ANNUAL REPORTS
OF
INTERNATIONAL CENTER FOR BIOTECHNOLOGYOSAKA UNIVERSITY
VOL. 34, 2012
DIRECTOR/EDITORTAKUYA NIHIRAASSISTANT EDITOR

KAZUHITO FUJIYAMASHIGERU KITANIRYO MISAKIHIROSHI KINOSHITATAKAO OHASHISECRETARY

MEGUMI OKAMOTOFUMIKO TOMOMATSUYUKIKO SETOCHUNXIANG WANG

The Annual Report is published to record the activity of theInternational Center for Biotechnology (ICBiotech) and issued once in eachfiscal year. It contains scientific articles, progress reports, letters, andannouncement from the Center. This volume includes publications by theformer participants in UNESCO courses. The editor welcomes thesubmission of appropriate articles from all persons who are concerned withthe activity of the Center. All the contributions, however, will be
reviewed by editors before their acceptance. The scientific paper hereinshould be treated as personal communications and not treated as original
publications. The Annual Report is distributed upon request to theInternational Center for Biotechnology, Osaka University, 2-1 Yamadaoka,
Suita, Osaka 565-0871, Japan (e-mail: info_icbio@icb.osaka-u.ac.jp).

CONTENTS
Pleiotropic Control of Secondary Metabolism and Morphological Development
by KsbC, a Butyrolactone Autoregulator Receptor Homologue
in Kitasatospora setae
A. Aroonsri, S. Kitani, J. Hashimoto, I. Kosone, M. Izumikawa, M. Komatsu,
N. Fujita, Y. Takahashi, K. Shin-ya, H. Ikeda, and T. Nihira

1

Kitasetaline, a Novel β-Carboline Alkaloid from Kitasatospora setae
NBRC 14216T
A. Aroonsri, S. Kitani, H. Ikeda, and T. Nihira11

List of Publication: Takuya Nihira

11

Evolutionarily Conserved Glycan Signal to Degrade Aberrant
Brassinosteroid Receptors in Arabidopsis
Z. Honga, H. Kajiura, W. Su, H. Jin, A. Kimura, K. Fujiyama, and J. Li

12

Production of Different Glycosylation Variants of the Tumour-targetingmAb H10 in Nicotiana benthamiana: Influence on Expression Yield andAntibody Degradation
R. Lombardi, M. Donini, M. E. Villani, P. Brunetti, K. Fujiyama, H. Kajiura,
M. Paul, J. K. Ma, and E. Benvenuto

18

List of Publication: Kazuhito Fujiyama

19

CDC19 Encoding Pyruvate Kinase Is Important for High-temperature
Tolerance in Saccharomyces cerevisiae
S. Benjaphokee, P. Koedrith, C. Auesukaree, T. Asvarak, M. Sugiyama,
Y. Kaneko, C. Boonchird, and S. Harashima

20

Highly Efficient Bioethanol Production by a Saccharomyces cerevisiae
Strain with Multiple Stress Tolerance to High Temperature,
Acid and Ethanol
S. Benjaphokee, D. Hasegawa, D. Yokota, T. Asvarak, C. Auesukaree,
M. Sugiyama, Y. Kaneko, C. Boonchird, and S. Harashima

31

List of Publication: Satoshi Harashima32
Development of a Whole-cell Biocatalyst Co-expressing P450
Monooxygenase and Glucose Dehydrogenase
for Synthesis of Epoxyhexane
A. Siriphongphaew, P. Pisnupong, J. Wongkongkatep, P. Inprakhon,
A. S. Vangnai, K. Honda, H. Ohtake, J. Kato, J. Ogawa, S. Shimizu,
V. B. Urlacher, R. D. Schmid, and T. Pongtharangkul

i33

Metabolite Profiling of Soy Sauce Using Gas Chromatography
with Time-of-flight Mass Spectrometry and Analysis of Correlation
with Quantitative Descriptive Analysis
S. Yamamoto, T. Bamba, A. Sano, Y. Kodama, M. Imamura, A. Obata,and E. Fukusaki

44

Metabolomics-based Component Profiling of Hard andSemi-hard Natural Cheeses with Gas Chromatography/
Time-of-flight-mass Spectrometry, and Its Application
to Sensory Predictive Modeling
H. Ochi, H. Naito, K. Iwatsuki, T. Bamba, and E. Fukusaki

50

List of Publication: Eiichiro Fukusaki

51

Purification and Characterization of Phytase
from Klebsiella pneumoniae 9-3B
L. Escobin-Mopera, M. Ohtani, S. Sekiguchi, T. Sone, A. Abe,M. Tanaka, V. Meevootisom, and K. Asano

53

Are Uncultivated Bacteria Really Uncultivable?
I. D. Puspita, Y. Kamagata, M. Tanaka, K. Asano, and C. H. Nakatsu

59

List of Publication: Kozo Asano

59

Influence of a Mannan Binding Family 32 Carbohydrate Binding
Module on the Activity of the Appended Mannanase
K. Mizutani, V. O. Fernandes, S. Karita, A. S. Luís, M. Sakka, T. Kimura,
A. Jackson, X. Zhang, C. M. G. A. Fontes, H. J. Gilbert, and K. Sakka

60Characterization of Xyn30A and Axh43A of Bacillus licheniformis SVD1
Identified by Its Genomic Analysis
M. Sakka, S. Tachino, H. Katsuzaki, J. S. van Dyk, B. I. Pletschke,
T. Kimura, and K. Sakka

67

List of Publication: Kazuo Sakka67
Jomthonic Acid A, a Modified Amino Acidfrom a Soil-derived Streptomyces
Y. Igarashi, L. Yu, M. Ikeda, T. Oikawa, S. Kitani, T. Nihira,B. Bayanmunkh, and W. Panbangred

68

Prajinamide, a New Modified Peptide from a Soil-derived Streptomyces
Y. Igarashi, Y. Tanaka, M. Ikeda, T. Oikawa, S. Kitani, T. Nihira,P. Mongkol, M. Janhom, and W. Panbangred

73

List of Publication: Yasuhiro Igarashi

75

ii

Continuous Butanol Fermentation from Xylose with High Cell Density
by Cell Recycling System
J. Zheng, Y. Tashiro, T. Yoshida, M. Gao, Q. Wang, and K. Sonomoto

76

Identification of the Genes Involved in the Secretion and Self-immunityof Lacticin Q, an Unmodified Leaderless Bacteriocinfrom Lactococcus lactis QU 5
S. Iwatani, F. Yoneyama, S. Miyashita, T. Zendo, J. Nakayama,
and K. Sonomoto82

List of Publication: Kenji Sonomoto83
The 4-tert-Butylphenol-Utilizing Bacterium Sphingobium f uliginis
OMI Can Degrade Bisphenols via Phenolic Ring Hydroxylationand Meta-Cleavage Pathway
Y. Ogata, S. Goda, T. Toyama, K. Sei, and M. Ike85

Effective Selenium Volatilization under Aerobic Conditions and Recovery
from the Aqueous Phase by Pseudomonas stutzeri NT-I
T. Kagami, T. Narita, M. Kuroda, E. Notaguchi, M. Yamashita, K. Sei,
S. Soda, and M. Ike92

List of Publication: Michihiko Ike93
Enhanced Paclitaxel Production by Addition of Water-Soluble
5-Aminolevulinic Acid and In Situ Extraction with Laury Alcohol
in A Suspension Callus Culture
K. Taura, S. Yamamoto, S. Hayashi, and S. Shioya

95

Regular Ingestion of Cinnamomi Cortex Pulveratus Offers Gastroprotective
Activity in MiceJ. M. Tankam, Y. Sawada, and M. Ito

101

Sedative Effect of Vapor Inhalation of Essential Oilfrom Heracleum afghanicum Kitamura Seeds
A. G. Karimi and M. Ito

108

Complete Genome Sequence of Bradyrhizobium sp. S23321:
Insights into Symbiosis Evolution in Soil Oligotrophs
T. Okubo, T. Tsukui, H. Maita, S. Okamoto, K. Oshima, T. Fujisawa, A. Saito,
H. Futamata, R. Hattori, Y. Shimomura, S. Haruta, S. Morimoto, Y. Wang,
Y. Sakai, M. Hattori, S. Aizawa, K. V. P. Nagashima, S. Masuda, T. Hattori,
A. Yamashita, Z. Bao, M. Hayatsu, H. Kajiya-Kanegae, I. Yoshinaga,
K. Sakamoto, K. Toyota, M. Nakao, M. Kohara, M. Anda, R. Niwa,
J.-H. Park, R. Sameshima-Saito, S. Tokuda, Su. Yamamoto,Syu. Yamamoto, T. Yokoyama, T. Akutsu, Y. Nakamura, Y. Nakahira-Yanaka,iii

Y. Takada Hoshino, H. Hirakawa, H. Mitsui, K. Terasawa, M. Itakura,
S. Sato, W. Ikeda-Ohtsubo, N. Sakakura, E. Kaminuma, and K. Minamisawa

109

The Type III Secretion System of Bradyrhizobium japonicum USDA122Mediates Symbiotic Incompatibility with Rj2 Soybean
T. Tsukui, S. Eda, T. Kaneko, S. Sato, S. Okazaki, K. Kakizaki-Chiba,
M. Itakura, H. Mitsui, A. Yamashita, K. Terasawa, and K. Minamisawa

119

List of Publication: Kiwamu Minamisawa119
Biochemical Characterization of Pantoate Kinase, a Novel Enzyme Necessary
for Coenzyme A Biosynthesis in the Archaea
H. Tomita, Y. Yokooji, T. Ishibashi, T. Imanaka, and H. Atomi

120

A Detailed Biochemical Characterization of Phosphopantothenate Synthetase,a Novel Enzyme Involved in Coenzyme A Biosynthesis in the Archaea
T. Ishibashi, H. Tomita, Y. Yokooji, T. Morikita, B. Watanabe, J. Hiratake,
A. Kishimoto, A. Kita, K. Miki, T. Imanaka, and H. Atomi

130

List of Publication: Haruyuki Atomi131
Requirement of Ca2+ Ions for the Hyperthermostability of Tk-Subtilisin
from Thermococcus kodakarensis
R. Uehara, Y. Takeuchi, S. Tanaka, K. Takano, Y. Koga, and S. Kanaya

