GenoMatrix™ Whole Genome
Amplification Kit(version A3)

Catalog No. 58001

Active Motif North America
1914 Palomar Oaks Way, Suite 150
Carlsbad, California 92008, USA
Toll free:877 222 9543
Telephone:
760 431 1263
Fax:
760 431 1351
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B-1330 Rixensart, Belgium
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0800 90 99 79
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Telephone: 		+32 (0)2 653 0001
Fax: 		
+32 (0)2 653 0050
Active Motif Japan
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Tokyo, 162-0824, Japan
Telephone:+81 3 5225 3638
Fax:
+81 3 5261 8733
Copyright 2011 Active Motif, Inc.

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Information in this manual is subject to change without notice and does not constitute a commitment on the part of Active Motif, Inc. It is supplied on an “as is” basis without any warranty of any
kind, either explicit or implied. Information may be changed or updated in this manual at any time.
This documentation may not be copied, transferred, reproduced, disclosed, or duplicated, inwhole or in part, without the prior written consent of Active Motif, Inc. This documentation is
proprietary information and protected by the copyright laws of the United States and international treaties.
The manufacturer of this documentation is Active Motif, Inc.
© 2011 Active Motif, Inc., 1914 Palomar Oaks Way, Suite 150; Carlsbad, CA 92008. All rights reserved.
All trademarks, trade names, service marks or logos referenced herein belong to their respective
companies.
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TABLE OF CONTENTS

Page

Overview  . . . . . . .  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
GenoMatrix Whole Genome Amplification Kit Advantages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Kit Components and Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Additional Materials Required . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
GenoMatrix Whole Genome Amplification Kit Experimental Design  . . . . . . . . . . . . . . . . . . . . 3
Protocols – GenoMatrix Whole Genome Amplification Kit
A. DNA Shearing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
B. Preparation of PCR-amplifiable DNA Fragment Library . . . . . . . . . . . . . . . . . . . . . . . . . . 4
C. Library Amplification  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
References . . . . . .  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
Appendix
Section A. Whole Genome Amplification from ChIP Samples . . . . . . . . . . . . . . . . . . . . . . . 7
Section B. Whole Genome Amplification from MethylCollector™ Samples . . . . . . . . . . . 8
Section C. Representative, Comparative Data from Multiple Loci . . . . . . . . . . . . . . . . . .  10
Section D. Related Products  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  10
Technical Services  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

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Overview
Whole Genome Amplification (WGA) has emerged as a fundamental method for DNA analysis
from limited quantities of genomic DNA in forensic analysis and disease gene discovery. The WGA
technique allows for amplification of the whole genome, preserving what was a limited sample
while enabling analysis of any gene in the organism being studied. Active Motif’s GenoMatrix™ Whole Genome Amplification Kit makes it possible for users to achieve up to a 500-foldamplification of genomic DNA starting from nanogram quantities of DNA while maintaining thesequence representation of the starting material. Several strategies for WGA have been developed
during the past decade, each with variable fidelity, yield and coverage of the amplified genome(see References for examples of these different methods). The GenoMatrix Kit provides all thereagents and buffers to quickly and easily perform a genome-representative amplification of any
type of genomic DNA. In particular, the method works especially well with ChIP samples as well
as samples generated using Active Motif’s MethylCollector™ and UnMethylCollector™ Kits. All
reagents included in the GenoMatrix Whole Genome Amplification Kit have been optimized forhigh-efficiency genome amplification.

product

formatcatalog no.

GenoMatrix™ Whole Genome Amplification Kit

50 rxns

58001

The GenoMatrix™ Whole Genome Amplification Kit is for research use only. Not for use indiagnostic procedures.

