Cica Geneus® Purification Kit Series

溶解

Cica Geneusa® Purification Kit help withsuccessful experiments！

結合洗浄

シカジーニアスシリーズに待望の新製品！
Product

Information

Product No.

Product Name

08197-9608204-9608204-97

Stored at

Size

Cica GeneusⓇ DNA Prep Kit (for Blood & Tissue)RT

100 preps

Cica GeneusⓇ Total DNA Prep Kit (for Tissue)RT

100 preps
250 preps

08202-96

Cica Geneus Ⓡ DNA Prep Kit (for Plant)RT

100 preps

08203-96

Cica Geneus Ⓡ DNA Prep Kit (for Stool)RT

50 preps

Cica Geneus Ⓡ Plasmid Prep Kit

RT08206-96

Cica Geneus Ⓡ PCR Prep Kit

RT

50 preps

08208-96

Cica Geneus Ⓡ Gel Prep Kit

RT

50 preps

Cica Geneus Ⓡ PCR & Gel Prep Kit

RT08207-96
08207-97

08111-96
08111-97
08215-96

Cica Geneus Ⓡ DNA/RNA Prep Kit (for Virus)

08057-9608058-96

50 preps
200 preps

50 preps
200 preps

２〜８℃

50 preps

Cica Geneus Ⓡ RNA Prep Kit (for Tissue)RT

50 preps

Cica Geneus Ⓡ RNA Prep Kit (for Plant)RT

50 prepsCica GeneusⓇ Purification Kit Appearance

Each buffers have a inner lid
Resuce the contamination risk !!

We provide the most suitable columns
depending on various samples !!

Type D

Type F

Type G

Type Q

Type V

Type W

Columns for DNA are packed in the box.
Columns for RNA are packed in the blister to prevent contamination !!

Binding
capacity

Minimum
elutionvolumeYield/Recovery

Operation
time

Cica GeneusⓇ DNA Prep Kit (for Blood & Tissue)

60 μg

30 μL

20~60 μg25~220 min

Cica GeneusⓇ Total DNA Prep Kit (for Tissue)

50 μg

30 μL

5~50 μg25~220 min

Cica GeneusⓇ DNA Prep Kit (for Plant)

40 μg

30 μL

4~40 μg~40 minCica GeneusⓇ DNA Prep Kit (for Stool)

100 μg30 μL

5~100 μg

~25 minCica GeneusⓇ Plasmid Prep Kit

30 μg

40 μL

85~95%~23 minCica GeneusⓇ PCR & Gel Prep Kit or PCR Prep Kit

10 μg

30 μL

90~95%（100 bp~10 kb）~5 minCica GeneusⓇ PCR & Gel Prep Kit or Gel Prep Kit

10 μg

30 μL

70~85%(80 bp~10 kb)~15 minCica GeneusⓇ DNA/RNA Prep Kit (for Virus)

100 μg30 μL―~20 minCica GeneusⓇ RNA Prep Kit (for Tissue)

100 μg40 μL~20 μg（cell）~50 μg（Tisuue）~15 minCica GeneusⓇ RNA Prep Kit (for Plant)

100 μg30 μL~50 μg~25 min

Product Name

1

Cica GeneusⓇ Purification Kit Selection Guide
Meaning of the icons
Animal Tissue/Mouse tail

Mammalllian whole blood

Callus

PCR products

Cultured cells

Insect / Worm

Fungi

Agarose gelGram(+) bacteria

Buccal swab/Buffy coat/
Hair/Body fluid/Saliva/
Sperm

Stool

Cultured cells/Plasma/
Serum/swab/Urine

Gram(-) bacteria

Plant cells/Plant tissue

Escherichia coli

Selection Guide
SampleType of the nucleic acid
DNA

GenomeWhole blood
Insect/Worm
Mouse tailAnimal tissue
Cultured cells
Gram(-) bacteria

Buffy coat
Animal tissue
Hair
Cultured cellGram(+) bacteria Body fluid
Gram(-) bacteria Saliva
Spem
Whole blood
Mouse tailBuccal swab
Streptococcus pneumoniae
Plant cells
Plant tissue

CallusFungi

Recommended product
Cica GeneusⓇ DNA Prep Kit (for Blood & Tissue)

Cica GeneusⓇ Total DNA Prep Kit (for Tissue)

Cica GeneusⓇ DNA Prep Kit (for Plant)

Stool

Cica GeneusⓇ DNA Prep Kit (for Stool)

Plasmid

Escherichia coli

Cica GeneusⓇ Plasmid Prep Kit

Fragment DNA

PCR products

Cica GeneusⓇ PCR & Gel Prep Kit or PCR Prep Kit

Agarose gel

Cica GeneusⓇ PCR & Gel Prep Kit or Gel Prep Kit

DNA/RNA

Viral nucleic acid

Cultured cells
PlasmaSerum

RNA

total RNA

Animal tissue
Bacteria
Cultured cells
Whole blood cell

Cica GeneusⓇ RNA Prep Kit (for Tissue)

Plant cells
Plant tissue

Cica GeneusⓇ RNA Prep Kit (for Plant)

Swab
Urine

Cica GeneusⓇ DNA/RNA Prep Kit (for Virus)

2

Ⓡ

Cica Geneus DNA Prep Kit (for Blood & Tissue)
Product No.

