Glycine photosynthesis via C−N coupling of waste plastic and nitrate over diatomic Pd−B catalyst

Domain: chemistry
Published: 2026-01-20
Editorial outcome: minor_revision

Abstract
Photocatalytic C−N coupling offers a promising approach for the sustainable production of amino acids, but the uncontrolled coupling pathway of reaction intermediates limits yield and selectivity. This paper describes a Pd−B diatomic catalyst that can selectively catalyze the photosynthesis of glycine via C−N coupling reaction between waste poly(ethylene terephthalate)-derived ethylene glycol and nitrate, achieving a glycine yield of 2.9 mmol g−1 cat h−1 with a selectivity of 92%. Mechanistic investigations reveal that ethylene glycol is photo-oxidized to glycolaldehyde at the hole-rich B site, while nitrate is photo-reduced to NH4+/NH3 at the electron-rich Pd site. Subsequently, glycolaldehyde undergoes C−N coupling with NH4+/NH3, and further photo-oxidized to form glycine. The Pd−B diatomic site more effectively stabilizes the glycolaldehyde intermediate, resulting in a more favorable C−N coupling pathway than metal oxide and enhancing glycine selectivity. Thus, we show a catalytic system for selective glycine photosynthesis by precisely regulating the reaction pathways of key intermediates.

Introduction
Glycine, the simplest α-amino acid in structure, serves not only as a fundamental unit in protein synthesis but also as an indispensable key material across diverse fields, including food, pharmaceutical, agricultural, and organic synthesis1–4. Currently, the global annual production of glycine has surpassed 740,000 tons5, and the demand is exhibiting a steady growth trend with the continuous expansion of the pesticide, pharmaceutical and food industries6. However, conventional glycine synthesis methods, particularly the widely employed Strecker approach, are hindered by several significant limitations, such as the reliance on highly toxic cyanide-based reagents, harsh reaction conditions, and intricate synthetic pathways. These factors significantly impact the quality of glycine produced, as well as its production costs and environmental sustainability (Fig. 1a)7–9. Consequently, developing mild, efficient and sustainable pathways for glycine production holds substantial strategic importance for the rational utilization of energy resources and environmental protection.

The photocatalytic conversion of carbon-containing small molecules (such as CO2, CH4, or methanol) and nitrogen-containing substrates (such as N2, or NH3) into amides or amino acids via the C−N coupling reaction has emerged as a highly promising sustainable strategy because of its mild reaction and high atom economy10–17. In particular, polyols, acting as highly active photochemical reagents, have been established as renewable carbon precursors for amino acid synthesis. Specifically, the hydroxyl group (C−OH) in polyols can be photo-oxidized to generate electrophilic aldehyde groups (H−C=O)13,18, which then react with nitrogen-containing nucleophiles to construct C−N bonds13,18,19. Compared with inert N2 (946 kJ/mol), nitrate (NO3−) in wastewaters serves as a more suitable nitrogen source for photocatalytic C−N bond formation due to its high polarity and low N=O bond dissociation energy (204 kJ/mol)20,21. To date, only a few rare active catalysts (TiO2, CdS) have been reported for the photocatalytic synthesis of glycine via C−N coupling reaction from CH4, CO2, or methanol2,10,11,14. Nevertheless, despite these advances, the selectivity and yield of glycine remain unsatisfactory, primarily due to side reactions involving high-energy C−C coupling and the inevitable overoxidation process of key intermediates (electrophilic aldehydes) into organic acids or CO2 terminal byproducts (Fig. 1b)22,23. These bottlenecks underscore the significance and challenge of designing a photocatalytic system capable of achieving selective C−N coupling to attain high yields of glycine.

Poly(ethylene terephthalate) (PET), as the most produced and widely consumed polyester plastic globally, boasts an annual output of approximately 100 million tons, primarily synthesized through the polymerization of terephthalic acid (TPA) and ethylene glycol (EG)24. However, over 80% of PET waste ends up in landfills or is discharged into the environment, posing a severe threat to ecosystems and resulting in significant carbon resource waste25,26. Chemical hydrolysis of PET into TPA and EG monomers is generally regarded as a highly promising recycling approach (Fig. 1c)27,28. Nevertheless, in practical implementation, the separation and purification of EG via distillation are operationally complex and costly due to its high boiling point (197.3°C) and water solubility29. Moreover, given the relatively low market price of EG ($800 per ton) and its widespread availability, it is even less cost-effective to purify the hydrolyzed EG solution for reuse in PET production. Instead, photocatalytic oxidation of PET-derived EG into higher-value-added products, such as glycolic acid (GA, $20000 per ton) or acetic acid (AA, $3000 per ton), has emerged as a highly attractive alternative pathway (Fig. 1c)30–33. However, the significant challenge of this photocatalytic process is low selectivity and a limited product scope, primarily acids. Developing a strategy to expand the product range will help to reintroduce waste carbon from plastics into the utilization cycle34. Sufficient evidence has demonstrated that the electrophilic glycolaldehyde serves as a key intermediate in the photooxidation of EG to form organic acids32,35,36. Therefore, developing a catalyst capable of effectively stabilizing and capturing electrophilic glycolaldehyde intermediates during EG photo-oxidation to achieve selective C−N coupling with nitrate for photosynthesis of high-value glycine ($4600 per ton) would be a highly promising and scientifically valuable research strategy.

Atomically dispersed catalysts, owing to their tuneable coordination environments and electronic structure, offer the possibility for the selective adsorption and activation of substrates or reaction intermediates37,38. In particular, diatomic catalysts featuring dual redox active sites demonstrate distinct advantages over single-atom catalysts in simultaneously enhancing photocatalytic redox activity and product selectivity36,39–42. Therefore, we hypothesize that constructing diatomic photocatalysts containing redox sites could simultaneously strengthen the selective adsorption and activation of EG and NO3−, thereby achieving both high efficiency and selectivity in the photocatalytic synthesis of glycine. To verify the above hypothesis, we develop an efficient glycine photosynthesis route via C−N coupling of waste PET-derived EG and NO3− over a Pd−B diatomic catalyst (Fig. 1d). Mechanistic analysis shows that the key intermediates in glycine photosynthesis include an electrophilic glycolaldehyde generated via the photocatalytic oxidation of EG at the hole-rich B sites, and NH4+/NH3 produced through the photocatalytic reduction of NO3− at the electron-rich Pd sites. Subsequently, the C−N coupling between glycolaldehyde and NH4+/NH3 yields ethanolamine, which is further photo-oxidized to glycine. In this process, the Pd−B diatomic site is more conducive to stabilizing the glycolaldehyde intermediate to complete the C−N coupling pathway and avoid deep oxidation to organic acids compared to metal oxide catalysts, resulting in a high selectivity for glycine (>92%). Furthermore, the strong electronic interaction between the Pd−B diatomic sites enhances light absorption and photoelectron transfer dynamics, thereby facilitating the efficient photosynthesis of glycine, with a generation rate of 2.9 mmol g−1 cat h−1. This process has been successfully scaled up to the gram level, enabling the conversion of real-world PET plastic waste and biomass-derived polyols into glycine using natural sunlight.

Results
Catalyst synthesis and characterization
B-doped carbon nitride-anchored Pd single-atom catalyst (Pd SA/BCNx) was synthesized according to our previously reported procedure with minor modifications (see methods)36. Transmission electron microscopy (TEM) images reveal that the Pd SA/BCNx catalyst possesses a hollow nanotube morphology with an average diameter of 550±20 nm and an average wall thickness of 110±5 nm (Fig. 2a). High-resolution transmission electron microscopy (HR-TEM) image confirms the absence of discernible metal or metal oxide nanoparticles within the catalyst (Fig. 2b). Aberration-corrected high-angle annular dark field-scanning transmission electron microscopy (AC-HAADF-STEM) shows numerous bright spots scattered on the nanotube (Fig. 2c), providing direct evidence for the atomically dispersed state of palladium on the support. Furthermore, energy-dispersive X-ray spectroscopy (EDS) elemental mapping images clearly reveal the uniform distributions of B, C, N, and Pd on the carbon nitride nanotubes (Fig. 2d).

The crystal phase and molecular structure of the catalysts were further identified through X-ray diffraction (XRD) patterns, Fourier transform infrared (FT-IR) spectroscopy and electron spin resonance (ESR) spectroscopy. The results indicate that the BCNx support retains the crystalline phase43,44 and molecular structure of graphitic carbon nitride (g-C3N4), and successfully incorporates cyano groups (C≡N) into the molecular structure (Supplementary Fig. 1a–c)45. Furthermore, the solid-state 11B NMR spectrum of Pd SA/BCNx precisely reveals that B atoms partially substituted the C-terminal positions of the triazine units and resided at the B1 site in Pd SA/BCNx (Supplementary Fig. 1d). The corresponding molecular structure is shown in the inset of Supplementary Fig. 1d, and the yellow ball indicated by the arrow represents the B1 position in Pd SA/BCNx. X-ray photoelectron spectroscopy (XPS) further confirms the chemical and oxidation states of C, N, B and Pd elements in Pd SA/BCNx, and the doping content of B and Pd is approximately 1.37 wt% and 1.04 wt%, respectively (Supplementary Fig. 2a–d and Supplementary Table 1). The doping content of B and Pd is also further determined through EDS elemental analysis (B: 1.24 wt%; Pd: 0.98 wt%) and inductively coupled plasma optical emission spectrometry (ICP-OES) (Pd: 1.07 wt%). The B 1s spectrum of the BCNx sample exhibits a prominent peak at 191.9 eV, attributable to the B−N bond (Supplementary Fig. 2c)36,46. The Pd 3d spectrum shows two peaks at 342.9 eV and 337.6 eV in the B-free Pd SA/CN sample, corresponding to Pdδ+ 3d3/2 and Pdδ+ 3d5/2 (0<δ<2), respectively (Supplementary Fig. 2d)47,48. Notably, compared with BCNx, the B−N bond peak in Pd SA/BCNx shifts to a higher binding energy by 0.2 eV, whereas the Pdδ+ peaks shift to a lower binding energy by 0.2 eV, suggesting a stronger electronic interaction between the atomic Pd site and the B site.

