EEAPVPF

TG/CASSAVAORIGINAL: English
DATE: 2013-07-03

EAST ASIA PLANT VARIETY PROTECTION FORUM

Cassava
Manihot esculenta (Crantz.)

GUIDELINESFOR THE CONDUCT OF TESTSFOR DISTINCTNESS, UNIFORMITY AND STABILITY

Alternative Names:
Botanical nameEnglishManihot esculenta Cassava(Crantz.)

Thai
Man sampalang

The purpose of these guidelines (“Test Guidelines”) is to fulfill the activities under the EAPVPForum on harmonization of Test Guidelines.

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TABLE OF CONTENTS

PAGE

1.

SUBJECT OF THESE TEST GUIDELINES........................................................................ 3

2.

MATERIAL REQUIRED...................................................................................................... 33.

METHOD OF EXAMINATION........................................................................................... 3
3.1
3.2
3.3
3.4
3.5

4.

Number of Growing Cycles......................................................................................... 3
Testing Place............................................................................................................... 3
Conditions for Conducting the Examination................................................................ 3
Test Design................................................................................................................... 3
Additional Tests............................................................................................................ 4

ASSESSMENT OF DISTINCTNESS, UNIFORMITY AND STABILITY........................ 4
4.1
4.2
4.3

Distinctness...................................................................................................................4
Uniformity.................................................................................................................... 5
Stability........................................................................................................................ 5

5.

GROUPING OF VARIETIES AND ORGANIZATION OF THE GROWINGTRIAL.................................................................................................................................... 5

6.

INTRODUCTION TO THE TABLE OF CHARACTERISTICS....................................... 6
6.1
6.2
6.3
6.4
6.5

Categories of Characteristics................................................................................. .......6
States of Expression and Corresponding Notes............................................................6
Types of Expression.................................................................................................... 7
Example Varieties........................................................................................................ 7
Legend.......................................................................................................................... 87.

TABLE OF CHARACTERISTICS…………................................……........…................... 9

8.

EXPLANATIONS ON THE TABLE OF CHARACTERISTICS.................................... 16
8.1
8.2

9.

Explanations covering several characteristics....................................................... .... 16
Explanations for individual characteristics................................................................ 16

LITERATURE.....................................................................................................................22

10. TECHNICAL QUESTIONNAIRE..................................................................................... 23

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1.

Subject of these Test Guidelines

These Test Guidelines apply to all varieties of Manihot esculenta Crantz.
In the case of ornamental varieties, it may, in particular, be necessary to use
additional characteristics to those included in the Table of Characteristics in order toexamine Distinctness, Uniformity and Stability.
2.

Material Required

2.1
The competent authorities decide on the quantity and quality of the plantmaterial required for testing the variety and when and where it is to be delivered. Applicants
submitting material from a State other than that in which the testing takes place mustensure that all customs formalities and phytosanitary requirements are complied with.
2.2

The material is to be supplied in the form of cuttings.

2.3

The minimum quantity of plant material, to be supplied by the applicant, should be:
At least 30 cuttings, each one with a length of at least 25 cm with at least 5 buds.

2.4
The plant material supplied should be visibly healthy, not lacking in vigor, noraffected by any important pest or disease.
2.5
The plant material should not have undergone any treatment which would affect theexpression of the characteristics of the variety, unless the competent authorities allow orrequest such treatment. If it has been treated, full details of the treatment must be given.
3.

Method of Examination

3.1

Number of Growing Cycles
The minimum duration of tests should normally be a single growing cycle. Further
growing cycle can be added if necessary.
3.2

Testing Place
Tests are normally conducted at one place. In the case of tests conducted at morethan one place, guidance is provided in TGP/9 “Examining Distinctness”.
3.3

Conditions for Conducting the Examination
The tests should be carried out under conditions ensuring satisfactory growth forthe expression of the relevant characteristics of the variety and for the conduct of theexamination.
3.4

Test Design

3.4.1 Each test should be designed to result in a total of at least 20 plants, spacing atleast 1 X 1 m, which should be divided between two or more replicates.
3.4.2 The design of the tests should be such that plants or parts of plants may be removed
for measurement or counting without prejudice to the observations which must be made up
to the end of the growing cycle.

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3.5

Additional Tests
Additional tests, for examining relevant characteristics, may be established.

4.

