BD LSRFortessa™ X-20 Cell Analyzer
Technical Specifications

The BD LSRFortessa™ X-20 cell analyzer puts the power ofthe BD LSR platform into a compact footprint. It can easily fit on
the benchtop for more cost-effective space utilization.
The instrument delivers the optimal sensitivity and resolution
required for multicolor applications. The BD LSRFortessa X-20 cellanalyzer can be used to detect up to 18 colors simultaneously andsupports up to 5 lasers. In addition to the reduced size, design
innovations make filters and detectors more accessible for easier
setup of new experiments.
Through the BD special order program, customers can choose
from 22 different wavelengths and a wide range of power
options. This flexibility allows users to configure the instrument tomeet their exact requirements for advanced assay development.

OpticsPerformance

Fluidics

Excitation Optics

Fluorescence Sensitivity

Sample Flow Rates

Excitation Optical Platform

FITC: 80 molecules of equivalent soluble
fluorochrome (MESF-FITC)

Front button panel provides three modes: RUN,STANDBY, and PRIME

PE: 30 molecules of equivalent soluble fluorochrome(MESF-PE)

Continuously adjustable flow rate, plus three preset
flow rates:

PE-Cy™5: 10 molecules of equivalent soluble
fluorochrome (MESF-PE Cy5)

LO: 12 μL/min

APC: 70 molecules of equivalent soluble
fluorochrome (MESF-APC)

HI: 60 μL/min

The BD LSRFortessa X-20 optical layout allows for up
to five lasers.*

Laser Power355 nm: 15 mW
405 nm: 50 mW
488 nm: 50 mW
561 nm: 50 mW
640 nm: 40 mW

Optical Efficiency
Power loss at flow cell: <20% of specified laser
power

Flow Cell Design
Rectangular quartz cuvette:Internal cross-section,430 x 180 μm
External quartz cuvette surfaces are anti-reflective
coated for optimal transmission of laser light.
Fixed optical assembly with spatially separated laserbeams.

Emission Optics
Optical Coupling
The quartz cuvette flow cell is gel-coupled byrefractive index-matching optical gel to thefluorescence objective lens (1.2 NA) for optimalcollection efficiency.

FITC and PE measurements performed using
SPHERO™ Rainbow Calibration Particles (RCP-30-5A)
PE-Cy5 and APC measurements performed using
SPHERO™ Ultra Rainbow Calibration Particles (URCP38-2K)

Fluorescence Resolution
Coefficient of variation PI: Area of <3%, full
G0/G1 peak for propidium iodide (PI)-stained chicken
erythrocyte nuclei (CEN)

Fluorescence Linearity
Doublet/singlet ratio of 1.95–2.05 for CEN stainedwith PI and excited with the 488-nm blue laser

Forward and Side Scatter Sensitivity
Enables separation of fixed platelets from noise.

Forward and Side Scatter Resolution
Scatter performance is optimized for resolving
lymphocytes, monocytes, and granulocytes.

Forward Scatter Detection

Side Scatter Resolution

Photodiode detector with a 488/10 bandpass (BP)filterEnables separation of 0.5-μm beads from noise.

Forward Scatter PMT Option

Side Scatter Detector

A forward scatter PMT upgrade is available for small
particle detection. Requires the 100-mW, 488-nm
blue laser.

Photomultiplier tube (PMT) with a 488/10 BP filter

Emission Optical Design
Emitted light from the gel-coupled cuvette isdelivered by fiber optics to the detector arrays.
The BD LSRFortessa X-20 uses configurable polygonshaped optical pathways that use signal reflection tomaximize signal detection. Please see the filter guideat bdbiosciences.com for information on dye andfilter options.

Data Acquisition Rate
40,000 events/sec with beads

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

MED: 35 μL/min

Standard Fluidic Reservoirs
One 8-L sheath container and one 10-L waste
container provided.

Recommended Fluidics Option
BD FACSFlow™ supply system: automated fluidics
system, which includes a rolling cart and two 20-L
Cubitainer® packages

BD LSRFortessa™ X-20 Cell Analyzer
Technical Specifications
Data Management

Installation Requirements

Software: BD FACSDiva™ v8.0 or laterDimensions (H x W x D)
76.2 x 73.7 x 76.2 cm (30 x 29 x 30 in.)Workstation†

Operating System

Weight~159 kg (~350 lb) depending on configuration

Temperature Operating Range

Microsoft® Windows® 7 Professional 32-bit OS SP1

66ºF–79ºF (19ºC–26ºC)

Processor

HumidityIntel® Xeon® E3-1240v3 3.4 GHz CPU

10% to 90% relative, non-condensing

RAM

Heat Dissipation

4 GB

Compliance with Safety
Standards
UL 61010 (US)IEC 61010 and IEC 60825 (Europe)
CAN/CSA - C22.2 No. 61010 (Canada)~2,700 BTU per hour depending on configuration

PowerOperation at 100/120/230 VAC and 50 or 60 Hz

Options
Monitor Options
Two 19-in. LCDs, 2560 x 1024 resolution (standard)
One 23-in. LCD, 1680 x 1050 resolution (optional)

Maximum power: 1,500 watts

Noise<70 dB

Air Supply

One 24-in. LCD, 1920 x 1200 resolution (optional)

None required

Printer Options

Electrical Requirements

Options vary by location. Please check with your local
sales representative.

High Throughput Option
The BD™ High Throughput Sampler (HTS) option
is available to increase your lab productivity byacquiring samples from a 96- or 384-well microtiterplate.

BD requires one dedicated circuit for the cytometerand the computer system (including printer) witha dedicated AC source not shared with any otherequipment. The instrument will be powered from theline conditioner supplied by BD Biosciences.

The HTS can be front or side mounted on theBD LSRFortessa X-20.

HTS Throughput
Acquisition: Less than 15 minutes per microtiterplate in high-throughput mode using a 2-secondacquisition, less than 44 minutes in standard modeusing a 10-second acquisition

Carryover‡<0.5% High-throughput mode
<0.75% Standard mode*N ew laser options are available through the special orderprogram, and are developed on a regular basis. Pleasecheck with your local sales representative for the latest
wavelength and power options.
†Minimum configuration listed. Workstation may include
upgraded specifications.
‡High-throughput mode (HT) based on a 2-second sample
acquisition. Standard mode (STD) based on a 10-secondacquisition. Carryover based on testing performed withbeads and peripheral blood mononuclear cells. Other cell
types may require optimization of HTS parameters.

Class 1 Laser Product.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
SPHERO is a trademark of Spherotech, Inc.
Cubitainer is a registered trademark of Hedwin Corporation.
Microsoft and Windows are registered trademarks of Microsoft Corporation.
Intel and Xeon are registered trademarks of Intel Corporation.
NetXtreme is a registered trademark of Broadcom Corporation.
Cy™ is a trademark of GE Healthcare. Cy™ dyes are subject to proprietary rights of GE Healthcare and Carnegie Mellon University,and are made and sold under license from GE Healthcare only for research and in vitro diagnostic use. Any other use requires acommercial sublicense from GE Healthcare, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.
BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2015 BD
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