Leica VT1000 S
Vibrating-blade microtome
Instructions for Use
EnglishOrder No.: 14 0472 80101 - Revision O

Version 2.6, Revision O - 10.2023

Always keep this manual with the instrument.
Read carefully before working with the instrument.

Note
The information, numerical data, notes and value judgments contained in this Instructions for Use
represent the current state of scientific knowledge and state-of-the-art technology as we understand it
following thorough investigation in this field.
We are under no obligation to update the present Instructions for Use periodically and on an ongoingbasis according to the latest technical developments, nor to provide our customers with additional
copies, updates etc. of this Instructions for Use.
To the extent permitted in accordance with the national legal system as applicable in each individual
case, we shall not be held liable for erroneous statements, drawings, technical illustrations etc.
contained in this Instructions for Use. In particular, no liability whatsoever is accepted for any financial
loss or consequential damage caused by or related to compliance with statements or other informationin this Instructions for Use.
Statements, drawings, illustrations and other information regarding the contents or technical details of
the present Instructions for Use are not to be considered warranted characteristics of our products.
These are determined only by the contract provisions agreed between ourselves and our customers.
Leica reserves the right to change technical specifications as well as manufacturing processes without
prior notice. Only in this way is it possible to continuously improve the technology and manufacturing
techniques used in our products.
This document is protected under copyright laws. All copyrights to this documentation are held by Leica
Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including any electronic systems and media – requires expressprior permission in writing by Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of manufacture, please refer to the nameplate on the back of
the instrument.

Leica Biosystems Nussloch GmbH
Heidelberger Strasse 17 - 1969226 Nussloch
GermanyTel.:+49 - (0) 6224 - 143 0
Fax:+49 - (0) 6224 - 143 268
Web:
www.LeicaBiosystems.com
Assembly contracted to Leica Microsystems Ltd. Shanghai
Leica VT1000 S

3

Table of contents
1.

Important Information................................................................................................................................. 5
1.1
1.2
1.3
1.4
1.5

2.

Safety........................................................................................................................................................... 10
2.1
2.2
2.3

3.

Technical data................................................................................................................................................. 12
General overview - Leica VT1000 S............................................................................................................. 14

Installation.................................................................................................................................................. 16
4.1
4.2

5.

Safety notes..................................................................................................................................................... 10
Warnings.......................................................................................................................................................... 10
Safety instructions for handling the instrument........................................................................................ 11

Instrument Characteristics ..................................................................................................................... 12
3.1
3.2

4.

Symbols and their meanings.......................................................................................................................... 5
Instrument model.............................................................................................................................................. 8
Information......................................................................................................................................................... 8
Qualification of personnel............................................................................................................................... 8
Intended use/improper use............................................................................................................................. 9Standard delivery............................................................................................................................................ 16
Unpacking and setting up the instrument.................................................................................................. 17

Operation .................................................................................................................................................... 20
5.1
5.2
5.3
5.4
5.5
5.6

Installation site requirements....................................................................................................................... 20
Setting up the instrument.............................................................................................................................. 20
The operating elements and their function - Leica VT1000 S................................................................. 22
Adjusting the amplitude................................................................................................................................. 26
Working with the Leica VT1000 S on a daily basis.................................................................................... 27
Routine daily maintenance and switching off the instrument - Leica VT1000 S.................................. 30

6.

Malfunctions: Meaning and Troubleshooting...................................................................................... 31

7.

Cleaning and Maintenance ..................................................................................................................... 35
7.1
7.2

8.

Cleaning the instrument................................................................................................................................ 35
Changing the fuse........................................................................................................................................... 36

Ordering Information: Spare Parts, Accessory, Consumables.......................................................... 37
8.1
8.2
8.3
8.3.1
8.4
8.5

Ordering information...................................................................................................................................... 37
Foot switch....................................................................................................................................................... 37
Buffer tray........................................................................................................................................................ 38
Double-walled buffer tray S.......................................................................................................................... 38
Magnifier, LED illumination........................................................................................................................... 39
Julabo recirculating cooler/chiller FL300................................................................................................... 40

9.

Warranty and Service............................................................................................................................... 41

10.

Decontamination Confirmation............................................................................................................... 42

4

Version 2.6, Revision O

Important Information
1.

Important Information

1.1

Symbols and their meanings

1

CautionLeica Biosystems GmbH assumes no liability for consequential loss or damage due to failure toobserve the following instructions, particularly in relation to transportation and package handling,
and failure to observe the instructions for handling the instrument carefully.
Symbol:Title of the symbol:
Description:

Warning
If this danger is not avoided, then this may result indeath or serious injury.

Symbol:Title of the symbol:
Description:

CautionIndicates a potentially hazardous situation which, if
not avoided, could result in death or serious injury.

Symbol:Title of the symbol:
Description:

Note
Indicates a situation with the potential for property
damage which, if not avoided, could result in damage
to the machine or something in its vicinity.

Symbol:→ "Fig. 7 - 1"

Title of the symbol:
Description:

Symbol:Start

Title of the symbol:
Description:

Symbol:Title of the symbol:
Description:

Symbol:Title of the symbol:
Description:

Symbol:Title of the symbol:
Description:

Item number
Item numbers for numbering illustrations. Numbers in
red refer to item numbers in illustrations.
Function key
Software symbols that have to be pressed on
the input screen are displayed as bold, gray andunderlined text.
China ROHS
Environmental protection symbol of the China RoHS
directive. The number in the symbol indicates the"Environment-friendly Use Period" of the product inyears. The symbol is used if a substance restricted
in China is used in excess of the maximum permitted
limit.
WEEE symbol
The WEEE symbol, indicating separate collection
for WEEE - Waste of electrical and electronicequipment, consists of the crossed-out wheeled bin(§ 7 ElektroG).
Manufacturer
Indicates the manufacturer of the product.

Leica VT1000 S

5

1 Important Information
Symbol:Title of the symbol:
Description:

Manufacturing date
Indicates the date when the device was
manufactured.

