ANNUAL REPORTS
OF
INTERNATIONAL CENTER FOR BIOTECHNOLOGYOSAKA UNIVERSITY
VOL. 39, 2017

DIRECTOR/EDITOR

KAZUHITO FUJIYAMAASSISTANT EDITORTAKUYA NIHIRASHIGERU KITANIRYO MISAKIHIROSHI KINOSHITATAKAO OHASHISECRETARY

MUTSUKO TSUJI
FUMIKO TOMOMATSUCHIYOKO KATAYAMASHIZUKA KAWAKAMI
KYOKO SUGIMOTO

The Annual Report is published to record the activity of the International
Center for Biotechnology (ICBiotech) and issued once in each fiscal year.
It contains scientific articles, progress reports, letters, and announcementfrom the Center. This volume includes publications by the former
participants in UNESCO courses. The editor welcomes the submission of
appropriate articles from all persons who are concerned with the activity of
the Center. All the contributions, however, will be reviewed by editors
before their acceptance. The scientific paper herein should be treated as
personal communications and not treated as original publications. The
Annual Report is distributed upon request to the International Center for
Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871,
Japan (e-mail: info_icbio@icb.osaka-u.ac.jp).

1

CONTENTS
The Neutral N-linked Glycans of the Ustilaginomycete Yeast Sympodiomycopsis paphiopedili
R. J. D. Flores, T. Ohashi, H. Kawasaki, and K. Fujiyama

1

Development of a Sufficient and Effective Procedure for Transformation of an Oleaginous Yeast,
Rhodosporidium toruloides DMKU3-TK16
Y. Y. Tsai, T. Ohashi, T. Kanazawa, P. Polburee, R. Misaki, S. Limtong, and K. Fujiyama

14

List of Publications: Kazuhito Fujiyama

15

Characterization of the Biosynthetic Gene Cluster for Cryptic Phthoxazolin A in Streptomyces avermitilis D. A.
Suroto, S. Kitani, M. Arai, H. Ikeda, and T. Nihira

16

Discovering Potential Streptomyces Hormone Producers by Using Disruptants of Essential Biosynthetic Genes
as Indicator Strains
N. B. Thao, S. Kitani, H. Nitta, T. Tomioka, and T. Nihira

33

List of Publications: Takuya Nihira

34

Differential Oxidative and Antioxidative Response of Duckweed Lemna minor toward Plant Growth
Promoting/inhibiting Bacteria
H. Ishizawa, M. Kuroda, M. Morikawa, and M. Ike

35

Effect of Extracellular Electron Shuttles on Arsenic-mobilizing Activities in Soil Microbial Communities
S. Yamamura, T. Sudo, M. Watanabe, S. Tsuboi, S. Soda, M. Ike, and S. Amachi

42

List of Publications: Michihiko Ike

43

Iriomoteolides-9a and 11a: Two New Odd-numbered Macrolides from the Marine Dinoflagellate
Amphidinium Species
K. Kumagai, Masayuki Tsuda, E. Fukushi, J. Kawabata, A. Masuda, and Masashi Tsuda

44

Total Synthesis and Stereochemical Revision of Iriomoteolide-2a
K. Sakamoto, A. Hakamata, Masashi Tsuda, and H. Fuwa

51

Mechanism Underlying Rapid Responses to Boron Deprivation in Arabidopsis Roots
M. Kobayashi, M. Miyamoto, T. Matoh, S. Kitajima, S. Hanano, I. N. Sumerta, T. Narise, H. Suzuki,
N. Sakurai, and D. Shibata

52

Immunocytochemical Detection of Rhamnogalacturonan II on Forming Cell Plates in Cultured Tobacco Cells
Y. Zhou, T. Awano, M. Kobayashi, T. Matoh, and K. Takabe

62

List of Publications: Masaru Kobayashi

62

PUBLICATIONS by Visiting Professors and Research Scholar at Osaka University
Saccharomonospora colocasiae sp. nov., an Actinomycete Isolated from the Rhizosphere of Colocasia esculenta
W. Wattanasuepsin, B. Intra, A. Take, Y. Inahashi, J. Euanorasetr, S. Omura, A. Matsumoto,63
and W. Panbangred
Improvement of Extracellular Bacterial Protein Production in Pichia pastoris
by Co-expression of Endoplasmic Reticulum Residing GroEL－GroES
P. Summpunn, J. Jomrit, and W. Panbangred

69

List of Publications: Watanalai Panbangred

70iSimultaneous Hydrolysis and Fermentation of Sweet Sorghum Varieties (FS501 and KCS105) into Bioethanol
Using Saccharomyces steineri – A Kinetics Study
H. Sudibyo, Jasman, H. T. B. M. Petrus, A. Prasetya, D. Widianto, C. Hidayat, S. Harashima,and I. D. Prijambada

71

Gene Polymorphisms of Autophagy Machinery and the Risk of Hepatitis B Virus-related Hepatocellular
Carcinoma in a Thai Population
S. Wisetsathorn, V. Tantithavorn, N. Hirankarn, P. Tangkijvanich, T. Saethang, and I. Kimkong

