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http://peptide-soc.jp Vanderbilt AT1 19 Nature  Medicine 1 cDNA Ca² +  i cDNA Ca²+ ፣cDNA Ca 2+  1 Ca² + - - Ca² +  i Yale DNA DNA Shichiri M, Ishimaru S, Ota T, Nishikawa T, Isogai T, Hirata Y: Salusins: newly identified bioactive peptides with hemodynamic and mitogenic activities. Nat. Med., - ( ). , 2) DNA e-mail:  mshichiri.dgm@tmd.ac.jp DNA DNA 1) DNA s) 18 F RGD 5,6) RGD RGD RGD RGD 77 RGD DNA RGD DNA- ADA
DNA RGD 18F RGD PET  positron emission tomography 4) DNA- s) 4 DNA- 9) V-ATPase pH DNA DNA- DNA 9 , 10) DNA DNA 130 DNA 140 A DNA A A DNA Forskolin DNA PEG 11) DNA SV T KKKRK DNA DNA 120 Niidome T, Huang L, Gene therapy progress and pros- pects: Nonviral vectors, Gene Ther  , - ( ) Boussif O, Lezoualc'h F, Zanta MA, Mergny MD, Scher- man D, Demeneix B, Behr JP, A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine, Proc Natl Acad Sci USA ) - ( Pollard H, Remy JS, Loussouarn G, Demolombe S, Behr JP, Escande D., Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian  cells,  J  Biol  Chem - ( , Chen X, Park R, Tohme M, Shahinian AH, Bading JR, Conti PS, MicroPET and autoradiographic imaging of breast cancer alpha v-integrin expression using 18 F- . - and  61 Cu-labeled  RGD  peptide,  Bioconjug  Chem ( ) Kunath K, Merdan T, Hegener O, Haberlein H, Kissel T., Integrin targeting using RGD-PEI conjugates for in - ( vitro gene transfer, J Gene Med  , Harvie P, Dutzar B, Galbraith T, Cudmore S, O'Mahony D, Anklesaria P, Paul R., Targeting of lipid-protamine- DNA (LPD) lipopolyplexes using RGD motifs, J Lipo- some  Res , - ( ) Mizuguchi H, Koizumi N, Hosono T, Ishii-Watabe A, Uchida E, Utoguchi N, Watanabe Y, Hayakawa T, CAR- or alphav integrin-binding ablated adenovirus vectors, but not fiber-modified vectors containing RGD peptide, do not change the systemic gene transfer properties in ） mice, Gene Ther , ( - Ohmori N, Niidome T, Wada A, Hirayama T, Hatak- eyama T, Aoyagi H, The enhancing effect of anionic helical peptide on cationic peptide-mediating transfec- - tion systems, Biochem Biophys Res Commun ( ) McKenzie DL, Smiley E, Kwok KY, Rice KG, Low mo- lecular weight disulfide cross-linking peptides as nonvi- ral gene delivery carriers, Bioconjug Chem - ( McKenzie DL, Kwok KY, Rice KG. A potent new class of reductively activated peptide gene delivery agents, J Biol  Chem - ( ） Katayama Y, Fujii K, Ito E, Sakakihara S, Sonoda T, Murata M, Maeda M, Intracellular signal-responsive arti- ficial gene regulation for novel gene delivery, Biomacro- ) - ( molecules , 12. Zanta MA, Belguise-Valladier P, Behr JP, Gene deliv- ery: a single nuclear localization signal peptide is suffi- cient to carry DNA to the cell nucleus, Proc Natl - ) Acad Sci USA ( , Niidome T, Ohmori N, Ichinose A, Wada A, Mihara H, Hirayama T, Aoyagi H, Binding of cationic -helical peptides to plasmid DNA and their gene transfer abili- ties into cells, J Biol Chem - ( ) . Ohsaki M, Okuda T, Wada A, Hirayama T, Niidome T, Aoyagi H, In vitro gene transfection using dendritic poly (L-lysine), Bioconjug  Chem - . ( e-mail:  niidome-tcm@mbox.nc.kyushu-u.ac.jp URL: http://www.cstm.kyushu-u.ac.jp/katayama/ T
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T B 2) LPS 2,3) G -   - LPS 4） LPS × – 10 M LPS LPS C LPS 19 C LPS C 5） -   - Toll 6,7} ELISA LPS Toll LPS 1) 8） -  - Toll Toll Toll LPS 4） LPS -   - C B Toll C (9 Toll B 7 xo Toll tethered  ligand PAR tethered LPS LPS ligand PAR- LPS PAR- Ser-Leu-Ile-Gly-Arg-Leu G (11) μ M LPS Gly-Tyr-Pro-Gly-Lys-Phe PAR- LPS ATPase LPS G C C 1) LPS C G protease-activated G protein-coupled receptor, PAR 12, 130 PAR PAR PAR PAR- PAR- G 12 PAR- Arg 41 Arg 41–Ser–Phe–Leu–Leu–Arg–Asn– −Asp−Pro− Arg 41 Arg 41 Ala Ser PAR- μ M PAR- 14-17) Ser-Phe-Leu-Leu-Arg-Asn LPS PAR LPS LPS PAR PAR μ M PAR PAR LPS PAR C C 1) Ariki, S., Koori, K., Osaki, T., Motoyama, K., Inamori, K., and Kawabata, S.: A serine protease zymogen functions as a pattern-recognition receptor for lipopolysaccharides. Proc. Natl. Acad. Sci. USA, - ( ). , Hoffmann, J.A., Kafatos, F.C., and Janeway, C.A. Jr., and Ezekowitz, R.A.B.: Phylogenetic perspectives in innate immunity.  