Symbol:Title of the symbol:
Description:

Symbol:Title of the symbol:
Description:

Symbol:Title of the symbol:

CE Compliance
The CE marking is the manufacturer's declarationthat the product meets the requirements of theapplicable EC directives and regulations.
UKCA Label
The UKCA (UK Conformity Assessed) marking is
a new UK product marking that is used for goods
being placed on the market in Great Britain (England,
Wales and Scotland). It covers most goods which
previously required the CE marking.
UK Responsible Person

Description:

The UK Responsible Person acts on behalf of the
non-UK manufacturer to carry out specified tasks inrelation to the manufacturer’s obligations.
Country of Origin
The Country of Origin box defines the country where
the final character transformation of the product has
been performed.
Observe the Instructions for Use
Indicates the need for the user to consult the
instructions for use.

Symbol:Title of the symbol:
Description:

Symbol:Title of the symbol:
Description:

Symbol:Title of the symbol:
Description:

Order No.
Indicates the manufacturer's catalog number so thatthe device can be identified.

Symbol:Title of the symbol:
Description:

Serial number
Indicates the manufacturer's serial number so that a
specific device can be identified.

Symbol:Title of the symbol:
Description:

Fragile, handle with care
Indicates a device that can be broken or damaged if
not handled carefully.

Symbol:Title of the symbol:
Description:

Store dry
Indicates a device that needs to be protected frommoisture.

6

Version 2.6, Revision O

Important Information

1

Symbol:Title of the symbol:
Description:

This way up
Indicates correct upright position of the transport
package.

Symbol:Title of the symbol:
Description:

Stack limit
The largest number of identical packages allowed to
be stacked; "2" stands for the number of permitted
packages.

Symbol:Title of the symbol:

Temperature limit for transport

Description:

Indicates the temperature limits for transport towhich the device can be safely exposed.

Title of the symbol:

Temperature limit for storage

Description:

Indicates the temperature limits for storage to which
the device can be safely exposed.

Symbol:Title of the symbol:
Description:

Humidity limitation for transport and storage
Indicates the range of humidity for transport andstorage to which the device can be safely exposed.

Symbol:Title of the symbol:
Description:

Tilt indicator
Tip-n-Tell indicator to monitor whether the shipment
has been transported and stored in upright position
according to your requirements. With a pitch of
60° or more, the blue quartz sand flows into thearrow-shaped indicator window and sticks there
permanently.

Symbol:Improper handling of the shipment is immediately
detectable and can be proven definitively.

Leica VT1000 S

7

1 Important Information
Symbol:Title of the symbol:
Description:

Shockdot Impact Indicator
In the Shockwatch system, a shock dot shows
shocks or impacts that are above a specified
intensity through red coloration. Exceeding a defined
acceleration (g value) causes the indicator tube tochange color.

Symbol:Title of the symbol:
Description:

Recycling
Indicates, that the item can be recycled where
correct facilities exist.

Symbol:Title of the symbol:
Description:

Regulatory Compliance Mark (RCM)
The Regulatory Compliance Mark (RCM) indicates adevice’s compliance with applicable ACMA technicalstandards of New Zealand and Australia - that is, fortelecommunications, radio communications, EMC
and EME.

1.2

Instrument model
All information provided in these Instructions for Use applies only to the instrument type indicated on
the title page. A nameplate indicating the instrument serial number is attached to the rear side of the
instrument.

1.3

Information
When making inquiries, please specify correctly:• Instrument model• Serial number

1.4

Qualification of personnel
The Leica VT1000 S should be operated by trained laboratory personnel only. The instrument is intended
for professional use only.
All laboratory personnel designated to operate this instrument must read these Instructions for Use
carefully and must be familiar with all technical features of the instrument before attempting to operateit.

8Version 2.6, Revision O

Important Information
1.5

1Intended use/improper use
The Leica VT1000 S is used for sectioning in the fields of medicine, biology and industry, and is
especially designed for sectioning fixed or unfixed fresh tissue in a buffer solution.
Warning
The Leica VT1000 S may be used for research purposes only. Sections made using the Leica VT1000 S
must NOT be used for diagnostics!
The instrument must be used exclusively according to the instructions contained in these Instructions
for Use.
Any other use of the instrument is considered improper.

Leica VT1000 S

92 Safety
2.

SafetyWarning
The safety and caution notes in this chapter must be observed at all times.
Be sure to read these notes even if you are already familiar with the operation and use of other Leica
products.

2.1

Safety notes
These Instructions for Use includes important information related to the operating safety andmaintenance of the instrument.
The Operating Manual is an important part of the product, and must be read carefully prior to startup
and use and must always be kept near the instrument.
This instrument has been built and tested in accordance with the safety requirements for electricalequipment for measurement, control, and laboratory use.
To maintain this condition and ensure safe operation, the user must observe all notes and warnings
contained in these Instructions for Use.
The current EC Declarations of Conformity and UKCA Declaration of Conformity can be found on theInternet: www.LeicaBiosystems.com
Note
These Instructions for Use must be appropriately supplemented as required by the existing
regulations on accident prevention and environmental safety in the operator‘s country.
Warning
The protective devices located on the instrument and the accessories must not be removed or
modified. The instrument must only be opened and repaired by service technicians authorized by
Leica.

2.2

Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis for accident
prevention. Operating the instrument safely is, above all, the responsibility of the owner, as well as thedesignated personnel who operate, service or clean the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following instructions
and warnings.

