Day 1 Program Nov. 28 (Tue)Opening Remarks

13:00~13:15

Lecture Room 1 [141+142]

Symposium 1: Chicken as the Crossroads of Basic and Applied Research Fields

13:15~15:15 Lecture Room 1 [141+142]

Organizers: Yuya Okuzaki(Nagoya University), Ken-ichi Nishijima(Nagoya University)
Chairs: Yuya Okuzaki(Nagoya University), Yoshinori Kawabe(Kyushu University)
Co-hosted by Avian Bioscience Research Center, Nagoya University
Outline: Chickens has been used for the basic sciences including developmental research. Recent advances in transgenic
technology provide the opportunities to use transgenic/genome-editing chickens in new applications. These include thebioproduction of pharmaceutical proteins, the reduction of allergen from eggs, and resistance for severe poultry diseases like
influenza. In this session, several topics on such applied fields as well as new trials in chicken basic research will be discussed.

S1-01 Generation and application of transgenic chickens towards robust production system of recombinant proteins
13:15

Yoshinori Kawabe1, Ken-ichi Nishijima2, Masamichi Kamihira11Dept. Chem. Eng., Fac. Eng., Kyushu Univ., 2Grad. Schl. Bioagr. Sci., Nagoya Univ.

S1-02 Transcription Activator-Like Effector Nuclease-Mediated Deletion Safely Eliminates
13:45
the Major Egg Allergen Ovomucoid in Chickens
Ryo Ezaki1, Tetsushi Sakuma1, Daisuke Kodama2, Ryou Sasahara2, Taichi Shiraogawa2,
Kennosuke Ichikawa1, Mei Matsuzaki1, Akihiro Handa3, Takashi Yamamoto1, Hiroyuki Horiuchi1
1

Hiroshima University, Japan, 2 Kewpie Corporation, Japan, 3Tokyo Denki University, Japan

S1-03 Application for iPS cell technology to avian species
14:15

Masafumi Katayama1, Tomokazu Fukuda2
12

National Institute for Environmental Studies,
Graduate School of Science and Engineering, Iwate University

S1-04 Genome editing of poultry species for investigating disease resistance
14:45

Kennosuke Ichikawa, Lorna Taylor, Tuan Jun, Alewo Idoko-Akoh, Sudeepta Panda,
Mike J. McGrew
The Roslin Institute, University of Edinburgh

Symposium 2: Regenerative Medicine Driven by Advancing Technologies

15:30~17:30 Lecture Room 1 [141+142]

Organizers: R yuji Kato(Nagoya University), Kazunori Shimizu(Nagoya University)
Outline: Many advanced medical sciences, including regenerative medicine and cell therapy, progress rapidly due to thecontributions of innovative technologies. Therefore, pioneering new fields often requires the integration of advanced technology,
medicine, and bioscience. In this symposium, we invite prominent researchers who are exploring the frontiers of regenerative
medicine and cell therapy through the implementation of “advancing technologies”. Together, we will discuss the challenges andopportunities presented by these advancing technologies in the development of new medical treatments.

S2-01 AI For Early Disease Pathology Detection

15:30

Bernd Rolauffs

University of Freiburg Medical CenterS2-02 AI Analysis and Immune System Effects

16:00

Melanie L Hart

G.E.R.N. Center for Tissue Replacement, Regeneration & Neogenesis, University of Freiburg Medical
Center, Germany

S2-03 A new treatment strategy combining stem cell therapy and cutting-edge robotic rehabilitation

16:30

Louis Yuge1,2
1

Ken Walker International University, 2Ube MCC Space Regenerative Medicine Center

S2-04 Diversity of Tissue Engineering--from Regenerative Therapy to Food Production--

17:00

Tatsuya Shimizu

Tokyo Women’s Medical University8Day 2 Program Nov. 29 (Wed)
Plenary Lecture 1

9:00~9:40

Lecture Room 1 [141+142]

Chair: Yutaka Miura(Tokyo University of Agriculture and Technology)

PL1

9:00

Understanding the mystery of biological clocks: Learning from unique animals tocontribute to food production and human health
Takashi Yoshimura
Nagoya Univ.

Symposium 3: Autophagy in Food Science10:00~12:00

Lecture Room 1 [141+142]

Organizer: Taichi Hara(Waseda University)
Outline: The symposium focuses on autophagy, a regulated cellular process with potential applications in improving human wellbeing. It aims to explore the societal implications of autophagy and interdisciplinary research in food science. It will also highlightthe efforts of companies and ventures to integrate autophagy into society. By examining challenges and future prospects, thesymposium aims to stimulate meaningful discussions on the broad potential and implications of autophagy for improving humanhealth.

S3-01 Application of autophagy in health and food science

10:00

Taichi Hara
Waseda University

S3-02 Autophagic Activity Evaluation Project Aimed at Extending Healthy Lifespan10:20

Miwako Ishido
AutoPhagyGO Inc.

S3-03 Comparison of Fasting and Non-Fasting Molting on Ovarian function remodelingand transcriptomics in laying hens

10:45

Ling Chang1, Xuexi Zhu1, Kun Xie1,2, Haihan Zhang1, Xi He1, Zehe Song1
1
2

College of Animal Science and Technology, Hunan Agricultural University, China,
Laboratory of Food and Life Science, Faculty of Human Sciences, Waseda University, Japan

S3-04 Spermidine activates mitochondrial trifunctional protein and improves antitumor
11:10
immunity in mice
Kenji Chamoto
Kyoto Univ.

S3-05 Innovation of anthocyanin food from laboratory bench to healthy dining table
11:35

De-Xing Hou
Kagoshima Univ.

9

Luncheon Seminar 1 

12:20~13:10

Lecture Room 1 [141+142]

LS-1
12:20

Introduction of Cell culture related products and services provided by Nichirei
Biosciences

1.

Introduction of Nichirei Biosciences
Nozomi Takahashi
Nichirei Biosciences

2.

Introduction of JSBio Cell Culture Medium (CCM)
Louis Cheung
Jianshun Biosciences Co., Ltd.

Sponsor: NICHIREI BIOSCIENCES INC.
Plenary Lecture 2

13:30~14:10

Lecture Room 1 [141+142]

Chair: Masamichi Kamihira(Kyushu University)

PL2
13:30

Recent advances in genome editing technology and its application in various fields
Takashi Yamamoto
Hiroshima Univ.

Technical Seminars
TS-1
14:25

14:25~16:25

Lecture Room 1 [141+142]

Galactose and Mannose Supplementation in Cell Culture - Glycoform Modulation
and Other Considerations
Sudhakar Voruganti
Pfanstiehl, Inc.

TS-2
14:55

Analysis of Antibody-Drug Conjugate by New High-Performance Hydrophobic
Interaction Chromatography Column
Yosuke Yamasaki
Bioscience Division, Tosoh Corporation, Japan

TS-3-1 Introducing Xcellerex™ X-platform bioreactors
15:10

Carrie Miller
CytivaTS-3-2 Targeted cell culture media manufacturing
15:25

Christopher Woolstenhulme
CytivaTS-4
15:40

BioProfile® FAST CDV and BioProfile® FLEX2 offer various options to monitor yourprocess from the beginning of development to the end of production
Sherry Lin
Nova Biomedical

TS-5
15:55

Novel approach to establish virus inactivation and intermediate polishing processof biological drug
WonHyun Hwang (James)
Avantor Performance Materials
10Symposium 4: Animal Cell Technology from Asia: Toward Bioproduction
16:40~18:40 Lecture Room 1 [141+142]
and Health
Organizer: H iroaki Oda(Nagoya University)
Chairs: Hiroaki Oda(Nagoya University), Shu-Chen Hsieh(National Taiwan University)
Outline: Asia is one of the hottest areas to promote the reserches on animal cell technology. In this symposium, we can share thecutting edge research on the bioproduction and the food and health issutes from Asian colleagues.

