RECOMMENDED READING

HAP1 knockout
cell line applicationreferences.

The use of Revvity’s HAP1 cell lines has expanded into a wide
range of reaseach applications such as studies in virology,functional genomics, energy metabolism, and apoptosis,
to name a few. Highlighted below is a list of peer-reviewed
publicaitons that cited Revvity’s HAP1 cell lines along with theircorresponding functional assay methodologies.

Virology
1.

Flint, M. et al. A genome-wide CRISPR screen identifies
N-acetylglucosamine-1-phosphate transferase as a potential
antiviral target for Ebola virus. Nat. Commun. 10, 285 (2019).

2.

Lyoo, H. et al. ACBD3 Is an Essential Pan-enterovirus HostFactor That Mediates the Interaction between Viral 3A
Protein and Cellular Protein PI4KB. mBio 10, e02742-18, /mbio/10/1/mBio.02742-18.atom (2019).

3.

Baggen, J. et al. Bypassing pan-enterovirus host factor
PLA2G16. Nat. Commun. 10, 3171 (2019).

4.

McPhail, J. A. et al. Characterization of the Golgi c10orf76PI4KB complex, and its necessity for Golgi PI4P levels andenterovirus replication. http://biorxiv.org/lookup/doi/10.1101/634592 (2019) doi:10.1101/634592.

5.

Liu, Y. et al. Correction to: Tat expression led to increased
histone 3 tri-methylation at lysine 27 and contributed to HIV
latency in astrocytes through regulation of MeCP2 and Ezh2expression. J. Neurovirol. 25, 901–901 (2019).

HAP1 knockout cell line application references.

6.

7.

8.

Pokharel, S. M. et al. Integrin activation by the lipid

5.

repair factors PAXX, XLF, XRCC4 and DNA-PKcs inproinflammatory response. Nat. Commun. 10, 1482 (2019).

human cells. FEBS Open Bio. 9, 1315–1326 (2019).

Shen, Q. et al. RanBP2/Nup358 enhances miRNA activity

6.

Castañe da-Zegarra, S., Xing, M., Gago-Fuentes, R.,

by sumoylating and stabilizing Argonaute 1.

Sæterstad, S. & Oksenych, V. Synthetic lethality between

https://www.biorxiv.org/content/10.1101/555896v2.

DNA repair factors Xlf and Paxx is rescued by inactivationof Trp53. DNA Repair 73, 164–169 (2019).

LaFontaine, E. et al. Ribosomal protein RACK1 facilitatesefficient translation of poliovirus and other viral IRESs.

9.

Xing, M. & Oksenych, V. Genetic interaction between DNA

molecule 25-hydroxycholesterol induces a7.

Garvin, A. J. et al. The deSUMOylase SENP2 coordinates

http://biorxiv.org/lookup/doi/10.1101/659185 (2019)homologous recombination and nonhomologous end

doi:10.1101/659185.

joining by independent mechanisms.
Genes Dev. 33, 333–347 (2019).

Moskovskich, A. et al. The transporters SLC35A1 andSLC30A1 play opposite roles in cell survival upon VSV

8.

Sarno, A. et al. Uracil–DNA glycosylase UNG1 isoform

virus infection. http://biorxiv.org/lookup/variant supports class switch recombination anddoi/10.1101/573253 (2019) doi:10.1101/573253.

repairs nuclear genomic uracil. Nucleic Acids Res. 47,4569–4585 (2019).

10. Chiramel, A. I. et al. TRIM5α Restricts Flavivirus Replication
by Targeting the Viral Protease for Proteasomal

Methods: G4 DNA staining1; Intron tagging3; survival

Degradation. Cell Rep. 27, 3269-3283.e6 (2019).

assays of NHEJ factors deficient HAP1 cells5; cell cycle
synchronization/staining7.

Methods: CellTiter-Glo viability assays (Promega,Madison, USA)1; reporter virus assays1; virus
quantification (endpoint dilution)2; luciferase reporter

Mitochondria/Energy metabolism

assays8; plaque diameter/size assays8; NFκB
measurement6. Golgi staining4; Viral time-course assays9;

1.

Sánchez-Caballero, L. et al. A dual function of TMEM70 in

Zn-level quantification ; Cell Death/Apoptosis assays ;OXPHOS: assembly of complexes I and V.

