Anti-ASN (asparagine synthetase)(At) antibody, rabbit polyclonal
Product code81-031

Size

200 µgStorage-20℃

Concentration

4.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from rabbit antiserum.

ImmunogenPurified recombinant Arabidopsis asparagine synthetase isoprotein 2 (ASN2 ),full-size, no-tag attached.

IsotypeRabbit IgG

Reactivity

Plant ASN2 and ASN1 isoproteinsSpecial notes

Validation: The specificity of the antibody has been validated by western
blotting with mutant plants.

Application

1. Western blotting (1/1,000-1/2,000 dilution)
2. Immunohistochemistry, paraffin section (1/100-1/500)
3. ELISA (assay dependent)
Other applications have not been tested.

BackgroundAsparagine synthetase 2 (ASN2) is essential for nitrogen assimilation,
distribution and remobilization within the plant via the phloem. ASN2 isexpressed in leaf and ASN1 is expressed in floral organs.
The amino acid sequences of Arabidopsis ASN1 and ASN2 are 76% identical.
The amino acid sequences of Arabidopsis and Maize ASN2 are 73.6% identical.

Data Link

UniProtKB Q9LV77 (A. thaliana), B5U8J7 (Z. mays)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 81-031 Anti-ASN (asparagine synthetase)( At) antibody, rabbit polyclonal

Fig.1 Western Blot of ASN2 in arabidopsis leaf extract.
Anti-ASN2 antibody was used at 1/1,000 dilution. Secondary antibody (goat anti-rabbit IgG antibody
HRP-conjugated, ab97051) was used at 1/10,000 dilution.
1. Arabidopsis leaf extract, 10 μg
Molecular mass of Arabidopsis ASN2 is 65 kDa.

Fig.2 Absence of ASN2 protein in leaf extracts of asn-2-1 and asn2-2

mutants.

AS is the position of ASN2 protein migrated at 65 kDa in SDS-PAGE and 70 kda is the position ofpre-stained protein size marker. Col0 is wild-type Arabidopsis plant. asn2-1 and asn2-2 are T1
insertion mutants.

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Fig.3 Absence of ASN1 protein in extracts of floral organ of asn1 insertion mutant.
Wild type (Col-0) samples are analyzed in the upper window and asn1 mutant samples are in thelower window. The upper numerals are the protein levels measured by densitometric tracing ofwestern blot. Developmental stages are indicated below. Marker proteins are 72 and 55 kDa.

Fig.4

Immunofluorescence analysis of ASN2 in plant leaf section.

Arabidopsis thin leaf section was subjected to indirect immunofluorescence analysis using the AntiASN antibody as the primary antibody.
Goat anti-rabbit IgG labelled with Alexa 405 (Molecular Probes) was used as a secondary antibody.
Reference: This product has been used in the following publications.
1. Gaufichon L, Masclaux-Daubresse C, Tcherkez G, Reisdorf-Cren M, Sakakibara Y, Hase T,
Clément G, Avice JC, Grandjean O, Marmagne A, Boutet-Mercey S, Azzopardi M, Soulay F,Suzuki A. “Arabidopsis thaliana ASN2 encoding asparagine synthetase is involved in the controlof nitrogen assimilation and export during vegetative growth.” Plant Cell Environ. 2013
Feb;36(2):328-42. PMID: 22789031 WB , IHC ;arabidopsis
2. Gaufichon L, et al. ASN1-encoded asparagine synthetase in floral organs contributes to nitrogen
filling in Arabidopsis seeds. Plant J. 2017 Aug;91(3):371-393. PMID:28390103

WB, IHC;arabidopsis

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