SCIEX 6500+ series mass spectrometers

Revolutionary sensitivity, speedand performance
Enhance your capabilities

The Power of Precision

Enhance your capabilities with SCIEX 6500+ series
mass spectrometers
Your most challenging analytes can’t hide. The 6500+ series offers revolutionary sensitivity, speed and performancedelivered through technology enhancements designed to see it all.

A solution without compromise
Low mass or high mass, positive or negative polarity—detect and identify analytes across a wide scope of chemistries
at once. The SCIEX 6500+ series with IonDrive technology enhances performance across key applications:•Small and large molecules in bioanalysis•

Priority environmental contaminants•

Comprehensive food residue monitoring•

Biomarker verification•

Forensic drugs in complex matrices

All backed by the integrity and quality of the SCIEX brand.

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SCIEX 6500+ series IonDrive technology
combined with novel detection system
Visionary sensitivity

Intelligent by design

The SCIEX 6500+ series with IonDrive technology,

IonDrive technology is built into the system, from theimproved detection system, and elevated SelexION+ionization source, through the ion-focusing region,differential mobility spectrometry, merges outstanding

all the way to the high energy detector, increasing

sensitivity with renowned performance to give you thethe number of ions produced, the efficiency of ion

lowest limits of quantification (LLOQ) for compounds oftransmission and the way ions are detected. The

many classes, even in complex matrices, for robust andaddition of SelexION+ with jet injector technology

reliable results.

adds an additional boost in performance when added
selectivity is required.
Analysis without compromise
The enhanced robustness and up to 6 orders ofdynamic range significantly increases the breadthof applications possible with the 6500+. From small
molecules to large molecules, and across polarities,
the 6500+ series delivers sensitive quantitative results
without compromise.
SelexION+ is proven to deliver the added selectivity
you need without compromising LLOQs.
QTRAP for your most challenging questions
Driven by proven Linear Accelerator trap technology,
the QTRAP 6500+ system enables enhanced MS/MS
workflows even with UHPLC approaches. And, forcomplex samples, the selectivity of the MRM3 workflow
significantly enhances data quality while reducing theneed for added sample preparation.

3IonDrive technology—pushing the sensitivity barrier
SCIEX has been designing MS instruments for the past 50 years. Each platform introduced is the culmination of years of research and development in improving sensitivity,
accelerating throughput and enhancing data quality. IonDrive technology with the enhanced high energy detector and SelexION+ technology built into the 6500+ series
LC-MS/MS systems pushes the boundary even further.
IonDrive technology simultaneously targets 3 critical areas of enhancements in the 6500+ series, ruggedly driving best-in-class performance improvements and unrivaledsensitivity in 3 key components of the system:•The production of more ions with the optimally designed IonDrive Turbo V ion source•The capture and transmission of more ions with the unique IonDrive QJet ion guide•The detection of more ions with the enhanced IonDrive high energy detector+

IonDrive technology—driving performance

IonDrive Turbo V ion source

4IonDrive QJet ion guide

IonDrive high energy detector+

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Produce more ions, capture more ions, detect more ions
Sensitivity and ruggednessIonDrive Turbo V ion source
Get more sensitivity without sacrificing robustness andruggedness through enhanced gas flow and optimized heater
configuration compounded with the legacy Turbo V design.

The IonDrive Turbo V ion source maintains
the quick-change APCI and TurbolonSprayprobe as well as adaptability to the low
dispersion electrodes for use with microflow
UHPLC methods.

From 5 µL/min to 3 mL/min,
the IonDrive Turbo V ion
source is the perfect matchfor narrow bore standard
The enhanced gas flow dynamicsand optimized heater configurationsdeliver improved reliability,reproducibility and robustness.

6bore and UHPLC flow rates.

The tool to take on your most challenging assays
The 6500+ system is designed to produce more ions, transmit moreions and detect more ions. The optional QTRAP technology offers
the added value of both simultaneous qualitative screening andreliable quantification in a single injection.

Increase confidence
QTRAP technology
Boost selectivity for added confidence in your most challenging assays with enhanced
mass spectrometry scanning modes that deliver MRM and so much more. Take advantage
of 20,000 Da/s scan speed and full scan linear ion trap sensitivity improved by 100x over
the SCIEX Triple Quad system for greater confidence in quantitative workflows.

Detection without bias
IonDrive high energy detector
Get consistent and reliable quantitative results
for all ions, regardless of polarity, chemistry andconcentration, all in a single injection.

More MRMs per injection
Curved LINAC collision cell

Enhanced sensitivity
IonDrive QJet ion guide
Better collisional focusing means more ionstransmitted, enhancing analyte sensitivity. The
optimized design better contains ions, operates athigh pressure and lets the turbopump run cooler
and in its ideal operating range for peak performance.

Achieve shorter MRM cycles and higher scan speeds toanalyze for hundreds of compounds in a single injection
for improved throughput. With true collision-induced
fragmentation, this collision cell will generate reliable,
information-rich, library-searchable MS/MS spectra
time after time.

