Aperio GT 450
User’s Guide

MAN-0391, Revision E | March 2022

Aperio GT 450 User’s Guide
This manual applies to Aperio GT 450 Controller, Aperio GT 450 Console, and Aperio GT 450 SAM versions 1.1 and later

Copyright NoticeÌ Copyright © 2019-2022 Leica Biosystems Imaging, Inc. All Rights Reserved. LEICA and the Leica logo are registered trademarks of Leica
Microsystems IR GmbH. Aperio, GT, and GT 450 are trademarks of Leica Biosystems Imaging, Inc. in the USA and optionally in other countries.
Other logos, product and/or company names might be trademarks of their respective owners.

Ì This product is protected by registered patents. For a list of patents, contact Leica Biosystems.

Customer ResourcesÌ For the latest information on Leica Biosystems Aperio products and services, please visit www.LeicaBiosystems.com/Aperio.

Contact Information – Leica Biosystems Imaging, Inc.
Headquarters
Leica Biosystems Imaging, Inc.
1360 Park Center Drive
Vista, CA 92081
USA
Tel: +1 (866) 478-4111 (toll free)Direct International Tel: +1 (760) 539-1100

Customer Support

General Information

Contact your local support representativewith any query and service request.

US/Canada Tel: +1 (866) 478-4111 (toll free)Direct International Tel: +1 (760) 539-1100

https://www.leicabiosystems.com/servicesupport/technical-support/

Email: ePathology@LeicaBiosystems.com

For research use only. Not for use in diagnostic procedures.

23GT450, 23GT450SAM

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Aperio GT 450 User’s Guide, Revision E © Leica Biosystems Imaging, Inc. 2022

Contents
Notices.................................................................................................................................... 7
Revision Record................................................................................................................................7
Intended Use....................................................................................................................................7
Cautions and Notes...........................................................................................................................7
Symbols..........................................................................................................................................8

Customer Service Contacts................................................................................................ 10
1 Introduction..................................................................................................................... 12

Resources......................................................................................................................................13
Warnings......................................................................................................................................13
Electromagnetic Warnings.............................................................................................................13
Instrument Warnings....................................................................................................................14
Component and Part Replacement Warnings......................................................................................14
Compliance Specifications.................................................................................................................14
Installation ....................................................................................................................................15
Disposal of the Aperio GT 450............................................................................................................15

2 Aperio GT 450 Overview................................................................................................. 20
Scanner Overview...........................................................................................................................20
Turn the Scanner On and Off...........................................................................................................21
Touchscreen Interface Overview.........................................................................................................21
Home Screen..............................................................................................................................22
Help Videos and Guides....................................................................................................................24
Key Features..................................................................................................................................24
Continuous Load Workflow............................................................................................................24
Scan Magnification......................................................................................................................24
Automatic Image Quality Check.......................................................................................................24
Image Types Supported.................................................................................................................24
Slide Types Supported..................................................................................................................25
Coverslips..................................................................................................................................25
Slide Racks Supported..................................................................................................................25
Slide Capacity.............................................................................................................................25

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Contents

Barcodes Supported.....................................................................................................................25
Optional Aperio Viewing Station.........................................................................................................25
Aperio Digital Slide Viewers..............................................................................................................25

3 Slide Preparation............................................................................................................ 26
Tissue Preparation...........................................................................................................................26
Staining........................................................................................................................................26
Slide Preparation.............................................................................................................................26
Tissue Placement.....................................................................................................................27
Fixing Slide Preparation Errors.....................................................................................................27
Coverslips......................................................................................................................................27
Labels...........................................................................................................................................28
Barcodes.......................................................................................................................................28

4 Scan Slides...................................................................................................................... 29
Scanning Concepts..........................................................................................................................29
Scan Workflow............................................................................................................................29
Carousel Rotation........................................................................................................................30
Log In and Log Out of the Scanner.......................................................................................................30
Load Slides in the Rack.....................................................................................................................31
Load Racks in the Carousel................................................................................................................32
Priority Scanning.............................................................................................................................34
Rotate a Rack to the Rack Loading Area................................................................................................35
Unload Racks from the Carousel.........................................................................................................36
Check Scan Status...........................................................................................................................37
Check Rack Status.......................................................................................................................37
Rack Error on Current Scan.........................................................................................................38
View Slide Status for a Rack..........................................................................................................38
View Macro Images of Scanned Slides.............................................................................................39
View Rack Scan Order...................................................................................................................39
Rack Warnings............................................................................................................................40
Scan Entire Slide for Whole Rack........................................................................................................41
Image Quality Control for Histotechnicians and Pathologists......................................................................42
Scan Statistics................................................................................................................................43

5 Maintenance.................................................................................................................... 44
Maintenance Schedule.....................................................................................................................44
Serial Number and Firmware Version...................................................................................................45
Shut Down the Scanner....................................................................................................................45
Open the Scanner Cover and Access the Interior Components....................................................................46
Recommended Daily Maintenance......................................................................................................47

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Restart the Scanner......................................................................................................................47
Six Month Maintenance....................................................................................................................48
Clean the Objective and Koehler......................................................................................................48
Materials Needed....................................................................................................................48
Clean the Stage Slide Tray.............................................................................................................51
Clean the Carousel.......................................................................................................................52
Clean the Fan Filter......................................................................................................................53
Clean the Slide Racks...................................................................................................................55
Clean the Scanner Cover...............................................................................................................55
Clean the Touchscreen..................................................................................................................56
Restart the Scanner After Interior Maintenance......................................................................................56
Transporting or Moving the Aperio GT 450............................................................................................57
Long Term Storage...........................................................................................................................57

6 Troubleshooting.............................................................................................................. 58
Personal Protective Equipment...........................................................................................................58
Red Blinking Lights on Carousel..........................................................................................................58
How to Use the Troubleshooting Steps.................................................................................................58
Perform a Safe Restart After an Error...................................................................................................59
Error Codes and Solutions.................................................................................................................61
1000: Internal error......................................................................................................................61
1001: Scanner cannot initialize.......................................................................................................63
1002: Carousel cannot rotate..........................................................................................................65
1003: Carousel cannot rotate. Carousel pinch point obstructed...............................................................67
1007: Internal storage full. Cannot send images to DICOM converter........................................................67
2000: Slide handling error at slide stage, rack, or pusher.......................................................................67
2001: Slide handling error at rack gripper, lift, or carousel.....................................................................70
9000: Scanner cover is open...........................................................................................................72
Rack Warnings and Solutions.............................................................................................................72
1005: Cannot process rack.............................................................................................................72
1006: Cannot process one or more slides in rack.................................................................................73
Slide Errors and Solutions..............................................................................................................74
Tilted Slide(s)..........................................................................................................................74
No Barcode.............................................................................................................................75
No Tissue...............................................................................................................................75
No Macro Focus.......................................................................................................................75
Image Quality..........................................................................................................................76
Image Transfer Error – Pending Retry............................................................................................77
Aborted.................................................................................................................................77
Aborted Message Appears on All Slides........................................................................................78

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Symptoms and Solutions...................................................................................................................79
Scanner does not power on............................................................................................................79
Touchscreen does not respond to touch.............................................................................................79
Touchscreen is black.....................................................................................................................79
Slides are broken inside the scanner................................................................................................80
Internet connection lost.................................................................................................................80

Index...................................................................................................................................... 81

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Notices
Revision Record
Rev.

IssuedSections Affected

DetailE

March 2022

Front matter, Compliance
Specifications, Chapter 4,“Scan Slides,” Chapter 6,“Troubleshooting”

Added revision history, cautions and notes, updated compliancespecifications.
Chapter 4: Updated scan workflow diagram to include manual image quality
check. Added new section “Image Quality Control for Histotechnicians andPathologists.” Chapter 6: Added troubleshooting section for lost Internet
connection.

D

April 2020

All

Changed references to two monitors to “monitor(s)” to accommodatechange in product configuration.

C

November 2019 All

Updated graphics for patch 1.0.1

B

October 2019

All

Changes for patch 1.0.1. Updated graphics, added new section “Scan Entire
Slide for Whole Rack.”A

June 2019All

New document.

Intended Use
For research use only. Not for use in diagnostic procedures.

Cautions and Notes`

Serious Incidents Reporting - Any serious incident that has occurred in relation to the Aperio GT 450 shall bereported to the manufacturer and the competent authority of the member state in which the user is established.

`

Specifications and Performance - For device specifications and performance characteristics, refer to the
document Aperio GT 450 Specifications.

`

Installation - Aperio GT 450 must be installed by a trained Leica Biosystems Technical Services representative.

`

Repair - Repairs may be done only by a trained Leica Biosystems Technical Services representative. After repairs
are done, ask the Leica Biosystems technician to perform operation checks to determine the product is in good
operating condition.

`

Accessories - For information on using Aperio GT 450 with third-party accessories such as a Laboratory
Information System (LIS) not provided by Leica Biosystems, contact your Leica Biosystems Technical Services
representative.

`

Quality Control - For information on image quality checks, see “Chapter 4: Scan Slides” on page 29.

`

Maintenance and Troubleshooting - For information on maintenance and troubleshooting problems, see“Chapter 5: Maintenance” on page 44 and “Chapter 6: Troubleshooting” on page 58.

`

Cybersecurity - Be aware that workstations are susceptible to malware, viruses, data corruption, and privacy
breaches. Work with your IT administrators to protect workstations by following your institution’s password andsecurity policies. For Aperio recommendations on protecting your workstations and servers, see the document
Aperio GT 450 IT Manager and Lab Administrator Guide.

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To protect workstations from malware intrusion, use caution when inserting USB drives and other removabledevices. Consider disabling USB ports that are not in use. If you plug in a USB drive or other removable device, you
should scan the devices with an anti-malware utility. For recommendations on protecting your workstations andservers, see the document Aperio GT 450 IT Manager and Lab Administrator Guide.
If a suspected Aperio GT 450 cybersecurity vulnerability or incident is detected, contact Leica Biosystems Technical
Services for assistance.
`

Training - This manual is not a substitute for the detailed operator training provided by Leica Biosystems or forother advanced instruction.

`

Safety - Safety protection may be impaired if this device is used in a manner not specified by the manufacturer.

Symbols
The following symbols appear on your product label or in this user’s guide:

Symbol8Standard/
Regulation

Description

ISO 15223-1 - 5.1.1

Manufacturer

ISO 15223-1 - 5.1.3

Date of manufacture

ISO 15223-1 - 5.1.7

Serial number

ISO 15223-1 – 5.1.6

Catalog number

ISO 15223-1 - 5.4.4

CautionSO 7010 – W001

General warning

IEC 61010-1

TÜV Product Services have certified that the listed products comply with both U.S and Canadiansafety requirements.

IEC 60417 - 5031

This device is suitable for direct current only.

Aperio GT 450 User’s Guide, Revision E © Leica Biosystems Imaging, Inc. 2022

SymbolSymbolStandard/
Regulation

Description

IEC 60417 - 5007

On. To indicate connection to the mains, at least for mains switches or their positions, and thosecases where safety is involved.