Size

08197-96

100 preps

Cica GeneusⓇ DNA Prep Kit (for Blood & Tissue) provides asimple and rapid method for the isolation of totalDNA fromanimal tissues, whole blood, and cultured cells. The pure DNA
can be acquired and this can be directly used in various

Protocol for Animal Tissue
Lyse
1） Sample 25 mg
2）②Buffer TL：200 μL
Vortex for 1 min3）Proteinase K solution：20 μL
Vortex for 1 min

applications such as PCR, Southern blotting and otherenzymatic reactions.

Specifications
Liquid：300 μL
Solid： 25 mg

Sample size
Maximum loading volume

750 μL

Binding capacity

60 μg

Elution volume

30~400 μL

Kit Contents
Incubate at 56℃ until the sample is completely lysed4）③Buffer TB：400 μL
VortexCentrifuge for 1 min at 6,000×g

Bind

size

GeneAll SV Column Type G（with collection tube）

50 ×2

Collection tube

100 ×2①Buffer RL

100 mL×1②Buffer TL

30 mL×1③Buffer TB

50 mL×1④Buffer BW

80 mL×1⑤Buffer TW

100 mL×1⑥Buffer AE

30 mL×1

Transfer to a new collection tube

⑦Proteinase K

48 mg×1

5）④Buffer BW：600 μL⑧Storage Buffer

4 mL×1

Transfer the mixture to the Gene All SV Column type G

Centrifuge for 1 min at 6,000×g

Wash

Contents name

Centrifuge for 30 sec at 6,000×g
Transfer to a new collection tube
6）⑤Buffer TW：700 μL
Centrifuge for 30 sec at 6,000×g
Discard the pass-through and reinsert

DNA Puriﬁcation from various samples
DNA prepared from several kind of animal tissue using Cica
GeneusⓇ DNA Prep Kit (for Blood & Tissue). Elution was
performed with100 μL of ⑥Buffer AE.
8 μL of luates was resolved on 0.8 % agarose gel.

1

2

3

4

5

6

7

8

9

Centrifuge for 30 sec at full speed

Elute

Place the column in a fresh 1.5 mL tube
7）⑥Buffer AE：200 μL（or sterilized water）
Incubate for 2 minCentrifuge for 1 min at 13,000×g

DNA solution3

Lane 1：Liver (rat)Lane 5：Lung (rat)Lane 2：Kidney (rat)Lane 6：Heart (rat)Lane 3：Brain (rat)Lane 7：NIH/3T3 cell

Lane 4：Spleen (rat)Lane 8：Blood (bovine)
Lane 9：Liver (bovine)

Ⓡ

Cica Geneus Total DNA Prep Kit (for Tissue)
Cica Geneus Ⓡ Total DNA Prep Kit (for Tissue) provides an

Product No.

Size

08204-96

100 preps

08204-97

250 prepseasy and rapid method for the isolation of total DNA such as
genomic, mitochondrial, bacterial, parasite or viral DNA fromvarious samples including tissues, whole blood, cultured cells,
and body fluids. The purified DNA is suitable for PCR,Southern blotting other enzymatic reactions, and etc.

Related reagent
Product No.

Product Name

Size

14032-08

Ethanol (99.5)

500 mLSpecifications
Maximum loading volume

700 μL

Binding capacity

50 μg

Protocol for Cultured Cell
Lyse1）Proteinase K solution：20 μL
2）Sample：200 μL
3）②Buffer BL：200 μL
Vortex
Incubate at 56℃ until the sample is completely lysed4）Ethanol：200 μL（not provided）
VortexSpin down

BindTransfer the mixture to the Gene All SV Column type G

Liquid：200 μL
Solid： 20 mg

Sample size

Elution volume

30~400 μL

Kit Contents
Contents name

100 preps

GeneAll SV Column Type G

Size

250 preps

50×2

50×5

Collection tube

100×2

250×2

①Buffer CL

25 mL×1

60 mL×1②Buffer BL

25 mL×1

60 mL×1③Buffer BW

80 mL×1

220 mL×1④Buffer TW

100 mL×1

250 mL×1⑤Buffer AE

30 mL×1

60 mL×1⑥Proteinase K

48 mg×1

120 mg×1⑦Storage Buffer

4 mL×1

7 mL×1（with collection tube）

Centrifuge for 1 min at 6,000×g

Wash

Transfer to a new collection tube

DNA purification from Whole blood5）③Buffer BW：600 μL

DNA purified from whole blood using Cica GeneusⓇ Total DNA
Prep Kit (for Tissue) was resolved on 0.8 % agarose gel.

