Cica Geneus Extraction Reagent Series
TM

Cica GeneusTM DNA Extraction Reagent
product number

Standard

08178-96

Cica GeneusTM DNA Extraction Reagent ST
product number

Strong08210-96

Cica GeneusTM DNA Extraction Reagent AN
product number

08199-96

For Animal

Cica GeneusTM RNA Extraction Reagent
product number

08211-96

For preparetion of RT-PCR template
Cica GeneusTM Extraction reagent series are reagent for eﬃcient extraction of genome DNA or total RNA from cell.
You can prepare PCR template in simple operation（Mix reagents andtarget samples, Incubate）

Cica GeneusTM DNA Extraction Reagent

Sample 10 μL
Reagent 100 μL

Preparetion of PCR templete only 10 minutes

a : 10 μL
b : 100 μL

Incubate
72℃―6 minutes
94℃―3 minutes

Collect supernatant■SampleStaphylococcus aureus ATCC® 6538TM■Target gene
16S rDNA (Ribosomal DNA)
■Culture condition
LB medium (30℃ 18 h)

Kanto ChemicalM
123~8

1 kbp DNA Ladder
Negative control
Positive control(Purified DNA)
Extracted DNA
10 serial dilution

Application

CicaGeneusTM DNA Extraction Reagent

Bacteria(from colony)

Yeast(from colony)

Conformity example
Gram positiveGram negativeM
1~34, 85~7100 bp DNA ladder
DNA extracts by this kit (n=3)
Negative control
DNA extracts by boiling (n=3)

M
1~34, 85~7

■SampleStaphylococcus aureus NBRC 102141(1~2 μL of colony)■Target gene
COA (Coagulase Ⅶ)
femA (Methicillin resistant factor)■Culture condition
SCD plate medium(37℃ overnight, aerobic)

100 bp DNA ladder
DNA extracts by this kit (n=3)
Negative control
DNA extracts by boiling (n=3)■SampleSaccharomyces cerevisiae ATCCTM 9763
(5~10 μL of colony)■Target gene
URA3 (decarboxylase)■Culture condition
Sabouraud glucose plate medium(37℃ overnight, aerobic)

CicaGeneusTM DNA Extraction Reagent STFungi

Other organisms

Please note that PCR amplification efficiency
varies according to PCR conditions such asenzymes and primers.

Application 2

Strong version increases the extraction efficiency
Yeast (from colony)
Colony 1 μL
Reagent 100 μL
Incubate
65℃―6 min
94℃―3 minDNA extracts(supernatant)
2 μL
PCR
PCR premix
18 μL Electrophoresis

Templete DNA
AptaTaq DNA Master (5x conc.)
Primer-Forward (10 pmol/μL)
Primer-Reverse (10 pmol/μL)
Sterile waterTotal
PCR condition: 94℃ 30 seconds
48℃ 90 seconds
72℃ 60 seconds
72℃ 7 minutes

2.0 μL4.0 μL1.0 μL1.0 μL
12.0 μL
20.0 μL
30 cycles

M 100 bp DNA ladder
1 DNA extracts by this kit
2 DNA extracts by boiling

M 100 bp DNA ladder
1 DNA extracts by this kit
2 DNA extracts by CicaGeneus®
DNA Extraction Reagent■SampleSaccharomyces cerevisiae
ATCCTM 9763
(1 μL of colony)■Target gene
URA3 (939 bp)■Culture conditionSabouraud glucose plate medium(37℃ overnight, aerobic)■SampleStaphylococcus aureus
NBRC 102141(1~2 μL of colony)■Target gene
16S rRNA (324 bp)■Culture condition
SCD plate medium(37℃ overnight, aerobic)

Application 3

CicaGeneusTM DNA Extraction Reagent AN

Optimized version for animal tissue samples
Mouse tail
Mouse tail3 mm (15 mg)
Reagent 100 μL
Incubate
65℃―6 min
94℃―3 minCentrifuge 10,000×g, 5 minutes

DNA extracts(supernatant)
3-fold diluted
2 μL

PCR

PCR premix 18 μL Electrophoresis

Templete DNAAptaTaq DNA Master (5x conc.)
Primer-Forward (10 pmol/μL)
Primer-Reverse (10 pmol/μL)
Sterile waterTotal