IEC 60417 - 5008

Off. To indicate disconnection from the mains, at least for main switches, and all those cases
where safety is involved.

2012/19/EU

Device is regulated under 2012/19/EU (WEEE Directive) for Electrical and Electronic Equipment
Waste and must be discarded under special conditions.

People’s Republicof China Electronic
Industry Standard SJ/
T11364

Device contains certain toxic or hazardous elements and can be used safely during its
environmental protection use period. The number in the middle of the logo indicates theenvironmental protection use period (in years) for the product.

Standard/
Regulation

Description

IEC 60825-1

Device is a Class 1 Laser Product that is in compliance with international
standards and US requirements.

CA Proposition 65

This product can expose you to chemicals known to the State of California
to cause Cancer and Reproductive Harm. For more information go tohttps://www.P65Warnings.ca.gov.

N/A

Device is made in the USA of US and foreign components.

Aperio GT 450 User’s Guide, Revision E © Leica Biosystems Imaging, Inc. 2022

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Customer Service Contacts
Please contact the office for your country for technical assistance.
Australia:
96 Ricketts Road
Mount Waverly, VIC 3149
AUSTRALIA
Tel: 1800 625 286 (toll free)
Between 8:30 AM-5 PM, Monday-Friday, AEST
Email: lbs-anz-service@leicabiosystems.com

Deutschland:
Leica Biosystems Nussloch GmbHTechnical Assistance Center
Heidelberger Strasse 17
Nussloch 69226
GERMANY
Tel: 0080052700527 (toll free)
In-country Tel: +49 6441 29 4555
Email: support.de@leicabiosystems.com

Austria:
Leica Biosystems Nussloch GmbHTechnical Assistance Center
Heidelberger Strasse 17
Nussloch 69226
GERMANY
Tel: 0080052700527 (toll free)
In-country Tel: +43 1 486 80 50 50
Email: support.at@leicabiosystems.com

Eire:
Tel: 0080052700527 (toll free)
In-country Tel: +44 1908 577 650
Email: support.ie@leicabiosystems.com
España:Tel: 0080052700527 (toll free)
In-country Tel: +34 902 119 094
Email: support.spain@leicabiosystems.com

België/Belgique:Tel: 0080052700527 (toll free)
In-country Tel: +32 2 790 98 50
Email: support.be@leicabiosystems.com

France:Tel: 0080052700527 (toll free)
In-country Tel: +33 811 000 664
Email: support.fr@leicabiosystems.com

Canada:Tel: +1 844 534 2262 (toll free)Direct International Tel: +1 760 539 1150
Email: TechServices@leicabiosystems.com
China:
17F, SML Center No. 610 Xu Jia Hui Road, Huangpu District
Shanghai, PRC PC:200025
CHINA
Tel: +86 4008208932
Fax: +86 21 6384 1389
Email: service.cn@leica-microsystems.com
Remote Care email: tac.cn@leica-microsystems.com
Danmark:
Tel: 0080052700527 (toll free)
In-country Tel: +45 44 54 01 01
Email: support.dk@leicabiosystems.com

10

Italia:Tel: 0080052700527 (toll free)
In-country Tel: +39 0257 486 509
Email: support.italy@leicabiosystems.com
Japan:
1-29-9 Takadanobaba, Shinjuku-ku
Tokyo 169-0075
JAPAN
Nederland:
Tel: 0080052700527 (toll free)
In-country Tel: +31 70 413 21 00
Email: support.nl@leicabiosystems.com

Aperio GT 450 User’s Guide, Revision E © Leica Biosystems Imaging, Inc. 2022

New Zealand:
96 Ricketts Road
Mount Waverly, VIC 3149
AUSTRALIA
Tel: 0800 400 589 (toll free)
Between 8:30 AM-5 PM, Monday-Friday, AEST
Email: lbs-anz-service@leicabiosystems.com
Portugal:
Tel: 0080052700527 (toll free)
In-country Tel: +35 1 21 388 9112
Email: support.pt@leicabiosystems.com
The Russian Federation
BioLine LLC
Pinsky lane 3 letter A
Saint Petersburg 197101
THE RUSSIAN FEDERATION
Tel: 8-800-555-49-40 (toll free)
In-country Tel: +7 812 320 49 49
Email: main@bioline.ru
Sweden:Tel: 0080052700527 (toll free)
In-country Tel: +46 8 625 45 45
Email: support.se@leicabiosystems.com
Switzerland:
Tel: 0080052700527 (toll free)
In-country Tel: +41 71 726 3434
Email: support.ch@leicabiosystems.com
United Kingdom:
Tel: 0080052700527 (toll free)
In-country Tel: +44 1908 577 650
Email: support.uk@leicabiosystems.com
USA:
Tel: +1 844 534 2262 (toll free)Direct International Tel: +1 760 539 1150
Email: TechServices@leicabiosystems.com

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1

Introduction

The Aperio GT 450 is a high performance, brightfield whole slide scanner that includes continuous loading with 450 slidecapacity across 15 racks, priority rack scanning, automated image quality check and a scan speed of ~32 seconds at 40x
scanning magnification for a 15 mm x 15 mm area.
This system is intended for use by trained histotechnicians, IT professionals, and pathologists.
Pathology Lab

IT Room

IT Manager’s Office

Pathologist’s Office

SAM Application Software

Aperio Viewing Station

SAM server

Aperio GT 450s

DSR server

Use of the Aperio GT 450 scanner requires the following components.

Component

Description

Scanner Administration Manager(SAM) Server

The SAM server connects to multiple Aperio GT 450 scanners and runs the
SAM Application Software.

SAM Application Software

The Scanner Administration Manager (SAM) client application software
enables IT implementation, PIN configuration, and service access of multiple
scanners from a single desktop client location for IT professionals.

Aperio Viewing Station

The viewing station includes a calibrated monitor(s) and a workstation with
Aperio ImageScope version 12.4 or higher.

See the Aperio GT 450 Specifications for more information on these components.
Ensure you follow appropriate good laboratory practices and the policies and procedures required by your institution forslide preparation, processing, storage, and disposal. Use this equipment only for this purpose and in the manner describedin this guide.

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Chapter 1: Introduction

Resources
Resource

Description

Aperio GT 450 User’s Guide

Provides reference information and instructions for scanning, troubleshooting,and maintenance.

Aperio GT 450 Quick Reference

Contains quick instructions for scanning, basic troubleshooting, andmaintenance. We recommend keeping this printed guide with your scanner.
An electronic version of the quick reference guide is available from the Helparea on the scanner’s touchscreen interface.

Aperio GT 450 IT Manager and Lab
Administrator Guide

Contains information and instructions regarding IT administration of the scanner,
including network administration and configuration.

Aperio GT 450 Specifications

Provides detailed specifications for the scanner.

Instructional videos

Provides video instruction on performing various tasks. You can view the videos
from the Help area on the scanner’s touchscreen interface.

Customer Support

If you have an issue with the scanner you cannot resolve, contact Leica
Biosystems Technical Services. See page 2 for contact information.

Training

Leica Biosystems Imaging offers classroom and virtual training courses. Contact
Leica Biosystems Imaging Customer Support for information about training
options.

Warnings
The following symbols are used throughout the documentation to alert you to potential danger in operating the scanner.

SymbolDescription
The exclamation point within an equilateral triangle is intended to alert you to the presence ofimportant operating and maintenance (servicing) instructions.
Le point d’exclamation dans un triangle équilatéral vise à avertir l’utilisateur qu’il s’agit d’instructions
d’utilisation et d’entretien importantes.
Laser Compliance. This symbol indicates that the product is a Class 1 Laser Product and is incompliance with international standards and US requirements.

Electromagnetic Warnings
If the scanner is used in any manner not specified in this documentation, the protection provided by the equipment may be
impaired.
This device complies with the emissions and immunity requirements as specified in the EN/1EC 61326 series of Product
Family Standards for a “basic electromagnetic environment.” Such equipment is supplied directly at low voltage from publicmains network. This equipment is not intended for residential use.

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Chapter 1: Introduction

This device generates, uses, and can radiate unintentional radio-frequency (RF) energy. If this device is not installed andoperated correctly, this RF energy can cause interference with other equipment. It is the responsibility of the end user tobe sure that a compatible electromagnetic environment for the device can be maintained so that the device operates as
intended.
This equipment is designed for use in a PROFESSIONAL HEALTHCARE FACILITY ENVIRONMENT. It is likely to perform
incorrectly if used in a HOME HEALTHCARE ENVIRONMENT. If it is suspected that performance is affected byelectromagnetic interference, correct operation may be restored by increasing the distance between the equipment and thesource of the interference.
In addition, other equipment can radiate RF energy to which this device is sensitive. If one suspects interference between
this device and other equipment. Leica Biosystems recommends the following actions to correct the interference:`

Evaluate the electromagnetic environment before installation and operation of this device.

`

Do not operate this device close to sources of strong electromagnetic radiation (for example: unshielded intentional
RF sources), as these can interfere with proper operation. Examples of unshielded intentional radiators are
handheld radio transmitters, cordless phones, and cellular phones.

`

Do not place this device near medical electrical equipment that can be susceptible to malfunctions caused by close
proximity to electromagnetic fields.

`

This device has been designed and tested to CISPR 11. Class A emission limits. In a domestic environment, thisdevice can cause radio interference, in which case, you need to take measures to mitigate the interference.

Instrument Warnings
WARNING: TO REDUCE THE RISK OF FIRE OR ELECTRIC SHOCK, DO NOT EXPOSE THE
SCANNER TO RAIN OR MOISTURE.
MISUSE OF ELECTRICAL EQUIPMENT CAN CAUSE ELECTROCUTION, BURNS, FIRES,
AND OTHER HAZARDS.
AVERTISSEMENT : POUR RÉDUIRE LES RISQUES D’INCENDIE OU DE CHOC ÉLECTRIQUE,
NE PAS EXPOSER CET APPAREIL À LA PLUIE OU L’HUMIDITÉ.
UN MAUVAIS USAGE D’UN APPAREIL ÉLECTRIQUE PEUT PROVOQUERL’ÉLECTROCUTION, DES BRÛLURES ET D’AUTRES DANGERS.

Component and Part Replacement Warnings
There are no user-replaceable parts or components in the Aperio GT 450 scanner. Replacement of parts or components
within the Aperio GT 450 scanner must be performed by qualified Leica Biosystems Technical Services personnel.

Compliance Specifications
This device complies with Part 15 of the FCC rules. Operation is subject to the following two conditions: (1) This device may
not cause harmful interference and (2) this device must accept any interference received, including interference that may
cause undesired operation.

