ISBC2012
The First International Symposium
on Biofunctional Chemistry

November 28-30, 2012
at Tokyo Tech Front,
Tokyo Institute of Technology, Japan

Organized by
Division of Biofunctional Chemistry,
The Chemical Society of JapanORGANIZING and SCIENTIFIC PROGRAM COMMITTEE
ORGANIZING COMMITTEE
Naoki SUGIMOTO, Chair
Tatsuya NABESHIMA, Vice-Chair
Shigetoshi AONO
Koichi FUKASE
Itaru HAMACHI
Kuniharu IJIRO
Yoshiki KATAYAMA
Hisakazu MIHARA
Shinichiro NISHIMURA
Takashi OHTSUKI
Yoshio OKAHATA
Keiko SHIMAMOTO
Mitsuhiko SHIONOYA
Jyunya SHIRAI
Hiroshi SUGIYAMA
Masahiro TAKAGI
Hitoshi TAMIAKIYasuteru URANO
Takehiko WADA
Yoshihito WATANABE

Konan University
University of Tsukuba
Okazaki Institute for Integrative Bioscience
Osaka University
Kyoto University
Hokkaido University
Kyushu University
Tokyo Institute of Technology
Hokkaido University
Okayama University
Tokyo Institute of Technology
Suntory Foundation for Life Sciences
The University of Tokyo
Takeda Pharmaceutical Company Limited
Kyoto University
Japan Advanced Institute of Science and Technology
Ritsumeikan University
The University of Tokyo
Tohoku University
Nagoya University

SCIENTIFIC PROGRAM COMMITTEE
Section I: Protein Interaction
Hisakazu MIHARA, Section Chair
Hitoshi TAMIAKITakashi OHTSUKITokyo Institute of Technology
Ritsumeikan University
Okayama University

Section II: Functional DNA/RNA
Naoki SUGIMOTO, Section Chair
Hiroshi SUGIYAMA
Takehiko WADA

Konan University
Kyoto University
Tohoku University

Section III: Metals in Chemical Biology
Tatsuya NABESHIMA, Section Chair
Mitsuhiko SHIONOYA
Shigetoshi AONO

University of Tsukuba
The University of Tokyo
Okazaki Institute for Integrative Bioscience

Section IV: Young Resarchers Society for Biochemistry
Osami SHOJI, Section Chair
Nagoya University
Yasutaka MATSUO
Hokkaido University
Eiji NAKATA
Kyoto University
Section V: Cell Function of Macromolecules
Koichi FUKASE, Section Chair
Masahiro TAKAGI
Yoshiki KATAYAMA

Osaka University
Japan Advanced Institute of Science and Technology
Kyushu University

Section VI: Nanobiotechnology Innovation in Biomolecular Chemistry
Itaru HAMACHI, Section Chair
Kyoto University
Kuniharu IJIRO
Hokkaido University
Yasuteru URANOThe University of Tokyo

ACCESS MAPCONFERENCE SCHEDULE
Registration and Information Desk
November 28th, 2012
8:30 -17:15 at Kuramae Hall
November 29th, 2012
8:30 -17:15 at Kuramae HallthNovember 30 , 2012
8:30 -17:15 at Kuramae Hall
Abstract, Name card, Request for receipts, Questions or assistance
Reception (invited speakers and organizers only)
Reception will be held on Wednesday, November 28th from 18:20 in Royal Blue Hall
Banquet (All participants)
Banquet will be held on Thursday, November 29th from 18:20 in Royal Blue Hall
Coffee and refreshments
Coffee and refreshments will be available in the Gallery

NOTE TO THE AUTHORS
Oral Presenters
One LCD projector with PC (Microsoft PowerPoint for Windows, Microsoft PowerPoint for Macintosh) will beavailable in the oral presentation room. It is expected that you will provide your own PC, and multiplug switches
for projector will be ready for you. Please remember
to bring your power cable to your presentation (AC 100V, 50 Hz). We also request all the speakers to bring theirdata by memory sticks (USB type) or CD-R’s, in case of trouble with the PC. Please bring the PPT file with theinformation of its version and your name and title.
---For Macintosh users
Please bring your external output terminal if it is not built into your own computer.
---Before presentation
During the break before your presentation, please hand a PC or a data to the projection staff. Mouse, laser pointerand clock will be prepared on the speakers desk. Please control their slides themselves.
---After presentation
Users of own PC expected to take off their PC after session at the speakers desk. Copy file will be completely
deleted by projection staff after the conference.
Poster Presenters
The size of a panel for a poster is 110 cm wide and 160 cm tall. Double-stick tape and thumbtacks will be suppliedat the site.
---Poster Set Up
8:30 - 12:00,
Wednesday, November 28th--- Discussion Time
13:40 - 14:20,
Wednesday, November 28th Odd-numbered
14:20 - 15:00,
Wednesday, November 28th Even-numbered
Odd-numbered
13:40 - 14:20,
Thursday, November 29thth
Even-numbered
14:20 - 15:00,
Thursday, November 29---Poster Removal
12:00 - 12:30, Friday, November 30th
Please keep the poster be presented during the symposium for the participants. Poster that is still on the board after
conference will be removed by the Secretariat.

