Aperio GT 450
Quick Reference Guide
For research use only. Not for use in diagnostic procedures.

MAN-0392, Revision C | 25 October 2019

Aperio GT 450 Quick Reference Guide
Copyright NoticeÌÌCopyright © 2019 Leica Biosystems Imaging, Inc. All Rights Reserved. LEICA and the Leica logo are registered trademarks of Leica Microsystems IR GmbH.
Aperio is a trademark of the Leica Biosystems group of companies in the USA and optionally in other countries. Other logos, product and/or company names
might be trademarks of their respective owners.

Contact Information – Leica Biosystems Imaging, Inc.
Headquarters
Leica Biosystems Imaging, Inc.
1360 Park Center Drive
Vista, CA 92081
USA
Tel: +1 (866) 478-4111 (toll free)Direct International Tel: +1 (760) 539-1100

Customer Support

General Information

US/Canada Tel: +1 (844) 534-2262 (toll free)Direct International Tel: +1 (760) 539-1150

US/Canada Tel: +1 (866) 478-4111 (toll free)Direct International Tel: +1 (760) 539-1100

US/Canada/Worldwide Email:
TechServices@LeicaBiosystems.com

Email: ePathology@LeicaBiosystems.com

Aperio GT 450 Quick Reference Guide, Revision C

Contents
1. INTRODUCTION................................................. 5
Load Racks in the Carousel................................. 14
Unload Racks from the Carousel ..............................15
Help Videos........................................................... 5
Priority Scanning................................................. 16
User Guides........................................................... 5
Scan Status......................................................... 17
Scanner Overview................................................. 6
Scan Statistics.................................................... 18
Touchscreen Interface Overview.......................... 7
Rack View........................................................... 19
Scanning Workflow............................................... 8
Slide View........................................................... 20
Carousel Rotation................................................. 9
Rack Order........................................................... 20
Key Concepts...................................................... 10
Rack Warnings.................................................... 21
Icon Glossary....................................................... 11
Scan Entire Slide for Whole Rack....................... 22
2. SLIDE PREPARATION CHECKLISTS.............. 12
Slides.................................................................. 12 4. MAINTENANCE................................................ 23
Maintenance Checklists..................................... 23
Coverslips............................................................ 12
Daily (recommended).......................................... 23
Slide Labels......................................................... 12
Six Month............................................................ 23
Barcodes............................................................. 12
Yearly.................................................................. 23
3. SCAN SLIDES................................................... 13
Restart the Scanner............................................ 24
Load Slides in the Rack....................................... 13
Contents | 3

Aperio GT 450 Quick Reference Guide, Revision C

Shut Down the Scanner...................................... 24
5. TROUBLESHOOTING....................................... 25
Additional Resources.......................................... 25
Serial Number and Firmware Version................ 25
Open and Close the Scanner Cover.................... 26
Return Pusher to Safe Position........................... 27
Safe Restart After an Error................................. 28
Errors and Solutions............................................ 29
Broken Slides Inside the Scanner....................... 42
6. INDEX................................................................. 43

4 | Contents

Aperio GT 450 Quick Reference Guide, Revision C

Introduction
This guide provides basic steps for using the Aperio GT 450 digital slide scanner. See below for additional resources.
Please watch the videos (accessible from the touchscreen interface) before working with the scanner.

Help Videos

User Guides

Access training videos and this guide from the touchscreen:

In addition to this guide, the following guides are available
on the Leica Biosystems website:`` Aperio GT 450 User’s Guide`` Aperio GT 450 IT Manager and Lab Administrator
Guide`` Aperio GT 450 Specifications

Introduction | 5

Aperio GT 450 Quick Reference Guide, Revision C

Scanner Overview
Front

Back

Fan filter
Touchscreeninterface

CarouselStatus lights (6)On/off switch
Rack loading area (front 6)

6 | Introduction

Power / network connections

Aperio GT 450 Quick Reference Guide, Revision C

Touchscreen Interface Overview
The example below shows the Home screen.
Help videos and guides

