2019 International Symposium on

Interdisciplinary Pulsed Power
Date ： March 8, 2019 (Fri.) 9：00-12：05
Venue： Kurokami South Campus, Kumamoto University
Kurokami South W3 Bldg. 2F, Lecture Room 203
http://ewww.kumamoto-u.ac.jp/en/about/access/campus/

Scope

In recent years, merits of extremely energetic states or extremely nonequilibrium states produced by pulsed power technology have been
appreciated scientifically, as a result, their applications are expanding invarious directions. This symposium provides an opportunity to share the newtrends in cutting edge pulsed power technologies for biotechnology andbiomedical treatment, from the basics to the applications, which arepresented by four world’s distinguished Professors.

Program
9:00

Opening Address: Prof. Hokamoto (IPPS, Kumamoto Univ.)
Session I

Chair: Prof. Hamid Hosano (IPPS)

9:05

Prof. Marie-Pierre Rols (Univ. of Toulouse, CNRS, France)“Electropermeabilization: Physics for Biology”

9:50

Prof. Gregor Sersa (Inst. of Oncology Ljubljana, Slovenia)“Biomedical Application of Electroporation:
From Preclinical Research to Clinical Practice”

Prof. Rols

- Break -Session II

Chair: Prof. Sunao Katsuki (IPPS)

10:50

Prof. Ken-ichi Yano (IPPS, Kumamoto University)“Activation of Immune Cells
by Nanosecond Pulsed Electric Fields”

11:20

Prof. A.J.M. Pemen(Eindhoven University of Technology, The Netherlands)

Prof. Sersa

Prof. Yano

“Pulsed Power for Bioelectrics
and Environmental Applications”
12:05

Closing

Organized by Institute of Pulsd Power Science (IPPS), Kumamoto University
INQUIRY: katsuki@cs.kumamoto-u.ac.jp

Prof. Pemen

2018 International Symposium on Interdisciplinary Pulsed PowerABSTRACTs“Electropermeabilization: Physics for Biology”
Prof. Marie-Pierre Rols
University of Toulouse, CNRS, France
Marie-Pierre.Rols@ipbs.frCell membranes can be transiently permeabilized by application of electric pulses. This process,called electropermeabilization or electroporation, allows molecules such as anticancer drugs andnucleic acids to enter into cells and tissues. The knowledge of the mechanisms is mandatory for themethod to be used. Their description takes benefit from studies performed on lipid vesicles, 2D and
3D cells culture. Single cell imaging experiments revealed that the uptake of molecules takes placein well-defined membrane regions. Small molecules can freely cross the electropermeabilisedmembrane. Heavier molecules, such as plasmid DNA, face physical barriers which engender acomplex mechanism of transport.

“Biomedical application of electroporation:
From preclinical research to clinical practice”
Prof. Gregor Sersa

Dpt. Experimental Oncology, Institute of Oncology Ljubljana, Slovenia
gsersa@onko-i.si
Electroporation based biomedical applications are versatile. Irreversible electroporation exploits
irreversible cell damage due to the electroporation of cells. However, reversible electroporation,
does not cause cell death, they recover after electroporation, but it enables drug or gene delivery
to cells due to the increased cell membrane permeability. Electrochemotherapy is the mostadvanced clinically approved therapeutic approach that utilizes electroporation for the intracellularcytotoxic drug delivery to cells. This local ablative technique is used in 140 cancer centersthroughout the Europe for the treatment of various histiotypes of cutaneous tumors, and iscurrently translated also into the treatment of deep seated tumors. Another application ofreversible electroporation is used for naked DNA delivery to cells. This form of gene therapy iscalled electrogene therapy. Its applications for treatment of cancer are in the early beginnings, inthe phase of clinical trials. Especially intriguing is its application in combination withelectrochemotherapy, where adjuvant electrogene therapy with immunomodulatory IL-12 cytokine
to electrochemotherapy could transform local electrochemotherapy to locoregional or systemic
therapy.

“Activation of Immune Cells by Nanosecond Pulsed Electric Fields”
Prof. Ken-ichi Yano
Institute of Pulsed Power Science, Kumamoto University
yanoken@kumamoto-u.ac.jp

Nanosecond pulsed electric fields (nsPEFs) have attracted considerable attention as a novel physicalmeans for life sciences. In this study, we explored the potential of nsPEFs to stimulate specificcellular activities. Using neutrophil-differentiated HL-60 cells, we demonstrated that nsPEFs induce
the formation of neutrophil extracellular traps, a neutrophil-specific immune response.

“Pulsed power for bioelectrics and environmental applications”
Prof. A.J.M. Pemen

Dept. Electrical Engineering, Eindhoven University of Technology, The Netherlands
A.J.M.Pemen@tue.nl
In this contribution we present methods flexible, fast rise-time (<200 ps), nanosecond high-voltage
pulse generation. We focus on the general concepts involved in the design of this pulse source, withspecial attention to the basic underlying principles and the key characteristics that make it operate.
Furthermore, we will discuss a method for the contactless delivery of pulsed electric fields inbioelectrics applications.