HTS Customer Care
Kits
User’s Guide

For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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23-11165-00 Rev. A
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FCC Information
WARNING: Changes or modifications to this unit not expressly approved by the party responsible for compliancecould void the user’s authority to operate the equipment.
NOTICE: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant
to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference
when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmfulinterference to radio communications. Operation of this equipment in a residential area is likely to cause harmfulinterference in which case the user will be required to correct the interference at his or her own expense.
Shielded cables must be used with this unit to ensure compliance with the Class A FCC limits.
This Class A digital apparatus meets all requirements of the Canadian Interference-Causing Equipment Regulations.
Cet appareil numérique de la classe A respecte toutes les exigences du Réglement sur le matériel brouilleur duCanada.

Compliance Information
NOTICE: This laboratory equipment has been tested and found to comply with the EMC and the Low Voltage
Directives. This includes FCC, Part 15 compliance for a Class A Digital Device.
CAUTION: Any unauthorized modifications to this laboratory equipment may affect the Regulatory Compliance
items stated above.

History
Revision

Date

Change Made

23-11165-00 Rev. A

1/2010Initial release

Contents
Chapter 1: Overview of HTS Customer Care1

About the HTS Customer Care Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Kit Configurations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
When to Use the Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
About the HTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
HTS Configurations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Location of Commonly Clogged Components . . . . . . . . . . . . . . . . . . . . . . . . 5
Safety and General Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Biological Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Chapter 2: HTS Removal and Reinstallation

9

BD FACSCanto II System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Removing the HTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Reinstalling the HTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
BD FACSCanto, BD LSR II, BD FACSCalibur, and BD LSRFortessa Systems . . 12
Removing the HTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Reinstalling the HTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Chapter 3: Wash Station Assembly Replacement

17

Required Kit Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Replacing Wash Station Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Assembling the Sample Injection Tubing Assembly . . . . . . . . . . . . . . . . . . . 20v

Chapter 4: Tubing Assembly Replacement

23

Required Kit Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Replacing Tubing Assembly Components . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Chapter 5: Sample Coupler and Tubing Replacement27

BD FACSCanto and FACSCanto II Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
BD FACSCalibur, BD LSR II, or BD LSRFortessa Systems . . . . . . . . . . . . . . . . . 30
Chapter 6: Pump Syringe Replacement

31

Required Kit Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Pump Syringe Replacement Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Removing Liquid from the Syringe (For Primary Pump Syringe) . . . . 32
Replacing the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Chapter 7: Fluidic Connection and In-line Fluidic Filter Replacement

37

Required Kit Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Replacing the Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Chapter 8: Probe and Probe Tubing/Body Assembly Replacement

41

Required Kit Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Replacing the Probe and Probe Tubing/Body Assembly . . . . . . . . . . . . . . . . 42
Chapter 9: Cleaning/Shutdown Procedure

45

Required Kit Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Cleaning Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45vi

HTS Customer Care Kits User’s Guide

1
Overview of HTS Customer Care
About the HTS Customer Care Kits
The HTS Customer Care kits can help keep the BD High Throughput Sampler
Option (HTS) in peak operating condition. The HTS will experience problems if
sheath fluid crystallization occurs in the connectors, tubing, and syringes.
Replacing these components every six months or annually, along with dailycleaning and fluidics cleaning and shutdown, can prevent clogs and optimize
system performance. The HTS Customer Care Kit offers a convenient way toreplace clogged connectors, tubing, and syringes on site. The kit, which contains
customer-replaceable components and instructions, reduces downtime and theneed for depot repair.

1Kit Configurations
The HTS Customer Care Kit is designed to custom fit the HTS configuration foreach cytometer or group of cytometers. Therefore, order the correct kit number
by using the following table.
Part number

Description

Cytometer

644787HTS Kit Parts Replacement

BD FACSCanto™
BD FACSCanto™ II

644788HTS Kit Parts Replacement

BD FACSCalibur™
BD™ LSR II (including special order
BD LSR II)
Special order BD LSRFortessa™

When to Use the Kit
The parts in the HTS Customer Care Kit can be used to resolve common
problems. The following table is lists of symptoms, possible causes, and solutions
that are described in this user’s guide.

2

Symptom

Possible Causes

Solution

See...

