SEPTEMBER 2 ND - 6TH , 2024
Hamamatsu, JapanPROGRAM

Program
September 2nd (Monday)
14:30 - 16:30

Registration

Opening Session
16:30 - 17:30

Differences Between Plasmids and Chromosomes: Focus on DNA Compaction
and Physical Properties
Professor Hironori Niki (National Institute of Genetics, Japan)

18:00 – 19:30

Welcome Reception (Congress Center 3F – Room 31)

September 3rd (Tuesday)
Evolution (Plasmid-Mediated Bacterial Evolution)

Session Chair: Álvaro San Millán (CSIC, Spain)
Eva Top (University of Idaho, United States)
08:45 – 09:15

IL-1

Plasmids are drivers of adaptation in bacterial communities
Professor Michael Brockhurst (University of Manchester, United Kingdom)
IL-2

09:15 – 09:45

Short-term evolution of conjugative plasmids and consequences for
antimicrobial resistanceProfessor Tatiana Dimitriu (University of St Andrews, United Kingdom)

09:45 – 10:00

O1-1-1

Plasmids can promote microindel mutations
Mikkel Meyn Liljegren (UiT - The Arctic University of Norway, Norway)

10:00 – 10:15

O1-1-2The domestication of mobile DNA – a case study of virulence plasmidsof the genus Cronobacter
Rafal Jabluszewski (Institute of Microbiology, University of Warsaw, Poland)

10:15 – 10:30

Coffee Break

10:30 – 11:00

IL-3

Co-infection dynamics of mobile genetic elements
Professor Claudia Igler (University of Manchester, United Kingdom)
IL-4

11:00 – 11:30

TBD
Professor Lingchong You (Duke University, United States)

11:30 – 11:45

O1-2-1

Antibiotic resistance mutations promote bacterial evolvability throughan increase in plasmid recombination
Ignacio de Quinto Caceres (Hospital Ramón y Cajal (IRYCIS), Madrid, Spain)

11:45 – 12:00

O1-2-2

Unveiling plasmid functionality in a pristine groundwater systemdominated by candidate phyla radiation (CPR) bacteria
Olga Maria Perez Carrascal (Institute of Biodiversity, Friedrich Schiller University
Jena, Germany)International Symposium on Plasmid Biology 2024

September 3rd (Tuesday)
Evolution (Plasmid-Mediated Bacterial Evolution)
Session Chair: Álvaro San Millán (CSIC, Spain)
Eva Top (University of Idaho, United States)
O1-2-3

12:00 – 12:15

Beyond Borders: Unveiling the Impact of Horizontal Gene Transfer on
the Evolution of Plasmid Transfer Genes in Bacterial Communities
Olivia Kosterlitz (University of Idaho, United States)

12:30 – 13:30

Luncheon Seminar
Sponsored by: On-chip Biotechnologies Co., Ltd
Single-cell analysis of ‘plasmid holders’ in microbial communities
Professor Masaki Shintani (Shizuoka University, Japan)

Plasmid Classification and Databases (Bioinformatics)
Session Chair: Itzik Mirhazi (Ben-Gurion University, Israel)
Eva Top (University of Idaho, United States)

14:00 – 14:30

IL-5

Plasmid Taxonomy
Professor Fernando de la Cruz (CSIC, Spain)
IL-6

14:30 – 15:00

Uncovering and organizing the plasmid diversity across Earth’s
microbiomes
Professor Antonio Camrgo (Joint Genome Institute, Lawrence Berkeley National
Laboratory, United States)

15:00 – 15:15

O1-3-1

Establishing the Accuracy of a Plasmid Assembly Pipeline for ShortRead Sequence Contigs through PFGE Gel Mobility Size Verification
Caison Warner (University of California, Santa Cruz, United States)

15:15 – 15:30

O1-3-2

Plasmids: a number to rule them all
Paula Ramiro Martinez (Hospital Ramon y Cajal (IRYCIS), Madrid, Spain)

15:30 – 15:45

Coffee BreakIL-7

15:45 – 16:15

TBD
Professor Bruno Gonzalez-Zorn (Complutense University of Madrid, Spain)

16:15 – 16:30

O1-4-1

Unlocking new insights into plasmid mobility: Identification of the
conjugative oriTs
Manuel Ares Arroyo (Institut Pasteur, France)

16:30 – 16:45

O1-4-2Development of a sequence-based prediction method to determine
which bacteria receive plasmids by conjugation
Maho Tokuda (Shizuoka University, Japan)

16:45 – 17:00

O1-4-3

Tracking structural variation in carbapenem-resistance plasmids
carried by Enterobacteriaceae from bloodstream infections
Rob Moran (University of Birmingham, United Kingdom)