DAYLY SCHEDULE

NOV.28 WED(ISBC 2012 PROGRAM at a Glance)

NOV.29 WEDNOV.30 WED

SectionⅠ

Section Ⅲ

Section Ⅴ

9:30-10:10

9:30-10:10

9:30-10:10

Donald Hilvert

Jung-Hye Roe

Carston R. Wagner(ETH Zurich, Switzerland)(Seoul National University, Korea)(University of Minnesota, USA)

10:10-10:50

10:10-10:50

10:10-10:50

Donald A. Bryant

Yoshitsugu Shiro

Ick-Chan Kwon

(Pennsylvania State University,(RIKEN SPring-8 Center, Japan)(Korea Institute of Science and9:20-9:30

Opening Remarks
Naoki Sugimoto(Chair of the Division, Konan
University, Japan)

USA)

Technology, Korea)

Coffee Break

10:50-11:10
11:10-11:50

11:10-11:50

11:10-11:50

Sihyun Ham

Yi Lu

Mikiko Sodeoka(Sookmyung Women's University,(University of Illinois at(RIKEN, Japan)

Korea)

Urbana-Champaign, USA)

Bert Poolman

11:50-12:30

Gerard Roelfes(University of Groningen, The

Takeaki Ozawa(University of Groningen, The

Netherlands)(The University of Tokyo, Japan)

Netherlands)

12:30-13:40

Lunch
Section Ⅵ
13:40-14:20

Frank Caruso
13:40-15:00

Poster session

13:40-15:00

Poster session(The University of Melbourne,
Australia)

13:40-14:20 odd numbers

13:40-14:20 odd numbers

14:20-15:00

14:20-15:00 even numbers

14:20-15:00 even numbers

Bing Xu(Brandeis University, USA)

Section Ⅱ

Section Ⅴ

15:00-15:40

15:00-15:30

Thomas Carell

Satoshi Yamaguchi(Ludwig-Maximilians-University,(The University of Tokyo, Japan)

Germany)

15:30-16:00

Jonathan B. Chaires(University of Louisville, USA)

16:00-16:30

Hirohide Saito(Kyoto University, Japan)

16:20-16:40

Coffee Break

16:30-17:00

Coffee Break

15:20-16:00

Vasilis Ntziachristos(Technical University of Munich,Akira Onoda(Osaka University, Japan)

15:40-16:20

15:00-15:20

Coffee BreakGermany)
16:00-16:40

Shoji Takeuchi(The University of Tokyo, Japan)

16:40-17:20【Plenary Lecture】

16:40-17:20

17:00-17:30

Yoshio Okahata

Kazuhiko Nakatani

Hiromu Kashida(Tokyo Institute of Technology,(Osaka University, Japan)(Nagoya University, Japan)

Japan)

17:20-18:00

17:30-18:00

Xiang Zhou

Satoshi Nishimura(Wuhan University, China)(The University of Tokyo, Japan)

17:20-17:30

Closing Remarks
Tatsuya Nabeshima(Vice Chair of the Division,
Tsukuba University)
18:20-

18:20-

Welcome Reception

Banquet(Royal Blue Hall)(Royal Blue Hall)

PROGRAMINVITED SPEAKERSNOVEMBER 28 WED (KURAMAE HALL)
9:20 – 9:30
Opening Remarks
Naoki Sugimoto
Chair of the Division, Konan University, JapanSECTION Ⅰ PROTEIN INTERACTION
9:30 – 10:10 (Chair: Hisakazu Mihara, Tokyo Institute of Technology)
O I-1

Designer Enzymes

Donald Hilvert
ETH Zurich, Switzerland___________________________________________________________________________________________

10:10 – 10:50 (Chair: Hitoshi Tamiaki, Ritsumeikan University)
O I-2

Characterization of Chlorosomes Containing Bacteriochlorophylls e and f
Donald A. Bryant
Pennsylvania State University, USA

Coffee Break (10:50 – 11:10)___________________________________________________________________________________________

11:10 – 11:50 (Chair: Hisakazu Mihara, Tokyo Institute of Technology)
O I-3

Understanding Protein Aggregation in Water

Sihyun Ham
Sookmyung Women's Univerisity, Korea___________________________________________________________________________________________

11:50 – 12:30 (Chair: Takashi Ohtsuki, Okayama University)
O I-4

Imaging and Control of Biological Functions Using Protein-Fragment Reconstitution
Analyses
Takeaki Ozawa
The University of Tokyo, Japan

Lunch (12:30 – 13:40)
Poster Session (13:40 – 15:00)

