JRD Vol. 70 (2)https://www.jstage.jst.go.jp/browse/jrd/70/2/_contents/-char/en
https://pubmed.ncbi.nlm.nih.gov/38569840/
https://www.jstage.jst.go.jp/article/jrd/70/2/70_2024-E01/_pdf/-char/en
Obituary (Special thanks)
In memory of Dr. Ryuzo Yanagimachi (Yana) (1928–2023)
Teruhiko WAKAYAMA, Atsuo OGURA
Volume 70 Issue 2 Pages i-iv
DOI https://doi.org/10.1262/jrd.2024-E01
Original Article
Identification and characterization of dystrophin-locus-derived testis-specific protein: A testis-specific gene within
the intronic region of the rat dystrophin gene
Keitaro YAMANOUCHI, Shizuka KATO, Yukie TANAKA, Masanari IKEDA, Yukina OSHIMO, Takanori SHIGA,Kei HATAMOTO, James CHAMBERS, Takuya IMAMURA, Ryuji HIRAMATSU, Kazuyuki UCHIDA, Fuko
MATSUDA, Takashi MATSUWAKI, Tetsuya KOHSAKA
Volume 70 Issue 2 Pages 55-64
DOI https://doi.org/10.1262/jrd.2023-073
Effect of paternal aging and vitrification on mitochondrial DNA copy number and telomere length of mouse
blastocysts
Nao ABURADA, Jun ITO, Yuki INOUE, Taiyo YAMAMOTO, Masamune HAYASHI, Noko TERAMOTO, Yuri
OKADA, Yuichi KOSHIISHI, Koumei SHIRASUNA, Hisataka IWATA
Volume 70 Issue 2 Pages 65-71
DOI https://doi.org/10.1262/jrd.2023-079
Lipid droplets synthesized during luteinization are degraded after pregnancy
Junichiro MITSUI, Megumi IBAYASHI, Ryutaro AIZAWA, Tomonori ISHIKAWA, Naoyuki MIYASAKA, Satoshi
TSUKAMOTO
Volume 70 Issue 2 Pages 72-81
DOI https://doi.org/10.1262/jrd.2023-095
Efficient derivation of embryonic stem cells and primordial germ cell-like cells in cattle
Atsushi SHIRASAWA, Masafumi HAYASHI, Mayumi SHONO, Atsushi IDETA, Takashi YOSHINO, Katsuhiko
HAYASHI
Volume 70 Issue 2 Pages 82-95
DOI https://doi.org/10.1262/jrd.2023-087
Cover Story:
The process of inducing the germ cell lineage from pluripotent stem cells, referred to as in vitro gametogenesis, aids
in comprehending the mechanisms involved in germ cell differentiation and offers an alternative source of gametes
for reproduction. Shirasawa et al. have developed a novel method for robust induction of primordial germ cell-likecells (PGCLCs) from newly established bovine embryonic stem (bES) cells (Shirasawa et al.: Efficient derivation ofembryonic stem cells and primordial germ cell-like cells in cattle, pp. 82–95). After a 24-hour culture with bonemorphogenetic protein 4 (BMP4), followed by a three-dimensional culture with BMP4 and chemicals modulating
WNT signaling, bES cells exhibited positive expression for a set of primordial germ cell (PGC) markers, including
PRDM1/BLIMP1, TFAP2C, and SOX17. These outcomes are anticipated to have practical implications for thedevelopment of stem cell-based reproductive technologies in cattle.