10

Version 2.6, Revision O

Safety
2.3

2

Safety instructions for handling the instrument
DangerCaution
Extremely sharp blades pose risk of injury when touched!
CautionFresh tissue poses risk of infection!
CautionWhen not in use, cover magnifier with corresponding lid to avoid risk of fire.
Warning
Avoid touching live parts under any circumstances!
Proper handling• Be sure to handle knives and blades very cautiously!
• Never touch the cutting edge of knives and blades!
• Do not leave knives, blades and bladed knife holders unprotected.
• All appropriate safety precautions must be met to avoid the risk of infection.
• Protective clothing according to safety regulations for "Working with harmful substances" (Safetymask, gloves, protective clothing) must be worn!
• Cover the magnifier during work breaks as it may act as a burning glass when not covered!
• In case of emergency, press the red EMERGENCY STOP switch (at the right side of the instrument).
To release the switch, turn it in the direction of the arrow.
• The instrument may be opened by authorized service personnel only.
• Before removing the cover, ensure that the instrument is unplugged.

Leica VT1000 S

11

3 Instrument Characteristics
3.

Instrument Characteristics

3.1

Technical data
Electrical Specifications
Nominal supply voltage
Nominal supply frequencies
Mains supply voltage fluctuations
Power consumption
Mains input fuses
Dimensions and Weight Specification
Overall size of device in operating mode
(Width x Depth x Height, mm)
Overall size serial packaging(Width x Depth x Height, mm)
Empty weight (without accessories, kg)
Overall weight (with accessories, kg)
Device weight including packaging (kg)
Enviromental Specification
Operating altitude (meters)
Temperature (operation) (min/max)
Relative humidity (operation) (min/max)
Temperature (transit) (min/max)
Temperature (storage) (min/max)
Relative humidity (transit/storage)
Operating noise level
Emissions and Boundary Conditions
Overvoltage category to IEC 61010-1
Pollution degree to IEC 61010-1
Means of protection to IEC 61010-1
Degree of protection to IEC 60529
Heat emission
A-weighted noise level, measured at 1 m distance
EMC classElectrical Connections and Interfaces
Power supply
Electrical Connections
Mechanical Connections
Interfaces to other devices

12

100 V - 240 V
50/60 Hz
Not to exceed ± 10 % of the nominal supply
voltage
50 VA
2x T1.25A L 250VAC
480 mm x 360 mm x 200 mm
780 mm x 585 mm x 656 mm
17 kg
19 kg
40 kg
up to 2000 m above sea levelmin. +18 °C to max. +30 °C
max. 80 %-29 °C to +50 °C+5 to +50 °C
10 %-85 %rh<70 dB
II
2Class I
IP20
50J/s
<70 dB
B
Connecting socket for power cord
Connection for foot switch, 9-pole
Fixture for magnifier support, Knife holder,
Buffer tray S, Magnifier support, Magnifier,
Module Hi-Power spot, LED 1000 (optional),
Module LED Hi-Power spots, 2-arm (optional)

Version 2.6, Revision O

Instrument Characteristics
Other Specifications
Sectioning frequency (± 10 %)Amplitude
Sectioning range
Specimen orientation, rotating
Electrical overload protection
Internal current limit for the electronics
Height with magnifier support
Magnifier support
CE
Sectioning speed (± 10 %)Return stroke speed (± 10 %)Total vertical specimen stroke
Maximum specimen size: with standard knife holder
Section thickness selection
Magnifier, assy. (standard accessory of theconfigured instrument)

Leica VT1000 S

3

0 - 100 Hz
adjustable in 5 steps: 0.2; 0.4; 0.6; 0.8; 1 mm
1 - 40 mm (adjustable)
330°, 0 - 999 μm (adjustable; can be deactivated)
Yes
Yes
285 mm2 kg
Yes
0.025 - 2.5 mm/s
5 mm/s15 mm (motorized)
33 x 40 mm
1 - 999 μm, in 1 μm steps
2 x magnification13

3 Instrument Characteristics
3.2

General overview - Leica VT1000 S

7
8

1

9

3

10

4
5
2

6

Fig. 1

Fixture for magnifier support
Control panel
EMERGENCY STOP switch (not visible here)
Cutting head
Knife holder

1
2
3
4
5

6
7
8
9
10

2

Buffer tray S
Magnifier support
Magnifier
Module Hi-Power spot, LED 1000 (optional)
Module LED Hi-Power spots, 2-arm(optional)

3

4

1

5
Fig. 21
2

14

EMERGENCY STOP button
Fixing device for drain tube

3
4
5Connecting socket for power cord
Power switch
Connection for foot switch, 9-pole

Version 2.6, Revision O

3

Instrument Characteristics

1

8

2

9

3

10
11

4

12
13

5

14

6

15
7

Fig. 3

1
2

Rotary knob for sectioning speed
Button for maximum advance speed

893

Indication of selected section thickness or
totalized section thickness in μm+/- selection button for section thickness (1 999 μm selectable), retraction and/or volume
Button for setting the limit stops of thesectioning window
Start button for single / continuous sectioning
strokePause button - stops sectioning process

10

Rotary knob for sectioning frequency
LED mode indication: "Totalized section
thickness"
LED mode indication: "Section thickness"

11

CLR-Clear button

12

DISP-Programming button

13

Selector button "Single/continuous stroke"(LEDs indicate selected mode)
Toggle switch "Buffer tray heightadjustment" (LEDs indicate limit positions)
Toggle switch for knife forward and returnstroke

4
5
6
7

14
15

Leica VT1000 S

15

4 Installation
4.

Installation

4.1

Standard delivery
Qty
1
1
1

1
1

Designation

Order No.

Basic instrument
Silicon tube
set of replacement fuses 2 x T 1.25 A
toolset:1
Hexagon key, No. 2.5
1
Hexagon key, No. 8.0
1
Manipulator
Microtome protective coverInternational bundle Instructions for Use (incl. English printout and additional
languages on a data storage device 14 0472 80200)
Leica VT1000 S complete configuration
Leica VT1000 S basic instrument
Specimen discs S, non orientable
Buffer tray S
5Countersunk screw, M 5 x 8
2
Hose clamps
Knife holder S – for injector and razor blades
Hexagon key w/handle, size 3
1
Bottle of Cyanoacrylate adhesive
Magnifier assy. (magnifier glass & carrier)

14 0472 35612
14 0462 27513
14 6000 04803
14 0194 13195
14 0222 04143
14 0462 28930
14 0212 04091
14 0472 80001
14 0472 35613
14 0472 35612
14 0463 27404
14 0462 30132
14 2101 77121
14 0481 41952
14 0462 30131
14 0194 58333
14 0371 27414
14 0462 31191

If the supplied local power cord is defective or lost, please contact your local Leica Biosystems
representative.
Note
When ordering additional accessories, compare the parts received with the parts ordered. If theparts received do not match your order, contact the sales company responsible for your order
immediately.

