092_12_KM EMT Pathway Card:A4 02/05/2012 14:49 Page 1

Epithelial-to-Mesenchymal Transition (EMT)in heart development
E 8.5

E 9.5

E 9.5

OFTEMTAtrium
AVC

AVC
development

Looping processof the linear
heart tube

Ventricle

E1
13.5
3.5 - aadultdul
OFT

Outflow tractcushions

42

in AVC andd OFT
OF
Endocardialcushion formation

RAValve formation
RV

AV cushions
LV

31(1) TTricuspid and(3) Mitral valve derived
from AVC cushion

LARVLV(2) Pulmonary and (4) aorticvalve derived from OFT cushion

Atrioventricularcanal (AVC)TGF !2

NFAT2

NFAT2

SNAIL/SLUG

SNAIL/SLUG
MMP15Notch 1
Hey1/2

MMP15

Notch 1
EMTtransitionKeyEMT
transition

BMP2: Endocardium: Cardiac jelly

BMP2Outflow tract(OFT)Neural crest cells: Myocardium
BMP2

Epithelial-to-mesenchymal transition (EMT) is a process necessary for formation of 1) the mitraland tricuspid valves in the atrioventricular canal (AVC) and 2) the aortic and pulmonary valves in
the outflow tract (OFT) region during development of the heart. An EMT is a biological process thatallows a polarized epithelial cell to undergo multiple biochemical changes to become amesenchymal cell that can migrate away from the epithelial layer in which it originated.
In the endocardium of AVC, Notch1 suppresses BMP2 activity through HEY1/2 activation while itpromotes non-invasive EMT through activation of TGFβ2 and SNAIL (SNAI1). BMP2, secreted

Discover more at abcam.com/emt
Produced in collaboration with Dr. Kristina BuacJagged/Notch1

from the adjacent myocardium, is necessary to trigger a complete invasion of endocardial cells byinducing SNAIL/SLUG (SNAI2) activity in conjunction with Notch1. SNAIL directly interacts withMMP15 to induce mesenchymal phenotype in the endocardial cells. NFAT2 (NFATC1) acts in acell-autonomous manner to suppress SNAIL/SLUG activity and inhibit EMT.
In the myocardium of OFT, Jagged1/Notch1 signaling stimulates FGF8 and BMP4 signaling. BMP4,in turn, signals to the endocardium to initiate EMT by stabilizing SNAIL/SLUG and by promoting
neural crest cell differentiation, which will further contribute to OFT remodeling and septation.

FGF8BMP4References:
1. Pompa et al., Dev Cell 22(2):244-54 (2012)
2. MacGrogan et al., Birth Defects Research (Part A): Clinical and Molecular Teratology
91:449-459 (2011)
3. Wu et al., Cir. Research 109:183-192 (2011)
4. Tao et al., Dev Bio 359(2):209-221 (2011)

092_12_KM EMT Pathway Card:A4 02/05/2012 14:49 Page 2

Epithelial to Mesenchymal Transition (EMT) related products from Abcam

Clonality

Applications

Host

Cross Reactivity

Product code

BMP2

P

WB, ICC/IF

Rb

Ms, Huab82511

BMP2P

Dot Blot, ELISA, WB, ICC/IF, IHC-Fr, IHC-P

Rb

Ms, Rat, Huab14933

BMP4

P

WB, IHC-P, ICC/IF

Rb

Ms, Rat, Hu

ab39973

FGF8P

WB, IHC-P

Rb

Ms, Rat, Hu

ab81384

HEY1

PWB, IHC-P

Rb

Ms, Huab22614HEY2PWB

Rb

Ms

ab25404

Jagged1P

WB, ICC/IF

Rb

Hu

ab85763

MMP15

M

IHC-P, WB

M

Hu

ab56308

NFAT2

M

ICC, ChIP, WB, IHC-FoFr, IP, Gel supershift assays, IF, IHC-P

M

Ms, Rat, Hu, Mk

ab2796NFAT2P

IHC-P, WB

Rb

Ms, Rat, Chk, Hu, Chmp

ab25916

Nkx2.5

PWB, IHC-P

RbMs, Hu

ab35842

Notch1 (activated)P

IHC-P, IHC-Fr, ICC/IF, WB, Flow Cyt

RbMs, Hu

ab8925

Notch1P

WB, ChIP, ICC/IF, IHC-Fr, IHC-P

Rb

Ms, Huab27526

NOTCH3P

WB, IHC-P, ICC/IF, IHC-FoFr, IHC-Fr

Rb

Ms, Rat, Hu

ab23426

NOTCH4PWB, ICC

RbHuab33163

SNAIL + SLUGP

Sandwich ELISA, WB, ICC/IF, IHC-P, ChIP, IP

Rb

Ms, Rat, Hu

ab85931

TGF beta 2M

IHC-P, WB, IHC-Fr

M

Ms, Cow, Hu

ab36495

TGF beta 2P

WB, IP, ELISA, IHC-P, ICC/IFRb

Ms, Rat, Hu

ab66045

ProteinsHostSize

Product code

BMP2 (Hu)E. coli

50μgab87065

BMP4 (Hu)E. coli

10μgab51998

FGF8 (Hu)E. coli

25μg

ab50128

NFAT2 (Hu)

Sf950μgab64307

Notch1 (Hu)wheat germ

10μg

ab114178

Notch3 (Hu)wheat germ

10μg

ab114234

TGB beta 2 (active) (Hu)HEK 2935μg

ab84070

Kits

Tests

BMP2 Human ELISA Kit

96

ab119581

BMP2 Human ELISA Kit

96

ab119582

BMP4 Human ELISA Kit

96ab99982

TGF beta 2 Human ELISA Kit

96ab100648

Product code

Featured antibodies
Anti-Calponin antibody (ab700)
Clonality
M

Applications
IHC-Fr, Flow Cyt, ICC/IF,IHC-FoFr, IHC-P

HostM

Species cross reactivity
Rat, Hu

Anti-Cardiac Troponin I antibody (ab47003)
Clonality
P

Applications
Host
WB, ICC/IF, IHC-P, Flow Cyt Rb

Species cross reactivity
Ms, Rat, Hu, Pig

Anti-CD31 antibody (ab28364)
Clonality
P

Applications
IHC-Fr, IHC-P, ICC/IF,IHC-FrFl, WB

HostRbSpecies cross reactivity
Ms, Hu

HostRbSpecies cross reactivity
Ms, HuAnti-Nkx2.5 antibody (ab35842)
Clonality
P

Applications
WB, IHC-P

Anti-SM22 alpha antibody (ab14106)
Clonality
P

Applications
ICC/IF, IHC-P, IHC-Fr,WB, ICC

HostRbSpecies cross reactivity
Ms, Rat, Chk, Cow,
Hu, Pig
092_12_KM

Antibodies

Discover more at abcam.com/emt
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