Anti-SUMO1 antibody, rat monoclonal (4D12) (FITC)
Product code

70-655

Size

50 µg

Storage-20℃

Concentration

1.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from hybridoma cell culture medium.

ImmunogenPurified recombinant GST-fused human SUMO1 (full length)

IsotypeRat IgG 2aκ

Reactivity

Specific to human, simian, mouse and rat SUMO1.
Other species have not been tested.

Special notes

Conjugation: FITC

Application

1. Immunofluorescence staining (1/100 dilution)
2. Immunohistochemistry, frozen section (1/100 dilution)

Background

SUMO (Small Ubiquitin-like Modifier) proteins are a family of small proteinsthat are covalently attached to and detached from other proteins in cells tomodify their function. Unlike ubiquitination, which targets proteins for
degradation, SUMO modification plays a critical role in a number of cellularfunctions including nucleocytoplasmic transport, gene expression, cell cycle andformation of subnuclear structures such as promyelocytic leukemia (PML)bodies. There are three confirmed SUMO isoforms in human; SUMO1, SUMO2
and SUMO3. SUMO2 /3 show a high degree of similarity to each other and are
distinct from SUMO-1. Individual SUMO family members are all targeted todifferent proteins with diverse biological functions. SUMO-1 is conjugated toRanGAP, PML, p53 and IκB-α to regulate nuclear trafficking, formation ofsubnuclear structures, regulation of transcriptional activity and protein
stability. SUMO1 is encoded as a 101 aa protein and first Met and C-terminal
4 aa are removed from the preprotein.

Data Link

Swiss-Prot P63165 (human)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
1 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 70-655 Anti-SUMO1 antibody, rat monoclonal (4D12) (FITC)

Fig.1. Immunofluorescence staining of SUMO-1 with the antibody 4D12 in the mouse primary culture
neurons.
Left: Stained with anti-SUMO-1 antibody 4D12 at 10 μg/ml.
Light: DNA was stained with Hoechst

Fig.2. SUMO-1 colocalizes with SUMO-2/3 as revealed by indirect immunofluorescence staining of C33A cells (human cervix carcinoma).
Left: SUMO-1 was stained with anti-SUMO-1 antibody (4D12) at 10 μg/ml
Middle: SUMO-2/3 was stained with anti-SUMO-2/3 antibody (3H12).
Right: Merged image

Fig.3 Fluorescence immunocytochemistry for endogenous SUMO1 expression.
HEK293A cells were fixed, permeabilized, and stained with SUMO1-FITC (1: 50) antibody andHoechst 33342. Scale bar, 50 mm.
2 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

References: This antibody was used in Ref. 3 and 4.
1. Ulrich HD "The fast-growing business of SUMO chains.” Review Mol Cell 32: 301–305 (2008)
PMID: 18995828
2. Cheng J et al “Role of desumoylation in the development of prostate cancer.” Review Neoplasia 8:667-676 (2006) PMID: 16925949
3. Uchimura Y et al “Involvement of SUMO modification in MBD1- and MCAF1-mediatedheterochromatin formation.” J Biol Chem 281: 23180-23190 (2006) PMID: 16757475
4. Saitoh N et al “In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear
rim and PML bodies.” Exp Cell Res 312: 1418-1430 (2006) PMID: 16688858

3 / 3

BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/