SECTION Ⅱ FUNCTIONAL DNA/RNA
15:00 – 15:40 (Chair: Takehiko Wada, Tohoku University)
O II-1The Chemistry of Stem Cell Development

Thomas Carell
Ludwig-Maximilians-University, Germany___________________________________________________________________________________________

15:40 – 16:20 (Chair: Naoki Sugimoto, Konan University)
O II-2 An Integrated Discovery Platform for G-Quadruplex Binding Drugs
Jonathan B. Chaires
University of Louisville, USA

Coffee Break (16:20 – 16:40)___________________________________________________________________________________________

16:40 – 17:20 (Chair: Naoki Sugimoto, Konan University)
O II-3

Small Organic Molecules Regulating RNA Structure and Function

Kazuhiko Nakatani
Osaka University, Japan___________________________________________________________________________________________

17:20 – 18:00 (Chair: Hiroshi Sugiyama, Kyoto University)
O II-4

Targeting to Nucleic Acid: Drug Design and Biomedical Applications
Xiang Zhou
Wuhan University, China

NOVEMBER 29 THU (KURAMAE HALL)SECTION Ⅲ METALS IN CHEMICAL BIOLOGY
9:30 – 10:10 (Chair: Shigetoshi Aono, Okazaki Institute for Integrative Bioscience)
O III-1 Metal-Dependent Gene Regulation in Bacteria
Jung-Hye Roe
Seoul National University, Korea___________________________________________________________________________________________

10:10 – 10:50 (Chair: Shigetoshi Aono, Okazaki Institute for Integrative Bioscience)
O III-2 Functional Characterization of Some Hemoproteins Based on Their Molecular Structures
Yoshitsugu Shiro
RIKEN SPring-8 Center, Japan

Coffee Break (10:50 – 11:10)___________________________________________________________________________________________

11:10 – 11:50 (Chair: Mitsuhiko Shionoya, The University of Tokyo)
O III-3 Metallo- DNAzymes and Their Applications in Directed Assembly of Nanomaterials, Sensing
and Imaging
Yi Lu
University of Illinois at Urbana-Champaign, USA___________________________________________________________________________________________

11:50 – 12:30 (Chair: Mitsuhiko Shionoya, The University of Tokyo)
O III-4

Enantioselective Catalysis by Artificial Metalloenzymes Based on DNA
Gerard Roelfes
University of Groningen, The Netherlands

Lunch (12:30 – 13:40)
Poster Session (13:40 – 15:00)

SECTION Ⅳ YOUNG RESEARCHERS SOCIETY FOR BIOCHEMISTRYg Researchers Society
for Biochemistry
15:00 – 15:40 (Chair: Eiji Nakata, Kyoto University)
O IV-1

Photo-Cleavable Chemical Tools Working at Biointerfaces for Externally Controlling
Biomolecules and Living Cells

Satoshi Yamaguchi
The University of Tokyo, Japan___________________________________________________________________________________________

15:30 – 16:00 (Chair: Yasutaka Matsuo, Hokkaido University)
O IV-2

Programmed Hemoprotein Assemblies toward Biodevices

Akira Onoda
Osaka University, Japan___________________________________________________________________________________________

16:00 – 16:30 (Chair: Yasuyuki Yamada, Nagoya University)
O IV-3Synthetic RNA-Protein Complexes for Controlling Cell Signaling
Hirohide Saito
Kyoto University, Japan

Coffee Break (16:30 – 17:00)___________________________________________________________________________________________

17:00 – 17:30 (Chair: Shinya Hagihara, Tohoku University)
O IV-4

Preparation of Novel Artificial Base Pairs for Functionalization of DNA

Hiromu Kashida
Nagoya University, Japan___________________________________________________________________________________________

17:30 – 18:00 (Chair: Tomoya Hirano, Tokyo Medical and Dental University)
O IV-5In Vivo Multi-Photon Molecular Imaging Technique Visualizes Parenchymal and Interstitial
Cell Cross-Talks in Chronic Inflammatory and Adult Common Disease Applications
Satoshi Nishimura
The University of Tokyo, JapanNOVEMBER 30 FRI (KURAMAE HALL)SECTION Ⅴ CELL FUNCTION OF MACROMOLECULES
9:30 – 10:10 (Chair: Koichi Fukase, Osaka University)
O V-1

Chemically Self-Assembled Nanostructures (CSANS) for Targeted Drug, Protein andOligonucleotide Delivery

Carston R. Wagner
University of Minnesota, USA___________________________________________________________________________________________

10:10 – 10:50 (Chair: Yoshiki Katayama, Kyushu University)
O V-2

Visualizing Enzyme Activities in Theragnosis
Ick-Chan Kwon
Korea Institute of Science and Technology, Korea

Coffee Break (10:50 – 11:10)___________________________________________________________________________________________

