UFB Application to Agriculture, Animal husbandry, Fishery, General Industry.
❶ Ministry of Economy, Trade and Industry Subsidies for management support for small andmedium enterprises (strategic fundamental technology advancement support project)

The development of unheated sterilization equipment for beverages such as milk by Ultra High Concentration Ultra Fine Bubble(UFB)． Specific R & D certification number：kinki1607030 [2016-2018]❷General Association of Mechanical Systems Promotion Association Grant Project[Strategy formulation concerning sterilization treatment of oyster by using fine bubble. [2017-2018]
Collaboration with the FBIA (Fine Bubble Industrial Association).

★ 1. TOSSLEC’s proprietary fine bubble generation technology ．
★ 2. Empirical validation for establishment of high efficiency production technology．
★ 3. Ultra fine bubble generator example．
★ 4 UFB grain size variation characteristics by control of bubble particle diameter concentration.
★ 5. Differences between our company UFB and other UFB.
★ 6. Selective adsorption / desorption washing sterilization principle.
★ 7. Oyster purification application of selective adsorption/desorption washing sterilization principle.
★ 8. Oyster norovirus substitute virus purification effect.
★ 9. Application to agriculture, animal husbandry, fishery industry, etc.
★ 10. Application of fine bubble generation technology Fish and shellfish animal feeder equipment.
★ 11. Verification and business process.
Aggregation and utilization of 'Knowledge'Member of the Industry-AcademiaGovernment Collaboration Association.
TOSSLEC Co.,Ltd. Fine Bubble Div.

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1.TOSSLEC’s proprietary fine bubble generation technology
Conventional technology＊Mixing FB and UFB

UFB generationmethod

Image diagram

・Pressure shearing melting method
・Swirling flow method
・Pressurized dissolution method
・Ultrasonic method
・Venturi method etc.

Our new technology＊ UFB is uniform （Homogeneousbubble）

Image diagram

FB：Bubble particle diameter less than 100μm.
UFB：Bubble particle diameter less than 1.0 μm.
MB：Bubble particle diameter of equal or greater than 1 μm to less than 100 μm.
※Definition of bubble particle size: According to ISO 20480-1

Proprietary new hybrid system
Japanese Patent No. 6123013
Name: Bubble-containing liquid manufacturing apparatus and Method
for producing bubble-containing liquid
Patent No. 6210917
Name: Nano bubble manufacturing equipment12 above-mentioned other patent applications filed，
PCT application 3 cases

Our proprietary new hybrid method generates micro uniform ultra high concentration bubbles (homogeneous bulls) by fine bubble continuous
crushing technology. At the time of bubble generation, the coagulation effect can be minimized, and energy utilization at FB collapse and UFB
ultra-high concentration result in non-heat sterilizationeffect.

❶ Under ultra pure water: 3 billion pieces / ml realization “NanoSight NS-300 measurement“❷ 30 billion cells / ml under low concentration alcohol "realized by NanoSight NS-300 measurement“❸ Bubble particle size / concentration control technology for target application❹ No choice of liquid / gas.
TOSSLEC Co.,Ltd. Fine Bubble Div.

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2.Example of UFB generator Outline drawing of washing sterilizer⇒Fine bubble is adapted to the object and cleaning and disinfection of foreign

matter of foreign matter and virus / bacteria with a small amount of water
current

The liquid bubble formation target is not limited in liquid type and gas type.
O2 , O3 , H2 , N2 , CO2 , NO , NH3 , Compressed air, CDA(Clean Dry Air) , Hypochlorous acid, Hydrogen chlorine
dioxide etc., Any type of pump can be used.
TOSSLEC Co.,Ltd. Fine Bubble Div.

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3.Characteristics of change in UFB particle by control of bubble particle
size concentration

Diameter：250nm Density：
1.5×10７ pieces/ml

Example Other Companies
Density is low
To vary

TOSSLEC Co.,Ltd. Fine Bubble Div.

