091_12_AKR Asthma Poster03 840x552mm:Layout 1 02/05/2012 12:33 Page 1

Inflammatory Mechanisms of Asthma
Clonality

Applications

HostSpecies
reactivity

Productcode

ADAM33 - Cytoplasmic domain

P

ICC/IF, IHC-R, WB

RbHu

ab39193

B7H4P

ELISA, WB

Goat

Hu

ab77281Cathepsin S - Catalytic domain

PWB

Rb

Hu

ab50400

CCR3

P

ICC, IHC-P, WB

Goat

Hu

ab1667CCR4P

ELISA, Flow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, WB

Goat

Hu, CynMk

ab1669CCR6P

IHC-P, WB

Rb

Hu, Ms, Rat

ab78429CCR8P

IHC-FoFr, IHC-Fr, IHC-P, WB

Goat

Ms

ab1663

CD14 [61D3]

M

Flow Cyt, IHC-Fr, IP, Neut

Ms

Huab25625

CD252 [MRC OX89]

M

Flow Cyt, IHC-Fr

Rat

Ms

ab7434

CD28 [15E8] (FITC)M

Flow Cyt, IP, WB

Ms

Huab42742

CD34 [MEC 14.7] Hematopoietic Stem Cell Marker

M

ELISA, Flow Cyt, ICC/IF, IF, IHC-FoFr, IHC-Fr,IHC-P, IP, WB

Rat

Ms

ab8158

CD40 [EBI-19-21]

M

ELISA, Flow Cyt, FuncS

RbHu

ab79696

CD80 [EP1155Y]

M

Flow Cyt, ICC, IHC-P, WB

Rb

Hu, Rat

ab53003

CD86 [EP1158Y]

M

Flow Cyt, ICC, IHC-Fr, IHC-P, IP, WB

Rb

Hu, Rat

ab53004

CXCR2PICC/IF, IHC-Fr, IHC-P, IP, WB

Rb

Hu, Ms, Rat

ab14935

EotaxinP

ICC/IF, IHC-R, WB

RbHu

ab8018

Eotaxin 2

P

ELISA, Neut, sELISA, WB

Goat

Ms

ab84016

Eotaxin 3

PWB

Ms

Hu

ab67260

Fc epsilon RI

M

WB

Ms

Hu

ab54660

HistamineP

ICC/IF, IHC-FoFr

RbRat

ab43870

HLA DMBM

WBMs

RecFragab55150

HLA DRB1

PWB

Ms

Huab89315

ICOS

P

ELISA, IHC-P, WB

Goat

Hu

ab111247

IL3 [4E2]M

ELISA, I-ELISA, WB

Ms

Huab9815IL4P

ELISA, IHC-P, Neut, sELISA, WB

RbHu

ab9622IL4 [BVD4-1D11]M

ELISA, Flow Cyt, IHC-Fr, WB

Rat

Ms

ab11524

IL4R (phospho Y497)PELISA, ICC/IF, WB

RbHu

ab61099

IL5 [JES1-39D10] (Biotin)

M

ELISA, Flow Cyt, IF, WB

Rat

Hu

ab24952

IL8 [EP117Y]

M

WB

RbHu

ab52612IL9P

ELISA, WB

RbHu

ab76229IL13P

ELISA, Neut, WBRb

Huab9576

Interferon gamma

P

ELISA, IHC-P, Neut, WB

RbHu

ab9657IP10P

ELISA, Neut, WB

Rb

Ms

ab9938Macrophage Inflammatory
Protein 3 beta - C-terminalP

ELISA, IHC-P

Rb

Hu

ab80145

MCP3 [1015]

M

ELISA, WB

Ms

Hu, Ratab18694

MCP4M

ELISA, WB

Ms

Hu

ab88080

MDC - Aminoterminal end

P

IHC-P, WB

Rb

Ms

ab53002

RANTESPELISA, IHC-P, Neut, WB

RbHu

ab9679SDF1 [MM0211-9N26]

M

Flow Cyt, IHC-P

Ms

Hu

ab89321

TARC (Biotin)P

ELISA, sELISA, WBRbHu

ab84279

AlveoliBronchial
tube

Contracted muscle

Risks:• Environmental allergens- Pollens- Dust Mites- Animal hair/dander- Molds• Hay fever and other allergies• Eczema• Smoke• Pollutants• Chemicals• Upper respiratory infection

MuscleNormalConstrictedairway• Physical exertion/exercise• Weather changes

Intrinsic

TargetTriggers:

Extrinsic

Antibodies:• Genetic predisposition*- Chr. 5 - IL4, IL13,CD14, ADRB2- Chr. 6 - HLA, TNF- Chr. 11 - FCER1B- Chr. 16 - IL4RA- Chr. 20 - ADAM33*Genes for which positive association has
been replicated more than 10 times.

