ANNUAL REPORTS
OF
INTERNATIONAL CENTER FOR BIOTECHNOLOGYOSAKA UNIVERSITY
VOL. 40, 2018

DIRECTOR/EDITOR

KAZUHITO FUJIYAMAASSISTANT EDITORSHIGERU KITANIRYO MISAKISECRETARYREIKO MATSUMOTOFUMIKO TOMOMATSUCHIYOKO KATAYAMAYURIKO UDA

The Annual Report is published to record the activity of the International
Center for Biotechnology (ICBiotech) and issued once in each fiscal year.
It contains scientific articles, progress reports, letters, and announcementfrom the Center. This volume includes publications by the former
participants in UNESCO courses. The editor welcomes the submission of
appropriate articles from all persons who are concerned with the activity of
the Center. All the contributions, however, will be reviewed by editors
before their acceptance. The scientific paper herein should be treated as
personal communications and not treated as original publications. The
Annual Report is distributed upon request to the International Center for
Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871,
Japan (e-mail: info_icbio@icb.osaka-u.ac.jp).

CONTENTS
Enhancement of Glycosylation by Stable Co-expression of Two Sialylation-related Enzymes on Chinese
Hamster Ovary Cells
N. T. Sam, R. Misaki, T. Ohashi, and K. Fujiyama
Arabidopsis thaliana 1,2-L-fucosyltransferase Catalyzes the Transfer of
Oligosaccharides
H. Ohashi, T. Ohashi, R. Misaki, and K. Fujiyama

L-galactose

to Xyloglucan

List of Publications: Kazuhito Fujiyama
Cake Layer Bacterial Communities during Different Biofouling Stages in Fullscale Membrane Bioreactors
K. Takada, T. Shiba, T. Yamaguchi, Y. Akane, Y. Nakayama, S. Soda, D. Inoue, and M. Ike
Colonization and Competition Dynamics of Plant Growth-Promoting/Inhibiting Bacteria in the Phytosphere of the
Duckweed Lemna minor
H. Ishizawa, M. Kuroda, K. Inoue, D. Inoue, M. Morikawa, and M. Ike
List of Publications: Michihiko Ike
Linfuranones B and C, Furanone-Containing Polyketides from a Plant-Associated Sphaerimonospora mesophila
H. Akiyama, C. Indananda, A. Thamchaipenet, A. Motojima, T. Oikawa, H. Komaki, A. Hosoyama, A. Kimura,N. Oku, and Y. Igarashi
Diversity of Nonribosomal Peptide Synthetase and Polyketide Synthase Gene Clusters among Taxonomically
Close Streptomyces Strains
H. Komaki, K. Sakurai, A. Hosoyama, A. Kimura, Y. Igarashi, and T. Tamura
List of Publications: Yasuhiro Igarashi
Dynamic Changes in Lysine Acetylation and Succinylation of the Elongation Factor Tu in Bacillus subtilis
S. Suzuki, N. Kondo, M. Yoshida, M. Nishiyama, and S. Kosono
Protein Acetylation on 2-isopropylmalate Synthase from Thermus Thermophilus HB27
A. Yoshida, M. Yoshida, T. Kuzuyama, M. Nishiyama, and S. Kosono
List of Publications: Saori Kosono
High-sensitive Liquid Chromatography-tandem Mass Spectrometry-based Chiral Metabolic Profiling Focusing
on Amino Acids and Related Metabolites
Y. Nakano, M. Taniguchi, and E. Fukusaki
Metabolomics Approach to Reduce the Crabtree Effect in Continuous Culture of Saccharomyces cerevisiae
M. Imura, R. Iwakiri, T. Bamba, and E. Fukusaki
List of Publications: Eiichiro Fukusaki
A 5' UTR-overlapping LncRNA Activates the Male-determining Gene Doublesex1 in the Crustacean Daphnia
magna
Y. Kato, C. A. G. Perez, N. S. M. Ishak, Q. D. Nong, Y. Sudo, T. Matsuura, T. Wada, and H. Watanabe
Microbiota Inoculum Composition Affects Holobiont Assembly and Host Growth in Daphnia
M. Callens, H. Watanabe, Y. Kato, J. Miura, and E. Decaestecker
List of Publications: Hajime WatanabeQuantitative Laser Diffraction for Quantification of Protein Aggregates: Comparison with Resonant Mass
Measurement, Nanoparticle Tracking Analysis, Flow Imaging, and Light Obscuration
S. Yoneda, B. Niederleitner, M. Wiggenhorn, H. Koga, S. Totoki, E. Krayukhina, W. Friess, and S. Uchiyama
Physicochemical Improvement of Rabbit Derived Single-domain Antibodies by Substitutions with Amino Acids
Conserved in Camelid Antibodies
N. Shinozaki, R. Hashimoto, M. Noda, and S. Uchiyama
List of Publications: Susumu Uchiyama
Anomalous Cell Migration Triggers a Switch to Deviation from the Undifferentiated State in Colonies of Human
Induced Pluripotent Stems on Feeder Layers
E. Shuzui, M. H. Kim, and M. Kino-oka
Role of Cell-secreted Extracellular Matrix Formation in Aggregate Formation and Stability of Human Induced
Pluripotent Stem Cells in Suspension Culture
M. H. Kim, K. Takeuchi, and M. Kino-oka
List of Publications: Masahiro Kino-oka
Analysis of Intracellular IgG Secretion in Chinese Hamster Ovary Cells to Improve IgG Production
K. Kaneyoshi, K. Uchiyama, M. Onitsuka, N. Yamano, Y. Koga, and T. Omasa
EGCG Improves Recombinant Protein Productivity in Chinese Hamster Ovary Cell Cultures Via Cell Proliferation
Control
N. Yamano, and T. Omasa
List of Publications: Takeshi Omasa
Expression of Engineered Carbonyl Reductase from Ogataea minuta in Rhodococcus opacus and its Application
to Whole-cell Bioconversion in Anhydrous Solvents
K. Honda, T. Ono, K. Okano, R. Miyake, Y. Dekishima, and H. Kawabata
Metabolic Engineering of Lactobacillus plantarum for Direct L-Lactic Acid Production from Raw Corn Starch
K. Okano, G. Uematsu, S. Hama, T. Tanaka, H. Noda, A. Kondo, and K. Honda
List of Publications: Kohsuke Honda
Butenolides from Streptomyces albus J1074 Act as External Signals to Stimulate Avermectin Production in
Streptomyces avermitilis
T. B. Nguyen, S. Kitani, S. Shimma, and T. Nihira
Discovery of a New Diol‑containing Polyketide by Heterologous Expression of a Silent Biosynthetic Gene Clusterfrom Streptomyces lavendulae FRI‑5
I. G. U. Pait, S. Kitani, F. W. Roslan, D. Ulanova, M. Arai, H. Ikeda, and T. Nihira
List of Publications: Shigeru Kitani