16

Version 2.6, Revision O

Installation
4.2

4

Unpacking and setting up the instrument
Note
The packaging has two indicators, ShockDot Impact Indicator and Tilt Indicator, that indicate
improper transport. When the instrument is delivered, check these first. If one of the indicators
is triggered, the package was not handled as prescribed. In this case, please mark the shipping
documents accordingly and check the shipment for damage.
1• Remove the packing strap and the adhesive• tape (→ Fig. 4‑1).
• Remove the carton lid (→ Fig. 4‑2).
• Take out the accessory carton (→ Fig. 4‑3).
• Take Hexagon key No. 8 out of the accessory
carton and put it aside for later use.
• Remove the fixing carton (→ Fig. 4‑4).
• Remove the outer carton wall (→ Fig. 4‑5).
• Lift the instrument out of the carton by the
carrying straps (→ Fig. 4‑6) and place it on asuitable stable laboratory table.

2

3
4

5

6

Fig. 4

Leica VT1000 S

17

4 Installation
2
3
1• Tilt the instrument including the baseplate(→ Fig. 5) - hold the instrument with one
hand on the recess (→ Fig. 5‑2) for the buffer
tray! NEVER lift or hold it by the cutting head(→ Fig. 5‑3)! Unscrew the screw (→ Fig. 5‑1)
using the size 8 Hexagon key provided andremove the base plate.

Fig. 5• Using both hands at the sides (→ Fig. 6), grasp
the bottom of the instrument and carefully place
it on a suitable laboratory table.

Fig. 6

Note
Compare with the attached packing list to make sure the delivery is complete.
Note
The transport carton and included retaining elements should be kept in case a return shipment isnecessary later. To return the instrument, follow the instructions above in reverse order.

Assembling the drain tube
• Bottom of the instrument (→ Fig. 7).
• Connect the drain tube (→ Fig. 7) to the bottom of the instrument (→ Fig. 7‑1).
• Ensure that the loose end of the drain tube is closed tightly with the matching stopper.
• Secure the loose end of the drain tube in the holder at the rear of the instrument (→ Fig. 7‑2).

18

Version 2.6, Revision O

Installation

4

1

2

Fig. 7

Assembling the magnifier support and foot switch (optional)• The magnifier support (→ Fig. 8‑1) is packaged separately.
• Set it on the instrument as shown in (→ Fig. 8).
• Attach the optional foot switch.
• Securely plug the foot switch into the 9-pin socket (→ Fig. 8‑2).

12
Fig. 8

Note
When transporting the instrument, always do so WITHOUT the magnifier support!

Leica VT1000 S

19

5 Operation
5.

Operation

5.1

Installation site requirements
The place of installation must meet the following requirements:• The instrument is designed for indoor use only.
• The power plug must be freely and easily accessible.
• Power supply at a distance no greater than the length of the power cable (3m) – an extension cablemust not be used.
• Level installation location.
• Substrate as free of vibration as possible.
• Relative humidity should not exceed 80 %.
• Room temperature consistently between +18 °C to +30 °C.
• Avoid vibrations, direct sunlight, and large temperature fluctuations!
Warning
The instrument may not be operated in hazardous locations.
Warning
The instrument MUST be connected to a grounded power socket. Use only a provided power cablethat is intended for the local power supply.

5.2

Setting up the instrument
Note
The instrument MUST be connected to a grounded power socket. Use only a provided power cablethat is intended for the local power supply.
Warning
The instrument MUST be set up so that the power plug and switch are free and easily accessible at
all times!
Note
The Leica VT1000 S is equipped with a autoranging power supply to cover voltages from
100 V to 240 V.
After switching on the main switch, the instrument carries out an initial startup run: The blade returns
to the rear starting position after a short forward movement.
1. Put the main switch at the back of the instrument to the OFF position.
2. Make sure the power cable is connected correctly to the instrument.
3. Attach the magnifier support.
4. Insert the buffer tray.

20

Version 2.6, Revision O

Operation

5

5. Insert the knife holder.
6. Insert a blade into the knife holder.
7. Connect the magnifier support with Module LED Hi-Power spots, 2-arm, as shown in (→ Fig. 9).
Insert plug (→ Fig. 9‑1) of the Module LED Hi-Power spots, 2-arm, into socket (→ Fig. 9‑2) atthe Module Hi-Power spot, LED 1000.

1

2

Fig. 98. Connect the optional foot switch at the rear of the instrument.
9. Plug the power cable into the wall socket.
10. Switch the instrument on (main switch).

Leica VT1000 S

21

5 Operation
5.3

The operating elements and their function - Leica VT1000 S
CautionPractice working with the controls without a blade holder inserted. Only insert the knife holder when
you are completely familiar with all control functions.
1Fig. 10

Fig. 1122

SPEED
Scale setting
0

mm/s
0.00

0.5

0.025

1

0.050

2

0.075

3

0.125

4

0.175

5

0.225

6

0.40

7

0.65

8

0.90

9

1.30

10

2.50

FREQ
Scale setting
0

Hz
0

0.5

8

1

10

2

20

3

30

4

40

5

50

6

60

7

70

8

80

9

90

10

100

10-speed rotating potentiometer with scale
Function:
Continuous knife feed adjustment from0.05 - 2.5 mm/s:
Knife return stroke is performed at constant
speed of 5 mm/s.
The additional locking lever (→ Fig. 10‑1)(lever in 12 o'clock position) prevents the
speed setting from being accidentally changed
while sectioning is in progress.