Mucus

Asthmatic

Open airway

Inflammation
Mucus
IL4Ra

Allergens

IL13
IL4

TH2 cellTargetReacts Assaywith
type

Featured products:
Sampletype

Sensitivity

Generalrange

Number Productof plates
code

Clonality

Applications

Host

ICC/IF, IHC-Fr, IHC-P, IP, WB

Ms

CD14Husandwich ELISA

Cell culture supernatant, plasma, serum, urine< 6 pg/ml

6000 - 8.23 pg/ml

1ab99989

EotaxinMs

sandwich ELISA

Cell culture supernatant, plasma, serum< 1 pg/ml

100 - 1.02 pg/ml1ab100680

Eotaxin 2

Hu

sandwich ELISA

Cell culture supernatant, plasma, serum, urine< 2 pg/ml

1000 - 1.37 pg/ml

1ab100509

IL3

Husandwich ELISA

Cell culture supernatant, plasma, serum, urine< 20 pg/ml

12000 - 16.46 pg/ml

1ab100569

IL4

Husandwich ELISA

Cell culture supernatant, plasma, serum< 0.5 pg/ml

35 - 1.1 pg/ml

1ab46058

MMP9 antibody [5G3] (ab119906)

IL4R

Hu

sandwich ELISA

Cell culture supernatant, plasma, serum< 11.5 pg/ml1000 - 31.25 pg/ml

1ab46022

ClonalityIL5Hu

sandwich ELISA

Cell culture supernatant, plasma, serum< 5 pg/ml

250 - 7.8 pg/ml

1ab46026

IL8Hu

sandwich ELISA

Cell culture supernatant, plasma, serum< 25 pg/ml

2000 - 62.5 pg/ml

1ab46032

IL9Hu

sandwich ELISA

Cell culture supernatant, plasma, serum, urine< 0.1 ng/ml

30 - 0.04 ng/ml

1ab100577

IL13

Husandwich ELISA

Cell culture supernatant, plasma, serum< 1.5 pg/ml

100 - 3.12 pg/ml

1ab46038

Interferon gamma Hu

sandwich ELISA

Cell culture supernatant, plasma, serum< 0.69 pg/ml

25 - 0.78 pg/ml

1ab46048

IP10

sandwich ELISA

Cell culture supernatant, plasma, serum< 5.7 pg/ml

200 - 6.25 pg/ml

1ab83700

Hu

M

M

Hu

sandwich ELISA

Cell culture supernatant, plasma, serum, urine< 2 pg/ml

500 - 2.05 pg/ml

1ab100601

MCP3

Hu

sandwich ELISA

Cell culture supernatant, plasma, serum, urine< 20 pg/ml

6000 - 24.58 pg/ml

1ab100588

MCP4

Hu

sandwich ELISA

Cell culture supernatant, plasma, serum, urine< 3 pg/ml

200 - 3.13 pg/ml

1ab100589

RANTESHu

sandwich ELISA

Cell culture supernatant, plasma, serum, urine< 3 pg/ml

2000 - 2.74 pg/ml

1ab100633

TARC

Hu

sandwich ELISA

Cell culture supernatant, plasma, serum, urine< 5 pg/ml

1000 - 4.1 pg/ml

1ab100644

TNF alphaHusandwich ELISA

Cell culture supernatant, plasma, serum< 10 pg/ml

800 - 25 pg/ml

1ab46087

Applications

Host

Flow Cyt, ICC/IF, IHC-P, WB

Ms

Species crossreactivity
Hu

Eosinophil

Species crossreactivity
Hu, MsMucus
overproduction
Airway hyperresponsivenessIL10 antibody (ab34843)
Clonality
P

Macrophage
Inflammatory
Protein 3 beta

IL5

E Cadherin antibody [HECD-1] (ab1416)

Applications

Host

Species crossreactivity

ELISA, ICC/IF, IHC-Fr, IHC-P,
IP, Neut, RIA, WB

RbHu

HostSpecies crossreactivity
Hu, Ms, Chk,Dog, Gpig, Pig

IL9Histamine
Proteases
Cytokines
Leukotrienes
Prostaglandins

IL4

IL13

Dendriticcell

Mast cell
B Cell

TNF alpha antibody (ab6671)
Clonality
P

Applications

ELISA, ICC/IF, IHC-FoFr, IHC-Fr, Rb
IHC-P, Neut, WB

091_12_AKR

ELISA kits:

IgE
Airway smooth muscle

Discover more at abcam.com/Asthma
Copyright © 2012 Abcam, All Rights Reserved. The Abcam logo is a registered trademark.
All information / detail is correct at time of going to print.

IHC images of normal and asthmatic airways courtesy of Professor Stephen T Holgate, University of Southampton, UK.