ANNUAL REPORTS
OF
INTERNATIONAL CENTER FOR BIOTECHNOLOGYOSAKA UNIVERSITY

VOL. 33, 2011

DIRECTOR/EDITORTAKUYA NIHIRAASSISTANT EDITOR

KAZUHITO FUJIYAMASHIGERU KITANIHIROSHI KINOSHITARYO MISAKITAKAO OHASHISECRETARY

RINA TADAFUMIKO TOMOMATSUYUKIKO SETOCHUNXIANG WANG

The Annual Report is published to record the activity of theInternational Center for Biotechnology (ICBiotech) and issued once in eachfiscal year. It contains scientific articles, progress reports, letters, andannouncement from the Center. This volume includes publications by theformer participants in UNESCO courses. The editor welcomes thesubmission of appropriate articles from all persons who are concerned withthe activity of the Center. All the contributions, however, will be
reviewed by editors before their acceptance. The scientific paper hereinshould be treated as personal communications and not treated as original
publications. The Annual Report is distributed upon request to theInternational Center for Biotechnology, Osaka University, 2-1 Yamadaoka,
Suita, Osaka 565-0871, Japan (e-mail: info@icb.osaka-u.ac.jp). The
editors are very grateful to Ms. Fumiko Sawazumi for her technical help.

CONTENTS
Avenolide, a Streptomyces Hormone Controlling Antibiotic Production
in Streptomyces avermitilis
S. Kitani, K. T. Miyamoto, S. Takamatsu, E. Herawati, H. Iguchi,
K. Nishitomi, M. Uchida, T. Nagamitsu, S. Omura, H. Ikeda, andT. Nihira

1

VisG is Essential for Biosynthesis of Virginiamycin S, a StreptograminType B Antibiotic, as a Provider of the Nonproteinogenic Amino Acid
Phenylglycine
F. Ningsih, S. Kitani, E. Fukushima, and T. Nihira

7

The Autoregulator Receptor Homologue AvaR3 Plays a Regulatory
Role in Antibiotic Production, Mycelial Aggregation and Colony
Development of Streptomyces avermitilis
K. T. Miyamoto, S. Kitani, M. Komatsu, H. Ikeda, and T. Nihira

15

Identification and Characterization of Penicillium citrinum VeA and
LaeA as Global Regulators for ML-236B Production
S. Baba, H. Kinoshita, and T. Nihira

25

Heterologous Expression System in Aspergillus oryzae for Fungal
Biosynthetic Gene Clusters of Secondary Metabolites
K. Sakai, H. Kinoshita, and T. Nihira

26

Total Synthesis and Absolute Configuration of Avenolide, Extracellular
Factor in Streptomyces avermitilis
M. Uchida, S. Takamatsu, S. Arima, K.T. Miyamoto, S. Kitani, T. Nihira,H. Ikeda, and T. Nagamitsu

27

Identification of Actinomycetes from Plant Rhizospheric Soils with Inhibitory
Activitity against Colletotrichum spp., the Causative Agent of Anthracnose
DiseaseB. Intra, I. Mungsuntisuk, T. Nihira, Y. Igarashi, and W. Panbangred

28

Stable Coexpression of Two Human Sialylation Enzymes in Plant
Suspension-cultured Tobacco Cells
H. Kajiura, R. Misaki, K. Fujiyama, and T. Seki

29

Jack Bean -Mannosidase Digestion Profile of Hybrid-type Nglycans: Effect of Reaction pH on Substrate Preference
K. Dohi, J. Isoyama-Tanaka, R. Misaki, and K. Fujiyama

36

Arabidopsis 1,2-xylosyltansferase: Substrate Specificity and Participationin the Plant-specific N-glycosylation PathwayiH. Kajiura, T. Okamoto, R. Misaki, Y. Matsuura, and K. Fujiyama

42

Improved Expression and Characterization of Recombinant Human
Golgi 1,2-mannosidase I Isoforms (IA2 and IC) by Escherichia coli
J. Isoyama-Tanaka, K. Dohi, R. Misaki, and K. Fujiyama

49

Reduced Immunogenicity of Arabidopsis hgl1 Mutant N-glycans
Caused by Altered Accessibility of Xylose and Core Fucose Epitopes
H. Kaulfürst-Soboll, S. Rips, H. Koiwa, H. Kajiura, K. Fujiyama,
and A. von Schaewen

50

Double-knockout of Putative Endo--N-acetylglucosaminidase (ENGase)
Genes in Arabidopsis thaliana: Loss of ENGase Activity Induced
Accumulation of High-mannose Type Free N-glycans Bearing N,N’acetylchitobiosyl Unit
Y. Kimura, Y. Takeoka, M. Inoue, M. Maeda, and K. Fujiyama

51

N-terminal Vacuolar Sorting Signal at the Mouse Antibody Alters
the N-linked Glycosylation Pattern in Suspension-cultured Tobacco
BY2 Cells
R. Misaki, Y. Sakai, T. Omasa, K. Fujiyama, and T. Seki

52

Enhancement of Sialylation on Humanized IgG-like Bispecific
Antibody by Overexpression of 2,6-sialytransferase Derived from
Chinese Hamster Ovary Cells
M. Onitsuka, W. D. Kim, H. Ozaki, A. Kawaguchi, K. Honda, H. Kajiura,
K. Fujiyama, R. Asano, I. Kumagai, H. Ohtake, and T. Omasa

53

Superior Thermotolerance of Saccharomyces cerevisiae for Efficient
Bioethanol Fermentation Can Be Achieved by Overexpression of RSP5
Ubiquitin Ligase
H. Shahsavarani, M. Sugiyama, Y. Kaneko, C. Boonchird, and S. Harashima

54

Highly Efficient Bioethanol Production by Sacccharomyces cerevisiae
Strain with Multiple Stress Tolerance to High Temperature, Acid and Ethanol
S. Benjaphokee, D. Hasegawa, D. Yokota, T. Asvatak, C. Auesukaree,
M. Sugiyama, Y. Kaneko, C. Boonchird, and S. Harashima

66

List of Publication: Satoshi Harashima

67

Bioproduction of Vanillin Using an Organic Solvent-tolerant Brevibacillus
agri 13
N. Wangrangsimagul, K. Klinsakul, A. S. Vangnai, J. Wongkongkatep,
P. Inprakhon, K. Honda, H. Ohtake, J. Kato, and T. Pongtharangkul

68

Bacteria Interface Pickering Emulsions Stabilized by Self-assembledii

Bacteria – Chitosan Network
P. Wongkongkatep, K. Manopwisedjaroen, P. Tiposoth, S. Archakunakorn,
T. Pongtharangkul, M. Suphantharika, K. Honda, I. Hamachi, andJ. Wongkongkatep

77

List of Publication: Hisao Ohtake

78

Use of Ultra-performance Liquid Chromatography/time-of-flight Mass
Spectrometry with Nozzle-skimmer Fragmentation for Comprehensive
Quantitative Analysis of Secondary Metabolites in Arabidopsis thaliana
T. Sugimoto, T. Bamba, Y. Izumi, H. Nomura, T. Shiina, and E. Fukusaki

79

Predication of Japanese Green Tea (Sen-cha) Ranking by Volatile
Profiling Using Gas Chromatography Mass Spectrometry and Multivariate Analysis
K. Jumtee, H. Komura, T. Bamba, and E. Fukusaki

89

List of Publication: Eiichi Fukusaki

90

Purification and Characterization of Phytase from Klebsiella pneumoniae
9-3B
L. Escobin-Mopera, M. Ohtani, S. Sekiguchi, T. Sone, A. Abe, M. Taanaka,
V. Meevootisom, and K. Asano

91

Characterization of Inago1 and Inago2 Retrotransposons in MagnaportheoryzaeE. Sanchez Jr., K. Asano, and T. Sone

97

List of Publication: K. Asano

98

Production and Accumulation of 4-O-Methyl--D-Glucuronosyl-xylotriose by
Growing Culture of Thermophilic Anoxybacillus sp. Strain JT-12
J. Thitikorn-amorn, K. L. Kyu, K. Sakka, and K. Ratanakhanokchai

99

Characterization of Paenibacillus curdlanolyticus B-6 Xyn 10D, a Xylanase
Thai Contains a Family 3 Carbohydrate-binding Module
M. Sakka, Y. Higashi, T. Kimura, K. Ratanakhanokchai, and K. Sakka

109

Maklamicin, an Antibacterial Polyketide from an Endophytic
Micromonospora sp.
Y. Igarashi, H. Ogura, K. Furihata, N. Oku, C. Indananda, and
A. Thamchaipenet

110

Prajinamide, a New Modified Peptide from a Soil-derived Streptomyces
Y. Igarashi, Y. Tanaka, M. Ikeda, T. Oikawa, S. Kitani, T. Nihira,P. Mongkol, M. Janhom, and W. Panbangred

115

iii

Identification and Characterization of Leucocyclicin Q, a Novel Cyclic
Bacteriocin Produced by Leuconostoc mesenteroides TK41401
Y. Masuda, H. Ono, H. Kitagawa, H. Ito, F. Mu, N. Sawa, T. Zendo,
and K. Sonomoto

