ANNUAL REPORTS
OF
INTERNATIONAL CENTER FOR BIOTECHNOLOGYOSAKA UNIVERSITY
VOL. 41, 2019
DIRECTOR/EDITORFUJIYAMA KAZUHITOASSISTANT EDITORHONDA KOHSUKEKITANI SHIGERUMISAKI RYO
KAJIURA HIROYUKI

SECRETARYMATSUMOTO REIKOTOMOMATSU FUMIKOYAMASHITA KEIKOKATAYAMA CHIYOKOKAWAKAMI SHIZUKAKIKUCHI TERUMIThe Annual Report is published to record the activity of the International
Center for Biotechnology (ICBiotech) and issued once in each fiscal year.
It contains scientific articles, progress reports, letters, and announcementfrom the Center. This volume includes publications by the former
participants in UNESCO courses. The editor welcomes the submission of
appropriate articles from all persons who are concerned with the activity of
the Center. All the contributions, however, will be reviewed by editors
before their acceptance. The scientific paper herein should be treated as
personal communications and not treated as original publications. The
Annual Report is distributed upon request to the International Center for
Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871,
Japan (e-mail: info_icbio@icb.osaka-u.ac.jp).

CONTENTS
The Neutral N-linked Glycans of the Basidiomycetous Yeasts Pseudozyma antarctica and Malassezia furfur(Subphylum Ustilaginomycotina)
R. J. D. Flores, T. Ohashi, K. Sakai, T. Gonoi, H. Kawasaki, and K. Fujiyama
Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluviali
S. Bunsangiam, V. Sakpuntoon, N. Srisuk, T. Ohashi, K. Fujiyama, and S. Limtong
List of Publications: FUJIYAMA Kazuhito
In vitro Production of Cysteine from Glucose
Y. Hanatani, M. Imura, H. Taniguchi, K. Okano, Y. Toya, R. Iwakiri, and K. Honda
Developing a Single Strain for in vitro Salvage Synthesis of NAD+ at High Temperatures and its Potential for
Bioconversion
H. Taniguchi, M. Imura, K. Okano, and K. Honda
List of Publications: HONDA Kohsuke
Biological Treatment of Selenate-Containing Saline Wastewater by Activated Sludge under Oxygen-Limiting
Conditions
Y.Y. Zhang, M. Kuroda, S. Arai, F. Kato, D. Inoue, and M. Ike
Enhanced Biomass Production and Nutrient Removal Capacity of Duckweed via Two-Step Cultivation Processwith A Plant Growth-Promoting Bacterium, Acinetobacter calcoaceticus P23
H. Ishizawa, Y. Ogata, Y. Hachiya, K. Tokura, M. Kuroda, D. Inoue, T. Toyama, Y. Tanaka, K. Mori,
M. Morikawa, and M. Ike
List of Publications: IKE Michihiko
A Conserved Strategy of Chalcone Isomerase-like Protein to Rectify Promiscuous Chalcone Synthase Specificity
T. Waki, R. Mameda, T. Nakano, S. Yamada, M. Terashita, K. Ito, N. Tenma, Y.B. Li, N. Fujino, K. Uno,
S. Yamashita, Y. Aoki, K. Denessiouk, Y. Kawai, S. Sugawara, K. Saito, K. Yonekura-Sakakibara, Y. Morita,
A. Hoshino, S. Takahashi, and T. Nakayama
Glycoside‐Specific Glycosyltransferases Catalyze Regio‐Selective Sequential Glucosylations for a Sesame
Lignan, Sesaminol Triglucoside.
E. Ono, T. Waki, D. Oikawa, J. Murata, A. Shiraishi, H. Toyonaga, M. Kato, N. Ogata, S. Takahashi,
M. Yamaguchi, M. Horikawa, and T. Nakayama
List of Publications: NAKAYAMA Toru
Heterologous Production of Coryneazolicin in Escherichia coli
M. Takuma, M. Kuroha, Y. Nagano, I. Kaweewan, H. Hemmi, T. Oyoshi, and S. Kodani
Isolation and Structure Determination of New Chymotrypsin Inhibitory Peptides Streptopeptolins
B and CA. Tiwari, I. Kaweewan, Y. Miyake, H. Hemmi, and S. Kodani
List of Publications: KODANI Shinya
Delta-9 Fatty Acid Desaturase Overexpression Enhanced Lipid Production and Oleic Acid Content inRhodosporidium toruloides for Preferable Yeast Lipid Production
Y.-Y. Tsai, T. Ohashi, C.-C. Wu, D. Bataa, R. Misaki, S. Limtong, and K. Fujiyama
The Neutral N-linked Glycans of the Basidiomycetous Yeasts Pseudozyma antarctica and Malassezia furfur(Subphylum Ustilaginomycotina)
R. J. D. Flores, T. Ohashi, K. Sakai, T. Gonoi, H. Kawasaki, and K. Fujiyama
List of Publications: OHASHI Takao

