Aqua TROLL 600

Operator’s Manual

Information subject to change without notice. In-Situ, In-Situ logo, Baro Merge, BaroTROLL, HERMIT, HydroVu™, iSitu,
Pocket-Situ, RDO, RuggedCable, RuggedReader, SmarTROLL™, TROLL, VuSitu, and Win-Situ are trademarks or registeredtrademarks of In-Situ Inc.©2016. All rights reserved. This product may be covered by patents identified at www.in-situ.com/
patents

2022-07-13 | 0096402

Contents
Introduction.......................................................................................................................................................................................... 6
Serial Number Location................................................................................................................................................................ 6
Instrument Description................................................................................................................................................................. 6
Document Conventions............................................................................................................................................................... 6
Unpacking and Inspecting.......................................................................................................................................................... 6
Obtaining Repair Service.............................................................................................................................................................. 7
Guidelines for Cleaning Returned Equipment..................................................................................................................... 8
Safety...................................................................................................................................................................................................... 9
Intended Use.................................................................................................................................................................................... 9
Instrument Specifications..............................................................................................................................................................10
External Power Source Requirements...................................................................................................................................11
Sensor Specifications......................................................................................................................................................................13
Sensor Summary...........................................................................................................................................................................13
Solutions..........................................................................................................................................................................................14
Potential Interferents...................................................................................................................................................................15
RDO Cap Chemical Incompatibility........................................................................................................................................15
Conductivity/Temperature Sensor ........................................................................................................................................15
Ammonium, Chloride, and Nitrate Interferent Concentrations...................................................................................16
Required Accessories......................................................................................................................................................................25
Communication Device..............................................................................................................................................................25
Cable..................................................................................................................................................................................................25
Sensors..............................................................................................................................................................................................25
Wireless TROLL Com.....................................................................................................................................................................25
Rugged Twist-Lock Cable...........................................................................................................................................................25
Software...........................................................................................................................................................................................26
Telemetry.........................................................................................................................................................................................26
System Components.......................................................................................................................................................................27
Base Unit Components...............................................................................................................................................................27

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Getting Started..................................................................................................................................................................................31
Handling pH and Ion-Selective Electrode Sensors (ISEs)...................................................................................................34
Using the RDO Sensor and RDO Fast Cap................................................................................................................................34
Ion-Selective Electrode Sensor (ISE) Requirements.............................................................................................................35
Ammonium Sensor Requirements.........................................................................................................................................35
Chloride, Nitrate, and pH/ORP Sensor Requirements......................................................................................................35
LCD Screen..........................................................................................................................................................................................36
Accessing the LCD Menu............................................................................................................................................................36
Possible Power Statuses.............................................................................................................................................................36
Possible Connected Statuses....................................................................................................................................................36
Possible Port Statuses..................................................................................................................................................................36
Possible Log Statuses..................................................................................................................................................................36
Error Messages...............................................................................................................................................................................37
Starting a Log with the LCD Menu..........................................................................................................................................37
Stopping a Log...............................................................................................................................................................................37
Stopping a Log ..............................................................................................................................................................................38
Adjusting the LCD Menu Contrast..........................................................................................................................................38
Setting or Changing Menu Language...................................................................................................................................39
Viewing the Updates Menu.......................................................................................................................................................39
Micro SD Card.................................................................................................................................................................................40
Replacing Desiccant........................................................................................................................................................................42
Calibrating Sensors..........................................................................................................................................................................42
Solution-Based Calibration........................................................................................................................................................42
RDO 100% Saturation Calibration: Water Saturated Air..................................................................................................43
RDO 100% Saturation Calibration: Saturation Bubbler...................................................................................................43
RDO Salinity Setting.....................................................................................................................................................................44
Turbidity Sensor Calibration.....................................................................................................................................................44
Batch Calibrating Sensors in VuSitu...........................................................................................................................................45
Different Sensor Types (Quick Cal Solution)........................................................................................................................45
Multiple Sensors of the Same Type........................................................................................................................................46
Zeroing the Depth Sensor.........................................................................................................................................................49

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Navigating VuSitu.............................................................................................................................................................................51
VuSitu Mobile App........................................................................................................................................................................51
Connected Instrument Screen.................................................................................................................................................51
VuSitu Menu Options..................................................................................................................................................................52
Selecting with Long-press and Swipe...................................................................................................................................52
HydroVu............................................................................................................................................................................................53
Live Readings in VuSitu..................................................................................................................................................................54
Snapshot Mode..............................................................................................................................................................................54
Live Readings Mode.....................................................................................................................................................................54
VuSitu Data.........................................................................................................................................................................................55
Downloading Your Data.............................................................................................................................................................55
Sharing Data...................................................................................................................................................................................55
Viewing Data on a Mac or PC....................................................................................................................................................55
VuSitu Locations...............................................................................................................................................................................56
About VuSitu Locations..............................................................................................................................................................56
How to Create a Location...........................................................................................................................................................56
How to Select a Location............................................................................................................................................................57
How to Edit or Delete a Location.............................................................................................................................................57
Logging................................................................................................................................................................................................58
How to Create a Log with VuSitu.............................................................................................................................................58
Remote-Monitoring Configuration (Telemetry)....................................................................................................................59
Connecting to Win-Situ on a PC..................................................................................................................................................61
Connecting to Win-Situ via USB...............................................................................................................................................61
Navigating the Win-Situ Interface..............................................................................................................................................62
Home Tab.........................................................................................................................................................................................62
Sensor Tab........................................................................................................................................................................................62
Device Setup Tab...........................................................................................................................................................................63
Logging Tab.....................................................................................................................................................................................63
BaroMerge.......................................................................................................................................................................................64
Connecting the Aqua TROLL 600 to a PLC or Data Logger................................................................................................68
Modbus PLC Interface.....................................................................................................................................................................71

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Care and Maintenance....................................................................................................................................................................74
Maintenance Schedule...............................................................................................................................................................74
User-Serviceable Parts.................................................................................................................................................................74
Storage..............................................................................................................................................................................................74
Cleaning the Sonde......................................................................................................................................................................75
Cleaning and Storing the pH/ORP Sensor...........................................................................................................................75
Cleaning and Storing the RDO Sensor..................................................................................................................................77
Cleaning and Storing the Conductivity Sensor..................................................................................................................78
Cleaning and Storing the Turbidity Sensor..........................................................................................................................79
More Information.............................................................................................................................................................................80
Declaration of Conformity.............................................................................................................................................................81
Appendix ............................................................................................................................................................................................82
Appendix A: Parameter Discovery..........................................................................................................................................82
Appendix B: Parameter Numbers and Locations...............................................................................................................83
Appendix C: Unit IDs....................................................................................................................................................................86
Appendix D: Register Data Formats.......................................................................................................................................89

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Introduction
Serial Number Location
The instrument serial number is on the product label affixed to the instrument body. Serial numbers forindividual sensors are engraved on the sensor body.

Instrument Description
The Aqua TROLL 600 uses the latest sensor and electronics technology to provide laboratory-quality
measurements for field use. Parameters include water level, pH, dissolved oxygen, and more. The instrument
fits inside wells as small as 2” in diameter. The water level/pressure sensor and barometric pressure sensor
are integrated into the sonde. Additional sensors may be selected and replaced to suit your project’s needs.
An optional motorized sensor wiper may also be included on the instrument.
Use the Aqua TROLL 600 for long-term monitoring in freshwater and marine environments. It’s also ideal forthe following applications:• Groundwater sampling• Low-flow testing• Mine water monitoring• Stormwater management
The built-in LCD screen displays battery status, connectivity info, and other vital information. Setup is easy
with the VuSitu app and a Bluetooth-enabled mobile device. The Aqua TROLL 600 can record data to aninternal micro SD card and works with telemetry devices for remote monitoring.

Document Conventions
Throughout this document you will see the following symbols:
A checkmark highlights a tip or feature.

The exclamation point calls your attention to a requirement, safety issue, or important actionthat should not be overlooked.

Unpacking and InspectingYour equipment was carefully inspected before shipping. Check the equipment for any physical damage
sustained during shipment. Notify In-Situ and file a claim with the carrier if there is any such damage; do not
attempt to deploy or operate the instrument.
Save packing materials for future storage and shipping of your equipment.
Accessories may be shipped separately and should be inspected for physical damage and order fulfillment.

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Obtaining Repair Service
If you suspect your system is malfunctioning and repair is needed, you can help assure efficient servicing byfollowing these guidelines:
1 Call or email In-Situ Technical Support. Have the product model and serial number available.
2 Be prepared to describe the problem, including how the product was used and the conditions notedat the time of the malfunction.
3 If Technical Support determines that service is needed, they will ask your company to fill out the RMA
form and pre-approve a specified monetary amount for repair charges. When the form and pre-approvalis received, Technical Support will assign an RMA (Return Material Authorization) number.
4 Clean the product as described in the manual.
5 If the product contains a removable battery, remove and retain it unless you are returning the system
for a refund or Technical Support states otherwise.
6 Carefully pack your product in its original shipping box, if possible.
7 Mark the RMA number clearly on the outside of the box.
8 Send the package, shipping prepaid, to:In-Situ:
ATTN: Repairs
221 East Lincoln Avenue
Fort Collins, CO 80524
The warranty does not cover damage during transit. In-Situ recommends insurance for all shipments.
Warranty repairs will be shipped back prepaid.
Outside the U.S.
Contact your international In-Situ distributor for repair and service information.

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Guidelines for Cleaning Returned Equipment
Please help us protect the health and safety of our employees by cleaning and decontaminating
equipment that has been subjected to potential biological or health hazards, and labeling such equipment.
Unfortunately, we cannot service your equipment without such notification. Please complete and sign theform (or a similar statement certifying that the equipment has been cleaned and decontaminated) and send
it to us with each instrument.
1 We recommend cleaning with only mild soaps that are compatible with the product materials.
Wetted materials lists are provided on the product specification sheets. Clean all cables and remove all
foreign matter.
2 Clean the cable connectors with a clean, dry cloth. Do not submerge the connectors.
3 Clean the instrument including the nosecone, cable head, and protective caps.

If an instrument is returned to our Service Center for repair or recalibration without astatement that it has been cleaned and decontaminated, or if it is the opinion of our Service
Representatives that the equipment presents a potential health or biological hazard, we
reserve the right to withhold service until proper certification is obtained.


Decontamination & Cleaning Statement
Company Name

Phone

Address
City

State

Instrument Type

Serial Number

Contaminant(s) if known)

Decontamination procedure(s) used

Cleaning verified by

Title

Date

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Safety
Read the safety information on this page before deploying or configuring your Aqua TROLL
600. If you have questions, contact In-Situ Technical Support for assistance.
• Use D-cell alkaline batteries.
• Do not use the Aqua TROLL 600 in any manner not specified by the manufacturer.
• Do not use batteries of different ages or types.
• Do not submerge the Twist-Lock connector ends of the cable or instrument when they are not connected.
• Do not submerge the Wireless TROLL Com or your mobile device in liquid.
• Ensure that sensors or sensor plugs, are completely inserted into the ports, so that no liquid can enter the
instrument.
• Ensure that the RDO Sensor Cap is pressed firmly over the sensor lens and is flush with the instrument
before submerging in liquid.
• Replace the cable if insulation or connectors are damaged.
• Make sure the probe and sensor O-rings are clean and free of damage.