88

AbDesigner3D: a Structure-guided Tool for Peptide-based Antibody Production
T. Saethang, K. Hodge, I. Kimkong, D. M. Payne, M. A. Knepper, and T. Pisitkun

95

PUBLICATIONS by Collaborative Researchers
Chemical Pretreatment-Independent Saccharifications of Xylan and Cellulose of Rice Straw
by Bacterial Weak Lignin-Binding Xylanolytic and Cellulolytic Enzymes
T. Teeravivattanakit, S. Baramee, P. Phitsuwan, S. Sornyotha, R. Waeonukul, P. Pason, C. Tachaapaikoon,
K. Poomputsa, A. Kosugi, K. Sakka, and K. Ratanakhanokchai

96

Characterization and High-quality Draft Genome Sequence of Herbivorax saccincola A7, an Anaerobic,
Alkaliphilic, Hermophilic, Cellulolytic, and Xylanolytic Bacterium
S. Aikawa, S. Baramee, J. Sermsathanaswadi, P. Thianheng, C. Tachaapaikoon, A. Shikata, R. Waeonukul,
P. Pason, K. Ratanakhanokchai, and A. Kosugi

111

List of Publications: Khanok Ratanakhanokchai

112

Nakazawaea todaengensis f.a., sp. nov., a Yeast Isolated from a Peat Swamp Forest in Thailand
P. Polburee, N. Lertwattanasakul, P. Limtong, M. Groenewald, and S. Limtong

113

Meira siamensis sp. nov., a Novel Anamorphic Ustilaginomycetous Yeast Species
Isolated from the Vetiver Grass Phylloplane
S. Limtong, P. Polburee, T. Chamnanpa, P. Khunnamwong, and P. Limtong

119

List of Publications: Savitree Limtong

119

PUBLICATIONS by Former Participants in
UNESCO International Post-Graduate University Course in Microbiology (UM),UNESCO Postgraduate Inter-University Course in Biotechnology (UB),and UNESCO Training Project supported by ODA Grants for UNESCO Activities, MEXT (UO)
In Vitro Synthesis of 9,10-dihydroxyhexadecanoic Acid Using Recombinant Escherichia coli
A. Kaprakkaden, P. Srivastava, and V. S. Bisaria

120

Elicitor Enhanced Production of Protoberberine Alkaloids from In Vitro Cell Suspension Culturesof Tinospora cordifolia (Willd.) Miers ex Hook. F. & Thoms
P. Kumar, V. Srivastava, R. Chaturvedi, D. Sundar, and V. S. Bisaria

130

Organization and Characterization of Genetic Regions in Bacillus subtilis subsp. Krictiensis ATCC55079
Associated with the Biosynthesis of Iturin and Surfactin Compounds
Y. T. Kim, B. K. Park, S. E. Kim, W. J. Lee, J. S. Moon, M. S. Cho, H.-Y. Park, I. Hwang, and S. U. Kim

131

Inhibitory Effect of Obovatol from Magnolia obovata on the Salmonella Type III Secretion System
W.-S. Choi, T. H. Lee, S. J. Son, T. G. Kim, B.-M. Kwon, H.-U Son, S. U. Kim, and S.-H. Lee

149

Quality Improvement of Traditional Rice Liquor (srasor) Processing in Takeo Province, Cambodia
C. Chay, E. I. Dizon, F. B. Elegado, C. Norng, W. A. Hurtada, and L. C. Raymundo

150

ii

A Novel Probiotic Bacillus siamensis B44v Isolated from Thai Pickled Vegetables (Phak-dong)for Potential Use as a Feed Supplement in Aquaculture
R. Meidong, S. Doolgindachbaporn, W. Jamjan, K. Sakai, Y. Tashiro, Y. Okugawa, and S. Tongpim

158

Isolation and Selection of Lactic Acid Bacteria from Thai Indigenous Fermented Foods
for Use as Probiotics in Tilapia Fish Oreochromis niloticus
R. Meidong, S. Doolgindachbaporn, K. Sakai, and S. Tongpim

166

List of Publications: Saowanit Tongpim

167

Performance Validation of the Microbiologique MicrofilmTM Test System for AOAC Research Institute
Performance Tested Method SM Certification
T. L. Mai, S.-L. Sung, A. Shapovalova, H. K. Janagama, L. Vuong, A. Friedrich, D. Johnson,
L. M. Nadala, V. Nguyen, E. C. Nadala, Jr., and M. Samadpour

168

Dipstick Assay for Rapid Detection of Beer Spoilage Organisms
H. K. Janagama, T. Mai, S. Han, L. Nadala, C. Nadala, and M. Samadpour

182

Potential of Rice Stubble as a Reservoir of Bradyrhizobial Inoculum in Rice-Legume Crop Rotation
P. Piromyou, T. Greetatorn, K. Teamtisong, P. Tittabutr, N. Boonkerd, and N. Teaumroong

183

Type 3 Secretion System (T3SS) of Bradyrhizobium sp. DOA9 and Its Rolesin Legume Symbiosis and Rice Endophytic Association
P. Songwattana, R. Noisangiam, K. Teamtisong, J. Prakamhang, A. Teulet, P. Tittabutr, P. Piromyou,
N. Boonkerd, E. Giraud, and N. Teaumroong