IL6 quantification .

http://biorxiv.org/lookup/doi/10.1101/697185 (2019)997

doi:10.1101/697185.

Genome integrity/Functional genomics1.

http://biorxiv.org/lookup/doi/10.1101/654541 (2019)
doi:10.1101/654541.
3.

Yang, Y., Mohammed, F. S., Zhang, N. & Sauve, A. A.
Dihydronicotinamide riboside is a potent NAD +Cui, J., Gizzi, A. & Stivers, J. T. Deoxyuridine in DNA has

concentration enhancer in vitro and in vivo.

an inhibitory and promutagenic effect on RNA

J. Biol. Chem. 294, 9295–9307 (2019).

transcription by diverse RNA polymerases.
Nucleic Acids Res. (2019) doi:10.1093/nar/gkz183.

4.

Małecki, J. M. et al. Human FAM173A is a mitochondrial
lysine-specific methyltransferase that targets adenine

Serebrenik, Y. V., Sansbury, S. E., Kumar, S. S., Henao-nucleotide translocase and affects mitochondrial

Mejia, J. & Shalem, O. Efficient and flexible tagging ofrespiration. J. Biol. Chem. 294, 11654–11664 (2019).

endogenous genes by homology-independent intron
targeting. Genome Res. 29,1322–1328 (2019).
4.

of fusion and fission in a MICOS-dependent manner.

mutational burden is shaped by G4 DNA, replication
Biol. 147, 47–61 (2019).

3.

Kondadi, A. K. et al. Cristae undergo continuous cycles

Bacolla, A., Ye, Z., Ahmed, Z. & Tainer, J. A. Cancer
stress and mitochondrial dysfunction. Prog. Biophys. Mol.

2.

2.

5.

Małecki, J. M. et al. Lysine methylation by themitochondrial methyltransferase FAM173B optimizes

Mair, B. et al. Essential Gene Profiles for Human Pluripotent

the function of mitochondrial ATP synthase.

Stem Cells Identify Uncharacterized Genes and Substrate

J. Biol. Chem. 294, 1128–1141 (2019).

Dependencies. Cell Rep. 27, 599-615.e12 (2019).

www.revvity.com

2HAP1 knockout cell line application references.

6.

Gioran, A. et al. Multi-omics identify xanthine as apro-survival metabolite for nematodes with mitochondrial
dysfunction. EMBO J. 38, (2019).

7.

Drug validation
1.

Drives Cytotoxicity in Both Cancer Cells and Healthy

Guiducci, G. et al. The moonlighting RNA-binding activity

Bone Marrow. Mol. Cancer Res. 17, 409–419 (2019).

of cytosolic serine hydroxymethyltransferase contributes
to control compartmentalization of serine metabolism.

2.

Nucleic Acids Res. 47, 4240–4254 (2019).

tolerance in cancer models: Activity of HDAC6 inhibitors
in cancer models. Int. J. Cancer. 145, 735–747 (2019).

nanoscopy6; respiration measurements bySeahorse 2,4,5,6; NADH measurement3; detection ofcell lysate activity tests of KO/WT cells3.

Methods: drug cytotoxicity assays1; drug target validation2.

Extracellular matrix (ECM)
1.

9, 10611 (2019).

Cao, J. Y. et al. A Genome-wide Haploid Genetic

Methods: whole transcriptome analysis, in vitro

Screen Identifies Regulators of Glutathione Abundance andadhesion assays (e.G. scratch assays).

Ferroptosis Sensitivity. Cell Rep. 26, 1544-1556.e8 (2019).
2.

Lenk, G. M. et al. CRISPR knockout screen
implicates three genes in lysosome function.
Sci. Rep. 9, 9609 (2019).

3.

Ubiquitylation
1.

transcription by opposing PRC1-mediated H2A

with combined immunodeficiency and autoimmune

ubiquitylation. Nat. Commun. 10, 348 (2019).

lysosomal Ca 2+ but evokes Ca 2+ release from the ER
by increasing the cytosolic pH independently of cathepsin
C. J. Cell Sci. 132, jcs223883 (2019).

Epigenetics
1.

