Anti-HIV-1 Protease antibody, rabbit serum
Product code65-018

Size

100 µlStorage

Store at 4℃ for short term. For long term storage store at -20℃.
Aliquot to avoid repeated freezing and thawing.

Concentration

N/A

BufferPreservative: 0.09% sodium azide

PurityRabbit antiserum

Immunogen

Full-size functional recombinant HIV-1 protease expressed and purified from E.

coliIsotype

Rabbit IgG

Reactivity

HIV-1 protease of all substrains

Special notes

N/A

Application

1.

Western blotting (1/2,000)

2.

Dot blotting (1/2,000)

3.

Immunoprecipitation (1/200)

4.

Inhibition of HIV-1 Protease activity (assay dependent)

5.

ELISA. (1/10,000)
Other applications have not been tested.

BackgroundHIV-1 protease is the aspartyl protease that mediates proteolytic cleavages ofGag and Gag-Pol polyproteins during or shortly after the release of the virion
from the plasma membrane. Cleavages take place as an ordered, step-wisecascade to yield mature proteins. This process is called maturation. Displays
maximal activity during the budding process just prior to particle release fromthe cell. Also cleaves Nef and Vif, probably concomitantly with viral structuralproteins on maturation of virus particles. Hydrolyzes host EIF4GI and PABP1
in order to shut off the capped cellular mRNA translation. The resulting
inhibition of cellular protein synthesis serves to ensure maximal viral gene
expression and to evade host immune response.

Data Link

UniProtKB P03367 (gag-pol), Q9YQ30 (HIV-1 Protease)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 65-018 Anti-HIV-1 Protease antibody, rabbit serum

Fig.1 Detection of HIV-1 proteinase in the extract of HIV-1 infected cells by Western blotting using
anti-HIV-1 protease antibody.
1: Purified HIV-1 protease (1 ng, BioAcademia 05-013)
2: Extract of MT4 cells
3.: Extract of MT4 cells infected with HIV-1 (LAI strain)
The antiserum was used at 1/2,000 dilution.

Fig.2. Dot blotting of HIV-1 protease by using anti-HIV-1 protease antibody.
Anti-HIV-1 protease antibody was used at 1/2,000 dilution. As second antibody, goat anti-rabbit IgG
antibody conjugated with HRP was used at 1/5,000 dilution. ECL system was used.

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Fig.3 ELISA of HIV-1 protease with anti-HIV-1 protease antibody.
The antibody was used at dilutions indicated above. Purified Protease was spotted on wells.
References： The HIV-1 strain and immunogen has been described in the following references.
1. Adachi A et al “Production of acquired immunodeficiency syndrome-associated retrovirus inhuman and nonhuman cells transfected with an infectious molecular clone” J Virol 59: 284 291(1986) PMID: 3016298
2. Saitoh A et al “Overproduction of human immunodeficiency virus type I reverse transcriptase in
Escherichia coli and purification of the enzyme” Microbiol Immunol 34:509-521 (1990) PMID:
1699113

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Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/