Real-Time PCR

CFX Opus Deepwell Dx
Real-Time PCR System

Reliable Patient Results with Large Reaction Volumes
The CFX Opus Deepwell Dx Real-Time PCR System provides a robust
and reliable open platform for development and performance of in vitro
diagnostic assays. The Deepwell format allows large reaction volumes(up to 125 µl) for sample matrices and workflows that require it. When usedwith CFX Maestro Dx SE Software for security and traceability, the integrityof patient results is ensured. Quickly set up runs and easily monitor run
progress and analyze data using the comprehensive data managementand analysis tools of CFX Maestro Dx SE Software.
Benefits■ 
One of the industry’s largest real-time
PCR reaction volumes, up to 125 µl
■

■

■

■

■

■

■

 ssist with U.S. FDA 21 CFR Part 11A
regulations using CFX Maestro Dx SE
Software security, audit, and electronic
signature capabilities
 iew, sign, save, and print an audit report
Vwith CFX Maestro Dx SE Software ontrol access to protocols and run files
Con the instrument with User Management
through use of User Name and password
 tilize thermal gradient function to assistUwith assay optimization during development
 ontrol up to four CFX Opus Dx Systems
Cwith CFX Maestro Dx SE Software forWindows 10 (64-bit)
Email your completed run ave protocols and completed runs inSa network folder

Visit bio-rad.com/CFXOpusDx for more information.

The system’s intuitive touch-screen userinterface supports both experienced and new
real-time PCR users.
■■ reate new real-time PCR protocols or easilyC
modify existing protocols
 et up unique User Names, with optional
Spasswords, to easily organize and find yourprotocols and experimentsCFX Opus Deepwell Dx Real-Time PCR System

Specifications
SystemLicensed for real-time PCR
Yes
Sample capacity
96 wells
Sample size
1–125 µl (10–125 µl recommended)
Dimensions (W x D x H)
33 x 56 x 36 cm		(13 x 22 x 14 in.)
Weight22 kg (48 lb)
Touch-screen user interface	Adjustable, with angle of rotation 12–55ºCommunications
USB 2.0 or above, Ethernet
Electrical approvals
IEC, CE
Operating system
Windows 10 IoT
Optical Detection System
Excitation
Detection
Range of excitation/emission wavelengths
Scan time
All channels
Single channel
Dynamic rangeSensitivity		
Multiplex analysis

6 filtered LEDs
6 filtered photodiodes
450–730 nm

12 sec3 sec
10 orders of magnitude
Detects 1 copy of target sequencein human genomic DNA
Up to 5 targets per well

96-Well Reaction Block
Sample block type
Fixed 96-well sample block
Heating and cooling method Peltier
Lid heating
30–110ºC
Temperature range
4–100ºC
Maximum ramp rate
2.5ºC/sec
Average ramp rate
2ºC/sec
Thermal accuracy±0.2ºC
Thermal uniformity	± 0.4ºC, measured 10 sec after the block
reaches the target temperature

GradientOperational range		Programmable span

30–100ºC
1–24ºC

CFX Maestro Dx SE Data Management and Analysis Software
Operating system	Windows 10 (64-bit)
MemoryMinimum of 1 GB

Data analysis modes

PCR quantification with standard curve		

Melt curve analysis		Gene expression analysis by relative quantity (∆Cq)
or normalized expression (∆∆Cq)
		Multiple-file gene expression analysis for comparisonof an unlimited number of Cq values		

Allelic discrimination		Endpoint analysis		Data analysis options include bar charts,
box-and-whisker plots, dot plots, clustergrams,
and scatter plots
Data export	Save, copy, and print all graphs and spreadsheetsfrom right-click menu		

Export specified data in multiple formats		Copy and paste into a Microsoft Excel, Word, orPowerPoint file		Customizable reports containing run settings, datagraphs, and spreadsheets can be printed directly or
saved as PDFs		

Export to RDML

Image export	Export images at any pixel size and at a resolution
up to 600 DPI		

Save images as .bmp, .jpg, or .png files

Ordering Information
Catalog #Description

12016687*
12016659*

CFX Opus Deepwell Dx Real-Time PCR System, for use incountries other than China
CFX Opus Deepwell Dx Real-Time PCR System, for use in China

12014349

CFX Maestro Dx SE Software, English version

12014350

CFX Maestro Dx SE Software, Chinese version

12014351

CFX Maestro Dx SE Software, Russian version

1845098

CFX Qualification Plate, 96-well1814000

PX1 PCR Plate Sealer, includes heat sealing instrument

1814030

PCR Plate Heat Seal, pkg of 100, optically clear seals for use with
the PX1 PCR Plate Sealer
Microseal™ 'B' PCR Plate Sealing Film, pkg of 100, optically clearseals for PCR plates
Hard-Shell™ 96-Well PCR Plates, pkg of 50, low profile, thin wall,skirted, white shell/white wells
Hard-Shell 96-Well PCR Plates, pkg of 50, low profile, thin wall,skirted, white shell/white wells, barcoded

MSB1001
HSP9655
HSP9955

Visit bio-rad.com/CFXOpusDx for more information.
BIO-RAD, HARD-SHELL, and MICROSEAL are trademarks of Bio-Rad Laboratories, Inc.
in certain jurisdictions. All trademarks used herein are the property of their respective owner.
© 2022 Bio-Rad Laboratories, Inc.
Notice regarding high resolution melt analysis:
No rights are granted by Bio-Rad for the use of high resolution melt analysis in the fields ofhuman or veterinary in vitro diagnostics. In addition, it is the responsibility of the purchaser
to obtain any intellectual property rights which may be required for its specific applications.

* The CFX Opus Deepwell Dx System ships with power cord, USB cable,
Ethernet cable, and CFX Maestro Dx SE Software, English version.

Bio-RadLaboratories, Inc.
Website bio-rad.com USA 1 800 424 6723 Australia 61 2 9914 2800 Austria 00 800 00 24 67 23 Belgium 00 800 00 24 67 23 Brazil 4003 0399
Canada 1 905 364 3435 China 86 21 6169 8500 Czech Republic 00 800 00 24 67 23 Denmark 00 800 00 24 67 23 Finland 00 800 00 24 67 23France 00 800 00 24 67 23 Germany 00 800 00 24 67 23 Hong Kong 852 2789 3300 Hungary 00 800 00 24 67 23 India 91 124 4029300 Israel 0 3 9636050
Italy 00 800 00 24 67 23 Japan 81 3 6361 7000 Korea 82 2 3473 4460 Luxembourg 00 800 00 24 67 23 Mexico 52 555 488 7670
The Netherlands 00 800 00 24 67 23 New Zealand 64 9 415 2280 Norway 00 800 00 24 67 23 Poland 00 800 00 24 67 23 Portugal 00 800 00 24 67 23
Russian Federation 00 800 00 24 67 23 Singapore 65 6415 3188 South Africa 00 800 00 24 67 23 Spain 00 800 00 24 67 23 Sweden 00 800 00 24 67 23
Switzerland 00 800 00 24 67 23 Taiwan 886 2 2578 7189 Thailand 66 2 651 8311 United Arab Emirates 36 1 459 6150 United Kingdom 00 800 00 24 67 23Life ScienceGroup

Bulletin 3402 Ver AUS/EG22-04780822

Sig 0122