RCaz/eu-funding-proposals-sft huggingface dataset:
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'FOSTERING EUROPEAN LAKES RESTORATION BY NUTRIENT REMOVAL, RECOVERY, AND REUSE: INTEGRATED CATCHMENT AND IN-LAKE SCALE APPROACH', explaining its relevance to EU policy priorities under Horizon Europe.

assistant: **Impact**

Eutrophication is a major problem causing poor ecological status of European lakes with its severe impacts being enhanced by climate change. However, at the same time, eutrophication could be the potential solution to the depletion of global phosphate (P) reserves, threatening global food security. A vast amount of P and other nutrients are lost from the catchment and transported into lakes, making most lakes a nutrient-rich reserve, as observed by the frequent occurrence of massive, devastating algal blooms in many lakes. FERRO bridges the nutrient enrichment problem to the depletion of P problem to create a sustainable solution to both challenges by circular management. We will develop a next-generation lake restoration approach by combining targeted restoration techniques with nutrient recovery and recycling to achieve multi-benefits: improved ecological status of lakes, support a circular economy, climate adaptation, support food production, promote biodiversity, and boost ecosystem services provision. The multiple environmental and socio-economic co-benefits extend beyond the scale of intervention, supporting wider sustainability and accounting for social and economic ambitions.FERRO supports the natural recovery of lakes (after many years of nutrient enrichment) through four transdisciplinary pillars: 1) classification and prioritization of lakes for restoration (integrated in-situ and remote sensing-based techniques); 2) implementation of sustainable catchment-oriented solutions (biotechnology to prevent nutrient losses in agriculture and nutrient recovery at lake inflows and reuse in agriculture), 3) implementation of sustainable in-lake restoration solutions (nutrient recovery from lakes and reuse in agriculture); and 4) knowledge transfer. FERRO marks a major
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Mathematical and Numerical Modelling of Process-Structure Interaction in Fractured Geothermal Systems' (MaPSI).

assistant: **Concept**

In the development and production of high-temperature geothermal resources, large induced gradients in pressure and temperature during injection operations results in complex dynamics. This includes boiling of geothermal fluids and deformation of the fractured rock, dynamics that are currently not well understood. This provides a core motivation as well as challenging test cases for the mathematical and numerical developments in the MaPSI project. MaPSI has as its main objective to provide mathematical models and simulation technology required to assess subsurface process-structure interaction in the context of hydraulic and thermal stimulation in development and production of high-temperature geothermal resources.This will be achieved by a highly novel and timely research programme which will: - Combine new techniques with recently developed methodology by my own research group to develop unprecedented mathematical and numerical approaches to enable modelling of multiphase flow and phase-change in thermo-poroelastic media with fractures that may slip, deform and propagate; - Advance competences in the main scientific fields involved; and - Improve understanding of coupled processes in development and production of high-temperature geothermal systems towards sustainable exploitation of the resource.The project will provide new mathematical and numerical models along with open-source software that have capabilities in modelling of coupled process-structure interaction in fractured porous media that greatly exceed those of existing platforms. Specific case-studies will assess process-structure interactions for development and production operations in high-temperature geothermal systems, including superheated and supercritical regimes, providing timely contributions to fur
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Insight into Drug Distribution and Local Concentration using Multispectral Imaging of Fluorescent Drugs in Inflammatory Bowel Diseases' demonstrating excellence, impact and implementation quality for a Horizon Europe call.

