The 25th Hot Spring Harbor International Symposium

Cutting Edge of Technical Innovations in Structural and Systems Biology
Medical Institute of Bioregulation, Kyushu University

November 13-14, 2015
Collabostation I, Hospital Campus, Kyushu University, Fukuoka, JAPANFriday, November 13, 2015
13:50 – 14:00 Opening Remarks: Hiroyuki Sasaki (Director of Medical Institute ofBioregulation)
Session 1:

Systems and Computational Biology

Chair: Yuji Sugita

14:00 – 14:20 Hiroyuki Kubota (Kyushu University, Japan)
Transomics analysis of acute insulin action: network reconstruction from multi-omics
data
14:20 – 14:40 Kazuhiro Aoki (Kyoto University)
Quantitative measurement of dissociation constant in living cell imaging
14:40 – 15:00 Yuji Sugita (Riken, Japan)
Free-energy calculations of conformational changes in membrane proteins bymulti-resolution methods
15:00 – 15:40 James C. Gumbart (Georgia Institute of Technology, USA)
Bridging the gap between X-ray crystallography and biological insight with MD
simulations
15:40 – 16:00 Mikita Suyama (Kyushu University, Japan)
Understanding genomic features from their structural aspects
16:00 – 16:20
Session 2:Coffee BreakSingle-Molecule Techniques

Chair: Toshio Ando

16:20 – 16:40
Masahiro Shirakawa (Kyoto University, Japan)
High-resolution imaging by using optically–detected magnetic resonance
16:40 – 17:00
Rikiya Watanabe (University of Tokyo, Japan)
Novel micro-device for highly sensitive analysis of membrane transporters
17:00 – 17:20
Toshio Ando (Kanazawa University, Japan)
Direct Visualization of Protein Molecules in Dynamic Action by High-speed AtomicForce Microscopy
17:20 – 18:00
Peter Hinterdorfer (Johannes Kepler University Linz, Austria)
Antibodies on the Video Screen: Bipedal Antibody Walking on Membranes observedwith High-Speed AFM
19:00 –Dinner party (by invitation only)Saturday, November 14, 2015
Session 3:Crystallography and NMR

Chair: Kosuke Morikawa

10:00 – 10:20 Tomoya Tsukazaki (NAIST, Japan)
Structure of YidC reveals a mechanism of Sec-independent 	  membrane protein
insertion
10:20 – 10:40 Daisuke Kohda (Kyushu University)
Rational design of crystal contact-free space in protein crystals for analyzing
main-chain dynamics of proteins
10:40 – 11:20 Ilme Schlichting (Max-Planck Institute, Germany)
Protein structure and dynamics studied with X-ray free-electron lasers
11:20 – 11:40 Noritaka Nishida (University of Tokyo, Japan)
In-cell NMR observation of the biological events within living cells

11:40 – 11:50

Group Photos

11:50 – 13:20

Lunch

Session 4: Short-talk Session for Young Researchers Chair: Daisuke Kohda
13:20 – 15:00 Short talks of 6 minutes each (See the next page for details)
15:00 – 15:20
Session 5:Coffee BreakElectron Microscopy

Chair: Kouta Mayanagi

15:20 – 15:40 Keisuke Ohta (Kurume University, Japan)
Entire membrane organization of mitochondria and surrounding endoplasmic reticulum
revealed by scanning electron microscope based three-dimensional reconstruction.
15:40 – 16:00Kouta Mayanagi (Kyushu University, Japan)
Single particle analysis of DNA replication fork complex.
16:00 – 16:20 Florence Tama (Nagoya University, Japan)
Hybrid approaches to characterize structure and dynamics of biomolecular systems
from single molecule experiments
16:20 – 17:00
Yifan Cheng (University of California, USA)
Structure of TRP channels by single particle cryo-EM, from blob-ology to atomicstructures
17:00 – 17:10

Closing Remarks: Kosuke Morikawa (Kyoto University, Japan)

Session 4: Short-talk Session for Young Researchers Chair: Daisuke Kohda
Saturday, November 14, 201513:20 – 15:00

Short talks of 6 minutes each

Y-01: Hiroaki Ohishi (Epigenomics and Development)
DNA Methylation Dependent- and Independent-Regulation of Monoallelic Expression
Maintained by Zfp57 in Ground-State Embryonic Stem Cells
Y-02: Hiroki Goto (Cancer Genetics)
Regulation of chondrogenesis and osteogenesis by Hippo pathway
Y-03: Chika Sakamoto (Molecular and Clinical Genetics)
Gene transfer of reprograming genes with newly developed non-transmissible measles virus
vector into hematopoietic cells
Y-04: Tsunaki Higa (Molecular and Cellular Biology)
p57 maintains epithelial homeostasis via regulation of quiescent intestinal stem cells
Y-05: Yasuo Takashima (Organogenesis and Regeneration)
Lin28b-mediated microRNA regulation in mouse hepatoblasts
Y-06: Hirotada Tajiri (Immunogenetics）
DOCK1 as a novel molecular target for controlling cancer invasion and metastasis
Y-07: Nona Abolhassani (Neurofunctional Genomics）
Integrative analysis obtained from three different approaches demonstrates insulin signalling
impairment associated with AEBP1 up-regulation in AD brains; The Hisayama Study
Y-08: Yuki Akieda (Cell Regulation Systems）
Zebrafish larval tissues possess an immune cell-independent defense system against cancer
Y-09: Kohei Yamada (Genomics)
Genetic Analysis for Benign Adult Familial Myoclonic Epilepsy using Linkage-Assisted ExomeSequencing.
Y-10: Yoshihiro Izumi (Metabolomics）
Next-generation metabolomics in the medical sciences
Y-11: Shinsuke Uda (Integrated Omics）
Estimation method of sparse partial correlation matrix for transomics network
Y-12: Yuki Kawasaki (Structural Biology）
Three-dimensional reconstruction of the AglB protein embedded in nanodiscs by negative
staining electron microscopy
Y-13: Daisuke Saito (Bioinformatics）
Linkage disequilibrium analysis of allelic heterogeneity in DNA methylation
Y-14: Tesshin Murakami (Host Defense）
Two types of IL-17A-producing γδ T cells play critical roles in protection against pulmonary
infection with Klebsiella pneumoniae in different activation manner
Y-15: Rieko Doi (Molecular Immunology)
Efficient adjuvanticity induced by a dual ligand for TLR and iNKT TCR
Y-16: Yuuta Imoto (Organelle Homeostasis)
Ultrastructure of Ring-shaped Supramolecular Nanomachinery for the Division of Peroxisome
and Mitochondrion Revealed by EM-Based Single Organelle Analysis