Intended Use
The Aqua TROLL 600 multiparameter sonde is designed to be safe:• during indoor or outdoor use;
• when used at altitudes up to 2000m;• in ambient temperatures from -5 to 50C;• in any relative humidity levels;• during transient voltages up to the levels of Overvoltage Category III.
If the equipment is used in a manner not specified by the manufacturer, the protection
provided by the equipment may be impaired.

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Instrument Specifications
Operating temperature
Storage temperature
Dimensions-5 to 50° C (23 to 122° F)
Components without fluid: -40 to 65° C (-40 to 149° F) pH/ORP probes:-5 to 65° C
4.7 cm (1.85 in.) OD x 60.2 cm (23.7 in.) with restrictor installed(including connector)
With bail: 73.9 cm (29.1 in.)

Weight1.45 kg (3.2 lbs) - includes all sensors, batteries, bailWetted materials(sonde and sensors)

Ceramic, Delrin™, Inconel™, PC, PC alloy, Platinum, Nylon,
Santoprene™,Titanium, Viton™
IP68 with all sensors and cable attached. IP67 with sensors removed,
battery cover removed, or cable detached

Environment rating
Max pressure rating

Up to 350 PSICommunication

RS485/MODBUS, SDI-12, Bluetooth®

Reading rate

1 reading every 2 seconds for one parameter, no wipe

Internal memory

16 MB

Additional memory, micro SD
card2

16+ GB included, any size micro SD up to 32 GB accepted

Logging rate

1 minute to 99 hours

Logging modes

Linear, Linear Average, Event

Data logging

50 logs (defined, scheduled to run, or stored)

LCD screen
Internal power
Battery life3
External power voltage4
External power current4
Interface

Integrated display shows status of sonde, sensor ports, data log,
battery, and connectivity(2) user-replaceable D-cell alkaline batteries> 6 months typical with wiping> 9 months typical without wiping
8-36 VDC (required for normal operation)
Sleep: < 0.2 mA typical
Measurement: 40 mA typical, 75 mA Max
Win-Situ 5 Software, VuSitu Mobile App on selectmobile devices using Android 4.4 with Bluetooth 2.0

Cable

Vented or non-vented polyurethane or vented Tefzel®

Hex screw driver

0.050 in. (1.3 mm)

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Software interface

Android: VuSitu through Google Play
Windows: Win-Situ 5
Data Services: HydroVu

Interface

Android 4.4, requires BlueTooth 2.0

Certifications

CE, FCC, WEEE, RoHS Compliant
2 year - sonde, sensors (excluding pH/ORP); 1 year - pH/ORP sensor
Other - see warranty policy at www.in-situ.com
Specifications are subject to change without notice. Android is atrademark of Google, Inc. Bluetooth is a trademark of Bluetooth SIG,
Inc. Delrin and Tefzel are trademarks of E.I. du Pont de Nemours & Co.
Santoprene is a trademark of ExxonMobile. Inconel is a trademark ofSpecial Metals Corporation. Viton is a registered trademark of DuPont
Performance Elastomers L.L.C.

Warranty

Notes

For 30 parameters>100,000 data records, > 3 years at 15 minute interval. A single data record includes timestamp, temperature,
RDO, pH, ORP, turbidity, and conductivity logged in Linear or Linear Average mode.

1

Log data recorded to SD card in comma delimited variable (CSV) file format.

2

Logging all sensors at 15 minute interval on 2 D-cell alkaline batteries. Battery life dependent on site conditions and wiping.

3

Dependent on display and wiping

4External Power Source Requirements
Current from an external power source must not exceed 4 amps.

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Instrument Dimensions with Restrictor On

Total length with connector

60.2 cm (23.7 in)Diameter

47 mm (1.85 in)

Instrument Dimensions with Restrictor Off

Total length with wiper brush on

60.2 cm (23.7 in)Total length with wiper brush off

47 mm (1.85 in)Diameter

41.66 mm (1.64 in)

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Sensor Specifications
Sensor Summary
Usable Depth

Operational
Temperature
Range

350

200

650

- 5 to 50° C

12 months**

350

200

650

- 5 to 50° C

2 years or greater

User calibration only ifneeded

350

200

650

- 5 to 50° C

Temperature

2 years or greater

NA

350

200

650

- 5 to 50° C

Turbidity

2 years or greater

User calibration only ifneeded

350

200

650

- 5 to 50° C

Pressure

2 years or greater

User calibration only ifneeded

12.8
42.7
108
285

9
30
76
200

30
100
250
650

- 5 to 50° C

BarometricPressure

2 years or greater

User calibration only ifneededNA

NANA- 5 to 50° C

Ammonium

6 to 12 months**

Monthly**

30

25

70

0 to 40° C

Chloride

1 year or greater**Monthly**

350

200

650

0 to 50° C

Nitrate6 to 12 months**

Monthly**

30

25

70

0 to 40° C

Chlorophyll a

2 years or greater

User calibration only ifneeded

350

200

650

- 5 to 50° C

BGA-PC

2 years or greater

User calibration only ifneeded

350

200

650

- 5 to 50° C

BGA-PE

2 years or greater

User calibration only ifneeded

350

200

650

- 5 to 50° C

Rhodamine

2 years or greater

User calibration only ifneeded

350

200

650

- 5 to 50° C

Fluorescein

2 years or greater

User calibration only ifneeded

350

200

650

- 5 to 50° C

FDOM

2 years or greater

User calibration only ifneeded

350

200

650

- 5 to 50° C

Crude OIl

2 years or greater

User calibration only ifneeded

350

200

650

- 5 to 50° C

Expected Lifetime*

Recommended
Calibration Frequency

Pressure
Rating - PSI

pH/ORP

2 years or greater**

10 to 12 weeks**RDO

2 years or greater

Conductivity

Sensors* Expected lifetime includes total shelf life and deployment lifetime.
** Lifetime and calibration frequency depend on site and storage conditions.

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Solutions
Solution

Shelf Life - Unopened

Shelf Life - Opened

Quick Cal

4 months. Store in a cool, dark place. Shakebefore use.

7 to 21 days (±10 mV, ±0.05 pH,±50 μS/cm)

ZoBell’s

9 months. Store in a cool, dark place.

3 to 6 months

Low Conductivity (147
μS/cm)

12 months

Hours (±1 μS/cm, check before
use)

Other Conductivity

12 months

3 to 6 months

pH Calibration Buffers

24 months

3 to 6 months

Sensor Reference Filling
Solution

24 months

12 months

pH Storage Solution

24 months

12 months

Sodium Sulfite

12 months

3 to 6 months

Turbidity

12 months

12 months from expiration date

Deionized Water

24 months

Hours, check before use forcalibration

Ammonium

12 months

3 to 6 months

Chloride

12 months

3 to 6 months

Nitrate12 months

3 to 6 months

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Potential Interferents
pH
Sodium salts

Conductivity
Temperature

Dissolved Oxygen
Temperature, atmospheric pressure, salinity,
chlorinity

ORP
Ions that are stronger reducing agents thanhydrogen or platinum, e.g., chromium, vanadium,titanium, etc.

Ammonium
Cesium, Potassium, Thallium, pH, Silver, Lithium,
SodiumNitratePerchlorate, Iodide, Chlorate, Cyanide, Bromide,
Nitrite, Hydrogen Sulfide (bisulfite), Hydrogen
Carbonate(bicarbonate), Carbonate, Chloride, Dihydrogen
Phosphate, Hydrogen Phosphate, Phosphate,
Acetate, Fluoride, Sulfate

ChlorideHydroxide, Ammonia, Thiosulfate, Bromide, Sulfide,
Iodide, Cyanide
BGA-PC, BGA-PE, Chlorophyll a, Rhodamine WT
Turbidity

RDO Cap Chemical Incompatibility
The following chemicals will damage the RDO sensing element.
•

Alcohols > 5%•

Hydrogen peroxide > 3%•

Sodium hypochlorite (commercial bleach) > 3%•

Gaseous sulfur dioxide•

Gaseous chlorine• Do not use in organic solvents (e.g., acetone, chloroform, methylene chloride, etc.), which may destroy
the sensing element

Conductivity/Temperature Sensor
Soaking the Conductivity/Temperature sensor in vinegar for longer than one hour can cause
serious damage.

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Ammonium, Chloride, and Nitrate Interferent Concentrations
Ammonium
The table below lists concentrations of possible interfering ions that cause 10% error at various levels (inppm) of NH4+.
Ion

100 ppm NH4+

10 ppm NH4+

1 ppm NH4+

Celsium (Cs+)

100

10

1

Potassium (K+)

270

27

2.7

Thallium (TI+)

3100

310

31

pH (H+)

pH 1.6pH 2.6pH 3.6

Silver (Ag+)

270,000

27,0002,700

Lithium (Li+)

35,0003,500

350

Sodium (Na+)

11,1001,100

110Chloride
The table below lists concentrations of possible interfering ions that cause 10% error at various levels (inppm) of Cl-.
Ion

100 ppm Cl-

10 ppm Cl-

1 ppm Cl-

Hydroxide (OH-)

3,840

384

38.4

Ammonia (NH3)

6

0.6

0.06

Thiosulfate (S2O32-)

3

0.3

0.03

Bromide (Br-)

0.68

0.068

6.8 x 10-3

Sulfide (S2-)

9 x 10-5

9 x 10-6

9 x 10-7

Iodide (I-)

1.8 x 10-4

1.8 x 10-5

1.8 x 10-6

Cyanide (CN-)

1.5 x 10-5

1.5 x 10-6

1.5 x 10-7

Nitrate
The table below lists concentrations of possible interfering ions that cause 10% error at various levels (inppm) of N03-.
Ion

100 ppm NO3-

10 ppm NO3-

1 ppm NO3-

7 x 10-2

7 x 10-3

7 x 10-4

Iodide (I-)

4

0.4

0.04

Chlorate (CIO3-)

30

3

0.3

Cyanide (CN-)

20

2

0.2

Bromide (Br-)

400

40

4

Nitrite (NO2-)

230

23

2

Hydrogen Sulfide (HS-)

230

23

2

Perchlorate (CIO4-)

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Bicarbonate (HCO3-)

440

440

44

Carbonate (CO32-)

8,600

860

86

Chloride (CI-)

7,600

760

76

34,6403,464

346

34,3003,430

343

Phosphate (PO43-)

33,9003,390

339

Acetate (OAc-)

104,200

10,420

1,042

Fluoride (F-)

81,4008,140814

Sulfate (SO42-)

685,700

68,5706,857

Dihydrogen Phosphate(H2PO4-)
Hydrogen Phosphate(HPO42-)

Barometric Pressure Sensor Specifications
Accuracy±1.0 mbar

Range

300 to 1,100 mbar

Resolution

0.1 mbarSensor Type

Fixed

Response Time

T63<1s, T90<1s, T95<1sUnits of Measurepsi, kPa, bar, mbar, mmHg, inHg

Methodology

Silicon strain gauge

Conductivity Sensor Specifications

Accuracy±0.5% of reading plus 1 μS/cm from 0 to 100,000
μS/cm;±1.0% of reading from 100,000 to 200,000
μS/cm; ±2% of reading from 200,000 to 350,000 µS/
cm

Range

0 to 350,000 μS/cm

Resolution

0.1 µS/cm

Sensor Type

Removeable

Response Time

T63<1s, T90<3s, T95<5s

Units of Measure

Actual conductivity: μS/cm, mS/cm Specificconductivity: μS/cm, mS/cm Salinity: PSU
Total dissolved solids: ppt, ppm Resistivity: Ohmscm
Density: g/cm3

Methodology

Std. Methods 2510, EPA 120.1*Accuracy at calibration points. For greatest accuracy over instrument life, keep the conductivity cell submersed in water for 24-48
hours prior to calibration and deployment.

