts, and data FAIRification for integrated experiments. To address this need, PERSIST-SEQ brings together globally leading groups in single-cell sequencing technologies, cancer modelling and therapeutic resistance. Furthermore, the consortium has a broad range of clinical samples, cell lines, 3D models (PDX and PDOs) and mice models (GEMMs) at its disposal that can be leveraged to answer a broad range of emerging questions. This positions the consortium excellently to (1) design and standardise single-cell experimental approach to study the biology of therapeutic resistance and (2) initiate the largest single-cell profiling initiative on therapeutic resistance. Importantly, PERSIST-SEQ is organised such that it can qui
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Novel anti-inflammatory compounds for autoimmune diseases' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

The consortium has a lead series of p38 inhibitors with unusual anti-inflammatory properties.     The drug leads were originally elaborated as a potential therapy for inflammatory bowel disease, however, the potency of the compounds and their general anti-inflammatory mode of action has raised the possibility of studying their utility in other diseases including rheumatoid arthritis.  In addition, feedback from licensing partners has indicated that clear activity in a range of other disease models would dramatically increase interest. A key issue in bringing substances of this class to the clinic is variable patient response to p38 inhibitors in previous trials.   Data to date have indicated that there is a potential groups of responders for whom therapy with this class is highly beneficial.  The effect of non-responders has, however, made trial outcomes to variable to warrant further development.  To solve this problem, we intend to further extend proof-of-concept in patient stratification to allow us to select responder populations for initial patient trials.  The objectives of the project are, therefore: § To prepare analogs of the existing compounds § Conduct pre-clinical models for rheumatoid arthritis, IBD, sepsis, liver fibrosis and motilin dependent gut stimulation § Examine the activity of the compounds in human tissue explant models § Select a pre-clinical candidate class § Develop a scaled-up process for GMP synthesis of the lead § Conduct a survey of p38 expression in IBD and RA sub-types at various stages of disease and correlate this plasma markers. § Based on results of tissue explant studies and p38 expression, select a patient stratification protocol for phase 2A studies in man.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write the concept and methodology overview for 'StemSight: curing blindness with stem cells' under Horizon Europe.

assistant: **Concept**

StemSight is developing off-the-shelf stem cell therapies to cure blindness. We aim to solve the global shortage of corneal donor tissue by producing high-quality tissue products using allogeneic induced pluripotent stem cells (iPSCs) and biomaterials. Our lead product is aimed at a rare but severe form of corneal blindness, limbal stem cell deficiency (LSCD), for which there are currently no valid treatment options. The company's innovations have arisen from extensive academic research, and the company has spun out from Tampere University, Finland in 2021. StemSight is a rare kind of deep-tech startup, as all of its scientific founders are women.


**Keywords:** Regenerative medicine,  Stem cells,  Blindness
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Fifth Framework proposal for the project 'Mechanisms of ageing in extracellular matrices (AGEING ECM)'.

assistant: **Objectives**

A major aspect of the ageing process in the human population is the loss of suppleness of extra cellular matrix tissues (such as skin, tendon, ligaments and blood vessels) and a consequential decline in the flexibility of joints, circulatory efficiency, skin integrity, etc., leads to impaired quality of life. The research proposed specifically aims to elucidate the cellular and molecular mechanisms of ECM tissue ageing. Three differing hypotheses ageing =(a) impaired turnover,(b) accumulated injury or(c) impaired cell signalling are tested in distinct and interlaced work packages underpinned by a cross-prograrrnne use of expertise in cell and molecular biology, biophysics and structural biology. The project aims to formulate anti-ageing strategies based on the fundamental knowledge gained.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain how 'Vesicular Golgi trafficking deficiencies in unsolved CDG type II patients' goes beyond current state-of-the-art under Seventh Framework.

