Carnegie stages

Carnegie stages
In embryology, Carnegie stages are a standardized system of 23 stages used to provide a unified developmental chronology of the vertebrate embryo.
The stages are delineated through the development of structures, not by size or the number of days of development, and so the chronology can vary between species, and to a certain extent between embryos. In the human being only the first 60 days of development are covered; at that point, the term embryo is usually replaced with the term fetus.
It was based on work by Streeter (1942) and O'Rahilly and Müller (1987). The name "Carnegie stages" comes from the Carnegie Institution of Washington.
While the Carnegie stages provide a universal system for staging and comparing the embryonic development of most vertebrates, other systems are occasionally used for the common model organisms in developmental biology, such as the Hamburger–Hamilton stages in the chick.
Stages
Days are approximate and reflect the days since the last ovulation before pregnancy ("Postovulatory age").
Stage 1: 1 days
fertilization
polar bodies
Carnegie stage 1 is the unicellular embryo. This stage is divided into three substages.
Stage 1 a
Primordial embryo. All the genetic material necessary for a new individual, along with some redundant chromosomes, are present within a single plasmalemma. Penetration of the fertilising sperm allows the oocyte to resume meiosis and the polar body is extruded.
Stage 1 b
Pronuclear embryo. Two separate haploid components are present - the maternal and paternal pronuclei. The pronuclei move towards each other and eventually compress their envelopes where they lie adjacent near the centre of the wall.
Stage 1 c
Syngamic embryo. The last phase of fertilisation. The pronuclear envelopes disappear and the parental chromosomes come together in a process called syngamy.
Stage 2: 2-3 days
cleavage
morula
compaction
Carnegie stage 2 begins when the zygote undergoes its first cell division, and ends when the blastocyst forms a cavity and comprises more than 16 cells. At this point, it is called a morula.
The cleavage divisions of CS2 embryos do not occur synchronously. And the fate of the blastomeres is not yet determined.
The two-cell embryo is spherical and surrounded by the transparent zona pellucida. Each of the blastomeres that form is also spherical.
On approximately day 3, at the eight-cell stage, compaction usually begins.
Stage 3: 4-5 days
blastocyst and blastocoele
trophoblast and embryoblast
Carnegie stage 3 begins when a cavity first appears in the morula and ends after the hatching from the zona pellucida when the embryo makes contact with the endometrial lining of the uterus.
There are only two stage 3 embryos in the Carnegie collection.
There are four characteristic processes that CS3 embryos go through cavitation, collapse and expansion, hatching, and discarding of cells.
Cavitation
The initiation of cavitation indicates the start of CS3. This process leads to the differentiation of blastocysts into outer trophoblast cells and inner embryoblasts.
Collapse and expansion
This process is seen in vitro and it is not known whether this occurs in vivo. In vitro, the blastocyst rapidly collapses and slowly re-expands before hatching from the zona pellucida.
Hatching
During this process, the blastocyst breaks through and escapes from the zona pellucida. This process must occur prior to implantation into the endometrium.
Discarding of cells
TEM inspection of in vitro blastocysts has allowed us to identify two types of cells that the developing embryo apparently discards. These are sequestered cells and isolated cells. Sequestered cells are groups of cells that are located in between the zona pellucida and the trophoblast. Isolated cells are mainly found in the blastocystic cavity.
Stage 4: 6 days
syncytiotrophoblast
cytotrophoblast
amniotic ectoderm
Stage 5 (a-c): 7-12 days
implantation
bilaminar embryonic disc
primary yolk sac
amniotic cavity
Stage 6: c. 17 days
primitive streak
primitive groove
chorionic villi
secondary yolk sac
early gastrulation
Stage 7: c. 19 days
gastrulation
neural plate
start of hematopoiesis
notochord
Stage 8: c. 23 days
primitive pit
Stage 9: c. 25 days
neural groove
neural folds
septum transversum
placode
early heart
Stage 10: c. 28 days
pharyngeal arches #1 and #2
cardiac loop
intermediate mesoderm
Stage 11: c. 29 days
sinus venosus
mesonephric duct
Stage 12: c. 30 days
upper limb buds
Stage 13: c. 32 days
septum primum
foramen primum
Stage 14: c. 33 days
ureteric bud
Stage 15: c. 36 days
Development of the Olfactory nerve and the early stage foot and hand plates
Stage 16: c. 39 days
lower limb buds
Stage 17: c. 41 days
implementation embryo in posterior uterus wall
Stage 18: c. 44 days
septum secundum
Stage 19: c. 46 days
Ectoderm: sensory placodes, lens pit, otocyst, nasal pits moved ventrally, fourth ventricle of brain
Mesoderm: heart prominence, ossification continues
Head: forebrain, eye, external acoustic meatus
Body: straightening of trunk, heart, liver, umbilical cord
Stage 20: c. 49 days
Ectoderm: sensory placodes, lens pit, otocyst, nasal pits moved ventrally, fourth ventricle of brain
Mesoderm: heart prominence, ossification continues
Head: forebrain, eye, external acoustic meatus
hearing - otic capsule connected with the basal plate and with the future exoccipitals. Tip of the cochlea is elongated and curled.
Tensor tympani and stapedius present.
Stage 21: c. 51days
Ectoderm: sensory placodes, nasal pits moved ventrally, fourth ventricle of brain
Mesoderm: heart prominence, ossification continues
Head: nose, eye, external acoustic meatus
Body: straightening of trunk, heart, liver, umbilical cord
Limb: upper limbs longer and bent at elbow, foot plate with digital rays begin to separate, wrist, hand plate with webbed digits
Stage 22: c. 53 days
Mesoderm: heart prominence, ossification continues
Head: nose, eye, external acoustic meatus
Body: straightening of trunk, heart, liver, umbilical cord
Limb: upper limbs longer and bent at elbow, foot plate with webbed digits, wrist, hand plate with separated digits
Stage 23: c. 56 days
final embryonic stage, after this development is described as "fetal" through the entire second and third trimester.
Mesoderm: ossification continues
Head: eyelids, external ears, rounded head
Body: straightening of trunk, intestines herniated at umbilicus
Limbs: hands and feet turned inward
See also
Mammalian embryogenesis
External links
UNSW Embryology - Carnegie Stages
Overview at umich.edu
Details on stages at lsuhsc.edu
List of structures by week, at mrc.ac.uk
Diagrams at actual size at Visible Embryo
Virtual human embryo detailing stages
References
Hill, M.A. (2016) Embryology Carnegie Stages. Retrieved August 19, 2016, from https://embryology.med.unsw.edu.au/embryology/index.php/Carnegie_Stages
Embryology