GC/MS Based Metabolite Profiling of Indonesian Specialty Coffee from Different Species and GeographicalOriginS. P. Putri, T. Irifune, Yusianto, and E. Fukusaki
Metabolome analysis revealed the knockout of glyoxylate shunt as an effective strategy for improvement of
1-butanol production in transgenic Escherichia coli
K. Nitta, W. A. Laviña, S. Pontrelli, J. C. Liao, S. P. Putri, and E. Fukusaki
List of Publications: FUKUSAKI Eiichiro
Parasite‐Mediated Selection in a Natural Metapopulation of Daphnia magna
A. P. Cabalzar, P. D. Fields, Y. Kato, H. Watanabe, and D. Ebert
Class III Polyphosphate Kinase 2 Enzymes Catalyze the Pyrophosphorylation of Adenosine-5’-Monophosphate
M. Ogawa, A. Uyeda, K. Harada, Y. Sato, Y. Kato, H. Watanabe, K. Honda, and T. Matsuura
List of Publications: WATANABE Hajime
Dataset of Microbial Community Structure in Alcohol Sprayed Banana Associated with Ripening Process
F. M. Dwivany, F. Syam, H. Nugrahapraja, O. K. Radjasa, M. R. Moeis, and S. Uchiyama
The Fab Portion of Immunoglobulin G Contributes to its Binding to Fcγ Rceptor III
R. Yogo, Y. Yamaguchi, H. Watanabe, H. Yagi, T. Satoh, M. Nakanishi, M. Onitsuka, T. Omasa, M. Shimada,T. Maruno, T. Torisu, S. Watanabe, D. Higo, T. Uchihashi, S. Yanaka, S. Uchiyama, and K. Kato
List of Publications: UCHIYAMA Susumu
Functional Specialization of UDP-glycosyltransferase 73P12 in Licorice to Produce a Sweet Triterpenoid Saponin,
Glycyrrhizin
Y. Nomura, H. Seki1, T. Suzuki, K. Ohyama, M. Mizutani, T. Kaku, K. Tamura, E. Ono, M. Horikawa, H. Sudo, H.
Hayashi, K. Saito, and T. Muranaka
Identification of Oxidosqualene Cyclases from the Medicinal Legume Tree Bauhinia forficata: a Step toward
Discovering Preponderant α-amyrin-Producing Activity
P. Srisawat, E. O. Fukushima, S. Yasumoto, J. Robertlee, H. Suzuki, H. Seki, and T. Muranaka
Designing a Blueprint for Next‐Generation Stem Cell Bioprocessing Developments
M.-H. Kim and M. Kino-oka
Maintenance of Neurogenic Differentiation Potential in Passaged Bone Marrow-Derived Human Mesenchymal
Stem Cells Under Simulated Microgravity Conditions
C. Koaykul, M.-H. Kim, Y. Kawahara, L. Yuge, and M. Kino-oka
List of Publications: KINO-OKA Masahiro
Characterization of Chinese Hamster Ovary Cells with Disparate Chromosome Numbers: Reduction of theAmount of mRNA Relative to Total Protein
N. Yamano-Adachi, N. Ogata, S. Tanaka, M. Onitsuka, and T. Omasa
Improvement of Intracellular Traffic System by Overexpression of KDEL Receptor 1 in Antibody-Producing CHO
Cells
A. Samy, K. Kaneyoshi, and T. Omasa
List of Publications: OMASA Takeshi
Programmable Artificial Cells Using Histamine-Responsive Synthetic Riboswitch
M. Dwidar, Y. Seike, S. Kobori, C. Whitaker, T. Matsuura, and Y. Yokobayashi
Bottom-up Creation of an Artificial Cell Covered with the Adhesive Bacterionanofiber Protein AtaAK. Noba, M. Ishikawa, A. Uyeda, T. Watanabe, T. Hohsaka, S. Yoshimoto, T. Matsuura, and K. HoriAnalysis and enhancement of the ethanol resistance of Pichia kudriavzevii N77-4, a strain newly isolated fromthe Korean traditional fermentation starter Nuruk, for improved fermentation performance
M. Sugiyama, T. Fukaya, J. Y. Moon, K. Ishida, A. Matsushita, S. Y. Kim, and S. H. Yeo
Identification of Biosynthetic Genes for the β‑Carboline Alkaloid Kitasetaline and Production of the FluorinatedDerivatives by Heterologous Expression
S. Ueda, H. Ikeda, T. Namba, Y. Ikejiri, Y. Nishimoto, M. Arai, T. Nihira, and S. Kitani

PUBLICATIONS by Collaborative Professors at Osaka University
In vitro Antiviral Activity of Spirotetronate Compounds Against Dengue Virus Serotype 2
J. Euanorasetr, B. Intra, N. Thunmrongsiri, J. Limthongkul, S. Ubol, A. Anuegoonpipat, T. Kurosu, K. Ikuta,
T. Nihira, and W. Panbangred
Antibacterial Activity of Sustainable Composites Derived from Epoxidized Natural Rubber/Silver-Substituted
Zeolite/Poly(Lactic acid) Blends
P. Taranamai, P. Phinyocheep, W. Panbangred, M. Janhom, and P. Daniel
Efficacy of Liquid Bacterial Formulation of Bacillus thuringiensis subsp. israelensis and Bacillus sphaericus
1593 Mixture in Controlling Mosquito Larvae in Bangkok
S. Prummongkol, N. Komalamisra, T. Phanphoowong, S. Chauvatcharin, W. Panbangred, and R. Srisawat

