ȹ͎Ҕҽ‫݌‬௪࿼Ѡཅ‫ݬ‬૎ѝмуѽҨӉӜӚӣѠຉп͏ȹѝࠖыѽ૰३၏ၑѝіжјѠड़ࢂȹಚȹ ȹ ȹ হȹ ৄ̞ઃȹ ঳ȹ ‫ڲ‬ȹ პȹȹȹȹɚȹɬʍʎɽʒȹʈʇȹɞʑʉɾʋʂʆɾʇʍɺʅȹɦɾʍʁʈɽʌȹɿʈʋȹɢʇʏɾʌʍʂʀɺʍʂʇʀȹȻʍʁɾȹɟʎʇɼʍʂʈʇȹʈɿȹɠʂɻɻɾʋɾʅʅʂʇȹɽʎʋʂʇʀȹʍʁɾȹɠɾʋʆʂʇɺʍʂʈʇȹʂʇȹɩʅɺʇʍʌȹʈɿȹɩʈɺɼɾɺɾȻȹȹɢɳɮɦɢɅȹɲʈʌʁʂʁʂʋʈȹɺʇɽȹɲɚɦɚɠɮɜɡɢɅȹɤɺʓʎʌʁʂʀɾȹȹȹȹȹȹȹȹȹȹȹȹȹȹȹȹȹ൉࿾൉޸ࢯ‫گ‬࿳ળ‫޸݌‬࿨ड़ࢂࡆᅃȹ ൊ 34 ߪൊ 1 ৺2012 ໧ 4 ॆȹ သੰ
Reprinted From
THE RESEARCH BULLETIN OF THE FACULTY OFEDUCATION AND WELFARE SCIENCE,OITA UNIVERSITY
Vol. 34, No. 1̜April 2012
OITA, JAPANȹ኱ศ኱Ꮫᩍ⫱⚟♴⛉Ꮫ㒊◊✲⣖せ㸦Res. Bull. Fac. Educ.䠃Welf. Sci., Oita Univ.䠅

