The 43rd Annual Meeting of
the Japanese Environmental Mutagen Society (JEMS)“Genotoxicity and Carcinogenesis: Risk Assessment Based on Mechanism of Action”

Date : December 4th – 5th, 2014

Venue : Hitotsubashi Hall, 2-1-2 Hitotsubashi, Chiyoda-ku, Tokyo 101-8439
http://www.hit-u.ac.jp/hall/file/menu-016/file_02.pdf

Yoshifumi Uno, D.V.M., Ph.D.

President

Mitsubishi Tanabe Pharma Corporation

Organizing Commitee

Yoshifumi Uno, Masamitsu Honma, Takeshi Morita, Hiroyuki Kamiya,
Yukari Totsuka, Tsuneo Hashizume, Satoru Ito, Shuichi Hamada,
Yumiko Iwase, Shigeharu Muto, and Eiji Yamamura

ProgramPlenary and Mochizuki Kitashi Award Lecture
・ David Kirkland

1. Novel Approaches for Genotoxic Evaluation– Intelligent Test System
・ Masamitsu Honma
・ Ovanes Mekenyan
・ Scott Auerbach・ Tomonari Matsuda
・ Manabu Yasui

JEMS Award Ceremony and Lectures
・ Tomoe Negishi・ Kazunori Narumi
・ Makoto Hayashi
・ Kazuhiko Takahashi

2. Examples of Genotoxic and/or
Carcinogenic Risk Assessment Based on
Mechanism of Action
・ Arthur. P. Grollman
・ Atsushi Hakura
・ Takashi Umemura
・ Tsuneo Hashizume
・ Masayuki Mishima
・ Yoshifumi Uno

Platform sessions
Poster sessions
Luncheon Seminar・ Brian Burlinson

Exhibitions

BanquetSymposium sessions

December 4th, 7:00 pm startStar Hall, Josui Kaikan

2-1-1 Hitotsubashi, Chiyoda-ku, Tokyo 101-0003

Fee
Registration

BanquetCategory

Early Registration(Until Sept. 30, 2014)

OnsiteRegistration

Category

Early Registration(Until Sept. 30, 2014)

OnsiteRegistration

Member

10,000 JPY

12,000 JPY

Member8,000 JPY

10,000 JPY

Student Member

3,000 JPY

4,000 JPY

Student Member

4,000 JPY

5,000 JPY

Non-member

12,000 JPY

15,000 JPY

Non-member

8,000 JPY

10,000 JPY

Secretariat
In Safety Research Laboratories, Research Division, Mitsubishi Tanabe Pharma Corporation
1-1-1, Kazusakamatari, Kisarazu, Chiba 292-0818

E-mail: secretariat@jems2014.com

December

4 (Thu)〈Hitotsubashi Hall〉

9:30
Registration

10:20 - 10:25〈Hitotsubashi Hall〉

Opening Remarks
Yoshifumi Uno

President of the 43rd JEMS Meeting
Mitsubishi Tanabe Pharma Corporation

10:30 - 12:00〈Hitotsubashi Hall〉

Platform Session 1

Presentation 12min, Discussion 3min

Chairpersons: Takayoshi Suzuki (National Institute of Health Sciences)
Yukari Totsuka (National Cancer Center Research Institute)

O-1（P-004）10:30

1Natl. Cancer Ctr. Res. Inst.Div. Cancer Development System, 2Div.Cancer Genomics,
Dept. Bioinformatics, 4Aichi Med. Univ., Sch. Med., Dept. Publ. Health., 5Tokyo Univ. Agricul,
6
Azabu University		

O-2（P-005）3

10:45

National Cancer Center Research Institute, 2National Cancer Center EPOC, 3Osaka City University

11:00

Germline mutation rate and its threshold in mice
Arikuni Uchimura1, Mayumi Higuchi1, Yohei Minakuchi2, Jo Nishino3, Atsushi Toyoda2,
Asao Fujiyama2, Takeshi Yagi1

1

Graduate School of Frontier Biosciences, Osaka University , 2National Institute of Genetics ,The Institute of Statistical Mathematics

		

O-4

Mutation signature analysis of occupational cholangiocarcinoma anddichrolopropane-exposed cells.
Masanori Goto1, Sachiyo Mimaki2, Shoji Kubo3, Katsuya Tsuchihara2, Hitoshi Nakagama1,
Yukari Totsuka1

1

		

O-3
（P-006）

A multidisciplinary approach to explore the etiology of esophageal cancer in China

Akane Ikeda1, 6, Mamoru Kato2, Tatsuhiro Shibata2, Isao Kurosaka3, Yingsong Lin4,
Yoshitaka Matsushima5, Osamu Endo6, Hitoshi Nakagama1, Yukari Totsuka1

3

11:15

		

Estimation of inherited germline mutations by mouse whole exome sequencing
Kenichi Masumura1, Naomi Toyoda-Hokaiwado1, Yoichi Gondo2, Takehiko Nohmi3,
Masamitsu Honma11		Division of Genetics and Mutagenesis, National Institute of Health Sciences,
RIKEN BioResource Center,
Biological Safety Research Center, National Institute of Health Sciences

2
3

O-5

11:30

		

1

		

O-6
（P-003）		Accumulation characteristics of mutation in descendant mice after the every
generational low dose-rate internal 137Cs radiation exposure, as the Fukushima
simulation experiment.
Hiroo Nakajima1, Yoshiaki Yamaguchi2, Takashi Yoshimura2, Manabu Fukumoto3,
Takeshi Todo1
Dept. Radiat. Biol. Med. Genet., Grad. Sch. Med., Osaka Univ.,
Radioisotope Res. Center, Osaka Univ., 3Dept. Path., Insti. Dev. Aging Cancer., Tohoku Univ.