Log in to the scanner

Maintenance area
Scanner statistics
Home screen(carousel view)

Rack view
Slide view
Rack order
Carousel and rack status

Selected rack
Rack status legend
Rack actions

Rack settings

Home screen (carousel view), scanner statistics, help videos and guides appear here

Slide view, rack order,
rack view appear here

Introduction | 7

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Scanning Workflow
This workflow shows the scanning process for one rack of slides. For step-by-step instructions, go to “Scan Slides” on page 13.
Scanner on

Rack rotatedautomatically

8 | Introduction

Slides loaded

Scanning starts

Rack loaded

Scan and Image QC completeAperio GT 450 Quick Reference Guide, Revision C

Carousel Rotation
The carousel automatically rotates after you load racks:
Racks loaded

10-second timer before
carousel rotates:

Safety light curtain
1. Racks rotated to scan
area in order loaded

2. Maximum empty rack slots
rotated to front

WARNING: Keep hands away from the carousel and pinch point areas when
the carousel is rotating.

An infrared safety lightcurtain detects objects inthe loading area:

If an object breaks thelight curtain:
yythe carousel does not
rotateyythe 10-second timer
starts again

AVERTISSEMENT: Garder les mains éloignées du carrousel et des zones depoints de prise quand le carrousel est en rotation.

Introduction | 9

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Key Concepts
Scanner settings

Your lab administrator defines the scan settings and sets the PIN codes.

Scanmagnification

The Aperio GT 450 always scans at 40x.

Rack scanning

All actions, such as setting priority, are applied to an entire slide rack (not individual slides).

Image quality

The Auto-Image QC feature automatically checks image quality as each slide is scanned. If the rack status isgreen, the scan and Image QC were successful for all slides in the rack.

Slide calibration

Each slide is calibrated when it’s scanned.

Continuous
loading

The Aperio GT 450 is a continuous loading scanner. You can continue to load new racks and unload
completed racks without interruption.

Automatic
carousel rotation

The carousel rotates the racks automatically. For details, see “Carousel Rotation” on page 9.

Visual statusindicators

The carousel view on the touchscreen and the status lights in front of the rack slots provide real-time status.

PIN code /scanner login

If the scanner asks for a PIN code, enter the 5-digit code provided by your Lab Administrator.

10 | Introduction

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Icon Glossary
The following icons appear on the touchscreen interface.
Carousel – Carousel view (Home screen).
Stats – Scanner statistics. (See page 18.)

Scan completed – In Rack View, indicates the slide
scanned successfully, passed image QC, and that theimage was saved to the specified image location. (Seepage 19.)

Help – Instructional videos and user guides.

Slide warning – In Rack View, alerts you of a slide
issue. (See page 21.)

Login – Scanner login, where you enter your 5-digit
PIN code.

Priority scan – On the rack position in the carouselview and in Rack View, indicates the rack’s priority
scan order, from 1 to 3. (See page 16.)

Logout – Lock the touchscreen interface.
Slide View – Macro image for the scanned slide.
(See page 20.)
Rack Order – Scan order for racks. (See page 20.)

Rotate – Appears on the touchscreen interface when
the carousel is rotating.
Rack Settings – In Carousel view, appears in thecenter. It also appears on the rack position when racksetting is applied. (See page 22.)

Rack View – Scan status for each slide in theselected rack. (See page 19.)

Introduction | 11

Aperio GT 450 Quick Reference Guide, Revision C

Slide Preparation Checklists
SlidesSlide Labels

Slides stained according to manufacturer’s instructions.

Only one label per slide.

Slides are clean. Wipe with clean lint-free cloth (no chemicalcleaners).

Labels do not extend past edges of the slide.

No dirt, fingerprints, markings, writing, extra mounting media,
broken slides, chips, or scratches.

Labels are not under the coverslip.

Slides are fully cured (not “wet”).
No mounting media around slide edges.
No tissue on coverslip edges or overlapping the label.
No stain residue around slide edge.