The local coordination environment and oxidation state of Pd single atoms in Pd SA/BCNx were further determined by X-ray absorption spectroscopy (XAS). The Pd K-edge X-ray absorption near-edge structure (XANES) spectra reveal that the absorption edges for Pd SA/BCNx lie between those of the Pd foil and PdO references (Fig. 2e), indicating an oxidation state of Pd between 0 and +2. Fourier transformed extended X-ray absorption fine structure (EXAFS) spectra exhibit a dominant feature at 1.50 Å, revealing the presence of Pd−N coordination in Pd SA/BCNx (Fig. 2f)48,49. No Pd–Pd coordination peaks are detected at 2.96 Å and 2.45 Å, confirming that the Pd species in Pd SA/BCNx are atomically dispersed without metallic Pd or PdO clusters. The EXAFS fitting results suggest that the Pd single-atom site in Pd SA/BCNx is close to a Pd–N coordination site (Fig. 2g, Supplementary Fig. 3 and Supplementary Table 2). The Pd−N coordination is also visually reflected from wavelet transform (WT) EXAFS analysis of Pd SA/BCNx (Fig. 2h–j), showing a single intensity maximum only in the lobes centered at 6.30 Å−1 in k-space, corresponding to the N atoms around Pd. The molecular structure model of Pd SA/BCNx is determined from the XPS and solid-state 11B NMR results (inset of Fig. 2g).

Photocatalytic C−N coupling for glycine production
Photocatalytic C−N coupling reaction for the glycine production was attempted in an aqueous solution of EG and NO3− (Fig. 3a). The reaction comprises two fundamental steps of EG photooxidation and NO3− photoreduction. The EG solution (0.1 M) in the reaction was prepared via hydrolysis of PET plastic (Methods, Supplementary Fig. 4). First, the 1H NMR spectra of commercial GA, EG and glycine were applied to determine the characteristic peaks of the substrate (EG, 3.52 ppm), the target product (glycine, 3.40 ppm), and the byproduct (GA, 4.10 ppm) (Supplementary Fig. 5). Some metal oxides, metalsulfides, and carbon nitride (g-C3N4), have been demonstrated to effectively catalyze the conversion of EG into aldehyde-containing intermediates, thereby meeting the necessary conditions for photocatalytic C−N coupling10,11,14,32. Subsequently, a series of photocatalysts was screened, including metallic oxides (ZnO, TiO2, WO3), metallic sulfides (CdS, MoS2) and g-C3N4 (B-free carbon nitride, CN; B atom-doped carbon nitride, BCNx), for glycine photosynthesis from EG and NO3− (Fig. 3b and Supplementary Fig. 6a). Metal oxide catalysts inevitably lead to the formation of formic acid (FA), whereas metal sulfide- and g-C3N4-based catalysts do not exhibit this phenomenon, primarily due to the overoxidation of EG occurring on metal oxides. Based on these preliminary screening results, the BCNx demonstrates the optimal catalytic performance, achieving a glycine selectivity close to 100% and a reaction activity of 0.82 mmol g−1 cat h−1. The introduction of metal single-atoms onto BCNx support can generate strong electronic interactions, which are expected to optimize the light absorption, charge transfer and separation capabilities39, as well as enhance the catalytic activity for C−N coupling reactions. Specifically, different metal single-atoms (Pt, Pd, Au, Ag, Cu, Co and Fe) were anchored on the BCNx support to construct dual active site catalysts to increase glycine production activity (Fig. 3c and Supplementary Fig. 6b). The catalytic performance reveals that the Cu SA/BCNx cannot significantly improve the catalytic activity, and the Pt/Fe SA/BCNx reduce the selectivity towards glycine due to the formation of more AA by-products. In contrast, Pd/Au/Ag/Co SA/BCNx catalysts achieve increased rates of glycine generation and maintain high selectivity with a small amount of GA product. Notably, the Pd SA/BCNx achieves the highest glycine generation rate of 2.9 mmol g−1 cat h−1 with a selectivity of 92%, and this performance is competitive (Supplementary Table 3). Moreover, the apparent quantum efficiency (AQE) can reach 0.39% under monochromatic irradiation at 365 nm (Supplementary Fig. 7).

A series of control experiments were conducted to further investigate the catalytic mechanism and identify key factors influencing the reaction. No glycine was detected under conditions lacking light, catalyst, EG or NO3− (Fig. 3d), indicating that the reaction is a photocatalytic C−N coupling process driven by EG and NO3− in the presence of the Pd SA/BCNx catalyst. The reaction conditions were further optimized as 1% Pd loading on BCNx and 0.1 M EG/0.5 M KNO3 (Fig. 3e), indicating that the Pd and NO3− concentrations play crucial roles in the catalytic activity. Furthermore, when a mixture of anhydrous EG and KNO3 was subjected to photocatalytic C−N coupling, no glycine product was observed (Supplementary Fig. 8), revealing that water is indispensable in this system. Subsequently, the synergistic effect of Pd and B sites in photocatalytic C−N coupling for glycine production was investigated. All three catalysts (Pd SA/CN, BCNx, Pd SA/BCNx) exhibit glycine selectivity exceeding 92% (Fig. 3f), suggesting that the high selectivity does not primarily rely on the modulation of electronic structure and optical properties of the support by Pd doping or B sites. Compared to metal oxides, the g-C3N4-based support plays a key role in achieving highly selective photosynthesis of glycine (Fig. 3b). The Pd SA/BCNx demonstrates the highest glycine production activity of 69.4 mmol g−1 cat, approximately 3.5 times that of the BCNx (19.6 mmol g−1 cat) and 2.3 times that of the B-free Pd SA/CN catalyst (29.7 mmol g−1 cat) (Fig. 3f), indicating that stronger interactions between the Pd−B diatomic sites can synergistically enhance the catalytic activity. The conversion efficiency of EG and NO3− on the Pd SA/BCNx catalyst are 23.4% and 5.6%, respectively, within 24 h (Supplementary Fig. 9), and the total molar amount of detected products (glycine and GA) was approximately equal to the amount of consumed EG. In addition, the glycine production activity of the Pd nanoparticle-loaded catalysts (Pd NP/BCNx, 40.9 mmol g−1 cat) was comparatively lower than that of the Pd SA/BCNx (Supplementary Fig. 10), which may be attributed to the fact that atomic Pd can expose more active sites, thereby enhancing the selective adsorption and activation of key intermediates to facilitate the C−N coupling process.

In addition to catalytic activity, long-term stability is also a crucial factor for practical applications. The Pd SA/BCNx catalyst exhibits recycling stability, with no significant decrease in glycine production observed after five cycles (Fig. 3g). The XRD, FT-IR, and TEM images reveal that the crystal structure, chemical composition, and microstructure of the Pd SA/BCNx remain unchanged after the reaction (Supplementary Fig. 11). Quantitative analysis of the 1H NMR spectra (Fig. 3h) reveals that the yield of glycine increased linearly with increasing time while maintaining high selectivity (>92%) (Fig. 3i). Notably, the selective production of glycine alone is achieved by precisely controlling the reaction time within 24 h, which significantly simplifies the subsequent separation and purification steps for the target product (Fig. 3j). The target product was isolated and characterized using gas chromatography−mass spectrometry (GC‒MS). The characteristic peak observed at m/z=75 in the mass spectrum matched that of the commercial glycine standard (Fig. 3k), indicating the promising application of the dual-site Pd−B catalyst for efficient glycine photosynthesis.

Photocatalytic mechanism
A series of photoelectric characterizations were performed to elucidate the underlying mechanism of the photocatalytic glycine generation. Initially, the light absorption capabilities of the samples were evaluated via UV‒vis diffuse reflectance spectroscopy. Compared with pure CN, both BCNx and Pd SA/BCNx exhibit significantly improved UV‒visible light absorption (Fig. 4a), which can be attributed to the local electron reconstruction induced by the incorporation of Pd−B diatomic sites and C≡N groups50. Based on Tauc plot analysis, the bandgap energies are calculated to be 2.45 eV (CN) and 2.20 eV (BCNx, Pd SA/BCNx) (Supplementary Fig. 12a). The XPS valence band spectra reveal valence band maximum positions of CN, BCNx and Pd SA/BCNx at 1.62 eV, 1.52 eV and 1.46 eV, respectively (Supplementary Fig. 12b). The result indicate that the band structure (Supplementary Fig. 12c) fulfills the redox potential of EG oxidation to glycolaldehyde (0.55 eV)32 and NO3− reduction to NH4+/NH3 (−0.58 eV)51,52. Furthermore, the separation efficiency of photoinduced carriers was investigated through integrated photoluminescence (PL) and time-resolved PL (TR-PL) measurements. The PL spectra reveal that the emission intensity of Pd SA/BCNx is significantly quenched (Supplementary Fig. 13a), which can be attributed to the rapid electron transfer from the B atom site to the Pd atom site. Moreover, the TR‒PL spectra show that the average emission lifetime of Pd SA/BCNx (τave = 2.15 ns) is markedly shorter than that of BCNx (τave = 2.36 ns) and CN (τave = 15.49 ns) (Supplementary Fig. 13b and Supplementary Table 4), confirming that the strong electronic interactions between the Pd−B diatomic sites effectively suppress the recombination of charge carriers. The charge separation behavior of Pd SA/BCNx was also further confirmed through transient photocurrent response (TPR) and electrochemical impedance spectroscopy (EIS) measurements. Compared with BCNx and CN, the Pd SA/BCNx exhibits a greater photocurrent response (Supplementary Fig. 13c) and a smaller semicircle diameter in the EIS Nyquist plots (Supplementary Fig. 13d), indicating its enhanced efficiency in electron-hole pair separation and transfer.

In situ XPS was conducted under dark and illuminated conditions to explore the enhanced electronic interaction and photoelectron transfer dynamics between the atomic Pd site and the B site in Pd SA/BCNx during the photocatalytic process. Under illumination, the binding energy peak of the B−N bond shifts to a higher binding energy by 0.2 eV, whereas the peaks of Pdδ+ shift to a lower binding energy by 0.2 eV (Fig. 4b, c). Both peaks revert to their original positions upon cessation of illumination, indicating that electrons can rapidly transfer from the B site to the atomic Pd site upon illumination. This dynamic electronic modulation is crucial for optimizing the redox reaction sites during glycine synthesis pathways. Furthermore, density functional theory (DFT) calculations reveal that the introduction of B confirms the strong electron interactions between the atomic Pd site and the B site (Supplementary Fig. 14). Femtosecond transient absorption spectroscopy (fs-TAS) was employed to investigate the photoelectron transfer kinetics of Pd SA/BCNx and BCNx (Fig. 4d–g). Upon excitation with a 400 nm pump pulse, both Pd SA/BCNx and BCNx are excited from the ground state to the excited state, with the probe wavelength ranging from 410 nm to 750 nm. The TAS of BCNx exhibits a distinct negative peak at ~440 nm (Fig. 4e, g), corresponding to ground-state bleaching, indicating the relaxation process of the excited state. In contrast, the signal intensity at 440 nm for Pd SA/BCNx is significantly weaker than that of BCNx, and an additional negative peak at ~560 nm is observed at early delay times (Fig. 4d, f). The kinetic analysis of the absorption decay curve reveals the average ground-state bleaching (GSB) recovery times of BCNx (314.88 ps) and Pd SA/BCNx (264.12 ps), respectively (Fig. 4h and Supplementary Table 5), suggesting efficient the photoelectron transfer from the BCNx to the Pd atom (Fig. 4i)53. These results highlight the pivotal role of the Pd−B diatomic sites in enhancing light absorption, optimizing band structure, and accelerating the photoelectron transfer dynamics.

