Publications
1.

Wakayama T, Maruyama Y, Imamura K, and Fukuta K. (1993) Penetration of the Zona
Pellucida of Eggs of Different Species by Spermatozoa of the Japanese Field Vole, Microtus

montebelli. J. Reprod. Dev. 39:319-323.
2.
Wakayama T, Matsubara Y, Imamura K, Kurohmaru M, Hayashi Y, and Fukuta K. (1994)
Development of early-stage embryos of the Japanese field vole, Microtus montebelli, in vivo and invitro. J. Reprod. Fertil. 101:663-666.
3.
Wakayama T, Tanemura K, Suto J, Imamura K, Fukuta K, Mori H, Kuramoto K, Kurohmaru M,
and Hayashi Y. (1995) Production of term offspring by in vitro fertilization using old mouse
spermatozoa. J. Vet. Med. Sci. 57:545-547.
4.
Suto J, Wakayama T, Imamura K, Goto S, and Fukuta K. (1995) Incomplete development of thespleen and the deformity in the chimeras between asplenic mutant (Dominant hemimelia) andnormal mice. Teratology. 52: 71-77.
5.
Wakayama T, Suto J, Matsubara Y, Imamura K, Fukuta K, Toyoda Y, Kurohmaru M, andHayashi Y. (1995) In vitro fertilization and embryo development of Japanese Field Vole, Microtus
montebelli. J. Reprod. Fertil. 104: 63-68.
6.
Endo H, Hondo E, Yamagiwa D, Wakayama T, Kurohmaru, M, and Hayashi Y. (1996)
Distribution of cardiac musculature in the pulmonary venous wall of three species of the genus
Mustela. Mammal Study 21: 37-42.
7.
Ogura A, Yamamoto Y, Matsuda J, Suzuki O, Wakayama T, Kimura H, and Asano T. (1996) In
vitro fertilization and microinsemination with round spermatids for propagation of nephrotic genes
in mice. Theriogenology 45: 1141-1149.
8.
Suto J, Wakayama T, Imamura K, Goto S, and Fukuta K. (1996) Observation on the skeletal
malformations caused by the Dh (Dominant hemimelia) gene in mice. Exp. Anim. 45:95-98.
9.
Suto J, Wakayama T, Imamura K, Goto S, and Fukuta K. (1996) High lethality of F1 male Dh/+mice from the cross between female DDD and male Dh/+. Exp. Anim. 45:99-101.
10.
Wakayama T, Suto J, Imamura K, Toyoda Y, Kurohmaru, M, and Hayashi Y. (1996) Effect ofhypotaurine on In vitro fertilization and production of term offspring from in vitro fertilized ova of
the Japanese Field Vole, Microtus montebelli. Biol. Reprod. 54: 625-630.
11.
Wakayama T, Ogura A, Suto J, Matsubara Y, Kurohmaru M, Hayashi Y, and Yanagimachi R.
(1996) Penetration by field vole spermatozoa of mouse and hamster zonae pellucidae without
acrosome reaction. J. Reprod. Fertil. 107: 97-102.
12.
Tanemura K, Wakayama T, Kuramoto K, Hayashi Y, and Ogura A. (1997) Birth of normal
young by microinsemination with frozen-thawed round spermatids collected from aged azoospermic
mice. Lab. Anim. Sci. 47: 203-204.

1

13.

Wakayama T, Hayashi Y, and Ogura A. (1997) Participation of the female pronucleus derived