High carryover

Wash station connections
are worn

Replace wash station
components

Chapter 3

Secondarypump (Pump-2)
error

Clog in sample coupler
tubing, or in pumpsyringe, or syringe needs
replacement

Replace sample coupler
tubing or pumpsyringes

Chapter 5

Primary pump
(Pump-1) error

Clog in probe, or tubing
assembly from probe to
the primary pump, or inpump syringe, or syringeneeds replacement

Replace probe, tubing
assembly components,
or pump syringes

Chapter 4

HTS Customer Care Kits User’s Guide

Chapter 6

Chapter 6

SymptomPossible Causes

Solution

See...

Wash station
overflows

Wash station clog

Replace the wash
station assembly

Chapter 3

No events
detected

Clog in sample coupler
tubing or tubing
assembly

Replace tubing
assembly components
or sample coupler
tubingChapter 5

Leak in sample
coupler

Loose coupler or clog in
coupler tubing

Tighten coupler (seeprecautions) or replacesample coupler tubing
when necessary

Chapter 5

Reduced sheathflow

Sheath fluid filter is dirty
or clogged

Replace fluidic filter
every six months orwhen clogged

Chapter 7

Probe does nottake up sample

Probe might be bent or
cloggedReplace probe tubing
assembly

Chapter 8

About the HTS
Illustrations of the HTS and a schematic of the fluidics system are provided forreference. For more detailed information, see your BD High Throughput Sampler
User’s Guide.

HTS Configurations
The following figures show the front and rear views of the HTS for eachcytometer.

Chapter 1: Overview of HTS Customer Care3

Figure 1-1 HTS for the BD FACSCanto II system

Figure 1-2 HTS for the BD FACSCalibur and BD FACSCanto systems

Note: The module
body color for the
BD FACSCanto isblue

4

HTS Customer Care Kits User’s Guide

Figure 1-3 HTS for the BD LSR II, special order BD LSR II and BD LSRFortessa systems

Note: The
module bodycolor might beunique to yoursystem.

NOTE The main HTS module shown is the same for all BD LSR products. The
BD LSRFortessa analyzer has an extra protective cover that mounts to theoriginal BD LSR II HTS cover.

Location of Commonly Clogged Components
The clogs that occur and need repair are in the fluidics system. The following
schematic identifies the most commonly clogged components. The numbers refer
to component parts in the Required Kit Components tables throughout thisguide.

Chapter 1: Overview of HTS Customer Care5

Figure 1-4 Schematic of the HTS fluidics system

4
PressureswitchFlow cell
Waste
pump

SIT

Primary
pump

12
Probe

Wash station
1

Tray

6

2
3
Valve

5a

6
HTS
pump

6

HTS Customer Care Kits User’s GuideSafety and General Precautions
Biological Safety
All instrument surfaces that come in contact with biological specimens
can transmit potentially fatal disease.
Use universal safety precautions when changing tubing or removing any
part in contact with the fluidics system.
Wear suitable protective clothing, eyewear, and gloves.

Electrical Safety
Ensure that the power to the HTS is turned off before handlingelectrical cables and their connections.

Precautions
1

Tighten all fittings finger tight. Do not use tools to further tighten
connectors.

2

If a connector is difficult to remove because of saline deposits, clean with
deionized (DI) water, then carefully use the 5/16 wrench in the accessory kit
to remove the connector.

3

The sealing washers in the valves of each port can be compressed, and will
eventually block the port. To avoid compression, tighten fittings until they
make contact with the sealing washer and then turn the fitting an
additional 1/6 to 1/4 turn. If there is a leak, tighten the fitting no more thanan additional 1/8 turn.

Chapter 1: Overview of HTS Customer Care

7

THIS PAGE INTENTIONALLY LEFT BLANK2HTS Removal and Reinstallation
Before performing replacement procedures, remove the HTS from the cytometer.
Pay particular attention to the following:•

Always disconnect electrical connections (interlock cable and interface
cable) and fluidic connections (waste, sheath, and sample injection tubing(SIT)).

•

The waste and sheath fittings on the back side of the HTS can easily be
broken when moving the HTS away from the cytometer while these fittings
are connected. Move the HTS just enough (approximately 2 inches) toprovide the room necessary to disconnect the waste and sheath fittingsfrom the HTS.
All instrument surfaces that come in contact with biological specimens
can transmit potentially fatal disease.

BD FACSCanto II System
Removing the HTS
1Turn off the cytometer and HTS.

2

Remove or open the BD High Throughput Sampler safety cover.

Chapter 2: HTS Removal and Reinstallation

9

3

10

Detach the sample coupler from the cytometer SIT.
a

Unscrew the top nut.

b

Pull the sample coupler down and away from the SIT, leaving the nut
attached to the sample coupler.