17:00 – 17:15

O1-4-4 Identifying plasmid outbreaks from short reads using RoundHound
Leah Roberts (EMBL-EBI, United Kingdom)International Symposium on Plasmid Biology 2024

September 3rd (Tuesday)
Plasmid maintenanceSession Chair: Barbara Funnell (University of Toronto)

17:30 – 18:00

IL-8

In vivo assembly of plasmid partition condensates: LLPS behavior,beyond trend and reality
Professor Jean-Yves Bouet (CNRS, France)
IL-9

18:00 – 18:30

Plasmid partition and host cell division control by ParA-family
ATPases with mechanisms involving reaction-diffusion principles
Professor Kiyoshi Mizuuchi (NIH, Japan)

18:30 – 18:45

O1-5-1

Single-cell RNA sequencing to understand bacterial heterogeneity andplasmid-host interactions
Rodrigo Ibarra Chavez (University of Copenhagen, Denmark )18:45 – 19:00

O1-5-2

An IncI2 plasmid partitioning system that co-regulates other plasmid
maintenance functions
Christopher Thomas (University of Birmingham, United Kingdom)

September 4th (Wednesday)
Ecology and Environmental Plasmid Biology

Session Chair: Søren J. Sørensen (University of Copenhagen, Denmark)Kornelia Smalla (Julius-Kuehn Institute, Germany)
IL-10

09:00 – 09:30

Intertwining plasmids, microbial interactions and adaptations to gut
environments
Professor Itzhak Mizrahi (Ben-Gurion University, Israel)

09:30 – 10:00

IL-11

Plasmid dynamics and maturation of the infant gut microbiome
Professor Søren J. Sørensen (University of Copenhagen, Denmark)

10:00 – 10:15

O2-1-1

What can we learn about marine plasmids from available microbiome
metagenomic data?
Shay Tal (Israel Oceanographic & Limnological Research, Israel)

10:15 – 10:30

O2-1-2

Assessing the role of bacterial innate and adaptive immunity as
barriers to conjugative plasmids
Berit Siedentop (Institute for Integrative Biology, ETH Zurich, Switzerland)

10:30 – 10:45

O2-1-3

Searching for conflicts between MGEs: novel fertility inhibition factors
against broad-host-range plasmids
Daniel Garcia Lopez (IBBTEC (CSIC-UC, Spain)

10:45 – 11:00

O2-1-4

How to lay low: decoding the regulation of a conjugative plasmidencoded T6SS
Maria del Mar Quinonero Coronel (IBBTEC (CSIC-UC), Spain)

11:00 – 11:15

Coffee BreakInternational Symposium on Plasmid Biology 2024

September 4th (Wednesday)
Other Mobile Genetic Elements and Computational Biology

Session Chair: Michael Brockhurst (University of Manchester, United Kingdom)O2-2-1

11:15 – 11:30

SGI1 encodes a fertility inhibitor of IncC plasmid and reveals
afunctional domain of MOBH1 relaxase
Florence Deschenes (Université de Sherbrooke, Canada)

11:30 – 11:45

O2-2-2

Plasmid or phage? Insights into the replicon of M13 gained from studyof staphylococcal plasmids of the pT181 family.
Chris Thomas (University of Leeds, United Kingdom)

11:45 – 12:00

O2-2-3

Unveiling plasmid functional elements using DNA language
Jianan Canal Li (University of California, United States)

Luncheon Seminar
Sponsored by: PacBio Japan
12:30 – 13:30

Antimicrobial Resistance and Plasmids in Microbiome
Professor Masato Suzuki(Antimicrobial Resistance Research Center, National
Institute of Infectious Diseases, Japan)

Spread of antibiotic resistance and pathogenicity

Session Chair: Kornelia Smalla (Julius-Kuehn Institute, Germany)
IL-12

14:00 – 14:30

Determinants of plasmid acquisition and selection
Professor Allison Lopatkin (University of Rochester, United States)

14:30 – 14:45

O2-3-1

Conjugative-killer plasmids, a novel antimicrobial alternative
Pedro Dorado Morales (Bacterial Genome Plasticity Unit- Institut Pasteur, France)

14:45 – 15:00

O2-3-2

Deciphering the molecular mechanisms of interaction between
conjugative plasmids and integrative mobilizable elements
Alfred Fillol Salom (Imperial College London, United Kingdom)

15:00 – 15:15

O2-3-3Composite-Sample Complex: Building a genomics model tounderstand the evolutionary history of antimicrobial resistance genemovement
Amy Mathers (University of Virginia, USA)

