Founded in 1981, Agri-Analysis LLC is a premierplant pathology laboratory focusing on viral, bacterial and fungal diseases of grapevines. We conductcutting edge research and apply new knowledge anddiscoveries to help clients answer their practicalquestions in grape production. Our customers include growers, growers associations, agriculturalconsultants, universities, research institutes, service
laboratories, seed companies, real estate agents, andproperty owners. We are a CDFA Approved Laboratory under permits No. 2771 and No. 57-11. We arealso under permit by the USDA to receive plant materials originated from outside of the U.S. for testing purposes.

Agri-Analysis LLC
Agri-930 Riverside Parkway, Suite 30
West Sacramento, CA 95605(800)506-9852(800)506-ABOUT US

A declining Zinfandel Vine tested positive for GRBaV.

OUR MISSION
As wine growers are faced with increasing risks andchallenges from important grapevine diseases, AgriAnalysis is your trusted laboratory partner in identifying, managing, and mitigating these risks throughresearch, experience, innovation, cooperation anduncompromising integrity and professionalism.

West Sacramento, CA 95605
Toll Free: (800)506-9852
Fax: (530)757-4655
www.agri-analysis.com

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Agri-Analysis LLC@Agri-Analysis LLC, 2012

We Help Protect Your Vineyards

WHEN WAS GRBaV VIRUS DISCOVERED?

WHAT ARE THE SYMPTOMS TO LOOK FOR?

VINEGUARDSM TESTING SERVICE

During the 17th scientific meeting of the International
Council for the Study of Virus and Virus-Like Diseases of
the Grapevine (ICVG) held at UC Davis, October 7-14,
2012, scientists from Cornell and UC Davis reported thediscovery of a new grapevine virus. Dr. Keith Perry, Department of Plant Pathology of Cornell Univeristy, reported
a new circular DNA virus from grapevine which is named"Grapevine cabernet franc-associated virus (GCFaV)". Dr.
Mysore Sudarshana, USDA-ARS, Department of Plant Pathology of UC Davis reported a circular DNA virus ingrapevines affected by red blotch diseases. They named thisvirus "Grapevine red blotch-associated virus (GRBaV)".
It is believed that GCFaV and GRBaV are the same virusthat belongs to the family of Geminiviridae. Noteworthy isthe fact that it is a circular DNA virus composed of 3,206
nucleotides as opposed to linear RNA viruses which makeup the majority of the grapevine virodom. This is the 2nd
DNA virus found in grapevines.

We have observed typical symptoms ranging from mild
patchy red leaves with red veins to severe vine decline asshown in the cover photo and pictures below:

Our VINEGUARDSM testing service detects the commonly encountered and economically important viraland bacterial pathogens affecting grapevine production. Clients may choose all or part of the pathogens on
this list to test depending on their testing objective.

Symptom on C. Sauvignon Leaf(Light)

Symptom on C. Sauvignon Leaf(Severe)• Grapevine leafroll virus (GLRaV) types 1 through 9@Agri-Analysis LLC, 2012
@Agri-Analysis LLC, 2012

@Agri-Analysis LLC, 2012
@Agri-Analysis LLC, 2012

Symptom on C. Franc Leaf

Symptom on Riesling Leaf

• Grapevine virus A (GVA)• Pierce's Disease (Xylella fastidiosa)• Grapevine fleck virus (GFkV)@Agri-Analysis LLC, 2012

@Agri-Analysis@Agri-Analysis
LLC,LLC,2012 2012

WHAT MAKES GRBaV VIRUS IMPORTANT?

We have found this virus in samples from Napa, Sonoma,
Paso Robles, San Luis Obispo, Monterey, Mendocino,Santa Barbara areas as well as in states of Virginia andMaryland. Grape varieties include not only red such as
Merlot, Zinfandel, Mourvedre, Petite Syrah, Cabernet
Franc, Cabernet sauvignon, Malbec, Pinot Noir, Petit Verdot, but also white varieties such as Chardonnay and Riesling. The virus appears to be widely spread in Californiaand other wine growing regions of the U.S.

Although newly found in grapevines, members of thegeminiviridae family have been known to cause serious
damages in other crops. For example, it was reported inearly 1990s that geminiviruses destroyed up to 95% of thetomato harvest in the Dominican Republic. In the 199192 growing season in Florida, they caused $140 million indamage to the tomato crop there. While there is much we
do not know about the impact of this virus in grapevines,
preliminary indications suggest that it could cause potentially serious damages including vine decline, delayed
ripening, low yield, and low sugar content.

In other crops, viruses of the geminiviridae family are
known to be transmitted by whiteflies and leafhoppers feeding on plant phloem fluids. Some research suggests that theglobal climate change is causing the virus to migrate fromtropical climate to new areas. However, it is too early to tellexactly what vectors harbor and transmit this virus in grapevines. Therefore, pest management guidelines are not yet
available. New and exciting research activities are bound toanswer these questions in the near future.

• Grapevine virus B (corky bark virus)• Grapevine virus D (GVD)

WHERE HAS THE RED BLOTCH VIRUS BEEN
FOUND & IN WHAT VARIETIES?

HOW IS THE GRBaV VIRUS TRANSMITTED?

• Grapevine fanleaf virus (GFLV)• Rupestris stem-pitting associated virus• GLRaV-2 Red Globe Strain (GLRaV2-RG)• Grapevine Red Blotch Associated Virus(GRBaV)

Sample Collection Protocol

HOW IS THE GRBaV VIRUS DETECTED?
Currently only one isolate of GRBaV has been reported.
We use state-of-the-art polymerase chain reaction technology to detect this virus. We also use DNA sequencing
which is the gold standard for identification. As more genetic variants are discovered, we constantly develop new
detection methods to improve sensitivity and specificity.
We collaborate with scientists from two of the world’s
leading universities in their research efforts so as to enhance our understanding and detection capability.

We Help Protect Your Vineyards.