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accordingly. Category:Terms of use Category:Domain namesHuman leukocyte antigens and leukaemia: what can we learn froma genetic perspective? This review describes approaches to the study of gene expression in leukaemia and describes some of thegenetic changes occurring in the disease. Two aspects of this field are addressed: alterations in genes associated with apredisposition to the disease and the expression of genes involved in normal growth control. The finding that the most common
genetic change in leukaemia is a translocation between the immunoglobulin heavy chain and myeloma oncogene suggests thatthese two genes, when juxtaposed, may be directly responsible for malignancy. This has yet to be confirmed. Another common
genetic event in acute leukaemia is that chromosome 8q24 is gained as a result of a gene on this chromosome being expressed.
The fact that the p53 tumour suppressor gene is located on chromosome 17p and is mutated in up to half of all haematologicalmalignancies may be relevant to our understanding of the pathogenesis of these diseases. Analysis of gene expression in acuteleukaemia using an expression microarray approach has led to the identification of a number of genes with abnormal expression
in the disease that may be important in leukaemia cell growth and differentiation. A 'first pass' search in the literature wasperformed to identify all genes reported to be altered in leukaemia cell lines or primary cells. A core list of 19 genes wasextracted from this review and an additional 16 genes identified from the literature searches were included. This set of genes
was used as a basis to determine whether they could be clustered into groups based on their pathway involvement in leukaemia.
Our analysis of the data yielded three main categories: 1. genes with altered expression in common between cell lines andleukaemic cells but also present in normal tissues; 2. genes with altered expression in leukaemia cell lines but absent in normaltissues; and 3. genes with altered expression in leukaemic cells and absent in cell lines. Overall, this is an under-investigatedfield at present. With regard to the first group of genes, as the alteration in cell lines is often accompanied by leukaemia, someevidence for a link with malignancy is expected. With the second category, genes such as topois f678ea9f9e
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