Biological Buffers
Biological Buﬀers are widely used to maintain the pH within the spectrum of physilogical pH range.
We oﬀer a variety of buﬀers, including Tris, which is currently widely used, and HEPES, which has less
eﬀect on living organisms.

Buffer Grades
Cost & Quality

GradesTypical Specifications

Typical UsesHigh

BiopurSterility test, Endotoxin, Nuclease-freeFinest quality for life sciencefor biochemistry

Absorbance, Metal

General life science applications

for molecular biology

Nuclease-freeMolecular biology applications

SpecialGuaranteed reagent items, Absorbance

Cost-effective applications

Guaranteed reagent

JIS (Japanese Industrial Standards)

General Lab Use

Low

Biological Buffers pH range
Product list
Synonyms

Chemical Name

M.W.

Appearance

Bis-Tris 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol

209.24

Crystalline powder

PIPES

Piperazine-1,4-bis(2-ethanesulfonic acid)302.37

Crystalline powder

MOPS

3-Morpholinopropanesulfonic acid209.26

SolidHEPES

N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid238.30

Crystalline powder

Tris

2-Amino-2-hydroxymethyl-1,3-propanediol

121.14

Crystalline powder

AMPD

2-Amino-2-methyl-1,3-propanediol

105.14

Crystalline powder

AMP

2-Amino-2-methyl-1-propanol

89.14

Solid or Liquid

Synonyms

Chemical Name/Product Name

Bis-Tris

5.7

7.3

6.1

7.5

6.5

7.9

6.8

8.2

7.2

9.0
7.8

9.7
9.0

10.5

GradesSize

Cat.No.

2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol

for molecular biology

25 g04230-33

PIPES

Piperazine-1,4-bis(2-ethanesulfonic acid)for molecular biology

100 g32564-23

MOPS

3-Morpholinopropanesulfonic acidfor molecular biology

100 g25720-23

HEPES

N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acidfor molecular biology

100 g

18356-23

Special

500 g01946-08

Special

10 kg01946-84

for biochemistry

100 g40326-23

for biochemistry

500 g40326-08

for biochemistry

15 kg

40326-84

for molecular biology

100 g40326-24

Biopur500 g40326-09

Tris

2-Amino-2-hydroxymethyl-1,3-propanediol

AMPD

2-Amino-2-methyl-1,3-propanediol

Inquiry

InquiryーAMP

2-Amino-2-methyl-1-propanol

Inquiry

InquiryーTopic 2-Amino-2-hydroxymethyl-1,3-propanediol
In the biochemistry field, 2-amino-2-hydroxymethyl-1,3-propanediol (Tris) is widely used as a buffer
agent. The “Special” grade tested “appearance” and "absorbance (260 nm, 280 nm)," which indicatesthe optical absorption of nucleic acids and proteins, added to the “guaranteed reagent*” items.
This product is designed to provide the balance between quality and cost that is required in researchand development in the biochemical field, and to enhance cost performance.

Kanto Reagents＊guaranteed reagent : Guranteed JIS (Japanese Industrial Standards) items. JIS is a Japanese national standard that defines
standards and measurement methods for Japanese industrial products.

Special2-Amino-2-hydroxymethyl-1,3-propanediol● In addition to the “guranteed reagent’” items,"absorbance (260 nm, 280 nm)" and "appearance" items are guaranteed.
● High cost performance with emphasis on the balance between quality and cost.
● Bulk supply available ＊Please contact us for details.
Grades Comparision
Specification

SpecialCat. No.

01946-08 / 01946-84

Size

500 g / 10 kg

25 g / 500 g / 15 ㎏

100 g / 500 g / 15 ㎏

Puritymin. 99.0 ％min. 99.0 ％min. 99.0％

Appearance

to pass test

Solubility

to pass test

to pass test

to pass test

Melting point

169 - 173 ℃

169 - 173 ℃

169 - 173 ℃

Absorbance（260 nm）

max. 0.1

Absorbance（280 nm）

max. 0.08

Loss of drying

max. 0.5 %Guaranteed reagent

for biochemistry40326-30 / 40326-00 / 40326-80 40326-23 / 40326-08 / 40326-84

max. 0.05
max. 0.5 %Watermax. 0.2%

Residue on ignition (sulfate)max. 0.05 ％max. 0.05 %pH（0.1 mol/L, 25 ℃）

10.0 - 10.8

10.0 - 10.8

Chroride (Cl)max. 0.001 ％max. 0.001 ％max. 0.001 ％Sulfate (SO4)max. 0.001 ％max. 0.001 ％max. 0.001 ％Copper (Cu)max. 5 ppm

max. 5 ppm

max. 1 ppm

Lead (Pb)max. 5 ppm

max. 5 ppm

max. 1 ppm

Iron (Fe)max. 5 ppm

max. 5 ppm

max. 1 ppm

max. 0.005 %Magnesium (Mg)max. 1 ppm

Calcium (Ca)max. 3 ppm

Arsenic (As)max. 1 ppm

Manganese (Mn)max. 0.1 ppm

2024. 3