GenoMatrix Whole Genome Amplification Kit Advantages
Preserves the Sequence Representation of the Starting Genomic DNA
Active Motif’s GenoMatrix Whole Genome Amplification Kit utilizes a new approach that virtuallyeliminates amplification bias, so the amplified material has the same sequence representation as
the material that you started with.
Quick and Simple
The entire procedure can be completed in less than two and half hours.
Versatile
GenoMatrix can be used as a stand alone product to amplify unsheared DNA or with samples
generated by Active Motif’s ChIP-IT™ Express, MethylCollector™ and UnMethylCollector™ Kits.
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Kit Components and Storage
Please store each component at the temperature indicated in the table below.
Reagents

Quantity

Storage / Stability

10X Shearing Buffer

55 µl-20°C for 6 months

GenoMatrix Buffer 1

200 μl

-20°C for 6 months

GenoMatrix Buffer 2

250 µl

-20°C for 6 months

GenoMatrix DNA polymerase

55 µl-20°C for 6 months

GenoMatrix Primer A

60 µl-20°C for 6 months

GenoMatrix Primer B

150 μl

-20°C for 6 months

10X PCR Buffer

1.5 ml

-20°C for 6 months

Taq DNA polymerase

45 µl-20°C for 6 months

dNTPs (5 mM each)

450 µl

-20°C for 6 months

Human Genomic Control DNA

12 µl-20°C for 6 months

Additional materials required• Sterile water• Pipettors and filter tips
• PCR tubes• Thermal cycler• DNA purification columns (e.g. Active Motif’s Chromatin IP DNA Purification Kit, Catalog
No. 58002). Any columns used should have the ability to retain ssDNA as well as dsDNA.
• Spectrophotometer or equivalent to quantify DNA. The method used must be able toquantify ssDNA as well dsDNA. (Measurement techniques such as PicoGreen that do notdetect ssDNA will often understate the total amount of DNA present, as single-stranded
DNA may be generated during amplification.)

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GenoMatrix Whole Genome Amplification Kit
Experimental Design
PLEASE READ THE ENTIRE PROTOCOL BEFORE STARTING!
Active Motif’s GenoMatrix Whole Genome Amplification Kit includes sufficient reagents toperform 50 whole genome amplification reactions. Each sample must contain a minimum of
10 ng of DNA for the process to work efficiently. The kit enables you to generate a representative
amplification of any kind of genomic DNA.
The GenoMatrix Whole Genome Amplification process is divided into DNA fragmentation, library
preparation and library amplification. The first two steps, fragmentation and library preparation,
should be carried out without interruption, as the ends of the library DNA can degrade.
The library DNA can be stored up to one week at -20°C without detectable differences in the
results. The final GenoMatrix Whole Genome Amplification DNA should be stored at -20°C and is
as stable as any comparable genomic DNA.
Step 1 (Protocol A)
The starting DNA samples are subjected to a random shearing.
Note:

This step is omitted if the DNA samples have already been sheared by anothermethod, such as subsequent to chromatin immunoprecipitation (ChIP) or as theresult of MethylCollector or UnMethylCollector experiments.

Step 2 (Protocol B)
In the second step, the sheared DNA samples are converted from small DNA fragments of
unknown sequence into PCR amplifiable molecules flanked by known sequences.
Step 3 (Protocol C)
The resultant DNA from Step 2 are then PCR amplified using specific oligonucleotide primers. The
kit requires a minimum of 10 ng of starting DNA, which can yield up to 5 µg of Whole Genome
Amplified product (up to a 500-fold amplification of the original genomic DNA). The GenoMatrix
Whole Genome Amplification Kit contains an optimized DNA polymerase that decreases the
background in the reaction. After purification, the amplified product can be analyzed in a manner
similar to any DNA sample. A number of downstream applications may be performed including
microarray hybridizations, quantitative PCR, etc.
Notes Before Starting:• Always run a control sample with water only (no DNA template) to ensure that the reagentsare not contaminated with DNA.
• Run a positive control sample such as the human genomic control DNA included in the kit
along with your samples.
• Use filter tips and gloves during the entire procedure to prevent DNA contamination.

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Protocols
The GenoMatrix Whole Genome Amplification Kit requires a minimum of 10 ng starting DNA ina volume of no more than 11 µl. Insufficient (or too dilute) starting material can result in poor
genomic representation in the final product.
It is recommended that you perform the protocol in 0.2 ml PCR tubes so that you can use athermal cycler for the incubation steps.
If you are working with unsheared genomic DNA, begin with Protocol A. If your samples are theresult of chromatin immunoprecipitation (ChIP), MethylCollector, UnMethylCollector or someother procedure that results in sheared DNA, begin with Protocol B.
See the Appendix for information regarding the use of GenoMatrix with ChIP samples (Appendix –Section A) or with MethylCollector and UnMethylCollector samples (Appendix – Section B).

