For patient-like
precision.

Mimix™ cell line-derived reference standards
For research use only. Not for use in diagnostic procedures.

Mimix cell line-derived reference standards

The importance of reference standards.
• The importance ofreference standards• Mimix reference standards• Ready-to-order formats• Liquid biopsy

Reference standards can provide confidence in results and help identify
and mitigate problems within your workflow by:• Checking that your assay is performing optimally• Determining limits of detection and minimal residual disease (MRD) status• Enhancing quality during assay development and manufacturing• Verifying end-to-end workflows with confidence• Pan-cancer• Benefiting from robust routine monitoring and ease of troubleshooting• Cancer-specificConfidence in workflow = Confidence in results

Did you know...
Reference standards are a material
with known and fixed parameters thatare used to provide a stable result toverify a successful experiment andto test for identity, concentration andquality of a particular substance.

• FFPE• gDNA• NIPT• Custom standards• Related products and services• Contact us

For research use only.
Not for use in diagnostic procedures.
2 |Mimix cell line-derived reference standards

Mimix reference standards• The importance ofreference standards• Mimix reference standards• Ready-to-order formats• Liquid biopsy• Pan-cancer• Cancer-specific• FFPE• gDNA• NIPT• Custom standards• Related products and services• Contact us

Revvity’s Mimix™ reference standards are cell line-derived to closely
mimic patient samples.
Unlike synthetic reference material, cell line-derived standards
maintain genomic complexity while mimicking patient material. They
are commutable, providing performance similar to a patient genome
sample during sequencing and analysis.
Mimix controls offer a reliable and renewable source of material,
whereas patient material is often limited in quantity. While FFPE patientmaterial can vary in quality and genetic profile between sections, Mimix
supports batch-to-batch consistency.

Genomic complexitiesof patient samples• Broad range of allelic frequencies tochallenge lab assays’ LoD instead ofjust hetero/homozygous variants• Copy number variants (CNVs)• Insertions and deletions (INDELs)• Fusions• Single nucleotide variants (SNVs)

Mimix reference standards are manufactured to ISO standards: ISO
9001:2015 and ISO 13485:2016. Customers can choose from a wide
range of off-the-shelf products, including multiple formats and allelicfrequencies for end-to-end process control. Options include single-plex,
multiplex, cancer-specific panels, and custom solutions available from
our in-house cell line engineering experts.

For research use only.
Not for use in diagnostic procedures.
3 |Mimix cell line-derived reference standards• The importance ofreference standards• Mimix reference standards• Ready-to-order formats• Liquid biopsy• Pan-cancer• Cancer-specific• FFPE• gDNA• NIPT• Custom standards

Controls forend-to-end workflows

Tumor biopsy

Our dedicated team of scientists
maintain and quality control celllines before and after blending tocreate high integrity, reliable andcustomizable reference material
in gDNA, cfDNA, or FFPE format.
Reference standards can beused at different stages of yourworkflow, giving you greater
confidence in your assay andresults.

DNA/RNA
extraction & QC

NGS/qPCR/ddPCR/
Microarray

Assay analysis

Bioinformatic control

Liquid biopsy

Assay control
DNA extraction quality control

Extraction control• Related products and services• Contact us

Different reference standard formats can act as a control for distinct stages of a workflow.

For research use only.
Not for use in diagnostic procedures.
4 |Mimix cell line-derived reference standards• The importance ofreference standards• Mimix reference standards• Ready-to-order formats• Liquid biopsy• Pan-cancer• Cancer-specific• FFPE

Cell line-derived
reference standards
Revvity’s Mimix reference
standards use a single cell line
or a blend of cell lines to create
products with mutations at
different allelic frequencies which
can also be customized.

DNA extraction• Custom standards

gDNA
standard

QC

Cell line 1
DNA extraction

QC

Cell line 2

QC
Blend 1DNA extraction

QC

DNA extraction

QC

Blend 2Cell line 3• gDNA• NIPT

DNA extraction

QC

Cell line 4...
A precise amountof each cell lines DNA• Related products and services• Contact usCharacterized cell lines containing specific variants are blended to create standards with a few or multiple variants of known allelicfrequencies. The blended cell mixture is then subjected to quality control analysis and processed to be available in a variety of formats.