15:15 – 15:30

O2-3-4

Identification of plasmid-mediated antimicrobial resistance genes
among nordic biogas plants
Gabriela Kraychete (Federal University of Rio de Janeiro, Brazil)

15:30 – 16:00

Coffee Break

PosterSpread of antibiotic resistance and pathogenicity

16:00 – 18:00

Transcriptomic responses of clinical enterobacteria to carriage ofa carbapenem-resistance plasmid
Laura Toribio-Celestino (Centro Nacional de Biotecnologia, Spain)
P1-01

P2-02

Dissecting pOXA-48 fitness effects in clinical enterobacteria using
plasmid-wide CRISPRi screens
Alicia Calvo-Villamanan (National Centre for Biotechnology, CBB-CSIC, Spain)

International Symposium on Plasmid Biology 2024

September 4th (Wednesday)
PosterSpread of antibiotic resistance and pathogenicity

16:00 – 18:00

P1-03*Odd Numbers:

16:00 – 17:00

Even Numbers:

17:00 – 18:00

Studies aimed at improving the properties of conjugative pCURE
plasmids
Akram Sultan (University of Birmingham, United Kingdom)
P1-04

Hidden reservoirs of resistance: Contribution of plasmids to antibiotic
resistance in Staphylococcus aureus
Rachel Contarin (ANSES, France)
P1-05

Efficient transfer of a clinically derived IncP plasmid in murine gut
Melvin Yong (National University of Singapore, Singapre)
Dynamic changes in the plasmidome and resistome in thegastrointestinal tract of chickens
Marketa Rysava (University of Veterinary Sciences Brno, Czech Republic)
P1-06

P1-07

Assembly dynamics of the replicative transposition complex of
Tn4430 in vitro and in vivo
Loic Codemo(UCLouvain, Belgium)
Key mobile genetic carriers responsible for the dissemination ofmultidrug-resistant phenotypes among Enterobacter spp
Rafal Jabluszewski(University of Warsaw, Poland)
P1-08

Impact of transfer and resistance patterns on the spread of plasmidmediated resistance in Neisseria gonorrhoeae
Tabea Elsener (University of Oxford, United Kingdom)
P1-09

P1-10

Reporting carbapenemase-encoding plasmid transmission to publichealth labs
Nicole Lerminiaux (Public Health Agency of Canada, Canada)
P1-11

Identifying the Genetic Factors Involved in the Maintenance andSpread of the Conjugative Plasmid pOXA-48a
Yannick Baffert (Université Lyon, France)
P1-12

Unraveling the molecular mechanism of collateral sensitivity inducedby plasmid mediated beta-lactam resistance
Laura Alvaro Llorente (Ramon y Cajal Institute for Health Research, Spain)
Secondary functions of resistance genes: impact of ereA2 on
bacterial motility.
Alberto Hipolito (MBA lab. Universidad Complutense de Madrid, Spain)
P1-13

Genomic characterization of IncX3 plasmids harboring the blaNDM-5
carbapenemase gene isolated during Japanese national AMR surveillance in
2019-2020
Hui Zuo (AMR-Center, National Institute of Infectious Diseases, Japan)
P1-14International Symposium on Plasmid Biology 2024

September 4th (Wednesday)
PosterSpread of antibiotic resistance and pathogenicity

16:00 – 18:00

P1-15*Odd Numbers:

16:00 – 17:00

Even Numbers:

17:00 – 18:00

Polymicrobial Biofilms on Urinary Catheters
Philip Karlsson (Uppsala University,Sweden)
P1-16

Exploring plasmids carrying antimicrobial resistance genes in thehospital sink drain microbiome
Gregory McCallum (University of Liverpool, United Kingdom)
P1-17

PCA, PC-CVA and Random Forest of mass spectrometry data for the
elucidation of bacterial envelope differences and composition in antibiotic
resistance research
Alfred Fransson (University of Gothenburg, Sweden)
P1-18

The Impact of Pesticides on the Transmission of Antibiotic Resistance
Silvana Smits (University of Gothenburg, Gothenburg, Sweden)
P1-19

Characterization of the vanA-plasmid in the vancomycin-resistantEnterococcus faecium ST80 outbreak in Hiroshima, Japan
Takaya Segawa (National Institute of Infectious Diseases, Japan)
P1-20

Pervasive crosstalk between plasmid and chromosome unveils
therapeutic targets
Cristina Herencias (Instituto Ramón y Cajal de Investigación Sanitaria, Spain)
P1-21

Trimethoprim-resistant Bacteria and Genes: A Case Study of an Urban
River in India
Madhu Kumar Kumara (Indian Institute of Technology Hyderabad, India)
P1-22