PUBLICATIONS by Visiting Professors and Research Scholar at Osaka University
Display of Escherichia coli Phytase on the Surface of Bacillus subtilis Spore Using CotG as an Anchor Protein
S. Mingmongkolchai, and W. Panbangred
Study on Sumbawa Gold Recovery Using Centrifuge
A. D. Ferdana, H. T. B. M. Petrus, I. M. Bendiyasa, I. D. Prijambada, F. Hamada, and T. Sachiko
Optimization of Gold Ore Sumbawa Separation Using Gravity Method: Shaking Table
A. D. Ferdana, H. T. B. M. Petrus, I. M. Bendiyasa, I. D. Prijambada, F. Hamada, and T. SachikoBacterial Diversity of Bat Guano from Cabalyorisa Cave, Mabini, Pangasinan, Philippines: A First Report on the
Metagenome of Philippine Bat Guano
M. P. D. Leon, A. D. Montecillo, D. S. Pinili, M. A. T. Siringan, and D.-S. Park
Near-Complete Genome Sequences of Streptomyces sp. Strains AC1-42T and AC1-42W, Isolated from Bat
Guano from Cabalyorisa Cave, Mabini, Pangasinan, Philippines
M. P. D. Leon, A-Y. Park, A. D. Montecillo, M. A. T. Siringan, A. R. R. Rosana, and S.-G. Kim

PUBLICATIONS by Collaborative Researchers
Potential Microbial Consortium Involved in the Biodegradation of Diesel, Hexadecane and Phenanthrene in
Mangrove Sediment Explored by Metagenomics Analysis.
P. Tiralerdpanich, P. Sonthiphand, E. Luepromchai, O. Pinyakong, and P. Pokethitiyook
Phenolic Compounds Removal by Grasses and Soil Bacteria after Land Application of Treated Palm Oil Mill
Effluent: A Pot Study.
P. Phonepaseuth, V. Rakkiatsakul, B. Kachenchart, O. Suttinun, and E. LuepromchaiUse of Agro-industrial Residue from the Canned Pineapple Industry for Polyhydroxybutyrate Production by
Cupriavidus Necator Strain A-04
V. Sukruansuwan, and S. C. Napathorn
Toward Non-toxic and Simple Recovery Process of Poly (3-hydroxybutyrate) Using the Green Solvent 1,3dioxolane
N. Yabueng, and S. C. Napathorn
Regulation of Periostin Expression by Notch Signaling in Hepatocytes and Liver Cancer Cell Lines
P. Kongkavitoon, P. Butta, A. Sanpavat, P. Bhattarakosol, P. Tangtanatakul, B. Wongprom, P. Tangkijvanich,N. Hirankarn, and T. Palaga
Recombinant Neuraminidase Pseudotyped Baculovirus: A Dual Vector for Delivery of Angiotensin II Peptides
and DNA Vaccine
I. Trianti, S. Akeprathumchai, P. Mekvichitsaeng, S. Rachdawong, and K. Poomputsa
Gene Duplication and Mutation in the Emergence of a Novel Aggressive Allele of the AVR-Pik Effector in the
Rice Blast Fungus
A. Longya, C. Chaipanya, M. Franceschetti, J. H. R. Maidment, M. J. Banfield, and C. Jantasuriyarat
Assessment of Genetic Variation of 15 Thai Elite Rice Cultivars Using InDel Makers
P. Moonsap, N. Laksanavilat, P. Tasanasuwan, S. Kate-Ngam, and C. Jantasuriyarat
List of Publications: Chatchawan Jantasuriyarat
Autophagy Related-protein 16-1 Up-regulated in Hepatitis B Virus-related Hepatocellular Carcinoma andImpaired Apoptosis
J. Peantum, A. Kunanopparat, N. Hirankarn, P. Tangkijvanich, and I. Kimkong
Enhancement of Ethanol Production in Very High Gravity Fermentation by Reducing Fermentation-induced
Oxidative Stress in Saccharomyces cerevisiae
T. Burphan, S. Tatip, T. Limcharoensuk, K. Kangboonruang, C. Boonchird, and C. Auesukaree
Phytohormone Priming Elevates the Accumulation of Defense-related Gene Transcripts and Enhances Bacterial
Blight Disease Resistance in Cassava
S. Yoodee, Y. Kobayashi, W. Songnuan, C. Boonchird, S. Thitamadee, I. Kobayashi, and J. Narangajavana
Utilization of Cellulose Microfiber from Pineapple Leaf as Lipase Immobilization Support for Highly Retained
Activity, Ease of Separation and Reusability
T. Pradubsang, P. Inprakhon, N. Patikarnmonthon, and T. Amornsakchai