A. DNA Shearing
1.

Quantify your DNA sample, then prepare a 1 ng/µl DNA solution.

2.

Add 10 µl of the DNA solution (1 ng/µl) to a clean PCR tube.

3.

Add 1 µl of 10X Shearing Buffer the 10 µl of DNA solution in the PCR tube.

4.

Place the tube in a thermal cycler and heat at 95°C for exactly 4 minutes.

5.

Immediately cool the sample on ice, then centrifuge briefly to collect the liquid in thebottom of the tube. Return the sample to ice or 4°C immediately after centrifugation.

B. Preparation of PCR-amplifiable DNA Fragment Library1.

Add 1 µl of GenoMatrix Primer A (40 µM) to each sample.

2.

Add 3 µl of GenoMatrix Buffer 1 and mix well by vortexing. If necessary, centrifuge briefly tocollect the liquid in the bottom of the tube.

3.

Heat for 2 minutes at 95°C (in a thermal cycler). Cool immediately on ice, centrifuge briefly
and return to ice for 5 minutes.

4.

Add 4 µl of GenoMatrix Buffer 2 to samples.

5.

Add 1 µl of GenoMatrix DNA polymerase.

6.

Vortex thoroughly and centrifuge briefly.

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7.

Place samples in a thermal cycler and incubate as follows:a.

16°C for 20 minutesb.

24°C for 20 minutesc.

37°C for 20 minutes

d.

75°C for 5 minutes

Hold at 4°C
8.

Remove samples from thermal cycler and centrifuge briefly. Samples may be amplified
immediately or stored at -20°C for up to one week before Library Amplification.

Section C. Library Amplification
1.

Dilute samples with distilled water to a final volume of 60 µl (add 40 µl of sterile dH2O).

2.

Prepare a master mix with the following components:
Reagent

Per Reaction

10X PCR Buffer

10 µl

dNTPs

8 µl

GenoMatrix Primer B

2 µl

Taq DNA polymerase

0.8 µldH2O64.2 µl

Total

85 µl

In fresh PCR tubes, add 85 µl of this mix to 15 µl of your diluted sample.
3.

Put samples in a thermal cycler and perform 20-30 cycles as follows:a.

94°C for 30 secondsb.

40°C for 30 seconds

c.

50°C for 30 seconds

d.

72°C for 2 minutes

Hold at 4°C
4.

Remove samples from thermal cycler and centrifuge briefly to collect the liquid in thebottom of the tube. Maintain the reactions at 4°C or store at -20°C until ready for analysis or
purification. The GenoMatrix Whole Genome Amplification DNA is now as stable as any typeof genomic DNA stored under the same conditions.

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5The quality and the average size of the GenoMatrix Whole Genome Amplification DNA can also
be determined by loading 5-10 µl of the final product on a 1.5-2% agarose gel. A “smear” of DNA
should be present between 300 bp and 1 kb.

Figure 1. Agarose gel displaying the results of the GenoMatrix Whole Genome Amplification Kit.
Lane 1: DNA molecular weight marker.
Lane 2: GenoMatrix Kit using 10 ng of Human Genomic Control DNA as the starting material.
Lane 3: GenoMatrix Kit negative control with no starting DNA.

It is recommended to purify the final product before being it is used in subsequent applications(DNA microarray hybridization, etc.). We recommend using Active Motif’s Chromatin IP DNA
Purification Kit (Catalog No. 58002) or equivalent columns that retain single-stranded DNA as wellas double-stranded DNA. Once purified, the DNA can be quantified by measuring absorbanceassuming that 1 A260 unit is equivalent to 50 ng/µl DNA.

References
1.
2.
3.
4.
5.
6.

Sun G. et al., 2005 Legal Med 7: 279-286.
Barker D.L. et al., 2004 Genome Research, 14: 901-907.
Hughes S. et al., 2005 Prog Biophys Molec Biol 88: 173-189.
Lasken R.S. and Egholm M., 2003 Trends Biotech 21: 531-535.
Hawkins T.L., Detter J.C., Richardson P.M., 2002 Curr Opin Biotech 13: 65-67.
Dean F.B. et al., 2002 PNAS 99: 5261-5266.