Understanding antibiotic-induced blooms driving horizontal genetransfer of antibiotic resistance
Laura de Nies (University of Oxford, United Kingdom)
P1-23

Dissemination of Ceftriaxone-Resistant Salmonella Enteritidis
harboring plasmids encoding blaCTX-M-55 or blaCTX-M-14 gene in China
Xiaoting Hua (Zhejiang University, China)
P1-24
DNA binding mechanism of TraA relaxase from gram-positive Type IV
secretion system
Kirill Kuhlmann (University of Graz, Austria)

Unravelling the molecular mechanisms of Type IV-A3 CRISPR-Cas andits ecological significance in plasmid competition
Fabienne Benz (Institut Pasteur, France)
P1-25

Multilevel transmission of ESBL genes driven by plasmids and othermobile genetic elements across One Health network
Dr Timmer Balint (University of Debrecen, Hungary)P1-27 Interplay between Temperature and Carriage of Multiple Plasmids
Influences KPC PrevalenceKatie Barry (University of Virginia, United States)
P1-26

International Symposium on Plasmid Biology 2024

September 4th (Wednesday)
PosterSpread of antibiotic resistance and pathogenicity

16:00 – 18:00

P1-28*Odd Numbers:

16:00 – 17:00

Even Numbers:

17:00 – 18:00

Detecting AMR plasmid variants in Shigella sonnei
Sally Partridge (The Westmead Institute for Medical Research, Australia)
P1-29Enterococcal pELF1-type linear plasmids spread antimicrobial
resistance genes across genera in the environment
Yusuke Hashimoto (Gunma University Graduate School of Medicine, Japan)
P1-30

Identification of transfer Pseudomonas plasmids carrying carbapenem
resistance genes in the Philippines
Rin Yamazaki (Shizuoka University, Japan)
P1-31

Emergence of Acinetobacter towneri harboring a novel plasmid
withblaNDM-1 and tet(X7) from a hospital wastewater in the Philippines
Zoe Mallonga (University of the Philippines Tacloban College, Phillipines)
P1-32

Comparative sequence analysis of IncP-1 related tetracycline resistant
plasmids in N. gonorrhoeae
Ryota Ishii (Keio University, Japan)
P1-33

Mosaic mobile elements provide insight in the rapid acquisition ofmultiple antimicrobial resistances in Staphylococcus pseudintermedius
Linda van der Graaf (Utrecht University, the Netherlands, Netherlands)
P1-34

Engineering phagemids to tackle plasmid-mediated antibiotic
resistance
Ada Munoz Cazalla (Microbiology service, IRYCIS, Spain)
P1-35

Molecular mechanism of the TraI relaxase dimer loading onto oriT
Danylo Gorenkin (University of London, United Kingdom)
P1-36

Phenogenetic dynamics of blaNDM-1 gene adaptive amplification
Mario Pulido Vadillo (Complutense University of Madrid, Spain)
P1-37

Transmission of Antibiotic Resistance among Enterobacteriaceae
across Habitats
Xiaoqing Xu (The University of Hong Kong, Hong Kong)
P1-38

The expression of integron arrays is shaped by the translation rate ofcassettes
Jose Antonio Escudero (University Complutense of Madrid, Spain)

Plasmid maintenance
P1-39

Method for efficient quantification of short-term plasmid loss kinetics
Christina Egami (UC Santa Cruz, United States)
P1-40

Interbacterial killing by a conjugative plasmid in Vibrio cholerae
Celine Fetz (EPFL, Switzerland)International Symposium on Plasmid Biology 2024

September 4th (Wednesday)
PosterPlasmid maintenance

A good defense is a bad offense: CRISPR-Cas in inter-plasmid
competition*Odd Numbers: David Sunderhauf (University of Exeter, United Kingdom)
16:00 – 18:00
16:00 – 17:00

Even Numbers:

17:00 – 18:00

P41

P1-42

Killing of low- and non-receptive recipients by a multidrug-resistantconjugative plasmid
Yahua Chen (National University of Singapore, Singapore)
P1-43

Immediate Transcriptional Response of Host Cells Triggered by
Plasmid Acquisition
Chihiro Liu (The University of Tokyo, Japan)
Molecular investigation of the role of ParB on the segregation ofvirulence plasmid pB171 in Escherichia coli
Asmaa Alhelal (University of York, United Kingdom)
P1-44

P1-45

Host metabolism reducing fitness costs loaded by plasmid carriage
Masaaki Hidaka (The University of Tokyo, Japan)

