ANNUAL REPORTS
OF
INTERNATIONAL CENTER FOR BIOTECHNOLOGYOSAKA UNIVERSITY
VOL. 37, 2015

DIRECTOR/EDITORTAKUYA NIHIRAASSISTANT EDITOR

KAZUHITO FUJIYAMASHIGERU KITANIRYO MISAKIHIROSHI KINOSHITATAKAO OHASHISECRETARY

MUTSUKO TSUJI
FUMIKO TOMOMATSUCHIYOKO KATAYAMAHITOMI YUMIOKASHIZUKA KAWAKAMI

The Annual Report is published to record the activity of theInternational Center for Biotechnology (ICBiotech) and issued once in eachfiscal year. It contains scientific articles, progress reports, letters, andannouncement from the Center. This volume includes publications by theformer participants in UNESCO courses. The editor welcomes thesubmission of appropriate articles from all persons who are concerned withthe activity of the Center. All the contributions, however, will be
reviewed by editors before their acceptance. The scientific paper hereinshould be treated as personal communications and not treated as original
publications. The Annual Report is distributed upon request to theInternational Center for Biotechnology, Osaka University, 2-1 Yamadaoka,
Suita, Osaka 565-0871, Japan (e-mail: info_icbio@icb.osaka-u.ac.jp).

1

CONTENTS
Characterization of the Biosynthetic Gene Cluster for Maklamicin, a Spirotetronate-class Antibiotic of theEndophytic Micromonospora sp. NBRC 110955
R. Daduang, S. Kitani, J. Hashimoto, A. Thamchaipenet, Y. Igarashi, K. Shin-ya, H. Ikeda, and T. Nihira

129-Deoxymaklamicin, a New Maklamicin Analogue Produced by a Genetically Engineered Strain of
Micromonospora sp. NBRC 110955
R. Daduang, S. Kitani, Y. Sudoh, I. G. U. Pait, A. Thamchaipenet, H. Ikeda, Y. Igarashi, and T. Nihira

11

List of Publication: Takuya Nihira

11

Substrate Preference of Citrus Naringenin Rhamnosyltransferases and Their Application to Flavonoid
Glycoside Production in Fission Yeast
T. Ohashi, Y. Hasegawa, R. Misaki, and K. Fujiyama

12

The Combination of Plant Translational Enhancers and Terminator Increase the Expression of Human
Glucocerebrosidase in Nicotiana benthamiana Plants
J. Limkul, R. Misaki, K. Kato, and K. Fujiyama

22

List of Publication: Kazuhito Fujiyama

23

Pilot Test of Biological Removal of 1,4-dioxane from a Chemical Factory Wastewater by Gel Carrier
Entrapping Afipia sp. Strain D1
K. Isaka, M. Udagawa, K. Sei, and M. Ike

24

Effects of the C/N Ratio and Bacterial Populations on Nitrogen Removal in the Simultaneous Anammox
and Heterotrophic Denitrification Process: Mathematic Modeling and Batch Experiments
Z. Bi, M. Takekawa, G. Park, S. Soda, S. Qiao, and M. Ike

32

List of Publication: Michihiko Ike

33

Extra-facile Chiral Separation of Amino Acid Enantiomers by LC-TOFMS Analysis
Y. Konya, T. Bamba, and E. Fukusaki

34

A Metabolomics-based Strategy for Identification of Gene Targets for Phenotype Improvement and Its
Application to 1-Butanol Tolerance in Saccharomyces cerevisiae
S. T. Teoh, S. Putri, Y. Mukai, T. Bamba, and E. Fukusaki

39

List of Publication: Eiichiro Fukusaki

40

A Fast- and Positively Photoswitchable Fluorescent Protein for Ultralow-laser-power RESOLFT
Nanoscopy
D. K. Tiwari, Y. Arai, M. Yamanaka, T. Matsuda, M. Agetsuma, M. Nakano, K. Fujita, and T. Nagai

41

Expanded Palette of Nano-lantern for Real-time Muliticolor Luminescence Imaging
A. Takai, M. Nakano, K. Saito, R. Haruno, T. M. Watanabe, T. Ohyanagi, T. Jin, Y. Okada, and T. Nagai

73

List of Publication: Tekeharu Nagai

74

Characterization of a Gamma-butyrolactone Synthetase Gene Homologue (stcA) Involved in Bafilomycin
Production and Aerial Mycelium Formation in Streptomyces sp. SBI034
B. Intra, J. Euanorasetr, T. Nihira, and W. Panbangred

75

Kinetic Properties and Stability of Glucose Dehydrogenase from Bacillus amyloliquefaciens SB5 and Its
Potential for Cofactor Regeneration
T. Pongtharangkul, P. Chuekitkumchorn, N. Suwanampa, P. Payongsri, K. Honda, and W. Panbangred

87

List of Publication: Watanalai Panbangred

88iExpression of Recombinant T-cell Epitopes of Major Japanese Cedar Pollen Allergens Fused with
Cholera Toxin B Subunit in Escherichia coli
V. V. Hoang, Y. Zou, K. Kurata, and K. Enomoto

89

Characterization of Oleaginous Yeasts Accumulating High Levels of Lipid when Cultivated in Glycerol
and Their Potential for Lipid Production from Biodiesel-derived Crude Glycerol
P. Poulburee, W. Yongmanitchai, N. Lertwattanasakul, T. Ohashi, K. Fujiyama, and S. Limtong

97

The diversity of Culturable Yeasts in the Phylloplane of Rice in Thailand
S. Limtong and R. Kaewwichian108

List of Publication: Savitree Limtong

109

PUBLICATIONS by Former Participants in
UNESCO International Post-Graduate University Course in Microbiology (UM),UNESCO Postgraduate Inter-University Course in Biotechnology (UB),and UNESCO Training Project supported by ODA Grants for UNESCO Activities, MEXT (UO)
Effect of Agricultural Amendments on Cajanus cajan (Pigeon Pea) and Its Rhizospheric Microbial
Communities – A Comparison between Chemical Fertilizers and Bioinoculants
R. Gupta, V. S. Bisaria, and S. Sharma

111

Piriformospora indica Enhances the Production of Pentacyclic Triterpenoids in Lantana camara L.
Suspension Cultures
P. Kumar, R. Chaturvedi, D. Sundar, and V. S. Bisaria