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Total Dissolved Solids
TDS is derived from conductivity and temperature.
Range

0 to 350 ppt

Resolution

0.1 ppt

Units of Measureppt, ppm

Salinity
TDS is derived from conductivity and temperature.
Range

0 to 350 PSU

Resolution

0.1 PSU

Units of Measure

PSU, ppt

Methodology

Std. Methods 2520A

Dissolved Oxygen RDO Sensor Specifications
AccuracyRange±0.1 mg/L from 0 to 8 mg/L
±2% of reading from 20 to 50 mg/L
0 to 20 mg/L
20 to 60 mg/L

Resolution

0.01 mg/L

Sensor Type

Removable with replaceable RDO-X Cap

Response Time

RDO-X Cap: T63<15s, T90<45s, T95<60s
Fast Cap: T63<3s, T90<30s, T95<45s

Units of Measure

mg/L, % saturation, ppm

Methodology

EPA-approved In-Situ Methods (under the Alternate
Test Procedure process): 1002-8-2009, 1003-8-2009,1004-8-2009

Level, Depth, Pressure Sensor Specifications
Accuracy

Typical ±0.1% full scale (FS)

Range

Non-vented or Vented
9.0 m (30 ft) - Burst: 27 m (90 ft) 30 m (100 ft) - Burst:
40 m (130 ft) 76 m (250 ft) - Burst: 107 m (350 ft) 200m (650 ft) - Burst: 229 m (750 ft)

Resolution±0.01% FS or better

Sensor Type

Fixed

Response Time

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Units of Measure

Pressure: psi, kPa, bar, mbar, mmHg, inHg, cmH2O,
inH2O Level: mm, cm, m, in, ft, cmH2O, inH2O

Methodology

Piezoresistive; ceramic*Typical performance across full temperature and pressure calibrated range. Typical is defined as all values
within 1 standard deviation.
ORP Sensor Specifications
Accuracy±5.0 mV @ 25° C

Range±1,400 mV

Resolution

0.1 mV

Sensor Type

Replaceable pH/ORP combo sensor

Response Time**

T63<3s, T90<15s, T95<30s

Units of MeasuremV

Methodology

Std. Methods 2580

*Accuracy from standard at 25° C.
**At thermal equilibrium immediately following calibration, measuring from air to +400 mV
pH Sensor Specifications
Accuracy±0.1 pH units or better

Range

0 to 14 pH units

Resolution

0.01 pH unit

Sensor Type

Replaceable pH/ORP combo sensor

Response Time**

T63<1s, T90<2s, T95<3sUnits of Measure

pH units

Methodology

Std. Methods 4500-H+, EPA 150.2*At thermal equilibrium.
Temperature Sensor Specifications
Accuracy±0.1° CRange

-5 to 50° C (23 to 122° F)

Resolution

0.01° C

Sensor Type

Replaceable

Response Time**

T63<2s, T90<15s, T95<30s

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Units of Measure° C, ° F

Methodology

EPA 170.1

Sensor only, when transferring from air to ambient water temperature. Typical system response time with all
sensors and restrictor installed: T63<30s; T90<3.5m; T95,7.5m
Turbidity Sensor Specifications
Accuracy±2% of reading or ±0.5 NTU or FNU, whichever isgreaterRange

0 to 4,000 NTU

Resolution

0.01 NTU (0 to 1,000 NTU) 0.1 NTU (1,000 to 4,000
NTU)

Sensor Type

Replaceable

Response Time**

T63<1s, T90<1s, T95<1sUnits of Measure

NTU, FNU

Methodology

ISO 7027

Total Suspended Solids
TSS is derived from turbidity.
Range

0 to 1,500 mg/L

Resolution

0.1 mg/L

Units of Measureppt, mg/L

User-defined reference.
Ammonium Sensor Specifications
Accuracy±10% or ± 2 mg/L, whichever is greater

Max Depth

25 m, 30 PSI

Range

0-10,000 mg/L as N

Resolution

0.01 mg/L

Sensor Type

Removable

Response Time*T90 < 10sec, T95 < 30sec

Units of Measure

mg/L, ppm, mV

Methodology

Std. Methods 4500-NH3D, EPA 350.3*Between calibration points.

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Ammonia (Unionized Ammonia and Total Ammonia)
Ammonia is derived from ammonium, pH and salinity. pH sensor andconductivity/temperature sensor required.
Range

0 to 10,000 mg/L

Resolution

0.01 mg/L

Units of Measure

mg/L, ppm

Chloride Sensor Specifications
Accuracy±10% or ±2 mg/L, w.i.g.*

Range

0-190,000 mg/L - CL-

Resolution

0.01 mg/L

Sensor Type

Removable

Response Time*T90 < 10sec, T95 < 30sec

Units of Measure

mg/L, ppm, mV

Methodology

Std. Methods 4500-Cl-D* Between calibration points.
Nitrate Sensor Specifications
Accuracy±10% or ±2 mg/L, w.i.g.*

Max Depth

25 m, 30 PSI

Range

0-50,000 mg/L as N

Resolution

0.01 mg/L

Sensor Type

Removable

Response Time*T90 < 10sec, T95 < 30sec

Units of Measure

mg/L, ppm, mV

Methodology

Std. Methods 4500-NO3D* Between calibration points.
Chlorophyll a Sensor Specifications
Linearity

R2 > 0.999 for serial dilutions of 0-1000 µg/L Chl a in
MeOH

Max Depth

200 m

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RangeResolution

0-100 RFU
0-1000 µg/L Chl a in MeoH
.001 RFU.01 µg/L Chl a

Sensor Type

Removable

Response Time*

T63<1s, T90<1s, T95<1sUnits of Measure

Concentration: µg/L
Fluorescence: RFU

Excitation Wavelength (nominal)

430 nmDetection Wavelength

675 nm to 750 nm

BGA-PC Sensor Specifications
Linearity

R2 > 0.999 for serial dilutions of PC standards from
0-1000 µg/L PC

Max Depth

200 m

Range
Resolution

0-100 RFU
0-1000 µg/L PC.001 RFU.01 µg/L PC

Sensor Type

Removable

Response Time*

T63<1s, T90<1s, T95<1sUnits of Measure

Concentration: µg/L
Fluorescence: RFU

Excitation Wavelength (nominal)

590 nmDetection Wavelength

640 nm to 690 nm

BGA-PE Sensor Specifications
Linearity

R2 > 0.999 for serial dilutions of PE standards from
0-1000 µg/L PE

Max Depth

200 m

Range
Resolution

0-100 RFU
0-1000 µg/L PE.001 RFU.01 µg/L PE

Sensor Type

Removable

Response Time*

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Units of Measure

Concentration: µg/L Fluorescence: RFU

Excitation Wavelength

498 nmDetection Wavelength

575 nm to 625 nm

Rhodamine WT Sensor Specifications
Linearity

R2 > 0.999 for serial dilutions of RWT (Rhodamine
Water Tracer) standards from 0-1000 µg/L

Max Depth

200 m

Range
Resolution

0-100 RFU
0-1000 µg/L
.001 RFU.01 µg/Sensor Type

Removable

Response Time*

T63<1s, T90<1s, T95<1sUnits of Measure

Concentration: µg/L
Fluorescence: RFU

Excitation Wavelength (nominal)

530 nmDetection Wavelength

580 nm to 660 nm

Fluorescein WT Sensor Specifications
Linearity

R2 > 0.999 for serial dilutions of FWT (Fluorescein
Water Tracer) standards from 0-500 µg/L

Max Depth

200 m

Range
Resolution

0-100 RFU
0-500 µg/L
.001 RFU.005 µg/L

Sensor Type

Removable

Response Time*

T63<1s, T90<1s, T95<1sUnits of Measure

Concentration: µg/L
Fluorescence: RFU

Excitation Wavelength (nominal)

462 nmDetection Wavelength

525 nm to 570 nm

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FDOM Sensor Specifications
Linearity

R2 > 0.999 for serial dilutions of Quinine Sulfate from0-3000 µg/L

Max Depth

200 m

Range

0-100 RFU
0-3000 µg/L

Resolution.001 RFU.03 µg/L

Sensor Type

Removable

Response Time*

T63<1s, T90<1s, T95<1sUnits of Measure

Concentration: µg/L
Fluorescence: RFU

Excitation Wavelength (nominal)

375 nmDetection Wavelength

455 nm to 530 nm

Crude Oil Sensor Specifications
Linearity

R2 > 0.999 for serial dilutions of PTSA from 0-3000µg/L

Max Depth

200 m
0-100 RFU
0-3000 µg/L
.001 RFU.03 µg/L

Range
Resolution
Sensor Type

Removable

Response Time*

T63<1s, T90<1s, T95<1sUnits of Measure

Concentration: µg/L
Fluorescence: RFU

Excitation Wavelength (nominal)

365 nmDetection Wavelength

430 nm to 505 nm

Warranty

2 year - Sonde, RDO and sensor cap, temperature/conductivity, temperature
only, turbidity (excluding pH/ORP)
1 year - pH/ORP, chloride ISE, accessories
90 Days - Nitrate and Ammonium ISE sensors
Other: see warranty policy (www.in-situ.com/warranty)

Notes

Specifications are subject to change without notice. Android is a trademarkof Google, Inc. Bluetooth is a trademark of Bluetooth SIG, Inc. Delrin and Tefzel
are trademarks of E.I. du Pont de Nemours & Co. Santoprene is a trademark of
ExxonMobile. Inconel is a trademark of Special Metals Corporation. Viton is a
registered trademark of DuPont Performance Elastomers L.L.C.

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Required Accessories
Communication Device
You can use a communication device to calibrate, configure and deploy the Aqua TROLL 600.

Wireless TROLL Com
Provides power to the Aqua TROLL 600 to extend battery life.
Configure and deploy with a Bluetooth-endabled Android device.
Connects the Aqua TROLL 600 to a PC via USB.

Cable
Rugged Twist-Lock Cable
Connects the Aqua TROLL 600 to a Wireless TROLL
Com, USB TROLL Com or VuLink.
Vented or non-vented.

Sensors

Available Sensors
1 Temperature
2 Conductivity/temperature
3 pH/ORP
4 RDO
5 Turbidity
6 Ammonium
7 Chloride
8 Nitrate
9 Chlorophyll a
10 BGA-PC
11 BGA-PE
12 Rhodamine WT
13 Fuorescein WT
14 FDOM
15 Crude Oil

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Software
Win-Situ 5 Software
for PC

VuSitu Mobile App
Calibrate, configure and deploy the
Aqua TROLL 600 from a
Bluetooth-enabled Android device.

Calibrate, configure andtake readings with theAqua TROLL 600 from aPC.

Download it from www.in-situ.com.