7The SCIEX Triple Quad 6500+ system

Ultra-sensitive and robust for definitive quant
The Triple Quad 6500+ system delivers ultra-low limits of quantification with significant enhancements in everything from ion production, to ion focusing andtransmission, and through to detection. The results speak for themselves.

8•Best-in-class MRM sensitivity•

Up to 6 orders of linear dynamic range for excellent quantification•

2000 m/z upper mass limit•

Verified inter- and intra- instrument robustness with the IonDrive Turbo V ion source•

Optimized UHPLC strategies with scan speeds of up to 12,000 Da/sec, to acquire more data points per peak•

Increased throughput with polarity switching speeds of 5 ms•

Improved selectivity with SelexION+ differential mobility spectrometry

Continuous improvement in ultra-trace level quantification
Enhancing LOQs for the most challenging analytes

The IonDrive high energy detector+, delivers significant performance improvements, enabling even lower limits of quantification for challenging analytes that ionize in both positive and negativemodes, as well as improving switching between modes to quantify more compounds in each injection.

positive polarity (552 MRMs)

MRM Transition 1

MRM Transition 1

MRM Transition 1

MRM Transition 1

MRM Transition 2

MRM Transition 2

MRM Transition 2

MRM Transition 2

negative polarity (58 MRMs)

Extended compound detection in a single run
Ultra-fast polarity switching at rates as low as 5 ms combined with Scheduled MRM

Ultra-trace detection of ions in both positive andnegative ESI

Pro algorithm provides exceptional detection of 100s of compounds, regardless ofAnalytes of all shapes, sizes, and polarities are no match for the 6500+ system. Lowionization polarity, in a single injection. Acquisition speeds are fast enough to deliver
sufficient data points across chromatographic peaks for accurate quantification evenin areas with many co-eluting peaks.

level detection of perfluroalkyl acids including perfluorooctanoic acid (PFOA) (shownhere) in drinking water at levels of 1 ng/L, with CVs less than 8% and excellent
quantitative linear dynamic range is easily achievable.

9Enhance your methods with QTRAP systems
Evolved functionality for accelerated performance
See the full MS/MS picture for every MRM

MRM3—a generation of quantification without

Automated MRM3 method method-building scripts

When combined with the QTRAP technology, theinterferences

makes parameter definition effortless while also making

6500+ series delivers the MRM sensitivity you need forWhen high background or challenging co-eluting

the MRM3 workflow a fast, reproducible, and easy way toquantification, with added 100X increase in full-scaninterferences make standard MRM quantification

sensitivity over basic triple quads. The combined triple

difficult, enhanced quantitative selectivity is a mousequadrupole and linear ion trap scan functions provide

click away with MRM3. The QTRAP 6500+ system

unrivaled confidence by complementing sensitive MRM

enables MRM3 scans that are twice as fast as previous

detection with enhanced product ion and complete

generations of QTRAP technology, enabling

MS/MS profiles for unequivocal identification.

faster chromatography.

Enhanced selectivity in compoundidentification with MS/MS

MRM3 for enhanced peptide quantification

Propamocarb from Cucumber Using MRM

QTRAP technology enables collection of high-quality MS/MS spectra in
addition to MRMs to enable library comparisons for better confidence in
MRM 90 µg/kg

compound identification.

Propamocarb from Cucumber Using MS/MSThe MRM3 workflow can often provide higher specificity and therefore
better LLOQs in complex matrices over MRM workflow alone, as shown here
for the heavy labeled peptide DRVYIHP in digested human plasma.

MS/MS Fit score = 100%

10increase throughput.

Front-end solutions for your most challenging assays
Regardless of the challenges encountered in the development of your assays, you can have confidence that the 6500+ series has the tools youneed when you need them.

Double your productivity

OptiFlow Turbo V ion source

The optional DuoSpray Turbo V ion source contains

A revolution in microflow separations, the OptiFlow

the TurboIonSpray and APCI probes in one housing

Turbo V ion source supports flow ranges from 1 towith computer-controlled switching, allowing you to200 µL/min, with a simple tool-free set-up and no

optimize ionization techniques and conditions for eachmanual adjustments needed to achieve optimalcompound during an LC run. It’s ideal for fast method

spray conditions.

development as well as increasing throughput anddata quality.

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A world of options—a new dimension in selectivity
Differential ion mobility separation

The enhanced SelexION+ differential mobility spectrometry with jet injector technology has been redesigned for improvedtransmission in ion mobility detection. More ions + less noise = sensitivity delivered.
Improve data quality and enhance selectivity for challenging samples that require advanced analytical separations. The 6500+ series with SelexION+ is the ideal development suitefor any application requiring the separation of isobaric species, isolation of challenging co-eluting contaminants and reduction of high background noise.