128

List of Publication: Virendra S. Bisaria

128

Functional Golden Brown Rice Fermentation by Monascus sp. Yellow, White Parental Strains and Their
Protoplast Mutants and FusantsP. Thongpradis, S. Trakulnaleamsai, C. Pothiratana, and B. Yongsmith

129

Viable Counts of Lactic Acid Bacteria in Philippine Commercial Yogurts
R. B. Guevarra and V. L. Barraquio

142

Human Exposure Risk Assessment to Biogenic Amines in Cheeses and Fermented Sausages in Southern
Luzon
B. P. Cadayong and V. L. Barraquio

147

Functional Analysis of Genes Involved in the Biosynthesis of Enterocin NKR-5-3B, a Novel CircularBacteriocin
R. H. Perez, N. Ishibashi, T. Inoue, K. Himeno, Y. Masuda, N. Sawa, T. Zendo, P. Wilaipun,
V. Leelawatcharamas, J. Nakayama, and K. Sonomoto

148

Novel Toxicity of Bacillus thuringiensis Strains against the Melon Fruit Fly, Bactrocera cucurbitae(Diptera: Tephritidae)
M. A. Shishir, A. Akter, M. Bodiuzzaman, M. A. Hossain, M. M. Alam, S. A. Khan, S. N. Khan,and M. M. Hoq

158

Efficacy of Indigenous Bacillus thuringiensis Strains for Controlling Major Vegetable Pests in
Bangladesh
A. Shishir, A. A. Bhowmik, N. R. Akanda, A. A. Mamun, S. N. Khan, and M. M. Hoq

167

List of Publication: Md. Mozammel Hoq

168

Yeast Surface Display of Two Proteins Previously Shown to Be Protective Against White Spot Syndrome
Virus (WSSV) in Shrimp
169
V. Ananphongmanee, J. Srisala, K. Sritunyalucksana, and C. Boonchirdii

Traditional Dried Starter Culture (Medombae) for Rice Liquor Production in Cambodia
C. Chim, I. D. Erlinda, F. B. Elegado, A. W. Hurtada, N. Chakrya, and C. L. Raymundo

183

Bacteriocins from Lactic Acid Bacteria: A Review of Biosynthesis, Mode of Action, Fermentative
Production, Uses, and Prospects
R. H. Perez, M. T. M. Perez, and F. B. Elegado

192

List of Publication: Francisco B. Elegado

192

Rhizobium–Legume Symbiosis in the Absence of Nod Factors: Two Possible Scenarios with or without
the T3SS
S. Okazaki, P. Tittabutr, A. Teulet, J. Thouin, J. Fardoux, C. Chaintreuil, D. Gully, J.-F. Arrighi,
N. Furuta, H. Miwa, M. Yasuda, N. Nouwen, N. Teaumroong, and E. Giraud

193

The Type III Secretion System (T3SS) Is a Determinant for Rice-Endophyte Colonization by
Non-Photosynthetic Bradyrhizobium
P. Piromyou, P. Songwattana, T. Greetatorn, T. Okubo, K. Chiba Kakizaki, J. Prakamhang,
P. Tittabutr, N. Boonkerd, N. Teaumroong, and K. Minamisawa

204

List of Publication: Neung Teaumroong

205

The Recovery and Bioproperties of a Xylanolytic Multi-enzyme Complex from Tepidimicrobium
xylanilyticum BT14
P. Phitsuwan and K. Ratanakhanokchai

206

Alkaline Xylanolytic–Cellulolytic Multienzyme Complex from the Novel Anaerobic
Alkalithermophilic Bacterium Cellulosibacter alkalithermophilus and Its Hydrolysis of Insoluble
Polysaccharides under Neutral and Alkaline Conditions
S. Baramee, P. Phitsuwan, R. Waeonukul, P. Pason, C. Tachaapaikoon, A. Kosugi, andK. Ratanakhanokchai

216

List of Publication: Khanok Ratanakhanokchai

217

Creation of Hansenula polymorpha Fatty Acid Auxotrophic Mutants for Genetic Study of Fatty Acid
Biosynthesis
S. Sooksai, K. Laoteng, P. Chewchanlertfa, and S. Noitang

218

Compartmentalizing Metabolic Pathway in Candida glabrata for Acetoin Production
S. Li, L. Liu, and J. Chen

224

Metabolic Engineering in the Biotechnological Production of Organic Acids in the Tricarboxylic Acid
Cycle of Microorganisms: Advances and Prospects
X. Yin, J. H. Li, H. D. Shin, G. C. Du, L. Liu, and J. Chen

231

List of Publication: Jian Chen232

Use of Bacterial Surrogates as a Tool to Explore Antimalarial Druginteraction: Synergism between
Inhibitors of Malarial Dihydrofolate Reductase and Dihydropteroate Synthase
Y. Talawanich, S. Kamchonwongpaisan, W. Sirawaraporn, and Y. Yuthavong

233

Medium Chain Length Polyhydroxyalkanoates Consisting Primarily of Unsaturated
3-Hydroxy-5-Cis-Dodecanoate Synthesized by Newly Isolated Bacteria Using Crude Glycerol
A. Muangwong, T. Boontip, J. Pachimsawat, and S. C. Napathorn

239

Optimisation of the Use of Products from the Cane Sugar Industry for Poly(3-Hydroxybutyrate)
Production by Azohydromonas lata DSM 1123 in Fed-batch Cultivation
N. Wisuthiphaet and S. C. Napathorn

256

iii

Effect of Bacillus subtilis CGMCC 1.1086 on the Growth Performance and Intestinal Microbiota ofBroilers
Y. Li, Q. Xu, Z. Huang, L. Lv, X. Liu, C. Yin, H. Yan, and J. Yuan

257

Pathway for Biodegrading Microcystin-YR by Sphingopyxis sp. USTB-05
H. Xu, H. Wang, Q. Xu, L. Lv, C. Yin, X. Liu, H. Du, and H. Yan