S4-01 Development of Chemically Defined, DMSO-free, Serum-free, Animal Componentfree Cryopreservation Media for Mammalian Cells

16:40

Duk Jae Oh
Sejong Univ.

S4-02 Microbeads and 3D printed scaffolds for lab-based meat culture

17:10

Dejian Huang, Linzhi Jing, Lingshan Su, Yan Kong, Han Liu
National University of Singapore

S4-03 Identification of protein targets and proapoptotic mechanism of Chinese olives
extract in colorectal cancer cell lines with thermal stability analysis

17:40

Shu-Chen Hsieh, Guan-Ting Lian
National Taiwan University

S4-04 Anti-inflammatory, Proresolving, and Chemopreventive Effects ofDocosahexaenoic Acid and Its Electrophilic Metabolite

18:10

Young-Joon Surh
Seoul National University

ESACT Medal Ceremony 

18:50~19:05

Lecture Room 1 [141+142]

Poster odd-number

19:15-19:35

Poster Room [Reception Hall]

Poster even-number

19:35-19:55

Poster Room [Reception Hall]*Please present on both Day 2 (11/29) and Day 3 (11/30).

11

Day 3 Program Nov. 30 (Thu)
ESACT Plenary Lecture 1

9:00~9:30

Lecture Room 1 [141+142]

Chair: Takeshi Omasa(Osaka University)

EPL-1 EPIGENETICS AND PHENOTYPES IN CHO cell lines
9:00

Nicole Borth
BOKU University Vienna

ESACT Plenary Lecture 2

9:30~10:00

Lecture Room 1 [141+142]

Chair: Akira Egashira(Astellas Pharma Inc.)

EPL-2 Bispecifics – Different Formats Bring Different Treatment Opportunities But Also
9:30
Different CMC Challenges
Anne Bondgaard Tolstrup
AbtBioConsult ApS

Oral Session 1 

10:18~11:23

Lecture Room 1 [141+142]

Chairs: Yoshinori Kawabe(Kyushu University), Ryo Misaki(Osaka University)

O-1
10:18

Edible Microcarriers From Zein Enable Microtissues Formation For Cell-Based Meat
Linzhi Jing1, Lingshan Su1,2, Dejian Huang1,2, Yan Kong1
1

O-2
10:31

Profiling of Cell Culture Motion for The Standardization and Education
Kengo Momose1, Takeru Shiina1, Yuto Takemoto1, Kei Kanie1,2, Kenjiro Tanaka1, Ryuji Kato1,3
1

O-3
10:44

National University of Singapore (Suzhou) Research Institute , 2National University of Singapore (NUS)

Nagoya Univ., 2Kindai Univ., 3Inst. Nano-life Sys.

Bioprocess Digital Twins: Common Challenges And Solutions
Edward Close
Siemens Digital Industries Software

O-4
10:57

Morphological Profiling of CHO Cells for Detection of Antibody Production
Performance
Takumi Hisada1, Yuto Takemoto1, Kei Kanie1,3, Kenjiro Tanaka1, Ryuji Kato1,2
1

O-5
11:10

Nagoya Univ., 2Inst. Nano-life Sys., 3Kindai Univ.

Understanding of Morphological Data Landscape for Effective Data Augmentation
Kazue Kimura1, Yuto Takemoto1, Kanie Kei1,2, Kenjiro Tanaka1, Ryuji Kato1,3
1

Graduate School of Pharmaceutical Sciences, Nagoya University, Japan,
Faculty of Engineering Graduate School of Systems Engineering, Kindai University, Japan,
3
Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University212Oral Session 2 

10:05~11:36

Lecture Room 2 [131+132]

Chairs: Chuda Chittasupho(Chiang Mai University), Meng-Chieh Hsu(National Taiwan University)

O-6

10:05

Promotion of Skin Epidermal Formation in Three-dimensional Culture System by
Shaking Treatment Through Reduction of Hypoxia Response
Mayuko Endo, Kojin Kitadani, Hirofumi Teshima, Kiyotaka Hitomi
Grad. Sch. Pharm. Sci., Nagoya Univ.

O-7

10:18

Evaluation of Angiogenic Potency of Human Mesenchymal Stem Cell Using 5-layer
Skeletal Muscle Cell Sheet
Megumi Fukuda1, Rikiya Nitta1, Parichut Thummarati1,2, Masahiro Kino-oka1,31

Dept. Biotechnology, Grad. Sch. Eng., Osaka University, Japan,
Center Excellent for Biosensors and Bioengineering(CEBB), Dept. Clin. Chem., Fac. Allied Health Sci.,
Chulalongkorn University, Thailand,3
Research Base for Cell Manufacturabillity, Grad. Sch. Eng., Osaka University, Japan
2

O-8
10:31

Phenylethanoid Glycoside-Enriched Extract Prepared from Clerodendrum
chinense Leaf Inhibits A549 Lung Cancer Cell Migration and Apoptosis Induction
through Enhancing ROS Production
Chuda Chittasupho1, Sirivan Athikomkulchai2, Weerasak Samee3, Mingkwan NaTakuathung4,5,
Wipawadee Yooin1,6, Kasirawat Sawangrat1,6, Chalermpong Saenjum1,61

Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Thailand,
Department of Pharmacognosy, Faculty of Pharmacy, Srinakharinwirot University, Thailand,
3
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Srinakharinwirot University, Thailand,
4
Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Thailand,5
Clinical Research Center for Food and Herbal Product Trials and Development (CR-FAH), Faculty of
Medicine, Chiang Mai University, Thailand,
6
Center of Excellence for Innovation in Analytical Science and Technology for Biodiversity-Based
Economic and Society (I-ANALY-S-T_B.BES-CMU), Chiang Mai University, Thailand
2

O-9
10:44

Anti-inflammatory Effect of Clove Aqueous Extract on LPS-stimulated Macrophage
Cell Line RAW 264.7 Cells
Sellen Gurusmatika1, Momoko Ishida1, Kosuke Nishi1, Hiroyuki Onda2, Nanami Yoshino2,
Takuya Sugahara1
1

O-10
10:57

Ehime University, 2S&B FOODS INC

Anserine Suppresses High-Fat Diet-Induced Inflammation in Intestinal Tissues
Arisa Yoshida1, Yimei Wang1, Miyuki Shiobara1, Kohei Soga1, Yusuke Saeki1, Shuhei Kodera1,
Hibine Mizobuchi1, Chenxu Lei2, Kenichiro Sato3, Yoshinori Katakura4, Mamoru Totsuka5,
Tatsuhiro Hisatsune2, Haruyo Nakajima-Adachi1, Satoshi Hachimura1
1

Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, University of Tokyo,
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo,
3
Tokai Bussan, Co., 4Faculty of Agriculture, Kyushu University,
5
Department of Molecular Microbiology, Faculty of Life Science, Tokyo University of Agriculture
2