12Added selectivity without compromise
Improved SelexION+ with jet injector technology

Add selectivity with ion mobility without sacrificing limits of quantification or dynamic range. The re-designed SelexION+ with jet injector technology improves ion transmission efficiency
for large and small ions alike.
A) Without SelexION+A) Without SelexION+

Alprazolam %CV = 3.5%

Triazole solvent
standard 0.5 ng/L

Buprenorphine %CV = 7.9%
Spiked and extracted
fruit (10 µg/kg)

B) With SelexION+

B) With SelexION+

Clenbuterol %CV = 2.9%

S/N ~ 60

Methamphetamine %CV = 2.8%
S/N ~ 390

Enhanced selectivity for small
molecule quantification

Improving peptide and large
molecule analysis

Robust quantification across 1000sof injections

The power of SelexION+ DMS is palpable when faced

Ion mobility is no longer just for the small. Somatostatin,

The 6500+ system with SelexION+ delivers reliablewith the analysis of low level compounds in challenging

a cyclic peptide with poor MS/MS fragmentation, wasperformance, around the clock. For the analysis ofmatrices. Clear reduction of matrix interferences foranalyzed in rat plasma. The utilization of SelexION+ for thecompounds in crashed plasma, peak intensities remained

Triazole analyzed in fruit samples were observed,

analysis improved the S/N for the peptide by a factor ofstable, with less than 5% CV for many compounds, acrosswith delivery of a highly sensitive peak clear of matrix

4, enabling significantly improved quantitative results

4000 injections.

interferences for more reliable quantification.

at 100 pg/mL.

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Analyst software

The validated environment designed for quantification. Proven and accepted
throughout the world.
Acquire the best data

deployed LC-MS/MS software for drug discovery

Industry-standard Analyst software for data acquisitionand development.

utilizes the intelligent Scheduled MRM Pro algorithm tomake method set-up 1000s of MRMs in a single analysissimple, with optimized parameters to ensure you get

Comprehensive compliance services•Analyst service is built into the Analyst software
platform, so it stays running even when you log
off Windows

The SCIEX professional services team provides

The unique Analyst Administrator Console
provides multi-instrument, project-wide security
from a single computer

LC-MS/MS solution. You receive a holistic approach to

Configurable audit map prevents redundant or
unnecessary event capture

workflow including your mass spectrometer, HPLC,•Analyst software is designed to be used as part of a21 CFR Part 11 compliant system with years of use•

in the field, innovation and acceptance by regulated
laboratories worldwide. Today, Analyst software
continues to deliver confidence and data integrity•Network acquisition for efficient data storage
and backup

with every report and has become the most widely

The gold standard—our customers“Analyst software security settings are highly customizable ensuring we can meet regulatory and customer
expectations for our daily data processing routines.”
Dr.C. Briscoe, Senior Director,
US Bioanalysis, PRA International

14A defined Analyst software project structure keeps all
relevant data interlinked

What makes Analyst software different?

exceptional data quality in every analysis.
The pedigree that defines regulated bioanalysis•

integrated analytical instrument qualification andsoftware validation packages to cover your complete
compliance that includes qualification, re-qualificationand software validation for your complete SCIEX system
software, reagents and test kits.

One-touch productivity

Powerful, workflow-driven software ties everything together to deliver a new
benchmark in efficiency, throughput and productivity.

SCIEX OS-MQ software
Increased quant productivity

SCIEX OS-Q software
Well-suited for qual and quant data analysis

The solution for quantifying large sample sets features

The software simplifies compound identification,

superior data processing and visualization, powerful data

quantitation and data review, so your lab can master theintegration, enhanced audit trail and helps users of different

speed, power and accuracy offered by LC-MS/MS technology.

skill levels to perform their own automated analyses.

From performing high-throughput screening MRM dataprocessing to non-targeted investigations, all the tools youneed are at your fingertips, without sacrificing time, valuable
samples or results.

ProteinPilot software
Streamlined protein ID and quantDiscoveryQuant software
Automated quant for drug-like molecules

Identify hundreds of peptide modifications and non-tryptic

Reduce tedious method development time withcleavages, distinguish isoforms, suppress false positive

automated, efficient and rapid LC-MS/MS drug

results and quantify proteins and peptides across many

candidate screening workflows.

samples simultaneously.

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SCIEX Now support network

The destination for all your support needs

Data integrity

Increase your confidence with complianceservices to help you safeguard your data,
confirm data integrity and ensure systemmodifications can be traced.

Onboarding
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Onboarding

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A holistic approach to your lab toincrease productivity and decreasesystem downtime. We help you
meet tight deadlines, improve thequality of results and ultimately
increase your profits.

Labenhancement
services

SCIEX Now

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Best-in-class content, personalized learning
paths—delivered using the latest memory
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L a b g ro w t h

Self-help resources
Our knowledge base and community help you
move your science forward and find the answers
you need from SCIEX experts as well as your peers.

Workflow support
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resources

Workflow
support

Whatever your challenge, the SCIEX support teamis here to help you achieve your scientific goals
quickly and efficiently.

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The Power of Precision