267

Randomized Trial of Combined Triple Therapy Comprising Two Types of Peginterferon with Simeprevir
in Patients with Hepatitis C Virus Genotype 1b
A. Tamori, K. Yoshida, O. Kurai, K. Kioka, H. Hai, R. Kozuka, H. Motoyama, E. Kawamura,
268
A. Hagihara, S. Uchida-Kobayashi, H. Moririkawa, M. Enomoto, Y. Murakami, and N. Kawada
Cytoglobin Deficiency Promotes Liver Cancer Development from Hepatosteatosis through Activation of
the Oxidative Stress Pathway
L. T. T. Thuy, Y. Matsumoto, T. T. Thuy, H. Hai, M. Suoh, Y. Urahara, H. Motoyama, H. Fujii,
A. Tamori, S. Kubo, S. Takemura, T. Morita, K. Yoshizato, and N. Kawada

278

List of Publication: Hoang Hai

278

Development of Pgrac100-based Expression Vectors Allowing High Protein Production Levels in
Bacillus subtilis and Relatively Low Basal Expression in Escherichia coli
T. T. P. Phan, L. T. Tran, W. Schumann, and H. D. Nguyen

279

A Novel Method to Predict Highly Expressed Genes Based on Radius Clustering and Relative
Synonymous Codon Usage
T. A. Tran, N. T. Vo, H. D. Nguyen, and B. T. Pham

288

The Nuclear Protein GmbZIP110 Has Transcription Activation Activity and Plays Important Roles in the
Response to Salinity Stress in Soybean
Z. Xu, Z. Ali, L. Xu, X. He, Y. Huang, J. Yi, H. Shao, H. Ma, and D. Zhang

289

Alterations of DNA Methylation in Diverse Grafred Hybrid Tomatoes (Solanum lycopersicum L.)
X. Xincheng, Z. Ali, S. Weiyi, N. Ghori, S. Hongbo, and D. Jinping

301

List of Publication: Zulfiqar Ali

301

PD-1 Upregulated on Regulatory T Cells during Chronic Virus Infection Enhances the Suppression of
CD8+ T Cell Immune Response via the Interaction with PD-L1 Expressed on CD8+ T Cells
H. J. Park, J. S. Park, Y. H. Jeong, J. Son, Y. H. Ban, B.-H. Lee, L. Chen, J. Chang, D. H. Chung,I. Choi, and S.-J. Ha

302

Activities of International Center for Biotechnology for 2015315

Author Index

330

iv

ACTIVITIES OF ICBiotech

315

ACTIVITIES OF INTERNATIONAL CENTER FOR
BIOTECHNOLOGY FOR FY2015
The International Center for Biotechnology (ICBiotech) was founded in April 1995 as anindependent institute in Osaka University with a mission to pursue academic advancement
and collaborative research in biotechnology. ICBiotech has its origin from the “International
Center of Cooperative Research Center in Microbial Engineering Japan (ICME)” which wasestablished in the Faculty of Engineering, Osaka University in April 1978, through renaming
to “International Center of Cooperative Research in Biotechnology (ICBiotech)” in April
1985 with the recognition of the wide acceptance and success of ICME’s activities andachievements.
ICBiotech is dedicated to promote international cooperation among Asian countries in the
aspects of research and educational advancement in the field of Biotechnology by propelling
academic interactions in Asian countries, and is committed to industrial biotechnology studies
by means of microbial engineering and related sciences, focusing on the sustainableutilization of abundant natural resources in Southeast Asian countries.
ICBiotech serves as the seat of education and research in Asia, with the support of the
Ministry of Education, Culture, Sports, Science and Technology (Monbu-kagaku-sho,
MEXT), the Japan Society for the Promotion of Science (JSPS), the Japan Science andTechnology Agency (JST), the Japan Student Service Organization (JASSO), and theauspices of the United Nations Educational, Scientific and Cultural Organization (UNESCO),
with the cooperation of Department of Biotechnology, Graduate School of Engineering,Osaka University, and researchers from prestigious universities nationwide and abroad.
In 2002 Mahidol University-Osaka University Collaborative Research Center for Bioscience and Biotechnology: MU-OU:CRC was set up at Faculty of Science, Mahidol
University as a collaborative research center to accomplish multidisciplinary research in thefield of Bioscience and Biotechnology.
The activities of the ICBiotech include:
1. Research and Education
The main area of research is industrial biotechnology rooted in microbial engineering,whilst centering on the sustainable use of agricultural and forest resources in bioresourcerich countries such as those in Southeast Asia. Research is underway in the field of cellengineering with the objectives of analyzing the cellular functions of bacteria, fungi andplants, and developing and using functions of these cells for management and rational useof biological resources that exist on our planet. ICBiotech covers three areas of Biotechnology:
1) Discovery of new functions from biological resources.
2) Bio-conversion and process engineering of biological resources.
3) Conservation of biological resources.
For under graduate and post graduate courses, ICBiotech is involved in the education
activities of Department of Biotechnology, Graduate School of Engineering as collaborating laboratories and currently covers several fields of biotechnology in education andresearch: Molecular Microbiology laboratory chaired by Prof. Takuya Nihira and Applied
Microbiology laboratory chaired by Prof. Kazuhito Fujiyama.

316

2. Participating in FrontierLab@OsakaU ’Scientific Empowerment Program for International Students’ which was created for international students to conduct thematicstudies and achieve results under the guidance of supervisors while acquiring skills
necessary for continuing research in one of Osaka University’s internationally renowned
science and technology laboratories for a period of up to 12 months.
3. Acting as collaborating laboratories with Department of Biotechnology, Graduate Schoolof Engineering, Osaka University for ‘Biotechnology Global Human Resource
Development program’, a five-year program for master’s and doctor’s degrees. The aim of
this program is to expose graduate students (privately financed as well as the Japanese
Government Scholarship students) to state-of-the-art research skills and in-depth
knowledge of advanced biology, chemistry and physics to harness the potential of
biotechnology.
4. Having been administrating and implementing the “UNESCO Biotechnology School inAsia” (2012-) at Department of Biotechnology, Graduate School of Engineering, Osaka
University, taking over the long-termed “International Post-Graduate University Course in
Microbiology” (1972-2003) and Postgraduate Inter-University Course in Biotechnology(2004-2007) with total of 459 alumni spreading all over Asia.
5. Serving as the secretariat for the UNESCO Regional Network for Microbiology andBiotechnology in Southeast Asia in accordance with a proposed memorandum resulting
from a Consortium Meeting of the Project “Tropical Bio-Resources and Green Chemistry
Strategy” (Osaka 2003).
6. Working as a member of the global network “Microbiological Resource Centers”(MIRCENs) operated by UNEP, UNESCO and ICRO; and as an original member of the“Regional Network for Microbiology in Southeast Asia” operated under the auspices of
UNESCO through the Fund-In-Trust of the Japanese Government (J-FIT).
7.