2.0 μL4.0 μL1.0 μL1.0 μL
12.0 μL
20.0 μL

PCR condition: 94℃ 30 seconds
60℃ 90 seconds
72℃ 60 seconds

30 cycles

72℃ 7 minutes

CicaGeneusTM RNA Extraction Reagent

M 100 bp DNA ladder
1 DNA extracts by this kit
2 DNA extracts by boiling

M 100 bp DNA ladder
1 DNA extracts by this kit
2 DNA extracts by boiling■SampleMouse tail (3 mm)■Target gene
β-globin (494 bp)■SampleHuman whole blood added EDTA (1 μL)■Target gene
ALDH2 (176 bp)

Application 4

You can prepare total RNA for RT-PCR without using organic solvent
Staphylococcus aureus (from colony)

Reagent 100 μL
a : 10 μL
b : 100 μL■SampleStaphylococcus aureus■Target gene
16S rRNA (324 bp)■Culture condition
SCD plate medium(37℃ overnight, aerobic)

Sample 10 μL

Incubate
65℃―6 min
94℃―3 min

Confomity example

10,000×g 5 min

Collect supernatant
5 μL

Incubate●Gram positive

37℃―15 min
75℃―5 min●Gram negative●FungiDNA degradation solution 5 μL
c : totald : 630 μL

RNase free water 45 μL

Templete RNA
RTreaction 5x PrimeScript Buffer

PrimeScript RT Enzyme Mix
Primer-Reverse (2 pmoL/μL)
Sterile waterTotal

5.0 μL2.0 μL0.5 μL0.5 μL2.0 μL10.0 μL

RNA extracts
RT condition:
42℃ 15 min
85℃ 5 sec

M 100 bp DNA ladder
1 RNA extracts by this kit
2 Negative control●Animal cells

RT reaction solution
2.0 μL
AptaTaq DNA Master (5×conc.) 4.0 μL
Primer-Forward (10 pmol/μL) 1.0 μL
Primer-Reverse (10 pmol/μL) 1.0 μL
Sterile water
12.0 μL
20.0 μL
Total

PCR condition:
94℃ 30 sec
54℃ 90 sec
72℃ 60 sec
72℃ 7 min30cyclesPCR related products
AptaTaq DNA Master（5 X Conc.）●Hot start PCR enzyme●Optimal for PCR and Real-Time PCR etc.
●Ready-to-Use
AptaTaq DNA Polymerase（AptaTaq）is hot start type ampliﬁcation enzyme that combineshigh speed and intensity. AptaTaq is bound by aptamer such as oligonucleotides withreversible manner (non-covalent). This aptamer has temperature dependent tertiary
structure, it works as switch of enzyme activity. This polymerase activity is inactive intemperature below 55 ℃. On the other hand, AptaTaq could be used for hot start type polymerase in temperature over 60 ℃ using aptamer type polymerase, you can save PCR timecompared with chemical modiﬁed polymerase.
AptaTaq DNA Master（×5）is Ready-to-use PCR master mix includes this AptaTaq with substrate and Mg ion. Adding primers and templete DNA (ex. genomic DNA, cDNA, plasmid) into
this reagent, you can do PCR with high eﬃciency.
Product number01016-96 500 µL (125 rxns)※for 20 µL

Product information
Product No.

Product Name

Package size

Sｔｏｒｅd atTM08178-96

CicaGeneus

DNA Extraction Reagent

120 rxns

2 ℃〜8 ℃

08210-96

CicaGeneus

TM

DNA Extraction Reagent ST

120 rxns

2 ℃〜8 ℃

08199-96

CicaGeneus

TM

DNA Extraction Reagent AN

120 rxns

2 ℃〜8 ℃

08211-96

CicaGeneus

TM

RNA Extraction Reagent

120 rxns

2 ℃〜8 ℃

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KANTO CHEMICAL CO., INC.
REAGENT DIVISION

East Muromachi Mitsui BLDG, 2-1, Nihonbashi Muromachi 2-chome,Chuo-ku, Tokyo, 103-0022, JAPAN
Telephone+81-3-6214-1092
Telefax+81-3-3241-1053
http://www.kanto.co.jp
E-mail; kanto-61@gms.kanto.co.jp
2015. August.