Real-Time PCR

CFX Opus 384 Dx
Real-Time PCR System

Integrated Workflow for the Modern Laboratory
The CFX Opus 384 Dx Real-Time PCR System puts you on the road to anefficient workflow. Quickly set up runs and easily monitor run progress andanalyze data using the comprehensive data management and analysis toolsof CFX Maestro Dx SE Software.
Productivity and Performance■ Create new protocols or quickly upload
existing protocols through the instrument’s
USB port or through your laboratory network
via Ethernet cable
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 rganize and easily find protocols, projects,Oand experiments through the intuitive user
setup and file management structure

CFX Maestro Dx SE Software for theRegulated Laboratory■ Includes integrated security edition feature,removing the need for separate HASP key■■

 ptimize assay development using theO
temperature gradient functionality
 chieve workflow efficiency with its thermal
Aaccuracy and uniformity across the 384-wellsample block
Multiplex up to 4 targets per well
 onnect and control up to 4 CFX Opus
CDx Systems with CFX Maestro Dx
SE Software for Windows 10 (64-bit)Is compatible with laboratory information
management systems
 as application programming interface
Hfor automation readiness as Ethernet connectivity to email
Hcompleted runs

Visit bio-rad.com/CFXOpusDx for more information.

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 ontrols user access by assigning roles
Cand rights
 ssists with U.S. FDA 21 CFR Part 11
A
guidelines by providing audit-ready reports
with run traceability and electronic signaturecapability
 rovides data analysis for multiple experiment
Ptypes, including allelic discrimination,
endpoint, and gene expression
 xports data in multiple formats: CSV, Excel,ERDML, text, XMLCFX Opus 384 Dx Real-Time PCR System

Specifications
SystemLicensed for real-time PCR

Yes

Thermal uniformity	±0.3ºC, measured 10 sec after the block reaches
the target temperature

Sample capacity

384 wells

Gradient

Sample size

1–30 µl (5–30 µl recommended)

Dimensions (W x D x H)		33 x 56 x 36 cm(13 x 22 x 14 in.)

CFX Maestro Dx SE Data Management and Analysis Software

Weight22 kg (48 lb)

Operating system	Windows 10 (64-bit)

Operational range
Programmable span

30–100ºC
1–24ºC

Touch-screen user interface	Adjustable, with angle of rotation 12–55ºMemory

Minimum of 1 GBCommunications

USB 2.0 or above, Ethernet

Data analysis modes

PCR quantification with standard curveElectrical approvals

IEC, CE		

Melt curve analysis

Operating system

Windows 10 IoT		Gene expression analysis by relative quantity (∆Cq)
or normalized expression (∆∆Cq)

Excitation

5 filtered LEDs

Detection

5 filtered photodiodes		Multiple-file gene expression analysis for comparisonof an unlimited number of Cq values

Range of excitation/emission wavelengths

450–690 nm		Allelic discrimination		Endpoint analysis<20 sec
8 sec		Data analysis options include bar charts,
box-and-whisker plots, dot plots, clustergrams,
and scatter plots

Optical Detection System

Scan time
All channels
Single channel
Dynamic range

10 orders of magnitude

Sensitivity 	Detects 1 copy of target sequence in humangenomic DNA
Multiplex analysis

Up to 4 targets per well

384-Well Reaction Block
Sample block type

Fixed 384-well sample block

Heating and cooling method Peltier

Data export	Save, copy, and print all graphs and spreadsheetsfrom right-click menu		

Export specified data in multiple formats		Copy and paste into a Microsoft Excel, Word, orPowerPoint file		Print directly or save as PDFs customizable
reports containing run settings, data graphs, andspreadsheets

Lid heating

30–110ºC

Temperature range

4–100ºC

Maximum ramp rate

2.5ºC/sec

Image export	Export images at any pixel size and at a resolution
up to 600 dpi

Average ramp rate

2.0ºC/sec		

Thermal accuracy±0.2ºC		

Export to RDML

Save images as .bmp, .jpg, or .png files

Ordering Information
Catalog #Description

Visit bio-rad.com/CFXOpusDx for more information.

12014335*
12014348*

CFX Opus 384 Dx Real-Time PCR System, for use in countries
other than China
CFX Opus 384 Dx Real-Time PCR System, for use in China

BIO-RAD, HARD-SHELL, and MICROSEAL are trademarks of Bio-Rad Laboratories, Inc.
in certain jurisdictions. All trademarks used herein are the property of their respective owner.

12014349

CFX Maestro Dx SE Software, English version

12014350

CFX Maestro Dx SE Software, Chinese version

12014351

CFX Maestro Dx SE Software, Russian version

1845099

CFX Qualification Plate, 384-well1814000

PX1 PCR Plate Sealer, includes heat sealing instrument1814030

PCR Plate Heat Seal, pkg of 100, optically clear seals for use
with the PX1 PCR Plate Sealer
Microseal 'B' PCR Plate Sealing Film, pkg of 100, optically clear
seals for PCR plates
Hard-Shell 384-Well PCR Plates, pkg of 50, thin wall, skirted,
clear shell/white wells
Hard-Shell 384-Well PCR Plates, pkg of 50, thin wall, skirted,
clear shell/white wells, barcoded

MSB1001
HSP3805
HSP3905

Hard-Shell Plates are covered by one or more of the following U.S. patents or their foreigncounterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589;
and 6,528,302.
Notice regarding high resolution melt analysis:
No rights are granted by Bio-Rad for the use of high resolution melt analysis in the fields ofhuman or veterinary in vitro diagnostics. In addition, it is the responsibility of the purchaser
to obtain any intellectual property rights which may be required for its specific applications.

* The CFX Opus 384 Dx System ships with power cord, USB cable, Ethernet cable,
and CFX Maestro Dx SE Software, English version.

Bio-RadLaboratories, Inc.
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