118

Lantibiotic Transporter Requires Cooperative Functioning of thePeptidase Domain and the ATP Binding Domain
M. Nishie, M. Sasaki, J. Nagao, T. Zendo, J. Nakayama, andK. Sonomoto

125

List of Publication: Kenji Sonomoto

126

Acceleration of Nonylphenol and 4-tert-octylphenol Degradation in
Sediment by Phragmites australis and Associated Rhizosphere Bacteria
T. Toyama, M. Murashita, K. Kobayashi, S. Kikuchi, K. Sei,
Y. Tanaka, M. Ike, and K. Mori

128

Molecular Cloning and Characterization of the srdBCA Operon,
Encoding the Respiratory Selenate Reductase Complex, from theSelenate-reducing Bacterium Bacillus selenatarsenatis SF-1
M. Kuroda, N, Yamashita, E. Miwa, K. Imao, N. Fujimoto, H. Ono,
K. Nagano, K. Sei, and M. Ike

135

List of Publication: Michihiko Ike

136

Genomic Organization of -Guaiene Synthase Genes in Aquilaria crassna
and Its Possible Use for the Identification of Aquilaria Species
Y. Kumeta and M. Ito137

Regular Ingestion of Cinnamomi Cortex Pulveratus Offers Gastroprotective
Activity in MiceJ. M. Tankam, Y. Sawada, and M. Ito

143

List of Publication: Michiho Ito

144

Ogataea phyllophila sp. nov., Candida chumphonensis sp. nov. andCandida mattranensis sp. nov., Three Methylotrophic Yeast Species
from Phylloplane in Thailand
N. Koowadjanakul, S. Jindamorakot, W. Yongmanitchai, and
S. Limtong

145

Candida saraburiensis sp. nov. and Candida prachuapensis sp. nov.,
Xylose-utilizing Yeast Species Isolated in Thailand
S. Nitiyon, C. Boonmak, S. Am-In, S. Jindamorakot, H. Kawasaki,
W. Yongmanitchai, and S. Limtong

156

iv

List of Publication: Savitree Limtong

157

Culture-independent Phylogenetic Analysis of the Microbial
Community in Industrial Sugarcane Bagasse Feedstock Piles
U. Rattanachomsri, P. Kanokratana, L. Eurwilaichitr, Y. Igarashi,
and V. Champreda

158

Biocatalytic Esterification of Palm Oil Fatty Acids for Biodiesel
Production Using Glycine-based Cross-linked Protein CoatedMicrocrystalline Lipase
M. Raita, T. Laothanachareon, V. Champreda, and N. Laosiripojana

166

List of Publication: Verawat Champreda

167

Bioproduction of Vanillin Using an Organic Solvent-tolerant
Brevibacillus agri 13
N. Wangrangsimagul, K. Klinsakul, A. S. Vangnai,
J. Wongkongkatep, P. Inprakhon, K. Honda, H. Ohtake, J. Kato,and T. Pongtharangkul

168

Garvieacin Q, a Novel Class II Bacteriocin from Lactococcus
garvieae BCC 43578
A. Tosukhowong, T. Zendo, W. Visessanguan, S. Roytrakul,
L. Pumpuang, J. Jaresitthikunchai, and K. Sonomoto

177

Dynamic Microbial Communities in an Earthen Shrimp Pond
during the Shrimp Growing Period
S. Sombatjinda, N. Boonapatcharoen, M. Ruengjitchatchawalya,
C. Wantawin, B. Withyachumnarnkul, and S. Techkarnjanaruk

182

List of Publication: Marasri Ruengjitchatchawalya

192

List of Publication: Kanokwan Poomputsa

193

PUBLICATIONS by Former Participants in
UNESCO International Post-Graduate University Course in Microbiology (UM),UNESCO Postgraduate Inter-University Course in Biotechnology (UB),and UNESCO Training Project supported by ODA Grants for
UNESCO Activities, MEXT (UO)
A Case Study of the Indigenous Technology for Making WhiteSoft Cheese Kesong puti in Lumban, Laguna, Philippines
E. M. Aquino, N. E. Tapay, and V. L. Barraquiov

194Meta-analysis for Effect of Dietary Isoflavones on Breast
Density and Hot Flush Suppression
So-Jeen Kwon and Bang-Ho Song

206

Identification of Actinomycetes from Plant Rhizospheric
Soils with Inhibitory Activity against Colletotrichum spp., theCausative Agent of Anthracnose Disease
B. Intra, I. Mungsuntisuk, T. Nihira, Y. Igarashi, and W. Panbangred

207

Lipase-catalyzed Synthesis of Hydrophobically Modified
Dextrans: Activity and Regioselectivity of Lipase from
Candida rugosa
K. Kaewprapan, J. Wongkongkatep, W. Panbangred,
P. Phinyocheep, E. Marie, A. Durand, and P. Inprakhon

216

The Bioplastic, Poly(3-hydroxybutyrate) Production by
Batch Cultivation of Bacillus megaterium P-12 from
Sugarcane Liquor with Different C/N Ratio
W. Muangkao, N. Thongchul, and S. Kulpreecha

217

Bioconversion of Paper Sludge to Biofuel by Simultaneous
Saccharification and Fermentation Using a Cellulase ofPaper Sludge Origin and Thermotolerant Saccharomyces
cerevisiae TJ14
J. Prasetyo, K. Naruse, T. Kato, C. Boonchird, S. Harashima,
and E. Y. Park

221

Genome-wide Survey Reveals Dynamic Widespread Tissuespecific Changes in DNA Methylation during Development
P. Liang, F. Song, S. Ghosh, E. Morien, M. Qin, S. Mahmood,
K. Fujiwara, J. Igarashi, H. Nagase, and W. A. Held234

The Development of a Novel Serotyping-NS1-ELISA to
Identify Serotypes of Dengue Virus
C. Puttikhunt, T. Prommool, N. U-thainual, P. Ong-ajchaowlerd,
K. Yoosook, C. Tawilert, T. Duangchinda, A. Jairangsri,
N. Tangthawornchaikul, P. Malasit, and W. Kasinrerk

250

Biotechnological Potential of Inulin for Bioprocesses
Zhen-Ming Chi, Tong Zhang, Tian-Shu Cao, Xiao-Yan Liu,
Wei Cui, and Chun-Hai Zhao

256

Mig 1 is Involved in Mycelial Formation and Expression of
the Genes Encoding Extracellular Enzymes in Saccharomycopsis
fibuligera A11
Guang-Lei Liu, Dong-Sheng Wang, Ling-Fei Wang,vi

Shou-Feng Zhao, and Zhen-Ming Chi

265

List of Publication: Zhenming Chi

266

Efficient Saccharification of Ammonia Soaked Rice Straw
by Combination of Clostridium thermocellum Cellulosome
and Thermoanaerobacter brockii -Glucosidase
R. Waeonukul, A. Kosugi, C. Tachaapaikoon, P. Pason,
K. Ratanakhanokchai, P. Prawitwong, L. Deng, M. Saito, and Y. Mori

267

Isolation and Characterization of a New Cellulosomeproducing Clostridium thermocellum Strain
D. Tachaapaikoon, A. Kosugi, P. Pason, R. Waeonukul,
K. Ratanakhanokchai, K. L. Kyu, T. Arai, Y. Murata, and Y. Mori

273

Highly Regioselective Glucosylation of 2’-Deoxynucleosides
by Using the Crude -glycosidase from Bovine LiverMin Ye, Chun-Yang Yu, Ning Li, and Min-Hua Zong

274

Optimization of Culture Conditions to Produce High Yieldsof Active Acetobacter sp. CCTCC M209061 Cells for AntiPrelog Reduction of Prochiral Ketones
Xiao-Hong Chen, Wen-Yong Lou, Min-Hua Zong, and T. J. Smith

280

List of Publication: Min-Hua Zong

281

Invasive Myceliophthora thermophila Infection Mimicking
Invasive Aspergillosis in a Neutropenic Patient: A New
Cause of Cross-reactivity with the Aspergillus Galactomannan
Serum Antigen Assay
E. Morio, F. Fraissinet, T. Gastinne, P. L. Pape, J. Dellaunay,
L. Sigler, C. F. C. Gibas, and M. Miegeville282

Screening of Some Enzymes and Nutrients in Radish(Raphanus sativus L.) Root
M. G.. S. Jahan, M. S. Pervin, M. B. Uddin, N. K. Sana,and M. H. Rahman

286

In Vivo Regulation of Alcohol Dehydrogenase and Lactate
Dehydrogenase in Rhizopus oryzae to Improve L-lactic Acid
Fermentation
S. Thitiprasert, S. Sooksai, and N. Thongchul

291

One-pot Bio-synthesis: N-acetyl-D-neuraminic Acid Production
by a Powerful Engineered Whole-cell Catalyst
Fei Tao, Yinan Zhang, Cuiqing Ma, and Ping Xu

309

vii

List of Publication: Ping Xu

316

Family B DNA Polymerase from a Hyperthermophilic Archaeon
Pyrobaculum calidifontis: Cloning, Characterization and PCR
Application
S. F. Ali, N. Rashid, T. Imanaka, and M. Akhtar

