005-01-09-PW Cardiac-Lineage A4:Layout 25/09/2009 07:35 Page 1

Cardiac Lineage Markers of the 2nd Heart Field
Mesodermal
precursor
BryCommon primordial
cardiovascular progenitors
Brylow / Isl-1+/- / Mesp-1
Flk-1 / c-kit?

Cardiogenic
mesoderm
Isl-1+/- / Nkx2.5low
Flk-1 / c-kit / MDR-1 / Sca-1

LegendNkx2.5 / c-kit / others

MARKER: Cell surface marker / Membrane protein

1st heart field

MARKER: Cytoplasmic protein / Secreted protein

2nd heart field

Cardiac progenitors
GATA4 / Isl-1 / MEF2c / Nkx2.5

SMC
GATA4 / GATA6SM-actin / SM-MHC / TGF β

Immature atrial
cardiomyocte
Isl-1 / sarcolipin

Atrialcardiomyocyte
cTnT / MLC2a / sarcolipin

Sinoatrial
nodal cell
Hcn4

Right ventricularcardiomyocyte
cTnT / MLC2v

Atrioventricular
nodal cell
Hcn4

Smooth muscle cell

EC:

Endothelial cell

Transient intermediates:
Differentiated progenies:

Vascular progenitors
Isl-1 / Flk-1

Immature ventricularcardiomyocte
Isl-1 / cTnT

SMC:

Bipotent progenitors:

Multipotent Isl-1+ cardiovascular progenitors
Isl-1 / Nkx2.5 / GATA4 / Flk-1

SMC precursor
Isl-1 / SM-actin

MARKER: Transcription factor / Nuclear protein

EC precursor
Isl-1 / others

Vascular SMC
precursor
Isl-1 / SM-actin

ECEts1 / GATA4
CD31 / VE-cadherin
Vimentin / vWF

Vascular SMC
GATA4 / GATA6SM-actin / SM-MHC / TGF β

The heart is composed of muscle and nonmuscle cell lineages. During cardiogenesis,
the differentiation of progenitor cells into
these multiple heart cell lineages is undertight spatial and temporal control. Within
the 2nd heart field, multipotent Isl-1+cardiovascular progenitors (MICPs) can giverise to all three major cell lineages of theheart: cardiomyocytes, smooth muscle cells(SMCs) and, to a limited extent, endothelial
cells (ECs). MICPs in the 2nd heart field giverise to cells in the right ventricle, outflow tract,and proximal coronary arteries. Less isknown about the progenitor cells of the 1stheart field and their derivatives which giverise to most of the left ventricular chamber.
Some representative cardiac lineage
markers are shown here.
1) Moretti, et al., Cell 2006
2) Wu, et al., Cell 2006
3) Kattman, et al., Dev. Cell 2006
4) Martin-Puig et al., Cell Stem Cell 20085) Murry et al., Cell 2008

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Cardiac lineage markers related antibodies from Abcam:
Product

Clonality Applications

HostSpecies
Reactivity

Datasheetwww.abcam.com/ab…

beta-catenin

P

IHC-P, WB, ICC/IF, Dot, IHC-Fr

Rb

Hu, Ms, Rat, Chk, Pig, Xl, Zfsh

6302

BMP-2

P

WB, ELISA, Dot, IHC-Fr

Rb

Hu, Ms, Rat

14933

Brachyury / Bry antibody

M

WBMs

Hu

57480

P

ICC/IF, IHC-Fr, IHC-P, WB

Rb

Hu, Ms20680

Brachyury / Bry antibody Mesoderm Marker
cardiac Troponin T antibody

P

ICC/IF, WB

Rb

Hu

45932

CD31

M

ICC/IF, IP, WB

Ms

Hu, Ms, Rat

24590

c-kit

P

IHC-P, WB, ELISA, ICC/IFRb

Hu, Ms

5506

c-kit

P

Flow Cyt, ICC/IF, IHC-P

Rb

Hu

16832

ETS1 antibody [1G11]

M

IHC-P, IP, WB

Ms

Hu

10936

GATA2 antibody

P

IHC-P, WB

Rb

Hu

22849

GATA4 (phospho S105) antibody

P

EMSA, WB

RbMs, Rat, Dog

5245

GATA4 antibody

P

ELISA, ICC/IF, WB

Rb

Hu, Rat

61767

HCN4 antibody

P

IHC-Fr, IHC-P, WB

Rb

Hu, Ms, Rat

66501

HCN4 antibody [SHG 1E5]

M

IHC-Fr, WB

Rt

Hu, Ms, Rat

32675

IRX4 antibody

P

ELISA, WB

Rb

Hu

56032

PICC, Immunohistochemistry (PFA fixed)

Rb

Ms, Chk

26122

MDR-1/P Glycoprotein

M

Flow Cyt, ICC/IF

Ms

Hu

10333

MEF2A antibody

P

ELISA, IHC-P, WB

RB

Hu, Ms, Rat

51153

MEF2C (phospho S387) antibody

PELISA, WB

Rb

Hu

65252

MEF2C antibody

PWB

Ms

Hu

43796

Mesp-1M

WB, ELISA

Ms

Hu

77013

Nkx2.5 antibody

P

IHC-P, WB

RbHu

22611

Nkx2.5 antibody

P

IHC-P, WB

RbHu

35842

Sca-1 (FITC-conjugated)

M

Flow Cyt, IP, WB, IHC-FR, CellAct

Rt

Ms

25031

Smad-1P

WB, ELISA, ICC/IFRb

Hu, Ms63356

Smad-1 (phospho S465)

P

IHC-P, WB, ELISA

Rb

Hu, Ms, Rat

63439

Islet 1 antibody Neural Stem Cell MarkerSee more products at: www.abcam.com

Featured antibodies

Heavy chain cardiac Myosin antibody [3-48] (ab15)
Clonality Applications
M
ELISA, Flow Cyt, ICC, ICC/IF,IHC-Fr, IHC-P, WB

HostMsSpecies cross reactivity
Hu, Ms, Rat, Cow,Dog, Pig, Rb

Host
Rb

Species cross reactivity
Hu, Ms, Rat

Gata6 antibody (ab22600)
Clonality Applications
PWB

alpha smooth muscle Actin antibody (ab5694)
Clonality ApplicationsP
ELISA, ICC/IF, IHC-FoFr, IHC-Fr,IHC-P, WB

HostRbSpecies cross reactivity
Hu, Ms, Rat, Chk,Cow, Pig

HostRbSpecies cross reactivity
Hu

HostRbSpecies cross reactivity
Hu, Ms, Rat

CD31 antibody (ab76533)
Clonality Applications
M
Flow Cyt, IHC-P, IP, WB

heavy chain 2 antibody

P

ICC/IF, IHC-Fr, WB

RbCow

53219

TGF beta 1 antibody [2Ar2]M

ELISA, Flow Cyt, ICC, IHC-Fr, IHC-P, Neut, WBMS

Hu, Ms, Rat

64715

VE Cadherin antibody

P

IHC-P, WB

Rb

Hu, Ms33168

Islet 1 antibody (ab20670)
Clonality ApplicationsP
ICC/IF, IF, IHC-Fr

VEGF Receptor 2 antibody P

Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB

Rb

Hu, Ms, Rat, Cow, Dog

2349

VEGF Receptor 2 antibody [KDR/EIC]Hemangioblast Marker

M

ELISA, Flow Cyt, ICC/IF, IHC-Fr

Ms

Hu, Rat

9530

Wnt1

P

IHC-P, WB

Rb

Hu, Ms, Rat

15251

005-01/09-PW

Smooth muscle Myosin