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Chapter 1: Introduction

This device has been evaluated against and conforms to the following standards:

FeatureDetails

SafetyIEC 61010-1: 2010
IEC 61010-1:2010/AMD1:2016
IEC 61010-2-081: 2018
UL 61010-1:2012/R:2019-07
UL 61010-2-081:2019
CAN/CSA C22.2 No. 61010-1:2012/A1:2018
CAN/CSA C22.2 No. 61010-2-081:2019
EN IEC 6010-2-081:2020
EN 61010-1:2010/A1.2019
EMC

EN 61326 (Emissions)
VCCI CISPR 32
KN 32
FCC/IC

Installation
WARNING: Two people are required for lifting the scanner.
AVERTISSEMENT: Il faut deux personnes pour soulever l’appareil.

Installation and setup of the scanner should only be performed by a trained Leica Biosystems Imaging Service
representative. After installation, the Leica Biosystems Service representative will check the scanner for proper operation.
When you first receive the Aperio GT 450 and accessories, inspect the pallet and attached boxes for any damage. If the
package has visible damage from shipment, contact Leica Biosystems Customer Service for assistance.

Disposal of the Aperio GT 450
The service life of the Aperio GT 450 is five years. After that time, cybersecurity patches and other technical support may not
be provided.
This device is regulated as Electrical and Electronic Equipment Waste (WEEE) under 2012/19/EU and must be discarded
under special conditions. Contact Leica Biosystems, Inc. for assistance or questions regarding device disposal.

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Chapter 1: Introduction

Scanner Safety Instructions
This section contains important safety information for the Aperio GT 450 scanner.
When using your scanner, always take basic safety precautions, including all of those listed below.
`

Read All Instructions – All safety and operating instructions must be read before operating the product.

`

Retain All Instructions – All safety and operating instructions must be retained for future reference.
Lisez toutes les instructions - Vous devez lire toutes les consignes de sécurité et d’utilisation avant d ‘utiliser
l’appareil.
Conservez toutes les instructions - Vous devez conserver toutes les instructions à des fins de consultation
ultérieure.

`

Heed All Warnings – All warnings on the scanner and those listed in the operating instructions must be adheredto.
Tenez compte des avertissements – Vous devez respecter tous les avertissements à propos du numériseur ainsi
que de ceux indiqués dans les instructions d’utilisation.

`

Follow All Instructions – All operating and scanner usage instructions must be followed.
Suivez toutes les instructions – Vous devez suivre toutes les instructions d’utilisation du numériseur.

1. Heat – The scanner must be situated away from any heat sources such as radiators, heat registers, stoves, orother products that produce heat.
Chaleur – L’appareil doit être placé loin de toutes sources de chaleur telles que les radiateurs, les bouches dechaleur, les cuisinières ou autres appareils produisant de la chaleur.
2. Ventilation – Slots and openings in the scanner are provided for ventilation. They ensure reliable operation of
the product, keeping it from overheating. These openings must not be blocked nor covered during operation. Keep
air openings free of lint, hair, fluff, etc. This product should not be placed into a rack unless proper ventilation isprovided through following the manufacturer’s recommended installation procedures.
Ventilation – L’appareil est doté de fentes et d’ouvertures de ventilation. Elles assurent le fonctionnement
fiable de l’appareil, en l’empêchant de surchauffer. Ces ouvertures ne doivent pas être bloquées, ni couvertes
pendant l’utilisation. Gardez les ouvertures libres de charpie, de cheveux, de peluches, etc. Cet appareil ne doitpas être placé dans un bâti à moins qu’une ventilation adéquate ne soit fournie en suivant le mode d’installation
recommandé par le fabricant.
3. Biosafety Procedures – Please refer to your institution’s biosafety policies and procedures regarding the proper
handling of tissue and other potentially hazardous materials in connection with the use of this device.
Procédure de biosécurité – Veuillez vous référer aux politiques et aux procédures de biosécurité de votreétablissement relativement à la manipulation adéquate de tissus et autres matières potentiellement dangereusesen lien avec l’utilisation de cet appareil.
4. Water and Moisture – Do not use the scanner near water—for example, near a water bath, wash bowl, kitchensink, or laundry tub; in a wet basement; or near an open pool of water or the like. If the scanner becomes wet,
unplug it before touching it.
Eau et humidité – N’utilisez pas l’appareil près de l’eau, par exemple, un bain-marie, une cuvette, un évier, une

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Chapter 1: Introduction

cuve de lessivage, dans un sous-sol humide ou près d’un bassin ouvert. Si l’appareil est mouillé, débranchez-leavant de le toucher.
5. Attachments – Do not use any attachments not recommended by the product manufacturer as they may cause
hazards.
Accessoires – N’utilisez pas d’accessoires non recommandés par le fabricant de l’appareil, car ils peuvent causer
un risque.
6. Power Sources – The scanner must be operated from the type of power source indicated on the marking label
and in the installation instructions. If you are not sure of the type of power supplied to your facility, consult yourlocal power company. Check that the voltage setting matches the electrical supply voltage.
Alimentation – L’appareil ne doit être utilisé qu’avec la source d’alimentation spécifiée sur l’étiquette signalétiqueet les instructions d’installation. En cas de doute sur le type de courant fourni à votre établissement, consultez
votre compagnie d’électricité locale. Vérifiez si le réglage de la tension correspond à la tension d’alimentationélectrique.
7. Grounding and Polarization – The scanner AC/DC adapter is equipped with a polarized AC plug with integral
safety ground pin. Do not defeat the safety ground in any manner.
Mise à la terre et polarisation – L’adaptateur c.a. du numériseur est muni d’une fiche c.a. polarisée à contact
intégré de mise à la terre de sécurité. En aucun cas, ne retirez la mise à la terre de sécurité.
8. Power Cord Protection – Power supply cords must be routed so that they are not likely to be walked on
nor pinched by items placed upon or against them. Pay particular attention to the cords at AC wall plugs andconvenience receptacles and at the point where the cord enters the AC/DC adapter.
Protection du cordon d’alimentation – Les cordons d’alimentation doivent être placés de manière à ce qu’ily ait peu de risque qu’une personne marche dessus ou qu’ils soient coincés par un meuble ou un autre objet.
Veillez particulièrement à protéger le cordon près des prises murales c.a., des prises utilitaires et de sa sortie del’appareil.
9. Lightning – For added protection for this scanner during a lightning storm, or when it is left unattended andunused for long periods of time, unplug it from the AC wall outlet. This will prevent damage to the product due tolightning and power line surges.
Foudre – Pour protéger davantage l’appareil, débranchez le cordon du numériseur de la prise murale c.a. pendant
un orage ou s’il est laissé sans surveillance ou qu’il n’est pas utilisé pendant une longue période. Cette mesureempêchera que la foudre ou qu’une surtension cause des dommages à l’appareil.
10. Power Overloading – Do not overload AC wall outlets, extension cords, or integral convenience outlets as thiscan result in a fire or electric shock hazard.
Surcharge – Ne surchargez pas les prises murales c.a., les rallonges ou les prises utilitaires intégrées car ceci
risquerait de provoquer un incendie ou une électrocution.
11. Operating Environment – Observe these basic safety requirements:
Milieu de fonctionnement – Respectez ces consignes de sécurité de base:`

Do not operate the scanner out of doors. N’utilisez pas l’appareil à l’extérieur.

`

Do not use where aerosol sprays are being used or where oxygen is being administered. Ne l’utilisez pas à
proximité d’aérosols ou d’endroits où de l’oxygène est administré.

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Chapter 1: Introduction`

Do not let the scanner or its cables or accessories come into contact with surfaces which are too hot to touch.
Ne laissez pas l’appareil ou ses câbles et ses accessoires entre en contact avec des surfaces qui sont trop
chaudes au toucher.

`

Do not place anything on top of the scanner. Ne placez rien sur l’appareil.

12. Object and Liquid Entry – Never push objects of any kind into the scanner through openings as they may touch
dangerous voltage points or short-out parts that could result in a fire or electric shock. Never spill liquid of any kindon the scanner.
Pénétration d’objets et de liquides – N’introduisez jamais aucun objets par les orifices du numériseur. Ils pourraient
toucher des points sous tension dangereux et court-circuiter des pièces et provoquer ainsi un incendie ou uneélectrocution. Veillez à ne jamais renverser de liquide dans l’appareil.
13. Accessories – Do not place the scanner on an unstable cart, stand, tripod, bracket, or table. The scanner may
fall, causing serious injury and damage to the product. Any mounting of the scanner needs to follow manufacturer’s
installation instructions. Do not place anything on top of the scanner.
Accessoires – Ne placez pas l’appareil sur un chariot, un socle, un trépied, un support ou une table qui n’est pas
stable. L’appareil pourrait tomber et provoquer une blessure grave ou être endommagé. Si l’appareil est fixé à unsupport, assurez-vous que les instructions d’installation du fabricant sont respectées.
14. Moving – Use care if you must slide the scanner around the workbench. If you need to pick up the scanner to moveit off the workbench, contact Leica Biosystems Technical Services. Removal of the scanner may void the applicableproduct warranty—contact Leica Biosystems Imaging for advice.
Déplacement – L’appareil doit être déplacé avec prudence. Un arrêt brusque, une force excessive ou une surface
inégale peuvent faire tomber l’appareil. Le déplacement d’un appareil peut annuler la garantie – communiquez
avec Leica Biosystems Imaging pour obtenir des conseils.
15. Servicing – Refer all servicing to qualified service personnel.
Entretien – Faites appel à un technicien qualifié pour tout entretien.
16. Damage Requiring Service – Unplug the scanner from the wall AC outlet and refer servicing to qualified service
personnel under the following conditions:
Dommage nécessitant un entretien – Débranchez l’appareil de la prise murale c.a. et faites appel à untechnicien qualifié dans les cas suivants:

18`

When the AC cord or AC/DC adapter is damaged. Le cordon c.a. ou la fiche est endommagé.

`

If liquid has been spilled or objects have fallen into the scanner. Du liquide a été renversé sur l’appareil ou desobjets sont tombés dans l’appareil.

`

If the scanner has been exposed to rain or water. L’appareil a été exposé à la pluie ou à l’humidité.

`

If the scanner does not operate normally (when you are following operating instructions). L’appareil nefonctionne pas normalement (lorsque vous suivez les instructions d’utilisation).

`If the scanner has been dropped or damaged in any way. L’appareil est tombé ou est endommagé de quelquefaçon que ce soit.

`

When the scanner exhibits a distinct change in performance. This indicates a need for service. L’appareil
présente des changements visibles de performances. Cela indique la nécessité d’un entretien.

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17. Replacement Parts – When replacement parts are required, be sure the service technician has used replacement
parts specified by the manufacturer. Unauthorized substitutions may result in fire, electric shock, or other hazards.
The objective in the Aperio GT 450 scanner has been specifically designed for this device. Do not replace it withanother type of objective.
Pièces de rechange – Si des pièces doivent être remplacées, assurez-vous que le technicien utilise bien lespièces de rechange prescrites par le fabricant. L’utilisation des pièces non autorisées peut entraîner un risqued’incendie ou de choc électrique, ou d’autres dangers.
18. Operational Check – Upon completion of any repairs to this scanner, ask the service technician to perform
operation checks to determine that the product is in proper operating condition.
Vérification du fonctionnement – Après toute réparation d’un appareil, demandez au technicien d’effectuer lesvérifications nécessaires pour s’assurer que l’appareil est en bon état de fonctionnement.
19. Cleaning – Apply the recommended cleaning solution to a clean cloth to clean the equipment. Do not apply
cleaning solutions directly to the equipment.
Nettoyage – N’utilisez pas de nettoyants liquides ou aérosols. Utilisez seulement un chiffon humide pour lenettoyage.