For research use only.
Not for use in diagnostic procedures.
5 |Mimix cell line-derived reference standards

Off-the-shelf and ready-to-go!
• The importance ofreference standards• Mimix reference standards• Ready-to-order formats• Liquid biopsy• Pan-cancer• Cancer-specific• FFPE• gDNA• NIPT• Custom standards

Mimix is easy to order directly online - with instant quotes available. Plus, to help ensure yourworkflow is kept on track, orders can be shipped in as little as 48 hours.
Our products offer precise genetic variations and mutation allele frequencies, verified by
ddPCR and NGS.
The Mimix range has thousands of biologically relevant variants, available in a variety offormats to fit seamlessly into your workflow. These formats include FFPE, gDNA, cfDNA/
ctDNA, and formalin compromised options.
Browse our online catalog using specified formats to quickly find the Mimix reference standard
that meets your requirements.

Select your reference standard:
Liquid biopsy

Pan-cancer

Cancer-specific

NIPT
Order online
FFPE

gDNA

• Related products and services• Contact us

For research use only.
Not for use in diagnostic procedures.
6 |Mimix cell line-derived reference standards

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OffLiquid
the shelf
and
ready
to
go!
biopsy reference standards• The importance ofreference standards• Mimix reference standards• Ready-to-order formats• Liquid biopsy• Pan-cancer• Cancer-specific• FFPE• gDNA• NIPT• Custom standards• Related products and services

Ordering Mimix reference standards is easy via our website, with instantquotes available.
Andisproducts
beofshippedin as
as 48
Liquid biopsy
a growingcan
area
researchthatlittle
is keyfor hours,
theensuringearly
promptdeliverymeet yourneeds. of circulating tumor
detection
andtocontinual
monitoring
DNA (ctDNA).
Our products feature precise copy numbers and allele frequencies,
confirmed
by early
ddPCR,
ensuring
accuracy
reliability.
They are
Since
2015,
Revvityhas beenandproducingreference
also manufactured
toISO
standards,
holdingISO
9001:2015
and ISO
standards in the format of ctDNA for the surveillance of13485:2016
accreditations.
minimalresidual disease (MRD) tracked via liquid biopsy.
Revvity With
offersouroverbiologically
relevant
variants, available
in a variety
cell380
line-derived
referencestandards,
we provide:of formats to fit seamlessly into your workflow. These formats include• ctDNA reference standards to support the verification of
FFPE, gDNA, ctDNA, and formalin-compromised options.
liquid biopsy-based MRD assays
You can browse our online catalog according to specified formats, making• Matched negative controls for further dilution of allelicit easy to find the products that best suit your requirements.
frequency to suit your assay’s needs•

Material that mimics the biological complexity of apatient sample• Contact us

Liquid biopsy reference
standards

Read more

Revvity offers a wide range of Mimix ctDNA
cell line-derived reference standards, including:reference
Non-SmallPan-cancer
Cell Lung Cancer
Panel ctDNA
Read more
standards• Covers 22 mutations in EGFR, KRAS, BRAF and rare
mutations in genes including MET, ERBB, PIK3CA, NRAS
• Allelic frequencies as low as 0.3%
Cancer-specific
Prostate Cancer
Panel cfDNA reference standard
Read morereference standards• Multiplex multigene containing 17 variants across
9 genes biologically relevant to prostate cancer• Allelic frequencies of 0.3 - 10%
ColorectalNon-invasive
Cancer Panel ctDNA reference standard
Read moreprenatal testing (NIPT)• Detection of key actionable mutations in genes,
reference standards
including AKT1, APC, BRAF, KRAS, PIK3CA and TP53
• Allelic frequency as low as 1.25% and a negative
control available for further dilution
OncoSpan™
cfDNA
FFPE
reference standards

Read more• Pan-cancer panel containing over 380 variants across
152 genes

Browse all our liquid biopsy reference standards
gDNA reference standards

Read more

For research use only.
Not for use in diagnostic procedures.
7 |Mimix cell line-derived reference standards

