MCAM ™

KestrelA-K2850
Behavior

Ramonaʼs Multi-Camera Array Microscope™ (MCAM)
uses an array of micro-cameras to capture an entire wellplate in a single snapshot at 18K resolution.
The Kestrel Behavior System allows users tosimultaneously observe all wells of a standard well platewith 24X higher spatial and temporal resolution thanleading competitors. Detailed locomotion kinematics
and gross morphology are rapidly quantified as readouts
via customized high-speed software to unlock
completely new workflows for quantifying organism
behavior at high throughput.

Development Partners
High-throughput
Inspired by the tools scientists
use everyday, the Kestrel
Screening System is ideal forassessments in standard SBS
96-well plates.

A Uniform View
Perspective makes peering
into many well plates
problematic with a single lens.
Our array of micro-cameras
and wide-field illumination
ensure uniform observation
across all wells.

Speed Control
Select speeds from 20fps to340fps depending on thebehavioral question being asked— total movement vs escaperesponse, for example.

Custom Workﬂows
Already have an assay?
Integrate it easily in ourintuitive software and build
upon your lab expertise andour technology.

Kestrel
MCAM ™ Model A

OpticalCharacteristics

SensorCharacteristics

Data

Mechanical,Power, & Thermal

Field Of View
Live View | Stitched View

2850
Behavior

82mm x 118mm
Continuous | Live

Resolution

9.1µm/ pixel

Numerical Aperture

0.02

Working Distance

240mm

Depth of Field

3mm

Micro-Camera Array Size

6 x 4 = 24 micro-cameras

Micro-Camera Array Spacing

18mm

Micro-Camera Field of View

28.5mm x 28.5mm

Image Sensors

CMOS - RGB Color | Monochrome

Array Pixel Count

314 Megapixel

Pixel Size1.1µm

Bit Depth

8

Dark Current (Typ.)

5.9 LSB/s

SNR (Typ.)35 dB

Dynamic Range (Typ.)70.3 dB

Monochrome Peak QE

92% at 460 nm

RGB Color Peak QE

85% at 520 nm

Digital Gain

7.75

Analog Gain

2Minimum Exposure

1.5 microseconds

Maximum Exposure

9 seconds

Max. Frame Rate (full array)

22 fps (bin1)

Max. Frame Rate (partial array)

22 fps (bin 1)

Maximum Data Rate

45 Gb/sec | 19 Gb/sec

Data Transfer Interface

PCIe 3.0 (x16) | (x4)

Native File Format.nc (HDF5)

Exported Image File Format.tiﬀ, .bmp

Exported Video File Format.mp4

Export Options

By Well ID

Metadata.json

Metadata Options

By Plate Barcode

Local Storage

4 TB

Network-Attached Storage

Available Upon Request

Microscope Orientation

Upright | Inverted

AccessOpen | Enclosed

Vibration Dampening

Integrated

Dimensions (Typ.)

350mm x 350mm x 450mm

Weight (Typ.)

18kg

Stages - Motorized Linear Motion<3µm Repeatability

Multi-Camera Array
Microscope ™
System Field-of-View
Over 24 Multi-Well Plate= Well= Micro-Cam FOV= System FOV= Well Plate Perimeter

System Resolution: 9.1 µm per pixel<50µm Unidirectional Accuracy

Illumination

Stage Inserts

160mm x 110mm Universal Mount

Accepted Formats of Data by Well ID

Standard 24 & 96 Well Plates

Nominal Power Consumption

400W

Maximum Power Consumption

850W

Power

120V @ 60Hz | 240V @ 55Hz

Thermal Monitoring

K-Type Thermo. +/- 1.5C Accuracy | Reporting Integrated in Metadata

Active Thermal Control

Available Upon Request - Ramona + Tokai Hit Stage Top Module

Transmission

LED Structured Illumination Panel

Control

Individually Addressable (+1400 LEDs)

Spectra470 (B) + 518 (G) + 620 (R) + 840 (IR)

Reflection

LED Diﬀuse Illumination Rails

ControlGroup Digital AddressableSpectra

VIS | IR | UV

OperatingStandards

Ubuntu Linux

Version 22.04

PythonVersion 3.9

Operation

Acquisition Modes

Snapshot, Z-Stack, Timelapse, Video

Observation Modes

Brightfield, Darkfield, Fluorescence

Focus

Global Linear Z-PositioningGUI

Simple and Intuitive Application

API

Python-Based

Output Trigger

SMA Type @ 5V Signal

Image Capture Firmware

Custom Gigapixel Frame Grabber

Software &Firmware

Cross-Sensor Synchronization<6 microseconds

2D Image Mosaicing

Live

Gigapixel Image Stitching

Composite Array Frame Output (.tiﬀ)

Mosaic-Based 2D

Included

Neural Network-Based 2D

Available

High-Speed Video

Example: Parallelized tracking, 96 well-plate

Available

Bin260fps

Bin4

180fps; 340 fps (partial array)Control software

For MCAM Computer

Viewing Software

For Windows and Linux

Open API

docs.ramonaoptics.com

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