Diameter：98nm Density：
2.8×10８ pieces/ml

Diameter：126nm Density：
3.14×10９ pieces/ml

UFB Diameter & Density
measurement
NanoSight NS-300

TOSSLEC‘s MethodDensity is controled，Can change greatly

４4. Differences between TOSSLEC’s UFB and other UFBs
(action on living fishes and shellfishes)
UFB trap phenomenon due to cap effect (act on the gills etc)
Passing rate of ozone UFB by precisionultrafiltration filter manufactured by NIPRO

Passing rate of ozone
AUFB(%)

10min

15min
Operationtime

TOSSTEC

20min

OTHERS(Reference)
Made by NIPRO Co., Ltd.
Precision ultrafiltration
MODEL CF-609N
Pore size 80 nm※1：Cap effectA phenomenon in which a large bubble blocks a passage in a narrow gap like a hat like ahat, and a small bubble that can pass through is trapped.

(TOSSLEC’s named)

When microbubbles adsorb to fish and shellfish, the cap effect works, it becomes impossible
to aspirate dissolved oxygen, the activity of fishes and shellfishes is drastically reduced, and insome cases it dies.
This phenomenon appears remarkably in aquaculture such as tuna, greater amberjack,yellowtail, etc. This phenomenon is also recognized in oysters etc. which aspirate a large
amount of seawater which is said to be 100 nm in gills distance.
TOSSLEC Co.,Ltd. Fine Bubble Div.

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5.Principle of selective adsorption / desorption washing sterilization(Bubble and virus · Bacteria (including spore and biofilm) , Application to adherent pesticides, particles, radionuclides, etc. ）Selective adsorption(Fig 1)

Desorption washing sterilization(Fig2)

Dielectric polarization
actionobjectobjectBacteria/virus
Bubble Retained charge

It is estimated that the surface of virus / bacteria has the property of bipolar electrolyte and negative charge UFB selectively adsorbs to virus and thelike and is detached and washed from the object to be cleaned.
In addition, the hydroxyl group (OH -) and the hydrogen group (H +) around the bubble are subjected to dielectric polarization, and there is a cleaning
action in a surface active state equivalent to that of soap. The anionic electrolyte is a factor to improve the bubble concentration.
Fig 1: Ultra fine bubble adheres, desorption and washing of the object by adsorption static elimination effect, at the same time bacteria loses
bioactivity by electric charge disappearance.
Fig2: t of collapse of the bubble Physically destroy cell nuclei by hydroxyl radicals, cavitation etc. Do not create resistant bacteria.

Application the time examples: CIP cleaning, washing sterilization of drinks such as milk, disinfection of bivalves such as oysters,
filter washing, wafer washing, intestinal flora control, etc.
TOSSLEC Co.,Ltd. Fine Bubble Div.

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6.Oyster purification technology by selective adsorption/ desorption washing sterilization principle

Pressure change at UFB conversion,
Ultraviolet light emission by ultrasonicexcitation, hydroxyl radical, etc.
Many energies are utilized for physicalsterilization.

New technology: Ultra fine bubble purification method

Selective adsorption / desorption washing sterilization of Norovirus and the like accumulated in the mid - gut line.

● Norovirus○Ultra FineBubble

Prior Art:UV sterilizing water, hypochlorous acid water (including
electrolytic hypochlorite),Ozone water can not be expected to
selectively adsorb and desorb washing bactericidal effect in vivo
such as oysters. "Bactericides reject aspiration by living organisms
such as oysters." UV does not act inside the oyster body.

Illustration of selective adsorption / desorption washing sterilization principleTOSSLEC Co.,Ltd. Fine Bubble Div.

