Capillary electrophoresis kits
Kits to meet the challenges of today with the flexibility for tomorrow

The Power of PrecisionQuantify size and purity, either reduced or non-reduced
P/N 390953 SDS-MW Analysis Kit /P/N A10663 IgG Purity /Heterogeneity Assay
These kits were designed for the sizing and purity determination of proteins using a replaceable, gel matrix sieve. The gel is formulated to provide a sieve ranging from 10 to 220 kDa.
The preparation typically involves heating a specified concentration of sample in the presence of an alkylating or reducing agent and SDS. The analyte is then separated by size using
high-resolution capillary gel electrophoresis. This assay can detect impurities as low as 0.1% with UV detection or 0.01% with LIF detection.

P/N 390953 SDS-MW Analysis Kit

Reduced IgG

Reduced IgG

P/N 390953 kit includes:

Non-R
0.007 –

0.0065 –0.0060 –• Bare-fused Silica Capillary (3)0.006 –

0.0055 –0.005 –

0.0050 –• SDS Gel Separation Buffer

0.0045 –0.0040 –AUAU• SDS Sample Buffer

0.0035 –• Protein Sizing Standard

0.0030 –• Internal Standard, 10 kDa protein

0.0020 –0.003 –

0.0025 –• Acidic Wash Solution, 0.1 N HCl

0.0015 –• Basic Wash Solution, 0.1 N NaOH

0.0005 –0.002 –
0.001 –

0.0010 –0.000 –
8.0

8.5

9.0

9.5

10.0 10.5

11.0

11.5

Minutes12.0 12.5 13.0 13.5 14.08910

Non-Reduced IgG

Reduced IgG

P/N A10663 IgG Purity/Heterogeneity Assay

0.004 –

Non-Reduced IgG

0.007 –
0.0065 –P/N A10663 kit includes:
0.0060 –0.006 –

0.0055 ––• SDS Gel 0.0040
Separation
Buffer

0.004 –

AU0.005 –AU0.0050 –• Bare-fused
Silica Capillary (3)0.0045 –0.0035 –• SDS Sample
0.0030 –Buffer

Aggregates

0.003 –

0.0025 –• IgG Standard
0.0020 –0.002 ––• Internal 0.0015
Standard,
10 kDa protein

0.001 –

0.0010 –• Acidic Wash
0.0005 –Solution, 0.1 N HCl

0.000 –

8.0
8.5
9.0
10.0 10.5 11.0 11.5 12.0 12.5 13.0 13.5 14.0
• Basic Wash Solution,
0.1 9.5
N NaOH
Minutes

89101112131415

Minutes1617181920

Reduced and non-reduced NIST mAb ran reduced in <15 minutes andnon-reduced in <18 minutes.

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111121High-resolution, broad range RNA integrity and quality analysis
P/N C48231 RNA 9000 Purity & Integrity kit
Say goodbye to less than desirable analytical resolution, poor method transferability and method complexity when analyzing RNA products. Now you can easily
analyze a diverse array of RNA species, from 50-9,000 bases, and achieve high-resolution data down to double digit base differences – all with the SCIEX RNA 9000
Purity & Integrity kit. Analysis from early development to QC can easily be conducted with transferability and validation completed on the BioPhase 8800 systemand the PA 800 Plus.

Rel. Fluorescence Units (RFU)

P/N C48231 RNA Purity & Integrity kit

3-2-1-0|

12.5|

15|

17.5

|

20Time (min)Kit includes:

SCIEX RNA 9000 Purity & Integrity kit used to analyze mRNA purity frompreviously LNP encapsulated mRNA samples from SINTEF Industry (Norway).²• Nucleic Acid Extended Range Gel (2)• SYBR™ Green II RNA Gel Stain1 (500x)• Acid Wash/Regenerating Solution (0.1M HCl)
• CE Grade Water (2)• LIF Performance Test Mix• ssRNA Ladder (0.05 kB to 9 kB)
1

SYBR™ is a trademark of the Life Technologies Corporation. SYBR™ Green II RNA Gel Stain is not available for resale.

For more information, visit: sciex.com/products/consumables² LNP encapsulated mRNA, mRNA, and empty LNP samples provided by Jérémie Parot, PhD, SINTEF Industry (Norway).

