Anti-Rad51 (Human) antibody, chicken polyclonal (IgY)
Product code70-005Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified by propriety protocol.

Immunogen

Full-size recombinant human Rad51 protein.(BioAcademia 10-001)

Isotype

Chicken IgYReactivity

Human, Rodents, Chicken, Frog

Special notes

N/A

Application

1. Western blotting (1/1,000~1/5,000 dilution)
2. Immunoprecipitation (1/100-1/1,000)
3. Chromatin immunoprecipitation (assay dependent)
4. Immunofluorescence staining (1/100-1/1,000)* In general, IgY antibody shows significantly less backgrounds than IgG
antibody of mamammals in immuno-reactions

Background

Human Rad51 protein is a functional and structural homolog of E. coli RecA
protein, which plays a major role in genetic recombination and recombination
repair by mediating strand exchange reaction between homologous DNA
strands . Rad51 functionally and physically interacts with its paralogs, Dmc1,Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3, and also with Rad52 inrecombination processes.

It also interacts with oncogenes and tumor

suppressors such as BRACA1, BRACA2, and P53 for the maintenance of genome
stability.

Data Link

UniProtKB/Swiss-Prot Q06609 (RAD51_HUMAN)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 70-005 Anti-Rad51 (Human) antibody, chicken polyclonal (IgY)

Fig.1SDS-PAGE of anti-Rad51 IgY

Non-reduced.

Fig. 2 Western blot analysis of Rad51 in HeLa cell extract
1/2,000 dilution.

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Fig.3. Detection of Rad51 protein foci formation with the anti-Rad51 antibody in X-ray irradiatedhuman fibroblast cells, GM0637.
1) The cells were irradiated with X-ray at 2 Gy, grown for 1 hr, fixed with 4% paraformaldehyde for10 min and washed with PBS three times.
2) Permeabilized by treatment with 0.5% Triton for 5 min and washed with PBS three times.
3) Incubated with the anti-Rad51 antibody at 1/100 dilution or 1/1,000 dilution for 30 min at 37℃
and washed with PBS three times.
4) As the second antibody, Alexa488-conjugated anti-chicken IgY antibody was used at 1/5,000dilution.
5) DNA was stained with Hoechst for 1 min and mounted.

Fig.4 Immunoprecipitation of Rad51 from crude extract of HeLa cells
Rad51 was immunoprecipitated from HeLa cell extract, 20 μg, with anti-Rad51 IgY conjugated toSepharose 4B and the eluate was immunoblotted with rabbit anti-Rad51 antibody (BioAcademia 70012)
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References: This antibody has been used in the following publications.
1. Nakada S. et al. RNF8 regulates assembly of RAD51 at DNA double-strand breaks in the absenceof BRCA1 and 53BP1. Cancer Res. 2012 Oct 1;72(19):4974-83. IF (human)
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