Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)
This database lists cell lines that are known to be cross-contaminated or otherwise misidentified.

The list acts as a preliminary guide to avoiding suspect cell lines; check each cell line that you use before you start work, to see if others have shown it to be problematic. You should also test all cell lines (e.g., by STR profiling for humancell lines) and compare with the reference stock profile before use.
Cell lines are listed in alphabetical order. Table 1 contains those cell lines where there is no authentic stock known to the list contributors. The "Contaminating Cell Line", in most cases, will have overgrown the claimed original, or will have
replaced it by a technical error, and the original cells will no longer exist. Table 2 contains cell lines where some stocks have been shown to be misidentified, but where authentic stock is known to exist.
Cell lines are added after examination of cell line provenance and authentication testing data. An effort has been made to exclude synonymous cell lines that were legitimately established from the same donor e.g. where one cell line wasknowingly derived from a parental cell line, or two cell lines were established separately from the same individual.
Observations made in these lists are based on published reports and details obtained from contributors, cell bank websites, and Wikipedia. "Reference PubMed ID" refers to the unique ID number assigned to publications by the PubMed
database (http://www.ncbi.nlm.nih.gov/pubmed/). Attributions in the last two columns indicate where these misidentifications were reported and in no way imply responsibility for the cause by the authors or institutions.
Additional cell line information is available through other databases and resources. "Cellosaurus AC" refers to the unique ID number assigned to cell lines by the Cellosaurus database (http://web.expasy.org/cellosaurus/). Cellosaurus acts
as a cell line knowledge resource with links to many other online resources.
This database of cross-contaminated or misidentified cell lines is curated by the International Cell Line Authentication Committee (ICLAC). Researchers are very welcome to submit additional information regarding misidentified cell lines notlisted here, authentic stocks, or any other relevant information. Confusion may also arise from two different cell lines having the same name; information on these would also be welcome. Please contact info@iclac.org and copy to the
ICLAC Chair, Amanda Capes-Davis (acapdav@gmail.com). The database and more information on ICLAC can be found at:
http://iclac.org/databases/cross-contaminations/
The database was developed by Amanda Capes-Davis and Ian Freshney, and published in 2010. To cite the database or learn more, please refer to:Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, Macleod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (2010) Check your cultures! A list of cross-contaminated or misidentified cell lines. Int J Cancer
127(1): 1-8. PMID: 20143388.
The authors take neither credit nor responsibility for any of the primary observations and have merely attempted to review and collate data previously available on other sites.

Table 1. Misidentified cell lines where no authentic stock is known
Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

1E8 (BLIN-1
derivative)

Leukemia, acutelymphoblastic, B cell
precursor

CVCL_L811

NALM-6

Human

Leukemia, acutelymphoblastic, B cell
precursor

CVCL_0092

Drexler et al, 2003

12592342

2008/C13*5.25 (C13,Human
OV2008/C13)

Ovarian carcinoma

CVCL_0114

ME-180

Human

Cervical carcinoma

CVCL_1401

Korch et al, 2012

22710073

222

Human

Ovarian carcinoma

CVCL_1T15

PA1

Human

Teratocarcinoma

CVCL_0479

Korch et al, 2012

22710073

2474/90

Human

Gastric carcinoma

CVCL_9556

HT-29

Human

Colon carcinoma

CVCL_0320

MacLeod et al, 1999

10508494

2563 (MAC-21)

Human

Lung carcinoma

CVCL_M629

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Nelson-Rees et al,
198164519282957/90

Human

Gastric carcinoma

CVCL_9557

HT-29

Human

Colon carcinoma

CVCL_0320

MacLeod et al, 1999

10508494

3051/80

Human

Gastric carcinoma

CVCL_9558

HT-29

Human

Colon carcinoma

CVCL_0320

MacLeod et al, 1999

10508494

41M

Human

Ovarian carcinoma

CVCL_4993

OAW 28Human

Ovarian carcinoma

CVCL_1614

Wilson et al, 1996

8795574

Glioblastoma

CVCL_1G29

U-251 MG

Human

Glioblastoma

CVCL_0021

CVCL_6872

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

CVCL_6873

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

CVCL_6874

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Human

A172TR3 (U251-TR3) Human
ACC2

Human

ACC3

Human

ACCM

Human

Salivary gland,
adenoid cystic
carcinoma
Salivary gland,
adenoid cystic
carcinoma
Salivary gland,
adenoid cystic
carcinoma

Yip et al, 2009
[correction]
Phuchareon et al,
2009; Zhao et al,
2011
Phuchareon et al,
2009; Zhao et al,
2011
Phuchareon et al,
2009; Zhao et al,
2011

Reference
PubMed ID

19584161
19557180, 21868764

19557180, 21868764

19557180, 21868764

Page 1Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC
Salivary gland,
adenoid cystic
carcinoma
Salivary gland,
adenoid cystic
carcinoma

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

Reference
PubMed ID

CVCL_6875

Unknown

Mouse

Unknown

None

Phuchareon et al,200919557180

CVCL_6876

T-24

Human

Bladder carcinoma

CVCL_0554

Phuchareon et al,200919557180

Lung carcinoma

CVCL_8169

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

MacLeod et al, 1999

10508494

Mosquito

Not specified

CVCL_1R48

UnknownMoth, Antheraea
eucalypti

Not specified

None

Aedes vexans culture Mosquito

Not specified

CVCL_1R46

UnknownMoth, Antheraea
eucalypti

Not specified

None

AG-F

Human

Lymphoma, Hodgkin

CVCL_D101

CCRF-CEM

Human

Leukemia, acutelymphoblastic, T cell

CVCL_0207

Drexler et al, 2003

12592342

AKI

Human

Melanoma

CVCL_8170

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Yoshino et al, 2006

16643607

ALVA-31

Human

Prostate carcinoma

CVCL_4737

PC-3

Human

Prostate carcinoma

CVCL_0035

ALVA-41

Human

Prostate carcinoma

CVCL_4734

PC-3

Human

Prostate carcinoma

CVCL_0035

ALVA-55

Human

Prostate carcinoma

CVCL_4736

PC-3

Human

Prostate carcinoma

CVCL_0035

ALVA-101

Human

Prostate carcinoma

CVCL_4735

PC-3

Human

Prostate carcinoma

CVCL_0035

AO

Human

AmnionCVCL_D631

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Ao38 (BTI-Tnao38)

Black witch moth,
Ascalapha odorata

Eggs, normal

CVCL_Z252

Unknown

Cabbage looper moth,Eggs, normal
Trichoplusia ni

None

Hashimoto et al, 2012 22531032

ARO

Human

CVCL_0144

HT-29

Human

Colon carcinoma

CVCL_0320

Zhao et al, 2011

ARO81-1

Human

CVCL_0144

HT-29

Human

Colon carcinoma

CVCL_0320

Schweppe et al, 2008 18713817

CVCL_0030

Gartler, 1967;
Lavappa et al, 1976;
Nelson-Rees et al,
19814864103, 1250349,
6451928

ACCNS

Human

ACCS

Human

ADLC-5M2

Human

Aedes aegypti ,Suitor's clone

Thyroid, anaplastic
carcinoma
Thyroid, anaplastic
carcinoma

Greene et al, 1972;
Nelson-Rees et al,
1981
Greene et al, 1972;
Nelson-Rees et al,
19814402510, 6451928

4402510, 6451928

van Bokhoven et al,
2001; Varella-Garcia 11304728, 11433521
et al, 2001
van Bokhoven et al,
2001; Pan et al, 2001; 11304728, 11135436,
Varella-Garcia et al, 11433521
2001
van Bokhoven et al,14518029
2003
van Bokhoven et al,14518029
2003
Nelson-Rees et al,
6451928
1981

21868764

AV3

Human

AmnionCVCL_1904

HeLa

Human

Cervicaladenocarcinoma

AZ521

Human

Gastric carcinoma

CVCL_2862

HuTu 80

Human

Duodenal carcinoma

CVCL_1301

JCRB website

No PMID

B2-17

Human

Astrocytoma

CVCL_2864

U-251 MG

Human

Glioblastoma

CVCL_0021

JCRB website

No PMID

BCC1/KMC

Human

Basal cell carcinoma

CVCL_A033

HeLa

Human

CVCL_0030

MacLeod et al, 1999

10508494

BE-13

Human

CVCL_1081

PEER

Human

CVCL_1913

Drexler et al, 2003

12592342

BHP 10-3

Human

CVCL_6278

TPC-1

Human

CVCL_6298

Schweppe et al, 2008 18713817

BHP 14-9

Human

CVCL_6279

M14

Human

Melanoma

CVCL_1395

Schweppe et al, 2008 18713817

BHP 17-10

Human

CVCL_6281

M14

Human

Melanoma

CVCL_1395

Schweppe et al, 2008 18713817

Leukemia, acutelymphoblastic, T cell
Thyroid, papillarycarcinoma
Thyroid, papillarycarcinoma
Thyroid, papillarycarcinoma

Cervicaladenocarcinoma
Leukemia, acutelymphoblastic, T cell
Thyroid, papillarycarcinoma

Page 2

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

BHP 2-7

Human

Thyroid, papillarycarcinoma

CVCL_6283

TPC-1

Human

Thyroid, papillarycarcinoma

CVCL_6298

Schweppe et al, 2008;
18713817, 21868764
Zhao et al, 2011

BHP 5-16

Human

Thyroid, papillarycarcinoma

CVCL_6284

M14

Human

Melanoma

CVCL_1395

Schweppe et al, 2008;
18713817, 21868764
Zhao et al, 2011

BHP 7-13

Human

Thyroid, papillarycarcinoma

CVCL_6285

TPC-1

Human

Thyroid, papillarycarcinoma

CVCL_6298

Schweppe et al, 2008 18713817

BIC-1

Human

Esophageal
adenocarcinoma

CVCL_8092

SW-480, SW-620

Human

Colon carcinoma

CVCL_0546 /CVCL_0547

Boonstra et al, 2010

20075370

BLIN-1

Human

Leukemia, acutelymphoblastic, B cell
precursor

CVCL_8173

NALM-6

Human

Leukemia, acutelymphoblastic, B cell
precursor

CVCL_0092

Drexler et al, 2003

12592342

BM-1604

Human

Prostate carcinoma

CVCL_1968

DU-145

Human

Prostate carcinoma

CVCL_0105

MacLeod et al, 1999

10508494

CVCL_0030

Nelson-Rees and
Flandermeyer, 1977;
Nelson-Rees et al,
1981557237, 6451928

Reference
PubMed ID

BrCA 5

Human

Breast carcinoma

CVCL_D280

HeLa

Human

Cervicaladenocarcinoma

BSCC-93

Human

Skin, squamous cell
carcinoma

CVCL_8157

DJM-1

Human

Skin, squamous cell
carcinoma

CVCL_1172

Yoshino et al, 2006;
ICLAC
correspondence

16643607

BT-B

Human

Bladder carcinoma

CVCL_1858

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

DSMZ website

No PMID

C13

Human

Ovarian carcinoma

CVCL_0114

ME-180

Human

Cervical carcinoma

CVCL_1401

Korch et al, 2012

22710073

C16(MRC-5 derivative)

Human

Lung cells, fetal

CVCL_2322

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

ECACC website

No PMID

C-433

Human

CVCL_1969

RD-ES

Human

Sarcoma (Ewing's)

CVCL_2169

DSMZ website

No PMID

CAC2

Human

CVCL_6883

UnknownRat

Unknown

None

Phuchareon et al,200919557180

CaMa (clone 15)

Human

Breast carcinoma

CVCL_1T14

Unknown

Syrian hamster andmouse

Unknown

None

CaOV

Human

Ovarian carcinoma

CVCL_M091

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Caov-2

Human

Ovarian carcinoma

CVCL_6861

NIH:OVCAR-2

Human

Ovarian carcinoma

CVCL_3941

Korch et al, 2012

22710073

CaVe

Human

Gastric carcinoma

CVCL_8444

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Nelson-Rees et al,
19816451928

CCL3

Human

Chordoma

CVCL_M023

Unknown

Mouse

Unknown

None

Brüderlein et al, 2010 21253487

CH1, CH1cisR

Human

Ovarian carcinoma

CVCL_4992 /CVCL_X012

PA1

Human

Teratocarcinoma

CVCL_0479

Chang liver

Human

Liver, normal hepaticcells

CVCL_0238

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

CHB

Human

Astrocytoma

CVCL_1R45

UnknownRat

Not specified

None

Korch et al, 2012
22710073
Gartler, 1967;
Lavappa et al, 1976; 4864103, 1250349,
Nelson-Rees et al,
6451928
1981
Stoolmiller et al,
1972; Nelson-Rees et 4640072, 6451928
al, 1981

Giant cell tumor,
benignSalivary gland,
adenoid cystic
carcinoma

Nelson-Rees et al,
1981
Nelson-Rees et al,
19816451928
6451928

Page 3

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

CHP-100

Human

Neuroblastoma

CVCL_7166

IMR-32

Human

Neuroblastoma

CVCL_0346

ECACC website

No PMID

CHP-234

Human

Neuroblastoma

CVCL_4359

Unknown

Human

Unknown

None

ATCC website

No PMID

Clom 15

Human

Glioblastoma

CVCL_1R52

UnknownRat

Unknown

None

Higgins et al, 2010

20951163

Clone 1-5c-4

HumanConjunctiva

CVCL_2260

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

ECACC website

No PMID

Clone-16

Human

Pancreas, fetal
endocrine cells

CVCL_1T11

Unknown

Syrian hamster

Unknown

None

Matsuba et al, 1988

2903855

Clone 1A

Rainbow trout,
Blood
Oncorhynchus mykiss

CVCL_1T03

CHSE-214

Chinook salmon,
Oncorhynchus
tschawytscha

Embryo

CVCL_2780

Perry et al, 2001

19002917

CMP

Human

CVCL_D297

HeLa

Human

CMPII C2

Human

CVCL_L115

HeLa

Human

CNDT2

Human

Carcinoid tumour

CVCL_L293

Unknown

Human

Unknown

None

Ellis et al, 2010

20959409

CNE-1

Human

Nasal pharynx,
carcinoma

CVCL_6888

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Chan et al, 2008;
Strong et al, 2014

18196576, 24991015

CNE-2

Human

Nasal pharynx,
carcinoma

CVCL_6889

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Chan et al, 2008;
Strong et al, 2014

18196576, 24991015

CO (= COLE)

Human

Lymphoma, Hodgkin

CVCL_J653

CCRF-CEM

Human

Leukemia, acutelymphoblastic, T cell

CVCL_0207

Drexler et al, 2003

12592342

COLO-587

Human

Pancreatic carcinoma CVCL_F641

COLO-320DM

Human

Colon carcinoma

CVCL_0219

ATCC FAQ #11058

No PMID

COLO-677

Human

Lung carcinoma,
small cell

CVCL_1862

RPMI-8226

Human

Myeloma

CVCL_0014

DSMZ website

No PMID

COLO-818

Human

Melanoma

CVCL_1998

COLO-800

Human

Melanoma

CVCL_1135

MacLeod et al, 1999

10508494

CoLo-TC

Human

Colon carcinoma

CVCL_8290

COLO-205

Human

Colon carcinoma

CVCL_0218

RIKEN website

No PMID

Culisita inornata
cultureMosquito

Not specified

CVCL_1R47

UnknownMoth, Antheraea
eucalypti

Not specified

None

D18T

Human

Synovial cell

CVCL_8669

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

D-54 MG

Human

GliomaCVCL_5735

A-172

Human

Glioblastoma

CVCL_0131

D98/AH

Human

Not specified

CVCL_U961

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Honma et al, 1992

1730567

D98/AH2 Clone B

Human

Not specified

CVCL_2884

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

ECACC website

No PMID

DAMI

Human

Leukemia, acute
myeloid, M7

CVCL_4360

HEL

Human

Leukemia, acute
myeloid, M6

CVCL_0001

MacLeod et al, 1997;
9447816, 12592342
Drexler et al, 2003

DAPT

Human

Astrocytoma, piloid

CVCL_D279

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Nelson-Rees et al,
1981

Rectaladenocarcinoma
Rectaladenocarcinoma

Cervicaladenocarcinoma
Cervicaladenocarcinoma

CVCL_0030
CVCL_0030

Reference
PubMed ID

Gartler, 1967; Nelson4864103, 6451928
Rees et al, 1981
Nelson-Rees et al,
6451928
1981

Greene et al, 1972;
Nelson-Rees et al,
1981
Nelson-Rees et al,
1981
Personal
communication, D.
Bigner4402510, 6451928
6451928
No PMID

6451928

Page 4

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line
DD

Human

Det30A

Human

Detroit-6 (Det6)