Coverslips
Fully cured coverslip is in place. You must use coverslips with
Aperio GT 450.
Minimal mounting media used.
No air pockets under coverslip.
Coverslip is not hanging over the side of the slide.
Only one coverslip per slide.

12 | Slide Preparation

Labels do not cover any tissue.
Labels are firmly attached.
Labels are not applied to the bottom surface of the slide.

BarcodesBarcode labels meet the same application requirements as slidelabels.
Barcode labels applied to the slide label region.
Only one barcode label per slide.
Use only supported barcode labels.
Use only high-quality printed barcode labels.

Aperio GT 450 Quick Reference Guide, Revision C

Scan Slides
This section contains instructions for scanning slides.

Load Slides in the Rack

3. Push the slides into the rack until they touch the back of
the rack.

1. Make sure the Leica logo and“SIDE UP” text on the rack isfacing up.

2. Make sure the slide labels arefacing out and up.

Scan Slides | 13

Aperio GT 450 Quick Reference Guide, Revision C

Load Racks in the Carousel
Slides are scanned in the order loaded.
1. Place the loaded rack into an open slot in the carousel in theorientation shown on page 13.
2. Push the rack forward until it stops.

When the rack is fully inserted, there is a “click” sound, andthe rack position on the Home screen turns light blue.
3. Continue loading racks as needed or until you fill the front-six
rack slots.
4. Wait for the carousel to rotate more empty rack slots to theloading area.

14 | Scan Slides

5. Continue loading racks as needed.
6. When a rack is scanned successfully, the rack status turns
green.

7. You can now remove the completed rack and load anotherrack.

Aperio GT 450 Quick Reference Guide, Revision C

Unload Racks from the Carousel
1. Remove the rack from the rack slot.

2. Tap Rotate to rotate the rack to the loading area.

To unload a rack that is not in the loading area:
1. Tap the rack position.

3. After the rack is rotated to the front, carefully remove therack.

Scan Slides | 15

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Priority Scanning
Use Priority to move one or more racks to the front of the scanqueue. You can apply the Priority option on up to three racks at atime.

2. Tap Priority.

1. Tap the rack position.

The Priority icon and scan queue number appear:

3. To view the rack scan order, see page 19.

i
16 | Scan Slides

To cancel a priority, you have to remove the rack.

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Scan Status
CAUTION: If you must remove a rack before all slides
in the rack are scanned, note the rack and slide status.
After removal, the rack’s scan status is no longer
available.

2. The rack statuses are:
Empty

Rack is empty and available for use.

Complete

All slides in the rack scanned successfully
and passed image QC. Scanned images are
saved to the specific location.

Scanning

The rack is currently being scanned.

Waiting to
Scan

The rack is loaded successfully and waiting
to scan.

Warning

There is an issue with one or more slides in
the rack. See page 21.

Priority

The rack is set for priority scanning (see page
16).

To check the scan status of a rack:
1. Check the rack position status color against the Legend:

Rack Error on Current Scan
If there is an error on at least one slide in the rack that iscurrently scanning, the rack position pulses blue and orange. See
page 21.

Scan Slides | 17

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Scan Statistics
To view scan statistics, tap Stats. To change the time frame of the statistics, tap the buttons above the graph.

18 | Scan Slides

Aperio GT 450 Quick Reference Guide, Revision C

Rack View
1. Tap the Rack View icon to show the scan status for each slide in the rack.
Slide status colors correspond to the Legend:
The slide is scanning
The slide is waiting to scan
The slide slot is empty.
The scanned image was successfullytransferred to the specific image location.
No scanned image was created. (See page21.)
The slide scanned successfully, passed image
QC, and was transferred to the specified image
location.
The slide has a warning. (See page 21.)

Scan Slides | 19

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Slide View

Rack Order

1. Select the rack by tapping it on the touchscreen.

1. Tap the Rack Order icon to show the scan order of theracks.

2. Tap the Slide View icon to individual images for theselected rack.
yyTap and to view otherslides in the rack.
yyIf any tissue is outside thescan area (the green box), you
can tap Scan Entire Slide torescan the whole slide.
yyIf there is an error scanning theslide, a message appears in thebox. (See page 21.).