assistant: **Abstract**

Inflammatory bowel diseases (IBD) are chronic relapsing inflammatory disorders of the gastrointestinal tract affecting 2.5 Mil. patients in Europe alone. The majority of newly diagnosed patients are in adolescence or early adulthood and in the midst of their family life, career, and social development. Though not life-threatening, the disease comes with significant morbidity and complex treatment strategies and is associated with a high social burden and medical costs. msGUIDE aims to revolutionize IBD treatment by offering high-performance insight into local drug distribution and concentrations that can lead personalized medicine in IBD patients and early drug development through identifying specific targeted cells of drugs, elucidating the individual phenotype and thereby patient stratification. This will be achieved by reinvigorating endoscopy to overcome major challenges for medical application. The goal of msGUIDE is to develop a highly sensitive novel imaging technology consisting of a Near-Infrared Fluorescence and Reflectance Multispectral Imaging (NIR-FRMI) unit integrated into a never before seen High-definition White Light Endoscope (HD-WLE). The aim is to quantify drug distribution and concentration and identify the individual drug target cells in the gut. With this groundbreaking system we offer an operatorindependent endoscopic method to establish the optimal dose and to monitor the treatment response in IBD patients in realtime. This will allow for an individual, precise and quantitative diagnosis leading to a more personalized, targeted and thus more effective treatment in IBD and common diseases in general.


**Keywords:** Fluorescence endoscopy, multispectral imaging, novel tracers, personalized medicine
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Compose a compelling abstract for 'Mechanobiology of Aplysia neurons' demonstrating excellence, impact and implementation quality for a Seventh Framework call.

assistant: **Abstract**

Memory loss is a central symptom in different diseases such as Alzheimer’s disease, and represents a significant social and economic burden for a large percentage of European citizens. Neuronal cell adhesion molecules belonging to the immunoglobulin superfamily (IgCAMs) are known to be involved in brain development processes, and also contribute to the synaptic alterations connected with memory formation in adults. The goal of this project is to elucidate the molecular, biophysical and cellular mechanisms of directed movements of neuronal growth cones, and in particular how, upon binding to the extracellular matrix or to other cells, IgCAMs control cytoskeletal dynamics and therefore synaptic plasticity. The central hypothesis of this proposal is that adhesion-mediated growth cone guidance involves a force transduced by the cytoskeleton upon IgCAM adhesion, and that this mechanical signal further stimulates Src protein tyrosine kinase activation. In order to test this hypothesis, state-of-the-art techniques will be combined in a highly interdisciplinary manner. This project lies at the interface between mechanics, cell biology, biophysics and surface physics. The proposer will use a well-established cellular model system for growth cone studies (Aplysia), state-of-the-art molecular tools (recombinant IgCAM and Src biosensor) and a high-resolution force measurement system (Atomic Force Microscopy, AFM) coupled with FRET imaging. By applying the first molecule-specific AFM measurements to live neuronal growth cones, the proposer will measure the forces transduced by IgCAMs to the growth cone cytoskeleton and at correlating them with Src activity in real time, thereby proving the force-dependence of neuronal connectivity. This proposal is related to many of the FP7 researc
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft the 'Objectives and Scope' section of a Seventh Framework proposal for the project 'Multifunctional Photothermal Gold Nanoarrays for Cellular Manipulation'.

assistant: **Objectives**

Transporting, organizing, attaching, guiding, releasing entities of various dimensions over different scales remain central to the development of high throughput biological devices able to conduct millions of tests in a short period of time.  The proposed project intends to develop a new generation of substrates for cell manipulation based on photothermal processes. Specifically, it focuses on realizing standard and new functions related to tissue engineering and cell transportation by designing a single type of platform. This project aims to implement photothermal gold nanoparticle arrays (PGNAs) to physically manipulate various types of entities, like living cells. Each gold nanodot will play the role of an adhesive spot exposing the RGD peptide, which are separated by nonadhesive regions functionalized with poly(ethylene glycol) (PEG), so that cell-binding sites will exclusively interface the nanoparticle. By irradiating well-defined areas of the PGNAs with a focused laser beam, the fellow hopes to take advantage of the local heating generated by the nanoparticles to prevent cell attachment in these hot areas. This way, it is possible to study and integrate multiple functions in order to deliver one unique dynamic platform able to precisely control both cell adhesion (releasing, patterning, guiding) and cell transportation (via optofluidics) at the PGNA interface.  The “Multi-PGNAs” project may permit to integrate the same nanotechnology for the development of modern applications, while providing innovative methodologies for designing new experiments in cell biology and nanophysics.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an executive summary for the EU project 'Evolution of Prophages that carry Antibiotic Resistance Genes (ARGs) and their host-bacteria in response to antibiotics and increased lytic activity' (ARGs) targeting Horizon 2020.