Development of Amphiphilic Dextran-based Nanoparticle and in vitro Cytotoxicity in Human Colon
Adenocarcinoma cell
N. Patikarnmonthon, P. Inprakhon, and W. Panbangred
Proteomic and Immunomic Analysis of Schistosoma mekongi Egg Proteins
T. Thiangtrongjit, P. Adisakwattana, Y. Limpanont, P. Dekumyoy, S. Nuamtanong, P. Chusongsang,
Y. Chusongsang, and O. Reamtong
Immunome and Immune Complex-forming Components of Brugia malayi Identified by Microfilaremic Human
Sera
O. Reamtong, K. Rujimongkon, N. Sookrung, A. Saeung, T. Thiangtrongjit, Y. Sakolvaree, S. Thammapalo,
S. Loymek, and W. Chaicumpa
List of Publications: Onrapak Reamtong
Blood-induced Differential Gene Expression in Anopheles dirus Evaluated Using RNA Sequencing
M. Mongkol, W. Nguitragool, J. Sattabongkot, and A. Kubera
Lipid Production from a Mixture of Sugarcane Top Hydrolysate and Biodieselderived Crude Glycerol by the
Oleaginous Red Yeast, Rhodosporidiobolus fluvialis
R. Poontaweea, W. Yongmanitchai, and S. Limtong
Endophytic Yeasts Diversity in Leaf Tissue of Rice, Corn and Sugarcane Cultivated in Thailand Assessed by a
Culture-dependent Approach
P. Khunnamwong, S. Jindamorakot, and S. Limtong
List of Publications: Savitree Limtong
Rapid Screening Drug Susceptibility Test in Tuberculosis Using Sandwich Electrochemical Immunosensor
P. Chutichetpong, N. Cheeveewattanagul, P. Srilohasin, P. Rijiravanich, A. Chaiprasert, W. Surareungchai
Zwitterionic Peptide-capped Gold Nanoparticles for Colorimetric Detection of Ni2+
A. Parnsubsakul, S. Oaew, and W. Surareungchai
List of Publications: Waresak Surareungchai

PUBLICATIONS by Former Participants in
UNESCO International Post-Graduate University Course in Microbiology (UM),UNESCO Postgraduate Inter-University Course in Biotechnology (UB),and UNESCO Training Project supported by ODA Grants for UNESCO Activities, MEXT (UO)
Deletion of Pyruvate Decarboxylase Gene in Zymomonas mobilis by Recombineering through Bacteriophage
Lambda Red Genes
R. Khandelwal, S. Agrawal, D. Singhi, P. Srivastava, and V. S. Bisaria
Effectiveness of Cross-linked Enzyme Aggregates of Cellulolytic Enzymes in Hydrolyzing Wheat Straw
Shuddhodana, M. N. Gupta, and V. S. Bisaria
Biosynthetic Pathway and Optimal Conditions for the Production of Indole-3-acetic Acid by an Endophytic
Fungus, Colletotrichum fructicola CMU-A109
T. Numponsak, J. Kumla, N. Suwannarach, K. Matsui, and S. Lumyong
Morphology and Phylogeny of Atrocalyx acervatus sp. Nov. (Lophiotremataceae) from Acer Species
N. I. D. Silva, K. M. Thambugala, R. Jeewon, R. Phookamsak, I. D. Goonasekara, S. Lumyong, and K. D. Hyde
List of Publications: Saisamorn Lumyong
Effect of Adjunct Inoculation of Lactobacillus plantarum BS and Pediococcus acidilactici 3G3 on theMicrobiological, Physicochemical and Sensory Properties of Fermented Carabeef (pindang damulag)