An in-depth mechanistic study was conducted to explore the reaction mechanisms of photocatalytic C−N coupling for glycine production. First, the role of photogenerated electron‒hole pairs in glycine photosynthesis was investigated by introducing different scavengers (Fig. 5a). The addition of 0.1 M nitrobenzene as an electron scavenger significantly reduces the glycine yield from 69.4 to 11.6 mmol g−1 cat, indicating that photogenerated electrons are crucial for NO3− reduction. Moreover, the introduction of 0.1 M phenol as a hole scavenger into the reaction leads to a decrease in the glycine production to 3.9 mmol g−1 cat, revealing that photogenerated holes predominantly drive the oxidation of EG. Moreover, when 0.1 M 5,5-dimethyl-1-pyrroline N-oxide (DMPO, a sacrificial radical agent) was added, the glycine yield also decreases substantially to 19.8 mmol g−1 cat, suggesting that the formation of glycine may also be driven by photogenerated radicals.

Furthermore, in situ electron spin resonance spectroscopy (ESR) was performed using a hole-trapping agent (2,2,6,6-tetramethylpiperidine-1-oxyl, TEMPO) and the radical trapping agent DMPO. Under light irradiation, the signal peak of TEMPO-h+ significantly weakens in pure H2O (Supplementary Fig. 15), suggesting that light can induce the generation of numerous photogenerated holes in Pd SA/BCNx that participate in oxidation reactions. Quantitative ESR analysis was conducted for Pd SA/CN and Pd SA/BCNx under the same TEMPO concentration (Fig. 5b). The TEMPO signal peaks are the lowest in the Pd SA/BCNx system under illumination, demonstrating that the incorporation of B-atom sites promotes the rapid accumulation of photogenerated holes. When H2O oxidation matches the oxidation reaction, the signals of DMPO-OH are recorded, corresponding to hydroxyl radicals (•OH) produced by the oxidation of water by photogenerated holes (Fig. 5c). Notably, upon introducing EG into the reaction system, a typical sextet signal characteristic of an alkoxy radical (•CHOHCH2OH, AN = 15.9 G, AH = 22.7 G) appeared in the ESR spectrum (Fig. 5d and Supplementary Fig. 16)10,54. However, the signal peak of DMPO-OH completely disappears, indicating that the oxidation of EG takes precedence over H2O oxidation by photogenerated holes to produce •CHOHCH2OH. When both EG and NO3− are present simultaneously, the •CHOHCH2OH signal peak further intensifies, suggesting that NO3− is preferentially reduced over water to consume more photogenerated electrons, further demonstrating that the generation of •CHOHCH2OH is directly related to the increase in glycine yield. In addition, no •O2− or H2O2 is produced in the pure H2O, EG and EG/NO3− systems, indicating that the oxidation reaction is only driven by the photogenerated holes (Supplementary Fig. 17).

Subsequently, comparing the gas-phase products in the three systems (Supplementary Fig. 18), it is found in the pure H2O system, Pd SA/BCNx is more inclined to undergo photocatalytic overall H2O splitting, producing trace amounts of H2 and O2. In the EG aqueous solution system, the EG molecules more readily consume the holes, causing Pd SA/BCNx to generate more photogenerated electrons for reducing H2O to H2. However, in the EG/NO3− system, no gaseous products are detected, suggesting that the photogenerated electrons are exclusively utilized for the reduction of NO3−. The above results indicate that EG molecules can be effectively activated to form •CHOHCH2OH through dehydrogenation, thereby generating an H-C=O precursor for the subsequent C−N coupling40. Therefore, photogenerated holes predominantly drive the oxidation of EG to generate alkyl radicals, whereas photogenerated electrons mainly participate in the reduction of NO3− to form nitrogen intermediates (NO2−, NH3, or NH4+). The generation of alkyl radicals and nitrogen intermediates creates favorable conditions for the subsequent C−N coupling reaction.

After identifying the key factors driving the enhanced efficiency of glycine synthesis by the Pd SA/BCNx catalyst, in situ attenuated total reflection Fourier transform infrared spectroscopy (ATR−FTIR) was employed to investigate the key intermediates in the glycine photosynthesis. The in situ ATR−FTIR spectra clearly reveal information on intermediates formed in two pathways of EG oxidation (yellow label) and NO3− reduction (purple label) (Fig. 5e, f). For the NO3− reduction pathway, characteristic stretching vibration bands of NO3− (1280 cm−1), NH3 (1120 cm−1) and NH4+ (1400 cm−1) are detected12,55,56, and their intensities increase progressively with reaction time. Furthermore, UV‒vis spectroscopy analysis confirms the presence of absorption peaks at 540 nm and 651 nm, corresponding to the characteristic absorptions of complexes formed by NO2− and NH4+ ions, respectively (Fig. 5g, h)21. Thus, NH4+ and NH3 are identified as the main products of nitrate reduction, and NO2− is regarded as the primary intermediate. For the EG oxidation pathway, a characteristic C=O stretching vibration peak of the key intermediate glycolaldehyde was observed at 1760 cm−1. The C−OH vibrational band at 1030 cm−1 could be attributed to glycolaldehyde, ethanolamine (EOA) or glycine57. Notably, a distinct C−N vibrational band at 1479 cm−1 belongs to glycine or EOA5, and its intensity increased steadily throughout the reaction. Therefore, glycolaldehyde, EOA and NH4+/NH3 are likely to be the critical intermediates for the C−N coupling reaction in glycine photosynthesis.

A series of designed control experiments were conducted to further identify the critical intermediates involved in the C−N coupling reaction. When EG and different ammonium salts (NH4Cl, (NH4)2SO4 and (NH4)2CO3) were used as reactants, the glycine yield was significantly increased to 105.5 mmol g−1 cat, 80.8 mmol g−1 cat and 90.6 mmol g−1 cat, respectively, compared with that of the EG/KNO3 system (69.4 mmol g−1 cat) (Fig. 5i and Supplementary Figs. 19, 20). In addition, trace amounts of glycine (6.0 mmol g−1 cat) can still be found in the EG/NH3•H2O system. In contrast, the glycine yield is undetectable in the EG/NH2OH system, indicating that NH4+ and NH3 are the key intermediates for the C−N coupling process during the photoreduction half-reaction (NO3− to NH4+/NH3). Moreover, in the conventional oxidation pathway of EG (Fig. 5k, Pathway 1)32, glycolaldehyde is generated via the oxidative dehydrogenation of EG and can be further oxidized to FA byproducts.

A series of control experiments were also performed to identify the key intermediates at the oxidation end. When GA is used as a substrate to react with NO3−, NH4+, NH3 and NH2OH, only trace amounts of glycine can be detected in the GA/NH4+ system, whereas the formation of FA can be observed in the NO3−, NH4+ and NH3 systems. When glyoxalic acid was used as the substrate instead of GA, the FA yields in all three systems further increased without any production of glycine. Therefore, glycolaldehyde from the oxidation and dehydrogenation of EG serves as the key intermediate for the C−N coupling process in the photooxidation half-reaction (EG to glycolaldehyde). Similar results were also observed with the pure CN catalyst, although the glycine yield was lower than that of Pd SA/BCNx (Supplementary Fig. 21), suggesting that the g-C3N4-based support follows the same reaction pathway, and the introduction of Pd−B diatomic sites significantly enhances the C−N coupling reaction.

After identifying the key intermediates, the subsequent reaction pathways were explored. It was hypothesized that glycolaldehyde would then undergo a preliminary C−N coupling reaction between the aldehyde groups and NH4+/NH3 to generate the EOA intermediate. Subsequently, EOA is proposed to be further oxidized to glycine. To validate this hypothesis, the Pd SA/BCNx was subjected to a photocatalytic reaction in a 0.02 M EOA solution. The 1H NMR spectra reveal a decrease in the characteristic peaks of EOA over time, accompanied by the appearance of peaks corresponding to glycine (Fig. 5j), thereby confirming the oxidation of EOA to glycine catalyzed by the Pd SA/BCNx, consistent with the reported results58,59. A comparison of glycine yields for EOA oxidation was performed over CN, BCNx, Pd SA/CN and Pd SA/BCNx, and the results reveal that the glycine yields are significantly higher for B-containing catalysts than B-free catalysts, with the yield achieved by BCNx even surpassing that of Pd SA/CN. This observation further substantiated that the oxidation of EOA primarily occurs at the hole-rich B site (Supplementary Fig. 22). Therefore, it was inferred that the key intermediate glycolaldehyde may undergo two pathways during the reaction. The pathway 1 involves further oxidation of glycolaldehyde to form GA, and the pathway 2 involves the amination of glycolaldehyde to form EOA (Fig. 5k and Supplementary Fig. 23a). The high glycine selectivity exhibited by the Pd SA/BCNx may be attributed to its enhanced capability to facilitate the amination of glycolaldehyde, facilitating the timely formation of EOA (Fig. 3b, c). In addition to the spectroscopic evidence mentioned above, DFT calculations were employed to investigate the reaction energies for two pathways over TiO2 and Pd SA/BCNx. The conversion of glycolaldehyde to GA was found to possess a larger negative reaction energy on TiO2 than Pd SA/BCNx, indicating that TiO2 is more favorable for the oxidation of EG to GA (Pathway 1), which is further oxidized to produce FA by-products (Supplementary Fig. 23b). In contrast, the Pd SA/BCNx surface is more inclined to promote the amination of glycolaldehyde to form EOA (Supplementary Fig. 23c), which is further oxidized to glycine (Pathway 2). These results provide a rational theoretical explanation for the high selectivity of Pd SA/BCNx for glycine production. However, due to the inherent simplification of DFT, there are still limitations in accurately capturing the real catalytic process when simulating catalytic conditions.