Get it at play.google.com.

Telemetry
VuLinkPower Aqua TROLL 600 inremote-monitoring
applications
Calibrate, configure, anddeploy the Aqua TROLL 600.
Send data to HydroVu or an
external FTP Server

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System Components
Base Unit Components
Component

Part Number

RDO Sensor - includes RDO-X Cap

0063450

Combination pH/ORP Sensor

0063470

Turbidity Sensor

0063480

Combination Conductivity/Temperature Sensor or
standalone Temperature Sensor
Alkaline Batteries (2)0063460, 0063490

Dual Stainless Steel Restrictor/Storage Chamber

0079820

Sensor Port Plugs (2)0063510

Rubber Bumpers (2)0079880

Wiper or Wiper Port Plug0063500, 0064630

0042020

Additional Sensors
Ammonium Sensor

0033700

Nitrate Sensor

0033710Chloride Sensor

0033720

Chlorophyll A Sensor

0038900

Phycocyanin (BGA-PC) Sensor

0038920

Phycoerythrin (BGA-PE) Sensor

0038930

Rhodamine WT Sensor

0038890

Fluorescein WT Sensor

0096050

Crude Oil Sensor

0096060

Fluorescent Dissolved Organic Matter (FDOM)
Sensor0096070

Communications
Wireless TROLL Com for Android

0031240

Mobile Device for Android

0064860

TROLL Com RS-232 Cable Connect0056140

TROLL Com USB Cable Connect

0052500

TROLL Com RS-232 Direct Connect0056150

TROLL Com USB Direct Connect

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CableStripped-and-tinned Cable with male connector

0053310

Twist-lock Bulkhead Connector

0053240

Twist-lock Backshell/Hanger, Titanium

0051480

Cable Extender0051490

Large Desiccant (titanium connector)

0051810Large Desiccant (ABS connector)

0053550

Small Desiccant (3 pack) - storage desiccant

0052230

Desiccant Refill Kit for Large or Outboard Desiccant

0029140

Calibration and Maintenance
RDO Classic Cap Replacement Kit

0079790

pH/ORP Replacement Reference Junction Kit

0078990

Wiper Brush Kit

0079810Maintenance Kit

0078940

Copper Antifouling Guard

0076100

Quick-Cal Solution for calibrating DO, Cond., pH &ORP
Dissolved Oxygen Calibration Kit

0033250

D.O. Field Calibration Kit

0080830

Conductivity Calibration Kit (Full)

0032090

Conductivity Calibration Kit (Low)0032630

Conductivity Calibration Kit (High)0032640

pH Calibration Kit

0032080

pH/ORP Calibration Kit

0032120

pH Storage Solution

0065370

Individual Calibration Solutions

See website

Ammonium Calibration Kit (includes 1 liter each: 14ppm, 140 ppm, 1400 ppm, DI water)
Chloride Calibration Kit (includes 1 liter each: 35.5
ppm, 355 ppm, 3545 ppm, DI water)
Nitrate Calibration Kit (includes 1 liter each: 14 ppm,
140 ppm, 1400 ppm, DI water)
Fluorescein WT Solid State RFU Calibrator

0032140FDOM Solid State RFU Calibrator

0104300

Crude OIl Solid State RFU Calibrator

01043101-970-498-1500

003211000321500032130
010429028

www.in-situ.com

Low-Flow
Complete Low-Flow Kit (includes flow cell withfittings and stake, base plate, documentation)
Flow Cell Kit

0079790

Flow Cell Base Plate

0079810

Fittings Kit

0032130

Pelican Case with Foam Insert

0066860

1-970-498-1500

007899029

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RuggedCable System
RuggedCable Systems are custom-built, durable, direct-read cables that include the following items:• Titanium twist-lock connectors for quick, reliable connections to the instrument, desiccant, andcommunication cable•

Metal shield beneath the cable jacket to prevent electrical interferences•

Kellems grip for secure instrument deployment•

Small desiccant for vented systems (for storage only)
Non-vented cables are marked with VF, which means vent free.

Vented or Non-Vented Cable
Vented cable is used with vented pressure sensors to produce gauged measurements. The cable vent tube
ensures that atmospheric pressure is applied to the back of the sensor diaphragm.
Non-vented cable is used with non-vented instruments for absolute measurements. Compensate absolutemeasurements by using a BaroTROLL Instrument and Win-Situ Baro Merge Software.
Vented cable is shipped with a small desiccant to protect against condensation. Larger
desiccants are necessary for deployment.
Jacket Options
Tefzel (vented) or thermoplastic polyurethane (TPU, vented or non-vented)
Customizable Cable Lengths
Cables can be ordered up to 1,219 m (4,000 ft).
Cable Termination
Cables can be ordered with a twist-lock termination (female connector) on both ends that connect to the
instrument, the TROLL Com Communication Device, desiccant, and other accessories.
Cables can also be ordered with stripped-and-tinned termination for wiring to a data logger or controller
using SDI-12, analog (4-20 mA), or Modbus communication protocol.

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RuggedCable System with female to female connectors

2

Stripped-and-tinned RuggedCable System with female connector

3

Stripped-and-tinned RuggedCable System with male connector (shortlength that converts a cable with a twist-lock connector to a strippedand-tinned cable)

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Aqua TROLL 600 Quickstart Guide
Box Contents
1 Documentation and software
2 Wiper motor and brush/wiper
3 Aqua TROLL 600 sonde
4 Water quality sensors (2 of 4)
5 Water quality sensors (2 of 4)
6 RDO sensor cap
7 Accessory supplies
8 pH/ORP sensor maintenance supplies
9 Extra sensors
10 D-cell alkaline batteries
11 Instrument tools

Getting Started
1

Install the batteries.

Open the battery
compartment.

Install alkaline batteries.

Use Allen wrench to
remove and checkdesiccant color. If pink,replace.

2

Install the wiper motor and sensors.

abRemove restrictor.

Apply a pea-sized drop of
lubricant to O-rings.

Close battery
compartment. LCD
screen should activate.

c
Install wiper motor or port plug
into center port.
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de

Apply a pea-sized drop of
lubricant to O-rings.

f

Align sensor with interlock groovein wiper motor and slide sensor
into port.

ghUnscrew end cap from restrictor.

Tighten set-screw at base of eachsensor.

i

Flip restrictor and install withrestrictor holes near center of
instrument for calibration.

Flip restrictor and replace end cap
before deployment.

When using a conductivity sensor and turbidity sensor together, install them side-by-side to
maximize performance.

3

Connect the cable to the instrument.

Remove protective caps
from instrument andcable.

Ensure O-ring on
instrument connector
is clean. Apply vacuum
grease to O-ring.

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Flat edge inside cableend must align withflat edge on instrument
connector.

32

Hold textured sleeve ofcable in one hand andinstrument in other. Pushand twist until click.

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4Connect the communication device.
6 or

If desiccant is present,
remove it from cable.

5

Align TROLL Com
connector with cableend. Push and twist until
click.

Connect VuSitu directly
to instrument. Holdsonde vertically withsensors up. Screen will
activate.

Connect to the software.
You must have the VuSitu mobile app to use the instrument with a mobile device. Download
VuSitu from the Google Play Store or the Apple App Store.

iOS

Androidaab

An iOS device
automatically connects
to the closest In-Situ
instrument.

Launch VuSitu and tap
Connect.

To connect to another
instrument, press
Disconnect and thenChoose or Add Device.
VuSitu displays a list ofavailable connections.

c
Tap the serial numberof the instrument or
Wireless TROLL Com.

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The app locates anddisplays nearby In-Situ
devices.

d
VuSitu displays theConnected Instrument
screen when pairing iscomplete.

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Handling pH and Ion-Selective Electrode Sensors (ISEs)
Salt may accumulate around the reference junctions of the ammonium, chloride, nitrate andpH sensors. Rinse with deionized water to remove any buildup.

Potential salt buildup. Rinse with deionizedwater if necessary.

Before using the ISE sensors for the first time, replace the reference filling solution. Condition
the sensors by soaking in calibration standard for 4-24 hours prior to deployment. This step is
not necessary for the pH sensor.

1 Unscrew reference junction.
2 Replace reference filling solution.

Using the RDO Sensor and RDO Fast Cap
The wiper can severely reduce the life of the RDO Fast Cap. Wear will vary by application. Verify
sensor performance prior to use and replace the Fast Cap if damaged.

Inspect foil prior to each use. If
damaged, replace cap.

RDO Fast Cap

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Ion-Selective Electrode Sensor (ISE) Requirements
ISE sensors require one or more additional sensors to function. See the details below and install
the required sensors to use an ISE.

Ammonium Sensor Requirements
Conductivity/
Temperature Sensor

Temperature Sensor

or

To calculate ammonia...
Conductivity/
Temperature Sensor

pH Sensor

andChloride, Nitrate, and pH/ORP Sensor Requirements
Conductivity/
Temperature Sensor

Temperature Sensor

or

Do not look directly at the sensor LED or point it at the eyes. Doing so can cause eye damage.

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LCD Screen
View instrument status, change settings, start and stop logs via the LCD screen.

Accessing the LCD Menuab

Hold the instrument verticallywith the sensor end facing up to
activate the LCD screen.

cHold instrument horizontally andslowly tap Aqua TROLL 600 logo
3-4 times to view the main menu.

Tilt instrument left or right toscroll through menu options.
LCD Status Indicators1de

Select an item when its
background turns black bytapping the instrument once.

2

3

4

1 Port Status

You can enable Bluetoothcommunication directly with thesonde via the Bluetooth menu
option.

2 Power Status
3 Log Status
4 Connection Status

Possible Port Statuses
Sensors installed

Port plugs installed

Sensor/port error

External power provided

Battery below 10% orBattery error

Possible Power Statuses
Battery level indicator

Possible Log Statuses
Log is RunningLog is Paused

Log is Scheduled

No Log Set Up

Possible Connected Statuses
Connected via Bluetooth

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Error Messages
Port(s) empty

Cap expiration

Full-Text Messages
The LCD will display text messages instead of status icons when certain conditions are met. Multiple
messages will rotate and display for 3 seconds each.
Text Message
Close battery coverInstall wiper
Install sensors
Install temperature

Cause and Remedy
Battery cover is not fully closed.
Ensure the battery cover is securely tightened.
Wiper sensor port is open.
Install wiper or wiper port plug into center port.
Sensor ports are open.
Install sensors or sensor port plugs.
No Temperature or Conductivity/Temperature sensor detected. Install asensor with Temperature.

Install RDO CapRDO Cap not detected on RDO sensor. Install RDO cap.

RDO Cap expired

RDO cap is no longer valid.
Install a new RDO Cap.

RDO Cap XXX days

Temporary text message update on the lifespan of the RDO Cap.

Starting a Log with the LCD Menu
The data log menu allows you to create and start a basic linear data log if one doesn’t alreadyexist on the instrument.

aActivate the LCD menu and select Data Log.

b

Choose New.

cd

Stopping a Log
Set the logging interval.

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The log starts immediately.

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You can’t update firmware while a log is running. Stop a log to update the sonde or sensors.

Stopping a Log

aActivate the LCD menu and select Data Log.

b

Choose Stop.

c

Select Confirm to stop the log.

d

The log stops immediately.