Assessment of Distinctness, Uniformity and Stability

4.1

Distinctness
4.1.1 General Recommendations

It is of particular importance for users of these Test Guidelines to consult theGeneral Introduction prior to making decisions regarding distinctness. However, thefollowing points are provided for elaboration or emphasis in these Test Guidelines.
4.1.2 Consistent Differences
The differences observed between varieties may be so clear that more than one
growing cycle is not necessary. In addition, in some circumstances, the influence of theenvironment is not such that more than a single growing cycle is required to provide
assurance that the differences observed between varieties are sufficiently consistent. One
means of ensuring that a difference in a characteristic, observed in a growing trial,
is sufficiently consistent is to examine the characteristic in at least two independent growing
cycles.
4.1.3 Clear Differences
Determining whether a difference between two varieties is clear depends on manyfactors, and should consider, in particular, the type of expression of the characteristic being
examined, i.e. whether it is expressed in a qualitative, quantitative, or pseudoqualitative manner. Therefore, it is important that users of these Test Guidelines arefamiliar with the recommendations contained in the General Introduction prior to making
decisions regarding distinctness.
4.1.4 Number of Plants / Parts of Plants to be examined
Unless otherwise indicated, for the purposes of distinctness, all observations on single
plants should be made on 10 plants or parts taken from each of 10 plants and any otherobservations made on all plants in the test, disregarding any off-type plants.
4.1.5 Method of Observation
The recommended method of observing the characteristic for the purposes of
distinctness is indicated by the following key in the second column of the Table ofCharacteristics (see document TGP/9 “Examining Distinctness”, Section 4 “Observationof characteristics”):
MG: single measurement of a group of plants or parts of plants
MS: measurement of a number of individual plants or parts of plants
VG: visual assessment by a single observation of a group of plants or parts of plants
VS: visual assessment by observation of individual plants or parts of plants

TG/Cassava
2013-07-03-5-Type of observation: visual (V) or measurement (M)“Visual” observation (V) is an observation made on the basis of the expert’s
judgment. For the purposes of this document, “visual” observation refers to thesensory observations of the experts and, therefore, also includes smell,taste and touch. Visual observation includes observations where the expert
uses reference points (e.g. diagrams, example varieties, side-by-side
comparison) or non-linear charts (e.g. color charts). Measurement (M) is anobjective observation against a calibrated, linear scale e.g. using a ruler,weighing scales, colorimeter, dates, counts, etc.
Type of record: for a group of plants (G) or for single, individual plants (S)For the purposes of distinctness, observations may be recorded as a single record
for a group of plants or parts of plants (G), or may be recorded as records for anumber of single, individual plants or parts of plants (S). In most cases, “G”
provides a single record per variety and it is not possible or necessary to apply
statistical methods in a plant-by-plant analysis for the assessment ofdistinctness.” In cases where more than one method of observing thecharacteristic is indicated in the Table of Characteristics (e.g. VG/MG),
guidance on selecting an appropriate method is provided in document TGP/9,
Section 4.2.
4.2

Uniformity

4.2.1 It is of particular importance for users of these Test Guidelines to consult theGeneral Introduction prior to making decisions regarding uniformity. However, thefollowing points are provided for elaboration or emphasis in these Test Guidelines:
4.2.2 For the assessment of uniformity of clones, a population standard of 1% and an
acceptance probability of at least 95% should be applied. In the case of a sample size of 20
plants, 1 off-type is allowed.
4.3

Stability

4.3.1 In practice, it is not usual to perform tests of stability that produce results as certain
as those of the testing of distinctness and uniformity. However, experience has
demonstrated that, for many types of variety, when a variety has been shown to be uniform,
it can also be considered to be stable.
4.3.2 Where appropriate, or in cases of doubt, stability may be tested, either by growing
a further generation, or by testing a new seed stock to ensure that it exhibits the samecharacteristics as those shown by the previous material supplied.
5.

Grouping of Varieties and Organization of the Growing Trial

5.1
The selection of varieties of common knowledge to be grown in the trial with thecandidate varieties and the way in which these varieties are divided into groups to facilitate
the assessment of distinctness are aided by the use of grouping characteristics.