V. L. Balmori, E. I. Dizon, A. S. A. Barrion, and F. B. Elegado
Identification and Characterization of Lactic Acid Bacteria Isolated from Some Medicinal and/or Edible
Philippine Plants
J. D. Saguibo, M. A. Mercado, S. T. Maldia, B. T. Jimeno, M. T. M. Perez, M. R. Calapardo, and F. B. Elegad
List of Publications: Francisco B. Elegado
Evaluation of Probiotic Bacillus aerius B81e Isolated from Healthy Hybrid Catfish on Growth, Disease
Resistance and Innate Immunity of Pla-mong Pangasius bocourti
R. Meidong, K. Khotchanalekha, S. Doolgindachbaporn, T. Nagasawa, M. Nakao, K. Sakai, and S. Tongpim
Amycolatopsis silviterrae sp. Nov., Isolated from Forest Soil
W. Jamjan, C. Suriyachadkun, S. Tanasupawat, K. Sakai, Y. Tashiro, Y. Okugawa, and S. Tongpim
Identification of Polyketide Synthase Gene Clusters in a Phage P1-derived Artificial Chromosome Library of aPhilippine Strain of Streptomyces sp. PCS3-D2
A. B. Custodio, and E. P. Alcantara
Rapid Detection of Listeria in Ice Cream in 13 Hours Using the Roka Listeria Detection Assay
S. Anderson, R. M. Cantera, L. M. Nadala, S.-yun Han, H. K. Janagama, J. M. Pando, B. Selover, S. L. Sung,
C. Nadala, and M. Samadpour
Application of an Environmental Phage-Based Assay (Sample6 Detect HT/L) for the Detection of Listeria spp.
in Ice Cream
E. V. Arias-Rios, K. Tenney, T. Mai, S. Anderson, Ruth Marie Cantera, Jasmine M. Pando, Brandon Selover,Lourdes M. Nadala, Seana K. Davidson, Mansour Samadpour
Hamatispora, a New Genus of Aquatic Fungi in Microthyriales Isolated from Fallen Leaves in Vietnam
L. T. H. Yen, K. Yamaguchi, Y. Tsurumi, D. V. Hop, and K. AndoRegulation of Nitrogen Fixation in Bradyrhizobium sp. Strain DOA9 Involves Two Distinct NifA Regulatory
Proteins That Are Functionally Redundant During Symbiosis but Not During Free-Living Growth
J. Wongdee, N. Boonkerd, N. Teaumroong, P. Tittabutr and E. Giraud
Mutualistic Co-evolution of T3SSs during the Establishment of Symbiotic Relationships between Vigna radiataand Bradyrhizobia
P. Piromyou, P. Songwattana, K. Teamtisong, P. Tittabutr, N. Boonkerd, P. A. Tantasawat, E. Giraud,
M. Göttfert, and N. Teaumroong
List of Publications: Neung Teaumroong
High Pullulan Biosynthesis from High Concentration of Glucose by a Hyperosmotic Resistant, Yeast-like Fungal
Strain Isolated from a Natural Comb-honey
Si-Jia Xue, Lu Chen, Hong Jiang, Guang-Lei Liu, Zhen-Ming Chi, Zhong Huc, Zhe Chia
An Insight into the Iron Acquisition and Homeostasis in Aureobasidium melanogenum HN6.2 Strain throughGenome Mining and Transcriptome Analysis
L. Yi, L. Guanglei, J. Hong, C. Zhenming, and C. Zhe
List of Publications: Chi Zhenming
Diversity of Endophytic Bacteria in Sweet Sorghum (Sorghum bicolor (L.) Moench) and their Potential for
Promoting Plant Growth
C. E.-de Fretes, R. Suryani, Y. A. Purwestri, T. R. Nuringtyas, and D. Widianto
Research Article Conjugated Linoleic Acid Synthesis in Milk Fermented with Lactobacillus casei Strain AG
Widodo, A. S. Sukarno, K. Estancia, D. Widianto, and Indratiningsih

Characterization of an Anaerobic, Thermophilic, Alkaliphilic, High Lignocellulosic Biomass-Degrading
Bacterial Community, ISHI-3, Isolated from Biocompost
A. Shikata, J. Sermsathanaswadi, T. Phakhinee, S. Baramee, C. Tachaapaikoon, R. Waeonukul, P. Pason,
K. Ratanakhanokchai, and A. Kosugi
Structural and Metabolic Adaptation of Cellulolytic Microcosm in Co-digested Napier Grass-swine Manure andits Application in Enhancing Thermophilic Biogas Production
S. Wongwilaiwalin, W. Mhuantong, V. Champreda, S. Tangphatsornruang, P. Panichnumsin,
K. Ratanakhanokchaie, and C. Tachaapaikoon
List of Publications: Khanok Ratanakhanokchai
Optimization of Succinic Acid Production by Succinic Acid Bacteria Isolated in Thailand
N. Pinkian, S. Phuengjayaem, S. Tanasupawat, and S. Teeradakorn
Optimization of Succinic Acid Production by Actinobacillus sp. NP9-aA7 using Plackett-Burman Design
Coupled with Box-Behnken Design
S. Phuengjayaem, S. Tanasupawat, and S. Teeradakorn
Novel Paranannizziopsis Species in a Wagler’s viper (Tropidolaemus wagleri), Tentacled Snakes (Erpeton
tentaculatum), and a Rhinoceros Snake (Rhynchophis boulengeri) in a Zoological Collection
K. L. Rainwater, N. P. Wiederhold, D. A. Sutton, M. M. Garner, C. Maguire, C. Sanders, C. F. Gibas,
J. F. Cano, J. Guarro, and A. M. Stchigel
From the Clinical Mycology Laboratory: New Species and Changes in Fungal Taxonomy and Nomenclature
N. P. Wiederhold, and C. F. Gibas
List of Publications: Connie F. Gibas
Regulation of Sensing, Transportation, and Catabolism of Nitrogen Sources in Saccharomyces cerevisiae
W. P. Zhang, G. C. Du, J. W. Zhou, and J. Chen
Modular Pathway Engineering of Key Carbon-precursor Supply-pathways for Improved N-acetylneuraminic
Acid Production in Bacillus subtilis
X. Zhang, Y. Liu, L. Liu, M. Wang, J. Li, G. Du, and J. Chen
List of Publications: Jian Chen
A Phosphofructokinase Homolog from Pyrobaculum calidifontis Displays Kinase Activity towards Pyrimidine
Nucleosides and Ribose 1-Phosphate
I. Aziz, T. Bibi, N. Rashid, R. Aono, H. Atomi, and M. Akhtar
List of Publications: Naeem Rashid
Bacillus Sp. Strain M10 as a Potential Biocontrol Agent Protecting Chili Pepper and Tomato Fruits from
Anthracnose Disease Caused by Colletotrichum capsici
P. Srikhong, K. Lertmongkonthum, R. Sowanpreecha, and P. Rerngsamran
Biocontrol of Orchid-pathogenic Mold, Phytophthora palmivora, by Antifungal Proteins from Pseudomonas
aeruginosa RS1
R. Sowanpreecha, and P. RerngsamranAdsorption of Iron (III) and Copper (II) by Bacterial Cellulose from Rhodococcus sp. MI 2
P. Yingkong, and S. Tanskul
A Medium Supplemented with Vegetable Extract Used for Cellulose Production of Rhodococcus SP. MI 2
S. Tanskul, T. Damthongsen, and N. Jaturonlak