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OffPan-cancer
the shelf reference
and readystandards
to go!
• The importance ofreference standards• Mimix reference standards• Ready-to-order formats• Liquid biopsy• Pan-cancer• Cancer-specific• FFPE• gDNA• NIPT• Custom standards• Related products and services• Contact us

Ordering Mimix reference standards is easy via our website, with instantquotes available.
Andaproducts
can ofbepan-cancer
shipped in reference
as little asstandards,
48 hours, including:Choose frombroad range
Liquid biopsy reference
ensuring prompt delivery to meet your needs.
standards
OncoSpan Reference Control Panel:
Our products
featurenumbers
andallele frequencies,• Oneof theprecise
world’scopy
largestoncologyreferencestandard with companion batch-specific in silico data set
confirmed by ddPCR, ensuring accuracy and reliability. They are• Contains 152 key genes for cancer screening
Pan-cancer reference
also manufactured to ISO standards, holding ISO 9001:2015 and ISO• Genes contain 380+ variants with a wide range of allelic frequencies (1-92.5%)
standards
13485:2016 accreditations.
• Available in formats cfDNA, FFPE and gDNA
Revvity offers over 380 biologically relevant variants, available in a variety
of formats
to fit seamlessly
your workflow.
Structural
Multiplex into
Reference
Standard:These formats include
Cancer-specific
FFPE, gDNA, ctDNA, and formalin-compromised options.
• 9 mutations of interest including RET and ROS1 fusion variants, MYC-N and MET focal
amplifications,referencestandards
and aourBRCA2variant
You can browse
onlinecatalog according to specified formats, making

Read more

Read more

Read more• findAvailable
in gDNA
andbestFFPE
it easy to
the products
thatsuit your requirements.
Non-invasive
prenatal testing (NIPT)
standards
Expression of TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET and reference
SLC34A2-ROS1

Pan-Cancer 6-Fusion:•

Read more

RNA transcripts confirmed by fusion specific RT-ddPCR using the QX200 ddPCR System (Bio-Rad)

Browse all our pan-cancer reference standards

FFPE reference standards

Read more

gDNA reference standards

Read more

For research use only.
Not for use in diagnostic procedures.
8 |Mimix cell line-derived reference standards

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OffCancer-specificthe shelf and reference
ready to go!
standards• The importance ofreference standards• Mimix reference standards• Ready-to-order formats• Liquid biopsy• Pan-cancer• Cancer-specific• FFPE• gDNA• NIPT

Ordering Mimix reference standards is easy via our website, with instantquotes available.
products
can berange
shippedin as cancer-specificlittle as 48 hours,
Our MimixAnd
referencestandard
includesproducts with disease-relevantin key oncogenes.
Liquidvariants
biopsy reference
Read moreensuringWe
promptdelivery tomeet yourstandards
are constantly
expandingourneeds.
offering of cancer-specific panels available off the shelf.
Highlights include:
Our products feature precise copy numbers and allele frequencies,
Non-SmallCellensuring
Lung Cancer
Panel
ctDNA:
confirmed
by ddPCR,
accuracyandreliability. They are• Supports
mutations inholding
Non-Small
Lung Cancer
also manufactured
toDNA
ISO standards,
ISO Cell
9001:2015
and ISO
which accounts for ~85% of all lung cancer cases
13485:2016(NSCLC)
accreditations.

•Pan-cancer
reference
Highly characterizedbiologically-relevantmaterial
Read more
standards
with somatic mutations• Covers 22 key and rare mutations• Relevant to breast and other cancers.
Revvity offers over 380 biologically relevant variants, available in a variety• BRCA1 and BRCA2 variants covered among 5 genes containingof formats to fit seamlessly into your workflow. These formats include
Cancer-specificMyeloid Cancer Panel cfDNA Reference Standard:a total of 16 mutations
at 7.5%-100% allelic frequency
Read more
FFPE, gDNA, ctDNA, and formalin-compromised options.
reference standards• 15 biologically-relevant variants across 14 genes
You can• browse
our online
catalog
accordingspecifiedmaking Prostate Cancer Panel cfDNA Reference Standard:
Available
negative
control
to dilute to
down
allelic formats,frequencies
it easy to find the products that best suit your requirements.
• Multiplex multigene panel containing 17 variants across 9 genes• Custom standards