from the second polar body in full embryonic development of mice. J. Reprod. Fertil. 110:263-266.
14.
Ogura A, Wakayama T, Suzuki O, Shin T.Y, Matsuda J, and Kobayashi Y. (1997)
Chromosomes of mouse primary spermatocytes undergo meiotic divisions after incorporation intohomologous immature oocytes. Zygote. 5: 177-182.
15.
Wakayama T, Uehara T, Hayashi Y, and Yanagimachi R. (1997) The response of mouse
oocytes injected with sea urchin spermatozoa. Zygote. 5: 229-234.
16.
Cummins J.M, Wakayama T, and Yanagimachi R. (1997) Fate of microinjected spermcomponents in the mouse oocyte and embryo. Zygote. 5: 301-308.
17.
Wakayama T, Whittengham D.G, and Yanagimachi R. (1998) Production of normal offspringfrom mouse oocytes injected with spermatozoa cryopreserved with or without cryoprotection. J.
Reprod. Fertil. 112:11-17.
18.
Dozortsev D, Wakayama T, Ermilov A, and Yanagimachi R. (1998) Intracytoplasmic sperm
injection in the rat. Zygote. 6:143-147.
19.
Cummins J.M, Wakayama T, and Yanagimachi R. (1998) Fate of microinjected immature sperm
mitochondria in the mouse oocyte and embryo. Zygote. 6:213-222.
20.
Tateno H, Wakayama T, Ward W. S, and Yanagimachi R. (1998) Can alcohol retain the
reproductive and genetic potential of sperm nuclei?: chromosome analysis os mouse spermatozoa
stored in alcohol. Zygote. 6:233-238.
21.
Wakayama T, and Yanagimachi R. (1998) Fertilizability and developmental ability of mouse
oocytes with reduced amount of cytoplasm. Zygote 6:341-346.
22.
Wakayama T, and Yanagimachi R. (1998) The first polar body can be used the production of
normal offspring. Biol. Reprod. 59:100-104.
23.
Wakayama T, and Yanagimachi R. (1998) Development of normal mice from oocytes injectedwith freeze-dried spermatozoa. Nat. Biotechnol.16:639-641. (Cover article of issue).
24.
Wakayama T, Perry ACF, Zuccotti M, Johnson KR, and Yanagimachi R. (1998) Full term
development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature
394:369-374. (Cover article of issue).
25.

Perry ACF, Wakayama T, and Yanagimachi R. (1999) A novel trans-complementation assay
suggests full mammalian oocyte activation is co-ordinately initiated by multiple, sub-membrane

sperm components. Biol. Repord. 60:747-755.
26.
Perry ACF, Wakayama T, Kishikawa H, Kasai T, Okabe M, Toyoda Y, and Yanagimachi R.
(1999) Mammalian transgenesis by intracytoplasmic sperm injection. Science 284: 1180-1183.
27.

Wakayama T, and Yanagimachi R. (1999) Cloning of male mice from adult tail-tip cells. Nat.
Genet. 22:127-128.
2

28.

Wakayama T, Rodriguez I, Perry ACF, Yanagimachi R, and Mombaerts P. (1999) Mice cloned

