“Flow cytometry is thestate-of-the-art methodin the breeding industry
and in research for
determining both theploidy level and genome
size in plants, animals
and microorganisms.

”

Based on the proof of concept of its predecessors in industry
and research, Sysmex Partec is proud to offer you anup-to-date flow cytometry solution with its third-generation
CyFlow Ploidy Analyser (CyFlow PA).
Genome size determination requires stoichio­metric
DNA labelling and lowest coefficients of variation in DNA
quantification. The CyFlow PA uses a 532 nm laser andthe DNA fluorochrome propidium iodide, which produces
superior results for genome size analysis compared tostandard flow cytometers using lasers of 488 nm.
Due to its superb high-resolution DNA histograms and its
ease of use, the fluorescent dye DAPI is the most power
ful, fast and economic solution for analysing ploidy leveland detecting aneuploidy. Sysmex Partec’s unique UV LED(365 nm) is the optimal light source to fully exploit thisfluorochrome’s features.

Zea mays, 2n – diploid, CyStain DAPI staining

Zea mays, 4n – tetraploid, CyStain DAPI staining

The CyFlow PA provides ploidy and genome size analysis in less than two minutes. The instrument is available in the threefollowing versions: for the analysis of samples stained with (1) DAPI, (2) PI or (3) DAPI and PI.

Technical specifications
Instrument models and light sources
ModelLight source(s)CyFlow Ploidy Analyser DAPIUV LED (365 nm)

CyFlow Ploidy Analyser PI

green laser (532 nm, 30 mW)

CyFlow Ploidy Analyser DAPI + PI

UV LED (365 nm)

Optics1 or 2 optical parameters with selected photomultiplier tubes (PMT)Standard set-up and filters for propidium iodide (PI) and/or DAPI/SSC

Flow system

Quartz flow cuvette for laminar sample transport and hydrodynamic focusing
Sample port with biosafety cleaning function
True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
Computer-controlled syringe pump speed, adjustable from 0 – 20 μL/s
Fluid and waste reservoirs with fluid level sensors

Electronics and signal processing

Selectable linear or 4-decade logarithmic scale
16-bit analogue-to-digital converters, selectable trigger parameter and individual
threshold level settings

SoftwareOperating system: Microsoft Windows™
Sysmex Partec operating software for real-time data acquisition, display, analysis and reporting
Data format: flow cytometry standard (FCS)

Computer system

Integrated Microsoft Windows™ PC with Microsoft Office®
Integrated, foldable 15" colour LCD TFT display
Ethernet and USB ports
DeskJet colour printerOptional external screen (dual screen mode)

Weight18 kg

Dimensions (W x H x D)

385 x 290 x 280 mm; with open display 528 mm height

Sysmex Asia Pacific Pte Ltd
Tel +65 6221-3629 Fax +65 6221-3687

Sysmex (Malaysia) Sdn Bhd
Tel +60 (3) 5637-1788 Fax +60 (3) 5637-1688

Sysmex Vietnam Co., Ltd
Tel +84 (8) 3997-9400 Fax +84 (8) 3997-9405

Sysmex India Pvt. Ltd
Tel +91 (22) 6112-6666 Fax +91 (22) 2577-6790

Sysmex (Thailand) Co., Ltd
Tel +66 (2) 032-2536 Fax +66 (2) 116-5396

Sysmex Vietnam Co., Ltd (Hanoi Branch)
Tel +84 (4) 3776-7020 Fax +84 (4) 3776-7022

PT Sysmex Indonesia
Tel +62 (21) 3002-6688 Fax +62 (21) 3002-6699

Sysmex Philippines Inc.
Tel +63 (2) 621-2460 Fax +63 (2) 621-2432

Sysmex Australia Pty Ltd
Tel +61 (2) 9016-3040

www.sysmex-ap.com

www.sysmex.co.inwww.sysmex.co.id

www.sysmex.com.my

www.sysmex.co.th

www.sysmex.com.ph

Design and specifications may be subject to change without prior notice due to further product development.

green laser (532 nm, 30 mW)

www.sysmex.com.vn

www.sysmex.com.vn

www.sysmex.com.au

Sysmex New Zealand Ltd
Tel +64 (9) 630-3554

www.sysmex.co.nz

SAP/FlowCytometry/CyFlowPloidy/17042020/V2.0

Flow Cytometer for High-resolution DNA analysis

CyFlow Ploidy Analyser
For Agroscience,breeding andaquaculturewww.sysmex-ap.com

A dedicated solution for ploidy analysis
and genome size determination
Determining ploidy is a particularly important form of analysis in plant breeding and aquaculture: controlling
the ploidy level is often essential for monitoring the outcome of breeding procedures and quality in seed andplant production.
As such, accurately determining genome size and ploidy levels plays a major role in today’s evolutionary
biology, taxonomy and ecology. It helps to characterise and understand how species develop and the detailsof population structures.
Counting chromosomes by classical light microscopy has been
replaced by flow cytometry, i.e. determining fluorescently labelled
nuclei and their analysis in a flow cytometer. This method is timeand cost-saving as it provides precise results quickly with anefficient and validated workflow.

CyFlow Ploidy Analyser

Example of a ploidy analysis report

The CyView software integrates instrument control and complete
data analysis for ploidy and genome size measurements. It combines
a 1-parameter DNA histogram display for genome size and ploidy
determination with a 2-parameter dot-plot display (64 – 4,096 channels)
to gate and separate intact and fragmented nuclei.

CyView features two automatic peak-finding algorithms
and man-ual peak definition. The software also lets you createuser-defined peak-finding procedures to optimise this functionin terms of the requirements of specific experiments. Resultsof data analysis are clearly represented on screen and can bedirectly exported together with the related DNA histograms
to MS Excel worksheets for further analysis.

CYFLOWPLOIDY
CyFlow Ploidy

Optimal sample preparation
We offer a range of different Sysmex Partec reagent kits toprepare your samples for flow cytometry analysis on yourCyFlow PA. The reagent kits include ready-to-use staining
solutions and nuclei extraction for analysing absolute or
relative genome size variation and ploidy level of plant
cells and cells of different origin (e.g. leaf and root tissue,seed, in vitro material). The staining can be applied tovarious biological tissue specimens.
Order-No.

Reagents

Dye05-5001

CyStain UV Ploidy

DAPI05-5002

CyStain UV Precise PDAPI05-5003

CyStain UV Precise T

DAPI

05-5004

CyStain DNA 1 step

DAPI

05-5005

CyStain DNA 2 steps

DAPI

05-5007

CyStain UV OxProtect

DAPI05-5022

CyStain PI Absolute P

PI05-5023

CyStain PI Absolute TPI

05-5027

CyStain PI OxProtect

PI

CyStain PI Absolute T reagent kit