20 | Scan Slides

yyRacks in the list appear in thescan order.
yyThe scan status is indicated foreach rack.
yyIn the example, rack 3 is set as
priority.

Aperio GT 450 Quick Reference Guide, Revision C

Rack Warnings
An orange rack position indicates a problem with the rack, or one
or more slides in the rack.
1. Tap to select the rack position with the warning.
2. Note the error message and number, and go to “Errors andSolutions” on page 29.

Rack issues:

Slide issues:
Orange warning
symbolin Rack View
indicates slide
error.

Error message andnumber appears.
Slide error
message andnumber appear
in Slide View.

Scan Slides | 21

Aperio GT 450 Quick Reference Guide, Revision C

Scan Entire Slide for Whole Rack
This feature enables you to scan the full slide area for a rack ofslides.
1. Load only the slides that require a full slide area scan in therack.
2. Load the rack in the carousel.
3. Tap to select the rack position.
4. Tap Settings, and then tap Scan Entire Slide.

22 | Scan Slides

5. The Scan Entire Slide option is selected, and the Settings
icon
appears on the rack position.

Aperio GT 450 Quick Reference Guide, Revision C

Maintenance
Maintenance Checklists
To maximize scanner performance and image quality, follow this recommended maintenance schedule.

Daily (recommended)
Restart the scanner. (Seepage 24.)Six Month
Clean the objective and Koehler
Clean the stage slide tray
Clean the carousel
Clean the slide racks
Clean the fan filter
Clean the touchscreen
Clean the scanner cover
The Six Month Maintenance procedures areprovided in the Aperio GT 450 User’s Guide“Maintenance” chapter.

YearlyLeica Biosystems Technical
Services annual maintenancevisit. (Contact Leica Biosystems
to schedule. See page 2 forcontact details.)

Maintenance | 23

Aperio GT 450 Quick Reference Guide, Revision C

Restart the Scanner

Shut Down the Scanner

Restart the scanner once daily for maintenance.

Use this procedure to safely turn off the scanner.

CAUTION: Restarting the scanner with a slide on thestage may damage the slide.

1. From the touchscreen interface, tap Maintenance.

1. Ensure all racks are unloaded, no slides are scanning, andthere are no errors displayed.
2. Tap Maintenance, then tap Restart Scanner.

2. Tap Shut Down Scanner.
3. After the touchscreen goes dark, turn the scanner off using
the On/Off switch.

You can use the scanner again after initialization is complete
and the Home screen appears.

24 | MaintenanceAperio GT 450 Quick Reference Guide, Revision C

TroubleshootingThis section provides some common troubleshooting issues and solutions. For a comprehensive list of troubleshooting
issues, see the Aperio GT 450 User’s Guide.

Additional Resources`` Aperio GT 450 User’s Guide contains a comprehensive “Troubleshooting” chapter.
`` The Maintenance Videos available from the touchscreen interface include common troubleshooting procedures. Tap
Maintenance, then tap Maintenance Videos.

Serial Number and Firmware Version
You need the serial number and current software version if you call Leica Biosystems Technical Support.
`` Tap Maintenance on the touchscreen to view the serial number, firmware version, and other system information.
`` The serial number and firmware version are also available from the Aperio GT 450 SAM Application Software. See the Aperio
GT 450 IT Manager and Lab Administrator Guide.

25 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Open and Close the Scanner CoverYou need to open the cover to perform certain maintenance and troubleshooting tasks. For detailed instructions on how to open andclose the scanner, see the user’s guide for the scanner.

