Ready. Set. Grow!
Cell culture products
by SARSTEDT2

SARSTEDT quality seal for cell andtissue culture products
ance with quality standards for cell and tissue culture is necessary to ensure that experiments are comparable andreproducible.

TC Tested

Cryo Performance Tested

TestedSterile/ DNA-/ DNase-/
RNase-/ Pyrogen-free/
non-cytotoxic

In accordance with our basic principle that products whichcome into contact with cells must not have a disruptive effecton the cells, these products are produced under the strictest
cleanroom conditions and are labelled with the ‘TC Tested’
quality logo.

Cr

C

In order to meet these requirements, for more than 25 years
SARSTEDT has been producing a wide range of certified
consumables for working with cell and tissue cultures.

Performan
yo Tested ceSince 1990, SARSTEDT has been offering its
customers high-quality cell culture products
produced in cleanroom conditions by trained
personnel, wearing protective clothing andusing automated production processes.

T

Come Grow With Us – these days, cell and tissue cultures are
not just used in basic research but, increasingly, also inapplied biotechnology as well as in clinical and pharmaceuticalresearch. Products of the highest possible purity and quality
are required for toxicity tests, quality controls of biochemical
processes, industrial production systems (e.g. production of
monoclonal antibodies) and many more applications. Compli-

During ‘vital preservation’ in CryoPure tubes,
CE/ IVD/ Sterile/
Pyrogen-/ DNA-/ DNase-/cell and tissue samples must not be exposed to RNase-free/
non-cytotoxic/
non-mutagenic
additional risks in terms of contamination with
disruptive substances. Sarstedt CryoPure tubes
are therefore subjected to a number of tests and, after passing
the defined examinations, are certified as follows:
We guarantee compliance with the following limits:■ Sterile
Based on ISO 11137

We guarantee compliance with the following limits:■ Pyrogen-free/endotoxin-free<0,06 EU/ml■ Sterility validated according to the ISO 11137 seriesof standards■ Non-cytotoxic
In compliance with ISO 10993-5■ Pyrogens/endotoxins <0.06 EU/ml■ Non-mutagenic
Proof of assessment of mutagen-free status was conductedaccording to Ames Test II■ Non-cytotoxic in accordance with the ISO 10993 seriesof standards■ Human DNA <0.5 pg/µl■ Bacterial DNA <0.02 pg/µl■ DNA-free
Human DNA <0.5 pg/μl, bacterial DNA <0.02 pg/μl■ DNase <7.1 x 10–5 U/µl

■ DNase/RNase-free
DNase <1x10–5 U/μl, RNase <1x10–9 Kunitz units/μl■ RNase <1.4 x 10–10 Kunitz/µl■

Try it out for free andwith no obligation!!
cellculture.
sarstedt.com/en

Ready. Set. Grow!  ///  SARSTEDT3

Growth surfaces andcolour coding
A basic requirement for the successful cultivation of cells
in-vitro is to simulate the in vivo environment of the relevant
cell type as accurately as possible. The surface condition of
the culture vessel is particularly important because many cell
types can only survive, proliferate and differentiate following
successful adhesion. In order to meet the requirements for as

many different cell types as possible, SARSTEDT offers flasks,
dishes and plates with three different growth surfaces. To
ensure clear identification of the vessels even after they havebeen removed from the packaging, the products are labelled
as follows according to the SARSTEDT colour coding system:

SARSTEDT standard surface
for adherent cells
Hydrophilic groups are introduced to the surface via thespecial treatment of the polystyrene surface. This enables thebinding of cell surface proteins and thus allows the cells toadhere to the plastic surface. The hydrophilic standard growthsurface, which is coded red, therefore provides an optimum
culture substrate for many adherent cells.
a

SARSTEDT Cell+ surface
for difficult adherent cells
Primary cells, sensitive cell culture lines and cells which arecultivated under serum-reduced/serum-free conditions placeparticularly high demands on the surface of the cell culture
vessels. The yellow-coded Cell+ growth surface has beendeveloped specifically for such cells. Additional polar groups
are introduced to the hydrophilic surface via the special
treatment of the plastic surface. This leads to improved
imitation of the in vivo environment and therefore to theadhesion of difficult cells. Due to its properties, the Cell+surface can make the use of coated culture vessels super­
fluous in many cases.

b

SARSTEDT suspension culture surface
Culture vessels with the green, hydrophobic growth surface
are ideal for suspension cells (usually cells of lymphoid origin,hybridoma cells, etc.) that are not adherently cultivated insolution. The hydrophobic surface minimises cell losses
caused by unwanted microadhesion during sub-cultivation.
c
100 µmThe cultivation of various cell types on SARSTEDT growth surfaces clearlyshows the vitality of the various cell types*. a) HEK293 cells cultivated on thestandard TC surface for 48 hours. b) CHO cells cultivated under serum-reduced
conditions (1%) on the Cell+ surface for 24 h. c) Jurkat cells cultivated on thesuspension cell surface for 72 h. The measuring bar corresponds to 100 µm.
* Our ‘Growth Surface References’ brochure (20.783) provides an overview ofcells that have been successfully cultivated on our various growth surfaces.

