Publications
1. Takase M, Iida R, Maruya M, Sakaue-Sawano A, Miyawaki A, Wakayama T, Nishigami S,Fagarasan S, Kanagawa O. Nuclear transferred embryonic stem cell for analysis of B1
B-lymphocyte development. Int Immunol. Int Immunol. 2013 Mar;25(3):145-56. doi:
10.1093/intimm/dxs095.
2. Wakayama S, Kohda T, Obokata H, Tokoro M, Li C, Terashita Y, Mizutani E, Nguyen VT,Kishigami S, Ishino F, Wakayama T Successful Serial Recloning in the Mouse over Multiple
Generations. Cell Stem Cell 2013 Mar 7;12(3):293-7. doi: 10.1016/j.stem.2013.01.005. (Coverarticle of issue)
3. Lee AR, Kishigami S, Amano T, Matsumoto K, Wakayama T, Hosoi Y Nicotinamide: a Class III
HDACi Delays In Vitro Aging of Mouse Oocytes. J Reprod Dev. 2013 Mar 10. [Epub ahead ofprint]
4. Kohda T, Kishigami S, Kaneko-Ishino T, Wakayama T, Ishino F.Gene Expression Profile
Normalization in Cloned Mice by Trichostatin A Treatment. Cell Reprogram. 2012 14：45-55
DOI: 10.1089/cell.2011.0062
5. Mizutani E, Yamagata K, Ono T, Akagi S, Geshi M, Wakayama T. Abnormal chromosome
segregation at early cleavage is a major cause of the full-term developmental failure of mouse clones.
Dev Biol. 2012 2012 364:56-65 doi:10.1016/j.ydbio.2012.01.001
6. Yamagata K, Iwamoto D, Terashita Y, Li C, Wakayama S, Hayashi-Takanaka Y, Kimura H, Saeki K,Wakayama T. Fluorescence cell imaging and manipulation using conventional halogen lamp
microscopy.

PLoS One. 2012;7(2):e31638 doi:10.1371/journal.pone.0031638

7. Terashita Y, Wakayama S, Yamagata K, Li C, Sato E, Wakayama T. Latrunculin A Can Improve theBirth Rate of Cloned Mice and Simplify the Nuclear Transfer Protocol by Gently Inhibiting Actin
Polymerization. Biol Reprod. 2012 86：180 1-6 doi: 10.1095/ biolreprod.111.098764
8.

Nagashimada M, Ohta H, Li C, Nakao K, Uesaka T, Brunet JF, Amiel J, Trochet D, Wakayama T,Enomoto H. Autonomic neurocristopathy-associated mutations in PHOX2B dysregulate Sox10
expression. J Clin Invest. 2012 Sep 4;122(9):3145-58. doi: 10.1172/JCI63401.

9.

Takiguchi M, Kazuki Y, Hiramatsu K, Abe S, Iida Y, Takehara S, Nishida T, Ohbayashi T,Wakayama T, and Oshimura M. A Novel and Stable Mouse Artificial Chromosome Vector. ACS