Centrifuge for 30 sec at 6,000×g

1

2

3

4

5

6

7

8

Transfer to a new collection tube
6）④Buffer TW：700 μL
Centrifuge for 1 min at 6,000×g
Discard the pass-through and reinsert

Centrifuge for 30 sec at full speed

Elute

Place the column in a fresh 1.5 mL tube
7）⑤Buffer AE：200 μL（or sterilized water）
Incubate for 1 min
Centrifuge for 1 min at 13,000×g

DNA solution

Lane 1,2：Upper

human blood A Lower

rat blood A

Lane 3,4：Upper

human blood B Lower

rat blood B

Lane 5,6：Upper

human blood C

Lower

rat blood C

Lane 7,8：Upper

human blood D

Lower

rat blood D

4Ⓡ

Cica Geneus DNA Prep Kit (for Plant)
Product No.

Size

08202-96

100 preps

Protocol for Plant tissue
Lyse

Grind fresh (~100 mg) or frozen plant tissue(~25 mg)
to a fine powder quickly and completely,using amortar and pestle pre-cooled with liquid nitrogen

Cica GeneusⓇ DNA Prep Kit (for Plant) provides an easy andrapid method for the isolation of total DNA from various Plant.
With EzSep filter and Column typeG, the procedure can bedone in just 40 minites (mini). The purified DNA is suitable for
PCR, Southern blotting other enzymatic reactions, and etc.

Specifications
Wet：100 mg
Dry： 25 mg

Sample size1）Sample2）①Buffer PL：400 μL
3）⑥RNase A：4 μL
Vortex for 1 min
Incubate at 65℃ for 10 ~ 15 min＊Mix 2 ~ 3 times by inverting

Maximum loading volume

750 μL

Binding capacity

40 μg

Elution volume

30~400 μL

Kit Contents
Contents name4）②Buffer PD：140 μL
Vortex

Incubate for 5 min on ice
Transfer the mixture to the EzSep filter

Centrifuge for 2 min at 14,000×g

Size

GeneAll SV Column Type G（with collection tube）

50×2

EzSep filter（with collection tube）

50×2①Buffer PL

100 mL×1②Buffer PD

30 mL×1③Buffer BD

110 mL×1④Buffer CW

150 mL×1⑤Buffer AE

60 mL×1⑥RNase A (100 mg/ml)

480 μL×1

5）Transfer to a new collection tube
6）③Buffer BD： 1.5 vol of 5)

DNA Purification from various samples

Bind

DNA was purified from each 100 mg of various samples Cica
Transfer the mixture to
the Gene All SV Column type G

GeneusⓇ DNA Prep Kit (for Plant). Elution was performed with
100 μL of ⑤Buffer AE. 5 μL of luates was resolved on 0.8 %agarose gel.

Centrifuge for 30 sec at 10,000×g

Wash

M

1

2

3

4

5

6

Discard the pass-through and reinsert
7）④Buffer CW：700 μL

Centrifuge for 30 sec at 10,000×g
ろ液を捨てカラムを再セット
8）④Buffer CW：700 μL

Centrifuge for 2 min at 10,000×g

Elute

Place the column in a fresh 1.5 mL tube
9）⑤Buffer AE：200 μL

Incubate for 5 minCentrifuge for 1 min at 10,000×g

DNA solution
5

M：LadderLane 1：Pine

Lane 5：Cayenne

Lane 2：Tabacco

Lane 6：Arabidopsis

Lane 3：Barely

Lane 7：Potato

Lane 4：Cycad

7

Ⓡ

Cica Geneus DNA Prep Kit (for Stool)
Product No.

Size

08203-96

50 preps

rapid method for the isolation of DNA from stool samples.The
purified DNA is suitable for PCR, Southern blotting otherenzymatic reactions, and etc.

Protocol for Stool
Lyse

Cica GeneusⓇ DNA Prep Kit (for Stool) provides an easy andSpecifications

2.0 mL tube
1）Sample：〜200 mg
2）①Buffer PBS：1 mL
Vortex for 1 min
Incubate for 30 sec
Transfer the supernatant to a new 2.0 mL tube
Centrifuge for 2 min at full speed3）Discard the supernatant

4）②Buffer FL：1.3 mL

Resuspend the pellet by pipetting
Incubate for 5 minCentrifuge for 5 min at 10,000×g

Transfer the mixture to Gene All EzPass filter

Sample size

200 mg(Stool)Maximum loading volume

700 μL

Binding capacity

100 μgElution volume

30~200 μL

Kit Contents
Contents name

Size

GeneAll SV Column Type G（with collection tube）

50 ×1

GeneAll EzPass filter（with collection tube）

50 ×1

1.5 ml tube

50 ×2
50 ×2

2.0 ml tube
Centrifuge for 1 min at 10,000×g

Transfer the Gene All EzPass filter to the new 1.5 mL tube
5）③Buffer EB：100 μL
Incubate for 1 min①Buffer PBS

60 mL×1②Buffer FL

70 mL×1③Buffer EB

15 mL×1④Buffer PB

30 mL×1⑤Buffer NW

30 mL×1

Centrifuge for 1 min at 10,000×g

6）④Buffer PB：500 μL
Resuspend the pellet by pipetting

DNA purification from Chicken stool
DNA was purified from Chicken stool, using Cica GeneusⓇ DNA
Prep Kit (for Stool). Elution was performed with 50 μL of

Bind
Transfer the mixture to Gene All SV Column type G③Buffer EB. 3 μL of luates was resolved on 1.0 % agarose gel.