Rotary knob with scale from 0 to 10
Function:
Continuous adjustment of knife sectioning
frequency (vibration) from 8 - 100 Hz.

Version 2.6, Revision O

Operation

5

Function:

Button with LED• When the V-Max button is activated in manual mode (LED on red light) and the REV/FORW button is pressed, the knife moves
towards the specimen at maximum speed.
• When the START button is pressed, the LED in the V-Max
button is extinguished. Sectioning starts at the speed previously
selected.

Setting a sectioning window:
Warning
If - accidentally - only one limit stop of the sectioning window is set, the knife covers the maximum
sectioning range!

Button with LED• Activate the V-Max button. Press REV/FORW toggle switch forfast movement of the blade towards the specimen. Press the
button to set the first limit of the sectioning window.
• Press REV/FORW once again, moving the blade edge past the
specimen block and press once more to set the second
sectioning window limit.
• Press START to deactivate V-Max. The knife edge moves back to
the first sectioning window limit and resumes sectioning at thepreviously selected speed (10-speed rotary potentiometer).
Function:• Start single or continuous sectioning stroke – according towhether SINGLE or CONT mode has previously been selected(→ p. 24 – LED indication with -/+ adjusting button, DISP and
CLR function keys).
• Specimen feed (section thickness) takes place prior to eachsection.
• Retraction (specimen is lowered) takes place when the knife
reaches the rear inversive point.
• In SINGLE mode, the knife stops automatically in the rear end
position.
• In CONT mode, START/STOP has to be pressed again to stop thesectioning movement. The knife stops in the rear end position.
• A sectioning process, once started, will continue.
Function:
Immediate interruption of knife movement.
• Press PAUSE once again to continue sectioning.

Leica VT1000 S

23

5 Operation
Toggle switch
Function:
To move the knife towards the specimen.
Fig. 12

Can also be used for manual sectioning.
Because of safety aspects the FORW movement is carried out only
while the toggle switch is pressed and held; the REV movement iscarried out completely once the switch has been locked into place.
To stop the REV movement before reaching the rear end position,
switch the toggle switch manually back into its center position.
The REV/FORW switch can also be used to stop a sectioning stroke
which has been activated by pressing the START/STOP button.
LED indication with -/+ adjusting button, DISP and CLR function
keys

Fig. 13

Function of LED indication:
Indicates the selected sectioning thickness or totalized section
thickness.
Function of the -/+ button:
Selection of section thickness in 1-μm steps from 0 to 999 μm.
The specimen feed (in the preselected section thickness) takes
place at the beginning of each sectioning stroke.
Function of the DISP button:
To select between two modes of operation:"Σμm"= section thickness totalizing"μm" = section thickness
Function of the CLR button in section thickness totalizing mode:
Sets the value indicated in the section thickness totalizing mode(Σμm) to zero.

24

Version 2.6, Revision O

Operation

5

Function:
Switch between
Button with LED• Single stroke (1 sectioning stroke / 1 return stroke of the blade)and continuous stroke (continuous sectioning until the START/STOP button is pressed).
• To stop the blade at the rear end position in CONT mode press
the START/STOP button.
• The sectioning stroke in progress will be completed and theblade will then stop at the selected end position of the sectioning
range.
Toggle switch
Function:• Motorized height adjustment of buffer tray. Maximum travel: 15mm (= total vertical specimen stroke).

Fig. 14

The upper and lower end positions of the buffer tray are
indicated each by an audible warning signal and a red LED.
While the knife is in motion the UP/DOWN toggle switch is
inoperational.
For the DOWN motion, the toggle switch can be locked in the
DOWN position; For the UP motion, the switch must be pressedand held in the UP position.
When the lowest possible position is reached with the toggle
switch being locked in DOWN there will be both an audible anda visible signal. Once the switch is unlocked, the buffer tray is
automatically raised until both signals switch off.
• To select the retraction thickness, to deactivate retraction or to
set the volume of the Leica VT1000 S warning signal, press thefollowing function key combinations:
Volume adjustment:

Fig. 15

Leica VT1000 S• Select section thickness mode ("μm") by pressing the DISP
button.
• Press the CLR and + buttons simultaneously. Display: "BE 15".
The volume can now be adjusted via the -/+ button.
"0" is equivalent to no sound signal.
• To quit the programming mode, press CLR.

25

5 Operation
Adjusting the retraction• In programming mode, press DISP to display the specimen
retraction menu.
• Display: "LO".
• Set specimen retraction between 1 and 999 μm via the -/+ button;
or disable by selecting "0".
• The selected value will be displayed in the FEED window.
• Press CLR to quit the menu function.

Fig. 16

5.4

Adjusting the amplitude• To obtain excellent sectioning results, the amplitude requires
adjustment according to the specimen type being sectioned.
1

To this end:
• With a No. 2.5 Hexagon key loosen the clamping screw(→ Fig. 17‑1) and secure the eccentric on the bottom with yourfinger.
Selectable amplitude positions are, from left to right:
0.2 mm; 0.4 mm; 0.6 mm; 0.8 mm; 1 mm.
• Slide the amplitude clamping screw to the desired amplitude
position and retighten.

Fig. 17

Note
When adjusting the amplitude setting, do not remove the clamping screw, simply loosen it.
The instrument is shipped with the amplitude set to 0.6 mm.

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Operation
5.5

5

Working with the Leica VT1000 S on a daily basis
1
4
2
3

Fig. 18• Mount the buffer tray (→ Fig. 18‑1) onto the bolt (→ Fig. 18‑2)
inside the cooling bath (→ Fig. 18‑3).
• Secure the buffer tray by relocating the clamping lever(→ Fig. 18‑4) to the right (in the direction of the arrow).
• Via the UP/DOWN toggle switch lower the buffer tray to its
lowest position (indicated by audible signal and red LED).
• Move the toggle switch back to the mid-position - the audible
signal stops.
• If necessary, fill crushed ice into the cooling bath (→ Fig. 18‑3).
• Fill the buffer tray (→ Fig. 18‑1) with cooled buffer solution.
• Fix the specimen onto the specimen disc with cyanoacrylateadhesive (→ Fig. 19).

