Compatibility of Soluge with other materialsHighmolecularweightand changes in the appearance were observed.
Finalconcentration

Compatibilitywith 0.1% Soluge

Sodium hyaluronate (high molecular weight)

0.25%

○

Sodium hyaluronate (low molecular weight)

0.25%

○

Xanthan gum

0.25%

○

Sodium alginate

0.25%

○

Carbomer 1 (Carbopol 940)

0.25%

○

Carbomer 2 (Carbopol AQUA SF-1)

1.5%

○

While precipitates or turbidities appeared in the soluble collagen, noturbidities appeared in Soluge.

Carbomer

Sodium hyaluronate

and allowed to stand at 23℃. Each mixture was stored for four weeks

Ingredient

SolugeFourth-Generation Collagen

Soluge was mixed with each of the following cosmetic raw materials

Lowmolecularweight

Xanthangum

Sodiumalginate

12(Atelocollagen)SolugeSolublecollagenFrom in-house data

pH Stability
AcidNeutralAlkaline

The pH stability of Soluge avoids the solubility disadvantages

Soluge◎◎○*of water-soluble collagen, making it useful for a wide variety

Soluble collagen○××of formulations.

*Turbidity appeared at around pH 9.5.

From in-house data

Firmness andelasticity
Prevention ofsagging
Promotes collagen gel
contraction

Recommended amount for blending: 0.5%‒1%Anti-Wrinkle
AFN-221

Soluge 1% PE

ATELOCOLLAGENಓؑ࢒ჷWater/Phenoxyethanol

1 kg

The country of origin: Japan The Place of origin: Miyagi Pref.

Commitment
Focus on raw materials

Domestic sourced raw material

Eﬀective use of resources(Sustainability)

Clean formulation(Buﬀer free)

We selected skin from the upper

Atelocollagen is an eco-friendly

We simpliﬁed the ingredients oflobe of the blue shark due to its

cosmetic raw material made byatelocollagen without adding ahigh purity. The raw material

eﬀectively using an inedible part

buﬀer, which had conventionallyoriginates from Kesennuma,

that is diﬃcult to process.

been added to maintain stability.

Miyagi Prefecture.

© 2020 KOKEN CO., LTD. C-1-3005-1-01-00

Promotes synthesis of type I/IIIcollagen and elastin

Various shades(gradation) ofmoisturizing
Provides double the moistureof hyaluronic acidSoluge

Fourth-Generation Collagen(Atelocollagen)History of industrial use of collagen

Soluge is a middle size collagen produced by harnessing technology to extract atelocollagen,a type of collagen used for medical purposes. It is a fourth-generation collagen based on thenew concept of displaying the low molecular weight of hydrolyzed collagen and the highmolecular weight of soluble collagen. We provide the high-quality, rare atelocollagen raw
material.

Firmness andelasticity
Prevention ofsagging

Promotes collagen gel
contraction

Anti-Wrinkle

Water-retaining capability

Moisturizing capabilityFirst
generation
Secondgeneration

Gelatin (1700s)

Hydrolyzed collagen (Soluge) was dripped onto a piece of ﬁlter

After applying each sample to the test site for one minute, the sample

Peptide-like collagen (1930s)

paper. Five minutes later, the collagen in weight was determined inwas wiped away and the change in electrical conductivity was

Third
generation

Atelocollagen (1960s)

order to calculate the moisture retention ratio. Soluge has higher

determined in order to calculate the change in moisture content ofmoisture retentivity than hydrolyzed collagen.

skin corneum. Soluge showed double the moisturizing capability of

Fourthgeneration

Middle size moleculecollagen (Soluge)

Ultra-low-molecular/modiﬁed collagen era

sodium hyaluronate (the molecular weight of which is approximately100,000).
Waterretention
ratio after ﬁve
minutes (%)

Promotes synthesis oftype I/III collagen andelastin

Provides double themoisture ofhyaluronic acid

Various shades(gradation) of
moisturizing

Change inmoisture contentof skin corneum
4 min after thesample waswiped away
1%hydrolyzed
collagen