PUBLICATIONS by Collaborative Researchers
Effect of Tarcolimus on Skin Microbiome in Atopic Dermatitis
J. Wongpiyabovorn, W. Soonthornchai, A. Wilantho, M. Palasuk, S. Payungporn, P. Sodsai, W. Poomipak,
S. Weschawalit, K. Ruchusatsawat, G.S. Baillie, N. Hirankarn, and N. Somboonna
Marine Bacterial Diversity in Coastal Sichang Island, the Upper Gulf of Thailand, in 2011 Wet Season
N. Somboonna, A. Wilantho, P. Rerngsamran, and S. Tongsima
List of Publications: Naraporn Somboonna
Bioaugmentation Coupled with Phytoremediation for the Removal of Phenolic Compounds and Color from
Treated Palm Oil Mill Effluent
P. Jarujareet, K. Nakkanong, E. Luepromchai, and O. Suttinun
Inhibitory Effect of Phenol on Wastewater Ammonification
C. Rongsayamanont, P. Khongkhaem, E. Luepromchai, and E. Khan
Detection of African Swine Fever Virus in Pork Products Brought to Taiwan by Travellers
W.-H. Wang, C.-Y. Lin, M. R. C. Ishcol, A. N. Urbina, W. Assavalapsakul, A. Thitithanyanont, P.-L. Lu,
Y.-H. Chen, and S.-F. Wang
Imported Dengue Fever and Climatic Variation are Important Determinants Facilitating Dengue Epidemics inSouthern Taiwan
W.-H. Wang, H.-J. Chen, C.-Y. Lin, W. Assavalapsakul, and S.-F. Wang
List of Publications: Wanchai Assavalapsakul
Complete Genome Sequence of Halocella sp. Strain SP3-1, an Extremely Halophilic, Glycoside Hydrolase- andBacteriocin-Producing Bacterium Isolated from a Salt Evaporation Pond
S. Heng, S. Sutheeworapong, P. Prommeenate, S. Cheevadhanarak, A. Kosugi, P. Pason, R. Waeonukul,
K. Ratanakhanokchai, and C. Tachaapaikoon
Characterization of Lignocellulose-degrading Thermophilic Anaerobic Bacterium, Caldicellulosiruptor sp. strain
EP2: its Properties and Genome
P. Tongsuk, S. Heng, S. Sutheeworapong, P. Prommeenate, A. Kosugi, P. Pason, R. Waeonukul,
K. Ratanakhanokchai, and C. Tachaapaikoon
List of Publications: Chakrit Tachaapaikoon

PTM-Logo: a Program for Generation of Sequence Logos Based on Position-Specific Background Amino-acid
Probabilities
T. Saethang, K. Hodge, C.-R. Yang, Y. Zhao, I. Kimkong, M. A. Knepper, and T. Pisitkun
Amycolatopsis acidicola sp. nov., Isolated from Peat Swamp Forest Soil
W. F. A. Teo, N. Srisuk, and K. Duangmal
Gordonia oryzae sp. nov., Isolated from Stems of Rice Plant (Oryza sativa L.)
S. Muangham, K. Lipun, A. Thamchaipenet, A. Matsumoto, and K. Duangmal
List of Publications: Kannika Duangmal
Micromonospora pelagivivens sp. nov., a New Species of the Genus Micromonospora Isolated from Deep-Sea
Sediment in Japan
B. Intra, W. Panbangred, Y. Inahashi, A. Také, M. Mori, S. Ōmura, and A. Matsumoto
Saccharopolyspora rhizosphaerae sp. nov., an actinomycete isolated from rhizosphere soil in Thailand
B. Intra, J. Euanorasetr, A. Také, Y. Inahashi, M. Mori, W. Panbangred, and A. Matsumoto
List of Publications: Bungonsiri Intra