4

Unscrew the captive screw located at the bottom of the HTS support
bracket.

5

Pull the HTS out (about 2 inches) from the support bracket.

6

Remove the probe and the overflow reservoir.

7

Disconnect interlock and interface cables.

8

Disconnect the sheath and waste connectors from the HTS.

9

Disconnect the sampler coupler from the cytometer SIT.

10

Disconnect the door sensor cable.
aLift the front feet of the HTS just over the front edge of the enclosureand tilt the unit at a 45° angle toward you for access to the door sensor
cable on the right side.

b

Unplug the door sensor cable by pulling back on the ferrule.

c

Place the cable over the right side of the enclosure/carrier.

HTS Customer Care Kits User’s Guide

11

Place the HTS unit on a stable work bench.

Sheath tubing
connection
Waste tubing
connection

Interlock cableconnection
Interface cableconnection

Sampler coupler

Reinstalling the HTS
1Place HTS on support bracket.

2

Connect the interlock cable to the HTS.

3

Connect the interface cable to the HTS.

4

Connect the waste tubing to the waste connector on the HTS.

5

Connect the sheath tubing to the sheath connector on the HTS.

6

Connect the sampler coupler to the SIT on the cytometer.

Chapter 2: HTS Removal and Reinstallation

11

7

Tighten the captive screw that secures the HTS to the support bracket.

8Turn on the cytometer.

BD FACSCanto, BD LSR II, BD FACSCalibur, and BD
LSRFortessa Systems
Removing the HTS
1Turn off the cytometer and HTS.

2

Remove the HTS cover.

3

Unscrew the captive screw located at the bottom of the HTS support
bracket.
NOTE There is no captive screw in BD LSRFortessa, because the HTS isplaced on a sliding drawer.

4

Pull the HTS out (about 2 inches) from the support bracket.

5

Remove the probe and the overflow reservoir.

6

Disconnect the power cable and the serial communications cable.

7

Disconnect the sampler coupler from the cytometer SIT.

8

Disconnect the sheath and waste connectors from the cytometer.

9

Remove the HTS from the support bracket.
a

10

12

Support the HTS on its base, raise the back of the HTS to balance theload, and lift it off the support bracket.

Place the unit on a stable work beach and replace the necessary part.

HTS Customer Care Kits User’s Guide

Captive screw
HTS positioned on its support bracket

Chapter 2: HTS Removal and Reinstallation

13

Figure 2-1 Electrical connection to the HTSPower plug

Serial cable

14

HTS Customer Care Kits User’s Guide

Figure 2-2 Fluidic connections to HTS

Routing of power andcommunication cables

Waste fluidic connection

Sheath fluid connection

Reinstalling the HTS
1Place the HTS on the support bracket.

2

Reconnect the power cable and the serial communications cable.

3

Reconnect the sheath and waste connectors to the HTS.

Chapter 2: HTS Removal and Reinstallation

15

16

4

Slide the HTS unit as far as it can go.

5

Secure the mounting screw to the bottom on the HTS.

6

Place the cover back on the HTS and turn it on.

7

Attach the sample coupler to the SIT.

8

Turn on the cytometer.

9Start BD FACSDiva™ software or Plate manager software if connected to
the BD FACS Calibur and perform an HTS prime.

HTS Customer Care Kits User’s Guide

3
Wash Station Assembly
Replacement
Perform the wash station assembly maintenance tasks every six months or when
the following symptoms occur:•

The HTS exhibits higher than normal carryover between samples•

The wash station overflows

Required Kit Components
The sample injection tubing (3) connects the injection port/wash station (1) to thesecondary pump valve (not shown).
ItemnumberPart
NumberDescription

Qty

1

336947

Injection port/wash station

1

2335526

Tubing, injection port

1

3335451Tubing assembly, sample injection tubing to
HTS pump

1

Chapter 3: Wash Station Assembly Replacement

17

Sampler coupler

Sealing washer

Injection port tubing
Injection port/wash station

Replacing Wash Station Components

181

Perform the monthly cleaning procedure to decontaminate the sample
injection port/wash station. For instructions, see your BD HighThroughput Sampler User’s Guide.

2

Turn off the cytometer and HTS if needed.

3

Remove the HTS according to procedure in Chapter 2.

4

Move the probe arm up and toward the front of the HTS.

5

Unscrew and remove the sample injection tubing from the top of thesecondary pump.