95ࠕ࢖ࢿ⛉᳜≀ࡢⓎⱆ᫬࡟࠾ࡅࡿࢪ࣋ࣞࣜࣥࡢാࡁࠖ࡟㛵ࡍࡿᐇ㦂᪉ἲ࡟ࡘ࠸࡚ࡢ◊✲Ἠ  ዲ ᘯ㸨࣭ ᒣ ཱྀ ୍ ⱱ㸨㸨࠙せ ᪨ࠚ  ࢖ࢿ⛉᳜≀ࡢ࣒࢜࢜ࢠ࡛ࡣ㸪✀ᏊࡀⓎⱆࡍࡿ㝿࡟⬇࡛ࢪ࣋ࣞࣜࣥࡀྜᡂࡉࢀ㸪ࢪ࣋ࣞࣜࣥࡀ⢶⢊ᒙࡢ⣽⬊ෆ࡟Ꮡᅾࡍࡿ࢔࣑࣮ࣛࢮࢆάᛶ໬ࡉࡏࡿࡇ࡜ࡀ▱ࡽࢀ࡚࠸ࡿࠋᡃࠎࡣࡇࡢ㐣⛬ࢆ⌮ゎࡍࡿࡓࡵࡢᐇ㦂࡟㛵ࡍࡿ᮲௳᳨ウࢆ⾜ࡗࡓࠋࡑࡢ⤖ᯝ㸪࣐࢝ࣛࢫ࣒ࢠࡢ୕ศษ✀Ꮚࢆᐇ㦂࡟⏝࠸ࡿࡇ࡜㸪ࢪ࣋ࣞࣜࣥࡢ⃰ᗘࡣ 100 mg / L ࡜ࡍࡿࡇ࡜㸪ᇵ㣴ᮇ㛫ࡣ 3 ᪥㛫࡜ࡍࡿࡇ࡜࡟ࡼࡗ࡚᫂░࡞⤖ᯝࡀᚓࡽࢀࡿࡇ࡜ࡀࢃ࠿ࡗࡓࠋ࣮࣮࠙࢟࣡ࢻࠚ  ࣐࢝ࣛࢫ࣒ࢠ ࢪ࣋ࣞࣜࣥ ࢔࣑࣮ࣛࢮࡢάᛶ໬Ϩ ࡣࡌࡵ࡟㧗➼Ꮫᰯ⌮⛉ࡢ⏕≀ I ࡛ࡣ㸪ࠕ⎔ቃ࡜᳜≀ࡢ཯ᛂࠖࡢ༢ඖ࡟࠾࠸࡚㸪᳜≀ࡢᡂ㛗㸪⪁໬㸪Ⓨⱆ㸪ⰼⱆᙧᡂ࡞࡝ࡀ᳜≀࣍ࣝࣔࣥ࡟ࡼࡗ࡚ㄪ⠇ࡉࢀ࡚࠸ࡿࡇ࡜ࢆᏛ⩦ࡍࡿࠋࡇࢀࡽࡢ୰࡛Ⓨⱆࡢಁ㐍࡟ࡘ࠸࡚ࡣࣞࢱࢫ࡞࡝ࡢගⓎⱆ✀Ꮚࡢ஦౛࡜࢖ࢿ⛉᳜≀ࡢ஦౛ࡀከࡃࡢᩍ⛉᭩࡟ᥖ㍕ࡉࢀ࡚࠸ࡿࠋ࢖ࢿ⛉᳜≀ࡢ࣒࢜࢜ࢠ࡛ࡣ㸪✀Ꮚࡀ྾Ỉࡍࡿ࡜⬇࡛ࢪ࣋ࣞࣜࣥࡀྜᡂࡉࢀ㸪ࡑࡢࢪ࣋ࣞࣜࣥࡀ⢶⢊ᒙࡢ⣽⬊࡟ാࡁ࠿ࡅ࡚࢔࣑࣮ࣛࢮࡀᨺฟࡉࢀࡿࡇ࡜ࡀ▱ࡽࢀ࡚࠸ࡿ  㹼  ࠋࡑࡢ࢔࣑࣮ࣛࢮ࡟ࡼࡗ࡚⬇ங୰ࡢࢹࣥࣉࣥࡀ⢾࡟ศゎࡉࢀ㸪⬇ࡣ⢾ࢆᰤ㣴ศ࡜ࡋ࡚ᡂ㛗ࡋ㸪Ⓨⱆࡍࡿࠋࡇࡢࡼ࠺࡞ࢪ࣋ࣞࣜࣥ࡟ࡼࡿ࢖ࢿ⛉᳜≀ࡢⓎⱆಁ㐍ࡣ㸪༙ษ✀Ꮚࢆ⏝࠸ࡓᐇ㦂࡛ド᫂ࡍࡿࡇ࡜ࡀ࡛ࡁ㸪ၨᯘ㤋ࡢ㧗➼Ꮫᰯ⏕≀ I ࡢᩍ⛉᭩࡛ࡣ㸪࢖ࢿࡢ✀Ꮚࢆᮦᩱ࡜ࡋࡓᐇ㦂ࡢ౛ࡀ⤂௓ࡉࢀ࡚࠸ࡿ  ࠋࡇࡢᐇ㦂࡛ࡣ㸪⬇ࢆྵࡴ༙ษ✀Ꮚ࡛ࡣ྾Ỉࡉࡏࡿ࡜ࢹࣥࣉࣥࡀศゎࡉࢀࡿࡇ࡜㸪⬇
ࢆྵࡲ࡞࠸༙ษ✀Ꮚ࡛ࡣ྾Ỉࡉࡏ࡚ࡶࢹࣥࣉࣥࡀศゎࡉࢀ࡞࠸ࡇ࡜㸪⬇ࢆྵࡲ࡞࠸༙ษ✀Ꮚ࡛ࡶࢪ࣋ࣞࣜࣥࢆ୚࠼ࡿ࡜྾Ỉ࡟ࡼࡗ࡚ࢹࣥࣉࣥࡀศゎࡉࢀࡿࡇ࡜ࡀࣚ࢘⣲ࢹࣥࣉࣥ཯ᛂ࡟ࡼࡗ࡚㸪⡆༢࠿ࡘ᫂░࡟☜ㄆ࡛ࡁࡿࡓࡵ㸪㧗➼Ꮫᰯࡢᤵᴗ࡟ྲྀࡾධࢀࡿ࡜✀ᏊⓎⱆ࡟࠾ࡅࡿࢪ࣋ࣞࣜࣥࡢാࡁࡀࡼࡃ⌮ゎ࡛ࡁࡿࡼ࠺࡟࡞ࡿ࡜⪃࠼ࡽࢀࡿࠋࡋ࠿ࡋ࡞ࡀࡽ㸪ࢪ࣋ࣞࣜࣥࡢ⃰ᗘࡸᇵ㣴ᮇ㛫࡞࡝ࡢᇵ㣴᮲௳࡟㛵ࡍࡿグ㏙ࡀ඲ࡃ↓࠸ࡓࡵ㸪ࡇࡢࡲࡲᤵᴗ࡛ᐇ㦂ࢆᐇ᪋ࡍࡿࡇ࡜ࡣ㞴ࡋ࠸࡜ᛮࢃࢀࡿࠋࡲࡓ㸪ᐇ㦂ᮦᩱ࡜ࡋ࡚ᣲࡆࡽࢀ࡚࠸ࡓ࢖ࢿࡣ୍⯡ࡢ✀ⱑᗑ࡛✀Ꮚࢆ㉎ධࡍࡿࡇ࡜ࡀ㞴ࡋ࠸ࠋࡑࡇ࡛௒ᅇࡢ◊✲࡛ࡣ㸪㧗➼Ꮫᰯ⏕≀ࡢᤵᴗ࡛ᐇ㦂ᩍᮦ࡜ࡋ࡚฼⏝࡛ࡁࡿࡼ࠺࡟ࡍࡿࡓࡵ࡟㸪㣫ᩱస≀࡛࠶ࡿ࣐࢝ࣛࢫ࣒ࢠࢆᐇ㦂ᮦᩱ࡜ࡋ࡚ᐇ㦂ࡢㅖ᮲௳ࢆ᳨ウࡋࡓࠋ
ᖹᡂ 23 ᖺ 10 ᭶ 31 ᪥ཷ⌮㸨࠸ࡎࡳ࣭ࡼࡋࡦࢁ ኱ศ኱Ꮫᩍ⫱⚟♴⛉Ꮫ㒊㸨㸨ࡸࡲࡄࡕ࣭࠿ࡎࡋࡆ ኱ศ኱Ꮫᩍ⫱⚟♴⛉Ꮫ㒊㸪኱ศᕷ❧⛵⏣ᮾ୰Ꮫᰯ

