Anti-Tektin-2/Tectin-t antibody, rabbit polyclonal
Product code73-071Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from rabbit antiserum

ImmunogenPurified Tektin-2 with Hit tag expressed in E.coli.

IsotypeRabbit IgG

Reactivity

Human, Mouse

Special notes

N/A

Application

1. Western blotting (1-10µg/ml)
2. Immunofluorescence staining (1-20µg/ml)

Background

Tektins are a class of proteins that form filamentous polymers in the walls ofciliary and flagellar microtubules. Tektin-2 (tektin-t) is a member of the tektinfamily, identified from a mouse haploid germ cell-specific cDNA library. In mice,
tektin-t protein has been localized to the tail of mature sperm. A humanorthologue of mouse haploid germ cell-specific tektin-2 showed 82 and 83%
identity with mouse tektin-2 respectively. Included were a sequence conservedin the tektin B1 family, the TEKTIN2 motif, and the consensus sequence in thetektin protein family composed of nona-peptide. Human tektin-2 protein,having a molecular weight of 54 kDa, was exclusively expressed in the testis,whereas two additional stronger bands of 46 and 56 kDa existed in sperm. The
h-tektin-2 localized specifically to the principal piece of flagella and to the postacrosomal region. The h-tektin-2 gene was assigned to chromosome 1 by aradiation hybrid mapping technique.
Molecular mass: Mouse 50 kDa

Data Link

UniPlotKB Q922G7 (TEKT2_MOUSE)Q9UIF3 (TEKT2_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: Anti-Tektin-2 / Tectin-t antibody, rabbit polyclonal

Fig.1 Western Blot of Tektin-2 protein
Applied sample; 50µg of mouse testis whole lysate.
Primary antibody; 1µg/ml of anti-Tektin-2 (Tektin-t) antibody
Secondary antibody; 1/10,000 dilution of goat anti-rabbit IgG antibody HRP-conjugated, ab97051)
Molecular mass of Tektin-2; 50 kDa

Fig.2 Western blot analysis of h-tektin-t in human organ. Approximately 50 μg of protein sample wasloaded in each lane, separated by 10% SDS-PAGE, transferred onto PVDF membrane. The membrane
was incubated in 5% skim milk for blocking and reacted with anti-Tektin-t antiserum diluted 1,000
fold with TBS. Goat anti-rabbit IgG antibody conjugated with HRP was used as the second antibody.

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Fig.3 Immunofluorescence microscopic images of human sperm. Human sperm were immunostainedwith anti-h-tektin-t rabbit antiserum. As a control, pre-immune rabbit serum was used (control).
Phase contrast microscopic images of the sperm are presented in the lower panel. Original
magnification is ×1000. Scale bar = 50 μm.

Human sperm samples were separated from semen by washing with phosphate-buffered saline and spotted
onto slide glass. The sperm samples were fixed with 100% EtOH for 10 min on ice. After blocking, theslides were incubated with anti-h-tektin-t rabbit antiserum (diluted 1:1000) overnight at 4°C and thentreated with 5% normal donkey serum for 30 min at room temperature. Then the slides were incubatedwith FITC-conjugated anti-rabbit IgG antibody (1:500) for 2 h at room temperature. The samples were
examined under a fluorescence microscope.
Reference: This product has been used in the following publication (Ref.2, 3).
1. Iguchi N, Tanaka H, Nishimune Y, et al. Molecular cloning of haploid germ cell-specific tektin
cDNA and analysis of the protein in mouse testis. FEBS Letters 456(2)315-321(1999) PMID:11870087. WB, IF
2. Tanaka H, Nishimune Y, et al. Mice deficient in the axonemal protein Tektin-t exhibit maleinfertility and immotile-cilium syndrome due to impaired inner arm dynein function. Mol Cell Biol
18 7958-7964 (2004) PMID: 15340058. WB, IF (mouse)
3. Iguchi N, Tanaka H, Nishimune Y, et al. Cloning and characterization of the human tektin-t gene.
Mol Hum Reprod (6)525-530 (2002) PMID: 12029069. WB, IF (human)

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BioAcademia,Inc. Tel. 81-6-6877-2335

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