京都大学教育研究振興財団助成事業
成 果 報 告 書
平成３０ 年

11月 20日

公益財団法人京都大学教育研究振興財団会 長 藤
洋 作 様
所属部局・研究科

大学院農学研究科・域環境科学専攻

職 名・学 年

博士課程3年氏

Nguyen Xuan Dong

名助 成 の 種 類

平成 ３０年度 ・研 究 集 会 名The 9th International Conference on Mushroom Biology andMushroom Products

発 表 形 式□ 招 待

発 表 題 目

Functional Analaysis of promoters using Transfection, Random
Integration and Gene Knock-in in Mushroom-forming fungi

開 催 場 所

Holiday Inn Shanghai Hongqiao（中国・上海）

渡 航 期 間平成 ３０年

成 果 の 概 要・国際研究集会発表助成■ 口 頭

１１月１１日・ □ ポスター ・ □ その他（～）

平成 ３０年 １１月 １６日

タイトルは「成果の概要／報告者名」として、A４版２０００字程度・和文で作成し、添付して下さい。 「成果の概要」以外に添付する資料 ■ 無□ 有()
交付を受けた助成金額

150,000 円

使 用 し た 助 成 金 額

150,000 円

返納すべき助成金額0円
飛行機代（関西空港への交通費も含む）： 58,270円

会 計 報 告宿泊費（5泊）: 40,000円
助 成 金 の 使 途 内 訳

VISA取得費用（大阪への交通費も含む）： 12,400円

大会参加登録費： 33,000円
上海での交通費・食費を含む滞在費： 12,000円
合計155,670円に助成金を充当（今回の助成に対する感想、今後の助成に望むこと等お書き下さい。助成事業の参考にさせていただきます。）

当財団の助成について/ Nguyen Xuan Dong
Program: the 9th International Conference on Mushroom Biology and Mushroom
Products (9th ICMBMP)
Venue:

Holiday Inn Shanghai Hongqiao, Shanghai, China

Date:

November 12- November 15, 2018
Title of my presentation

Functional Analysis of Promoters Uusing Transfection, Random Integration andGene Knock-in in Mushroom-Forming Fungi
The International Conference on Mushroom Biology and Mushroom Products(ICMBMP) founded in 1993 by World Society for Mushroom Biology and Mushroom
Products. At the beginning, this conference only focus on scientific research of edible
and medicinal mushrooms, and the development and marketing of mushroom products.
But now day, WSMBMP conference cover various aspects of mushroom science andthe mushroom industry, such as mushroom taxonomy, genetics and molecular biology,
but also cultivation technique, pests and diseases and quality control.
This year, the 9th ICMBMP hold in Shanghai and Zhangzhou, China from November
12-19, 2018. The academic conference took place in Shanghai from November 12-15,
and the mushroom product exhibition was hold together with the 12th Chinese
Mushroom Days from November 16-19 in Zhangzhou, Fujian Province. The 9thICMBMP incorporated the latest advances relating to all aspects of mushroom biology
and mushroom products in the form of keynote lectures, oral presentations and poster
displays. Panel topics include 1) Diversity and Taxonomy; 2) Omics andBioinformatics; 3) Genetics and Breeding; 4) Physiology and Development; 5)
Mycosourced Molecules and their Nutritional and Medicinal Properties; 6) Mushroom
Cultivation, Substrates, Factory Production and Equipment Innovations; 7) Pest andDisease Management; 8) Product Quality and Safety Controls; 9) Mushroom
Economics and Cultural Impacts. In this conference, the latest participation count stands
at about 570 participants from 30 countries over the world. And 108 oral presentationand 52 posters were presented at that time.
I made my oral presentation om 14 November, 2018 and a title is: ‘Functional analysis
of promoters using transfection, random integration and gene knock-in in mushroomforming fungi’. The presentation is about investigation molecular mechanism
underlying transcriptional regulation in Agaricomycetes. We know that transcription
initiation and its regulation play the crucial role in the activation and suppression ofgene expression, and are mainly controlled by DNA elements including the coreelement and others cis-acting elements in the gene promoter. Even though many studies
have been conducted to identify and characterize cis-acting element in eukaryote, andsome papers have reported existence of putative cis-acting elements in mushroom. Theirfunction is still vague in mushroom due to lack of an appropriate system to analyse
promoter. Therefore, we try to apply three promoter assay systems; transfection,
random integration and gene knock-in to elucidate cis-acting elements in mushroom
promoters. From our results, new core promoter element was found out in ß1-tubulinpromoter of Agaricomycetes, and location of Avicel-responsive element and Manganeseresponsive element are continuously finding in Coprinopsis cinerea Cel6A and Pleurotus
ostreatus mnp3 promoter, respectively. Our result also suggested the advantages anddisadvantages of three different promoter assays: 1). Transfection is very powerful tool toidentify concrete sequence of cis elements, but it requires to establish a suitable selection
system in each fungus. 2) Random integration can be rather widely applied to species that canbe genetically transformed but we have to be careful about different positions and copy
numbers of the reporter on the chromosome. 3) Knock-in seems most appropriate method toconfirm the function of a putative cis element, but it requires a special host strain deficient in
Non-Homologous End Joining system. And it is to be considered if the integrated site has thesame regulatory property as the original position of the promoter of interest on the chromosome.
After finished my presentation, I got many useful comments to improve my study, andinterested questions from audiences.

During the conference, I attended workshop ‘Genetic diversity and molecular breedingin cultivated mushrooms’ and varieties of presentation and key note speech, which
inspired me with new ideas and knowledge. I also had a very good discussion andinteraction with many experts of my field. All these learning is very useful to improvemy study in coming days.
Finally, I would like to thank ““ ”.

” again for your grant of

Figure 1: ‘Genetic diversity and molecular breeding in cultivated mushrooms’
workshopFigure 2: My oral presentation in 9ICMBMP