Lymphoid Cancer Panel:• Related products and services•

10 cancer biomarkers key to tracking lymphoma•

Available in FFPE and gDNA

• Contact us

BRCA Somatic Multiplex (FFPE):

Non-invasive
biologically relevant
to prostate cancer•

prenatal testing (NIPT)
reference standards

Allelic frequencies of 0.3 - 10%

Read more

Colorectal Cancer Panel ctDNA :•For the second leading cancer in morality worldwide, thisRead more
standard is designed
to mimicstandards
the traces of mutations
FFPE reference
detectable in liquid biopsies for CRC

Browse all our cancer-specific reference standards
gDNA reference standards

Read more

For research use only.
Not for use in diagnostic procedures.
9 |Mimix cell line-derived reference standards

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OffFFPE
the reference
shelf andstandards
ready to go!
• The importance ofreference standards• Mimix reference standards• Ready-to-order formats• Liquid biopsy• Pan-cancer• Cancer-specific• FFPE• gDNA• NIPT• Custom standards• Related products and services• Contact us

Ordering Mimix reference standards is easy via our website, with instantRevvity’s Mimix range offers FFPE reference standards for testing workflows from DNA/RNA extraction all the way to analysis for endquotes available. And products can be shipped in as little as 48 hours,
Liquid biopsy reference
to-end control of potential variability in pre-analytical steps. They’re formalin fixed samples
in a paraffin block, and are available
Read moreasensuring prompt delivery to meet your needs.
standards
whole blocks, half blocks or sectioned into curls of 10 and 15uM.
Our products feature precise copy numbers and allele frequencies,
confirmed by ddPCR, ensuring accuracy and reliability. They are
PTEN Loss: to ISO standards, holding ISO 9001:2015 and ISO
also manufactured• The
first of its kind off-the-shelf in the market
13485:2016
accreditations.
• PTEN deletion is a prominent prognostic biomarker in prostate
Revvity offers over 380 biologically relevant variants, available in a variety
cancers as well as being reported in other pathologies like lung,of formats to fit seamlessly into your workflow. These formats include
ovary, endometrium, breast, and colon cancers.
FFPE, gDNA, ctDNA, and formalin-compromised options.
• Contains a cell-line with homozygous deletion (copy number loss)of PTEN
mimiccatalog
the lossaccordingof PTEN observedin several
cancers.
You can browse
ourtoonline
to specified
formats,
makingit easy to find the products that best suit your requirements.
Structural Multiplex Reference Standard:••••

Covering a wide range of mutations in a defined genomic context
9 biologically relevant variants across 8 genes.
Whole exome sequencing data with gDNA version available.
Available in a range of formats, including gDNA, cfDNA and FFPE.

Pan-Cancer 6-Fusion
Panel:
Pan-cancer
reference•••negative control available.
Quantitative Multiplex Moderate FFPE:•••

Browse all our FFPE reference standards

Read more

standards
Defined FFPE
control material containing 6 clinicallyrelevant, cell-based kinase fusion RNA biomarkers
Contains prevalent NTRK gene fusions as well as ALK,RET and ROS,
also suitable for NGS workflows.
Cancer-specific
Read more
Complimentary
Pan-Cancer
referencestandards6-Fusion Panel matched

Non-invasive

Consistent control material with patient-like Read moreprenatal testing (NIPT)
performancecontaining
11 highly onco-relevant
referencestandards
mutations
Moderately compromised FFPE, providing a version
more comparable to the degradation seen in realworld samples
Read more
FFPE reference standards
Covers multiple endogenous SNPs and deletions, in
multiple formats, including FFPE, gDNA and FormalinCompromised DNA
gDNA reference standards

Read more

For research use only.
Not for use in diagnostic procedures.
10 |Mimix cell line-derived reference standards