Science, - ( ). , Janeway, C.A. Jr. : Approaching the asymptote? Evolu- tion and revolution in immunology. Cold Spring Harbor Symp.  Quant.  Biol., , - ( ). Iwanaga, S., Kawabata, S., and Muta, T.: New types of clotting factors and defense molecules found in horse- shoe crab hemolymph: their structures and functions. , - J. Biochem. ). ( Osaki, T., Okino, N., Tokunaga, F. Iwanaga, S., and Kawabata, S.: Proline-rich cell surface antigens of horseshoe crab hemocytes are substrates for protein cross-linking with a clotting protein coagulin. J. Biol. Chem., - ( , ). Lemaitre, B., Nicolas, E., Michaut, L., Reichhart, J. M., and Hoffmann, J. A.: The dorsoventral regulatory gene cassette spaetzle/Toll/cactus controls the potent antifun- gal response in Drosophila adults. Cell, - , ). Medzhitov, R., Preston-Hurlburt, P., and Janeway, C.A. Jr.: A human homologue of the Drosophila Toll protein signals activation of adaptive immunity. Nature, - ( ). Anderson, K. V. and Nusslein-Volhard, C.: Information for the dorsal-ventral pattern of the Drosophila embryo is stored as maternal mRNA. Nature, ). ( Ligoxygakis, P., Pelte, N., Hoffmann, J.A., and Reichhart, J.M.: Activation of Drosophila Toll during fungal infec- tion by a blood serine protease. Science, - . ). ( Inamori, K., Ariki, S., and Kawabata, S.: A toll-like re- – ceptor in horseshoe crabs. Immunol. Rev., . ( ). Solon, E., Gupta, A.P., and Gaugler, R.: Signal transduc- tion during exocytosis in Limulus polyphemus granulo- cytes. Dev. Comp. Immunol.,   ,               , ( ). Vu, T.K., Hung, D.T., Wheaton, V.I., and Coughlin, S.R.: Molecular cloning of a functional thrombin receptor re- veals a novel proteolytic mechanism of receptor activa- - tion. Cell,  , ( ). Coughlin, S.R.: How the protease thrombin talks to cells. Proc. Natl. Acad. Sci. USA, ). ( Kawabata, S. and Tsuda, R.: Molecular basis of non-self recognition by the horseshoe crab tachylectins. Bio- - chim.  Biophys.  Acta, ( ). , Nagai, T. and Kawabata, S.: A link between blood co- agulation and prophenol oxidase activation in arthropod host defense. J. Biol. Chem., - ( ). , Nagai, T., Osaki, T., and Kawabata, S.: Functional con- version of hemocyanin to phenoloxidase by horseshoe crab antimicrobial peptides. J. Biol. Chem., , ( ). Osaki, T. and Kawabata, S.: Structure and function of coagulogen, a clottable protein in horseshoe crabs. ) in press. Cell. Mol. Life Sci.,   , ( e-mail: skawascb@mbox.nc.kyushu-u.ac.jp URL: www.biology.kyushu-u.ac.jp/~kawabata/ RIA RIA RIA PAGE RIA HPLC GRP RIA 1 } GRP RIA GRP SELDI MALDI ESI RIA MD ProGRP 2) Y. Miyake, et al. Pro-gastrin-releasing peptide ( - ) is a specific tumor marker in patients with small cell lung carcinoma.  Cancer Res.,   , K. Sasaki, et al.  Peptidomics-based approach reveals the secretion of the   -residue COOH-terminal frag- ment of the putative tumor suppressor protein DMBT from pancreatic adenocarcinoma cell lines. Cancer Res., - ( ） , e-mail:  ksasaki@ncc.go.jp  | non-RI Biofunctions, Computations, Analysis, Diseases, Hormones, Immunology, Pharmaceutics, Materials and nanosciences, Structure, Structure-activity relationships, Synthesis, Others. http://www.kyosemi.or.jp/ tamamura@pharm.kyoto-u.ac.jp rd International and The th European Peptide Symposium Prague, Czech Republic, September  – . http://www. eps.com/ FAX apipscc@mbox.nc.kyushu-u.ac.jp PEPTIDE NEWSLETTER JAPAN APIPS-JPS - - TEL&FAX E-mail: apipscc@mbox.nc.kyushu-u.ac.jp http://APIPS.scc.kyushu-u.ac.jp/ – TEL – FAX - e-mail:  hmihara@bio.titech.ac.jp - TEL - - – FAX e-mail: aotaka@pharm.kyoto-u.ac.jp TEL FAX - - - e-mail: kazuyasu@sci.hokudai.ac.jp TEL FAX - – e-mail:  iori@bse.kyutech.ac.jp - – TEL - - – FAX e-mail:  minamino@ri.ncvc.go.jp