http://support.spring8.or.jp/Report_JSR/PDF_IAe_ALL/IAe_11A1744.pdf

2011A1744                          BL40XU೺඲࡞ṑ࢚ࢼ࣓ࣝ㉁ࡢṑ㉁ᙉ໬࡟ࡼࡿ⤖ᬗᵓ㐀ኚ໬ࡢ᳨ド
A Wide-angle Diffraction Analyses for Enhanced Tooth Enamel Subsurface⏣୰ ᬛᏊ a, 㔩㜰 ᐶ a, 㕥ᮌ኱௓ a, ྜྷᯇ኱௓ a, ᑠᯘ㝯Ⴙ a,ඵᮌ ┤ே b,ኴ⏣᪼ b,㣤ᓥὒ୍ ca
Tomoko Tanaka , Hiroshi Kamasakaa, Daisuke Suzukia, Daisuke Yoshimatsua, Takatsugu Kobayashia,
Naoto Yagib, Noboru Otab, Youichi IijimacaỤᓮࢢࣜࢥ㻔ᰴ㻕೺ᗣ⛉Ꮫ◊✲ᡤ, b㻔බ㈈㻕㧗㍤ᗘග⛉Ꮫ◊✲ࢭࣥࢱ࣮, c 㛗ᓮ኱Ꮫa
Health Science Laboratory, Ezaki Glico Co.,Ltd,䢢bJapan Synchrotron Radiation Research Institute,cNagasaki Universityࣜࣥ㓟໬࢜ࣜࢦ⢾࢝ࣝࢩ࣒࢘㻔POs-Ca®㻕ࡣึᮇ࠺⼃࡟࠾ࡅࡿ෌▼⅊໬ࢆಁ㐍ࡍࡿࠋࡇࢀࡲ࡛ࡢ◊✲࡟ࡼࡾࠊPOs-Ca ࡟ࡼࡗ࡚෌▼⅊໬ࡋࡓ㒊఩࡛ࡣ೺඲࡞࢚ࢼ࣓ࣝ㉁࡜ྠᵝࡢ㓄ྥᛶࢆᣢࡗࡓࣁ࢖
ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺ㻔HAp㻕⤖ᬗࡀᅇ᚟ࡋ࡚࠸ࡿࡇ࡜ࡀ᫂ࡽ࠿࡟࡞ࡗࡓࠋᮏ◊✲࡛ࡣࠊSPring-8
BL40XU ࡟࠾ࡅࡿᗈゅ X ⥺ᅇᢡゎᯒ࡟ࡼࡾࠊ࠺⼃ண㜵ຠᯝࡀ㧗࠸ࡇ࡜ࡀ▱ࡽࢀ࡚࠸ࡿࣇࢵ໬≀㻔ᴟప⃰ᗘ㻕࡜ POs-Ca ࡢే⏝ࡀࠊᏛ❺ᮇࡢࣄࢺṑ࢚ࢼ࣓ࣝ㉁ࡢ⤖ᬗ໬࡟ᙳ㡪ࢆཬࡰࡍࡇ࡜ࢆ᫂ࡽ࠿࡟ࡋࡓࠋ࣮࣮࢟࣡ࢻ㸸 ึᮇ࠺⼃ࠊࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺࠊ෌▼⅊໬ࠊ࢚ࢼ࣓ࣝ㉁ࠊࣇࢵ໬≀⫼ᬒ࡜◊✲┠ⓗ㸸࠺⼃ࡣࠊே㢮ࡢ QOL㻔quality of life㻕࡟኱ࡁࡃᙳ㡪ࢆ୚࠼ࡿ⑌ᝈࡢ 1 ࡘ࡛࠶ࡿࠋ࠺⼃ࡣࠊ࠶ࡿ᪥✺↛ᙧᡂࡉࢀࡿࡶࡢ࡛ࡣ࡞ࡃࠊ᪥ࠎࡢ⏕άࡢ୰࡛ᚎࠎ࡟ᙧᡂࡉࢀ࡚࠸ࡃࡶࡢ࡛࠶ࡿࠋ๓ẁ㝵࡜ࡋ࡚ࠊࡲࡎึᮇ࠺⼃ࡀᙧᡂࡉࢀࡿࠋึᮇ࠺⼃ࡣ⑌⑓࡛ࡣ࡞ࡃࠊ࡯࡜ࢇ࡝ࡢேࡢཱྀ⭍ෆ࡟᪥ᖖᖍ࡟Ꮡᅾࡋᚓࡿ࡜࠸࠺ᡤぢࡀ࠶ࡿࠋẖ㣗ᚋࠊ㣗≀䛻ྵ䜎䜜䜛䝅䝵⢾䛺䛹䜢ṑᇈ⣽⳦䛜㈨໬䛧䚸⏘⏕䛩䜛㓟䛻ṑ䜶