2

11:45

Risk estimation for airborne particulate matter: comparison with smoking
Masahiko Watanabe, Hiroaki Aso, Katsuya Suemaru

School of Pharmacy, Shujitsu University33

10:30 - 11:45〈Conference Room (1F)〉

Platform Session 2

Presentation 12min, Discussion 3min

Chairpersons: Satoru Itoh (Daiichi Sankyo Co., Ltd.)
Takeshi Morita (National Institute of Health Sciences)

O-7

10:30

		
		

O-8
（P-039）Mitsubishi Tanabe Pharma Corporation

10:45

		

O-9
（P-058）11:00

Induction of mouse colon tumors by colon–mutagenic noncarcinogens in a colitis
model
Naoki Koyama1, Yuki Seki1, Jiro Sonoda1, Shinya Tanahashi2, Satoru Hosokawa1,
Shoji Asakura1, Kyoko Nakano1, Atsushi Hakura1

1

Tukuba Drug Safety, Eisai Co., Ltd., 2Preclinical Safety Research Laboratories, Sunplanet Co.,Ltd

11:15

Integrated analysis of microRNA expression profile and Comet assay in the mouse
liver for correct classification of genotoxic compounds
Hiroyuki Oka1, Takeki Uehara2, Yuji Morikawa1, Hiroyuki Hanafusa1, Koichi Masuno1,
Hirofumi Miyajima1
1

		

O-11
（P-008）

Toxic evaluation of silver nanoparticles using side scattered light in flow cytometry
and phosphorylation of histone H3
Xiaoxu Zhao, Yuko Ibuki
Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka

		

O-10
（P-048）Investigation for mechanism of DNA adduct formation by 2,4- and2,6-diaminotoluene using DNA adductome analysis
Shigeharu Muto, Katsuya Yamada, Kyoko Kato, Tatsuya Kato, Yumiko Iwase,
Koji Takekawa, Yoshifumi Uno

Research Laboratory for Development, Shionogi & Co., Ltd.,
Global Project Management Department, Shionogi & Co., Ltd.

2

11:30

From margin of exposure (MOE) toward internal MOE (iMOE) -Risk assessmentof carcinogen using hemoglobin adductHiroshi Honda, Kenkichi Fujii, Toshio Kasamatsu, Naohiro Ikeda, Naohiro Nishiyama		R&D - Core Technology - Safety Science Research

13:00 - 14:00〈Hitotsubashi Hall〉

General Meeting

14:00 - 15:00〈Hitotsubashi Hall〉

Award Lectures
Chairperson: Yasunobu Aoki (National Institute for Environmental Studies)
JEMS Award 2014

AW

14:00

		
		

Study of mutation induced by UVA or alkylating agents using DrosophilaTomoe Negishi

Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

JEMS Achievement Award 2014

AA

		
		

14:25

Developmental Study of Repeated-Dose Liver Micronucleus Assay Using Adult
Rats
Kazunori Narumi

Yakult Honsha Co., Ltd.

34JEMS Service Award 2014

SA-1

14:40

		
		

SA-2

Contribution to Internationalization of Genotoxicity Testing in Regulatory Science
Makoto Hayashi

Public Interest Incorporated Foundation BioSafety Research Center

14:50

		

Contribution toward fostering young scientists through the Mutagenesis Research
Meetings
Kazuhiko Takahashi		Yokohama College of Pharmacy

15:10 - 17:40〈Hitotsubashi Hall〉

Symposium 1

Novel Approaches for Genotoxic Evaluation– Intelligent Test System
Chairpersons: Masamitsu Honma (National Institute of Health Sciences)
Eiji Yamamura (Mitsubishi Tanabe Pharma Corporation)

S1-1

15:10

		
		

S1-2

Division of Genetics and Mutagenesis, National Institute of Health Sciences

15:40

		
		

S1-3

16:10

		

Hypothesis generation through holistic analysis of Tox21 data
Scott S Auerbach

Biomolecular Screening Branch, Division of the National Toxicology Program at NIEHS, US

16:40

		
		

S1-5

Mechanism based QSAR for Predicting Mutagenicity and Carcinogenicity
Ovanes Mekenyan

Laboratory of Mathematical Chemistry, Asen Zlatarov University, Bourgas, Bulgaria

		

S1-4

Intelligent Test System in Genetic Toxicology
Masamitsu Honma

DNA adductome for genotoxic evaluation
Tomonari Matsuda

Research Center for Environmental Quality Management, Kyoto University

17:10

		

Site-specific DNA adduct mutagenesis in vivo
Manabu Yasui		Division of Genetics and Mutagenesis, National Institute of Health Sciences

17:45 - 18:45〈Conference Room (2F)〉

Poster Session

19:00 - 20:30

Core time for odd numbers〈Star Hall, 2F Josui Kaikan〉

Banquet35

December

5 (Fri)〈Hitotsubashi Hall〉

9:30
Registration

10:30 - 11:45〈Hitotsubashi Hall〉

Platform Session 3

Presentation 12min, Discussion 3min

Chairpersons: Hiroyuki Kamiya (Graduate School of Biomedical & Health Sciences, Hiroshima University)
Tatsuo Nunoshiba (Graduate School of Arts and Sciences, International Christian University)

O-12（P-073）

10:30

		

O-13

Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka

10:45

		

Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University,
Graduate School of Nutritional and Environmental Sciences, University of Shizuoka,
3
Division of Pharmaceutical Sciences ,Shujitsu University
2

11:00

		

O-15
（P-071）

Numerical genotoxic effects of clustered 8-oxoguanine DNA adducts locallyintroduced into the genome
Akira Sassa, Nagisa Kamoshita, Yuki Kanemaru, Masamitsu Honma, Manabu Yasui

Division of Genetics and Mutagenesis, National Institute of Health Sciences

11:15

Oxidative stress-induced intestinal tumors in Mutyh deficient mice

Mizuki Ohno1, Noriko Takano1, Fumiko Sasaki1, Kenichi Taguchi2, Yusaku Nakabeppu3, 4,
Yasunobu Aoki5, Takehiko Nohmi6, Yoshimichi Nakatsu1, Teruhisa Tsuzuki1
1

		

O-16

Somatic cell mutations caused by 365-nm LED-UVA
Xing Fan1, Sakae Arimoto-Kobayashi1, Tatsushi Toyooka2, Yuko Ibuki2, Xiaoxu Zhao2,
Toshinori Suzuki3, Tomoe Negishi1
1

		

O-14
（P-017）

Formaldehyde delays the repair of pyrimidine dimers and enhances the sensitivity
to UV
Guang Yang, Yuko Ibuki