O-11
11:10

Mitochondrial Quality Control: The Import Stress Response
Meng-Chieh Hsu1,2, Richard Youle2
1

National Taiwan University, Taiwan, 2National Institutes of Health, USA

13

O-12
11:23

Immortalization of primary cells derived from the endangered Ryukyu long-furred rat
Lanlan Bai1, Noe Kikuchi1, Takahiro Eitsuka2, Himari Matsusaka1, Kiyotaka Nakagawa2,
Masafumi Katayama3, Keiko Ito4, Miho Inoue-Murayama5, Tohru Kiyono6, Tomokazu Fukuda1
1

Graduate School of Science and Engineering, Iwate University, Japan,
Graduate School of Agricultural Science, Tohoku University, Japan,
3
Environmental Genomics Office, Biodiversity Division, National Institute for Environmental Studies, Japan,
4
Amami Dog and Cat Animal Hospital, Japan, 5Wildlife Research Center, Kyoto University, Japan,
6
Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Japan.
2

Luncheon Seminar 2
LS-2
11:50

11:50~12:40

Lecture Room 1 [141+142]

Usage of Raman for process control
Henry Weichert
Sartorius Stedim Biotech GmbH, Goettingen

Sponsor: Sartorius Stedim Biotech GmbH
Symposium 5: “PostCas9” Genome Editing - Paving the Way for
13:00~15:00 Lecture Room 1 [141+142]
Industrial Applications 
Organizers: Tetsushi Sakuma(Hiroshima University), Kazuto Yoshimi(The University of Tokyo)
Co-hosted by The Committee for Education and Training, The Japanese Society for Genome Editing
Outline: After the demonstration of genome editing in mammalian cells using CRISPR-Cas9, it has become an essential technology
for animal cell engineering in this decade. CRISPR-Cas9 is easy to manipulate and shows high genome editing efficiency;
however, alternative technologies other than CRISPR-Cas9 are also quite important especially for the industrial applications.
In this symposium, we focus on such an alternative toolbox enabling industry-friendly gene engineering in animal cells. Fivesymposiasts cover the topics not only for genome editing tools but also for transcriptome editing and bioinformatics tools.

Opening Remarks
13:00

S5-01 Genome editing technology and applications with the type I-E CRISPR system
13:02

Kazuto Yoshimi
Institute of Medical Science, The University of Tokyo

S5-02 Development of a novel genome editing technology using CRISPR-Cas type I-D
13:25
toward its industrial application
Keishi Osakabe
Tokushima Univ.

S5-03 CRISPR-free Nuclear Base Editing by the Cooperation of Novel Single-molecule
13:48
Nickase and Deaminase Fused with TAL Effectors
Tetsushi Sakuma1, Nahoko Nishibori2, Hina Kubota3, Tadahiko Yoshima3
1

Graduate School of Integrated Sciences for Life, Hiroshima University,
Genome Editing Innovation Center, Hiroshima University,
3
Bioscience Research Laboratory, Sumitomo Chemical Co., Ltd
2

S5-04 Computational Resources for Industrial Application of Genome Editing
14:11

Kazuki Nakamae1,2
1

Hiroshima Univ., 2PtBio Inc.

14

S5-05 PPR Protein-Based Transcriptome Editing

14:34

Takahiro Nakamura
Kyushu Univ.

Closing Remarks

14:57

Symposium 6: Recent Advances in Cell Culture and Cell Engineering (I)
15:30~17:30 Lecture Room 1 [141+142]Organizers: Takeshi Omasa(Osaka University), Akira Egashira(Astellas Pharma Inc.)
Outline: Focusing on the recent progress in cell engineering and cell culture engineering, this symposium aims to introduce thelatest findings from Japanese and Asian companies and academia and to discuss future directions. We also would like to discuss
the global networks among industry and academia and education, training, and human resources.

Opening Remarks

15:30

S6-01 Development of a stable antibody production system utilizing an Hspa5 promoterin CHO cells

15:34

Hiroki Tanemura1, Kenji Masuda1, Takeshi Okumura1, Eri Takagi1, Daisuke Kajihara1,
Hirofumi Kakihara1, Koichi Nonaka1, Ryo Ushioda2
1

DAIICHI SANKYO CO., LTD., 2Kyoto Sangyo University

S6-02 Application of Multi-omics Profiling and Design of Experiment (DoE) Approach in
16:03
Biologics Process Development
Say Kong Ng, Evan Tan, Alison Lee, Ying Swan Ho
Bioprocessing Technology Institute/ A*STAR

S6-03 Non-Natively Structured Antibodies in CHO Bioprocessing
16:32

Masayoshi Onitsuka
Tokushima Univ.

S6-04 Cellvento® ModiFeed Program:Tailoring Protein Critical Quality Attributes with
17:01
Feeds
Sadao Ozawa1, Martina Grabner2, Kate Hellman3
1

Merck Limited, Japan, 2Merck KGaA, Germany, 3MilliporeSigma, USA.

Poster odd-number

17:45-18:10

Poster Room [Reception Hall]

Poster even-number

18:10-18:35

Poster Room [Reception Hall]*Please present on both Day 2 (11/29) and Day 3 (11/30).

15

Day 4 Program Dec. 1 (Fri)Plenary Lecture 3

9:00~9:40

Lecture Room 1 [141+142]

Chair: Kiyotaka Hitomi(Nagoya University)

PL3
9:00

Advancements in Regenerative Medicine for Ophthalmology
Takahiro Ogasawara
Japan Tissue Engineering Co., Ltd.

Symposium 7: Recent Advances in Cell Culture and Cell Engineering (II)9:50~11:50 Lecture Room 1 [141+142]Organizers: Takeshi Omasa(Osaka University), Akira Egashira(Astellas Pharma Inc.)
Outline: Focusing on the recent progress in cell engineering and cell culture engineering, this symposium aims to introduce thelatest findings from Japanese and Asian companies and academia and to discuss future directions. We also would like to discuss
the global networks among industry and academia and education, training, and human resources.

S7-01 Innovative Technologies Enabling Efficient Development and Manufacturing of
9:50
Multiple Formats of Bispecific Antibodies
Weichang Zhou
WuXi Biologics

S7-02 Cystine And Tyrosine Feed Reduces Oxidative And ER Stress In CHO Cells
10:19

Yusuke Shibafuji1, Nobuyoshi Nagao1, Masafumi Yohda2
1

AGC Inc., 2Tokyo University of Agriculture and Technology

S7-03 Development of a real time monitoring method for animal cell culture processes
10:48
with Raman spectroscopy
Yuichi Matsumoto
Kyowa Kirin Co.,Ltd.

S7-04 Using N-1 perfusion and compacted media intensifies the seed train
11:17

Sadao Ozawa
Merck Limited, Japan

Closing Remarks
11:46

Luncheon Seminar 3
LS-3
12:10

12:10~13:00

Lecture Room 1 [141+142]

Seed train intensification with perfusion technology - Cost Reduction and Improve
Throughput
Sadao Ozawa
Merck Limited, Japan

Sponsor: Merck Ltd.

