Chromatin Immunoprecipitation

NEW: Ready-to-use ChIP Columns and Fixation Buffer for Your ChIP Protocols
For labs that routinely perform ChIP, Active Motif is making it faster and easier to obtain consistency in chromatin
preparations and improve the quality of the ChIP reactions. The addition of ChIP-IT® Fixation Buffer during chromatin extraction improves both the consistency of sample preparation and also enhances ChIP efﬁciency. With Active
Motif’s ready-to-use Protein G Agarose Columns, simply add the ChIP reaction directly into the column to perform
incubation and washing steps. The columns provide a faster alternative to centrifugation methods and ensurecomplete sample recovery for better reproducibility. For more information, visit www.activemotif.com/protein-g.
Ready-to-use ChIP Columns
Active Motif’s new Protein G Agarose
Columns provide the perfect solution
for labs that routinely perform ChIP. The
columns contain high-afﬁnity protein
G agarose beads that strongly bind IgG.
The beads have been speciﬁcally engineered to eliminate non-speciﬁc binding,making them a better option than traditional agarose or protein G magnetic
beads. The protein G agarose supplied ineach column has a binding capacity of
10 µg IgG/µl bead, and can be adapted
to work with any ChIP protocol.
The beads have been pre-washed andloaded into ﬁltration columns. Simply
add the ChIP reaction to the columns
and use the cap to seal. The incubation
and wash steps are all performed within
the column to streamline the process
and prevent the loss of any material.
This provides a faster solution tocentrifugation and magnetic separation
methods, and results in better reproducibility between samples because nomaterial is lost.
In addition to ChIP, the Protein G
Agarose Columns can also be used for
co-immunoprecipitation (Co-IP) experiments. The columns have been validated
using Active Motif’s Nuclear Complex
Co-IP Kit (Catalog No. 54001), where
the strong binding afﬁnity and reduced
background of the protein G agarose
make these columns an ideal tool for
antibody capture during Co-IP.

8Figure 1: Image of a Protein G Agarose Column.
ChIP reactions can be added directly to the Protein G Agarose Column andincubations are performed directly in the column. To wash, simply snap off
the tab at the bottom and place the column in an empty 1 ml pipet tip box
for use as a holder to perform the wash steps.

ChIP-IT Fixation Buffer
The unique properties of the ChIP-IT
Fixation Buffer provide consistency between chromatin preparations for better
reproducibility across experiments. This
specially formulated buffer is combinedwith formaldehyde and added directly
to the cell culture growth medium (withor without serum) or to tissue material.
The buffer helps eliminate pH effects
during chromatin preparation for moreconsistent ChIP results.

Protein G Column advantages• Ready-to-use – the beads havebeen pre-washed and loaded
into the column• No pre-blocking needed – theprotein G agarose beads havebeen engineered to eliminate
non-speciﬁc binding• Avoid sample loss – the column
ensures complete retention of
the beads to prevent sample loss
• Versatility – use them for both
ChIP and Co-IP experiments

ProductFormat

Catalog No.

Protein G Agarose Columns

30 rxns
5 rxns

53039
53037

ChIP-IT® Fixation Buffer

3 ml

53038

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