Dept. of Medical Biophysics and Radiation Biology, Faculty of Medical Sciences, Kyushu University,
Cancer Pathology Lab., National Hospital Organization Kyushu Cancer Center,
3
Division of Neurofunctional Genomics, Medical Institute of Bioregulation, Kyushu University,
4
Research Center for Nucleotide Pool, Kyushu University,
5
Center for Environmental Risk Research, National Institute for Environmental Studies,
6
Division of Genetics and Mutagenesis, National Institute of Health Sciences
2

11:30

		

5caC forms G•T mismatch mimicking-base pairs
Isao Kuraoka, Toshihiro Shibutani, Shigenori Iwai		

Graduate School of Engineering Science Osaka University

12:00 - 12:55〈Conference Room (1F)〉

Luncheon Seminar
Chairperson: Madoka Nakajima (University of Shizuoka)

LS

		
		

12:00

The comet assay in practice; how to get good data from bad tissues
Brian Burlinson

Safety Assessment, Huntingdon Life Sciences36

13:00 - 14:00〈Conference Room (2F)〉

Poster Session

14:00 - 14:45

Core time for even numbers〈Hitotsubashi Hall〉

Special Lecture Kitashi Mochizuki Award
Chairperson: Yoshifumi Uno (Mitsubisi Tanabe Pharma Corporation)
Presenter: Yasunobu Aoki (National Institute for Environmental Studies)PL

Mammalian cell results with Ames-positive chemicals – correlations with presenceor absence of in vivo genotoxic or carcinogenic activity
David Kirkland

14:00

		
		

Kirkland Consulting, UK

14:55 - 17:30〈Hitotsubashi Hall〉

Symposium 2

Examples of Genotoxic and/or Carcinogenic Risk
Assessment Based on Mechanism of Action
Chairpersons: Keiji Wakabayashi (University of Shizuoka)
Yoshifumi Uno (Mitsubishi Tanabe Pharma Corporation)

S2-1

Keynote Lecture
Mutational signature of aristolochic acid as a unique biomarker of human cancer
Arthur P Grollman

14:55

		
		

S2-2

Depts of Pharmacological Sciences and Medicine, Stony Brook University, Stony Brook, NY, USA

Mechanism of colonic cancer induction in mice by colon-mutagenic
noncarcinogens in a dextran sulfate sodium colitis model
Atsushi Hakura

15:25

		
		

S2-3

Tsukuba Drug Safety, Eisai Co., Ltd.

Approach to the elucidation of chemical mutagenesis and carcinogenesis using apathological perspective
Yuji Ishii

15:50

		
		

S2-4

Division of Pathology, National Institute of Health Sciences

Genotoxic (mutagenic) and carcinogenic assessment for impurities inpharmaceuticals
Tsuneo Hashizume

16:15

		
		

S2-5

Takeda Pharmaceutical Company Limited, Pharmaceutical Research Division, Drug Safety ResearchLaboratories

Translation of experimental threshold into human safe dose
Masayuki Mishima

16:40

		
		

S2-6

Chugai Pharmaceutical Co., Ltd., Research Division

Human risk assessment based on genotoxic and/or carcinogenic mechanism ofactionYoshifumi Uno

17:05

		
		

Mitsubishi Tanabe Pharma Co., Research Division, Safety Research Laboratories

17:30 - 17:40〈Hitotsubashi Hall〉

Closing Remarks
37

Poster Presentation
Poster View Time: December 4 (Thu)，10:00 - December 5 (Fri)，17:00
Poster Presentation: December 4 (Thu)，17:45 - 18:45【Core time for odd numbers】
December 5 (Fri)， 13:00 - 14:00【Core time for even numbers】

P-001

Annual changes of the concentration of indium in ambient air in Tokyo

		 Daisuke Nakajima1, Sumio Goto2, Fujio Shiraishi1, Kazuho Inaba2, Hidetaka Takigami1
		1

National Institute for Environmental Studies, 2Azabu University

Mutagenicity of airborne particles at 3 prefectures in the coast of the Sea of Japan in winterand spring and the influence of transborder transport
		 Souleymane Coulibaly1, Tomohiro Hasei1, Nobuyuki Sera2, Tadashi Oro3, Mizuka Kido4,
Kunihiro Funasaka5, Daichi Asakawa5, Keiji Wakabayashi1, 6, Tetsushi Watanabe1
P-002

		

1

Kyoto Pharmaceutical University, 2Fukuoka Inst. Health Environ. Sci., 3Tottori Pref. Inst. Pub. Health Environ.,
Toyama Pref. Environ. Sci. Res. Center, 5Osaka City Inst. Pub. Health Environ. Sci., 6Univ. Shizuoka

4

P-003
（O-6）

Risk estimation for airborne particulate matter: comparison with smoking
Masahiko Watanabe, Hiroaki Aso, Katsuya Suemaru

P-004
（O-1）

A multidisciplinary approach to explore the etiology of esophageal cancer in China
Akane Ikeda1, 6, Mamoru Kato2, Tatsuhiro Shibata2, Isao Kurosaka3, Yingsong Lin4,
Yoshitaka Matsushima5, Osamu Endo6, Hitoshi Nakagama1, Yukari Totsuka1

		

		

P-005
（O-2）

		

P-006
（O-3）
		

School of Pharmacy, Shujitsu University

1

Natl. Cancer Ctr. Res. Inst. Div. Cancer Development System, 2Div. Cancer Genomics, 3Dept. Bioinformatics,
Aichi Med. Univ., Sch. Med., Dept. Publ. Health., 5Tokyo Univ. Agricul, 6Azabu University

4

Mutation signature analysis of occupational cholangiocarcinoma and dichrolopropane-exposed
cells.
Masanori Goto1, Sachiyo Mimaki2, Shoji Kubo3, Katsuya Tsuchihara2, Hitoshi Nakagama1,
Yukari Totsuka1
1

National Cancer Center Research Institute, 2National Cancer Center EPOC, 3Osaka City University

Germline mutation rate and its threshold in mice
Arikuni Uchimura1, Mayumi Higuchi1, Yohei Minakuchi2, Jo Nishino3, Atsushi Toyoda2, Asao Fujiyama2,
Takeshi Yagi1
1