Plasmid classification and databases (bioinformatics)
P1-46

Classifying the wild diversity of Rhizobium repABC plasmids
Peter Young (University of York, United Kingdom)
DFAST_QC: Rapid Quality Checking and Taxonomic Identification Tool
for Prokaryotic Genomes
Mohamed Elmanzalawi (The Graduate University for Advanced Studies (SOKENDAI),Japan)
P1-47

P1-48

Annotating plasmid genomes using DFAST
Yasuhiro Tanizawa (National Institute of Genetics, Japan)
P1-49

Suitability of plasmids as biological safety measures according to theGerman Genetic Engineering law
Andre Friedrich (Federal Office of Consumer Protection and Food Safety, Germany)
P1-50

Multiple variants of IncF-type alleles challenge plasmid typing
Michaela Ruzickova (CEITEC, VETUNI, Czech Republic)
P1-51

Novel in silico approaches for identifying previously unclassified
plasmids in Pseudomonas species based on replicon types
Rani Rudramadevi Sreerama (Shizuoka University, Japan)
P1-52

Genotypic reassessment of Pseudomonas plasmid incompatibility
groups according to recent sequence-based approaches
Yosuke Nishimura (JAMSTEC, Japan)

International Symposium on Plasmid Biology 2024

September 5th (Thursday)
Plasmid Replication

Session Chair: Christopher Thomas (University of Birmingham, United
Kingdom)

08:45 – 09:15
09:15 – 09:30

09:30 – 09:45

09:45 – 10:00

IL-13

Initiators temporal occupation of replication origins
Professor Igor Konieczny (University of Gdańsk, Poland)O3-1-1

Molecular switching of a DNA-sliding clamp to a repressor mediates
long-range gene silencing on a multi-drug resistant plasmid
Thomas McLean (John Innes Centre, United Kingdom )O3-1-2

Secondary chromosome of Allorhizobium vitis S4 contains a novel
structural type of repABC replication-partitioning modules
Elvira Krakowska (University of Warsaw, Warsaw, Poland)O3-1-3

Dissection of DNA transactions during conjugative plasmid transfer
Yoshiharu Yamaichi (I2BC / CNRS, Japan)
O3-1-410:00 – 10:15

Plasmid copy number variation in bacterial pathogenesis andantibiotic resistanceHelen Wang (Uppsala University, Sweden)

10:15 – 10:30

Coffee BreakSpread of Antibiotic Resistance and Pathogenicity

Session Chair: Kornelia Smalla (Julius-Kuehn Institute, Germany)
IL-14

10:30 – 11:00

The transferable resistome of biosolid bacteria influenced by the sizeof the wastewater treatment plant?
Professor Kornelia Smalla (Julius-Kuehn Institute, Germany)

11:00 – 11:15

O3-2-1

Plasmid-encoded insertion sequences promote rapid adaptation ofclinical enterobacteria
Jorge Sastre-Dominguez (Centro Nacional de Biotecnologia (CNB-CSIC), Madrid,
Spain)

11:15 – 11:30

O3-2-2

The Line of Duty: How to maintain a giant linear plasmid in antibioticproducing Streptomyces
Leah McPhillips (John Innes Centre, United Kingdom)

11:30 – 11:45

O3-2-3

Plasmid copy number increase mediates piperacillin-tazobactam
heteroresistance in Escherichia coli
Elin Svedholm (Uppsala University, Sweden)

11:45 – 12:00

O3-2-4

Megaplasmid-mediated spreading of the RND-type multidrug efflux
pump gene cluster tmexCD-toprJ among Pseudomonas species
Aki Hirabayashi (National Institute of Infectious Diseases, Japan)

12:00 – 13:30

Lunch BreakInternational Symposium on Plasmid Biology 2024

September 5th (Thursday)
Spread of Antibiotic Resistance and Pathogenicity

Session Chair: Kornelia Smalla (Julius-Kuehn Institute, Germany)

13:30 – 14:00

14:00 – 14:15
14:15 – 14:30

14:30 – 14:45

14:45 – 15:00

IL-15

Mating Pair Formation Complex of a Type IV Secretion System from
Gram-positive Pathogens: Mechanistic and Structural Insights
Professor Elisabeth Grohmann (Berlin University of Applied Sciences, Germany)O3-3-1

Virulence plasmids of extra-intestinal pathogenic Escherichia coli
Zheng Jie Lian (The University of Queensland,Australia)
O3-3-2Chromosomal insertion of blaCTX-M genes in Escherichia coli inAustralia
Alicia Fajardo Lubian (The University of Sydney, Australia)
O3-3-3 Genomics studies support the role of food strains of E. coli as plasmid
reservoirs for genetic exchange of virulence and antibiotic resistance genes withclinical strains
Manel Camps (University of California Santa Cruz, United States)O3-3-4

