MCAM ™

KestrelA-K0950
Screening

Ramonaʼs Multi-Camera Array Microscope™ (MCAM)
uses an array of micro-cameras to capture an entire wellplate at cellular resolution in seconds.
As Ramonaʼs fluorescence compatible higher-resolution
system, the Kestrel Screening System is designed forhigh-content screening. It provides capabilities forparallelized functional fluorescence and bright-fieldimaging of specimens like zebrafish embryos, zebrafish
larvae and organoid cultures. From fine-morphology andcardiac assessment, to fluorescence quantification andtime lapse imaging, acquire multiple readoutsthroughout development in one integrated system.

Development Partners
High Content Imaging
Inspired by the tools scientists
use everyday, the Kestrel
Screening System is ideal forassessments in standard SBS
96-well plates.

Multiplex to Reduce Bias
Use multiple cameras to assesstime-sensitive developmental
readouts without time bias.
Rapidly screen specimens forhealth. Save time in the labwith 24X workflow speed-up.

Cells in Motion
Rapidly measure blood flow,
immune cell movement, andcardiac parameterssimultaneously across 96 samples
at diﬀerent developmental
time-points

Custom WorkﬂowsAlready have an assay?
Integrate it easily in ourintuitive software and build
upon your lab expertise andour technology.

Kestrel
MCAM ™ Model A

OpticalCharacteristics

SensorCharacteristics

Data

Mechanical,Power, & Thermal

Field Of View
Live View | Stitched View

0950
Screening

74mm x 110mm
Segmented | 2x2 Scan

Resolution

3.0µm/ pixel

Numerical Aperture

0.053

Working Distance

90mm

Depth of Field

0.50mmMicro-Camera Array Size

6 x 4 = 24 micro-cameras

Micro-Camera Array Spacing

18mm

Micro-Camera Field of View

9.5mm x 9.5mm

Image SensorsCMOS - RGB Color | Monochrome

Array Pixel Count

314 Megapixel

Pixel Size

1.1µm

Bit Depth

8Dark Current (Typ.)

5.9 LSB/s

SNR (Typ.)35 dB

Dynamic Range (Typ.)70.3 dB

Monochrome Peak QE

92% at 460nm

RGB Color Peak QE

85% at 520nm

Digital Gain

7.75

Analog Gain

2Minimum Exposure

1.5 microseconds

Maximum Exposure

9 seconds

Max. Frame Rate (full array)

22fps

Max. Frame Rate (partial array)

22fps (no binning)

Maximum Data Rate

45 Gb/sec | 19 Gb/sec

Data Transfer Interface

PCIe 3.0 (x16) | (x4)

Native File Format.nc (HDF5)

Exported Image File Format.tiﬀ, .bmp

Exported Video File Format.mp4

Export Options

By Well ID

Metadata.json

Metadata Options

By Plate BarcodeLocal Storage

4 TB

Network-Attached Storage

Available Upon Request

Microscope Orientation

Upright | Inverted

AccessOpen | Enclosed

Vibration Dampening

Integrated

Dimensions (Typ.)

350mm x 350mm x 450mm

Weight (Typ.)

22kg

Stages - Motorized Linear Motion<3µm Repeatability

Multi-Camera Array
Microscope ™
System Field-of-View
Over 96 Multi-Well Plate= Well
= Micro-Cam FOV= Micro-Cam Scan Area= System FOV= Well Plate Perimeter

Resolution (3.0 µm/pixel) and Fluorescence

GFP<50µm Unidirectional Accuracy

Illumination

Stage Inserts

160mm x 110mm Universal Mount

Accepted Formats of Data by Well ID

96 Multi-Well Plate

Nominal Power Consumption

400W

Maximum Power Consumption

850W

Power

120V @ 60Hz | 240V @ 55Hz

Thermal Monitoring

K-Type Thermo. +/- 1.5C Accuracy | Reporting Integrated in Metadata

Active Thermal Control

Available Upon Request - Ramona + Tokai Hit Stage Top Module

Transmission

LED Structured Illumination PanelControl

Individually Addressable (+1400 LEDs)

Spectra470 (B) + 518 (G) + 620 (R) + 840 (IR)

Reflection

Group Digital AddressableSpectra

VIS | IR | UV

Fluorescence

OperatingStandards
Operation

Software &Firmware

LED Diﬀuse Illumination Rails

Control

GFP/ RFP/ BFP

Ubuntu Linux

Version 22.04

PythonVersion 3.9

Acquisition Modes

Snapshot, Z-Stack, Timelapse, Video

Observation Modes

Brightfield, Darkfield, Fluorescence

Focus

Global Linear Z-PositioningGUI

Simple and Intuitive Application

API

Python-Based

Output Trigger

SMA Type @ 5V Signal

Image Capture Firmware

Custom Gigapixel Frame Grabber

Cross-Sensor Synchronization<6 microseconds

2D Image Mosaicing

Live

X 24 per snapshot

X 24 per snapshot

Composite Array Frame Output (.tiﬀ)

Mosaic-Based 2D

Included

Neural Network-Based 2D

Available

High-Speed Video

Example zooms (Bright Field/Fluorescence)

LED High-Power Uniform Excitation

Channels

Gigapixel Image Stitching

RFP

Available

Bin240fps

Bin4120fps

Control software

For MCAM Computer

Viewing Software

For Windows and Linux

Open API

docs.ramonaoptics.com

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