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5.2
Grouping characteristics are those in which the documented states of expression,even where produced at different locations, can be used, either individually or in
combination with other such characteristics: (a) to select varieties of common knowledge
that can be excluded from the growing trial used for examination of distinctness; and (b)to organize the growing trial so that similar varieties are grouped together.
5.3

The following have been agreed as useful grouping characteristics:(a) Apical leaf: color (characteristic 13)(b) Apical leaf: pubescence (characteristic 14)(c) Petiole: color (characteristic 23)(d) Leaf: variegation (characteristic 16)(e) Petiole: attitude in relation to stem (characteristic 21)(f) Stem: color of exterior (characteristic 3)(g) Stem: growth habit (characteristic 5)(h) Root: external color (characteristic 27)

5.4
Guidance for the use of grouping characteristics, in the process of examining
distinctness, is provided through the General Introduction and document TGP/9 “Examining
Distinctness”.
6.

Introduction to the Table of Characteristics

6.1

Categories of Characteristics
6.1.1 Standard Test Guidelines Characteristics

Standard Test Guidelines characteristics are those which are approved by
UPOV for examination of DUS and from which members of the Union can select those
suitable for their particular circumstances.
6.1.2 Asterisked Characteristics
Asterisked characteristics (denoted by *) are those included in the Test Guidelines
which are important for the international harmonization of variety descriptions and should
always be examined for DUS and included in the variety description by all members of theUnion, except when the state of expression of a preceding characteristic or regional
environmental conditions render this inappropriate.
6.2

States of Expression and Corresponding Notes

6.2.1 States of expression are given for each characteristic to define the characteristic and toharmonize descriptions. Each state of expression is allocated a corresponding numericalnote for ease of recording of data and for the production and exchange of the description.
6.2.2 In the case of qualitative and pseudo-qualitative characteristics (see Chapter 6.3), allrelevant states of expression are presented in the characteristic. However, in the case ofquantitative characteristics with 5 or more states, an abbreviated scale may be used tominimize the size of the Table of Characteristics. For example, in the case of a quantitative
characteristic with 9 states, the presentation of states of expression in the Test Guidelines

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may be abbreviated as follows:
State

Note

small

3

medium

5

large

7

However, it should be noted that all of the following 9 states of expression exist to describe
varieties and should be used as appropriate:
State

Note

very small

1very small to small

2

small

3

small to medium

4

medium5

medium to large

6

large

7

large to very large

8very large

9

6.2.3 Further explanation of the presentation of states of expression and notes is provided
in document TGP/7 “Development of Test Guidelines”.
6.3

Types of Expression

An explanation of the types of expression of characteristics (qualitative, quantitativeand pseudo-qualitative) is provided in the General Introduction.
6.4

Example Varieties

Where appropriate, example varieties are provided to clarify the states of expressionof each characteristic.

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6.5

Legend(*)

Asterisked characteristic– see Chapter 6.1.2

QL:
QN:
PQ:

Qualitative characteristic
Quantitative characteristic
Pseudo-qualitative characteristic– see Chapter 6.3– see Chapter 6.3– see Chapter 6.3

MG, MS, VG, VS– see Chapter 4.1.5(a)-(b) See Explanations on the Table of Characteristics in Chapter 8.1(+)
See Explanations on the Table of Characteristics in Chapter 8.2

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7.

Table of Characteristics
Example variety
1
(*)
QN

Note

MS

Plant: height

(b)

short

3

medium5

VG

tall
Stem: color of cortex

7

2
PQ

(b)

light green

1dark green

2

cream

3

3
(*)
PQ

VG

purplish
Stem: color of exterior

RED cassava

4(b)orange1greyish yellow

2greenish yellow

3brownish yellow

4light brown

5

dark brown

6

VG

grey
Stem: color of epidermis(middle part of plant)

7

4

PQ

(b)

cream

1

light brown2

dark brown

3

orange4

purple5

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Example variety
5
(*)
(+)
QL

Note

VG

Stem: growth habit(b)straight

1
2

6

VG

zigzagStem: prominence of leaf scars(middle part of plant)

QN

(b)

weak

3

medium5
7

VG

strongYoung stem: main color(a)light green

MM122

1

yellowish green

MM183

2

MM181

3

7
(+)
PQ

8

VG

green
Young stem: presence of anthocyanin
coloration

QL

(a)

absent1
99VG

present
Young stem: Intensity of anthocyanin
coloration

QN

(a)

weak

3

medium5

VG

strongShoot tip: intensity of anthocyanin coloration

7

10
QN

(a)

weak

3

medium5strong7

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Example variety
11
(+)
QN

12
(+)
QN

13
(*)
(+)
PQ

Note

MG

Stem: number of branching level(b)absent

1

one

2

two

3

three

4

more than three
Stem: distance between leaf scars on nodes

5

MS
(b)