Based on the aforementioned experimental results, a possible reaction mechanism is proposed to elucidate the photosynthesis of glycine from EG and NO3− (Fig. 5k, Pathway 2). First, under illumination, electrons in the valence band of the Pd SA/BCNx catalyst are excited to the conduction band, resulting in photogenerated electron and hole pairs. The photogenerated holes oxidize EG to glycolaldehyde via a dehydrogenation process. The photogenerated electrons facilitate the reduction of NO3− to NH4+/NH3. The basic nitrogen in NH4+/NH3 attacks the electrophilic aldehyde group carbon of glycolaldehyde, initiating a C−N coupling reaction to form EOA60. The generated EOA is further oxidized to glycine by photogenerated holes58,59. During the photocatalytic process, the introduction of Pd−B diatomic sites, along with strong interaction between Pd and B atoms, significantly enhances the light absorption and accelerates electron transfer from B site to Pd site, thereby synergistically enhancing the redox capacity and facilitating the photocatalytic C−N coupling reaction towards glycine formation. More importantly, compared with conventional metal oxide photocatalysts, the Pd−B diatomic sites stabilize and drive the glycolaldehyde intermediate to complete the C−N coupling pathway while effectively suppressing its over-oxidation into byproducts, resulting in high selectivity for glycine production.

Photocatalytic conversion of gram-scale real-world PET plastic and nitrate to glycine
The photocatalytic conversion of gram-scale real-world PET plastic and nitrate to glycine was performed to validate the practical applicability. Glycine yields were achieved with 101.3 mmol g−1 cat (PET powder), 71.1 mmol g−1 cat (PET clothes), 89.4 mmol g−1 cat (PET bottle), 73.0 mmol g−1 cat (PET bag), 68.9 mmol g−1 cat (PET box) and 43.7 mmol g−1 cat (PET towel) using 10 g of real-world PET plastics as feedstock (Fig. 6a). Furthermore, this photocatalytic strategy demonstrates versatility, enabling its application to biomass-derived methanol and glycerol for photocatalytic C−N coupling reactions (Fig. 6b). When methanol and NO3− are used as reactants, the system selectively produced formamide. Conversely, when glycerol reacted with NO3−, glycine was successfully synthesized, possibly because glycerol can involve both the C−C bond cleavage step and the C−N coupling reaction. This finding further confirms the broad substrate applicability of this catalytic system, ranging from plastics to biomass. Furthermore, considering practical application scenarios, the impact of other ions commonly present in real environments on the reaction was evaluated. The introduction of ionic salts (Na+, Ca2+, Cl−, SO42-, HPO42-, etc.) and wastewater exerted varying degrees of inhibitory effects on glycine production. Despite this inhibition, the glycine yield remained above 33.2 mmol g−1 cat in real-world wastewater (Fig. 6c), preliminarily demonstrating the potential practical application of this system. Nevertheless, the composition of real wastewater is often highly complex and variable, and the application of the system necessitates further in-depth and systematic investigation. Future research efforts should focus on enhancing the resistance of the catalysts to interference and further optimizing reaction conditions to significantly improve the glycine yield.

A solar-driven photocatalytic glycine synthesis experiment was conducted by using a 30×30 cm2 plastic Fresnel lens as an economical and efficient solar concentrator (Fig. 6d). The results demonstrated that after 6 h of daily sunlight exposure, the glycine yield consistently remained between 10 and 20 mmol g−1 cat under different weather conditions, with selectivity maintained at 100% (Fig. 6e and Supplementary Fig. 24). Upon scaling up the reaction volume to 500 mL (Supplementary Fig. 25), the glycine yields further increased to 32.3 mmol g−1 cat. These findings confirm the feasibility of efficiently producing glycine using actual solar energy, laying a solid foundation for practical applications. Furthermore, the environmental impact of this catalytic system was also evaluated (Supplementary Fig. 26). Compared to conventional landfilling or incineration, processing 50,000 tons of waste plastic annually could prevent the emission of ~104 kt of CO2. Moreover, effective treatment of NO3− in industrial wastewater could prevent the release of ~147 kt of NO3− into groundwater or oceans annually. Consequently, this photocatalytic system not only holds significant potential for substantial commercial value but also demonstrates the possibility of achieving zero emissions for both CO2 and NO3−, contributing significantly to environmental sustainability.

Methods
Materials
Titanium dioxide (anatase TiO2, particle size <25 nm), zinc oxide (ZnO, particle size <50 nm), tungsten trioxide (WO3, particle size <50 nm), molybdenum disulfide (MoS2) and cadmium sulfide (CdS) were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., and characterized (Supplementary Fig. 27). Melamine, sodium hydroxide (NaOH), sodium cyanoborohydride (NaBH3CN), glyoxylic acid, ethanolamine and glycine were obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. Palladium(II) chloride (PdCl2), ethylene glycol (EG), glycolic acid (GA), methanol, glycerol, nitrobenzene, phenol, 5,5-dimethyl-1-pyrroline N-oxide (DMPO), ammonium chloride (NH4Cl), hexadecyl trimethyl ammonium bromide (CTAB) and activated carbon were purchased from Tianjin Xiensi Biochemical Technology Co., Ltd. Potassium nitrate (KNO3), sulfuric acid (H2SO4) and ammonium hydroxide (NH3·H2O) were acquired from Tianjin Damao Chemical Reagent Factory. Deuterium oxide (D2O, 99.90 at.% D) was purchased from Shanghai Meiruier Biochemical Technology Co., Ltd. All chemicals were used as received without further purification.

Synthesis of BCNx and CN catalysts
The cyano-group-enriched and boron-doped BCNx nanotubes were synthesized via thermal condensation of a supramolecular precursor consisting of melamine-cyanuric acid modified with sodium cyanoborohydride27,36. Typically, melamine (C3H6N6, 6.0 g, 47.57 mmol) was dissolved in 60 mL of distilled water and magnetically stirred for 30 min. The resultant suspension underwent hydrothermal treatment at a temperature of 180°C for a duration of 24 h in a 100 mL Teflon-lined stainless-steel autoclave. Subsequently, the mixture was allowed to cool to ambient temperature and then isolated through centrifugation, followed by thorough washing and vacuum drying overnight at 60°C. Thereafter, 100 mg of sodium cyanoborohydride (NaBH3CN) was dispersed in 6 mL of distilled water, along with 2.0 g of the dried supramolecular precursor, and the mixture was stirred continuously for a period of 2 h. The final mixture was then subjected to vacuum drying overnight at 60°C61. The obtained powder was uniformly spread in a 50 mL covered corundum porcelain boat and subjected to calcination under an Ar atmosphere with a heating rate of 5°C min−1 to reach 550°C, where the temperature was maintained for 4 h. The final material obtained was designated as BCNx. As a control experiment, a cyano-group-free and boron-free carbon nitride (CN) material was synthesized by directly calcining the supramolecular precursor without the addition of NaBH3CN.

Synthesis of Pd SA/BCNx and Pd SA/CN catalysts
Pd single-atom anchored BCNx and CN catalysts were synthesized via impregnation and thermal anchoring strategies. Typically, 100 mg of BCNx or CN was added to 10 mL of aqueous solution containing chloropalladic acid (H2PdCl6, 0.94 mM). The suspension was subjected to ultrasonication for 1 h, followed by stirring at room temperature for 4 h. Subsequently, the suspension was freeze-dried to obtain a solid powder. The resulting powder was uniformly spread in a 10 mL covered corundum porcelain boat and calcined under a N2 atmosphere with a heating rate of 5°C min–1 to reach 200°C, where the temperature was maintained for 1 h. The final products were designated as Pd SA/BCNx and Pd SA/CN, with a Pd loading of 1 wt%. For comparison, Pt SA/BCNx, Au SA/BCNx, Ag SA/BCNx, Cu SA/BCNx, Co SA/BCNx and Fe SA/BCNx with metal loadings of 1 wt% were also synthesized using the same procedure.

Synthesis of Pd NP/BCNx catalysts
Pd nanoparticles were loaded onto BCNx by the chemical reduction method, with a theoretical loading amount of 1.0 wt% Pd. The specific process is as follows: 100 mg of BCNx was added to 10 mL of aqueous solution of chloropalladic acid (H2PdCl6, 0.94 mM). Subsequently, under stirring conditions, a certain amount of NaBH4 solution (1 mol/L) was added at a Pd2+:NaBH4 molar ratio of 1:5. After reduction for 3 h, the precipitate was centrifuged and washed three times with deionized water and ethanol. The product was then dried overnight at 60°C to obtain the Pd NP/BCNx catalyst.

Hydrolysis of PET powder and collection of EG solution
The hydrolysis of commercial PET powder (~80 mesh) was carried out in a 250 mL single-neck flask. First, 4.2 g of PET powder and 0.2 g of cetyltrimethylammonium bromide (CTAB, as a phase-transfer catalyst, 0.55 mmol) were added to 200 mL of 0.1 M NaOH aqueous solution. The reaction system was stirred at 500 rpm and heated to 60°C to initiate the hydrolysis reaction. To maintain a constant pH in the reaction system, NaOH was continuously added dropwise until the PET was completely hydrolyzed, yielding equimolar amounts of terephthalic acid disodium salt (TPA-Na2) and EG monomers. After the reaction, activated carbon was added to adsorb CTAB and other impurities in the solution. The mixture was then acidified and filtered to isolate the TPA monomer. After the filtrate was neutralized, the resulting solution was the EG-rich aqueous solution.

Photocatalytic C−N coupling for glycine production
Photocatalytic C−N coupling experiments were conducted in a 100 mL sealed glass reactor at room temperature and atmospheric pressure using a 300 W Xe lamp (MicroSolar 300, Beijing Perfectlight Technology Co., Ltd., Supplementary Fig. 28). Typically, a certain amount of KNO3 was dissolved in the aforementioned EG aqueous solution to prepare a mixed solution containing EG (C donor, 0.1 M) and KNO3 (N donor, 0.5 M). The catalyst (20 mg) was dispersed in 50 mL of the EG/KNO3 mixed solution. Prior to illumination, the reactor was purged with Ar for 30 min to remove residual gases, and then irradiated from the top of the reaction cell. After 24 h of illumination (320 nm <λ<780 nm, 20 A Xe lamp current), the liquid phase products were analyzed via 1H NMR spectroscopy.

Photocatalytic C−N coupling of g-scale (10 g) real-world post-consumer PET plastics
The photocatalytic C−N coupling of g-scale real-world post-consumer PET plastics was also evaluated under the same conditions, except that 4.2 g of PET powder was replaced with 10 g of commercial PET materials, including PET clothes (PET content >85%), PET bottles (PET content >98%), PET bags (PET content >90%), PET boxes (PET content >85%) and PET towels (PET content >60%). Furthermore, the photocatalytic C−N coupling reactions using different alcohols (methanol and glycerol) as carbon sources were also assessed under the same reaction conditions.