Open the CoverClose the Cover

26 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Return Pusher to Safe Position
Follow these steps when the troubleshooting procedure asks you to return the pusher to the safe position.
1. Align the pusher teeth with the slide stage groves:

2. Slide the stage to the rear of the scanner:

3. Hold the pusher in one of the areas circled in blue, avoiding
the LED and objective.

4. Slide the pusher to the front of the scanner as shown.

27 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Safe Restart After an Error
Some procedures ask you to restart the scanner. Restarting the scanner initializes the controller and puts the stage and autoloader intheir home positions. Before restarting the scanner, you need to ensure there is no slide on the stage.
1. Open the cover and access the interior (see page 26).
2. Check if there is a slide on the stage orpartially on the stage.
3. Carefully remove the slide from thestage without touching the surrounding
components.

CAUTION: Restarting thescanner with a slide on thestage may damage the slide.

4. Return the pusher to the retracted (safe) position, as shown in “Return Pusher to Safe Position” on page 27.
5. Close the VPU and scanner cover (see page 26).
6. Tap Maintenance on the touchscreen interface, then tap Restart Scanner.

28 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Errors and Solutions
CAUTION: If you need to remove a rack before all slides in the rack are scanned, first take note of the rack and slide
status. After removal, the rack’s scan status is no longer available. If an error message is covering the rack and slide status,
minimize the error message by tapping thein the upper-right corner of the message box.

Error Code 1000
Internal Error
1. Open the cover and access the interior (see page 26).
2. Take photos of any obstructions for Leica Biosystems Technical Services.
3. Check if there is a slide on the stage orpartially on the stage.
4. Carefully remove the slide from thestage without touching the surrounding
components.

CAUTION: Restarting thescanner with a slide on thestage may damage the slide.

5. If the pusher is extended, return the pusher to the safe position as described in “Return Pusher to Safe Position” on page 27.
6. Close the VPU and scanner cover (see page 26).
7. Tap Restart Scanner, and wait for the scanner to complete the restart process.

29 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Error Code 1001
Scanner Cannot Initialize
1. Open the cover and access the interior (see page 26).
2. Check if there is a slide on the stage orpartially on the stage.
3. Carefully remove the slide from thestage without touching the surrounding
components.

CAUTION: Restarting thescanner with a slide on thestage may damage the slide.

4. If the pusher is extended, return the pusher to the safe position as described in “Return Pusher to Safe Position” on page 27.
5. Close the VPU and scanner cover (see page 26).
6. Shut down the scanner using the steps in, page 24.
7. Turn on the scanner, and wait for the initialization process to complete.

30 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Error Code 1002

Error Code 1003

Carousel cannot rotate.

Carousel cannot rotate. Carousel pinch point obstructed.

1. Check the rack loading area and pinch points for any
unexpected object:

1. Check the rack loading area and pinch points for any
unexpected object:

2. Ensure Leica logo faces up and out of the racks, and thatracks are inserted fully in the carousel.
3. Ensure all slides are fully inserted into the racks.
4. Check for other obstructions in the rack loading area.

2. If there are no obstructions and carousel still does not rotate,
follow the steps in, “Safe Restart After an Error” on page28.

5. If there are no obstructions and carousel still does not rotate,
follow the steps in, “Safe Restart After an Error” on page28.

31 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Error Code 1005

Error Code 1006

Cannot process rack

Cannot process one or more slides in rack

1. Ensure the rack is inserted correctly. (See page 14.)

1. Tap the rack that has the error, and tap Rack View to
identify which slides have the error.

2. Remove the rack and check:yyYou are using a supported rack.
yyThe rack is not damaged.
3. Verify the error only occurs on one rack. If the error occurs on
multiple racks, contact Leica Biosystems Technical Services.
4. If the rack is supported and undamaged, reinsert it into the
carousel for scanning.
5. If the scanner still cannot process the rack, contact Leica
Biosystems Technical Services.

32 | Troubleshooting

2. Tap Slide View to view the macro image of the slide, andcheck the following:
3. Go to “Slide Error Messages for Error Code 1006” on page
33 and follow the steps for the specific slide error.

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Slide Error Messages for Error Code 1006
After identifying which slide has the error, follow the appropriate steps below.

i

You can temporarily hide some error messages by tapping the x in the top-right corner.