16

Oral Session 3 

13:15~14:59

Lecture Room 1 [141+142]

Chairs: Hirokazu Akiyama(Nagoya University), Masahiro Kino-oka(Osaka University)

O-13

13:15

Manufacturing Scale Demonstration Of A Continuous Biomanufacturing Process
For Antibody Production
Atsushi Inada1, Hiroshi Sakuyama1, Nobuyuki Haraguchi1, Takashi Kurosawa1, Masayuki Haga1,
Katsuhiro Shimono1, Junichi Kori1, Shuhei Katayama1, Tatsuya Matsuura1, Yoichi Nagai1,
Shunichi Yoshida1, Kosuke Taniguchi1, Naomichi Hikichi1, Leon Pybus2, John Raven2,
Tibor Nagy2, Charles Heise2, Michelle Lyons2, Shinichi Nakai1
1

O-14
13:28

FUJIFILM Corporation, 2FUJIFILM Diosynth Biotechnologies UK Limited

Evaluation of ambr250 as a scale down model in upstream process development
at Takeda
Hayato Koizumi
Takeda Pharmaceutical Company

O-15
13:41

Production of bio heparins using engineered CHO cells
Daiki Okada, Yoshinori Kawabe, Razia Sultana, Feiyang Zheng, Yuki Amamoto,
Masamichi Kamihira
Kyushu Univ.

O-16
13:54

Elucidation of a 3D Primary Culture System of Human Mesenchymal Stem Cells
Masashi Okitani1, Riku Yamamoto1, Kenichi Yamahara3, Akiko Hamada3, Masahiro Kino-oka1,2
1

Dept. Biotechnology, Grad. Sch. Eng., Osaka University, Japan,
Research Base for Cell Manufacturability, Grad. Sch. Eng., Osaka University, Japan,
3
Laboratory of Molecular and Cellular Therapy, Institute for Advanced Medical Sciences, Hyogo Medical
University, Japan
2

O-17
14:07

Development of a Suspension Culture System for MSCs on a Fluffy Scaffold
Gabrielle Erwin Gemeniano Awitan1, Riku Yamamoto1, Masahiro Kino-oka1,2
1
2

O-18
14:20

Dept. Biotechnology, Grad. Sch. Eng., Osaka University, Japan,
Research Base for Cell Manufacturability, Grad. Sch. Eng., Osaka University, Japan

Expansion of Human Mesenchymal Stem Cells Using Collagen-modified Polyvinyl
Alcohol Microcarriers
Masahiro Kaneko1, Airi Sato2, Satoru Ayano3, Akio Fujita3, Goro Kobayashi3, Akira Ito1
1

O-19
14:33

Nagoya Univ., 2Chubu Univ., 3Kuraray Co., Ltd.

Process Optimization of Multipotent Cardiovascular Progenitor Cell Generationfrom iPSCs using Design of Experiments
Hirokazu Akiyama, Yosuke Katayama, Kazunori Shimizu, Hiroyuki Honda
Nagoya University

O-20
14:46

E-Cadherin Mediated Regulation of iPSC Fates toward Functional Hepatocytes
using Botulinum Hemagglutinin
Mee-Hae Kim1, Masahiro Kino-oka1,2
12

Department of Biotechnology, Osaka University,
Research Base for Cell Manufacturability, Osaka University

17Oral Session 4 

13:15~15:12

Lecture Room 2 [131+132]

Chairs: Farhana Ferdousi(University of Tsukuba), Masahiro Kawahara(NIBIOHN)

O-21
13:15

Somatic Differentiation of Chicken Primordial Germ Cells Exhibits Partial
Epithelial-to-Mesenchymal Transition (EMT)-like Features
Kennosuke Ichikawa1, Yuzuha Motoe2, Mei Matsuzaki2, Ryo Ezaki2, Hiroyuki Horiuchi2,
Mike McGrew1
1

O-22
13:28

The University of Edinburgh, UK, 2Hiroshima University, Japan

4,4'-Dimethoxychalcone (DMC): A Novel GATA2-Dependent Modulator ofAutophagy Beyond mTOR Signaling
Jinyun Wang1, Kun Xie1,2, Saki Shiota1, Miyu Kamimura1, Taichi Hara1
1

O-23
13:41

Waseda Univ., 2Hunan Agricultural Univ.

Autophagy Induction by D-Tryptophan and D-Aspartic Acid: A Dive into mTORindependent Signaling Pathways
Kun Xie1,2, Jinyun Wang1, Miyu Kamimura1, Saki Shiota1, Taichi Hara1

1
2

O-24
13:54

Laboratory of Food and Life Science, Faculty of Human Sciences, Waseda University, Japan,
College of Animal Science and Technology, Hunan Agricultural University, China

The Relationship Between the Ingredient Composition in Chenpi Extracts andTheir Physiological Effects
Nao Kumitake1, Sakurako Hamada2, Ryosuke Fujinuma2, Shiho Kagami3, Masaaki Yamada3,
Chie Umatani1, Yutaka Miura1
1

Division of Applied Biological Chemistry, Institute of Agriculture, Tokyo University of Agriculture andTechnology,2
Department of Natural Science, College of Liberal Arts, International Christian University,
3
Division of International Environmental and Agricultural Science, Institute of Agriculture, Tokyo
University of Agriculture and Technology

O-25
14:07

Mechanism of Taurine on Improving Hypercholesterolemia Using Transcriptomics
Qi Song, Satoru Kobayashi, Hiroaki Oda
Nagoya Univ.

O-26
14:20

Ashitaba Chalcones Prevent Hyperglycemia via AMPK-dependent Glucose Uptake
in Skeletal Muscle Cells and GLP-1 Secretion from Enteroendocrine L-Cells
Kevin Odongo1, Ayane Abe1, Moe Ishinaka1, Rina Kawasaki1, Naoki Harada2,
Kyuichi Kawabata3, Hitoshi Ashida1
1Graduate School of Agricultural Science, Kobe University, Japan,

2

Graduate School of Agriculture, Osaka Metropolitan University, Japan,
Faculty of Clinical Nutrition and Dietetics, Konan Women's University, Japan

3

O-27
14:33

A Whole-transcriptomic Study Using a Stem Cell-Based Model for Comparing
the Multi-tissue-specific Bioactivities of Rare Olive Compound Oleacein and
Oleocanthal
Farhana Ferdousi1,2, Kazunori Sasaki2,3, Ken-ichi Tominaga3, Hiroko Isoda1,2,3
1

Institute of Life and Environmental Sciences, University of Tsukuba,
Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba,
3
Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), National
Institute of Advanced Industrial Science and Technology (AIST)
2

18

O-28

14:46

Construction of the designer c-KIT for arbitrarily controlling signal transduction
properties
Masahiro Kawahara1,2, Tatphon Kongkrongtong2
1

O-29

14:59

NIBIOHN, 2The University of Tokyo

Regulation of NAGNAG Acceptor Alternative Splicing in the Clock Gene RORα andIts Effect on Clock Oscillation
Naho Hashimoto, Shunsuke Nakajima, Yuki Hosaka, Hiroaki Oda
Nagoya Univ.

Award Ceremony / Closing Remarks

19

15:30~15:45

Lecture Room 1 [141+142]

Poster Session
Poster Session

Poster Room [Reception Hall]

Nov. 29 19:15-19:35 (Poster odd-number)
19:40-19:55 (Poster even-number)
Nov. 30 17:45-18:10 (Poster odd-number)
18:10-18:35 (Poster even-number)*Please present on both Day 2 (11/29) and Day 3 (11/30).