96Ἠ࣭ᒣ ཱྀϩ ᮦᩱཬࡧ᪉ἲ1 ᮦᩱ࣐࢝ࣛࢫ࣒ࢠ㸦Avena sativa L.㸧ࡢ✀Ꮚࢆᐇ㦂ᮦᩱ࡜ࡋ࡚⏝࠸ࡓࠋ࣐࢝ࣛࢫ࣒ࢠࡣ୍⯡࡟࢚ࣥࣂࢡ࡜࿧ࡤࢀ㸪๓㐍㸦୙஧✀ⱑᰴᘧ఍♫㸧࡜࠸࠺ရ✀ࡢ✀Ꮚࢆ✀ⱑᗑࡼࡾ㉎ධࡋࡓࠋ2 ᪉ἲ1) ༙ษ✀Ꮚ࠾ࡼࡧ୕ศษ✀Ꮚࡢసᡂ࣐࢝ࣛࢫ࣒ࢠࡢ✀Ꮚࢆ๋ยࡢล࡛஧➼ศࡋࡓࡶࡢ㸦௨ᚋ㸪༙ษ✀Ꮚ࡜࿧ࡪ㸧࡜୕➼ศࡋࡓࡶࡢ㸦௨ᚋ㸪୕ศษ✀Ꮚ࡜࿧ࡪ㸧ࡢ୧➃ࢆᐇ㦂࡟⏝࠸ࡓ㸦୰ኸ㒊ࡣ౑⏝ࡋ࡞࠸㸧ࠋࡲࡓ㸪⬇ࡀྵࡲࢀࡿ༙ษ✀Ꮚཬࡧ୕ศษ✀Ꮚࢆࠕ⬇᭷ࡾࠖ࡜ࡋ㸪⬇ࡀྵࡲࢀ࡞࠸᪉ࢆࠕ⬇↓ࡋࠖ࡜ࡋࡓ㸦ᅗ 1㸧ࠋ࡞࠾㸪୕ศษ✀Ꮚࡢసᡂ࡛ࡣ㸪ษ᩿ࡍࡿ㝿࡟ࠕ⬇↓ࡋࠖ࡟๋ยࡢล࠿ࡽ⬇
ࡢ⣽⬊ࡀΰධࡍࡿࡇ࡜ࢆ㜵ࡄࡓࡵ࡟㸪ྠ୍ࡢลࡣ⏝࠸࡞࠸ࡼ࠺࡟ࡋࡓࠋ2) ᇵᆅࡢ⤌ᡂ࡜ᇵ㣴᮲௳࡞࡝㸦ヲ⣽ࡣ ϫ ࡲ࡜ࡵ ࢆཧ↷㸧
0.5 %ࢹࣥࣉࣥ࡜ 1 %ᐮኳࢆྵࡴᇵᆅୖ࡟⬺࢖࢜ࣥỈࡲࡓࡣࢪ࣋ࣞࣜࣥ⁐ᾮࢆຍ࠼㸪༙ษࡲࡓࡣ୕ศษ✀Ꮚࢆ⨨ࡁ㸪ᐊ ࡛ 1 㹼 3 ᪥㛫ᇵ㣴ࡋࡓࠋ༙ษࡲࡓࡣ୕ศษ✀Ꮚ࡜⬺࢖࢜ࣥỈࡲࡓࡣࢪ࣋ࣞࣜࣥ⁐ᾮࡢ⤌ࡳྜࢃࡏࡣ㸪ࠕ⬇᭷ࡾࠖ㸩⬺࢖࢜ࣥỈ㸪ࠕ⬇↓ࡋࠖ㸩⬺࢖࢜ࣥỈ㸪ࠕ⬇↓ࡋࠖ㸩1 g / L ࢪ࣋ࣞࣜࣥ⁐ᾮ㸪ࠕ⬇↓ࡋࠖ㸩100 mg / L ࢪ࣋ࣞࣜࣥ⁐ᾮ㸪ࠕ⬇
↓ࡋࠖ㸩10 mg / L ࢪ࣋ࣞࣜࣥ⁐ᾮ㸪ࠕ⬇↓ࡋࠖ㸩1 mg / L ࢪ࣋ࣞࣜࣥ⁐ᾮࡢ 6 ✀㢮ࢆヨࡋࡓࠋᇵ㣴ᚋ㸪10 ಸ࡟ᕼ㔘ࡋࡓࣚ࢘⣲ࣚ࢘໬࣒࢝ࣜ࢘⁐ᾮࢆ㟝྿ࡁ࡛ᇵᆅ࡟྿ࡁ࠿ࡅ㸪ࣚ࢘⣲ࢹࣥࣉࣥ཯ᛂࢆ☜ㄆࡋࡓࠋ⬇᭷ࡾ⬇↓ࡋ ⬇᭷ࡾ⬇↓ࡋᅗ㸯 ࣐࢝ࣛࢫ࣒ࢠࡢ༙ษ✀Ꮚ㸦A㸧࡜୕ศษ✀Ꮚ㸦B㸧ࠋϪ ⤖ᯝ࡜⪃ᐹ᭱ึ࡟㸪༙ษ✀Ꮚࢆ⏝࠸࡚✀Ꮚࡢᇵ㣴ᮇ㛫ࢆ᳨ドࡋࡓࠋ1 ᪥㛫ᇵ㣴ࡋࡓᇵᆅ࡟ࣚ࢘⣲ࣚ࢘໬࣒࢝ࣜ࢘⁐ᾮࢆ྿ࡁ࠿ࡅࡿ࡜㸪ࣚ࢘⣲ࢹࣥࣉࣥ཯ᛂ࡟ࡼࡗ࡚ᇵᆅ඲యࡀ⃰࠸⣸Ⰽ࡟࡞ࡗࡓ㸦ᅗ
2 A㸧ࠋࡇࡢࡼ࠺࡞≧ែࡣᇵᆅࡢࢹࣥࣉࣥࡀ࡯࡜ࢇ࡝ศゎࡉࢀ࡚࠸࡞࠸ࡇ࡜ࢆ♧ࡋ࡚࠸ࡿࠋᇵ㣴