Fig. 192• Insert the specimen disc (→ Fig. 20‑1) with the specimen into thebuffer tray using the manipulator (→ Fig. 20‑2).

1

Fig. 20

Leica VT1000 S

27

5 Operation
2
1• Use the manipulator (→ Fig. 21‑2) to rotate the specimen disc
into the desired position. Tighten with a No. 3 Hexagon key(→ Fig. 21‑1).
• The clamping screw or one of the clamping devices must not belocated over the gap in the specimen disc, as in these positions
clamping the specimen disc is not possible.
• Remove the manipulator (→ Fig. 21‑2).

Fig. 21

Adjusting the clearance angle• Adjust the clearance angle (→ Fig. 22‑1) of the knife holder.

2
1

To this end:
• Loosen the two lateral screws (→ Fig. 22‑2) (Hexagon key, No.
3).
• Use the adjusting lever (→ Fig. 17‑1) to select the desired
clearance angle.
• Secure the selected clearance angle by tightening the twoscrews (→ Fig. 22‑2).

Fig. 22Note
The Leica VT1000 S does not require the readjustment of the clearance angle every time you change
the blade. Make an adjustment only if required by an application for technical reasons (e.g. different
type of tissue).

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Version 2.6, Revision O

Operation

5• To insert the blade, loosen the clamping screw (→ Fig. 23‑1)
located on the knife holder.
• Clean the blade.

1Fig. 23• Insert the blade into the knife holder (→ Fig. 24‑1).
• Secure the blade with clamping screw (→ Fig. 24‑2).

12

Fig. 24

Warning
The blade must fit tightly against the entire length of the inner limit stop of the knife holder.
The blade must be clamped parallel to the front edge of both knife holder clamping jaws.
• Fix the knife holder (→ Fig. 25‑1) with the knife holder clamping
screw (→ Fig. 25‑2).
• Use the REV/FORW rocker button to place the blade edge rightbehind the rear edge (from user's view) of the specimen.
• Pull the UP/DOWN rocker button into the UP direction and keep itin the UP position until the specimen surface is shortly below thelevel of the blade edge (see arrow (→ Fig. 26‑1)).

2

1

Fig. 25

Leica VT1000 S

29

5 Operation

1

Fig. 26

3
2

1• Select sectioning speed and sectioning frequency with the rotaryknobs SPEED and FREQ.
• Use the +/- button to select a sectioning thickness for trimming.
• Select a sectioning range appropriate to the size of the specimen
with the SECTIONING WINDOW button.
• Switch the SINGLE/CONT button to CONT.
Push the START/STOP button.
The instrument will now trim the specimen at the selected
trimming thickness until you push the START/STOP button oncemore.
• Once you have reached the desired specimen plane forsectioning, use the +/- button to select the desired thickness for
sectioning.
• For sectioning proceed as follows:• Select the desired section thickness via the +/- button.
• Switch the SINGLE/CONT button to SINGLE.
• Push the START/STOP button.
The instrument will now produce a section (→ Fig. 27‑1). Whenthe section is finished, the knife will automatically stop at the rearend position behind the specimen (from the user's view).
• Pick up the section as shown on the left using a brush(→ Fig. 27‑2) to mount it on a glass slide (→ Fig. 27‑3).

Fig. 27

5.6

Routine daily maintenance and switching off the instrument - Leica VT1000 S
After all daily procedures have been finished, perform the following:• Switch off the main switch at the back of the instrument.
• Place the magnifier cover on the magnifier.
• Remove the knife holder.
• Take the blade out of the knife holder and dispose it properly and safely.
• Remove the specimen disc and lay it flat on the stage.
• Remove the specimen using a single-edge blade. Then, remove remains of cyanoacrylate adhesivefrom the specimen disc.
• Remove and empty out the buffer tray. Dispose of the contents of the buffer tray properly.
• Drain the cooling bath.
To do so, release the tube from its holder at the rear of the instrument and dispose of the contents of
the ice bath into a suitable vessel. Then wipe off with a dry cloth.
CautionThe contents of the ice bath can become contaminated if buffer solution is spilled over it.

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Malfunctions: Meaning and Troubleshooting
6.

6

Malfunctions: Meaning and Troubleshooting
Warning
When working with directional specimen discs, move the buffer tray to its lowest position directly
after switching on the instrument!
Error messages/symptoms
Sources of error
Collision of knife and specimen Clearance angle adjustment:
disc.
• If a clearance angle wider than
5° is selected, specimen discand knife edge can potentiallycollide with each other.
• When working with directional
specimen holders, knifeedge and specimen holder
can collide at any selected
clearance angle.
Audible warning signal.
Operating error due to locking
function of the REV/FORW button:
Return stroke is not completed.
• With the REV/FORW button
locked the instrument isswitched off via the powerswitch at the rear of the
instrument and is switched on
again without releasing the
REV/ FORW button to its center
position• With the REV/FORW button
locked, the instrument was
switched off via the emergency
stop and after that, theemergency stop was released
again without releasing the
REV/FORW button to its center
position.

Leica VT1000 S

Troubleshooting• Lower the specimen discsufficiently to prevent collision.

• Lower the specimen discsufficiently to prevent collision.

• Unlock the REV/FORW button
by pulling it back to the center
position.
• To reactivate the return stroke
movement, lock the REV/FORW
button again (to REV position).

• Unlock the REV/FORW-button
by pulling it back to the center
position.
• To reactivate the return stroke
movement, lock the REV/
FORW-button again (to REV
position).

31

6 Malfunctions: Meaning and Troubleshooting
Error messages/symptoms
Audible warning signal.
Downward stroke is notcompleted.

The feed motor stops.
Any processing step
(sectioning stroke etc.) isinterrupted immediately.