133

Structure and Characterization of RNase H3 from Aquifex aeolicus
N. Jongruja, D.-J. You, C. Angkawidjaja, E. Kanaya, Y. Koga,
and S. Kanaya

143

List of Publication: Shigenori Kanaya144
Network Formation through Active Migration of Human Vascular
Eendothelial Cells in a Multilayered Skeletal Myoblast Sheet
E. Nagamori, T. X. Ngo, Y. Takezawa, A. Saito, Y. Sawa, T. Shimizu,
T. Okano, M. Taya, and M. Kino-oka

145

Modulation of Chondrocyte Migration and Aggregation
by Insulin-like Growth Factor-1 in Cultured Cartilage
M. M. Nadzir, M. Kino-oka, K. Sugawara, and M. Taya

152

List of Publication: Masahiro Kino-oka

152

Metschnikowia saccharicola sp. nov. and Metschnikowia lopburiensis sp. nov.,
Two Novel Yeast Species Isolated from Phylloplane in Thailand
R. Kaewwichian, W. Yongmanitchai, H. Kawasaki, and S. Limtong

153

iv

Bioethanol Production from Sugar Cane Syrup by Thermo-tolerant Yeast,
Kluyveromyces marxianus DMKU3-1042, Using Fed-batch andRepeated-batch Fermentation in a Nonsterile System
P. Pimpakan, W. Yongmanitchai, and S. Limtong162

Comparative Metagenomic Analysis of Microcosm Structures andLignocellulolytic Enzyme Systems of
Symbiotic Biomass-degrading Consortia
S. Wongwilaiwalin, T. Laothanachareon, W. Mhuantong,
S. Tangphatsornruang, L. Eurwilaichitr, Y. Igarashi, and V. Champreda

163

Enhancement of Biogas Production from Swine Manure
by a Lignocellulolytic Microbial Consortium
S. Tuesorn, S. Wongwilaiwalin, V. Champreda, M. Leethochawalit,
A. Nopharatana, S. Techkarnjanaruk, and P. Chaiprasert

177

Bioproduction of Vanillin Using an Organic Solvent-tolerant
Brevibacillus agri 13
N. Wangrangsimagul, K. Klinsakul, A. S. Vangnai, J. Wongkongkatep,
P. Inprakhon, K. Honda, H. Ohtake, J. Kato, and T. Pongtharangkul

178

List of Publication: Thunyarat Pongtharangkul178
Heterologous Protein Expression in Pichia thermomethanolica BCC16875,
a Thermotolerant Methylotrophic Yeast and Characterization of
N-linked Glycosylation in Secreted Protein
S. Tanapongpipat, P. Promdonkoy, T. Watanabe, W. Tirasophon,
N. Roongsawang, Y. Chiba, and L. Eurwilaichitr

179

High-level Production of Thermotolerant β-xylosidase of Aspergillus sp.
BCC125 in Pichia pastoris: Characterization and Its Applicationin Ethanol Production
S. Wongwisansri, P. Promdonkoy, P. Matetaviparee, N. Roongsawang,
L. Eurwilaichitr, and S. Tanapongpipat

187

List of Publication: Sutipa Tanapongpipat187
Dimerized Glycosaminoglycan Chains Increase FGF Signaling
during Zebrafish Development
T. K. N. Nguyen, V. M. Tran, V. Sorna, I. Eriksson, A. Kojima, M. Koketsu,
D. Loganathan, L. Kjellén, R. I. Dorsky, C.-B. Chien, and B. Kuberanv

188

PUBLICATIONS by Former Participants in
UNESCO International Post-Graduate University Course in Microbiology (UM),UNESCO Postgraduate Inter-University Course in Biotechnology (UB),and UNESCO Training Project supported by ODA Grants for
UNESCO Activities, MEXT (UO)
Culture Filtrate of Root Endophytic Fungus Piriformospora indica Promotes
the Growth and Lignan Production of Linum album Hairy Root Cultures
V. Kumar, G. Rajauria, V. Sahai, and V. S. Bisaria

189

Responses of Cajanus cajan and Rhizospheric N-cycling Communities
to Bioinoculants
R. Gupta, D. Bru, V. S. Bisaria, L. Philippot, and S. Sharma

196

List of Publication: V. S. Bisaria196
Biogenic Amines in Some Natural and Processed Cheeses Soldin Laguna Province, Philippines
Ma. J. M. Vallejos, L. J. Pham, and V. L. Barraquio

197

Foaming Properties at Different Fat Levels and Age of Milk
G. V. Gamboa and V. L. Barraquio202

Construction of Bordetella pertussis Strains with Enhanced Production of
Genetically-inactivated Pertussis Toxin and Pertactin
by Unmarked Allelic Exchange
W. Buasri, A. Impoolsup, C. Boonchird, A. Luengchaichawange, P. Prompiboon,
J. Petre, and W. Panbangred

203

Large Crystal Toxin Formation in Chromosomally Engineered
Bacillus thuringiensis subsp. aizawai Due to σE Accumulation
W. Buasria and W. Panbangred

219

List of Publication: Watanalai Panbangred220
Tyrosine Phosphorylation of Botulinum Neurotoxin Protease Domains
S. Toth, E. E. Brueggmann, G. A. Oyler, L. A. Smith, H. B. Hines,
and S. A. Ahmed

221

Peptide Inhibitors of Botulinum Neurotoxin Serotype A: Design, Inhibition,
Cocrystal Structures, Structure-activity Relationship andPharmacophore Modeling
G. Kumar, D. Kumaran, S. A. Ahmed, and S. Swaminathan

233

List of Publication: Syed Ashrafuddin Ahmed233vi

α1-3/4 Fucosylation at Asn 241 of β-haptoglobin Is a Novel Marker
for Colon Cancer: A Combinatorial Approach for Developmentof Glycan Biomarkers
S.-Y. Park, S.-H. Lee, N. Kawasaki, S. Itoh, K. Kang, S. H. Ryu, N. Hashii,
J.-M. Kim, J.-Y. Kim, and J. H. Kim

234

Globoside Promotes Activation of ERK by Interaction
with the Epidermal Growth Factor Receptor
S.-Y. Park, C.-Y. Kwak, J. A. Shayman, and J. H. Kim

245

N-Glycoform Diversity of Cellobiohydrolase I from Penicillium decumbens
and Synergism of Nonhydrolytic Glycoform in Cellulose Degradation
L. Gao, F. Gao, L. Wang, C. Geng, L. Chi, J. Zhao, and Y. Qu

246

Genomic and Secretomic Analyses Reveal Unique Featuresof the Lignocellulolytic Enzyme System of Penicillium decumbens
G. Liu, L. Zhang, X. Wei, G. Zou, Y. Qin, L. Ma, J. Li, H. Zheng, S. Wang,
C. Wang, L. Xun, G.-P. Zhao, Z. Zhou, and Y. Qu

256

Lactic Acid Bacteria in PhilippineTraditional Fermented Foods
C. G. B. Banaay, M. P. Balolong, and F. B. Elegado

257

Postcommercialization Monitoring of the Long-Term Impact of Bt Cornon Non-Target Arthropod Communities in Commercial Farms
and Adjacent Riparian Areas in the Philippines
E. P. Alcantara

275

Allelic and Genotype Frequencies of Catechol-O-methyltransferase (Val158Met)
and CYP2D6*10 (Pro34Ser) Single Nucleotide Polymorphisms in the Philippines
M. O. Baclig, R. Z. Predicala, C. A. Mapua, J. P. Lozano-Kühne,M. L. G. Daroy, F. F. Natividad, and F. O. Javier

284

Prevalence of Dengue Infection and Accuracy of the Rapid Dengue-NS1
Antigen Strip Test as a Screening Tool for Dengue Infection
among Patients Presenting with Fever at the Emergency Roomof St. Luke’s Medical Center (A Prospective Study)
A. K. A. Ramos, F. J. C. Mesa-Gaerlan, P. F. Quilala, C. A. Mapua,
L.-A. C. Suarez, H. K. M. Labayo, M. L. G. Daroy, R. R. Matias,
and F. F. Natividad

291

Genetic Diversity, Symbiotic Evolution, and Proposed Infection Processof Bradyrhizobium Strains Isolated from Root Nodulesof Aeschynomene americana L. in Thailand
R. Noisangiam, K. Teamtisong, P. Tittabutr, N. Boonkerd, T. Uchiumi,
K. Minamisawa, and N. Teaumroong

292

vii

List of Publication: Neung Teaumroong307
High Level Lipid Production by a Novel Inulinase-producing Yeast
Pichia guilliermondii Pcla22
G.-Y. Wang, Z. Chi, B. Song, Z.-P. Wang, and Z.-M. Chi

308

Disruption of the Gene Encoding β-1, 3-Glucanase in Marine-Derived
Williopsis saturnus WC91-2 Enhances its Killer Toxin Activity
J.-L. Xu, X. Zhang, H.-Y. Sun, and Z.-M. Chi

314

List of Publication: Zhen-Ming Chi315

Production and Accumulation of 4-O-Methyla-d-Glucuronosyl-Xylotriose
by Growing Culture of Thermophilic Anoxybacillus sp. Strain JT-12
J. Thitikorn-amorn, K. L. Kyu, K. Sakka, and K. Ratanakhanokchai

317

Improved Purity and Immunostimulatory Activity of β-(1→3)(1→6)-Glucan
from Pleurotus sajor-caju Using Cell Wall-Degrading Enzymes
S. Satitmanwiwat, K. Ratanakhanokchai, N. Laohakunjit, L. K. Chao,
S.-T. Chen, P. Pason, C. Tachaapaikoon, and K. L. Kyu

327

Engineering a Disulfide Bond in the Lid Hinge Region of Rhizopus chinensis
Lipase: Increased Thermostability and Altered Acyl Chain Length Specificity
X.-W. Yu, N.-J. Tan, R. Xiao, and Y. Xu

328

Enhanced Thermostability of a Rhizopus chinensis Lipase by In Vivo
Recombination in Pichia pastoris
X.-W. Yu, R. Wang, M. Zhang, Y. Xu, and R. Xiao