HTS Customer Care Kits User’s Guide

Tubing assemblySecondary pump

The tubing nuts are normally finger tight. If it is difficult to unscrew atubing nut, use a 5/16 wrench to loosen it.
6

Turn the sample injection port (see figure) slightly to disengage themagnets, and lift the port approximately 1 inch.

7

Disconnect the silicon tubing from the sample injection port. Do notdisconnect the silicon tubing at the lower end because this could damage
the lower connector.

.

SilicontubingChapter 3: Wash Station Assembly Replacement

198Secure the silicon tubing so that it does not slide back into the hole, andcarefully remove the sample injection port assembly.

Assembling the Sample Injection Tubing Assembly
To prevent the cross-threading of fittings, ensure that mating fittings arealigned to one another before screwing them together. All fittings should besecured only finger tight and not beyond.

20

1Connect the sample injection port to the sample injection port tubing
assembly.

2

Connect the injection port tubing assembly to the sample injection tubing
assembly.

3

Lower the sample injection port tubing assembly into the port hole andconnect the silicon tubing to the sample injection port base (as shown in thefollowing figure). The magnets will secure the sample injection port inplace.

HTS Customer Care Kits User’s Guide

4

Install and tighten the sample injection tubing assembly to the top of thesecondary pump and install the HTS on the cytometer (see Chapter 2).

Chapter 3: Wash Station Assembly Replacement

21

THIS PAGE INTENTIONALLY LEFT BLANK

4
Tubing Assembly Replacement
Perform the tubing assembly from probe to the primary pump maintenance tasks
when the following symptoms occur:•

Tubing is clogged•

The systems displays a primary pump (Pump-1) error•

No events are detected

Required Kit Components

ItemnumberPart Number

4335454

Description

Qty

Tubing assembly, sample probe to the1

primary pump

Chapter 4: Tubing Assembly Replacement

23ClampTubing assemblyPrimary pump

NOTE Use caution when handling the sample injection port assembly. This
assembly is fragile and can be easily damaged.

Replacing Tubing Assembly Components
1

Turn off the cytometer and HTS and move the sample probe into thesample injection port.

2

Remove the sample probe tubing assembly.

3

Unscrew the sample injection tubing from the probe and the top of primarypump.
The sample injection tubing connectors are indicated in the following
figure.

24

HTS Customer Care Kits User’s Guide

4

Remove the sample probe tubing by rotating the tubing clamp on the arm
to allow enough clearance for the tubing to be removed. If a connector isdifficult to remove due to saline deposits, clean it with DI water. If it still
does not unscrew, then carefully use the 5/16 wrench and gently loosen theclamp. Do not force.

5

Connect the sample probe tubing.
NOTE Valves contain sealing washers in each port. If you over-tighten thefitting, the sealing washers can be compressed, resulting in a blocked port.
Tighten the fitting until it contacts the sealing washer, and then turn thefitting an additional 1/6 to 1/4 turn. If leaking is observed, tighten thefitting no more than an additional 1/8 turn.

6

Install the sample probe tubing in the clamp on the arm and rotate theclamp back into position.

7

Install and tighten the sample injection tubing to the sample probe and to
the top of the primary pump.

8

Turn on the cytometer and perform a Prime.

Chapter 4: Tubing Assembly Replacement

25

THIS PAGE INTENTIONALLY LEFT BLANK

5
Sample Coupler and Tubing
Replacement
The sample coupler is frequently installed or removed from the SIT to run thecytometer in tube acquisition mode. The sample coupler tubing is attached to the
HTS secondary pump.
Replace the sample coupler tubing annually or when the following symptoms
occur:•The system displays a secondary pump (Pump-2) error•

The sample coupler continues to leak after being tightened

A rare symptom can be no events are detected.

Chapter 5: Sample Coupler and Tubing Replacement27

BD FACSCanto and FACSCanto II Systems
Item number

Part number

Description

Qty

5

339340Sample coupler, secondary pump to SIT

1Figure 5-1 Sample coupler and tubing for the BD FACSCanto and BD FACSCanto II systems

Sample coupler, secondary pump to SIT

Secondary pump28

1Turn off the cytometer, and remove the HTS from the cytometer (seeChapter 2).

2

Unscrew the sample coupler tubing from the left port of secondary pumpvalve (indicated in the figure) and discard the sample coupler with tubing
into a biohazardous waste container.

HTS Customer Care Kits User’s Guide

Secondary pump valve

3

Install the new sample coupler tubing provided in the HTS Customer Care
Kit.