short

3

medium5

long
Apical leaf: color

7

VG

(a)

light green

Rayong 3

1

dark green

Rayong 90

2

purplish green
purple3Rayong 1

4

Hauybong 60

5

VG

light brown
Apical leaf: pubescence(a)absentRayong 91

Rayong 1

9

15

VG

present
Leaf: color

PQ

(a)

light green

1dark green

2

purplish green

3

purple

4

14
(*)
(+)
QL

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Example variety

Note

16
(*)
QL

VG

Leaf: variegation(a)absent1

MS

present
Leaf: length of central lobe

9

17
(*)
(+)
QN

(a)

short

3

medium5

long
Leaf: width of central lobe

7

MS

(a)

narrow3

medium

5
7

VG

broad
Leaf: predominant shape of central lobe(a)linear1

obovate2
3

VG

oblongLeaf: color of veins

(a)

green

Rayong 90

1

reddish green

Rayong 5

2

18
(*)
(+)
QN

19
(*)
(+)
PQ

20
(+)
PQ

21
(*)
(+)
PQ

red

3
4

VG

purplePetiole: attitude in relation to stem(a)semi erect1

horizontal

2drooping

3

irregular

4

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Example variety
22
(*)
QN

23
(*)
(+)
PQ

MS

Petiole: length

(a)

short

3

medium5

long
Petiole: color

7

VG

(a)

yellowish green

1light green

24
(*)
(+)
QN

Note

Rayong 90

2

green

3

reddish green

4

greenish red

5

red

6
7

VG

purpleStipule: length

(a)

short

3

medium5

VG

long
Stipule: margin

7

25
(*)
(+)
QL

(a)

entire

1

VG

split
Root : extent of neck

2

26
(*)
(+)
QL

(b)

absent1

present

9

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Example variety
27
(+)
PQ

Note

VG

Root: external color(b)whitish1

light brown2

medium brown3

dark brown

4

28

VG

reddish brown
Root: color of cortex

MM122

5

PQ

(b)

white

1

cream

2

yellow

3pink

4light brown

5

VG

purpleRoot: color of flesh

6

29
PQ

(b)

white

1

cream

2

yellow

3

VG

pink
Root: texture of epidermis

4

30
QL

(b)

smooth

1
2

VG

rough
Root: shape(b)conical1

conical to cylindrical2cylindrical3

irregular

4

31
(+)
PQ

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Example variety
32
(+)
QN

33
(+)
QN

34
(+)
QN

Note

MG

Root: cyanide content

(b)

low

3

medium5

high
Root: adherence of cortex to flesh

7

VG
(b)

weak

3

medium5
7

MG

strongRoot: content of starch in flesh

(b)

low

3

medium5

high

7

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8.

Explanations on the Table of Characteristics

8.1

Explanations covering several characteristics

Characteristics containing the following key in the second column of the Table ofCharacteristics should be examined as indicated below:(a) Observations should be made after 90-180 days (3-6 months) from planting as
leaf characteristics at the middle part of canopy as fully developed leaf(b) Observations should be made after 240 -360 days (8-12 months) from planting
8.2

Explanations for individual characteristics

Ad. 5: Stem: growth habit

1straight

2
zigzagAd. 7: Young stem: main color

MM122

1
light green

MM183

2
yellowish green

MM181

3
green

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Ad. 11: Stem: number of branching level2
one branching

4
three branching

Ad. 12: Stem: distance between leaf scars on nodes
The characteristic should be observed at the middle part of the plant, and two scars inthe same alignment are to be observed.
Ad. 13: Apical leaf: color

1
light green

2
dark green

3
purplish green

4
purpleAd. 14: Apical leaf: pubescence1absent9present

5
light brown

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Ad. 17: Leaf: length of central lobe

Ad. 18: Leaf: width of central lobe

Ad. 19: Leaf: predominant shape of central lobe

1linear2
obovate3
oblongAd. 20: Leaf: color of veins

1
green

2
reddish green

3redTG/Cassava
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Ad. 21: Petiole: attitude in relation to stem

1semi erect2
horizontal

3
drooping

4
irregular

Ad. 23: Petiole: color

12yellowish green light green

3
green

4
5
reddish green greenish red

6
red

7
purpleTG/Cassava
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Ad. 24: Stipule: length

3
short

5
medium7long

Ad. 25: Stipule: margin1
entire2
split

Ad. 26: Root: extent of neck1
absent9present

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Ad. 27: Root: external color

