Anti-Nucleobindin 2/ NEFA / Nesfatin precursor antibody, rabbit polyclonal
Product code73-109Size

100 µgStorage-20℃

Concentration

2.0 mg/ml

Buffer

PBS- with 50% glycerol

PurityPurified IgG fraction with protein A from rabbit antiserum.

ImmunogenPurified recombinant GST-fused mouse nucleobindin 2 (aa 26-420)

IsotypeRabbit IgG

Reactivity

Mouse rat and human nucleobindin 2.

Special notes

N/A

Application

1. Western blotting (1/1,000-1/3,000).
2. Immuno-precipitation. (1/200)
3. Immuno-cytochemistry (1/300-1/1,000)
4. Immuno-histochemistry (1/300-1/1,000)
5. Immuno-electron microscopy (assay dependent)
6. Immuno-affinity chromatography (assay dependent)

Background

Nucleobindin 2 (NUCB2), also known as NEFA or Nesfatin precursor, is aubiquitously expressed EF-hand Ca2+ binding protein that is implicated invarious physiological processes. Nucleobindin 2 interacts with the postmitotic
growth suppressor necdin in neurons. Both necdin and nucleobindin 2 areexpressed in differentiated neurons and skeketal muscles and these proteins arelikely to be involved in the regulation of survival and death of postmitotic cellsby controlling Ca2+ homeostasis.

Data Link

UniProtKB P81117 (mouse), Q9JI85 (rat), P80303 (human)

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES. NOT FOR MILITARY USE.
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Data Images: 73-109 Anti-Nucleobindin 2/ NEFA / Nesfatin precursor antibody, rabbit polyclonal

Fig.1 Western blot of tissue extract of mouse cerebral cortex with anti-nucleobinding 2 antibody(NET1).
Extract 10 μg protein was used. Antibody was used at 1/1,000 dilution.
The apparent molecular mass (55~57 kDa) is larger than the calculated one (50 kDa) and the band isbroad, which may reflect post-translational modifications (one glycosylation and five phosphorylationsites)

Fig.2 Expression of Nucleobindin-2 in various tissues as examined by western blotting.
Distribution of nucleobindin 2 in neonatal mouse organs. Homogenates of various organs from P0mouse were separated by 10% SDS-PAGE and immunoblotted with this antibody. The antibody wasused at 1/1,000 dilution.

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Fig.3. Immunocytochemistry for endogenous nucleobindin 2Cells were stained with this antibody by the avidin-biotin-peroxidase complex method.
Left panel; undifferentiated murine embryonal carcinoma P19 cells (UD). Right panels, enriched postmitotic neurons (PN). Nucleobindin 2 was localized to the cytoplasm near the nucleus in
undifferentiated P19 cells, and its immunoreactivity in the cytoplasm was increased when P19 cells
were induced to differentiate into neurons.

Fig.4 Immunohistochemistry for nucleobindin 2 in neonatal mouse brain with this antibody
Frozen brain sections from neonatal mouse were stained with this antibody by the avidin-biotin
peroxidase complex method. A-C, cerebral cortex (parietal lobe). At higher magnification (B, C), finegranular immunoreactive materials are observed at both neuronal dendrites (arrows) and perikarya(asterisks) in the layer IV (arrowhead B in A) and subplate (arrowhead C in A) of the cerebral cortex.
Scale bars, 100 um (A) and 10um (B and C).
Reference: This antibody was described and used in the following publication.
1. Taniguchi N et al (2000) “The postmitotic growth suppressor necdin interacts with a calciumbinding protein (NEFA) in neuronal cytoplasm.” J Biol Chem 275: 31674-31681 PMID: 10915798
WB, IP, IF, IHC, Immuno-affinity chromatography
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