319

Gene Cloning and Characterization of TK 1392, an NADH Oxidasefrom Thermococcus kodakaraensis with a Distinct C-terminal Domain
N. Rashid, S. Akmal, and M. Akhtar

325

List of Publication: Naeem Rashid

326

Purification and Characterization of Methyl Parathion Hydrolasefrom Burkholderia cepacia Capable of Degrading Organophosphate
Insecticides
A. Ekkhunnatham, B. Jongsareejit, W. Yamkunthong, and
J. Wichitwechkarn

327

Antibiotic Contaminants in Coastal Wetlands from Vietnamese
Shrimp Farming
Hoang Thi Thanh Thuy, Le Phi Nga, and Tu Thi Cam Loan

335

Inhibition of NF-B and DNA Double-strand Break Repair by
DMAPT Sensitizes Non-small-cell Lung Cancers to X-rays
N. C. Estabrook, H. Chin-Sinex, A. J. Borgmann, R. M. Dhaemers,
R. H. Shapiro, D. Gilley, N. Huda, P. Crooks, C. Sweeney, and
M. S. Mendonca

342

Cloning of metK from Actinoplanes teichomyceticus ATCC31121 andEffect of Its High Expression on Antibiotic Production
Du Yeong Kim, Yong-Il Hwang, and Sun-Uk Choi

352

Effects of pimM and pimR on the Increase of Natamycin Production
in Streptomyces natalensis
Bo-Young Jang, Young-Il Hwang, and Sun-Uk Choi

357

Diazotrophic Diversity in the Rhizosphere of Two Exotic Weed
Plants, Prosopis juliflora and Parthenium hysterophorus
N. Cibichakravarthy, R. Preetha, S. P. Sundaram, K. Kumar,
and D. Balachandar

358

Diversity Analysis of Pseudomonads in Rice Rhizosphere forfor Multifaceted Plant Growth Promotion
S. Naganandini, D. Balachandar, and K. Kumar

367

viii

Analysis and Application of Bacillus subtilis Sortases toAnchor Recombinant Proteins on the Cell Wall
Hoang Duc Nguyen, Trang Thi Phuong Phan, and W. Schumann

368

Development of a Strong Intracellular Expression System
for Bacillus subtilis by Optimizing Promoter Elements
Trang Thi Phuong Phan, Hoang Duc Nguyen, and W. Schumann

379

List of Publication: Hoang Duc Nguyen

380

The Anaerobic Digestion of Solid Organic Waste
A. Khalid, M. Arshad, M. Anjum, T. Mahmood, and L. Dawson

381

Manipulation of Ethylene Synthesis in Roots throughBacterial ACC Deaminase for Improving Nodulation in Legumes
B. Shaharoona, M. Imran, M. Arshad, and A. Khalid

389

List of Publication: Azeem Khalid

390

Changes in Bacterial Communities from Anaerobic Digesters
during Petroleum Hydrocarbon Degradation
R. E. Scherr, T. Lundaa, V. Klose, G,. Bochmann, and
A. P. Loibner

391

Changes in Iso- and n-alkane Distribution during Biodegradationof Crude Oil under Nitrate and Sulphate Reducing Conditions
M. Hasinger, K. E. Scherr, T. Lundaa, L. Bräuer, C. Zach, and
A. P. Loibner

400

Expressed Sequence tag-simple Sequence Repeat-based Molecular
Variance in Two Salicornia (Amaranthaceae) Populations
Z. L. Xu, Z. Ali, J. X. Yi, X. L. He, D. Y. Zhang, G. H. Yu,
A. A. Khan, L. A. Khan, and H. X. Ma

401

Breeding Wheat Cultivars Better Adapted to Conservation Agriculture
R. M. Trethowan, T. Mahmood, Z. Ali, K. Oldach, and A. G. Garcia

416

List of Publication: Zulfiqar Ali

417

Activity, Stability, and Structure of Metagenome-derived LC11RNase H1, a Homolog of Sulfolobus tokodaii RNase H1
T. N. Nguyen, C. Angkawidjaja, E. Kanaya, Y. Koga, K. Takano,and S. Kanaya

418

Increased Production of Azadirachtin from an Improved Methodof Androgenic Cultures of a Medicinal Tree Azadirachta indicaix

A. Juss
P. Srivastava and R. Chaturvedi

427

Effect of Culture Conditions on Synthesis of Triterpenoids in
Suspension Cultures of Lantana camara L.
P. Srivastava, V. Sisodia, and R. Chaturvedi

435

REPORT of Recent Research by Former Participant in
UNESCO International Post-Graduate University Course in Microbiology
In Vitro Culture of Jewel Orchids (Anoectochilus setaceus Blume)
Phi Thi Cam Mien, Pham Luong Hang, Nguyen Van Ket,
Truong Thi Lan Anh, Phung Van Phe, and Nguyen Trung Thanh

436

Activities of International Center for Biotechnology for 2011

447

Author Index

467

x

ACTIVITIES OF ICBiotech

ACTIVITIES OF INTERNATIONAL CENTER FOR
BIOTECHNOLOGY FOR FY2011
The International Center for Biotechnology (ICBiotech) was founded in April
1995 as an independent institute in Osaka University with a mission to pursue academic
advancement and collaborative research in biotechnology. ICBiotech has its origin fromthe “International Center of Cooperative Research Center in Microbial Engineering
Japan (ICME)” which was established in the Faculty of Engineering, Osaka University
in April 1978, through renaming to “International Center of Cooperative Research in
Biotechnology (ICBiotech)” in April 1985 with the recognition of the wide acceptanceand success of ICME’s activities and achievements.
ICBiotech is dedicated to promote international cooperation among Asian
countries in the aspects of research and educational advancement in the field of
Biotechnology by propelling academic interactions in Asian countries, and is committed
to industrial biotechnology studies by means of microbial engineering and relatedsciences, focusing on the sustainable utilization of abundant natural resources inSoutheast Asian countries.
ICBiotech serves as the seat of education and research in Asia, with the support of
the Ministry of Education, Culture, Sports, Science and Technology(Monbu-kagaku-sho, MEXT), the Japan Society for the Promotion of Science (JSPS),
and Japan Science and Technology Agency (JST) and the auspices of the United
Nations Educational, Scientific and Cultural Organization (UNESCO), with thecooperation of the Department of Biotechnology, Graduate School of Engineering,Osaka University, and researchers from prestigious universities nationwide and abroad.
In 2002 Mahidol University-Osaka University Collaborative Research Center for
Bioscience and Biotechnology: MU-OU:CRC was set up at Faculty of Science, Mahidol
University as a collaborative research center to accomplish multidisciplinary research in
the field of Bioscience and Biotechnology.
The activities of the ICBiotech include:
1. Research and Education
The main area of research is industrial biotechnology rooted in microbial
engineering, whilst centering on the sustainable use of agricultural and forest
resources in bioresource-rich countries such as those in Southeast Asia. Research
is underway in the field of cell engineering with the objectives of analyzing thecellular functions of bacteria, fungi and plants, and developing and using functionsof these cells for management and rational use of biological resources that exist on
our planet. ICBiotech covers three areas of Biotechnology:
1) Discovery of new functions from biological resources.
2) Bio-conversion and process engineering of biological resources.
3) Conservation of biological resources.
For Post-Graduate Course, ICBiotech is involved in the education activities ofDepartment of Biotechnology, Graduate School of Engineering as collaborating447

laboratories and currently covers several fields of biotechnology in education andresearch: Molecular Microbiology laboratory chaired by Prof. Takuya Nihira andApplied Microbiology laboratory chaired by Prof. Kazuhito Fujiyama.
2. Participating in FrontierLab@OsakaU ’Scientific Empowerment Program forInternational Students’ which was created for international students to conduct
thermatic studies and achieve results under the guidance of supervisors while
acquiring skills necessary for continuing research in one of Osaka University’s
internationally renowned science and technology laboratories for a period of up to
12 months.
3. Acting as collaborating laboratories with the Department of Biotechnology,
Graduate School of Engineering, Osaka University for ‘International Program of
Frontier Biotechnology’, a five-year program for master’s and doctor’s degrees.
The aim of this program is to expose graduate students (privately financed as wellas the Japanese Government Scholarship students) to state-of-the-art research skills
and in-depth knowledge of advanced biology, chemistry and physics to harness thepotential of biotechnology.
4. Having been administrating and implementing the “UNESCO Postgraduate
Inter-University Course in Biotechnology” (2004-2007) at the Department of
Biotechnology, Graduate School of Engineering, Osaka University, taking over the
long-termed “International Post-Graduate University Course in Microbiology”(1972-2003) with total of 459 alumni spreading all over Asia.
5. Undertaking a JSPS Asian CORE Program entitled as ‘Next-Generation
Bioproduction Platform Leveraging Subtropical Microbial Bioresources’. This
project aims to develop next-generation bioproduction platform by interaction
between excellent researchers from Japan, Thailand, Viet Nam, Laos and Cambodia,
together with fostering young researchers from those countries for future in the fieldof industrial biotechnology. The program is based on the result from preceding
MEXT/JST project carried out in FY2006-2008, which took over the termination in2004 of the JSPS multilateral program in the field of Biotechnology.
6. Involving as a research group at Japan side for a JICA-JST Project in Thailand:
Thailand-Japan Collaboration for the Research and Development of Therapeutic
Products against Infectious Diseases. The Research Institute for Microbial Diseases(RIMD), Osaka University, acts as the Japanese host coordinator for the project.
The Project is implemented under the “Science and Technology ResearchPartnership for Sustainable Development (SATREPS)” promoted by Japan
International Cooperation Agency (JICA) and JST in collaboration.
7.