Sources of error
Troubleshooting
Operating error due to locking• Release the UP/DOWN button
function of the UP/DOWN button:
to its center position.
• To reactivate the downward• With the UP/DOWN button
motion, activate the UP/DOWN
locked in the DOWN position
button again (DOWN).
the instrument was switched
off via the power switch at therear of the instrument switchedon again without releasing the
UP/DOWN button to its center
position.
• With the UP/DOWN button• Release the UP/DOWN button
locked the instrument
to its center position.
was switched off via the• To reactivate the downward
EMERGENCY STOP (foot switch
motion, activate the UP/DOWN
or Emergency stop button) andbutton again (DOWN).
after that the EMERGENCY
STOP was released without
unlocking the UP/DOWN
button.
The EMERGENCY STOP function • Release the EMERGENCY STOP
has been activated.
button.
• Select an operating mode andcontinue working.

Any UP/DOWN motion of
the buffer tray is interrupted
immediately.
Any locked buttons areindicated by an audible
warning signal.
When pressing any key, theinstrument gives an audible
warning signal.
In case the EMERGENCY STOP
function has been activated,
the instrument will remain
inoperational when pressing
the foot switch.
The indication SP is displayed.

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Version 2.6, Revision O

Malfunctions: Meaning and Troubleshooting
Error messages/symptoms
Audible warning signal.

6

Sources of error• Button(s) jammed or defective.
• Locking function /REV or REV/FORW button defective.
• Error on the UP/DOWN button;
DOWN locking function.

Troubleshooting• Push the button several times
to unlock;have defective button replacedby the Technical Service.

• Feed mechanism defective.

• Switch off the instrument; callthe Technical Service.

Error code E0.300 is displayed.

• Important electronic
component defective.

• Switch off the instrument; callthe Technical Service.

Error code E0.400 is displayed.

• Feed motor defective.

• Switch off the instrument; callthe Technical Service.

Error code E.05xx is displayed.

• Light barrier error (forwardfeed)• Switch off the instrument; callthe Technical Service.

Audible warning signal.

• Light barrier error (section
thickness feed)• Switch off the instrument; callthe Technical Service.

• Software detected severe
hardware fault.

• Switch off the instrument; callthe Technical Service.

• STM32 Watch dog reset.

• Instrument can be used as
normal after restart.
• In case of further problems,
call the Technical Service.

Error code E0.1xx is displayed.

xx - there are several error
codes,
00 - there is only one error
code.
Error code E0.200 is displayed.

Error code E0.600 is displayed.

Audible warning signal.
Error code E0.700 is displayed
for approx. 2 secs.

Audible warning signal.
Error code E0.9xx is displayed.

Audible warning signal.
Optical signal via red LED.

Leica VT1000 S• The upper limit of the specimen • Leave the upper limit position
feed has been reached.
(Switch the UP/DOWN button
in DOWN direction).
• Mount a new specimen onto
the specimen holder and startagain.
• The lower limit of the specimen • After unlocking the DOWN
level has been reached (height
position the buffer tray isadjustment of specimen via
automatically raised until thebuffer tray).
audible and optical signals turn
off.

33

6 Malfunctions: Meaning and Troubleshooting
Error messages/symptoms
Audible warning signal.

Sources of error• User has tried to select a
specimen thickness via the+/- button that is below theminimum value (0 μm) or above
the maximum value (999 μm).

Audible warning signal.
(When operating the
instrument for the first time orafter the E-EPROM has been
exchanged.)A clattering sound can beheard.

Troubleshooting• Release the +/- button.

• The warning signal willcease automatically after the
initialization phase.

• The visible clamping screws
have become loose duringsectioning.

• Retighten the loose clamping
screws.

Note
These symptoms may occur from time to time and are unavoidable, as the clamping screws whichhave to be operated by the user cannot be sealed.
Warning
If the clattering sound does not cease once the clamping screws have been retightened, do nothesitate to call the Technical Service immediately.
Do not use the instrument when in this condition.

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Version 2.6, Revision O

Cleaning and Maintenance
7.

Cleaning and Maintenance

7.1

Cleaning the instrument

7

Warning
Always remove the knife / blade before detaching the knife holder from the instrument. Always put
the knife (blade) back into the knife case or blade dispenser when not in use!
When using cleaners, comply with the safety instructions from the manufacturer and the labor-safety
regulations at your laboratory.
When cleaning the outer surfaces, do not use xylene or solvents containing acetone or xylene. The
finished surfaces are not resistant to xylene or acetone!
Ensure that liquids do not enter the interior of the instrument during cleaning.
Before each cleaning, carry out the following preparatory steps:• Switch off the instrument and disconnect the power plug.
• Remove the blade from the knife holder and insert it in the receptacle at the bottom of the blade
dispenser.
• Remove the knife holder for cleaning.
• Remove the specimen plate from the buffer tray and lay it flat on the stage. Carefully remove thespecimen with a single-edge blade.
• Remove section waste using tweezers or a brush.
• Remove the buffer tray, empty it and rinse it separately with water (→ p. 30 – 5.6 Routine daily
maintenance and switching off the instrument - Leica VT1000 S).
Instrument and outside surfaces
If necessary, the varnished outside surfaces of the control panels can be cleaned with a mildcommercial household cleaner or soap water and then be wiped with a cloth.
The instrument must be completely dry before it can be used again.
Cleaning the knife
Warning
When cleaning the knife/blade, always wipe from the knife or blade back towards the cutting edge,
NEVER wipe in the opposite direction - risk of injury!
Clean using an alcohol-based solution.