335

List of Publication: Yan Xu336
Production and Characterization of a Monoclonal Antibody
against Enrofloxacin
M. Chusri, P. Wongphanit, T. Palaga, S. Puthong, S. Sooksai,
and K. Komolpis

337

Direct Fermentation of l(+)-lactic Acid from Cassava Pulp
by Solid State Culture of Rhizopus oryzae
P. Phrueksawan, S. Kulpreecha, S. Sooksai, and N. Thongchul

344

Genomic Analysis of Pseudomonas putida: Genes
in a Genome Island Are Crucial for Nicotine Degradation
H. Tang, Y. Yao, L. Wang, H. Yu, Y. Ren, G. Wu, and P. Xu

345

TK1299, a Highly Thermostable NAD(P)H Oxidase from Thermococcus
kodakaraensis Exhibiting Higher Enzymatic Activity with NADPH

viii

M. A. Nisar, N. Rashid, Q. Bashir, Q. A. A. Gardner, M. H. Shafiq,
and M. Akhtar

353

Cloning, Characterization and Molecular Docking
of a Highly Thermostable β-1,4-glucosidase from Thermotoga petrophila
I. U. Haq, M. A. Khan, B. Muneer, Z. Hussain, S. Afzal, S. Majeed,
N. Rashid, M. M. Javed, and I. Ahmad

359

List of Publication: Naeem Rashid

359

High Level Production of 5-Aminolevulinic Acidby Propionibacterium acidipropionici Grown in a Low-cost Medium
R. Sonhom, C. Thepsithar, and B. Jongsareejit

360

Purification and Characterization of Methyl Parathion Hydrolasefrom Burkholderia cepacia Capableof Dgrading Organophosphate Insecticides
A. Ekkhunnatham, B. Jongsareejit, W. Yamkunthong, and J. Wichitwechkarn

366

Fate of Fluoroquinolone Antibiotics in Vietnamese Coastal Wetland
Ecosystem
T. T. T. Hoang, L. T. C. Tu, N. P. Le, Q. P. Dao, and P. H. Trinh

367

A Preliminary Study on the Phytoremediationof Antibiotic Contaminated Sediment
T. T. T. Hoang, L. T. C. Tu, N. P. Le, and Q. P. Dao

377

Structure-Based Engineering of Methionine Residues
in the Catalytic Cores of Alkaline Amylase from Alkalimonas amylolytica
for Improved Oxidative Stability
H. Yang, L. Liu, M. Wang, J. Li, N. S. Wang, G. Du, and J. Chen

378

Metabolic Engineering of Escherichia coli BL21 for Biosynthesis
of Heparosan, a Bioengineered Heparin Precursor
C. Zhang, L. Liu, L. Teng, J. Chen, J. Liu, J. Li, G. Du, and J. Chen386

List of Publication: Jian Chen387
Association of Naturally Acquired IgG Antibodies against
Plasmodium falciparum Serine Repeat Antigen-5
with Reduced Placental Parasitemia and Normal Birth Weightin Pregnant Ugandan Women: A Pilot Study
T. J. Owalla, N. M. Q. Palacpac, H. Shirai, T. Horii, and T. G. EgwangWithin-population Genetic Diversity of Plasmodium falciparum
Vaccine Candidate Antigens Reveals Geographic Distance

ix

390

from a Central Sub-Saharan African Origin
K. Tanabe, T. Mita, N. M. Palacpac, N. Arisue, T. Tougan, S. Kawai,T. Jombart, F. Kobayashi, and T. Horii

393

List of Publication: Nirianne Marie Q. Palacpac393
Degradation of Polycyclic Aromatic Hydrocarbons by Newly Isolated
Curvularia sp. F18, Lentinus sp. S5, and Phanerochaete sp. T20
K. Juckpecha, O. Pinyakonga, and P. Rerngsamrana

394

Application of a New Red Carotenoid Pigment-producing Bacterium,
Enterobacter sp. P41, as Feed Supplement for Chicken
S. Tanskul1, S. Khoonchumnan, S. Watanasit, and K. Oda404

A New Cellulose-producing Bacterium, Rhodococcus sp. MI 2:
Screening and Optimization of Culture Conditions
S. Tanskul, K. Amornthatree, and N. Jaturonlak

410

Recruitment of TRF2 to Laser-induced DNA Damage Sites
N. Huda, S. Abe, L. Gu, M. S. Mendonca, S. Mohanty, and D. Gilley

411

Telomere Fusions in Early Human Breast Carcinoma
H. Tanaka, S. Abe, N. Huda, L. Tu, M. J. Beam, B. Grimes, and D. Gilley

417

Cloning, Expression, Characterization, and Biocatalytic Investigationof a Novel Bacilli Thermostable Type I Pullulanasefrom Bacillus sp. CICIM 263
Y. Li, L. Zhang, D. Niu, Z. Wang, and G. Shi418

Production of l-Phenylalanine from Glucose by Metabolic Engineeringof Wild Type Escherichia coli W3110
S.-P. Liu, M.-R. Xiao, L. Zhang, J. Xu, Z.-Y. Ding, Z.-H. Gu, and G.-Y. Shi427

List of Publication: Guiyang Shi427
Estrogen Receptor α/β-Cofactor Motif Interactions; Interplay of
Tyrosine 537/488 Phosphorylation and LXXLL Motifs
H. D. Nguyen, T. T. P. Phan, M. Carraz, and L. Brunsveld

428

Proline Primed Helix Length as a Modulatoof the Nuclear Receptor-Coactivator Interaction
S. Fuchs, H. D. Nguyen, T. T. P. Phan, M. F. Burton, L. Nieto,I. J. de Vries-van Leeuwen, A. Schmidt, M. Goodarzifard,
S. M. Agten, R. Rose, C. Ottmann, L.-G. Milroy, and L. Brunsveld

436

List of Publication: Hoang D. Nguyen436xChanges in Sequences of Core Region, Interferon Sensitivity-determining
Region and Interferon and Ribavirin Resistance-determining Regionof Hepatitis C Virus Genotype 1 during Interferon-alphaand Ribavirin Therapy, and Efficacy of Retreatment
R. Kozuka, M. Enomoto, H. Hai, T. Ogawa, M. Nakaya, A. Hagihara,
H. Fujii, S. Kobayashi, S. Iwai, H. Morikawa, A. Tamori, and N. Kawada

437

A Pregnant Woman with Acute Hepatitis B in Whom Vertical Transmission
Was Prevented by Tenofovir Disoproxil Fumarate
M. Tooyama, A. Tamori, A. Nakano, Hoang Hai, L. T. T. Thuy, M. Enomoto,and N. Kawada

448

Genome-Wide Sequence Characterization and Expression Analysis
of Major Intrinsic Proteins in Soybean (Glycine max L.)
D. Y. Zhang, Z. Ali, C. B. Wang, L. Xu, J. X. Yi, Z. L. Xu, X. Q. Liu, X. L. He,
Y. H. Huang, I. A. Khan, R. M. Trethowan, and H. X. Ma

449

Uncovering the Salt Response of Soybean by Unraveling Its Wild
and Cultivated Functional Genomes Using Tag Sequencing
Z. Ali, D. Y. Zhang, Z. L. Xu, L. Xu, J. X. Yi, X. L. He,
Y. H. Huang, X. Q. Liu, A. A. Khan, R. M. Trethowan, and H. X. Ma

462

Crystal Structure of Metagenome-derived LC11-RNase H1
in Complex with RNA/DNA Hybrid
T.-N. Nguyen, D.-J. You, H. Matsumoto, E. Kanaya, Y. Koga, and S. Kanaya

463

Crystal Structure of Metagenome-derived LC9-RNase H1
with Atypical DEDN Active Site Motif
T.-N. Nguyen, D.-J. You, E. Kanaya, Y. Koga, and S. Kanaya

474

Activities of International Center for Biotechnology for 2012

475

Author Index

495

xi

ACTIVITIES OF ICBiotech

ACTIVITIES OF INTERNATIONAL CENTER FOR
BIOTECHNOLOGY FOR FY2012
The International Center for Biotechnology (ICBiotech) was founded in April 1995 as anindependent institute in Osaka University with a mission to pursue academic advancement andcollaborative research in biotechnology. ICBiotech has its origin from the “International Centerof Cooperative Research Center in Microbial Engineering Japan (ICME)” which wasestablished in the Faculty of Engineering, Osaka University in April 1978, through renaming
to “International Center of Cooperative Research in Biotechnology (ICBiotech)” in April 1985
with the recognition of the wide acceptance and success of ICME’s activities and achievements.
ICBiotech is dedicated to promote international cooperation among Asian countries in the
aspects of research and educational advancement in the field of Biotechnology by propelling
academic interactions in Asian countries, and is committed to industrial biotechnology studies
by means of microbial engineering and related sciences, focusing on the sustainable utilizationof abundant natural resources in Southeast Asian countries.
ICBiotech serves as the seat of education and research in Asia, with the support of the
Ministry of Education, Culture, Sports, Science and Technology (Monbu-kagaku-sho, MEXT),the Japan Society for the Promotion of Science (JSPS), the Japan Science and Technology
Agency (JST), the Japan Student Service Organization (JASSO), and the auspices of the United
Nations Educational, Scientific and Cultural Organization (UNESCO), with the cooperation of
Department of Biotechnology, Graduate School of Engineering, Osaka University, andresearchers from prestigious universities nationwide and abroad.
In 2002 Mahidol University-Osaka University Collaborative Research Center for
Bioscience and Biotechnology: MU-OU:CRC was set up at Faculty of Science, Mahidol
University as a collaborative research center to accomplish multidisciplinary research in thefield of Bioscience and Biotechnology.
The activities of the ICBiotech include:
1. Research and Education
The main area of research is industrial biotechnology rooted in microbial engineering,whilst centering on the sustainable use of agricultural and forest resources in bioresourcerich countries such as those in Southeast Asia. Research is underway in the field of cellengineering with the objectives of analyzing the cellular functions of bacteria, fungi andplants, and developing and using functions of these cells for management and rational useof biological resources that exist on our planet. ICBiotech covers three areas of
Biotechnology:
1) Discovery of new functions from biological resources.
2) Bio-conversion and process engineering of biological resources.
3) Conservation of biological resources.
For Post-Graduate Course, ICBiotech is involved in the education activities of Department of Biotechnology, Graduate School of Engineering as collaborating laboratories andcurrently covers several fields of biotechnology in education and research: Molecular
Microbiology laboratory chaired by Prof. Takuya Nihira and Applied Microbiology
laboratory chaired by Prof. Kazuhito Fujiyama.