P-01

Automating subunit mAb attribute screening for in-process monitoring of an
automated high-throughput multi-parallel bioreactor
Thanai Paxton1, Kenji Hirose1, Nick Pittman2, Patrick Boyce2, Caitlin Hanna3,
Samantha Ippoliti3, Yun W Alelyunas3, Charles Prochaska4, Clint Kukla4, Guillaume Bechade2,
Magnus Wetterhall2, Mark Wrona3, Stephan M Koza3, Ying Qing Yu3
1

Nihon Waters K.K., 2Waters Corporation, United Kingdom, 3Waters Corporation, MA,
4
Sartorius Stedim NA, NY

P-02

Characteristics of transfection reagents for obtaining high rAAV vector production
Kyoko Masumi-Koizumi, Keisuke Yusa, Kazuhisa Uchida
Kobe Univ.

P-03

Evaluation of Next-Generation MAb Production Platform Consisting of CHO-MK
Cells and Customized Media
Hisashi Saeki, Kaori Fueki, Naoki Maeda
FUJIFILM Wako Pure Chemical Corporation

P-04

Current status/issues of raw material management and efforts toward stableproduction of Biopharmaceuticals drug substance manufacturing (cell culture
stream)Megumi Sugiyama, Kentaro Sakai, Ken Takahashi, Naoki Matsunaga, Ryuji Nomura
Kyowa Kirin Co., Ltd

P-05

PEPTIDE-BASED-SOLUTIONS TO REDUCE UNDESIRED CELL CULTURE MEDIACHEMISTRY – NEW OPTIONS FOR STABILIZED MEDIA FORMULATIONS
Spandan Mishra1, Stephan Brinkmann2, Martin Schilling2, Christina Jost2, Tamara Heinze2,
Risai Dubrall2, Anne Benedikt2
1

P-06

Evonik (Sea) Pte. Ltd., 2Evonik Operations GmbH

Copper Precipitation Associated with the Formation of Hydrogen Sulfide During
Cell Culture Media Preparation
Hisashi Saeki, Naoki Maeda, Sadamu Kurono
FUJIFILM Wako Pure Chemical Corporation

P-07

Myricetin protects cognitive function accompanied with upregulation of BDNF
expression
Yu Shimada, Yuka Sato, Ryo Kitamura, Motofumi Kumazoe, Yoshinori Fujimura,
Hirofumi Tachibana
Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty ofAgriculture, Kyushu University

20

P-08

Risk assessment for biopharmaceutical single-use manufacturing: a case study of
upstream continuous processing
Takao Ito1, Hui Wang2, Soon-Hwa Hwang3, Bin Wang2, Lizhi Wang2, Somasundaram G4
1

P-09

Merck Ltd. Japan, 2Merck Chemicals (Shanghai) Co Ltd., 3Merck Ltd. Korea, 4Merck Pte. Ltd

Comprehensive transcriptome data to identify downstream genes of testosterone
signalling in dermal papilla cells
Himari Matsusaka1, Tao Wu1, Tomoe Yamada Kato2, Lanlan Bai1, Hiroshi Tomita1,
Eriko Sugano1, Taku Ozaki1, Tohru Kiyono3, Isao Okunishi2, Tomokazu Fukuda1
1

Graduate School of Science and Engineering, Iwate University, Japan,
Research & Development Division, Kinjirushi Company Limited, Japan,
3
Exploratory Oncology Research and Clinical Trial Center, Japan
2

P-10

Do More With Less: Fit-For-Purpose Tools To Speed Up upstream Process
Development For Continuous Biomanufacturing
Yusuke Tomioka1, Quang Long Pham2, Channing McLaurin2, Jana Mahadevan2
1
2

P-11

Life science, Process solutions, Merck Ltd, Japan,
Innovation Cell Culture Media Development, Life Science, Process solutions, Merck KGaA, Germany.

Development of CO2-independent Media for iPSCs Expansion and Cardiomyocyte
Differentiation
Yugo Okazaki1, Tomoaki Kato2, Yuko Kitano2, Kenji Yoshimochi1, Motoshi Shimotsuma1,
Masoyoshi Tsukahara2
1

P-12

Nacalai Tesque, 2CiRA Foundation

Development of new microcarriers suitable for mass culture of therapeutic cells
Yusuke Hirai, Satoru Ayano, Akio Fujita, Goro Kobayashi
KURARAY CO., LTD

P-13

Studies on Anti-degranulation Activity of Flaxseed Water-soluble Dietary Fiber
Extract
Zixuan Teng1, Kosuke Nishi1,2, Momoko Ishida1,2, Kyoko Shimazu3, Satoshi Fukumitsu3,
Takuya Sugahara1,2
1

Graduate School of Agriculture, Ehime University, Japan,
Food and Health Function Research Center, Ehime University, Japan,
3
Central Research Laboratory, Innovation Center, NIPPN Corporation, Japan2

P-14

Development and optimization of CRISPR-Cas3-mediated gene knock-in technology
Daiki Nagatomo1, Kazuto Yoshimi2, Tomoji Mashimo2, Tetsushi Sakuma1
1
2

P-15

Graduate School of Integrated Sciences for Life, Hiroshima University,
Institute of Medical Science, The University of Tokyo

Expansion of the LoAD system for efficient genome engineering by strongly
biasing the editing outcomes
Sota Nishikawa, Mizuki Sato, Tetsushi Sakuma
Graduate School of Integrated Sciences for Life, Hiroshima University

P-16

CRISPRaic: simultaneous, orthogonal, and high-efficiency control of the activation
and repression of transcription and DNA cleavage
Shunichiro Usui1, Atsushi Kunii2, Tetsushi Sakuma1
1

Grad. Sch. Integrat. Sci. for Life, Hiroshima Univ., 2Grad. Sch. Sci., Hiroshima Univ.
21

P-17

Development of a system to select high antibody-producing cells using the CELL
HANDLERTM cell picking & imaging system
Gakuro Harada1, Emiko Matsubara1, Hiroshi Tahara2, Kazuya Tsujioka2, Kiyotaka Matsuno1,
Yuichi Hikichi1
1

P-18

Yamaha Motor Co., Ltd., 2FUSO Pharmaceutical Industries, Ltd.

Medium optimization for mammalian cells using machine learning
Takamasa Hashizume, Yuki Ozawa, Bei-Wen Ying
School of Life and Environmental Sciences, University of Tsukuba

P-19

Enhancement of Antibody Productivity and Cell Viability Improvement by
Ubenimex as a Media Additive of CHO Fed-batch Culture
Yosuke Wada, Yasunori Oba, Yoshifumi Kishi, Ken Shimada, Yusuke Akashi, Hitomi Manaka,
Tomotake Takai, Shunsuke Ohira
Astellas Pharma Inc.