198

List of Publications: Neung Teaumroong

199

Diversity of Endophytic Bacteria in Sweet Sorghum (Sorghum bicolor (L.) Moench)and Their Potential for Promoting Plant Growth
C. E. de Fretes, R. Suryani, Y. A. Purwestri, T. R. Nuringtyas, and D. Widianto200

α-Amylase, Glucoamylase and Isopullulanase Determine Molecular Weight of Pullulan
Produced by Aureobasidium melanogenum P16
N.-N. Liu, Z. Chi, G.-L. Liu, T.-J. Chen, H. Jiang, Z. Hu, and Z.-M. Chi

210

Cell Wall Integrity Is Required for Pullulan Biosynthesis and Glycogen Accumulationin Aureobasidium melanogenum P16
T.-J. Chen, Z. Chia, H. Jiang, G.-L. Liu, Z. Hu, and Z.-M. Chi

218

List of Publications: Zhen-Ming Chi

219

The Black Yeasts: An Update on Species Identification and Diagnosis
C. F. Cañete-Gibas and N. P. Wiederhold

221

Multicenter Evaluation of the Vitek MS v3.0 System for the Identification of Filamentous Fungi
J. Rychert, E. S. Slechta, A. P. Barker, E. Miranda, N. E. Babady, Y.-W. Tang, C. Gibas,
N. Wiederhold, D. Sutton, and K. E. Hanson

228

List of Publications: Connie F. C. Gibas

229

Coupling between D-3-phosphoglycerate Dehydrogenase and D-2-hydroxyglutarate Dehydrogenase Drives
Bacterial L-serine Synthesis
W. Zhang, M. Zhang, C. Gao, Y. Zhang, Y. Ge, S. Guo, X. Guo, Z. Zhou, Q. Liu, Y. Zhang,
C. Ma, F. Tao, and P. Xu

230

Temperature-Directed Biocatalysis for the Sustainable Production of Aromatic Aldehydes or Alcohols
J. Ni, Y.-Y. Gao, F. Tao, H.-Y. Liu, and P. Xu

239

iii

Enhancement of Gene Expression in Escherichia coli and Characterization of Highly Stable
ATP-dependent Glucokinase from Pyrobaculum calidifontis
T. Bibi, M. Ali, N. Rashid, M. A. Muhammad, and M. Akhtar

240

Preventing the N-terminal Processing of Human Interferon α-2b and Its Chimeric Derivatives
Expressed in Escherichia coli
F. Ahsan, Q. A. Gardner, N. Rashid, G. J. Towers, and M. Akhtar

251

List of Publications: Naeem Rashid

260

Engineering of an H2O2 Auto-Scavenging In Vivo Cascade for Pinoresinol Production
Y. Lv, X. Cheng, G. Du, J. Zhou, and J. Chen

262

Enhanced Extracellular Production of L-asparaginase from Bacillus subtilis 168
by B. subtilis WB600 through a Combined Strategy
Y. Feng, S. Liu, Y. Jiao, H. Gao, M. Wang, G. Du, and J. Chen

271

Chronic Exposure of μg/L Range Bisphenol A to Adult Zebrafish (Danio rerio) Leading to Adipogenesis
M. T. Ngo, T. T.-P. Doan, C. T. Nguyen, D. T.-N. Vo, C. H.-L. Do, and L. P. Nga

272

A Proteomic Analyses to Assess the Effects of Chronic Exposure of Bisphenol A
to Adult Zebrafish (Danio rerio)
N. T. Mai, D. H. L. Chi, and L. P. Nga

284

Bacillus subtilis N3 as a Biocontrol Agent for Curvularia lunata and Its Antifungal Protein Properties
R. Sowanpreecha, C. Kanchanabanca, P. Sangvanich, and P. Rerngsamran

285

Toward Non-toxic and Simple Recovery Process of Poly(3-hydroxybutyrate)
Using the Green Solvent 1,3-dioxolane
N. Yabueng and S. C. Napathorn

293

DMAPT Inhibits NF-κB Activity and Increases Sensitivity of Prostate Cancer Cells
to X-rays In Vitro and in Tumor Xenografts In Vivo
M. S. Mendonca, W. T. Turchan, M. E. Alpuche, C. N. Watson, N. C. Estabrook, H. Chin-Sinex, J. B. Shapiro,
I. E. Imasuen-Williams, G. Rangel, D. P. Gilley, N. Huda, P. A. Crooks, and R. H. Shapiro

304

Relevance of Autophagy to Fatty Liver Diseases and Potential Therapeutic Applications
S. Yan, N. Huda, B. Khambu, and X.-M. Yin

313

List of Publications: Nazmul Huda

313

Polymorphisms in MICA, but Not in DEPDC5, HCP5 or PNPLA3, Are Associatedwith Chronic Hepatitis C-related Hepatocellular Carcinoma
H. Hai, A. Tamori, L. T. T. Thuy, K. Yoshida, A. Hagihara, E. Kawamura, S. Uchida-Kobayashi,
H. Morikawa, M. Enomoto, Y. Murakami, and N. Kawada