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OffgDNA
the shelf
and
ready
to
go!
reference standards• The importance ofreference standards• Mimix reference standards• Ready-to-order formats• Liquid biopsy• Pan-cancer• Cancer-specific• FFPE• gDNA• NIPT• Custom standards• Related products and services

Ordering Mimix reference standards is easy via our website, with instantquotes available.
And aproducts
bereference
shipped instandards:
as little as 48 hours,
Revvity offers
range of cangDNA
ensuring prompt delivery to meet your needs.
• Extracted DNA in Tris-EDTA buffer or synthetic plasma
Our products
featurecopyLoD
numbers
and allele frequencies,• Perfect
forprecise
establishingof assays
confirmed by ddPCR, ensuring accuracy and reliability. They are• Mimics patient’s solid tumor samples in sample processing
also manufactured to ISO standards, holding ISO 9001:2015 and ISO• Structural multiplex reference standard
13485:2016 accreditations.
- Covers a wide range of mutations in defined
Revvity offers genomic
over 380context,
biologically
relevant
including
RET variants,
and ROS1available
fusion in a variety
of formats to fit
seamlessly
into
your
workflow.
These formats include
variants,
MYC-N
and
MET
focal amplifications,
FFPE, gDNA, ctDNA,
and formalin-compromised
options.
and a BRCA2
variant- Includes 9 ddPCR-verified mutations, with most
You can browse our online catalog according to specified formats, making
centered at 5% allelic frequency
it easy to find the products that best suit your requirements.
•

Available in a range of formats, including FFPE to
support you in verifying pre-analytical and analytical
portions on workflow.

Liquid biopsy reference

Formalin-compromisedDNA
standards•

Understand sample quality anddegradation with our staged formalincompromised standard material
Pan-cancer reference- 3 stagesof formalin compromised
standards
standards (severe, moderate andmild) for comparable results against
degraded patient samples
Cancer-specificreference standards

Read more

Read more

Read more

Browse all of our fcDNA reference standards

Non-invasive
prenatal testing (NIPT)
reference standards

Read more

FFPE reference standards

Read more

gDNA reference standards

Read more• Contact us
Browse all our gDNA reference standards

For research use only.
Not for use in diagnostic procedures.
11 |Mimix cell line-derived reference standards

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OffNIPT
thereference
shelf andstandards
ready to go!
• The importance ofreference standards• Mimix reference standards• Ready-to-order formats• Liquid biopsy• Pan-cancer• Cancer-specific• FFPE• gDNA• NIPT• Custom standards• Related products and services

Ordering Mimix reference standards is easy via our website, with instantquotes available.
Andprenatal
products
can beshippedin as little
as 48thehours,
Non-invasive
testing(NIPT)
is a method
in which
cfDNA isensuringanalyzed
prompt indeliveryto meetneeds.
maternal
bloodyourto detectfor chromosomal abnormalities.
Our products
featurecopy
numbersare:and allele frequencies,
Our Mimix
NIPTprecise
referencestandards
confirmed by ddPCR, ensuring accuracy and reliability. They are• Cell line-derived, matched maternal-fetal cfDNA in synthetic plasma
also manufactured to ISO standards, holding ISO 9001:2015 and ISO• Cover
all three of the most common trisomies: 21, 18, and 13.
13485:2016
accreditations.
• Rigorously tested using ddPCR and Revvity’s Vanadis™ NIPT platforms
Revvity offers over 380 biologically relevant variants, available in a variety- Reproducible at 40 ng/ml concentration, 170 bp sizeof formats to fit seamlessly into your workflow. These formats include
distribution and 10% fetal fraction
FFPE, gDNA, ctDNA, and formalin-compromised options.
- Euploid control: negative for trisomy 13, 18, 21 available as
You can browse
our online
catalog according to specified formats, making
baselinecomparative
it easy tofindtheproducts
thatbest suit
your requirements.
• qPCR, ddPCR and
Vanadiscompatible
These controls enable you to confidently test and monitor workflows
designed to detect chromosomal abnormalities in maternal blood samples.