11:10 – 11:50 (Chair: Carston R. Wagner, University of Minnesota)
O V-3

Cell Death Control Molecules

Mikiko Sodeoka
RIKEN , Japan___________________________________________________________________________________________

11:50 – 12:30 (Chair: Kouhei Tsumoto, The University of Tokyo)
O V-4

Traffic in Crowded Environments and Osmosensing Mechanisms of Membrane Transport
Proteins
Bert Poolman
University of Groningen, The Netherlands

Lunch (12:30 – 13:40)

SECTION Ⅵ NANOBIOTECHNOLOGY INNOVATION IN BIOMOLECULAR CHEMISTRY
13:40 – 14:20 (Chair: Kuniharu Ijiro, Hokkaido University)
O VI-1Moving into the Biological Realm with Designer Particles Prepared via Materials Chemistry
and Template AssemblyFrank Caruso
The University of Melbourne, Australia___________________________________________________________________________________________

14:20 – 15:00 (Chair: Kuniharu Ijiro, Hokkaido University)
O VI-2Biological Functions and Applications of Self-Assembly of Small Molecules in Cellular
Environment
Bing Xu
Brandeis University, USA

Coffee Break (15:00 – 15:20)___________________________________________________________________________________________

15:20 – 16:00 (Chair: Itaru Hamachi, Kyoto University)
O VI-3

Multispectral Optoacoustic Tomography (MSOT) for Advancing Nano-Medicine

Vasilis Ntziachristos
Technical University of Munich, Germany___________________________________________________________________________________________

16:00 – 16:40 (Chair: Itaru Hamachi, Kyoto University)
O VI-4Microfluidic Technology for Biomolecular Processing

Shoji Takeuchi
The University of Tokyo, Japan___________________________________________________________________________________________

16:40 – 17:20 (Chair: Naoki Sugimoto, Konan University)
PL

A Translating Ribosome along mRNA Monitored on a Quartz Crystal Microbalance

Yoshio Okahata
Tokyo Institute of Technology, Japan___________________________________________________________________________________________

17:20 – 17:30
Closing Remarks
Tatsuya Nabeshima
Vice Chair of the Division, Tsukuba University, Japan

POSTER PROGRAMNOVEMBER 28 & 29

(POSTER SESSION SPACE)

40 minutes odd numbers, then 40 minutes even numbers

P1

Development of Zinc Finger Enzymes for Genome Engineering
Wataru Nomura, Akemi Masuda, Hirokazu Tamamura
Department of Medicinal Chemistry, Institute of Biomaterials and Bioengineering, Tokyo Medical and
Dental University

P2

Novel Methods for Blocking the Amyloidogenesis of Prion Proteins
Masatoshi Saiki1, Tomoya Miyazaki1, Hisayuki Morii2, Shinji Hashimoto1
1 Faculty of Engineering, Tokyo University of Science
2 National Institute of Advanced Industrial Science and Technology (AIST)P3

The Regulation Mechanism of Gene Expression in Translation Termination
Shuntaro Takahashi1,2, Naoki Sugimoto2,3, Yoshio Okahata2
1 Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University
2 Department of Biomolecular Engineering, Tokyo Institute of Technology
3 Faculty of Frontiers of Innovative Research in Science and Technology (FIRST),Konan University

P4

Selective Localization of Alzheimer’s Amyloid Beta and Peptide-Induced Membrane
Dynamics
Masamune Morita, Mun’delanji Vestergaard, Tsutomu Hamada, Masahiro Takagi
School of Materials Science, Japan Advanced Institute of Science and Technology

P5

Rational Alteration of Enzyme: Introduction of CH/π Interaction to the Transition State
Daiki Yoshida, Yasuko Nakano, Tadashi Ema
Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology,
Okayama University

P6

The Effect of Oxidized Cholesterol on the Membrane Dynamics Induced by Alzheimer’s
Amyloid Beta
Huong Thi Thanh Phan, Takahiro Hata, Masamune Morita, Tsuyoshi Yoda, Mun’delanji
Vestergaard, Tsutomu Hamada, and Masahiro Takagi
School of Materials Science, Japan Advanced Institute of Science and Technology

P7

Protein Labeling with Split SNAP-Tag by Protein–Protein Interaction Dependent
Reconstitution in Living Cells
Tatsuhiko Naoki, Masayasu Mie, Eiry Kobatake
Dept of Biological Information, Tokyo Institutive of Technology

P8

A Simple Approach for the Reconstitution of Hemoproteins
Norifumi Kawakami1, Osami Shoji2, Yoshihito Watanabe1
1 Research Center for Materials Science, Nagoya University
2 Department of Chemistry, Graduate School of Science, Nagoya University

P9

Holoabzyme and Site-Directed Chemical Mutation
Takeshi Tsumuraya, Fumihiro Ishikawa, Ikuo Fujii
Department of Biological Science, Graduate School of Science, Osaka Prefecture University