476

2. Participating in FrontierLab@OsakaU ’Scientific Empowerment Program for International
Students’ which was created for international students to conduct thermatic studies andachieve results under the guidance of supervisors while acquiring skills necessary forcontinuing research in one of Osaka University’s internationally renowned science andtechnology laboratories for a period of up to 12 months.
3. Acting as collaborating laboratories with Department of Biotechnology, Graduate Schoolof Engineering, Osaka University for ‘International Program of Frontier Biotechnology’, afive-year program for master’s and doctor’s degrees. The aim of this program is to expose
graduate students (privately financed as well as the Japanese Government Scholarshipstudents) to state-of-the-art research skills and in-depth knowledge of advanced biology,
chemistry and physics to harness the potential of biotechnology.
4. Having been administrating and implementing the “UNESCO Biotechnology School inAsia” (2012-) at Department of Biotechnology, Graduate School of Engineering, Osaka
University, taking over the long-termed “International Post-Graduate University Course in
Microbiology” (1972-2003) and Postgraduate Inter-University Course in Biotechnology(2004-2007) with total of 459 alumni spreading all over Asia.
5. Undertaking a JSPS Asian CORE Program entitled as ‘Next-Generation Bioproduction
Platform Leveraging Subtropical Microbial Bioresources’. This project aims to develop
next-generation bioproduction platform by interaction between excellent researchers fromJapan, Thailand, Vietnam, Laos and Cambodia, together with fostering young researchers
from those countries for future in the field of industrial biotechnology. The program is basedon the result from preceding MEXT/JST project carried out in FY2006-2008, which took
over the termination in 2004 of the JSPS multilateral program in the field of biotechnology.
6. Involving as a research group at Japan side for a JICA-JST Project in Thailand: ThailandJapan Collaboration for the Research and Development of Therapeutic Products against
Infectious Diseases. The Research Institute for Microbial Diseases (RIMD), Osaka
University, acts as the Japanese host coordinator for the project. The Project is implemented
under the “Science and Technology Research Partnership for Sustainable Development(SATREPS)” promoted by Japan International Cooperation Agency (JICA) and JST incollaboration.
7. Serving as the secretariat for the UNESCO Regional Network for Microbiology andBiotechnology in Southeast Asia in accordance with a proposed memorandum resulted froma Consortium Meeting of the Project “Tropical Bio-Resources and Green Chemistry
Strategy” (Osaka 2003).
8. Working as a member of the global network “Microbiological Resource Centers”(MIRCENs) operated by UNEP, UNESCO and ICRO; and as an original member of the“Regional Network for Microbiology in Southeast Asia” operated under the auspices of
UNESCO through the Fund-In-Trust of the Japanese Government (J-FIT).

477

9. Promoting international cooperative researches in biotechnology with the Southeast Asian
countries related to Biotechnology, under the support of Monbu-kagaku-sho Grant-in-Aids
for International Scientific Research. In addition, ICBiotech cooperates in developing
international organization and conducting academic seminars related to biotechnology.
10. Publishing Annual Reports of ICBiotech periodically.
Steering Committee
ChairmanProf. Takuya Nihira (Director of ICBiotech)Committee Members
Prof. Kazuhito Fujiyama (ICBiotech)
Prof. Satoshi Harashima (Graduate School of Engineering)
Prof. Shigeru Kanaya (Graduate School of Engineering)
Prof. Eiichiro Fukusaki (Graduate School of Engineering)
Prof. Tsuyoshi Inoue (Graduate School of Engineering)
Prof. Kazumasa Hirata (Graduate School of Pharmaceutical Sciences)Prof. Masahito Taya (Graduate School of Engineering Science)Prof. Toshihiro Horii (Research Institute for Microbial Diseases)
Staff
Director/Professor
Dr. Takuya Nihira
Professor
Dr. Kazuhito Fujiyama
Adjunct Professor
Dr. Michihiko Ike (Department of Environmental Engineering,Osaka University)

Collaborative Professor
Dr. Amaret Bhumiratana (Mahidol University, Thailand)
Dr. Watanalai Panbangred (Mahidol University, Thailand)
Dr. Skorn Mongkolsuk (Mahidol University, Thailand)
Visiting Professor
Dr. ToshiomiYoshida (Osaka University)
Dr. Suteaki Shioya (Sojo University)
Visiting Associate Professor
Dr. Michiho Ito (Kyoto University)
Visiting Research Scholar
Dr. Sutipa Tanapongpipat (BIOTEC, Thailand)
Dr. Nguyen Kim Nu Thao (Vietnam National University, Vietnam)
Associate Professor
Dr. Shigeru Kitani
LecturerDr. Ryo Misaki
Assistant Professor
Dr. Hiroshi Kinoshita
Dr. Takao Ohashi
Administrative Official Ms. Megumi Okamoto
Administrative Assistant Ms. Kiyoko Tanaka
Ms. Yukiko Seto
Technical Assistant
Ms. Chunxiang Wang478

I . COOPERATIVE RESEARCH STATION (CRS) IN SOUTHEAST ASIA
Mahidol University (MU) and Osaka University (OU) together established the Mahidol
University-Osaka University Collaborative Research Center (MU-OU:CRC) for Bioscienceand Biotechnology at Faculty of Science, Mahidol University in 2002, to strengthen the
research cooperation in these fields which are amongst the most active fields of study andresearch in both universities.
Currently, MU-OU:CRC has co-ordinated a research projects under the jointly support ofNational Research Council of Thailand (NRCT), National Center for Genetic Engineering andBiotechnology (BIOTEC) and The Japan Society for the Promotion of Science (JSPS).
Researchers from Mahidol University, Chulalongkorn University, Kasetsart University, King
Mongkut's University of Technology Thonburi and BIOTEC participate in this project.
CRS is conducting researches on
1. Strain improvement of Bacillus thuringiensis.
2. Screening of actinomycetes for bioactive compound.
3. The soil metagenome for screening of potential polysaccharide-degrading enzymes.
4. Cell culture system for screening of bioactive compounds from Streptomycetes andenvironmental fungi.
5. Quorum sensing interference: Novel biocontrol strategies for pathogenic bacteria.
II. UNESCO COURSES”UNESCO International Post-Graduate University Course in Microbiology” (1973-2003)
plus the “UNESCO Postgraduate Inter-University Course in Biotechnology” (2004-2007) was
one of the two long-term training courses held in Japan launched in response to therecommendations concluded at a UNESCO General Conference Meeting. After the operationof 33 batches for developing human resources in Asia, total 459 young researchers from allover Asian countries are now playing active and leading roles in current biotechnology researchand education in the region to contribute to the development of their own country, andconsequently to Asian countries, and the establishment of the closer and stronger network in
the region.
UNESCO Training Project – One-month Lab Revisit ProgramThis project was conducted in August-September 2012, under the Official Development
Assistance Grants for UNESCO Activities of MEXT, based on the result of the Conference
Series. Participants were alumni of the previous UNESCO courses on Microbiology andBiotechnology, or those nominated by the senior alumni in their home laboratories.
Purpose
1. To provide the opportunity for the alumni and related young researchers to visit
laboratories in Japanese universities to be exposed to leading-edge of research, toupgrade research skills, and to facilitate exchange of researchers.
2. To rebuild up the networks among researchers between Japan and Asian countries in
the field of biotechnology.
3. To strengthen the network among the alumni of our UNESCO courses beyond
generations and countries.

479

List of ParticipantsGroup A
Name

Country

Affiliation

Host University

Myra Tansengco

Philippine

Industrial Technol.
Development Inst.

Prof. Atomi,
Kyoto U

Rakhi Chaturvedi

India

Indian Inst. ofTechnol.

Prof. Fujiyama,
Osaka U

Rujirek Noisangiam

Thailand

Suranaree U of
Technology

Prof. Minamisawa,
Tohoku U

Past UNESCO Course
UM2711 (1999-00)
Nominated by Dr. V. S.
Bisaria (UM0103, IIT-Delhi)
Nominated by Dr. N.
Tiaamroeng (UM1712,
Suranaree U of Technol.)Song Liu

China

Sch. Biotechnol.,
Jiangnan U

Prof. Kanaya,
Osaka U

Nominated by Prof. Chen

PannidaKhunnamwong

ThailandDept. Microbiol.,
Kasetsart U

Prof. Fujiyama,
Osaka U

Nominated by Dr. Savitree

Nattika Saengkrit

Thailand

NANOTEC, National

Prof. Nihira,
Osaka U

UM2907(2001-02)

Azeem Khalid

Pakistan

Prof. Nihira,
Osaka U

UM3007(2002-03)

Sci. and Technol.
PMAS Arid Agric. U
Rawalpindi

Jian (UM2101, Jiangnan U)

Limtong (Kasetsart U)

Group B
Name

Country

Lien Ha Tran

VietnamAnak Khantachawana

ThailandAffiliation
Host University
Hanoi U of Sci. and Prof. Sonomoto,Technol.
Kyushu U
Biol. Eng. Program, Prof. Kinooka,
KMUTTOsaka Uy

Past UNESCO Course
UM2413(1996-97)
Nominated by
Osaka U

UM: International Post-Graduate University Course in Microbiology
UB: International Inter-University Course in Biotechnology

Schedule
Group A:
August 29
August 30
August 31
September 1
September 2
September 27
September 28
October 31
Group B:
August 29
August 30
August 31
September 1

Arrival in Osaka
Orientation and lab tour at Osaka U.
Lectures and Discussion meeting on the new UNESCO program
Move to assigned universities
Research and Discussion at each university
Gather at Osaka U., Submission of report
Departure from Osaka
Submission of final report
Arrival in Osaka
Orientation and lab tour at Osaka U.
Lectures and Discussion meeting on the new UNESCO program
Move to assigned universities

480

September 2
September 6
October 31

Research and Discussion at each university
Gather at Osaka U., Submission of report
Departure from Osaka
Submission of final report

