DATA SHEET
20221028

Anti-PLC
(phospholipase C1)
Product Information:
1) Catalog #:Item Name:
Size :
Species :Product Code :RRID :
2) Catalog #:Item Name:
Size :
Species :Product Code :RRID :
3) Catalog #:Item Name:
Size :
Species :Product Code :RRID :MSFR104910
PLCβ1 pAb (Go) 20ug
20μg (affinity-purified with antigen polypeptide)
Goat
PLCb1-Go-Af1000
AB_2571827
MSFR104920
PLCβ1 pAb (Go) 50ug
50μg (affinity-purified with antigen polypeptide)
Goat
PLCb1-Go-Af1000
AB_2571827
MSFR104930
PLCβ1 pAb (GP) 10ug
10μg (10 g only)
Guinea pig
PLCb1-GP-Af508
AB_2571828

Formulation : Liquid ; 200 g/ml in PBS with 0.05% NaN3.
(affinity-purified with antigen polypeptide)
Storage : Store at cool temp (2-10˚C).
The antibody can be stored at 2-10˚C.

The antibody can be also aliquoted and stored at -80˚C

for long-term storage. Avoid repeated freeze-thawing. Non-hazardous. No MSDS required.
Species : guinea pig / goat,polyclonal

Antigen : mouse PLC1, 36-87 aa(TIVTPTILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAEAPKDPKLR, NM_019677)

ご注意：本商品には 0.1 % 未満のアジ化ナトリウムが入っています。
大量の水で洗い流してください。

Distributor : NITTOBO MEDICAL CO., LTD
TEL : +81-(0)3-4582-5451
email : nmd-tokuhan@nittobogrp.com

誤って目や口に入ったり、皮膚に付着した場合はManufacture : FRONTIER INSTITUTE CO., LTD

DATA SHEET
20221028

Specificity : mouse (others not tested)
Immunoblot detects a major band at 150 kDa and a minor band at 145kDa. The specificity wasverified by blank immunostaining in PLC1-KO brains. See the below reference for
immunoblot and immunohistochemistry.

Applications : In general, affinity-purified antibody is used at around 1 microgram/ml for
immunoblot and immunohistochemistry. The most appropriate concentration should bedetermined by users, because it depends on contents in given cells, tissues and organs. In use ofcryosections and microslicer sections, pretreatment of dipping sections in 1:3 methanol, 3:1
methanol, and pure methanol for 3 min each will greatly enhance immunohistochemical signals
for PLC.
Research Use : For research use only, not for use in diagnostic procedures.
Remarks : Guinea pig antibody yields slightly higher signals than goat one, but its stock issmall and limited.
Reference : Fukaya M, Uchigashima M, Nomura S, Hasegawa Y, Kikuchi H, Watanabe M:
Predominant expression of phospholipase C1 in telencephalic principal neurons and cerebellarinterneurons, and its close allocation with related signaling molecules in somatodendritic
neuronal elements. Eur J Neurosci, 28:1744-1759, 2008.
2) Hozumi Y, Fukaya M, Adachi N, Saito N, Otani K, Kondo H, Watanabe M, Goto K: Diacylglycerol kinasebeta accumulates on the perisynaptic site of medium spiny neurons in the striatum. Eur J Neurosci,
28:2409-2422, 2008.
3) Tanimura A, Yamazaki M, Hashimotodani Y, Uchigashima M, Kawata S, Abe M, Kita Y,
Hashimoto K, Shimizu T, Watanabe M, Sakimura K, Kano M: The endocannabinoid
2-arachidonoylglycerol produced by diacylglycerol lipase  mediates retrograde suppression ofsynaptic transmission. Neuron 65:320-32, 2010.

ご注意：本商品には 0.1 % 未満のアジ化ナトリウムが入っています。
大量の水で洗い流してください。

Distributor : NITTOBO MEDICAL CO., LTD
TEL : +81-(0)3-4582-5451
email : nmd-tokuhan@nittobogrp.com

誤って目や口に入ったり、皮膚に付着した場合はManufacture : FRONTIER INSTITUTE CO., LTD