from embryonic stem cells. Proc. Natl. Acad. Sci. USA 26:14984-14989.
29.
Kishikawa H, Wakayama T, and Yanagimachi R. (1999) Comparison of oocyte-activating
agents for mouse cloning. Cloning 1:153-159.
30.
Perry ACF, Wakayama T , Cooke IM, and Yanagimachi R. (2000) Mammalian oocyte activationby the synergistic action of discrete sperm head components:Induction of calcium transients andinvolvement of proteolysis. Dev. Biol. 217: 386-393.
31.
Wakayama T, Tateno H, Mombaerts P, and Yanagimachi R. (2000) Nuclear transfer into mousezygotes. Nat. Genet. 24:108-109.
32.
Rideout III W. M, Wakayama T, Wutz A, Eggan K, Jackson-Grusby L, Dausman J,
Yanagimachi R, and Jaenisch R. (2000) Generation of mice from wild-type and targeted ES cells by
nuclear cloning. Nat. Genet. 24:109-110.
33.
Tamashiro KLK, Wakayama T, Blanchard RJ, Blanchard DC, and Yanagimachi R. (2000)
Postnatal growth and behavioral development of mice cloned from adult cumulus cells. Biol.
Reprod. 63:328-334.
34.
Ogura A, Inoue K, Takano K, Wakayama T, and Yanagimachi R. (2000) Birth of Mice after
Nuclear Transfer by Electrofusion using Tail Tip Cells. Mol. Reprod. Dev. 57:55-59.
35.
Wakayama T, Shinkai Y, Tamashiro KLK, Niida H, Blanchard DC, Blanchard RJ, Ogura A,
Tanemura K, Tachibana M, Perry ACF, Colgan DF, Mombaerts P, and Yanagimachi R. (2000)
Cloning of mice to six generations. Nature 407: 318-319.
36.
Wakayama T, and Yanagimachi R. (2001) Mouse cloning with nucleus donor cells of different
age and type. Mol. Reprod. Dev. 58: 376-383.
37.
Wakayama T, and Yanagimachi R. (2001) Effect of cytokinesis inhibitor, DMSO and the timingof oocyte activation on mouse cloning using cumulus cell nuclei. Reproduction 122:49-60
38.
Wakayama T, Tabar V, Rodriguez I, Perry ACF, Studer L, and Mombaerts P. (2001)
Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer.
Science. 292:740-743.
39.
Ohgane J, Wakayama T, Kogo Y, Senda S, Hattori N, Tanaka S, Yanagimachi R, and Shiota K.
(2001) DNA methylation variation in cloned mice. Genesis 30:45-50.
40.
Kawase Y, Iwata T, Toyoda Y, Wakayama T, Yanagimachi R, and Suzuki H (2001) Comparisonof intracytoplasmic sperm injection for inbred and hybrid mice Mol. Reprod. Dev. 60:74-78.
41.
Perry ACF, Rothman A, de Las Heras JI, Feinstein P, Mombaerts P, Cooke HJ, and Wakayama
T. (2001) Efficient metaphase II transgenesis with different transgene archetypes. Nat. Biotechnol.
19:1071-1073.

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42.

Tanaka S, Oda M, Toyoshima Y, Wakayama T, Tanaka M, Yoshida N, Hattori N, Ohgane J,
Yanagimachi R, and Shiota K (2001) Placentomegaly in cloned mouse concepti caused byexpansion of the spongiotrophoblast layer. Biol Reprod. 65:1813-1821.
43.
Yamazaki Y, Wakayama T, and Yanagimachi R. (2001) Contribution of cumulus cells andserum to the maturation of oocyte cytoplasm as revealed by intracytoplasmic sperm injection (ICSI).
Zygote 2001 9:277-282.
44.
Tamashiro KL, Wakayama T, Akutsu H, Yamazaki Y, Lachey JL, Wortman MD, Seeley RJ,
D'Alessio DA, Woods SC, Yanagimachi R, and Sakai RR. (2002) Cloned mice have an obese
phenotype not transmitted to their offspring. Nat. Med 8:262-267 (Cover article of issue).
45.

Wakayama S, Cibelli JB, and Wakayama T. (2003) Effect of timing of the removal of oocyte

