WAKATE INITIATIVE SEMINAR
演題: A NEW METHOD OF ENGINEERING INTERFACES FOR BIO-MOLECULE SURFACEIMMOBILISATION.
演者：PROF. MARCELA BILEKSCHOOL OF PHYSICS, A28, UNIVERSITY OF SYDNEY, NSW, 2006, AUSTRALIA,
日時：2010 年 6 月 9 日（すいよび） 12:00 – 1:00pm
会場：医学系学系棟 272（Igakukei-gakukeitou room 272）

要旨：The ability to strongly attach biomolecules such as proteins including enzymes and antibodies to surfaces underpinsa host of technologies which are rapidly growing in utility and importance. Such technologies include biosensors for
medical and environmental applications and protein or antibody diagnostic arrays for early disease detection. Emerging
new applications include continuous flow reactors for enzymatic chemical, textile or biofuels processing and“proactive” implantable biomaterials[1,2] which interact with their host via an interfacial layer of active biomolecules
to direct a specific desired response to the implant. In many of these applications it is desirable to maintain physicalproperties (including mechanical and electrical) of an underlying material whilst engineering a surface suitable forattachment of proteins with high levels of bioactivity. Nanoscale integrated polymeric interlayers are therefore attractive
for this purpose.
Over the last few years we have developed a new biomolecule binding interlayer with significant advantages over othercurrently available methods of achieving protein attachment to surfaces[3]. Our technology, based on the implantationof ions[4,5] from a plasma, achieves covalent attachment of biomolecules to surfaces. This attachment occurs
spontaneously by immersion of the surface in protein containing solution, without the use of chemical linkers, and assuch avoids the complex wet chemistry typically required to achieve covalent attachment and the requirements for theunderlying surface to present particular termination groups. Our method can be used on any underlying material. The
covalent attachment is achieved through the action of highly reactive free radicals in the interlayer which couple toproximate side chains of the protein. The fact that a high proportion of side chains groups participate in the formation ofcovalent bonds with the radicals means that the covalent binding capacity of the interlayer is effective with virtually all
biomolecules. Because physical adsorption is not the mechanism of protein adhesion, the chemical and physicalproperties of the interlayer can be tuned so as to maximise the bioactivity of the bound proteins.
In this paper we present characterisation of the structure and properties of such protein binding interlayers and describe
a detailed model for the covalent attachment of protein molecules from solution. The prospects for utilising theseinterlayers to functionalise the surfaces of conducting polymers and metals for applications as electrodes for cellstimulation will be discussed. The surface modification of self-assembled block copolymers using our techniques also
opens up the possibility of creating controlled nanopatterns of functional protein molecules for basic research on
isolated proteins or the inclusion of isolated biomolecules in mass produced nanoscale devices.
homepage: http://www.physics.usyd.edu.au/research/applied/research/platform.shtml

References:[1] Bax DV, McKenzie DR, Weiss AS, Bilek MMM, Biomaterials, 31: 2526-2534 (2010)[2] Bax DV, McKenzie DR, Weiss AS, Bilek MMM, Acta Biomaterialia, 5(9) 3371-3381 (2009)[3] Bilek M, McKenzie DR, “Plasma modified surfaces for covalent immobilization of functional biomolecules in theabsence of chemical linkers: towards better biosenors and a new generation of medical implants”, Biophysical Reviews,(in press DOI 10.1007/s12551-010-0028-1) (2010).
[4] M. Bilek, D.R. McKenzie, R.C. Powles, “Treatment of polymeric biomaterials by ion implantation” in Biomaterials
and Surface Modification (ed. P.K. Chu and X. Liu)”, Research Signpost 2007.
[5] A. Kondyurin and M. Bilek, Ion Beam Treatment of Polymers: Application Aspects from Medicine to Space,Elsevier (Oxford), 2008.

連絡先：若手イニシアティブ
Damien Hall TEL: 029-853-8008
Email: damienhall@md.tsukuba.ac.jp