314Long-term Follow-up of Resistance-associated Substitutions in Hepatitis C Virus in Patientsin Which Direct Acting Antiviral-based Therapy Failed
K. Yoshida, H. Hai, A. Tamori, Y. Teranishi, R. Kozuka, H. Motoyama, E. Kawamura, A. Hagihara,
S. Uchida-Kobayashi, H. Morikawa, M. Enomoto, Y. Murakami, and N. Kawada

323

List of Publications: Hoang Hai

324

Expression of Zinc Transporter Genes in Rice as Influenced by Zinc-solubilizing Enterobacter cloacae
Strain ZSB14S. Krithika and D. Balachandar

325

iv

Development of Sequence-characterized Amplified Region (SCAR) Markers as a Quality Standard of Inoculants
Based on Azospirillum
P. R. Priya, R. S. Antony, G. Gopalaswamy, and D. Balachandar
334
List of Publications: Dananjeyan Balachandar

335

Metabolomics-driven Approach to Solving a CoA Imbalance for Improved 1-Butanol Productionin Escherichia coli
T. Ohtake, S. Pontrelli, W. A. Laviña, J. C. Liao, S. P. Putri, and E. Fukusaki

336

Metabolic profiling of Garcinia mangostana (Mangosteen) Based on Ripening Stages
A. A. R. Parijadi, S. P. Putri, S. Ridwani, F. M. Dwivany, and E. Fukusaki

345

List of Publications: Sastia P. Putri

346

Inoculation with the Endophyte Piriformospora indica Significantly Affects Mechanisms
Involved in Osmotic Stress in Rice
M. A. B. Saddique, Z. Ali, A. S. Khan, I. A. Rana, and I. H. Shamsi

347

GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity
Tolerance in Transgenic Arabidopsis
Z. Xu, Q. Raza, L. Xu, X. He, Y. Huang, J. Yi, D. Zhang, H.-B. Shao, H. Ma, and Z. Ali

359

List of Publications: Zulfiqar Ali

360

Generation of a Rabbit Single-chain Fragment Variable (scFv) Antibody for Specific Detection of
Bradyrhizobium sp. DOA9 in Both Free-living and Bacteroid Forms
N. X. Vu, N. Pruksametanan, W. Srila, W. Yuttavanichakul, K. Teamtisong, N. Teaumroong,
N. Boonkerd, P. Tittabutr, and M. Yamabhai

361

Influence of Isotropic Gravity Culture on Cytoskeleton Structure and Formation of Focal Adhesionsin Human Mesenchymal Stem Cells
C. Koaykul, M. Kim, Y. Kawahara, L. Yuge, and M. Kino-oka

382Characteristics of Salted Catfish (Pangasius hypophthalmus) with Salt Concentration Variations
and Time of Fermentation
M. Karyantina, Y. W. Wulandari, and F. Kharismawati

383

Activities of International Center for Biotechnology for 2017390

Author Index

401v

ACTIVITIES OF ICBiotech390

ACTIVITIES OF INTERNATIONAL CENTER FOR
BIOTECHNOLOGY FOR FY2017
The International Center for Biotechnology (ICBiotech) was founded in April 1995 as anindependent institute in Osaka University with a mission to pursue academic advancement
and collaborative research in biotechnology. ICBiotech has its origin from the “International
Center of Cooperative Research Center in Microbial Engineering Japan (ICME)” which wasestablished in the Faculty of Engineering, Osaka University in April 1978, through renaming
to “International Center of Cooperative Research in Biotechnology (ICBiotech)” in April
1985 with the recognition of the wide acceptance and success of ICME’s activities andachievements.
ICBiotech is dedicated to promote international cooperation among Asian countries in the
aspects of research and educational advancement in the field of Biotechnology by propelling
academic interactions in Asian countries, and is committed to industrial biotechnology studies
by means of microbial engineering and related sciences, focusing on the sustainableutilization of abundant natural resources in Southeast Asian countries.
ICBiotech serves as the seat of education and research in Asia, with the support of the
Ministry of Education, Culture, Sports, Science and Technology (Monbu-kagaku-sho,
MEXT), the Japan Society for the Promotion of Science (JSPS), the Japan Science andTechnology Agency (JST), the Japan Student Service Organization (JASSO), and theauspices of the United Nations Educational, Scientific and Cultural Organization (UNESCO),
with the cooperation of Department of Biotechnology, Graduate School of Engineering,Osaka University, and researchers from prestigious universities nationwide and abroad.
In 2002 Mahidol University-Osaka University Collaborative Research Center for Bioscience and Biotechnology: MU-OU:CRC was set up at Faculty of Science, Mahidol
University as a collaborative research center to accomplish multidisciplinary research in thefield of Bioscience and Biotechnology.
The activities of the ICBiotech include:
1. Research and Education
The main area of research is industrial biotechnology rooted in microbial engineering,whilst centering on the sustainable use of agricultural and forest resources in bioresourcerich countries such as those in Southeast Asia. Research is underway in the field of cellengineering with the objectives of analyzing the cellular functions of bacteria, fungi andplants, and developing and using functions of these cells for management and rational useof biological resources that exist on our planet. ICBiotech covers three areas of Biotechnology:
1) Discovery of new functions from biological resources.
2) Bio-conversion and process engineering of biological resources.
3) Conservation of biological resources.
For under graduate and post graduate courses, ICBiotech is involved in the education
activities of Department of Biotechnology, Graduate School of Engineering as collaborating laboratories and currently covers several fields of biotechnology in education andresearch: Molecular Microbiology laboratory chaired by Prof. Takuya Nihira and Applied
Microbiology laboratory chaired by Prof. Kazuhito Fujiyama.