Adjusting the LCD Menu Contrasta

Activate the LCD menu and scroll to Display. Tap
once to select.

b

Select Contrast.

cTilt the sonde to the left to decrease the contrast. Or,
tilt the sonde to the right to increase contrast. Tap
the Aqua TROLL 600 logo to set the contrast.

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Setting or Changing Menu LanguageabAccess the language menu.

Highlight the desired language and tap.

c

The LCD screen confirms the new language.

Viewing the Updates Menuab

Access the updates menu.

Highlight the desired component to update (Sonde,
Sensor 1, 2, 3, or 4).

c

If the firmware is already up to date, the LCD displays
message indicating that an update isn’t available.
Select Yes if an update is available.
If an update is present on the SD card, the screen will indicate the previous firmware version, aright-facing arrow, and the new version of the firmware.

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Micro SD Card
The Aqua TROLL 600 uses a micro SD card for data storage and updating sonde firmware. You can remove
the SD card and replace it with another for data download, or use the same card. An SD card is not required
to log data.
Removing the Micro SD Card
The micro SD card is housed in a slot near the positive battery terminal and the twist-lock connector.
1 Remove the battery cover from the end of the instrument.
2 Push the micro SD card in towards the instrument to release the card.
3 Remove the card from the slot.
4 To reinstall the card, note the card position diagram in the finger slot. Push the card into the slot until you
hear a click.
Downloading and Deleting Data from the Micro SD Card
1 Remove card from the sonde and insert it into a micro SD card adapter.
2 Insert the adapter into a PC or laptop.
3 Open the micro SD card using the file explorer.
4 Open the folder titled “Serial Number.LOG.” For example, “424690.LOG.”
Logs are named by date and then number. For example, a log started on November 12, 2015
would be named “15111200.CSV” - 15 is the year, 11 is the month, 12 is the day, and 00 is thelog number. If multiple logs are recorded on the same day the last number will increase by one
for each consecutive log.
5 Select the logs you wish to download and move them to the destination.
6 Select the logs you wish to delete and press Delete on the keyboard. Deleted logs cannot be recovered.
Updating Firmware
1 Download the firmware update files to your computer.
2 Remove the SD card from the unit and insert it into your computer.
You may need a micro SD card adapter to connect the SD card to your computer. One isincluded with your Aqua TROLL 600, but any micro SD card adapter can be used.
3 Copy the firmware update files into the ISI.FW file on the SD card.
4 Install the SD card into the instrument and install the battery cover.
5 Turn on the LCD screen by inverting the sonde so the sensor end faces up.
6 Access the instrument menu by tapping the Aqua TROLL 600 logo on the yellow label three times. For
more information on accessing menus, see “LCD Screen.”

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7 Scroll to Updates by tilting the instrument. Access the Updates menu by tapping the Aqua TROLL 600
8 logo once.
9 Scroll to Sonde by tilting the instrument. Tap the Aqua TROLL 600 logo once.
10 The LCD screen will display the current firmware version on the left, then an arrow, and the new firmware
version on the right.
11 Scroll to Yes by tilting the instrument. Tap the Aqua TROLL 600 logo once.
12 The LCD screen will display icons while updating. Once the update is complete the Aqua TROLL 600 and
LCD screen will restart.

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Replacing Desiccant
A replaceable desiccant capsule prevents moisture from damaging the instrument and batteries. Check thecapsule periodically. A pink color indicates expired/exhausted desiccant.

Remove the battery
cover.

Remove the wrench from Insert the wrench into
Insert a new capsule.
the back of the battery
the hole in the back ofReplace the wrench andcompartment.
the battery compartment battery cover.
and push the desiccantcapsule out.

Calibrating Sensors
Solution-Based Calibration
Use the solution-based procedure described below to calibrate all sensors except RDO. You will need thefollowing items.
• Calibration standard, or multiple standards for multi-point calibrations• Wireless TROLL Com connected to the Aqua TROLL 600• Bluetooth-enabled mobile device

1

3

2

Hold the sonde upright to activate In VuSitu, click Calibrations fromthe LCD screen. Pair the sonde
the Connected Instrument screenwith the VuSitu app.
and choose sensor to calibrate.

4
Gently shake the sonde in acircular motion to rinse the insideof restrictor and sensors.

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5

Remove cap from instrument andpour 10-20 ml of DI water into
restrictor.

6

Discard the DI water and repeat
rinsing procedure two moretimes with 10-20 ml of your firstcalibration standard.

42

Follow the instructions in VuSitu
to perform the calibration.

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RDO 100% Saturation Calibration: Water Saturated Air
Use the procedure below to calibrate the Aqua TROLL 600 RDO sensor, or see the next section for analternative method.

SpongeaPlace the restrictor in calibration
mode (holes near center of
instrument).

bc

Saturate a small sponge withwater.

Place the sponge on the restrictor
cap.

5 min

d
Loosely install the end cap,
keeping the sensor face dry andallowing for air flow.

feLeave sponge in restrictor for fiveminutes.

Follow the instructions in VuSitu
to finish calibration.

RDO 100% Saturation Calibration: Saturation Bubbler
Flip
restrictoraFill a 100% saturation bubbler
two-thirds with tap water.

d
Place sonde into bubbler.

1-970-498-1500bcTurn on bubbler and allow 5-10
minutes for 100% saturation.

e

Put sonde into deployment modeby flipping restrictor 180 degrees.

fOpen the VuSitu mobile app andtap Calibrations > RDO Saturation.

43

Follow instructions in VuSitu tofinish calibration.

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RDO Salinity Setting
The Aqua TROLL 600 includes automatic salinity compensation. This feature requires a conductivity sensor
and RDO sensor. With both sensors installed, the sonde uses salinity compensation by default. To change thecompensation value, follow these steps:

Select Instrument
Settings from the menu
at the bottom of thescreen.

From the Instrument
Settings menu, selectSalinity Setting.

Enter your desired
salinity compensation
setting and press Save.

Turbidity Sensor Calibration
You must calibrate the turbidity sensor with In-Situ's turbidity standard, or formazine. The app
may not automatically detect the formazine concentration. Instead, a field will appear in which
you can enter the appropriate value. Select Set User Defined to begin calibrating with the newvalue. If you are using an In-Situ standard and the app does not automatically detect it, performthe sensor cleaning and maintenance procedure, then select Retry Auto Detect.

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Batch Calibrating Sensors in VuSitu
Different Sensor Types (Quick Cal Solution)
Use Quick Cal standard to calibrate conductivity, pH, and ORP with a single solution. Quick Cal
solution can also be used for 100% RDO Saturation calibration. To calibrate multiple sensors of the same
type, follow the instructions on the next page.

1
Install the restrictor in calibration
mode (with holes closest to the
instrument body).

4
Set up the calibration. Make surethat all sensors are completely
submerged.

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3

In VuSitu, click Calibrations from Select sensors to calibrate.
the Connected Instrument screen.
Choose Quick Cal from the menu.

5

6When all sensors have stabilized,
Flip the restrictor back into
tap Accept. Review the calibration deployment mode and install thereport for the completed sensors. end cap.

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Multiple Sensors of the Same TypeFor some parameters (pH, ORP, RDO, turbidity, and conductivity), you can calibrate multiple
sensors of the same type together. Use this feature when you need to batch calibrate a group of sensors
prior to field deployment.

1
Install the sensors to calibrate.
(pH/ORP sensors also require atemperature sensor.) Install the
restrictor in calibration mode.

4
Wait for calibrations to complete.
If one sensor isn’t stablilizing,use the toggle switch to turnthat sensor off and continue
calibrating the others.

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2

3

In VuSitu, click Calibrations from Set up the calibration. Make sure
the Connected Instrument screen. that all sensors are completely
Choose a calibration to perform.
submerged or in the same
calibration environment.

5

6

When all sensors have stabilized,
Remove sensors and install themtap Accept. Review the calibration in the instruments they will bereport for the completed sensors. deployed in.

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Calibration Frequency Recommendations
In-Situ sensors are factory calibrated across the entire range of each sensor, and thus achieve a very highdegree of accuracy and stability for extended periods of time without user calibration. In-Situ recommends
inserting the instrument into a known calibration standard to check the accuracy of a sensor prior toperforming any user calibration if you suspect drift, unless a user calibration is required by a standard
operating procedure.
SensorRecommended User
Calibration Frequency

Recommended Factory
Notes
Calibration Frequency

Conductivity

Only when required byuser protocol

12 months

K-cell value: 0.7 to 1.3

Conductivity +Temperature

Only when required byuser protocol

12 months

K-cell value: 0.7 to 1.3

pH

10 to 12 weeks or as
required by user protocol 12 months
or site conditions

Single point:
Theoretical mV ±30 mV
2- or 3-point Slope:-66 to 50 mV/pH
2- or 3-point Offset:±30mV at pH 7

ORP

10 to 12 weeks or as
required by user protocol 12 months
or site conditions

Offset: ±30 mV

RDO

12 months or as required
12 months
by user protocol

2-point Slope:
0.7 to 1.3
2-point Offset:±0.3 mg/L

Temperature

Only when required byuser protocol

Only when requiredby user protocol

Offset: ±0.5

Turbidity

Only when required byuser protocol

12 months

Pressure/Depth

Only when required byuser protocol

Only when requiredby user protocol<2 times the full scaleaccuracy specification

Barometer

Only when required byuser protocol

Only when requiredby user protocol<2 times the full scaleaccuracy specification

Ammonium

Monthly

N/A

Slope > 20 mV/decade

Chloride

Monthly

N/A

Slope < -20 mV/decade

NitrateMonthly

N/A

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Slope: 0.7 to 1.3

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Chlorophyll a

Only when required byuser protocol

12 months

BGA-PC

Only when required byuser protocol

12 months

BGA-PE

Only when required byuser protocol

12 months

Rhodamine WT

Only when required byuser protocol

12 months

Fluorescein WT

Only when required byuser protocol

12 months

FDOM

Only when required byuser protocol

12 months

Crude Oil

Only when required byuser protocol

12 months

Factory Calibration
Factory calibration includes a thorough cleaning, full functionality check and sensor adjustments to allapplicable sensors over the entire calibrated temperature range. We recommend a factory calibration every
12 months or when the unit appears to drift significantly.

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Zeroing the Depth Sensor
The Aqua TROLL 600 uses its pressure reading and specific gravity value to calculate depth. The pressure sensor is locatedat the center of the instrument, but depth is reported at the smart sensor faces. An embedded gyroscope compensates for the
distance between these sensors and allows the sonde to be deployed in any orientation (vertical, horizontal, angled).

Do not zero the pressure sensor. Erroneous readings will result.

Error Code

UC[0]

FC[0]

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Error Description

Recommended Actions

User Calibration (UC) Expired

1.) Perform a user calibration on thesensor in a known calibration standard
2.) Follow individual sensor instructions
or user SOPs for calibration.