PUBLICATIONS by Former Participants in
UNESCO International Post-Graduate University Course in Microbiology (UM),UNESCO Postgraduate Inter-University Course in Biotechnology (UB),and UNESCO Training Project supported by ODA Grants for UNESCO Activities, MEXT (UO)
Evidence of Biocontrol Activity of Bioinoculants Against a Human Pathogen, Listeria monocytogenes
R. Sharma, L. Gal, D. Garmyn, V. S. Bisaria, S. Sharma, and P. Piveteau
Impact of Abiotic Stressors on Native Rhizospheric Bacterial Community of Cajanus cajan
G. Anand, V. S. Bisaria, and S. Sharma
Magnetic Cross-Linked Enzyme Aggregates of Aspergillus oryzae ST11 Lipase Using Polyacrylonitrile Coated
Magnetic Nanoparticles for Biodiesel Production
P. Paitaid and A. H-Kittikun
Pentamycin Biosynthesis in Philippine Streptomyces sp. S816: Cytochrome P450-Catalyzed Installation of the
C‑14 Hydroxyl GroupS. Zhou, L. Song, J. Masschelein, F. A. M. Sumang, I. A. Papa, T. O. Zulaybar, A. B. Custodio, D. Zabala,E. P. Alcantara, E. L. C. de los Santos, and G. L. Challis
Symbiotic Properties of a Chimeric Nod-independent Photosynthetic Bradyrhizobium Strain Obtained by
Conjugative Transfer of a Symbiotic Plasmid
P. Songwattana, P. Tittabutr, J. Wongdee, K. Teamtisong, D. Wulandari, A. Teulet, J. Fardoux, N. Boonkerd,
E. Giraud, and N. Teaumroong
Co-Inoculation of Bacillus velezensis Strain S141 and Bradyrhizobium Strains Promotes Nodule Growth andNitrogen Fixation
S. Sibponkrung, T. Kondo, K. Tanaka, P. Tittabutr, N. Boonkerd, K. Yoshida, and N. Teaumroong
List of Publications: Neung Teaumroong
A Novel Multifunctional GH9 Enzyme from Paenibacillus curdlanolyticus B-6 Exhibiting endo/exo Functionsof Cellulase, Mannanase and Xylanase Activities
V. Phakeenuya, K. Ratanakhanokchai, A. Kosugi, and C. Tachaapaikoon
Molecular Characterization of Hypothetical Scaffolding-like Protein S1 in Multienzyme Complex Produced by
Paenibacillus curdlanolyticus B-6
P. Pason, J. Sermsathanaswadi, R. Waeonukul, C. Tachaapaikoon, S. Baramee, K. Ratanakhanokchai, andA. Kosugi

List of Publications: Khanok Ratanakhanokchai
Characterization of a Novel Clostridium sp. SP17-B1 and its Application for Succinic Acid Production from
Hevea Wood Waste Hydrolysate
S. Phuengjayaem, S. Tanasupawat, and S. Teeradakorn
Improved Pullulan Production by a Mutant of Aureobasidium melanogenum TN3-1 from a Natural Honey andCapsule Shell Preparation
L. Chen, Z. Chi, G.-L. Liu, S.-J. Xue, Z.-P. Wang, Z. Hu, and Z.-M. Chi
Metabolic Rewiring Improves the Production of the Fungal Active Targeting Molecule Fusarinine C
Y. Lu, H. Wang, Z. Wang, Y. Cong, P. Zhang, G. Liu, C. Liu, Z.-M. Chi, and Z. Chi
List of Publications: Zhen-Ming Chi
Novel Penicillium Species Causing Disseminated Disease in a Labrador Retriever Dog
T. Rothacker, J. A. Jaffey, E. R. Rogers, W. H. Fales, C. F. C. Gibas, N. P. Wiederhold, C. Sanders, J. Mele,H. Fan, L. A. Cohn, and A. RoyalA Novel Exophiala Species Associated with Disseminated Granulomatous Inflammation in a Captive Eastern
Hellbender (Cryptobranchus alleganiensis alleganiensis)
C. Hopf, E. A. Graham, C. F. C. Gibas, C. Sanders, J. Mele, H. Fan, M. M. Garner, N. P. Wiederhold,
R. Ossiboff, and N. Abou-Madi
List of Publications: Connie F. C. Gibas
Design of a Programmable Biosensor-CRISPRi Genetic Circuits for Dynamic and Autonomous Dual-Control of
Metabolic Flux in Bacillus Subtilis
Y. Wu, T. Chen, Y. Liu, R. Tian, X. Lv, J. Li, G. Du, J. Chen, R. Ledesma-Amaro, and L. Liu
Synthetic N-terminal Coding Sequences for Fine-Tuning Gene Expression and Metabolic Engineering in
Bacillus subtilis
R. Tian, Y. Liu, J. R. Chen, J. Li, L. Liu, G. Du, and J. Chen
List of Publications: Jian Chen
Characterization of TK1646, a Highly Thermostable 3′–5′ Single Strand Specific Exonuclease from
Thermococcus kodakarensis
M. S. Saeed and N. Rashid
A Highly Stable Manganese Catalase from Geobacillus thermopakistaniensis: Molecular Cloning andCharacterization
A. Shaeer, M. Aslam, and N. Rashid
List of Publications: Naeem Rashid
Bacillus amyloliquefaciens, a Narra (Pterocarpus indicus) Leaf Endophyte, Possesses Antifungal Activity
against Phytopathogenic Fungi due to Laminarinase Activity and Production of Antimicrobial Compounds
R. H. Parcon, M. D. E. Isagan, L. D. C. Atole, V. A. Alcantara, and J. F. Simbahan
Yeast and Bacterial Community Profiling of Fermenting Nipa (Nypa fruticans) Sap in Two Philippine Locations
and Fermentation Characteristics of Selected Yeasts
J. P. T. Madigal, J. F. Simbahan, N. B. Lantican, S. Agrupis, and F. B. Elegado
Occurrence of Diversified N‐acyl homoserine Lactone Mediated Biofilm‐Forming Bacteria in Rice
Rhizoplane
B. Viveka and D. Balachandar
Source Tracking of Shiga-Like Toxin-Producing Escherichia coli in the Fresh Vegetable Production System ofSouth India
P. Pushpakanth, Z. J. Kennedy, and D. Balachandar