䝘䝯䝹㉁䛜㛗ᮇ㛫䛥䜙䛥䜜䛯⤖ᯝ䚸䜶䝘䝯䝹㉁⾲㠃䛾⾲ᒙୗ䛛䜙ṑ䛾ᵓᡂᡂศ䛷䛒䜛䜹䝹䝅䜴䝮䛸䝸䞁㓟䛜ၚᾮ୰䛻⁐䛡䛰䛩⬺⅊䛜㉳䛣䜚䚸ึᮇ䛖⼃䛸䛺䜛䚹୍᪉䛷ึᮇ䛖⼃䛻䜘䜚ṑ䛛䜙ኻ䜟䜜䛯䝭䝛䝷䝹䛿䚸ၚᾮ䛛䜙ṑ䛻ྲྀ䜚ᡠ䛩㻔෌▼⅊໬㻕䛜ྍ⬟䛸䛺䜚ᅇ᚟䛩䜛䛣䛸䛜䛷䛝䜛䚹䛧䛛䛧ึᮇ䛖⼃䛜㐍⾜䛧ᐇ㉁Ḟᦆ䜢క䛳䛯䛖❐ᙧᡂ㻔䛖⼃㻕䜢ᙧᡂ䛧䛶䛧䜎䛖䛸䚸ṑ⛉἞⒪䛺䛧䛻ᅇ᚟䛿୙ྍ⬟䛸䛺䜛䚹೺ᗣయ䛷୍⏕ᾭ䜢㐣䛤䛩
䛯䜑䛾 1 䛴䛾ᡭẁ䛸䛧䛶䚸೺඲ṑ䜢䛔䛛䛻⥔ᣢ䛩䜛䛛䛜㘽䛸䛺䜛䚹䛭䛣䛷䚸Ụᓮࢢࣜࢥ㻔ᰴ㻕࡛ࡣࠊ⮬♫࡛㛤Ⓨࡋࡓ᪂⣲ᮦࡢ㧗Ỉ⁐ᛶ࢝ࣝࢩ࣒࢘ ࣜࣥ㓟໬࢜ࣜࢦ⢾࢝ࣝࢩ࣒࢘ 㻔POs-Ca㻕ࢆ⏝࠸࡚ึᮇ࠺⼃࡟࢔ࣉ࣮ࣟࢳ࡛ࡁࡿᶵ⬟ᛶ࣒࢞ࢆ㛤Ⓨࡋࡓ[1]ࠋ᪥ᖖ⏕άࡢ୰࡛ࠊၚᾮ୰ࡢ࢝ࣝࢩ࣒࢘࢖࢜ࣥ⃰ᗘࢆ㧗ࡵึᮇ࠺⼃ࡢ෌▼⅊໬࣭෌⤖ᬗ໬ຠᯝࢆぢฟࡋࠊ≉ᐃಖ೺⏝㣗ရࡢࢩ࣮ࣗ࢞ࣞࢫ࣒࢞࡜ࡋ୍࡚⯡ᕷሙ࡟ୖᕷࡋࡓࠋࡋ࠿ࡋṑ㉁ࢆᙉ໬ࡍࡿࡓࡵ࡟ࡣࠊ࢝ࣝࢩ࣒࢘ࡔࡅ࡛࡞ࡃ࠺⼃ண㜵ຠᯝࡀ㧗࠸࡜ࡉࢀ࡚࠸ࡿࣇࢵ໬≀[2][3]ࡢ฼⏝ࡀᮃࡲࢀࡿࠋ࡜ࡇࢁࡀᚑ᮶ࠊࣇࢵ⣲ࡣ཯ᛂᛶࡀ㧗࠸ࡓࡵ࢝ࣝࢩ࣒࢘࡜ࡢే⏝ࡣ㞴ࡋ࠿ࡗࡓࠋ࡜ࡇࢁࡀ POs-Ca ࡢࡶࡘ࢝ࣝࢩ࣒࢘ࡣỈ⁐ᛶࡀ㧗ࡃࠊpH ࡀ୰ᛶ⎔ቃ࡟࠾࠸࡚ࡶࣜࣥ㓟࡜ሷࢆᙧᡂࡍࡿࡇ࡜࡞ࡃᏳᐃ࡟Ꮡᅾࡍࡿࡇ࡜࠿ࡽࠊࣇࢵ໬≀࡜ POs-Ca ࢆ⏝࠸࡚ึᮇ࠺⼃࡟ࣉ࣮ࣟࢳ◊✲ࢆ⾜ࡗ࡚ࡁࡓࠋᮏ◊✲࡛ࡣࡑࡢຠᯝ࡟ࡘ࠸࡚ࠊṑ㉁ࡣ⤖ᬗᵓ㐀࡟౫Ꮡࡍࡿ㒊ศࡀ኱ࡁ࠸ࡇ࡜࠿ࡽࠊᚑ᮶ἲ࡛࠶ࡿ transversal microradiography㻔TMR㻕ἲ࡟ࡼࡿ࣑ࢿࣛࣝྵ᭷㔞ࡢホ౯࡟ຍ࠼ࠊWAXRD ࡟ࡼࡿ⤖ᬗᛶ࡟ࡘ࠸࡚ࠊࣄࢺṑ࢚ࢼ࣓ࣝ㉁ࢆ⏝࠸᳨࡚ウࡋࡓ⤖ᯝࢆሗ࿌ࡍࡿࠋᐇ㦂㸸㸺ࢧࣥࣉࣝㄪ〇㸼ṑ࢚ࢼ࣓ࣝ㉁࠿ࡽṑ∦ࢧࣥࣉࣝࢆㄪ〇ࡋࠊ⬺⅊ฎ⌮⾜ࡗࡓࡢࡕ࡟ࠊṑ㉁ᙉ໬ฎ⌮ࢆ⾜࠸ࠊ1ṑ∦ୖ࡟೺඲࣭ṑ㉁ᙉ໬㒊ࠊ⬺⅊㒊ࢆసᡂࡋࡓࡶࡢࢆཌࡉ150 Pm࡟ษࡾฟࡋࠊࢧࣥࣉࣝ࡜ࡍࡿࠋ
෌▼⅊໬ฎ⌮࡟ࡣࠊ⾲࡟♧ࡍࡼ࠺࡟ࠊၚᾮ⤌ᡂ࡟㏆࡙ࡅࡓ࣑ࢿࣛࣝ⁐ᾮ࡟࢝ࣝࢩ࣒࢘㻔POs-Ca ⏤᮶㻕ࡶࡋࡃࡣ࢝ࣝࢩ࣒࢘࡜ࣇࢵ໬≀ࢆᙉ໬ࡋࡓྛ✀࣑ࢿࣛࣝ⁐ᾮ㻔A,B, ⾲1ཧ↷㻕ࢆㄪ〇ࡋࠊ37Υ
24᫬㛫ฎ⌮཯ᛂࢆ⾜࠸ࡑࡢຠᯝࢆẚ㍑ࡋࡓࠋヨᩱ࡟ࡣࠊࣄࢺṑࢆ⏝࠸ࡓࠋヨᩱࡣḟࡢࡼ࠺࡟ㄪ
〇ࡋࡓࠋ㸺 ᐃ㸼
࢚ࢼ࣓ࣝ㉁ⷧ∦ࢧࣥࣉࣝ㻔ཌࡉ150 Pm㻕ㄪ〇ࡉࢀࡓࢧࣥࣉࣝࡣ2ḟඖ࡟⛣ືྍ⬟࡞ヨᩱࢫࢸ࣮ࢪ࡟ࠊ࢚ࢼ࣓ࣝ㉁-㇟∳㉁᪉ྥ 㻔ṑࡢ῝ࡉ᪉ྥ㻕 ࡟ᑐࡋ࡚ᆶ┤࡟ࣅ࣮࣒ࢆᙜ࡚ࡿࡼ࠺࡟ࢭࢵࢺࡋࠊ
X⥺ࢆ↷ᑕࡋࡓ [4]ࠋࣅ࣮࣒ࡣ6 μm⛬ᗘࡢ኱ࡁࡉࡢX⥺ࣅ࣮࣒࡜࡞ࡿࡼ࠺࡟ࠊࣆ࣮ࣥ࣍ࣝࢆ㏻ࡋ
࡚ㄪᩚࡋࡓ15 keV๓ᚋࡢX⥺ࢆ↷ᑕࡋࠊᅇᢡീࢆᚓࡓࠋ࢚ࢼ࣓ࣝ⾲㠃࠿ࡽ῝㒊࡟ྥ࠿ࡗ࡚5 μm
ࡈ࡜࡟῝ࡉ200 μmࡲ࡛⾜ࡗࡓࠋᚓࡽࢀࡓX⥺ᅇᢡࢹ࣮ࢱ࡟ࡘ࠸࡚ࡣࠊHAp⤖ᬗࡢc㍈ࡢ⣽࠿࡞㐪࠸࡟ᙳ㡪ࡉࢀ࡟ࡃ࠸HAp⏤᮶ࡢ㻔100㻕ࡢ཯ᑕ࡟ࡘ࠸࡚ᙉᗘ✚ศࢆ⾜࠸ࠊ೺඲㒊ࡢᙉᗘ࡛┦ᑐ್໬ࡍࡿࡇ࡜࡟ࡼࡗ࡚ࠊྛᐃⅬ࡟࠾ࡅࡿHAp⤖ᬗྵ᭷㔞ࢆ⟬ฟࡋࡓ[4][5] 㻔⤖ᬗࣉࣟࣇ࢓࢖ࣝ㸸ᅗ