Error Message

What to do

Tilted Slide

1. Remove the rack from the carousel.
2. Locate and remove the slide that is tilted between multiple rack slots.
3. Insert the slide in a new rack for scanning, ensuring it sits level horizontally in one slot.
4. Insert the rack to an empty rack slot for scanning.

No Barcode

1. Check that the slides and rack are inserted correctly.
2. Ensure barcodes meet specifications and the minimum quality requirements described in the scanner user
guide.

No Tissue

1. Verify there is tissue on the slide.
2. Take note of the slide number.
3. Rotate and remove the rack to access the slide.
4. Remove the slide from the rack, and then clean the slide.
5. Re-insert the slide into a new rack and scan it again.
6. If the issue persists, check for slide preparation errors.

33 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Error Message

What to do

No Macro
Focus

1. Check the slides and rack are inserted correctly. See page 13.
2. Check that the slide tray is clean.
3. Check the stain quality.
4. Check that the slide and coverslip meet the requirements described in the Aperio GT 450 Specifications.
5. Check for common slide preparation errors. See “Slide Preparation Checklists” on page 12.

Image Quality
QC

1. Review the scanned slide image in Aperio ImageScope.
2. Check for common slide preparation issues. See “Slide Preparation Checklists” on page 12.
3. Scan the slide again. When the scan is finished, do not remove the rack from the scanner.
4. Review the newly scanned slide image in Aperio ImageScope.
5. If the issue persists, display the slide in Slide View, and tap Scan Entire Slide. (To scan the full slide area
for a rack of slides, see page 22.)
6. Check if other slides are in focus.
7. If all slides are out of focus, clean the objective. (See the “Maintenance” chapter in the scanner user guide.)34 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Error Message

What to do

Image Transfer
Error - Pending
Retry

Error message appears on some slides:

Error message appears on all slides:

The system often resolves the issue without
intervention.

The Lab Administrator should:

1. If all slides in the rack are finished scanning,
remove the rack.

1. Check connectivity from the scanner to the DICOM
server, and the DICOM server to your site’s image
storage location.

2. Check for the image in eSlide Manager.

2. Check if your site’s image storage location is full.

3. If necessary, rescan the slides that are missing
from eSlide Manager.

3. If the issue persists, consult with your organization’s
IT professionals prior to calling Leica Biosystems
Technical Services.

35 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Error Message

What to do

AbortedError message appears on some slides:

Error message appears on all slides:

1. Check for damage or common slide preparation
issues. (See page 12.)

1. Remove any completed slide racks from the carousel.

2. Clean the slide.

2. Open the cover and access the interior. (See page26.)

3. Insert the slide into a different rack and scan it
again.

3. If there is a slide on the stage, remove it. (See page28, step 2.)
4. Close the scanner cover.
5. Shut down the scanner (see page 24.)
6. Turn the scanner back on using the On/Off switch.
7. Allow the scanner to scan any remaining racks.

36 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Error Code 1007
Internal storage full. Cannot send images to DICOM converter
The Lab Administrator should perform these steps.
1. Ensure the LAN cables are connected at the scanner LAN port and at the SAM server.
2. Run network diagnostics.
3. Ensure the DICOM server is running. Restart the DICOM server if necessary.
4. If issue persists, contact Leica Biosystems Technical Services.

37 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Error Code 2000
Slide handling error at slide stage, rack, or pusher
1. Open the scanner cover to access the interior. See page 26.
2. Take photos of the obstruction for Leica Biosystems Technical Services.
3. Check if there is a slide on the stage orpartially on the stage.
4. Carefully remove the slide from thestage without touching the surrounding
components.

5. Check for obstruction near the slide stage, rack, and pusher.
6. If possible, carefully remove the slide that is causing the obstruction, and continue tostep 8.
7. If you cannot remove the obstruction or if the issue persists, call Leica Biosystems
Technical Services.

38 | Troubleshooting

CAUTION: Restarting thescanner with a slide on thestage may damage the slide.