assistant: **Innovation**

Glycobiology is considered as an emerging field of biology and the importance of complex carbohydrates in health and disease is increasingly recognized. In the last decade, a new group of diseases related to defects of glycosylation and called Congenital Disorders of Glycosylation has been identified. According to the cellular localization of the deficiency, CDG can be divided into two groups, CDG-I and CDG-II. Group I disorders relate to defects in the synthesis of the N-glycans in the endoplasmic reticulum. This project relates to Group II disorders, which arise from the defects in the processing of the glycans, mainly in the Golgi apparatus. Whereas all known CDG cases so far have defects in genes encoding proteins directly involved in the glycosylation process, our previous works highlighted that more cases may be linked to a defect of intracellular trafficking within the Golgi apparatus. This greatly expands the number of potential candidate genes and the proposed research aims at identifying new unsolved CDG-II patients with a trafficking deficiency in which localization and/or trafficking of Golgi associated glycosyltransferases appears disturbed. The first part of the project will be devoted to the screening of unsolved CDG-II patients for trafficking deficiencies and the second part will concern the identification of a new gene causing a new type of CDG with significant neuromuscular involvement. Indeed, we recently identified two patients in two different families with CDG and a very specific phenotype that includes myopathy, encephalopathy and epiphyseal hypoplasia. However and surprisingly, it appears that the patient cells also display a trafficking deficiency. We will aim to demonstrate the pathogenic nature of the unusual, homozygous mutation that we have
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Single-molecule visualization of transcription dynamics to understand regulatory mechanisms of transcriptional bursting and its effects on cellular fitness' (BURSTREG) targeting Horizon 2020.

assistant: **Abstract**

Transcription in single cells is a stochastic process that arises from the random collision of molecules, resulting in heterogeneity in gene expression in cell populations. This heterogeneity in gene expression influences cell fate decisions and disease progression. Interestingly, gene expression variability is not the same for every gene: noise can vary by several orders of magnitude across transcriptomes. The reason for this transcript-specific behavior is that genes are not transcribed in a continuous fashion, but can show transcriptional bursting, with periods of gene activity followed by periods of inactivity. The noisiness of a gene can be tuned by changing the duration and the rate of switching between periods of activity and inactivity. Even though transcriptional bursting is conserved from bacteria to yeast to human cells, the origin and regulators of bursting remain largely unknown. Here, I will use cutting-edge single-molecule RNA imaging techniques to directly observe and measure transcriptional bursting in living yeast cells. First, bursting properties will be quantified at different endogenous and mutated genes to evaluate the contribution of cis-regulatory promoter elements on bursting. Second, the role of trans-regulatory complexes will be characterized by dynamic depletion or gene-specific targeting of transcription regulatory proteins and observing changes in RNA synthesis in real-time. Third, I will develop a new technology to visualize the binding dynamics of single transcription factor molecules at the transcription site, so that the stability of upstream regulatory factors and the RNA output can directly be compared in the same cell. Finally, I will examine the phenotypic effect of different bursting patterns on organismal fitness. Overall, these a
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose the 'Concept and Approach' section for a Horizon 2020 proposal on 'When a Mafia Dies: the Criminogenic Effects of the Fight against Organised Crime'.

assistant: **Concept**

This project addresses the question of what happens when mafias are weakened, focussing on Japan and Italy. According to Diego Gambetta’s protection framework, which was expanded by Federico Varese, mafias replace the state in the everyday life of the local communities in which they operate.  Indeed, both the Italian mafias and the Japanese yakuza have exerted control over local communities, providing extra-legal protection and limiting the scope of ordinary criminality. Over the past fifty years, the struggle between mafias and the state has threatened democratic institutions and impacted the relationship between the state and society, prompting the state to introduce increasingly severe anti-organised crime (OC) legislation, which contributed to the weakening of these groups. While considerable efforts have been invested in this conflict, little research has addressed what happens when these policies are successful. The overall objective of the study is to identify the criminogenic effects resulting from the power vacuum left by a declining mafia, by: 1) analysing what determines the decline of a mafia in advanced liberal economies; 2) determining the socio-structural factors that favour the rise of new forms of criminality; 3) identifying the structure and modus operandi of the actors who take up the governing role in the markets previously monopolised by a mafia; 4) theorising an interdisciplinary approach to understand the long-term effects of the fight against OC; 5) suggesting anti-OC policies that empower civil society. JEOC will employ traditional archival, ethnographical, and visual research based on established research networks in Japan and Italy. The outputs will include a monograph on post-mafia governance and insurgent OCGs, a journal article on research
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe the specific objectives and expected outcomes for 'Roman Cements for Architectural Restoration to New High Standards' in the context of a Seventh Framework call.