List of Publications: Dananjeyan Balachandar
Clinical Significance of Circulating Soluble Immune Checkpoint Proteins in Sorafenib-treated Patients with
Advanced Hepatocellular Carcinoma
M. P. Dong, M. Enomoto, L. T. T. Thuy, H. Hai, V. N. Hieu, D. V. Hoang, A. Iida-Ueno, N. Odagiri,
Y. Amano-Teranishi, A. Hagihara, H. Fujii, S. Uchida-Kobayashi, A. Tamori, and N. Kawada
Intention-to-treat Assessment of Glecaprevir + Pibrentasvir Combination Therapy for Patients with Chronic
Hepatitis C in the Real World
A. Tamori, K. Inoue, T. Kagawa, K. Takaguchi, K. Nouso, Y. Iwasaki, M. Minami, H. Hai, M. Enomoto, and
N. Kawada
Development and Characterisation of Active Antioxidant Packaging Films
D. L. Belan, L. E. Mopera, and F. P. Flores
Novel Analytical Platform For Robust Identification of Cell Migration Inhibitors
P. Somchai, K. Phongkitkarun, P. Kueanjinda, S. Jamnongsong, K. Vaeteewoottacharn, V. Luvira,S. Okada, S. Jirawatnotai, and S. Sampattavanich
Profile of Histone H3 Lysine 4 Trimethylation and the Effect of Lipopolysaccharide/Immune ComplexActivated Macrophages on Endotoxemia
V. Ruenjaiman, P. Butta, Y.-W. Leu, M. Pongpanich, A. Leelahavanichkul, P. Kueanjinda, and T. Palaga
Cadherin 11 Inhibition Downregulates β-catenin, Deactivates the Canonical WNT Signalling Pathway andSuppresses the Cancer Stem Cell-Like Phenotype of Triple Negative Breast Cancer
P. B. Satriyo, O. A. Bamodu, J.-H. Chen, T. Aryandono, S. M. Haryana, C.-T. Yeh, and T.-Y. Chao
Upregulation of Circulating MiR-21 Expression as a Potential Biomarker for Therapeutic Monitoring andClinical Outcome in Breast Cancer
S. L. Anwar, D. N. I. Sari, A. I. Kartika, M. S. Fitria, D. S. Tanjung, D. Rakhmina, T. Wardana, I. Astuti,
S. M. Haryana, and T. Aryandono
List of Publications: Sofia Mubarika Haryana
Homocitrate Synthase Genes of Two Wide-Host-Range Bradyrhizobium Strains are Differently Required for
Symbiosis Depending on Host Plants
S. Hashimoto, J. Wongdee, P. Songwattana, T. Greetatorn, K. Goto, P. Tittabutr, N. Boonkerd,
N. Teaumroong, and T. Uchiumi
List of Publications: Panlada Tittabutr
Profile of Microflora “Jambal Roti” (Traditional Fermented Fish) with Pediococcus sp (Pediococcus acidilactici
F11 and Pediococcus halophillus FNCC-0032) Aplication and 25% NaCl
M. Karyantina, S. Anggrahini, T. Utami, and E. S. Rahayu
List of Publications: Merkuria Karyantina
Activities of International Center for Biotechnology for 2019
Author Index

ACTIVITIES OF ICBiotech

ACTIVITIES OF INTERNATIONAL CENTER FOR
BIOTECHNOLOGY FOR FY 2019
The International Center for Biotechnology (ICBiotech) was founded in April 1995 as anindependent institute in Osaka University with a mission to pursue academic advancement
and collaborative research in biotechnology. ICBiotech has its origin from the “International
Center of Cooperative Research Center in Microbial Engineering Japan (ICME)” which wasestablished in the Faculty of Engineering, Osaka University in April 1978, through renaming
to “International Center of Cooperative Research in Biotechnology (ICBiotech)” in April
1985 with the recognition of the wide acceptance and success of ICME’s activities andachievements.
ICBiotech is dedicated to promote international cooperation among Asian countries in the
aspects of research and educational advancement in the field of Biotechnology by propelling
academic interactions in Asian countries, and is committed to industrial biotechnology studies
by means of microbial engineering and related sciences, focusing on the sustainableutilization of abundant natural resources in Southeast Asian countries.
ICBiotech serves as the seat of education and research in Asia, with the support of the
Ministry of Education, Culture, Sports, Science and Technology (Monbu-kagaku-sho,
MEXT), the Japan Science and Technology Agency (JST), the Japan Student Service
Organization (JASSO) and the Japan International Cooperation Agency (JICA), in
cooperation with the Department of Biotechnology, Graduate School of Engineering, Osaka
University, as well as researchers from prestigious universities nationwide and abroad.
In 2002 Cooperative Research Station (CRS) in Southeast Asia and Mahidol
University-Osaka University Collaborative Research Center for Bioscience andBiotechnology (MU-OU:CRC) were set up at Faculty of Science, Mahidol University in
Thailand as a collaborative research center to accomplish multidisciplinary research in thefield of Bioscience and Biotechnology.
The activities of the ICBiotech include:
1. Research and Education
The main area of research is industrial biotechnology rooted in microbial engineering,whilst centering on the sustainable use of agricultural and forest resources in bioresourcerich countries such as those in Southeast Asia. Research is underway in the field of cellengineering with the objectives of analyzing the cellular functions of bacteria, fungi andplants, and developing and using functions of these cells for management and rational useof biological resources that exist on our planet. ICBiotech covers three areas of Biotechnology:
1) Discovery of new functions from biological resources.
2) Bio-conversion and process engineering of biological resources.
3) Conservation of biological resources.
For under graduate and post graduate courses, ICBiotech is involved in the education
activities of Department of Biotechnology, Graduate School of Engineering as collaborating laboratories and currently covers several fields of biotechnology in education andresearch: Applied Microbiology Laboratory chaired by Prof. FUJIYAMA Kazuhito andMolecular Microbiology laboratory chaired by Prof. HONDA Kohsuke.
2. Participating in FrontierLab@OsakaU ’Scientific Empowerment Program for International Students’ which was created for international students to conduct thematicstudies and achieve results under the guidance of supervisors while acquiring skills
necessary for continuing research in one of Osaka University’s internationally renowned
science and technology laboratories for a period of up to 12 months.
3.