Human

Detroit-98

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC
Malignanthistiocytosis
Breast carcinoma,
ascitic fluid

Actual Species

Actual Cell Type
Leukemia, chronic
myeloid, blast crisis
Cervicaladenocarcinoma

Contaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

CVCL_0004

Drexler et al, 2003

CVCL_J651

K-562

Human

CVCL_8674

HeLa

Human

Sternal marrow cells

CVCL_2436

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Human

Sternal marrow cells

CVCL_8188

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Detroit 98/AG

Human

Sternal marrow cells

CVCL_8189

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Detroit 98/AH-2

Human

Sternal marrow cells

CVCL_8190

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Detroit 98/AH-R

Human

Sternal marrow cells

CVCL_8191

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Detroit 98s

Human

Sternal marrow cells

CVCL_8192

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

DM12(Tu-167 derivative)

Human

Oral squamous cell
carcinoma

CVCL_1Q83

UM-SCC-1

Human

DM14
(Tu-167 derivative)

Human

Oral squamous cell
carcinoma

CVCL_1Q84

UM-SCC-1

Human

DRO

Human

CVCL_6286

A-375

Human

DRO90-1

Human

CVCL_6286

A-375

DuPro-1

Human

Prostate carcinoma

CVCL_4738

EB33

Human

Prostate carcinoma

ECC-1

Human

ECTC

Oral squamous cell
carcinoma(recurrence)
Oral squamous cell
carcinoma(recurrence)

CVCL_0030

Nelson-Rees et al,
1981
Gartler, 1967;
Lavappa et al, 1976;
Nelson-Rees et al,
1981
Gartler, 1967;
Lavappa, 1978;
Nelson-Rees et al,1981
Lavappa, 1978;
Nelson-Rees et al,1981
Lavappa, 1978;
Nelson-Rees et al,1981
Lavappa, 1978;
Nelson-Rees et al,1981
Lavappa, 1978;
Nelson-Rees et al,
1981

Reference
PubMed ID
12592342
64519284864103, 1250349,
64519284864103, 566722,
6451928566722, 6451928

566722, 6451928

566722, 6451928

566722, 6451928

CVCL_7707

Zhao et al, 2011

21868764

CVCL_7707

Zhao et al, 2011

21868764

Melanoma

CVCL_0132

Zhao et al, 2011

21868764

Human

Melanoma

CVCL_0132

Schweppe et al, 2008 18713817

PC-3

Human

Prostate carcinoma

CVCL_0035

CVCL_8344

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Endometrial
carcinoma

CVCL_7260

Ishikawa

Human

Endometrial
carcinoma

CVCL_D199

Korch et al, 2012

22710073

Cow

Thyroid, embryonic

CVCL_4518

Vero

Monkey, African
Kidney, normal renal
green (Cercopithecus
cells
aethiops )CVCL_0059

Milanesi et al, 2003

14505435

ECV-304

Human

Endothelium, normal
cells

CVCL_2029

T-24

Human

CVCL_0554

Dirks et al, 1999;
MacLeod et al, 1999

10614862, 10508494

ED27

Human

Chorionic villus

CVCL_8345

HeLa

Human

CVCL_0030

Kniss et al, 2002

11869090

EEK

Horse

Kidney, embryonic
renal cells

CVCL_8346

NSK

Pig, Sus scrofa

CVCL_8378

Milanesi et al, 2003

14505435

EH

Human

Leukemia, hairy cell

CVCL_L804

HK

Human

Leukemia, hairy cell

CVCL_L805

Drexler et al, 2003

12592342

EJ-1

Human

Bladder carcinoma

CVCL_2893

T-24

Human

Bladder carcinoma

CVCL_0554

Masters et al, 2001

11416159

Thyroid, anaplastic
carcinoma
Thyroid, anaplastic
carcinoma

Bladder carcinoma
Cervicaladenocarcinoma
Kidney, normal renalcells

van Bokhoven et al,
2003
Nelson-Rees, 1979;
Nelson-Rees et al,
198114518029
535908, 6451928

Page 5

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

Ej138

Human

Bladder carcinoma

CVCL_2443

T-24

Human

Bladder carcinoma

CVCL_0554

Azari et al, 2007

17254797

ElCo

Human

Breast carcinoma

CVCL_8686

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Nelson-Rees et al,
19816451928

EPC

Carp, Cyprinus carpio Epithelial papilloma

CVCL_4361

Unknown

Fathead minnow,
Unknown
Pimephales promelas

None

ATCC website

No PMID

EPLC3-2M1

HumanLung carcinoma

CVCL_8193

HeLa

Human

CVCL_0030

MacLeod et al, 1999

10508494

EPLC-65

Human

Lung carcinoma

CVCL_8194

HeLa

Human

CVCL_0030

MacLeod et al, 1999

10508494

ESP1

Human

Lymphoma, Burkitt

CVCL_8351

HeLa

Human

CVCL_0030

Nelson-Rees et al,
19816451928

ETK-1

Human

Cholangiocarcinoma

CVCL_1206

SSP-25Human

CVCL_4902

Yoshino et al, 2006

16643607

EU-1

Human

CVCL_8857

REH

Human

CVCL_1650

Drexler et al, 2003

12592342

EU-7

Human

CVCL_8865

CCRF-CEM

Human

CVCL_0207

Drexler et al, 2003

12592342

EUE

Human

Subcutis, fetal

CVCL_7262

HeLa

Human

CVCL_0030

Nelson-Rees et al,
19816451928

EVLC2

Human

Umbilical vein
endothelium,transfected

CVCL_8687

Unknown

Human

Non-endothelial?

None

Unger et al, 2002

12453433

F2-4E5

Human

Thymic epithelium

CVCL_A040

SK-HEP-1

Human

Liver carcinoma

CVCL_0525

MacLeod et al, 1999

10508494

F2-5B6

Human

Thymic epithelium

CVCL_A041

SK-HEP-1

Human

Liver carcinoma

CVCL_0525

MacLeod et al, 1999

10508494

F255A4

Human

Not specified

CVCL_8688

HeLa

Human

CVCL_0030

Nelson-Rees et al,
19816451928

FB2

Human

CVCL_9917

TPC-1

Human

CVCL_6298

Ribeiro et al, 2008

19087340

Fitz-HSA

Dog, Canis familiaris

CVCL_1R34

DEN-HSA

Dog, Canis familiaris

FL

Human

CVCL_1905

HeLa

Human

Flow 13000

Human

MRC-5

Human

Flow 5000

Human

Flow 1000

Human

Flow 6000

Human

Flow 1000

Human

Flow 7000

Human

Flow 3000

Human

FQ

Human

Lymphoma, Hodgkin

CVCL_L984

OMK-210

Monkey, Owl (Aotus
trivirgatus )Kidney, normal renal
cells

G-11
(HBT-3 derivative)

Human

Breast carcinoma

CVCL_U962

HeLa

Human

GHE

Human

Astrocytoma

CVCL_8199

T-24

Human

Leukemia, acutelymphoblastic, B cell
precursor
Leukemia, acutelymphoblastic, T cell

Thyroid, papillarycarcinoma
Sarcoma(hemangiosarcoma)
AmnionLung cells, embryonic
CVCL_F664
fibroblast
Lung cells, embryonic
CVCL_5910
fibroblast
Lung cells, embryonic
CVCL_F662
fibroblast
Lung cells, embryonic
CVCL_2904
fibroblast

Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cholangiocarcinoma
Leukemia, acutelymphoblastic, B cell
precursor
Leukemia, acutelymphoblastic, T cell
Cervicaladenocarcinoma

Cervicaladenocarcinoma
Thyroid, papillarycarcinoma
Sarcoma(hemangiosarcoma)
Cervicaladenocarcinoma

CVCL_1R33
CVCL_0030

Lung cells, embryonic
CVCL_0440
fibroblast
Lung cells, embryonic
CVCL_5615
fibroblast
Lung cells, embryonic
CVCL_5615
fibroblast
Lung cells, embryonic
CVCL_2903
fibroblast

O'Donoghue et al,
2011
Nelson-Rees et al,
1981; Ogura et al,
1993

Reference
PubMed ID

21908323
6451928, 8397027

JCRB website

No PMID

JCRB website

No PMID

JCRB website

No PMID

JCRB website

No PMID

CVCL_L983

Nelson-Rees et al,
1981; Drexler et al,
20036451928, 12592342

Cervicaladenocarcinoma

CVCL_0030

Nelson-Rees et al,
19816451928

Bladder carcinoma

CVCL_0554

MacLeod et al, 1999

10508494

Page 6

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

Reference
PubMed ID

CVCL_0030

Gartler, 1967;
Lavappa, 1978;
Nelson-Rees et al,
19814864103, 566722,
6451928

Girardi heart

Human

Heart, normal cells

CVCL_2254

HeLa

Human

Cervicaladenocarcinoma

GM1312Human

Myeloma

CVCL_J111

Correct name, incorrectcell type

Human

EBV+ Blymphoblastoid cell
line

None

Drexler et al, 2003

12592342

GOS-3

Human

Glioma, mixed astrooligodendroglioma

CVCL_2050

U-343 MG

Human

Glioblastoma

CVCL_4773

DSMZ website

No PMID

GPS-M

Guinea pig

Spleen, adult cells

CVCL_1R31

Strain L-M

Mouse

Connective tissue

CVCL_4535

GPS-PD

Guinea pig

Spleen, adult cells

CVCL_1R32

Strain L-M

Mouse

Connective tissue

CVCL_4535

GREF-X

Human

Liver, hepatic
myofibroblast

CVCL_7667

UnknownRat

Unknown

None

MacLeod et al, 1999

10508494

GT3TKB

Human

Gastric carcinoma

CVCL_1237

RERF-LC-A1

Human

Lung carcinoma

CVCL_4402

Yoshino et al, 2006

16643607

H-494

Human

Prostate carcinoma

CVCL_7669

HeLa

Human

CVCL_0030

Williams, 1980

6244232

CVCL_1T06

HepG2

Human

CVCL_0027

van Pelt et al, 2003

12619888

CVCL_1T07

HepG2

Human

CVCL_0027

van Pelt et al, 2003

12619888

CVCL_1T08

HepG2

Human

CVCL_0027

van Pelt et al, 2003

12619888

CVCL_1T09

HepG2

Human

CVCL_0027

van Pelt et al, 2003

12619888

H7D7A andderivatives
H7D7B andderivatives
H7D7C andderivatives
H7D7D andderivatives

Human
Human
Human
Human

Liver, normal cells(SV40-transformed)
Liver, normal cells(SV40-transformed)
Liver, normal cells(SV40-transformed)
Liver, normal cells(SV40-transformed)

Cervicaladenocarcinoma
Liver, hepatocellular
carcinoma
Liver, hepatocellular
carcinoma
Liver, hepatocellular
carcinoma
Liver, hepatocellular
carcinoma

Nelson-Rees et al,
1981
Nelson-Rees et al,
19816451928
6451928

HAC15

Human

Adrenal gland,
adrenal cortex
carcinoma

CVCL_S898

NCI-H295R

Human

Adrenal gland,
adrenal cortex
carcinoma

CVCL_0458

Wang and Rainey,
201221924324

HAG

Human

Thyroid adenoma(goitre)CVCL_8223

T-24

Human

Bladder carcinoma

CVCL_0554

MacLeod et al, 1999

10508494

HBC

Human

Breast carcinoma,
CVCL_M630
invasive ductal tumor

UnknownRat

Unknown

None

HBL-100

Human

Breast carcinoma

CVCL_4362

Unknown

Human

Unknown

None

HBT-3

Human

Breast carcinoma

CVCL_D281

HeLa

Human

HBT-39b

Human

Breast carcinoma

CVCL_J652

HeLa

Human

HBT-E
(HBT-3 clone)

Human

Breast carcinoma

CVCL_M746

HeLa

Human

HCE

Human

Cervical carcinoma

CVCL_M619

HeLa

Human

CVCL_M850

Hcu-10, Hcu-18, Hcu-22,
Hcu-27, Hcu-33, Hcu-37,
Hcu-39 (synonymous cell Humanlines, donor origin
unknown)

HCu-10Human

Esophageal
squamous cell
carcinoma

Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma

Esophageal
squamous cell
carcinoma

CVCL_0030
CVCL_0030
CVCL_0030
CVCL_0030
CVCL_M850 /CVCL_M852 /CVCL_M853 /CVCL_M854 /CVCL_M855 /CVCL_M856 /CVCL M857

Nelson-Rees and
Flandermeyer, 1977;
Nelson-Rees et al,
1981
Yoshino et al, 2006;
ATCC website
Nelson-Rees et al,
1981
Nelson-Rees et al,
1981
Nelson-Rees et al,
1981
Nelson-Rees et al,
1981

van Helden et al,
1988

557237, 6451928

16643607
6451928
6451928
6451928
6451928

3167823

Page 7

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

CVCL_M852

Hcu-10, Hcu-18, Hcu-22,
Hcu-27, Hcu-33, Hcu-37,
Hcu-39 (synonymous cell Humanlines, donor origin
unknown)

Esophageal
squamous cell
carcinoma

CVCL_M853

Hcu-10, Hcu-18, Hcu-22,
Hcu-27, Hcu-33, Hcu-37,
Hcu-39 (synonymous cell Humanlines, donor origin
unknown)

Esophageal
squamous cell
carcinoma

CVCL_M854

Hcu-10, Hcu-18, Hcu-22,
Hcu-27, Hcu-33, Hcu-37,
Hcu-39 (synonymous cell Humanlines, donor origin
unknown)

Esophageal
squamous cell
carcinoma

CVCL_M855

Hcu-10, Hcu-18, Hcu-22,
Hcu-27, Hcu-33, Hcu-37,
Hcu-39 (synonymous cell Humanlines, donor origin
unknown)

Esophageal
squamous cell
carcinoma

CVCL_M856

Hcu-10, Hcu-18, Hcu-22,
Hcu-27, Hcu-33, Hcu-37,
Hcu-39 (synonymous cell Humanlines, donor origin
unknown)

Esophageal
squamous cell
carcinoma

Esophageal
squamous cell
carcinoma

Human

Bladder carcinoma

Human

Human

Esophageal
squamous cell
carcinoma

Human

Esophageal
squamous cell
carcinoma

Human

Esophageal
squamous cell
carcinoma

Human

Esophageal
squamous cell
carcinoma

Human

Esophageal
squamous cell
carcinomaHCu-39Human

Esophageal
squamous cell
carcinoma

CVCL_M857

Hcu-10, Hcu-18, Hcu-22,
Hcu-27, Hcu-33, Hcu-37,
Hcu-39 (synonymous cell Humanlines, donor origin
unknown)

HCV-29Tmv(HCV-29 derivative)

Human

Bladder, tumorigenic
urothelial cells

CVCL_8229

T-24

HEC-155

Human

Uterineadenocarcinoma

CVCL_2926

HEC-180

Human

Uterineadenocarcinoma

CVCL_8313

HEK

Human

Kidney, embryonic
renal cells

CVCL_M624

HEK/HRV(HEK derivative)

Human

Kidney, transformed
CVCL_M625
embryonic renal cells

HCu-18

HCu-22

HCu-27

HCu-33

HCu-37

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC
CVCL_M850 /CVCL_M852 /CVCL_M853 /CVCL_M854 /CVCL_M855 /CVCL_M856 /CVCL M857
CVCL_M850 /CVCL_M852 /CVCL_M853 /CVCL_M854 /CVCL_M855 /CVCL_M856 /CVCL M857
CVCL_M850 /CVCL_M852 /CVCL_M853 /CVCL_M854 /CVCL_M855 /CVCL_M856 /CVCL M857
CVCL_M850 /CVCL_M852 /CVCL_M853 /CVCL_M854 /CVCL_M855 /CVCL_M856 /CVCL M857
CVCL_M850 /CVCL_M852 /CVCL_M853 /CVCL_M854 /CVCL_M855 /CVCL_M856 /CVCL M857
CVCL_M850 /CVCL_M852 /CVCL_M853 /CVCL_M854 /CVCL_M855 /CVCL_M856 /CVCL M857

Misidentification
Reported By

Reference
PubMed ID

van Helden et al,1988

3167823van Helden et al,1988

3167823van Helden et al,1988

3167823van Helden et al,1988

3167823van Helden et al,1988

3167823van Helden et al,1988

3167823

CVCL_0554

Christensen et al,
1993

8105864

Uterine carcinoma

CVCL_8313 /CVCL_2926

JCRB website

No PMID

Human

Uterineadenocarcinoma

CVCL_8313 /CVCL_2926

JCRB website

No PMID

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Nelson-Rees et al,
19816451928

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Nelson-Rees et al,
19816451928