Promoting international cooperative researches in biotechnology with the Southeast
Asian countries related to Biotechnology, under the support of Monbu-kagaku-sho
Grant-in-Aids for International Scientific Research. In addition, ICBiotech cooperates indeveloping international organization and conducting academic seminars related tobiotechnology.

8.

Publishing Annual Reports of ICBiotech periodically.

317

Steering Committee
ChairmanProf. Takuya Nihira (Director of ICBiotech)Committee Members
Prof. Kazuhito Fujiyama (ICBiotech)
Prof. Eiichiro Fukusaki (Graduate School of Engineering)
Prof. Tsuyoshi Inoue (Graduate School of Engineering)
Prof. Hajime Watanabe (Graduate School of Engineering)
Prof. Kazumasa Hirata (Graduate School of Pharmaceutical Sciences)Prof. Masahito Taya (Graduate School of Engineering Science)Prof. Toshihiro Horii (Research Institute for Microbial Diseases)
Staff
Director/Professor
Professor
Adjunct Professor
Collaborative Professor

Dr. Takuya Nihira
Dr. Kazuhito Fujiyama
Dr. Michihiko Ike (Department of Environmental Engineering)
Dr. Watanalai Panbangred (Mahidol University, Thailand)
Dr. Ir. Irfan Dwidya Prijambada(Universitas Gadjah Mada, Indonesia)

Dr. Skorn Mongkolsuk (Mahidol University, Thailand)
Visiting Professor
Dr. Toshiomi Yoshida (Osaka University)
Dr. Tatsuji Seki (Osaka University)
Mr. Junji Tayama (Osaka University)
Visiting Associate Professor Dr. Hiroko Kawasaki (NITE)
Dr. Anak Khantachawana (KMUTT, Thailand)
Visiting Research Scholar
Dr. Kosonh Xayphakatsa (BEI, MOST, Laos)
Dr. Hoang Van Vinh (Vietnam National University, Hanoi)
Associate Professor
Dr. Shigeru Kitani
LecturerDr. Ryo Misaki
Assistant Professor
Dr. Hiroshi Kinoshita
Dr. Takao Ohashi
Administrative Official
Ms. Mutsuko Tsuji
Administrative Assistant
Ms. Fumiko Tomomatsu
Ms. Chiyoko Katayama
Technical Assistant
Ms. Hitomi Yumioka
Ms. Shizuka Kawakami

318

I. COOPERATIVE RESEARCH STATION (CRS) IN SOUTHEAST ASIA
The ICBiotech, Osaka University launched out the Cooperative Research Station (CRS) in
Southeast Asia at Chalermprakiat Building, Faculty of Science, Mahidol University in 2002
through the generous support by Mahidol University. The CRS’s space and equipments are
made available for Southeast Asian and Japanese researchers to undertake cooperative onsite
researches on the development of the abundant natural biological and genetic resources andtheir sustainable utilization in Southeast Asian countries through JSPS core university
program (ended in 2005), JST Special Coordination Funds for Promoting Science andTechnology (2006-2009) and JSPS Asian CORE Program (2009-2014). The CRS alsofunctioned as a lecturing and research station of the UNESCO International Post-graduate
Inter-University program, which Osaka University operated in coordination with Thai
universities. The CRS is considering support to the alumni of Osaka University and provisionof university information for recruitment of students for study in Osaka University.
Mahidol University (MU) and Osaka University (OU) together established the Mahidol
University-Osaka University Collaborative Research Center (MU-OU:CRC) for Bioscienceand Biotechnology at Faculty of Science, Mahidol University in 2002, to strengthen the
research cooperation in these fields which are amongst the most active fields of study andresearch in both universities.
Currently, MU-OU:CRC has coordinated a research projects under the jointly support ofNational Research Council of Thailand (NRCT), National Center for Genetic Engineering andBiotechnology (BIOTEC) and The Japan Society for the Promotion of Science (JSPS).
Researchers from Mahidol University, Chulalongkorn University, Kasetsart University, King
Mongkut's University of Technology Thonburi and BIOTEC participate in this project.
CRS is conducting researches on
1. Strain improvement of Bacillus thuringiensis.
2. Screening of actinomycetes for bioactive compound.
3. The soil metagenome for screening of potential polysaccharide-degrading enzymes.
4. Cell culture system for screening of bioactive compounds from Streptomycetes andenvironmental fungi.
5. Quorum sensing interference: Novel biocontrol strategies for pathogenic bacteria.

II. UNESCO COURSE”UNESCO International Post-Graduate University Course in Microbiology” (1973-2003)
plus the “UNESCO Postgraduate Inter-University Course in Biotechnology” (2004-2007) was
one of the two long-term training courses held in Japan launched in response to therecommendations concluded at a UNESCO General Conference Meeting. After the operationof 33 batches for developing human resources in Asia, total 459 young researchers from allover Asian countries are now playing active and leading roles in current biotechnology
research and education in the region to contribute to the development of their own country,
and consequently to Asian countries, and the establishment of the closer and stronger networkin the region.