Serving as the secretariat for the UNESCO Regional Network for Microbiology andBiotechnology in Southeast Asia in accordance with a proposed memorandum
resulted from a Consortium Meeting of the Project “Tropical Bio-Resources andGreen Chemistry Strategy” (Osaka 2003).

448

8.

Working as a member of the global network “Microbiological Resource Centers”(MIRCENs) operated by UNEP, UNESCO and ICRO; and as an original member of
the “Regional Network for Microbiology in Southeast Asia” operated under the
auspices of UNESCO through the Fund-In-Trust of the Japanese Government(J-FIT).

9. Promoting international cooperative researches in biotechnology with the Southeast
Asian countries related to Biotechnology, under the support of Monbu-kagaku-sho
Grant-in-Aids for International Scientific Research.
In addition, ICBiotech
cooperates in developing international organization and conducting academic
seminars related to biotechnology.
10. Publishing Annual Reports of ICBiotech periodically.
Steering Committee
ChairmanProf. Takuya Nihira (Director of ICBiotech)Committee Members
Prof. Kazuhito Fujiyama (ICBiotech)
Prof. Satoshi Harashima (Graduate School of Engineering)
Prof. Shigeru Kanaya (Graduate School of Engineering)
Prof. Eiichiro Fukusaki (Graduate School of Engineering)
Prof. Tsuyoshi Inoue (Graduate School of Engineering)
Prof. Kazumasa Hirata (Graduate School of Pharmaceutical Sciences)Prof. Masahito Taya (Graduate School of Engineering Science)Prof. Toshihiro Horii (Research Institute for Microbial Diseases)
Staff
Director/Professor
Professor
Interlocking Professor

Dr. Takuya Nihira
Dr. Kazuhito Fujiyama
Dr. Michihiko Ike (Department of Environmental
Engineering, Osaka University)
Collaborative Professor
Dr. Amaret Bhumiratana (Mahidol University, Thailand)
Dr. Watanalai Panbangred (Mahidol University,
Thailand)Dr. Skorn Mongkolsuk (Mahidol University, Thailand)
Visiting Professor
Dr. Suteaki Shioya (Sojo University)
Visiting Associate Professor Dr. Michiko Ito (Kyoto University)
LecturerDr. Ryo Misaki
Assistant Professor
Dr. Hiroshi Kinoshita
Dr. Shigeru Kitani
Dr. Takao Ohashi
Administrative Official
Ms. Rina Tada
Administrative Assistant
Ms. Fumiko Tomomatsu

449

Technical Assistant

Ms. Yukiko Seto
Ms. Chunxiang Wang

I . COOPERATIVE RESEARCH STATION (CRS) IN SOUTHEAST ASIA
Mahidol University (MU) and Osaka University (OU) together established theMahidol University-Osaka University Collaborative Research Center (MU-OU:CRC)
for Bioscience and Biotechnology at Faculty of Science, Mahidol University in 2002, tostrengthen the research cooperation in these fields which are amongst the most active
fields of study and research in both universities.
Currently, MU-OU:CRC has co-ordinated a research projects under the jointly
support of National Research Council of Thailand (NRCT), National Center for Genetic
Engineering and Biotechnology (BIOTEC) and The Japan Society for the Promotion of
Science (JSPS). Researchers from Mahidol University, Chulalongkorn University,
Kasetsart University, King Mongkut's University of Technology Thonburi and BIOTEC
participate in this project.
CRS is conducting researches on
1. Strain improvement of Bacillus thuringiensis.
2. Screening of actinomycetes for bioactive compound.
3. The soil metagenome for screening of potential polysaccharide-degrading
enzymes.
4. Cell culture system for screening of bioactive compounds from Streptomycetes
and environmental fungi.
5. Quorum sensing interference: Novel biocontrol strategies for pathogenic
bacteria.
II. UNESCO COURSES”UNESCO International Post-Graduate University Course in Microbiology”(1973-2003) plus the “UNESCO Postgraduate Inter-University Course in
Biotechnology” (2004-2007) was one of the two long-term training courses held inJapan launched in response to the recommendations concluded at a UNESCO General
Conference Meeting. After the operation of 33 batches for developing humanresources in Asia, total 459 young researchers from all over Asian countries are now
playing active and leading roles in current biotechnology research and education in theregion to contribute to the development of their own country, and consequently to Asian
countries, and the establishment of the closer and stronger network in the region.
UESCO Conference Series on Capability-Building in Life Sciences
In the year of 2007, the program of UNESCO Postgraduate Inter-University
Course was temporarily terminated to further evaluate and re-design with a view todelivering a new program in response to evolving needs of regional standpoints.
Under the circumstance, sponsored by UNESCO through the J-FIT of MEXT,

450

UNESCO Conference Series on Capacity-Building in Life Science was organized byholding conferences in Thailand (Bangkok, October 2009), Vietnam (Hanoi, October2009), Philippines (Manila, February 2010), Indonesia (Yogyakarta, May 2010) andfinally in Thailand (Bangkok, September 2010) as the consulting workshop with the
UNESCO Regional Network for Microbiology and Biotechnology, which resulted indesigning of new UNESCO training course for human resources development in theregion and successful proposal to UNESCO.
UNESCO Training Project – One-month Lab Revisit ProgramThis project was conducted in August - September 2011, under the Official
Development Assistance Grants for UNESCO Activities of MEXT, based on the resultof the Conference Series. Participants were alumni of the previous UNESCO courses
on Microbiology and Biotechnology, or those nominated by the senior alumni in theirhome laboratories.
Purpose
1. To provide the opportunity for the alumni and related young researchers to visit
laboratories in Japanese universities to be exposed to leading-edge of research,
to upgrade research skills, and to facilitate exchange of researchers.
2. To rebuild up the networks among researchers between Japan and Asian
countries in the field of biotechnology.
3. To strengthen the network among the alumni of our UNESCO courses beyond
generations and countries.
List of ParticipantsGroup AName

Nationality

Affiliation

Host Researcher

Past UNESCO Course

Ta Bich Thuan

Vietnam

Faculty of Biology, Hanoi
University of Science, VNU

Prof. T. Nihira,
Osaka University

UM23

Natthaporn Takpho

Thailand

Department of
Biotechnology, Faculty of
Science, Mahidol University

Prof. S. Harashima,
Osaka University

Nominated by
Dr. Chuenchit Boonchird(UM10, Osaka U)

Anchaya
Mongkolchaiyaphruek

Thailand

Department of Horticulture,
Faculty of Agriculture,
Kasetsart University

Prof. T. Muranaka,
Osaka University

Nominated by
Dr. Savitr Trakulnaleamsai(UM17, Osaka U)

Pagakrong Wanapaisan

Thailand

Department of
Biotechnology, Faculty of
Science, Mahidol University

Prof. K. Fujiyama,
Osaka University

Nominated by
Prof. Watanalai Panbangred(UM06, Osaka U)

451

Prof. E. Fukusaki,
Osaka University

Nominated by
Dr. Ukan Sukandar(UM07, Osaka U)
Dr. Sastia Prama Putri(UB02, Osaka U)

Ronny Purwadi Herman

Indonesia

Department of Chemical
Engineering, Institut
Teknologi Bandung

Darmawan Ari Nugroho

Indonesia

Faculty of Agricultural
Technology, Gadjah Mada
University

Prof. H. Ohtake
Osaka University

UB02

Cristopher Salazar
EscanoPhilippines

National Institute of
Molecular Biology andBiotechnology (BIOTECH)

Prof. K. Kitamoto,
The University of
Tokyo

UM30

Susan Afable Sedano

Philippines

National Institute of
Molecular Biology andBiotechnology (BIOTECH)

Prof. J. Ogawa,
Kyoto University

UM16

Francisco Banzuela
ElegadoPhilippines

National Institute of
Molecular Biology andBiotechnology (BIOTECH)

Prof. K. Sonomoto,
Kyushu University

UM13

K.H.M. Nazmul Hussain
Nazir

Bangladesh

Department of Microbiology
and Hygiene, Bangladesh
Agricultural University

Assoc. Prof. M.
Goto, Kyushu
University

UB02

Nguyen Quang Huy

Vietnam

Faculty of Biology, Hanoi
University of Science, VNU

Prof. S. Kanaya,
Osaka University

UM25

Karseno bin Mulsidi

Indonesia

Department of Agricultural
Technology, Jenderal
Soedirman University

Prof. T. Nihira,
Osaka University

UM29

Neung Teaumroong

ThailandResearch Department,
Institute of Agricultural
Technology, Suranaree
University of Technology