assistant: **Abstract**

In the European Union, 25,000 deaths per year are caused by multidrug resistant bacteria. This trend is predicted to increase with ever increasing misuse and overuse of antibiotics, which accelerates the evolution of antibiotic resistance (AR). An important mechanism of transferring antibiotic resistance genes (ARGs) among bacteria are temperate bacteriophages (prophages), viruses that can incorporate their own genetic material into the bacterial chromosome, thereby providing their bacterial host (which is now called a ‘lysogen’) with additional genes, such as ARGs. Despite their vast abundance in nature, our understanding of the evolution of ARG-carrying prophages is still incomplete. Therefore I will study the evolution of ARG-carrying prophages and their host bacteria. Specifically, using constructed lysogens (carrying prophage lambda+ARG) of E. coli bacteria, I will (1) use competitive fitness assays to determine the costs/benefits for bacteria of carrying prophages that encode AR and how these costs depend on environmental antibiotic concentrations and the frequency with which the prophage enters the lytic cycle (i.e. the prophage becomes active, replicates and lyses the host cell). (2) I will follow the evolution of these lysogens that carry AR-encoding prophages using a serial transfer experiment in the presence/absence of antibiotics and compounds that induce phage lysis. (3) I will sequence the evolved lysogens including their prophage genomes to detect underlying genomic changes associated with bacterial adaptation to prophage carriage. I predict that the net effect of a prophage that encodes an ARG on the growth and evolution of its host bacterium will strongly depend on both the frequency with which the phage enters the lytic cycle and the costs/benefits of
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Participation, leadership, and urban sustainability' will create measurable scientific, societal and economic impact aligned with Fifth Framework.

assistant: **Impact**

Sustainable urban development is a key aim of policy makers at all levels of government. It is widely agreed that effective urban governance is of key importance in the achievement of sustainable city development. In order to realise effective urban governance it is crucial that city governments develop institutional forms and styles of leadership that promote decisiveness, facilitate the use of local knowledge and enhance public support. The objective of this proposal is to accumulate and disseminate practical knowledge about the complementarily of political leadership and citizen involvement in cities that are leaders in promoting sustainable development. By researching alternative approaches to urban leadership and community involvement in local decision-making in nine countries the project will identify aspects of good practice, and disseminate the research findings to cities, national governments, and the EU.
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Describe how 'Multidimensional Ultrafast Time-Interferometric Spectroscopy of Coherent Phenomena in all Environments' will create measurable scientific, societal and economic impact aligned with Seventh Framework.

assistant: **Impact**

We propose to develop and apply novel methods of nonlinear spectroscopy to investigate the significance and consequences of coherent effects for a variety of photophysical and photochemical molecular processes. We will use coherent two-dimensional (2D) spectroscopy as an ideal tool to study electronic coherences.Quantum mechanics as described by the Schrödinger equation is fully coherent: The phase of a wavefunction evolves deterministically in the time-dependent case. However, observations are restricted to reduced “systems” coupled to an “environment.” The resulting transition from coherent to incoherent behavior on an ultrafast timescale has many yet unexplored consequences, e.g. for transport in photosynthesis, photovoltaics or other molecular “nanomaterials.”In contrast to conventional 2D spectroscopy, we will not measure the coherently emitted field within a four-wave mixing process but rather implement a range of incoherent observables (ion mass spectra, fluorescence, and photoelectrons). Yet we can still extract all the desired information using “phase cycling” with collinear pulse sequences from a femtosecond pulse shaper. This opens up a new range of interdisciplinary experiments and will allow for the first time a direct nonlinear-spectroscopic comparison of molecular systems in all states of matter. Specifically, we will realize 2D spectroscopy in molecular beams, liquids, low-temperature solids, and on surfaces including heterogeneous and nanostructured samples. Tuning the external couplings will help elucidating the role of the environment in electronic (de)coherence phenomena.Furthermore, we will combine 2D spectroscopy with subdiffraction spatial resolution using photoemission electron microscopy (PEEM). This enables us to map transport in molecular aggr
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Draft an impact statement for 'ROLE OF ANTISENSE RNAs ON PU.1 REGULATION AND HEMATOPOIESIS', explaining its relevance to EU policy priorities under Seventh Framework.