Synthetic Biology 2012, October 9 ASAP. DOI: 10.1021/sb3000723
10. Itoi F, Tokoro M, Terashita Y, Yamagata K, Fukunaga N, Asada Y, Wakayama T. Offspring frommouse embryos developed using a simple incubator-free culture system with a deoxidizing agent.
PLoS One. 2012;7(10):e47512. doi: 10.1371/journal.pone.0047512. Epub 2012 Oct 9.
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11. Bui HT, Wakayama S, Mizutani E, Park KK, Kim JH, Van Thuan N, Wakayama T. Essential role ofpaternal chromatin in the regulation of transcriptional activity during mouse preimplantation
development. Reproduction. 2011 141:67-77.
12. Murakami K, Araki K, Ohtsuka S, Wakayama T, Niwa H. Choice of random rather than imprinted
X inactivation in female embryonic stem cell-derived extra-embryonic cells. Development. 2011
138:197-202.
13. Maruya M, Suzuki K, Fujimoto H, Miyajima M, Kanagawa O, Wakayama T, Fagarasan S. Vitamin
A-dependent transcriptional activation of the nuclear factor of activated T cells c1 (NFATc1) iscritical for the development and survival of B1 cells. Proc Natl Acad Sci U S A. 2011 108:722-727.
14. Hirata S, Fukasawa H, Wakayama S, Wakayama T, Hoshi K. (2011) Generation of Healthy Cloned
Mice using Enucleated Cryopreserved Oocytes. Cellular Reprogramming 2011、13：7-11.
15. Ono T, Mizutani E, Li C, Yamagata K, Wakayama T. Offspring from intracytoplasmic sperm
injection of aged mouse oocytes treated with caffeine or MG132. Genesis. 2011 49: 460-471 DOI:
10.1002/dvg.20756
16. Amarnath D, Choi I, Moawad AR, Wakayama T, Campbell KH. Nuclear-cytoplasmic
incompatibility and inefficient development of pig-mouse cytoplasmic hybrid embryos.
Reproduction. 2011 142: 295-307 DOI: 10.1530/REP-11-0044
17. Hayashi-Takanaka Y, Yamagata K, Wakayama T, Stasevich TJ, Kainuma T, Tsurimoto T,Tachibana M, Shinkai Y, Kurumizaka H, Nozaki N, Kimura H. Tracking epigenetic histone
modifications in single cells using Fab-based live endogenous modification labeling. Nucleic Acids
Res. 2011 39: 6475-6488. doi: 10.1093/nar/gkr343
18. Cui, XS., Shen, XH., Lee CK., Kang, YK., Wakayama, T. Kim NH. Analysis of proteomic
profiling of mouse embryonic stem cells derived from fertilized, parthenogenetic and androgenetic
blastocysts. Stem Cell Discovery 1: 1-15 (2011).
19. Terashita Y, Li C, Yamagata K, Sato E, Wakayama T. Effect of Fluorescent Mercury Light
Irradiation on In Vitro and In Vivo Development of Mouse Oocytes after Parthenogenetic Activationor Sperm Microinjection. J Reprod Dev. 2011 57: 564-571 doi:10.1262/jrd.11-015H (Cover articleof issue)
20. Kohda T, Ogonuki N, Inoue K, Furuse T, Kaneda H, Suzuki T, Kaneko-Ishino T, Wakayama T,Wakana S, Ogura A, Ishino F. Intracytoplasmic sperm injection induces transcriptome perturbation2

without any transgenerational effect. Biochem Biophys Res Commun. 2011 410: 282-288
doi:10.1016/j.bbrc.2011.05.133
21. Amarnath D, Wakayama S, Zhu J, Moawad AR, Wakayama T, Campbell KH The novel use ofmodified pig zygotic medium for the efficient culture of the preimplantation mouse embryos.
Theriogenology. 2011 76: 1639-1646