Prof. M.
Minamisawa,
Tohoku University

UM17

Narayan Roy

Bangladesh

Department of Biochemistry
and Molecular Biology,
University of Rajshahi

Prof. K. Abe,
Tohoku University

UM22

Nguyen Thi Bich
PhuongVietnamInnovGreen Corporation

Prof. K. Sonomoto,
Kyushu University

UM30Group B

UM: International Post-Graduate University Course in Microbiology
UB: International Inter-University Course in Biotechnology

Schedule[Group A]:

August 1
August 3
August 29

Arrive at Osaka
Move to assigned laboratories
Discussion and report submission in Osaka University452

[Group B]:

August 30
Depart from Osaka
August 25
Arrive at Osaka
August 30
Move to assigned laboratories
September 22 Report submission
September 23 Depart from Osaka

Program of Lectures and Discussion
August 29, 2011
Venue: Suntory Memorial Hall, Osaka University
10:00-10:15 Opening remarks by
Prof. S. Harashima, Professor, Department of Biotechnology, Graduate School ofEngineering, Osaka University.
Prof. T. Nihira, Director of ICBiotech, Osaka University.
10:15-10:45 Self introduction from each participant of the program.
10:45-11:15 ‘Novel method for measuring active tension generation by skeletal muscle cells andits application’ by Dr. E. Nagamori, Associate Professor, Department of
Biotechnology, Graduate School of Engineering, Osaka University.
11:15-11:30 Instruction by organizer.
11:30-13:30 Lunch break.
Venue: GSE common meeting room
13:30-14:00 ‘Replicating the biosynthetic pathways in medicinal plants for the production of
useful compounds’ by Dr. Hikaru Seki, Associate Professor, Department of
Biotechnology, Graduate School of Engineering, Osaka University.
14:00-14:30 ‘Molecular breeding and bioresosurces in yeasts’ by Dr. Y. Kaneko, Professor,
Department of Biotechnology, Graduate School of Engineering, Osaka University.
14:30-14:45 Break.
14:45-16:45 Discussion on the previous/new UNESCO courses.
17:30Reception party

UNESCO Biotechnology School in Asia (JFIT Project)
Taking over the termination of 34-year-operation of the two long-term UNESCO
courses held in Japan, UNESCO Biotechnology School in Asia will newly launch in2013 as an activity of the “UNESCO Extra-budgetary Program for Human Resources
and Research Network in Sciences and Technology” supported by the JapaneseFund-In-Trust (JFIT). It aims to train Asian university-graduates in the field of
Biotechnology and related areas resulting in achieving master degree, and theparticipants are expected to make significant contributions to an educational orscientific institution in the coming years in their home countries.
The proposal of the Project was officially approved on March 5, 2012. The
2013 Course consists of the 2-year Master-degree awardable courses(August/September 2013 – July/August 2015). Participants from Cambodia, Laos,
Myanmar or Mongolia etc will enroll into the master courses of universities in453

Indonesia, Thailand or Vietnam. During the first year of the relevant master course,
participants from Asian countries will be equipped with contemporary expandingknowledge and technology for further development of skills and study for advanced
research following the curricula of the relevant master courses.
III. JSPS ASIAN CORE PROGRAM: NEXT-GENERATION BIOPRODUCTION
PLATFORM LEVERAGING SUBTROPICAL MICROBIAL
BIORESOURCES (2009-2013)
Following the termination of the JSPS multilateral program in the field of
Biotechnology under the framework of Core-University System conducted for the past
27 years since 1978, a scheme of collaboration of Japan Science and Technology
Agency (JST) was carried out in FY2006-2008 as a program of "Solution of Research
Issues Shares by Asian Region” under the framework of “Asian Science andTechnology Cooperation Promotion Strategy" supported by the Special Coordination
Funds for Promotion of Science and Technology (SCF) of MEXT.
This project aims to develop next-generation bioproduction platform byinteraction between excellent researchers from Japan, Thailand, Viet Nam, Laos andCambodia, together with fostering young researchers from those countries for future in
the field of industrial biotechnology.
1. Purpose
Based on the result from preceding MEXT/JST project carried out in
FY2006-2008, the project area is expanded widely to Thai neighbouring countries (VietNam, Laos and Cambodia) in addition to Thailand, and research exchange will be
carried out focusing on development of bioproduction platform using microbes.
Thailand, Viet Nam, Laos and Cambodia belong to countries having subtropicalclimate, and thus have abundant bioresources, such as agro/marine resources. Especially,
microbial resources as invisible bioresources are highly abundant, possessing diverse
array of unexplored microorganisms which are not present in Japan. These novel usefulbioactive compounds (such as antibiotics and bacteriocins), producers of usefulindustrial enzymes (phytase of soft biomass degrading enzymes), whole-cell catalyst to
produce bioethanol or sugars through degradation of soft biomass or the biocatalyst
replacing present toxic inorganic catalyst in industrial processes in convertinghydrophobic compounds. On-site bioproduction platform using Southeaset Asianbioresources is essential as the next-generation fundamental technology. This project
aims to develop next-generation bioproduction platform by interaction between
excellent researchers from Japan, Thailand, Viet Nam, Laos and Cambodia, fostering
young researchers from those countries for future in the field of industrial
biotechnology.
2. Outline of Implementation System454

Japanese academic consortium will be formed from professional academic staffs
belonging to top five Japanese universities (Osaka University, The University of Tokyo,
Hokkaido University, Kyushu University and Toyama Prefectural University) who are
excellent in several steps of industrial biotechnology. Similarly, Thai academic
consortium will be formed from representative young researchers in the field ofbiotechnology from Thai top 4 universities (Mahidol University, Chulalongkorn
University, Kasetsart University, and King Mongkut’s University of Technology
Thonburi) and BIOTEC (National Center for Genetic Engineering and Biotechnology).
The international collaboration will be performed between the Japanese consortium andthe Thai consortium to design and establish efficient way in the utilization ofbioresources.
Japanese core organization: Osaka University.
Japanese coordinator: Prof. Takuya Nihira, ICBiotech, Osaka University.
Counterpart country and core organization:
Prof. Watanalai Panbangred, Mahidol University, Thailand.
Dr. Duong Van Hop, The Institute of Microobiology and Biotechnology,
Viet Nam National University, Hanoi, Viet Nam.
Mr. Sokunthia Thao, Royal University of Phnom Penh, Cambodia.
Dr. Sundara Souriuodong, Science and Technology Research Institute,
National Authority for Science and Technology, Laos.
3. Research Topics and Participating Members (*Group leader)Group 1. Production of novel bioactive compounds
Japan:

Takuya Nihira（ICBiotech, Osaka University）*
Eiichiro Fukusaki (Osaka University)
Kazumasa Hirata (Osaka University)
Yasuhiro Igarashi (Toyama Prefectural University)
Kenji Sonomoto（Kyushu University）
Thailand: Watanalai Panbangred (Mahidol University)
Amaret Bhumiratana (Mahidol University)
Patoomratana Tuchinda (Mahidol University)
Amonlaya Tosukhowong (BIOTEC: National Center for Genetic Engineeringand Biotechnology)
Wonnop Visessanguan (BIOTEC: National Center for Genetic Engineering andBiotechnology)
Ruud Valyasevi (BIOTEC: National Center for Genetic Engineering andBiotechnology)
Vietnam: Duong Van Hop (Institute of Microbiology and Biotechnology (IMBT),
Vietnam National University Hanoi)
Cambodia: Hang Chan Thon (Royal University of Phnom Penh)
Thao Sokunthia (Royal University of Phnom Penh)
Laos:
Sourioudong Sundara (Laos National Authority of Science and Technology)

Group 2. Production of novel useful enzymes
Japan:

Kazuo Sakka（Mie University）*
Kouzo Asano (Hokkaido University)455

Thailand:

Vithaya Meevootisom (Mahidol University)
Suthep Wiyakrutta (Mahidol University)
Khanok Ratanakhanokchai (King Mongkut's University of Technology
Thonburi)Vietnam: Duong Van Hop (Institute of Microbiology and Biotechnology (IMBT),
Vietnam National University Hanoi)Group 3. Development of novel biocatalysts
Japan:

Thailand:

Satoshi Harashima（Osaka University）*
Hisao Ohtake （Osaka University)
Kazuhito Fujiyama (Osaka University)
Yasuo Igarashi（The University of Tokyo）
Savitree Limtong (Kasetsart University)
Wichien Yongmanitchai（Kasetsart University）
Chuenchit Boonchird (Mahidol University)
Thipa Asvarak (Mahidol University)
Thunyarat Pongtharangkul (Mahidol University）
Alisa Vangnai (Chulalongkorn University)
Verawat Champreda (BIOTEC: National Center for Genetic Engineering andBiotechnology)

4. Record of Scientist Exchange
From Japan to counterpart countries
Name

Affiliation

Visiting Period

Oct. 29-Nov. 3, 2011
Jan. 29-Feb. 4, 2012
Feb. 12-18, 2012

Laos National Authority ofSci. and Technol, Laos
Inst. of Microbiol. andBiotechnol., Vietnam National
Univ., Hanoi, Vietnam
Mahidol Univ., Thailand
Mahidol Univ., Thailand
Mahidol Univ., Thailand

Mar. 1-3, 2012

Mahidol Univ., Thailand

Jan. 30-Feb. 4, 2012

Seminar in Thailand

Jan. 30-Feb. 2, 2012

Seminar in Thailand

Oct. 16-21, 2011

Mahidol Univ., Thailand

Jan. 30-Feb. 3, 2012
Oct. 20-29, 2011

Seminar in Thailand
King Mongkut’s Univ. ofTechnol., Thailand

Jan. 30-Feb. 3, 2012

Seminar in Thailand

June 12-19, 2011
Oct. 5-9, 2011
Takuya Nihira

Kazuhito Fujiyama
Satoshi Harashima

Teruo Sone

Kazuo Sakka

Director, Professor,
ICBiotech, Osaka Univ.