Convergent evolution of Enterobacteriaceae in epidemiologicalnetworks with high antimicrobial use
Yi Ling Tam (University of Bath, United Kingdom)
O3-3-515:00 – 15:15

Multidrug resistance plasmids commonly reprogramme expressionof metabolic genes in Escherichia coli
Rebecca Hall (University of Birmingham, United Kingdom)

15:15 – 15:30

Coffee Break

PosterPlasmid transfer

CAAX protease and bacteriocin-processing (CPBP)-type*Odd Numbers: intramembrane proteases mediate competitive transfer between conjugative
15:30 – 16:30
plasmids
Even Numbers:
Sarah Bigot (MMSB-CNRS-University Lyon-France, France)
15:30 – 17:30

16:30 – 17:30

P2-01

P2-02

pMG1-like eneterococcal highly conjugative plasmids
Haruyoshi Tomita (Gunma University Graduate School of Medicine, Japan)
Restriction modification systems play a major role in carbapenemaseencoding plasmid acquisition in hvKp
Guodong Oo (Yong Loo Lin School of Medicine ,Singapore)
P2-03

P2-04
Assessment of Bacillus subtilis plasmid pLS20 conjugation in theabsence of quorum sensing repression
Kenichi Yoshida (Kobe University, Japan)

Identification of unique conjugation genes in pELF1-type enterococcal
linear plasmid family
Jun Kurushima (Gunma University, Japan)
Deciphering the structure and function of the transcriptional
P2-06
terminator PrfaH for understanding the conjugation regulation of IncX Plasmids
Jun Yang (Guangdong Pharmaceutical University, China)
A genomics to genetics approach to uncovering entry exclusion
P2-07
interactions during bacterial conjugation
Wen Wen Low (National University of Singapore, Singapore)
P2-05

International Symposium on Plasmid Biology 2024

September 5th (Thursday)
PosterPlasmid transfer

15:30 – 17:30

P2-08*Odd Numbers:
15:30 – 16:30
Even Numbers:
16:30 – 17:30

In vitro transferability of ESBL-harboring IncI1 plasmids between
chicken and human isolates of Escherichia coli.
Matteo Buffoni (UMC Utrecht, department of Medical Microbiology ,The Netherlands)
P2-09

Nucleoside analogues as conjugation inhibitors in Escherichia coli andKlebsiella pneumoniae
Ilyas Alav (University of Birmingham ,United Kingdom)
P2-10 Roles of Phospholipids in Conjugative Transfer
Kouhei Kishida (Tohoku University ,Japan)
P2-11

Distinct clustering of antibiotic resistance genes in Escherichia coli
reveals contributions of genetic linkage and selection to shaping the evolutionof antibiotic resistanceManel Camps (University of California, United States)
P2-12

Analysis of factors affecting the efficiency of plasmid transfer in
Bacillus subtilis natto.
Wakana Suda (Tokyo University of Agriculture, Japan)
Characterization of features in replication and conjugative transfer ofplasmids in the novel subgroups of IncP/P-1
Shunta Tsuruga (Shizuoka University, Japan)
P2-13

P2-14

Hierarchical organization of complementary strand synthesis during
conjugative plasmid establishment
Nathan Fraikin (Molecular Microbiology and Structural Biochemistry, France)
Deciphering the role of proteins of the broad-host-range Firmicutes
T4SS from the conjugative plasmid pIP501
Claudia Michaelis (Berliner Hochschule für Technik ,Germany)
P2-15

P2-16

Campylobacter plasmid typing and transfer between species
Linda van der Graaf (Utrecht University, the Netherlands ,Netherlands)
In vivo assembly of bacterial partition condensates: LLPS behavior,
P2-17
beyond trend and reality
Hirotada Mori (Lab of Syst & Syn Microbiol, Guandong Academy of Agr. Sci, China)
P2-18
Plasmids Fight Back: Anti-Defense Systems Boost Conjugation
Efficiency
Bruria Samuel (Tel-Aviv University, Israel)

Evolution (plasmid-mediated bacterial evolution)
How do interactions between mobile genetic elements enhanceP2-19
resistance gene spread?
Victoria Orr (University of Liverpool ,United Kingdom)
P2-20

The Plasmidome of Extra-Intestinal Pathogenic E. coli Lineages
Joao A Gama (Ramón y Cajal Institute for Health Research, Madrid, Spain)
Temporal GWAS identifies a widely distributed putative adhesin
carried on a medium-copy number plasmid contributing to pathogen success in
Shigella spp.
P Malaka De Silva (University of Cambridge ,United Kingdom)
P2-21International Symposium on Plasmid Biology 2024