P10

Creation of Simultaneous Dual-Color Sensing System for Caspase Activities Using
Split-Fluorescent Proteins
Seiji Sakamoto, Anna Hugo, Mika Terauchi, Yasuyuki Araki, Takehiko Wada
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University

P11

Cell Selective Drug Delivery Using Cell Penetrating α-Helix Peptides Conjugated with Gold
Nanoparticles
Hyejin Park, Hiroshi Tsutsumi, Hisakazu Mihara
Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute ofTechnology

P12

Development of the Time-Resolved CD System for Detection of Structural Change Dynamics
of Biomolecules (XVII) - Study on the Dynamics of Photo-Induced Structural Change of
Photoactive Yellow Protein by Time-Resolved CD Measurement Makoto Murakami, Yoshiki Hamada, Yasuyuki Araki, Seiji Sakamoto, Mihoko Ui,
Kazushi Kinbara, Takehiko Wada
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University

P13

Development of New DNA Sensors Based on Unique Interactions between Hydrated Ionic
Liquids and DNAs
Hisae Tateishi-Karimaita1, Naoki Sugimoto1,2
1 Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University
2 Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University

P14

Suppression of Gene Expression Depending on Stability and Position of G-Quadruplexes inOpen Reading Frame
Tamaki Endoh1, Naoki Sugimoto1,2
1 Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University
2 Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University

P15

Construction of Stable DNA Three-way Junctions Based on Metal Complexation
Yusuke Takezawa, Jean-Louis H. A. Duprey, Shuhei Yoneda, Mitsuhiko Shionoya
Department of Chemistry, Graduate School of Science, The University of Tokyo

P16

Self-localizing Ligands: New Tools for Conditional Control of Protein Translocation and Cell
Signaling
Manabu Ishida1, Hideaki Watanabe2, Kazumasa Takigawa2, Yasutaka Kurishita3, Itaru
Hamachi3, Shinya Tsukiji1
1 Top Runner Incubation Center for Academia-Industry Fusion, Nagaoka University of Technology
2 Department of Bioengineering, Nagaoka University of Technology
3 Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering,
Kyoto University

P17

Physiological Function and Some Properties of Possible Aerotaxis Transducer Htr8 from
Extremely Halophilic Archaeon Haloarcula japonica

Toshitaka Matsubara1, Teppei Tadikara1, Yoshihiro Kubota1, Takayuki Kosaka1, Takatoshi
Ozawa1, Rie Yatsunami1, Toshiaki Fukui1, Kaoru Nakasone2, Nobuyuki Fujita3, Mitsuo Sekine3,
Tomonori Takashina4, Satoshi Nakamura1
1 Department of Bioengineering, Tokyo Institute of Technology
2 Department of Biotechnology and Chemistry, Kinki University
3 National Institute of Technology and Evaluation
4 Department of Applied Bioscience, Toyo University

P18

Analysis of Carotenoids in Haloarcula japonica and Characterization of Ha. japonica crtI
Homologs
Ai Ando1, Rie Yatsunami1, Shinichi Takaichi2, Toshiaki Fukui1, Kaoru Nakasone3, Nobuyuki
Fujita4, Mitsuo Sekine4, Tomonori Takashina5, Satoshi Nakamura1
1 Department of Bioengineering, Tokyo Institute of Technology
2 Nippon Medical School
3 Department of Biotechnology and Chemistry, Kinki University
4 National Insititute of Technology and Evaluation
5 Department of Applied Bioscience,Toyo University

P19

Use of Poly(ethylene glycol) for the Study of Nucleic Acid Interactions
Shu-ichi Nakano1,2, Hidenobu Hirayama1, Yuuichi Kitagawa1, Daisuke Yamaguchi1, Hayato
Sangen1, Daisuke Miyoshi1,2, Naoki Sugimoto1,2
1 Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University
2 Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University

P20

Molecular Crowding Effects on DNA G-Quadruplex
Daisuke Miyoshi1,2, Takeshi Fujimoto1, Shu-ichi Nakano1,2, Naoki Sugimoto1,2
1 Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University
2 Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University

P21

Single Molecule Level DNA Analysis Based on Charge Transfer Measurement
Kiyohiko Kawai1, Atsushi Maruyama2, Tetsuro Majima1
1 The Institute of Scientific and Industrial Research (SANKEN), Osaka University
2 Institute for Materials Chemistry and Engineering, Kyushu University

P22

The Invention of Intracellular Environmental Response Artificial Nucleic Acid (PRNA) and
its Applications to Cancer Cell Specific Oligonucleotide Therapeutics
Uematsu Ryo1, Mizutani Tatsuya1, Sakamoto Seiji1, ARAKI Yasuyuki1, Yamayoshi Asako2,
Nakase Ikuhiko3, Murakami Akira2, Futaki Shiroh3, Inoue Yoshihisa2, Wada Takehiko1
1 Institute of Multidisciplinary Research for Advanced Materials (IMRAM), Tohoku University
2 Kyoto Institute of Technology, 3Institute of Chemical Research, Kyoto University