Graduate School of Frontier Biosciences, Osaka University, 2National Institute of Genetics,
The Institute of Statistical Mathematics

3

Comparison of classification of germ cell mutagens in EU, Germany or Japan
		 Takeshi Morita, Sachiko Komiya
P-007

		

Division of Safety Information on Drug, Food and Chemicals, National Institute of Health Sciences

P-008（O-11）

From margin of exposure (MOE) toward internal MOE (iMOE) -Risk assessment ofcarcinogen using hemoglobin adductHiroshi Honda, Kenkichi Fujii, Toshio Kasamatsu, Naohiro Ikeda, Naohiro Nishiyama

		

P-009

		

		

R&D - Core Technology - Safety Science ResearchProtein Adductomics, an Emerging Field of Mutagen Exposure Monitoring
Suresh Thiruppathi1, Yoshiro Saito2, Masamitsu Honma3, Yoji Sato1, Takayoshi Suzuki1
1Div. Cellular and Gene Therapy Products, National Institute of Health Sciences,Div. Medical Safety Science, National Institute of Health Sciences,3
Div. Genetics and Mutagenesis, National Institute of Health Sciences238

P-010Single molecule detection of the DNA modification within the genome

		 Kazuhiro Shiizaki, Masanori Goto, Natsuko Hama, Yasuhito Arai, Tatsuhiro Shibata, Hitoshi Nakagama,
Yukari Totsuka
		

P-011

National Cancer Center Research instituteEstablishment of new assay for detecting inosine-containing RNA

		 Yukie Matsuda, Shigenori Iwai, Isao Kuraoka
		

P-012

Graduate School of Engineering Science, Osaka University

Analysis of DNA damage induced by aldehydes

		 Toshiaki Nakano, Ming-Zhang Xie, Mizuki Goda, Mahmoud Shoulkamy, Hiroshi Ide
		

P-013

		
		

Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University

UV-induced photohydration of cytosines in oligonucleotides
Kengo Kawano, Michio Kimura, Kazuo Negishi

Department of Pharmaceutical Sciences, Nihon Pharmaceutical University

Comprehensive analysis of DNA adducts in the liver of rats treated with non-genotoxic
carcinogen, 1,4-dioxane.
		 Shuntaro Akimoto1, 3, Mamoru Kato2, Tatsuhiro Shibata2, Osamu Endo3, Min Wei4, Hitoshi Nakagama1,
Hideki Wanibuchi4, Shoji Fukusima5, Yukari Totsuka1
P-014

		

1

Div. Cancer Dev, 2Div. Cancer Genomics, Natl. Cancer Ctr. Res. Inst, 3Azabu University,
Osaka City Uviv. Grad. Sch. Med, 5Japan Bioassay Research Center

4

Construction of site-specifically modified plasmids to detect frame-shift mutations and analysis
of TLS in human cells
		 Naohiro Kanayama1, Masanobu Kawanishi1, Takeji Takamura2, Takashi Yagi1
P-015

		

1

Graduate School of Science, Osaka Prefecture University,
Department of Applied Chemistry, Kanagawa Institute of Technology

2Analyses of TLS in human cultured cells using plasmids site-specifically modified with a single
base damage
		 Seiya Nagano1, Yuka Higashigaki1, Akira Sassa2, Masanobu Kawanishi1, Manabu Yasui2,
Takeji Takamura3, Takashi Yagi1
P-016

		

P-017
（O-14）
		1

Graduate School of Science, Osaka Pref. University,
Division of Genetics and Mutagenesis, National Institute of Health Sciences,3
Department of Applied Chemistry, Kanagawa Institute of Technology
2

Numerical genotoxic effects of clustered 8-oxoguanine DNA adducts locally introduced into
the genome
Akira Sassa, Nagisa Kamoshita, Yuki Kanemaru, Masamitsu Honma, Manabu Yasui
Division of Genetics and Mutagenesis, National Institute of Health Sciences.

Genotoxicity of perfluorooctanoic acid is due to oxidative stress		 Shuji Tsuda1, Naoto Shimizu2, Tomomi Takahashi3, Norimitsu Saito1, Yu F Sasaki3, 4
P-018

		

P-019

1

Iwate Inst Environ Health Sci, 2Agilent Technologies, 3Hachinohe Natl Co. Tech, 4Himeji Dokkyo Uni

Validation of two-complementary (Q)SAR models for bacterial mutagenicity

		 Tatsuya Kato, Shigeharu Muto, Yumiko Iwase, Yoshifumi Uno
		

P-020

Mitsubishi Tanabe Pharma Corporation

Structure activity relationship of mutageniity of poly aromatic hydrocarobons

		 Takeji Takamura1, Masahiro Ogino1, 2, Kentaro Misaki3, 4

		

1

Department of Applied Chemistry, Kanagawa Institute of Technology,
Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka,
3
Research Center of Environmental Quality Management, Kyoto University,
4
Center for Marine Environmental Studies, Ehime University
2

Construction of selection criteria of the Ames test method using predicted physicochemicalparameter values for volatile chemicals
		 Mifumi Tsubokura, Saori Fujishima, Masaru Suzuki, Shozo Ogura, Makoto Nakai
P-021

		

Chemicals Evaluation and Research Institute, Japan (CERI)

39

P-022

Assessment of mutagenic potential of the degradation products of N,N’ -diethylethylenediamine

		 Kentaro Takeshita, Chiaki Takeshita, Yoshiyuki Aratani, Toshihide Horiuchi,
Takuya Nakayama
		

P-023

Ono Pharmaceutical Co., Ltd.