Program of Lectures and Discussion
30 August 2012
Venue: Suntory Memorial Hall (5th floor of Department of Biotechnology (C3))
13:30-13:40 Opening remarks by Prof. Takuya Nihira (Director of ICBiotech, Osaka U)
13:40-13:50 Opening remarks by Prof. Hubert J. Gijzen, PhD (Director UNESCO Regional Science Bureau forAsia and the Pacific)
13:50-14:00 Opening remarks by Mr. Takashi Asai (Deputy Secretary-General, Japanese National Commission
for UNESCO, MEXT)
14:00-14:10 Opening remarks by Prof. Toshiya Muranaka (Advanced Sci. and Biotechnol., Grad. Sch. Eng.,
Osaka U)
14:10-14:30 Break
14:30-17:00 Self introduction from each participant of the program
Venue: 'La Scena', 15th Floor of GSE Common Building
17:30Reception

31 August 2012
Venue: Suntory Memorial Hall (5th floor of Department of Biotechnology (C3))
10:30-11:45 “Biotechnology – the next science-based revolution?” Prof. Hubert J. Gijzen, PhD (Director
UNESCO Regional Science Bureau for Asia and the Pacific)
11:45-13:30 Lunch Break
Venue: Meeting room C3-432 (4th floor of Department of Biotechnology (C3))
13:30-14:15 “Structural and functional studies of enzymes from extremophiles and their application”
Dr. Yuichi Koga (Laboratory of Molecular Biotechnology, Osaka U)
14:15-15:00 “Actinomycetes, wonderful microorganisms for production of useful natural compounds”
Dr. Shigeru Kitani (Associate Prof., ICBiotech, Osaka U)
15:00-15:10 Break
15:10-15:55 “Yeast genetic resources and yeast PHO pathway”
Dr. Yoshinobu Kaneko (Yeast Genetic Resources Lab, Endowed Chair, Osaka U)
16:00-17:30 Introduction of the Current Department of Biotechnology
17:30Discussion on the previous/new UNESCO courses

UNESCO Biotechnology School in Asia (JFIT Project)
The “UNESCO Biotechnology School in Asia” was launched in October 2012. This
program is an activity of the “UNESCO Extra-budgetary Program for Human Resources andResearch Network in Sciences and Technology” supported by the Japanese Fund-In-Trust(JFIT). The course is a program for the scientific training for young Asian university- graduatesin the field of Biotechnology and related areas resulting in achieving master degree, and theparticipants are expected to make significant contributions to an educational or scientific481

institution in the coming years in their home countries. It aims also to strengthen the network
activities among the biotechnology scientists in the region.
[1st cohort students]
Home
country

NameCountry to beallocated

University to Study

Cambodia

Ung Sivlin

ThailandKMUTT

Dr. Kanokwan Poomputsa

Cambodia

Chhon Yanvary

Thailand

KMUTT

Dr. Paitip Thiavetyan

CambodiaBan MabThailand

Mahidol U

Dr. Manop Supantharika

Cambodia

Sieng Darith

Thailand

Mahidol U

Dr. Chuenchit Boonchird

Laos

Phonepaseuth
Phongphayboun

Thailand

Chulalongkorn U

Dr. Ekawan Luepromchai

Laos

Soulyavong
Souphaline Liko

Indonesia

Institut Teknologi
Bandung

Dr. I Nyoman P. Aryantha

VietnamTruong Ngoc Quang

ThailandKasetsart U

Dr. Savitree Limtong

Thailand

Chalisa Jaturapaktrarak

Japan

Osaka UDr. Hisao Ohtake

Indonesia

Fitria Ayuningtyas

Japan

Osaka U

Dr. Masahiro Kino-oka

Philippines

Mark Anthony Alfredo
Badua

Japan

Osaka UDr. Satoshi Harashima

Indonesia

Dwi Purno Widekdo

Japan

Osaka U

Dr. Kazuhito Fujiyama

Assigned Supervisor

III. JSPS ASIAN CORE PROGRAM: NEXT-GENERATION BIOPRODUCTION
PLATFORM LEVERAGING SUBTROPICAL MICROBIAL BIORESOURCES(FY2009-2013)
Following the termination of the JSPS multilateral program in the field of Biotechnology
under the framework of Core-University System conducted for the past 27 years since 1978, ascheme of collaboration of Japan Science and Technology Agency (JST) was carried out in
FY2006-2008 as a program of "Solution of Research Issues Shares by Asian Region” under theframework of “Asian Science and Technology Cooperation Promotion Strategy" supported by
the Special Coordination Funds for Promotion of Science and Technology (SCF) of MEXT.
This project aims to develop next-generation bioproduction platform by interaction between
excellent researchers from Japan, Thailand, Vietnam, Laos and Cambodia, together with
fostering young researchers from those countries for future in the field of industrial
biotechnology.

482

1. Purpose
Based on the result from preceding MEXT/JST project carried out in FY2006-2008, theproject area is expanded widely to Thai neighbouring countries (Vietnam, Laos and Cambodia)
in addition to Thailand, and research exchange will be carried out focusing on development of
bioproduction platform using microbes.
Thailand, Vietnam, Laos and Cambodia belong to countries having subtropical climate,
and thus have abundant bioresources, such as agro/marine resources. Especially, microbial
resources as invisible bioresources are highly abundant, possessing diverse array of unexploredmicroorganisms which are not present in Japan. These novel useful bioactive compounds (suchas antibiotics and bacteriocins), producers of useful industrial enzymes (phytase of soft biomass
degrading enzymes), whole-cell catalyst to produce bioethanol or sugars through degradationof soft biomass or the biocatalyst replacing present toxic inorganic catalyst in industrial
processes in converting hydrophobic compounds. On-site bioproduction platform using
Southeast Asian bioresources is essential as the next-generation fundamental technology. This
project aims to develop next-generation bioproduction platform by interaction between
excellent researchers from Japan, Thailand, Vietnam, Laos and Cambodia, fostering young
researchers from those countries for future in the field of industrial biotechnology.
2. Outline of Implementation System
Japanese academic consortium will be formed from professional academic staffs belonging
to top five Japanese universities (Osaka University, The University of Tokyo, Hokkaido
University, Kyushu University and Toyama Prefectural University) who are excellent in several
steps of industrial biotechnology. Similarly, Thai academic consortium will be formed from
representative young researchers in the field of biotechnology from Thai top 4 universities(Mahidol University, Chulalongkorn University, Kasetsart University, and King Mongkut’s
University of Technology Thonburi) and BIOTEC (National Center for Genetic Engineeringand Biotechnology). The international collaboration will be performed between the Japaneseconsortium and the Thai consortium to design and establish efficient way in the utilization ofbioresources.
Japanese core organization: Osaka University
Japanese coordinator: Prof. Takuya Nihira, ICBiotech, Osaka University
Counterpart country and core organization:
Prof. Watanalai Panbangred, Mahidol University, Thailand
Dr. Duong Van Hop, The Institute of Microbiology and Biotechnology, Vietnam
National University, Hanoi, Vietnam
Mr. Sokunthia Thao, Royal University of Phnom Penh, Cambodia
Dr. Sundara Souriuodong, Science and Technology Research Institute, National
Authority for Science and Technology, Laos
3. Research Topics and Participating Members (Group leader*)Group 1. Production of novel bioactive compounds
Japan: Takuya Nihira*(ICBiotech, Osaka U), Eiichiro Fukusaki (Osaka U), Kazumasa Hirata(Osaka U), Yasuhiro Igarashi (Toyama Prefectural U), Kenji Sonomoto (Kyushu U)
Thailand: Watanalai Panbangred (Mahidol U), Amaret Bhumiratana (Mahidol U),
Patoomratana Tuchinda (Mahidol U), Amonlaya Tosukhowong (BIOTEC),
Wonnop Visessanguan (BIOTEC), Ruud Valyasevi (BIOTEC)

483

Vietnam: Duong Van Hop (IMBT, Vietnam National U Hanoi)
Cambodia: Hang Chan Thon (Royal U of Phnom Penh), Thao Sokunthia (Royal U of Phnom
Penh)
Laos:
Sourioudong Sundara (Laos National Authority of Science and Technology)
Group 2. Production of novel useful enzymes
Japan: Kazuo Sakka* (Mie U), Kozo Asano (Hokkaido U)
Thailand: Vithaya Meevootisom (Mahidol U), Suthep Wiyakrutta (Mahidol U),
Khanok Ratanakhanokchai (King Mongkut's U of Technology Thonburi)
Vietnam: Duong Van Hop (IMBT, Vietnam National U Hanoi)Group 3. Development of novel biocatalysts
Japan: Satoshi Harashima* (Osaka U), Hisao Ohtake (Osaka U), Kazuhito Fujiyama (Osaka U),
Yasuo Igarashi (U of Tokyo)
Thailand: Savitree Limtong (Kasetsart U), Wichien Yongmanitchai (Kasetsart U),
Chuenchit Boonchird (Mahidol U), Thipa Asvarak (Mahidol U),
Thunyarat Pongtharangkul (Mahidol U), Alisa Vangnai (Chulalongkorn U),
Verawat Champreda (BIOTEC)

4. Seminar and Symposium
The Young Scientist Seminar 2012
In the framework of the JSPS Asian Core Program (2009 – 2013)
Bioresources: Potential & Applications
Date: October 4 -5th, 2012
Venue: Institute of Microbioloy and Biotechnology, Conference Room 601
E2 Building, 144 Xuan Thuy, Ha Noi, Vietnam
www.imbt.vnu.edu.vn

Hosted by: Institute of Microbiology and Biotechnology (IMBT)
Vietnam National University Hanoi (VNUH)In Association with: Osaka Univ. (OU)
Japan Society for the Promotion of Science (JSPS)
Organizing Committee
Assoc. Prof. Dr. Duong Van Hop (IMBT, VNUH, Vietnam)
Prof. Dr. Takuya Nihira (ICBiotech, Osaka U, Japan)
Secretary
Vietnamese Side
Dr. Dinh Thuy Hang, Dr. Trinh Thanh Trung, Dr. Nguyen Kim Nu Thao (IMBT,VNUH)Japanese Side
Dr. Takuya Nihira, Dr. Hiroshi Kinoshita, Ms. Yukiko Seto (ICBiotech, Osaka U)