Ⓨⱆ᫬ࡢࢪ࣋ࣞࣜࣥࡢാࡁ࡟㛵ࡍࡿᐇ㦂ࡢ᮲௳᳨ウ972 ᪥ᚋ࡟ࡣ༙ษ✀Ꮚࡢ࿘㎶࡛ࣚ࢘⣲ࢹࣥࣉࣥ཯ᛂࡀᙅࡲࡾ㸦ᅗ 2 B㸧㸪ᇵ㣴 3 ᪥ᚋ࡛ࡣ༙ษ✀Ꮚࡢ࿘㎶ࡀⓑࡃᢤࡅࡓ㸦ᅗ 2 C㸧ࠋࡇࡢࡼ࠺࡞≧ែࡣᇵᆅࡢࢹࣥࣉࣥࡀศゎࡉࢀࡓࡇ࡜ࢆ♧ࡋ࡚࠸ࡿࠋࡇࢀࡽࡢ⤖ᯝࡣ㸪ᇵ㣴ᮇ㛫ࡣ 3 ᪥㛫ࡀ㐺ᙜ࡛࠶ࡿࡇ࡜ࢆ♧ࡋ࡚࠸ࡿࠋࢪ࣋ࣞࣜࣥࢆῧຍࡋࡓᇵᆅ࡛ࠕ⬇↓ࡋࠖࡢ༙ษ✀Ꮚࢆ 3 ᪥㛫ᇵ㣴ࡋࡓሙྜ࡛ࡣ㸪ࡍ࡭࡚ࡢ⃰ᗘ࡛ࢹࣥࣉࣥࡢศゎࡀ☜ㄆࡉࢀࡓ㸦ᅗ 3 C - F㸧ࠋࡇࢀࡽࡢ⤖ᯝࡣ㸪⬇ࡀ↓ࡃ࡚ࡶࢪ࣋ࣞࣜࣥࡀ࢔࣑࣮ࣛࢮࡢάᛶ໬ࢆಁ㐍ࡍࡿࡇ࡜ࢆ♧ࡋ࡚࠸ࡿࠋ1 mg / L ࢪ࡛࣋ࣞࣜࣥࡣ༙ษ✀Ꮚࡢ࿘㎶࡟ⓑࡃᢤࡅࡓ㒊ศࡀࢃࡎ࠿࡟ぢࡽࢀࡓࡔࡅࡔࡗࡓࡀ㸦ᅗ 3 C㸧㸪1 g / L ࡛ࡣⓑࡃᢤࡅࡓ㒊ศࡀ㠀ᖖ࡟኱ࡁࡃ࡞ࡗ࡚࠾ࡾ㸪ࠕ⬇᭷ࡾࠖࡢሙྜࡼࡾࡶ኱ࡁࡃ࡞ࡗ࡚࠸ࡓ㸦ᅗ 3 F㸧ࠋࢪ࣋ࣞࣜࣥࡢ⃰ᗘࡀ 10 mg / L ࡢሙྜ࡛ࡣⓑࡃᢤࡅࡓ㒊ศࡢ኱ࡁࡉࡀࠕ⬇᭷ࡾࠖࡢሙྜ࡜࡯ࡰྠࡌ࠿ᑠࡉࡃ㸦ᅗ
3 D㸧㸪10 mg / L ࡛ࡣྠ⛬ᗘ࠿኱ࡁࡃ࡞ࡗ࡚࠸ࡓ㸦ᅗ 3 E㸧ࠋࡇࡢࡼ࠺࡟㸪௒ᅇࡢᐇ㦂࡛ࡣ⤖ᯝ࡟ࣂࣛࢶ࢟ࡀ࠶ࡗࡓࡓࡵ㸪㐺ษ࡞ࢪ࣋ࣞࣜࣥࡢ⃰ᗘࢆ᏶඲࡟≉ᐃࡍࡿࡇ࡜ࡣ࡛ࡁ࡞࠿ࡗࡓࡀ㸪
10 mg / L ࡲࡓࡣ 100 mg / L ࡀᐇ㦂࡟㐺ࡋ࡚࠸ࡿࡇ࡜ࡀࢃ࠿ࡗࡓࠋࠕ⬇↓ࡋࠖࡢ༙ษ✀Ꮚࢆ⬺࢖࢜ࣥỈࡢࡳࢆῧຍࡋࡓᇵᆅ࡛ 3 ᪥㛫ᇵ㣴ࡍࡿ࡜㸪ࣚ࢘⣲ࢹࣥࣉࣥ཯ᛂ࡟ࡼࡗ࡚༙ษ✀Ꮚࡢ࿘㎶㒊ࡶ⃰࠸⣸Ⰽ࡟࡞ࡿሙྜ࡜㸦ᅗ 3 B㸧㸪༙ษ✀Ꮚࡢ࿘㎶㒊ࡀⓑࡃᢤࡅࡿሙྜࡀ࠶ࡗࡓ㸦⾲ 1㸧ࠋࡇࡢᐇ㦂ࡣ㸪⬇ࡀ↓࠸࡜ࢪ࣋ࣞࣜࣥࡀྜᡂࡉࢀ࡞࠸ࡓࡵ࡟࢔࣑
࣮ࣛࢮࡀάᛶ໬ࡋ࡞࠸ࡇ࡜ࢆ♧ࡍࡓࡵࡢࡶࡢ࡛㸪ࡍ࡭࡚ࡢሙྜ࡛๓⪅ࡢࡼ࠺࡞⤖ᯝ࡟࡞ࡿᚲせࡀ࠶ࡿࠋࡋ࠿ࡋ࡞ࡀࡽ㸪௒ᅇࡢᐇ㦂࡛ࡣ⬇ࢆᣢࡓ࡞࠸༙ษ✀Ꮚࢆࢪ࣋ࣞࣜࣥࡢᏑᅾࡋ࡞࠸ᇵᆅ
࡛ᇵ㣴ࡋࡓሙྜ࡛ࡶࢹࣥࣉࣥࡀศゎࡉࢀ࡚ࡋࡲ࠺ࡇ࡜ࡀ࠶ࡗࡓࠋࡇࡢࡼ࠺࡞⤖ᯝ࡟࡞ࡗࡓཎᅉ࡟ࡘ࠸࡚ࡣ᫂ࡽ࠿࡛ࡣ࡞࠸ࡀ㸪ᡃࠎࡣ༙ษ✀Ꮚࡢࠕ⬇↓ࡋࠖ࡟⬇ࡢ୍㒊ࡀྵࡲࢀ࡚ࡋࡲ࠺ሙྜࡀ࠶ࡿࡢ࡛ࡣ࡞࠸࠿࡜⪃࠼㸪༙ษ✀Ꮚࡢ௦ࢃࡾ࡟୕ศษ✀Ꮚࢆ⏝࠸࡚ྠᵝࡢᐇ㦂ࢆ⾜ࡗࡓࠋᅗ 4 ࡣ୕ศษ✀Ꮚࢆ⏝࠸ࡓᐇ㦂ࡢ඾ᆺⓗ࡞⤖ᯝࢆ♧ࡋࡓࡶࡢ࡛࠶ࡿࠋ༙ษ✀Ꮚࡢሙྜ࡜ྠᵝ࡟㸪⬺࢖࢜ࣥỈࡢࡳࢆῧຍࡋࡓᇵᆅ࡛ࠕ⬇᭷ࡾࠖࢆᇵ㣴ࡋࡓሙྜ࡛ࡣ㸪ࡍ࡭࡚ࡢ୕ศษ✀Ꮚࡢ࿘㎶㒊࡛ࢹࣥࣉࣥࡢศゎࡀ☜ㄆࡉࢀࡓ㸦ᅗ 4 A㸪⾲ 2㸧ࠋ୍᪉㸪⬺࢖࢜ࣥỈࡢࡳࢆῧຍࡋࡓᇵᆅ࡛ࠕ⬇↓ࡋࠖࢆᇵ㣴ࡋࡓሙྜ࡛ࡣ㸪ᇵᆅ඲య࡛ࣚ࢘⣲ࢹࣥࣉࣥ཯ᛂࡀ☜ㄆࡉࢀ㸪୕ศษ✀Ꮚࡢ
࿘㎶㒊࡟ⓑࡃᢤࡅࡓ㒊ศࡀぢࡽࢀࡿࡇ࡜ࡣࡲࡗࡓࡃ↓࠿ࡗࡓ㸦ᅗ 4 B㸪⾲ 2㸧ࠋࢪ࣋ࣞࣜࣥࢆῧຍࡋࡓᇵᆅ࡛ࠕ⬇↓ࡋࠖࢆᇵ㣴ࡋࡓሙྜ࡛ࡣ㸪ࡍ࡭࡚ࡢ୕ศษ✀Ꮚࡢ࿘㎶㒊࡛ࢹࣥࣉࣥࡢศゎࡀ☜ㄆࡉࢀࡓ㸦ᅗ 4 C - D㸪⾲ 2㸧ࠋࡇࢀࡽࡢ⤖ᯝࡣ㸪⬇ࡀ↓࠸࡜࢔࣑࣮ࣛࢮࡣάᛶ໬ࡉࢀ࡞࠸ࡀ㸪ࢪ࣋ࣞࣜࣥࢆῧຍࡍࡿ࡜⬇ࡀ↓ࡃ࡚ࡶ࢔࣑࣮ࣛࢮࡀάᛶ໬ࡉࢀࡿࡇ࡜ࢆ᫂░࡟♧ࡋ࡚࠾ࡾ㸪୕ศษ✀Ꮚࢆᐇ㦂࡟⏝࠸ࡿ࡜⌮᝿ⓗ࡞⤖ᯝࡀᚓࡽࢀࡿࡇ࡜ࡀศ࠿ࡗࡓࠋ