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2

Aperio GT 450 Overview

This chapter provides an overview of the Aperio GT 450 Scanner.

Scanner Overview
This section describes the parts of your scanner used in daily operation.
Front

Back

Fan filter
Touchscreeninterface

CarouselStatus lights (6)On/off switch
Rack loading area (front 6)

Power / network connections

The following section describes the main scanner elements:

Element:

Use:

More details:

Touchscreen Interface

View rack statuses, set rack options, and view otherinformation.

See “Touchscreen Interface
Overview” on page 21.

Carousel

The carousel contains 15 rack slots. After you load
racks in the carousel, the carousel rotates the racks
to the scanning area and the rack loading area.

See “Carousel Rotation” on page
30.

Rack loading area

Provides access to the carousel to load and unload
slide racks. This area is also referred to as the “frontsix,” because the rack loading area gives you accessto six rack slots at a time.

See “Load Racks in the Carousel” onpage 32.

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Element:

Use:

Safety light curtain

The infrared safety light curtain detects objects in the See “Carousel Rotation” on pagerack loading area. The carousel only rotates when the 30.
safety light curtain area is clear.

Status lights

The status lights are located below the rack slots
in the rack loading area. They indicate the statusof the front-six racks and the scanner. The colors of
the status lights match the colors of the rack status
Legend on the touchscreen interface.

See “Check Scan Status” on page37.

The fan filter is located on the back of the scanner.
Clean the fan filter at least every six months. You caninsert the fan filter from the left side, the right side,or the top (requires a different fan filter holder).

See “Clean the Fan Filter” on page53.

Fan filter

More details:

See “Red Blinking Lights on
Carousel” on page 58.

Turn the Scanner On and Off
The On/Off switch is located on the right side of the scanner near the back.
`On position = I; Off position = O.

When you are turning the scanner off and on, follow these procedures according to the situation:`“Shut Down the Scanner” on page 45.

`“Restart the Scanner After Interior Maintenance” on page 56.

`“Perform a Safe Restart After an Error” on page 59.

Touchscreen Interface Overview
The touchscreen interface is where you view the scan status and interact with the scanner. Tap an area on the touchscreeninterface to view information or perform an action.

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Home Screen
The Home screen (Carousel view) is where you view rack and slide status. The buttons along the top enable you to accessother areas, such as Help and Maintenance.
Help videos and guides

Log in to the scanner

Maintenance area
Scanner statistics
Home screen(carousel view)

Rack view
Slide view
Rack order
Carousel and rack status

Selected rack
Rack status legend
Rack actions
Slide view, rack order,
rack view appear here

Rack settings

Home screen (carousel view), scanner statistics, help videos and guides appear here

The following section describes the Home screen elements.

Element:

Use:

More details see:

Rack status legend The Legend defines colors and icons that indicate
rack status. Status colors appear on the rack slot
positions on the touchscreen interface, and on thestatus lights in front of the carousel.

“Check Scan Status” on page 37.

Note: the Legend is for information only and is notinteractive.
Scanner statistics

22

Tap Stats to view scan statistics, such as
the number of slides or racks scanned
within a certain time period.

“Scan Statistics” on page 43.

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Chapter 2: Aperio GT 450 Overview

Element:

Use:

More details see:

Maintenance

Tap Maintenance if you need to restart
the scanner, or view the serial number andthe hardware and firmware versions of yourscanner.

“Serial Number and Firmware Version” onpage 45.

Help

Tap Help to view training and maintenance
videos, or access an online version of thequick reference guide.

“Help Videos and Guides” on page 24.

Carousel and rack
statusUse the carousel and rack position on thetouchscreen interface to:“Carousel Rotation” on page 30.

“Shut Down the Scanner” on page 45.
“Restart the Scanner” on page 47.

“Check Scan Status” on page 37.

y View the rack status, indicated by thecolor of the rack position.
y Tap a rack position to select the rack.
y Apply an action to the selected rack,such as Priority or Rotate (describednext).
Rack actions
Note: Rack actions
appear when you
select a rack.
Rack settings
Note: Rack settings
appear when you
select a rack.
Login, Logout

Select a rack and tap Priority to move therack to the top of the scan queue.

“Priority Scanning” on page 34.

Select a rack and tap Rotate to rotate therack to the rack loading area.

“Rotate a Rack to the Rack Loading Area”
on page 35.

Select a rack and tap Settings to selectand apply a rack setting.

“Scan Entire Slide for Whole Rack” onpage 41.

Tap Login to open a keypad and enter yourpass code to access the scanner.

“Log In and Log Out of the Scanner” onpage 30.

Tap Logout to lock access to the scanner
touchscreen interface.
Rack View

Select a rack and tap Rack View to view
the scan status for each slide in the rack.

“View Slide Status for a Rack” on page38.

Slide View

Select a rack and tap Slide View to view
macro images for each slide.

“View Macro Images of Scanned Slides”
on page 39.

Rack Order

Tap Rack Order to view scan order andstatus of the racks.

“View Rack Scan Order” on page 39.

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Help Videos and Guides
You can access training videos and the Aperio GT 450 Quick Reference Guide directly from the touchscreen interface. We
recommend you watch the training videos before operating the scanner for the first time.
1. Tap Help, and then tap a video or guide to view.

Key Features
This section describes some key features of your Aperio GT 450.

Continuous Load Workflow
You can continuously load new racks and unload completed racks without interruption.

Scan Magnification
Custom optics for native 40x scanning magnification.

Automatic Image Quality Check
The Auto-Image QC automatically checks scan quality. If the scan status is green for “complete,” the scan and Image QC
were successful. If there is a problem with the scan quality on a slide, the system alerts you.

Image Types Supported
The Aperio GT 450 creates DICOM compatible images and SVS files.

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Chapter 2: Aperio GT 450 Overview

Slide Types Supported
The scanner supports 1 x 3 inch glass slides (per ISO 8037/1).

Coverslips
Fully-cured coverslips are required. The Aperio GT 450 supports common industry coverslips used for 1 x 3 inch slides.

Slide Racks Supported
Optimized and recommended for use with Leica HistoCore Spectra workstation racks (stainer and coverslipper), which
include the Leica Universal Rack 30-slide capacity (part number 23RACKGT450). Sakura Prisma Stainer and Coverslipper
Rack 20-slide capacity racks also accepted.

Slide Capacity
The maximum slide capacity depends on the racks used:`

20-slide racks – load up to 300 glass slides.

`

30-slide racks – load up to 450 glass slides.

Barcodes Supported
The Aperio GT 450 supports the following barcodes:
y NW7

y Code 39

y QR Codey Code 128

y Data Matrix

y PDF417

y Interleaved 2 of 5

y MicroPDF41

Optional Aperio Viewing Station
We recommend using the Aperio Viewing Station for slide image viewing. The Aperio Viewing Station is optional and is notincluded in the Aperio GT 450 product. For more information, contact your Leica Biosystems representative.
If you do not purchase the viewing station, we recommend using a calibrated monitor for optimal image viewing.
For detailed viewing station and monitor specifications and requirements, see the Aperio GT 450 Specifications.

Aperio Digital Slide Viewers
You can use the following viewers to view digital slides created on the Aperio GT 450 scanner:`

Aperio ImageScope (version 12.4 or higher) – this viewer is installed on your viewing workstation. For thelatest version of Aperio ImageScope, contact your Lab Manager or download the installation file from the Leica
Biosystems website. You must have administrator privileges on your viewing station to install Aperio ImageScope.

`

Aperio WebViewer – this viewer is integrated with Aperio eSlide Manager. It is also available for LIS
integration.

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3

Slide Preparation

When preparing slides for scanning, you must use the procedures required by the vendors of the stains and reagents
you are using.

i

Well prepared slides are crucial to a successful scan. After reading this section, if you have questions about whether
your slide preparation techniques will provide good scan quality, contact Leica Biosystems Technical Services.
Always consult the material safety data sheets for the stains and re-agents you are using, as well as your institution’s
biosafety policies and procedures regarding safely handling biological materials.
It is the responsibility of the lab to verify the quality of the tissue preparation, the physical characteristics of the slide, andthe staining quality.

Tissue Preparation
For optimal scanner performance, slides must be well-prepared.

Staining
Reproducibility of the slide staining is important for consistent and accurate diagnosis.
`

Make sure that the variations of the staining process are controlled and eliminated to the greatest extent possible.

`

Use appropriate morphological studies and controls as specified in the reagent manufacturer’s instructions.

`

Avoid excessive stain residue, as this may affect scanner performance.

Slide Preparation
Many scanning issues are avoided by checking slide quality. Ensure the following:

26`

Slides are very clean and in good condition. Wipe them with a clean cotton cloth (don’t use chemicalcleaners). Make sure the slides have no dirt, no fingerprints, no markings, no writing, no extra mounting media, no
broken slides, no chips, no scratches, etc.

`

Slides are fully cured (not “wet”).

`

All slides have coverslips. Coverslips are required when using the Aperio GT 450 scanner.

`

There is no mounting adhesive around the edges of a slide. This can cause it to stick or catch in the scannerstage area.

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CAUTION: Do not attempt to scan damaged slides, as doing so may damage the scanner.

Tissue Placement
Place the tissue in the middle of the slide, a distance from the edges of the slide, the label, and any other markings. Ensure
the following:`

The coverslip covers all the tissue.

`

The label does not cover any tissue.

The example below shows minimum distances from the side and bottom edges of a 26mm x 76mm slide.
≥ 0.77 mm≥ 0.31 mm≥ 1.61 mm

For more details on slide specifications, see “Slide Types Supported” on page 25.

Fixing Slide Preparation Errors
Some physical problems of a slide can be resolved by cleaning the slide with a lint-free cloth or trimming the sides with arazor blade. Permanent problems with a slide may require the preparation of a new slide.

Coverslips
Coverslips are required when using the Aperio GT 450. They must be fully-cured or have completed the on-board drying
process for an automated coverslipper, such as the Leica Biosystems HistoCore SPECTRA Coverslipper.
The Aperio GT 450 supports common industry glass or plastic coverslips used for 1-inch x 3-inch (2.54 cm x 7.62 cm) slides.
See the Aperio GT 450 Specifications for coverslip specifications. (Glass coverslips are recommended.)
The quality of the scan is affected by the condition of the coverslip.
`

Keep the mounting media that attaches the coverslip to the slide to a minimum. Excess mounting media makes it
hard for the tissue finder to distinguish between actual tissue and the mounting media.

`

Make sure there are no air pockets under the coverslip.

`

For best results, do not mark or write on the coverslip.