Aperio GT 450 Quick Reference Guide, Revision C

WARNING: Do not attempt to recover broken slides. Call Leica Biosystems Technical Services.
AVERTISSEMENT: Ne pas tenter de récupérer les lames brisées. Appeler les Services Techniques de Leica Biosystems.
8. Check the slide for preparation issues, such as over-hanging coverslips and label issues.
a. If there are no slide preparation issues, re-insert the slide in an available rack for scanning.
b. If there are slide preparation issues, correct the slide issues before scanning.
9. If the pusher is extended, return the pusher to the safe position as described in “Return Pusher to Safe Position” on page 27.
10. Close the scanner cover. (See page 26.)
11. Tap Restart Scanner.

39 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Error Code 2001
Slide handling error at rack gripper, lift, or carousel
1. Open the scanner cover to access the interior. See page 26.
2. Take photos of the obstruction for Leica Biosystems Technical Services.
3. Check if there is a slide on the stage orpartially on the stage.
4. Carefully remove the slide from thestage without touching the surrounding
components.

5. Check for an obstruction near the rack gripper, lift, and carousel.
6. Close the scanner cover. See page 26.
7. Call Leica Biosystems Technical Services.

40 | Troubleshooting

CAUTION: Restarting thescanner with a slide on thestage may damage the slide.

Aperio GT 450 Quick Reference Guide, Revision C

Error Code 9000
Scanner cover is open.
1. Slide the cover to the closed position, making sure the cover aligns with rear latches and clicks closed:

41 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Broken Slides Inside the Scanner
WARNING: Do not attempt to recover broken slides. Call Leica Biosystems Technical Services.
AVERTISSEMENT: Ne pas tenter de récupérer les lames brisées. Appeler les Services Techniques deLeica Biosystems.
Take photos of the location of the damage. Leica Biosystems Technical Services may request the photos when providing assistance.

42 | TroubleshootingAperio GT 450 Quick Reference Guide, Revision C

Index
A

E

load slides 13

Auto-Image QC 10

error codes and resolutions 29
error, rack 17

MBbarcode error 33
barcodes 12
broken slides in scanner 42
buttons 11

Ccannot find tissue 33
cannot macro focus 34
carousel 9
close cover 26
continuous loading 10
cover, open and close 26
coverslips 12

Home screen 7

macro focus error 34
macro image 20
magnification 10
maintenancechecklists 23
schedule 23

INF
firmware version, locate 25

Hicon glossary 11
image quality 10
insert slides 13

no barcode 33
no macro focus 34

Lopen cover 26

labels, slide 12
legend, rack status 17
light curtain, safety 9OP
pinch point, carousel 9

Index | 43

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preparation, slide 12priority scan 16
pusher, position 27

Q
queue, scan 20

Rrackerror 17load in carousel 14
load slides in 13
order 20
unload 15
view 19
warnings 21
remove rack 15
restart scanner
after error 28
daily maintenance 24
rotate 15
rotation, carousel 9

44 | Index

S
scan entire slide
single slide 20
whole rack 22
scan error 17
scan magnification 10
scanner
restart 24
shutdown 24
scanner overview 6
scan settings 10
scan slides 13
schedule, maintenance 23
serial number, locate 25
shutdown scanner 24
slidelabels 12
preparation 12
slide handling error 38, 40
slides broken in scanner 42
slide status 19

slide view 20
software version, locate 25
statistics 18
statuslegend 17
rack 17
scan 17slide 19
status lights 10

Ttilted slide 33
touchscreen interface 7
troubleshooting 25

Uunload rack 15

V
VPU, open and close 26

Aperio GT 450 Quick Reference Guide, Revision CWwarnings, rack 21
workflow, scan 8

Index | 45

Aperio GT 450 Quick Reference Guide, Revision C

46 | Index

LeicaBiosystems.com/Aperio

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All Rights Reserved. LEICA and the Leica logo are registered trademarks of Leica Microsystems IR GmbH.
MAN-0392, Revision C- 10/2019