Cell culture insert

ad-MED Vitrigel™ series

ad-MED Vitrigel™ 2 is a cell culture insert using collagen VitrigelTM membrane that is composed ofonly bovine collagen. ad-MED Vitrigel™ 2 is suitable for various applicationssuch as permeability
assay, construction of tissue model, and co-culture of different type of cells.

Collagen VitrigelTM
Collagen solution forms a gel depending on pH, temperature, and ionic strength conditions.
When the moisture of the collagen gel is removed, the collagen fiber density increases and the collagen gelturns into a film. Because this dehydration process is called “vitrification”, the film produced by this method iscalled “VitrigelTM”.
Collagen gel

Collagen Vitrigel™

Vitrification

Visual appearance

SEM image

Visual appearance

SEM image

Vitrigel is a registered trademark of National Agriculture and Food Research Organization (NARO).
This product is supported by Agri-Health Translational Research Project from the Ministry of Agriculture,
Foresty and Fisheries of Japan.

KANTO CHEMICAL CO., INC.

Vitrigel™ membrane

１．Membrane structure
The PET membrane commonly used for a cell culture insert has a pore structure. On the other hand,collagen VitrigelTM membrane is composed of high density collagen fibers which have a collagen-specificstripe structure.
【SEM image】

1.0 µmOther company’s product A（PET, 0.4 µm）

Other company’s product B（PTFE）

ad-MED Vitrigel™ 2（Collagen VitrigelTM membrane）

２．Transparency
These images indicate transparency of each membranes. Each cell culture inserts were placedon the black cloth after rehydration with PBS. Transparency of collagen VitrigelTM membrane is higher
than that of PET or PTFE membrane.

Other company’s product A（PET, 0.4 µm）

Other company’s product B（PTFE）

ad-MED Vitrigel™ 2（Collagen VitrigelTM membrane）

３．Low fluorecent membrane
Collagen Vitrigel™ membrane is low fluorescent membrane. It makes high-contrast fluorescent observation.

Cell：Caco-2
Blue：Nucleus

Other company’s product A（PET, 0.4 µm）

2ad-MED Vitrigel™ 2（Collagen Vitrigel™ membrane）

ad-MED Vitrigel™ 2４．Permeability
These are results of permeability test of collagen VitrigelTM membrane and PET（1.0 or 0.4 μm）membrane.
FITC-dextran (MW:4,000 or 10,000 or 40,000) was used as a permeable substance.These results
suggested that the permeability of collagen VitrigelTM membrane was higher than that of PET (0.4 μm)
membrane and dependent on the molecular weight of a substance unlike the PET membrane.

FD: FITC-dextran
FD4: MW 4,000
FD10: MW 10,000
FD40: MW 40,000

５．Designs for easier handling

Pipette end

Phase contrast microscopic observation of NIH3T3 cells after
medium change. Cell layer is completely maintained in
ad-MED Vitrigel™ (left). On the other hands, cells are unstuck
in a insert without channel-like structure (right) .

Channel-like structure that assist pipette handling

６．Constructing co-culture systemad-MED Vitrigel™ 2 allows for co-culturing different cell types on both inner and outer side ofcollagen Vitrigel™ membrane by using option ring.

Liquid phasecultureAir-liquid interface culture

1. Set a option ring to
ad-MED Vitrigel™ 2

2. Seed cells to back side

Ordinary
co-culture

Co-cultureusing option ring

3. Remove a option ring and set
ad-MED Vitrigel™ 2 in a multi-well plate

3Vitrigel™ membrane
We have the technology to process Vitrigel™ membrane into various form.
If you nedd more infromation about Vitrigel™ membrane, please contact us.
ex. 1）Circular membrane（φ15 ㎜、φ21 ㎜）
φ15 ㎜ is equivalent to the bottom ofad-MED Vitrigel™ 2 (24 well), φ21 ㎜ is
ad-MED Vitrigel™ 2 (12 well) too.

ex. 2）Square membrane（104 ㎜×74 ㎜）
examples of custom-made products.

＊This product is research use only.
Do not use for medical or diagnostic purposes.

Product information
Product Name

Package

Product No.

ad-MED Vitrigel™ 2（12 well）

12 wells / plate08363-96

ad-MED Vitrigel™ 2（24 well）

24 wells / plate

08364-96

ad-MED Vitrigel™ 2（96 well）

96 wells / plate

08368-96

Option ring for ad-MED Vitrigel™（12 well）

24 pieces / pack08369-96

Option ring for ad-MED Vitrigel™（24 well）

24 pieces / pack08373-96

1 Set*1

32448-67

Cell culture insert ad-MED Vitrigel™ Series

Reservoir plate set for ad-MED Vitrigel™ 2 (96 well)*1

Reservoir plate set includes single well plate, 96-well plate, insert guide, rid.

Product NamePackage

Product No.

Vitrigel 2 membrane (φ21 ㎜), sterile

24 pieces44126-67Vitrigel 2 membrane (φ15 ㎜), sterile

24 pieces

44125-67

MembraneBBz-26-ENG (202303)