M

1

2

3

4

Centrifuge for 1 min at 10,000×g

Wash

Discard the pass-through and reinsert

7）⑤Buffer NW：500 μL
Centrifuge for 1 min at 10,000×g
Discard the pass-through and reinsert

Centrifuge for 1 min at 10,000×g

Elute

Place the column in a fresh 1.5 mL tube

8）③Buffer EB：50 μL
Incubate for 1 min

Centrifuge for 1 min at 10,000×g

DNA solution

Lane 1-4：Chicken stool
M：1kb Ladder

6

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Cica Geneus Plasmid Prep Kit
Product No.

Size

08207-96

50 preps

08207-97

200 preps

Cica GeneusⓇ

Plasmid Prep Kit provides a convenient andrapid method for the isolation of plasmid DNA from bacterial
cells. This kit eliminates the need of organic solvent extraction
and alcohol precipitation, allowing rapid and convenientprification from many samples simultaneously. The purified
DNA is suitable for PCR, cloning, automated sequencing,

Protocolsynthesis of labeled hybridization probes and other enzymatic
reactions, and etc.

Lyse

Specifications1）Sample（bacterial cultures）：5 mL

Centrifuge for 1 min at full speed3）②Buffer S2：250 μL

800 μL

Binding capacity

30 μg
40~200 μL

Mix 2 ~ 3 times by inverting

Contents name4）③Buffer S3：350 μL
Mix 2 ~ 3 times by inverting

GeneAll SV Column Type Q

Centrifuge for 30 sec at 10,000×g
Discard the pass-through and reinsert
5）④Buffer AW：500 μL

100 prepsSize

200 preps

50×1

50×4

①Buffer S1

20 mL×1

60 mL×1②Buffer S2

20 mL×1

60 mL×1③Buffer S3

25 mL×1

90 mL×1④Buffer AW

30 mL×1

110 mL×1⑤Buffer PW

60 mL×1

250 mL×1⑥Buffer EB

15 mL×1

30 mL×1⑦RNase A solution

100 μL×1

300 μL×1（with collection tube）

Transfer the supernatant toGene All SV Column type Q

Wash

Maximum loading volume

Kit Contents

Centrifuge for 10 min at 10,000×g

Bind~10 mL

Elution volume2）①Buffer S1：250 μL
Resuspend the pellet by pipetting

Sample size

＊Add all of ⑦RNase A Solution to ①Buffer S1 before first use.

Centrifuge for 30 sec at 10,000×g

Enzyme Digestion

Discard the pass-through and reinsert

Prep Kit were subjected to digestion by restriction enzyme.

6）⑤Buffer PW：700 μL

Several kinds of plasmid DNA purified with Cica GeneusⓇ Plasmid

M

1

2

3

4

5

6

Centrifuge for 30 sec at 10,000×g

Discard the pass-through and reinsert

Centrifuge for 30 sec at 10,000×g

Elute

Place the column in a fresh 1.5 mL tube
7）⑥Buffer EB：50 μL
Incubate for 1 min
Centrifuge for 1 min at 10,000×g

DNA溶液
7

M：Lambda-BatPl
Lane 1：pUC18
Lane 2：pUC18, digested with Xbal
Lane 3：pQE30
Lane 4：pQE30, digested with Smal
Lane 5：pBluescript Ⅱ SK (+)
Lane 6：pBluescript Ⅱ SK (+), digested with Xbal

M

Ⓡ

Cica Geneus PCR Prep Kit
Product No.

Size

08206-96

50 preps

Cica GeneusⓇ PCR Prep Kit is for purification of PCR product.
It can recover wide range size of DNA (100 - 10 kb) and remove
small DNA ( < 100 bp) such as primer and primer-dimer.
The purified DNA is suitable for sequencing, cloning and etc.

ProtocolSpecifications

Lyse1）①Buffer PB：5-times to sample

Sample volume

100 μL

Maximum loading volume

700 μL

Binding capacity

10 μg

Elution volume

Bind

30~200 μL

Transfer the mixture to the Gene All SV Column type D

Kit Contents
Contents name

Size

GeneAll SV Column Type D

50 ×1（with collection tube）

Centrifuge for 30 sec at 10,000×g

Wash

Transfer to a new collection tube

2）②Buffer NW：700 μL①Buffer PB

30 mL×1②Buffer NW

60 mL×1③Buffer EB

15 mL×1

Centrifuge for 30 sec at 10,000×g

PCR products : Bofore and After purification

Transfer to a new collection tube

Valous size of PCR products were resolved on 1.0 % agarose gel.

