BD LSRFortessa™ X-20 Cell Analyzer
Special Order Product
Filter Guide*4 LaserDyes

Standard Filters

Standard Mirrors

355 nm
BD Horizon Brilliant™ Ultraviolet 395

379/28NABD Horizon Brilliant™ Ultraviolet 737

740/35690 LP

405 nm
BD Horizon Brilliant™ Violet 421, BD Horizon™ V450

450/50NA

BD Horizon Brilliant™ Violet 510, BD Horizon™ V500

525/50505 LP

BD Horizon Brilliant™ Violet 605

610/20595 LP

BD Horizon Brilliant™ Violet 650

670/30655 LP

BD Horizon Brilliant™ Violet 711

710/50690 LP

BD Horizon Brilliant™ Violet 786

780/60750 LPFITC, BD Horizon Brilliant™ Blue 515, Alexa Fluor® 488

530/30505 LPPE, Propidium Iodide (PI)575/26550 LP

BD Horizon™ PE-CF594

610/20595 LP

7-AAD, PE-Cy™5, PerCP, PerCP-Cy™5.5

670/30655 LP

PE-Cy™7780/60

750 LP

APC, Alexa Fluor® 647

670/30

NAAlexa Fluor® 700

730/45710 LP

APC-H7, APC-Cy7780/60

750 LP

DAPI, Hoechst, Indo-1 (violet)

450/50NA†

Indo-1 (blue)

530/30505 LP

488 nm

640 nmAdditional UV (355-nm) filters:*Commonly used filter configurations recommended by BD Biosciences. Other application-specific configurations with other lasers available through the special order program.
†Assumes the 450/50 BP filter is in the last PMT position. A 410-420 LP mirror can be used if the 450/50 BP filter is in any other position.
LP = Longpass Filter

BD LSRFortessa™ X-20 Cell Analyzer
Special Order Product
Filter Guide*
5 Laser
Dyes

Standard Filters

Standard Mirrors

355 nm
BD Horizon Brilliant Ultraviolet 395

379/28NABD Horizon Brilliant Ultraviolet 737

740/35690 LP

405 nm
BD Horizon Brilliant Violet 421, BD Horizon V450

450/50NABD Horizon Brilliant Violet 510, BD Horizon V500

525/50505 LP

BD Horizon Brilliant Violet 605

610/20595 LP

BD Horizon Brilliant Violet 650

670/30655 LP

BD Horizon Brilliant Violet 711

710/50690 LP

BD Horizon Brilliant Violet 786

780/60750 LP

FITC, BD Horizon Brilliant Blue 515, Alexa Fluor® 488

530/30505 LP

7-AAD, PerCP, PerCP-Cy5.5

670/30655 LPPE, Propidium Iodide (PI)586/15550 LP

BD Horizon PE-CF594

610/20595 LP

PE-Cy5670/30

635 LP

PE-Cy5.5710/50

685LP

PE-Cy7780/60

750 LP

488 nm561 nm

640 nm
APC, Alexa Fluor® 647670/30

NAAlexa Fluor® 700730/45

710 LP

APC-H7, APC-Cy7780/60

750 LP

DAPI, Hoechst, Indo-1 (violet)

450/50NA†Indo-1 (blue)

530/30505 LP

Additional UV (355-nm) filters:*Commonly used filter configurations recommended by BD Biosciences. Other application-specific configurations with other lasers available through the special order program.
†Assumes the 450/50 BP filter is in the last PMT position. A 410-420 LP mirror can be used if the 450/50 BP filter is in any other position.
LP = Longpass Filter

Class 1 Laser Product.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
APC-Cy7: US Patent 5,714,386
Alexa Fluor® is a registered trademark of Life Technologies Corporation.
CF is a trademark of Biotium, Inc.
Cy™ is a trademark of GE Healthcare. Cy dyes are subject to proprietary rights of GE Healthcare and Carnegie Mellon University and are madeand sold under license from GE Healthcare only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from GE
Healthcare, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.
BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2014 BD
23-15312-02

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