391

2. Participating in FrontierLab@OsakaU ’Scientific Empowerment Program for International Students’ which was created for international students to conduct thematicstudies and achieve results under the guidance of supervisors while acquiring skills
necessary for continuing research in one of Osaka University’s internationally renowned
science and technology laboratories for a period of up to 12 months.
3. Acting as collaborating laboratories with Department of Biotechnology, Graduate Schoolof Engineering, Osaka University for ‘Biotechnology Global Human Resource
Development program’, a five-year program for master’s and doctor’s degrees. The aim of
this program is to expose graduate students (privately financed as well as the Japanese
Government Scholarship students) to state-of-the-art research skills and in-depth
knowledge of advanced biology, chemistry and physics to harness the potential of
biotechnology.
4. Having been administrating and implementing the “UNESCO Biotechnology School inAsia” (2012-2016) at Department of Biotechnology, Graduate School of Engineering,Osaka University, taking over the long-termed “International Post-Graduate University
Course in Microbiology” (1972-2003) and Postgraduate Inter-University Course in
Biotechnology (2004-2007) with total of 459 alumni spreading all over Asia.
5. Serving as the secretariat for the UNESCO Regional Network for Microbiology andBiotechnology in Southeast Asia in accordance with a proposed memorandum resulting
from a Consortium Meeting of the Project “Tropical Bio-Resources and Green Chemistry
Strategy” (Osaka 2003).
6. Working as a member of the global network “Microbiological Resource Centers”(MIRCENs) operated by UNEP, UNESCO and ICRO; and as an original member of the“Regional Network for Microbiology in Southeast Asia” operated under the auspices of
UNESCO through the Fund-In-Trust of the Japanese Government (J-FIT).
7.

Promoting international cooperative researches in biotechnology with the Southeast
Asian countries related to Biotechnology, under the support of Monbu-kagaku-sho
Grant-in-Aids for International Scientific Research. In addition, ICBiotech cooperates indeveloping international organization and conducting academic seminars related tobiotechnology.

8.

Publishing Annual Reports of ICBiotech periodically.

392

Steering Committee
ChairmanProf. Kazuhito Fujiyama (Director of ICBiotech)Committee Members
Prof. Takuya Nihira (ICBiotech)
Prof. Eiichiro Fukusaki (Graduate School of Engineering)
Prof. Tsuyoshi Inoue (Graduate School of Engineering)
Prof. Hajime Watanabe (Graduate School of Engineering)
Prof. Kazumasa Hirata (Graduate School of Pharmaceutical Sciences)Prof. Masahito Taya (Graduate School of Engineering Science)Prof. Toshihiro Horii (Research Institute for Microbial Diseases)
Staff
Director/Professor
Professor
Adjunct Professor
Collaborative Professor

Dr. Kazuhito Fujiyama
Dr. Takuya Nihira
Dr. Michihiko Ike (Department of Environmental Engineering)
Dr. Watanalai Panbangred (Mahidol University, Thailand)
Dr. Ir. Irfan Dwidya Prijambada(Universitas Gadjah Mada, Indonesia)

Visiting Professor

Dr. Toshiomi Yoshida (Prof. Emer. Osaka University)
Dr. Tatsuji Seki (Prof. Emer. Osaka University)
Mr. Junji Tayama (Osaka University)
Dr. Masashi Tsuda (Kochi University)
Visiting Associate Professor Dr. Masaru Kobayashi (Kyoto University)
Visiting Research Scholar
Dr. Ingorn Kimkong (Kasetsart University, Thailand)
Dr. Nutthachai Pongprasert (KMUTT, Thailand)
Associate Professor
Dr. Shigeru Kitani
Adjunct Associate Professor Dr. Yoshinori Sumimira (Center for Global Initiatives)
LecturerDr. Ryo Misaki
Assistant Professor
Dr. Hiroshi Kinoshita
Dr. Takao Ohashi
Administrative Official
Ms. Mutsuko Tsuji
Administrative Assistant
Ms. Fumiko Tomomatsu
Ms. Chiyoko Katayama
Technical Assistant
Ms. Shizuka Kawakami
Ms. Kyoko Sugimoto393