Choi, S. et al. H2A.Z-dependent and -independent
recruitment of metabolic enzymes to chromatin required

Agrotis, A., Pengo, N., Burden, J. J. & Ketteler, R.

for histone modifications. http://biorxiv.org/lookup/Redundancy of human ATG4 protease isoforms in

doi/10.1101/553297 (2019) doi:10.1101/553297.

autophagy and LC3/GABARAP processing revealed in

6.

Methods: chromatography analysis of nuclear extracts.

Atakpa, P., van Marrewijk, L. M., Apta-Smith, M.,
Chakraborty, S. & Taylor, C. W. GPN does not release5.

Campagne, A. et al. BAP1 complex promotes

Keskitalo, S. et al. Dominant TOM1 mutation associateddisease. Npj Genomic Med. 4, 14 (2019).

4.

Blum, A. et al. Transcriptomics of a KDELR1 knockout cellline reveals modulated cell adhesion properties. Sci. Rep.

Autophagy/Apoptosis
1.

Depetter, Y. et al. Selective pharmacological inhibitors ofHDAC6 reveal biochemical activity but functional

Methods: electron microscopy2, super-resolution

respiratory chain complexes (MS/MS analysis)1,4;
Pulse labelling of mitochondrial translation products1;

Hopkins, T. A. et al. PARP1 Trapping by PARP Inhibitors

cells. Autophagy 15, 976–997 (2019).

Methods: cellular extraction in cytosol, mitochondrial

Simons, I. M. et al. The highly GABARAP specific rat

protein fractions.

and membrane, nuclear, and chromatin-boundmonoclonal antibody 8H5 visualizes GABARAP in
immunofluorescence imaging at endogenous levels.
Sci. Rep. 9, 526 (2019).
Methods: human haploid cell genetic screening1;
glutathione (GSH) quantification via monochlorobimane(MCB) GSH probes/FACS Analysis and Ellman’s reagent1;
immuno-staining via autophagy marker (e.g.LC3 puncta)5;transmission electron microscopy (TEM)5.
www.revvity.com

3HAP1 knockout cell line application references.

Translational apparatus
1.

Vindry, C., Guillin, O., Mangeot, P. E., Ohlmann,
T. & Chavatte, L. A Versatile Strategy to Reduce UGA-

3.

bleb retraction. Mol. Biol. Cell 30, 1051–1059 (2019).
4.

lookup/doi/10.1101/635615 (2019) doi:10.1101/635615.

CRISPR-Cas9-Viral-Like-Particles Targeting2.

5.

Tuladhar, R. et al. Stereoselective fatty acylation isessential for the release of lipidated WNT proteins fromHenriques, S. F., Gicquel, E., Marsolier, J. & Richard, I.

the acyltransferase Porcupine (PORCN). J. Biol. Chem.

Functional and cellular localization diversity associated294, 6273–6282 (2019).

with Fukutin-related protein patient genetic variants.
Hum. Mutat. 40, 1874–1885 (2019).

Taneja, N. et al. Precise tuning of cortical contractility
regulates cell shape during cytokinesis. http://biorxiv.org/

Selenocysteine Recoding Efficiency of the Ribosome Using
Selenocysteine-tRNA[Ser]Sec Gene. Cells 8, 574 (2019).

Taneja, N. & Burnette, D. T. Myosin IIA drives membrane

6.

Methods: Northern blot analysis1, tRNA transduction1;
TIDE analysis1.

Karsai, G. et al. DEGS1-associated aberrant sphingolipid
metabolism impairs nervous system function in humans.
J. Clin. Invest. 129, 1229–1239 (2019).
Methods: differential Interference Contrast (DIC)
microscopy and Fluorescence Recovery After

Cell biology
1.

Photobleaching (FRAP)4; WNT secretion assay (cdDNA

Nixon, A. M. L. et al. A rapid in vitro methodology for
simultaneous target discovery and antibody
generation against functional cell subpopulations.

transfection, ConA–Sepharose affinity chromatography
and Western Blot Analysis)5; metabolite labelingand detection6.

Sci. Rep. 9, 842 (2019).
2.

Sergeeva, O. A. & van der Goot, F. G. Anthrax toxin
requires ZDHHC5- mediated palmitoylation of its surfaceprocessing host enzymes. Proc. Natl. Acad. Sci. 116,1279–1288 (2019).

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