chromosomes before or after injection of somatic nucleus on development of NT embryos. Cloning
Stem Cells 5: 181-189.
46.
Singh U, Fohn LE, Wakayama T, Ohgane J, Steinhoff C, Lipkowitz B, Schulz R, Orth A, Ropers
HH, Behringer RR, Tanaka S, Shiota K, Yanagimachi R, Nuber UA, and Fundele R. (2004)
Different molecular mechanisms underlie placental overgrowth phenotypes caused by interspecies
hybridization, cloning, and Esx1 mutation. Dev. Dyn. 230, 149-164.
47.
Yanagimachi R, Wakayama T, Kishikawa H, Fimia GM, Monaco L, and Sassone-Corsi P.
(2004) Production of fertile offspring from genetically infertile male mice. Proc. Natl. Acad. Sci.
USA 101, 1691-1695.
48.
Ohgane J, Wakayama T, Senda S, Yamazaki Y, Inoue K, Ogura A, Marh J, Tanaka S,
Yanagimachi R, and Shiota K. (2004) The Sall3 locus is an epigenetic hotspot of aberrant DNA
methylation associated with placentomegaly of cloned mice. Genes Cells. 9:253-260.
49.
Lanza R, Moore MA, Wakayama T, Perry AC, Shieh JH, Hendrikx J, Leri A, Chimenti S,
Monsen A, Nurzynska D, West MD, Kajstura J, and Anversa P. (2004) Regeneration of the Infarcted
Heart With Stem Cells Derived by Nuclear Transplantation. Circulation Research. 94:820-827.
50.
Kishigami S, Wakayama S, Nguyen VT, and Wakayama T. (2004) Similar time restriction for
ICSI and ROSI into activated oocytes for efficient offspring production Biol Reprod. 70:1863-1869
51.
Ohta H, Wakayama T, and Nishimune Y. (2004) Commitment of Fetal Male Germ Cells to
Spermatogonial Stem Cells During Mouse Embryonic Development. Biol Reprod. 70: 1286-1291
52.
Ohta H, and Wakayama T. (2004) Full-term development of offspring using ectopically
produced haploid germ cells from fetal male germ cells. J. Reprod. Dev. 50:429-437.
53.
Senda S, Wakayama T, Yamazaki Y, Ohgane J, Hattori N, Tanaka S, Yanagimachi R, and Shiota
K. (2004) Skewed X-inactivation in cloned mice. Biochem Biophys Res Commun. 321:38-44.
54.
Wakayama S, Thuan NV, Kishigami S, Ohta H, Mizutani E, Hikichi T, Miyake M, andWakayama T. (2004) Production of offspring from one-day-old oocytes stored at room temperature.
J. Reprod. Dev. 50:627-637.
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55.

Kishigami S, Thuan NV, Wakayama S, Hikichi T, and Wakayama T. (2004) A new separation

method of the round-spermatid cytoplasm before ROSI in mouse. Zygote 12: 321-327
56.
Mochida K, Wakayama T, Takano K, Noguchi Y, Yamamoto Y, Suzuki O, Matsuda J, andOgura A. (2005) Birth of Offspring after Transfer of Mongolian Gerbil (Meriones unguiculatus)
Embryos Cryopreserved by Vitrification. Mol. Reprod. Dev. 70: 464-470.
57.
Van Thuan N, Wakayama S, Kishigami S, and Wakayama T (2005) New Preservation Method
for Mouse Spermatozoa Without Freezing. Biol. Reprod. 72, 444–450.
58.
Hikichi T, Kishigami S, Thuan NV, Ohta H, Mizutani E, Wakayama S, and Wakayama T.
(2005) Round spermatids stained with mitotracker can be used to produce offspring more simply.
Zygote. 2005 13:55-61.
59.
Wakayama S, Ohta H, Kishigami S, Van Thuan N, Hikichi T, Mizutani E, Miyake M, andWakayama T. (2005) Establishment of Male and Female Nuclear Transfer Embryonic Stem Cell
Lines from Different Mouse Strains and Tissues. Biol. Reprod. 72, 932-936.
60.
Wakayama S, Kishigami S, Van Thuan N, Ohta H, Hikichi T, Mizutani E, Yanagimachi R, andWakayama T. (2005) Propagation of an Infertile Hermaphrodite Mouse Lacking Germ Cells, Using
Nuclear Transfer and Embryonic Stem Cell Technology. Proc. Natl. Acad. Sci. USA 102:29-33.
(Cover article of issue)
61.

