• Increased coverage fromlimited samples• High-resolution separationof polar analytes andcritical PTMs
• In-depth characterizationof proteoforms andprotein complexes

The Perfect Accessory for Your Mass Spectrometer
CESI 8000 Plus High Performance Separations ESI Module and Adapter Kit
CESI is a mass spectrometer accessory for the analysis of biological compounds in every analytical laboratory:• Consumes minute sample amounts (nL volumes) with extraordinary sensitivity• Excels at charged & polar metabolites, peptides, and more
• Enables high-resolution separation of proteins and protein-complexes• Eliminates ion suppression so you can see what you are missing• Robust and easy-to-use thanks to zero-dead-volume design

CESI-MS “showed better separation efficiency andresolution with 100-fold less sample consumption
compared to an LC-based intact protein separation.”
J. Prot. Res. 2014 from the John Yates III research group,
The Scripps Research Institute, La Jolla, CA.

CESI-MS “shows a separation efficiency for highmolecular-mass compounds like larger peptides
and proteins that is remarkably higher than thatseen with any LC method”
Molecular and Cellular Proteomics 2013 from theHerbert Lindner research group, Innsbruck, Austria

Connecting the CESI 8000 Plus to your mass spectrometer is aseasy as plug and spray. Attach the OptiMS Adapter to your massspectrometer and click the CESI cartridge tip into place.

Total Ion Electropherogram

Intact MAb Separation of IgG1 by CESI-MS
Reconstructed Intensity

Ion Intensity

6E+83E+83000
2500
2000
1500
1000
500
0113

100156161

134■ Phosphorylated Peptides (n=57)

85■ Acetylated Peptides (n=688)
32

910131197646432111020

130721405060

30001470e5Deamidations

146389.09

146569.16

2000
1500

0Deamidations
146701.58

146156.71

Deamidations
and oxidation

146862.70
146981.51

1460e51465e51470e5Mass. Da

2000

146573.04

1500146536.36

1000
500

146387.58

2500

146535.62

1000
146719.51500
0146189.93

1460e5146948.99

1465e51470e51475e5Mass. Da

Different glycosylation and modification profiles

Kindly provided by Prof. Herbert Lindner, Innsbruck Medical University, Austria.

For deep characterization of proteoforms, glycoforms, chargestates, and PTMs, CESI is the only front-end technology thatenables liquid-phase separation of proteins, metal binding,and protein complexes. CESI-MS is an invaluable tool todrive biopharmaceutical development as well as biochemicalmechanistic studies.

PSA Profiling Under Native Conditions

14 cells / µL, 4 cells injectedm/z

1465e5

2500

Time (Min.)

CESI-MS excels at various PTMs revealing importantbiological functional variations.

1460e5Mass. Da

■ Non-modified Peptides (n=688)

Reconstructed Intensity

Peptides Identified0

pH 7.6: base ion electropherogram (A); density ion map of a separation
highlighting the CE migration patterns of proteoforms (B); mass spectra (C);and corresponding deconvoluted spectra (D).

101022J000006__01_1683.d: BPC 49.0-3001.0 +All MS

1500
1000500

Intens.
x105

102030405060 Time [min]102030405060 Time [min]

0.8
0.6
0.4
0.20.0CESI enables MS signal from minute amounts of sample.

CESI-MS-EMR reveals 236 proteoforms from 2.9 ng of PSA undernative conditions.

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© 2016 AB Sciex. For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners.
AB SCIEX™ is being used under license.
RUO-MKT-07-3980-A 05/ 2016

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