Progesterone and estradiol regulate sperm hyperactivation and in vitro fertilization success in mice
Miyu FUJIKURA, Masakatsu FUJINOKI
Volume 70 Issue 2 Pages 96-103
DOI https://doi.org/10.1262/jrd.2023-080
Busulfan administration replicated the characteristics of the epididymal initial segment observed in mice lacking
testis-epididymis lumicrine signaling
Daiji KIYOZUMIVolume 70 Issue 2 Pages 104-114
DOI https://doi.org/10.1262/jrd.2023-102
Parallel expression patterns of NR4A nuclear receptor family genes in the pituitary gland of proestrus rats
Ryota TERASHIMA, Daiki NAGAO, Masato IKEO, Keisuke MORIOKA, Titaree LAOHARATCHATATHANIN,
Shiro KURUSU, Mitsumori KAWAMINAMI
Volume 70 Issue 2 Pages 115-122
DOI https://doi.org/10.1262/jrd.2023-090
Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes
Naomi NAKAGATA, Satohiro NAKAO, Nobuyuki MIKODA, Katsuma YAMAGA, Toru TAKEO
Volume 70 Issue 2 Pages 123-130
DOI https://doi.org/10.1262/jrd.2023-067
High-concentration bovine serum albumin enhances fertilization ability of cold-stored rat sperm
Katsuma YAMAGA, Satohiro NAKAO, Nobuyuki MIKODA, Jorge Mario SZTEIN, Naomi NAKAGATA, Toru
TAKEO
Volume 70 Issue 2 Pages 131-137
DOI https://doi.org/10.1262/jrd.2023-085
The fertility of dairy heifers and cows is not influenced by the follicular wave of the ovulatory follicle
Javad MOHAMMADI, Mehdi AZARI, Mojtaba KAFI
Volume 70 Issue 2 Pages 138-143
DOI https://doi.org/10.1262/jrd.2023-084-----------------------------------------------------JRD Vol. 70 (1)https://www.jstage.jst.go.jp/browse/jrd/70/1/_contents/-char/en
Review
Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control
Takehiko OGAWA, Takafumi MATSUMURA, Tatsuma YAO, Hiroshi KIMURA, Kiyoshi HASHIMOTO, Yu
ISHIKAWA-YAMAUCHI, Takuya SATO
Volume 70 Issue 1 Pages 1-9
DOI https://doi.org/10.1262/jrd.2023-093
Cover Story:
For the past century, achieving in vitro spermatogenesis has remained a difficult challenge for researchers. In 2011,Ogawa et al. successfully demonstrated in vitro spermatogenesis in mice using an organ culture method. However,extending this method to other species posed challenges for over a decade. In 2023, Ogawa’s team achieved in vitro
spermatogenesis in rats by incorporating several critical modifications to enhance their original technique. This
review presents a detailed analysis by Ogawa et al. comparing their method with natural in vivo conditions and othersynthetic alternatives (Ogawa et al. Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants,
tissue-form design, and space control, pp. 1–9). They systematically explore the merits, limitations, and inherent
constraints of the organ culture approach, delving into the specifics of medium composition, the principles of the gasliquid interphase method, use of microfluidic devices, and innovation of the PDMS-ceiling method. Highlighting thechallenges faced, including regulating oxygen concentration, managing tissue formation, and regulating culture
space-control. The insights and novel concepts shared in this review are particularly valuable for those involved inculture or related disciplines, providing innovative content, and encouraging further exploration in this field.
Original Article
PABPN1L is required for maternal mRNA degradation after meiosis resumption
Chihiro EMORI, Mayo KODANI, Ferheen ABBASI, Masashi MORI, Masahito IKAWA
Volume 70 Issue 1 Pages 10-17
DOI https://doi.org/10.1262/jrd.2023-077
Lipid droplet formation is spatiotemporally regulated in oocytes during follicular development in mice
Ryutaro AIZAWA, Megumi IBAYASHI, Junichiro MITSUI, Satoshi TSUKAMOTO
Volume 70 Issue 1 Pages 18-24
DOI https://doi.org/10.1262/jrd.2023-055
Development of a fluorometric Cuboni test for the semi-quantitative measurement of urinary estrogen levels andpregnancy detection in mares
Kaede ODA, Maya YOSHIDA, Abdul Razaq IRSHAD, Tomomi KANAZAWA, Toru TAKAHASHI
Volume 70 Issue 1 Pages 25-29
DOI https://doi.org/10.1262/jrd.2023-083
Equine chorionic gonadotropin treatment and timed artificial insemination for dairy cow production under heat stress
Daisuke FUNAKOSHI, Hidetoshi SHIOTANI, Makoto SEKI
Volume 70 Issue 1 Pages 30-34
DOI https://doi.org/10.1262/jrd.2023-069
Negative photoperiod induces an increase in the number of ovulations in dairy cattle
Fernando LÓPEZ-GATIUS
Volume 70 Issue 1 Pages 35-41
DOI https://doi.org/10.1262/jrd.2023-075
Serum-free spontaneously immortalized bovine oviduct epithelial cell conditioned medium promotes the early
development of bovine in vitro fertilized embryos
Norikazu MIYASHITA, Satoshi AKAGI, Tamas SOMFAI, Yuji HIRAO
Volume 70 Issue 1 Pages 42-48
DOI https://doi.org/10.1262/jrd.2023-031
Technology Report
Capturing temperature changes on the ocular surface along with estrus and ovulation using infrared thermography inJapanese Black cows
Riho OZAKI, Seiji INOUE, Yuki YOROZUI, Rei ICHIKAWA, Naoki YAMADA, Seiya HIGASHI, Shuichi
MATSUYAMA, Hiroko TSUKAMURA, Satoshi OHKURA, Yoshihisa UENOYAMA, Yasuhiro MORITA

Volume 70 Issue 1 Pages 49-54
DOI https://doi.org/10.1262/jrd.2022-116