Histone H3K27me3 antibody (mAb)
Catalog Nos: 61017, 61018
RRID: AB_2614987
Clone: MABI 0323
Application(s): ChIP, ChIP-Seq, DB, ICC, IF, WB
Reactivity: Human, Mouse, Wide Range Predicted

Quantities: 100 µg, 50 µg
Purification: Protein G Chromatography
Host: Mouse
Isotype: IgG1
Molecular Weight: 17 kDa

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit ofchromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of HistoneH2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between thenucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.
Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins
and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation,phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play amajor role in regulating gene expression.
The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated withtranscriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- ortrimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone
methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.
Immunogen: This Histone H3 trimethyl Lys27 antibody was raised against a peptide containing trimethyl Lys27 of human
Histone H3.
Buffer: PBS pH 7.5 containing 30% glycerol. 0.3M NaCl, and 0.035% sodium azide. Sodium azide is highly toxic.
Application Notes:
Applications Validated by Active Motif:
WB: 0.5 - 2 µg/ml dilution
DB: 0.5 - 2 µg/ml dilution
CUT&Tag: 1 µg per 50 µl reaction
Storage and Guarantee: Some products may be shipped at room temperature. This will not affect their stability orperformance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2years. Keep all reagents on ice when not in storage. This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
This antibody is manufactured by MAB Institute, Inc.

Application Key: ChIP = Chromatin Immunoprecipitation; FACS = Flow Cytometry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; WB = Western Blot

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Western blot of Histone H3 trimethyl Lys27 antibody.
HeLa nuclear extract (20 µg per lane) probed with Histone H3 trimethyl Lys27 antibody (2 µg/mldilution).

Dot blot of Histone H3 trimethyl Lys27 antibody.
Dot blot analysis was used to confirm the specificity of Histone H3 trimethyl Lys27 antibody fortrimethyl Lys27 of histone H3. Recombinant methylated histone proteins corresponding to theimmunogen and related sequences were spotted onto PVDF and probed with Histone H3trimethyl Lys27 at 2 µg/ml. The amount of protein (picomoles) spotted is indicated next to eachrow. Lane 1: unmodified H3 protein. Lane 2: monomethyl Lys4 protein. Lane 3: dimethyl Lys4protein. Lane 4: trimethyl Lys4 protein. Lane 5: monomethyl Lys9 protein. Lane 6: dimethyl Lys9protein. Lane 7: trimethyl Lys9 protein. Lane 8: monomethyl Lys27 protein. Lane 9: dimethyl
Lys27 protein. Lane 10: trimethyl Lys27 protein.

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