484

Program Schedule
Oral Presentations<Day 1: October 4th, 2012>
8:00 – 8:30 Registration
8:30 – 8:40 Opening Ceremony
Welcome Address by Assoc. Prof. Dr. Duong Van Hop, Director of IMBT
Prof. Dr. Takuya Nihira, Osaka U, Project Leader
8:40 – 9:20 Invited speaker (I-1) Prof. Dr. Hisao Ohtake, Osaka U, JapanThe sustainable use of phosphorus - A new global challenge
9:20 – 10:00 Keynote Lecture 1 (K-1) Prof. Dr. Kozo Asano, Hokkaido U, Japan
Indigestible carbohydrates and intestinal microbiota
Section A: Microbial physiology (Chairperson: Mr. Yasin Kitichantaropas/Mr. Keiichi Ishido)
10:00 – 10:20 Oral Presentation 1 (O-1) Assoc. Prof. Dr. Minetaka Sugiyama, Osaka U, Japan
Mechanisms of lactic acid tolerance in Saccharomyces cerevisiae
10:20 –10:40 (O-2): Mr. Yasin Kitichantaropas, Mahidol U, Thailand
Cellular mechanisms for tolerance to environmental stresses in thermotolerant Saccharomyces
cerevisiae
10:40 – 11:00 Coffee break
11:00 – 11:20 (O-3) Ms. Sharmin Dilruba, Osaka U, Japan
Functional interrelationship of PP2C protein phosphatases as revealed by phenotypic analysis of aseries of multiple disruptants in Saccharomyces cerevisiae
11:20 – 11:40 (O-4) Mr. Takenori Kanazawa, Osaka U, Japan
Characterization of URA3 gene in oleaginous yeast Rhodosporidium toruloides DMKU3-TK16
for lipid engineering
11:40 – 12:00 (O-5) Mr. Keiichi Ishido, Osaka U, Japan
Analysis of secondary metabolites in entomopathogenic fungi
12:00 – 14:00 Lunch & Poster Presentations
Section B: Whole cell catalyst and enzymes (Chairperson: Mr. Takashi Imagawa/Ms. Le Thi HoangYen)
14:00 – 14:20 (O-6) Assoc. Prof. Dr. Kohsuke Honda, Osaka U, Japan
Synthetic metabolic engineering - A novel and simple technology for freely designing an artificial
metabolic pathway
14:20 – 14:40 (O-7) Ms. Nhuengtida Chanarpakorn, Mahidol U, Thailand
Enhanced regio-selective alkane hydroxylation via coupled enzyme system
14:40 – 15:00 (O-8) Mr. Takashi Imagawa, Osaka U, Japan
Construction of the artificial pyruvate oxidation pathway by synthetic metabolic engineering
15:00 – 15:20 (O-9) Assist. Prof. Dr. Takao Ohashi, Osaka U, Japan
Glycoengineering in the fission yeast Schizosaccharomyces pombe - a promising technology forthe production of glycoproteins
15:20 – 15:40 Coffee break
15:40 – 16:00 (O-10) Ms. Pirapan Polburee, Kasetsart U, Thailand
Selection and taxonomic characterization of oleaginous yeast for lipid production from glycerol
16:00 – 16:20 (O-11) Ms. Le Thi Hoang Yen, IMBT - VNU Hanoi, Vietnam
Study on xylanase from Aspergillus niger VN09-0119 isolated from a preserved forest in Vietnam
16:20 – 16:40 (O-12) Mr. Paripok Phitsuwan, King Mongkut's TU Thonburi, Thailand
Tepidimicrobium xylanilyticum BT14 produces multienzyme complexes to degrade plant cell wall
16:40 – 17:00 (O-13) Ms. Tran Thi Loan, IMBT - VNU Hanoi, Vietnam
Effects of some plant proteinase inhibitors from Momordica coinchinensison on several meatspoilage bacteria
18:00 Welcome dinner (Japanese-Vietnamese Restaurant Nagomi Hanoi)<Day 2: October 5th, 2012>
8:30 – 9:10 (K-2) Prof. Dr. Yasuhiro Igarashi, Toyama Pref. U, Japan
Microbial secondary metabolites: potential source of structural diversity for drug discovery.
Section C: Novel compounds (Chairperson: Mr. Linkai Yu/Ms. Notthaporn Phrueksahiran)485

9:10 – 9:30 (O-14) Assist. Prof. Dr. Naoya Oku, Toyama Pref. U, Japan
Complete stereochemistry of rakicidin A, an actinomycetal cytotoxic depsipeptide
9:30 – 9:50 (O-15) Ms. Notthaporn Phrueksahiran, Mahidol U, Thailand
Screening and identification of antifungal metabolites from Streptomyces spp
9:50 – 10:10 (O-16) Ms. Nghiem Phuong Hien, IMBT-VNU Hanoi, Vietnam
Primary study on protease inhibitors from bacteria and actinomycete
10:10 – 10:30 (O-17) Mr. Linkai Yu, Toyama Pref. U, JapanThe structures and activities of new anti-invasive compounds from Streptomyces sp.
10:30 – 10:50 Coffee break
Section D: Biosynthesis (Chairperson: Ms. Fuqin Mu/Ms. Chinatsu Shimafuji)
10:50 – 11:10 (O-18) Assist. Prof. Dr. Hiroshi Kinoshita, Osaka U, Japan
Development of the genetic techniques to utilize biosynthetic genes for secondary metabolites
from filamentous fungi
11:10 – 11:30 (O-19) Assist. Prof. Dr. Takeshi Zendo, Kyushu U, Japan
Screening and characterization of novel bacteriocins from lactic acid bacteria
11:30 – 11:50 (O-20) Ms. Chinatsu Shimafuji, Kyushu U, Japan
In vitro catalytic activity of N-terminal and C-terminal domains in NukM, the modification
enzyme of lantibiotic nukacin ISK-1
11:50 – 12:10 (O-21) Ms. Fuqin Mu, Kyushu U, Japan
Analysis of gene cluster involved in production of the circular bacteriocin leucocyclicin Q by
Leuconostoc mesenteroides TK41401
12:10 – 14:00 Lunch
Section E: Whole cell metabolites and metagenome(Chairperson: Mr. Shinya Yamamoto/ Dr. Phan Thi Phuong Hoa)
14:00－14:20 (O-22) Mr. Shinya Yamamoto, Osaka U, Japan
Metabolite profiling of soy sauce using gas chromatography with Time-of-Flight Mass
spectrometry and analysis of correlation with quantitative descriptive analysis
14:20 – 14:40 (O-23) Mr. Hirofumi Akiyama, Toyama Pref. U, Japan
Temperature-dependent metabolic change and exhaustive metabolite analysis in Thai
endophytic Microbispora
14:40 – 15:00 (O-24) Mr. Kazuki Yamamoto, Toyama Pref. U, Japan
Analysis of secondary metabolism in a thermophilic Gram-positive bacterium Thermosporothrix
belonging to the phylum Chloroflexus
15:00 – 15:20 (O-25) Dr. Phan Thi Phuong Hoa, School of Medicine and Pharmacy, VNUH
Screening for actinomycetes isolated from soil with the ability to inhibit Xanthomonas oryzae pv.
oryzae causing rice bacterial blight disease in Vietnam
15:20 – 15:40 Coffee break
15:40 – 16:00 (O-26) Dr. Wuttichai Mhuantong, NIBB, Thailand
Comparative analysis on metabolic capabilities of metagenomes from lignocellulose degrading
environments
16:00 – 16:20 (O-27) Mr. Keita Kato, Kyushu U, Japan
Microecological charaterization of dominant Lactobacillus species in nukadoko, a fermented
rice bran used for Japanese pickles
16:20 – 16:40 (O-28) Ms. Nguyen Thi Hoa, IMBT-VNU Hanoi, Vietnam
Degradation characteristics of microcystin by isolated bacteria from Hoan Kiem Lake, Hanoi
16:40 – 17:20 Closing Ceremony
Presentation Award Ceremony
Closing Remark by Prof. Dr. Takuya Nihira

Poster Presentations
P-1 (Section D) Mr. Sor Ratha (Royal U of Phnom Penh, Cambodia)
Potential of rotifers as bioindicator for freshwater quality: A case study in the upper part ofCambodian Mekong river basin
P-2 (Section C) Mr. Korm Sovannarith (Royal U of Phnom Penh, Cambodia)
Screening of Streptomyces active against plant pathogens
P-3 (Section C) Mr. Keo Phommavong (Biotechnology and Ecology Institute, Laos)

486

Endophytic actinomycetes with inhibitory activity against bacterial pathogens
P-4 (Section C) Ms. Bui Thai Hang (College of Food Industry, Danang, Vietnam)
Screening LAB for bacteriocin production from fermented foods in Vietnam
P-5 (Section A) Ms. Nguyen Thu Hoai (Vietnam-Russia Tropical Center, Hanoi)
Methane fermentation of seaweed in saline water condition
P-6 (Section C) Mr. Dao Xuan Duong (Hanoi Open U)
Primary screening bacteriocin producing strains from Vietnamese vegetables
P-7 (Section C) Ms. Tran Thi Trang (IMBT, Hanoi, Vietnam)
Diversity of subtilisin genes of bacteria isolated from some traditional fermented foods in Vietnam
P-8 (Section B) Ms. Phan Thi Mai (U of Sciences, VNUH)
Production and characterization of thermostable phytase from Bacillus amyloliquefaciens SP19.1
isolated from Hoang Lien National Park soil
P-9 (Section B) Ms. Tran Thi Le Quyen (IMBT, Hanoi, Vietnam)
Optimization of thermostable xylanase production by Streptomyces misionensis in solid-state
fermentation
P-10 (Section A) Ms. Luu Thi Phuong (U of Eng. and Technol., VNUH)
Study on characteristic green algae Botryococcus strains isolated in Vietnam