319

UNESCO Biotechnology School in Asia (JFIT Project)
The “UNESCO Biotechnology School in Asia” was launched in October 2012. This
program is an activity of the “UNESCO Extra-budgetary Program for Human Resources andResearch Network in Sciences and Technology” supported by the Japanese Fund-In-Trust(JFIT). The course is a program of the scientific training for young Asian universitygraduates in the field of Biotechnology and related areas resulting in achieving master degree,
and the participants are expected to make significant contributions to an educational orscientific institution in the coming years in their home countries. It aims also to strengthen the
network activities among the biotechnology scientists in the region.
Opening Ceremony of the UNESCO Biotechnology School in Asia
“Cohort 2014”
July 28, 2015 at Icho Kaikan, Osaka University
Opening
Welcome address
Address
Address
Address

Prof. Takuya NihiraProf. Toshio Hirano
Dr. Shahbaz Khan
Mr. Dusit Manapan
Prof. Iwan Dwiprahsto

Director, ICBiotech
President, Osaka University
Director, UNESCO Office in Jakarta
Consul General, Royal Thai Consulate-General, Osaka
Vice Rector of Academic Affairs, Universitas GadjahMada, Indonesia

Address

Mr. Wisnu Edi Pratignyo Consul General, Consulate-General, the Republic ofIndonesia in Osaka

Address

Ms. Maria Teresa L. Taguiang

Consul General, Consulate General, theRepublic of the Philippines Osaka-Kobe

Address
Closing
Photo session
Welcome Lunch2014 cohort participants 3 min x 11 participantsProf. Kazuhito Fujiyama ICBiotech[3rd: 2014 cohort students] (Aug 2014 – July 2016)
Nationality

Name

Affiliation

Allocated University

Laos

Sinpakone Singhalath

National U of Laos

ITB, Indonesia

Laos

Niphaphone Boum Venevongsot

National U of Laos

UGM, Indonesia

VietnamNguyen Ngoc Thi Minh

U of Science, HCM

KMUTT, Thailand

Cambodia

Prum Chann Ratha

Royal U of Phnom Penh

KMUTT, Thailand

Cambodia

Sreylen MeasRoyal U of Phnom Penh

KMUTT, Thailand

Laos

Oulay Phoupasong

National U of Laos

KMUTT, Thailand

Mongolia

Dorjkhand.Gunchinbat

Mongolian U of Sci. and Technol.

Mahidol U, Thailand

Laos

Sysouvanh Boubpha

National U of Laos

Chulalongkorn U, Thailand

Thailand

Poungsombat Phornphatcharanan

KMUTT

Nihira Lab, Osaka U

Indonesia

Adinda Darwati Kadar

ITB

Fukusaki Lab, Osaka U

Philippines

Kris Gerard R. Alvarez, R. M.

UPLBNagai Lab, Osaka U

320

Schedule
July 24
July 25
July 27
July 28
July 29

July 30
July 31
August 3
August 4
August 5
August 6

Orientation, Final presentation of 2013 participants
Beer factory (Asahi Beer)
Presentation of Biotechnology Global Human Resource Development Program
Lab Experiment
Opening ceremony, Lab tour / Research Center for Ultra-High Voltage Electron
Microscopy
Lecture (Dr. Kanematsu, Dr. Fujiyama), Workshop on Collaboration between
Osaka U and Japanese companies for global human resource development in thefield of biotechnology
Factory visit (Shimadzu Co.), Culture activity –Kyoto (Shimadzu)
Lecture (Dr. Kawasaki), Seminar (Shimadzu Co.)
Lecture (Dr. Nirianne, Dr. Koga), Cultural activity, Lab experiment
Lecture (Dr. Sastia, Dr. Walter, Dr. Harada), Presentation (2014 Participants)
Team tasks (preparation), Lecture (Dr. Ike), Team tasks (presentation)
Closing ceremony
Leaving Osaka, Japan

III. WORKSHOPThe 3rd Workshop on Collaboration
between Osaka University and Japanese companies

for global human resource development in the field of biotechnology

July 29, 2015

4th floor, International Center for Biotechnology, Osaka University
Sponsorship; International Center for Biotechnology, Osaka University
Co-sponsored; International Research Initiative for Frontier of Industrial Biotechnology, Osaka University

Schedule(13:00-19:00)

13:00-Introduction
14:00- Keynote lecture “Working in a mid-size Japanese Company”
Mazumder Tapan Kumar (YAEGAKI Bio-industry, Inc.)
14:30- Introduction of companies: 1. House Foods Group
2. Ezaki Glico
15:10- Break
15:30- Presentation
1. Ms. Li Menglu (D2, China)
2. Ms. Nursyafiqah Mohamad Ishak (D1, Malaysia)
3. Ms. Ivy Grace Umadhay Pait (D3, Philippines)
4. Ms. Jinjing Li (M2, China)
5. Mr. Tsai Yung Yu (M2, Taiwan)
16:00- Brief introduction of participating companies:
Ajinomoto General Foods, Inc., B.E.Marubishi Co., Ltd., Ezaki Glico Co., Ltd., Hakutsuru

321Sake Brewing Co., Ltd., House Foods Group Inc., Oriental Yeast Co., Ltd., Shionogi & Co.,
Ltd., Sunstar Inc., Suntory Spirits Limited., Takara Bio Inc., Tamanoi Vinegar Co., Ltd., Ueno
Fine Chemicals Industry.
17:20- Mixer (1st floor of ICBiotech)

The 4th Workshop on Collaboration
between Osaka University and Japanese companies

for global human resource development in the field of biotechnology

January 18, 2016
4 and 5 floor, International Center for Biotechnology, Osaka Universitythth

Sponsorship; International Center for Biotechnology, Osaka University
Co-sponsored; International Research Initiative for Frontier of Industrial Biotechnology, Osaka University

Schedule (15:00- 19:30)
15:00- Introduction of companies
1) Ajinomoto General Foods, Inc.
2) Ezaki Glico Co., Ltd.
3) House Foods Group Inc.
4) Fuji Oil Co., Ltd.
15:45- Presentation by international students
1) Frontier Biotechnology (D2, Vietnam)
2) Frontier Biotechnology (D2, Vietnam)
3) Frontier Biotechnology (D2, Bangladesh)
4) Frontier Biotechnology (D2, Indonesia)5) Frontier Biotechnology (M2, Vietnam)
16:25- Introduction of companies
1) Ueno Fine Chemicals Industry, Ltd.
2) Shionogi & Co., Ltd.
3) Toray Industries, Inc.
4) Rohto Pharmaceutical Co., Ltd.
5) Kaneka Corporation
6) Kao Corporation
17:20- Guidance
18:00- Mixer (1st floor of ICBiotech)322

