Flow cytometry software no relation mathematics
In cytometry, compensation is a mathematical correction of a signal overlap between the channels of the emission spectra of different fluorochromes.The photons emitted by fluorochromes have different energies and wavelengths and as flow cytometers use photomultiplier tubes (PMT) in order to convert the photons into electrons, the detector can register the signal from more than one fluorochrome. This creates a signal overlap (spillover) which cannot be removed by the optical system and has to be corrected electronically. The compensation can be done through different flow cytometry software such as Flowjo, Flowlogic, Kaluza etc. The first data compensation was done in 1977 by Michael Loken et al. during a two colour experiment, where mouse splenocytes were stained with fluorescein and rhodamine.
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