Harley Quinn performer Tara Strong
Animation
Harley Quinn has a cameo appearance in Justice League: The New Frontier during a speech by John F. Kennedy.
An alternate universe version of Harley Quinn appears in Justice League: Crisis on Two Earths. This version is a monkey of "The Jester" (the film version of the Joker).
Harley Quinn is featured in Batman: Assault on Arkham, voiced by Hynden Walch.
Harley Quinn appears in Lego DC Comics Super Heroes: Justice League: Gotham City Breakout with Tara Strong reprising her role.
Harley Quinn appears in Batman and Harley Quinn, voiced by Melissa Rauch. She works as a waitress after breaking up with the Joker again, and helps Batman and Nightwing stop Poison Ivy from accidentally causing the extinction of all life on Earth.
Dr. Harleen Quinzel appears in Batman vs. Two-Face, voiced by Sirena Irwin. She is the assistant to Hugo Strange, who reciprocates the Joker's flirting. In a Blu-ray exclusive bonus scene, Quinzel, dressed as Harley Quinn, busts the Joker out of prison.
Harley Quinn appears in DC Super Heroes vs. Eagle Talon, voiced by Kang Ji-young.
The Brave and the Bold version of Harley Quinn appears in Scooby-Doo! & Batman: The Brave and the Bold, with Tara Strong voicing her.
A feudal Japanese version of Harley Quinn appears in the anime film Batman Ninja, voiced by Rie Kugimiya and Tara Strong in Japanese and English respectively.
Harley Quinn appears in Teen Titans Go! To the Movies. She has a cameo appearance in an altered future where the villains have taken over.
Harley Quinn appears in Batman vs. Teenage Mutant Ninja Turtles, with Tara Strong reprising her role. Harley is first introduced as an inmate at Arkham, who mocks Shredder from inside her cell. After the Joker frees her, she is mutated into a crazy and anthropomorphic hyena and assists her lover in fighting the Bat family and the Turtles. She is knocked out by a crazed and mutated Batman, and abandoned by the Joker as he attempts to escape. She was presumably permutated by the Gotham police.
Dr. Harleen Quinzel makes a non-speaking cameo appearance in Batman: Death in the Family.
Harley Quinn has a cameo appearance in Space Jam: A New Legacy on a train that LeBron James and Bugs Bunny are on while searching the DC World in the "Serververse" for the Toons.
Harley Quinn appears in the animated film Injustice, voiced by Gillian Jacobs.
Harley Quinn appears in Teen Titans Go! & DC Super Hero Girls: Mayhem in the Multiverse, with Tara Strong reprising her role. Following the events leading up to the second season of the series, Harley joins the Legion of Doom, created by Lex Luthor to get rid of all of the superheroes and take over the world using the Amulet of Cythonna. However, she disagreed with Luthor and the Legion's plan and left the villains after helping Wonder Woman escape, now that she knew her friend's identity as Batgirl. Inspired by Bumblebee's speech, she decides to help the heroes defeat Cythonna and the Legion of Doom. After their success, the Super Hero Girls end up accepting Harleen into their team.