HEC-155, HEC-180(synonymous cell lines,
donor origin unknown)
HEC-155, HEC-180(synonymous cell lines,
donor origin unknown)

Page 8

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeHEL-R66

Human

Not specified

CVCL_1R39

Unknown

Monkey, African
green (Cercopithecus Unknown
aethiops )HEp-2 (H.Ep.-2)

Human

Laryngeal carcinoma

CVCL_1906

HeLa

Human

Hep-2C

Human

Laryngeal carcinoma

CVCL_2940

HeLa

Human

Hep2 (Clone 2B)

Human

Laryngeal carcinoma

CVCL_2817

HeLa

Human

Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma

Contaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

Reference
PubMed ID

None

Nelson-Rees et al,1981

CVCL_0030

Gartler, 1967; Nelson4864103, 6451928,
Rees et al, 1981;
3180844Chen, 1988

CVCL_0030

ECACC website

No PMID

CVCL_0030

ECACC website

No PMID

CVCL_0030

Korch et al, 2012;
Kniss & Summerfield, 22710073, 24520087
2014

6451928, 7236009

HES

Human

Endometrium

CVCL_W635

HeLa

Human

Cervicaladenocarcinoma

HIMEG-1

Human

Leukemia, chronic
myeloid

CVCL_8439

HL-60

Human

Leukemia, acute
myeloid, M2

CVCL_0002

Drexler et al, 2003

12592342

HKB-1

Human

Lymphoma, Hodgkin

CVCL_5290

Unknown

Human

Unknown

None

Drexler et al, 2003

12592342

HKMUS

Human

Cervical carcinoma

CVCL_8776

SKG-II-SF

Human

Cervical carcinoma

CVCL_8159

RIKEN website

No PMID

HKMUS-SF

Human

Cervical carcinoma

CVCL_8230

SKG-II-SF

Human

Cervical carcinoma

CVCL_8159

Yoshino et al, 2006

16643607

HL111783

Human

Lung carcinoma

CVCL_8231

HeLa

Human

CVCL_0030

Yoshino et al, 2006

16643607

HMV-1

Human

Melanoma

CVCL_8233

HeLa

Human

CVCL_0030

MacLeod et al, 1999

10508494

HPB-MLT

Human

Leukemia, acutelymphoblastic, T cell

CVCL_7959

HPB-ALL

Human

CVCL_1820

Drexler et al, 2003

12592342

HPC-36M(HPC-36 derivative)

Human

Prostate carcinoma

CVCL_8446

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Masters et al, 2001

11416159

hPTC

Human

Thyroid, papillary cell CVCL_8224

Unknown

Pig, Sus scrofa

Unknown

None

MacLeod et al, 1999

10508494

Hs 677.St

Human

Gastric tissue, normal CVCL_F646

Unknown

Mouse

Unknown

None

ATCC FAQ #1072

No PMID

HSC-41

Human

Gastric carcinoma

CVCL_8355

HSC-42

Human

Gastric carcinoma

CVCL_0316

JCRB website

No PMID

HSG

Human

CVCL_2517

HeLa

Human

CVCL_0030

ECACC website

No PMID

HSG-AZA1

Human

CVCL_A047

HeLa

Human

CVCL_0030

JCRB website

No PMID

HSG-AZA3

Human

CVCL_A048

HeLa

Human

CVCL_0030

JCRB website

No PMID

HSGc-C5

Human

Oral carcinoma

CVCL_2951

HeLa

Human

CVCL_0030

JCRB website

No PMID

HS-SULTAN

Human

Myeloma

CVCL_2516

JIJOYE

Human

Lymphoma, Burkitt

CVCL_1317

Drexler et al, 2001;
Drexler et al, 2003

11732505, 12592342

HSY

Human

Salivary gland, parotid CVCL_B032

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

JCRB website

No PMIDhTERT-EEC

Human

MCF-7

Human

Breast carcinoma

CVCL_0031

Korch et al, 2012

22710073

CVCL_8228

Christensen et al,
19937905254

CVCL_0554

Christensen et al,
1993

8105864

Hu1734

Human

Hu456

Human

Salivary gland,
submandibular
Salivary gland,
submandibular
Salivary gland,
submandibularEndometrium,
immortalized
Bladder, nonmalignant urothelial
cells
Bladder, tumorigenic
urothelial cells

CVCL_X007

Cervicaladenocarcinoma
Cervicaladenocarcinoma
Leukemia, acutelymphoblastic, T cell

Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma

CVCL_8451

HCV-29Human

Bladder, nonmalignant urothelial
cells

CVCL_8238

T-24

Human

Bladder carcinoma

Page 9

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line
Hu549

Human

Hu609

Human

Hu609Tmv(Hu609 derivative)

Human

Hu961a, Hu961t(Hu961 derivatives)

Human

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC
Bladder, tumorigenic
urothelial cells
Bladder, nonmalignant urothelial
cells

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

CVCL_8239

T-24

Human

Bladder carcinoma

CVCL_0554

CVCL_8240

J82

Human

Bladder carcinoma

CVCL_0359

Bladder, tumorigenic
urothelial cells

CVCL_8242

T-24

Human

Bladder carcinoma

CVCL_0554

Bladder, tumorigenic
urothelial cells

CVCL_8243

T-24

Human

Bladder carcinoma

CVCL_0554

CVCL_8283

HeLa

Human

CVCL_8357

HeLa

Human

Kidney, normal renal
cells
Liver, hepatocellular
carcinoma

HuK 39

o

Human

HuL-1

Human

Hut

Human

Not specified

CVCL_M861

HeLa

Human

IMC-2

Human

Maxillary carcinoma

CVCL_8245

HeLa

Human

IMC-3

Human

Maxillary carcinoma

CVCL_8246

HeLa

Human

IMC-4

Human

Maxillary carcinoma

CVCL_8247

HeLa

Human

Intestine 407(Int-407, HEI)

Human

Intestinal cells(jejunum/ileum),
embryonic

CVCL_1907

HeLa

IPDDC-A2

Human

Astrocytoma

CVCL_7169

IPRB

Human

Glioblastoma

CVCL_1R53

IPRI-OL-7

White marked tussock
moth, Orgyia
Neonate larvae
leucostigma

CVCL_Z490

IPRI-CF-124

IPRI-OL-11

White marked tussock
moth, Orgyia
Neonate larvae
leucostigma

CVCL_Z489

IPRI-CF-124

IPTP/98

Human

Glioblastoma

CVCL_7168

Unknown

IST-1

Human

Placenta

CVCL_F642

J-111

Human

Leukemia, acute
myeloid, M5

J96

Human

JCA-1
JHC

Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma
Cervicaladenocarcinoma

CVCL_0030

Misidentification
Reported By
Christensen et al,
1993
Christensen et al,
1993; Masters et al,
2001
Christensen et al,
1993
Christensen et al,
1993; Masters et al,
2001
Nelson-Rees et al,
1981

Reference
PubMed ID
8105864
7905254, 11416159

8105864

8105864, 11416159
6451928

CVCL_0030

JCRB website

No PMID

CVCL_0030

Nelson-Rees et al,
19816451928

CVCL_0030

MacLeod et al, 1999

10508494

CVCL_0030

Masters et al, 2001

11416159

CVCL_0030

RIKEN website

No PMID

Human

Cervicaladenocarcinoma

CVCL_0030

Gartler, 1967;
Lavappa, 1978;
Nelson-Rees et al,
19814864103, 566722,
6451928

UnknownRat

Unknown

None

ECACC website

No PMID

UnknownRat

Unknown

None

Higgins et al, 2010

20951163

Neonate larvae

CVCL_Z480

Harvey and Sohi,
1985

No PMID

Neonate larvae

CVCL_Z480

Harvey and Sohi,
1985

No PMID

Rat

Unknown

None

ECACC website

No PMID

SK-OV-3

Human

Ovarian carcinoma

CVCL_0532

ATCC FAQ #1068

No PMID

CVCL_2965

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Gartler, 1967; Nelson4864103, 6451928,
Rees et al, 1981;
12592342
Drexler et al, 2003

Leukemia

CVCL_3990

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Human

Prostate carcinoma

CVCL_4015

T-24

Human

Bladder carcinoma

CVCL_0554

Human

Placenta

CVCL_M093

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Eastern sprucebudworm,
Choristoneura
fumiferana
Eastern sprucebudworm,
Choristoneura
fumiferana

Nelson-Rees et al,
1981
van Bokhoven et al,
2001
Nelson-Rees et al,
19816451928
11522622
6451928

Page 10

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

JHT (JHC derivative) Human

Placenta
Oral squamous cell
carcinoma (lymph
node)
Oral squamous cell
carcinoma (lymph
node)
Oropharyngeal
squamous cell
carcinoma
Oral squamous cell
carcinoma

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

Reference
PubMed ID

Cervical
CVCL_0030
adenocarcinoma
Laryngeal squamouscell carcinoma (lymph CVCL_5991
node)Hypopharyngeal
CVCL_1218
squamous cell
carcinoma

Nelson-Rees et al,
19816451928

Zhao et al, 2011

21868764

Zhao et al, 2011

21868764

Human

Prostate carcinoma

CVCL_0035

Zhao et al, 2011

21868764

A549

Human

Lung carcinoma

CVCL_0023

Zhao et al, 2011

21868764

CVCL_7707

Zhao et al, 2011

21868764

CVCL_M620

HeLa

Human

CVCL_5987

JHU022

Human

CVCL_5988

FaDu

Human

CVCL_5989

PC-3

CVCL_5992

JHU012Human

JHU013 (JHU012
derivative)

Human

JHU019Human

JHU028Human

JMAR(Tu-167 derivative)

Human

Oral squamous cell
carcinoma

CVCL_1Q86

UM-SCC-1

Human

Oral squamous cell
carcinoma(recurrence)

JOSK-I

Human

Leukemia, acute
myeloid, M4

CVCL_2082

U-937

Human

Lymphoma, histiocytic CVCL_0007

MacLeod et al, 1999;
10508494, 12592342
Drexler et al, 2003

JOSK-K

Human

Leukemia, acute
myeloid, M5

CVCL_8141

U-937

Human

Lymphoma, histiocytic CVCL_0007

MacLeod et al, 1999;
10508494, 12592342
Drexler et al, 2003

JOSK-M

Human

Leukemia, chronic
myeloid, blast crisis

CVCL_2083

U-937

Human

Lymphoma, histiocytic CVCL_0007

MacLeod et al, 1999;
10508494, 12592342
Drexler et al, 2003

JOSK-S

Human

Leukemia, acute
myeloid, M5

CVCL_8142

U-937

Human

Lymphoma, histiocytic CVCL_0007

MacLeod et al, 1999;
10508494, 12592342
Drexler et al, 2003

JTC-17

Human

Skin

CVCL_8362

HeLa

Human

JTC-3

Human

Not specified

CVCL_A034

HeLa

Human

K051

Human

Leukemia, acute
myeloid, M2

CVCL_3000

K-562

Human

K1

Human

Thyroid, papillarycarcinoma

CVCL_2537

GLAG-66

K2

Human

Thyroid, papillarycarcinoma

CVCL_6308

KAK1

Human

Thyroid, follicularadenoma

KAT10

Human

KAT4

Human

KAT5

Human

KAT50

Human

CVCL_0030

Honma et al, 1992;
JCRB website

1730567

CVCL_0030

Ogura et al, 1997

9556756

Leukemia, chronic
myeloid, blast crisis

CVCL_0004

Drexler et al, 2003

12592342

Human

Thyroid, papillarycarcinoma

CVCL_9918

Ribeiro et al, 2008;
19087340, 18713817
Schweppe et al, 2008

GLAG-66

Human

Thyroid, papillarycarcinoma

CVCL_9918

Ribeiro et al, 2008;
19087340, 18713817
Schweppe et al, 2008

CVCL_6301

HT-29

Human

Colon carcinoma

CVCL_0320

Thyroid, papillarycarcinoma

CVCL_6302

HT-29

Human

Colon carcinoma

CVCL_0320

Thyroid, anaplastic
carcinoma

CVCL_6304

HT-29

Human

Colon carcinoma

CVCL_0320

HT-29

Human

Colon carcinoma

CVCL_0320

Schweppe et al, 2008 18713817

HT-29

Human

Colon carcinoma

CVCL_0320

Schweppe et al, 2008 18713817

Thyroid, papillary
CVCL_0370
carcinoma
Thyroid, differentiated
CVCL_6305
cells

Cervicaladenocarcinoma
Cervicaladenocarcinoma

van Staveren et al,2007; Schweppe et al, 17804723, 18713817
2008
van Staveren et al,2007; Schweppe et al, 17804723, 18713817
2008
van Staveren et al,2007; Schweppe et al, 17804723, 18713817
2008

Page 11

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

KAT7

Thyroid, benign
follicular hyperplasia

Human

Colon carcinoma

CVCL_0320

Schweppe et al, 2008 18713817

Cervicaladenocarcinoma

CVCL_0030

Gartler, 1967;
Lavappa et al, 1976;
Nelson-Rees et al,
19814864103, 1250349,
6451928

CVCL_0030

DSMZ website

No PMID

CVCL_0030

DSMZ website

No PMID

Human

CVCL_6306

HT-29

Reference
PubMed ID

KB

Human

Oral carcinoma

CVCL_0372

HeLa

Human

KB-3-1

Human

Oral carcinoma

CVCL_2088

HeLa

Human

KB-V1

Human

Oral carcinoma

CVCL_2089

HeLa

Human

KCI-MOH1

Human

Pancreatic carcinoma CVCL_2090

HPAC

Human

Pancreatic carcinoma CVCL_3517

DSMZ website

No PMID

KM20

Human

Colon carcinoma

CVCL_L095

HT-29

Human

Colon carcinoma

CVCL_0320

Roschke et al, 2005

15703300

KM20L2 (KM20
derivative)

Human

Colon carcinoma

CVCL_D889

HT-29

Human

Colon carcinoma

CVCL_0320

Roschke et al, 2005

15703300

KM-3

Human

Leukemia, acutelymphoblastic, B cell
precursor

CVCL_0011

REH

Human

Leukemia, acutelymphoblastic, B cell
precursor

CVCL_1650

Drexler et al, 2003

12592342

KM3

Human

Melanoma

CVCL_1R51

UnknownRat

Not specified

None

Moseley et al, 2003

12740908

KMS-21-BM

Human

Myeloma

CVCL_2991

Unknown

Human

Unknown

None

Drexler et al, 2003

12592342

KMT-2

Human

Umbilical cord blood

CVCL_5301

KG-1

Human

Leukemia

CVCL_0374

Yoshino et al, 2006

16643607

KNS-89Human

Gliosarcoma

CVCL_2800

U-251 MG

Human

Glioblastoma

CVCL_0021

JCRB website

No PMID

KOSC-3

Human

Oral carcinoma

CVCL_2789

Ca9-22

Human

Oral carcinoma

CVCL_1102

JCRB website

No PMID

KP-1N

Human

Pancreatic carcinoma CVCL_3002

PANC-1

Human

Pancreatic carcinoma CVCL_0480

JCRB website

No PMID

KPB-M15

Human

Leukemia, chronic
myeloid, blast crisis

CVCL_5308

KYO-1

Human

Leukemia, chronic
myeloid, blast crisis

CVCL_2095

Drexler et al, 2003

12592342

KPL-1

Human

Breast carcinoma

CVCL_2094

MCF-7

Human

Breast carcinoma

CVCL_0031

DSMZ website

No PMID

KP-P1

Human

Prostate carcinoma

CVCL_D283

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Nelson-Rees et al,
19816451928

KSY-1

Human

Sarcoma (Kaposi)

CVCL_F660

T-24

Human

Bladder carcinoma

CVCL_0554

Stürzl et al, 2012;
ATCC FAQ #1061

22987579

KU7

Human

Bladder carcinoma

CVCL_4714

HeLa

Human

CVCL_0030

Jäger et al, 2013

23500642

KU-YS

Human

Neuroblastoma

CVCL_E131

KU-SN

Human

CVCL_8730

RIKEN website

No PMID

L-132

Human

Lung cells, embryonic CVCL_1908

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Lavappa, 1978;
Nelson-Rees et al,
1981

566722, 6451928

L-41 (J96 derivative)

Human

Leukemia, bonemarrow

CVCL_7170

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

ECACC website

No PMID

LC5 (L-132
derivative)

Human

Lung cells, embryonic CVCL_5G52

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Lavappa, 1978;
Nelson-Rees et al,
1981

566722, 6451928

Cervicaladenocarcinoma
Cervicaladenocarcinoma

Cervicaladenocarcinoma
Neuroectodermal
tumor

Page 12

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

LC5-HIV (L-132
derivative)