http://dx.doi.org/10.1016/j.theriogenology.2011.06.028,

22. Li C, Mizutani E, Ono T, Terashita Y, Jia XF, Shi HJ, Wakayama T. Intracytoplasmic Sperm
Injection with Mouse Spermatozoa Preserved Without Freezing for Six Months Can Lead toFull-Term Development. Biol Reprod. 2011 85:1183-1190 DOI 10.1095/biolreprod.111.091827
23. Bui HT, Seo HJ, Park MR, Park JY, Van Thuan N, Wakayama T, Kim JH. Histone Deacetylase
Inhibition Improves Activation of Ribosomal RNA Genes and Embryonic Nucleolar
Reprogramming in Cloned Mouse Embryos. Biol Reprod. 2011 85: 1048-1056 DOI10.1095/biolreprod.110.089474
24. Li, C. Mizutani, E. Ono, T. Wakayama, T. (2010) An Efficient Method for Generating Transgenic
Mice Using NaOH-Treated Spermatozoa. Biol Reprod. 82: 331-340
25. Ono T, Mizutani E, Chong L, Wakayama T. (2010) Preservation of sperm within the mouse cauda
epididymidis in salt or sugars at room temperature. Zygote 18:245-256
26. Okada, Y. Yamagata, K. Hong, K. Wakayama, T. Zhang, Y. (2010) A role for the elongator complex
in zygotic paternal genome demethylation. Nature 463: 554-558
27. Dahl JA, Reiner AH, Klungland A, Wakayama T, Collas P. (2010) Histone H3 lysine 27 methylation
asymmetry on developmentally-regulated promoters distinguish the first two lineages in mouse
preimplantation embryos. PLoS One. 5:e9150.
28. Sato S, Yagi S, Arai Y, Hirabayashi K, Hattori N, Iwatani M, Okita K, Ohgane J, Tanaka S,Wakayama T, Yamanaka S, Shiota K. (2010) Genome-wide DNA methylation profile oftissue-dependent and differentially methylated regions (T-DMRs) residing in mouse pluripotent
stem cells. Genes Cells. 15: 607-618.
29. Bui H.T., Wakayama S., Kishigami S., Kim HJ., Van Thuan N., and Wakayama T. (2010) Effect ofTrichostatin A on chromatin remodeling, histone modifications, DNA replication and transcriptional
activity in cloned mouse embryos. Biol Reprod. 83: 454-463.
30. Sakaue M, Ohta H, Kumaki Y, Oda M, Sakaide Y, Matsuoka C, Yamagiwa A, Niwa H, Wakayama
T, Okano M. (2010) DNA Methylation Is Dispensable for the Growth and Survival of theExtraembryonic Lineages. Curr Biol. 20: 1452-1457.
31. Hikichi T., Ohta H., Wakayama S., and Wakayama T. (2010) Functional full-term placentas formed
from parthenogenetic embryos using serial nuclear transfer. Development 137: 2841-2847.
332. Yamagata K, Ueda J, Mizutani E, Saitou M, Wakayama T. (2010) Survival and death of epiblast
cells during embryonic stem cell derivation revealed by long-term live-cell imaging with an Oct4
reporter system. Dev Biol. 346: 90-101.
33. Ono T., Li C., Mizutani E., Terashita Y., Yamagata K., and Wakayama T. (2010) Inhibition of ClassIIb Histone Deacetylase Significantly Improves Cloning Efficiency in Mice. Biol Reprod.
83:929-937
34. Kim HR, Han RX, Wakayama T, Park CS, Jin DI. （2010） Aberrant protein expression in theplacenta of cloned mouse derived from embryonic stem cell. Placenta 31:853-859.
35.
Kai Y, Wang CC, Kishigami S, Kazuki Y, Abe S, Takiguchi M, Shirayoshi Y, Inoue T, Ito H,Wakayama T, and Oshimura M.(2009). Enhanced apoptosis during early neuronal differentiation inmouse ES cells with autosomal imbalance. Cell. Res. 19: 247-258.
36.
Ohta H, Sakaide Y, and Wakayama T. (2009).Functional Analysis of Male Mouse Haploid
Germ Cells of Various Differentiation Stages: Early and Late Round Spermatids Are Functionally
Equivalent in Producing Progeny. Biol. Reprod. 80: 511-517.
37.
Ohta H, Sakaide Y, and Wakayama T. (2009). Age- and substrain-dependent sperm
abnormalities in BALB/c mice and functional assessment of abnormal sperm by ICSI. Hum.
Reprod. 24:775-781
38.
Li C, Mizutani E, Ono T, and Wakayama T. (2009) Production of normal mice from
spermatozoa denatured with high alkali treatment before ICSI. Reproduction 137:779-792
39. Yamagata K, Suetsugu R, Wakayama T. (2009) Long-term, Six-dimensional Live-cell Imaging for
the Mouse Preimplantation Embryo That Does Not Affect Full-term Development. J Reprod Dev.
55:343-350.
40. Bui HT, Hwang KC, Kim JH, VAN Thuan N, Wakayama T, Miyano T. (2009) Effect of Vanadateon the Chromatin Configuration in Pig GV-oocytes. J Reprod Dev. 55：367-372