ᅗ㸰 ⬇ࢆྵࡴ༙ษ✀Ꮚ࡟⬺࢖࢜ࣥỈࢆῧຍࡋ࡚ᇵ㣴ࡋࡓሙྜࡢࣚ࢘⣲ࢹࣥࣉࣥ཯ᛂࠋ
A㸸ᇵ㣴㸯᪥ᚋ  B㸸ᇵ㣴㸰᪥ᚋ  C㸸ᇵ㣴㸱᪥ᚋ

98Ἠ࣭ᒣ ཱྀᅗ㸱 ༙ษ✀Ꮚࢆ㸱᪥㛫ᇵ㣴ࡋࡓᇵᆅࡢࣚ࢘⣲ࢹࣥࣉࣥ཯ᛂࠋ
A㸸ࠕ⬇᭷ࡾࠖ㸩⬺࢖࢜ࣥỈ  B㸸ࠕ⬇↓ࡋࠖ㸩⬺࢖࢜ࣥỈ
C㸸ࠕ⬇↓ࡋࠖ㸩1 mg / L ࢪ࣋ࣞࣜࣥ  D㸸ࠕ⬇↓ࡋࠖ㸩10 mg / L ࢪ࣋ࣞࣜࣥE㸸ࠕ⬇↓ࡋࠖ㸩100 mg / L ࢪ࣋ࣞࣜࣥ  F 㸸ࠕ⬇↓ࡋࠖ㸩1 g / L ࢪ࣋ࣞࣜࣥ ⾲㸯 ༙ษ✀Ꮚࢆ⏝࠸ࡓᐇ㦂ࡢ⤖ᯝᇵᆅ୰ࡢࢹࣥࣉࣥࢆศゎࡋࡓ✀Ꮚࡢᩘᇵᆅ୰ࡢࢹࣥࣉࣥࢆศゎࡋ࡞࠿ࡗࡓ✀Ꮚࡢᩘ