Ohta H, and Wakayama T. (2005) Generation of Normal Progeny by Intracytoplasmic Sperm

Injection Following Grafting of Testicular Tissue from Cloned Mice that Died Postnatally. Biol
Reprod. 73:390-395
62.
Fujita K, Ohta H, Tsujimura A, Takao T, Miyagawa Y, Takada S, Matsumiya K, Wakayama T,
and Okuyama A. (2005) Transplantation of spermatogonial stem cells isolated from leukemic mice
restores fertility without inducing leukemia. J .Clin. Invest. 115:1855-1861
63.
Mizutani E, Ohta H, Kishigami S, Van Thuan N, Hikichi T, Wakayama S, Sato E, andWakayama T. (2005) Generation of progeny from embryonic stem cells by microinsemination ofmale germ cells from chimeric mice. Genesis. 43:34-42.
64.
Singh U, Sun T, Shi W, Schulz R, Nuber UA, Varanou A, Hemberger MC, Elliott RW, Ohta H,
Wakayama T, and Fundele R. (2005) Expression and Functional Analysis of Genes Deregulated in
Mouse Placental Over Growth Models: Car2 and Ncam1. Dev. Dyn. 234:1034-1045
65.
Wakayama S, Mizutani E, Kishigami S, Thuan NV, Ohta H, Hikichi T, Bui HT, Miyake M, andWakayama T. (2005) Mice Cloned by Nuclear Transfer from Somatic and ntES Cells Derived fromthe Same Individuals. J. Reprod. Dev. 51:765-72.
66.
Kishigami S, Thuan NV, Hikichi T, Ohta H, Wakayama S, Mizutani E, and Wakayama T.
(2006) Epigenetic abnormalities of the mouse paternal zygotic genome associated withmicroinsemination of round spermatids. Dev. Biol 289: 195-205.

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67.

Tamada H , Van Thuan N, Reed P, Nelson D, Katoku-Kikyo N, Wudel J, Wakayama T, and N.
Kikyo N. (2006) Chromatin decondensation and nuclear reprogramming by nucleoplasmin, Mol

Cell Biol, 26: 1259-1271.
68.
Kishigami S, Mizutani E, Ohta H, Hikichi T, Thuan NV, Wakayama S, Bui HT, and Wakayama
T. (2006) Significant improvement of mouse cloning technique by treatment with trichostatin A after
somatic nuclear transfer. Biochem. Biophys. Res. Commun. 340: 183-189.
69.
Van Thuan N, Wakayama S, Kishigami S, and Wakayama T. (2006) Donor Centrosome
Regulation of Initial Spindle Formation in Mouse Somatic Cell Nuclear Transfer: Roles ofGamma-Tubulin and Nuclear Mitotic Apparatus Protein 1. Biol. Reprod. 74:777-787.
70.
Van Thuan, N, Wakayama S, Kishigami S, Ohta H, Hikichi H, Mizutani E, Bui HT, andWakayama T. (2006) Injection of Somatic Cell Cytoplasm into Oocytes Before ICSI Impairs
Full-Term Development and Increases Placental Weight in Mice. Biol. Reprod. 74:865-873.
71.
Kishigami S, Hikichi T, Van Thuan N, Ohta H, Wakayama S, Bui HT, Mizutani E, andWakayama T. (2006) Normal specification of the extraembryonic lineage after somatic nuclear
transfer. FEBS Lett. 580:1801-1806
72.
Wakayama S, Jakt ML, Suzuki M, Araki R, Hikichi T, Kishigami S, Ohta H, Thuan NV,
Mizutani E, Sakaide Y, Senda S, Tanaka S, Okada M, Miyake M, Abe M, Nishikawa SI, Shiota K,
and Wakayama T. (2006) Equivalency of Nuclear Transfer-Derived Embryonic Stem Cells to those
Derived from Fertilized Mouse Blastocyst. Stem Cells. 24: 2023-2033.
73.
Bui HT, Thuan NV, Wakayama T, and Miyano T. (2006) Chromatin remodeling in somatic cells
injected into mature pig oocytes Reproduction 131: 1037-1049.
74.
Yoneda A, Okada A, Wakayama T, Ueda J, and Watanabe T. (2006) Analysis of specific factors
generating 2-cell block in AKR mouse embryos. Zygote. 14:169-179.
75.
Kishigami S, Ohta H, Mizutani E, Wakayama S, Bui HT, Thuan NV, Hikichi T, Suetsugu R, andWakayama T.(2006) Harmful or Not: Trichostatin A treatment of embryos generated by ICSI or
ROSI. Central European Journal of Biology 1: 376-385.
76.
Wakisaka-Saito N, Kohda T, Inoue K, Ogonuki N, Miki H, Hikichi T, Mizutani E, Wakayama T,
Kaneko-Ishino T, Ogura A, and Ishino F. (2006) Chorioallantoic placenta defects in cloned mice.
Biochem Biophys Res Commun. 349: 106-114.
77.
Mizutani E, Ohta H, Kishigami S, Thuan NV, Hikichi T, Wakayama S, Kosaka M, Sato E, andWakayama T. Developmental ability of cloned embryos from neural stem cells. Reproduction.
2006 132:849-857.
78.