Factory Calibration (FC) Expired

Call (800-446-7488) or contact In-Situ
Technical Support (support@in-situ.com).
Send the instrument back to In-Situ forfactory calibration49

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OL

WU[0]

ERR[0]

DIS[0]

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1.) Remove sensor and check for waterin the connection port. Dry sensor andsonde connector. Reinstall sensors. 2.)
Sensor Office (OL) and no longer
Remove two sensors and reinstall incommunicating with the instrument
separate port. 3.) Call (800-446-7488)
or contact In-Situ Technical Support(support@in-situ.com).
1.) Inspect wiper to ensure surface is
free of debris and the wiper is spinningcorrectly. 2.) Remove wiper brush andinstall a new brush holder with newWiper Error - wiper is not turning
brushes. 3.) Remove the wiper and checkcorrectly or interfering with sensor
for water in the connection port. Dry theperformancewiper port and the sonde connector.
Reinstall wiper. 3.) Call (800-446-7488) orcontact In-Situ Technical Support(support@in-situ.com).
1.) Make sure the sensor is submergedin solution. 2.) Recalibrate sensor in a
fresh batch of calibration standard. 3.)
Sensor is performing outside of expected Reset sensor back to factory defaults andcheck reading in a calibration standard.
range
Recalibrate. 4.) Call (800-446-7488)
or contact In-Situ Technical Support(support@in-situ.com).
1.) Verify that the RDO sensor cap isinstalled, the sensing foil is intact and thecap has not expired. 2.)Clean the surfaceof the sensor to remove any excess
Sensor is not set up correctly, being useddebris 3.) Verify that the instrument andoutside of temperature or sensor range
sensors are not being used outside ofor returning a sentinel value due totemperature or sensor specifications. 4.)
internal malfunction or damage
Verify that the sensor is not physicallydamaged 5.) Call (800-446-7488) orcontact In-Situ Technical Support(support@in-situ.com).

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Navigating VuSitu
Program your Aqua TROLL® 600 with the VuSitu mobile app for Android or iOS. Or use Win-Situ 5 software
for a desktop PC.

VuSitu Mobile App
To avoid potential compatibility issues, it is important to use the most recent version of the
VuSitu Mobile App. Install the latest version from the Google Play Store or Apple App Store.
Connecting with Bluetooth
The Aqua TROLL 600 can connect to a Bluetooth-enabled device for wireless communication with the VuSitu
mobile app.
• If the Aqua TROLL 600 is connected to a Wireless TROLL Com, press the button on the Wireless TROLL
Com. Then open VuSitu to connect.
• To connect to the Aqua TROLL 600 directly, turn on the Aqua TROLL 600 LCD screen by holding thesonde vertical with the sensor end facing up. Then open VuSitu to connect.

Connected Instrument Screen

Access help
information.

Access menu.

Take single readings orcontinuously record at
two-second intervals.

Configure andaccess logs on the
Aqua TROLL 600.
Calibrate sensors.
Access instrument
clock and telemetry
settings.

Disconnect app
from instrument.

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VuSitu Menu Options
The features available in the VuSitu mobile app vary slightly depending on the instrument towhich it is connected.

Tap the menu icon in theupper left portion of thescreen to view options.
Tap the icon again toclose.

Some features aren’t
available when VuSitu
isn’t connected to an
instrument.

Selecting with Long-press and Swipe

Press and hold any item
in a list of files. You can
now select multiple files.

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Press and swipe left toreveal the delete andshare icons.

Press and swipe right toreveal the sharing icon.

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HydroVu
HydroVu is a data-management application that runs in the browser. Use it to manage data,
view graphs, and configure telemetry devices for remote monitoring. See it atwww.hydrovu.com.

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Live Readings in VuSitu
The live readings screen displays measurements taken from the instrument every two seconds.
You can save these readings and share them via email or cloud storage.

Snapshot Mode

Tap the button on thebottom left to toggle
between snapshot andlive readings modes.

Tap Change Locationin the top right corner
if you wish to associate
this data with a different
location.

VuSitu confirms the newsnapshot file.

View the file from theData Files screen.

Choose the desired
location and press Savein the bottom rightcorner of the screen.

Tap Save Single Reading
to create a snapshot.

Tap Stop to end therecording. VuSitu displays
a summary of the live
readings data.

Tap Save to if you wish toshare the Live Readings
file via email or cloudstorage.

Live Readings Mode

Tap the button on thebottom left to switchfrom snapshot mode tolive readings mode.

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Tap Start Recording.
The instrument takesa reading every two
seconds.

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VuSitu Data
Downloading Your Data
You can transfer a data file from your mobile device to a PC via Bluetooth, email it to yourself or
any valid email address, or upload it to Google Drive.

Pair VuSitu with the
instrument. SelectLogging from theConnected Instrument
screen.

Tap a log and press the
Download button.

Choose the data you wish VuSitu displays ato download. To save the progress bar while
entire log to your phone, downloading.
choose All data.

Tap and hold the nameof the log you want toshare.

Select Export.

Sharing Data

Select Data Files fromthe menu at the top left
corner of the screen.

Choose email, cloudstorage, or anothersharing option.

To save data locally on your mobile device, export to a third-party file management app.

Viewing Data on a Mac or PC
You’ll need to extract your files to view them. To do that on a Mac, double-click the Zip folder.
On a PC, right-click on the folder and choose Extract. Then open your files in Excel.

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VuSitu Locations
About VuSitu Locations
A VuSitu location represents the physical spot where an instrument collects data. You can create a VuSitu
location for any monitoring site. If you don’t create a location, your data defaults to “Device Location.”
Location names appear on the live readings screen, in snapshot files, and in log files.

How to Create a Location

Select Locations fromthe main menu.

Tap Add Location.

To home in on yourmobile device’s current
location, tap the buttonon the top right.

Tap the pin icon toestablish the location on
the map.

Enter a name for thelocation. You can also
add notes.

If desired, tap the camera
icon to take a photo of
the new location.

As an alternative, you can manually enter latitude and longitude values and tap Apply. Or, tapand hold a specific point on the map to drop a pin there.

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How to Select a Location
Data is associated with the Location that is displayed on the Live readings screen. After you
have created a Location, you must select it in order for your data to be associated with the
Location.

HQ
Fort Collins, CO

Select Locations fromthe app menu.

Tap a location to select it. New live readings data
will be associated withthis location until you
select another.

How to Edit or Delete a Location

Select Locations fromthe app menu.

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Tap the location you
wish to delete and swipe
left. Tap the trash icon.

Confirm by tapping
Delete.

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Logging
How to Create a Log with VuSitu...
Connect to the instrument via VuSitu to create a log. Then follow the on-screen instructions.
You can choose a start time for the log or start the log with a button press.

1
Select Logging.

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2

3

Tap New Log.

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Follow the on-screen
instructions to create andschedule a log.

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Remote-Monitoring Configuration (Telemetry)
VuLink can provide power to the Aqua TROLL 600 and uploads data to the HydroVu Data server.

1 Remove the battery pull-tab.
Never mix old and new batteries, orLithium and alkaline batteries. Make
sure all three batteries are installed in
the same orientation.
Remove the battery
cover by twisting it
counter-clockwise andpulling down.

Remove the yellow pulltab to allow current
to flow through thebatteries.

2 Connect the instrument.
cclilicckk

Align the flat edge of theconnector with the flatedge inside the cable.

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Twist the cable until itclicks into the secure
postion.

Connect the cable toyour instrument.

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3 Press the button on the VuLink control panel.
Connecting to a cellular network can take up to 10 minutes in the following situations:• The first time VuLink powers up• When VuLink hasn’t been powered up in several weeks or months

Pressing the button
starts the instrument
connection process.

All LEDs turn green for
Next, the connection
one second. The battery status LED and Bluetooth
status LED changes
status LED blink.
according to power level.

Once linked to an
instrument, VuLink
attempts to connect to adata network.

When the modem status
LED turns green, VuLink
begins connecting toHydroVu. The data LED
blinks.

The connection status
LED turns solid green,
indicating VuLink
is connected to an
instrument.

All LEDs except theBluetooth statusindicator turn green.

By default, VuLink reads all available parameters every hour and transmits to the cloud once
daily. You can also connect to the VuLink using the VuSitu Mobile App to configure a custom
log. See the VuLink manual for more infomation.

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Connecting to Win-Situ on a PC
Connecting to Win-Situ via USB
Download and install Win-Situ by visiting www.in-situ.com. The Wireless TROLL Com must beconnected to the sonde and powered on to connect the instrument to Win-Situ.
You can connect a Wireless TROLL Com to a PC with the included USB cable. Plug the cable into
the port at the top of the TROLL Com and the USB port on your PC.

To PC USB port

Open Win-Situ. Select No
when asked to connect
now.

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Click Preferences > Com
Settings from menu
bar and choose correctcom port. Select Serial
Communications button.
Click check mark button.

Click the yellow connect
button at the bottom
right of the screen.

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Click Yes if prompted tosync device time withlocal system.

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Navigating the Win-Situ Interface
Home Tab
View data files

Display Sites
list

Meter view

List view

Graph view

Recordsnapshot

Recordcurrent
valuesSensor Tab

Set up sensor(double-click)

Calibrate
sensor
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Device Setup Tab

Logging Tab

Log Status Indicators
Ready
Pending
Running
Suspended
StoppedCreateNew Log
Edit Pending DeleteLog
Log

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Deleted

Start
Log

Restart
Log

Stop
Log

63

DownloadLog to PC

Invalid

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BaroMerge
Using BaroMerge Software
BaroMerge Software is used to post-correct absolute (non-vented) level sensor data to eliminate barometricpressure effects from the measurements. BaroMerge Software can be accessed through the Win-Situ 5
Software Tools menu. BaroMerge provides three options to correct data.
• Fixed Correction—A single offset value is applied to all selected log data. Use this option if you know thebarometric pressure of the site during the log, and know that it did not change.
• Manual Entry—Specify two or more correction values to apply to the log data. Use this option if you wish
to manually enter a data set of barometric pressure values.
• BaroTROLL log file—Absolute level sensor data points are individually corrected to reflect barometricpressure changes that were logged by a BaroTROLL instrument during the approximate time period.

Baro Merge Input—Manual Entry
When you select the Fixed Correction and Manual Entry options, it is important to know the barometricpressure for the general time period covered by the log or logs you want to correct.
1 From the Tools menu, select Win-Situ BaroMerge.

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1 Select the Enter one or more values manually option.
2 The compensation table appears that allows you to build a table of barometric data that corresponds tothe time the log was recorded. The compensation table has three preference options:• The first option, Save calculated barometric adjustments in the new data file(s) is the default option. It
adds additional columns depending on which parameters were selected for the absolute/non-vented log
in the corrected BaroMerge file that uses the compensation table values. This is intended to show how
the adjustments were done in the BaroMerge file. If you do not want to show these adjustments, clear thisoption.
• When the second option, Show time in UTC is selected, the compensation table time stamp displays in
Coordinated Universal Time (UTC) time, formally known as Greenwich Mean Time (GMT).
• If the third option, Ignore daylight saving time (DST) is selected, the compensation table time stamp
format without the daylight savings time adjustment will be shown.
3 Build a table that contains at least two barometric pressure values.
4 Click the right arrow and select the absolute (non-vented) log file or files you intend to correct.
5 Click the check mark and the barometric compensation is applied.
6 Compensated data files can be viewed or exported from the Data tab.
Baro Merge Input—Fixed Correction
If you select the Apply a fixed correction option, a single correction is applied to all values in the log.
To use this correction method you need barometric pressure values from a reliable source. Choose a single
value that represents the actual ambient barometric pressure during the time period the log was recorded.
You also need to know the file names of the logs you want to correct.

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1 From the Tools menu, select Win-Situ Baro Merge.