P-20

Development of a high-performance transcriptional activation system using Class
1 CRISPR system
Maya Oko1, Atsushi Kunii2, Kazuto Yoshimi3, Tomoji Mashimo3, Tetsushi Sakuma1

P-21

1

Graduate School of Integrated Sciences for Life, Hiroshima University,

2

Graduate School of Science, Hiroshima University, 3Institute of Medical Science, The University of Tokyo

New High-Performance Hydrophobic Interaction Chromatography Column for
Separation of Antibody-Drug Conjugate (ADC)
Yosuke Yamasaki, Toshitaka Ohmori, Yukie Hamada, Hidetaka Kobayashi, Hiroshi Tomizawa,
Kazuaki Muranaka
Tosoh Corporation

P-22

Application of next-generation sequencing for virus detection in thebiopharmaceutical manufacturing process
Dichi Fujiawa1, Noriko Hashiba2, Keina Yamaguchi1, Keisuke Yusa2, Kousuke Kuroda1,
Kazuhisa Uchida2
1

P-23

Kyowa Kirin Co., Ltd., 2Kobe University

Effect of water-soluble fraction of a heated water extract of defatted Perillafrutescens Britton var. japonica Hara seed residue on the regulation of bonemetabolism
Hiroyuki Asano1, Kitaji Masataka2, Shunma Sato2, Sogo Nishimoto1,2

1
2

P-24

Graduate school of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Japan,
Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Japan

Anti-inflammatory effect of Sake Lees Free Ceramide in Lipopolysaccharide (LPS)stimulated RAW 264.7 Macrophages
Hiroko Okuda1, Hiroyuki Asano1, Yoshinori Umezawa2, Sogo Nishimoto1,3
1

Graduate School of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Japan,
Fukumitsuya Sake Brewery, Japan,
3
Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Japan
2

22

P-25

Development of the transgenic chicken constitutively-expressing Streptococcus
pyogenes Cas9
Yukiko Kondo, Yuya Okuzaki, Ken-ichi Nishijima
Nagoya Univ.

P-26

Exploring and Identification of Functional Inhibitors for Human ABCC11, a Risk
Factor of Axillary Osmidrosis
Yu Toyoda1,2, Hirotaka Matsuo2, Tappei Takada1

1

P-27

The University of Tokyo Hospital, 2National Defense Medical College

Assessing the Impact of Cell Culture Conditions on Recombinant Antibody
Production in CHO Perfusion Culture
Muneyoshi Okamoto1, Hiroaki Kato2, Keisuke Shibuya3, Hiroe Amo4, Masayoshi Onitsuka4
1

Grad. Sch. Sci. Technol. Innov., Tokushima Univ.,
Res. Dev. Dept. Innov. Div., Hitachi Plant Services Co., Ltd., 3Res. Dev., Hitachi, Ltd.,
4
Grad. Sch. Biosci. Bioind, Tokushima Univ.
2

P-28

3D-Printed Prolamin Scaffolds for Cell-Based Meat Culture
Lingshan Su, Linzhi Jing, Dejian Huang
National University of Singapore

P-29

Novel Manufacturing Process for Active Human Hepatocyte Growth Factor (hHGF)
in Animal Component-free Condition
Masashi Shimizu1, Toshitaka Sato1, Mami Gomibuchi1, Sachiko Kikuchi2, Yoshifumi Uemoto1
1

P-30

Eisai Co., Ltd., 2EA Pharma Co., Ltd.

Development of a novel screening system for an anti-aging food targeting FOXO
Jiahao Wang, Yoshinori Katakura, Miyako Udono, Ayano Tanaka
Kyushu Univ.

P-31

Activation of muscle-brain interaction by carnosine
Momoka Sato1, Asuka Ishibashi1, Miyako Udono1, Mikako Sato2, Yoshinori Katakura1
1

P-32

Kyushu Univ., 2Research & Development Center, NH Foods

Exploring the Anti-adipogenic Potential of Tsuruazuki Bean Extracts in 3T3-L1
Preadipocytes
Elda Nurafnie Ibnu Rasid1, Farhana Ferdousi1,2,3, Shinya Takahashi1,2,3, Norihiko Tomooka4,
Kyoko Toda4, Mari Maeda-Yamamoto4, Hiroko Isoda1,2,3,5
1

Tsukuba Life Science Innovation (T-LSI) Program, Graduate School of Science and Technology, Universityof Tsukuba, Japan,
2
Institute of Life and Environmental Sciences, University of Tsukuba, Japan,
3
Alliance for Research on North Africa (ARENA), University of Tsukuba, Japan,
4
National Agriculture and Food Research Organization, Japan,
5Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), National
Institute of Advanced Industrial Science and Technology (AIST), Japan

23

P-33

Ethylene Glycol Derivatives of Squalene Alleviate Excessive Lipogenesis
and Inflammatory Response in 3T3-L1 Preadipocytes: A Comparative WholeTranscriptomics Study
Yu Cheng1, Farhana Ferdousi1,2,3,4, Bryan Angelo Foronda4, Tran Ngoc Linh5,
Munkhzul Ganbold5, Akira Yada5,6, Takashi Arimura5, Hiroko Isoda1,2,3,4,5
1

Tsukuba Life Science Innovation (T-LSI) program, Graduate School of Science and Technology, Universityof Tsukuba,
2
Institute of Life and Environmental Sciences, University of Tsukuba,
3
Alliance of Research on the Mediterranean and North Africa (ARENA), University of Tsukuba,
4
School of Life and Environmental Sciences, University of Tsukuba,
5Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), National
Institute of Advanced Industrial Science and Technology (AIST),
6Interdisciplinary Research Center for Catalytic Chemistry, National Institute of Advanced Industrial
Science and Technology (AIST)P-34

A Comparative Study to Understand the Molecular Mechanisms Contributing to
the Enhanced Neurogenesis Effects of Caffeoylquinic and Feruloylquinic Acid
Isomers in Adult Mice Neural Stem Cells
Hongyu Lin1, Kazunori Sasaki2,3, Farhana Ferdousi1,3,4, Masataka Hatanaka2,
Ken-ichi Tominaga1,2, Hiroko Isoda1,2,3,4
1

Tsukuba Life Science Innovation (T-LSI) Program, Graduate School of Science and Technology, Universityof Tsukuba, Japan,
2Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), National
Institute of Advanced Industrial Science and Technology (AIST), Japan,
3
Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, Japan,
4
Institute of Life and Environmental Sciences, University of Tsukuba, Japan

P-35

Development of CHO Cells Harboring Production Enhancer Genes (PEGs)
Nanako Fukuma1, Seiji Yamauchi2, Koji Tajino2, Masayoshi Onitsuka3
1

Grad. Sch. Sci. Technol. Innov., Tokushima Univ., 2chromocenter Inc.,
3
Grad. Sch. Biosci. Bioind, Tokushima Univ.

P-36

Hepatocyte Spheroid Culture using Two Microwell Chips having PEG or MicoCellTM
Surfaces
Sae Yokomine1, Tomomi Makino2, Emiko Nagao2, Kohji Nakazawa1
1

P-37

The University of Kitakyushu, 2NIPPON SHOKUBAI CO., LTD.

A Newly Synthesized Amphiphilic Squalene Derivative Exerts Anti-Metastatic
Activity in Malignant Melanoma Cells
Yaman Zhang1, Meriem Bejaoui2,3, Tran Ngoc Linh2, Takashi Arimura2, Hiroko Isoda1,2,3,4
1

Tsukuba Life Science Innovation (T-LSI) Program, Graduate School of Science and Technology, Universityof Tsukuba, Japan,
2Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), National
Institute of Advanced Industrial Science and Technology (AIST), Japan,
3
Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, Japan,
4
Institution of Life and Environmental Sciences, University of Tsukuba, Japan

24

P-38

Generation and Phenotype Analysis of Conditional Knockout Mice Deficient inTransglutaminase 1, a Protein Cross-linking Enzyme That Regulates Epithelial
Barrier Function
Atsuki Takahashi, Ryota Tamura, Hideki Tatsukawa, Kiyotaka Hitomi
Nagoya Univ.