Acting as collaborating laboratories with Department of Biotechnology, Graduate Schoolof Engineering, Osaka University for ‘Biotechnology Global Human Resource
Development Program for Industry-University Co-Creation’. The aim of this program is
to expose graduate students (privately financed as well as the Japanese Government
Scholarship students) to state-of-the-art research skills and in-depth knowledge ofadvanced biology to harness the potential of biotechnology.

4. Having been administrating and implementing the “UNESCO Biotechnology School inAsia” (2012-2016) at Department of Biotechnology, Graduate School of Engineering,Osaka University, taking over the long-termed “International Post-Graduate University
Course in Microbiology” (1972-2003) and Postgraduate Inter-University Course in
Biotechnology (2004-2007) with total of 459 alumni spreading all over Asia.
5. Serving as the secretariat for the UNESCO Regional Network for Microbiology andBiotechnology in Southeast Asia in accordance with a proposed memorandum resulting
from a Consortium Meeting of the Project “Tropical Bio-Resources and Green Chemistry
Strategy” (Osaka 2003).
6. Working as a member of the global network “Microbiological Resource Centers”(MIRCENs) operated by UNEP, UNESCO and ICRO; and as an original member of the“Regional Network for Microbiology in Southeast Asia” operated under the auspices of
UNESCO through the Fund-In-Trust of the Japanese Government (J-FIT).
7.

Promoting international cooperative researches in biotechnology with the Southeast
Asian countries related to Biotechnology, under the support of Monbu-kagaku-sho
Grant-in-Aids for International Scientific Research. In addition, ICBiotech cooperates indeveloping international organization and conducting academic seminars related tobiotechnology.

8.

Implementing Student Exchange Support Program with the support of JASSO. Under the
program, graduate students of Osaka University are sent to Thai four universities for afield study program named “Bio-resource & environment”, and graduate students ofThailand and ASEAN countries are invited to Osaka University for lab study programs
named “Bio-industry & bio-diversity” as well as “ASEAN Biotechnology School”, all forabout 5 weeks.

9.

Inviting Asian students through Sakura Science Plan (SAKURA SCIENCE Exchange

Program) of JST to introduce and offer experiences in Japanese science and techonology.
By exchanging ideas among the participants, the Plan aims to support the development of
talented people overseas who have the potential to contribute to innovation in science andtechnology and support continuous interaction between Japan and other countries; topromote globalization of Japanese education and research institutes; to strengthen good
relationship between Japan and other countries.
10. Accepting graduate students of JICA partner schools in Asian countries to provide aninternship with the support of JICA Innovative Asia Program that aims to enhance thecirculation of capable young personnel between Japan and Asian countries and topromote innovation in the whole Asia.
11. Implementing Plant Biotech Program with the University of California, Davis, thatenhances cooperation between the two universities to promote healthy and sustainable
planet by exploring the intersectionality of biology and engineering.
12. Promoting ASEAN Campus Project organized by Osaka University that aims at
contributing to “Quality Growth” and the development of high-level global humanresources for the next generation in ASEAN countries and Japan.
13. Periodical publishing of Annual Reports of ICBiotech.

STEERING COMMITTEE
ChairmanCommittee Members

Prof. FUJIYAMA Kazuhito (Director of ICBiotech)
Prof. HONDA Kohsuke (International Center for Biotechnology)
Prof. FUKUSAKI Eiichiro (Graduate School of Engineering)
Prof. WATANABE Hajime (Graduate School of Engineering)
Prof. TOBISU Mamoru (Graduate School of Engineering)
Prof. HIRATA Kazumasa (Graduate School of Pharmaceutical Sciences)Prof. UMAKOSHI Hiroshi (Graduate School of Engineering Science)Prof. IIDA Tetsuya (Research Institute for Microbial Diseases)
Prof. KURISU Genji (Institute for Protein Research)

STAFF
Director/Professor
Professor

Dr. FUJIYAMA Kazuhito
Dr. HONDA Kohsuke

Adjunct Professor

Dr. IKE MichihikoCollaborative Professor(Division of Sustainable Energy and Environmental Engineering,
Graduate School of Engineering, Osaka University)
Dr. Watanalai PANBANGRED (Mahidol University, Thailand)