Professor, ICBiotech,
Osaka Univ.
Professor, Graduate
School of Engineering,Osaka Univ.
Assoc. Professor,
Graduate School of
Agriculture, Hokkaido
Univ.

Professor, Faculty of
Bioresources, Mie Univ.

Host Organization

456

Yasuo Igarashi

Yasuhiro Igarashi

Hirofumi Akiyama

Wu Lin Ghai

Hisao Otake

Hiroshi Kinoshita

Professor, Graduate
School of Agriculture,Jan. 30-Feb. 2, 2012
Univ. of Tokyo
Professor, Biotechnology
Research Center,
Jan. 29-Feb. 3, 2012
Toyama Prefectural
Univ.
Ph.D. student,
Biotechnology ResearchJan. 29-Feb. 3, 2012
Center, Toyama
Prefectural Univ.
Ph.D. student,
Biotechnology ResearchJan. 29-Feb. 3, 2012
Center, Toyama
Prefectural Univ.
Prof., Graduate Schoolof Engineering, Osaka
Jan. 30-Feb. 3, 2012
Univ.
Assist. Prof., ICBiotech,
Osaka Univ.

Mar. 11-17, 2012

Takao Ohashi
Kohsuke Honda

Takeshi Bamba

Makoto Imura

Kenji Sonomoto

Makoto Sasaki

Assist. Prof., ICBiotech,
Osaka Univ.

Assist. Prof., ICBiotech,
Osaka Univ.
Assoc. Prof., Graduate
School of Engineering,Osaka Univ.
Assoc. Prof., Graduate
School of Engineering,Osaka Univ.
Student, Graduate Schoolof Engineering, Osaka
Univ.
Prof., Graduate School of
Bioscience andBio-environmental
Sciences, Kyushu Univ.
Student, Graduate Schoolof Bioscience andBio-environmental
Sciences, Kyushu Univ.

Seminar in Thailand

Seminar in Thailand

Seminar in Thailand

Seminar in Thailand

Inst. of Microbiol. andAug. 18-Sept. 1, 2011 Biotechnol., Vietnam National
Univ., Hanoi, Vietnam
Feb. 29-Mar. 1, 2012

Shigeru Kitani

Seminar in Thailand

Inst. of Microbiol. andBiotechnol., Vietnam National
Univ., Hanoi, Vietnam
Inst. of Microbiol. andBiotechnol., Vietnam National
Univ., Hanoi, Vietnam

Jan. 8-15, 2012

Mahidol Univ., Thailand

Jan. 16-22, 2012

Royal Univ., Phnom Penh,
CambodiaJan. 29-Feb. 5, 2012

Seminar in Thailand

Jan. 30-Feb. 4, 2012

Chulalongkorn Univ., Thailand

Jan. 30-Feb. 3, 2012

Seminar in Thailand

Jan. 30-Feb. 4, 2012

Seminar in Thailand

Jan. 30-Feb. 2, 2012

Seminar in Thailand

Jan. 15-Feb. 12, 2012

BIOTEC, Thailand

457

Ryoji Yokohata

Student, Graduate Schoolof Bioscience andJan. 15-Feb. 12, 2012
Bio-environmental
Sciences, Kyushu Univ.

BIOTEC, Thailand

Between counterpart countries:
Name

Affiliation

Visiting Period

Host Organization

Pel Pisey

Research Assistant,
Royal Univ. Phnom Penh,
CambodiaOct. 1-27, 2011

Mahidol Univ., Thailand

Jan. 27-Feb. 25,
2012

Mahidol Univ., Thailand

Oct. 1-27, 2011

Mahidol Univ., Thailand

Jan. 29-Feb. 2,
2012

Mahidol Univ., Thailand

Jan. 27-Mar. 20,
2012

Mahidol Univ., Thailand

Shihavong
Khamphachan
SundaraSourioudong
Keo Phommavong

Researcher, Laos National
Authority of Science andTechnology, Laos
Director,
Laos National Authority of
Science and Technology, Laos
Researcher, Laos National
Authority of Science andTechnology, Laos

Thao Sokunthia

Dept. Chairman, Royal Univ.
Phnom Penh, Cambodia

Jan. 29-Feb. 3,
2012

Mahidol Univ., Thailand

Min Malay

Lab. Assistant, Royal Univ.
Phnom Penh, Cambodia

Jan. 29-Feb. 3,
2012

Seminar in Thailand

5. Seminar and Symposium
The Young Scientist Seminar (YSS) and a Joint Symposium (JS) scheduled in
October 2011 were held on January 31 and February 1 2012, respectively, due to thesevere floods in Bangkok.
The Young Scientist Seminar 2011
Bioresources: Their Potentials & Applications
The Asian Core Program (2009 – 2013)“Next-Generation Bioproduction Platform
Leveraging Subtropical Microbial Bioresources”
Date: January 31, 2012
Venue: Room K102, Chalermprakiet (K) Building, Faculty of Science, Mahidol University, 272
Rama VI Road, Ratchathewi, Bangkok 10400, Thailand
Hosted by: Mahidol University, Faculty of Science (MU⋅SC)In Association with:
Osaka University (OU)
National Science and Technology Development Agency (NSTDA), Thailand
National Research Council of Thailand (NRCT)
Japan Society for the Promotion of Science (JSPS)

458

Committee
Advisory Committee
Prof. Skorn Mongkolsuk, Dean, Faculty of Science, Mahidol University, Thailand
Prof. Yasuo Igarashi, The University of Tokyo, Japan
Prof. Kenji Sonomoto, Kyushu University, Japan
Dr. Douong Van Hop, Director, Institute of Microbiology and Biotechnology, Vietnam
National University Hanoi, Vietnam
Dr. Sourioudong Sundara, Director, Science and Technology Research Institute, National
Authority of Science and Technology, Laos PDR
Mr. Thao Sokunthea, Head, Department of Biology, Royal University of Phnom Penh,
CambodiaProf. Vithaya Meevootisom, Head, Department of Microbiology, Faculty of Science,
Mahidol University, Thailand
Assoc. Prof. Chuenchit Boonchird, Head, Department of Biotechnology, Faculty of
Science, Mahidol University, Thailand
Scientific Committee
Prof. Takuya Nihira (OU), Prof. Vithaya Meevootisom (MU), Prof. Yasuo Igarashi(UTokyo), Prof. Watanalai Panbangred (MU), Prof. Kenji Sonomoto (Kyushu U), Prof.
Savitree Limtong (Kaset U), Prof. Kazuhito Fujiyama (OU), Assoc. Prof. Khanok
Ratanakhanokchai (KMUTT)
Organizing Committee
Prof. Takuya Nihira (OU), Prof. Watanalai Panbangred (MU), Dr. Suthep Wiyakrutta(MU), Dr. Arjaree Nilavongse (MU), Dr. Idsada Mungsantisuk (MU), Dr. Chartchai
Changsen (MU), Dr. Soraya Chaturongakul (MU), Dr. Radeekorn Akkarawongsapat (MU),
Ms. Duangnate Isarangkul Na Ayudhaya (MU), Ms. Monludee Phianlertloy (MU), Ms
Sarucha Sankunha (MU)
Secretariat
Thai Side:
Dr. Suthep Wiyakrutta, Department of Microbiology, Faculty of Science, Mahidol
University, Bangkok, 10400, Thailand
Tel: +66-2-201-5543 Fax: +66-2-644-5411 E-mail: scsvy@mahidol.ac.th
Ms. Monludee Phianlertloy, Ms. Sarucha Sankunha, MU-OU:CRC, Faculty of Science,
Mahidol University, Bangkok, 10400, Thailand
Japanese Side:
Prof. Takuya Nihira, International Center for Biotechnology, Osaka University, Japan
Tel: +81-6-6879-7455 Fax: +81-6-6879-7454 E-mail: nihira@icb.osaka-u.ac.jp
ProgramJanuary 31, 2012
8:30 – 9:00 Registration
9:00 – 9:10 Opening Ceremony
Welcome Address by Prof. Skorn Mongkolsuk, Dean, Faculty of Science, Mahidol
University, Thailand
Morning Session
Chair person:
Prof. Kenji Sonomoto
Co-chair:
Assoc. Prof. Khanok Ratanakhanokchai
09:10 – 09:40
Keynote Lecture 1 (K-1) Prof. Yasuo Igarashi, The University of Tokyo
09:40 – 09:55
Oral Presentation 1 (O-1) Yasin Kitichantaropas (Thailand)
09:55 – 10:10
Oral Presentation 2 (O-2) Makoto Imura (Japan)
10:10 – 10:30
Coffee Break
10:30 – 10:45
Oral Presentation 3 (O-3) Paripok Phitsuwan (Thailand)
Chair person:
Prof. Kazuhito Fujiyama459