assistant: **Impact**

PU.1 levels are critical for normal hematopoiesis and, even modest alterations can lead to leukemia and lymphoma. To achieve optimal expression of the PU.1 gene at different stages of hematopoiesis, multiple interactions are required between its regulatory elements to form fine chromatin architecture. While significant advances have been made in understanding which PU.1 locus chromatin states are active or silent, nothing is known how these states are propagated and maintained. The overall goal of the proposed project is to understand the role of antisense RNAs in the regulation of the PU.1 gene. Therefore I will (1) Characterize the timing, order, cellular localization, and levels of the natural noncoding antisense and coding PU.1 transcripts and formation of the active chromatin architecture within the PU.1 gene locus during cell development; (2) Investigate the functional effect of nuclear ablation of antisense concoding RNAs ablation on the formation of the active chromatin configuration and the expression of the PU.1 gene and, ultimately, on hematopoietic stem cell (HSC) fate; (3) Study the functional role of antisense noncoding RNAs in tissue culture and transgenic systems; and (4) Investigate the functional connection between the expression of the antisense noncoding RNAs and the occurrence of the other epigenetic marks, such as chromatin compaction, covalent histone modification, and DNA methylation. Accomplishment of these aims will enable us to understand the roles of antisense noncoding RNAs corresponding to PU.1 gene locus in the regulation of hematopoietic stem cell differentiation and to identify naturally occurring RNAmediated regulatory mechanisms, the manipulation of which might lead to critical changes in cell fate and provide knowledge of how changes
system: You are an expert grant writer specializing in European Union research funding proposals. You write compelling, well-structured proposal sections that demonstrate excellence, impact, and implementation quality, using precise EU terminology and following best practices for Horizon Europe, Horizon 2020, and earlier framework programmes.

user: Explain the overall concept, methodology and approach for the project 'Dynamics and stability of reed-dominated ecosystems in relation to major environmental factors that are subject to global and regional anthropogenically induced changes' (1994-1998).

assistant: **Concept**

Reeds are dying back at a fast rate in sizable areas of Europe, with significant impacts on important wetland functions (biodiversity, stability of river and lake margins, water quality) and local economies. EUREED II is a three-year strategic fundamental research initiative that aims at analyzing the mechanisms which control the growth dynamics and stability of reed-dominated ecosystems, at modelling and predicting how the ecosystem is disturbed by human activity and climate change, and at developing remedial management options. Expected achievements include (i)assessment of the functional role of reed-dominated ecotones as nutrient accumulators and transformers and as sources for atmospheric greenhouse gases, (ii) an ecosystem model capable of predicting future changes in ecosystem functioning in relation to climatic conditions, trophic status and water table management, (iii) assessment of genetic diversity of reed populations and its relation to die-back, and (iv) development of management tools, including preventative and restQrative measures in relation to die-back. The scientific approach of the workprogramme is multi-disciplinary and co-operative, and comprises observational field studies, field experiments, controlled greenhouse and laboratory experiments, data collation and treatment, and mathematical modelling. A significant part of the studies is carried out during multi-disciplinary workshops. The project is carried out by research groups with complementary skills and expertise from nine European countries. A reference site is selected in each country as the basis for the field studies. The reference sites cover boreal-mediterranean and oceanic-continental climatic gradients, and the observational and experimental studies at these sites permit interpolation