For ad-MED Vitrigel® Series

Cica TEER Measuring System※ TEER : Transepithelial Electro ResistanceTo evaluate
cell barrier function !

Data storageAnalysis

Measurement

Cica TEER Measuring System
ElectrodePC(＊）OS：Windows Vista/7/8.1)※）Electrode and PC are sold separately.

Feature〇 Stable measurement is possible.
〇 Supports data storage and analysis by personal computer.
〇 Compatible with temperature measurement by optional sensor.
〇 Access to inside of the insert is possible while measuring.
〇 Corresponds to eye irritation test method "Vitrigel®-EIT method"
Product No.

Product nameQty. / Pk.

Description49073-00

Cica TEER Measuring System

11 CH

49073-01

Electrode for Cica TEER Measuring System

1For 12 wells, 1CH

Stable measurement is possible.
We developed special electrode corresponding to ad-MED Vitrigel® series. Firmly into theinsert by ﬁxing, small changes in TEER can be accurately measured.
Electrode for ad-MED Vitrigel®

Distribution of TEER value
when repeating the same sample 10 times

Other company's Cica TEER

Supports data storage and analysis by personal computer.
It is possible to save and view data to the personal computer by the attached data transfer
software. In addition, it is also possible to process and analyze with spreadsheet software etc.

※Personal computer is required. (OS : Windows Vista/7/8.1)

Compatible with temperature measurement by optional sensor.
TEER value varies depending on the temperature of the measurement object. You canmonitor the temperature change of the sample by installing a temperature sensor
under measurement.

※ Please contact us for the corresponding temperature sensor.

Access to inside of the insert is possible while measuring.
Samples can be added into the insert while measuring the TEER value.
You can monitor the inﬂuence of samples on cells in real time.
Sample added

Time (min)Corresponds to eye irritancy test method "Vitrigel®-EIT method"“Vitrigel®-EIT method” is a test method to judge the presence or absence of eye irritancyof the chemical substance by analyzing the temporal change of the TEER value ofcorneal model in ad-MED Vitrigel® after dropped it.
It is used for safety evaluation of cosmetics, shampoo and eye drops.
【Method】① Preparing human corneal epithelium model.
Day 0 Seeding of Human corneal epithelial cell (HCE-T) on ad-MED Vitrigel®.
Day 2 Starting Air - liquid interface cultivating
Day 6 Conpleteing Human corneal epithelium model.

Air phase culture

Air - liquid interface culture

Staining image of cross-sections
HE staining and fluorescence immunostaining image of human corneal epithelium model.
ZO-1：Tight junction protein-1
MUC1：Cell surface associated mucin
Keratin K3：Type II cytokeratin found in the corneal epithelium together②Exposuring test substance to cornea model. ③Measuring TEER value for 3 minutes.
Eye irritation
NoneWeakMiddleHigh【References】•••

Time (min)Takezawa T, Nishikawa K, Wang PC. 2011. Development of a human corneal epithelium
model utilizing a collagen vitrigel membrane and the changes of its barrier function induced
by exposing eye irritant chemicals. Toxicol. in Vitro. 25, 1237 -1241.
Yamaguchi H, Kojima H, Takezawa T. 2013. Vitrigel -eye irritancy test method using HCE-Tcells. Toxicol Sci . 135, 347 -55.
Yamaguchi H, Kojima H, Takezawa T. Predictive performance of the Vitrigel -eye irritancy
test method using 118 chemicals. J Appl Toxicol . [Epub ahead of print]Related products
ad-MED Vitrigel®

Cell culture insert
using a collagen Vitrigel® membrane
appropriate for reconstruction of tissue models.

Feature〇 Collagen Vitrigel® membrane composed of high-density collagen ﬁbrils
equivalent to connective tissues.
〇 Appropriate for reconstruction of tissue models.
〇 Excellent visibility for microscopy.
〇 Designs for easier handling.
〇 High adhesive property of animal cells.
〇 Two diﬀerent types of cells are able to culture on the both surface.
Product No.
08360-96

Product Name
ad-MED

Qty. / Pk.

Vitrigel®

１Kit（12wells）

Sterilize with γ-rays in 12-well multiwell plate
Vitrigel® is a registered trademark of National Agriculture and Food Research Organization (NARO).
This product is supported by Agri-Health Translational Research Project from the Ministry of Agriculture,
Foresty and Fisheries of Japan.

Medium for corneal model
Feature〇 Ready-to-Use (supplemented with all necessary supplements)〇 Optimized for corneal modeling using HCE-T cells.
Product No.

Product Name

Qty. / Pk.

25997-96

Medium for corneal model

200 mL

KANTO CHEMICAL CO., INC.
REAGENT DIVISION

East Muromachi Mitsui BLDG, 2-1, Nihonbashi Muromachi 2-chome,
Chuo-ku, Tokyo, 103-0022, JAPAN
Telephone+81-3-6214-1092
Telefax+81-3-3241-1053