Human

Lung cells, embryonic CVCL_5G53

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

LED-Ti

Human

Cervical carcinoma

CVCL_8438

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

LLC-15MB

Human

Breast carcinoma

CVCL_B055

M14

Human

Melanoma

CVCL_1395

Thompson et al, 2004 15679051

LN-319Human

Glioma, anaplastic
astrocytoma

CVCL_3958

LN-992

Human

Glioblastoma

CVCL_6845

Bady et al, 2012

22570425

LN-443

Human

Glioblastoma

CVCL_3960

LN-444

Human

CVCL_3961

Bady et al, 2012

22570425

LR10.6

Human

Leukemia, acutelymphoblastic, B cell
precursor

CVCL_8260

NALM-6Human

CVCL_0092

MacLeod et al, 1999;
10508494, 12592342
Drexler et al, 2003

LT-1

Frog, grass

Kidney, renal
adenocarcinoma

CVCL_1R49

TH and FHM

TH = box turtle; FHM= fathead minnow

CVCL_3820 /CVCL_4324

Nelson-Rees et al,
1981

LU

Human

Lung cells, fetal

CVCL_M631

HeLa

Human

LU 106

Human

Lung cells, embryonic CVCL_8892

HeLa

Human

Lu-130

Human

Lung carcinoma

CVCL_6862

Lu-134A, B

HumanLung carcinoma

CVCL_1387 /CVCL_1388

Yoshino et al, 2006

16643607

M10T

Human

Synovial cell

CVCL_M094

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Nelson-Rees et al,
19816451928

M4A4 and derivatives(MDA-MB-435
Human
derivatives)

Breast carcinoma

CVCL_B057

M14

Human

Melanoma

CVCL_1395

ATCC website

No PMID

MA-1

Human

Lymphoma, MALT

CVCL_M665

Pfeiffer

Human

Lymphoma, nonHodgkin

CVCL_3326

MA-104

Monkey, Rhesus(Macaca mulatta )Kidney, embryonic
renal cells

CVCL_3845

Unknown, possibly VeroMonkey, African
Kidney, normal renal
green (Cercopithecus
cells
aethiops )

None

None

Glioblastoma,
recurrence (different
donor)
Leukemia, acutelymphoblastic, B cell
precursor
TH = heart; FHM =unspecified
Cervicaladenocarcinoma
Cervicaladenocarcinoma

CVCL_0030
CVCL_0030

MA-111

RabbitKidney, newborn

CVCL_U963

Unknown, possibly VeroMonkey, African
Kidney, normal renal
green (Cercopithecus
cells
aethiops )MA-160Human

Prostate adenoma

CVCL_8261

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Mash-1

Human

Schwannoma

CVCL_8158

MMac

Human

Melanoma

CVCL_5951

MaTu

Human

Breast carcinoma

CVCL_5328

HeLa

Human

MC-4000

Human

Breast carcinoma

CVCL_5331

HeLa

Human

McCoy

Human

Not specified

CVCL_3742

Strain L

Mouse

Connective tissue

CVCL_0462

MCF-7/AdrR(NCI/ADR-RES)

HumanBreast carcinoma

CVCL_1452

OVCAR-8

Human

Ovarian carcinoma

CVCL_1629

Cervicaladenocarcinoma
Cervicaladenocarcinoma

Misidentification
Reported By
Lavappa, 1978;
Nelson-Rees et al,
1981
Nelson-Rees et al,
1981

Nelson-Rees et al,
1981
Nelson-Rees et al,
1981

Capes-Davis et al,
2013
Whitaker andHayward, 1985;
Milanesi et al, 2003;
ATCC FAQ #1067
Nelson-Rees et al,
1981
Nelson-Rees, 1977;
Nelson-Rees et al,
1981
Yoshino et al, 2006;
ICLAC
correspondence

Reference
PubMed ID
566722, 6451928
6451928

6451928
6451928
645192823907998

4043530, 14505435

6451928562836, 6451928

16643607

CVCL_0030

MacLeod et al, 1999

10508494

CVCL_0030

MacLeod et al, 1999

10508494

Nelson-Rees et al,
6451928
1981
Liscovitch and Ravid,
16504380
2007

Page 13

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

Reference
PubMed ID

MDA-MB-435

Human

Breast carcinoma

CVCL_0417

M14

Human

Melanoma

CVCL_1395

Ellison et al, 2002;
12354931, 17786032,
Christgen et al, 2007;
17004106
Rae et al, 2007

MDA-N
(MDA-MB-435
derivative)

Human

Breast carcinoma,
HER2/ERBB2transfected

CVCL_1910

M14

Human

Melanoma

CVCL_1395

Lorenzi et al, 2009

19372543

MDS

Human

Leukemia, chronic
myelomonocytic

CVCL_L807

JURKAT

Human

Leukemia, acutelymphoblastic, T cell

CVCL_0065

Drexler et al, 2003

12592342

MEL-HO

Human

Melanoma

CVCL_1402

Human

Unknown

CVCL_1402 /CVCL_3981

MacLeod et al, 1999

10508494

MEL-WEI

Human

Melanoma

CVCL_3981

Human

Unknown

CVCL_1402 /CVCL_3981

MacLeod et al, 1999

10508494

MGH-U1 (EJ)

Human

Bladder carcinoma

CVCL_2443

T-24

Human

Bladder carcinoma

CVCL_0554

MGH-U2 (HM)

Human

Bladder carcinoma

CVCL_9826

T-24

Human

Bladder carcinoma

CVCL_0554

MHH-225

Human

Leukemia, acute
myeloid, M7

CVCL_8894

JURKAT

Human

Leukemia, acutelymphoblastic, T cell

CVCL_0065

Drexler et al, 2003

12592342

Minnesota EE

Human

Esophageal
epithelium

CVCL_8264

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Gartler, 1967;
Lavappa, 1978;
Nelson-Rees et al,
19814864103, 566722,
6451928

MKB-1

Human

Leukemia, acute
myeloid

CVCL_8265

CCRF-CEM

Human

Leukemia, acutelymphoblastic, T cell

CVCL_0207

MacLeod et al, 1999;
10508494, 12592342
Drexler et al, 2003

MKN28

Human

Gastric carcinoma

CVCL_1416

MKN74

Human

Gastric carcinoma

CVCL_2791

JCRB website

No PMID

MOBS-1

Human

Leukemia, acute
myeloid, M5

CVCL_8442

U-937

Human

Lymphoma, histiocytic CVCL_0007

Drexler et al, 2003

12592342

MOLT-15

Human

Leukemia, acutelymphoblastic, T cell

CVCL_8150

CTV-1

Human

Leukemia, acute
myeloid, M5

MacLeod et al, 1999;
10508494, 12592342
Drexler et al, 2003

MPanc-96

Human

Pancreatic carcinoma CVCL_7165

AsPC-1

Human

Pancreatic carcinoma CVCL_0152

ATCC website

MRO87-1

Human

CVCL_6310

HT-29

Human

Colon carcinoma

CVCL_0320

Schweppe et al, 2008 18713817

CVCL_4243

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Milanesi et al, 2003

14505435MS(Monkey Stable)
MT-1 [Multiple celllines with same
name, see
Cellosaurus]
MT-3 [Multiple celllines with same
name, see
Cellosaurus]
MUTZ-1

MonkeyThyroid, follicular
carcinoma
Kidney, normal renalcells

MEL-HO, MEL-WEI(synonymous cell lines,
donor origin unknown)
MEL-HO, MEL-WEI(synonymous cell lines,
donor origin unknown)

CVCL_1150

O'Toole et al, 1983;
Lin et al, 1985
O'Toole et al, 1983;
Lin et al, 1985

6823318, 4027986
6823318, 4027986

No PMID

Human

Breast carcinoma

CVCL_0441

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

MacLeod et al, 1999

10508494

Human

Breast carcinoma

CVCL_2129

LS-174T (LS-180
derivative)

Human

Colon carcinoma

CVCL_1384

Yu et al, 2015

25877200

Human

Leukemia, acute
myeloid, M2

CVCL_1431

Namalwa

Human

Lymphoma, Burkitt

CVCL_0067

Drexler et al, 2009

19344951

Page 14

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

MV522

Human

Lung carcinoma

CVCL_7204

HT-29

Human

Colon carcinoma

CVCL_0320

ATCC FAQ #1075

No PMID

NC-37

Human

Lymphoblastoid cellline, normal donor

CVCL_3042

Raji

Human

Lymphoma, Burkitt

CVCL_0511

JCRB website

No PMID

NCC16

Human

Cervical carcinoma

CVCL_U219

PHK16-0b

HumanSkin, immortalised
keratinocytes

CVCL_3123

JCRB website

No PMID

NCI-H1264

Human

Lung carcinoma

CVCL_3970

NCI-H157, NCI-H1264(synonymous cell lines,
donor origin unknown)

Human

Lung carcinoma

CVCL_0463 /CVCL_3970

ATCC FAQ #930,#931

No PMID

NCI-H1304

Human

Lung carcinoma,
small cell

CVCL_1462

NCI-H1304, NCI-H1870(synonymous cell lines,
donor origin unknown)

Human

Lung carcinoma,
small cell

CVCL_1502 /CVCL_1462

ATCC FAQ #1069

No PMID

NCI-H1514

Human

Lung carcinoma

CVCL_4363

Unknown

Human

Unknown

None

Durkin et al, 2000;
ATCC website

10949990

NCI-H157

Human

Lung carcinoma

CVCL_0463

Human

Lung carcinoma

CVCL_0463 /CVCL_3970

ATCC FAQ #930,#931

No PMID

NCI-H1622

Human

Lung carcinoma

CVCL_4364

Human

Unknown

None

ATCC website

No PMID

NCI-H1870

Human

Lung carcinoma,
small cell

CVCL_1502

NCI-H1304, NCI-H1870(synonymous cell lines,
donor origin unknown)

Human

Lung carcinoma,
small cell

CVCL_1502 /CVCL_1462

ATCC FAQ #1069

No PMID

NCI-H249

Human

Lung carcinoma,
small cell

CVCL_A537

NCI-H69

Human

Lung carcinoma,
small cell

CVCL_1579

ATCC FAQ #1073

No PMID

NCI-H513

Human

Mesothelioma

CVCL_A570

NCI-H125

Human

Lung carcinoma

CVCL_3968

ATCC FAQ #1060

No PMID

NCI-H592

Human

CVCL_A590

NCI-H69

Human

CVCL_1579

ATCC FAQ #1070,#1073

No PMID

NCI-H60

Human

CVCL_A592

NCI-N417

Human

CVCL_1602

ATCC FAQ #1064

No PMID

NCI-H630

Human

Metastatic sample

CVCL_1572

MixtureHuman

Unknown

None

ATCC FAQ #1065

No PMID

NCI-H738

Human

Unknown

Human

Unknown

None

ATCC website

No PMID

NCOL-1

Human

Lung carcinoma
CVCL_4365
Intestinal cells, normal
CVCL_8375
colon

LoVo

Human

Colon carcinoma

CVCL_0399

Melcher et al, 2005

15771911

CVCL_0030

Lung carcinoma,
small cell
Lung carcinoma,
small cell

NCI-H157, NCI-H1264(synonymous cell lines,
donor origin unknown)
Unknown

Lung carcinoma,
small cell
Lung carcinoma,
small cell

Gartler, 1967;
Lavappa, 1978;
Nelson-Rees et al,1981
Lavappa, 1978;
Nelson-Rees et al,
1981
van Bokhoven et al,
2001

Reference
PubMed ID

NCTC 2544

Human

Skin epithelium

CVCL_0461

HeLa

Human

Cervicaladenocarcinoma

NCTC 3075

Human

Not specified

CVCL_8156

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

ND-1

Human

Prostate carcinoma

CVCL_4781

DU-145

Human

Prostate carcinoma

CVCL_0105

NM2C5 andderivatives(MDA-MB-435
derivatives)

Human

Breast carcinoma

CVCL_B064

M14

Human

Melanoma

CVCL_1395

ATCC website

No PMID

NOI-90Human

Lymphoma, nonHodgkin, natural killer CVCL_8462
cell

REH

Human

Leukemia, acutelymphoblastic, B cell
precursor

CVCL_1650

Drexler et al, 2003

12592342

NOSE06

Human

Ovary, normal

CVCL_X010

DOV-13

Human

Ovarian carcinoma

CVCL_6774

Korch et al, 2012

22710073

NOSE07

Human

Ovary, normal

CVCL_X011

DOV-13

Human

Ovarian carcinoma

CVCL_6774

Korch et al, 2012

22710073

4864103, 566722,
6451928566722, 6451928
11304728

Page 15

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

NPA87

Human

Thyroid, papillarycarcinoma

CVCL_0467

M14

Human

Melanoma

CVCL_1395

Schweppe et al, 2008;
18713817, 21868764
Zhao et al, 2011

NS-3

Human

Gastric carcinoma

CVCL_8377

COLO 201

Human

Colon carcinoma

CVCL_1987

JCRB website

OCM-3

Human

CVCL_6937

SK-MEL-28

Human

OCM-8

Human

CVCL_6938

SK-MEL-28

Human

OCUM-6

Human

Gastric carcinoma

CVCL_8387

OCUM-11

Human

Gastric carcinoma

CVCL_8379

JCRB website

No PMID

OE

Human

Endometrium

CVCL_J350

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Nelson-Rees et al,
1981; Ogura et al,
19976451928, 9556756

OF

Human

Not specified

CVCL_M621

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Ogura et al, 1997

9556756

OLGA-PH-J/92(OL-J/92)

Crayfish, Orconectes Brain, cerebral
limosusganglion

CVCL_U272

Unknown

Unknown, similar toamoeba species

Unknown

None

Lee et al, 2011; ATCC
21938590
website

ONCO-DG-1

Human

CVCL_1882

OVCAR-3

Human

Ovarian carcinoma

CVCL_0465

DSMZ website

OS 187

Human

CVCL_1R50

HCT-15

Human

Colon carcinoma

CVCL_0292

OST

Human

CVCL_8399

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

OU-AML-1

Human

CVCL_8391

OCI/AML2

Human

OU-AML-2

Human

CVCL_8392

OCI/AML2

Human

OU-AML-3

Human

CVCL_8393

OCI/AML2

Human

OU-AML-4

Human

CVCL_8394

OCI/AML2

Human

OU-AML-5

Human

CVCL_8395

OCI/AML2

Human

OU-AML-6

Human

CVCL_8396

OCI/AML2

Human

OU-AML-7

Human

CVCL_8397

OCI/AML2

Human

OU-AML-8

Human

CVCL_8398

OCI/AML2

Human

Melanoma, primaryuveal
Melanoma, primaryuveal

Thyroid, papillarycarcinoma
Sarcoma(osteosarcoma)
Sarcoma(osteosarcoma)
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M2
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M2
Leukemia, acute
myeloid, M5
Leukemia, acute
myeloid, M1
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4

Melanoma, primaryskin
Melanoma, primaryskin

Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid, M4

CVCL_0526
CVCL_0526

Folberg et al, 2008;
Griewank et al, 2012
Folberg et al, 2008;
Griewank et al, 2012

Zhang et al, 2013
[retraction]
Personal
communication, R.
NardoneReference
PubMed ID

No PMID
18689700, 22236444
18689700, 22236444

No PMID
24046071
No PMID

CVCL_1619

Drexler et al, 2003

12592342

CVCL_1619

Drexler et al, 2003

12592342

CVCL_1619

Drexler et al, 2003

12592342

CVCL_1619

Drexler et al, 2003

12592342

CVCL_1619

Drexler et al, 2003

12592342

CVCL_1619

Drexler et al, 2003

12592342

CVCL_1619

Drexler et al, 2003

12592342

CVCL_1619

Drexler et al, 2003

12592342

No PMID

OV-1063

Human

Ovarian carcinoma

CVCL_4366

DU-145

Human

Prostate carcinoma

CVCL_0105

ATCC website;
personal
communication, E.
Hall

OV2008 (A2008)

Human

Ovarian carcinoma

CVCL_0473

ME-180

Human

Cervical carcinoma

CVCL_1401

Korch et al, 2012

22710073

Ovary1847

Human

Ovarian carcinoma

CVCL_D703

NIH:OVCAR-8

Human

Ovarian carcinoma

CVCL_1629

Korch et al, 2012

22710073

OVMIU

Human

Ovarian carcinoma

CVCL_3112

OVSAYO

Human

Ovarian carcinoma

CVCL_3115

JCRB website

No PMID

Page 16

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

P1-1A3

Human

Thymic epithelium

CVCL_A042

SK-HEP-1

Human

Liver carcinoma

CVCL_0525

MacLeod et al, 1999

10508494

P1-4D6

Human

Thymic epithelium

CVCL_A046

SK-HEP-1

Human

Liver carcinoma

CVCL_0525

MacLeod et al, 1999

10508494

P39/TSUGANE(P39/TSU)