Elute

M

1

2

3

4

5

6

Place the column in a fresh 1.5 mL tube
3）③Buffer EB：50 μL

Incubate for 1 min

Centrifuge for 1 min sec at 10,000×g

6

DNA solution

Sample volume：3 μg

M：phi-x174-HaeⅢ

DNA size ： 494 bp

Lane 1：PCR product（ 312 bp ）, Before purification

Recovery rates（%）

100

Lane 2：PCR product（ 312 bp ）, After purification

3

Lane 3：PCR product（ 850 bp ）, Before purification

50

0

1.5

20

30

50

80

Volume of Buffer EB （μL）

100

Yield（μg）

DNA concentration（μg/mL）

Recovery rates（％）

Lane 4：PCR product（ 850 bp ）, After purification
Lane 5：PCR product（ 1.6 kp ）, Before purification
Lane 6：PCR product（ 1.6 kp ）, After purification

0

8

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Cica Geneus Gel Prep Kit
Cica GeneusⓇ Gel Prep Kit can provide easy and rapid method for
Product No.

Size

08208-96

50 preps

purification from standard agarose gel useing TAE, TBE Buffer andlow-meltiong agalose gel.
It can purify wide range size of DNA (80 - 10 kb) from various

Related reagent

grade agarose.

Product No.

Product Name

Size

32435-09

2-Propanol

500 mL

ProtocolCut the target DNA from
agarose gel to use as sample

Lyse

Specifications
Sample volume (Agarose gel)

400 mgMaximum loading volume

700 μL

Binding capacity

10 μg

Elution volume

30~200 μL

Kit Contents
Contents name1）Sample2）①Buffer GB：Ｘ μL( 3 μL / 1 mg sample)

GeneAll SV Column Type D

50×1（with collection tube）

Incubate at 65 ℃ for 5 - 10 min※ Vortex per 2 -3 min
Yellow solution is conformed
3）2‐Propanol ：Y μL( 1 μL / 1 mg sample )Size①Buffer GB

60 mL×1②Buffer NW

60 mL×1③Buffer EB

15 mL×1

DNA purification from agarose gel
Various size of DNA were resolved on agarose gel (TAE).
Then, DNA were purified from agarose gel using Cica

Bind

GeneusⓇ Gel Prep Kit.
Transfer the mixture to the Gene All SV Column type

1

2

3

4

5

6

Centrifuge for 1 min at 10,000×g

Wash

Transfer to a new collection tube
4）①Buffer GB：500 μL

Centrifuge for 30 sec at 10,000×g
Transfer to a new collection tube
5）②Buffer NW：700 μL

Centrifuge for 30 sec at 10,000×g

EluteTransfer to a new collection tube

Lane 3：DNA fragment（ 1.3 kb ）, Before gel extraction / purification

Centrifuge for 1 min at 10,000×g

Lane 4：DNA fragment（ 2.3 kb ）, Before gel extraction / purification

Place the column in a fresh 1.5 mL tube
6）③Buffer EB：50 μL
Incubate for 1 min

Centrifuge for 1 min at 10,000×g

DNA solution
9

Lane 1：DNA fragment（ 466 bp ）, Before gel extraction / purification
Lane 2：DNA fragment（ 782 bp ）, Before gel extraction / purification

Lane 5：DNA fragment（ 5.0 kb ）, Before gel extraction / purification
Lane 6：DNA fragment（ 466 bp, 782 bp, 1.3 kb, 2.3 kb, 5.0 kb )*＊Pooled sample from lane 1-5 after extraction / purification

Ⓡ

Cica Geneus PCR & Gel Prep Kit
Product No.

Size

08111-96

50 preps

08111-97

200 preps

価格

Kit and Gel Prep Kit. You can purify PCR product by using
Buffer PB and agarose gel sample by using Buffer GB.