2 Select the Apply a fixed correction option.
3 Enter the barometric correction value and select units from the drop-down menu.
4 Click the right arrow button.
5 Select the log files to which the correction will be applied and click the check mark button.
6 Compensated data files can be viewed or exported from the Data tab.
BaroMerge Input—BaroTROLL FileLog files that contain absolute data can be barometrically compensated using values logged by the InSitu Inc. BaroTROLL Instrument. Select this method when you have access to a BaroTROLL log file covering
approximately the same time period as the data file you intend to correct.
To use this correction method, you need the name of the BaroTROLL log file and the name(s) of the absolutelog file(s) you want to correct.

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1 From the Tools menu in Win-Situ 5 Software, select Win-Situ BaroMerge.

2 Select the “Use a BaroTROLL file:” option.
3 Click the browse button to the right of the file field.
4 Select a BaroTROLL file and click the check mark.
5 Values from the BaroTROLL file will be displayed in the next window. You can edit these values ifnecessary.
6 Click the right arrow button.
7 Select the log file(s) you intend to correct and click the check mark.
8 Compensated data files can be viewed or exported from the Data tab.
BaroMerge Output
Your original log file is not changed. A new, corrected log file with the same name and path is created. The
original “.wsl” extension is replaced by “-Baro Merge.wsl”.
Post Level Correction within Baro Merge
It is also possible to post correct a level reference in data collected from an absolute sensor if the data has
been compensated through Baro Merge Software.
1 Follow the steps to compensate the absolute data for barometric pressure.
2 When you reach this screen in the Wizard, you can select the check box to Post correct files for level.

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Connecting the Aqua TROLL 600 to a PLC or Data Logger
SDI-12 3 Wire

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Modbus Master RS485

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Modbus Master with RS232 (Converter Required)

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Modbus PLC Interface
Overview
The Modbus PLC Interface is a simplified method of communicating with the Aqua TROLL 600 using the
Modbus protocol. It reduces programming complexity and allows the user to remove sensors and reinstallthem in different ports. Please observe the following limitations when using this interface:• Only one sensor of any sensor model can be used in the sonde (for example: only one turbidity sensor
can be installed).
• If a parameter is provided by more than one of the installed sensors, the interface will return the firstvalue available.
• Firmware version 1.71 or later must be installed on the sonde.
For information about the full Modbus capabilities of your sonde, see the Aqua TROLL 500/600 Interface
Specification at www.in-situ.com/support/type/documentation.

Setting Up Instrument
1. Install the sensors and turn on the display by holding the instrument vertically.
a. Ensure the display turns on and check the LCD to ensure the sensors are working.
2. The setup below is using the instrument's factory default settings. Use WinSitu or VuSitu to reset the
instrument tofactory defaults if they have been changed.
a. Take note of any changes in default units setup.

Wiring the Modbus Master
Connect the Twist-Lock termination to the instrument and wire the stripped-and-tinned connection asshown below:

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Programming the PLC
1. Setup the serial communication the following values:
Mode

Start Bit

Baud Rate

Data Bits

ParityStop Bit

RTU

1

19200

8

Even

1

2. Set the device address to: 1
3. Set the PLC to wake-up the device by sending any Modbus command.
a. This could be a carriage return, reading the slave id or reading any register.
4. Read the discovery register using Appendix A to trigger the instrument to scan the sensors.
a. The return value can be discarded.
b. Each register is a holding register. Some PLCs require you to add 40000 to the register number oraddress. For example: 9301 would be 49301.
c. Alternatively, you can prompt the instrument to discover its sensor mapping by connecting it to
the VuSitu mobile app or Win-Situ software.
5. Select the register to read on the PLC using Appendix B
a. Some PLC devices use the register number directly in programming statements, others use
registeraddresses, which are one less than the register number; the programmer must adhere to the PLC’s
programming styleb. Each register is a holding register. Some PLCs require you to add 40000 to the register number oraddress. For example: 5451 would be 45451.
6. Set the type of register to: 32-bit float
a. If asked by the PLC this is 2 registers
7. Set the byte order to: Big Endean (MSB)
a. This should be the default and may not be configurable on all PLCs

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Reading Parameters
To determine the starting register number for a given parameter register block, first determine its parameter
id by looking in the sensor’s parameter tables. Then calculate the starting register number of the parameter
block using the following equation.
Starting Register = (Parameter Id – 1) x 7 + 5451
For example, for the Conductivity Sensor, the parameter id for specific conductivity is 10 (bit 9 will be set inregister 6984 if it is available). The starting register number for the specific conductivity register block is thus
(10 – 1) x 7 + 5451 = 5514.
The starting register for each parameter points to a block of 7 registers that contain the following
information.
Register Offset

Size(Registers)

Mode & Access Level(R/W)

Data Type

0

2

R

float

The measured value
from sensor

2

1

R

ushortData Quality Id: If thisis 0 then there are noerrors or
warnings. See: Full
System Specification

3

1

R/W

float

Units Id for themeasured value. The
default values
are listed in the tablebelow.

4

1

R

ushortParameter Id: The ID of
the parameter for thislocation. See: Full
System Specification

5

2

R/W

float

Off line sentinelvalue: The value that’s
returned onerror or if the parameter
isn’t available. The
default
sentinel is 0.0

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Care and Maintenance
Maintenance Schedule
For best results, send the instrument to the manufacturer for factory calibration every 12 to 18
months.

User-Serviceable Parts

O-rings

Sensors

RDO Sensor Cap

Lubricate O-rings duringinitial setup. Check andreplace O-rings if worn,
damaged, or discolored.

See the sensor
instruction sheets fordetails about maintaining
and replacing.

Refer to the sensor cap
instruction sheet for
details.

Storage
Short-term (less than one week)

Remove the restrictor
and the end cap.

Put the restrictor on the
instrument in calibration
mode.

Pour 15 mL (5 oz) of clean Thread the restrictor
onto the sonde andwater into the restrictor
store.
and replace the cap.

Long-term (more than one week)

Remove the sensors andwiper.

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Dampen the sponge
inside the pH sensor
storage cap with
Storage Solution or pH 4
calibration standard.

Replace the caps at bothends of the sensor. Useelectrical tape to seal thestorage cap.

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Thread the restrictor
onto the sonde.

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Store the sonde and sensors at temperatures between -5º and 65º C.

Cleaning the Sonde
Rinse the sonde thoroughly, clean with warm water and mild soap, then rinse the sonde again. Allow to air
dry.
Prevent water from entering the cable connector.

Cleaning and Storing the pH/ORP Sensor
Routine MaintenanceIf the ORP platinum electrode is dull or dirty, it can be cleaned with a swab and methanol or isopropyl
alcohol. Rub the electrode gently until it is shiny.
The pH sensor must be kept moist for the life of the sensor.
The sensor fill solution has a shelf life of 2 years. Replace the fill solution every 5 to 6 months or when:
1 The sensor fails to calibrate within the acceptable slope and offset range.
2 Sensor readings vary.
3 Readings during calibration at pH 7 are greater than +30 mV or less than -30 mV.
4 Sensor is slow to respond.

If the sensor fails to calibrate after you replace the fill solution, replace the reference junction.
Replacing the Filling Solution

Remove sensor fromsonde and unscrewreference junction.

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Discard old solution.

Insert tube from filling
solution bottle into
sensor.

75

Squeeze solution into
reservoir until full. Slowly
remove tube.

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Reinstall reference
junction and wipe sensor
body dry.

Soak sensor in tap water
for at least 15 minutes.

If necessary, thoroughly clean the sensor connector to remove filling solution: Using adisposable pipette, fill the connector with isopropyl alcohol (70% to 100%), Shake to dry.
Repeat 3 times. Dry overnight. When thoroughly dry, calibrate the sensor.
Replacing the Junction
Replace the junction when the sensor fails to calibrate with a reasonable slope and offset, even after you
have replaced the filling solution.
1 Unscrew the reference junction and discard.
2 Replace the filling solution and screw in a new reference solution.
3 Soak for 15 minutes, then calibrate the sensor.

Keep the junction damp at all times to avoid a lengthy re-wetting process.
Cleaning
Begin with the gentlest cleaning method and continue to the others only if necessary. Do not directly wipe
the glass bulb. To clean the pH sensor, gently rinse with cold water. If further cleaning is required, consider
the nature of the debris.
To remove crystalline deposits:
1 Clean the sensor with warm water and mild soap.
2 Soak the sensor in 5% HCl solution for 10 to 30 minutes.
3 If deposits persist, alternate soaking in 5% HCl and 5% NaOH solutions.
To remove oily or greasy residue:
1 Clean the sensor with warm water and mild soap.
2 Methanol or isopropyl alcohol may be used for short soaking periods, up to 1 hour.
3 Do not soak the sensor in strong solvents, such as chlorinated solvents, ethers, or ketones, such asacetone.
To remove protein-like material, or slimy film:
1 Clean the sensor with warm water and mild soap.
2 Soak the sensor in 0.1 M HCl solution for 10 minutes and then rinse with deionized water.
After cleaning, rinse the sensor with water, and then soak overnight in pH 4 buffer.

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StorageDampen the sponge
inside the pH sensor
storage cap with
Storage Solution or pH 4
calibration standard.

Replace the caps at bothends of the sensor. Useelectrical tape to seal thestorage cap.

Do not store the pH sensor in DI water because it will deplete the reference solution anddrastically reduce the life of the sensor.

Cleaning and Storing the RDO Sensor
Routine Maintenance
1 Leave the sensor cap on.
2 Rinse the sensor with clean water.
3 Gently wipe with a soft cloth or brush if biofouling is present.
4 If extensive fouling or mineral buildup is present, soak the sensor in vinegar for 15 minutes, thensoak in deionized water for 15 minutes.

Do not use organic solvents—they will damage the sensor cap. Do not remove the sensor cap
when rinsing or brushing.
5 After cleaning the sensor, perform a 2-point calibration.
Cleaning the Optical Window
Clean the optical window only when changing the sensor cap.
1 Remove the cap.
2 Gently wipe the sensing window with the supplied lens cloth.

Do not wet the lens with any liquid.
Storage
Prior to installation, store the sensor body and cap in the factory supplied containers.
Once installed on the sonde, the RDO sensor can be stored wet or dry depending on the sensor
configuration of the sonde.
Never store the RDO sensor without the sensor cap once it has been installed on the sonde.

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Cleaning and Storing the Conductivity Sensor
Soaking the sensor in vinegar for longer than one hour can cause serious damage.
Begin with the most gentle cleaning method and continue to the other methods only if necessary.
To clean the conductivity sensor face, gently rinse with clean, cold water. If further cleaning is required,consider the nature of the debris.
To remove crystalline deposits:
1 Clean the sensor face with warm water and mild soap.
2 Use a soft brush to gently clean the sensor pins and temperature button. Ensure removal of all debris
around the base of the pins and button.
3 If crystalline deposits persist, soak in 5% HCl for 10 to 30 minutes followed by warm soapy water and softbrushing.
4 If deposits persist, alternate soaking in 5% HCl and 5% NaOH solutions followed by warm soapy waterand soft brushing.
To remove oily or greasy residue:•

Clean the sensor face with warm water and mild soap.