340ࠕ⬇↓ࡋࠖ㸩⬺࢖࢜ࣥỈ

1319ࠕ⬇↓ࡋࠖ㸩1 mg / L ࢪ࣋ࣞࣜࣥ

257

ࠕ⬇↓ࡋࠖ㸩10 mg / L ࢪ࣋ࣞࣜࣥ

320ࠕ⬇↓ࡋࠖ㸩100 mg / L ࢪ࣋ࣞࣜࣥ

320ࠕ⬇↓ࡋࠖ㸩1 g / L ࢪ࣋ࣞࣜࣥ

380

ᇵ ᆅࠕ⬇᭷ࡾࠖ㸩⬺࢖࢜ࣥỈᅗ㸲 ୕ศษ✀Ꮚࢆ㸱᪥㛫ᇵ㣴ࡋࡓᇵᆅࡢࣚ࢘⣲ࢹࣥࣉࣥ཯ᛂࠋ
A㸸ࠕ⬇᭷ࡾࠖ㸩⬺࢖࢜ࣥỈ  B㸸ࠕ⬇↓ࡋࠖ㸩⬺࢖࢜ࣥỈ
C㸸ࠕ⬇↓ࡋࠖ㸩10 mg / L ࢪ࣋ࣞࣜࣥ  D㸸ࠕ⬇↓ࡋࠖ㸩100 mg / L ࢪ࣋ࣞࣜࣥ

99Ⓨⱆ᫬ࡢࢪ࣋ࣞࣜࣥࡢാࡁ࡟㛵ࡍࡿᐇ㦂ࡢ᮲௳᳨ウ⾲㸰 ୕ศษ✀Ꮚࢆ⏝࠸ࡓᐇ㦂ࡢ⤖ᯝᇵᆅ୰ࡢࢹࣥࣉࣥࢆศゎࡋࡓ✀Ꮚࡢᩘᇵᆅ୰ࡢࢹࣥࣉࣥࢆศゎࡋ࡞࠿ࡗࡓ✀Ꮚࡢᩘࠕ⬇᭷ࡾࠖ㸩⬺࢖࢜ࣥỈ