The Presence of Multiple NS5A RASs is Associated with the Outcome of Sofosbuvir and Ledipasvir Therapy in
NS5A Inhibitor-naïve Patients with Chronic HCV Genotype 1b Infection in a Real-world Cohort
R. Kozuka, H. Hai, H. Motoyama, A. Hagihara, H. Fujii, S. Uchida-Kobayashi, H. Morikawa, M. Enomoto, Y.
Murakami, N. Kawada, and A. Tamori
Successful Direct-acting Antiviral Treatment of Three Patients with Genotype 2/1 Recombinant Hepatitis C
Virus
M. Okada, H. Hai, A. Tamori, S. Uchida‑Kobayashi, M. Enomoto, H. Kumada, and N. Kawada
A Simple Method for Direct Isolation of N-acyl-L-homoserine Lactone Mediated Biofilm-forming Rhizobacteria
from Roots
J. F. Begum, M. Tamilarasi, P. Pushpakanth, and D. Balachandar
Multiplex and Quantitative PCR Targeting SCAR Markers for Strain-level Detection and Quantification of
Biofertilizers
P. Reddypriya, A. Soumare, and D. Balachandar
List of Publications: Dananjeyan Balachandar
Effect of Two Alternative Methods of Pooling Sputum Prior to Testing for Tuberculosis with Genexpert
MTB/RIFN. T. B. Phuong, N. T. Anh, N. V. Son, V. Sintchenko, J. Ho, G. J. Fox, N. V. Nhung, and G. B. Marks
Prevalence of Latent Tuberculous Infection among Adults in the General Population of Ca Mau, Viet Nam
G. B. Marks, N. V. Nhun, T. A. Nguyen, N. B. Hoa, T. H. Khoa, N. V. Son, N. T. B. Phuong, D. M. Tin, J. Ho,and G. J. Fox
A Metabolomics-based Approach for the Evaluation of Off-tree Ripening Conditions and Different Postharvest
Treatments in Mangosteen (Garcinia mangostana)
A. P. Parijadi, S. Ridwani, F. Dwivany, S. P. Putri, and E. Fukusaki
Metabolic Repair through Emergence of New Pathways in Escherichia coli
S. Pontrelli, R. C. B. Fricke, S. T. Teoh, W. A. Laviña, S. P. Putri, S. Fitz-Gibbon, M. Chung, M. Pellegrini,
E. Fukusaki, and J. C. Liao
List of Publications: Sastia Prama Putri
Comprehensive Insights into Composition, Metabolic Potentials, and Interactions Among Archaeal, Bacterial,
and Viral Assemblages in Meromictic Lake Shunet in Siberia
Y.-T. Wu, C.-Y. Yang, P.-W. Chiang, C.-H. Tseng, H.-H. Chiu, I. Saeed, B. Baatar, D. Rogozin, S. Halgamuge,and A. Degermendzhi
Bacterial Community in Water and Air of Two Sub-Alpine Lakes in Taiwan
K. Tandon, S.-H. Yang, M.-T. Wan, B. Baatar, C.-C. Yang, C.-Y. Chiu, J.-W. Tsai, and S.-L. TangFirst Genome Sequence of Pasteurella multocida Type B Strain BAUTB2, a Major Pathogen Responsible for
Mortality of Bovines in Bangladesh
M. Shahjahan Ali Sarker, M. Tanvir Rahman, M. Muket Mahmud, M.-S. Tagliamonte, S.-M. Ziqrul Haq
Chowdhury, M. Rahfiqul Islam, M. Bahanur Rahman, M.-E. El Zowalaty, and K. H. M. Nazmul Hussain Nazir
GmWRKY49, a Salt Responsive Nuclear Protein, Improved Root Length and Govern Better Salinity Tolerancein Transgenic Arabidopsis
Z. Xu, Q. Raza, L. Xu, X. He, Y. Huang, J. Yi, D. Zhang, H. B. Shao, H. Ma, and Z. Ali
Inoculation with the Endophyte Piriformospora indica Significantly Affects Mechanisms Involved in Osmotic
Stress in Rice
M. A. B. Saddique, Z. Ali, A. S. Khan, I. A. Rana, and I. H. Shamsi
List of Publications: Zulfiqar Ali

The Purinergic Receptor P2X5 Contributes to Bone Loss in Experimental Periodontitis
H. Kim, T. Kajikawa, M. C. Walsh, N. Takegahara, Y. H. Jeong, G. Hajishengallis, and Y. Choi
Notch Signaling in Macrophages in the Context of Cancer Immunity
T. Palaga, W. Wongchana, and P. Kueanjinda
Brevibacillus sp. Promotes Maize Root Colonization by Acaulospora tuberculata and the Alteration of
Associated Plant Protein Responses
W. Yuttavanichakul, K. Teamtisong, N. Teaumroong, N. Boonkerd, and P. Tittabutr
List of Publications: Merkuria Karyantina
Molecular Identification and In vitro Propagation of Arbuscular Mycorrhiza from Tea Plant Rhizosphere
N. A. Arofatullah, S. Kabirun, K. Fujiyama, and D. Widianto
Optimization of Cultural Conditions for Improved Production of Intracellular Red Pigments by Monascus
purpureus using Response Surface Methodology
Ukan Sukandar Sastramihardja
Activities of International Center for Biotechnology for 2018
Author Index