Human

Leukemia, acute
myeloid, M2

CVCL_0478

HL-60

Human

Leukemia, acute
myeloid, M2

CVCL_0002

Drexler et al, 2003;
JCRB website

12592342

Panc 01.28

Human

Pancreatic
adenocarcinoma

CVCL_7666

Panc 01.28, Panc 06.03(synonymous cell lines,
donor origin unknown)

Human

Pancreatic
adenocarcinoma

CVCL_7666 /CVCL_7665

Yu et al, 2015

25877200

Panc 06.03

Human

Pancreatic
adenocarcinoma

CVCL_7665

Panc 01.28, Panc 06.03(synonymous cell lines,
donor origin unknown)

Human

Pancreatic
adenocarcinoma

CVCL_7666 /CVCL_7665

Yu et al, 2015

25877200

PBEI

Human

Leukemia, acutelymphoblastic, B cell
precursor

CVCL_8270

NALM-6Human

CVCL_0092

MacLeod et al, 1999;
10508494, 12592342
Drexler et al, 2003

PC-93

Human

Prostate carcinoma

CVCL_4888

HeLa

Human

CVCL_0030

van Bokhoven et al,200314518029

PC-MDS

Human

Myelodysplastic
syndrome, therapyrelated

CVCL_1Q81

K-562

Human

Leukemia, chronic
myeloid, blast crisis

CVCL_0004

Drexler et al, 2009

19344951

PCI-22A

Human

Oral squamous cell
carcinoma

CVCL_1T56

Unknown

None

Zhao et al, 2011

21868764

PCI-22B

Human

Unknown

None

Zhao et al, 2011

21868764

PCI-3

Human

Human

Prostate carcinoma

CVCL_0035

Zhao et al, 2011

21868764

CVCL_0317

van Bokhoven, 2004;
11433418
Schmelz et al, 2001
[erratum][erratum]

Oral squamous cell
carcinoma (lymph
node)
Oropharyngeal
squamous cell
carcinoma

CVCL_1T57

CVCL_C169

Unknown, does not matchother cell line from same Human
donor
Unknown, does not matchother cell line from same Human
donor
PC-3

Leukemia, acutelymphoblastic, B cell
precursor
Cervicaladenocarcinoma

PEAZ-1

Human

Prostate carcinoma

CVCL_8437

HT-1080

Human

Sarcoma(fibrosarcoma)

PH61-N

Human

Not specified

CVCL_D282

MIA PaCa-2

Human

Pancreatic carcinoma CVCL_0428

PLB-985

Human

CVCL_2162

HL-60

Human

PPC-1

Human

CVCL_4778

PC-3

Human

PSV811

Human

CVCL_3142

WI-38

Human

RAMAK-1

Human

Muscle synovium

CVCL_8271

T-24

Human

RB

Human

Lymphoma, Hodgkin

CVCL_L985

OMK-210

RBHF-1

Human

Liver, hepatoma

CVCL_Y465

RC-2A

Human

RED-3

Human

Leukemia, acute
myeloid, M4
Prostate carcinoma
Skin fibroblast,Werner's syndrome

Leukemia, acute
myeloid, M4
Leukemia, acute
myeloid

Reference
PubMed ID

JCRB website

No PMID

CVCL_0002

Drexler et al, 2003

12592342

CVCL_0035

Chen, 1993

8428522

CVCL_0579

JCRB website

No PMID

Bladder carcinoma

CVCL_0554

MacLeod et al, 1999

10508494

Monkey, Owl (Aotus
trivirgatus )Kidney, normal renal
cells

CVCL_L983

Nelson-Rees et al,
1981; Drexler et al,
20036451928, 12592342

Unknown

Non-human

Unknown

None

MacLeod et al, 1999

10508494

CVCL_L808

CCRF-CEM

Human

CVCL_0207

Drexler et al, 2003

12592342

CVCL_8907

HL-60

Human

CVCL_0002

Drexler et al, 2003

12592342

Leukemia, acute
myeloid, M2
Prostate carcinoma
Lung, normal diploidfibroblasts

Leukemia, acutelymphoblastic, T cell
Leukemia, acute
myeloid, M2

Page 17

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

REH-6

Human

REPC

Human

RERF-LC-MA

Human

RERF-LC-OK

Human

Leukemia, acutelymphoblastic, B cell
precursor
Kidney, normal renalcells
Lung carcinoma,
small cell
Lung carcinoma

RGC-5

Rat

RM-10

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By
Drexler et al, 2003

CVCL_L803

Unknown

Mouse

Unknown

None

CVCL_W815

Hep 3B

Human

Liver, hepatocellular
carcinoma

CVCL_0326

CVCL_3153

SK-MES-1

HumanLung, squamous cell

CVCL_0630

CVCL_3154

MarcusHuman

Astrocytoma

CVCL_3019

Retinal ganglion

CVCL_4059

661W

Mouse

Retina, photoreceptor
CVCL_6240
cells

Human

Leukemia, chronic
myeloid, blast crisis

CVCL_8463

K-562

Human

RMUG-L

Human

Ovarian carcinoma

CVCL_3157

SNG-II

Human

RO-D81-1

Human

CVCL_M779

HT-29

Human

RO-H85-1

Human

CVCL_A666

647-V

Human

RPMI-4788

Human

Colon carcinoma

CVCL_0U46

HeLa

Human

RPMI-6666

Human

Lymphoma, Hodgkin

CVCL_1665

Correct name, incorrectcell type

Human

RPTC-1

Human

CVCL_V277

TPC-1

Human

RS-1

Human

CVCL_8423

K-562

Human

RTSG

Human

Ovarian carcinoma

CVCL_1671

SNG-II

Human

RY

Human

Lymphoma, Hodgkin

CVCL_U964

Unknown

MonkeySA4

Human

CVCL_8910

HeLa

Human

SAM-1

Human

CVCL_8440

K-562

Human

SAML-1

Human

Lymphoma, Hodgkin

CVCL_9997

U-937

Human

SBC-2

Human

Bladder carcinoma

CVCL_1677

HeLa

Human

SBC-7

Human

Bladder carcinoma

CVCL_1680

HeLa

Human

SCCTF

Human

CVCL_0517

SCCKN

Human

SCLC-16H

Human

CVCL_X025

SCLC-21/22H

Human

SCLC-24H

Human

CVCL_8262

SCLC-21/22H

Human

SEG-1

Human

Esophageal
adenocarcinoma

CVCL_8113

NCI-H460

Human

SF767

Human

Glioma, anaplastic
astrocytoma

CVCL_6950

ME-180

SH-2

Human

Breast carcinoma

CVCL_M622

HeLa

Thyroid, medullarycarcinoma
Thyroid, medullarycarcinoma

Thyroid, papillarycarcinoma
Leukemia, acute
myeloid, M7

Sarcoma(liposarcoma)
Leukemia, chronic
myeloid, blast crisis

Oral squamous cell
carcinoma
Lung carcinoma,
small cell
Lung carcinoma,
small cell

Leukemia, chronic
myeloid, blast crisis
Endometrial
carcinoma

Frede et al, 2014
[retraction]
Capes-Davis et al,
2013
JCRB website

Reference
PubMed ID
12592342
21406725
23136038
No PMID

Van Bergen et al,
2009; Krishnamoorthy 19443730, 23975727
et al, 2013

CVCL_0004

Drexler et al, 2003

12592342

CVCL_3170

JCRB website

No PMID

Colon carcinoma

CVCL_0320

Dadon et al, 2013

23472229

Bladder carcinoma

CVCL_1049

Dadon et al, 2013

23472229

CVCL_0030

Capes-Davis et al,
201323136038

None

Drexler et al, 2003

12592342

CVCL_6298

Zhao et al, 2011

21868764

CVCL_0004

Drexler et al, 2003

12592342

CVCL_3170

JCRB website

No PMID

Cervicaladenocarcinoma
EBV+ Blymphoblastoid cellline
Thyroid, papillarycarcinoma
Leukemia, chronic
myeloid, blast crisis
Endometrial
carcinoma
Unknown
Cervicaladenocarcinoma
Leukemia, chronic
myeloid, blast crisis

None
CVCL_0030

Harris et al, 1981;
Drexler et al, 2003
Nelson-Rees et al,
19817192801, 12592342
6451928

CVCL_0004

Drexler et al, 2003

12592342

Lymphoma, histiocytic CVCL_0007

ATCC FAQ #1074

No PMID

CVCL_0030

MacLeod et al, 1999

10508494

CVCL_0030

MacLeod et al, 1999

10508494

CVCL_5362

Yoshino et al, 2006

16643607

MacLeod et al, 1999

10508494

MacLeod et al, 1999

10508494

Lung carcinoma, large
CVCL_0459
cell

Boonstra et al, 2010

20075370

Human

Cervical carcinoma

CVCL_1401

Bady et al, 2012

22570425

Human

Cervicaladenocarcinoma

CVCL_0030

Nelson-Rees et al,
19816451928

Cervicaladenocarcinoma
Cervicaladenocarcinoma
Oral squamous cell
carcinoma
Lung carcinoma,
small cell
Lung carcinoma,
small cell

CVCL_0024 /CVCL_2186
CVCL_0024 /CVCL_2186

Page 18

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

SH-3

Human

Breast carcinoma

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Nelson-Rees et al,
1981

SJPL

Pig, Sus scrofa

Lung cells,
immortalized
epithelial

CVCL_1T04

Unknown

Monkey, African
green (Cercopithecus Unknown
aethiops )None

Silversides et al, 2010 20200241

SK-GT-5

Human

Esophageal
adenocarcinoma

CVCL_8114

SK-GT-2

Human

Gastric carcinoma(gastric fundus)

CVCL_2194

Boonstra et al, 2010

20075370

SK-MG-1

Human

Astrocytoma

CVCL_1698

MarcusHuman

Astrocytoma

CVCL_3019

JCRB website

No PMID

SK-N-MC

Human

Neuroblastoma

CVCL_0530

Unknown

Human

Sarcoma (Ewing's)

None

Staege et al, 2004

15548687

SK-OV-4

Human

Ovarian carcinoma

CVCL_X008

C-33A

Human

Cervical carcinoma

CVCL_1094

Korch et al, 2012

22710073

SK-OV-6

Human

Ovarian carcinoma

CVCL_A457

C-33A

Human

Cervical carcinoma

CVCL_1094

Korch et al, 2012

22710073

SKW-3

Human

Leukemia, chronic
lymphocytic, T cell

CVCL_2197

KE-37

Human

Leukemia, acutelymphoblastic

CVCL_1327

DSMZ website

No PMID

SLK

Human

Sarcoma (Kaposi)

CVCL_9569

Caki-1

Human

Renal cell carcinoma CVCL_0234

Stürzl et al, 2012

22987579

SNB-19Human

Glioblastoma

CVCL_0535

U-251 MG

Human

Glioblastoma

Azari et al, 2007;
ATCC website

17254797

SPI-801

Human

CVCL_2200

K-562

Human

SPI-802

Human

CVCL_2201

K-562

Human

SpR

Human

Lymphoma, Hodgkin

CVCL_L986

OMK-210

Monkey, Owl (Aotus
trivirgatus )

SQ-5

Human

Lung carcinoma

CVCL_8273

HeLa

Human

SR-91

Human

Leukemia, acutelymphoblastic, T cell

CVCL_8441

AML-193

Human

SW-527

Human

Colon carcinoma

CVCL_3799

SW-480, SW-620

Human

Colon carcinoma

SW-598

Human

Colon carcinoma

CVCL_F649

SW-480, SW-620

Human

Colon carcinoma

SW-608

Human

Colon carcinoma

CVCL_F653

SW-480, SW-620

Human

Colon carcinoma

SW-613

Human

Colon carcinoma

CVCL_F650

SW-480, SW-620

Human

Colon carcinoma

SW-732

Human

Colon carcinoma

CVCL_F651

SW-480, SW-620

Human

Colon carcinoma

SW-733

Human

Colon carcinoma

CVCL_F652

SW-480, SW-620

Human

Colon carcinoma

T-1

Human

Kidney, normal renal
cells

CVCL_M858

HeLa

Human

Cervicaladenocarcinoma

T1

Human

Neural stem cell

CVCL_1U15

HeLa

Human

T-33

Human

Leukemia, chronic
myeloid, blast crisis

CVCL_8427

K-562

Human

Leukemia, acutelymphoblastic, T cell
Leukemia, acutelymphoblastic, T cell

CVCL_M383

Leukemia, chronic
myeloid, blast crisis
Leukemia, chronic
myeloid, blast crisis
Kidney, normal renal
cells
Cervicaladenocarcinoma
Leukemia, acute
myeloid, M5

Cervicaladenocarcinoma
Leukemia, chronic
myeloid, blast crisis

CVCL_0021
CVCL_0004
CVCL_0004
CVCL_L983

Gignac et al, 1993;
Drexler et al, 2003
Gignac et al, 1993;
Drexler et al, 2003
Nelson-Rees et al,
1981; Drexler et al,
2003

Reference
PubMed ID
64519288220135, 12592342
8220135, 12592342
6451928, 12592342

CVCL_0030

Yoshino et al, 2006

16643607

CVCL_1071

Drexler et al, 2003

12592342

CVCL_0546 /CVCL_0547
CVCL_0546 /CVCL_0547
CVCL_0546 /CVCL_0547
CVCL_0546 /CVCL_0547
CVCL_0546 /CVCL_0547
CVCL_0546 /CVCL_0547

Nelson-Rees et al,
6451928
1981
Nelson-Rees et al,
6451928
1981
Nelson-Rees et al,
6451928
1981
Nelson-Rees et al,
6451928
1981
Nelson-Rees et al,
6451928
1981
Nelson-Rees et al,
6451928
1981
Nelson-Rees et al,
1980; Nelson-Rees et 7394535, 6451928
al, 1981

CVCL_0030
CVCL_0030

Torsvik et al, 2012

22949886

CVCL_0004

Drexler et al, 2003

12592342

Page 19

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

T404

Human

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Oral squamous cell
carcinoma

Actual Species

Actual Cell Type

CVCL_1T20

T404, T406, Tu-138, Tu158LN, Tu-159, Tu-182,
Tu-212, Tu-212LN(synonymous cell lines,
donor origin unknown)

Human

Head and necksquamous cell
carcinoma

Human

Head and necksquamous cell
carcinoma

Contaminating Cell
Line, Cellosaurus AC
CVCL_1T20 /CVCL_1T19 /CVCL_4910 /CVCL_1T17 /CVCL_4911 /CVCL_4914 /CVCL_4915 /CVCL 1T18
CVCL_1T20 /CVCL_1T19 /CVCL_4910 /CVCL_1T17 /CVCL_4911 /CVCL_4914 /CVCL_4915 /CVCL 1T18

Misidentification
Reported By

Reference
PubMed ID

Zhao et al, 2011

21868764

Zhao et al, 2011

21868764

T406[Multiple cell lines
Humanwith same name, see
Cellosaurus]

Oral squamous cell
carcinoma

CVCL_1T19

T404, T406, Tu-138, Tu158LN, Tu-159, Tu-182,
Tu-212, Tu-212LN(synonymous cell lines,
donor origin unknown)

T409

Human

Oral squamous cell
carcinoma

CVCL_1Q88

UM-SCC-1

Human

Oral squamous cell
carcinoma(recurrence)

CVCL_7707

Zhao et al, 2011

21868764

T-9(WI-38 derivative)

Human

Lung cells,
transformed normal
diploid fibroblasts

CVCL_M092

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Nelson-Rees et al,
19816451928

TCO-1

Human

Cervical carcinoma

CVCL_3179

TCS

Human

Cervical carcinoma

CVCL_8160

Yoshino et al, 2006

16643607

TDL-1

Human

CVCL_8428

P3JHR-1

Human

Lymphoma, Burkitt

CVCL_2676

TDL-2

Human

CVCL_8429

P3JHR-1

Human

Lymphoma, Burkitt

CVCL_2676

TDL-3

Human

CVCL_8430

RPMI 1788

Human

Lymphoblastoid cellline, normal donor

CVCL_2710

TDL-4

Human

CVCL_8431

Raji

Human

Lymphoma, Burkitt

CVCL_0511

CVCL_1762

TE-2, TE-3, TE-7, TE-12,
TE-13 (synonymous cell
Humanlines, donor origin
unknown)

Esophageal
squamous cell
carcinoma

CVCL_4463

TE-2, TE-3, TE-7, TE-12,
TE-13 (synonymous cell
Humanlines, donor origin
unknown)

Esophageal
squamous cell
carcinoma

CVCL_4455

TE-2, TE-3, TE-7, TE-12,
TE-13 (synonymous cell
Humanlines, donor origin
unknown)

