Accelerate genomic
breakthroughs in microbiology.
Gain deeper insights with powerful bioinformatic tools.

Empowering
microbial genomics.
Extensive methods. Expansive possibilities.
In microbiome studies or environmental surveillance, high-resolution genomic data can be
instrumental in examining pathogenesis, gene transfer, pan-genomes, and coevolution of hosts
and parasites. Next-generation sequencing (NGS) enables many types of microbial studies,including direct evolution, mutation screening, and epidemiology. With the power and highresolution of Illumina NGS workflow solutions, you can now decipher the genetic makeup oforganisms that were previously impossible to study.

Powerful bioinformatics.
Intuitive tools provide critical insights.
Easily translate your genomic variant data into valuable discoveries with our push-button,
cloud-based bioinformatics solutions. Securely transfer, store, analyze, and share your microbial
data in the BaseSpace® Cloud or BaseSpace Onsite, the local version of the platform.

For Research Use Only. Not for use in diagnostic procedures.

A breadth of applications.
A depth of discoveries.
Multiple microbiology applicationsare accessible with the MiSeq® System.

For Research Use Only. Not for use in diagnostic procedures.

Human healthWith the advent of next-generation sequencing, a new field of research, metagenomics, was born. Enabling
comprehensive examination of microbial communities without cultures, metagenomics allows researchers toanalyze the impact of each person’s unique microbiome.

Reproductive health
Assess imbalances in bacterial communities in the vagina that increase the risk for infertility,
spontaneous abortion, and preterm birth. Organizations like The Vaginal Microbiome
Consortium are studying the impact of the vaginal microbiome on women’s health.

Agriculture
Analyze the equilibrium and symbiosis of microbes and plants. The EarthMicrobiome Project is currently focusing on a systematic attempt to construct
the Earth’s microbial biomap.

BioPharma
Monitor and possibly identify bacteria involved in risk monitoring. A team ofscientists are using NGS technology for cell-line genetic stability testing andbiosafety in-process monitoring.

Population
Understand human microbial variation and corresponding genetic parameters.
The American Gut Project is using a 16S rRNA gene sequencing solution tostudy human gut diversity.

Environment
Examine a specific bacterial gene from a sample microbial community with a complex andrapidly changing ecosystem. Chris Mason and his team at Cornell School of Medicine are
creating a comprehensive map to study the microbiome and metagenome of a city’s surfaces.

Infectious disease
Discover generalized mechanisms of pathogen transmission and evolution. “There’s definitely a strong clinicalneed for fast NGS data analysis, especially in the areas of pathogen detection and infectious disease,” said
Charles Chiu, Director of the Clinical Microbiology Laboratory at the University of California, San Francisco.

Go where thescience takes you.
Amplify your research with our 16S workflow solution.
Take your 16S rRNA studies to the next level. Clarify phylogenetic relationships between bacteria, andidentify differences between healthy and disease states. Streamline rRNA sequencing of skin, stool, orblood samples, and optimize individual organism analysis. From library prep to analysis, our seamless
NGS workflow simplifies the process.

Obtain high-resolution 16S phylogeny
16S rRNA sequencing

Entire workflow fully supported by Illumina

Sample Prep

V3-V4 Region
Amplification

Meta-G-Nome™
DNA Isolation Kit

16S library
prep protocol

Prepare Library

Sequence

Nextera® XT Kit

MiSeq SystemAnalyze Data &Annotate Variants

Cloud—BaseSpace Platform
Local—MiSeq Reporter
16S Metagenomics

For Research Use Only. Not for use in diagnostic procedures.

Shotgun metagenomics
Explore all genes in all organisms present in many complex environmental
or microbial samples. Identify symbiosis of organisms within an environment.
Evaluate bacterial diversity and detect microbe abundance. Study even themost challenging microorganisms.

Whole-genome sequencing
Sequence the entire genome and achieve accurate de novo assembly using
cultures as starting material. Generate reliable reference genomes and sequencepreviously challenging microbes. Compare genomic studies and identify drug
resistance relationships with simplicity and ease.

Virology
Detect low-frequency viral particles within a noncultured sample. Identify,
characterize, and quantify DNA and RNA viral transcripts of infectious agentswith a hypothesis-free approach. Rapidly isolate a gene or mutation associatedwith drug resistance. Identify quasi-species or coinfections within a sample.

VidEntia our complete
Qui
Optimize withInvel

NGS workflow solutions.
One seamless process. One complete resource.
From library prep to sequencing and informatics, Illumina offers comprehensive NGS workflow
solutions that optimize your process and accelerate your progress. As the innovators of next-generation
technology, we’re here to deliver the experience and expertise to help advance your microbiology research.

World-class solutions.
A community of support.
From sample prep, library prep, arrays, and sequencing to informatics, Illumina
next-generation solutions empower researchers and clinical researchers acrossthe globe to find the answers they seek.
When you join the Illumina community, you become part of a dynamic scientificmovement that includes thousands of researchers and industry thought leaders.
Throughout the year, we host user group meetings, symposia, consortia, online
forums, and other initiatives—all designed to bring the best minds together toshare ideas and advance science.
In addition to on-site training, ongoing support, and phone consults, we offer
webinars and courses at various Illumina locations. We’re here with all theresources you need to accelerate progress.

Discover more about our microbiology solutions at
www.illumina.com/microbiology.
A global genomics leader, Illumina provides complete next-generation sequencing workflow solutions to thebasic and translational research communities. Illumina technology is responsible for generating more than
90% of the world’s sequencing data. Through collaborative innovation, Illumina is fueling groundbreaking
advancements in the fields of oncology, reproductive health, genetic disease, microbiology, agriculture,and forensic science.
Illumina • 1.800.809.4566 toll-free (US) • +1.858.202.4566 tel • techsupport@illumina.com • www.illumina.com
For Research Use Only. Not for use in diagnostic procedures.
© 2015 Illumina, Inc. All rights reserved. Illumina, BaseSpace, MiSeq, Nextera, and the pumpkin orange color are trademarks of Illumina, Inc.
and/or its affiliate(s) in the US and/or other countries. All other names, logos, and other trademarks are the property of their respective owners.
Pub. No. 1270-2014-005 Current as of 27 July 2015