ACTIVITIES OF ICBiotech

ACTIVITIES OF INTERNATIONAL CENTER FOR
BIOTECHNOLOGY FOR FY2018
The International Center for Biotechnology (ICBiotech) was founded in April 1995 as anindependent institute in Osaka University with a mission to pursue academic advancement
and collaborative research in biotechnology. ICBiotech has its origin from the “International
Center of Cooperative Research Center in Microbial Engineering Japan (ICME)” which wasestablished in the Faculty of Engineering, Osaka University in April 1978, through renaming
to “International Center of Cooperative Research in Biotechnology (ICBiotech)” in April
1985 with the recognition of the wide acceptance and success of ICME’s activities andachievements.
ICBiotech is dedicated to promote international cooperation among Asian countries in the
aspects of research and educational advancement in the field of Biotechnology by propelling
academic interactions in Asian countries, and is committed to industrial biotechnology studies
by means of microbial engineering and related sciences, focusing on the sustainableutilization of abundant natural resources in Southeast Asian countries.
ICBiotech serves as the seat of education and research in Asia, with the support of the
Ministry of Education, Culture, Sports, Science and Technology (Monbu-kagaku-sho,
MEXT), the Japan Society for the Promotion of Science (JSPS), the Japan Science andTechnology Agency (JST), the Japan Student Service Organization (JASSO), and theauspices of the United Nations Educational, Scientific and Cultural Organization (UNESCO),
with the cooperation of Department of Biotechnology, Graduate School of Engineering,Osaka University, and researchers from prestigious universities nationwide and abroad.
In 2002 Mahidol University-Osaka University Collaborative Research Center for Bioscience and Biotechnology: MU-OU:CRC was set up at Faculty of Science, Mahidol
University as a collaborative research center to accomplish multidisciplinary research in thefield of Bioscience and Biotechnology.
The activities of the ICBiotech include:
1. Research and Education
The main area of research is industrial biotechnology rooted in microbial engineering,whilst centering on the sustainable use of agricultural and forest resources in bioresourcerich countries such as those in Southeast Asia. Research is underway in the field of cellengineering with the objectives of analyzing the cellular functions of bacteria, fungi andplants, and developing and using functions of these cells for management and rational useof biological resources that exist on our planet. ICBiotech covers three areas of Biotechnology:
1) Discovery of new functions from biological resources.
2) Bio-conversion and process engineering of biological resources.
3) Conservation of biological resources.
For under graduate and post graduate courses, ICBiotech is involved in the education
activities of Department of Biotechnology, Graduate School of Engineering as collaborating laboratories and currently covers several fields of biotechnology in education andresearch: Molecular Microbiology laboratory chaired by Prof. Takuya Nihira and Applied
Microbiology laboratory chaired by Prof. Kazuhito Fujiyama.

2. Participating in FrontierLab@OsakaU ’Scientific Empowerment Program for International Students’ which was created for international students to conduct thematicstudies and achieve results under the guidance of supervisors while acquiring skills
necessary for continuing research in one of Osaka University’s internationally renowned
science and technology laboratories for a period of up to 12 months.
3. Acting as collaborating laboratories with Department of Biotechnology, Graduate Schoolof Engineering, Osaka University for ‘Biotechnology Global Human Resource
Development program’, a five-year program for master’s and doctor’s degrees. The aim of
this program is to expose graduate students (privately financed as well as the Japanese
Government Scholarship students) to state-of-the-art research skills and in-depth
knowledge of advanced biology, chemistry and physics to harness the potential of
biotechnology.
4. Having been administrating and implementing the “UNESCO Biotechnology School inAsia” (2012-2016) at Department of Biotechnology, Graduate School of Engineering,Osaka University, taking over the long-termed “International Post-Graduate University
Course in Microbiology” (1972-2003) and Postgraduate Inter-University Course in
Biotechnology (2004-2007) with total of 459 alumni spreading all over Asia.
5. Serving as the secretariat for the UNESCO Regional Network for Microbiology andBiotechnology in Southeast Asia in accordance with a proposed memorandum resulting
from a Consortium Meeting of the Project “Tropical Bio-Resources and Green Chemistry
Strategy” (Osaka 2003).
6. Working as a member of the global network “Microbiological Resource Centers”(MIRCENs) operated by UNEP, UNESCO and ICRO; and as an original member of the“Regional Network for Microbiology in Southeast Asia” operated under the auspices of
UNESCO through the Fund-In-Trust of the Japanese Government (J-FIT).
7.

Promoting international cooperative researches in biotechnology with the Southeast
Asian countries related to Biotechnology, under the support of Monbu-kagaku-sho
Grant-in-Aids for International Scientific Research. In addition, ICBiotech cooperates indeveloping international organization and conducting academic seminars related tobiotechnology.

8.

Publishing Annual Reports of ICBiotech periodically.