I. COOPERATIVE RESEARCH STATION (CRS) IN SOUTHEAST ASIA
The ICBiotech, Osaka University launched out the Cooperative Research Station (CRS) in
Southeast Asia at Chalermprakiat Building, Faculty of Science, Mahidol University in 2002
through the generous support by Mahidol University. The CRS's space and equipments are
made available for Southeast Asian and Japanese researchers to undertake cooperative onsite
researches on the development of the abundant natural biological and genetic resources andtheir sustainable utilization in Southeast Asian countries through JSPS core university
program (ended in 2005), JST Special Coordination Funds for Promoting Science andTechnology (2006-2009) and JSPS Asian CORE Program (2009-2014). The CRS alsofunctioned as a lecturing and research station of the UNESCO International Post-graduate
Inter-University program, which Osaka University operated in coordination with Thai
universities. The CRS is considering support to the alumni of Osaka University and provisionof university information for recruitment of students for study in Osaka University.
Mahidol University (MU) and Osaka University (OU) together established the Mahidol
University-Osaka University Collaborative Research Center (MU-OU:CRC) for Bioscienceand Biotechnology at Faculty of Science, Mahidol University in 2002, to strengthen the
research cooperation in these fields which are amongst the most active fields of study andresearch in both universities.
Currently, MU-OU:CRC has coordinated a research projects under the jointly support ofNational Research Council of Thailand (NRCT), National Center for Genetic Engineering andBiotechnology (BIOTEC) and The Japan Society for the Promotion of Science (JSPS).
Researchers from Mahidol University, Chulalongkorn University, Kasetsart University, King
Mongkut's University of Technology Thonburi and BIOTEC participate in this project.
CRS is conducting researches on
1. Strain improvement of Bacillus thuringiensis.
2. Screening of actinomycetes for bioactive compound.
3. The soil metagenome for screening of potential polysaccharide-degrading enzymes.
4. Cell culture system for screening of bioactive compounds from Streptomycetes andenvironmental fungi.
5. Quorum sensing interference: Novel biocontrol strategies for pathogenic bacteria.

The Ceremony for the 15th Anniversary of Cooperative Research Station inSoutheast Asia
International Center for Biotechnology, Osaka University
andMahidol University and Osaka University Collaborative Research Center
for Bioscience and Biotechnology
August 4, 2017
The Sukosol Bangkok Hotel
Kamolthip 1, 2nd floor 477 Si Ayuthaya Road, Phayathai, Bangkok 10400, Thailand

394

Program
08:00 -09:00 Registration
09:00 -09:10 Report on the activity of Cooperative Research Station in Southeast Asia (OU:
CRS): Prof. Kazuhito Fujiyama, Director, ICBiotech. Osaka U
Report on the activity of Mahidol U - Osaka U Collaborative Research Center
for Bioscience and Biotechnology (MU-OU: CRC):
Prof. Watanalai Panbangred, Head, MU-OU: CRC, Faculty of Science,
Mahidol U.
09:10-09:20 Opening Address:
Assoc. Prof. Sittiwat Lertsiri, Dean, Faculty of Science, Mahidol U
09:20-09:50 Adresses:
Prof. Pornchai Matangkasombut, Emeritus Professor, Mahidol U
Prof. Toshiomi Yoshida, Emeritus Professor, Osaka U
Prof. Amaret Bhumiratana, Emeritus Professor, Mahidol U
Prof. Tatsuji Seki, Emeritus Professor, Osaka U
09:50-10:00 VDO presentation
10:00-10:15 Coffee / Tea break
10:15-10:45 Prof. Takeshi Omasa, “Next-generation therapeutic antibody production using
CHO cell platform”
10:45-11:15 Asst. Prof. Choowong Auesukaree, "Molecular mechanisms of yeast adaptive
responses to stresses associated with ethanol fermentation: from basic research
to applications"
11:15-11:45 Prof. Masahiro Kino-oka, “Cell manufacturability for regenerative medicine”
11:45-12:15 Dr. Suparerk Borwornpinyo, “An introduction to Excellent Center for Drug
Discovery (ECDD), the drug screening platform in Thailand”
12:15-12:35 Shimadzu lecture: Dr. Shin-ichi Kawano “Activitiy of Osaka UniversityShimadzu Corporation Analytical Innovation Joint Research Laboratory”
12:35-14:00 Lunch
14:00-14:30 Prof. Eiichiro Fukusaki, “Application of metabolic fingerprinting for foodevaluation”
14:30-15:00 Assoc. Prof. Manop Suphantharika, “Production and utilization of yeast extractand ß-glucan from spent brewer’s yeast”
15:00-15:15 Coffee / Tea break
15:15-15:45 Prof. Toshiya Muranaka, “Redesign of biosynthetic pathway of plant specialized
metabolites by genome editing”
15:45-16:15 Assoc. Prof. Chuenchit Boonchird, “Medicinal herbs as new source ofphytoestogens”
16:15-16:30 Closing ceremony (Professor Takuya Nihira, Professor Watanalai Panbangred,
Professor Kazuhito Fujiyama)395

