Anti-VSR1 (At) antibody, rabbit polyclonal
Product code81-119Size

100 µgStorage-20℃

Concentration

2.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from rabbit antiserum.

Immunogen

Recombinant His6-VSR1 (amino acids 20-566) of Arabidosis thaliana.

IsotypeRabbit IgG

Reactivity

Arabidopsis thaliana and Nicotiana tabacum.

Special notes

N/A

Application

1. Western blotting (1/1,000-1/5,000)
2 .Immunofluorescent staining (1/500)

Background

Vacuolar-sorting receptor (VSR) involved in clathrin-coated vesicles sortingfrom Golgi apparatus to vacuoles. Required for the sorting of 12S globulin, 2S
albumin and maybe other seed storage proteins to protein storage vacuoles(PSVs) in seeds. May also be implicated in targeting N-terminal propeptide
containing proteins to lytic vacuoles. 623 amino acids with mass 68,992.
Subcellular localization: Golgi apparatus membrane.

Data Link

UniProtKB P93026 (VSR1_ARATH)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 81-119 Anti-VSR1 (At) antibody, rabbit polyclonal

Fig.1 Western blot of dVPE in extract of maturing siliques of arabidopsis
Crude extract of 19-day-old seedlings of Arabidopsis thaliana was run on SDS-PAGE (12.5% gel) andblotted to PVDF membrane by wet system. Blocking was done with 3% skim milk. The anti-VSR1
antibody was used at 2 μg/ml. Secondary antibody (goat anti-rabbit IgG antibody HRP-conjugated,ab97051) was used at 1/10,000 dilution.
Reference. This antibody was described in Ref.1 and used in the following publications.
1. Yamada K et al. Endosomal proteases facilitate the fusion of endosomes with vacuoles at the final
step of the endocytotic pathway. Plant J. 2005 Mar;41(6):888-98. PMID: 15743452.IF (Nicotianatabacum)
2. Tamura K et al. Arabidopsis KAM2/GRV2 is required for proper endosome formation andfunctions in vacuolar sorting and determination of the embryo growth axis. Plant Cell. 2007Jan;19(1):320-32. PMID: 17259264.WB (arabidopsis)
3. Fuji K et al. Arabidopsis vacuolar sorting mutants (green fluorescent seed) can be identified
efficiently by secretion of vacuole-targeted green fluorescent protein in their seeds. Plant
Cell. 2007 Feb;19(2):597-609. PMID: 17293568 WB (arabidopsis)
4. Yamazaki M et al. Arabidopsis VPS35, a retromer component, is required for vacuolar protein
sorting and involved in plant growth and leaf senescence. Plant Cell Physiol. 2008 Feb;49(2):14256. PMID: 18222962 WB (arabidopsis)
5. Kang H et al. Trafficking of vacuolar proteins: the crucial role of Arabidopsis vacuolar protein
sorting 29 in recycling vacuolar sorting receptor. Plant Cell. 2012 Dec;24(12):5058-73. PMID:
23263768.WB (arabidopsis)
2 / 3

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6. Kunieda T et al. Spatiotemporal secretion of PEROXIDASE36 is required for seed coat mucilage
extrusion in Arabidopsis. Plant Cell. 2013 Apr;25(4):1355-67. PMID: 23572548. WB (arabidopsis)
7. Ichino T et al.GFS9/TT9 contributes to intracellular membrane trafficking and flavonoid
accumulation in Arabidopsis thaliana. Plant J. 2014 Nov;80(3):410-23. PMID: 25116949.WB(arabidopsis)
8. Fuji K et al. The Adaptor Complex AP-4 Regulates Vacuolar Protein Sorting at the trans-Golgi
Network by Interacting with VACUOLAR SORTING RECEPTOR1. Plant Physiol. 2016
Jan;170(1):211-9. PMID: 26546666.WB (Arabidopsis)
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