September 5th (Thursday)
Poster

Evolution (plasmid-mediated bacterial evolution)

Where compensatory mutations for plasmid costs occur is determined*Odd Numbers: by cost reduction and its trade-offs with resistance and conjugation
15:30 – 16:30
Christopher Witzany (ETH Zurich ,Switzerland)
Even Numbers:
P2-23 Investigating Selection for Horizontal and Vertical Transfer in IncP
16:30 – 17:30
Plasmids
Elizabeth Duan (University of Washington, Department of Biology, United States )15:30 – 17:30

P2-22

P2-24

Plasmid and Prejudice: A tale of plasmid-host compatibility in
Pseudomonas aeruginosa
Prajwal Vishwanath Bharadwaj (University of Liverpool, United Kingdom)
P2-25 A new approach to study the evolution of bacterial plasmids
Kamil Krakowski (University of Warsaw, Poland)
P2-26

Mobile genetic elements carrying the genes for γhexachlorocyclohexane degradation in sphingomonads
Yuji Nagata (Tohoku University, Japan)
P2-27

Inferring gains and losses of the 2,4-dichlorophenol hydroxylase gene(tfd) in IncP-1 plasmids from phylogenetic trees and gene presence/absence
patterns
Tianyu Lu (Keio University, Japan)
P2-28 Revisiting the taxonomic distribution and phylogenetic relationships ofthe membrane-associated guanylate kinase (MAGUK) family of scaffolding
proteins
Lianne Cagalingan (Keio University, Japan)
P2-29

A continuously evolving DNA barcode for plasmid lineage tracing
Sophia Wiesenfeld (Harvard Medical School, United States)

Ecology and environmental plasmid biology
P2-30

Long reads reveal complete plasmids in the genus Tenacibaculum
Ashton Sies (The University of Regina ,Canada)
P2-31

Sigma factor BacL2 promotes pheromone-induced expression ofbacteriolysin Bac41 to enhance HGT in Enterococcus faecalis
Jun Kurushima (Gunma University, Japan)
P2-32

Single-cell analysis identifies the original hosts of PromA plasmid
Megumi Masumoto (Shizuoka University, Japan)
P2-33

The key metabolic genes for alkane degradation in Rhodococcus
qingshengii N9T-4 were located in their two plasmids, pN9T4-1 and pN9T4-2.
Yuka Sato (Shizuoka University, Japan)
P2-34

Comprehensive plasmidome characterization of a model cow rumen
microbiome system
Rebecca Garner (University of California, Berkeley, United States)
P2-35
Ecological Dynamics of Marine Plasmids: Insights from Global
Metagenomic Analysis
Lucy Androsiuk (Ben-Gurion University of the Negev, Israel)

International Symposium on Plasmid Biology 2024

September 5th (Thursday)
PosterEcology and environmental plasmid biology

Worldwide diversity of genotypes determining the bacterial catabolism*Odd Numbers: of 2,4-D in 2,4-D treated arable fields
15:30 – 16:30
Anahita Modabberi (KU Leuven,Belgium)
Even Numbers:
P2-37 Using Hi-C and target capture to monitor plasmid transfer in the barley
16:30 – 17:30
rhizosphere
Thibault Stalder (Inserm ,France )15:30 – 17:30

P2-36

P2-38

Genomic epidemiology of Acinetobacter baumannii plasmids fromenvironmental and clinical sources
Soon Keong Wee (Nanyang Technological University, Singapore )Other mobile genetic elements and Computational biology
P2-39Interplay between the Xer system and the dissemination of
antibioresistance in Acinetobacter baumannii
Corentin Blanchais (LMGM-CBI, France)
P2-40

VicMAG: visualizing circular metagenome-assembled genomes
focused on bacterial virulence and antimicrobial resistance
Yusuke Tsuda (Kyoto University Hospital, Japan)
P2-41

Identification of the cellular interactants of the bacterial transposon

Tn4430Claire Stulemeijer (UCLouvain, Belgium)

P2-42 Variation in the plasmid backbone and dif module content of R3-T33
Acinetobacter plasmids
Stephanie Ambrose (The University of Sydney, Australia)
P2-43

Systematic analysis of prophages, other types of integrative elements
and defense systems in the Serratia marcescens complex using 142 closed
genomes
Debora S Nagano (Kyushu University, Japan)
P2-44