`

The coverslip must not hang over the side of the slide.

`

Make sure there is only one coverslip attached to the slide.

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Chapter 3: Slide Preparation`

Wipe the coverslip clean of dust and fingerprints before loading the slide in the scanner.

LabelsThe Aperio GT 450 supports common industry labels used for 1 x 3 inch slides. Improperly applied slide labels can cause
slides to jam.
Ensure the following:`

Do not apply multiple labels to the same slide—this can cause the slide to exceed the specification for slide
thickness. See the Aperio GT 450 Specifications for slide specifications.

`Labels do not extend past the edges of the slide and that they do not cover any tissue.

`

Labels are not underneath coverslips.

`

Labels are firmly attached.

`

Do not apply labels to the bottom surface of the slide.

`

Always load slides into the rack with the label facing out and up, as “Load Slides in the Rack” on page 31.

The examples below show a slide with multiple labels that cause the slide to exceed the allowable maximum thickness, anda slide with the label peeling away:

BarcodesFor a list of supported barcodes, see “Barcodes Supported” on page 25. For best performance, we recommend using
barcode labels that have a white background and black ink.
Improperly applied slide barcodes can cause slides to jam. Ensure the following:

28`

Barcode labels meet the same application requirements as slide labels. See “Labels” on page 28.

`

Apply barcode labels to the slide label region.

`

Ensure a minimum of 0.5 mm between each side of the barcode and the edge of the label.

`

Apply only one barcode label per slide.

`

Use only supported barcode labels. (See “Barcodes Supported” on page 25.)`

Use only high-quality printed barcode labels.

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Scan Slides

This chapter shows you how to scan slides.

Scanning Concepts
This section provides basic concepts on the scanning workflow and the carousel rotation features. We recommend
reviewing this section before using the scanner.

Scan Workflow
The Aperio GT 450 is a continuous load scanner. You can continually load new racks and unload completed racks, as shown
below:
Scanner on

Rack rotatedautomatically

Slides loaded

Scanning starts

If manual image check
fails, re-scan slide

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Rack loaded

Scan and image auto-QC complete

Perform manual image
check

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Chapter 4: Scan Slides

Carousel Rotation
Review the following information to understand the carousel rotation and safety features.
The carousel automatically rotates after you load racks:
Racks loaded

10-second timer before
carousel rotates:

Safety light curtain
1. Racks rotated to scan
area in order loaded

2. Maximum empty rack slots
rotated to front

WARNING: Keep hands away from the carousel and pinch point areas when
the carousel is rotating.

An infrared safety lightcurtain detects objects inthe loading area:

If an object breaks thelight curtain:y the carousel does not
rotatey the 10-second timer
starts again

AVERTISSEMENT: Garder les mains éloignées du carrousel et des zones depoints de prise quand le carrousel est en rotation.

Log In and Log Out of the ScannerYour Lab Administrator sets up the scanner pass codes and timeout period for your lab using the Aperio GT 450 SAM client
application software. You need to log into the scanner to interact with the touchscreen interface. You do not need to log in
to load and unload racks for scanning.
As a security measure, the scanner logs you out after a period of time and requires you to enter the passcode.
1. From the touchscreen interface, tap Login.

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2. When the keypad screen appears, enter your 5-digit passcode.

To log out:
Logging out locks the touchscreen interface until a valid passcode is entered.
1. From the touchscreen interface, tap Logout.

Load Slides in the Rack
For a successful scan, ensure you use the correct orientation of the slides and the rack.
To load slides in the rack:
1. Position the rack so the Leica logo and “SIDE UP” facesup.
2. Insert each slide as shown with the label facing out andup.
3. Push each slide into the rack until the slide touches theback of the rack, ensuring each slide is fully inserted into
the rack.

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Load Racks in the Carousel
Slides are scanned in the order you load them.
1. Place the loaded rack into an open slot in the carousel.
2. Push the rack forward until it stops and you hear a click.

When the rack is fully inserted, there is a “click” sound, and the rack position on the Home screen turns light blue.

3. Continue loading racks as needed or until you fill the front six rack slots.
4. When you’ve finished loading the first set of racks:

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The first rack is rotated to the scan area.

`

Any empty rack slots are rotated to the loading area.
WARNING: Keep hands away from the carousel and pinch point areas when the
carousel is rotating.
AVERTISSEMENT: Garder les mains éloignées du carrousel et des zones de points
de prise quand le carrousel est en rotation.

5. When a rack is scanned successfully, the rack status turns green.

6. You can unload any completed racks and continue loading new racks.
If the completed rack is in the back of the carousel, you can rotate it to the rack loading area. See “Rotate a Rack to
the Rack Loading Area” on page 35.

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Chapter 4: Scan Slides

Priority Scanning
Use Priority to move a rack to the front of the scan queue. You can apply the Priority option on up to three racks at a time.
1. Tap the rack position.

The Priority option appears when you select the rack position.
2. Tap Priority.

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Chapter 4: Scan Slides

The Priority icon and scan queue number appear on the rack position:

The rack with the first level priority is moved to the top of the scan queue, and begins processing when the active
rack is complete. To view the rack scan order, see “View Rack Scan Order” on page 39.

i

To cancel a priority, you have to remove the rack.

Rotate a Rack to the Rack Loading Area
The main reason for using the rotate feature is to rotate a completed rack to the loading area. If you try to rotate the rackthat is currently being scanned, the system asks you for confirmation before continuing.
To rotate a rack to the rack loading area:
1. Tap the rack position.

The Rotate option appears when you select the rack position.

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Chapter 4: Scan Slides

2. Tap Rotate to rotate the rack to the loading area.

3. After the rack is rotated to the front, you can remove the rack. (The status light in the loading area blinks to indicate
the location of the rotated rack.)

Unload Racks from the Carousel
To unload racks from the carousel:
1. Make sure all the slides in the rack were successfully scanned (the rack status is green). If the rack status isorange, see “Rack Warnings” on page 40.
2. If the rack is in the loading area, carefully remove the rack from the rack slot.

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3. If the rack is not in the loading area, you can rotate it to the front:a. Tap the rack position.

ib. Tap Rotate.

If you try to rotate the rack that is currently being scanned, the system asks you for confirmation.

4. After the rack is rotated to the loading area, you can remove the rack.

Check Scan Status
This section describes the different ways to check scan status.
CAUTION: If you must remove a rack before all slides in the rack are scanned, note the rack andslide status. After removal, the rack’s scan status is no longer available.

Check Rack Status
To check the scan status of a rack:
1. Check the rack position status color against the Legend:

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Chapter 4: Scan Slides

2. The rack statuses are:
Empty

Rack is empty and available for use.

Complete

All slides in the rack scanned successfully and passed image QC. Scanned images are
saved to the specific location.

Scanning

The rack is currently being scanned.

Waiting to
Scan

The rack is loaded successfully and is waiting to scan.

Warning

There is an issue with the rack, or one or more slides in the rack. If you get a rack
warning, see page 40.

Priority

The rack is set for priority scanning. (See “Priority Scanning” on page 34.)

Rack Error on Current Scan
If there is an error on at least one side in the rack that is currently scanning, the rack position pulses blue and orange. See“Rack Warnings” on page 40.

View Slide Status for a Rack
To view the status for slides in a rack:
1. Tap the rack position on the touchscreen interface.
2. Tap the Rack View icon.

Slide status colors correspond to the Legend:
The slide is scanning.
The slide is waiting to scan.
The slide slot is empty.

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The scanned image was successfully transferred to the specific image location.
No scanned image created. (See page 40.)
The slide scanned successfully, passed image QC, and was transferred to the specific image location.
The slide has a scan warning. (See page 40.)

View Macro Images of Scanned Slides
To view a macro image of a scanned slide:1. Tap the rack position on the touchscreen interface.
2. Tap the Slide View icon to view individual images for the selected rack.
y Tap
and
to view other slides in the rack.
y If any tissue is outside the scan area, you can tap Scan Entire Slide to rescan
the whole slide.
y If there is an error scanning the slide, a message appears in the box. See “Slide
Errors and Solutions” on page 74.

View Rack Scan Order
1. Tap the Rack Order icon to show the scan order of the racks.
y Racks in the list appear in the scan order.
y The scan status is indicated for each rack.
y In the example, rack 3 is set as priority.

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Chapter 4: Scan Slides

Rack Warnings
A rack has a warning if the status color is orange or if the rack position is pulsating blue and orange.
To check the rack for errors:
1. Tap the rack position you want to check.
2. If there is a problem with the rack, a message appears similar to the example below.

3. Take note of the error code and message. For information and steps to resolve the rack error, go to “Rack Warnings
and Solutions” on page 72.
4. If there is a problem with one or more slides in the rack, a warning icon appears next to the slide in Rack View:
The scanner created an image, but there is an error.
An error prevented the scanner from creating an image.
In the example below, slide 7 in rack 1 has a warning.

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5. Tap the Slide View icon to view the macro image for the slide that has a warning.

6. Take note of the error code and message. For information and steps to resolve the error, go to “Slide Errors andSolutions” on page 74.

Scan Entire Slide for Whole Rack
This feature enables you to scan the full slide area for an entire rack of slides.
1. Load only the slides that require a full slide area scan in the rack.
2. Load the rack in the carousel.
3. Tap to select the rack position.

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Chapter 4: Scan Slides

4. Tap Settings, and then tap Scan Entire Slide.

The Scan Entire Slide option is selected, and the Settings icon

appears on the rack position:

Image Quality Control for Histotechnicians and Pathologists
After scanning slides, it is important to review the digital slides to make sure the images are of good quality. Ultimately, it is
up to the pathologist to look at digital slides to verify they are of sufficient quality to perform their task. In addition, scanner
operators should verify digital slide quality after scanning.
The quality criteria that should be verified by scanner operators are: 1) that the entire tissue sample has been scanned, and2) that the tissue is in focus.
Aperio scanners provide a macro image, a low resolution image of the entire slide, that also provides a green outline of thescanned image. Check that image for the quality criteria mentioned above.

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Re-scan any slides that failed to scan or failed the image quality review. For additional image quality assessment, refer tothe user guide for your viewer.

Scan Statistics
The Aperio GT 450 provides scan statistics, such as the number of slides or racks scanned per day, per week, etc. Tap Statsto view statistics for the Last 7 Days, Last 12 Months, or Lifetime by Year.

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5

MaintenanceThis chapter contains a maintenance schedule and procedures for maintaining your Aperio GT 450 scanner.
Call Leica Biosystems Technical Services if you cannot perform a maintenance routine or if you discover a problem with yourscanner.
Before performing maintenance, we recommend you watch the maintenance videos available on the touchscreen. See “HelpVideos and Guides” on page 24.

Maintenance ScheduleTo keep your Aperio GT 450 scanner in optimal working condition, follow these maintenance routines.

Frequency

Maintenance Task

Procedure

Daily(Recommended)

Restart the scanner.

“Restart the Scanner” on page 47.

