Success Story
IKA® Dispersers and Shakers
An ideal solution for research laboratories!
Aurigene partners with IKA® to enhance its drug discovery and development portfolio
THE CUSTOMER
Specialists in Drug Discovery and Development
Aurigene is a specialized oncology and inflammatory
disease focused drug discovery biotech, with deep target and therapeutic area expertise.
Based in Bangalore and Hyderabad in India and also in
Kuala Lumpur, Malaysia, Aurigene has a fully integrated
drug discovery infrastructure from hit generation to preclinical development. Aurigene‘s focus target areas include oncology and autoimmune/inflammatory diseases.
The company works with established pharma companies by early stage partnerships, licensing and codevelopment, and specialized services in the areas of
crystallography, peptide, small molecule synthesis, nonGMP scale-up and ADME assays.

THE CHALLENGE
To disrupt hard cell walled tissues
Biotechnology is broadly defined as a technique thatuses live organisms viz. bacteria, viruses, fungi, yeast,animal cells, plant cells etc. to make or modify a product, to enhance certain plant or animal traits or to engineer micro-organisms for specific uses.
In this endeavour it is required to isolate the gene,study its function and regulation, modify the gene andreintroduce it into its natural host via another organism
and/or vector system. This process helps facilitate theunlocking of secrets associated with disease resistance,
regulation of growth and development or the communication pathways utilized by cells and among othermicro-organisms.
The main challenge faced when homogenizing tissues is that many organisms, such as seeds and otherheavily encased samples, are not efficiently disrupted
chemically or through the introduction of enzymes. In
these cases, tissue and cell disruption is very difficult
to achieve. Also, the energy needed when using these“harsh methods” is usually high and destroy the very
proteins being sought. Furthermore, using excessive
force is limited because of the generation of detrimental heat and/or shear that can ruin the desired proteins
samples.

Dr Shekar S. Chelur, Associate Research Director from Aurigene has quoted that,®

“IKA products are compact with ergonomic designs andare known for reliable performance. IKA® India provides
good customer support. Homogenizers with disposabledispersion elements offer great advantage to eliminate
cross contamination”

THE ADVANTAGES AT A GLANCE
T 10 basic

VORTEX Genius (VG 3)- Ideal for manual operation-W ide speed range-Lightweight with ergonomic form and direct
mains operation- Special adjustments for microcentrifuge tubes, microtiter plates and Erlenmeyer flasks/conical flasks- 125 W drive allows for speed stability with variousmedia- Suitable for continuous operation with low heatup due to self-ventilation of the motor-Quick release coupling simplifies changing andcleaning of the disperser element- Stainless steel elements with PTFE bearings suitable
for all the methods of sterilization

THE SOLUTION
To produce a uniform homogenate in relatively
less time
With such resilient samples mechanical and physical methods that rely on grinding, shearing, beating and shocking
are used. The most effective method to make a homogeneous mass is still mechanical -rotor/ stator technology.
Tissues that are difficult to break down include heart
muscle, lung, intestine, and skin. The IKA® T 10 basic homogenizer comes with a rotor-stator design with an outerstationary tube (stator) and an inner turning shaft (rotor)
which is connected to a motor. At the bottom of the rotorstator are slots on both the tube and shaft. When running
at 8,000 - 30,000 rpm, samples pressed into the slots of
the rotor-stator are efficiently sheared. The IKA® T 10 basic
is used to macerate animal and plant tissues of increasing
mass. The shearing action of the homogenizers produces
a very uniform homogenate in relatively little time. Sample sizes processed on handheld T 10 basic homogenizer
range from 0.5 ml up to 100 ml.
IKA® offers a range of homogenisers for one to select from- units that can produce a gentle nicking of the cell to release intact organelles in addition to more vigorous disruption to release membrane-bound proteins.
Fragile mammalian cells are broken by moderate shaking (500 – 2500 rpm) of the suspended cells using theIKA® VORTEX Genius 3 (VG 3) shaker. The VG 3 is a simple

device which can be used to mix small vials of liquid or todisperse solids into solution. This unit consists of an electric motor and a drive shaft that is oriented vertically andattached to an inverted rubber cap/ base mounted slightly
off-centre. As the motor runs, the rubber base oscillates
rapidly at 2500 rpm in an orbital motion creating a vortex.
When a test tube or other appropriate container is pressed
into the rubber base (or touched to its edge) the motion
is transmitted to the liquid inside and a vortex is created.
The VG 3 has variable speed settings and can be set torun continuously or to run only when downward pressure
is applied to the rubber base. It is a versatile product thatcan be used with three interchangeable attachments andseven inserts for microcentrifuge tubes, microtiter plates
and 250 ml Erlenmeyer flasks. IKA® VG 3 units also nevershut down even when fully taxed and/or when operating
at full speed during a given procedure.

CUSTOMER BENEFITS
Fully flexible homogenisers with a small footprint and quiet operation
Aurigene has been using the IKA® T 10 basic homogenizers and VG 3 vortex shakers for the past 10 years. They
have more than 20 of these units installed in the laboratory setting. IKA® homogenizers have helped improve theefficiency and homogeneity of the dispersion process. Inaddition, these units have increased productivity by saving time through the reduction of the number of cycles
by which samples are required to be passed in order toachieve the desired homogeneous phase.

IKA® India Private Limited
814/475, Survey No. 129/1, Mysore Road,Kengeri, 560060 Bangalore, Karnataka India
Tel. +91 80 26253900 · Fax +91 80 26253901
Hotline. +91 80 26253925 · info@ika.in · www.ika.in