Sono-photodynamic Therapy
on Cancer and Bacteria
Literature Seminar
2023/9/14
Keiichi Kawabata (M2)

Contents• Introduction• Sono-photodynamic Therapy on Cancer• Sono-photodynamic Therapy against Bacteria• Summary2

Contents• Introduction• Sono-photodynamic Therapy on Cancer• Sono-photodynamic Therapy against Bacteria• Summary3

Photodynamic Therapy
PDT✅High selectivity✅ Low risk of side effects❌Low permeability
Light
S1

radicalISC
T1

S0
sensitizer

1O
2
3O
2

sensitizer
4

Sonodynamic TherapySDT✅ Non-invasive✅ High permeability

Bogdan, et al., Nanoscale Res. Lett., 2017, 12, 225
5

Cavitation

J, Ö., et al., Sustainable and energy efficient leaching of 6tungsten (W) by ultrasound controlled cavitation, 2017, p8

Mechanism of SDTXing, X. et al., Coordination ChemistryReviews, 2021, 445, 214087
7Sensitizers
OHNOH

N

N

HNNH

HNNH
NI
O

NF

CO2NaCl

HN

OH

MeO

OMeClPtCl
Rose Bengal

N

Ciprofloxacin

OCl

CO2H

NNSClMethylene BlueIClO

NONaIZinc Phthalocyanine

Chlorin e6IO

N

CO2HHematoporphyrin

NN

CO2H

CO2HNZn

N

CO2H

N

CO2H

NN

HOOH
CurcuminClNH3NH3Cisplatin 8

Sono-photodynamic Therapy
SPDT✅ High selectivity✅ Low risk of side effects✅ Low permeability✅ High efficiency
USsensitizer

Light

9

Contents• Introduction• Sono-photodynamic Therapy on Cancer• Sono-photodynamic Therapy against Bacteria• Summary

10Cytotoxicity of Ce6
NHN

NH
N

CO2H

N

CO2H

Zinc Phthalocyanine

Chlorin e6OFN
NSClMethylene Blue

CO2H

NNN

HN

Light: 650 nm, 1.2 J/cm22OHO
US: 0.36 W/cm
MeO

NN

CO2H

2HaN

CO2H

PDT: light onlyN
SDT: US only
N SPDT: light → USNN
PSDT:
US → light
Zn

Ciprofloxacin

OMeClPt

HOCl

NH3NH3OHal., Ultrason Sonochem, 2015, 23, 116-127
Wang, P. et

11

Localization

Wang, P. et al., Ultrason Sonochem, 2015, 23, 116-127

12Morphological Analysis

Wang, P. et al., Ultrason Sonochem, 2015, 23, 116-127

13

ROS Detection
DCFH-DAROS

DCFRescue experimentBlack: NACWhite: NAC+Wang, P. et al., Ultrason Sonochem, 2015, 23, 116-127

14Distribution of Ce6Wang, P. et al., Ultrason Sonochem, 2015, 23, 116-127

15

Inhibition of Tumor Growth2h650 nm 1.6 W/cm2Ce63 min
10 mg/kg 120 J/cm23 min

Wang, P. et al., Ultrason Sonochem, 2015, 23, 116-127

16

Apoptosis In Vivo
TUNEL assay

Wang, P. et al., Ultrason Sonochem, 2015, 23, 116-127

17

Decrease of VEGF & MMP-9
IHC assay

VEGF:⾎管新⽣促進遺伝⼦

MMP-9:がん転移促進遺伝⼦
Wang, P. et al., Ultrason Sonochem, 2015, 23, 116-127

18

Short Summary• SPDT is a promising cancer therapy combining
PDT and SDT.
• SPDT is more effective than PDT or SDT alone.
• ROS damage → mitochondria → apoptosis• Decrease in expression level of VEGF andMMP-9

19

Contents• Introduction• Sono-photodynamic Therapy on Cancer• Sono-photodynamic Therapy against Bacteria• Summary

20

Antibacterial Strategies• Antibiotics❌Drug resistance

⇩
• PDT

✅ High selectivity✅ Low risk of side effects✅ Low potential of drug resistance⇩• SDT/SPDT✅ Permeability
21

Nanoparticle

OOHNHNNH
N

CO2H

CO2HHMME

Fe@UCNP-HMME

Wang, Z. et al., Chem. Eng. J., 2019, 356, 811-818

22

1O2 Generation

Light: 980 nm, 1 W/cm2
US: 2 W/cm2

Wang, Z. et al., Chem. Eng. J., 2019, 356, 811-818

23

Bacterial Viability
A: ESBL-producing E. coli
B: MRSAA, B: ESBL-producing E. coliC, D: MRSA

24

Wang, Z. et al., Chem. Eng. J., 2019, 356, 811-818

Contents• Introduction• Sono-photodynamic Therapy on Cancer• Sono-photodynamic Therapy against Bacteria• Summary25

Summary• SPDT is applied to cancer and bacteria
treatment.
• SPDT is more effective than PDT and SDT.
• Mechanism of SPDT remains unclear.

26

APPENDIX

27

1O

2 Quantum Yield

28

ACS Appl. Bio Mater. 2022, 5, 3, 1139–1150.

Metastasis to Lung

29

Imaging

30

Biofilm

31

Biofilm

32