P-39

Hepatic Maturation of Differentiating iPS Cells in a Three-Dimensional Culture
Using Hollow Fibers
Yuki Hokamaki1, Hiroshi Mizumoto2, Toshihisa Kajiwara2
1
2

P-40

Graduate School of Engineering, Kyushu University,
Department of Chemical Engineering, Faculty of Engineering, Kyushu University

Employ active learning to develop the culture medium for increased cellconcentration
Yuki Ozawa, Takamasa Hashizume, Bei-Wen Ying
School of Life and Environmental Sciences, University of Tsukuba

P-41

Maximizing IgG Titer of the Mammalian Cell Cultivation Process throughOptimization of the Metabolic Product Contents in the Media using Artificial
Intelligence/Machine Learning
Jooyang Park, Jiyong Yoon, Jeonghyuk Park
PRESTIGE BIOLOGICS

P-42

Profiling of the N-linked glycans of IgG produced by CHL-YN cells
Hirotaka Kuroda1,2,3, Akari Noma1, Mayu Okajima2, Kazuya Sorada1, Noriko Yamano-Adachi1,
Junko Iida2,3, Takeshi Omasa1
1P-43

Grad. Sch. Eng., Osaka Univ., 2Shimadzu Corp., 3Shimadzu Analytical Innovation Research Lab.

Knockout of Chicken Fucosyltransferase 8 Towards The Production System of
Pharmaceutical Antibodies
Kohei Fujiwara, Yuya Okuzaki, Ken-ichi Nishijima
Nagoya Univ.

P-44

The metabolic analysis of CHL-YN cell through the analysis of organic / inorganic
components in culture supernatant
Akari Noma1, Hirotaka Kuroda1,2,3, Kazuya Sorada1, Noriko Yamano- Adachi1, Junko Iida2,3,
Takeshi Omasa1
1P-45

Grad. Sch. Eng., Osaka Univ., 2Shimadzu Corp., 3Shimadzu Analytical Innovation Research Lab.

Global Transcriptome Analysis Reveals the Potential Role of Oleacein in Lipid andGlucose Metabolism and Inflammation in Adipocytes Derived from Healthy andDiabetic Adipose Stem Cells
Rui Wang1, Munkhzul Ganbold2, Farhana Ferdousi1,3,4, Kenichi Tominaga1,2, Hiroko Isoda1,2,3,4
1

Tsukuba Life Science Innovation (T-LSI) Program, Graduate School of Science and Technology, Universityof Tsukuba, Japan,
2Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), National
Institute of Advanced Industrial Science and Technology (AIST), Japan,
3
Institute of Life and Environmental Sciences, University of Tsukuba, Japan,
4
Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, Japan

25

P-46

Antibody Characterization Utilizing FcRn Affinity Chromatography
Tatsuya Yumoto, Naruaki Inoue, Ryuma Ikeura, Yosuke Terao
Tosoh Co., Ltd.

P-47

Monitoring Inline Viable Cell Density and Optimizing N-1 Perfusion Culture of CHO
Cells with a Capacitance Sensor
Kouki Kuroda, Benjamin Bayer, Gioia Tuozzolo
Takeda Pharmaceutical Company Ltd.

P-48

The Strategy to Shorten the Cell Line Development Platform using CHO-MK cells
Mizuki Morisasa1, Junshin Iwabuchi1,2, Takayuki Horiuchi1,21P-49

Chitose Laboratory Corp., 2Manufacturing Technology Association of Biologics

Process Optimization For CHO Cell Culture Using DoE Approach In Ambr15
Miroslav Matev, Shiori Akiyama, Toru Tsuji, Satoshi Igarashi
Sartorius Stedim Japan K.K.

P-50

An Approach for Uniform Cell Growth in a Three-dimensional Culture of iPS Cells
Using Hollow Fibers
Kenta Tamura1, Hiroshi Mizumoto2, Toshihisa Kajiwara2
1
2

P-51

Graduate School of Engineering, Kyushu University,
Department of Chemical Engineering, Faculty of Engineering, Kyushu University

Effect of Culture Oxygen Environment on In Vitro Cartilage Differentiation
Risa Kawasaki, Kohji Nakazawa
The Univ. of Kitakyushu

P-52

Perfusion culture of Chinese hamster lung (CHL)-YN cells in glutamine-free
medium
Sayaka Nagae1, Hiromu Kunita1, Noriko Yamano-Adachi1,2, Takeshi Omasa1,2
1

P-53

Graduate School of Engineering, Osaka University, Japan, 2OTRI, Osaka University, Japan

Hair Loss Preventing Potential of Botryococcus terribilis, Methylated-Meijicoccene- A Novel Bioactive Compound, And C32 Botryococcene
Aprill Kee Oliva Mizushima1,2,3, Meriem Bejaoui3,4, Atsushi Hirano5, Takashi Arimura4,
Tran Ngoc Linh4, Eriko Uchiage4, Sachiko Nukaga5, Kenichi Tominaga4, Hiroyuki Nozaki5,
Hiroko Isoda1,2,3,4,6
1

Tsukuba Life Science Innovation (T-LSI) Program, Graduate School of Science and Technology, Universityof Tsukuba, Japan,
2
Research and Development Center for Tailor-made QOL Program, University of Tsukuba, Japan,
3
Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, Japan,
4Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), National
Institute of Advanced Industrial Science and Technology (AIST), Japan,
5Tokyo Electric Power Company Holdings, Inc., Japan,
6
Institute of Life and Environmental Sciences, University of Tsukuba, Japan

26

P-54

Manufacturing Scale Demonstration Of A Continuous Biomanufacturing Process
For Antibody Production
Hiroshi Sakuyama1, Atsushi Inada1, Nobuyuki Haraguchi1, Takashi Kurosawa1, Masayuki Haga1,
Katsuhiro Shimono1, Junichi Kori1, Shuhei Katayama1, Tatsuya Matsuura1, Yoichi Nagai1,
Shunichi Yoshida1, Kosuke Taniguchi1, Naomichi Hikichi1, Leon Pybus2, John Raven2,
Tibor Nagy2, Charles Heise2, Michelle Lyons2, Shinichi Nakai1
1

P-55

Fujifilm Corporation, 2FUJIFILM Diosynth Biotechnologies UK Limited

Development of Engineered Chicken Primordial Germ Cells for the Generation of
Antibody-Producing Transgenic Chickens
Yuya Kaneko1, Yoshinori Kawabe1, Ken-ichi Nishijima2, Masamichi Kamihira11P-56

Dept. Chem. Eng., Fac. Eng., Kyushu Univ., 2Grad. Schl. Bioagr. Sci., Nagoya Univ.