II. UNESCO COURSES
1973-2003: UNESCO International Post-Graduate University Course in Microbiology
2004-2007: UNESCO Postgraduate Inter-University Course in Biotechnology
The two long-term training courses held in Japan launched in response to therecommendations concluded at a UNESCO General Conference Meeting. After theoperation of 33 batches for developing human resources in Asia, total 459 young
researchers from all over Asian countries are now playing active and leading roles incurrent biotechnology research and education in the region to contribute to thedevelopment of their own country, and consequently to Asian countries, and theestablishment of the closer and stronger network in the region.
2011-2013: UNESCO training project Supported by ODA Grants for UNESCO
Activities, MEXT (One-month lab revisit in summer)
The Program was designed to provide researchers related to the UNESCO course with theopportunities to acquire advanced skills and knowledge and to exchange ideas of research
at Japanese universities and also to create, sustain and/or strengthen collaborative linkages
with Japanese researchers. Purposes: (1) To provide the opportunity for the alumni andrelated young researchers to visit laboratories at Japanese universities to be exposed toleading-edge research, to upgrade research skills, and to facilitate exchange of researchers.
(2) To rebuild up networks among researchers between Japan and Asian countries in thefield of biotechnology. (3) To strengthen and sustain the network among the alumni of our
UNESCO courses beyond generations and countries.
Participants from 2011 to 2013
Country
Bangladesh
China
India
Indonesia
Mongolia
Pakistan
Philippines
Thailand
Vietnam
Total

2011
2
3
3
4
3
15

2012
1
1
1
1
4
1
9

2013
1
1
3
1
1
6
1
14

Total
3
2
1
6
1
1
5
14
5
38

2012-2016: UNESCO Biotechnology School in Asia (JFIT Project)
The Program was launched in October 2012. This program was an activity of the“UNESCO Extra-budgetary Program for Human Resources and Research Network in
Sciences and Technology” supported by the Japanese Fund-In-Trust (JFIT). The course
was a program of the scientific training for young Asian university-graduates in the fieldof Biotechnology and related areas resulting in achieving master degree, and theparticipants were expected to make significant contributions to an educational or scientificinstitution in the coming years in their home countries. It aimed also to strengthen the
network activities among the biotechnology scientists in the region.

396

1st Batch of school (2012 Aug - 2014 July)
Country
Cambodia
Cambodia
Laos
Laos
VietnamThailandIndonesia
Indonesia
Philippines

Affiliation
Royal U of Phnom Penh
Royal U of Phnom Penh
National U of Laos
National U of Laos
VNU, Ho-Chi Minh
Chulalongkom U
Institut Teknologi Bandung
Gadjah Mada U
UPLB

Allocated
ThailandThailandThailandIndonesia
Thailand
Japan
Japan
Japan
Japan

2nd Batch of school (2013 Aug - 2015 July)
Cambodia
Cambodia
Cambodia
Vietnam
Laos
ThailandIndonesia
Philippines

Royal U of Phnom Penh
Royal U of Phnom Penh
Royal U of Phnom Penh
VNU, Ho-Chi Minh
National U of Laos
Mahidol U
Gadjah Mada U
UPLB

ThailandThailandThailandIndonesia
Thailand
Japan
Japan
Japan

3rd Batch of school (2014 Aug - 2016 July)
Cambodia
Laos
Laos
Laos
Laos
VietnamMongolia
Indonesia
Philippines
ThailandRoyal U of Phnom Penh
National U of Laos
National U of Laos
National U of Laos
National U of Laos
VNU, Ho-Chi Minh
MUST
Institut Teknologi Bandung
UPLB
KMUTT

ThailandThailandIndonesia
ThailandThailandThailandThailand
Japan
Japan
Japan

397

U to study
KMUTT
Mahidol U
Chulalongkom U

Institut Teknologi Bandung

Kasetsert U
Osaka U
Osaka U
Osaka U
Osaka U

KMUTT
Mahidol U
Chulalongkom U

Number

2
2
1
1
1
1
1
1
1

Kasetsert U
Osaka U
Osaka U
Osaka U

2
2
1
1
1
1
1
2

KMUTT
Chulalongkom U
ITB
KMUT
Chulalongkorn U
KMUTT
Mahidol U
Osaka U
Osaka U
Osaka U

2
1
1
1
1
1
1
1
1
1

Institut Teknologi BandungIII. WORKSHOPThe 7th Workshop on Collaboration
between Osaka University and Japanese companies

for global human resource development in the field of biotechnology

January 19, 2018
International Center for Biotechnology, Osaka University
Sponsorship; International Center for Biotechnology, Osaka University
Co-sponsored; International Research Initiative for Frontier of Industrial Biotechnology, Osaka University

The seventh Workshop on Collaboration between Osaka University and Japanesecompanies was held. Representatives of industries engaged in the biotechnology industry
were introduced to the international students enrolled at Osaka University for the purpose of
attaining international human resource development. A total of eight enthusiastic companies
attended the meeting. Companies, faculty members and students introduced themselves andtheir activities to one another. Students were able to know about the Japanese companies andhow to apply their scholarly works in the industrial biotechnology setting. Adding to the great
success of this meeting are the lively discussions and exchange meetings which have resulted
in a deepened understanding of each other.
14:00: Opening remark by Prof. Kazuhito Fujiyama
14:00-14:45: Special lecture “Company Introduction and its HR Strategy”
by Yoshiro Numata (Nagase & Co)
14:45-15:05: Coffee Break
15:15-17:40: Group talking with individual company(45 min each, three rounds at α building and β building in ICBiotech)
15:15-16:00: 1st Round
16:05-16:50: 2nd Round
16:55-17:40: 3rd Round
Attending companies are:
Ajinomoto General Foods, Inc. (α-401), Kao (α-502)
Lion Corp. (α-401), Nagase & Co. (α-504)
NS Solutions (α-504), Rohto Pharmaceuticals (α-506)
Shimadzu Corp. (β-303), Sysmex Corp. (α-501)
17:45-19:30: Simple party with light meal at International saloon (β-102).

