Anti-FcεR1α (human IgE receptor) antibody, mouse monoclonal (CRA1) (biotin)
Product code72-003

Size

50 µg

Storage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

ImmunogenPurified recombinant extracellular portion of human FcεR1α (corresponding toamino acids Met-26-197, where signal peptide is 1-25)

Isotype

Mouse IgG 2bReactivity

Human, house musk shrew

Special notes

Epitope: 26-110 amino acids
Conjugation: biotin ([biotin]/[IgG] = 8.9; Lot dependent)

Application

1. Western blotting (～1 µg/ml)
2. Flow Cytometry (FC) (1~5 µg/ml)
3. IHC-P, IHC-F (~1 µg/ml)
4. Titration of IgE-bound receptor in combination with CRA2 antibody (Ref.3)
5. ELISA

BackgroundFcεRIα is subunit of the high affinity receptor for IgE to which IgE directly
binds. FcεRIα is a tetrameric complex consisting of one α, one β and two γ
subunits. The latter two are required for signal transduction activity. The
FcεRIα complex plays an important role in triggering allergic responses.
The CRA1 (AER37) monoclonal antibody reacts with the FcεRIα subunit on aregion that does not overlap the region of the IgE binding site, thus it does notcompete with IgE for the receptor binding. Since the CRA2 (AER24) monoclonal
antibody reacts with the IgE binding site on FcεRIα, it competes with IgE forthe receptor binding. Combining the two antibodies, one can quantitatively
measure the amounts of the IgE-bound FcεRIα.

Data Link

UniProtKB/Swiss-Prot P12319 (FCERA_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 72-003 Anti-FcεR1α (human IgE receptor) antibody, mouse monoclonal (CRA1) (biotin)

Fig.1 Flow-cytometry analysis with anti- FcεRIα antibody (CRA1), biotin-conjugated.
U266 cells were incubated with recombinant soluble FcεRIα and further reacted with biotinconjugated anti-FcεRIα antibody (CRA1), followed by PE-conjugated streptavidin. The stained cells
were analyzed by flow cytometry.
References: This antibody has been used in the following publications.
1. Suzuki K et al. The Fc receptor (FcR) γ subunit is essential for IgE-binding activity of cell-surface
expressed chimeric receptor molecules constructed from human high-affinity IgE receptor(FcεRI) αand FcRγ subunits. Mol Immunol. 1998 Apr;35(5):259-70. PMID: 9747886. WB (human)
2. Yanagihara Y. et al. Recombinant soluble form of the human high-affinity immunoglobulin E (IgE)
receptor inhibits IgE production through its specific binding to IgE-bearing B cells. J Clin Invest.
1994 Nov; 94(5): 2162–2165. doi: 10.1172/JCI117574 PMCID: PMC294671. FC (human)
3. Hayashi S et al. Detection of anti-IgE and anti-FcɛRI α chain auto-antibodies in patients withatopic dermatitis. Allergology International Volume 49, Issue 1, 2000, Pages 47-54.

ELISA(human)
4. Yoshimura-Uchiyama C. et al. Comparative effects of basophil-directed growth factors BiochemBiophys Res Commun. 2003 Mar 7;302(2):201-6. PMID:12604332 FC (human)
Related product72-001 Anti-FcεR1α (human IgE receptor) antibody, mouse monoclonal (CRA1)
72-004 Anti-FcεR1α (human IgE receptor) antibody, mouse monoclonal (CRA1) (FITC)
72-005 Anti-FcεR1α (human IgE receptor) antibody, mouse monoclonal (CRA2)
72-007 Anti-FcεR1α (human IgE receptor) antibody, mouse monoclonal (CRA2) (biotin)
72-008 Anti-FcεR1α (human IgE receptor) antibody, mouse monoclonal (CRA2) (FITC)

2 / 2BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/