List of Participants
VIETNAM
Assoc. Prof. Dr. Duong Van Hop
Dr. Nguyen Huynh Minh Quyen
Ms. Phan Thi Ha
Dr. Dinh Thuy Hang
Dr. Nguyen Quynh Uyen
Dr. Nguyen Thi Hoai Ha
Dr. Nguyen Kim Nu Thao
Dr. Dao Thi Luong
Dr. Trinh Thanh Trung
Ms. Le Thi Hoang Yen
Mr. Nguyen Anh Tuan
Ms. Tran Thi Le Quyen
Ms. Nghiem Phuong Hien
Ms. Tran Thi Loan
Ms. Tran Thi Trang
Ms. Hoang Thu Ha
Dr. Phan Thi Phuong Hoa
Mr. Dao Xuan Duong
Ms. Nguyen Thu Hoai
Ms. Bui Thai Hang
Ms. Phan Thi Mai
Ms. Nguyen Thi Hoa
Ms. Luu Thi Phuong
CAMBODIA
Dr. Sokunthia Thao
Mr. Sor Ratha
Mr. Korm Sovannarith
LAOS
Dr. Kosonh Xayphakatsa
Mr. Keo Phommavong
THAILAND
Prof. Dr. Watanalai Panbangred
Dr. Verawat Champreda
Dr. Wuttichai Mhauntong

Director of Institute of Microbiology and Biotechnology (IMBT)
Vietnam National University Hanoi (VNUH)
Deputy Director IMBTHead of the Administration Office, IMBTHead of Microbial Ecology Lab., IMBTHead of General Lab., IMBTHead of Algal Biology Lab., IMBTHead of Microbial Biotechnology R&D Lab., IMBT
Vice Director, Vietnam Type Culture Collection (VTCC) , IMBTVTCC, IMBTVTCC, IMBTVTCC, IMBTVTCC, IMBT
Protein and Enzyme Lab., IMBT
Protein and Enzyme Lab., IMBT
Protein and Enzyme Lab., IMBT
Protein and Enzyme Lab., IMBT
School of Medicine and Pharmacy, VNUH
Hanoi Open University
Vietnam-Russia Tropical CenterCollege of Food Industry, Danang
Hanoi University of Science, VNUH
University of Engineering and Technology, VNUH
Algal Biology Lab., IMBT, VNUHFaculty of Science, Royal University of Phnom Penh
Faculty of Science, Royal University of Phnom Penh
Faculty of Science, Royal University of Phnom Penh
BEI, Ministry of Science and Technology
BEI, Ministry of Science and Technology
Faculty of Science, Mahidol University
National Center for Genetic Engineering and Biotechnology
National Center for Genetic Engineering and Biotechnology

487

Ms. Nhuengtida Chanarpakorn
Ms. Notthaporn Phrueksahiran
Mr. Yasin Kitichantaropas
Ms. Pirapan Polburee
Mr. Paripok Phitsuwan
JAPAN
Prof. Dr. Takuya NihiraProf. Dr. Hisao Ohtake
Prof. Dr. Kozo Asano
Prof. Dr. Yasuhiro Igarashi
Ass. Prof. Dr. Kohsuke Honda
Dr. Hiroshi Kinoshita
Ass. Prof. Dr. Minetaka Sugiyama
Dr. Takao Ohashi
Ms. Sharmin Dilruba
Mr. Shinya Yamamoto
Mr. Takashi Imagawa
Mr. Keiichi Ishido
Mr. Takenori Kanazawa
Dr. Takeshi Zendo
Ms. Chinatsu Shimafuji
Ms. Fuqin Mu
Mr. Keita Kato
Dr. Naoya Oku
Mr. Kazuki Yamamoto
Mr. Hirofumi Akiyama
Mr. Linkai Yu
Mr. Kenichi Wada
Ms. Sayaka Minami
Ms. Yukiko Seto

Faculty of Science, Mahidol University
Faculty of Science, Mahidol University
Faculty of Science, Mahidol University
Faculty of Science, Kasetsart University
King Mongkut's University of Techonology ThonburiInternational Center for Biotechnology, Osaka University
Graduate School of Engineering, Osaka University
Graduate School of Agriculture, Hokkaido University
Biotechnology Research Center, Toyama Prefectural University
Graduate School of Engineering, Osaka University
International Center for Biotechnology, Osaka University
Graduate School of Engineering, Osaka University
International Center for Biotechnology, Osaka University
Graduate School of Engineering, Osaka University
Graduate School of Engineering, Osaka University
Graduate School of Engineering, Osaka University
Graduate School of Engineering, Osaka University
Graduate School of Engineering, Osaka University
Faculty of Agriculture, Kyushu University
Faculty of Agriculture, Kyushu University
Faculty of Agriculture, Kyushu University
Faculty of Agriculture, Kyushu University
Biotechnology Research Center, Toyama Prefectural University
Biotechnology Research Center, Toyama Prefectural University
Biotechnology Research Center, Toyama Prefectural University
Biotechnology Research Center, Toyama Prefectural University
Department of International Affairs Administration Bureau, Osaka University
Department of International Affairs Administration Bureau, Osaka University
International Center for Biotechnology, Osaka University

5. Committee Meeting
July 2, 2012. Domestic Members Meeting at ICBiotech, Osaka University, attended by
Japanese cooperative researchers.
January 21, 2013. Domestic Members Meeting at ICBiotech, Osaka University, attended by
Japanese cooperative researchers.

IV. JSPS BILATERAL PROGRAM OF JOINT RESEARCH PROJECT AND
SEMINARS –VIENAM (August 2011-March 2014)
JSPS promotes international scientific exchanges between Japan and counterpart countries
in accordance with agreements or memoranda of understanding concluded with academies,
research councils and other science-promotion organizations in those countries. ICBiotech
participates in the bilateral programs agreed between Vietnam Academy of Science andTechnology (VAST), which mainly take the form of joint research projects, joint seminars andresearcher exchanges.

488

V. JASSO SHORT STAY & SHORT VISIT (SS & SV) PROGRAM(April 2012-March 2013)
Short-term stay and short-term visit program (SS&SV) by the Japan Student Services
Organization (JASSO) is a program of both student acceptance and dispatch by Japanese
universities. It must be conducted under each Japanese university’s control and responsibility.
JASSO provides selected students with monthly stipend of less than 3 months. In 2012, 10
Japanese MS students stayed in universities in Thailand (Mahidol University, Chulalongkorn
University, Kasetsart University, King Mongkut’s University of Technology Thonburi), andconducted research for 4 weeks, August 5-September 9, 2012). Whereas 6 students (Mahidol
University, King Mongkut’s University of Technology Thonburi, Kasetsart University) fromThailand conducted research from July 5 to August 2, 2012, in the laboratories of Prof.
Harashima, Prof. Nihira and Prof. Fujiyama at the Graduate School of Engineering, Osaka
University.
VI. JST-JICA (SATREPS) (2008-2012)

SATREPS (Science and Technology Research Partnership for Sustainable Development) isa Japanese government program that promotes international joint research targeting global
issues. SATREPS projects are expected to lead to outcomes with potential for practicalutilization, and to enhance research capacity in the developing country. The program isconducted through collaboration between two Japanese government agencies: the Japan
Science and Technology Agency (JST) and the Japan International Cooperation Agency (JICA).
ICBiotech works as a cooperative group to the following project.
Topic: Research and Development of Therapeutic Products against Infectious Diseases,
especially Dengue Virus Infection Creating Drugs Effective against the Dengue
Virus from Human Beings.
Principal Investigator: Prof. IKUTA Kazuyoshi/Research Institute for Microbial Diseases,
Osaka University.
Research Period: 4 years from 2008.
Counterpart Research Institutions: Ministry of Public Health-National Institute of Health(NIH), Mahidol University (Thailand).
Research Project:
Preventing the further spread of dengue fever in Southeast Asia: Dengue fever is a viraldisease spread by mosquitoes that infects 50 million people living in the tropics every
year, with 250,000 suffering seriously as a result, but no effective therapies have yet
been discovered. Researches are for studying patients and microorganisms fromThailand to contribute to the development of drugs effective against dengue fever.
Since the human body creates proteins (antibodies) to combat the dengue virus,
investigating these proteins to find any that appear particularly effective against thevirus and also searching microorganisms prevalent in Thailand for compounds thatblock the virus.
Conducting experiments to develop new drugs following the successful creation of
antibodies: Having succeeded in creating many human-derived antibodies against
the dengue virus, and also setting a course of action for research on influenza virus
and botulinum toxin, the project will conduct animal and other experiments to assess489

the effectiveness of antibodies showing the most potential with the aim of developing
new therapeutic drugs.
VII. SCIENTIST EXCHANGES
From Japan to counterpart countries
Name
Affiliation

Takuya Nihira

Kazuhito Fujiyama

Director, Prof., ICBiotech,
Osaka U

Prof., ICBiotech, Osaka U

Visiting Period

Host Organization

April 9-14, 2012

Mahidol U

May 9-13, 2012

Philippine Society for
Microbiology

May 18-26, 2012

UNESCO Jakarta

June 10-16, 2012

Mahidol U, National Authorityof Sci. and Technol.