Every Six Months

1. Clean the objective and Koehler“Clean the Objective and Koehler” on page 48

2. Clean the stage slide tray

“Clean the Stage Slide Tray” on page 51

3. Clean the carousel“Clean the Carousel” on page 52

4. Clean the slide racks“Clean the Slide Racks” on page 55

5. Clean the fan filter“Clean the Fan Filter” on page 53

6. Clean the touchscreen“Clean the Touchscreen” on page 56

7. Clean the scanner cover“Clean the Scanner Cover” on page 55

Schedule annual maintenance visit by
Leica Biosystems Technical Services.

Call Leica Biosystems Technical Services.

Once a year

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Customer Support contact information is provided
on page 2.

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Serial Number and Firmware Version
You need the serial number and current software version if you call Leica Biosystems Technical Support. Tap Maintenanceon the touchscreen interface to view the serial number, firmware version, and other system information.

Shut Down the Scanner
Use this procedure when you need to turn off the scanner.
1. From the touchscreen interface, tap Maintenance.

2. Tap Shut Down Scanner.
3. After the touchscreen goes dark, turn the scanner off using the On/Off switch.

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Chapter 5: MaintenanceOpen the Scanner Cover and Access the Interior Components
Complete these steps to access the inside of the scanner for maintenance or troubleshooting.

i

If you are performing maintenance on the scanner, you should first remove any racks from the carousel.
1. Shut down the scanner using the steps in “Shut Down the Scanner” on page 45.
2. Grasp the cover using the handhold indentations as shown in the following examples:

3. Slide the cover out until it reaches the point shown above.
4. Rotate the VPU latch 180 degrees to the open position, as shown below.

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5. Carefully pivot the VPU outward as shown:

6. You can now access the interior components of the scanner for maintenance or troubleshooting purposes.

Recommended Daily MaintenanceThis section provides the recommended daily maintenance procedures.

Restart the Scanner`Frequency: daily, and as needed to resolve a scanner issue.

Restarting the scanner initializes the controller and puts the camera, stage, and autoloader in their home positions.
CAUTION: Restarting the scanner with a slide on the stage may damage the slide.
1. Before restarting the scanner, ensure the following:`

All racks are unloaded from the carousel.

`

There are no slides scanning.

`

The system does not have any errors displayed.

2. From the touchscreen interface, tap Maintenance and tap Restart Scanner.

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Chapter 5: Maintenance

You can use the scanner again after initialization is complete and the Home screen appears.

Six Month MaintenanceThis section provides the six-month maintenance procedures. The purpose of the six month maintenance is to clean thescanner components.

Clean the Objective and Koehler`

Frequency: at least every six months.
CAUTION: When cleaning the scanner’s objective:y Do not remove the objective.
y Only use the recommended cleaning cloth and solution on the objective. Otherwise, thismay damage the objective and impact image quality.
y Do not drag anything (including lens paper) across the lens surface using a lot ofpressure. This may damage the lens.

Materials Needed

48`

Texwipe TX404 Absorbond Synthetic Wipers (lens cleaning wipes made of lint-free microfiber).

`Laboratory gloves.

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Chapter 5: MaintenanceTo clean the objective and Koehler:
1. Ensure the scanner is turned off. (For instructions, see “Turn the Scanner On and Off” on page 21.)
2. Open the cover and access the interior:a. Open scanner cover.

b. Rotate VPU latch.

c. Pivot out VPU

For detailed steps, see “Open the Scanner Cover and Access the Interior Components” on page 46.
3. The objective and Koehler shown below in blue:

Objective

Koehler

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Chapter 5: Maintenance

4. Manually position the slide stage so that you have clear access to the objective, as shown below.

CAUTION: The objective may move when you’re cleaning it.

5. Wear gloves when cleaning the objective.
6. Clean the objective using small circular motions with the recommended lint-free microfiber cloth.

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7. With a clean lint-free microfiber cloth, use the same technique described in step 6 to clean the Koehler. The
Koehler is located below the objective, as shown circled in white below.

8. If you are performing the entire six-month maintenance routine, continue to the next procedure.
9. If you are ready to close the scanner cover and restart the scanner, follow the steps in “Restart the Scanner After
Interior Maintenance” on page 56.

Clean the Stage Slide Tray`

Frequency: at least every six months.

To clean the stage slide tray:
1. If the scanner is on, shut down the scanner by following the steps in,“Shut Down the Scanner” on page 45.
2. Open the cover and access the interior.
a. Open scanner cover.

b. Rotate VPU latch.

c. Pivot out VPU

For detailed steps, see “Open the Scanner Cover and Access the Interior Components” on page 46.

CAUTION: The stage slide tray is not removable. Do not attempt to remove it.

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Chapter 5: Maintenance

3. Gently wipe the slide tray (shown in blue) from back to front with a lint-free cloth.

CAUTION: Be careful not to bump the LED or the objective (highlighted in red).

4. Wipe the slide tray again from back to front using a microfiber cloth.
5. If necessary, use a gentle solvent such as denatured alcohol to remove any remaining residue.
6. If you are performing the entire six-month maintenance routine, continue to the next procedure.
7. If you are ready to close the scanner cover and restart the scanner, follow the steps in “Restart the Scanner After
Interior Maintenance” on page 56.

Clean the Carousel`

Frequency: at least every six months.

To clean the carousel:
1. If the scanner is on, shut down the scanner by following the steps in,“Shut Down the Scanner” on page 45.
2. Remove all slide racks from the carousel.
3. Use a cotton swab soaked in a cleaning solvent, such as denatured alcohol, to thoroughly clean the carousel. Be
sure to clean inside the slide rack slots.

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CAUTION: When manually rotating the carousel to access the rack slots, grasp the area circled ingreen. Do not grasp the area circled in red.

4. Manually rotate the carousel to access the rear slide rack slots.
5. Repeat the cleaning steps until you have cleaned every slide rack slot.
6. If you are performing the entire six-month maintenance routine, continue to the next procedure.
7. If you are ready to close the scanner cover and restart the scanner, follow the steps in “Restart the Scanner After
Interior Maintenance” on page 56.

Clean the Fan Filter
The fan filter is located on the back of the scanner. You may need to pivot the scanner to access the fan filter.
`

Frequency: at least every six months.

To clean the fan filter:
1. If the scanner is on, shut down the scanner by following the steps in,“Shut Down the Scanner” on page 45.
2. Remove the fan filter by gripping the handle (shown in blue), and sliding it left, right, or up depending on yourscanner’s configuration.

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Chapter 5: Maintenance3. With the front of the filter up toward the faucet (as shown below), rinse the filter under warm water.

4. Shake off any excess water.
5. Dry the filter thoroughly with a clean cloth or a dryer.
CAUTION: Ensure the fan filter is completely dry before inserting it in the scanner.

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6. When the filter is completely dry, slide it back into position.

7. If you are performing the entire six-month maintenance routine, continue to the next procedure.
8. If you are ready to close the scanner cover and restart the scanner, follow the steps in “Restart the Scanner After
Interior Maintenance” on page 56.

Clean the Slide Racks`

Frequency: at least every six months.

To clean the slide racks:
1. Check the racks for any damage or accumulated glass dust and mounting media.
2. If a rack is damaged, replace it immediately.
3. Use compressed air or a cleaning solvent to clean all grooves in the rack, ensuring the racks are clean and smooth.
(We recommend Xylene cleaning solvent.)
4. If you are performing the entire six-month maintenance routine, continue to the next procedure.
5. If you are ready to close the scanner cover and restart the scanner, follow the steps in “Restart the Scanner After
Interior Maintenance” on page 56.

Clean the Scanner Cover`Frequency: at least every six months.

To clean the scanner cover:
1. Wipe the outer cover of the scanner with a damp cloth.
2. Immediately dry the cover using a dry cloth.

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Chapter 5: Maintenance

Clean the Touchscreen`

Frequency: at least every six months.

To clean the touchscreen:
1. Spray standard monitor cleaner directly on a clean non-scratch cloth. (Avoid spraying the touchscreen directly toensure that no liquid gets inside the scanner.)
2. Thoroughly wipe down the touchscreen with the cloth.
3. If you are ready to close the scanner cover and restart the scanner, follow the steps in “Restart the Scanner After
Interior Maintenance” on page 56.

Restart the Scanner After Interior MaintenanceFollow these steps to restart the scanner after you have accessed the scanner interior.
1. Pivot the VPU into the closed position.

2. Secure the VPU in place by rotating the VPU latch toward you 180 degrees.

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Chapter 5: Maintenance3. Slide the scanner cover back into position so that it is aligned with the rear latches and clicks closed.

4. Turn on the scanner.

Transporting or Moving the Aperio GT 450
If you need to relocate the scanner, contact Leica Biosystems Technical Services.

Long Term Storage
If you are not going to use the scanner for a considerable amount of time, shut it down and unplug it. To shut down thescanner, follow the steps in, “Shut Down the Scanner” on page 45.
If you want to store the scanner, contact Leica Biosystems Imaging Technical Services for assistance.

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6

TroubleshootingThis chapter contains information and instructions to help you resolve troubleshooting issues with yourscanner. For issues with the Scanner Administration Manager, see the Aperio GT 450 IT Manager and Lab
Administrator Guide.

Personal Protective Equipment
If you need to access the interior of the scanner while troubleshooting an issue, follow the policies and procedures of yourinstitution, including the use personal protective equipment (PPE).

Red Blinking Lights on Carousel
If the lights in front of the carousel are blinking red, the scanner needs attention. If there is an issue with a rack in the rackloading area, the light below that rack position blinks red. See the next section for more details on resolving scanner issues.

How to Use the Troubleshooting Steps
The following table describes how to find the appropriate troubleshooting section:

Type of issue:

Scanner status:

An error message box similar to this example appears on the Until the error istouchscreen interface:resolved:

58

Steps to resolve the issue:
1. You can tap thetominimize the message box.

y You cannot
interact with thecarouselThis enables you to view therack status and access theHelp resources.

y The scanner
cannot continue
scanning.

2. See “Error Codes andSolutions” on page 61 forsteps to resolve the specificerror.

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Type of issue:

Scanner status:

Steps to resolve the issue:

A rack warning and error message similar to this example
appears on the touchscreen interface:

There is a problem
with the rack or one
or more slides in therack.

See “Rack Warnings andSolutions” on page 72 for steps
to resolve the specific error.

The scanner cancontinue scanning
other racks or slides.

No message or warning appears on the touchscreeninterface, but there is a problem with the scanner. For
example, the scanner does not power up when you turn iton.

Varies with thesituation.

See “Symptoms and Solutions”
on page 79.

Perform a Safe Restart After an Error
Some procedures in this chapter ask you to restart the scanner. Restarting the scanner initializes the controller and puts
the stage and autoloader in their home positions. Before restarting the scanner, you need to ensure there is no slide on thestage.
Follow these steps to safely restart the scanner after an error:
1. Open the cover and access the interior:a. Open scanner cover.

b. Rotate the VPU latch.

c. Pivot out VPU

For detailed steps, see “Open the Scanner Cover and Access the Interior Components” on page 46.