Dr. Irfan Dwidya Prijambada(Universitas Gadjah Mada, Indonesia)

Dr. Raymond L. RODRIGUEZ(University of California-Davis, USA)

Guest Professor

Dr. YOSHIDA Toshiomi (Prof. Emer., Osaka University)
Dr. SEKI Tatsuji (Prof. Emer., Osaka University)
Mr. TAYAMA Junji (Osaka University)
Dr. NAKAYAMA Toru (Tohoku University)
Associate Professor
Dr. KITANI Shigeru
Dr. MISAKI Ryo
Adjunct Associate Professor Dr. SUMIMURA Yoshinori(Center for Global Initiatives, Osaka University)

Guest Associate Professor
Assistant Professor
Visiting Academic Staff

Dr. KODANI Shinya (Shizuoka University)
Dr. OHASHI Takao (Setsunan University)
Dr. KAJIURA Hiroyuki
Dr. Suchada Chanprateep NAPATHORN(Associate Professor, Fac. of Science, Chulalongkorn University,
Thailand) Specially Appointed Associate Professor under
the Cross-appointment Agreement

Dr. Dian Anggraini SUROTO(Assistant Professor, Fac. of Agricultural Technology, Gadjah Mada
University, Indonesia) Specially Appointed Assistant Professor under
the Cross-appointment Agreement

Administrative Official
Administrative AssistantTechnical Assistant

Ms. MATSUMOTO Reiko
Ms. TOMOMATSU Fumiko
Ms. KATAYAMA Chiyoko
Ms. YAMASHITA KeikoMs. KAWAKAMI Shizuka
Ms. KIKUCHI Terumi

I. COOPERATIVE RESEARCH STATION (CRS) IN SOUTHEAST ASIA
The ICBiotech, Osaka University launched out the Cooperative Research Station (CRS) in
Southeast Asia at Chalermprakiat Building, Faculty of Science, Mahidol University in 2002
through the generous support by Mahidol University. The CRS's space and equipments are
made available for Southeast Asian and Japanese researchers to undertake cooperative onsite
researches on the development of the abundant natural biological and genetic resources andtheir sustainable utilization in Southeast Asian countries through JSPS core university
program (ended in 2005), JST Special Coordination Funds for Promoting Science andTechnology (2006-2009) and JSPS Asian CORE Program (2009-2014). The CRS alsofunctioned as a lecturing and research station of the UNESCO International Post-graduate
Inter-University program, which Osaka University operated in coordination with Thai
universities. The CRS is considering support to the alumni of Osaka University and provisionof university information for recruitment of students for study in Osaka University.
Mahidol University (MU) and Osaka University (OU) together established the Mahidol
University-Osaka University Collaborative Research Center (MU-OU:CRC) for Bioscienceand Biotechnology at Faculty of Science, Mahidol University in 2002, to strengthen the
research cooperation in these fields which are amongst the most active fields of study andresearch in both universities.
Currently, MU-OU:CRC has coordinated a research projects under the jointly support ofNational Research Council of Thailand (NRCT), National Center for Genetic Engineering andBiotechnology (BIOTEC) and The Japan Society for the Promotion of Science (JSPS).
Researchers from Mahidol University, Chulalongkorn University, Kasetsart University, King
Mongkut's University of Technology Thonburi and BIOTEC participate in this project.
CRS is conducting researches on:
1. Degrading enzymes.
2. Cell culture system for screening of bioactive compounds from Streptomycetes andenvironmental fungi.
3. Quorum sensing interference: Novel biocontrol strategies for pathogenic bacteria.
In addition to above, CRS has been taking care of graduate students of Osaka University
sent to Thai universities and Thai graduate students sent to Osaka University under the
JASSO Student Exchange Support Program.

II. WORKSHOPThe 9th Workshop on Collaboration
between Osaka University and Japanese companies
for global human resource development in the field of biotechnology

February 7, 2020
International Center for Biotechnology, Osaka University
Sponsorship; International Center for Biotechnology, Osaka University
Co-sponsored; International Research Initiative for Frontier of Industrial Biotechnology, Osaka University

The ninth Workshop on Collaboration between Osaka University and Japanese companies
was held. Representatives of industries engaged in the biotechnology industry wereintroduced to the international students enrolled at Osaka University for the purpose of
attaining international human resource development. A total of eight enthusiastic companies
attended the meeting. Companies, faculty members and students introduced themselves andtheir activities to one another. Students were able to know about the Japanese companies andhow to apply their scholarly works in the industrial biotechnology setting. Adding to the great
success of this meeting are the lively discussions and exchange meetings which have resulted
in a deepened understanding of each other.
13:30

Opening remark by Prof. FUJIYAMA Kazuhito

13:35-14:50

Special lecture by former international students of Osaka University,
who are successful in Japanese private companies.
Ahmad M. HAREDY (Biken, Osaka University)
Marliza MADUNG (Sysmex Corporation)