P23

The Interaction between Sugar Linked Schiff Based Zn Complexes and DNA under the
Crowded Conditions
Misaki Nakai1, Akari Hirata1, Hironobu Fukuda1, Shigenobu Yano2,3, Yasuo Nakabayasi1
1 Materials and Bioengineering, Kansai University
2 Innovative Collaboration Center, Kyoto University
3 Graduate School of Material Science, Nara Institute of Science and Technolugy

P24

Development of Various Functional Fluorescent Molecules Based on the Construction of

Coumarin Library
Tomoya Hirano1, Takuya Shiraishi1, Haruka Sakai2, Marie Kinoshita2, Aya Tanatani2, Hiroyuki
Kagechika1
1 Institutes of Biomaterials and Bioengineering, Tokyo Medical and Dental University
2 Department of Chemistry, Faculty of Science, Ochanomizu University

P25

A Method for Site-Specific Glycosylation of Proteins
Azusa Yakata1, Kenichi Hatanaka2, Takashi Ohtsuki1
1 Graduate School of Science and Technology, Okayama University
2 Institute of Industrial Science, The University of Tokyo

P26

Synthesis of Proteins Including a Non-natural Amino Acid in Mammalian Cells
Takuma Ohtsuka1, Satoshi Neki1, Kodai Machida2, Hiroaki Imataka2, Takashi Ohtsuki1
1 Dept. of Med. Bioeng. Sci., Okayama University
2 Dept. of Mat. Sci. and Chem., University of Hyogo

P27

Development of Hypoxia-Sensitive Fluorescence Probes for Multicolor Imaging
Kenjiro Hanaoka, Wen Piao, Tetsuo Nagano
Graduate School of Pharmaceutical Sciences, The University of Tokyo

P28

Development of a Red Fluorescence Probe for Monitoring Dynamics of Cytoplasmic Calcium
Ion
Takahiro Egawa, Kenjiro Hanaoka, Yuichiro Koide, Tetsuo Nagano
Graduate School of Pharmaceutical Sciences, The University of Tokyo

P29

Role of Hydrogen Bonds in HutZ, Heme Degradation Enzyme from Vibrio cholerae
Yukari Sekine1, Takeshi Uchida1,2, Toshitaka Matsui3, Masao Ikeda-Saito3, Koichiro Ishimori1,2
1 Graduate School of Chemical Sciences and Engineering, Hokkaido University
2 Department of Chemistry, Faculty of Science, Hokkaido University
3 Institute of Multidisciplinary Research for Advanced Materials, Tohoku University

P30

Structural Basis for the Transcriptional Regulation of Heme Homeostasis in Lactococcus
lactis
Hitomi Sawai1, Hiroshi Sugimoto2, Masaru Yamanaka1, Yoshitsugu Shiro2, Shigetoshi Aono1
1 Okazaki Institute for Integrative Bioscience and Institute for Molecular Science, National Institutesof Natural Sciences
2 RIKEN SPring-8 Center, Harima Institute

P31

An Artificial Metalloenzyme; Utilization of Mononuclear Metalloprotein Containing 4-His
Metal Binding Motif
Nobutaka Fujieda, Ken-ichi Ishihama, Yuki Taniguchi, and Shinobu Itoh
Graduate School of Engineering, Osaka University

P32

Metal Cluster Formations and Their Functions in the Assembled Protein Cavity
Yuya Tanaka, Satoshi Abe, Takafumi Ueno
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology

P33

Preparation of Photoinduced Hydrogen Evolution System Using Membrane Bound
Hydrogenase with Phospholipid

Hidehiro Ito, Ichiro Okura, Toshiaki Kamachi
Department of Bioengineering, Tokyo Institute of Technology

P34

Heme Demetallation Enzyme from Vibrio cholerae
Takeshi Uchida1, Miho Sasaki2, Koichiro Ishimori1
1 Department of Chemistry, Faculty of Science, Hokkaido University
2 Department of Chemistry, School of Science, Hokkaido University

P35

Cytoprotective Effects of Nonheme Iron and Manganese Complexes with Superoxide
Dismutase Activity
Yutaka Hitomi1,2, Shoko Matsuda1, Akiko Nomura2, Yuji Iwamoto1, Akihiro Kashida1, Kengo
Arakawa1, Masashito Kodera1,2
1 Department of Molecular Chemistry and Biochemistry, Doshisha University
2 Centre for Nanoscience Research, Doshisha University

P36

Synthesis of Sugar Coated Lanthanide Nanoparticles for 5-Aminolevulinic Acid MediatedNear-Infrared Photodynamic Therapy
Atsushi Shimoyama1, Hiroya Watase1, Yu Liu1, Izumi Sotokawa1, Shun-ichiro Ogura1, Yuichiro
Hagiya1, Kiwamu Takahashi2, Katsushi Inoue2, Tohru Tanaka2, Hideya Yuasa1
1 Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
2 SBI Pharmaceuticals Co., Ltd.