Esophageal
squamous cell
carcinoma

Esophageal
squamous cell
carcinoma

Sarcoma
CVCL_1649(rhabdomyosarcoma)

Tonsillar lymphoidcells, non-neoplastic
Tonsillar lymphoidcells, non-neoplastic
Tonsillar lymphoidcells, non-neoplastic
Tonsillar lymphoidcells, non-neoplastic

Human

Esophageal
squamous cell
carcinoma

Human

Esophageal
squamous cell
carcinoma

Human

Esophageal
squamous cell
carcinoma

TE-3

Human

Esophageal
squamous cell
carcinoma

CVCL_9971

TE-2, TE-3, TE-7, TE-12,
TE-13 (synonymous cell
Humanlines, donor origin
unknown)

TE671

Human

Medulloblastoma

CVCL_1756

RD

TE-12

TE-13

TE-2

Human

CVCL_4455 /CVCL_9971 /CVCL_9972 /CVCL_1762 /CVCL_4463
CVCL_4455 /CVCL_9971 /CVCL_9972 /CVCL_1762 /CVCL_4463
CVCL_4455 /CVCL_9971 /CVCL_9972 /CVCL_1762 /CVCL_4463
CVCL_4455 /CVCL_9971 /CVCL_9972 /CVCL_1762 /CVCL 4463

Nelson-Rees et al,
1981
Nelson-Rees et al,
1981
Nelson-Rees et al,
1981
Nelson-Rees et al,
19816451928
6451928
6451928
6451928

Boonstra et al, 2007

17804709

Boonstra et al, 2007

17804709

Boonstra et al, 2007

17804709

Boonstra et al, 2007

17804709

Stratton et al, 1989;
Chen et al, 1989

2650908, 2739733

Page 20

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeTE671 Subline No.2

Medulloblastoma

Human

Sarcoma
CVCL_1649(rhabdomyosarcoma)

Human

CVCL_2731

RD

Esophageal
squamous cell
carcinoma

Contaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

Reference
PubMed ID

ECACC website,
ATCC FAQ #1063

No PMID

CVCL_4455 /CVCL_9971 /CVCL_9972 /CVCL_1762 /CVCL 4463

Boonstra et al, 2007

17804709

TE-7

Human

Esophageal
adenocarcinoma

CVCL_9972

TE-2, TE-3, TE-7, TE-12,
TE-13 (synonymous cell
Humanlines, donor origin
unknown)

TEC61

Human

Thyroid, endothelium

CVCL_7167

JEG3

Human

Choriocarcinoma

CVCL_0363

Patel et al, 2003
[retraction]

12388152

TI-1

Human

Leukemia, acute
myeloid, M2

CVCL_L806

K-562

Human

Leukemia, chronic
myeloid, blast crisis

CVCL_0004

Rush et al, 2002;
Drexler et al, 2003

11871388, 12592342

TK-1

Human

Glioblastoma

CVCL_B325

U-251 MG

Human

Glioblastoma

CVCL_0021

JCRB website

No PMID

TMH-1

Human

Thyroid, benign goitre CVCL_3219

IHH-4

Human

CVCL_2960

JCRB website

No PMID

TMM

Human

Leukemia, chronic
myeloid, blast crisis

CVCL_1894

Correct name, incorrectcell type

Human

None

Drexler et al, 2003

12592342

TSU-Pr1

Human

Prostate carcinoma

CVCL_4014

T-24

Human

CVCL_0554

van Bokhoven et al,
2001

11522622

Zhao et al, 2011

21868764

Zhao et al, 2011

21868764

Zhao et al, 2011

21868764

CVCL_1T17

T404, T406, Tu-138, Tu158LN, Tu-159, Tu-182,
Tu-212, Tu-212LN(synonymous cell lines,
donor origin unknown)

Human

Head and necksquamous cell
carcinoma

Human

Oropharyngeal
squamous cell
carcinoma

CVCL_4911

T404, T406, Tu-138, Tu158LN, Tu-159, Tu-182,
Tu-212, Tu-212LN(synonymous cell lines,
donor origin unknown)

Human

Head and necksquamous cell
carcinoma

Human

Oral squamous cell
carcinoma

CVCL_4912

UM-SCC-1

Human

Oral squamous cell
carcinoma(recurrence)

CVCL_7707

Zhao et al, 2011

21868764

Head and necksquamous cell
carcinoma

CVCL_1T20 /CVCL_1T19 /CVCL_4910 /CVCL_1T17 /CVCL_4911 /CVCL_4914 /CVCL_4915 /CVCL 1T18

Zhao et al, 2011

21868764

Human

Oropharyngeal
squamous cell
carcinoma

Tu-159Tu-167

Tu-182

CVCL_1T20 /CVCL_1T19 /CVCL_4910 /CVCL_1T17 /CVCL_4911 /CVCL_4914 /CVCL_4915 /CVCL 1T18
CVCL_1T20 /CVCL_1T19 /CVCL_4910 /CVCL_1T17 /CVCL_4911 /CVCL_4914 /CVCL_4915 /CVCL 1T18
CVCL_1T20 /CVCL_1T19 /CVCL_4910 /CVCL_1T17 /CVCL_4911 /CVCL_4914 /CVCL_4915 /CVCL 1T18

Head and necksquamous cell
carcinoma

Oral squamous cell
carcinoma

Tu-158LN

CVCL_4910

Bladder carcinoma

Human

Human

Tu-138

T404, T406, Tu-138, Tu158LN, Tu-159, Tu-182,
Tu-212, Tu-212LN(synonymous cell lines,
donor origin unknown)

Thyroid, papillary
thyroid carcinoma
EBV+ Blymphoblastoid cell
line

Human

Oropharyngeal
squamous cell
carcinoma

CVCL_4914

T404, T406, Tu-138, Tu158LN, Tu-159, Tu-182,
Tu-212, Tu-212LN(synonymous cell lines,
donor origin unknown)

Human

Page 21

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell Type

CVCL_4915

T404, T406, Tu-138, Tu158LN, Tu-159, Tu-182,
Tu-212, Tu-212LN(synonymous cell lines,
donor origin unknown)

Human

Head and necksquamous cell
carcinoma

CVCL_1T18

T404, T406, Tu-138, Tu158LN, Tu-159, Tu-182,
Tu-212, Tu-212LN(synonymous cell lines,
donor origin unknown)

Human

Head and necksquamous cell
carcinoma

Human

Hypopharyngeal
squamous cell
carcinoma

Tu-212LN

Human

Hypopharyngeal
squamous cell
carcinoma (lymph
node)

TuWi

Human

Kidney, Wilms' tumor CVCL_8275

HeLa

Human

Cervicaladenocarcinoma

U-118 MG

Human

Glioblastoma

CVCL_0633

U-138 MG

Human

UM-UC-2

Human

Bladder carcinoma

CVCL_8155

T-24

Human

UM-UC-3-GFP
Human(UM-UC-3 derivative)

Bladder carcinoma,
GFP-transfected

CVCL_1R40

UPES/CHuman

UPHHJA

Tu-212

Contaminating Cell
Line, Cellosaurus AC
CVCL_1T20 /CVCL_1T19 /CVCL_4910 /CVCL_1T17 /CVCL_4911 /CVCL_4914 /CVCL_4915 /CVCL 1T18
CVCL_1T20 /CVCL_1T19 /CVCL_4910 /CVCL_1T17 /CVCL_4911 /CVCL_4914 /CVCL_4915 /CVCL 1T18

Misidentification
Reported By

Reference
PubMed ID

Zhao et al, 2011

21868764

Zhao et al, 2011

21868764

CVCL_0030

Lavappa, 1978;
Nelson-Rees et al,
19816451928

Glioblastoma

CVCL_0020

ATCC website

No PMID

Bladder carcinoma

CVCL_0554

Chiong et al, 2009

19375735

Unknown, NOT UM-UC-3 Human

Unknown

None

Chiong et al, 2009

19375735

Brain, non-neoplastic
CVCL_1R55
astrocytes

UnknownRat

Unknown

None

Higgins et al, 2010

20951163

Human

Glioblastoma

CVCL_1R56

UnknownRat

Unknown

None

Higgins et al, 2010

20951163

UTMB-460

Human

B-cells

CVCL_8276

CCRF-CEM

Human

Leukemia, acutelymphoblastic, T cell

CVCL_0207

Drexler et al, 2003

12592342

VC312R

Human

Medulloblastoma

CVCL_1R57

Unknown

Mixture, human +mouse

Unknown

None

Higgins et al, 2010

20951163

VM-CUB-III

Human

Bladder carcinoma

CVCL_9830

VM-CUB-I

Human

Bladder carcinoma

CVCL_1786

Masters et al, 1986

3708594

WiDr

Human

Colon carcinoma

CVCL_2760

HT-29

Human

Colon carcinoma

CVCL_0320

Chen et al, 1987

3472642

CVCL_0030

CVCL_0030

WISH

Human

Amnion, normal cells

CVCL_1909

HeLa

Human

Cervicaladenocarcinoma

Wong-Kilbourne
derivative (WKD)

Human

Conjunctiva

CVCL_2764

HeLa

Human

Cervicaladenocarcinoma

WRL 68Human

Liver, embryonic cells CVCL_0581

HeLa

Human

WSU-ALCL

Human

Lymphoma,
anaplastic large cell

CVCL_A036

CCRF-CEM

Human

WSU-CLL

Human

Leukemia, chronic
lymphocytic

CVCL_A049

REH

Human

Cervicaladenocarcinoma
Leukemia, acutelymphoblastic, T cell
Leukemia, acutelymphoblastic, B cell
precursor

Gartler, 1967;
Lavappa, 1978;
Nelson-Rees et al,1981
Lavappa, 1978;
Nelson-Rees et al,
19814864103, 566722,
6451928566722, 6451928

CVCL_0030

ECACC website

No PMID

CVCL_0207

Drexler et al, 2003

12592342

CVCL_1650

Drexler et al, 2002;
Drexler et al, 2003

12200708, 12592342

Page 22

Version 8.0 Table 1
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Species
Line

Misidentified Cell
Contaminating Cell
Claimed Cell Type Line, Cellosaurus
Line
AC

Actual Species

Actual Cell Type

YAA

Human

Monocytes

CVCL_8466

U-937

Human

Lymphoma, histiocytic CVCL_0007

Drexler et al, 2003

12592342

YAP

Human

Monocytes

CVCL_8467

U-937

Human

Lymphoma, histiocytic CVCL_0007

Drexler et al, 2003

12592342

YJ

Human

Leukemia, chronic
myelomonocytic

CVCL_8931

HL-60

Human

Leukemia, acute
myeloid, M2

CVCL_0002

Drexler et al, 2003

12592342

YMB-1

Human

Breast carcinoma

CVCL_2814

ZR-75-1

Human

Breast carcinoma

CVCL_0588

Capes-Davis et al,
201323136038

YMB-1-E

Human

Breast carcinoma

CVCL_2815

ZR-75-1

Human

Breast carcinoma

CVCL_0588

Capes-Davis et al,
201323136038

Contaminating Cell
Line, Cellosaurus AC

Misidentification
Reported By

Reference
PubMed ID

Page 23

Version 8.0 Table 2
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)
The database of cross-contaminated or misidentified cell lines consists of two tables.

Table 1 (see previous pages) contains those cell lines where there is no authentic stock known to the list contributors. Table 2 (below) contains cell lines where some stocks have been shown to be misidentified, but where authentic
stocks are known to exist.
To help scientists obtain authentic stocks, an additional column in this table shows possible locations for authentic stock. If supplied by a cell line repository, the catalogue number is given in brackets. Repositories are listed in alphabetical order.

Table 2. Misidentified cell lines where authentic stock is known to exist
Misidentified Cell
Claimed Cell
Claimed Species
Line
Type

1483

Human

Oral squamous cell
carcinoma

Misidentified Cell
Contaminating Cell
Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell Authentic Stock
Line, Cellosaurus AC Location

CVCL_6980

Human

Oral squamous cell
carcinoma(recurrence)

CVCL_7707

Peter G. Sacks, NewYork University
Zhao et al, 2011
(originator)

21868764

T404, T406, Tu-138, Tu158LN, Tu-159, Tu-182,
Human
Tu-212, Tu-212LN(synonymous cell lines,
donor origin unknown)

Head and necksquamous cell
carcinoma

CVCL_1T20 /CVCL_1T19 /CVCL_4910 /CVCL_1T17 /CVCL_4911 /CVCL_4914 /CVCL_4915 /CVCL 1T18

Peter G. Sacks, NewYork University
Zhao et al, 2011
(originator)

21868764

UM-SCC-1

Misidentification
Reported By

Reference
PubMed ID

183

Human

Oropharyngeal
squamous cell
carcinoma

207 (EU-2)

Human

Leukemia, acutelymphoblastic, B cell CVCL_K034
precursor

REH and SUP-B2

Human

Leukemia, acute
CVCL_1650 /lymphoblastic, B cell
CVCL_A312
precursor

Amdur II (AMDURII) Human

Skin

CVCL_3643

LLC-PK1

Pig, Sus scrofa

Kidney, normal renal
CVCL_0391
cells

ATCC (CCL-124)

Milanesi et al, 2003;
14505435, 25877200
Yu et al, 2015

BG-1

Human

Ovarian carcinoma

CVCL_6570

MCF-7

Human

Breast carcinoma

CVCL_0031

University ofMontpellier, France

Korch et al, 2012; Liet al, 2014

BJA-B

Human

Bladder carcinoma

CVCL_5711

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

BT-20

Human

Breast carcinoma

CVCL_0178

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

Ca9-22

Human

Oral squamous cell
carcinoma

CVCL_1102

MSK-922

Human

Laryngeal squamous
CVCL_6989
cell carcinoma(recurrence)

COLO-720E

Human

Ovarian carcinoma

CVCL_1995

COLO704

Human

Uterineadenocarcinoma

CVCL_1994 /CVCL_AV69

D-17

Dog, Canisfamiliaris

Sarcoma(osteosarcoma)

CVCL_1916

MorescoDog, Canisfamiliaris

Sarcoma(osteosarcoma)

CVCL_L363

EB-3

Human

Lymphoma, Burkitt

CVCL_1185

Daudi

Human

Lymphoma, Burkitt

FU-RPNT-2

Human

Grey

Dog, Canisfamiliaris

Kidney, renal
primitive
neuroectodermaltumor
Sarcoma(osteosarcoma)

CVCL_6978

CVCL_4838

FU-RPNT-1

Human

Kidney, renal
primitive
neuroectodermal
tumor

CVCL_L354

Unknown

Human

Unknown

MacLeod et al, 1999;
10508494, 12592342
Drexler et al, 2003

22710073, 25321415

Nelson-Rees &1246601
Flandermeyer, 1976
ATCC (HTB-19)

Nelson-Rees et al,
19816451928JCRB (JCRB0625);
RIKEN (RCB1976)

Zhao et al, 2011

21868764

Korch et al, 2012;
ICLAC
correspondence

22710073

ATCC (CCL-183)

O'Donoghue et al,
2011

21908323

CVCL_0008

ATCC (CCL-85);
ECACC (90121003)

JCRB website

No PMID

CVCL_4837

RIKEN (RCB2078)

RIKEN website

No PMID

None

O'Donoghue et al,
2011

21908323

Page 24

Version 8.0 Table 2
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Cell
Claimed Species
Line
Type

Misidentified Cell
Contaminating Cell
Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell Authentic Stock
Line, Cellosaurus AC Location

HPB-ALL

Human

Leukemia, acute
CVCL_1820
lymphoblastic, T cell

JURKAT

Human

Leukemia, acute
CVCL_0065
lymphoblastic, T cell

J-82

Human

Bladder carcinoma

T-24

Human

Bladder carcinoma

CVCL_0554

JHH-1

Human

Liver, hepatocellular
CVCL_2785
carcinoma

Unknown

Mouse

Unknown

None

KARPAS-45

Human

Leukemia, acute
CVCL_1326
lymphoblastic, T cell

Unknown

Human

Unknown

None

KBM-3

Human

Leukemia, acute
myeloid, M4

HL-60

Human

Leukemia, acute
myeloid, M2

CVCL_0002

KE-37

Human

Leukemia, acute
CVCL_1327
lymphoblastic, T cell

CCRF-CEM

Human

Leukemia, acute
CVCL_0207
lymphoblastic, T cell

L-540

Human

Lymphoma, Hodgkin CVCL_1362

CCRF-CEM

Human

Leukemia, acute
CVCL_0207
lymphoblastic, T cell

MB-02

Human

CVCL_7075

HU-3

Human

MDA1986LN

Human

CVCL_AT80

UM-SCC-1

Human

MDA686LN

Human

CVCL_6984

UM-SCC-1

Human

Leukemia, acute
myeloid, M7
Oral squamous cell
carcinoma (lymph
node)
Oropharyngeal
squamous cell
carcinoma (lymph
node)