• Using a soft brush, gently clean the sensor pins and temperature button. Ensure removal of all residue
around the base of the pins and temperature button.
•

Isopropyl alcohol may be used for short soaking periods, up to one hour.

• Do not soak in strong solvents such as chlorinated solvents, ethers or ketones (such as acetone).
To remove protein-like material, or slimy film:• Clean the sensor face with warm water and mild soap.
• Using a soft brush, gently clean the sensor pins and temperature button. Ensure removal of all material/
film around the base of the pins and temperature button.
•

Soak the sensor in 0.10% HCl for 10 minutes and then rinse thoroughly with distilled water.

Storage
Prior to installation, store the sensor in the factory supplied container.
Once installed on the sonde, the Temperature Sensor and Conductivity Sensor can be stored wet or dry
depending on the sensor configuration of the sonde. For the best accuracy over instrument life, keep the
conductivity cell submersed in water for 24-48 hours prior to calibration and deployment.

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Cleaning and Storing the Turbidity Sensor
Routine Maintenance
The optical windows should be clear of foreign material. To clear material gently rub the sensing windows
using clean water and a soft cloth or swab. Do not use solvents on the sensor.
Storage
Prior to installation, store the sensor in the factory supplied container. Once installed on the sonde, theturbidity sensor can be stored wet or dry depending on the sensor configuration of the sonde.
Replacing Wiper Bristles
Wiper bristles need to be replaced based on site conditions. In-Situ recommends replacing bristles at least
every 12 months or when visibly bent, damaged, or fouled.

Replace the Bristles
1 Slide both bristle sets out of the wiper head.
2 Insert new small bristle set into the small slot and the large bristle set in the large slot.
Replace the Entire Brush
1 Loosen the set screw near the base of the wiper head.
2 Slide the wiper head off the wiper shaft.
3 Slide the wipe head on to the wiper shaft, and position the wiper head so the set screw faces the flat partof the wiper shaft.

4 Tighten the set screw near the base of the wiper head.
Cleaning the Copper Antifouling Restrictor
When copper is deployed in environmental waters, particularly marine environments, the copper willoxidize and its biocidal properties decrease. Cleaning the copper restores the effectiveness of therestrictor’s antifouling capability.
1 Remove the restrictor from the sonde.
2 Remove the restrictor end cap.
3 Submerge the restrictor in white vinegar (acetic acid) for 24 hours.
4 Rinse the restrictor in water and air dry.

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More Information
To learn more about the Aqua TROLL 600, telemetry, software and other In-Situ products, see the resources listed
below.

1

Visit www.in-situ.com
Find information about In-Situ water quality, water level, telemetry and other products. Download
software, manuals and product instructions.

2View the In-Situ YouTube channel.
Get video instructions for the Aqua TROLL 600 and other instruments. Watch quickstart videos andother tutorials.

3

Call In-Situ's technical support team.
For further instructions and help with technical questions, call the In-Situ support line at
1-970-498-100.

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Declaration of Conformity

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Appendix
Appendix A: Parameter Discovery
The first register read in a PLC measurement sequence should be a 14-register block beginning with register
number 6984. A read of these registers triggers the sonde to scan its sensor ports and update its sensor
map. This guarantees that the sonde has properly registered any changes to the sensor configuration auser may have made since the last measurement sequence. The bitwise contents of these registers indicate
which parameter IDs (1 to 219) are currently available from the sonde according to the table below. Refer toAppendix B for a description of parameter IDs.
Parameter ID Map
Register

Bit
15

14

13...2

1

0

6984

16

15

14...3

2

1

6985

32

31

30...19

18

17

6986

48

47

46...35

33

33

6987

64

63

62...51

49

49

6988

80

79

78...67

65

65

6989

96

95

94...83

81

81

6990

112

111

110...99

97

97

6991

128

127

126.115

113

113

6992

144

143

142...131

129

129

6993

160

159

158...147

145

145

6994

176

175

174...163

161

161

6995

192

191

190...179

177

177

6996

208

207

206...195

193

193

6997

0

0

219...211

210

209

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Appendix B: Parameter Numbers and Locations
Holding Register
NumberHolding Register
Address

Default Units

Temperature

5451

5450

1 = °C

2

Pressure

5458

5457

17 = PSI

3

Depth

5465

5464

38 = feet

5472

5471

38 = feet

5479

5478

38 = feetID

Parameter Name

1

4
5

Level, Depth to
Water
Level, Surface
Elevation

9

Actual Conductivity

5507

5506

65 = µS/cm

10Specific
Conductivity

5514

5513

65 = µS/cm

11

Resistivity

5521

5520

81 = ohm-cm

12

Salinity

5528

5527

97 = PSU

13

Total Dissolved
Solids5535

5534

114 = ppt

14

Density of Water

5542

5541

129 = g/cm3

16

Barometric Pressure

5556

5555

22 = mmHg

17

pH

5563

5562

145 = pH

18

pH mV

5570

5569

162 = mV

19

ORP

5577

5576

162 = mV

5584

5583

117 = mg/L

5591

5590

177 = % Saturation

20
21

Dissolved Oxygen
Concentration
Dissolved Oxygen% Saturation

24

Chloride (Cl-)

5612

5611

117 = mg/L

25

Turbidity

5619

5618

194 = NTU

5654

5653

26 = torr

5661

5660

117 = mg/L

30
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Oxygen Partial
Pressure
Total Suspended
Solids83

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ID

Parameter Name

Holding
Register
Number32

External Voltage

33

Holding Register
Address

Default Units

5668

5667

163 = Volts

Battery Capacity (remaining)

5675

5674

241 = %

34

Rhodamine WT Concentration

5682

5681

118 = µg/L

35

Rhodamine WT Fluorescence
Intensity

5689

5688

257 = RFU

36

Chloride (Cl-) mV

5696

5695

162 = mV

37

Nitrate as Nitrogen (NO3--N)
concentration

5703

5702

117 = mg/L

38

Nitrate (NO3-) mV

5710

5709

162 = mV

39

Ammonium as Nitrogen(NH4+-N) concentration

5717

5716

117 = mg/L

40

Ammonium (NH4) mV

5724

5723

162 = mV

5731

5730

117 = mg/L

5738

5737

117 = mg/L

41
42

Ammonia as Nitrogen (NH3-N)concentration
Total Ammonia as Nitrogen(NH3-N) concentration

48

Eh

5780

5779

162 = mV

49

Velocity

5787

5786

118 = µg/L

50

Chlorophyll-a Concentration

5794

5793

118 = µg/L

5801

5800

257 = RFU

5822

5821

118 = µg/L

5829

5828

257 = RFU

5850

5849

118 = µg/L

5857

5856

257 = RFU

51
54
55
58
59

Chlorophyll-a Fluorescence
Intensity
Blue Green Algae-Phycocyanin
Concentration
Blue Green Algae-Phycocyanin
Fluorescence Intensity
Blue Green AlgaePhycoerythrin Concentration
Blue Green AlgaePhycoerythrin Fluorescence
Intensity

67

Fluorescein WT Concentration

5913

5912

118 = µg/L

68

Fluorescein WT Fluorescence
Intensity

5920

5919

257 = RFU

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ID
69
70
80
81
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Holding
Register
NumberParameter Name
Fluorescent Dissolved Organic
Matter Concentration
Fluorescent Dissolved Organic
Matter Fluorescence Intensity
Crude Oil Concentration
Crude Oil Fluorescence
Intensity
Colored Dissolved Organic
Matter Concentration

85

Holding Register
Address

Default Units

5927

5926

118 = µg/L

5934

5933

257 = RFU

6004

6003

118 = µg/L

6011

6010

257 = RFU

6053

6052

118 = µg/L

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Appendix C: Unit IDs
ID

Abbreviation

Units

1

C

Celsius

2

F

Fahrenheit

3K

KelvinPressure, Barometric Pressure (17-32)

17

PSI

Pounds per square inch

18

Pa

Pascals

19

kPa

Kilopascals

20

Bar

Bars

21

mBar

Millibars

22

mmHg

Millimeters of Mercury (0 to C)

23

inHg

Inches of Mercury (4 to C)

24

cmH2O

Centimeters of water (4 to C)

25

inH2O

Inches of water (4 to C)

26

Torr

Torr

27

atm

Standard atmosphere
Distance/Length (33-48)

33mm

Millimeters

34cm

Centimeters

35m

Meters36km

Kilometer

37

inInches

38

ft

Feet
Coordinates (49-64)

49

deg

Degrees50

min

Minutes51

sec

Seconds

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Conductivity (65-80)
65

µS/cm

Microsiemens per centimeter

66

mS/cm

Millisiemens per centimeter
Resistivity (81-96)

81

ohm-cmOhm-centimeters
Salinity (97-112)

97

PSU

Practical Salinity Units

98

ppt

Parts per thousand salinity
Concentration

113

ppm

Parts per million

114

ppt

Parts per thousand

115(Available)

116(Available)

117

mg/L

Milligrams per liter

118

µg/L

Micrograms per liter

119

---

(Deprecated)

120

g/L

Grams per liter

121

ppb

Parts per billion
Density (129-144)

129

g/cm3

Grams per cubic centimeterpH (145-160)

145

pH

pH
Voltage (161-176)

161

µV

Microvolts

162

mV

Millivolts

163

V

Volts
Dissolved Oxygen (DO) % Saturation (177-192)

177

% sat

Percent saturation
Turbidity (193-208)

193

FNU

Formazin nephelometric units

194

NTU

Nephelometric turbidity units

195

FTU

Formazin turbidity units

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Flow (209-224)
209

ft3/s

Cubic feet per second

210(Available - was Cubic feet per minute)

211(Available - was Cubic feet per hour)

212

ft3/day

Cubic feet per day

213

gal/s

Gallons per second

214

gal/min

Gallons per minute215

gal/hr

Gallons per hour

216

MGD

Millions of gallons per day

217

m3/secCubic meters per second

218
219

(Available - was Cubic meters per minute)
m3/hr

220

Cubic meters per hour
(Available - was Cubic meters per day)

221

L/s

Liters per second

222

ML/dayMillions of liters per day

223

mL/minMilliliters per minute224

kL/dayThousands of liters per day
Volume (225-240)

225

ft3

Cubic feet

226

gal

Gallons

227

Mgal

Millions of gallons

228

m3

Cubic meters

229

L

Liters230

acre-ft

Acre feet

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231

mL

Milliliters

232

ML

Millions of liters

233

kL

Thousands of liters

234

Acre-in

Acre inches% (241-256)

241%

Percent
Fluorescence (257-2720

257

RFU

Relative Fluorescence Units
Low-Flow (273-288)

273

mL/secMilliliters per second

274

mL/hr

Milliliters per hour

275

L/min

Liters per minute

276

L/hr

Liters per hour
Current (289-304)

289

µA

Microamps

290

mA

Milliamps

291

A

Amps
Velocity (305-320)

305

ft/s

Feet per second

306

m/s

Meters per second

Appendix D: Register Data Formats
The Modbus protocol specification requires any multiple-byte data type to be transmitted in
Big Endean order, or most significant byte (MSB) first. In-Situ devices shall use the following
register data formats.
Register Data Type Table
ID

Type

Size
(registers)

Description

2

Unsigned Short1

2 bytes, 1 register, MSB first

5

Float

2

4 bytes, 2 registers, IEEE floating point format

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