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2. Check if there is a slide on the stage or partially on the stage.

CAUTION: Restarting the scanner with a slide on the stage may damage the slide.
3. If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components.
4. Return the pusher to the retracted (safe) position.
a. Align the pusher teeth with the slide stage
grooves:b. Slide the stage to the rear of the scanner, as shown:c. Slide the pusher to the front of the scanner, as shown in the rightmost image below. Hold the pusher in oneof the areas circled below. Avoid touching the LED and objective.

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5. Close the scanner cover:a. Pivot the VPU into
place.

b. Rotate the VPU latch.

c. Slide the cover until it clicks
closed.

For detailed steps, see “Restart the Scanner After Interior Maintenance” on page 56.
6. Tap Maintenance on the touchscreen interface, then tap Restart Scanner. Wait for the scanner to complete therestart process.

Error Codes and Solutions
If an error box appears on the touchscreen, you must resolve the issue before the scanner can continue scanning. Thissection contains error codes and messages with troubleshooting steps.

1000: Internal error`

Cause: An unexpected event occurred in the system that prevents it from continuing operation.

Follow these steps:
1. Open the cover and access the interior:a. Open scanner cover.

b. Rotate the VPU latch.

c. Pivot out VPU

For detailed steps, see “Open the Scanner Cover and Access the Interior Components” on page 46.
2. Take photos of any obstructions. Leica Biosystems Technical Services may request the photos if you need additional
assistance after completing the steps below.

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3. Check if there is a slide on the stage or partially on the stage.

CAUTION: Restarting the scanner with a slide on the stage may damage the slide.
4. If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components.
5. If the pusher is extended, return the pusher to the safe position.
a. Align the pusher teeth with the slide stage
grooves:b. Slide the stage to the rear of the scanner, as shown:c. Slide the pusher to the front of the scanner, as shown in the rightmost image below. Hold the pusher in oneof the areas circled below. Avoid touching the LED and objective.

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6. Close the scanner cover:a. Pivot the VPU into
place.

b. Rotate the VPU latch.

c. Slide the cover until it clicks
closed.

For detailed steps, see “Restart the Scanner After Interior Maintenance” on page 56.
7. Tap Restart Scanner on the touch screen interface, and wait for the scanner to complete the restart process.
8. If the issue persists, call Leica Biosystems Technical Services.

1001: Scanner cannot initialize`

Cause: The scanner cannot complete the initialization process.

Follow these steps:
1. Open the cover and access the interior:a. Open scanner cover.

b. Rotate the VPU latch.

c. Pivot out VPU

For detailed steps, see “Open the Scanner Cover and Access the Interior Components” on page 46.
2. Check if there is a slide on the stage or partially on the stage.

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CAUTION: Restarting the scanner with a slide on the stage may damage the slide.
3. If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components.
4. If the pusher is extended, return the pusher to the safe position.
a. Align the pusher teeth with the slide stage
grooves:b. Slide the stage to the rear of the scanner, as shown:c. Slide the pusher to the front of the scanner, as shown in the rightmost image below. Hold the pusher in oneof the areas circled below. Avoid touching the LED and objective.

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5. Close the scanner cover:a. Pivot the VPU into
place.

b. Rotate the VPU latch.

c. Slide the cover until it clicks
closed.

For detailed steps, see “Restart the Scanner After Interior Maintenance” on page 56.
6. Shut down the scanner using the steps in, “Shut Down the Scanner” on page 45.
7. Turn on the scanner, and wait for the initialization process to complete.
8. If the issue persists, call Leica Biosystems Technical Services.

1002: Carousel cannot rotate`

Cause: Something is blocking the light curtain.

Follow these steps in order until the issue is resolved and the error message closes:
1. Check the rack loading area and pinch points for any unexpected object.
Rack loading area with light curtain highlighted:

Pinch points on either edge of rack loading area:

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2. Ensure the racks in the rack loading area are inserted correctly:
Leica logo facing out and up:

Rack inserted fully into the rack slot:

3. When you insert the rack, ensure the rack position and the rack status light turns light blue (waiting to scan):Empty rack slot:

Rack inserted and waiting to scan:

4. Ensure all the slides are fully inserted so that they touch the back of the rack.

5. If there are no obstructions and the carousel still does not rotate, restart the scanner. See “Perform a Safe Restart
After an Error” on page 59.
6. If the issue persists, contact Leica Biosystems Technical Services.

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1003: Carousel cannot rotate. Carousel pinch point obstructed.
`

Cause: There is an obstruction at a pinch point.

Follow these steps in order until the issue is resolved and the error message closes:
1. Check the rack loading area and pinch points for any unexpected object that may break the light curtain.
Rack loading area with light curtain highlighted:

Pinch points on either edge of rack loading area:

2. If there are no obstructions and the carousel still does not rotate, restart the scanner by following the steps in,“Perform a Safe Restart After an Error” on page 59.
3. If the issue persists, contact Leica Biosystems Technical Services.

1007: Internal storage full. Cannot send images to DICOM converter.
`

Cause: If the internal storage is full, the system cannot send the images to the DICOM converter.

The Lab Administrator should perform these steps:
1. Ensure the LAN cables are connected at the scanner LAN port and at the SAM server.
2. Run network diagnostics.
3. Ensure the DICOM server is running. Restart the DICOM server if necessary.
4. If issue persists, contact Leica Biosystems Technical Services.

2000: Slide handling error at slide stage, rack, or pusher.
`

Cause: There is an obstruction at the slide stage, rack, or pusher.

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Follow these steps in order until the issue is resolved:
1. Open the cover and access the interior:a. Open scanner cover.

b. Rotate the VPU latch.

c. Pivot out VPU

For detailed steps, see “Open the Scanner Cover and Access the Interior Components” on page 46.
2. Take photos of the obstruction. Leica Biosystems Technical Services may request the photos if you need additional
assistance after completing the steps below.
3. Check if there is a slide on the stage or partially on the stage.

CAUTION: Restarting the scanner with a slide on the stage may damage the slide.
4. If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components.
5. Check the slide stage, rack, and pusher area for an obstruction.

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6. If possible, carefully remove the slide that is causing the obstruction.
WARNING: Do not attempt to recover broken slides. Call Leica Biosystems Technical Services.
AVERTISSEMENT: Ne pas tenter de récupérer les lames brisées. Appeler les Services
Techniques de Leica Biosystems.
7. Check the slide for preparation issues, such as over-hanging coverslips and label issues.
`

If there are no obvious preparation issues, re-insert the slide in an available rack after you restart the scanner.

`

If there are slide preparation issues, correct the slide issues before rescanning.

8. If the pusher is extended, return the pusher to the safe position.
a. Align the pusher teeth with the slide stage
grooves:b. Slide the stage to the rear of the scanner, as shown:c. Slide the pusher to the front of the scanner, as shown in the rightmost image below. Hold the pusher in oneof the areas circled below. Avoid touching the LED and objective.

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9. Close the scanner cover:a. Pivot the VPU into
place.

b. Rotate the VPU latch.

c. Slide the cover until it clicks
closed.

For detailed steps, see “Restart the Scanner After Interior Maintenance” on page 56.
10. Tap Restart Scanner on the touch screen interface, and wait for the scanner to complete the restart process.
11. If the issue persists, contact Leica Biosystems Technical Services.

2001: Slide handling error at rack gripper, lift, or carousel.
`

Cause: There is an obstruction near the rack gripper, lift, or carousel.

Follow these steps until the issue is resolved:
1. Open the cover and access the interior:a. Open scanner cover.

b. Rotate the VPU latch.

c. Pivot out VPU

For detailed steps, see “Open the Scanner Cover and Access the Interior Components” on page 46.
2. Take photos of the obstruction.
WARNING: Do not attempt to recover broken slides. Call Leica Biosystems Technical
Services.
AVERTISSEMENT: Ne pas tenter de récupérer les lames brisées. Appeler les Services
Techniques de Leica Biosystems.

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3. Check if there is a slide on the stage or partially on the stage.

CAUTION: Restarting the scanner with a slide on the stage may damage the slide.
4. If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components.
5. Check the rack gripper, lift, and carousel area for an obstruction.

6. Close the scanner cover:a. Pivot the VPU into place.

b. Rotate the VPU latch.

c. Slide the cover until it clicks
closed.

For detailed steps, see “Restart the Scanner After Interior Maintenance” on page 56.
7. Contact Leica Biosystems Technical Services.

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9000: Scanner cover is open`

The scanner cover is not closed completely.

Follow these steps until the issue is resolved:
1. Slide the cover to the closed position, making sure the cover aligns with rear latches and clicks closed:

Rack Warnings and Solutions
Rack warnings indicate a problem with the rack or one or more slides in the rack. The scanner can continue scanning when
there is a rack warning.
CAUTION: If you need to remove a rack before all slides in the rack are scanned, first take note of therack and slide status. After you remove a rack, the rack’s scan status is no longer available on the
Home screen.

1005: Cannot process rack.
`72

Cause: There is an issue with a rack that prevents scanning.

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Follow these steps in order until the issue is resolved:
1. Rotate the rack to the rack loading area. (See “Rotate a Rack to the Rack Loading Area” on page 35.)
2. Ensure the rack is inserted correctly:
Leica logo facing out and up:

Rack inserted fully into the carousel:

3. Remove the rack and check the following:y

You are using a supported rack. (See “Slide Racks Supported” on page 25.)y

The rack is not damaged or modified.

4. Verify the error only occurs on one rack.
y

If the error only occurs on one rack, go to step 4.

y

If the error occurs on multiple racks, contact Leica Biosystems Technical Services.

5. If you verified the rack is a supported and undamaged rack, reinsert it into the carousel for scanning.
6. If the scanner still cannot process the rack, attempt to restart the scanner by following the steps in “Perform a Safe
Restart After an Error” on page 59.
7. If the issue persists, remove the rack and contact Leica Biosystems Technical Services.

1006: Cannot process one or more slides in rack.
`

Cause: There is an issue with one or more slides in the rack.

Follow these steps in order until the issue is resolved:
1. Tap the rack position that has the error, and tap Rack View to identify which slides have the error.
2. Tap Slide View to view the macro image of the slide, and check the following:
3. Go to the section that matches the slide error:`“Tilted Slide(s)” on page 74`“No Barcode” on page 75`“No Tissue” on page 75

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Chapter 6: Troubleshooting`“No Macro Focus” on page 75`“Image Quality” on page 76`“Image Transfer Error – Pending Retry” on page 77`“Aborted” on page 77

4. If the issue persists after following the relevant procedure, keep the slide available for inspection and contact Leica
Biosystems Technical Services.

Slide Errors and Solutions
If there is an issue scanning a slide, one of the following error messages appears in Slide View. Follow the steps in orderuntil the issue is resolved. If you follow the steps and the issue persists, contact Leica Biosystems Technical Services.

i

You can temporarily hide some error messages by tapping the x in the top-right corner. This enables you to viewthe macro image.