Mutagenicity of ω-3 fatty acid peroxidation products in the Ames test

		 Petr Gruz1, Masatomi Shimizu1, 2, Kei-ichi Sugiyama1, Masamitsu Honma1		1Division of Genetics and Mutagenesis, National Institute of Health Sciences, 2Tokyo Healthcare University

Bacterial Mutagenicity Study Group (BMS) collaborative study by 19 research institutes:
data collection for Ames test of 20 kinds of reagents that are often used in chemical synthesis
		 Atsushi Hakura1, Takumi Awogi2, Masayuki Kato3, Kei-ichi Sugiyama4
P-024

		

1

Tsukuba Drug Safety, Eisai Co.,Ltd., 2Otsuka Pharmaceutical Co.,Ltd., 3CMIC Bioresearch Center Co.,Ltd.,
Division of Genetics and Mutagenesis, National Institute of Health Sciences

4

Effects of basic amino acids in Ames test
		 Kenichiro Suzuki, Shoji Masumori, Makoto Hayashi
P-025

		

P-026

Public Interest Incorporated Foundation Biosafety Research Center

The study of the modified Ames assay for amino acid containing material (treat & wash assay) II		 Kumiko Kawakami, Saki Negishi, Emi Masubuchi, Keita Sonohara, Hajime Sui
		

P-027

Hatano Research Institute, Food and Drug Safety Center

Further improvement of high-throughput fluctuation Ames test (Part IX)

		 Hajime Sui1, Kumiko Kawakami1, Saki Negishi1, Emi Masubuchi1, Keita Sonohara1, Masami Yamada2		1

Hatano Research Institute, Food and Drug Safety Center, 2National Institute of Health Sciences

Modified Ames test using a strain expressing human sulfotransferase on genotoxicity
evaluation of methyleugenol related compounds
		 Kazuyuki Minegawa1, Hiroshi Honda2, Yurika Fujita2, Noriko Yamaguthi1, Takayuki Fukuda1,
Yoshihiro Oguma1, Naohiro Ikeda2, Toshio Kasamatsu2, Naohiro Nishiyama2
P-028

		

P-029

1

Tokyo Laboratory, BoZo Research Center Inc., 2R&D - Core Technology - Safety Science Research

Photocytotoxicity and photogenotoxicity of fourteen selected quinolones

		 Saori Fujishima, Mifumi Tsubokura, Yu Shimamura, Shozo Ogura, Makoto Nakai
		

P-030

Chemicals Evaluation and Research Institute, Japan

Genotoxicity of animal carcinogen diphenylamine		 Toshiaki Sasaki, Chigusa Ishioka, Masumi Asakura, Shoji Fukushima
		

P-031

Japan Bioassay Research Center, Japan Industrial Safty and Health Association

Basic study of risk assessment using TK6 micronucleus test

		 Kenji Takeshita, Shota Yamamoto

UBE Scientific Analysis Laboratory, Inc

Rescue promising false positive materials from positive compounds lists of in vitro
chromosomal aberration test -Using theory of cytotoxicity index conversion		 Yurika Fujita, Hiroshi Honda, Naohiro Ikeda
P-032

		

R&D - Core Technology - Safety Science Research

Construction of DNA damage-sensing reporter assay yeasts by using HUG1 and RNR2
promoters.
		 Koki Yamamoto, Tomoya Yoshikawa, Sayoko Harashima-Ito, Masanobu Kawanishi, Takashi Yagi
P-033

		

P-034

Graduated school of Science, Osaka Prefecture University

Detection of DNA Damage Response Inhibition Using EGFP-MDC1-expressing Cells

		 Shun Matsuda1, Sin-ya Yanagisawa1, Tsuyoshi Ikura2, Tomonari Matsuda1		1Fac. of Engineering, Kyoto Univ., 2Radiation Biology Center, Kyoto Univ.

40

P-035Detection and analysis of UV-induced mutations in plant genome

		 Munehisa Nakamura1, Kozo Makino1, Tatsuo Nunoshiba2, Keiichiro Hiratsu1
		1

Department of Applied Chemistry, National Defense Academy,
College of Liberal Arts, International Christian University

2

Live cell imaging of the effect of S-phase inhibitors on cell cycle progression of mouse m5S
cells visualized with PCNA
		 Ai Kawakita, Kaori Murata, Kenji Sugimoto
P-036

		

Live Cell Imaging Institute, Graduate School of Life and Environmental Sciences, Osaka Prefecture University

Evaluation of cytotoxicity by directly measuring elongation of cell cycle of living m5S cells andapplication to micronucleus formation assay (2)		 Kenji Sugimoto1, 2, Ai Kawakita1, Kaori Murata1, 2
P-037

		

1

Live Cell Imaging Institute, 2Graduate School of Life and Environmental Sciences, Osaka Prefecture University

Establishment of novel genotoxicity evaluation systems suitable for nanomaterials based on
nanotoxicologic mechanisms.
		 Emi Fukai1, 2, Masatoshi Watanabe2, Hitoshi Nakagama1, Yukari Totsuka1
P-038

		

P-039
（O-8）
		

P-040

1

Div. Cancer Dev., Natl. Cancer Ctr. Res. Inst., 2Grad. Sch. Engineering, Yokohama Natl. Univ.

Toxic evaluation of silver nanoparticles using side scattered light in flow cytometry andphosphorylation of histone H3
Xiaoxu Zhao, Yuko Ibuki
Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka

Particle properties and the genotoxicity of titanium dioxide

		 Reimi Yoneda1, Masanobu Kawanishi1, Yukari Totsuka2, Takashi Yagi1
		1

Graduate school of Science, Osaka Prefecture University, 2National Cancer Center Research InstituteEffects of inhibition of BCRP on the chemical ligand-mediated activation of AhR in humanhepatoma cell lines.
		 Masashi Sekimoto1, Takuomi Hosaka1, 2, Kouichi Yoshinari1, Masakuni Degawa1
P-041

		

1

School of Pharmaceutical Sciences, University of Shizuoka,
Faculty of Pharmaceutical Sciences, Setsunan University

2

Evaluation of steroid hormone-like activities of river water by improved yeast-based bioassay
and HPLC analysis
		 Sayoko Harashima-Ito, Saki Nakajima, Masanobu Kawanishi, Takashi Yagi
P-042

		

Department of Biology, Graduate School of Science, Osaka Prefecture University

A liver micronucleus test in rats following dosing before and after partial hepatectomy		 Miyuki Igarashi, Mayumi Nagata, Chiharu Hattori, Satoru Itoh
P-043

		

Medicinal Safety Research Laboratories, Daiichi-Sankyo Co., Ltd.