Co-chair:Dr. Sourioudong Sundara
10:45 – 11:00
Oral Presentation 4 (O-4) Ryoji Yokohata (Japan)
11:00 – 11:15
Oral Presentation 5 (O-5) Wuttichai Mhuantong (Thailand)
11:15 – 11:30
Oral Presentation 6 (O-6) Hirofumi Akiyama (Japan)
11:30 – 11:45
Oral Presentation 7 (O-7) Notthaporn Phrueksahiran (Thailand)
11:45 – 12:00
Oral Presentation 8 (O-8) Linkai Yu (Japan)
12:00 – 14:00
Lunch & Poster Presentation
Afternoon Session
Chair person:
Prof. Yasuo Igarashi
Co-chair:Prof. Savitree Limtong
14:00 – 14:30
Keynote Lecture 2 (K-2) Prof. Kenji Sonomoto, Kyushu University
14:30 – 14:45
Oral Presentation 9 (O-9) by a Thai young scientist
14:45 – 15:00
Oral Presentation 10 (O-10) Makoto Sasaki (Japan)
15:00 – 15:15
Oral Presentation 11 (O-11) by a Thai young scientist
15:15 – 15:35
Coffee Break
Chair person:
Dr. Douong Van Hop
Co-chair:Mr. Thao Sokunthea
15:35 – 15:50
Oral Presentation 12 (O-12) by a Thai young scientist
15:50 – 16:05
Oral Presentation 13 (O-13) by a Thai young scientist
16:10 – 16:30
Closing CeremonyAward Presentation
Closing Remark by Prof. Takuya Nihira
Keynote Lectures & Oral Presentations
K-1 Prof. Yasuo Igarashi, The,University of Tokyo“From Pure Culture to Mixed Culture-New Aspects of Biotechnology-“
K-2 Prof. Kenji Sonomoto, Kyushu University“New Era of Lactic Acid Bacteria to Open the Future”
O-1 Yasin Kitichantaropas, Mahidol University“Oxidative stress caused by environmental stresses in thermotolerant yeast”
O-2 Makoto Imura, Osaka University“Identification of replicon of a 111-kb circular plasmid from the hydrophobic bacterium
Rhodococcus opacus B-4”
O-3 Paripok Phitsuwan, King Mongkut's University of Technology Thonburi“Improved deconstruction of natural plant biomass by a combination of commercial cellulases
and hemicellulases from Tepidimicrobium xylanilyticum BT14”
O-4 Ryoji Yokohata, Kyushu University“Antagonist design of gelatinase biosynthesis-activating pheromone of Enterococcus faecalis
based on reverse alanine scanning”
O-5 Wuttichai Mhuantong, BIOTEC“Bioinformatic analysis and comparative study of metagenomes of lignocellulosic biomass
degrading microbial communities”
O-6 Hirofumi Akiyama, Toyama Prefectural University“Linfuranones A and B, novel polyketides from an endophytic Microbispora sp. GMKU363”
O-7 Notthaporn Phrueksahiran, Mahidol University“Screening and identification of antifungal metabolites from Streptomyces spp.”
O-8 Linkai Yu, Toyama Prefectural University“Jommthonic acid, a novel bioactive compound from Streptomyces in Thailand”
O-9 Thai Young Scientist
O-10 Makoto Sasaki, Kyushu University“Purification and characterization of ABC transporter NukT”
O-11 Thai young scientist
O-12 Thai young scientist
O-13 Thai young scientist

460

Poster Presentations (P-1 ~ P-14)
Code Presenter Title
P-1 Rattiya Waeonukul, King Mongkut's University of Technology Thonburi“Development of efficient biomass saccharification using highly active multienzyme complexes”
P-2 Thai young scientist, Mahidol University“Screening of actinomycetes from plant rhizospheric soils with inhibitory activity against
Colletotrichum spp.”
JSPS Asian CORE Symposium 2011
on
Bioproduction Platform Leveraging Subtropical Microbial Bioresources
Date: February 1, 2012
Venue: The Imperial Queen’s Park Hotel
Program
From To
14:00 14:02

1

Welcome address Prof. Takuya Nihira (Osaka U)/ Prof. Watanalai Panbangred(Mahidol U)
14:02 14:05
2 Address NRCT or NSTDA (Tentative)
14:05 14:08
3 Address JSPS Bangkok (Tentative)
Chaired by Prof. Hisao Ohtake (Osaka U)
14:08 14:29
4 Detection and characterization of putative novel lactococcal bacteriocins
Dr. Wonnop Visessanguan (BIOTEC)
Dr. Amonlaya Tosukhowong (BIOTEC)
Prof. Kenji Sonomoto (Kyushu U)
14:29 14:50 5 Drug lead discovery from microorganisms in Thailand
Prof. Watanalai Panbangred (Mahidol U)
Prof. Yasuhiro Igarashi (Toyama Pref U)
Prof. Takuya Nihira (Osaka U)
14:50 15:11 6 Xylanolytic enzymes of Paenibacillus curdlanolyticus B-6 applicable tobiomass degradation and xylooligosaccharides production
Dr. Khanok Ratanakhanokchai (KMUTT)
Prof. Kazuo Sakka (Mie U)
15:11 15:32 7 Phytases and polysaccharide hydrolases from microbes and metagenomes
Prof. Vithaya Meevootisom (Mahidol U)
Prof. Kozo Asano (Hokkaido U)
Assoc. Prof. Teruo Sone (Hokkaido U)
15:32 16:00 Coffee Break
Chaired by Assoc. Prof. Takeshi Bamba (Osaka U)
16:00 16:22 8 Development of host vector system in a oleaginous yeast Rhodosporidium
toruloides DMKU3-TK16 and screening of other oleaginous yeasts showing
high glycerol assimilation
Prof. Savitree Limtong (Kasetsart U)
Prof. Kazuhito Fujiyama (Osaka U)
Assist. Prof. Takao Ohashi (Osaka U)
Prof. Eiichiro Fukusaki (Osaka U)
16:22 16:44
9 Breeding of superior yeast bioethanol producer with Thai origin displaying
multiple stress tolerance
Dr. Chuenchit Boonchird (Mahidol U)
Prof. Satoshi Harashima (Osaka U)
16:44 17:06 10 Development of a whole-cell biocatalyst expressing the P450 monooxygenase
for synthesis of epoxyhexane461

Dr. Thunyarat Pongtharangkul (Mahidol U)
Dr. Alisa Vangnai (Chulalongkorn U)
Prof. Hisao Ohtake (Osaka U)
Dr. Kohsuke Honda (Osaka U)
17:06 17:28 11 Application of biomass-specific thermophilic microbial consortia forsaccharification and production of biorefinery products from local agricultural
biomass
Dr. Verawat Champreda (BIOTEC)
Prof. Yasuo Igarashi (U Tokyo)
17:28 17:30 12 Closing remark/Asian CORE project in the future
Prof. Takuya Nihira (Osaka U)
Watanalai Panbangred (Mahidol U)

6. Committee Meeting
July 5, 2011. Domestic Member meeting at ICBiotech, Osaka University, attended
by Japanese cooperative researchers.
January 30, 2012. Coordinator meeting in Bangkok Seminar place, The Imperial
Queen’s Park Hotel..
January 16, 2012. Domestic Member meeting in Japan at ICBiotech, Osaka
University, attended by Japanese cooperative researchers.
IV. JSPS Bilateral Program of Joint Research Project and Seminars - Vietnam(August 2011-March 2014)
JSPS promotes international scientific exchanges between Japan and counterpart
countries in accordance with agreements or memoranda of understanding concluded
with academies, research councils and other science-promotion organizations in thosecountries. ICBiotech participates in the bilateral programs agreed between Vietnam
Academy of Science and Technology (VAST), which mainly take the form of joint
research projects, joint seminars and researcher exchanges.
V. JASSO SS&SV Program (April 2011-March 2012)
Short-term stay and short-term visit program (SS&SV) by the Japan Student
Services Organization (JASSO) is a program of both student acceptance and dispatch by
Japanese universities. It must be conducted under each Japanese university’s control
and responsibility. JASSO provides selected students with monthly stipend of lessthan 3 months. In 2011, Japanese MS students stayed in universities in Thailand andVietnam, whereas 6 students from Thailand and Vietnam conducted research for 3
weeks, September 5-26, 2011, in the Prof. Fujiyama’s laboratory of ICBiotech,cooperated by Prof. Nihira (ICBiotech) and Prof. Harashima (Dept. Biotechnology,
Osaka University), as follows:
From Thailand: Thunnalin Winuprasith (Mahidol University), Narichsara Buasri (Mahidol
University), Junjira Sae-Tang (King Mongkut’s University of Technology), Phraewpan

462

Leangapichart (King Mongkut’s University of Technology).
From Vietnam: Vu Minh Man (Cao Tho University), Tran Van Thong (Cao Tho University)