STEERING COMMITIEE
ChairmanCommittee Members

Prof. Kazuhito Fujiyama (Director of ICBiotech)
Prof. Eiichiro Fukusaki (Graduate School of Engineering)
Prof. Tsuyoshi Inoue (Graduate School of Engineering)
Prof. Hajime Watanabe (Graduate School of Engineering)
Prof. Kazumasa Hirata (Graduate School of Pharmaceutical Sciences)Prof. Masahito Taya (Graduate School of Engineering Science)Prof. Toshihiro Horii (Research Institute for Microbial Diseases)

STAFF
Director/Professor
Adjunct Professor

Dr. Kazuhito Fujiyama
Dr. Michihiko Ike(Graduate School of Engineering, Osaka University)

Collaborative Professor

Dr. Watanalai Panbangred (Mahidol University, Thailand)
Dr. Irfan Dwidya Prijambada(Universitas Gadjah Mada, Indonesia)

Dr. Raymond L. Rodriguez
Guest Professor

Adjunct Associate Professor
Guest Associate Professor
Visiting Academic Staff

Associate Professor
Assistant Professor
Administrative Official
Administrative AssistantTechnical Assistant(University of California-Davis, USA)
Dr. Toshiomi Yoshida (Prof. Emer., Osaka University)
Dr. Tatsuji Seki (Prof. Emer., Osaka University)
Mr. Junji Tayama (Osaka University)
Dr. Yasuhiro Igarashi (Toyama Prefectural University)

Dr. Yoshinori Sumimura(Center for Global Initiatives, Osaka University)
Dr. Saori Kosono (The University of Tokyo)
Dr. Vu Thu Trang (Hanoi University of Science and Technology,
Vietnum) (Specially Appointed Associate Professor
under the Cross-appointment Agreement)
Dr. Nantana Srisuk (Kasetsart University, Thailand) (Specially
Appointed Associate Professor)
Dr. Marian Pulido De Leon (University of the Philippines Los
Banos, Philippines) (Specially Appointed Associate Professor
under the Cross-appointment Agreement)
Dr. Jigjiddorj Enkh-Amgalan (Institute of General andExperimental Biology, Mongolian Academy of Sciences,
Mongolia) (Specially Appointed Associate Professor under the
Cross-appointment Agreement)

Dr. Shigeru Kitani
Dr. Ryo Misaki
Dr. Hiroshi Kinoshita
Dr. Takao Ohashi
Ms. Reiko Matsumoto
Ms. Fumiko Tomomatsu
Ms. Megumi Okamoto
Ms. Chiyoko Katayama
Ms. Shizuka Kawakami
Ms. Kyoko Sugimoto
Ms. Terumi Kikuchi

I. COOPERATIVE RESEARCH STATION (CRS) IN SOUTHEAST ASIA
The ICBiotech, Osaka University launched out the Cooperative Research Station (CRS) in
Southeast Asia at Chalermprakiat Building, Faculty of Science, Mahidol University in 2002
through the generous support by Mahidol University. The CRS's space and equipments are
made available for Southeast Asian and Japanese researchers to undertake cooperative onsite
researches on the development of the abundant natural biological and genetic resources andtheir sustainable utilization in Southeast Asian countries through JSPS core university
program (ended in 2005), JST Special Coordination Funds for Promoting Science andTechnology (2006-2009) and JSPS Asian CORE Program (2009-2014). The CRS alsofunctioned as a lecturing and research station of the UNESCO International Post-graduate
Inter-University program, which Osaka University operated in coordination with Thai
universities. The CRS is considering support to the alumni of Osaka University and provisionof university information for recruitment of students for study in Osaka University.
Mahidol University (MU) and Osaka University (OU) together established the Mahidol
University-Osaka University Collaborative Research Center (MU-OU:CRC) for Bioscienceand Biotechnology at Faculty of Science, Mahidol University in 2002, to strengthen the
research cooperation in these fields which are amongst the most active fields of study andresearch in both universities.
Currently, MU-OU:CRC has coordinated a research projects under the jointly support ofNational Research Council of Thailand (NRCT), National Center for Genetic Engineering andBiotechnology (BIOTEC) and The Japan Society for the Promotion of Science (JSPS).
Researchers from Mahidol University, Chulalongkorn University, Kasetsart University, King
Mongkut's University of Technology Thonburi and BIOTEC participate in this project.
CRS is conducting researches on
1. Strain improvement of Bacillus thuringiensis.
2. Screening of actinomycetes for bioactive compound.
3. The soil metagenome for screening of potential polysaccharide-degrading enzymes.
4. Cell culture system for screening of bioactive compounds from Streptomycetes andenvironmental fungi.
5. Quorum sensing interference: Novel biocontrol strategies for pathogenic bacteria.

II. WORKSHOPThe 8th Workshop on Collaboration
between Osaka University and Japanese companies
for global human resource development in the field of biotechnology

February 1, 2019
International Center for Biotechnology, Osaka University
Sponsorship; International Center for Biotechnology, Osaka University
Co-sponsored; International Research Initiative for Frontier of Industrial Biotechnology, Osaka University

The eighth Workshop on Collaboration between Osaka University and Japanesecompanies was held. Representatives of industries engaged in the biotechnology industry
were introduced to the international students enrolled at Osaka University for the purpose of
attaining international human resource development. A total of ten enthusiastic companies
attended the meeting. Companies, faculty members and students introduced themselves andtheir activities to one another. Students were able to know about the Japanese companies andhow to apply their scholarly works in the industrial biotechnology setting. Adding to the great
success of this meeting are the lively discussions and exchange meetings which have resulted
in a deepened understanding of each other.
13:45: Opening remark by Prof. Kazuhito Fujiyama
13:50-14:30: Special lecture “The Way for Science Graduates to Survive in a Company”
by Katsuyuki Kawatsura (Asahi Holdings, Inc.)
14:30-15:00: Coffee Break
15:00-15:20: Keynote Address by Prof. Kazuhito Fujiyama
15:20-17:30: Group talking with individual company(45 min each, three rounds at α building and β building in ICBiotech)
15:30-16:05: 1st Round
16:10-16:45: 2nd Round
16:50-17:25: 3rd Round
Attending companies are:Earth Environmental Service Co. (α-508)
Asahi Group Holdings, Ltd. (α-506)
Ajinomoto General Foods, Inc. (α-502)
Kao (α-401)
Glico Nutrition Co. (α-510)
Saraya Co. (α-509)
Shionogi Administration Service Co. (α-511)
Sysmex Corp. (α-504)
Shimadzu Corp. (β-303)
Lion Corp. (α-504)
17:45-19:30: Simple party with light meal at International saloon (β-102).

