研究業績

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著者名
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所属

日本におけるヒト T 細胞白血病ウイルス 1 型定量 PCR の標準検査法の開発
Kuramitsu M， Okuma K， Nakashima M， Sato T， Sasaki D， Hasegawa
H， Umeki K， Kubota R， Sasada K， Sobata R， Matsumoto C， Kaneko
N， Tezuka K， Matsuoka S， Utsunomiya A， Koh KR， Ogata M， Ishitsuka
K， Taki M， Nosaka K， Uchimaru K， Iwanaga M， Sagara Y， Yamano
Y， Okayama A， Miura K， Satake M， Saito S， Watanabe T， HamaguchiI．
1Department of Safety Research on Blood and Biological Products, National
Institute of Infectious Diseases, Tokyo, Japan.
2
Department of Computational Biology and Medical Sciences, Graduate
School of Frontier Sciences, The University of Tokyo, Tokyo, Japan.
3
Department of Rare Diseases Research, Institute of Medical Science, St.
Marianna University School of Medicine, Kawasaki, Japan.
4
Department of Laboratory Medicine, Nagasaki University Hospital, Nagasaki,Japan.
5
Department of Rheumatology, Infectious Diseases and Laboratory Medicine,
University of Miyazaki, Miyazaki, Japan.
6
Division of Molecular Pathology, Center for Chronic Viral Diseases,Kagoshima University, Kagoshima, Japan.
7
Department of Laboratory Medicine, Kumamoto University Hospital,Kumamoto, Japan.
8Central Blood Institute, Blood Service Headquarters, Japanese Red Cross
Society, Tokyo, Japan.
9Department of Infection and Immunology, SRL Inc., Tokyo, Japan.
10
Department of Hematology, Imamura General Hospital, Kagoshima, Japan.
11
Department of Hematology, Osaka General Hospital of West Japan Railway
Company, Osaka, Japan.
12Department of Hematology, Oita University Hospital, Oita, Japan.
13
Division of Hematology and Immunology, Center for Chronic Viral
Diseases, Kagoshima University, Kagoshima, Japan.
14
Rakuwakai Kyoto Medical Examination Center, Kyoto, Japan.
15
Department of Hematology, Kumamoto University of Medicine, Kumamoto,Japan.
16
Department of Hematology and Oncology, Research Hospital, Institute ofMedical Science, The University of Tokyo, Tokyo, Japan.
17
Department of Frontier Life Science, Nagasaki University Graduate Schoolof Biomedical Sciences, Nagasaki, Japan.
18
Japanese Red Cross Kyushu Block Blood Center, Fukuoka, Japan.
19
Department of Obstetrics and Gynecology, Nagasaki University Graduate
School of Biomedical Sciences, Nagasaki, Japan.
20
Department of Obstetrics and Gynecology, University of Toyama, Toyama,
Japan.
21
Department of Advanced Medical Innovation, Graduate School of Medicine,
St. Marianna University, Kawasaki, Japan.
英文TitleAuthor
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Affiliation 
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Development of reference material with assigned value for human T-cellleukemia virus type 1 quantitative PCR in Japan．
Kuramitsu M， Okuma K， Nakashima M， Sato T， Sasaki D， Hasegawa
H， Umeki K， Kubota R， Sasada K， Sobata R， Matsumoto C， Kaneko
N， Tezuka K， Matsuoka S， Utsunomiya A， Koh KR， Ogata M， Ishitsuka
K， Taki M， Nosaka K， Uchimaru K， Iwanaga M， Sagara Y， Yamano
Y， Okayama A， Miura K， Satake M， Saito S， Watanabe T， Hamaguchi
I．
1
Department of Safety Research on Blood and Biological Products, National
Institute of Infectious Diseases, Tokyo, Japan.
2
Department of Computational Biology and Medical Sciences, Graduate
School of Frontier Sciences, The University of Tokyo, Tokyo, Japan.
3
Department of Rare Diseases Research, Institute of Medical Science, St.
Marianna University School of Medicine, Kawasaki, Japan.
4
Department of Laboratory Medicine, Nagasaki University Hospital, Nagasaki,Japan.
5
Department of Rheumatology, Infectious Diseases and Laboratory Medicine,
University of Miyazaki, Miyazaki, Japan.
6
Division of Molecular Pathology, Center for Chronic Viral Diseases,Kagoshima University, Kagoshima, Japan.
7
Department of Laboratory Medicine, Kumamoto University Hospital,Kumamoto, Japan.
8Central Blood Institute, Blood Service Headquarters, Japanese Red Cross
Society, Tokyo, Japan.
9Department of Infection and Immunology, SRL Inc., Tokyo, Japan.
10
Department of Hematology, Imamura General Hospital, Kagoshima, Japan.
11
Department of Hematology, Osaka General Hospital of West Japan Railway
Company, Osaka, Japan.
12Department of Hematology, Oita University Hospital, Oita, Japan.
13
Division of Hematology and Immunology, Center for Chronic Viral
Diseases, Kagoshima University, Kagoshima, Japan.
14Rakuwakai Kyoto Medical Examination Center, Kyoto, Japan.
15
Department of Hematology, Kumamoto University of Medicine, Kumamoto,Japan.
16
Department of Hematology and Oncology, Research Hospital, Institute ofMedical Science, The University of Tokyo, Tokyo, Japan.
17
Department of Frontier Life Science, Nagasaki University Graduate Schoolof Biomedical Sciences, Nagasaki, Japan.
18
Japanese Red Cross Kyushu Block Blood Center, Fukuoka, Japan.
19
Department of Obstetrics and Gynecology, Nagasaki University Graduate
School of Biomedical Sciences, Nagasaki, Japan.
20
Department of Obstetrics and Gynecology, University of Toyama, Toyama,Japan.
21
Department of Advanced Medical Innovation, Graduate School of Medicine,
St. Marianna University, Kawasaki, Japan.

Abstract

Quantitative PCR (qPCR) of human T-cell leukemia virus type 1 (HTLV-1)
provirus is used for HTLV-1 testing and for assessment of risk of HTLV-1related diseases. In this study, a reference material was developed forstandardizing HTLV-1 qPCR. Freeze-dried TL-Om1 cells diluted with Jurkat
cells were prepared and an assigned value for proviral load (PVL) of 2.71
copies/100 cells was determined by digital PCR. Nine Japanese laboratories
using their own methods evaluated the PVLs of this reference material as 1.083.49 copies/100 cells. The maximum difference between laboratories was 3.2fold. Correcting measured PVLs by using a formula incorporating the assigned
value of this reference material should minimize such discrepancies.

keyword

Human T-cell leukemia virus type 1; proviral load; quantitative PCR; standard.

※本データの英文表記は実際の論文上の表記とは異なります。