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Appendix
Section A. Whole Genome Amplification from ChIP Samples
At the end of chromatin immunoprecipitation (ChIP), the amounts of DNA collected are oftenvery low. Certain techniques (e.g. microarray hybridization) require a larger amount of DNA thanresults from the average ChIP experiment. You should determine the minimal amount ofchromatin you must start with in order to collect at least 10 ng of DNA.
To help you, the following chart estimates the ChIP output (DNA collected) for different quantities of starting chromatin.
Number of cells

Chromatin input

ChIP DNA output

4

200 ng30 pg^

52 µg

300 pg^5 x 105

10 µg

1.3 ng*1 x 106

20 µg

10 ng

1 x 107200 µg

150 ng*1 x 101 x 10* output measured from a RNA pol II ChIP sample^ output estimated based on ChIP samples from larger scale experiments
Table 1. Estimates of DNA resulting from ChIP based on the number of mammalian cells used for each ChIP.

Depending on the efficiency of the antibody being used for ChIP and on the protein that is being
immunoprecipitated, the amount of DNA collected will vary. With histone antibodies, the collected amounts of DNAs are often larger. Inversely, with antibodies targeted to rare proteins, less
DNA will be collected, so you should perform your ChIP beginning with more chromatin.
Before starting the GenoMatrix Whole Genome Amplification procedure, a purification of yourChIP samples is required. This will remove anything in the ChIP buffers that might prevent amplification of the DNA. It also allows you to concentrate your samples, which is important because
for the GenoMatrix procedure to work effectively, the volume of your DNA samples should notexceed 10 µl. We recommend using the Active Motif’s Chromatin IP DNA Purification Kit (Catalog
No. 58002) or equivalent. Samples should be eluted in 10 µl of distilled water. An alternative isphenol-chloroform extraction / ethanol precipitation. For maximum efficiency, include glycogen in
the precipitation. After washing and drying the pellet, resuspend it with 10 µl distilled water. One
microliter of purified DNA is then used for quantification.
Once your ChIP DNA samples have been purified and quantified, the GenoMatrix procedure canbe started. But, as ChIP DNA has already been sheared, you do not begin with the fragmentation
step. Begin with Protocol B on page 4. Make sure that your ChIP DNA is 1 ng/µl, and use 10 µl of it
to prepare the PCR-amplifiable DNA Fragment Library.
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40
35
30

RNA pol II
Input DNA
Neg IgG

25
20
15
10
5
0
p53

GAPDH

Figure 2. Quantitative PCR carried out on ChIP DNA samples
Following chromatin immunoprecipitation with RNA polymerase II antibody (purple bars) and Negative control IgG (redbars), the ChIP DNA samples were amplified using the GenoMatrix Whole Genome Amplification Kit. qPCR was thenperformed on the amplified samples and on the Input DNA used in the ChIP with primers specific for either the p53 or
GAPDH promoters.

Section B. Whole Genome Amplification Starting with Samples
Resulting from Experiments using the MethylCollector Family of
DNA Methylation Detection Kits
The GenoMatrix Whole Genome Amplification procedure requires a minimum of 10 ng of purified
CpG methylated or unmethylated DNA as starting material. However, if MethylCollector Ultra(Catalog No. 55005) or UnMethylCollector (Catalog No. 55004) Kits will be used to enrich formethylated or unmethylated CpG islands before beginning the GenoMatrix procedure, we recommend using 500 ng of fragmented DNA as the starting material in either the MethylCollector
Ultra or UnMethylCollector Kits. The amount of DNA recovered from the MethylCollector Ultra
and UnMethylCollector assays will depend on the global methylation status of the sample DNA.
By starting with 500 ng of fragmented DNA, it should be possible to recover 10 ng or more after
enrichment and purification of the sample.

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8Following enrichment, the samples must purified before starting the GenoMatrix procedure.
Samples can be purified either by performing a phenol/chloroform extraction followed by ethanol
precipitation, or by using Active Motif’s Chromatin IP DNA Purification Kit (Catalog No. 58002).
Purified DNA should be eluted in 10 µl DNase-free water. Use 1 µl of your sample for quantification.
A minimum of 10 ng DNA is required before starting the GenoMatrix Whole Genome Amplification procedure.
After quantification of the purified CpG methylated or unmethylated DNA, the DNA should bediluted to a final concentration of 1 ng/µl in DNase-free water and 10 µl (10 ng) will be used in the
GenoMatrix Whole Genome Amplification Kit. Because the starting material for MethylCollector
Ultra and UnMethylCollector was fragmented (either by enzymatic digestion or sonication), thefragmentation step can be skipped. Begin the GenoMatrix procedure with Protocol B on page 4.