Pumpkin Seed Protein as a Gelatin Substitute for Cell-Based Meat Production
Yan Kong, Dejian Huang
National University of Singapore (Suzhou) Research Institute

P-57

High-titer rAAV Production in Bioreactors using ELEVECTA Producer Cell Lines
Kohei Natori2, J. Coronel1, A. Al-Dali1, A. Patil1, K. Srinivasan1, T. Braß1, K. Hein1, S. Wissing1
1

P-58

Cytiva, Germany, 2Cytiva (Global Life Sciences Technologies Japan), Japan

Fabrication of hepatic tissue with mesenchymal stem cells using hollow fiber
culture device
Soutarou Shimauchi1, Hiroshi Mizumoto2, Toshihisa Kajiwara2
1
2

P-59

Graduate School of Engineering, Kyushu University,
Department of Chemical Engineering, Faculty of Engineering, Kyushu University

Suppressive-effect of sericin hydrolysate on glucose consumption of mammaliancells
Naoki Shimizu1, Yuya Murakami1, Satoshi Terada1, Ryo Masugi2, Jun Takahashi2
1

P-60

University of Fukui, 2SEIREN Co. Ltd.

Scalable Production of AAV Vectors in 10 L Single-Use Orbital Shaken Bioreactors
Using Novel Human Amniotic Epithelial-Derived HAT Cell Lines
Yugo Hirai1,2, Yu-Hsin Chang1,2, Kazuko Aizawa3, Arisa Yamamoto1,2, Ryo Asahina1,2,
Rena Moromizato1,2, Michi Kubota1,2, Kazuaki Nakamura2,3, Takayuki Horiuchi1,21Chitose Laboratory Corp., 2Manufacturing Technology Association of Biologics,
3
Department of Pharmacology, National Research Institute for Child Health and Development

P-61

New Technologies And New Products For Cell Culture Media In BiopharmaceuticalIndustry Utilizing The Function Of Amino Acids
Aya M1, Fumi Shozui1, Shinya Yamaide1, Takuya Higuchi1, Ayane Sasaki1, Ayaka Sato1,
Yuki Imabayashi1, Seiichi Sato2, Masashi Harada3, Chihiro Tsuji1
1

Biopharma Media Group, Material Development Section, Material & Technology solution Labs, ResearchInstitute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc.,
2
Amino Acids Group, Biosolutions Industrialization Section, Biosolutions Labs, Research Institute for
Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc.,
3
Advanced Analysis Group, Evaluation and Analysis Section, Research Institute for Bioscience Products &Fine Chemicals, Ajinomoto Co., Inc.

27

P-62

Imaging the DNA damage response with split fluorescent protein probes
Hidenori Kaneoka1, Airi Watanabe2, Risako Fukata2, Yuki Tashiro2, Shigeki Kiyonaka2
1

P-63

Aichi Institute of Technology, 2Nagoya University

Piperine Promotes Glucose Uptake In Skeletal Muscle Via CaMK2/AMPK Pathway
Yoshito Onishi, Yoko Yamashita, Hitoshi Ashida
Kobe Univ.

P-64

Investigation of Genome Packaging-Related Factors of AAV Vectors in the Novel
Human Amniotic Epithelial-Derived HAT Cell Lines for Host Cell Development
Ryo Asahina1,2, Yugo Hirai1,2, Yu-Hsin Chang1,2, Arisa Yamamoto1,2, Rena Moromizato1,2,Michi Kubota1,2, Takayuki Horiuchi1,21P-65

Chitose Laboratory Corp., 2Manufacturing Technology Association of Biologics

Construction of production system using CHO cells for chimeric HBsAg-VLPs
presenting SARS-CoV-2 T-cell epitope
Yuki Morishita, Nam Seoyeon, Thao Bich Nguyen, Guirong Kanai, Noriko Yamano-Adachi,
Takeshi Omasa
Graduate School of Engineering, Osaka University, Japan

P-66

Analysis of metastasis suppression effects of extracellular vesicles derived from
Nanog -overexpressing melanoma cells by in vitro test using an immune cellsystem and by miRNA sequencing
Noa Katayama, Misato Nakano, Asuka Tamura, Mikako Saito
Tokyo University of Agriculture and Technology

P-67

Improvement Of The Secretory Pathway In Chinese Hamster Ovary Cells For High
Production Of Biologicals
Yurina Fujita1,2, Junta Shimada1,2, Ryo Misaki2, Hiroyuki Kajiura2, Kazuhito Fujiyama2
1
2

P-68

Graduate School of Engineering, Osaka University, Japan,
International Center for Biotechnology, Osaka University, Japan

Increased lentivirus titer using ultra expression vectors
So Fujibayashi1, Tohru Kiyono2, Yuka Endo1, Tetsuya Tani3, Haruka Tate1, Lanlan Bai1,
Eriko Sugano1, Hiroshi Tomita1, Tomokazu Fukuda1
1

P-69

Iwate University, 2National Cancer Center, 3Department of Agriculture

Hspx Is Upregulated In CHO Cells Exposed To Hyperosmolality
Mikiko Nakano1,2, Ryo Misaki2, Hiroyuki Kajiura2, Kazuhito Fujiyama2
1
2

P-70

Graduate School of Engineering, Osaka University,
International Center for Biotechnology, Osaka University

Polypropylene glycol and high molecular weight impurity detection in poloxamer 188
Damien Bellos, Atsuya Wakabayashi
Cytiva

P-71

Precision cell culture media manufacturing with trace metal and amino acidcontrolNathan Israesen, Christopher Woolstenhulme
Cytiva28

P-72

Search and Analysis of Flavonoids Upregulating Urate Transporter ABCG2
Yuki Hori1, Yasuhiro Enomoto1, Yu Toyoda3,4, Tappei Takada3, Atsuyoshi Nishina5, Hideo Satsu2
1

Maebashi Institute of Technology, 2Maebashi Inst. Technol., 3The Univ. of Tokyo Hosp.,
4
Natl. Defense Med. Col., 5Tokai Gakuen Univ.

P-73

Microarray Analysis Reveals Regulatory effects of isorhamnetin on cell cycle
and mitochondrial function of cancer-associated fibroblasts (CAFs) derived frompancreatic ductal adenocarcinoma (PDAC)
Munkhzul Ganbold1, Pakavarin Louphrasitthiphol2, Takafumi Miyamoto3,4, Yoshihiro Miyazaki2,
Tatsuya Oda2, Kenichi Tominaga1, Hiroko Isoda1,5,6
1Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), National
Institute of Advanced Science and Technology (AIST),
2
Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Faculty of Medicine, University ofTsukuba,3
Department of Internal Medicine (Endocrinology and Metabolism), Institute of Medicine, University ofTsukuba,4
Transborder Medical Research Center, University of Tsukuba,
5
Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba,
6
Faculty of Life and Environmental Sciences, University of Tsukuba

P-74

Analysis of Natural Compounds that Regulate Intestinal Epithelial Glucose
Transporter SGLT1 and GLUT5
Shimon Kimura1, Yu Satou1, Junki Yoshida3, Tadahiro Ohkuri3, Koji Nagao3, Yoshiaki Yokoo3,
Kaito Kobayashi4, Hideo Satsu2
1
2

P-75

Dept. of Biotech., Grad. Sch. of Eng., Maebashi Inst. Technol.,
Dept. of Biotech., Maebashi Inst. Technol., 3Suntory Beverage & Food Limited, 4AI Res. Ctr., AIST

Investigation of intracellular secretory process in Sar1A-overexpressed CHO cells
for further improvement of IgG productivity
Chiato Yoshiike1, Yu Tsunoda1, Noriko Yamano-Adachi1,2, Takeshi Omasa1,2
1

Graduate School of Engineering, Osaka University, Japan, 2OTRI, Osaka University, Japan

29