2

Highly sensitive and effective DNA and plasmid analysis
P/N 477480 ssDNA 100R Kit / P/N 477410 dsDNA 1000 Kit
Use either UV or enhanced fluorescence detection with laser-induced florescence (LIF) for single base resolution of DNA and plasmid products. SCIEX offers bothssDNA analysis of individual bases or dsDNA analysis of thousands of base pairs. These kits are ideal for plasmid and oligonucleotide purity analysis as well as DNAintegrity. These kits include a coated capillary, replaceable gel, test mix, and internal standard guaranteed to perform at least 100 runs.
P/N 477480 ssDNA 100R Kit
P/N 477480 kit includes:• DNA Capillary (2)• ssDNA 100R Gel• ssDNA 100 Test Mix• Tris-Borate Buffer
1.5 kb• 7 M Urea

• DNA Capillary (2)• dsDNA 1000 Gel (3)• dsDNA 1000 Text Mix (2)• Orange G Reference Marker

For more information, visit: sciex.com/products/consumables

10 kb

8 kb
15 kb

7 kb

6 kb

5 kb

3 kb
4 kb

1.353 kb
2 kb

0.603 kb

0.65 kb

0.5 kb

0.4 kb

0.3 kb0.310 kb

16

0.271 kb

0.2 kb150.281 kb

5–0.234 kb

P/N 477410 kit includes:

0.194 kb

10 –0.118 kb

P/N 477410 dsDNA 1000 Kit

0.072 kb

15 –0.1 kb

20 –Injection MarkerRFU25 –2.685 kb

0.85 kb

30 –0.872 kb
1.078 kb 1 kb

• pd(A) 40-60 Test Mix

0–121314

17

18

19Minutes

2021222324

25

Separation of virus and plasmid DNA utilizing SCIEX dsDNA 1000 kit(Sample peaks are in green with DNA ladder peaks in black font.)

3

Charge heterogeneity analysis in as little as 5 Minutes
P/N C44790 CZE Rapid Charge Variant Analysis Kit
Charge variant analysis has never been easier. In under 5 minutes of separation time, the CZE (Capillary Zone Electrophoresis) Rapid Charge Variant Analysis
Kit can resolve a native state molecule’s charge variants with little-to-no method development and provide higher resolution than icIEF or ion exchange
chromatography. This kit provides all of the necessary reagents to analyze a molecule’s charge variants based on its mobility. This method provides a fast,
powerful separation used to quantify charge variants with a buffer that serves as both a separation matrix and dynamic coating for a bare fused silica capillary.
In addition, no sample dilution buffer is required; simply dilute the sample in water and you are ready for your separation.

0.060

0.055

AU

0.045

0.035

0.015

Kit includes:• CZE Rapid Charge Variant Separation Buffer

0.000
2.0

2.6

3.2

3.8

4.4

5.0

Minutes• Acid Wash/Regenerating Solution• CE Grade Water (H2O)• Protein Test Mix

For more information, visit: sciex.com/products/consumables

Trastuzumab charge heterogeneity profile separated utilizing the CZE RapidCharge Variant Analysis Kit.

4Two times faster than HILIC with less sample prep
P/N B94499 Fast Glycan Labeling and Analysis Kit
This kit was designed to rapidly label and separate released N-Glycans from glycoproteins. This method utilizes 8-aminopyrene-1,3,6-trisulfonic acid (APTS) followed by the effective removal of
the excess dye using a magnetic bead based cleanup. The labeled glycans are then separated by high resolution capillary electrophoresis with laser induced fluorescence detection using
the HR-NCHO separation matrix. Immediate glycan identification is determined based on their normalized electrophoretic mobility referenced against pre-determined Glucose Units (GU)values using appropriate bracketing standards.

110 –

FA2

110 –

100 –

2.0

2.2

2.4

2.6

2.8

3.0

3.2

A2G2S1

50 –
40 –

20 –

3.4

Migration Time (min)

3.6

3.8

4.0

4.2

10 –
0–

DP230 –

2.00

2.25

Adalimumab separated and identified 10 glycan species in <5 minutes

110 –

FA2

110 –

2.0

2.2

2.4

2.6

2.8

3.0

3.2

A2G2S1

3.4

Migration Time (min)

3.6

3.8

4.0

4.2

0–

2.00

2.25

A2G1S1FA2[6]G1

DP15

10 –

FA320 –

FA2[3]G1
A2G2
FA2G230 –M6
A2[3]G1
FA2B

40 –FA2[3]G1S1

FA2G2S250 –A2 M5• Pre-Assembled Cartridge

0–60 –A2G2S2• Magnetic Separator• L5 Mix

10 –

70 –

FA2[6]G1S1• D4 mix

20 –80 –DP3

• HR-NCHO Separation Gel

30 –90 –DP2• D3 Mix

Relative Fluorescence Unit

• BST (Bracketing Standard Mixture)

40 –

DP15

• D2 Mix

50 –

FA2G2

• IST (Internal Standard)

60 –A3 FA1G1
FA3 FA2(6)G1
FA2(3)G1• D1 Mix

70 –

A2M5

• GU Ladder

80 –

FA1

• M1 Mix

100 –

90 –

DP2

Kit includes:

Relative Fluorescence Unit

100 –

2.50

2.75

3.00

3.25

3.50

Migration Time (min)

3.75

4.00

Enbrel® separated and identified 10 glycan species in <5 minutes• L6 Mix

For more information, visit: sciex.com/products/consumables

A2 M5

A2G1S1

60 –

DP30–

FA1

10 –

A2
M520 –

DP15

30 –

FA2G2

40 –

A3 FA1G1
FA3 FA2(6)G1FA2(3)G1

50 –

70 –
FA2G2S2

60 –

80 –

FA2[3]G1S170 –90 –A2G2S280 –FA2[6]G1S1

Relative Fluorescence Unit

90 –DP2

Relative Fluorescence Unit

100 –52.50

2.75

MigrHighest pI resolution available
P/N A80976 Advanced cIEF Starter Kit
Capillary Isoelectric Focusing (cIEF) is a powerful technique that allows quantitative, experimental analysis of a protein’s isoelectric focusing point (pI) andcharge variants. In cIEF, a mixture of sample and ampholyte is introduced into a capillary and subjected to a high voltage, creating a pH gradient throughwhich analytes migrate to their respective pI. The PA 800 Plus system automates the process to successfully achieve high precision and quantitative separations.
Use of optimized universal methods and synthetic pI markers attains the highest levels of precision (down to 0.03 pI units) in pI calculation and direct isoform
quantification with online UV detection.
0.08 –
0.07 –

IgG
0.06 –

AU

0.05 –
0.04 –

5.5

0.03 –

7.0

6.7

0.02 –
0.01 –
0.00 –

15

Kit includes:• Neutral Capillary• cIEF Gel• pI Peptide Marker Kit (pI Marker 4.1, 5.5, 7.0, 9.5, and 10.0)• Sample Loading Solution (SLS)• CE Grade Water

16171819202122

Minutes

23242526272829

pl exppl exppl expPeakMean

Std Dev%RSD

pl 7.0

6.957

0.008

0.115

pl 6.7

6.741

0.007

0.103

IgG a

6.473

0.007

0.114

IgG b

6.381

0.007

0.115

IgG c

6.308

0.007

0.117

IgG d

6.232

0.009

0.152

pl 5.5

5.492

0.009

0.158

30

CIEX cIEF separation method delivering high accuracy of pI markers coupledwith extremely robust peak pI identifications of mouse IgG1k.

For more information, visit: sciex.com/products/consumables

6

Develop your ideal method
P/N 477445 Protein Methods Development Kit
Our Protein Method Development Kit is a great place to start your assay development. It is designed as a starting point for the development of a capillary zone
electrophoresis analysis of complex molecules. The Protein Method Development Kit contains a variety of capillaries, buffers, standards, and markers to allow
you to optimize a separation method for a broad spectrum of proteins by utilizing their mass/charge characteristics. This kit enables a user to develop acustomized method that is ideal for their molecule.
P/N 477445 Protein Methods Development Kit

0.030 –
0.025 –

Histamine IgG

AU0.020 –0.015 –0.010 –0.005 –0.000 –

Kit includes:• Neutral Capillary• Orange G Reference Marker• Histamine Reference Marker

024

68

10

12

Minutes141618202224IgG mobilized against the Histamine reference marker with separationin <6 minutes.

• Citrate Buffer, pH 3
• Citrate/MES Buffer, pH 6
• Tricine Buffer, pH 8
• Protein Test Mix

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7Supplies and resources
Items can be ordered at www.store.sciex.com or from your local SCIEX representative.
Description
Pre-Assembled Cartridge
Cartridge Assembly Kit
Universal Vials
Universal Vial Caps
200 µL Microvials (Pkg of 100)nanoVials (Pkg of 100)
96 well plate (Pkg of 100)
Bare-Fused Silica Capillary, 67 cm total x 50 µm ID (3)Bare-Fused Silica Capillary, 37 cm total x 20 µm ID (3)Bare-Fused Silica Capillary, 67 cm total x 75 µm ID (3)Part No.
A55625144738A62251
A622501447095043467609844338451338475338454Bare-Fused Silica Capillary, 111 cm total x 75 µm ID (3)360800Bare-Fused Silica Capillary, 111 cm total x 50 µm ID (3)360801DNA Capillary477477

Neutral Capillary477441NCHO Capillary477601

Coolant tubing, 100 cm

144717

For more information, visit: www.store.sciex.com

8

Notes

For more information, visit: sciex.com/products/consumables

10NotesFor more information, visit: sciex.com/products/consumables

11

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The Power of Precision