Characterization and analytical methods
The morphology and structure of the catalysts were characterized via various techniques. Transmission electron microscopy (TEM) images were acquired using a JEM-F200 field emission transmission electron microscope. High-angle angular dark field-scanning transmission electron microscopy (HAADF-STEM) images were acquired using a JEM-ARM200F transmission electron microscope equipped with a probe-corrector operating at 200 kV. X-ray absorption fine structure (XAFS) analysis was performed at the Pd K-edge at the BL14W beamline of the Shanghai Synchrotron Radiation Facility (SSRF). The X-ray beam was monochromatized using a Si(311) crystal monochromator. Pd K-edge extended X-ray absorption fine structure (EXAFS) spectra were recorded in transmission mode. The EXAFS data was processed using Athena to handle background corrections as well as to calibrate both the pre-edge and post-edge features. Following this, Fourier transform fitting was performed in Artemis. For the analysis of Pd SA/BCNx, k3 weighting was utilized, with an R-range from 1 to 3 Å and a k-range set from 1.8 to 12 Å−162.

X-ray Diffraction (XRD) analysis was conducted using a SmartLab X-ray diffractometer (3 kW) equipped with Cu-Kα radiation. Fourier transform infrared spectroscopy (FT-IR) was conducted using a Nicolet Magna 670 infrared spectrometer. X-ray photoelectron spectroscopy (XPS) was conducted on a Thermo Scientific ESCALAB 250Xi. Solid-state 11B NMR spectra were recorded on an Agilent 600M spectrometer. UV-vis diffuse reflectance spectra were obtained with a Varian Cary 500 Scan UV-vis spectrophotometer, using BaSO4 as the reflectance standard. Photoluminescence (PL) and time-resolved photoluminescence (TR-PL) spectra were measured with an Edinburgh FLS1000 spectrophotometer. Transient photocurrent responses (TPR) and electrochemical impedance spectroscopy (EIS) were performed on an AutoLab-302N electrochemical workstation, utilizing a standard three-electrode system, with the catalyst-coated FTO (1×1 cm²), Ag/AgCl, and Pt electrodes serving as the working, reference, and counter electrodes, respectively. High-resolution gas chromatograph‒mass spectrometer (GC‒MS) analysis was performed using a Thermo Fisher-Q Exactive Plus system. In situ XPS spectra were acquired with a Thermo Fisher-ESCALAB 250Xi. In situ electron spin resonance (ESR) data was recorded using a Bruker-Emx Plus spectrometer. In situ attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) was performed with a Thermo Fisher-iS50 spectrometer. Femtosecond transient absorption spectra of the photocatalysts were acquired using a pump‒probe system (Helios, ultrafast system) equipped with a motorized optical delay line, allowing for time delays up to approximately 8 ns under ambient conditions. 1H NMR spectra were recorded on a 400 MHz Bruker spectrometer (AVANCE III HD 400) with D2O as the solvent and maleic acid (MA) as the internal standard. Quantitative analysis was performed via the following Eq. (1), and there are no products below the detection limit of the system.

n analyte = (S analyte / S standard) × (N standard / N monomer) × (M standard / M analyte) × 0.1 (1)

The conversion of EG was calculated via the following Eq. (2).

Conversion = (n0 - n analyte) / n0 × 100% (2)

The integral areas of the peaks for the analyte and MA are denoted as S analyte and S standard, respectively. The number of hydrogen atoms in the characteristic peak of the monomer used for quantitative analysis is represented by N monomer. The mass and molar mass of MA are indicated by m standard and M standard, respectively. V refers to the volume (50 mL) of the reaction mixture. n0 and n analyte represent the initial total molar amount and after-reaction molar amount of EG, respectively.

Determination of NO3−RR products in C−N coupling reaction
The NO3−RR products were identified and quantified using UV-vis spectroscopy. The determination details of NO2− and NH4+ using UV-vis are discussed as follows. (a) NO2−: The testing solution was prepared by combining 2 mL of the diluted reaction mixture with 1.0 mL of sulfanilamide solution (0.01 g/L in 10% H3PO4) and 1.0 mL of N-(1-naphthyl) ethylenediamine dihydrochloride solution (0.01 g/L). The resulting mixture was allowed to react at room temperature for 20 min before being analyzed by UV-vis spectroscopy. Mechanism of NO2− detection: the reaction of NO2− with sulfanilamide in a phosphate medium to form a diazonium salt, which then undergoes coupling with N-(1-naphthyl)-ethylenediamine dihydrochloride to generate a red dye. The absorbance is measured at a wavelength of 540 nm. (b) NH4+: The quantification of NH4+ was performed using the indophenol blue method. After completing the reaction, 2.0 mL of the diluted electrolyte was mixed with a solution containing NaOH (1.0 M), salicylic acid (5.0 wt.%), sodium citrate (2.0 mL, 5.0 wt.%), and NaClO (1.0 mL, 0.05 M), followed by the addition of 0.20 mL of sodium nitroprusside solution (1.0 wt.%). The mixture was then allowed to stabilize for 2 h before UV-vis analysis. Mechanism of NH4+ detection: under alkaline conditions, NH4+ first reacts with NaClO to form NO2−, which then reacts with salicylic acid to produce C7H5NO3−. Finally, this compound undergoes coupling with sodium citrate and sodium nitroprusside to form an azo compound, which exhibits a specific absorbance peak at 651 nm.

DFT calculations
Spin-polarized periodic DFT calculations were performed via the Vienna ab initio simulation package (VASP, version 5.4.4)63,64. The electron-ion interaction was described via the projector augmented wave (PAW)65,66. The electron exchange and correlation energies were calculated based on the Perdew–Burke–Ernzerhof (PBE) functional67, and the effects of van der Waals interactions were investigated based on Grimme’s DFT-D3 method68. A plane-wave cut-off energy of 400 eV was applied for all DFT calculations.

For the model construction, a 2×2 anatase-TiO2 (001) surface slab was established with twelve atomic layers (four O‒Ti‒O layers). The Pd single atom was embedded into the boron-doped C3N4 monolayer to simulate the Pd SA/BCN sample (Supplementary Data 1). During structure optimization, the six atomic layers on the bottom of the TiO2 slab were fixed, while other atoms, including the adsorbates, were allowed to relax. For the Pd SA/BCN, relaxation was performed for all the atoms in the system. The structural relaxation was continued until the force on each atom was less than 0.02 eV/Å. The adsorption energy was defined as follows Eq. (3).

E ads = E adsorbate/slab - E adsorbate - E slab (3)

where E adsorbate/slab, E adsorbate, and E slab are the total energies of the optimized slab with adsorbates, the adsorbates, and the clean slab, respectively.