Figure 3. Real time quantitative PCR used to analyze samples isolated using MethylCollector Ultra.
500 ng of human male genomic DNA was processed using the MethylCollector Ultra Kit. Eluted and Unbound DNA samples
were purified and 10 ng of each fraction was subjected to the GenoMatrix Whole Genome Amplification procedure. Then,
10 ng of purified GenoMatrix DNA samples were analyzed by real time PCR using primers specific for the promoter of amethylated gene, SNRPN, which should be bound by the MethylCollector assay, and end up in the Eluted fraction.

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9Section C. Representative, Comparative Data from Multiple Loci
For any Whole Genome Amplification procedure to be effective, it must to amplify the genomic
DNA while preserving the DNA sequence representation of the starting material. The GenoMatrix
Whole Genome Amplification Kit has been validated using primers spanning the genome, as wellas used in comparison to a competitor’s whole genome amplification kit.
35
30

Ct Value

25
20
15
10
5
0

BRCA1

BRCA2

CREB1

DHFR

FosB

MAS1

NEDD4

NFκB

Figure 4. Quantitative PCR carried out on DNA samples using primers from a sampling of representative genes.
Quantitative real-time PCR was carried out on DNA samples using primers indicated below each data set and the Ct value
for each sample plotted. The DNA was derived from an original DNA sample amplified using the GenoMatrix Whole
Genome Amplification Kit (red bars), amplified with a competitor’s whole genome amplification kit (copper bars) or not
subjected to whole genome amplification (purple bars).

Section D. Related Products
ChIP-IT™ Kits		

FormatCatalog No.

ChIP-IT™ Express		ChIP-IT™ Express Enzymatic		
ChIP-IT™ Express Shearing Kit		
ChIP-IT™ Express Enzymatic Shearing Kit		
ChIP-IT™ Express HT		
Re-ChIP-IT™		
Chromatin IP DNA Purification Kit		
EpiShear™ Sonicator		ChIP-IT™ Protein G Magnetic Beads		
Siliconized Tubes, 1.7 ml		
ChIP-IT™ Control Kit – Human		
ChIP-IT™ Control Kit – Mouse		ChIP-IT™ Control Kit – Rat		
Ready-to-ChIP HeLa Chromatin		
Ready-to-ChIP Hep G2 Chromatin		
Ready-to-ChIP K-562 Chromatin		
Ready-to-ChIP NIH/3T3 Chromatin		Bridging Antibody for Mouse IgG		

25 rxns
25 rxns
10 rxns
10 rxns
96 rxns
25 rxns
50 rxns
110 V
25 rxns
25 tubes
5 rxns
5 rxns
5 rxns
10 rxns
10 rxns
10 rxns
10 rxns
500 µg

53008
53009
53032
53035
53018
53016
58002
53051
53014
53036
53010
53011
53012
53015
53019
53020
53021
53017

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DNA Methylation		

FormatCatalog No.

hMeDIP		
MethylDetector™		
MethylCollector™		
MethylCollector™ Ultra		
UnMethylCollector™		
DNMT Activity / Inhibition Assay		
Methylated DNA Standard Kit		
Fully Methylated Jurkat DNA		
Jurkat genomic DNA		

10 rxns
50 rxns
25 rxns
30 rxns
30 rxns
96 rxns3 x 2.5 µg
10 µg
10 µg

55010
55001
55002
55005
55004
55006
55008
55003
55007

ChIP-validated Histone Antibodies

FormatCatalog No.