Investigation of specimen preparation of hepatocytes in the liver micronucleus assay using
tissues fixed with formalin		 Miyuki Shigano, Rie Takashima, Hironao Takasawa, Shuichi Hamada
P-044

		

LSI Medience Corporation

Mechanism of hepatic micronuclei induction in adult rats by single-dose administration
without partial hepatectomy
		 Naho Tsuji, Soichiro Hagio, Izumi Ogawa, Masayoshi Abe, Seigo Hayashi, Yusuke Kuroda,
Yoshikazu Yamagishi, Tetsuya Yoshino, Satoshi Furukawa
P-045

		

Biological Research Laboratories, Nissan Chemical Industries, Ltd.

Influence of the storage time of cell suspension on the results of comet assay
		 Jin Tanaka, Ai Miwa, Masahito Fukumuro, Miho Nagai, Fuyumi Uno, Masakatsu Natsume,
Sawako Kasamoto, Kenichiro Suzuki, Michiyo Oba, Misako Iio, Shoji Masumori, Makoto Hayashi
P-046

		

Public Interest Incorporated Foundation Biosafety Research Center

41

Comparison of the level of DNA damage between RccHan:WIST rats and CD(SD) rats
- negative and positive control data 		 Sawako Kasamoto, Masahito Fukumuro, Ai Miwa, Jin Tanaka, Misako Iio, Masakatsu Natsume,
Kenichiro Suzuki, Fuyumi Uno, Shoji Masumori, Makoto Hayashi
P-047

		

Public Interest Incorporated Foundation Biosafety Research Center

P-048（O-10）

Integrated analysis of microRNA expression profile and Comet assay in the mouse liver forcorrect classification of genotoxic compounds
Hiroyuki Oka1, Takeki Uehara2, Yuji Morikawa1, Hiroyuki Hanafusa1, Koichi Masuno1,
Hirofumi Miyajima1

		

P-049

1

Research Laboratory for Development, Shionogi & Co., Ltd.,
Global Project Management Department, Shionogi & Co., Ltd.

2

Genotoxicity of acrylamide in germ cells of gpt delta mice		 Soichiro Hagio1, Izumi Ogawa1, Masayoshi Abe1, Seigo Hayashi1, Naho Tsuji1, Yusuke Kuroda1,
Satoshi Furukawa1, Takashi Yagi2, Masamitsu Honma3, Kenichi Masumura3
		1

Biological Research Laboratories, Nissan Chemical Industries, Ltd.,
Graduate School of Science, Osaka Prefecture University,
3
Division of Genetics and Mutagenesis, National Institute of Health Sciences
2

Integration of micronucleus test (peripheral blood, bone marrow, liver, colon) into genemutation assay of F344 gpt delta transgenic rat using dimethylhydrazine
		 Hisako Hori1, Yasuhiro Tanaka1, Eri Tsutsumi1, Ayaka Momonami1, Kenichi Masumura2,
Masami Yamada2, Wataru Fujii1, Yoshinori Kitagawa1
P-050

		

P-051

1

Suntory Business Expert Limited, 2Division of Genetics and Mutagenesis, National Institute of Health Sciences

Effects of a high-fat diet on in vivo mutagenicity induced by heterocyclic amine in the colon of
gpt delta rats

		 Shinji Takasu1, Yuji Ishii1, Aki Kijima1, Yuh Yokoo1, Takehiko Nohmi2, Akiyoshi Nishikawa2,
Kumiko Ogawa1, Takashi Umemura1

		1Division of Pathology, National Institute of Health Sciences,
Biological Safety Research Center, National Institute of Health Sciences2

Age-dependent alterations in mutagenesis induced in lungs of gpt delta mice by intratracheal
administration of benzo[a]pyrene
		 Yasunobu Aoki1, Akiko H Hashimoto1, Yoshiki Sugawara1, Kyoko Arai-Hiyoshi1, Sataro Goto2,
Kenichi Masumura3, Takehiko Nohmi3
P-052

		

P-053

1

Center for Environmental Risk Research, National Institute for Environmental Studies,
Graduate School of Health and Sports Science, Juntendo University,
3
Division of Genetics and Mutagenesis, National Institute of Health Sciences
2

Interlaboratory Trial of the Rat Pig-a and PIGRET Assays: MMS collaborative study

		 Masami Yamada1, Katsuyoshi Horibata1, Akiko Ukai1, Takafumi Kimoto2, Satsuki Chikura2,
Satoru Itoh3, Shigeharu Muto4, Yoshifumi Uno4, Hisakazu Sanada5, Rie Takashima6, Miyuki Shigano6,
Hironao Takasawa6, Shuichi Hamada6, Mika Yamamoto7, Hisako Hori8, Eri Tsutsumi8, Kunio Wada9,
Akihisa Maeda10, Mizuki Kosaka11, Aoi Kimura11, Ryuta Kikuzuki12, Yousuke Ogiwara12,
Takahiro Kyoya13, Hideki Adachi14, Yasuaki Uematsu14, Ikuma Yoshida15, Kazunori Narumi16,
Takayuki Fukuda17, Yuta Suzuki17, Ken Goto17, Takeshi Morita1, Masamitsu Honma1
		

P-054

1

Division of Genetics and Mutagenesis, National Institute of Health Sciences, 2Teijin Ph., 3Daiichi Sankyo,
Mitsubishi Tanabe Ph., 5KAKEN PH., 6LSI Medience, 7Astellas Ph., 8Suntory B.E., 9The Inst. Env. Tox.,
10
Toray, 11SNBL, 12Taisho Ph., 13Kumiai Chemical Ind., 14Dainippon Sumitomo Ph., 15Takeda.,
16
YAKULT HONSHA, 17BoZo R.C.
4

MMS/Pig-a collaborative study: Evaluation of the genotoxicity of Acrylamide

		 Katsuyoshi Horibata, Akiko Ukai, Masamitsu Honma
		

P-055

Division of Genetics and Mutagenesis, National Institute of Health Sciences

The Collaborative Study of Rat Pig-a /PIGRET Assay: Diethylnitrosamine

		 Kunio Wada, Yuzo Takezawa, Misaki Abe, Kyomu Matsumoto		

The Institute of Environmental Toxicology, Toxicology Division42

P-056Detection of in vivo mutagenesis induced by EMS and iPMS with Pig-a and PIGRET assays

		 Satoru Itoh, Chiharu Hattori, Akiharu Hanamoto, Shiho Nakayama
		

P-057

		
		

P-058
（O-9）
		

P-059

Medicinal Safety Research Labratories, Daiichi Sankyo Co., Ltd.