Peer review rounds
[{"round": 1, "reviewer_comments": {"reviewer_1": "This study reports a Pd–B diatomic catalyst that enables the selective photocatalytic synthesis of glycine via C–N coupling between waste PET-derived ethylene glycol (EG) and nitrate. The catalyst achieves a glycine production rate of 2.9 mmol gcat⁻¹ h⁻¹ with 92% selectivity, outperforming previously reported systems. Mechanistic studies reveal that EG is photo-oxidized at the hole-rich B site to form glycolaldehyde as the key intermediate, while nitrate is photo-reduced to NH₄⁺ at the electron-rich Pd site. Subsequent C–N coupling between glycolaldehyde and NH₄⁺ produces ethanolamine, which is further photo-oxidized to glycine. Moreover, the catalytic system is extendable to gram-scale conversion of real PET plastics and biomass-derived polyols under natural sunlight. In general, the experimental results are interesting and suitable for the journal. However, minor revisions are still required, and we outline several suggestions that could further improve the manuscript.\n1. The authors conducted fs-TAS measurements on Pd SA/BCNx and BCNx. Could the average relaxation lifetimes of the two samples be analyzed to further compare their charge separation efficiency? (Hangxun Xu, et al., Adv. Mater. 2021, 2107480).\n2. The authors confirmed that the catalyst is capable of generating ·OH radicals and that the introduction of ethylene glycol suppresses their formation. Does the catalyst also possess the ability to generate O₂? If so, would O₂ generation also be suppressed upon the addition of ethylene glycol?\n3. In Figure 5k, the mechanistic scheme describes that photogenerated holes oxidize ethylene glycol and ethanolamine, while photogenerated electrons reduce water to H₂ instead of participating in nitrate reduction. However, no experimental evidence is provided for this process. The authors are encouraged to carefully examine and clarify this part.\n4. g-C₃N₄ is known to be an efficient photocatalyst for producing H₂O₂ and ·O₂ (Takayuki Hirai, et al., ACS Catal. 2015, 5, 3058–3066), while Pd catalysts are effective cocatalysts for H₂O₂ generation (Chiheng Chu, Nat. Commun. 2022, 13, 7783). In this system, is there any evidence for the generation of H₂O₂ or ·O₂? If so, could they also contribute to the oxidation of ethylene glycol?\n5. There are some writing and grammatical issues, for example, on page 15: “…that then that glycolaldehyde would then undergo a preliminary…” and the use of NO₃--. The authors are advised to carefully check and revise the manuscript throughout.", "reviewer_2": "This manuscript reports glycine photosynthesis via C−N coupling of waste PET-derived ethylene glycol and nitrate on Pd−B diatomic catalyst. Experiments were conducted well and the conclusion can be well supported by data. The catalytic performance on glycine photosynthesis is superior to the previously reported catalysts. The mechanism analysis is well implemented. The Pd-B dual site synergy effect during the C-N coupling is inspiring in this field. Especially, PET-derived EG conversion to glycine is a highly promising strategy to address waste plastics crisis and obtain high value chemicals. Based on the above points, I suggest the acceptance of this manuscript to be published on Nature Communications after addressing the following problems.\n1. TAS signals of (g) Pd SA/BCNx and (h) BCNx on the ps-ns timescale are very weak. To make sound conclusion, it is suggested to recollect TA spectra.\n2. The conversion ratio of EG and nitrate is suggested to be provided.\n3. The B and Pd contents in the catalyst are suggested to be given.\n4. Calculations on the reaction pathway to elucidate the high intrinsic activity in C-N coupling and selective glycine production are suggested to be conducted.", "reviewer_3": "The manuscript deals with the Glycine photosynthesis via C−N coupling of waste PET-derived ethylene glycol and nitrate on Pd−B diatomic catalyst.\nThe information and topic reported are very interesting and the catalysts are well characterized.\nOnly some minor comments should be addressed:\n1) What about the integration of this process with the PET recycling processes? What are the advantages and the drawbacks and how about the cost compared to the other processes?\n2) It is possible that during the reaction it was verified the PET photoreforming with H2 production? Please explain\n3)The Pd is a critical materials. What about the possibility to use other not critical or transition metals?"}, "author_response": "General Comment R1: This study reports a Pd–B diatomic catalyst that enables the selective photocatalytic synthesis of glycine via C–N coupling between waste PET-derived ethylene glycol (EG) and nitrate. The catalyst achieves a glycine production rate of 2.9 mmol g -1 h-1 cat with 92% selectivity, outperforming previously reported systems. Mechanistic studies reveal that EG is photo-oxidized at the hole-rich B site to form glycolaldehyde as the key intermediate, while nitrate is photo-reduced to NH₄⁺ at the electron-rich Pd site. Subsequent C–N coupling between glycolaldehyde and NH₄⁺ produces ethanolamine, which is further photo-oxidized to glycine. Moreover, the catalytic system is extendable to gram-scale conversion of real PET plastics and biomass-derived polyols under natural sunlight. In general, the experimental results are interesting and suitable for the journal. However, minor revisions are still required, and we outline several suggestions that could further improve the manuscript.\nResponse: We thank the reviewer for the positive comments and valuable suggestions, which will greatly improve our manuscript. We have made additional modifications and discussions according to your suggestions in the revised manuscript.\nSpecific Comments R1-1: The authors conducted fs-TAS measurements on Pd SA/BCNx and BCNx. Could the average relaxation lifetimes of the two samples be analyzed to further compare their charge separation efficiency? (Hangxun Xu, et al., Adv. Mater. 2021, 2107480).\nResponse: We acknowledge the helpful suggestion of the reviewer. We have supplemented Normalized TA kinetic curves (Figure 4h) and fitted the average ground-state bleaching (GSB) recovery times (Table S5) of two samples in manuscript to further confirm the charge transfer efficiency. The kinetic analysis of the absorption decay curve reveals the average ground-state bleaching (GSB) recovery times of BCNx (314.88 ps) and Pd SA/BCNx (264.12 ps), respectively (Figure 4h, Table S5), suggesting efficient the photoelectron transfer from the BCNx to the Pd atom (Figure 4i), consistent with the reported results (J. Am. Chem. Soc. 2025, 147, 38484; Adv. Mater. 2025, 37, 2412965; J. Am. Chem. Soc. 2024, 146, 21008).\nFigure 4. (h) Normalized TA kinetic curves of BCNx and Pd SA/BCNx.\nSample τ (ps) B (%) τ (ps) B (%) τ (ps) B (%) τ (ps)\n1 1 2 2 3 3 ave\nBCNx 0.27 45.09 9.69 30.35 326.55 24.56 314.88\nPd SA/BCNx 0.11 51.81 7.69 23.12 271.05 25.07 264.12\nWe have added these discussions to our manuscript:\nFemtosecond transient absorption spectroscopy (fs-TAS) was employed to investigate the photoelectron transfer kinetics of Pd SA/BCNx and BCNx (Figure 4d-g). Upon excitation with a 400 nm pump pulse, both Pd SA/BCNx and BCNx are excited from the ground state to the excited state, with the probe wavelength ranging from 410 nm to 750 nm. The TAS of BCNx exhibits a distinct negative peak at ~440 nm (Figure 4e,g), corresponding to ground-state bleaching, indicating the relaxation process of the excited state. In contrast, the signal intensity at 440 nm for Pd SA/BCNx is significantly weaker than that of BCNx, and an additional negative peak at ~560 nm is observed at early delay times (Figure 4d,f). The kinetic analysis of the absorption decay curve reveals the average ground-state bleaching (GSB) recovery times of BCNx (314.88 ps) and Pd SA/BCNx (264.12 ps), respectively (Figure 4h, Table S5), suggesting efficient the photoelectron transfer from the BCNx to the Pd atom (Figure 4i).53\n53. Chen, H.-Y., Zhao, Z.-H., Huang, N.-Y., Huang, J.-R. & Liao, P.-Q. Rational Design and Synthesis of a Metal–Organic Framework Featuring Cu(I)–Carbon Bonds for Enhanced Artificial Photosynthesis. J. Am. Chem. Soc. 147, 38484-38491 (2025).\nSpecific Comments R1-2: The authors confirmed that the catalyst is capable of generating ·OH radicals and that the introduction of ethylene glycol suppresses their formation. Does the catalyst also possess the ability to generate O ? If so, would O₂ generation also be suppressed upon the addition of ethylene glycol?\nResponse: Thank you for your constructive suggestion. In the revised manuscript, we have included additional experiments to detect the gas-phase products in different systems (Figure S18). We found that in pure water systems, photocatalytic overall water splitting reactions occur, producing H and O . As anticipated, in EG and EG/NO - solutions, O production is 2 2 3 2 suppressed. This is because the oxidation of EG molecules takes precedence over water oxidation, thereby consuming the photogenerated holes and generating alkyl radicals, which are crucial for the C-N coupling process.\nIn addition, no •O - or H O is produced in the pure H O, EG and EG/NO - systems, 2 2 2 2 3 indicating that the oxidation reaction is only driven by the photogenerated holes (Figure S17). Subsequently, comparing the gas-phase products in the three systems (Figure S18), it is found in the pure H O system, Pd SA/BCNx is more inclined to undergo photocatalytic overall H O 2 2 splitting, producing trace amounts of H and O . In the EG aqueous solution system, the EG 2 2 molecules more readily consume the holes, causing Pd SA/BCNx to generate more photogenerated electrons for reducing H O to H . However, in the EG/ NO - system, no 2 2 3 gaseous products are detected, suggesting that the photogenerated electrons are exclusively utilized for the reduction of NO -. The above results indicate that EG molecules can be 3 effectively activated to form •CHOHCH OH through dehydrogenation, thereby generating an 2 H-C=O precursor for the subsequent C−N coupling.40 Therefore, photogenerated holes predominantly drive the oxidation of EG to generate alkyl radicals, whereas photogenerated electrons mainly participate in the reduction of NO - to form nitrogen intermediates (NO -, 3 2 NH , or NH +). The generation of alkyl radicals and nitrogen intermediates creates favourable 3 4 conditions for the subsequent C−N coupling reaction.\nSpecific Comments R1-3: In Figure 5k, the mechanistic scheme describes that photogenerated holes oxidize ethylene glycol and ethanolamine, while photogenerated electrons reduce water to H₂ instead of participating in nitrate reduction. However, no experimental evidence is provided for this process. The authors are encouraged to carefully examine and clarify this part.\nResponse: We are grateful to the reviewer for the valuable comment. Regarding the oxidation reaction in C-N coupling, we have supplemented our findings with UV-vis spectroscopy and ESR spectra to demonstrate that no H O or ·O - species are generated in the system (Figure 2 2 2 S17). Thus, the oxidation reaction in the C-N coupling process is primarily driven by photogenerated holes. For the oxidation of EG, in situ ESR measurements confirm that photogenerated holes oxidize ethylene glycol to produce carbonyl radicals (•CHOHCH OH) 2 (Figure 5d). For the oxidation of ethanolamine (EOA), a comparison of glycine yields is 4 performed over CN, BCNx, Pd SA/CN, and Pd SA/BCNx, showing that the glycine yield is significantly higher for catalysts containing B, with BCNx even outperforming Pd SA/CN. This observation further supports that the oxidation of EOA predominantly occurs at the photogenerated hole-rich B sites (Figure S22). For the reduction reaction in C-N coupling, we have also provided analysis of the gaseous products in the system (Figure S18). In the EG solution, Pd SA/BCNx utilizes photogenerated electrons to reduce H O, generating a 2 substantial amount of H . However, no gaseous products are observed in the EG/NO - 2 3 solution system. Nevertheless, reduction products (NO -, NH +) of NO - are detectable via 2 4 3 UV-vis spectroscopy (Figure 5g,h). These results confirm that in the C-N coupling system, the photogenerated electrons are exclusively involved in the reduction of NO -.