Anti-HEV (Hepatitis E Virus) Capsid Antibody, Rabbit polyclonal
Product code65-093

Size

100 µgStorage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from rabbit antiserum.

Immunogen

Recombinant truncated capsid protein (amino acids 112-608) ofHEV (Genotype 3, 2712 strain)

IsotypeRabbit IgG

Reactivity

Capsid protein of HEVSpecial notes

N/A

Application

1. Western blotting (2 µg/mL)
2. Immunoprecipitation (2 µg/mL)
3. Dot blotting (1 ug/mL)
4. ELISA (assay dependent)

BackgroundHepatitis E virus (HEV) is single-strand positive-sense RNA virus in the family
Hepeviridae. The disease caused by HEV is an important public health problem
in developing countries. A molecular phylogenetic analysis classifies HEV into
four major genotypes (genotype 1-4). The genome HEV consists of about 7200
bases and contains three discontinuous and partially overlapping open reading
frames (ORFs). ORF1 encodes a methyltransferase, protease, helicase andreplicase; ORF2 encodes the capsid protein and ORF3 encodes a protein ofundefined function. The viral capsid protein induces neutralizing antibodies,and contains three subdomains, S (aa112-319), M (aa 320-456) and P (aa 457608). Recombinant capsid protein is composed of approximately 53 kDa, smaller
capsid protein subunit.

Data Link

UniProKB Q6J8F7 (CAPSD_HEVMG), genotype 3

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 65-093 Anti-HEV (Hepatitis E Virus) Capsid Antibody, Rabbit polyclonal

Fig.1 Western blot of recombinant of capsid protein of HEV50 ng of recombinant capsid of HEV was run on SDS-PAGE (12.5% gel) and blotted onto PVDF
membrane for one hour at room temperature (RT).
Anti-HEV capsid antibody was used at 2 µg/ml and incubated for one hour at RT. Second antibody(goat anti-rabbit IgG antibody, HRP-conjugated, ab97051) at 1/10,000 dilution was incubated at onehour at RT.

Fig.2 Immunoprecipitation of recombinant capsid of HEV by anti-HEV capsid antibody
1 µg of recombinant capsid of HEV was immune-precipitated with 10µg of anti-HEV capsid antibody
and the precipitate was immune-blotted with anti-HEV capsid antibody.
Lane 1 : recombinant capsid of HEV
Lane 2 : mock2 / 3

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Fig.3 Dot blotting
Blot of 2µL of anti HEV capsid polyclonal (1/10, 1/100 or 1/1000 diluted serum), HEV infected celllysate or 10ng of recombinant HEV capsid protein.

References for HEV-LP uses for immunization.
1. Yamashita T et al.Biological and immunological characteristics of hepatitis E virus-like particles
based on the crystal structure. PNAS 2009 Aug 4; 106(31):12986-91 . PMID: 19620712. IP, ELISA
2. Li TC et al. Essential elements of the capsid protein for self-assembly into empty virus-likeparticles of hepatitis E virus. J Virol. 2005 Oct;79(20):12999-3006. PMID: 16189002
3. Li TC et al. Protection of cynomolgus monkeys against HEV infection by oral administration ofrecombinant hepatitis E virus-like particles. Vaccine. 2004 Jan 2;22(3-4):370-7. PMID: 14670318
Related Products65-090 Anti-HEV (Hepatitis E Virus) Capsid antibody, mouse monoclonal (161)

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BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/