Vibrio parahaemolyticus encode diverse type I-F CRISPR-Cas variants
in mobile genetic elements
Carlos Emmanuel Panerio (University of the Philippines Diliman ,Phillipines)
P2-45

Engineering staphylococcal prophages for therapeutic applications
against methicillin-resistant Staphylococcus aureus
Huong Minh Nguyen (Jichi Medical University ,Japan)
P2-46

Leveraging protein language models to study hyperparasitic mobile
genetic elements
Urvish Trivedi (University of Copenhagen, Denmark)P2-47

Unraveling the role of plasmids in disseminating prokaryotic immune
systems across different ecosystems
Mario Rodriguez Mestre (University of Copenhagen, Denmark)Prevalence of the insertion sequence IS1071 in differentiallyanthropogenically impacted soils and its contribution to microbial community
adaptation
Dirk Springael (KU Leuven, Belgium)
P2-48

International Symposium on Plasmid Biology 2024

September 5th (Thursday)
PosterOther mobile genetic elements and Computational biology

15:30 – 17:30

P2-49*Odd Numbers:
15:30 – 16:30
Even Numbers:
16:30 – 17:30

Monitoring gene recruitment and lateral gene transfer of adaptive
genes by the bacterial insertion sequence IS1071 in complex microbialcommunities
Mah-e-Neema Nawaz (KU Leuven, Belgium)

Plasmid replication
P2-50

Discovery of Novel Replication Proteins for Large Plasmids in
Cyanobacteria and Their Potential Applications in Genetic Engineering.
Satoru Watanabe (Tokyo University of Agriculture, Japan)
P2-51

Studies on novel plasmid replication factors found in cyanobacteria
and their replication initiation mechanisms.
Minori Sakata (Tokyo University of Agriculture, Japan)
P2-52

Prediction and comparison of repA promoters in different PromA

plasmids
Nanako Isogai (Shizuoka University, Japan)
P2-53 The unique structure and properties of the replication system
of Klebsiella pneumoniae plasmid pIGMS31
Pawel Wawrzyniak (University of Warsaw)

September 6th (Friday)
Plasmid Transfer

Session Chair: Fernando de la (CSIC, Spain)Álvaro San Millán (CSIC, Spain)
IL-16

09:00 – 09:30

Plasmid CRISPR Conflicts: Type IV-A3 in the Spotlight
Professor Rafael Pinilla Redondo (University of Copenhagen, Denmark)

09:30 – 10:00

IL-17

The architecture and function of an antimicrobial resistancepropagator
Professor Tiago R D Costa (Imperial College London, United Kingdom)

10:00 – 10:15

A functional genomic approach to unravel complex genetic
mechanisms of AMR plasmid stability and transfer
Minh Duy Phan (The University of Queensland, Australia)

10:15 – 10:30

Coffee Break

10:30 – 11:00
11:00 – 11:30
11:15 – 11:30

11:30 – 11:45

O4-1-1

IL-18

DNA transfer by conjugation in live cells
Professor Christian Lesterlin (CNRS, France)
ML-01
Plasmids & Pill: an appreciation of Laura Frost(1949-2023)
Professor Beth A. Traxler (University of Washington, United States)
O4-2-1 Study of exclusion systems protecting against the acquisition of the Fplasmid by horizontal transfer
Couturier Agathe (MMSB CNRS, France)
O4-2-2

Interruption of the tir genes in IncL and IncM plasmids does not leadto higher conjugation frequencies but rather growth inhibition
Muhammad Kamruzzaman(Westmead Institute for Medical Research, Australia)

International Symposium on Plasmid Biology 2024

September 6th (Friday)
12:00 – 13:30

Lunch Break

Plasmid Transfer

Session Chair: Fernando de la (CSIC, Spain)Álvaro San Millán (CSIC, Spain)

13:30 – 13:45

The recipient cell modulates donor cell activation and conjugation
through the expression of capsule
Galain C Williams (Monash University, Australia)

13:45 – 14:00

O4-3-2

Should I stay or should I go:
Optimal timing of prophage induction and plasmid conjugation
Jana Sanne Huisman (Massachusetts Institute of Technology, United States)

14:00 – 14:15

Exploring the potential of bacterial conjugation in gene editing: A
delivery mechanism for relaxase-driven CRISPR-Cas system
Dolores Guzman Herrador (IBBTEC-UC, Spain)

14:15 – 15:00

Coffee Break

15:00 – 16:30

ISPB meeting, Special LectureO4-3-1O4-3-3

Professor Hideaki Nojiri (The University of Tokyo)

18:00 – 20:00

Banquet (Okura ACT CITY HOTEL 4F "Heian")International Symposium on Plasmid Biology 2024