Leica VT1000 S

35

7 Cleaning and Maintenance
7.2

Changing the fuse
Warning
Before changing a fuse, always switch off the instrument first and remove the instrument cable
completely. The instrument must have cooled down and the paraffin tank must be empty.
When changing a fuse, do NOT use any fuses other than the spare fuses supplied with the
instrument.
If the instrument fails completely, first check the power supply at the power socket.
Then check the fuses at the rear side of the instrument.
To do so, proceed as follows:• Using a screwdriver (→ Fig. 28‑1), carefully push out the fuse insert (→ Fig. 28‑2).
• Remove the fuse insert - it contains two fuses (→ Fig. 28‑3).
• Check that the thin wire (→ Fig. 28‑4) in the glass capillary of a fuse is intact. If not, replace the fuse(the standard scope of delivery includes two replacement fuses).
Warning
Before plugging the power cable back in and switching on the instrument, you must have identified
and corrected the cause of the burned-out fuse.
• Insert the fuse insert with the two fuses and start up the instrument again.
3

1

2

4

Fig. 28

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Version 2.6, Revision O

Ordering Information: Spare Parts, Accessory, Consumables
8.

Ordering Information: Spare Parts, Accessory, Consumables

8.1

Ordering information
Designation

Order No.

Knife holder S
Buffer tray S
Buffer tray S, double-walled
Specimen disc S, Ø 50 mm, non-directional
Magnetic specimen holder, directional
Foot switch with protective housing
Magnifier, complete
Module LED Hi-Power spots, 2-arm
Module Hi-Power Spot, LED 1000
Sapphire knife
Cyanoacrylate glue
Julabo FL300, recirculating cooler/chiller
100 V/50/60 Hz
115 V/50 Hz
230 V/50-60 Hz
230 V/60 Hz
Antifrogen N

8.2

814 0462 30131
14 0462 30132
14 0463 46423
14 0463 27404
14 0462 32060
14 0463 27415
14 0462 31191
14 6000 04826
14 6000 04825
14 0216 39372
14 0371 27414
14 0481 48439
14 0481 48437
14 0481 48436
14 0481 48438
14 0481 45443

Foot switch
Foot switch
The foot switch is an optional accessory which
can be used instead of the START/STOP button.
Order No.: 			

14 0463 27415

Fig. 29

Leica VT1000 S

37

8 Ordering Information: Spare Parts, Accessory, Consumables
8.3

Buffer tray

8.3.1 Double-walled buffer tray S
Note
When using the double-walled buffer tray, the flow cooler must be installed according to theassembly instructions prior to working with specimens.
A clamp for holding the gassing hose for the
buffer in the proper position can be added to the
double-walled buffer tray.
First connect the hoses ((→ Fig. 31‑1), includedin the standard delivery of the double-walledbuffer tray) to the rear of the Julabo Recirculating
Cooler/Chiller FL300, then connect the other end
to the empty buffer tray. Access is easier if you
make the left connection first. To do so, pull backthe lock coupling, attach the hose, and release
the coupling until you hear it click into position.

Fig. 30• Hose set for connecting a recirculating cooler/chiller included.
Order No.: 			

14 0463 46423

1
Fig. 3138

Version 2.6, Revision O

Ordering Information: Spare Parts, Accessory, Consumables
8.4

8Magnifier, LED illumination
Magnifier• To be inserted into the fixture.
Order No.: 			

14 0462 31191

Module LED Hi-Power spots, 2-arm• To be mounted onto the magnifier after themagnifier has been mounted into the fixture.
Then, connect the module LED Hi-Power spots,
2-arm, to the Module Hi-Power spot, LED 1000.
Order No.: 			

14 6000 04826

Fig. 32

Module Hi-Power spot, LED 1000
• Serves as a light source for the module LED HiPower spots, 2-arm.
Order No.: 			

14 6000 04825

Fig. 33

Leica VT1000 S

39

8 Ordering Information: Spare Parts, Accessory, Consumables
8.5

Julabo recirculating cooler/chiller FL300
Recirculating cooler/chiller for connection to the
double-walled buffer tray in the Leica VT1000 S
and Leica VT1200/Leica VT1200 S.
Selectable temperature range: –20 °C to +40 °C.
Recommended cooling medium:
Antifrogen N			
14 0481 45443
Mixture with water (50 %/50 %)Application example:
If (at an ambient temperature of 20 - 22 °C) a
temperature of 4 °C is to be reached in the buffer
trough, the setting value of 0.5 - 2 °C must be
selected.

Fig. 34Note
For additional information, refer to the Instructions for Use provided with this instrument.

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Version 2.6, Revision O

Warranty and Service
9.

9Warranty and Service
Warranty
Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been
subjected to a comprehensive quality control procedure based on the Leica in-house testing standards,
and that the product is faultless and complies with all technical specifications and/or agreed
characteristics warranted.
The scope of the warranty is based on the content of the concluded agreement. The warranty termsof your Leica sales organization or the organization from which you have purchased the contractual
product shall apply exclusively.
Service information
If you are in need of technical customer support or spare parts, please contact your Leica
representative or the Leica dealer where you purchased the instrument.
Please provide the following information:• Model name and serial number of the instrument.
• Location of the instrument and name of the person to contact.
• Reason for the service call.
• Delivery date
Decommissioning and disposal
The instrument or parts of the instrument must be disposed of according to existing applicable, local
regulations.

Leica VT1000 S

41

10 Decontamination Confirmation
10.

Decontamination Confirmation
Every product that is returned to Leica Biosystems or that requires on‑site maintenance must be
properly cleaned and decontaminated. You can find the dedicated template of the decontamination
confirmation on our website www.LeicaBiosystems.com within the product menu. This template has tobe used for gathering all required data.
When returning a product, a copy of the filled and signed confirmation has to be enclosed or passed on
to the service technician. The responsibility for products that are sent back without this confirmation
or with an incomplete confirmation lies with the sender. Returned goods that are considered to be apotential source of danger by the company will be sent back at the expense and risk of the sender.

42

Version 2.6, Revision O

Version 2.6, Revision O - 10.2023

www.LeicaBiosystems.com

Leica Biosystems Nussloch GmbH
Heidelberger Strasse 17 - 1969226 Nussloch
Germany
Tel.: +49 - (0) 6224 - 143 0
Fax: +49 - (0) 6224 - 143 268
Web: www.LeicaBiosystems.com