Pasteurella multocida Toxin (Active)
01-50750μg

Storage: Ship at 4℃ or －20℃ and store at －20℃
（or -80℃ for long period）.
Applications:1)

For the studies on the GTP trimer bound protein- dependent signaling pathway

2)

As a PMT antigen for immunological assays such as ELISA, western blotting and dot blotting.

Form: 50% glycerol, 5mM Tris-HCl(pH7.5), 0.1M NaCl
Purity:More than 90% purity by SDS-PAGE (CBB staining)

Protein Concentration: 254 μｇ/ml
Biological Activity Assayed: See Figures below. *Research use only, not for human use.
Background: Pasteurella multocida toxin (PMT) is produced by a gram-negative bacillus, Pasteurella

multocida. PMT activates the Gq and G12/13 dependent signaling pathways. Gq and G12/13 are alphasubunits of the GTP trimer bound protein of animal cells.
G11-dependent pathways.

It does not activate the highly relatedThis toxin binds to a ganglioside-type cell surface receptor, acts

intracellularly after having been internalized through an endocytic pathway, and has pleiotropic
effects on cell physiology. Therefore it does not function on receptor-deficient cells or cells defective inthe endocytosis pathway.
PMT is encoded by the toxA gene of P. multocida.
with His6-tag at N-termina expressed in E. coli.

This product is derived from a recombinant ToxA

Its molecular weight is 145 kDa (Fig.1)*Research use only, not for human use
Data Link

UniProtKB/Swiss-Prot P17452 (TOXA_PASMU)

References:
1.

Orth JH et al “Pasteurella multocida toxin-induced
activation of RhoA is mediated via two families of G{alpha}proteins, G{alpha}q and G{alpha}12/13.”J.BiolChem
280:36701-36707(2005) PMID: 16141214

2.

Wilson BA and Ho M “Pasteurella multocida toxin asa tool for studying Gq signal transduction.”Rev Physiol

Biochem Pharmocol 152:93-109(2004) PMID: 15455260

Fig.1 SDS-polyacrylamide gel electrophoresis of PMT
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Fig. 2. Assay of Biological activity of PMT:
Mouse embryonic cells (Swiss 3T3) were treated with PMT at 10 ng/ml at 37℃ for 18 h.
Left; untreated cells.

Right, Treated cells; Foci formation by aggregationof cells was observed.

MATERIAL SAFETY DATA SHEET

Hazardous Ingredients: Recombinant Pasteurella multocida toxin with His6-tag(MGH6DYDIPTTENLYFQGAHMGIQ )attached to N-terminal of the toxin.(first methionin removed).
Physical Properties；The material is provided as liquid solution in 50% glycerol, 5mM Tris-HCl (pH7.5),
and 0.1M NaCl
Fire and Explosion Hazard Data: Not applicable
Health Hazard:
The LD50 in rats is 0.5 μg/kg when injected parenterally (Cheville, N.F. and Rimler, R.B.,

Vet. Pathol. 26: 148-157, 1989). Toxicity data for humans is not available.
Target organ(s): Liver and.Lungs. If topical contact occurs, flush with copious amounts of water. Forinternal exposure, consult a physician.
Spill or Leak Procedures:
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If a spill occurs, cover with a damp cloth or paper towel. Wipe up and autoclave thismaterial. Further, clean the area with 5% bleach. Solutions may be inactivated byautoclaving at 121℃ and 15 psi for 15 minutes or by heating to 56°C for 30 minutes.
Special Protection Information:
Wear safety glasses, protective clothing, and rubber or latex gloves. and when handling hypodermic
needles avoid inadvertent selfinoculation. Do not pipette by mouth. Avoid inhalation of this product.
Special Procedures:This product is to be used by skilled personnel in a laboratory setting only. Goodlaboratory technique should be employed. This product is for research purposes only. It
is not for use in humans and is not to be used as a diagnostic agent
Contact to Producer:
BioAcademai Inc. 7-7-18 Saito-Asagi, Ibaraki, Osaka 567-0085
Tel. 81-72-643-4660

Fax. 81-72-643-4660

E-mail: info@bioacademia.co.jp

BioAcademia,Inc.

Tel. 81-6-6877-2335

Fax. 81-6-6877-2336

info@bioacademia.co.jp

http://www.bioacademia.co.jp/en/