Anti-RRM2 / RNR-R2 antibody, rabbit polyclonal
Product code70-050Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityAffinity-purified with immunogen.

ImmunogenSynthetic peptide (11 amino acids) in the C-terminal region of human andmouse RRM2, conjugated with KLH. The exact sequence is commerciallysensitive.

IsotypeRabbit IgG

Reactivity

Human, mouse, rat, hamster and xenopus. Not tested in other species.

Special notes

N/AApplication

1. Western blotting ( 1/1,000-1/2,000 dilution)
2. Immunoprecipitation (1/300-1/1,000 dilution)
3. Immunofluorescence staining (1/100-1/1,000 dilution)
4. Immunohistochemistry; paraffin section (1/300 dilution)

Background

Ribonucleoside-diphosphate reductase subunit M2 (RRM2; 389 aa, 45 kDa) also
known as ribonucleotide reductase subunit R2 (RNR-R2), is a rate-limiting
subunit of an enzyme that catalyzes the formation of deoxyribonucleotides fromribonucleotides.

Deoxyribonucleotides in turn are used in the synthesis ofDNA. Furthermore RNR plays a critical role in regulating the total rate of DNA
synthesis so that DNA to cell mass is maintained at a constant ratio during cell
division and DNA repair. It has been shown that MMR2 undergoes
phosphorylation at Ser20 and Thr33.

Data Link

UniProtKB/Swiss-Prot P31350 (RIR2_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
1 / 4

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 70-050 Anti-RRM2 / RNR-R2 antibody, rabbit polyclonal

Fig.1 Western blot analysis of endogenous RRM2 in crude cell extracts
1. HeLa cells (20 µg)
2. MCF7 cells (20 µg)
3. NIH3T3 cells (20 µg)
4. Xenopus eggs at mitotic stage (20 µg)
Multiple bands are due to phosphorylation at Ser20 and/or Thr33 (human sequence). The antibody
was used at 1/1,000 dilution

2 / 4

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Fig.2 Immunoprecipitation of RRM2 from CHO cells.
Lane 1; Crude extract of CHO cells
Lane 2; The immunoprecipitate with the antibody at 1/1,000 dilution.
Lane 3; Supernatant of immuno –precipitation.
The upper band in lane 2 is IgG heavy chain
The antibody was used at 1/500 dilution

Fig.3

Immunofluorescence staining of RRM2 protein in MCF cells with anti-RRM2 antibody.

MCF7 cells were fixed with 4%PFA and permeabilized with 0.25% TritonX 100 and reacted with antiRRM2 antibody at 1/100 dilution. As the second antibody, anti-rabbit IgG antibody conjugated withAlexa Fluor 488 (Abcam) was used at 1/1,000 dilution. DNA was stained with 1.0μg/mL DAPI in TBS.

3 / 4

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Fig.4 Immunohistochemical staining of RRM2 in mouse testis with anti-RRM2 antibody.
Section of formalin-fixed and paraffin embedded mouse testis was reacted with anti-RRM2 antibody
at 1/300 dilution. Nuclear DNA was stained with DAPI (center) and merged image is shown on left.
RRM2 is abundantly expressed in actively proliferating cells

Reference : This product was used in the following publication.
1. Takada S. et al. Identification of ribonucleotide reductase protein R1 as an activator of
microtubule nucleation in Xenopus egg mitotic extracts.

Mol Biol. Cell 11,: 41734187 (2000)

PMID: 11102516

4 / 4

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/