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PSIM Professional Version 9.3.400 X32.rar Full form of the vector, degree of expression, area of gene expression (that is, the more surface area of cells expressing a given transgene, the greater the effect), levels of gene expression, and the like. The dosage and timing of expression of the transgene can be established by transfection of the gene into the target cells so that the gene is integrated in thegenome of the cells and replicated with the host genome. This integration and expression can be achieved by any of a variety of techniques. One such technique is, for example, the use of a retroviral expression system in which the therapeutic polynucleotide of the invention is initially cloned into a retroviral expression vector such as pMX-insulin.sup.1, which is subsequently transfected into apackaging cell line. The packaging cell line, which has the additional advantage of constitutively secreting the retroviral proteins necessary for production of infectious virus, produces viral stock after multiple passages. The retroviral-produced infectious virus is used to infect target cells, for example lymphocytes. A retroviral expression system is preferable to cell-based transfection of thepolyisomcyin polymers since it provides for integration of the therapeutic polynucleotide in the target cells, thus resulting in stable expression. The expression of the therapeutic polynucleotide is easily controlled by the person of ordinary skill in the art by varying the amount of the therapeutic polynucleotide provided in the viral stock. Once a cell is transfected with the retroviral polynucleotide of theinvention, that cell may be further genetically modified to produce the polyisomcyin polymers. This may be accomplished by addition of various exogenous DNA to the target cells. Specific techniques for accomplishing these modifications are described in detail herein. Once the preferred form of the invention has been developed, it will be recognized by those skilled in the art as suited for
application to other polymers, such as human polyisomcyin, by routine experimentation. In accordance with another embodiment of this invention, polyisomcyin is covalently attached to an agent which is capable of specifically delivering the polyisomcyin to various sites within the body in order to enhance the therapeutic effect. This can be accomplished by techniques known in the art, such as
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