360ࠕ⬇↓ࡋࠖ㸩⬺࢖࢜ࣥỈ

036

ࠕ⬇↓ࡋࠖ㸩10 mg / L ࢪ࣋ࣞࣜࣥ

320ࠕ⬇↓ࡋࠖ㸩100 mg / L ࢪ࣋ࣞࣜࣥ

320

ᇵ ᆅ
ϫ ࡲ࡜ࡵ௒ᅇࡢ◊✲࡟ࡼࡗ࡚㸪࣐࢝ࣛࢫ࣒ࢠ࡛ࡣ୕ศษ✀Ꮚࢆᐇ㦂࡟⏝࠸ࡿ࡜᫂░࡞ᐇ㦂⤖ᯝࡀᚓࡽࢀࡿࡇ࡜ࡀศ࠿ࡗࡓࠋࢪ࣋ࣞࣜࣥࡢ⃰ᗘ࡟ࡘ࠸࡚ࡣ⤖ᯝ࡟ࣂࣛࢶ࢟ࡀ࠶ࡗࡓࡓࡵ㸪᭱㐺࡞ࢪ࣋ࣞࣜࣥࡢ⃰ᗘࢆỴᐃࡍࡿࡇ࡜ࡣ࡛ࡁ࡞࠿ࡗࡓࡀ㸪ᑐ↷ᐇ㦂࡛࠶ࡿࠕ⬇᭷ࡾࠖ࡟ẚ࡭࡚ప࠸ຠᯝࡋ࠿ᚓࡽࢀ࡞࠸ሙྜࡀ࠶ࡿ 10 mg / L ࡼࡾࡶ㸪ࠕ⬇᭷ࡾࠖ࡟ẚ࡭࡚ࡼࡾ㧗࠸ຠᯝࡀ࡛࡚ࡋࡲ࠺ሙ
ྜࡀ࠶ࡿ 100 mg / L ࡢ࡯࠺ࡀᐇ㦂࡟㐺ࡋ࡚࠸ࡿࡢ࡛ࡣ࡞࠸࠿࡜ᡃࠎࡣ⪃࠼࡚࠸ࡿࠋ୕ศษ✀Ꮚࡢᇵ㣴ᮇ㛫ࡣ 3 ᪥㛫ࡀ㐺ษ࡛࠶ࡗࡓࡀ㸪௒ᅇࡢ◊✲࡛ࡣ㸪ᐇ㦂ࢆ 11 ᭶୰᪪࠿ࡽ 1 ᭶୰᪪࡟⾜ࡗ࡚࠸ࡿࡓࡵ㸪ᐊࡀ㧗࠸᫬ᮇ࡟ᐇ᪋ࡍࡿሙྜࡣணഛᐇ㦂ࢆ⾜ࡗ࡚㐺ษ࡞ᇵ㣴ᮇ㛫ࢆ☜ㄆࡋࡓ࡯࠺ࡀࡼ࠸࡜ᛮࢃࢀࡿࠋ࣐࢝ࣛࢫ࣒ࢠࢆ⏝࠸ࡓࠕࢪ࣋ࣞࣜࣥ࡟ࡼࡿ࢔࣑࣮ࣛࢮࡢάᛶ໬ࡢᐇ㦂ࠖ࡟ࡘ࠸࡚㸪⌮᝿ⓗ࡞⤖ᯝࡀᚓࡽࢀࡿ࡜⪃࠼ࡽࢀࡿ᪉ἲࢆ௨ୗ࡟♧ࡍࠋ1) ࣚ࢘⣲ࣚ࢘໬࣒࢝ࣜ࢘⁐ᾮࡢసᡂ㸸 ࣚ࢘⣲ࣚ࢘໬࣒࢝ࣜ࢘⁐ᾮࡣ㸪ࣚ࢘໬࣒࢝ࣜ࢘ 50 g
ࢆ⣙ 800 ml ࡢ⬺࢖࢜ࣥỈ࡟⁐࠿ࡋࡓᚋࣚ࢘⣲ 12.7 g ࢆ⁐࠿ࡋ㸪⬺࢖࢜ࣥỈ࡛ 1 L ࡟ࡋ࡚〓Ⰽ⎼࡛ಖᏑࡍࡿࠋ2) ࢪ࣋ࣞࣜࣥ⁐ᾮࡢసᡂ㸸 100 mg ࡢࢪ࣋ࣞࣜࣥ㸦GA3㸪࿴ග⣧⸆㸧ࢆ⣙ 80 ml ࡢ⬺࢖࢜ࣥỈ࡟⁐࠿ࡋ㸪1 N Ỉ㓟໬ࢼࢺ࣒ࣜ࢘⁐ᾮࢆ⁲ୗࡋ࡞ࡀࡽ᧠ᢾࡋ㸪᏶඲࡟⁐ゎࡉࡏࡓᚋ㸪⬺࢖࢜ࣥỈࢆຍ࠼࡚ 100 ml ࡟ࡍࡿ㸦ࢪ࣋ࣞࣜࣥࡢ᭱⤊⃰ᗘࡣ 1 g / L㸧ࠋࡇࢀࢆ〓Ⰽ⎼࡟ධࢀ࡚෭ⶶᗜ࡛ಖᏑࡋ㸪౑⏝┤๓࡟ 10 ಸᕼ㔘ࡋ࡚ 100 mg / L ࡟ࡍࡿࠋ3) ᐮኳᇵᆅࡢసᡂ㸸 ⬺࢖࢜ࣥỈ 100 ml ࡟ྍ⁐ᛶࢹࣥࣉࣥ 0.5 g ࡜ᐮኳ 1 g ࢆຍ࠼㸪ຍ⇕ࡋ࡞ࡀࡽ᧠ᢾࡋ࡚᏶඲࡟⁐ゎࡉࡏࡿࠋࡑࢀࢆ┤ᚄ 60 mm ࡢࣉࣛࢫࢳࢵࢡࢩ࣮ࣕࣞ࡟⣙ 5 mlࡎࡘศὀࡋ㸪ᅛࡲࡿࡲ࡛ᐊ࡛㟼⨨ࡋ㸪ୖ─࡜ୗ─ࢆࣃࣛࣇ࢕࣒࡛ࣝᅛᐃࡍࡿࠋ4) ୕ศษ✀Ꮚࡢసᡂ㸸 ๋ยࡲࡓࡣ࢝ࢵࢱ࣮ࢼ࢖ࣇࡢลࢆ⏝࠸࡚㸪࣐࢝ࣛࢫ࣒ࢠࡢ✀Ꮚࢆ㛗
㍈ࡢ 1 / 3 ࡢ఩⨨࡛ษ᩿ࡋ࡚⬇ࢆྵࡴ୕ศษ✀Ꮚ࡜⬇ࢆྵࡲ࡞࠸ࡶࡢࢆసᡂࡍࡿࠋ⬇ࢆྵࡲ࡞࠸୕ศษ✀Ꮚࢆసᡂࡍࡿሙྜࡣ㸪᪂ရࡢลࢆ⏝࠸ࡿࠋ5) ᇵ㣴࡜⤖ᯝࡢ☜ㄆ㸸 ᐮኳᇵᆅ࡟⬺࢖࢜ࣥỈࡲࡓࡣ 100 mg / L ࢪ࣋ࣞࣜࣥ⁐ᾮࢆ⣙ 1 mlຍ࠼㸪୕ศษ✀Ꮚࢆ 4 ಶ⛬ᗘ⨨ࡁ㸪ୖ─࡜ୗ─ࢆࣃࣛࣇ࢕࣒࡛ࣝᅛᐃࡍࡿࠋࠕ⬇᭷ࡾࠖ㸩⬺࢖࢜ࣥỈ㸪ࠕ⬇↓ࡋࠖ㸩⬺࢖࢜ࣥỈ㸪ࠕ⬇↓ࡋࠖ㸩100 mg / L ࢪ࣋ࣞࣜࣥ⁐ᾮࡢ 3 ✀㢮ࢆ⏝ពࡋ࡚ᐊ࡛ 3 ᪥㛫ᇵ㣴ࡋࡓᚋ㸪10 ಸ࡟ᕼ㔘ࡋࡓࣚ࢘⣲ࣚ࢘໬࣒࢝ࣜ࢘⁐ᾮࢆ㟝྿ࡁ࡛ᇵᆅ඲య࡟྿ࡁ࠿ࡅ࡚ࣚ࢘⣲ࢹࣥࣉࣥ཯ᛂࢆ☜ㄆࡍࡿࠋࡇࡢ᪉ἲ࡛ᐇ㦂ࢆ⾜࠺࡜㸪⬇ࢆྵࡴ୕ศษ✀Ꮚ࡛ࡣ྾Ỉࡉࡏࡿ࡜ࢹࣥࣉࣥࡀศゎࡉࢀࡿࡇ࡜㸪