Water

1%
SolugeFrom in-house data

0.01% sodium
hyaluronate

Moisturizing illustration
Koken's unique middle-sizing method
We have successfully developed this fourth-generation collagen with various shades (gradation) using Kokenʼs unique additive-free production
method without chemical treatment.
Extraction process

Telopeptide-free
production method(also employed formedical use)

Raw material (such as skin)C-telopeptide

SolugeProduction method
containing telopeptides

Wide range of molecular weight

Enzymatic
degradation

GelatinPuriﬁcation

・High moisturizing capability・Size gradation

Production methodwith chemicaltreatment

Cause of allergies

Amino acid composition
Soluge, whose amino acid composition is similar to human skinweight ranging from high to low, features characteristics of bothcollagen, is enriched with proline and hydroxyproline, featuring ahydrolyzed collagen and soluble collagen.

high aﬃnity with the skin and excellent moisture retention.
Compared with other collagen derived from bovine, porcine, andtuna. Soluge is enriched with basic amino acids that are readilyHigh molecular weightcollagen(soluble collagen)Molecular
weightHigh

SolugeLow molecular weightcollagen(hydrolyzed collagen)1‒100,000

Several hundreds
to tens of thousands

Type I collagen (human)

Molecular
weight300,000- High moisturizing
eﬀect- Coats and protects
the skin
Advantages - Immediately feelthe eﬀects(moisturizing/ﬁrming)
Disadvantages

Low

absorbed into the skin and hair (mainly arginine).

ImpermeableNewtheory

Soluge moisturizes the skininside and out

Hydrolyzed collagen

Soluge, which contains collagen with a wide range of molecularHighmolecular
collagen Soluge

Collagen peptide(Hydrolyzed collagen)

Unique chemicaltreatment-free
production method

Puriﬁcation

Heat extraction

Site of allergic reaction

Solugemoisturizes the skin inside and out.

Pretreatment

Triple-helix region

N-telopeptide

Atelocollagen

Triple-helical collagenmoisturizing function. Collagen in a wide range of sizes incrementallySoluge

Low-molecularization Puriﬁcation
treatment

Puriﬁcation

moisturizing the skinʼ s surface. Soluge features a gradational

High-molecular weight collagen(Soluble collagen)Low-molecularization process

Enzymatic treatment

0.01% Soluge(containing 1%)
From in-house data

While conventional high-molecular weight collagen focuses on

Soluge is a high-quality middle size molecule collagen originating from atelocollagen without telopeptides, which can trigger allergic reactions.

DoubleGradational moistureretention

Approach to cells■ Firmness/Elasticity/Prevention of sagging■ Anti-wrinkle

Reduced contractility of the dermal extracellular matrix is one cause

Promotes the synthesis of dermal matrix components that give skinof declining ﬁrmness/elasticity and sagging related to aging. The

its elasticity (type I collagen, type III collagen, elastin), making thecollagen gel contraction that simulated the dermis demonstratedskin elastic and ﬁrm.

that Soluge promotes gel contraction.

[Promoting synthesis of dermal matrix components]
Soluge and hydrolyzed collagen were added to ﬁbroblastic cells, and[Collagen gel contraction test]
Soluge was added to collagen gel mixed with ﬁbroblastic cells, andthe cells were cultured for seven days. Then the gel size wasmeasured.

the cells were cultured for 72 hours. Real-time PCR was used todetermine the gene expression levels of type I collagen, type III collagen,and elastin.

SolugeHow the gel contracted(treated with Soluge)- High permeability- High aﬃnity withcells- Activates cells

Gel size measurement

Collagen gel contracted
approximately 30%
in the group treatedwith Soluge*The ratio calculatedcompared with theuntreated control
group on Day 7mRNA
expression ratio(VS GAPDH)

Type I collagenType III collagen

Elastin

1.4x

1.8x

1.8x

Eﬀects take longer
to feelSoluge enables you to feel the beneﬁts of both
high- and low- molecular collagen.

Glycine

AlanineBasic amino acids

ProlineHydroxyproline Acidic

Acidic amino acids

OthersFrom in-house data

Nothing
added

0.005% Soluge(containing 0.5%)
From in-house data

Nothing added

0.1% hydrolyzed collagen (containing 10%)

0.1% Soluge (containing 10%)
From in-house data