DNA (cytosine-5)-methyltransferase 1 (Dnmt1) (mouse), Functional
The Cartagene protocol on Biosafety: Applicable
Product code

10-201Size

300units

Storage

Store at -80℃. Avoid freeze-thaw cycles.

ProductRecombinant mouse Dnmt1 (aa 291-1620), His-Tag attached to N-termial,
expressed in baculovirus expression system.

Concentration

0.5 mg/ml

Buffer

0.2M NaCl, 10mM HEPES (pH 7.4), 50% glycerol

PurityGreater than 95% protein determined by SDS-PAGE (CBB staining) (Fig.1)

Reagents#10-Drb

5 x Dnmt1 Reaction Buffer (1.5ml）

Supplied with#10-Sam

S-adenosylmethionine (SAM) (10~20mM) which was purified byEnzyme

chromatography from the commercial reagent and dissolved in H2O.
（Lot01 20mM 20µl）(Lot02 16.2mM 25µl)
Note: SAM is very unstable. Store at -80℃

Application

1. In vitro methylation of cytosine residues in hemimethylated DNA at
5’….CG…3’.
2. Antigen for anti-mammalian Dnmt1 antibodies.

Specific activity

Definition of specific activity:
1 unit is defined as the amount of the enzyme that transfer 1 pmole of methylgroup to poly dI-dC substrate during 30 minutes at 37℃Specific activity: 17 units/µl

BackgroundDNA methylation is significant for epigenetic regulation of gene expression, X
chromosome inactivation, genomic imprinting, and development.

Abberant

methylation patterns are associated with certain human tumors anddevelopmental abnormalities.

In vertebrates, there are two types of DNA

methyltransferase activities; de novo and maintenance types.

Two DNA

methyltransferases, Dnmt3a and Dnmt3b, are responsible for the creation ofmethylation patterns at an early stage of embryogenesis (de novo-type), while
Dnmt1 is responsible for the maintenance of methylation patterns during
replication.

Dnmt1 favors to methylate the hemimethylated DNA andpreferentially methylates one strand of the double-stranded DNA during its
processive methylation. This product, mouse Dnmt1 deleting the N-terminal
290 amino acid residues, was expressed using a baculovirus expression system*
Data Link

UniProt P13864 (DNMT1_MOUSE)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/

Data Images: 10-201 DNA (cytosine-5) methyltransferase 1 (Dnmt1) (mouse), Functional

Fig.1 SDS-polyacrylamide gel electrophoresis of recombinant Dnmt1
Related products:
70-201 anti-Dnmt1 (1-248) antibody, rabbit polyclonal
70-206 anti-Dnmt3b (mouse) antibody, rabbit polyclonal
References:This product was described in ref.1 and used in the subsequent publications.
1.Vilkaitis G et al. Processive methylation of hemimethylated CpG sites by mouse Dnmt1 DNA
methyltransferase. J Biol Chem. 2005 Jan 7;280(1):64-72. PMID:15509558
2.Ross JP et al. Recombinant mammalian DNA methyltransferase activity on model transcriptional
gene silencing short RNA-DNA heteroduplex substrates. Biochem J. 2010 Dec 1;432(2):323-32.
PMID: 20846120
3.Takeshita K et al. Structural insight into maintenance methylation by mouse DNA
methyltransferase 1 (Dnmt1). Proc Natl Acad Sci U S A. 2011 May 31;108(22):9055-9. PMID:
21518897
4.Takahashi S et al. A novel method to analyze 5-hydroxymethylcytosine in CpG sequences using
maintenance DNA methyltransferase, DNMT1. FEBS Open Bio. 2015 Sep 8;5:741-7. PMID:26504739

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BioAcademia,Inc. Tel. 81-6-6877-2335

Fax. 81-6-6877-2336 info@bioacademia.co.jp https://www.bioacademia.co.jp/en/