Challenge in the field of
regenerative medicine
Katsuya Hisamichi MD. FAAD.
Corporate Officer,
Research & Development
ROHTO pharmaceuticalRegenerative medicine
Tissue engineering

Transplantation

Stem cells
Induction

Injection
Stem Cells : Can self-renew
 Can differentiate
 Are undifferentiated2Cultivation

Cell therapy

Why MSCs?
3 excellent features !!
 Safty Very Low
Immunogenicity
 Very Low
Tumorgenicity

 TherapeuticEfficacy
 Homing Effect Trophic Effect Suppressinflammation

 Easy Manufacturing
 Easy to obtain Easy to culture
3Stem cells are attracting the mostattention now◎Our strategy

ADSC

 Serum free Animal Origin Free
 High purity stem

【Advantage】【Character】

Easily obtainable fromadipose
Low risk of rejection
Low risk of tumorigenicity
Free from genetic
engineeringImmunosuppressiveHoming effectEasy to cultivate
Injectable

Rohto focuses on the ADSCs (Adipose-derived stem cells)
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Cell therapy

Target Diseases of Cell Therapy
Liver

Diabetes and its complications

Liver cirrhosis
Pulmonary
fibrosis

Malignancy
Blood cancer
Bladder cancer
Prostate cancer
Ovarian cancerSkin
Skin wound
Hair loss

Diabetes
Nephropathy

Neuropathy
Retinopathy

Immunology
Autoimmune disorder
Crohn’s disease
GvHD5Neurology
Parkinson's disease
Spinal cord injury
Amyotrophic lateral sclerosis

CardiovascularHeart failure
Buerger disease
Blood cancer

MSC Teherapy in Diabetes
Suppress pro inflammatory
cytokines and prevent β-cell
apoptosis

TNFαReduce Insulin resistance factors in key
organs IRS-1, AKT/PKB, GLUT4･･･and
improve insulin sensitivity

InsulinInsulinInsulinMuscleLiver
6IFN-γAdiposeDiabetes: facts and figures• The number of people with type 2 diabetes isincreasing in every country• 77% of people with diabetes live in low- andmiddle-income countries• The greatest number of people with diabetes
are between 40 and 59 years of age• 179 million people with diabetes
are undiagnosed(IDF in 2014)7

Diabetes: facts and figures• Diabetes caused at least USD 612 billion
dollars in health expenditure in 2014 – 11% of
total spending on adults• More than 21 million live births were affectedby diabetes during pregnancy in 2013
• In the Middle East and North Africa, 1 in 10
adults has diabetes• In South-East Asia, almost half of people withdiabetes are undiagnosed(IDF in 2014)8

Diabetic patients in the world

Source: IDF Diabetes Atlas Sixth Edition, International Diabetes Federation 2013

Diabetic foot ulcer

10Mass production in Cell Processing
Center (CPC)
May 2014; CPC Validation
June 2014;◎CPC Design

Single-use manufacturing equipment
for cell culture medium
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Cell Culture Kit Currently
Under Development★On sale soon

ROHTO Cell Culture Kit①Medium
Animal Origin Free
Serum Free
GMP* manufacturing
Virus Free
②Human Adipose-derived Stem Cell
Homogeneous Japanese origin
GMP* manufacturing
Virus Free (19 types of virus)
Research use only

GMP*:Good Manufacturing Practice24

Would you join our research?
If you are interested, we’ll show you our facilities.

KyotoTokyoThe Institute of Medical Science
The University of Tokyo Rohto’s Research Laboratory

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Rohto Tokyo Laboratory

 Cell Processing Center

Rohto Research Village Kyoto
 Research Laboratory
 Automated cell culture
systemThank you
Rohto Pharmaceutical Co., Ltd.

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Rohto Pharmaceutical Co., Ltd., 2013, All Rights Reserved