Tilted Slide(s)`Cause: The slide is tilted between two or more slots in the rack and cannot be scanned.

Follow these steps in order:
1. Remove the rack from the carousel, and locate the tilted slide:

2. Insert the slide in a new rack for scanning, ensuring it sits level horizontally in one slot.
3. Insert the rack to an empty rack slot for scanning.

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Chapter 6: Troubleshooting

No Barcode`

Cause: The scanner does not detect a barcode on the slide.

Follow these steps in order until the issue is resolved:
1. Check the slides and rack are inserted correctly:`

Slide label facing out and up.

`

Leica logo facing out and up.

`

See also, “Load Slides in the Rack” on page 31.

2. Ensure barcodes meet specifications. See “Barcodes Supported” on page 25.
3. Ensure barcodes meet minimum quality requirements. See “Barcodes” on page 28.
4. If the issue persists, call Leica Biosystems Technical Services.

No Tissue`

Cause: The scanner does not detect tissue on the slide.

Follow these steps in order until the issue is resolved:
1. On the touchscreen interface, tap the rack position that has the error.
2. Tap the Slide View icon to view the macro image of the slide.

3. Tap the error message to temporarily hide it.
4. Verify there is tissue on the slide.
5. Take note of the slide number.
6. Rotate and remove the rack to access the slide.
7. Remove the slide from the rack, and then clean the slide.
8. Re-insert the slide into a new rack and scan it again.
9. If the issue persists, check for slide preparation errors. See “Slide Preparation” on page 26.
10. If the issue persists after completing the previous steps, call Leica Biosystems Technical Services.

No Macro Focus`

Cause: The scanner’s camera cannot focus on the tissue.

Follow these steps in order until the issue is resolved:
1. Check for slide loading issues:`

Slide orientation is correct with the specimen-side facing up. (See “Load Slides in the Rack” on page 31.)`

Slide tray is clean.

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Chapter 6: Troubleshooting

2. Check the stain quality.
3. Check that the slide and coverslip thickness are meet the requirements. See the Aperio GT 450 Specifications.
4. Check for common slide preparation issues:`

Coverslip is not missing or overhanging.

`Labels are not overhanging or on wrong side.

`

There is only one label applied to the slide.

`

Slide is clean.

5. If every slide has the same error, or if the issue persists after following the previous steps, call Leica Biosystems
Technical Services.

Image Quality`

Cause: The scanner’s Auto-Image QC feature detected an image quality issue.

Follow these steps in order until the issue is resolved:
1. Review the scanned slide image in Aperio ImageScope.
2. Check for common slide preparation issues:`

Coverslip is not missing or overhanging.

`Labels are not overhanging or on wrong side.

`

There is only one label applied to the slide.

`

Slide is clean.

3. Scan the slide again. When the scan is finished, do not remove the rack from the scanner.
4. Review the newly scanned slide image in Aperio ImageScope.
5. If the issue persists, display the slide in Slide View, and tap Scan Entire Slide. (To scan the whole area for a rackof slides, see “Scan Entire Slide for Whole Rack” on page 41.)
6. Check if other slides are in focus.
7. If all slides are out of focus, clean the objective. (See the “Clean the Objective and Koehler” on page 48.)
8. If the issue persists after following the previous steps, call Leica Biosystems Technical Services.

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Chapter 6: Troubleshooting

Image Transfer Error – Pending Retry`

Cause: The scanner cannot transfer the scanned image to the image storage location.

Follow the appropriate steps below:
Error appears on some slides:

Error appears on all slides:

The system often resolves the issue without intervention.

The Lab Administrator should:

1. If all slides in the rack are finished scanning, remove
the rack.

1. Check connectivity from the scanner to the DICOM
server, and the DICOM server to your site’s image
storage location.

2. Check for the image in eSlide Manager.
3. If necessary, rescan only the slides that are missing
from eSlide Manager.

2. Check if your site’s image storage location is full.
3. If the issue persists, consult with your organization’s
IT professionals prior to calling Leica Biosystems
Technical Services.

Aborted`Cause: The scanner cannot scan the slide.

Follow these steps in order until the issue is resolved.
1. If the “Aborted” message appears on all slides, go to “Aborted Message Appears on All Slides” on page 78. If
the message appears on one or some slides, continue to the next step.
2. Check for damage or common slide preparation issues:`

Coverslip is not missing or overhanging.

`Labels are not overhanging or on wrong side.

`

There is only one label applied to the slide.

`

There is only one coverslip applied to the slide.

`

Slide is clean.

3. Clean the slide.
4. Insert the slide into a different rack and scan it again.
5. If the issue persists after following the previous steps, call Leica Biosystems Technical Services.

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Chapter 6: Troubleshooting

Aborted Message Appears on All Slides
Follow these steps in order until the issue is resolved:
1. Remove any completed slide racks from the carousel.
2. Open the cover and access the interior:a. Open scanner cover.

b. Rotate the VPU latch.

c. Pivot out VPU

For detailed steps, see “Open the Scanner Cover and Access the Interior Components” on page 46.
3. Check if there is a slide on the stage or partially on the stage.

CAUTION: Restarting the scanner with a slide on the stage may damage the slide.
4. If there is a slide on the stage, carefully remove it from the stage without touching the surrounding components.
5. Close the scanner cover:a. Pivot the VPU into place.

b. Rotate the VPU latch.

c. Slide the cover until it clicks
closed.

For detailed steps, see “Restart the Scanner After Interior Maintenance” on page 56.

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Chapter 6: Troubleshooting

6. Shut down the scanner by tapping Maintenance, then tapping Shut Down Scanner.
7. When touchscreen is dark, turn the scanner off using the On/Off switch.
8. Turn the scanner back on using the On/Off switch.
9. Allow the scanner to scan any remaining racks.
10. If the issue persists, call Leica Biosystems Technical Services.

Symptoms and Solutions
This section contains troubleshooting information organized by symptom for scanner issues that do not have an error
message or code.

Scanner does not power on
1. Ensure the scanner is turned on.
2. If you are using an optional uninterruptible power supply (UPS), ensure it is turned on.
3. Check the connections to wall outlet, and the Ethernet cable that attaches to the back of the scanner.
4. Ensure there is power available where the scanner is plugged in.
5. Verify there is a network connection to the device.
6. Ensure the main scanner cover is fully closed.
7. If the issue persists, call Leica Biosystems Technical Services.

Touchscreen does not respond to touch
1. Shut down the scanner by following the steps in, “Shut Down the Scanner” on page 45.
2. Turn the scanner on.
3. If the issue persists, contact Leica Biosystems Technical Services with details.

Touchscreen is black
1. Shut down the scanner by following the steps in, “Shut Down the Scanner” on page 45.
2. Turn the scanner on.
3. If the issue persists, contact Leica Biosystems Technical Services with details.

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Chapter 6: Troubleshooting

Slides are broken inside the scanner
WARNING: Do not attempt to recover broken slides. Call Leica Biosystems Technical Services.
AVERTISSEMENT: Ne pas tenter de récupérer les lames brisées. Appeler les Services Techniques deLeica Biosystems.
1. Take photos of the location of the damage. Leica Biosystems Technical Services may request the photos whenproviding assistance.

Internet connection lost
The Aperio GT 450 must be connected to the Scanner Administration Manager (SAM) through your Local Area Network tofunction. If that connection is lost, you see:

You can attempt to re-establish network connection by typing the IP address of the SAM server. (Contact your IT staff forthis information.) If this does not establish network connection, contact your IT staff for assistance.

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IndexSymbolsE

40x 24

electromagnetic warnings 13
error codes, solutions for 61
rack warnings 72
error message 58

A
add racks 32
add slides to rack 31
Auto-Image QC 24

Bbarcodes 28
barcodes supported 25
blinking lights, red 58
broken slides, recover 80

Ccannot process rack 72
capacity, slide 25
carousel 20
clean 52
lights 58
carousel cannot rotate 65
clean objective 48
compliance specifications 14
continuous load 24
cover. See scanner cover
cover, open and close 46
coverslips 25, 27

Ddaily maintenance 47
DICOM 24
DICOM converter error 67

Ffan filter
clean 53
remove 53
FCC compliance 14

Iimage quality check, automatic 24
image types, supported 24
initialization error 63
insert racks 32
insert slides 31
installation 15
instrument warnings 14
internal storage full 67

Llabels, requirements 28
labels, slides 28
legend 22
light curtain 21
load racks in scanner 32
load slides in rack 31

Mmagnification, scan 24

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81Indexmaintenancecleaning slide tray 52
daily 47
schedule 44
six-month 48
manual image quality check 42
messagesrack issues 59
scanner error 58
slide issues 59
move scanner 57

Nnumber of slides 25

Oobjective
clean 48
location 49
on/off switch 21
open cover 46
optionspriority 34
orange status 40
overview, scanner 20
overview, touchscreen interface 21

Ppersonal protective equipment 58
pinch point obstruction error 67
power on problem 79
PPE 58preparing slides 26
priority scan 34
pusher, safe position 56

Q
quality check, image 24

R
rackcheck status 37
load in scanner 32

82

load slides in 31
unload 36
warning 40
rack order 39
rack process error 72
racks
clean 55
rack view, display 38
rack warning 59
current scan 38
red blinking lights 58
regulatory compliance 14
remove rack 36
reports, scan 43
restart scanner
after an error 59
daily maintenance 47

Ssafety instructions 16
safety light curtain 21
scan entire slide
single slide 39
whole rack 41
scan magnification 24
scannerclose cover 56
move 57
open cover 46
restart 47
restart after maintenance 56
service life 15
storage 57
Scanner Administration Manager 24
scanner coverclean 55
scanner not powered on 79
scanner safety instructions 15, 16
scan statistics 43
scan workflow 29
sensors, light 21
service life 15

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Indexshut down scanner 45
six-month maintenanceclean carousel 52
clean fan filter 53
clean objective 48
clean scanner cover 55
clean slide racks 55
clean stage slide tray 51
clean touchscreen 56
slide capacity 25
slide handling error 67, 70
slide labels, requirements 28
slide preparation 26
coverslips 27
fix errors 27
labels 28
slide racks
clean 55
slides, broken 80
slides, load in rack 31
slide staining requirements 26
slide types 25
slide view 39
specifications
regulatory compliance 14
stage slide tray, clean 51
staining 26
staining requirements 26
statistics, scan 43
status legend 22
status, rackdescriptions 37
status, slide 38
storage, scanner 57
SVS files 24

troubleshooting 58
error codes 61
error messages 58, 59
how to use steps 58
lost internet connection 80
rack warnings 72
symptoms 72
touchscreen 79
turn on scanner 21

U
UI. See touchscreen
unload racks 36
unload slides 36
user guides, list of 13
user interface 21. See touchscreen

V
VPU
close 56
VPU, open 46, 47

W
warnings
rack 40, 72
warning symbols 13
whole slide scan. See scan entire slide
workflow, scan 29

Ttissue preparation 26
touchscreen
clean 56
touchscreen interface overview 21
touchscreen problem 79

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