P37

Unique Catalytic Activity of a Posttranslational Heterocyclase Revealed via an Artificial In
Vitro Biosynthesis System
Yuki Goto1,2, Yumi Ito1, Yasuharu Kato1, Shotaro Tsunoda1, Hiroaki Suga1
1 Department of Chemistry, Graduate School of Science, The University of Tokyo
2 PRESTO, JST

P38

Model of Avian Magnetic Compass: Artificial Radical Pair System Induced by Blue Light
Yoshimi Oka
Frontier Research Core for Life Sciences, University of Toyama

P39

Biosynthesis of Bacteriochlorophylls in Phototrophic Bacteria Performing Anoxygenic
Photosynthesis
Yusuke Tsukatani1, Haruki Yamamoto2, Jiro Harada3, Taichi Yoshitomi1, Tadashi Mizoguchi1,
Yuichi Fujita2, and Hitoshi Tamiaki1
1 Graduate School of Life Sciences, Ritsumeikan University
2 Graduate School of Bioagricultural Science, Nagoya University
3 Department of Medical Biochemistry, Kurume University School of Medicine

P40

Enhancement of the Hydrolytic Activity of Cellulose Nanocrystal Fibers by Novel Incubation
Methods
Hiromichi Okura1, Masahisa Wada2, Takeshi Serizawa1
1 Department of Organic and Polymeric Materials, Tokyo Institute of Technology
2 Graduate School of Agricultural and Life Science, The University of Tokyo

P41

Novel Strategy to Control Fluorescent Property of Asymmetric Xanthene Scaffold toward
Rationally Designable Fluorescent Probe
Eiji Nakata, Takashi Morii

Institute of advanced Energy, Kyoto University

P42

Penetrating Live Cells via a Screw Structured Motif of Bacteriophage T4
Nusrat J M Sanghamitra1, Hiroshi Inaba2, Dan Ohtan Wang1, Suji Kanamaru3, Fumio Arisaka3,
Kazuhiro Aiba1, Norio Nakatsuji1, Susumu Kitagawa1,2, Takafumi Ueno1,3
1 Institute of Integrated Cell-Material Science, Kyoto University
2 Graduate School of Engineering, Kyoto University
3 Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology

P43Interaction between Flavonoids and a Biomimetic Membrane
Bindu Chahal, Mun’delanji C.Vestergaard, Tsuyoshi Yoda, Masahiro Takagi
School of Materials Science, Japan Advanced Institute of Science and Technology

P44

Charge-Induced Transition in Membrane Mesoscopic Structures: Lateral Domains andVesicular Shapes
Hiroki Himeno, Tsutomu Hamada, Masahiro Takagi
School of Materials Science, Japan Advanced Institute of Science and Technology

P45

Development of the Detection System for Expressed Gene in Isolated Single Cells on
Compact Disk (CD)-Shaped Device
Shunsuke Furutani1, Naoki Shozen1, Hidenori Nagai2, Yuri Aoyama1, Izumi Kubo1
1 Department of Bioinformatics, Faculty of Engineering, SOKA university
2 National Institute of Advanced Industrial Science and Technology (AIST)P46

On the Performance of Newly Developed Near Infrared Window-Emitting Firefly Luciferin
Analogs for Deep Site Imaging
Satoshi Iwano, Obata Rika, Satoshi Kojima, Takashi Hirano, Shojiro Maki, Haruki Niwa
Department of Engineering Science, The University of Electro communications

P47

Porous Lysozyme Crystals as Reaction Vessels for Controlling Magnetic Properties of CoPt
Nanoparticles
Satoshi Abe, Takafumi Ueno
Department of Biomolecular Enginerring, Tokyo Institute of Technology

P48

Synthesis and Performance of a New Firefly Luciferin Analog Emitting Light within
Near-Infrared (NIR) Window
Kazuto Ito1, Nobuo Kitada1, Satoshi Iwano1, Rika Obata1, Shojiro Maki1, Haruki Niwa1, Tsuyoshi
Saito2, Shigeru Nishiyama2
1 Department of Engineering science, The University of Electro-Communications
2 Department of Chemistry, Faculty of Science and Technology, Keio University

P49

Development of New Firefly Luciferin Analogs
Chihiro Miura, Satoshi Iwano, Kazuto Ito, Takashi Hirano, Shojiro Maki, Haruki Niwa
Department of Engineering Sceience, The University of Electro-Communications

P50

Synthesis of Fluorescent Unnatural Streptavidin and Application to Molecular Biosensor for
BiotinXianwei Zhu1, Hiroaki Shinohara2
1 Graduate School of Innovative Life Science for Education, University of Toyama