VI. JST-JICA (SATREPS) (2008-2012)
SATREPS (Science and Technology Research Partnership for Sustainable
Development) is a Japanese government program that promotes international joint
research targeting global issues. SATREPS projects are expected to lead to outcomeswith potential for practical utilization, and to enhance research capacity in thedeveloping country. The program is conducted through collaboration between two
Japanese government agencies: the Japan Science and Technology Agency (JST) andthe Japan International Cooperation Agency (JICA).
ICBiotech works as a cooperative group to the following project.
Topic: Research and Development of Therapeutic Products against Infectious Diseases,
especially Dengue Virus Infection Creating Drugs Effective against the Dengue
Virus from Human Beings.
Principal Investigator: Prof. IKUTA Kazuyoshi/Research Institute for Microbial
Diseases, Osaka University.
Research Period: 4 years from 2008.
Counterpart Research Institutions: Ministry of Public Health-National Institute of
Health (NIH), Mahidol University (Thailand).
Research Project:
Preventing the further spread of dengue fever in Southeast Asia. Dengue fever isa viral disease spread by mosquitoes that infects 50 million people living in the tropics
every year, with 250,000 suffering seriously as a result, but no effective therapies have
yet been discovered. Researches are for studying patients and microorganisms fromThailand to contribute to the development of drugs effective against dengue fever. Since
the human body creates proteins (antibodies) to combat the dengue virus, investigatingthese proteins to find any that appear particularly effective against the virus and also
searching microorganisms prevalent in Thailand for compounds that block the virus.
Conducting experiments to develop new drugs following the successful creation of
antibodies. Having succeeded in creating many human-derived antibodies against thedengue virus, and also setting a course of action for research on influenza virus andbotulinum toxin, the project will conduct animal and other experiments to assess theeffectiveness of antibodies showing the most potential with the aim of developing new
therapeutic drugs.
VII. FIELDO Program (2011~ )Fieldwork, Internship and Experiential Learning Design Office (FIELDO) wasestablished in the Global Collaboration Center (GLOCOL), Osaka University in 2010 tooffer fieldwork, internship and study tours programs. As a coordinating institution,
ICBiotech arranged six graduate students (as follows) to conduct research in the463

laboratory of Dr. Yoshinori Sumimura, Division for Educational Development,
GLOCOL, Osaka University, during September 2-25, 2011.
From

Name
Ms. Penporn Sae-Tang

Ms. Thongpan Leangapichart

Mr. Chonprakun Thagun
Mr. Wasin Buasri
Ms. Khong Thi Diep
Ms. Tran Thi Thu Suong

School of Bioresources and Technology, King
Mongkut’s University of Technology, Thonburi,
Thailand
School of Bioresources and Technology, King
Mongkut’s University of Technology, Thonburi,
Thailand
Department of Biotechnology, Faculty of Science,
Mahidol University, Thailand
Department of Biotechnology, Faculty of Science,
Mahidol University, Thailand
Thai Binh Medical University, Vietnam
Cao Tho University, Vietnam

VIII. VISITORSJapanese Visiting Professor and Visiting Associate Professor
Visiting Professor
Dr. Suteaki Shioya, Sojo University
Visiting Associate Professor Dr. Michiko Ito, Kyoto University
Visiting Research Scholar
Name

Affiliation

Staying Period

Punchapat Sojikul

Mahidol University, Thailand

2011.4.5-2011.6.3

Niran Roongsawang

National Center for Genetic Engineering
2011.6.20-2011.9.14
and Biotechnology, Thailand

MarasriRuengjitchatchawalya

King Mongkut’s University ofTechnology Thonburi, Thailand
King Mongkut’s University ofTechnology Thonburi, Thailand

2011.9.1-2011.9.30

Irfan Dwidya Prijambada

Gadjah Mada University, Indonesia

2012.2.1-2012.2.29

Kanokwan Poomputsa

2011.7.1-2011.8.31

Research Fellow
Name

Affiliation

Nguyen Huynh Minh Quyen

Vietnam National University, Vietnam

2011.2.28-2011.4.2

Qusana Boonlucksanawong

Mahidol University, Thailand

2011.6.11-2011.7.10

Panadda Dhepakson
Apichai Prachasupsap

Medical Biotechnology Center,
Ministry of Public Health, Thailand
Medical Biotechnology Center,
Ministry of Public Health, Thailand

464

Staying Period

2011.7.3-2011.7.30
2011.7.3-2011.7.30
2012.2.20-2012.2.27

Pannamthip Pitaksajikul

Mahidol University, Thailand

2011.12.12-2011.12.19

Anicha Leuungchaichaweng

Medical Biotechnology Center,
Ministry of Public Health, Thailand

2012.2.20-2012.2.27

Other Guests/Visitors
Name

Affiliation

Staying Period

Alisa S. Vangnai
Nguyen Anh Tuan
Le Viet Dung

Indian Institute of Technology-Delhi,
India
Can Tho University, Vietnam
Can Tho University, Vietnam
Can Tho University, Vietnam
Can Tho University, Vietnam
Nha Trang Pasteur Inst., Vietnam
Nha Trang Pasteur Inst., Vietnam
Binh Dien Whole Sale Market Co.,
VietnamBinh Dien Whole Sale Market Co.,
VietnamBogor Agricultural University,
Indonesia
Bogor Agricultural University,
Indonesia
King Mongkut’s University Thonburi,
Thailand
Chulalongkorn University, Thailand
Can Tho University, Vietnam
Can Tho University, Vietnam

Nguyen Van Be

Can Tho University, Vietnam

2011.11.8

Tran Thi Thanh Hien

Can Tho University, Vietnam

2011.11.8

Nguyen Mih Tri

Can Tho University, Vietnam

2011.11.8

Raymond L. Rodriguez

University of California, Davis, USA

2011.12.15

Irfan D. Prijambada

Gadjah Mada University, Indonesia
National Institute of Education
Management, Vietnam
Ministry of Science and Technology,
Vietnam
Ministry of Science and Technology,
Vietnam
Ministry of Science and Technology,
Vietnam
Ministry of Science and Technology,
Vietnam
Ministry of Science and Technology,
Vietnam
Ministry of Science and Technology,
VietnamNational Academy of Agricultural
Science, Korea
University of California, Davis, USA
Vietnam Academy of Science andTechnology, Vietnam
DOST, Philippines

2011.12.19

Virendra Swarup Bisaria
Nguyen Thanh Phuong
Nguyen Cong Ha
Nguyen Trong Ngu
Le Nguyen Doan Duy
Nguyen Thi Ngoc Hue
Dao Thi Van Khanh
Ho Phuoc Hai
Truong Van Nhut
Anas M. Fauzi
Rinekso Sukmadi
Sakarindr Bhumiratana

Le Phuoc Minh
Nghiem Xuan Minh
Le Minh Sat
Nguyen Phuck Khanh
Ngo Sy Duoc
Dang Guoc Bao
Phan Hong Duong
Soo-Hwan Yeo
Dewey D. Y. Ryu
Hoang Le Tuan Anh
Ermie Bacarra

465

2011.6.3
2011.6.20
2011.6.20
2011.6.20
2011.6.20
2011.6.20
2011.6.20
2011.6.20
2011.6.20
2011.7.25
2011.7.25
2011.10.4
2011.10.162011.11.8
2011.11.8

2011.12.21
2011.12.21
2011.12.21
2011.12.21
2011.12.21
2011.12.21
2011.12.21
2011.12.22
2012.2.9
2012.2.13
2012.2.1

Foreign students enrolled in ICBiotech
Japanese Government (Monbukagakusho) Scholarship:
Nguyen Havy (Vietnam National University, Ho Chi Minh City, Vietnam)
Ivy Grace U. Pati (BIOTECH, University of the Philippines at Los Banos, Philippines)
Mustafa Nabilah Sari (Gadjah Mada University, Indonesia)
International Program of “Frontier Biotechnology” for short term:
Nuttida Teerawattanakijipakorn (Thailand)
Privately financed:
Nguyen Viet Thi Minh (Hanoi University of Agriculture, Vietnam)
Ly Nhung Hong (Vietnam National University, Ho Chi Minh City University ofTechnology, Vietnam)
Mario Rovani Hendriyento (Indonesia)
IX. COMMITTEE MEETING
Steering Committee Meetings of ICBiotech were convened as follows:
May 19, 2011
Discussion on inviting a Visiting Research Scholar: Dr. Marasri
Ruengjitchatchawalya, King Mongkut’s University of Technology, Thailand(September 1-30, 2011).
November 17, 2011
Discussion on inviting a Visiting Research Scholar: Dr. Irfan Dwidya Prijambada,
Gadjah Mada University, Indonesia (February 1-29, 2012).
Discussion/Approval on the concurrent position of Professors T. Nihira and K.
Fujiyama in International College, Osaka University (November 1, 2011-March
31, 2012).
March 16, 2012
Report on:
Renovation of ICBiotech building.
JSPS Core University Program for Cooperative Research in Asian countries.
JICA-JST Cooperative Project.
UNESCO Biotechnology School in Asia/Training Project.
UNESCO/ODA Project.
JASSO SS&SV Program.
Discussion on:
Renewal of Steering Committee members.
Conferring the title on visiting professors from abroad for 2012.
Conferring the title on visiting professors assigned from Japanese universities.

466