\nFigure S17. (a) ESR spectra of •O - generated after 10 min of illumination over Pd SA/BCNx 2 in pure H O, EG and EG/NO -. (b) UV‒vis spectra of H O after photocatalytic reaction at 24 2 3 2 2 h over Pd SA/BCNx in pure H O, EG and EG/NO -.\n2 3\nFigure 5. UV‒vis spectra of (g) NO - and (h) NH + in the photocatalytic reaction solution at 2 4 24 h and 48 h over Pd SA/BCNx.\nWe have added these discussions to our manuscript:\nWhen both EG and NO - are present simultaneously, the •CHOHCH OH signal peak further 3 2 intensifies, suggesting that NO - is preferentially reduced over water to consume more 3 photogenerated electrons, further demonstrating that the generation of •CHOHCH OH is 2 directly related to the increase in glycine yield. In addition, no •O - or H O is produced in the 2 2 2 pure H O, EG and EG/NO - systems, indicating that the oxidation reaction is only driven by 2 3 the photogenerated holes (Figure S17). Subsequently, comparing the gas-phase products in the three systems (Figure S18), it is found in the pure H O system, Pd SA/BCNx is more 2 inclined to undergo photocatalytic overall H O splitting, resulting in trace amounts of H and 2 2 O . In the EG aqueous solution system, the EG molecules more readily consume the holes, 2 causing Pd SA/BCNx to generate more photogenerated electrons for reducing H O to H . 2 2 However, in the EG/ NO - system, no gaseous products are detected, suggesting that the 3 photogenerated electrons are exclusively utilized for the reduction of NO -. The above results 3 indicate that EG molecules can be effectively activated to form •CHOHCH OH through 2 dehydrogenation, thereby generating an H-C=O precursor for the subsequent C−N coupling.40 Therefore, photogenerated holes predominantly drive the oxidation of EG to generate alkyl radicals, whereas photogenerated electrons mainly participate in the reduction of NO - to form 3 nitrogen intermediates (NO -, NH , or NH +). The generation of alkyl radicals and nitrogen 2 3 4 intermediates creates favourable conditions for the subsequent C−N coupling reaction.\nSpecific Comments R1-4: g-C N is known to be an efficient photocatalyst for producing 3 4 H O and ·O (Takayuki Hirai, et al., ACS Catal. 2015, 5, 3058–3066), while Pd catalysts are 2 2 2 effective cocatalysts for H O generation (Chiheng Chu, Nat. Commun. 2022, 13, 7783). In 2 2 this system, is there any evidence for the generation of H O or ·O ? If so, could they also 2 2 2 contribute to the oxidation of ethylene glycol?\nResponse: We acknowledge the helpful suggestion of the reviewer. In the manuscript, we have added ESR (Figure S17a) and UV-vis spectroscopic tests (Figure S17b), and no detectable signals for ·O - and H O species were observed. This is because the C-N coupling 2 2 2 reaction in this study was carried out under an argon atmosphere, which makes it unlikely for the oxygen reduction reaction (ORR) to produce H O or ·O -. Therefore, the oxidation of 2 2 2 ethylene glycol was solely driven by photogenerated holes.\nFigure S17. (a) ESR spectra of •O - generated after 10 min of illumination over Pd SA/BCNx 2 in pure H O, EG and EG/NO -. (b) UV‒vis spectra of H O after photocatalytic reaction at 24 2 3 2 2\n7\nh over Pd SA/BCNx in pure H O, EG and EG/NO -.\n2 3\nWe have added these discussions to our manuscript:\nWhen both EG and NO - are present simultaneously, the •CHOHCH OH signal peak further 3 2 intensifies, suggesting that NO - is preferentially reduced over water to consume more 3 photogenerated electrons, further demonstrating that the generation of •CHOHCH OH is 2 directly related to the increase in glycine yield. In addition, no •O - or H O is produced in the 2 2 2 pure H O, EG and EG/NO - systems, indicating that the oxidation reaction is only driven by 2 3 the photogenerated holes (Figure S17).\nSpecific Comments R1-5: There are some writing and grammatical issues, for example, on page 15: “…that then that glycolaldehyde would then undergo a preliminary…” and the use of NO --. The authors are advised to carefully check and revise the manuscript throughout.\nGeneral Comment R2: This manuscript reports glycine photosynthesis via C−N coupling of waste PET-derived ethylene glycol and nitrate on Pd−B diatomic catalyst. Experiments were conducted well and the conclusion can be well supported by data. The catalytic performance on glycine photosynthesis is superior to the previously reported catalysts. The mechanism analysis is well implemented. The Pd-B dual site synergy effect during the C-N coupling is inspiring in this field. Especially, PET-derived EG conversion to glycine is a highly promising strategy to address waste plastics crisis and obtain high value chemicals. Based on the above points, I suggest the acceptance of this manuscript to be published on Nature Communications after addressing the following problems.\nResponse: We are glad that the reviewers saw value in our approach and recognized the significance of our project. We also thank the viewer for the valuable suggestions, which will greatly improve our manuscript. We have made additional modifications or discussions according to the reviewer’s suggestions in the revised manuscript.\nSpecific Comments R2-1: TAS signals of (g) Pd SA/BCNx and (h) BCNx on the ps-ns timescale are very weak. To make sound conclusion, it is suggested to recollect TA spectra.\nResponse: We acknowledge the helpful suggestion of the reviewer. We have conducted multiple tests to obtain the TAS signals of two samples, and selected the highest quality dataset (Figure 4f,g). In fact, for carbon nitride materials, the TAS signal is generally relatively weak (J. Am. Chem. Soc. 2020, 142, 2795; ACS Catal. 2024, 14, 7506; Appl. Catal. B 2024, 341, 123334), and many instruments struggle to provide satisfactory results. Additionally, we also included normalized TA dynamics curves (Figure 4h) and fitted the average ground-state bleaching (GSB) recovery times (Table S5) of the two samples in the manuscript to further confirm that Pd atoms optimize the charge transfer efficiency, consistent with the reported results (J. Am. Chem. Soc. 2025, 147, 38484; Adv. Mater. 2025, 37, 2412965; J. Am. Chem. Soc. 2024, 146, 21008).\nFigure 4. TAS signals of (d) Pd SA/BCNx and (e) BCNx on the ps-ns timescale. 2D mapping 9 fs-TAS of (f) Pd SA/BCNx and (g) BCNx. (h) Normalized TA kinetic curves of BCNx and Pd SA/BCNx. (i) Photoelectron transfer pathway between the atomic Pd site and the B site in Pd SA/BCNx.\nTable S5. Calculated decay lifetimes from corresponding fs-TAS kinetic traces.\nSample τ (ps) B (%) τ (ps) B (%) τ (ps) B (%) τ (ps)\n1 1 2 2 3 3 ave\nBCNx 0.27 45.09 9.69 30.35 326.55 24.56 314.88\nPd SA/BCNx 0.11 51.81 7.69 23.12 271.05 25.07 264.12\nWe have added these discussions to our manuscript:\nFemtosecond transient absorption spectroscopy (fs-TAS) was employed to investigate the photoelectron transfer kinetics of Pd SA/BCNx and BCNx (Figure 4d-g). Upon excitation with a 400 nm pump pulse, both Pd SA/BCNx and BCNx are excited from the ground state to the excited state, with the probe wavelength ranging from 410 nm to 750 nm. The TAS of BCNx exhibits a distinct negative peak at ~440 nm (Figure 4e,g), corresponding to ground-state bleaching, indicating the relaxation process of the excited state. In contrast, the signal intensity at 440 nm for Pd SA/BCNx is significantly weaker than that of BCNx, and an additional negative peak at ~560 nm is observed at early delay times (Figure 4d,f). The kinetic analysis of the absorption decay curve reveals the average ground-state bleaching (GSB) recovery times of BCNx (314.88 ps) and Pd SA/BCNx (264.12 ps), respectively (Figure 4h, Table S5), suggesting efficient the photoelectron transfer from the BCNx to the Pd atom (Figure 4i).53\n53. Chen, H.-Y., Zhao, Z.-H., Huang, N.-Y., Huang, J.-R. & Liao, P.-Q. Rational Design and Synthesis of a Metal–Organic Framework Featuring Cu(I)–Carbon Bonds for Enhanced Artificial Photosynthesis. J. Am. Chem. Soc. 147, 38484-38491 (2025).\nSpecific Comments R2-2: The conversion ratio of EG and nitrate is suggested to be provided.\nResponse: We thank the reviewer very much for the valuable comments. The conversion rates of EG and nitrate have been incorporated into the manuscript (Figure S9). To achieve a high glycine yield, we added sufficient or excess EG and nitrate, therefore, within 24 h, the conversion rates of EG and nitrate are 23.4% and 5.6%, respectively. Furthermore, by calculating the consumption of EG and the total content of the products, it was found that the reaction adheres to carbon balance, with EG being fully converted into approximately 92% glycine and approximately 8% glycolic acid (GA).\nThe conversion efficiency of EG and NO - on the Pd SA/BCNx catalyst are 23.4% and 5.6%, 3 respectively, within 24 h (Figure S9), and the total molar amount of detected products (glycine and GA) was approximately equal to the amount of consumed EG.\nSpecific Comments R2-3: The B and Pd contents in the catalyst are suggested to be given.\nResponse: We acknowledge the helpful suggestion of the reviewer. We have conducted quantitative X-ray photoelectron spectroscopy (XPS) analysis, EDS elemental analysis and inductively coupled plasma optical emission spectrometry (ICP-OES) on the B and Pd elements in the Pd SA/BCNx in the manuscript. The relevant content has been added to the manuscript.\nTable S1. Elemental content analysis (wt%) for Pd SA/BCNx based on the XPS, EDS and ICP tests.\nMethod B Pd\nXPS 1.37 1.04\nEDS 1.24 0.98\nICP / 1.07\nWe have added these discussions to our manuscript:\nX-ray photoelectron spectroscopy (XPS) further confirms the chemical and oxidation states of C, N, B and Pd elements in Pd SA/BCNx and the doping content of B and Pd is approximately 1.37 wt% and 1.04 wt%, respectively (Figure S2, Table S1). The doping content of B and Pd is also further determined through EDS elemental analysis (B:1.24 wt%; Pd:0.98 wt%) and inductively coupled plasma optical emission spectrometry (ICP-OES) (Pd:1.07 wt%).\nResponse: We acknowledge the helpful suggestion of the reviewer. We conducted the key elementary step of glycine production on Pd SA/BCNx and TiO , namely the C-N coupling 2 between *CH OHCHO and *NH (Nat. Commun., 2025, 16, 8040) (Figure S23d) in the 2 2 manuscript. *CH OHCHO and *NH species are adsorbed on a single Pd site on Pd 2 2 SA/BCNx and associated to the *CH OHCHONH with an activation barrier of 0.18 eV. In 2 2 contrast, the *CH OHCHO and *NH species are adsorbed on two neighboring Ti sites on 2 2 TiO , requiring 0.47 eV to activate the C−N bond formation. Meanwhile, the active lattice 2 oxygen on TiO is known to oxidize the adsorbed species, such as the −C=O or −CHO group 2 (Figure S23e) (Nat. Commun., 2024, 15, 6827). Therefore, compared with TiO , the Pd 2 SA/BCNx catalyst preferred the C−N coupling step to further produce the glycine. These results provide a rational theoretical explanation for the high selectivity of Pd SA/BCNx for glycine production.\nFigure S23. (a) Proposed reaction pathway. Reaction energy of glycolaldehyde intermediate to (b) GA and (c) EOA on TiO and Pd SA/BCNx model catalysts. (d) The reaction potential 2 energy diagram of C-N coupling between *CH OHCHO and *NH on Pd SA/BCNx and TiO . 2 2 2 (e) Configuration of *CH OHCHO on the surface of TiO .\n2 2\nWe have added these discussions to our manuscript:\nIn addition to the spectroscopic evidence mentioned above, density functional theory (DFT) calculations were employed to investigate the reaction energies for two pathways over TiO 2 and Pd SA/BCNx. The conversion of glycolaldehyde to GA was found to possess a larger negative reaction energy on TiO than Pd SA/BCNx, indicating that TiO is more favourable 2 2 for the oxidation of EG to GA (Pathway 1), which is further oxidized to produce FA by-products (Figure S23b). In contrast, the Pd SA/BCNx surface is found to favor the amination of glycolaldehyde to form EOAs (Figure S23c), which is further oxidized to glycine (Pathway 2). Subsequently, we also conducted the key fundamental step of glycine production on Pd SA/BCNx and TiO , specifically the C-N coupling between *CH OHCHO and *NH (Figure 2 2 2 S23d).52 *CH OHCHO and *NH species are adsorbed on a single Pd site on Pd SA/BCNx 2 2 and associated to the *CH OHCHONH with an activation barrier of 0.18 eV. In contrast, the 2 2 *CH OHCHO and *NH species are adsorbed on two neighboring Ti sites on TiO , requiring 2 2 2 0.47 eV to activate the C−N bond formation. Meanwhile, the active lattice oxygen on TiO is 2 known to oxidize the adsorbed species, such as the −C=O or −CHO group (Figure S23e).53 Therefore, compared with TiO , the Pd SA/BCNx catalyst preferred the C−N coupling step to 2 further produce the glycine. These results provide a rational theoretical explanation for the high selectivity of Pd SA/BCNx for glycine production.