LAM10/16/25 Locomotor Activity Monitor
The LAM10/16/25 Locomotor Activity
Monitor uses infrared beams tocharacterize the pattern of movement ineach of 32 individual tubes or vials.
A planar array of 3 or 9 radial beams
crosses each tube at its midpoint, andwhen animals in the tube move through thearray, the beams are interrupted and thenregistered as counts. Over the course of anexperiment, which may last for days orweeks, the counting circuits continuously
monitor each tube for activity, and at
periodic intervals upload their count totals
to the host computer for storage andanalysis. This daily record provides a goodmeasure of both the intensity of locomotor
activity and the relative periods of rest ineach tube.

LAM25HFeatures• 32 tubes of 10, 16, or 25 mm diameter• 3 or 9 consolidated IR beams per tube
• Horizontal or vertical tube orientation• Consistent operation in bright room light

The unit is available in 3 tube sizes: 10, 16,
and 25mm diameter, and either 3 or 9beams. The 3-beam unit is appropriate foranimal species with a standing height of atleast 1/2 of the tube diameter. Otherwise the9-beam high-resolution unit should be used
to insure reliable detection.

or darkness• Ambient light sensor provides on/oﬀ

record of entrainment cycles, stimulation
pulses, and inadvertent light exposure.

Populations of drosophila melanogaster up
to about 20 individuals may be accurately
characterized by the LAM25H, which uses
25mm vials and 9 beams.

Specifications• Tube diameter: 10, 16, or 25mm
• Unit Dimensions: 13.0 x 4.7 x 7.8” (33 x
12 x 20 cm) LWH, without tubes

These units may be operated withhorizontal tubes as shown, or rotated back90 degrees to orient the vials vertically. Anoptional tube support plate mounts on therear of the unit to register and support thetubes when vertical.

• Mass: 0.8 kg without tubes• Ambient Light Sensor threshold: 10 lux
nominal, photopic response curve

A 3-board version of each unit is available
for additional measurement fidelity. These 3board stacks, the LAM10H-3. LAM16H-3,and LAM25H-3, measure the activity in eachtube with 3 independent beam arrays
spaced at 10mm along the tube axis.

• Interconnect: 4 wire, 6 position, RJ-11
modular telephone line jack to PSIU9DAMSystem network for 9V DC power
input and data transmission• Data collection software: DAMSystem3• Operating environment: normallaboratory, non-condensing

The unit is compatible with the PSIU9
hardware network and the DAMSystem3
data collection software application. Up to120 monitors may be connected to a single
system of this type.

TRIKINETICS
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Available Models
3-beam: LAM10, LAM16, LAM25
9-beam: LAM10H, LAM16H, LAM25H
3-board: LAM10H-3, LAM16H-3, LAM25H-3
Vertical Tube Support plate: LAMVTS

Tubes and Caps
We can supply tubes in polished Corning glass of
10, 16, or 25mm diameter which are open on bothends. Standard scientific vials in 10, 16, or 25mm
size may also be used, in either a plastic or glassmaterial.

LAM10H-3 in vertical orientation withvertical tube support

We can also supply vinyl tube caps in the 10, 16, and 25mm diameters.

Monitor Setup
Insert the tubes through the 32 holes in the monitor, and leave them centered so that thedetection beam array will bisect the tube.
If captivation of the tubes is necessary to prevent sliding, use rubber bands, stretched firstover the 4 corner tubes of 2 adjacent rows, and pressed up against the monitor surface. Thenremove the tubes from columns 3 and 6, and reinsert them from above and below the bands asshown to place them into frictional contact.

Tube restraint

With the tubes installed, the monitor may be plugged into the DAMSystem network.
Units with the vial support plate may be operated in either a vertical or horizontal orientation.
The plate’s location or height may be adjusted by moving the nut pairs in parallel on each of
the 4 screws. Tighten to snug by hand only.

Ambient Light Sensor
The ambient light sensor is set to discriminate light from dark at a nominal threshold of 10 lux,and will report its output with the count data at each collection bin. With Display Select set toCurrent Data (or Live) and #nnn, the sensor output will be displayed in real time, and may be
used for diagnostic purposes. When File data is retrieved from the monitor at the end of eachbin, the light sensor will report dark(0) only if dark for the entire preceding bin. If the sensor
measures light above its threshold at any point during the bin, it will report light(1) for the bin,serving to detect door openings or other lights-on transients.
The sensor output (1 or 0) is reported in column 10 of the MonitorNNN file.

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Data Collection
To verify that the monitor is operating properly, select Current Data (or Live) for the single
monitor (#nnn) in question in the DAMSystem3 host application. The status box should begreen (status 1), and the 32 channels of real-time count activity should be displayed. If a thinobject such as a pencil point is moved through one of the tube cavities, the count total for thatchannel should increment.
The monitor will accumulate activity counts for as long as it has operating power, and will
uplink its accumulated counts (and then reset to 0) whenever commanded to do so by the hostcomputer. Counts will be accumulated in both total darkness and bright ambient light.
3-board units report data as 3 independent monitors, numbered consecutively from thebottom or rear board to the top or front board. The label shows the number for the middleboard.

Precautions
Beware of operation in proximity to incandescent light, where stray infrared illumination caneasily interfere with the exposed LAM detectors. Perform an empty-tube run to verify zero
counts prior to operation with live specimens, and switch to LED lights if necessary.
Beware of liquid spills onto the green circuit board. Unplug the unit immediately if water or
any liquid drips inside, and dry thoroughly in a warm (40C) oven prior to reuse.
Additional information is available in the Help section of the DAMSystem3 data collection
program.

LAM50 Locomotor Activity Monitor
The LAM50 Locomotor Activity
Monitor is a scaled-up version of theLAM25, providing monitoring
capability for larger animals which
require 50mm diameter tubes.
It is available with 9-beams pertube, contains the ambient lightsensor, and connects to the PSIU9/DAMSystem3 data collection
system.
Plastic tubes and caps areavailable.

LAM50HSpecifications• 32 tubes, 50mm diameter• 9 consolidated infrared beams per tube
• Dimensions: 23.0 x 5.0 x 13.0” (58 x 13 x 33 cm) LWH, without tubes• Mass: 3.6 kg without tubes• Ambient Light Sensor threshold: 10 lux nominal, photopic response curve• Interconnect: 4 wire, 6 position, RJ-11 modular telephone line jack to PSIU9 DAMSystem3
network for 9V DC power input and data transmission

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