100Ἠ࣭ᒣ ཱྀ⬇ࢆྵࡲ࡞࠸୕ศษ✀Ꮚ࡛ࡣ྾Ỉࡉࡏ࡚ࡶࢹࣥࣉࣥࡀศゎࡉࢀ࡞࠸ࡇ࡜㸪⬇ࢆྵࡲ࡞࠸୕ศษ✀Ꮚ࡛ࡶࢪ࣋ࣞࣜࣥࢆ୚࠼ࡿ࡜྾Ỉ࡟ࡼࡗ࡚ࢹࣥࣉࣥࡀศゎࡉࢀࡿࡇ࡜ࡀ᫂░࡟☜ㄆ࡛ࡁࡿࠋࡑࡢࡓࡵ㸪ࡇࡢᐇ㦂ࢆ㧗➼Ꮫᰯࡢᤵᴗ࡟ྲྀࡾධࢀࡿ࡜㸪࢖ࢿ⛉᳜≀ࡢⓎⱆࡢ㝿࡟ࡣ⬇࡛࢔࣑࣮ࣛࢮࡀྜᡂࡉࢀࡿࡢ࡛ࡣ࡞ࡃ㸪⬇࡛ࡣࢪ࣋ࣞࣜࣥࡀྜᡂࡉࢀ࡚㸪⬇௨እࡢ㡿ᇦ㸦⢶⢊ᒙ㸧࡟Ꮡᅾࡍࡿ࢔࣑࣮ࣛࢮࡢ๓㥑≀㉁ࢆࢪ࣋ࣞࣜࣥࡀάᛶ໬ࡍࡿࡇ࡜ࢆ᥎ᐹ࡛ࡁࡿࡼ࠺࡟࡞ࡾ㸪✀ᏊⓎⱆ࡟࠾ࡅࡿࢪ࣋ࣞࣜࣥࡢാࡁࡀࡼࡃ⌮ゎ࡛ࡁࡿࡼ࠺࡟࡞ࡿ࡜⪃࠼ࡽࢀࡿࠋཧ⪃ᩥ⊩(1) ᅄ᪉ ἞஬㑻 1958㸪 㯏ⱆ࡟㛵ࡍࡿ◊✲ 㸫኱㯏✀Ꮚࡢẅ⳦ཬࡧศ㞳ࡏࡿ⬇ஙࡼࡾࡢ࢔࣑࣮ࣛࢮࡢ⏕ᡂ㸫 Ⓨ㓝༠఍ㄅ 16 444  448.
(2) ᅄ᪉ ἞஬㑻 1960㸪 ࢔࣑࣮ࣛࢮ㝃ά≀㉁࡟㛵ࡍࡿ◊✲㸦➨㸰ሗ㸧 㸫⬇ᇵ㣴ᾮཬࡧ㯏ⱆᢳฟᾮ୰ࡢ࢔࣑࣮ࣛࢮ㝃ά≀㉁࡟ࡘ࠸࡚㸫 Ⓨ㓝༠఍ㄅ 18 494  499.
(3) ᅄ᪉ ἞஬㑻 1960㸪 ࢔࣑࣮ࣛࢮ㝃ά≀㉁࡟㛵ࡍࡿ◊✲㸦➨㸱ሗ㸧 㸫㯏ⱆ୰ࡢ࢔࣑࣮ࣛࢮ㝃ά≀㉁ࡢ⢭〇㸫 Ⓨ㓝༠఍ㄅ 18 500  502.
(4) ᅄ᪉ ἞஬㑻 1960㸪 ࢔࣑࣮ࣛࢮ㝃ά≀㉁࡟㛵ࡍࡿ◊✲㸦➨㸲ሗ㸧 㸫ࢪ࣋ࣞࣜࣥࡢ࢔࣑࣮ࣛ
ࢮ㝃άస⏝࡟ࡘ࠸࡚㸫 Ⓨ㓝༠఍ㄅ 18 600  602.
(5) ᅄ᪉ ἞஬㑻 1960㸪 ࢔࣑࣮ࣛࢮ㝃ά≀㉁࡟㛵ࡍࡿ◊✲㸦➨㸳ሗ㸧 㸫㯏ⱆ୰ࡢ࢔࣑࣮ࣛࢮ㝃ά≀㉁ࡢ⢭〇㸦㸰㸧ཬࡧࡑࡢᛶ㉁㸫 Ⓨ㓝༠఍ㄅ 18 603  606.
(6) ኴ⏣ḟ㑻㸪ᮏᕝ㐩㞝࡯࠿ 13 ྡ 2004㸪 㧗➼Ꮫᰯ⏕≀Ϩ ᪂⯆ฟ∧♫ၨᯘ㤋.

A Study on Experimental Methods for Investigating "theFunction of Gibberellin during the Germination in Plants ofPoaceae"
IZUMI, Yoshihiro and YAMAGUCHI, Kazushige

Abstract
In Hordeum vulgare of Poaceae,  it is known that gibberellin issynthesized in an embryo during the seed germination and activates
amylase in aleurone cells.

We carried out a study of the requirements

for experiments to understand with the this process.

This study showedthat the experiment is successful with the culture of one-third cut seeds ofAvena sativa without an embryo on a medium containing 100 mg / L
gibberellin.
䛆Key wordsࠚ  Avena sativa,

Gibberellin,

Activation of amylase