July 29- Aug. 7, 2012

Mahidol U

Sept. 2-8, 2012

Mahidol U, hnom Penh U

Oct. 1-8, 2012

YSS Hanoi, VNUH

Oct. 14-18, 2012

Mahidol U

Nov. 19-25, 2012

Mahidol U

Dec. 15-21, 2012

Mahidol U

Dec. 26-29, 2012

Mahidol U

Jan. 10-19, 2013

Mahidol U

Feb. 26- Mar. 5, 2013

Mahidol U

March 19-24, 2013

Mahidol U

April 9-12, 2012

Mahidol U

May 20-25, 2012

UNESCO Jakarta

June 7-10, 2012

Kasetsart U

Aug. 4-8, 2012

KMUTT

Oct. 4-5, 2012

YSS Hanoi, Vietnam

Oct. 13-18, 2012

Italian Natl. Agency for New
Technology

Nov. 12-15, 2012

Mahidol U

Dec. 5-9, 2012

Mahidol U

Dec. 16-20, 2012

Mahidol U

Feb. 26- Mar. 1, 2013

Mahidol U

Mar. 4-8, 2013

KMUTT

490

Satoshi Harashima

Prof., Grad. Sch. of Eng.,
Osaka U

July 30- Aug. 3, 2012

Mahidol U

Prof., Grad. Sch. ofOct. 1-7, 2012
Agriculture, Hokkaido U
Prof., Biotechnol. Res.
Oct. 2-7, 2012
Center, Toyama Pref. U
Assist. Prof., Biotechnol. Res.
Oct. 4-5, 2012
Center, Toyama Pref. U

YSS Hanoi, Vietnam

Kazuki Yamamoto

Ph.D. student, Biotechnol.
Res. Center, Toyama Pref. U

Oct. 4-5, 2012

YSS Hanoi, Vietnam

Oct. 4-5, 2012

YSS Hanoi, Vietnam

Hirofumi Akiyama

Ph.D. student, Biotechnol.
Res. Center, Toyama Pref. U

Oct. 6-Nov. 1, 2012

Mahidol U

Kozo Asano
Yasuhiro Igarashi
Naoya Oku

YSS Hanoi, Vietnam

YSS Hanoi, Vietnam

Wu Lin Ghai

Ph.D. student, Biotechnol.
Res. Center, Toyama Pref. U

Oct. 4-5, 2012

YSS Hanoi, Vietnam

Hisao Otake

Prof., Grad. Sch. Eng., OsakaU

Oct. 3-7, 2012

YSS Hanoi, Vietnam

Assoc. Prof., Grad. Sch.
Eng., Osaka U

July 29- Aug. 5, 2012

Mahidol U

Kohsuke Honda

Oct. 4-5, 2012

YSS Hanoi, Vietnam

Oct. 4-5, 2012

YSS Hanoi, Vietnam

Dec. 2-12, 2012

Phnom Penh U, Cambodia

July 16-21, 2012

Mahidol U

Lecturer, ICBiotech, Osaka U Dec. 16-20, 2012

Mahidol U

Mar. 12-16, 2013

Mahidol U

July 19- Aug. 2, 2012

VNUH, Vietnam

Sept. 24-, 2012

VNUH, Vietnam

Oct. 2-3, 2012

Hanoi U of Sci. and Technol.,
MOST, JICA, Vietnam

Oct. 4-5, 2012

YSS Hanoi, Vietnam

Aug. 21-28, 2012

Kasetsart U, Thailand

Oct. 3-7, 2012

YSS Hanoi, Vietnam

Oct. 3-7, 2012

YSS Hanoi, Vietnam

Jan. 27- Feb. 3, 2013

Mahidol U

Shigeru Kitani

Ryo Misaki

Hiroshi Kinoshita

Takao Ohashi

Assoc. Prof. ICBiotech,
Osaka U

Assist. Prof., ICBiotech,
Osaka U

Assist. Prof., ICBiotech,
Osaka U

Minetaka Sugiyama

Assoc. Prof., Grad. Sch. Eng.,
Osaka U

Dilruba SharminStudent, Grad. Sch. Eng.,
Osaka U

Oct. 4-5, 2012

YSS Hanoi, Vietnam

Shinya Yamamoto

Student, Grad. Sch. Eng.,
Osaka U

Oct. 4-5, 2012

YSS Hanoi, Vietnam491

Takashi Imagawa
Takenori Kanazawa
Keiichi Ishido
Takeshi Zendo
Chinatsu Shimafuji
Fuqin Mu
Keita Kato

Student, Grad. Sch. Eng.,
Osaka U
Student, Grad. Sch. Eng.,
Osaka U
Student, Grad. Sch. Eng.,
Osaka U
Assist. Prof., Fac. Agric.,
Kyushu U
Student, Fac. Agric., Kyushu
U
Student, Fac. Agric., Kyushu
U
Student, Fac. Agric., Kyushu
U

Oct. 4-5, 2012

YSS Hanoi, Vietnam

Oct. 4-5, 2012

YSS Hanoi, Vietnam

Oct. 4-5, 2012

YSS Hanoi, Vietnam

Oct. 4-7, 2012

YSS Hanoi, Vietnam

Oct. 4-5, 2012

YSS Hanoi, Vietnam

Oct. 4-5, 2012

YSS Hanoi, Vietnam

Oct. 4-5, 2012

YSS Hanoi, Vietnam

Yoshimitsu Masuda

Researcher, Fac. Agric.,
Kyushu U

Oct. 1-30, 2012

BIOTEC, Thailand

Rodney H. Perez

Student, Fac. Agric., Kyushu
U

Oct. 1-30, 2012

BIOTEC, Thailand

Hiromi Maekawa

Assoc. Prof., Grad. Sch.
Eng., Osaka U

Dec. 17-23, 2012

Mahidol U

Between counterpart countries
Name

Affiliation

Keo Phommavong

National Authority for Sci.
and Technol. (NAST) Laos

Korm Sovannarith

Royal U Phnom Penh,
Cambodia

Visiting Period

Host Organization

July 15- Sept. 15, 2012

CRS, Mahidol U, Thailand

Oct. 3-7, 2012

Vietnam National U, Hanoi

July 15- Sept. 15, 2012

CRS, Mahidol U, Thailand

Oct. 3-7, 2012

Vietnam National U, Hanoi

Sokunthia Thao

Royal U Phnom Penh

Oct. 3-7, 2012

Vietnam National U, Hanoi

Sor Ratha

Royal U Phnom Penh

Oct. 3-7, 2012

Vietnam National U, Hanoi

Xayphakatsa
KosonhMinistry of Sci. and Technol.

Oct. 3-7, 2012

Vietnam National U, Hanoi

KhanokRatanakhanokchai

KMUTT, Thailand

Oct. 7-15, 2012

Prof. Sakka, Mie U, Japan

Nguyen Kim
Nu ThaoInst. of Microbiol. andFeb. 1-, 2013
Biotechnol., VNUH, Vietnam

492

ICBiotech, Osaka U, Japan

VIII. GUESTS/VISITORS
Name

Affiliation

Staying Period

Irisa Trianti

King Mongkut’s U of Technology Thonburi

Jul. 5- Aug. 2, 2012

Vorawit Ananphongmanee

Mahidol U

Jul. 7-27, 2012

Notthaporn Phrueksahiran

Mahidol U

Jul. 11-27, 2012

Pirapan Polburee

Kasetsart U

Jul. 11-28, 2012

Rungluk Kaewwichian

Kasetsart U

Jul. 11-28, 2012

Pornkanok Chomdee

Kasetsart U

Jul. 11-28, 2012

Pumin Nutaratat

Kasetsart U

Jul. 11-28, 2012

Hubert J. Gijzen

UNESCO Jakarta

Aug. 29-31, 2012

Ousana Boonlucksanawong

Mahidol U

Sep. 2-29, 2012

Nguyen Thuy Nga

Vietnam Academy of Sci. and Technol.

Sep. 10- Oct. 9, 2012

Phan Van Kiem

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Nguyen Quang Vinh

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Trinh The Cuong

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Phan Thanh Thao

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Nguyen Quang Chinh

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Nguyen Thi Hue

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Ngo Thi Thuy Nga

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Nguyen Thi Thanh Xuan

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Dinh Van Huy

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Nguyen Xuan Binh

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Nguyen Thi Thu Thao

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Le Tat Thanh

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Le Thi Ngoc Tieu

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Tran Thi Nga

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Vu Thi Thuan

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Nguyen Ngoc Anh

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Dao Anh Khiem

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Pham Huu The

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Vuong Thi Viet Nga

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012493

Vu Thi Lan Huong

Vietnam Academy of Sci. and Technol.

Sep. 14, 2012

Marcello Donini

ENEA Biotechnology Laboratory

Sep. 18-19, 2012

Nardi Luca

ENEA Biotechnology Laboratory

Sep. 18-19, 2012

Sakarindr Bhumiratana

King Mongkut’s U of Technology Thonburi

Oct. 10, 2012

Morakot Tanticharoen

King Mongkut’s U of Technology Thonburi

Oct. 10, 2012

Anak Khantachawana

King Mongkut’s U of Technology Thonburi

Oct. 10, 2012

Apiradee Hongsthong

King Mongkut’s U of Technology Thonburi

Oct. 10, 2012

Jarunya Narangajavana

Mahidol U

Oct. 21-30, 2012

Irfan Priyambada

U Gadjah Mada

Oct. 25-26, 2012

Donny Widianto

U Gadjah Mada

Oct. 25-26, 2012

Tati Subahar

Institute Technology Bandung

Oct. 25-26, 2012

Reynaldo V. Ebora

U of the Philippines Natl. Inst. of Mol. Biol.

Oct. 25-26, 2012

Chuenchit Boonchird

Mahidol U

Oct. 25-26, 2012

Watanalai Panbangred

Mahidol U

Oct. 25-26, 2012

Savitree Limtong

Kasetsart U

Oct. 25-26, 2012

Marasri Ruengjitchatchawalya

King Mongkut's U of Technology Thonburi

Oct. 25-26, 2012

Suchada Chanprateep

Chulalongkorn U

Oct. 25-26, 2012

Duong Van Hop

VNU, Inst. of Microbiol. and Biotechnol.

Oct. 25-26, 2012

Li Phi Nga

Vietnam National U

Oct. 25-26, 2012

Francisco Banzuela Elegado

U of the Philippines Los Baños

Oct. 25-26, 2012

Ermie Bacarra

DOST

Nov. 30, 2012

Cuc Nguyen Thi

Vietnam Academy of Sci. and Technol.

Dec. 2-29, 2012

Bundit Fungtammasan

King Mongkut’s U of Technology Thonburi

Feb. 22, 2013

Chaiyong Koaykul

King Mongkut’s U of Technology Thonburi

Mar. 15-31, 2013

Nuttakrit Limjanthong

King Mongkut’s U of Technology Thonburi

Mar. 15-31, 2013

Pirawan Chantachotikul

King Mongkut’s U of Technology Thonburi

Mar. 15-31, 2013

A. K. M. Hasan Quamrul

Self employee

Mar. 13, 2013

Vu Ngoc Long

Vietnam Academy of Sci. and Technol.

Mar. 22, 2013

Le Quynh Lien

Vietnam Academy of Sci. and Technol.

Mar. 22, 2013

Pham Thi Phuong

Vietnam Academy of Sci. and Technol.

Mar. 22, 2013

Hugo Vits

Shell Technology Center Bangalore

Mar. 29, 2013494