4

Connect the new tubing to the left port in the secondary pump valve, andtighten the tubing until the fitting is finger tight.
NOTE Valves contain sealing washers in each port. If you over-tighten thefitting, the sealing washers can be compressed, resulting in a blocked port.
Tighten the fitting until it contacts the sealing washer, and then turn thefitting an additional 1/6 to 1/4 turn. If leaking is observed, tighten thefitting no more than an additional 1/8 turn.

5

Install the HTS on the cytometer (see Chapter 2).

Chapter 5: Sample Coupler and Tubing Replacement29

BD FACSCalibur, BD LSR II, or BD LSRFortessa
Systems
Item number

Part number

Description

Qty

5a

335452

Sample coupler, secondary pump to SIT

1

Sample coupler, secondary
pump to SIT

Secondary pump30

1Fill a small, clean container with approximately 300 mL of DI water.

2

Remove the sheath line from the HTS.

3

Connect the purge tubing to HTS sheath port (see Chapter 9).

HTS Customer Care Kits User’s Guide

6
Pump Syringe Replacement
Glass syringes are used in the primary and secondary pumps of the HTS unit. For
reference, the syringe associated with the secondary pump is shown the following
figure.
Sharp raised edge
Syringe
Narrow up

Syringe barrel

1/4 - 28 thread

Perform the replacement of the pump syringe maintenance tasks annually or
when the following symptoms occur:•

Primary or secondary pump (Pump-1 or Pump-2) errors occur•

A Pump syringe has a clog

NOTE It is not necessary to remove the HTS when replacing the primary pumpsyringe (located on the front of the HTS). However, it is necessary to remove the
HTS when replacing the secondary pump syringe (located on the rear of the
HTS).

Chapter 6: Pump Syringe Replacement31

Required Kit Components
Item number

Part number

Description

Qty

6344912Syringe, 500 µL, with black plunger seal

1

7347306

Lubricant, O-ring, Super O-Lube, ½ounce tube

1

Pump Syringe Replacement Procedures•

If you are replacing the primary pump, use both procedures in this chapter.

•If you are replacing the secondary pump, remove the HTS (see Chapter 2),
then use the Replacing the Syringe procedures.

Removing Liquid from the Syringe (For Primary Pump Syringe)
1

Verify that the cytometer and BD FACSDiva software are running.

2

From the Acquisition Dashboard select RUN PLATE.

3

Remove the liquid from the syringe:

4

32

a

Disconnect the sheath connector from the back of the HTS.

b

Disconnect one end of the sheath filter.

c

Select HTS > Prime.

dRepeat until the syringe barrel is empty.

eExit BD FACSDiva software.

Turn off the cytometer.

HTS Customer Care Kits User’s GuideReplacing the Syringe1

Loosen the plunger lock screw (indicated in the following figure)approximately three full turns.

Valve
Syringe barrel

Plunger lock screw

2

Lower the plunger drive by pushing the plunger lock screw down.

3

Unscrew the syringe barrel and remove it from the lower part of the valve.
Grasp the metal ring at the top of the syringe and turn counterclockwise.

4

Discard the used syringe into a biohazard sharps container.

5Install the new syringe:a

Screw the syringe barrel onto the valve until it contacts the seal washer,then turn the syringe an additional 1/6 to 1/4 turn. If you observe a
leak, tighten a maximum of an additional 1/8 turn.

b

Pull the syringe plunger down to the plunger holder (see the following
figure).

Chapter 6: Pump Syringe Replacement33

cTighten the plunger lock screw, making sure it is securely tightened.

Plunger
Plungerholder34

Lubricant6

Apply a generous amount of O-ring lubricant to the plunger lock screw toprevent seizing in the future. You can apply the lubricant in the lock leverhole before placing the plunger in it. This will help keep the saline out of
the whole assembly Do not lubricate the syringe barrel.

7

For the secondary syringe, reinstall the HTS on the cytometer (seeChapter 2).

HTS Customer Care Kits User’s Guide

8

Turn on the cytometer and using the BD FACSDiva software, perform a
Prime.