398

IV. JASSO STUDENT EXCHANGE SUPPORT PROGRAM
Scholarship for Short Stay/ Short Visit Program (SSSV)This is a field study program jointly operated with GLOCOL (Global Collaboration
Center), Osaka University and several universities in Thailand. In 2017, 25 first year master's
degree students of Osaka University visited 4 universities in Thailand between August 6 and
September 14, and 24 postgraduate students from Thailand visited Osaka University between
October 2017 and March 2018, which enhanced mutual interactions.

V. JST Japan-Asia Youth Exchange Program in Science(SAKURA Exchange Program in Science)
Purpose of the Program: Promoting science and technology is a key engine to materializea bright future of Asia and it is vitally important to enhance the exchange of youths in Asian
countries and Japan who will play a crucial role in the field of science and technology. Basedon this concept, “Japan-Asia Youth Exchange Program in Science” (SAKURA Exchange
Program in Science) is the program for enhancing exchanges between Asia and Japan of the
youths who will play a crucial role in the future field of science and technology through theclose collaboration of industry-academia-government by facilitating short-term visits ofcompetent Asian youths to Japan. This program aims at raising the interest of Asian youths
toward the leading Japanese science and technologies at Japanese universities, research
institutions and private companies.
Schedule
November 5
November 6
November 7

November 8
November 9
November 10
November 11
November 13
November 14

Arriving
Orientation, Lab visit, Cutting-edge biotechnology experiment:
briefing and experiment
Seminar on advanced biotechnology research
Cutting-edge biotechnology experiment: data analysis and discussion
Guidance of English course
Lecture of cutting-edge biotechnology: Prof. Yamasaki, Osaka Prefectural University
Lab Visit in RIMD, Institute for Protein Research, OU and Hitz
Visit to Kiku-masamune Sake Brewery Museum, Himeji Castle,
and Kikkoman Takasago Factory
Visit to Shimadzu Company in Kyoto and Kinkakuji-Temple
Visit to National Museum of Ethnology and Museum
Presentation of task, Certificate award
Closing Ceremony
Back home

VI. SCIENTIST EXCHANGES
Record of Scientist Exchange (FY2017)

From Japan to counterpart countries / Between counterpart countries*Please refer to the office.

399

VII. GUESTS/VISITORS*Please refer to the office.
VIII. COMMITTEE MEETING 2017
Steering Committee Meetings of ICBiotech were convened as follows:・September 26, 2017: Discussion on inviting Visiting Research Scholars:
Mr. Ronilo Jose Danila (October 1, 2017-September 30, 2017)
Mr. Surya Agung Priyambada (October 1, 2017-September 30, 2017)
・October 11, 2017: Discussion on inviting a Visiting Research Scholar:
Ms. Dian Anggraini Suroto (October 17, 2017- September 30, 2017)
・November 29, 2017: Discussion on the employment of the full-time researcher:
Dr. Ingorn Kimkong (February 16, 2018 - March 15, 2018)
Dr. Nutthachai Pongprasert (February 16, 2018 - March 15, 2018)
Nominating Prof. Takuya Nihira for Professor Emeritus
・December 19, 2017: Concluding the cross appointment agreement:
Dr. Vu Thu Trang (March 1, 2018 - August 31, 2018)
・February 27, 2018
Report on:
1. JST Sakura Exchange Program in Science
2. 7th Workshops (January 19, 2018) on Collaboration between Osaka University
and Japanese Companies
3. JASSO Short-stay and Short-visit program 2017
(Aug. 5, 2017 - Sept. 14, 2017 sending Japanese students to Thailand,
Oct. 1, 2017 to March 31, 2018 accepting Thai students)
4. Concluding MOU*August 3, 2017: Faculty of Tropical Medicine, Mahidol University, Thailand*February 5, 2018: School of Biotechnology and Food Technology, Hanoi
University of Science and Technology, Vietnam
5. Concluding the cross appointment agreement
6. Two Visiting Research Scholars
Dr. Ingorn Kimkong from Thailand, February 16 - March 15, 2018
Dr. Nutthachai Pongprasert from Thailand, February 16 - March 15, 2018

7. Adjunct Professors for the FY2018
8. Evaluation Committee Meeting (February 27, 2018)
Discussion on:
1. Revising the regulations of ICBiotech
2. Conferring the title of Visiting Professors from abroad for FY2018
3. Employment of a researcher by the cross appointment agreement
4. Inviting Visiting Professors from Japanese universities for FY2018
5. Inviting a visiting research scholars
6. Inviting a part-time lecturer
7. Selection of members of the Evaluation Committee Meeting400