Singh U, Yu Y, Kalinina E, Konno T, Sun T, Ohta H, Wakayama T, Soares MJ, Hemberger M,
and Fundele RH. (2006) Carboxypeptidase E in the mouse placenta. Differentiation. 74:648-660.

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79.

Hikichi T, Wakayama S, Mizutani E, Takashima Y, Kishigami S, Van Thuan N, Ohta H, Bui
HT, Nishikawa SI, and Wakayama T. (2007) Differentiation Potential of Parthenogenetic

Embryonic Stem Cells is Improved by Nuclear Transfer. Stem Cells. 25:46-53.
80.
Kishigami S, Bui HT, Wakayama S, Tokunaga K, Thuan NV, Hikichi T, Mizutani E, Ohta H,
Suetsugu R, Sata T, and Wakayama T. (2007) Success of mouse cloning from an outbred strain by
TSA treatment after somatic nuclear transfer. J. Reprod. Dev. 53: 165-170.
81.
Wakayama S, Hikichi T, Suetsugu R, Sakaide Y, Bui HT, Mizutani E, and Wakayama T. (2007)
Efficient establishment of mouse embryonic stem cell lines from single blastomeres and polar
bodies. Stem Cells. 25:986-993.
82.
Bui HT, Van Thuan N, Kishigami S, Wakayama S, Hikichi T, Ohta H, Mizutani E, Yamaoka E,
Wakayama T, and Miyano T.(2007) Regulation of chromatin and chromosome morphology byhistone H3 modifications in pig oocytes. Reproduction. 133:371-382.
83.
Wakayama S, Suetsugu R, Thuan NV, Ohta H, Kishigami S, and Wakayama T. (2007)
Establishment of mouse embryonic stem cell lines from somatic cell nuclei by nuclear transfer into
aged, fertilization-failure mouse oocytes. Curr. Biol. 17: R120-R121
84.
Ohta H, Sakaide Y, and Wakayama T. (2007) Generation of progeny via ICSI following
enrichment of elongated spermatids from mouse testis by flow-cytometric cell sorting. Hum
Reprod. 22: 1612-1616
85.
Senda S, and Wakayama T, Arai Y, Yamazaki Y, Ohgane J, Tanaka S, Hattori N, Yanagimachi
R, and Shiota K. (2007) DNA Methylation Errors in Cloned Mice Disappear with Advancement ofAging, Cloning Stem Cells 9:293-302
86.
Kim K, Ng K, Rugg-Gunn PJ, Shieh JH, Kirak O, Jaenisch R, Wakayama T, Moore MA,
Pedersen RA, and Daley GQ. (2007) Recombination Signatures Distinguish Embryonic Stem Cells
Derived by Parthenogenesis and Somatic Cell Nuclear Transfer. Cell Stem Cells 1:346-352
87.
Kishigami S, and Wakayama T. (2007) Efficient Strontium-Induced Activation of Mouse
Oocytes in Standard Culture Media by Chelating Calcium. J. Reprod. Dev. 2007 53:1207-12157