4

Cell culture basics

Ready. Set. Grow!  ///  SARSTEDTT

Cell culture flasks

Product characteristics of the
SARSTEDT cell culture flasks
The flask geometry has the following distinguishing
characteristics:■ All corners accessible with serological pipettes and cell
scrapers. (1)1■ High stability of the flasks, reducing the risk ofcontamination. In addition, the stacking edge that has been
applied to the flasks allows flasks to be stored securely on
top of one another.
■ The optimised sloping neck of the flask and the anti-drip
edge allow the medium to be tilted easily whilst at the sametime reducing the risk of contamination due to spillage. (3)■ The lot no and expiry date are printed on each flask andpermit easy traceability after removal from the packaging.
(4)

TestedSterile/ DNA-/ DNase-/
RNase-/ Pyrogen-free/
non-cytotoxic

For cell culture, SARSTEDT offers flasks with a growth area of
25 cm2, 75 cm2 and 175 cm2. All cell culture flasks are made ofhigh-grade crystal-clear polystyrene which is processed into aflat growth surface that is ideal for microscopic observation.
All cell culture flasks are tested and certified according to the‘TC Tested’ quality seal (see p. 2).

■ Large labelling fields on both sides of the neck, together
with printed white scaling on one side and engraved scalingon the other, to facilitate work. (2)C2■ All SARSTEDT cell culture flasks are available withthree different growth surfaces and can be clearly
identified from the coloured lids:
Red = adherent cells
Yellow = difficult, adherent cells
Green = suspension cells
3

4

5

6

The quick-release cap is particularly user-friendly because
only a 1/3 turn is needed to close or open it. The ribbed
quick-release cap is available in two designs:■ The filter cap has a membrane with a pore size of 0.2 µm,
ensuring consistent, sterile gas exchange. At the sametime, the hydrophobic properties of the filter minimise therisk of contamination.
■ The two-position screw cap (= without filter) enables gastight sealing of the flasks in the closed position, whilst,
in the ventilation position, the cells can be cultivated withconsistent gas exchange (arrows point up and down).
A recognisable click confirms that the cap has been
secured in the ventilation position and will not fall off. A
gap in the ribbing, together with arrows on the cap, allow
for a simple haptic and visual check of the closure position
when working and when in the incubator.

open

stoppedclosedOrdering information
Growth surface [cm2]Cap

Recommended
working volume [ml]Max. volume [ml]Packaging
Bag/box83.391025

without filter

7

12.5

10/30083.3910.002

25

with filter

7

12.5

10/30083.391175

without filter

21

55

5/100

83.3911.002

75

with filter

21

55

5/100

83.3912175without filter

50

125

5/40

83.3912.002

175with filter

50

125

5/40

83.3910.300

25

without filter

7

12.5

10/30083.3910.302

25

with filter

7

12.5

10/30083.3911.300

75

without filter

21

55

5/100

83.3911.302

75

with filter

21

55

5/100

83.3912.300

175

without filter

50

125

5/40

83.3912.302

175with filter

50

125

5/40

83.3910.500

25

without filter

7

12.5

10/30083.3910.502

25with filter

7

12.5

10/30083.3911.500

75

without filter

21

55

5/100

83.3911.502

75with filter

21

55

5/100

83.3912.500

175

without filter

50

125

5/40

83.3912.502

175with filter

50

125

5/40

Order noColourcode**= adherent cells= difficult, adherent cells

Zubehör
Order noColourcode*

Cap

DesignPackaging bag/box

83.3990.025

without filter

for T 25

25/100, individual, sterile

83.3990.075

without filter

for T 75

25/100, individual, sterile

83.3990.175

without filter

for T 175

25/100, individual, sterile= suspension cells

Cell culture dishes

T

Ready. Set. Grow!  ///  SARSTEDTC

7

TestedSterile/ DNA-/ DNase-/
RNase-/ Pyrogen-free/
non-cytotoxic

For the cultivation of cells in cell culture dishes, SARSTEDT
offers 35 mm, 60 mm, 100 mm and 150 mm dishes thatare tested and certified according to the ‘TC Tested’ quality
seal (see p. 2). The dishes are produced from high-grade
crystal-clear polystyrene, meaning that a flat growth surface
of excellent transparency is produced, allowing visual inspection of cell growth.

Product characteristics of the
SARSTEDT cell culture dishes
The cell culture dishes have the following distinguishing
characteristics:■ The new SUREGrip is a raised, rough ring which runs
around the bottom part and permits secure and convenientgripping of both parts of the dish, even when stacked. (1)■ Automatically secure handling of the dish reduces therisk of contamination.