IV. JASSO STUDENT EXCHANGE SUPPORT PROGRAM (SSSV)(Scholarship for Short-Term Visit/ Short-Term Stay Program)This is a field study program jointly operated with GLOCOL (Global Collaboration
Center), Osaka University and several universities in Thailand. In 2015, 20 first year master's
degree students of Osaka University visited 4 universities and 1 institute in Thailand between
August 3 and September 7, and 21 postgraduate students from Thailand visited Osaka
University between September and December, which enhanced mutual interactions.
Students from Osaka U
Name
Kohei Adachi
Asae Takeo
Haruka Yamanoi
Toshiya Tomioka
Chihiro Futoi
Mizuki Yamakuri
Taishi Nakai
Yutaka Minote
Takuto Arao
Jun Miura
Riku Yamamoto
Nobuhiko Kondo
Yurika Mabira
Akiko Maeda
Yuki Obata
Kenji Matsumoto
Kohei Matsunaga
Kohta Nakatani
Daichi Hirata
Yukina Yoshioka

Lab
KanayaNihiraNihiraNihiraNihiraOhtakeHarashima
Fukui
Watanabe
Watanabe
Kino-oka
Kino-oka
Fujiyuama
Fujiyama
Fujiyama
Fujiyama
Fukusaki
Fukusaki
Muranaka
Fujiyama

Students from Thai Universities to Osaka U
Name
Pritsana Sawutdeechaikul
Racha Tabtim
Rapeewan Sowanpreecha
Yutthasin Nopparatpaisit
Marnutsada Tenghoung
Supattra Thammasom
Jeerapan Boonyarit
Monthira Chan-In
Pattaraporn Chantathaweewat
Supaporn Mondech
Pitchapa Rattanaburi
Junya Leiim
Napathip Lertpreedakorn
Tatpong Boontawon

Thai U
CU
CU
CU
CU
Kasetsart U
Kasetsart U
Kasetsart U
Kasetsart U
Kasetsart U
Kasetsart U
Mahidol U
Mahidol U
Mahidol U
Mahidol U

Allocated U
KMUTT
BIOTEC
MU
KMUTT
KU
CU
MU
KU
KMUTT
MU
KMUTT
KUMTT
KU
KU
MU
CU
BIOTECKMUTT
KU
CU

Assigned Supervisor
Dr. Chakrit Tachaapaikoon
Dr. Kobkul Laoteng
Dr. Watanalai Panbangred
Dr. Songsak Wattanachaisaereekul
Dr. Arinthip Tamchaipenet
Dr. Alisa Vangnai
Dr. Chuenchit Boonchird
Dr. Piyada Juntawong
Dr. Werasak Surareungchai
Dr. Puey Ounjai
Dr. Anak Khantachawana
Dr. Kwanchanok Viravaidya
Dr. Savitree Limtong
Dr. Ingorn Kimkong
Dr. Punchapat Sojikul
Dr. Tanapat Palaga
Dr. Sutipa Tanapongpipat
Dr. Nitnipa Soontornkul
Dr. Chatchawan Chantrasuriyarat
Dr. Rungaroon Waditee

Thai Supervisor
Tanapat Palaga
Alisa Vangnai
Panan Rerngsamran
Suchada Chanprateen Napathorn
Arinthip Thamchaipinet
Arinthip Thamchaipinet
Savitree Limtong
Savitree Limtong
Savitree Limtong
Savitree Limtong
Jirarut Wongkongkatep
Thunyarat Pongtharangkul
Chuenchit Boonchird
Chuenchit Boonchird

323

Assigned Supervisor
Dr. Nagai
Dr. Honda
Dr. Nihira
Dr. Muranaka
Dr. Muranaka
Dr. Nihira
Dr. Fujiyama
Dr. Watanabe
Dr. Fujiyama
Dr. Fujiyama
Dr. Fukusaki
Dr. Honda
Dr. Sugiyama
Dr. Fujiyama

Suphanun Phuphanitcharoenkun

Adtaporn Poonsawas
Phakhinee Thianheng
Kulnipa Tanarungrangsee
Pimploy Ratanaamnuaychai
Sira Jampa-ngern
Chuaykhongthong Thitapha

Mahidol U
KMUTT
KMUTT
KMUTT
KMUTT
KMUTT
CU

Suparerk Borwornpinyo
Nitnipa Soontorngun
Khanok Ratanakhanokchai
Natta Laohakunjit
Yaowaluck Maprang Roshorm
Anak Khantachawana
Panan RerngsamranDr. Kino-oka
Dr. Sugiyama
Dr. Fujiyama
Dr. Nihira
Dr. Fukui
Dr. Kino-oka
Dr. Fujiyama

V. JST Japan-Asia Youth Exchange Program in Science(SAKURA Exchange Program in Science)
Purpose of the Program: Promoting science and technology is a key engine to materializea bright future of Asia and it is vitally important to enhance the exchange of youths in Asian
countries and Japan who will play a crucial role in the field of science and technology. Basedon this concept, “Japan-Asia Youth Exchange Program in Science” (SAKURA Exchange
Program in Science) is the program for enhancing exchanges between Asia and Japan of the
youths who will play a crucial role in the future field of science and technology through theclose collaboration of industry-academia-government by facilitating short-term visits ofcompetent Asian youths to Japan. This program aims at raising the interest of Asian youths
toward the leading Japanese science and technologies at Japanese universities, research
institutions and private companies.
List of students (September 6-15, 2015)
Country

NameCountryName

Lao PDR

Thanh Phommasack

Mongolia

Ariya Banzragchgarav

Lao PDR

Thiphachanh Suvanasan

Mongolia

Bataa Dolgormaa

Cambodia

Sok Bophal

TaiwanTzu-Hsien Yeh

Cambodia

Mom Panha

TaiwanKuan-Heng Tu

Brunei

Siti Hakimah Binti S. M. Musa

TaiwanWei LuSchedule
September 7
September 8
September 9
September 10
September 11
September 14

Orientation
Lecture: Industrial application of enzymes (Dr. Kenji Okano)Lab tour at Nihira Lab, ICBiotech.
Lab tour: Riken Spring 8, Super Computer “Kei”
Visiting company: Shimadzu
Presentation of task, certificate award324