Application

Histone H2A pAb
ChIP, WB
200 µl
39235
Histone H2A phospho Ser129 pAb
ChIP, IF, IP, WB
200 µl
39271
Histone H2A.Z pAb
ChIP, WB
200 µl39113
Histone H2B pAb
ChIP, WB
200 µl
39237
Histone H2B acetyl Lys5 pAb
ChIP, WB
200 µl
39123
Histone H2B acetyl Lys12 pAb
ChIP, WB
100 µl39669
Histone H2B acetyl Lys16 pAb
ChIP, WB
200 µl39121
Histone H2B acetyl Lys46 pAb
ChIP, WB
200 µl
39571
Histone H2B acetyl Lys120 pAb
ChIP, WB
200 µl39119
Histone H3, C-terminal pAb
ChIP, WB
200 µl
39163
Histone H3 acetyl pAb
ChIP, WB
200 µl39139
Histone H3 acetyl Lys4 pAb
ChIP, IF, WB
200 µl
39381
Histone H3 monomethyl Lys4 mAb
ChIP, WB
100 µg39635
Histone H3 dimethyl Lys4 pAb
ChIP, WB
200 µl
39141
Histone H3 trimethyl Lys4 pAb
ChIP, WB
200 µl
39159
Histone H3 dimethyl Lys9 pAb
ChIP, IF, WB
200 µl
39239
Histone H3 trimethyl Lys9 pAb
ChIP, WB
200 µl39161
Histone H3 acetyl Lys9 pAb
ChIP, WB
200 µl39137
Histone H3 acetyl Lys14 pAb
ChIP, WB
200 µl39599
Histone H3 acetyl Lys18 pAb
ChIP, IF, WB
200 µl
39587
Histone H3 acetyl Lys23 pAb
ChIP, IF, WB
200 µl39133
Histone H3 acetyl Lys27 pAb
ChIP, IF, WB
200 µg39133
Histone H3 acetyl Lys27 pAb
ChIP, WB
200 µl39135
Histone H3 dimethyl Lys27 pAb
ChIP, IF, WB
200 µl
39245
Histone H3 trimethyl Lys27 mAb
ChIP, WB
200 µl
39535
Histone H3 trimethyl Lys27 pAb
ChIP, IF, WB
39155
200 µgHistone H3 trimethyl Lys27 pAb
ChIP, WB
200 µl
39156
Histone H3 acetyl Lys36 pAb
ChIP, IF, WB
200 µl
39379
Histone H3 acetyl Lys56 pAb
ChIP, WB
200 µl
39281
Histone H3 acetyl Lys64 pAb
ChIP, IF, WB
200 µl
39545
Histone H3 acetyl Lys79 pAb
ChIP, WB
200 µl
39565
Histone H4 pan-acetyl pAb
ChIP, IF, WB
200 µl
39243
Histone H4 tetra-acetyl pAb
ChIP, WB
50 µl
39179
Histone H4 acetyl Lys5 pAb
ChIP, IF, WB
200 µl
39169
Histone H4 acetyl Lys5 pAb
ChIP, IF, WB
200 µl
39583
Histone H4 acetyl Lys12 pAb
ChIP, IF, WB
200 µl
39165
Histone H4 acetyl Lys16 pAb
ChIP, WB
200 µl
39167
Histone H4 monomethyl Lys20 pAb
ChIP, IF, WB
200 µl
39175
Histone H4 trimethyl Lys20 pAb
ChIP, IF, WB
200 µl39180
For an up-to-date list of over 125 ChIP-validated antibodies, please visit www.activemotif.com/chipabs.
Application Key: ChIP = Chromatin Immunoprecipitation; EMSA = Electrophoretic Mobility Shift Assay;
IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; WB = Western blot
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11Technical Services
If you need assistance at any time, please call Active Motif Technical Service at one of thenumbers listed below.
Active Motif North America
1914 Palomar Oaks Way, Suite 150
Carlsbad, CA 92008
USA
Toll Free:877 222 9543
Telephone: 760 431 1263
Fax:
760 431 1351
E-mail:tech_service@activemotif.com
Active Motif Europe
104 Avenue Franklin Roosevelt
B-1330 Rixensart, Belgium
UK Free Phone:		
0800 169 31 47
France Free Phone:
0800 90 99 79
Germany Free Phone: 0800 181 99 10
Telephone:		+32 (0)2 653 0001
Fax:		
+32 (0)2 653 0050
E-mail:		
eurotech@activemotif.com
Active Motif Japan
Azuma Bldg, 7th Floor
2-21 Ageba-Cho, Shinjuku-Ku
Tokyo, 162-0824, Japan
Telephone: +81 3 5225 3638
Fax:
+81 3 5261 8733
E-mail:japantech@activemotif.com
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