5
reddish brown

Ad. 31: Root: shape1
conical2
conical to cylindrical3cylindrical4
irregular

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Ad. 32: Root: cyanide content
Rapid screening assay of cyanide content of cassava (Williams and Edward (1980)
method) This is a rapid, inexpensive screening assay developed in order to measure thecyanide content of cassava (Manihot esculenta Crantz.) tubers.
A small disc ofparenchyma tissue cut with a cork borer or alternatively grated tissue placed in a stoppered
glass tube with a filter paper previously spotted with a drop of tetra-base [4,4'methylenebis-(N,N- dimethylaniline)] and cupric acetate and hydrogen. Cyanide liberated
produces a blue color on the filter paper. The intensity of the blue color developed within
one hour is rated visually on a graded scale from 0 to 5. The correlation coefficient
between samples accurately analyzed for total cyanide and also tested using the rapid assay
is 0.77.
Low cyanide content
0 to 1.9
Medium cyanide content
2.0 to 3.9
High cyanide content
4.0 to 5.0
Ad. 33 Root: adherence of cortex to flesh
Involves hand removal of root cortex from the middle third of freshly harvested root
tuber. Weak adherence is when the cortex is removed round the root tuber without any
breakage while strong adherence is when peeling of the cortex exhibits a lot of breaking andfor medium adherence there is minimal breaking of the cortex.
Ad. 34: Root: content of starch in flesh
Heritability for DM in cassava is relatively high; 0.87 broad sense heritability and0.51 – 0.67 narrow sense heritability (Kawano et al. 1987). Estimation of DM and starchcontent in cassava is based on the principle of a linear relationship between specific gravity
with DM and or starch content. Percentage DM = 158.3x – 142, while starch content =112.1x – 106.4;
where x = specific gravity. Specific gravity is measured according to thefollowing methodology:
1) Prepare root samples weighing 3–5 kg.
2) Weigh sample in air (Wa) using a suitable balance. Ensure that that the roots are
generally free from soil and other debris
3) Weigh the sample in water (Ww).
4) Ensure that you use the same container to weigh both in air and in water. A sturdy
wire basket works perfectly as it allows soil debris to fall through and also allows easymeasurement in water.
5) Compute specific gravity at Ww/(Wa – Ww)
6) Compute DM and starch content using the formulas
DM = 158.3x – 142, and starch content = 112.1x – 106.4.
9.

Literature

Fukuda, W.M.G., C.L. Guevara, R. Kawuki, and M.E. Ferguson. 2010. Selectedmorphological and agronomic descriptors for the characterization of cassava. International
Institute of Tropical Agriculture (IITA), Ibadan, Nigeria. 19 pp.
UPOV TG/cassav(proj.3)

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10.

Technical Questionnaire

TECHNICAL QUESTIONNAIRE

Page {x} of {y}Reference Number:

Application date:(not to be filled in by the applicant)
TECHNICAL QUESTIONNAIRE
to be completed in connection with an application for plant breeders’ rights

1.

2.

Subject of the Technical Questionnaire
1.1 Botanical Name

Manihot esculenta Crantz.

1.2 Common Name

Cassava

ApplicantName
Address

Telephone No.
Fax No.
E-mail address
Breeder (if different from applicant)3.

Proposed denomination and breeder’s reference
Proposed denomination(if available)
Breeder’s referenceTG/Cassava
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4.

Page {x} of {y}Reference Number:Information on the breeding scheme and propagation of the variety
4.1 Breeding scheme
Variety resulting from:
4.1.1 Crossing(a) controlled cross(please state parent varieties)(……………….)
female parent

X(…………………..)
male parent(b) partially know cross(please state know parent variety (ies))(……………….)
female parent
(c)

X

[ ]

[ ]

(…………………..)
male parent

unknow cross4.1.2 Mutation(please state parent variety)

[ ]
[ ]

4.1.3 Discovery and development[ ](please state where and when discovered and how developed)

4.1.4 Other(please provide details)[ ]TG/Cassava
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4.2

Page {x} of {y}Reference Number:

Method of propagating the variety
4.2.1 Vegetative propagation(a) cuttings[ ](b) in vitro propagation[ ](c) other (state method)[ ]4.2.2 Seed

[ ]4.2.3 Other(please provide details)[ ]TG/Cassava
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Page {x} of {y}Reference Number:

5.
Characteristics of the variety to be indicated (the number in brackets refers to thecorresponding characteristic in Test Guidelines; please mark the note which best corresponds).