VI. SCIENTIST EXCHANGES
Record of Scientist Exchange (FY2015)
From Japan to counterpart countries
Name

Takuya Nihira

Kaazuhito Fujiyama

Shigeru Kitani

Affiliation

Visiting Period
Apr. 4 – 13, 2015

ThailandMay 31 – June 10, 2015

ThailandJuly 10 - 12, 2015

Thailand

Aug. 3 - 10, 2015

Thailand

Aug. 31 – Sept. 5, 2015

Thailand

Director, Prof., ICBiotech,Oct. 6 – 13, 2015
Osaka U

Prof., ICBiotech,
Osaka U

Assoc. Prof., ICBiotech

Host Organization

ThailandNov. 8 – 14, 2015

Philippines

Nov. 16 – 17, 2015

ThailandJan. 27 – Feb. 3, 2016

Vietnam, Thailand

Mar. 9 – 16, 2016

Thailand

Mar. 21 – 24, 2016

ThailandJune 25-28, 2015

VietnamAug. 31 – Sept. 5, 2015

Aachen TH, Germany

Oct. 8 – 11, 2015

ThailandNov. 15 – 19, 2015

Indonesia, Thailand

Feb. 28 – Mar. 4, 2016

Thailand

Oct. 29 – Nov. 2, 2015

VietnamDec. 13 – 19, 2015

ThailandJune 25-28, 2015

VietnamRyo Misaki

Lecturer, ICBiotech

Asae Takeo

Nihira Lab

BIOTEC, Thailand

Kohei Matsunaga

Fukusaki Lab

BIOTEC, Thailand

Mizuki Yamakuri

Ohtake Lab

CU, Thailand

Kenji Matsumoto

Fujiyama Lab

CU, Thailand

Yukina Yoshioka

Fujiyama Lab

Kohei Adachi

Kanaya Lab

Toshiya Tomioka

Nihira Lab

Takuto Arao

Watanabe Lab

Riku Yamamoto

Kino-oka Lab(JASSO)
SSSV
Aug. 3- Sept. 7, 2015

CU, Thailand
KMUTT, Thailand
KMUTT, Thailand
KMUTT, Thailand
KMUTT, Thailand

325

Nobuhiko Kondo

Kino-oka Lab

KUMTT, Thailand

Kohta Nakatani

Fukusaki Lab

KMUTT, Thailand

Chihiro Futoi

Nihira Lab

KU, Thailand

Yutaka Minote

Fukui LabKU, Thailand

Yurika Mabira

Fujiyuama Lab(JASSO)KU, Thailand

Akiko Maeda

Fujiyama Lab

SSSV

KU, Thailand

Daichi Hirata

Muranaka Lab

Aug. 3- Sept. 7, 2015

KU, Thailand

Haruka Yamanoi

Nihira Lab

MU, Thailand

Taishi Nakai

Harashima Lab

MU, Thailand

Jun Miura

Watanabe Lab

MU, Thailand

Yuki Obata

Fujiyama Lab

MU, Thailand

Between counterpart countries
Name

Affiliation

Visiting Period

Host Organization

High School Students

India, Thailand, Philippines

May 11, 2015

JST Sakura Sci. Program

Haseena Khan

U of Dhaka, Bangladesh

May 12-16, 2015

Nihira Lab., ICBiotech

Mohammad Riazul Islam

Inst. for Med. Microbiol., Immunol.
& Parasitol., Bangladesh

May 12-16, 2015

Nihira Lab., ICBiotech

Pirapan Polburee

Kasetsart U, Thailand

June 15, July 16, 2015

Internship Program

Jasmine Corbin

UC, Davis, USA

June 16, 2015

Fujiyama Lab. (JSPS)

Vo Thi Hong Phuong

VietnamJuly 7, Aug. 6, 2015

Internship Program

Thanh Phommasack

National U of Laos

Thiphachanh Suvanasan

National U of Laos

Sok Bophal

Royal U of Phnom Penh

Mom Panha

Royal U of Phnom Penh

Siti Hakimah Binti S. M.
Musa

U Brunei Darussalam(JST)
SAKURA
Science Program

Ariya Banzragchgarav

National U of Mongolia

Sept. 6-15, 2015

Bataa Dolgormaa

National U of Mongolia

Tzu-Hsien Yeh

Natl Cheng Kung U, Taiwan

Kuan-Heng Tu

Natl Tsing Hua U, Taiwan

Wei LuNatl Tsing Hua U, Taiwan

326

ICBiotechPritsana Sawutdeechaikul CU, Thailand

Nagai Lab., Osaka U

Racha Tabtim

CU, Thailand

Honda Lab., Osaka U

Rapeewan Sowanpreecha

CU, Thailand

Nihira Lab., ICBiotech

Yutthasin Nopparatpaisit

CU, Thailand

Muranaka Lab., Osaka U

Marnutsada Tenghoung

Kasetsart U, Thailand

Muranaka Lab., Osaka U

Supattra Thammasom

Kasetsart U, Thailand

Nihira Lab., ICBiotech

Jeerapan Boonyarit

Kasetsart U, Thailand

Fujiyama Lab., ICBiotech

Monthira Chan-In

Kasetsart U, Thailand

Watanabe Lab., Osaka U

Pattaraporn
Chantathaweewat

Kasetsart U, Thailand(JASSO)

Fujiyama Lab., ICBiotech

Supaporn Mondech

Kasetsart U, Thailand

SSSV

Fujiyama Lab., ICBiotech

Pitchapa Rattanaburi

Mahidol U, Thailand

Junya Leiim

Mahidol U, Thailand

Napathip Lertpreedakorn

Mahidol U, Thailand

Tatpong Boontawon

Mahidol U, Thailand

Fujiyama Lab., ICBiotech

Suphanun
Phuphanitcharoenkun

Mahidol U, Thailand

Kino-oka Lab., Osaka U

Adtaporn Poonsawas

KMUTT, Thailand

Sugiyama Lab., Osaka U

Phakhinee Thianheng

KMUTT, Thailand

Fujiyama Lab., ICBiotech

Sept. – Dec., 2015
(Periods are different
in each student)