\n52. Wang, X. Z. et al. Revisiting Pt foil catalysts for formamide electrosynthesis achieved at industrial-level current densities. Nat. Commun. 16, 8084(2025).\n53. Zhang, T. F. et al. Simultaneously activating molecular oxygen and surface lattice oxygen on Pt/TiO for low-temperature CO oxidation. Nat, Commun, 15, 6827 (2024).\n2\nGeneral Comment R3: The manuscript deals with the Glycine photosynthesis via C−N coupling of waste PET-derived ethylene glycol and nitrate on Pd−B diatomic catalyst. The information and topic reported are very interesting and the catalysts are well characterized. Only some minor comments should be addressed:\nResponse: We thank the reviewer for the positive comments and valuable suggestions, which will greatly improve our manuscript. We have made additional modifications and discussions according to the reviewer’s suggestions in the revised manuscript.\nSpecific Comments R3-1: What about the integration of this process with the PET recycling processes? What are the advantages and the drawbacks and how about the cost compared to the other processes?\nResponse: We thank the reviewer very much for the valuable comments. The current environmentally friendly method for the chemical recycling of PET involves hydrolyzing PET to depolymerize it into TPA and EG monomers. This resultant EG solution can be directly utilized in C-N coupling reactions to synthesize high-value glycine (with a market price of $4,600 per ton). This process can be effectively integrated with the chemical hydrolysis of PET, thereby enhancing the valorization of EG. This is primarily due to the fact that \"pure EG\" is employed during PET production, and if the EG solution obtained through hydrolysis were to be reused for PET production, it would require purification. The manuscript notes: \"the distillation separation and purification of EG is relatively difficult and costly due to its high boiling point (197.3°C) and water solubility.\" Consequently, the purification of EG is a high-energy and high-cost process. Moreover, given the relatively low market price of EG ($800 per ton) and its widespread availability, it is even less cost-effective to purify the hydrolyzed EG solution for reuse in PET production. Most commercially available PET recycling companies predominantly recycle TPA, with little emphasis on reusing EG for PET synthesis, largely due to cost considerations and the challenges in achieving the purity levels required for re-polymerization (especially for bottle-grade standards). In summary, it appears more economical to convert the EG solution derived from PET hydrolysis into high-value chemicals, such as glycolic acid (GA) and amino acids.\nThis study adopts a glycine production process to treat EG solutions derived from PET and NO - from wastewater, representing a more economical and efficient option. However, 3 the scalability of photocatalytic glycine synthesis still requires significant improvement. Furthermore, compared to existing wastewater treatment methods, our approach can only be said to utilize nitrate from wastewater rather than addressing wastewater treatment per se, as the composition of wastewater is highly complex. Thus, our research may be considered more of a concept and vision at this stage. In our manuscript, we emphasize, \"Nevertheless, the composition of real wastewater is often highly complex and variable, and the application of the system necessitates further in-depth and systematic investigation. Future research efforts should focus on enhancing the resistance of the catalysts to interference and further 14 optimizing reaction conditions to significantly improve the glycine yield.\"\nRegarding the cost of this process, we conducted a preliminary TEA analysis of the traditional PET recycling process and this integration process, and the results show significant market value for this integration process with a profit of $73.0 million per year for treating 50,000 tons of plastic waste (Table 1). This economic benefit substantially surpasses that of traditional chemical depolymerization processes ($3.4 million) (Table 2), primarily due to the synergistic production of high-value products, such as glycine, TPA and GA, suggesting that the upgrading system holds substantial commercial value and practical application prospects over chemical recycling alone.\nTable 1. Techno-economic analysis based on a capability of 50000 tons of PET waste per year for upgrading to glycine.\nItems Total price\n(million USD)\nDepreciation -10.0\nUtility cost -8.4\nMaintenance cost -10.0\nLabor cost -10.0\nChemicals Weight per ton (ton) Unit price (USD ton-1)\nPET\nWaste PET 1.000 333.9 -16.7\nSodium hydroxide 0.416 340.0 -7.0\n(NaOH)\nSulfuric acid 0.510 92.7 -2.4\n(H SO )\n2 4\nPotassium nitrate 0.483 481.6 -11.6\n(KNO )\n(TPA)\nGlycine 0.359 4640.8 83.4\nGlycolic acid 0.031 20000 31.0\n(GA)\nNet profit 73.0\nItems Total price\n(million USD)\nDepreciation -5.0\nUtility cost -4.2\nMaintenance cost -5.0\nLabor cost -5.0\nChemicals Weight per ton (ton) Unit price (USD ton-1)\nPET\nWaste PET 1.000 333.9 -16.7\nSodium hydroxide 0.416 340.0 -7.0\n(NaOH)\nSulfuric acid 0.510 92.7 -2.4\n(H SO )\n2 4\nTerephthalic acid 0.864 803.7 34.7\n(TPA)\nEthylene glycol 0.323 865.5 14.0\n(EG)\nNet profit 3.4\nWe have added these discussions to our manuscript:\nPoly(ethylene terephthalate) (PET), as the most produced and widely consumed polyester plastic globally, boasts an annual output of approximately 100 million tons, primarily synthesized through the polymerization of terephthalic acid (TPA) and ethylene glycol (EG).24 However, over 80% of PET waste ends up in landfills or is discharged into the environment, posing a severe threat to ecosystems and resulting in significant carbon resource waste.25,26 Chemical hydrolysis of PET into TPA and EG monomers is generally regarded as a highly promising recycling approach (Figure 1c).27,28 Nevertheless, in practical implementation, the separation and purification of EG via distillation are operationally complex and costly due to its high boiling point (197.3 °C) and water solubility.29 Moreover, given the relatively low market price of EG ($800 per ton) and its widespread availability, it is even less cost-effective to purify the hydrolyzed EG solution for reuse in PET production. Instead, photocatalytic oxidation of PET-derived EG aqueous solutions into higher-value-added products, such as glycolic acid (GA, $20000 per ton), acetic acid (AA, $3000 per ton) or formic acid (FA, $900 per ton), has emerged as a highly attractive alternative pathway (Figure 1c).30-33 However, the significant challenge of this photocatalytic process is its low selectivity and a limited product scope, primarily acids.34 Developing a strategy to expand the product range will help to reintroduce waste carbon from plastics into the utilization cycle. Sufficient evidence has 16 demonstrated that the electrophilic glycolaldehyde serves as a key intermediate in the photooxidation of EG to form organic acids.32,35,36 Therefore, developing a catalyst capable of effectively stabilizing and capturing electrophilic glycolaldehyde intermediates during EG photo-oxidation to achieve selective C−N coupling with nitrate for photosynthesis of high-value glycine ($4600 per ton) would be a highly promising and scientifically valuable research strategy.\nSpecific Comments R3-2: It is possible that during the reaction it was verified the PET photoreforming with H production? Please explain.\nFigure S18. Photocatalytic gas-phase product production of Pd SA/BCNx in pure H O, EG, 2 and EG/NO - systems within 24 h.\nSubsequently, comparing the gas-phase products in the three systems (Figure S18), it is found in the pure H O system, Pd SA/BCNx is more inclined to undergo photocatalytic overall H O 2 2 splitting, resulting in trace amounts of H and O . In the EG aqueous solution system, the EG 2 2 molecules more readily consume the holes, causing Pd SA/BCNx to generate more photogenerated electrons for reducing H O to H . However, in the EG/ NO - system, no 2 2 3 gaseous products are detected, suggesting that the photogenerated electrons are exclusively utilized for the reduction of NO -. The above results indicate that EG molecules can be 3 effectively activated to form •CHOHCH OH through dehydrogenation, thereby generating an 2 H-C=O precursor for the subsequent C−N coupling.40 Therefore, photogenerated holes 17 predominantly drive the oxidation of EG to generate alkyl radicals, whereas photogenerated electrons mainly participate in the reduction of NO - to form nitrogen intermediates (NO -, 3 2 NH , or NH +). The generation of alkyl radicals and nitrogen intermediates creates favourable 3 4 conditions for the subsequent C−N coupling reaction.\nSpecific Comments R3-3: The Pd is a critical materials. What about the possibility to use other not critical or transition metals?\nResponse: We acknowledge the helpful suggestion of the reviewer. We have supplemented the data with additional metal atoms (Pt, Pd, Au, Ag, Cu, Co, and Fe) in the context of photosynthetic glycine production (Figure 3c). The updated data further highlight the significance of Pd in this reaction. Cu atoms do not enhance the production activity of glycine. Pt and Fe atoms lead to the formation of acetic acid by-products, thus reducing the product selectivity towards glycine. On the other hand, Pd, Au, Ag, and Co atoms improve the activity of glycine production without compromising its selectivity, with Pd demonstrating the highest activity among them.\nFigure 3. (c) Glycine production rates over various metal single-atom anchored BCNx catalysts, including Pt, Pd, Au, Ag, Cu, Co and Fe.\nFigure S6. 1H NMR spectra of photocatalytic of 0.1 M EG/0.5 M KNO mix solution after 24 3 h by (b) different metal (Pt, Pd, Au, Ag, Cu, Co and Fe) single atom anchored BCNx catalysts.\nSpecifically, different single metal atoms (Pt, Pd, Au, Ag, Cu, Co and Fe) were further anchored on the BCNx support to construct dual active site catalysts to increase glycine production activity (Figure 3c, Figure S6b). The catalytic performance reveals that the Cu SA/BCNx cannot significantly improve the catalytic activity and the Pt/Fe SA/BCNx reduce the selectivity towards glycine due to the formation of more acetic acid (AA) by-products. In contrast, Pd/Au/Ag/Co SA/BCNx catalysts achieve increased rates of glycine generation and maintain high selectivity with a small amount of GA product. Notably, the Pd SA/BCNx achieves the highest glycine generation rate of 2.9 mmol g -1 h-1 with a selectivity of 92%, cat surpassing all previously reported studies (Figure 3d and Table S3).\n19"}, {"round": 2, "reviewer_comments": {"reviewer_1": "The authors have addressed all the questions. The manuscript is ready for publication.", "reviewer_2": "All the concerns in my previous review have been well resolved in the revised manuscript. It is recommended to be accepted for publishing in Nature Communications.", "reviewer_3": "The authors are well improved the manuscript with optimal response. I suggest the publication in this form."}, "author_response": "Reviewer: 1\nThe authors have addressed all the questions. The manuscript is ready for publication.\nResponse: We thank the reviewer for the positive comments and recognition.\nReviewer: 2\nAll the concerns in my previous review have been well resolved in the revised manuscript. It is recommended to be accepted for publishing in Nature Communications.\nResponse: We thank the reviewer for the positive comments and recognition.\nReviewer: 3\nThe authors are well improved the manuscript with optimal response. I suggest the publication in this form.\nResponse: We thank the reviewer for the positive comments and recognition."}]

Decision letter