Protocol for PCR produstSpecifications≪ Sample : PCR product ≫Lyse1）①Buffer PB：5-times to sample

BindThis product is a combination kit of Cica GeneusⓇ PCR Prep

Transfer the mixture to the Gene All SV Column type D

Sample volume

100 μL

Maximum loading volume

700 μL

Binding capacity

10 μg
30~200 μL

Elution volume

Wash≪ Sample : In agarose gel ≫Centrifuge for 30 sec at 10,000×g
Transfer to a new collection tube
2）②Buffer NW：700 μL

Centrifuge for 30 sec at 10,000×g

400 mgMaximum loading volume

700 μL

Binding capacity

10 μg

Elution volume

Transfer to a new collection tube

Elute

Sample volume (Agarose gel)30~200 μL

Kit Contents

Place the column in a fresh 1.5 mL tube
3）③Buffer EB：50 μL

Contents nameIncubate for 1 min

50 preps

GeneAll SV Column Type D
Centrifuge for 30 sec at 10,000×g

DNA solution

Cut the target DNA from
agarose gel to use as sample

Lyse1）Sample

200 preps

50 ×1

50 ×4

①Buffer PB

30 mL×1

120 mL×1②Buffer GB

60 mL×1

120 mL×2③Buffer NW

60 mL×1

250 mL×1④Buffer EB

15 mL×1

30 mL×1（with collection tube）

Protocol for Agarose gel

Size2）①Buffer GB：Ｘ μL( 3 μL / 1 mg sample)

Incubate at 65 ℃ for 5 - 10 min※ Vortex per 2 -3 min
Yellow solution is conformed
3）2‐Propanol ：Y μL( 1 μL / 1 mg sample )

Bind

Wash

Transfer the mixture to the Gene All SV Column type

Centrifuge for 1 min at 10,000×g
Transfer to a new collection tube
4）①Buffer GB：500 μL

Centrifuge for 30 min at 10,000×g
Transfer to a new collection tube
5）②Buffer NW：700 μL

Recovery rate from each samples（％）
Sample volume：3 μg
Elution Buffer ： Buffer EB (50 μL)
DNA Size (bp)

PCR product (％)

Agarose gel (％)

60

0

39

120

78

71

200

83

76

800

94

84

1,800

91

82

4,300

85

78

8,700

76

73

Centrifuge for 30 min at 10,000×g
Transfer to a new collection tube
Centrifuge for 1 min at 10,000×g
Elute

Place the column in a fresh 1.5 mL tube
6）③Buffer EB：50 μL
Incubate for 1 min

Centrifuge for 1 min at 10,000×g

DNA solution

10

Cica Geneus DNA/RNA Prep Kit（for Virus）Ⓡ

Product No.

Size

08215-96

50 preps

Cica GeneusⓇ Total DNA/RNA Prep Kit (for Viral) provides aneasy and rapid method for the isolation of total DNA such ascell-free fluid, cell-culture supernatant, plasma, serum, swab,
urine and virus-infected samples.
The purified DNA/RNA is suitable for PCR, RT-PCR and etc.

ProtocolSpecifications
Lyse1）Sample： 300 μL
2）①Buffer VL：500 μL
3）Carrier RNA solution：5 μL**Prepare before it ﬁrst use

Incubate for 10 min
Spin-down

Sample volume

300 μL

Maximum loading volume

750 μL

Binding capacity

100 μgElution volume

30~50 μL

Kit Contents

Bind

Wash

Size

Contents name4）②Buffer RB1：700 μL

GeneAll SV Column Type V（with collection tube）

50 ×1

Collection tube

50 ×1

Vortex for 1 min①Buffer VL

30 mL×1

Transfer the mixture to②Buffer RB1

40 mL×1

the GeneAll SV Column Type V③Buffer RBW

30 mL×1④Buffer RNW

30 mL×1⑤Nuclease-free water

15 mL×1⑥Carrier RNA

270 μg×1

Centrifuge for 30 sec at 10,000×g
Transfer to a new collection tube

5）③Buffer RBW：500 μL

Amplification of HSV-1 (Herpes simplex virus) DNA
Centrifuge for 30 sec at 10,000×g

Fig.1

samples using Cica GeneusⓇ DNA/RNA Prep Kit (for Virus).

Transfer to a new collection tube

6）④Buffer RNW：500 μL

Purified DNA was amplified by PCR and comfirmed.

Fig.2

DNA was purified gradually diluted HSV-1 sample using

Centrifuge for 30 sec at 10,000×g

Cica GeneusⓇ DNA/RNA Prep Kit (for Virus).

Transfer to a new collection tube

Purified DNA was amplified by PCR and comfirmed.

Centrifuge for 1 min at 10,000×g

Elute

DNA was purified from cells infected by HSV-1 and HSV-1

Place the column in a fresh 1.5 mL tube

Fig.1
1 2

3

4

Fig.2
1 2

3

4

5

6

7

7）⑤Nuclease-free water：30~50 μL
Incubate for 1 min

Centrifuge for 1 min at 10,000×g

DNA/RNA solution
Lane 1：HSV-1 infected cell

Lane 1：HSV-1 sample ( 6×104 pfu )Lane 2,3：HSV-1 sample

Lane 2：HSV-1 sample( 6×103 pfu )Lane 4：Negative control

Lane 3：HSV-1 sample( 6×102 pfu )Lane 4：HSV-1 sample( 6×10 pfu )Lane 5：HSV-1 sample( 6 pfu )Lane 6：Negative control ( PCR )11

Lane 7：Negative control

Ⓡ
シカジーニアス®
Cica Geneus RNA
RNAプレップキッ
Prep Kit (for Tissue)
ト（組織用）
Product
No.
製品番号

容量
Size

08057-96

50
50preps
回分

Cica Geneus Ⓡ RNA Prep Kit (for Tissue) provides an easy
and rapid method for the isolation of total RNA such as
cultured cells, whole blood cells, animal tissues.
The purified RNA is suitable for cDNA synthesis, RT-PCR
and etc.