Liquid biopsy reference
standards

Read more

Pan-cancer reference
standards

Read more

Cancer-specificreference standards

Read more

Non-invasive
prenatal testing (NIPT)
reference standards

Read more

FFPE reference standards

Read more

gDNA reference standards

Read more• Contact us

Browse all our NIPT reference standards

For research use only.
Not for use in diagnostic procedures.
12 |Mimix cell line-derived reference standards

Custom reference standards• The importance ofreference standards• Mimix reference standards• Ready-to-order formats• Liquid biopsy• Pan-cancer• Cancer-specific• FFPE• gDNA• NIPT• Custom standards• Related products and services• Contact us

Need something more specific? Revvity’s in-housecell line engineering team allows you to selectalmost any genetic variants of interest and create
standards bespoke to your needs. This enables you
to design your own reference standard tailored toyour oncology NGS diagnostics pipeline, available inbuffer or plasma
With millions of pre-engineered variants to choose
from and the capability to engineer custom variants
in-house, you have full flexibility. Our products
are 100% cell line-derived and adhere to ISO
13485:2016 and ISO 9001:2015 standards to createhigh integrity and reliable reference material.
Additionally, we can custom blend to createa bespoke reference standard with different
genes and mutation allelic frequencies to meetthe specific requirements of your research anddiagnostic applications.

Create your own reference standard

Custom reference standards questions? Email us.
For research use only.
Not for use in diagnostic procedures.
13 |Mimix cell line-derived reference standards

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OffCreate
the shelf
and
ready
to
go!
your own reference standard• The importance ofreference standards• Mimix reference standards• Ready-to-order formats• Liquid biopsy• Pan-cancer• Cancer-specific• FFPE• gDNA• NIPT• Custom standards• Related products and services

Ordering Mimix reference standards is easy via our website, with instantquotes available.
And products
canyourbe shippedin as little inas 48 hours,
Use our online
tool to build
custom reference
ensuring5 prompt
deliveryyourinstantly.
needs.
easy steps- and togetmeeta quoteOur products
Step 1:feature
Format:precise copy numbers and allele frequencies,
confirmed
ddPCR, ensuring accuracy and reliability. They are
• by
FFPE
also manufactured to ISO standards, holding ISO 9001:2015 and ISO• gDNA
13485:2016 accreditations.
• cfDNA in buffer or Synthetic Matrix/plasma
Revvity offers over 380 biologically relevant variants, available in a variety
Stepto2:fitVariants:
of formats
seamlessly into your workflow. These formats include•
Singleplex/Multiplex
FFPE, gDNA, ctDNA, and formalin-compromised options.
• Up to 4 variants incorporated yourself on the MTO builder. If
You can browse our online catalog according to specified formats, making
you’d like more you can contact us.
it easy to find the products that best suit your requirements.
• Search using gene name and change
Step 3: Allelic frequency:•

Allelic frequency as low as 0.1% possible

Liquid biopsy reference
standards

Read more

Pan-cancer reference
standards

Read more

Cancer-specificreference standards

Read more

Non-invasive
prenatal testing (NIPT)
reference standards

Read more

FFPE reference standards

Read more• Contact us

Step 4: Average fragment length
Step 5: Amount of product:•

From as little as 15ug or 1/2 block FFPE (300 sections at 15uM)

Try our online tool now

gDNA reference standards

Read more

For research use only.
Not for use in diagnostic procedures.
14 |Mimix cell line-derived reference standards

Explore other Revvity products and services• The importance ofreference standards• Mimix reference standards• Ready-to-order formats• Liquid biopsy• Pan-cancer

Fontus™ NGS workstation

BioQule™ NGS system

NEXTFLEX™ library prep kits

Sequencing services

Omics sample processing services

Cell line engineering services

LabChip™ GX Touch™ nucleic acid analyzer

chemagic™ 360 instrument

Bead mill & automated homogenizers• Cancer-specific• FFPE• gDNA• NIPT• Custom standards• Related products and services• Contact us

For research use only.
Not for use in diagnostic procedures.
15 |www.revvity.com

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Email us at orders@horizondiscovery.com

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Questions about custom reference standards?
Email us at uk-cam-customdx@horizondiscovery.com