CVCL_0359

CVCL_A425

CVCL_6985

T404, T406, Tu-138, Tu158LN, Tu-159, Tu-182,
Human
Tu-212, Tu-212LN(synonymous cell lines,
donor origin unknown)

Leukemia, acute
myeloid, M7
Oral squamous cell
carcinoma(recurrence)

DSMZ (ACC-483);
RIKEN (RCB1935)

Misidentification
Reported By

Reference
PubMed ID

Drexler et al, 2003

12592342

Masters et al, 1986

3708594

JCRB (JCRB1062)

JCRB website

No PMID

DSMZ (ACC-105),
ECACC (06072602)

Drexler et al, 1999

10516762

Drexler et al, 2003

12592342

DSMZ (ACC-46)

Drexler et al, 1999

10516762

DSMZ (ACC-72)

Drexler et al, 2003

12592342

Drexler et al, 1999

10516762

CVCL_A619
CVCL_7707

Peter G. Sacks, New
Zhao et al, 2011
York University(originator)

21868764

Oral squamous cell
carcinoma(recurrence)

CVCL_7707

Peter G. Sacks, NewYork University
Zhao et al, 2011
(originator)

21868764

Head and necksquamous cell
carcinoma

CVCL_1T20 /CVCL_1T19 /CVCL_4910 /CVCL_1T17 /CVCL_4911 /CVCL_4914 /CVCL 4915 /Peter G. Sacks, NewYork University
Zhao et al, 2011
(originator)

21868764

MDA686TU

Human

Oropharyngeal
squamous cell
carcinoma

MKN-7

Human

Gastric carcinoma

CVCL_1417

Unknown

Human

Lymphoblastoid cells None

NCI-H929

Human

Myeloma

CVCL_1600

K-562

Human

Leukemia, chronic
myeloid, blast crisis

CVCL_0004

OCI-Ly3

Human

Lymphoma, nonHodgkin, diffuse
large B-cell

CVCL_8800

Unknown

Human

Unknown

CVCL_AT69

Parks

Dog, Canisfamiliaris

Melanoma

CVCL_DN32

Unknown

Human

Unknown

None

RMG-II

Human

Ovarian carcinoma

CVCL_2803

RMG-I

Human

Ovarian carcinoma

CVCL_1662

JCRB (JCRB0172.1) JCRB website

No PMID

RPMI-8402

Human

Leukemia, acute
CVCL_1667
lymphoblastic, T cell

Unknown

Human

Unknown

None

DSMZ (ACC-290)

Drexler et al, 1999

10516762

RT4

Human

Bladder carcinoma

HeLa

Human

Cervicaladenocarcinoma

CVCL_0030

ATCC (HTB-2);
DSMZ (ACC-412);
ECACC (91091914)

Nelson-Rees et al,
19816451928

CVCL_0036

JCRB (JCRB1025);
RIKEN (RCB0999)
ATCC (CRL-9068);
DSMZ (ACC-163);
ECACC (95050415)

DMSZ (ACC-761)

Suzuki and
Sekiguchi, 1999

No PMID (bookchapter)

MacLeod et al, 2013 23319659

Personal
communication, A.
BairochNo PMID

O'Donoghue et al,
2011

21908323

Page 25

Version 8.0 Table 2
Publication Date 1/12/2016

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Misidentified Cell
Claimed Cell
Claimed Species
Line
Type

Misidentified Cell
Contaminating Cell
Line, Cellosaurus
Line
AC

Actual Species

Actual Cell TypeContaminating Cell Authentic Stock
Line, Cellosaurus AC Location

Misidentification
Reported By

T3M-12

CVCL_4058

Human

Oral carcinoma

CVCL_8289

RIKEN (RCB2281)

RIKEN website

No PMID

CVCL_0021

ECACC (08061901)

Torsvik et al, 2014;
Timerman & Yeung,
2014; ECACC
website; ATCC
website24810477, 24333272

ATCC (CRL-1593.2);
DSMZ (ACC-5);
Drexler et al, 1999
ECACC (85011440);
JCRB (JCRB9021)

10516762

Johnen et al, 2013

23691160

Drexler et al, 2003

12592342

O'Donoghue et al,
2011

21908323

U-373 MG

Human

Lung carcinoma

Human

Glioblastoma

U-937

Human

Lymphoma,histiocytic

UROtsa

Human

UT-7

Human

YamadaDog, Canisfamiliaris

Bladder, nonmalignant urothelial
cells
Leukemia, acute
myeloid, M7
Sarcoma(osteosarcoma)

CVCL_2219

T3M-1

U-251 MG

Human

Glioblastoma

CVCL_0007

Unknown

Human

Unknown

None

CVCL_0571

T-24

Human

Bladder carcinoma

CVCL_0554

CVCL_2233

U-937

Human

Lymphoma,histiocytic

CVCL_0007

CVCL_FA16

Unknown

Mouse

Unknown

None

DSMZ (ACC-137)

Reference
PubMed ID

Page 26

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

Version 8.0 References
Publication Date 1/12/2016

Cell Bank Websites Cited
Name
ATCC:
American Type
Culture Collection

Websitehttp://www.atcc.org/

DSMZ:
Deutsche Sammlungvon Mikroorganismen http://www.dsmz.de/und Zellkulturen
GmbH
ECACC:
European Collection http://www.hpacultures.org.uk/collections/ecacc.jsp
of Cell Cultures
JCRB:
Japanese Collection
http://cellbank.nibio.go.jp/
of Research
Bioresources
RIKEN Bioresource
Center Cell Bank

http://www.brc.riken.go.jp/lab/cell/english/guide.shtml

References
PubMed ID
17254797
22570425
17804709

20075370
21253487

23907998

23136038
18196576

Reference
Azari S, Ahmadi N, Tehrani MJ, Shokri F. Profiling and authentication of human cell lines using short tandem repeat (STR) loci: Report from the
National Cell Bank of Iran. Biologicals 2007; 35: 195-202.
Bady P, Diserens AC, Castella V, Kalt S, Heinimann K, Hamou MF, Delorenzi M, Hegi ME. DNA fingerprinting of glioma cell lines andconsiderations on similarity measurements. Neuro Oncol 2012; 14: 701-11.
Boonstra JJ, van der Velden AW, Beerens EC, van Marion R, Morita-Fujimura Y, Matsui Y, Nishihira T, Tselepis C, Hainaut P, Lowe AW,
Beverloo BH, van Dekken H, Tilanus HW, Dinjens WN. Mistaken identity of widely used esophageal adenocarcinoma cell line TE-7. Cancer Res
2007; 67: 7996-8001.
Boonstra JJ, van Marion R, Beer DG, Lin L, Chaves P, Ribeiro C, Pereira AD, Roque L, Darnton SJ, Altorki NK, Schrump DS, Klimstra DS, Tang
LH, Eshleman JR, Alvarez H, Shimada Y, van Dekken H, Tilanus HW, Dinjens WN. Verification and unmasking of widely used humanesophageal adenocarcinoma cell lines. J Natl Cancer Inst 2010; 102: 271-4.
Brüderlein S, Sommer JB, Meltzer PS, Li S, Osada T, Ng D, Möller P, Alcorta DA, Kelley MJ. Molecular characterization of putative chordoma
cell lines. Sarcoma 2010; 2010: 630129.
Capes-Davis A, Alston-Roberts C, Kerrigan L, Reid YA, Barrett T, Burnett EC, Cooper JR, Freshney RI, Healy L, Kohara A, Korch C, Masters
JR, Nakamura Y, Nims RW, Storts DR, Dirks WG, Macleod RA, Drexler HG. Beware imposters: MA-1, a novel MALT lymphoma cell line, ismisidentified and corresponds to Pfeiffer, a diffuse large B-cell lymphoma cell line. Genes Chromosomes Cancer 2013; 52: 986-8.
Capes-Davis A, Reid YA, Kline MC, Storts DR, Strauss E, Dirks WG, Drexler HG, Macleod RA, Sykes G, Kohara A, Nakamura Y, Elmore E,
Nims RW, Alston-Roberts C, Barallon R, Los GV, Nardone RM, Price PJ, Steuer A, Thomson J, Masters JR, Kerrigan L. Match criteria forhuman cell line authentication: Where do we draw the line? Int J Cancer 2013; 132: 2510-9.
Chan SY, Choy KW, Tsao SW, Tao Q, Tang T, Chung GT, Lo KW. Authentication of nasopharyngeal carcinoma tumor lines. Int J Cancer 2008;
122: 2169-71.

8428522

Chen TR. Chromosome identity of human prostate cancer cell lines, PC-3 and PPC-1. Cytogenet Cell Genet 1993; 62: 183-4.

2739733

Chen TR, Dorotinsky C, Macy M, Hay R. Cell identity resolved. Nature 1989; 340: 106.

3472642
19375735
8105864
7905254
17786032
23472229
10614862

Chen TR, Drabkowski D, Hay RJ, Macy M, Peterson W Jr. WiDr is a derivative of another colon adenocarcinoma cell line, HT-29. Cancer
Genet Cytogenet 1987; 27: 125-34.
Chiong E, Dadbin A, Harris LD, Sabichi AL, Grossman HB. The Use of Short Tandem Repeat Profiling to Characterize Human Bladder Cancer
Cell Lines. J Urol 2009; 181: 2737-48.
Christensen B, Hansen C, Debiec-Rychter M, Kieler J, Ottensen S, Schmidt J. Identity of tumorigenic human urothelial cell lines and'spontaneously' transformed sublines. Br J Cancer 1993; 68: 879-84.
Christensen B, Hansen C, Kieler J, Schmidt J. Identity of non-malignant human urothelial cell lines classified as transformation grade I (TGrI)and II (TGrII). Anticancer Res 1993; 13: 2187-91.
Christgen M, Lehmann U. MDA-MB-435: the questionable use of a melanoma cell line as a model for human breast cancer is ongoing. Cancer
Biol Ther 2007; 6: 1355-7.
Dadon T, Ball D, Nelkin BD. Misidentification of Putative Medullary Thyroid Cancer Cell Lines RO-H85-1 and RO-D81-1. J Clin Endocrinol
Metab 2013; 98: 954-5.
Dirks WG, MacLeod RA, Drexler HG. ECV304 (endothelial) is really T24 (bladder carcinoma): cell line cross-contamination at source. In Vitro
Cell Dev Biol Anim 1999; 35: 558-9.

7682880

Drexler HG. Recent results on the biology of Hodgkin and Reed-Sternberg cells. II. Continuous cell lines. Leuk Lymphoma 1993; 9: 1-25.

10516762

Drexler HG, Dirks WG, MacLeod RA. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 1999; 13:
1601-7.

19344951

Drexler HG, Dirks WG, Macleod RA. Many are called MDS cell lines: One is chosen. Leuk Res 2009; 33: 1011-6.

12592342
11732505
12200708
10949990
20959409
12354931
18689700

Drexler HG, Dirks WG, Matsuo Y, MacLeod RA. False leukemia–lymphoma cell lines: an update on over 500 cell lines. Leukemia 2003; 17:416-26.
Drexler HG, MacLeod RA, Dirks WG. Cross-contamination: HS-Sultan is not a myeloma but a Burkitt lymphoma cell line. Blood 2001; 98: 34956.
Drexler HG, Quentmeier H, Dirks WG, Uphoff CC, MacLeod RA. DNA profiling and cytogenetic analysis of cell line WSU-CLL reveal crosscontamination with cell line REH (pre B-ALL). Leukemia 2002; 16: 1868-70.
Durkin AS, Cedrone E, Sykes G, Boles D, Reid YA. Utility of gender determination in cell line identity. In Vitro Cell Dev Biol Anim 2000; 36: 3447.
Ellis LM, Samuel S, Sceusi E. Varying Opinions on the Authenticity of a Human Midgut Carcinoid Cell Line - Letter. Clin Cancer Res 2010; 16:5365-6.
Ellison G, Klinowska T, Westwood RF, Docter E, French T, Fox JC. Further evidence to support the melanocytic origin of MDA-MB-435. Mol
Pathol 2002; 55: 294-9.
Folberg R, Kadkol SS, Frenkel S, Valyi-Nagy K, Jager MJ, Pe'er J, Maniotis AJ. Authenticating cell lines in ophthalmic research laboratories.
Invest Ophthalmol Vis Sci 2008; 49: 4697-701.

Page 27

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

PubMed ID

Version 8.0 References
Publication Date 1/12/2016

Reference

21406725

Frede S, Freitag P, Geuting L, Konietzny R, Fandrey J. Retraction: Oxygen-regulated expression of the erythropoietin gene in the human renal
cell line REPC. Blood 2011; 117: 4905-14.

4864103

Gartler, SM. Genetic markers as tracers in cell culture. Natl Cancer Inst Monogr 1967; 26: 167-95.

8220135

Gignac SM, Steube K, Schleithoff L, Janssen JW, MacLeod RA, Quentmeier H, Drexler HG. Multiparameter approach in the identification ofcross-contaminated leukemia cell lines. Leuk Lymphoma 1993; 10: 359-68.

4402510

Greene AE, Charney J, Nichols WW, Coriell LL. Species identity of insect cell lines. In Vitro 1972; 7: 313-22.

22236444
7192801
No PMID
22531032
20951163
1730567
23500642

23691160
24520087
11869090
22710073
23975727

Griewank KG, Yu X, Khalili J, Sozen MM, Stempke-Hale K, Bernatchez C, Wardell S, Bastian BC, Woodman SE. Genetic and molecularcharacterization of uveal melanoma cell lines. Pigment Cell Melanoma Res 2012; 25: 182-7.
Harris NL, Gang DL, Quay SC, Poppema S, Zamecnik PC, Nelson-Rees WA, O'Brien SJ. Contamination of Hodgkin's disease cell cultures.
Nature 1981; 289: 228-30.
Harvey GT, Sohi SS. Isozyme characterization of 28 cell lines from five insect species. Can J Zool 1985; 63: 2270-6.
Hashimoto Y, Zhang S, Zhang S, Chen YR, Blissard GW. Correction: BTI-Tnao38, a new cell line derived from Trichoplusia ni, is permissive for
AcMNPV infection and produces high levels of recombinant proteins. BMC Biotechnol 2012; 12: 12.
Higgins SC, Steingrimsdottir H, Pilkington GJ. Human, mouse or rat? Species authentication of glioma-derived cell cultures. J Neurosci
Methods 2010; 194: 139-43.
Honma M, Kataoka E, Ohnishi K, Ohno T, Takeuchi M, Nomura N, Mizusawa H. A new DNA profiling system for cell line identification for use incell banks in Japan. In Vitro Cell Dev Biol 1992; 28A: 24-8.
Jäger W, Horiguchi Y, Shah J, Hayashi T, Awrey S, Gust KM, Hadaschik BA, Matsui Y, Anderson S, Bell RH, Ettinger S, So AI, Gleave ME, Lee
IL, Dinney CP, Tachibana M, McConkey DJ, Black PC. Hiding in plain view: Genetic profiling reveals decades old cross-contamination of
bladder cancer cell line KU7 with HeLa. J Urol 2013; 190: 1404-9.
Johnen G, Rozynek P, von der Gathen Y, Bryk O, Zdrenka R, Johannes C, Weber DG, Igwilo-Okuefuna OB, Raiko I, Hippler J, Brüning T, Dopp
E. Cross-Contamination of a UROtsa Stock with T24 Cells - Molecular Comparison of Different Cell Lines and Stocks. PLoS One 2013; 8:
e64139.
Kniss DA, Summerfield TL. Discovery of HeLa Cell Contamination in HES Cells: Call for Cell Line Authentication in Reproductive Biology
Research. Reprod Sci 2014; 21: 1015-1019.
Kniss DA, Xie Y, Li Y, Kumar S, Linton EA, Cohen P, Fan-Havard P, Redman CW, Sargent IL. ED(27) trophoblast-like cells isolated from firsttrimester chorionic villi are genetically identical to HeLa cells yet exhibit a distinct phenotype. Placenta 2002; 23: 32-43.
Korch C, Spillman MA, Jackson TA, Jacobsen BM, Murphy SK, Lessey BA, Jordan VC, Bradford AP. DNA profiling analysis of endometrial andovarian cell lines reveals misidentification, redundancy and contamination. Gynecol Oncol 2012; 127: 241-8.
Krishnamoorthy RR, Clark AF, Daudt D, Vishwanatha JK, Yorio T. A Forensic Path to RGC-5 Cell Line Identification: Lessons Learned. Invest
Ophthalmol Vis Sci 2013; 54: 5712-9.