1-A, B ཧ↷㻕ࠋ⤖ᬗࡢࣉࣟࣇ࢓࢖ࣝ࠿ࡽᅇ᚟⋡ࢆィ⟬ࡋࡓࠋ࣓࢝ࣛ㛗ࡣヨᩱ࡟ᛂࡌ࡚ㄪᩚࡋࡓࠋ⾲1㸬ྛ✀෌▼⅊໬ฎ⌮ᾮ
A ⁐ᾮ
6 mM
3.6 mM
0 ppm
20 mM
6.5
100 mL

B ⁐ᾮ
6 mM
3.6 mM
1 ppm
20 mM
6.5
100 mL

㻝㻚㻞

㻝㻚㻞

㻝㻚㻜

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㻜㻚㻤

㻜㻚㻤
㻴㻭㼜㻌⤖ᬗ㔞㻴㻭㼜㻌⤖ᬗ㔞⤌ᡂ
Ca
KH2PO4
Fluoride
HEPES
pHTotal

㻜㻚㻢
㻜㻚㻠

㻜㻚㻢
㻜㻚㻠
ึᮇ䛖⼃㒊఩

ึᮇ䛖⼃㒊఩
㻜㻚㻞

㻜㻚㻞

ฎ⌮㒊఩

㻜㻚㻜
㻙㻟㻜

ฎ⌮㒊఩

㻜㻚㻜
㻜

㻟㻜

㻢㻜

㻥㻜

㻝㻞㻜

㻝㻡㻜

㻙㻟㻜

㻜

㻟㻜

㻢㻜

㻥㻜

㻝㻞㻜

䜶䝘䝯䝹㉁⾲㠃䛛䜙䛾῝䛥䚷㻌㻔P㼙㻕䜶䝘䝯䝹㉁⾲㠃䛛䜙䛾῝䛥䚷㻌㻔P㼙㻕

ᅗ 1-A㸬
ᅗ 1. ྛ✀࣑ࢿࣛࣝ⁐ᾮ㻔⁐ᾮ A,B㻕࡛ฎ⌮ࢆࡋࡓሙྜ࡟ᚓࡽࢀࡓ
ᗈゅ X ⥺ᅇᢡ࡟ࡼࡿ࢔ࣃࢱ࢖ࢺ⤖ᬗኚ໬㔞ࣉࣟࣇ࢓࢖ࣝ

ᅗ 1-B㸬㻝㻡㻜⤖ᯝ࠾ࡼࡧ⪃ᐹ㸸X ⥺࣐࢖ࢡࣟࣅ࣮࣒ࢆ⏝࠸ࡓ WAXRD ࡢゎᯒ࠿ࡽࠊࣄࢺṑ࡟ᙧᡂࡉࢀࡓึᮇ࠺⼃࡟࠾࠸࡚ࠊ࢝
ࣝࢩ࣒࢘㻔POs-Ca ⏤᮶㻕࡜ࣇࢵ໬≀㻔F㻕ࡶࡋࡃࡣ࢝ࣝࢩ࣒࢘㻔POs-Ca ⏤᮶㻕࡛ฎ⌮ࢆ⾜ࡗࡓ࠸ࡎࢀ࡟࠾࠸࡚ࡶࠊ೺඲㒊ࡢ࢚ࢼ࣓ࣝ㉁࡜ྠࡌࣁ࢖ࢻࣟ࢟ࢩ࢔ࣃࢱ࢖ࢺ⤖ᬗࡢᅇ᚟ࡀほࡽࢀࡓࠋࡲࡓ࢝ࣝࢩ࣒࢘࡜ࣇࢵ໬≀㻔F㻕࡛ฎ⌮ࢆ⾜ࡗࡓࡶࡢ࡜࢝ࣝࢩ࣒࢘༢⊂ฎ⌮࡜ࡣࠊࡑࡢᅇ᚟ഴྥࡀⱝᖸ␗࡞ࡗ࡚࠸ࡓࠋࡇࡢࡇ࡜࠿ࡽࠊᴟᚤ㔞ࡢࣇࢵ໬≀ࢆῧຍࡍࡿࡇ࡜࡛෌⤖ᬗ໬࡟ᙳ㡪ࡀ୚࠼ࡿࡇ࡜ࡀ♧၀ࡉࢀࡓࠋ௒ᚋࠊ◳ᗘࡸ⪏㓟ᛶ➼ከ㠃ⓗホ౯ࢆຍ࠼࡚ࠊࡑࡢຠᯝ࡟ࡘ࠸࡚ホ౯ࡋࡓ࠸ࠋཧ⪃ᩥ⊩㸸[1] Tanaka M., et al., Effect of chewing gum containing Phosphoryl Oligosaccharides of Calcium 㻔POs-Ca㻕on remineralization and hydroxyapatite crystallites of enamel subsurface lesions in situ. ᪥ᮏṑ⛉ಖᏛ఍ㄅ, vol.52 㻔6㻕㻘 534-542, 㻔2009㻕㻚
[2] Dean H. T., et al., Domestic water and dental caries. Additional studies of the relation of fluoride
domestic waters to dental caries experience in 4,425 while children, aged 12 to 14 years, of 13 cities in
4 States. Pub. Health Rep., 57, 1155-1179, 㻔1942㻕.
[3] Dean H. T., Chronic endemic dental Fluorosis 㻔Mottled Enamel㻕, J. Am. Med. Assoc., 107, 1269-1272,
㻔1936㻕㻚
[4] Yagi N., Ohta N., Matsuo T., Tanaka T., Terada Y., Kamasaka H., To-o K., Kometani T., Kuriki T.,
Evaluation of enamel crystallites in subsurface legion by microbeam X-ray diffraction. J. Synchrotoron
Radiat., 16, 398-404, 㻔2009㻕.
[5] Tanaka T., Yagi N., Ohta N., Matsuo T., Terada Y., Kamasaka H., To-o K., Kometani T., Kuriki T.,
Evaluation of the distribution and orientation of remineralized enamel crystallites in subsurface lesionby X-ray diffraction. Caries Res., 44, 253-259, 㻔2010㻕㻚