5.1
(13)

Characteristics
Apical leaf: color

Example Varieties

Note

light green

Rayong 3

1[ ]dark green

Rayong 90

2[ ]purplish green
purpleRayong 1

4[ ]Hauybong 60

5[ ]absentRayong 91[ ]present

Rayong 1

9[ ]light brown
5.2
(14)

5.3
(19)

3[ ]

Apical leaf: pubescence

Leaf: predominant shape of central lobe

linear1[ ]obovate2[ ]oblong3[ ]TG/Cassava
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5.4
(23)

Page {x} of {y}Characteristics
Petiole: color

Reference Number:
Example Varieties

yellowish green
light green

5.5
(16)

5.6
(21)

5.7
(24)

Note

1[ ]
Rayong 90

2[ ]

green

3[ ]

reddish green

4[ ]greenish red

5[ ]

red

6[ ]

purple7[ ]Leaf: variegation

absent1[ ]present

9[ ]Petiole: attitude in relation to stemsemi erect1[ ]horizontal

2[ ]drooping

3[ ]irregular

4[ ]Stipule: length

short

3[ ]

medium

5[ ]

long

7[ ]

TG/Cassava
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5.8
(3)

5.9
(5)

Characteristics
Stem: color of exterior

Page {x} of {y}Reference Number:
Example Varieties

Noteorange1[ ]greyish yellow

2[ ]greenish yellow

3[ ]brownish yellow

4[ ]light brown5[ ]dark brown

6[ ]

grey

7[ ]

Stem: growth habit

straight

1[ ]zigzag2[ ]5.10 Root: external color(27)
whitish1[ ]light brown2[ ]medium brown3[ ]dark brown4[ ]reddish brown

MM122

5[ ]

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6.

Page {x} of {y}Reference Number:

Similar varieties and differences from these varieties

Please use the follow table and box for comments, to provide information on how your candidate
variety differs from the variety (or varieties) which, to the best of your knowledge, is (or are) mostsimilar. This information may help the examination authority to conduct its examination of
distinctness in a more efficient way.
Denomination(s) of
Characteristic(s) in Describe the expression Describe the expression
variety(ies) similar to which your candidate of the characteristic(s) of the characteristic(s)your candidate variety variety differs from thefor the similar
for your candidate
similar variety(ies)variety(ies)variety

Comments:

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Page {x} of {y}Reference Number:

7.

Additional information which may help in the examination of the variety

7.1

In addition to the information provided in sections 5 and 6, are there any additional
characteristics which may help to distinguish the variety?
Yes

[ ]

No

[ ]

(If yes, please provide details)

7.2

Special conditions for the examination of the variety
Are there any special conditions for growing the variety or conducting the examination?
Yes

[ ]

No

[ ]

(If yes, please give details)

7.3

Other information

8.

Authorization for release(a)

Does the variety require prior authorization for release under legislation concerning
the protection of the environment, human and animal health?
Yes

(b)

[ ]

No

[ ]

Has such authorization been obtained?
Yes

[ ]

No

[ ]

If the answer to (b) is yes, please attach a copy of the authorization.

TG/Cassava
2013-07-03- 31 -TECHNICAL QUESTIONNAIRE

9.

Page {x} of {y}Reference Number:Information on plant material to be examined or submitted for examination.

9.1 The expression of a characteristic or several characteristics of a variety may be affectedby factors, such as pests and disease, chemical treatment (e.g. growth retardants or pesticides),effects of tissue culture, different rootstocks, scions taken from different growth phases of atree, etc.
9.2 The plant material should not have undergone any treatment which would affect theexpression of the characteristics of the variety, unless the competent authorities allow or request
such treatment. If the plant material has undergone such treatment, full details of the treatment
must be given. In this respect, please indicate below, to the best of your knowledge, if the plantmaterial to be examined has been subjected to:(a)

Microorganisms (e.g. virus, bacteria, phytoplasma)

Yes [ ]No [ ](b) Chemical treatment (e.g. growth retardant or pesticide)

Yes [ ]No [ ](c)Tissue culture

Yes [ ]No [ ](d) Other factors

Yes [ ]No [ ]

Please provide details for where you have indicated “yes”.
……………………………………………………………

10.

I hereby declare that, to the best of my knowledge, the information provided in this form
is correct:
Applicant’s nameSignature

Date[End of document]