1250349

Lavappa KS, Macy ML, Shannon JE. Examination of ATCC stocks for HeLa marker chromosomes in human cell lines. Nature 1976; 259: 211-3.

566722

Lavappa KS. Survey of ATCC stocks of human cell lines for HeLa contamination. In Vitro 1978; 14: 469-475.

21938590
25321415
4027986
16504380
19372543
10508494
9447816
370859411416159
2903855

Lee LE, Bufalino MR, Christie AE, Frischer ME, Soin T, Tsui CK, Hanner RH, Smagghe G. Misidentification of OLGA-PH-J/92, believed to be
the only crustacean cell line. In Vitro Cell Dev Biol Anim 2011; 47: 665-674.
Li Y, Arao Y, Hall JM, Burkett S, Liu L, Gerrish K, Cavailles V, Korach KS. Research Resource: STR DNA Profile and Gene Expression
Comparisons of Human BG-1 Cells and a BG-1/MCF-7 Clonal Variant. Mol Endocrinol 2014; 28: 2072-81.
Lin CW, Lin JC, Prout GR Jr. Establishment and characterization of four human bladder tumor cell lines and sublines with different degrees ofmalignancy. Cancer Res 1985; 45: 5070-9.
Liscovitch M, Ravid D. A case study in misidentification of cancer cell lines: MCF-7/AdrR cells (re-designated NCI/ADR-RES) are derived fromOVCAR-8 human ovarian carcinoma cells. Cancer Lett 2007; 245: 350-2.
Lorenzi PL, Reinhold WC, Varma S, Hutchinson AA, Pommier Y, Chanock SJ, Weinstein JN. DNA fingerprinting of the NCI-60 cell line panel.
Mol Cancer Ther 2009; 8: 713-24.
MacLeod RA, Dirks WG, Matsuo Y, Kaufmann M, Milch H, Drexler HG. Widespread intraspecies cross-contamination of human tumor cell lines
arising at source. Int J Cancer 1999; 83: 555-63.
MacLeod RA, Dirks WG, Reid YA, Hay RJ, Drexler HG. Identity of original and late passage Dami megakaryocytes with HEL erythroleukemia
cells shown by combined cytogenetics and DNA fingerprinting. Leukemia 1997; 11: 2032-8.
Masters JR, Hepburn PJ, Walker L, Highman WJ, Trejdosiewicz LK, Povey S, Parkar M, Hill BT, Riddle PR, Franks LM. Tissue culture model oftransitional cell carcinoma: characterization of twenty-two human urothelial cell lines. Cancer Res 1986; 46: 3630-6.
Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani
A, Ward TH, Ayres KL, Debenham PG. Short tandem repeat profiling provides an international reference standard for human cell lines. Proc
Natl Acad Sci U S A 2001; 98: 8012-7.
Matsuba I, Lernmark A, Madsen O, Michelsen B, Nielsen JH, Scholler J, Vissing H, Welinder B, Tommerup N, Mikkelsen M, et al. Gene probes
to detect cross-culture contamination in hormone producing cell lines. In Vitro Cell Dev Biol 1988; 24: 1071-6.

15771911

Melcher R, Maisch S, Koehler S, Bauer M, Steinlein C, Schmid M, Kudlich T, Schauber J, Luehrs H, Menzel T, Scheppach W. SKY and genetic
fingerprinting reveal a cross-contamination of the putative normal colon epithelial cell line NCOL-1. Cancer Genet Cytogenet 2005; 158: 84-7.

14505435

Milanesi E, Ajmone-Marsan P, Bignotti E, Losio MN, Bernardi J, Chegdani F, Soncini M, Ferrari M. Molecular detection of cell line crosscontaminations using amplified fragment length polymorphism DNA fingerprinting technology. In Vitro Cell Dev Biol Anim 2003; 39: 124-30.

12740908

Moseley GW, Elliott J, Wright MD, Partridge LJ, Monk PN. Interspecies contamination of the KM3 cell line: implications for CD63 function inmelanoma metastasis. Int J Cancer 2003; 105: 613-6.

562836

Nelson-Rees WA. MA160 and HeLa cell lines. In Vitro 1977; 13: 525.

535908

Nelson-Rees WA. Prostatic acid phosphatase in cell lines MA 160, EB33 and HeLa cells. In Vitro 1979; 15: 935.

6451928

Nelson-Rees WA, Daniels DW, Flandermeyer RR. Cross-contamination of cells in culture. Science 1981; 212: 446-52.

1246601

Nelson-Rees WA, Flandermeyer RR. HeLa cultures defined. Science 1976; 191: 96-8.

7394535

Nelson-Rees WA, Flandermeyer RR, Daniels DW. T-1 cells are HeLa and not of normal human kidney origin. Science 1980; 209: 719-20.

21908323
9556756
83970276823318

O'Donoghue LE, Rivest JP, Duval DL. Polymerase chain reaction-based species verification and microsatellite analysis for canine cell line
validation. J Vet Diagn Invest 2011; 23: 780-785.
Ogura H, Fujii R, Hamano M, Kuzuya M, Nakajima H, Ohata R, Mori T. Detection of HeLa cell contamination--presence of human papillomavirus
18 DNA as HeLa marker in JTC-3, OG and OE cell lines. Jpn J Med Sci Biol 1997; 50: 161-7.
Ogura H,Yoshinouchi M,Kudo T,Imura M,Fujiwara T,Yabe Y. Human papillomavirus type-18 DNA in so-called HEP-2-cells. KB-cells and F1cells - further evidence that these cells are. HeLa-cell derivatives. Cell Mol Biol (Noisy-le-grand) 1993; 39: 463-7.
O'Toole CM, Povey S, Hepburn P, Franks LM. Identity of some human bladder cancer cell lines. Nature 1983; 301: 429-30.

Page 28

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

PubMed ID
11135436
12388152
7579375
19002917
19557180
17004106
19087340
15703300
11871388
11433418
18713817
20200241
15548687
22193509
46400722650908
24991015
22987579
No PMID

Version 8.0 References
Publication Date 1/12/2016

Reference
Pan Y, Lui WO, Nupponen N, Larsson C, Isola J, Visakorpi T, Bergerheim US, Kytölä S. 5q11, 8p11, and 10q22 are recurrent chromosomal
breakpoints in prostate cancer cell lines. Genes Chromosomes Cancer 2001; 30: 187-95.
Patel VA, Logan A, Watkinson JC, Uz-Zaman S, Sheppard MC, Ramsden JD, Eggo MC. Isolation and characterization of human thyroid
endothelial cells [retraction]. Am J Physiol Endocrinol Metab 2009; 297: E562.
Pellat-Deceunynk C, Amiot M, Bataille R, Van Riet I, Van Camp B, Omede P, Boccadoro M. Human myeloma cell lines as a tool for studying thebiology of multiple myeloma: a reappraisal 18 years after. Blood 1995; 86: 4001-2.
Perry GM, McDonald GJ, Ferguson MM, Ganassin RC, Bols NC. Characterization of rainbow trout cell lines using microsatellite DNA profiling.
Cytotechnology 2001; 37: 143-51.
Phuchareon J, Ohta Y, Woo JM, Eisele DW, Tetsu O. Genetic profiling reveals cross-contamination and misidentification of 6 adenoid cystic
carcinoma cell lines: ACC2, ACC3, ACCM, ACCNS, ACCS and CAC2. PLoS One 2009; 4: e6040.
Rae JM, Creighton CJ, Meck JM, Haddad BR, Johnson MD. MDA-MB-435 cells are derived from M14 melanoma cells--a loss for breast cancer,
but a boon for melanoma research. Breast Cancer Res Treat 2007; 104: 13-9.
Ribeiro FR, Meireles AM, Rocha AS, Teixeira MR. Conventional and molecular cytogenetics of human non-medullary thyroid carcinoma:characterization of eight cell line models and review of the literature on clinical samples. BMC Cancer 2008; 8: 371.
Roschke AV, Lababidi S, Tonon G, Gehlhaus KS, Bussey K, Weinstein JN, Kirsch IR. Karyotypic "state" as a potential determinant for
anticancer drug discovery. Proc Natl Acad Sci U S A 2005; 102: 2964-9.
Rush LJ, Heinonen K, Mrózek K, Wolf BJ, Abdel-Rahman M, Szymanska J, Peltomäki P, Kapadia F, Bloomfield CD, Caligiuri MA, Plass C.
Comprehensive cytogenetic and molecular genetic characterization of the TI-1 acute myeloid leukemia cell line reveals cross-contamination with
K-562 cell line. Blood 2002; 99: 1874-6.
Schmelz M, Cress AE, Barrera J, McDaniel KM, Davis TL, Fuchs L, Dalkin BL, Nagle RB. PEAZ-1: a new human prostate neoplastic epithelial
cell line [erratum]. Prostate. 2004; 61: 375.
Schweppe RE, Klopper JP, Korch C, Pugazhenthi U, Benezra M, Knauf JA, Fagin JA, Marlow LA, Copland JA, Smallridge RC, Haugen BR.
Deoxyribonucleic Acid profiling analysis of 40 human thyroid cancer cell lines reveals cross-contamination resulting in cell line redundancy andmisidentification. J Clin Endocrinol Metab 2008; 93: 4331-41.
Silversides DW, Music N, Jacques M, Webby R, Gagnon CA. Species origin investigation of the St. Jude porcine lung epithelial cell line (SJPL)
made available to researchers. J Virol 2010; 84: 5454-5.
Staege MS, Hutter C, Neumann I, Foja S, Hattenhorst UE, Hansen G, Afar D, Burdach SE. DNA microarrays reveal relationship of Ewing family
tumors to both endothelial and fetal neural crest-derived cells and define novel targets. Cancer Res 2004; 64: 8213-21.
Steube KG, Koelz AL, Uphoff CC, Drexler HG, Kluess J, Steinberg P. The necessity of identity assessment of animal intestinal cell lines: A casereport. Cytotechnology 2012; 64: 373-8.
Stoolmiller AC, Dorfman A, Sato GH. Rat origin of CHB cells. Science 1972; 178: 1308.
Stratton MR, Darling J, Pilkington GJ, Lantos PL, Reeves BR, Cooper CS. Characterization of the human cell line TE671. Carcinogenesis 1989;
10: 899-905.
Strong MJ, Baddoo M, Nanbo A, Xu M, Puetter A, Lin Z. Comprehensive high-throughput RNA sequencing analysis reveals contamination ofmultiple nasopharyngeal carcinoma cell lines with HeLa cell genomes. J Virol 2014; 88: 10696-704.
Stürzl M, Gaus D, Dirks WG, Ganem D, Jochmann R. Kaposi's sarcoma-derived cell line SLK is not of endothelial origin, but is a contaminantfrom a known renal carcinoma cell line. Int J Cancer 2013; 132: 1954-8.
Suzuki, T., Sekiguchi, M. (1999). Gastric cancer. In: Human Cell Culture Vol II, Masters JRW & Palsson, B, eds., Kluwer Academic Publishers,
Dordrecht, pp257-291.

15679051

Thompson EW, Waltham M, Ramus SJ, Hutchins AM, Armes JE, Campbell IG, Williams ED, Thompson PR, Rae JM, Johnson MD, Clarke R.
LCC15-MB cells are MDA-MB-435: a review of misidentified breast and prostate cell lines. Clin Exp Metastasis 2004; 21: 535-41.

24333272

Timerman D, Yeung CM. Identity confusion of glioma cell lines. Gene 2014; 536: 221-2.

22949886

Torsvik A, Røsland GV, Bjerkvig R. Spontaneous transformation of stem cells in vitro and the issue of cross-contamination. Int J Biol Sci 2012; 8:1051-2; discussion 1053-4.

24810477

Torsvik A, Stieber D, Enger PO, Golebiewska A, Molven A, Svendsen A, Westermark B, Niclou SP, Olsen TK, Chekenya Enger M, Bjerkvig R. U251 revisited: genetic drift and phenotypic consequences of long-term cultures of glioblastoma cells. Cancer Med 2014; 3: 812-24.

12453433
19443730
11522622
14518029
No PMID
316782312619888

Unger RE, Krump-Konvalinkova V, Peters K, Kirkpatrick CJ. In Vitro Expression of the Endothelial Phenotype: Comparative Study of Primary
Isolated Cells and Cell Lines, Including the Novel Cell Line HPMEC-ST1.6R. Microvasc Res 2002; 64: 384-97.
Van Bergen NJ, Wood JP, Chidlow G, Trounce IA, Casson RJ, Ju WK, Weinreb RN, Crowston JG. Recharacterization of the RGC-5 retinal
ganglion cell line. Invest Ophthalmol Vis Sci 2009; 50: 4267-72.
van Bokhoven A, Varella-Garcia M, Korch C, Miller GJ. TSU-Pr1 and JCA-1 cells are derivatives of T24 bladder carcinoma cells and are not of
prostatic origin. Cancer Res 2001; 61: 6340-4.
van Bokhoven A, Varella-Garcia M, Korch C, Johannes WU, Smith EE, Miller HL, Nordeen SK, Miller GJ, Lucia MS. Molecular characterizationof human prostate carcinoma cell lines. Prostate 2003; 57: 205-25.
van Bokhoven A. Models for prostate cancer: molecular characterization and critical appraisal of human prostate carcinoma cell lines. PhD
thesis 2004. University of Nijmegen, Nijmegen, The Netherlands.
van Helden PD, Wiid IJ, Albrecht CF, Theron E, Thornley AL, Hoal-van Helden EG. Cross-contamination of human esophageal squamous
carcinoma cell lines detected by DNA fingerprint analysis. Cancer Res 1988; 48: 5660-2.
van Pelt JF, Decorte R, Yap PS, Fevery J. Identification of HepG2 variant cell lines by short tandem repeat (STR) analysis. Mol Cell Biochem
2003; 243: 49-54.

17804723

van Staveren WC, Solís DW, Delys L, Duprez L, Andry G, Franc B, Thomas G, Libert F, Dumont JE, Detours V, Maenhaut C. Human thyroid
tumor cell lines derived from different tumor types present a common dedifferentiated phenotype. Cancer Res 2007; 67: 8113-20.

11433521

Varella-Garcia M,Boomer T,Miller GJ. Karyotypic similarity identified by multiplex-FISH relates four prostate adenocarcinoma cell lines: PC-3,
PPC-1, ALVA-31, and ALVA-41. Genes Chromosomes Cancer 2001; 31: 303-15.

21924324

Wang T, Rainey WE. Human adrenocortical carcinoma cell lines. Mol Cell Endocrinol 2012; 351: 58-65.

4043530

Whitaker AM, Hayward CJ. The characterization of three monkey kidney cell lines. Dev Biol Stand 1985; 60: 125-31.

6244232

Williams RD. Human urologic cancer cell lines. Invest Urol 1980; 17: 359-63.

8795574

Wilson AP, Dent M, Pejovic T, Hubbold L, Radford H. Characterisation of seven human ovarian tumour cell lines. Br J Cancer 1996; 74: 722-7.

19584161
16643607
25877200
24046071

Yip S, Miao J, Cahill DP, Iafrate AJ, Aldape K, Nutt CL, Louis DN. MSH6 mutations arise in glioblastomas during temozolomide therapy andmediate temozolomide resistance [erratum]. Clin Cancer Res 2009; 15: 4622-9.
Yoshino K, Iimura E, Saijo K, Iwase S, Fukami K, Ohno T, Obata Y, Nakamura Y. Essential role for gene profiling analysis in the authenticationof human cell lines. Hum Cell 2006; 19: 43-8.
Yu M, Selvaraj SK, Liang-Chu MM, Aghajani S, Busse M, Yuan J, Lee G, Peale F, Klijn C, Bourgon R, Kaminker JS, Neve RM. A resource forcell line authentication, annotation and quality control. Nature 2015; 520: 307-11.
Zhang P, Yang Y, Zweidler-McKay P, Hughes DP. Retraction: Critical role of notch signaling in osteosarcoma invasion and metastasis. Clin
Cancer Res 2013; 19: 5256-7.

Page 29

Database of Cross-Contaminated or Misidentified Cell Lines
International Cell Line Authentication Committee (ICLAC)

PubMed ID

21868764

Version 8.0 References
Publication Date 1/12/2016

Reference
Zhao M, Sano D, Pickering CR, Jasser SA, Henderson YC, Clayman GL, Sturgis EM, Ow TJ, Lotan R, Carey TE, Sacks PG, Grandis JR,
Sidransky D, Heldin NE, Myers JN. Assembly And Initial Characterization Of A Panel Of 85 Genomically Validated Cell Lines From Diverse
Head And Neck Tumor Sites. Clin Cancer Res 2011; 17: 7248.

Page 30