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7.Oyster norovirus substitute virus purification result by model HMB-MAR015.
OysterRock oyster(produced in Hiroshima Prefecture)(produced in Kyoto)

UFB

UFB

beforebefore

UFB
after

UFB

UFB methodcleans up todetection limit

after

beforebeforeafter

UFB methodcleans up todetection limit

UVUV

UV

*Below detection limit

UV sterilization
methodUV

after

Infectivity(PFU/pies)

UV sterilization
methodInfectivity(PFU/pies)• Culture of feline calicivirus used in the experiment and extraction from oysters and evaluation of infectivity value are handled by Kitasato
Center for Environmental Science, Virus Department.
• The infectivity value is evaluated by the plaque method.
• Experimental place: conducted at the Kyoto Prefectural Ocean High School BSL2 (Biosafety Level 2) compliant tent.
• Used seawater: Kyoto prefectural marine high school sand filter equipment, ultraviolet sterilized one used.
• Virus handling and suction, freezing transportation method etc are all handled under the guidance of Kitasato Environment Science Center.
• Feline calicivirus: FDA (United States Food and Drug Administration) has certified it as a Noro virus replacement virus.

TOSSLEC Co.,Ltd. Fine Bubble Div.

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8.CO 2 / ozone UFB conversion resultby model HMB-OZ02

1. E. coli
NBRC NO.102203

２. S. aureus
NBRC NO.13276

３. B. cereus
NBRC NO.15305

NBRC number is a standard number managed by the Natural Resources Center of NITE (National Institute of Technology and Evaluation).
election of test bacteria is decided as representative indicator bacteria of beverages such as milk, such as beverage sterilization evaluation, in consultation with the Hokkaido Dairy
Test Inspection Association.
Test water used in the experiment: Ultrapure water treated with tap water with ion exchange resin was used
UFB Water Production: Sample prepared under each condition using our company's food washing sterilizer (model HMB - OZ 02) which used ozone and carbon dioxide for UFB waterproduction.
Measurement and handling of bacterial count: Associate Professor Food Processing Engineering Laboratory, Graduate School of Agriculture, Hokkaido University
Associate Professor Shigenobu Koseki. Development aiming at sterilization of spore bacillus (Bacillus cereus).

TOSSLEC Co.,Ltd. Fine Bubble Div.

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9.Application to agriculture, animal husbandry, fishery etc. (Expected effect)
Processed agricultural products
・ Promotion of growth and maintenance of health by bioactive effect.
・Long-term preservation by bactericidal effect (no preservative) by fruit juice bubble formation.
Poultry・Improvement of egg ratio.

Pig farming/Cow breeding etc
・Reduce mortality from illness / stress.

Vegetables / fruits tree etc.

・Growth acceleration, Improve immunity.

Marine products

・Growth acceleration
・Maintain freshness, wash bactericidal effect.
・Virus free.
・Biological non-heat sterilization．

TOSSLEC Co.,Ltd. Fine Bubble Div.

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10.Application example of fine bubble generation technology application equipment.

Model HMB－OZ02

Food cleaning and sterilizing
apparatus.

Model HMB－H201

Ultra high concentration hydrogenwater production.

Characteristic:
Ozone CIP, Drinking, hot bathingPurpose.

Model HMB－NHS01

Drinking suitable non-heated washing and sterilizing
apparatus for food poisoning bacteria (Escherichia coli,salmonella, spore-forming bacteria etc.) and various
gas UFB containing etc.

Characteristic:
Spore fungi sterilization, drinking
purpose non-heat sterilization．

Model HMB－MAR01

Fish and shellfish UFB generator.

Model HMB－MAR015

Fish and shellfish non-heated washing sterilizer.

Characteristic
Seedling and seed production utilizing physiologicalactivity effect, purification of bivalves such asoystersetc.

Application of sterilization technology and sterilization technology by crushing finebubble established by patent right.
Undertaking efforts focusing on non-heated washing sterilization technology suchas biological non-heated washing and sterilizing.

TOSSLEC Co.,Ltd. Fine Bubble Div.

Trademark ®
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