2

P51

Graduate School of Science and Engineering for Research, University of Toyama

NMR Spectroscopic Approaches to the Conformational Dynamics of Oligosaccharides by
Paramagnetic Tagging
Takumi Yamaguchi1,2,3, Ying Zhang1,2,3, Koichi Kato1,2,3
1 Institute for Molecular Science and Okazaki Institute for Integrative Bioscience, National Institutesof Natural Sciences,
2 Sch. Phys. Sci., The Graduate University for Advanced Studies
3 Grad. Sch. Pharm. Sci., Nagoya City University

P52

Organic Nanotube Drug Nanocarrier
Wuxiao Ding, Mitsutoshi Masuda, Toshimi Shimizu
Nanotube Research Center, National Institute of Advanced Industrial Science and Technology (AIST)P53

Design and Construction of Hydrogels Composed of Gold Nanoparticle and Filamentous
Virus
Toshiki Sawada, Takeshi Serizawa
Department of Organic and Polymeric Materials, Tokyo Institute of Technology

P54

Shape-Dependent
Immunogenicity

Cellular

UptakesofGold

Nanoparticles

and

Their

VaccineTatsuya Matsunaga1, Kenichi Niikura2, Tadaki Suzuki3, Shintaro Kobayashi4, Hiroki
Yamaguchi4, Takafumi Ninomiya5, Hirofumi Sawa4, Kuniharu Ijiro2
1 Graduate School of Chemical Sciences and Engineering, Hokkaido University
2 RIES, Hokkaido University
3 Dept. of Pathol., NIID
4 CZC, Hokkaido University
5 Sapporo Medical University School of Medicine

P55

Molecular Design of Synthetic Viral Capsids
Kazunori Matsuura1, Yuusaku Mizuguchi2, Nobuo Kimizuka2
1 Graduate School of Engineering, Tottori University
2 Graduate School of Engineering, Kyushu University

P56

Evaluation of Importance of Nuclear Import in Artificial Viral Gene Delivery Using
Programmable Cellular Image Tracer
Takeshi Nagasaki1, Takeshi Kawazu1, Kazumi Hakamada2, Yusuke Oda3, Jun Miyake2,
Kazuo Maruyama3
1 Graduate School of Eeg., Osaka City University
2 Graduate School of Eng. Sci., Osaka University
3 Faculty of Pharm. Sci., Teikyo University

P57

Characterization of Crystallizing Lysozyme Solutions by Forward Light Scattering
Takashi Wakamatsu1, Hiroshi Koibuchi2
1 Department of Electrical and Electronics System Engineering, Ibaraki National College ofTechnology
2 Department of Mechanical and Systems Engineering, Ibaraki National College of Technology

P58

Application of Chaperone-like Activity of Amyloid Fibril of Peptides from αA-Crystallin forTreatment of Neurodegenerative Disorders
Sayuri Fukuhara, Tatsutoshi Nishigaki, Nobuhiko Tsuchiya, Tomonori Waku and Naoki Tanaka
Department of Bio-molecular Engineering, Kyoto Institute of Technology

P59

PET-Dependent Fluorescence Sensing of Enzymatic Reactions Using Large and Tunable
pKa Shifts of Aliphatic Amines
Yuji Oshikawa, Manabu Nakazono, Akio Ojida
Graduate School of Pharmaceutical Sciences, Kyushu University

P60

Design of a Highly Reactive Peptide Tag-Probe Pair for Selective Covalent Labeling of Cell
Proteins
Shigekazu Tabata1, Munetsugu Kido1, Kazushi Tani1, Itaru Hamachi2, Akio Ojida1
1 Graduate School of Pharmaceutical Sciences, Kyushu University
2 Department of Synthetic Chemistry and Biological Chemistry, Kyoto University

P61

Red/Near-Infrared Fluorescent Ar,O-BODIPY Dyes Bearing a Water-Soluble Tag: Their
Application to Cellar Staining
Masaki Yamamura, Shinya Yazaki, Michio Shimamura, Tatsuya Nabeshima
Graduate School of Pure and Applied Sciences & Tsukuba Research Center for Interdisciplinary
Materials Science,University of Tsukuba

P62

Unidirectional Cloning with a Sandwiched Zinc Finger NucleaseTomoaki Mori1,2, Kazuki Shinomiya1, Yasuhiro Aoyama1,3, Takashi Sera1,2
1 Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering,
Kyoto University
2 Graduate School of Natural Science and Technology, Okayama University
3 Faculty of Science and Engineering, Doshisha University.

P63

Encapsulation of Drugs in Virus-like Particles and Their Glutathione-Triggered Release in
Cells
Kenichi Niikura1, Naotoshi Sugimura 2, Hirofumi Sawa3, Kuniharu Ijiro1
1 Research Institute for Electronic Science, Hokkaido University
2 Graduate School of Chemical Sciences and Engineering, Hokkaido University
3 Research Center for Zoonosis Control, Hokkaido University