15:00-15:20

Keynote Address by Prof. Emer. YOSHIDA Toshiomi

15:30-17:30

Group talking with individual company(30 min each, three rounds at α building of ICBiotech)
Attending companies are:
Ezaki Glico Co., Ltd.
Kao Corporation
Glico Nutrition Co., Ltd.
Saraya Co., Ltd.
Sysmex Corporation
SHIMADZU CORPORATIONTamanoi Vinegar Co., Ltd.
Lion Corporation

17:45-19:30

Simple party with light meal at International Saloon

III. JASSO STUDENT EXCHANGE SUPPORT PROGRAM
Scholarship for Short Stay/ Short Visit Program (SSSV)This is a field study program jointly operated with several universities in Thailand and 3
ASEAN countries. In 2019, 22 first year students of the master's course of Osaka University
visited 4 universities in Thailand between August 4 and September 7, and 24 postgraduate
students from Thailand Philippines, Vietnam and Indonesia visited Osaka University between
November 5 and December 10, 2019, which enhanced mutual interactions.

IV. JST JAPAN-ASIA YOUTH EXCHANGE PROGRAM IN SCIENCE(SAKURA Exchange Program in Science)
Purpose of the Program: Promoting science and technology is a key engine to materializea bright future of Asia and it is vitally important to enhance the exchange of youths in Asian
countries and Japan who will play a crucial role in the field of science and technology. Basedon this concept, “Japan-Asia Youth Exchange Program in Science” (SAKURA Exchange
Program in Science) is the program for enhancing exchanges between Asia and Japan of the
youths who will play a crucial role in the future field of science and technology through theclose collaboration of industry-academia-government by facilitating short-term visits ofcompetent Asian youths to Japan. This program aims at raising the interest of Asian youths
toward the leading Japanese science and technologies at Japanese universities, research
institutions and private companies.

V. SCIENTIST EXCHANGES
Record of Scientist Exchange (FY2019)
From ICBiotech to counterpart countries / From counterpart countries to ICBiotech*Please contact us for more information.

IV. GUESTS/VISITORS*Please contact us for more information.

VII. STEERING COMMITTEE MEETING 2019
Steering Committee Meetings of ICBiotech were convened as follows:・May 13, 2019:

Discussion on establishment professor nomination committee andselection of committee members

・July 24, 2019:

Discussion on employment of an assistant professor of Applied
Microbiology Laboratory
Dr. KAJIURA Hiroyuki (September 1, 2019- )・August 27, 2019:

Discussion on:*Concluding a cross appointment agreement to employ a full-time
specially appointed assistant professor
Dr. Dian Anggraini SUROTO (January 20, 2020 - March 19,
2020)*Accepting a Visiting Researcher
Ms. NGUYEN Thi Sam (October 1, 2019 - March 30, 2020)*Partial revision of Regulations of Harassment Prevention Committeeof Graduate School of Engineering aimed at joined establishment of
the Committee with ICBiotech.

・October 3, 2019:

Discussion on:*Employment of the professor of the Laboratory of Molecular
Microbiology
Dr. HONDA Kohsuke (November 16, 2019 -)*Concluding MOU: Research Centre for Bio-molecular Engineering,
Universitas Airlangga, Indonesia.
*Accepting a Visiting Academic Staff
Dr. Usa BOONYUEN (November 10, 2019 - November 30, 2019)

・October 21, 2019:

Discussion on the change of accepting term of the Visiting Academic
Staff
Dr. Usa BOONYUEN (November 10, 2019 - December 9, 2019)

・December 13, 2019: Report on the installation of Steering Committee member,Prof. HONDA Kohsuke
Discussion on:*Replacement of an Evaluation Committee member from
Assoc. Prof. KITANI Shigeru to Prof. HONDA Kohsuke as ofJanuary 1, 2020)*Accepting Japan International Cooperation Agency Trainees
Natsorn WATCHARADULYARAT (January 6, 2020 – February
3, 2020)
Pornpimon TONGSUK (January 6, 2020 - February 3, 2020)*Concluding MOU on Student Exchange: Research Centre for
Bio-molecular Engineering, Universitas Airlangga, Indonesia*Concluding MOU: Faculty of Engineering, Kasetsart University,
Thailand・February 13, 2020: Report on:*JASSO Student Exchange Support Program (Short stay and Shortvisit)*JST Sakura Exchange Program in Science*JICA Innovative Asia

*FrontierLab Mini2*The 9th Workshop on Collaboration between Osaka University andJapanese companies for global human resource development in thefield of biotechnology*Adjunct professors*Short term training program, 2020
*Other topics:① Organization of ICBiotech② Evaluation Committee
Discussion on:*Employment of an assistant professor of the Laboratory of Molecular
Microbiology
Dr. OKANO Kenji (April 1, 2020 -)*Conferring the title of Collaborative Professors from abroad for
FY2020Prof. Watanalai PANBANGRED
Prof. Irfan Dwidya Prijambada
Emer. Prof. Raymond L. RODRIGUEZ*Inviting Visiting Professors from Japanese universities for FY2020
Emer. Prof. YOSHIDA Toshiomi
Emer. Prof. SEKI Tatsuji
Mr. TAYAMA Junji
Prof. UMENO Daisuke
Assoc.Prof. KOJIMA Nobuhiko
Assoc.Prof. OHASHI Takao
Assoc.Prof. YUMIOKA Hitomi