Handling Instructions 3D Serpentine Mixer
Fluidic 1079

Chip description
The 3D serpentine mixer chip Fluidic 1079 possesses four
identical micromixing units. Each unit features three inlet
ports and one outlet port. Those ports come in Mini Luer
format. The mixing channel is characterized by a sequenceof directional changes in all three dimensions. It therefore
possesses three different types of channel sections that are:1.) horizontal, located on the bottom side of the chip,2.) horizontal, located on the top side of the chip, and3.) vertical, connecting the two horizontal channels.

Effective micromixing of up to three fluids
in a 3D serpentine channel

Channel features of the mixing units are:• Channel depth horizontal channels: 200 µm• Channel width horizontal channels: 500 µm• Channel diameter vertical channel: 800 µm• Total channel volume: 12.93 µl• Total channel length: 60 mm• Directional changes per mixing unit: 10

Many microfluidic systems require rapid mixing of different fluids within microchannels. While diffusion can be an effectivemode of mixing in microchannels with predominantly laminarflows, it often requires prolonged mixing times and/or ideally
increase of interfacial area.
Introducing chaotic (but still laminar) flow patterns can aid mixing efficiency in microchannels. This strategy becomes evenmore important when mixing solutions with particularly low
diffusion coefficients.
The 3D serpentine mixer has been developed especially toaddress this challenge in microfluidics. It is the perfect tool
to investigate fast and efficient passive micromixing of up tothree liquids.
Contact in Japan: ASICON Tokyo Ltd.
www.asicon-tokyo.com / asicon-tokyo@asicon-tokyo.com

Handling Instructions Fluidic 1079
Handling instructions - a step-by-step description
Horizontal channel, chip bottom

Inlet channel 1

Inlets

Inlet channel 2
Inlet channel 3

1
2

Horizontal channel, chip top
Vertical channel

Outlet3

Necessary equipment• 1 x chip Fluidic 1079
• 3 pcs male Mini Luer Fluid connectors• 1 pc male Mini Luer plug
• Silicon sleeve (cut from silicone tube)• PTFE tubing• Handling frame• Pump system of your choice (e.g. pressure-driven pumpwith two channels)

Step 1• Place chip into the handling frame• Connect Mini Luer connectors with silicone sleeves• Insert PTFE tubing into silicone sleeves• Insert Mini Luer connectors into Mini Luer interfaces(here: blue - inlets; yellow - outlet)• Close unused Mini Luer ports with Mini Luer plug (red)• Connect PTFE tubing with pumps

Step 2• Connect further chip modules to your setup, e.g.

channel or chamber chips (optional)• Start micropumps to pump fluids to be mixed• Use low flow rates (e.g. 5 µl/min for each channel)• Adjust flow rates depending on fluids to achievecomplete mixing within microchannel

Handling Instructions Fluidic 1079

Application example: mixing of colored water
Here we show exemplary how flow speeds of the two liquids to be mixed, influence mixing results. The 3D serpentine mixer wasused to mix yellow and blue colored water. Two fluid streams were used in this example application, while up to three different
fluids can be mixed with the 3D serpentine mixer.
The mixing units of Fluidic 1079 can also be daisy-chained in order to increase mixing efficiency.

3+3 µl / min
Mixing at relatively low flow rates (each channel 3 µl /min) of yellow and blue fluid streams

5+10 µl / min
The 3D serpentine mixer is well suited to mix fluid streams withdifferent flow rates (yellow 10 µl / min; blue 5 µl / min)20+20 µl / min
Mixing at relatively high flow rates (each channel 20 µl /min) ofyellow and blue fluid streams

Off-the-shelf available - 3D serpentine mixer chip Fluidic 1079
The 3D serpentine mixer chip Fluidic 1079 is off-the-shelf available in two different thermoplastic polymers, which are Zeonor(COP) and Polycarbonate (PC).

3D serpentine mixer chip with four functional mixing units andMini Luer interfaces

Detail of serpentine channel of one mixing unit of Fluidic 1079

Assembly of Mini Luer fluid connector, silicone sleeve and PTFE
tubingDescription

Material

Lid
Thickness [µm]10001477

3D serpentine mixerZeonor

188

10001480

3D serpentine mixer

PC175

Product Codefor Fluidic 1079

Product CodeDescription of Accessories

Material

Quantity

10000096

Male Mini Luer fluid connector

PP - Blue

10 pcs / pack

10000280

Male Mini Luer plugs – Low volume displacement

PP - Red

10 pcs / pack10000031

Silicone tube, ID: 0.76 mm, OD: 1.65 mm

Silicone

1m10000032

Micro tubes, PTFE, ID: 0.5 mm, OD:1.0 mm

PTFE1mContact in Japan: ASICON Tokyo Ltd.
www.asicon-tokyo.com / asicon-tokyo@asicon-tokyo.com