41. Ohinata Y, Ohta H, Shigeta M, Yamanaka K, Wakayama T, Saitou M. (2009) A signaling principle
for the specification of the germ cell lineage in mice. Cell. 137:571-584.
42. Van Thuan N, Bui HT, Kim JH, Hikichi T, Wakayama S, Kishigami S, Mizutani E, Wakayama T.
(2009) The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues
full-term development in cloned inbred mice. Reproduction., 138:309-317
43. Yamagata K, Suetsugu R, Wakayama T. (2009) Assessment of chromosomal integrity using a novel
live-cell imaging technique in mouse embryos produced by intracytoplasmic sperm injection. Hum.
Reprod., 24: 2490-2499.
44. Wakayama S, Kawahara Y, Li C, Yamagata K, Yuge L and Wakayama T. (2009) Detrimental
Effects of Microgravity on Mouse Preimplantation Development in Vitro. PloS ONE, 4: e6753
1-10.
445. Ohta, H. Ohinata, Y. Ikawa, M. Morioka, Y. Sakaide, Y. Saitou, M. Kanagawa, O. Wakayama, T.
(2009) Male Germline and Embryonic Stem Cell Lines from NOD Mice: Efficient Derivation of GS
Cells from a Nonpermissive Strain for ES Cell Derivation Biol. Reprod. 81: 1147-1153.
46. Oda, M. Tanaka, S. Yamazaki, Y. Ohta, H. Iwatani, M. Suzuki, M. Ohgane, J. Hattori, N.
Yanagimachi, R. Wakayama, T. Shiota, K. (2009) Establishment of trophoblast stem cell lines fromsomatic cell nuclear-transferred embryos Proc Natl Acad Sci U S A. 106：16293-16297
47.
Wakayama S, Kishigami S, Thuan NV, Ohta H, Hikichi T, Mizutani E, Bui HT, Miyake M, andWakayama T. (2008) Effect of volume of oocyte cytoplasm on embryo development afterparthenogenetic activation, intracytoplasmic sperm injection, or somatic cell nuclear transfer.
Zygote 16:211-222.
48.
Hikichi T, Kohda T, Wakayama S, Ishino F, and Wakayama T. (2008)Nuclear Transfer Alters
the DNA Methylation Status of Specific Genes in Fertilized And Parthenogenetically ActivatedMouse Embryonic Stem Cells. Stem Cells. 26: 783-788.
49.
Ohta H, Sakaide Y, and Wakayama T. (2008) The Birth of Mice from Testicular Spermatozoa
Retrieved from Frozen Testicular Sections. Biol Reprod 78: 807-811.
50.
Tabar V, Tomishima M, Panagiotakos G, Wakayama S, Menon J, Chan B, Mizutani E,Al-Shamy G, Ohta H, Wakayama T, and Studer L.(2008) Therapeutic cloning in individual
parkinsonian mice. Nat. Med. 14: 379-381
51.
Shibata S, Yokota T, and Wakayama T.(2008) X-chromosome inactivation in nuclear transfer
ES cells. Cytogenetic and Genome Research 121:96-101
52.
Suzuki T, Kondo S, and Wakayama T, Cizdziel PE, and Hayashizaki Y. (2008) Genome-WideAnalysis of Abnormal H3K9 Acetylation in Cloned Mice. PLoS One 3:e1905.
53.
Ohta H, Sakaide Y, and Wakayama T. (2008) Long-Term Preservation of Mouse Spermatozoa
as Frozen Testicular Sections J. Reprod. Dev. 54:295-298
54.
Ohta H, Sakaide Y, Yamagata K, and Wakayama T.(2008) Increasing the Cell Number of Host
Tetraploid Embryos Can Improve the Production of ES Mice. Biol. Reprod. 2008 79:486-492.
55.
Mizutani E, Ono T, Li Chong, Maki-Suetsugu R, Wakayama T. (2008) Propagation ofsenescent mice using nuclear transfer embryonic stem cell lines. Genesis 46; 478-483
56.
Ohta H., Sakaide Y., Wakayama T. (2008) Generation of ES mice using mouse blastocysts ofdifferent developmental ages. Reproduction 136:581-587
57.
Ono T., Mizutani E., Li C., Wakayama T. (2008) Nuclear transfer preserves the nuclear genomeof freeze-dried mouse cells. J. Reprod Dev. 54: 486-491.
58.
Bui HT., Wakayama S., Kishigami S., Thuan NV. Wakayama T. (2008) The cytoplasm ofmouse germinal vesicle (GV) stage oocytes can enhance somatic cell nuclear reprogramming.
Development 135:3935-3945.
59.
Wakayama S, Ohta H, Hikichi T, Mizutani E, Iwaki T, Kanagawa O, Wakayama T (2008)5

Production of healthy cloned mice from bodies frozen at -20oC for 16 years, Proc Natl Acad Sci U S
A. 105, 17318–17322.
60.
Nishida H, Kondo S, Suzuki T, Tsujimura Y, Komatsu S, Wakayama T, Y Hayashizaki (2008)
An epigenetic aberration increased in intergenic regions of cloned mice. Mammalian Genome 19:667-674.

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