Fukusaki Lab., Osaka U
Honda Lab., Osaka U
Sugiyama Lab., Osaka U

Kulnipa Tanarungrangsee KMUTT, Thailand

Nihira Lab., ICBiotech

Pimploy
Ratanaamnuaychai

KMUTT, Thailand

Fukui Lab., Osaka U

Sira Jampa-ngern

KMUTT, Thailand. Kino-oka Lab., Osaka U

ChuaykhongthongThitapha

CU, Thailand

Fujiyama Lab., ICBiotech

Irma Isnafia Arief

Bogor Agric. U. Indonesia

Oct. 26 – Nov 20, 2015

Fujiyama Lab., ICBiotech

Raymond Rodriguez

UC, Davis

Dec. 8-11, 2015

Seminar at ICBiotech

Ninh Thi Ngoc

VAST, Vietnam

Jan. 7- Feb 4, 2016

Fujiyama Lab., ICBiotech

Kosonh Xayphakatsa

BEI, LaosJan. 7, 2016

Nihira Lab., ICBiotech

Ir. Liliek Sulistyowati

Brawijaya U, Indonesia

Feb. 4, 2016

Nihira Lab., ICBiotech

Hagus Tarno

Brawijaya U, Indonesia

Feb. 4, 2016

Nihira Lab., ICBIotech

Hoang Van Vinh

Vietnam National U

Feb. 17, 2016

Nihira Lab., ICBiotech

Pannamthip Pitaksajjakul

Mahidol U, Thailand

March 1, 2016

Fujiyama Lab., ICBiotech

Wilarat Puangmanee

Mahidol U, Thailand

March 1, 2016

Fujiyama Lab., ICBiotech

327

VII. GUESTS/VISITORS
Name

Affiliation

Staying Period

Skorn Mongkolsulk

Mahidol U, Thailand

April 21, 2015

Patcharee Lertrit

Mahidol U, Thailand

April 21, 2015

Watanalai Panbangred

Mahidol U, Thailand

April 21, 2015

Boonchaoen Sirinaovakul

KMUTT, Thailand

April 21, 2015

Boosaya Bunnag

KMUTT, Thailand

April 21, 2015

Anak Khantachawana

KMUTT, Thailand

April 21, 2015

85 High School students

Taipei Municipal Jianguo Senior High School,
TaiwanApril 24, 2015

Sung Been Moon

Yonsei U, Korea

May 11, 2015

Yun Mook Lim

Yonsei U, Korea

May 11, 2015

Jung Sook Lee

Yonsei U, Korea

May 11, 2015

Haesook Youm

Yonsei U, Korea

May 11, 2015

Wang Sub Lee

Yonsei U, Korea

May 11, 2015

Soojin Kim

Yonsei U, Korea

May 11, 2015

Somchai Chauvatcharin

Mahidol U, Thailand

June 24, 2015

Opor Sonpung

KMUTT, Thailand

July 7, 2015

Anak Khantachawana

KMUTT, Thailand

July 16, 2015

Shabaz Kahn

UNESCO Jakarta Office

July 28, 2015

Iwan Dwiprahsto

U of Gadja Mada, Indonesia

July 28, 2015

Maria Teresa L. Taguiang

Philippine Consulate General

July 28, 2015

Wan Wardatul Amani Binti Wan
Salim

International Islamic U Malaysia

Aug. 6, 2015

Noor Illi Mohamad Puad

International Islamic U Malaysia

Aug. 6, 2015

Hadi Purwanto

International Islamic U Malaysia

Aug. 6, 2015

B.A.Chopade

Dr.Babasaheb Ambedkar Marathwada U

Sept. 9, 2015

Jian-Jiang Zhong

Shanghai Jiao Tong U, China

Oct. 30, 2015

Ir. Syamsidah Rahmawati

Lembaga Ilmu Pengetahuan Indonesia

Dec. 11, 2015

Yopi

Lembaga Ilmu Pengetahuan Indonesia

Dec. 11, 2015

Nguyen Van Be

Can Tho U, Vietnam

March 3, 2016

Nguyen Vo Chau Ngan

Can Tho U, Vietnam

March 3, 2016

Van Pham Dan Thuy

Can Tho U, Vietnam

March 3, 2016328

Nguyen Van Cong

Can Tho U, Vietnam

March 3, 2016

Nguyen Cong Ha

Can Tho U, Vietnam

March 3, 2016

Duong Minh Vien

Can Tho U, Vietnam

March 3, 2016

Nelson B. Arboleda Jr.

De La Salle U, Philippines

March 25, 2016

Melanie Y. DavidDe La Salle U, Philippines

March 25, 2016

Emelina H. Mandia

De La Salle U, Philippines

March 25, 2016

Bambang Wibawarata

U of Indonesia

March 31, 2016

Rosari Saleh

U of Indonesia

March 31, 2016

Sri Mardiyati

U of Indonesia

March 31, 2016

Heri Hermansyah

U of Indonesia

March 31, 2016

VIII. COMMITTEE MEETING
Steering Committee Meetings of ICBiotech were convened as follows:■July 17, 2015: Discussion on inviting a Visiting Research Scholar and assigning title of professor:
Mr. Junji Tayama, Japan (August 1, 2015 – March 31, 2016).
■September 9, 2015: Discussion on inviting a Visiting Research Scholar:
Ms. Dian Anggraini Suroto, Indonesia (October 1, 2015 – September 30, 2016).
■October 27, 2015: Discussion on inviting a Visiting Research Scholar:
Dr. Kosonh Xayphakatsa, Laos (January 8, 2016 – February 9, 2016),
Dr. Hoang Van Vinh, Vietnam (February 17, 2016 – March 18, 2016).
■December 3, 2015: Discussion on inviting a Part-time Lecturer:
Dr. Shigenori Kanaya, Japan (April 1, 2016 – March 31, 2017).
■March 7, 2016:
Report on:
・UNESCO Biotechnology School in Asia and its opening ceremony
・JST Sakura Exchange Program in Science・3rd and 4th Workshops (29th July 2015, and 18th January 2016) on Collaboration
between Osaka University and Japanese Companies
・JASSO Short-stay and Short-visit program 2015 (3rd August – 7th September, 2015)
・Two Visiting Research Scholars (Dr. Kosonh Xayphakatsa from Laos andDr. Hoang Van Vinh from Vietnam) staying at ICBiotech during the FY2015
・Adjunct Professor for the FY2016
・Evaluation committee meeting (7th March 2016)
Discussion on:
・Selection of members of the evaluation committee
・Conferring the title on visiting professors from abroad for FY2016
・Conferring the title on visiting professors assigned from Japanese universities for FY2016
・Inviting a visiting research scholar329