Secondary pump

Syringe

Chapter 6: Pump Syringe Replacement

35

THIS PAGE INTENTIONALLY LEFT BLANK

7
Fluidic Connection and In-line
Fluidic Filter Replacement
This chapter covers the replacement of the two fluidic connections between thecytometer and the HTS. These two fluidic connections are sheath and waste
connections.
Perform the fluidic connections maintenance and replace the sheath filter:•

Every six months•

When the sheath flow is reduced or inadequate

Chapter 7: Fluidic Connection and In-line Fluidic Filter Replacement37

Required Kit Components
Item number

Part number

Description

Qty

8335710Filter, 25 mm, 17-µm screen

1

9343527

Coupling insert, hose barb (blue)110

59-10090-05

Coupling insert, hose barb (orange)(waste)1

11343529

Coupling insert, hose barb (white)

1

Figure 7-1 Filter

FilterFigure 7-2 BD FACSCanto II connectors

Blue hose barb
Orange hose barb
FilterWhite hose barb

38

HTS Customer Care Kits User’s GuideReplacing the Filters
1Turn off and remove the HTS from the cytometer (see Chapter 2).

2

Replace the two liquid connections between the cytometer and the HTS:a

Move the HTS away from the cytometer to provide access behind the
HTS.

bDisconnect the quick-connector at the cytometer interface panel andthe back of the HTS (orange quick-connector end in Canto II).

c

Disconnect the quick-connector at the cytometer interface panel and at
the white quick-connector on the back of the HTS unit (blue quickconnector end in Canto II).

d

Remove the tubing from the filter by twisting the Luer connections.

eInstall the sheath line with the lock-ring end toward the cytometer
panel.

.

This will prevent foreign material that accumulates on one side of thefilter from flushing into the HTS if the filter is reversed.
3

Replace the filter and reconnect all tubing.

4

Install the HTS on the cytometer (see Chapter 2).

Chapter 7: Fluidic Connection and In-line Fluidic Filter Replacement

39

THIS PAGE INTENTIONALLY LEFT BLANK

8Probe and Probe Tubing/Body
Assembly Replacement
The probe is located at the end of the probe assembly arm.
Replace the probe along with the probe tubing or body assembly annually, or
when the following occurs:•

Probe is bent or is not taking up sample•

Probe is clogged

Required Kit Components
Item number

Part number

Description

Qty

12

34389017

Probe tubing/body assembly

1

NOTE

A spare probe is included in the HTS accessory kit.

Chapter 8: Probe and Probe Tubing/Body Assembly Replacement41

Replacing the Probe and Probe Tubing/Body
Assembly
The sample injection port is fragile and can be easily damaged. Use carewhen handling the sample injection port.
1Turn off the cytometer and remove the HTS cover.
NOTE On the BD FACSCanto, BD LSR II, and BD FACS Calibur systems,
you will need to turn off the power on the cytometer and the HTS
separately.

2

Pull the HTS arm up slowly and move it toward the front of the
instrument.

Probetubing/bodyassembly42

HTS Customer Care Kits User’s Guide

3

Unscrew the tubing connector from the probe.

Tubing connector
Probe nut
Probe

4

Unscrew the probe nut and remove the probe from the arm.
Probe
Probe nut
Tubing connector

5

Install and tighten a new probe.
aTighten the probe nut at the top of the probe and attach the tubing
connector to the probe.

6

Turn on the cytometer and HTS if needed.

7

Start BD FACSDiva software and perform an HTS prime.

Chapter 8: Probe and Probe Tubing/Body Assembly Replacement

43

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9Cleaning/Shutdown Procedure
The BD FACSCalibur, BD LSR II, and BD LSRFortessa do not have a Fluidic
Shutdown cycle that automatically replaces BD FACS™ sheath solution with BD
FACS shutdown solution or DI water. These procedures are for the BD
FACSCalibur, BD LSR II, Special Order BD LSR II, and Special Order BD
LSRFortessa systems as they do not have a fluidic shutdown cycle built into thesoftware.
We recommend daily cleaning and maintenance of the sample coupler and tubing
to avoid clogs.

Required Kit Components
Item number

Part number

Description

Qty

13

340088

HTS purge tubing

1

Cleaning Procedures
1

Fill a clean container with approximately 300 mL of deionized (DI) water.

2

Remove the sheath line from the HTS.

3

Connect the purge tubing to the HTS sheath port.

Chapter 9: Cleaning/Shutdown Procedure

45

HTS purge tubing

BD HTS sheath port

Purge tubing

DI water

4

Submerge the other end of the purge line into the DI water.

5

Using BD FACSDiva software, perform a system prime. Repeat 3 times.
For BD FACS Calibur, use BD Plate Manager software to run the prime.

46

6

Remove the purge tubing.

7

Reinstall the sheath line.

HTS Customer Care Kits User’s Guide