III. JASSO STUDENT EXCHANGE SUPPORT PROGRAM
Scholarship for Short Stay/ Short Visit Program (SSSV)This is a field study program jointly operated with several universities in Thailand. In
2018, 27 first year students of the master's course of Osaka University visited 4 universities inThailand between August 5 and September 10, and 16 postgraduate students from Thailand
and the Philippines visited Osaka University between November 2018 and March 2019,
which enhanced mutual interactions.
IV. JST Japan-Asia Youth Exchange Program in Science(SAKURA Exchange Program in Science)
Purpose of the Program: Promoting science and technology is a key engine to materializea bright future of Asia and it is vitally important to enhance the exchange of youths in Asian
countries and Japan who will play a crucial role in the field of science and technology. Basedon this concept, “Japan-Asia Youth Exchange Program in Science” (SAKURA Exchange
Program in Science) is the program for enhancing exchanges between Asia and Japan of the
youths who will play a crucial role in the future field of science and technology through theclose collaboration of industry-academia-government by facilitating short-term visits ofcompetent Asian youths to Japan. This program aims at raising the interest of Asian youths
toward the leading Japanese science and technologies at Japanese universities, research
institutions and private companies.
Schedule
January 20
January 21
January 22
January 23

January 24
January 25
January 26
January 27

Arriving
Orientation, Seminar on advanced biotechnology I
Welcome Party
Seminar on advanced biotechnology II, Cutting-edge biotechnology experiment I
Lab Visit in OU Shimadzu Analytical Innovation Research Laboratory
Cutting-edge biotechnology experiment II
Lab Visit in Department of Biotechnology and Analytical Instrumentation Facility, OU
and Hitz
Exchange party with OU students
Seminar on advanced biotechnology III
Visit to Hakutsuru Sake Brewery Museum and Daikin Solution Plaza fuha
Seminar on advanced biotechnology IV
Visit to Shimadzu Company in Kyoto and Kinkakuji-Temple
Presentation of task, Certificate award
Closing Ceremony
Back home

V. SCIENTIST EXCHANGES
Record of Scientist Exchange (FY2018)
From Japan to counterpart countries / Between counterpart countries*Please contact us for more information.

VI. GUESTS/VISITORS*Please contact us for more information.

VII. STEERING COMMITTEE MEETING 2018
Steering Committee Meetings of ICBiotech were convened as follows:・October 16, 2018: Discussion on ICBiotech Director`s supplementary assignment to Director of
ASEAN Center:
Dr. Kazuhito Fujiyama (October 16, 2018 – March 31, 2019)
・November 19, 2018: Discussion on the conclusion of the academic exchange agreement between
College of Biological Sciences and College of Engineering, University ofCalifornia, Davis and International Center for Biotechnology, Osaka University
・November 22, 2018: Discussion on the conclusion of the cross appointment agreement:
Dr. Enkh-Amgalan Jigjiddorj (March 1 – 29, 2019)
・December 6, 2018

Report on:
1. JST Sakura Exchange Program in Science
2. JASSO Short-stay and Short-visit program 2018
(Aug. 5, 2018 – Sept. 11, 2018 sending Japanese students to Thailand,
Nov. 5, 2018 to Feb. 10, 2019 accepting Thai students)
3. Four Visiting Academic Staff
Dr. Vu Thu Trang from Vietnam, April 16 – May 15, 2018
Dr. Srisuk Nantana from Thailand, May 15 – June 13, 2018
Dr. Marian Pulido De Leon from Philippine, June 1 – 29, 2018
Dr. Enkh-Amgalan Jigjiddorj from Mongol, March 1 – 29, 2019

4. Adjunct Professors for the FY2019
5. Other topics: Organization of ICBiotech
Discussion on:
1. Selection of Director of ICBiotech:
Dr. Kazuhito Fujiyama (April 1, 2019 – March 31, 2021)
2. Selection of members of Steering Committee:
Dr. Mamoru Tobisu (April 1, 2019 – March 31, 2021)
Dr. Hiroshi Umakoshi (April 1, 2019 – March 31, 2021)
Dr. Genji Kurisu (April 1, 2019 – March 31, 2021)

・February 1, 2019:
Discussion on:
1. Selection of a member of Steering Committee:
Dr. Tetsuya Iida (April 1, 2019 – March 31, 2021)
2. Inviting a Visiting Research Scholar:
Mr. Tuan Hoang Viet (April 1 – 30, 2019)・March 1, 2019:
Discussion on:*Retirement and open recruitment of an Assistant Professor*Selection of a member of Evaluation Committee:
Dr. Hiroshi Umakoshi (April 1, 2019 – March 31, 2020)・March 12, 2019: Discussion on accepting a Visiting Researcher:Dr. Takao Ohashi (April 1, 2019 – March 31, 2020)・March 19, 2019: Discussion on concluding the cross appointment agreement:
Dr. Napathorn, Suchada Chanprateep (January 6 – January 31, 2020)