Pig-a gene mutation and micronucleus induction in rat peripheral blood by methyl
methanesulfonate
Shigeharu Muto, Katsuya Yamada, Tatsuya Kato, Yumiko Iwase, Yoshifumi Uno
Mitsubishi Tanabe Pharma Corporation

Induction of mouse colon tumors by colon–mutagenic noncarcinogens in a colitis model
Naoki Koyama1, Yuki Seki1, Jiro Sonoda1, Shinya Tanahashi2, Satoru Hosokawa1, Shoji Asakura1,
Kyoko Nakano1, Atsushi Hakura1
1

Tsukuba Drug Safety, Eisai Co., Ltd., 2Preclinical Safety Research Laboratories, Sunplanet Co.,Ltd

Identification of genotoxic mode of action of alectinib

		 Kaori Matsuzaki, Shigeki Motoyama, Kenji Tanaka, Asako Harada, Junko Taketo, Chiaki Katoh,
Akira Takeiri, Masayuki Mishima		Chugai pharmaceutical Co., Ltd., Research Division

Relation between micronucleus induction by N -ethyl-N -nitrosourea and circadian rhythm oferythropoietin secretion
		 Keiichi Ito1, 2, Shoji Masumori2, Daisuke Mukai2, Makoto Hayashi2, Hiroyuki Sakakibara3,
Michiko Yasuda1, Kayoko Shimoi1
P-060

		

P-061

1

Grad. Sch. of Integra. Pharma.and Nutr. Sci. Univ. of Shizuoka,
Public Interest Incorporated Foundation Biosafety Research Center, 3Dept. of Agricul. Univ. of Miyazaki

2

Possible role of Nrf2 in the in vivo mutagenicity of Nitrofurantoin

		 Aki Kijima1, Yuji Ishii1, Shinji Takasu1, Yuh Yokoo1, Takuma Tsuchiya1, Yukio Kodama2,
Takashi Umemura1

		1Division of Pathology,National Institute of Health Sciences,
Division of Toxicology,National Institute of Health Sciences

2

Involvement of cell proliferation and PP2A inactivation in gene mutation by thehepatocarcinogen estragole
		 Yuji Ishii1, Shinji Takasu1, Aki Kijima1, Yuh Yokoo1, Takuma Tsuchiya1, Yukio Kodama2,
Kumiko Ogawa1, Takashi Umemura1
P-062

		

P-063

1

Division of Pathology, National Institute of Health Sciences,
Biological Safety Research Center, National Institute of Health Sciences2

The influence of Glutathione deficiency on DNA oxidative damage elicited by X-ray radiation

		 Yun-Shan Li1, Yuya Kawasaki1, Hiroshi Kasai1, 2, Kazuaki Kawai1
		

P-064

1

Department of Environmental Oncology, Occupational and Environmental Health University, 2OHG Institute

Clinical research for antigenotoxicity of Connarus extract against smoking

		 Yu F Sasaki1, 2, Takanori Nakamura2, Ryo Murashige2, Taizou Taniguchi2, Kayoko Yamasaki2,
Gisho Honda2, Kasumi Ainai1, Yumi Ishida1, Yoshihisa Kitamura3, Kazuo Mitani4

		

P-065

1

Hachinohe National College of Technology, 2Himeji Dokkyo University, 3Kouiken Co. Ltd., 4Mitani Family Clinic

Isolation and identification of anti-mutagens in green tea against 1-nitropyrene

		 Tomohiro Hasei, Megumi Fujihashi, Fumitaka Shinkai, Yuki Kawai, Tetsushi Watanabe
		

P-066

Kyoto Pharmaceutical University

Antimutagenicity of A. arguta and its mechanisms

		 Misako Tada1, Naoko Miyake1, Yuma Okimasu2, Mari Nishimura2, Tomoe Negishi2,
Sakae Arimoto-Kobayashi2
		1

Faculty of Pharmaceutical Sciences, 2Dept. Med. Den. Pharm. Sci.

Modifying effects of Actinidia arguta and its components on 1,2-dimethyl- hydrazine inducedcolon preneoplastic lesions in male F344 rats
		 Naoko Miyake1, Misako Tada1, Yuma Okimasu2, Mari Nishimura2, Chiharu Asahi2, Ryoko Hida2,
Tomoe Negishi2, Sakae Arimoto-Kobayashi1, 2
P-067

		

1

Fac. Pharm. Sci., 2Grad. Sch. Med. Den. Pharm. Sci., Okayama University43P-068Antimutagenic mechanism of soybean extract on N -methyl-N -nitrosourea

		 Seiya Aizawa1, Keiko Inami1, Jin Tatsuzaki2, Masataka Mochizuki1
		1Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2Tokiwa Phytochemical Co., LTD

The Genomedefender activities of Aomori prefecture blackcurrant, In vitro and in vivo
evaluation.
		 Ayumi Yamamoto1, Tokuhisa Hirouchi2, Kana Nakashima1, Saori Kawamorita1, Yugo Okuya1,
Yoshihiro Kamitai3, Yoji Kato4
P-069

		

P-070

		

		

P-071
（O-15）
		

P-072

1

Faculty of Chemical and Biological Engineering, Hachinohe National College of Technology,
Department of Radiobiology, Institute for Environmental Sciences, 3Center for Joint Research, Hirosaki University,
4
aculty of Education, Hirosaki University
2

Enzyme Kinetics of an Alternative Splicing Isoform of Mitochondrial 8-Oxoguanine DNA
Glycosylase, OGG1-1b, and compared with the nuclear OGG1-1a
Akira Ogawa1, Takashi Watanabe1, Sayaka Shoji1, Chie Furihata1, 2
1

Aoyama Gakuin University, 2Present adress: National Institute of Health Sciences

Oxidative stress-induced intestinal tumors in Mutyh deficient mice

Mizuki Ohno1, Noriko Takano1, Fumiko Sasaki1, Kenichi Taguchi2, Yusaku Nakabeppu3, 4,
Yasunobu Aoki5, Takehiko Nohmi6, Yoshimichi Nakatsu1, Teruhisa Tsuzuki1
1

