Cell ageing and metabolism

Antioxidants
Catalase*, Gpx1, Gsr, Hsp70*,
Sod1*, Sod2*, Sod3, Prdx1,Prdx2, Prdx3, Txn1
Senescencep53, p21, p16
DNA damage
Gadd45g, Ku70, Ku80, Sirt6*
Master regulators
Sirt1†, Bach1, Nrf2
Telomere MaintenancePot1, Ku80, Trf1, Trf2
* Studies have shown increased lifespanwith over expression.
†Original paper demonstrated a 30%
increase in lifespan with over expressing
Sirt1, however out breeding reduced thisto a more modest 10%.

Discover more at abcam.com/metabolismProduced in collaboration with Sarah Barnes from the laboratory of Dr Sue Ozanne, Institute of Metabolic Science (IMS), University of Cambridge.

Copyright © 2012 Abcam, All Rights Reserved.

Cell ageing and metabolism products from Abcam

Applications/Tests

Species Reactivity

Product CodeCatalase

IHC-Fr, IHC-P, ICC, WB, IP, ELISA, ICC/IF

Ms, Rat, Shp, Chicken, Cow, Hu

ab1877

p53 Phospho S392 Human ELISA Kit

1 x 96Huab124533

Glutathione Peroxidase 1

IHC-P, WB, ICC/IF, IHC-Fr

Ms, Rat, Cow, Hu

ab22604

p53 Total + pSer46 Human In-Cell ELISA Kit (IR)1 x 96Huab128570

ab124995

p53 Total + pSer392 Human In-Cell ELISA Kit (IR)1 x 96Huab128571

Senescence Detection Kit

250

Cell culture, tissueab65351

Antioxidants | Antibodies

ProductApplications/Tests

Species Reactivity

Senescence | Kits (continued)

Glutathione Reductase [EPR7238]

WB, Flow Cyt

Ms, Rat, Hu

Hsp70 antibody [C92F3A-5]

Electron Microscopy, WB, IP, ELISA, ICC/IF,Ms, Rat, Sheep, Rab, Chicken, Guinea pig,

Flow Cyt, IHC-Fr, IHC-P

Hamster, Cow, Dog, Hu, Pig, C.elegans, Carp,ICC/IF, IHC-Fr, WB, IP, ELISA, IHC-P

Ms, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Hu

GADD45G

WB, Flow Cyt

HuPig, X.laevis, Fruit fly (Drosophila melanogaster),

Ku70 [N3H10]

IF, IHC-P, IP, WB, ICC/IF

Ms, Hu, Monkey

Fruit fly (Drosophila melanogaster), Monkey
Superoxide Dismutase 1

Product Codeab47455

DNA damage | Antibodies

Monkey, African Green Monkey

ab13498

Ku80 [5C5]WB, ELISA, IHC-P, Flow Cyt, ICC/IF

Ms, Huab3114
ab119935

SOD2 [9E2BD2]

ICC/IF, IHC-P, IP, In-Cell ELISA, Flow Cyt

Ms, Rat, Cow, Hu

ab110300

Ku70 + Ku80 [162]

ICC/IF, Flow Cyt, IHC-P, IP

Hu, X.laevis, Monkey

Superoxide Dismutase 3

IHC-Fr, IHC-P, WB

Hu

ab21974SIRT6

WB, IHC-P

Huab118487

Peroxiredoxin 1

ICC/IF, IHC-P, WB, ICC

Ms, Rat, Hu, African Green Monkey

ab15571

Anti-GAPDH + H2A.X(S139) +cleaved-PARP

Peroxiredoxin 2 antibody [EPR5154]

WB, IHC-P, ICC/IF

Ms, Rat, Hu

ab109367

antibody [3E8, 9F3 and 4B5]

WB

Huab131385

Peroxiredoxin 3 antibody [EPR8115]

WB, IP, IHC-P, ICC, Flow Cyt

Hu

ab128953

Thioredoxin

WB, ICC/IF, IHC-P

Ms, Huab26320

25

Purified damaged DNA

ab6535350

Adherent and suspension cells

ab6610860

Tissue, adherent cells

ab66110ab32441

DNA damage | Kits
DNA Damage - AP sites - Assay Kit (Colorimetric)

Antioxidants | Kits

In Situ Direct DNA Fragmentation Assay Kit

Catalase Human ELISA Kit

1 x 96

Hu, Rat, ms

ab123456(TUNEL assay)

Catalase Specific Activity Assay Kit

1 x 96

Ms, Rat, Huab118184

In Situ BrdU-Red Fragmentation Assay Kit

Glutathione Peroxidase Assay Kit

1 x 100

Cell culture supernatant, Urine, Serum, Plasma,Platelets, Other biological fluids, Tissue Extracts

Glutathione Reductase (GR) Assay Kit
Superoxide Dismutase Activity Assay Kit (Colorimetric)

ab31081 x 200
1 x 100(TUNEL assay)
ab102530

Cell culture supernatant, Urine, Serum, Plasma,

Master regulators | Antibodies

Other biological fluids, Tissue Extracts, Cell Lysate ab83461

SIRT1 [E104]

IHC-Fr, WB, ICC, IP, IHC-P

Hu

Urine, Serum, Plasma, Other biological fluids,

BACH1/BRIP1

WB

Hu

ab7288ab65354

Nrf2IHC-P, ICC/IF, IHC-Fr, WB

Ms, Rat, Hu

ab31163

ab123457

Tissue Extracts, Cell Lysate, Cell culture media
Thioredoxin (TrxR) Reductase Assay Kit

1 x 100

Cell culture supernatant, Urine, Serum, Plasma,
Other biological fluids, Tissue Extracts

ab83463

Master regulators | Kits

Total Antioxidant Capacity Assay Kit

1x100

Lysates, plasma, serum, urine

ab65329

SIRT1 Human ELISA Kit

1 x 96

Tissue Extracts, Cell Lysate

NAD/NADH Assay Kit

1x100

Lysates, plasma, serum, urine

ab65348

SIRT1 pSer47 Human ELISA Kit

1 x 96Huab135392

NADP/NADPH Assay Kit

1x100

Lysates, plasma, serum, urine

ab65349

SIRT1 Total and pSer47 Human In-Cell ELISA Kit (IR) 1 x 96

Huab135394

p53 [PAb 240]

Flow Cyt, IHC-Fr, IP, ICC/IF, WB, IHC-P

Ms, Rat, Dog, Hu

ab26

POT1WB, ICC/IF

Ms, Huab21382

p21

IHC-Fr, IP, WB, ICC/IF, ICC, Flow Cyt

Ms, Rat, Dog, Hu

ab7960

Trf1 ChIP Grade

ChIP, WB, ICC/IF, IP

Ms, Huab1423p16 ARC

ICC/IF, Flow Cyt, IP, WB, IHC-P

Ms, Rat, Dog, Hu

ab51243

Trf2

IHC-P, WB, ICC, IP, ICC/IF, Flow Cyt

Hu

ab13579

p53 Human ELISA Kit

1 x 96Huab117995

EpiSeeker ChIP Kit - One Step

48 / 96

Mammalian DNA

ab11713

p53 Phospho S46 Human ELISA Kit

1 x 96Huab124532

Senescence | Antibodies

Telomere Maintenance | Antibodies

Senescence | Kits

Telomere Maintenance | Kits

Discover more at abcam.com/metabolism

410_12_MBSProduct