AbFlex® KRAS antibody (rAb)
Catalog Nos: 91423, 91424
RRID: AB_3216311
Application(s): ELISA, WB
Reactivity: Human

Quantities: 100 µg, 10 µg
Purification: Protein A Chromatography
Host: Rabbit
Isotype: IgG
Molecular Weight: 24 kDa

Background: AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences
to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for
enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels
directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.
AbFlex® KRAS antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype)in mammalian 293 cells.
Oncogenic mutations in Ras genes have been found in about 30% of human cancers. This family of genes is involved in cellsignaling pathways that control cell growth and death. The human Ras gene family consists of three identified members H-, Kand N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons. Human H- and K-ras are the homologues of vH- and v-K-ras, sequences. Under normal circumstances the Ras-GTPase activity gradually converts GTP to GDP, but inoncogenic mutations Ras locks the protein into the active GTP conformation. This leads to a constant Ras gene anduncontrollable cell growth. Due to these intracellular relationship Ras antibodies have emerged as a valuable tool for target
validation studies for cancer.
Immunogen: This information is proprietary to Active Motif and/or its suppliers.

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodiumazideis highly toxic.
Application Notes:
Applications Validated by Active Motif:
0.5 - 2 µg/ml
ELISA: Individual optimization may be required.
Storage and Guarantee: Some products may be shipped at room temperature. This will not affect their stability orperformance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2years. Keep all reagents on ice when not in storage. This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.

Application Key: ChIP = Chromatin Immunoprecipitation; FACS = Flow Cytometry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; WB = Western Blot

North America 877 222 9543 • Europe +32 (0)2 653 0001 • Japan +81 (0)3 5225 3638 • www.activemotif.com

AbFlex® KRAS Antibody (rAb) tested by Western blot.
NTERA whole cell extract (20 µg) was probed with AbFlex® KRAS antibody
at 0.5 mg/ml.

AbFlex® KRAS Antibody tested by bead-based assay multiplex ELISALuminex bead-based specificity analysis was used to confirm the specificityof KRAS antibody for recombinant human RAS protein isoforms conjugatedto MagPlex Luminex beads. Peptide-bound antibody was detected with antimouse IgG-Phycoerythrin and read in a Luminex instrument. (Luminex® is a
registered trademark of Luminex Corporation.)

North America 877 222 9543 • Europe +32 (0)2 653 0001 • Japan +81 (0)3 5225 3638 • www.activemotif.com