Dept. of Medical Biophysics and Radiation Biology, Faculty of Medical Sciences, Kyushu University,
Cancer Pathology Lab., National Hospital Organization Kyushu Cancer Center,
3
Division of Neurofunctional Genomics, Medical Institute of Bioregulation, Kyushu University,
4
Research Center for Nucleotide Pool, Kyushu University,
5
Center for Environmental Risk Research, National Institute for Environmental Studies,
6
Division of Genetics and Mutagenesis, National Institute of Health Sciences2

Oxidative DNA damage-induced mutagenesis in Mutyh -deficient mice

		 Noriko Takano1, Mizuki Ohno1, Yohei Inaba2, Tsutomu Shimura2, Naoki Kunugita2, Yusaku Nakabeppu3, 4,
Yoshimichi Nakatsu1, Teruhisa Tsuzuki1
		

P-073
（O-12）

		

1

Department of Medical Biophysics and Radiation Biology, Faculty of Medical Science, Kyushu University,
Department of Environmental Health, National Institute of Public Health,3
Division of Neurofunctional Genomics, Medical Institute of Bioregulation, Kyushu University,
4
Research Center for Nucleotide Pool, Kyushu University2

Formaldehyde delays the repair of pyrimidine dimers and enhances the sensitivity to UVGuang Yang, Yuko Ibuki
Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka

Long-wavelength UVA1 suppresses the repair of DNA damage induced by short-wavelength
UV		 Yuko Ibuki, Guang Yang
P-074

		

Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka

An in vivo protective roles of DNA polymerase kappa against induction of DNA double strand
breaks by mitomycin C － Immunohistological analysis with γH2AX as an index －		 Shigeki Motoyama1, Akira Takeiri1, Saori Matsuo1, Naoko Wada2, Kouichi Jishage2, Masayuki Mishima1,
Naoko Niimi3, Petr Gruz3, Kenichi Masumura3, Masami Yamada3, Takehiko Nohmi4
P-075

		

1

Chugai Pharmaceutical Co., LTD.,Research Division, 2Chugai Research Institute For Medical Science, Inc.,
Div. Genetics & Mutagenesis, NIHS, 4Biological Safety Research Center, NIHS

3

Purification and identification of pyrophosphatase Ham1 for the sanitization of deaminatednucleotides in Thermus thermophilus
		 Miki Nishimura1, Emiko Morimoto2, Keiichiro Hiratsu3, Tatsuo Nunoshiba1, 2
P-076

		

P-077

1

Graduate School of Arts and Sciences, International Christian University,
College of Liberal Arts, International Christian University,
3
Department of Applied Chemistry, National Defense Academy
2Analysis of Genomic Integrity in Thermus thermophilus -The Roles of AP endonuclease-

		 Reiko Aihara1, Hanaka Mera2, Kazune Ezaki2, Keiichiro Hiratsu3, Tatsuo Nunoshiba1, 2
		1Graduate School of Arts and Scheinces, ICU, 2College of Liberal Arts, International Christian University,
Department of Applied Chemistry, National Defense Academy

344

P-078Human EEPD1 has nuclease activity

		 Kenji Okada, Shigenori Iwai, Isao Kuraoka
		

P-079

Graduate School of Engineering Science, Osaka University

Re-establishment of in vitro Nucleotide excision repair assay using human cell extract

		 Yuina Sonohara, Shigenori Iwai, Isao Kuraoka
		

P-080

Graduate School of Engineering Science, Osaka University

Establishment of TLS polymerase mutants in Medaka using TALENs

		 Yoshihiro Fujikawa1, Tomoko Fujiwara1, Tetsushi Sakuma2, Takashi Yamamoto2, Takeshi Todo1
		

P-081

1

Graduate School of Medicine, Osaka University, 2Graduate school of science, Hiroshima University

Translesion synthesis past an oxidatively damaged guanine by REV1 and DNA polymerase ζ.

		 Masayo Suzuki1, 2, Taishu Kawada1, Masayuki Morikawa1, 2, Takanobu Kobayashi1, Hiroshi Miyazawa1,
Katsuhito Kino1
		1

Laboratory of Molecular Biology, Kagawa School of Pharmaceutical Sciences, Tokushima Bunri,
Research Fellow of Japan Society for the Promotion of Science2

Analysis of types of DNA damage by tirapazamine using various DNA repair deficient DT40
cell lines.
		 Takahito Moriwaki1, Saki Okamoto1, Hiroyuki Sasanuma2, Hideko Nagasawa3, Shunichi Takeda2,
Shinichiro Masunaga1, Keizo Tano1
P-082

		

1

Research Reactor Institute, Kyoto University, 2Graduate school of Medicine, Kyoto University,
Graduate school of Pharmaceutical, Gifu Pharmaceutical University

3

The Werner syndrome protein promotes mutagenesis induced by 8-hydroxyguanine pairedwith adenine
		 Hiroyuki Kamiya1, 2, Eri Nakamura2, Tetsuaki Makino1, 2, Miwako Kobayashi3, Ichiro Matsuoka3
P-083

		

1

Grad. Sch. Biomed. Hlth. Sci., Hiroshima Univ., 2Grad. Sch. Sci. Engng., Ehime Univ.,
College Pharm. Sci., Matsuyama Univ.

3

Detection of 5-aza-2’-deoxycytidine using yeast transformants containing human DNMT genes
		 Kei-ichi Sugiyama, Makiko Takamune, Hiroko Furusawa, Masamitsu Honma
P-084

		

P-085

Div. Genetics and Mutagenesis, Natl. Inst. Health Sci., Japan

Detection of DNA demethylating agents by using MGMT inactivated cells

		 Miyuki Taniguchi, Masanobu Kawanishi, Takashi Yagi
		

P-086

Graduate School of Science, Osaka Pref. Univ.

Epigenetic changes in the H-19 gene of cows produced by somatic cell nuclear transfer

		 Shizuyo Sutou, Hiroko Morita, Toshiyuki Kudo
		

School of Pharmacy, Shujitsu University

45