Related reagent
製品番号No. 製品名
Product
Product Name

Size
容量

2‐Mercaptoethanol
25099-30 2‐メルカプトエタノール

25 g

操作方法（組織サンプルの場合）
Protocolfor Tissue

25
25mg
mg
Tissues：
組織の場合：
6
6
cells
個
細胞の場合
Cells： 5×10
：5×10

Sample size
最大サンプル量
Maximum loading volume
カラムへの最大ローディング量750 μlμL
750

Binding RNA 量
capacity
最大結合100 μgμg
100

Elution volume
溶出量μL
40~100 μl

Kit Contents

溶解
Lyse1）Sample：25 mg
1）サンプル：25
mg2）①Buffer LYS：400 μLμl( ホモジナイズ )Grind,
homogenize
破砕・懸濁Incubate
静置 室温，10分間
for 10 min

結合
Bind

Specifications

Gene All theTransfer
SV mixture
Column to
type
theFに移す
Gene All SV Column type F

Contents name
個別名称

容量
Size

GeneAll SV Column Type F（with collection tube）（コレクションチューブ付）

50 50
個 ×1

1.5 mLml tube
tube

50 50
個 ×1

①Buffer LYS

30mL×1ml×1
30②Buffer GW1

35mL×1
ml×1
35③Buffer RNW

50mL×1
ml×1
50④RNase-free water

15mL×1ml×1
15

遠心 10,000×g，
Centrifuge
for 30 sec
室温，
at 10,000×g
30秒間

Wash
洗浄

ろ液を捨てカラムを再セット
Transfer
to a new collection tube
3）②Buffer GW1：500 μLμl
遠心 10,000×g，
Centrifuge
for 30 sec
室温，
at 10,000×g
30秒間
ろ液を捨てカラムを再セット
Transfer
to a new collection tube

RNA purification from HeLa cells
RNA purified from HeLa cells using Cica GeneusⓇ RNA
Prep Kit (for Tissue) and other company’ s products
were resolved on 1.0 % agarose gel.
M

1

2

3

4

4）③Buffer RNW：700 μLμl
遠心 10,000×g，
Centrifuge
for 30 sec
室温，
at 10,000×g
30秒間
ろ液を捨てカラムを再セット
Transfer
to a new collection tube

28S rRNA

遠心 10,000×g，
Centrifuge
for 1 min室温，
at 10,000×g
1分間

Elute
溶出

1.5 mlチューブにカラムをセット
Place
the column in a fresh 1.5 mL tube

18S rRNA

5）④RNase-free water：50 μLμl
静置 室温，1分間
Incubatefor 1 min

遠心 10,000×g，室温，1分間
Centrifuge
for 1 min at 10,000×g

RNA溶液
RNA
solution

M：LadderLane 1,2：Cica GeneusⓇ RNA Prep Kit (for Tissue)
Lane 3,4：Competitive products

12

Ⓡ

Cica Geneus RNA Prep Kit (for Plant)
Product No.

Size

08058-96

50 preps

Cica GeneusⓇ RNA Prep Kit (for Plant) provides an easy andrapid method for the isolation of total RNA such as leaves,stems and roots. It takes only 30 minute to remove various

Optional reagent

metabolites efficiently.

Product No.

Product Name

Size

11497-96

DNase I(For Cica Geneus® Prep Kit)

50 preps

The purified RNA is suitable for cDNA synthesis, RT-PCR,
northern blotting and etc.

Specifications
Protocol for Plant tissue
Grind fresh plant tissue(~100 mg) to afine powder quickly and completely,using amortar and pestle pre-cooledwith liquid nitrogen

Lyse1）Sample2）①Buffer RPL：350 μL

Sample size

100 mgMaximum loading volume

700 μL

Binding RNA capacity

100 μgElution volume

30~100 μL

Kit Contents
Contents name

Size

VortexGeneAll SV Column Type W

Incubate for 3 min（with collection tube）

Transfer the mixture
to the EzSep filter（with collection tube）

Centrifuge for 30 sec at 10,000×g
3) Transfer the mixture to new 1.5 mLtube
4）Add 70% Ethanol：same volume to 3)

Suspending (by pipette)

Bind
Transfer the mixture to the GeneAll SV Column Type W

Centrifuge for 30 sec at 10,000×g

Wash

EzSep filter

50 ×1
50×1

1.5 mL tube

100 ×1①Buffer RPL

25 mL×1②Buffer REL

25 mL×1③Buffer RBW

60 mL×1④Buffer RNW

60 mL×1⑤RNase-free water

15 mL×1⑥Buffer DRB

5 mL×1＊If you try DNase I treatment, please add DNase I (Product No.：11497-96)
to 70 μL of ⑥Buffer DRB before using .