assistant: **Objectives**

Highly hydraulic binders, known as natural or Roman cements, were key materials to cover façades of buildings of the European Historicism and Art Nouveau (19th/early 20th century), a period of rapid urban growth in Europe. The maintenance, restoration and reconstruction of historic Roman cement façades form therefore an important issue in Europe’s efforts to preserve its architectural heritage. Therefore, the ROCARE project is proposed to provide conditions for the industrial development and commercialisation of Roman cements which is an innovative, promising technology developed at the level of a pilot-scale prototype in the recent research project of the 5th Framework Programme ROCEM, 2003-2006. The increasing awareness of the conservation profession and their interest in the product call now for further actions encompassed by the proposed ROCARE-project, which aim at filling gaps in knowledge and reducing the entry barriers of the novel technology to the market. They include (a) scaling up of the RC technology to a competitive level by optimising the process technologies at various conditions of production, (b) laboratory tests and studies to fully understand cement hydration and property development, as well as  optimum conditions of mortar processing and handling in the conservation practice, and (c) broad dissemination measures to enlarge the market potential of the technology. The proposed project is designed for three-year duration and will be jointly conducted by 15 partners from industry, SMEs and research centres in 7 countries. It will allow the prototype developed in the earlier project to establish itself on the European market of building construction.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Sixth Framework proposal for the project 'Ecosystem Approach for Sustainable Aquaculture'.

assistant: **Objectives**

We propose an ecosystem approach to the aquaculture sector. We will address this by 1) identifying plausible quantitative indicators of the effects of aquaculture on ecosystems through a process of expert working groups, workshops and meetings, 2) similarly, identifying indicators of the main drivers of ecosystem change affecting aquaculture, including natural and environmental pressures, 3) assessing both sets of indicators using existing datasets (the partners collectively have extensive data archives), considering each in the context of appropriate selection criteria, 4) developing a range of tools, particularly models, that encapsulate best process understanding at a wide range of scales, 5) testing these models and indicators in a wide variety of field locations across Europe (~10) encompassing major culture species and technologies, and covering a wide spectrum of environment types, selected according to criteria developed during the project, and 6) using this data to test and select the final toolpack" of models and indicators, including appropriate decision support tools to guide users to effective implementation. National annual meetings with stakeholders will be held to allow 2-way interaction ensuring the practical relevance of the work and that the user community achieves ownership of the project's outputs. We will organise a final international conference and workshop where the "toolpack" of indicators and tools for effective environmental impact assessment and site selection will be demonstrated."
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Discovery of Bcl-3 inhibitors as Potential Chemopreventive Agent for skin cancer' (Bcl-Inhib).

assistant: **Concept**

Melanoma is one of the fastest growing cancers worldwide, especially in regions with a high number of Caucasians such as Europe. Currently, 100,000 diagnoses and 5,000 deaths from melanoma are registered in Europe every year. This increase in melanoma incidence is a serious health problem and poses an economic as well as societal concern. The survival in patients with malignant melanoma is very poor with a five year survival rate of about 10%. Indeed, the current clinical therapies against malignant and metastatic melanoma have a relatively low efficacy and are associated with significant adverse events. Therefore there is an urgent need for development of novel drugs that can cure or prolong the survival of these patients. Molecular targeted therapy by using a drug that reduces the growth and spread of cancer cells via inhibition of a specific protein is a foundation stone of precision medicine and treatment. We have identified a lead molecule that inhibits proliferation and metastasis of human malignant melanoma cells. The present proof-of-concept proposal aims at lead optimization, preclinical verification, IPR positioning, market assessment, and other commercial activities including go to market strategy and dialogue with potential collaborators. This will make a unique medical and commercial opportunity to develop a highly specific drug against a disease in urgent need of new treatment options.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Write a project proposal abstract for 'Frontal Impact and Compatibility Assessment Research' to be submitted under Seventh Framework.

assistant: **Abstract**

For the real life assessment of vehicle’s safety in frontal collision the compatibility (described by the self protection level and the structural interaction) between the opponents is crucial. Although compatibility has been analysed worldwide for years, no final assessment approach was defined. Taking into account the EEVC WG15 and the FP5 VC-COMPAT project activities, two test approaches are the most important candidates for the assessment of compatibility. Both are composed of an off-set and a full overlap test procedure. However, no final decision was taken. In addition another approach (tests with a moving deformable barrier) is getting more and more in the focus of today’s research programmes. Within this project different off-set, full overlap and MDB test procedures will be analysed to be able to propose a compatibility assessment approach, which will be accepted by a majority of the involved industry and research organisations. The development work will be acompanied by harmonisation activities to include research results from outside the consortium and to early disseminate the project results taking into account recent GRSP activities on ECE R94, EuroNCAP, etc..