Transfer to a new collection tube
5）③Buffer RBW：500 μL

Centrifuge for 30 sec at 10,000×g
OptionRNA purification from Spinach
RNA purified from Spinash using Cica GeneusⓇ RNA Prep Kit

Transfer to a new collection tube

(for Plant) and other company’ s products were resolved6）Diluted DNase Ⅰsolution：70 μL

on 1.0 % agarose gel.

Incubate for 10 min

M

1

2

3

4

7）③Buffer RBW：500 μL

Incubate for 2 minCentrifuge for 30 sec at 10,000×g
Transfer to a new collection tube
8）④Buffer RNW：500 μL

Centrifuge for 30 sec at 10,000×g

25S rRNATransfer to a new collection tube

18S rRNA

9）Repeat 8) onse

Elute

Place the column in a fresh 1.5 mL tube
10）⑤RNase-free water：50 μL

M：LadderLane 1,2：Cica GeneusⓇ RNA Prep Kit (for Plant)

13

Centrifuge for 1 min at 10,000×g

RNA solution

Lane 3,4：Other company’ s products

Related products 〜Cica Geneus® Extraction Reagent Series〜

prduct number

08178-96

prduct number

08210-96

prduct number

08199-96

prduct number

08211-96

Cica Geneus® Extraction is reagent for efficient extraction of genome DNA or total RNA
from cells.You can prepare PCR templete in simple operation (mix reagents and target
samples, Incubate)
Sample 10 μL
Reagent 100 μL

Preparetion of PCR templete only 10 minutes
soln. a 10 μL
soln. b 100 μL

Incubate

72℃―6 minutes
94℃―3 minutes

Collect supernatant
Product No.

■SampleStaphylococcus aureus ATCCR 6538TM■Target gene
16S rDNA (Ribosomal DNA)
■Culture condition
LB medium (30℃ 18h)

Product name

1 kbp ladder
negative control
positive control(purified DNA)
3~8 Extracted DNA
serial dilution
M
1
2

Package Size

08178-96

Cica Geneus Ⓡ DNA Extraction Reagent

1 Pack（120 rxn）

08210-96

Cica Geneus Ⓡ DNA Extraction Reagent ST

1 Pack（120 rxn）

08199-96

Cica Geneus Ⓡ DNA Extraction Reagent AN

1 Pack（120 rxn）

08211-96

Cica Geneus Ⓡ RNA Extraction Reagent

1 Pack（120 rxn）

14

Related products〜Enzyme for DNA amplification〜
phi 29 DNA Polymerase set● Apliﬁcation of genomicDNA form as little as a single cell
● DNA removed by original puriﬁcation method● Supressing non-speciﬁc ampliﬁcation

phi29 DNA Polymerase is the replicative polymerase fromphi29.
This polymerase is available for representative ampliﬁcation of the whole genome.

Fe a t ures

● Mesophilic enzymes● Exceptional strand displacement activity● Extreme processivity● Extreme extensibility● An inherent 3´→5' proofreading exonuclease activity

Example：Circular DNA amplification by RCA（Rolling Circle Amplification）
pUC 18 DNA was amplified with several kinds of phi29 DNA polymerase. Then size of pUC18 DNA (2.7 kbp) werecomfirmed after digesting amplificated DNA by restriction enzyme (BamH I and EcoR I).
（kbp）

pUC18 copy nunber
M 105 104 103 102

pUC18 copy nunber

Non-template（kbp）

M 105 104 103 102

pUC18 copy nunber

Non-template
（kbp）

4
3
2

4
3
2

4
3
2

1

1

1

Company Ａ

KANTO Reagent

M 105 104 103 102

Non-template

pUC18（2.7 kbp）

Company
B社製品 Ｂ● KANTO Reagent supress non-speciﬁc ampliﬁcation !
Product No.
10195-96

P r o d u c t

n a m ephi29 DNA Polymerase set（0.05 mg/ mL）

Store atSize-20〜-25℃

1set（25 μL × 4）

＊This product contains both phi29 DNAポリメラーゼ（0.05 mg/ mL）25 μL×4 and10 × Reaction Buffer 100 μL×4.
＊This product was a built jointly by KANTO CHEMICAL Co., inc and NARO(National Agriculture and Food Research Organization).

KANTO CHEMICAL CO., INC.
REAGENT DIVISION

East Muromachi Mitsui BLDG, 2-1, Nihonbashi Muromachi 2-chome,
Chuo-ku, Tokyo, 103-0022, JAPAN
Telephone+81-3-6214-1092
Telefax+81-3-3241-1053
http://www.kanto.co.jp
E-mail; kanto-61@gms.kanto.co.jp
2015. August.