1■ Clearly visible and tactile arrows on the lid and dish enable
the two parts to be placed together correctly. (2)■ Continuous gas exchange and securely fitted lid areensured by lobes on the lid.
■ Distinct stacking rings in the lid and base allow for
secure stacking of several dishes.
■ For cloning experiments, SARSTEDT offers
35 mm and 60 mm diameter dishes with a grid. (3)■ For better traceability, even after removal from thepackaging, each dish is labelled using the colour code as
well as the lot no and expiry date. (4)2■ All cell culture dishes are available with three different
growth surfaces:Red= adherent cells
Yellow = difficult, adherent cells
Green = suspension cells

3

4

8

The cell culture dishes are packed in a bag with a re-sealable
mini-grip, which is closed with a tamper-evident seal until it isopened for the first time.

Ordering information
Diameter/height [mm]Growth surface [cm²]Grid

Recommended
working volume [ml]Packaging bag/case

83.3900

35/10

8

no

3

10/50083.3900.002

35/10

8

yes

3

10/50083.3901

60/15

21

no

5

10/50083.3901.002

60/15

21

yes

5

10/50083.3902100/2058

no13

10/50083.3903150/20

152

no

36

5/100

83.3900.300

35/10

8

no

3

10/50083.3901.300

60/15

21

no

5

10/50083.3902.300

100/2058

no13

10/30083.3903.300

150/20

152

no

36

5/100

83.3900.500

35/10

8

no

3

10/50083.3901.500

60/15

21

no

5

10/50083.3902.500

100/20

58

no

713

10/300Order no.

Color code**= adherent cells= difficult, adherent cells= suspension cells

Ready. Set. Grow!  ///  SARSTEDT

Cell scrapers
For easy and complete recovery ofadherent cells■ Ergonomic polystyrene handle with ribbed, non-slip
handpiece

Scraper position■ Cell-friendly blade made of a highly flexible, rubber-likematerial

Rotate the blade by¼ turn to reach the‘Lifter position’■ In all cell scrapers, the blade can easily be switched fromthe scraper to the lifter position■ Three sizes: S, M and L

Lifter position■ Individual sterile packaging, pyrogen-free/endotoxin-freeand non-cytotoxic

The graph shows that, in a comparison with the Trypsin
control, it was possible to detach the highest cell count per
ml when using the SARSTEDT cell scrapers (96%). With thecompetitor products (A, B, C and D), the cell yields were
between 62% and 84% in a comparison with the Trypsin
control. The quality of the cell scrapers was compared bycultivating cells under the same conditions and harvestingthem using the same technique. Cell vitality, on the other
hand, was approx. 95% for all cell scrapers.

Cell scrapers – comparison
Zellschaber - Vergleich
100
80

Cells/ml (%)

Comparison of the SARSTEDT cell scrapers
with four competitor cell scrapers:

60
40
20

Trypsin
Trypsincontrol
Kontrolle

SARSTEDTSARSTEDT

AA

BB
CC
Competitors
Wettbewerber

Ordering information, cell scraper
Order noDescription

83.3950

Cell scraper with
two-position blade,size S

83.3951

Cell scraper with
two-position blade,size M

83.3952

Cell scraper with
two-position blade,size LBlade
length [cm]

1.35

1.7

1.7

Handlelength [cm]

24.0

24.0

36.0

Packagingblisters/box

Field of application

1/100• Cell culture flasks: T-25
• Cell culture plates: 24-, 12-, 6-well• Cell culture dishes• Cell culture tubes

1/100• Cell culture flasks: T-75
• Cell culture plates: 12- and 6-well• Cell culture dishes:
35 x 10/60 x 15/100 x 20/150 x 20

1/100

• Cell culture flasks: T-175• Cell culture plates: 12- and 6-well• Cell culture dishes:
35 x 10/60 x 15/100 x 20/150 x 20
• Roller bottles

D D

9Cell culture plates

T

10

C

TestedSterile/ DNA-/ DNase-/
RNase-/ Pyrogen-free/
non-cytotoxic

For multiple cultivation on a medium to small scale, SARSTEDT
offers cell culture plates with 6, 12, 24, 48 and 96 wells. The
plates are produced from high-grade crystal-clear polystyrene
and both the wells and indeed the entire plate are exceptionally flat. The highly transparent base is suitable for microscopic measurements from below. All cell culture plates aretested and certified according to the ‘TC Tested’ quality seal
(see p. 2).

Product characteristics of
SARSTEDT cell culture plates
The external dimensions of the SARSTEDT cell culture plates
are based on ANSI/SLAS standard 1-2004: Microplates –Footprint Dimensions and can be used for analyses in device
holders with these dimensions. Further characteristics of theplates are as follows:■ For better traceability, even after removal fromthe packaging, each plate is labeled with the colour code
as well as the lot no and expiry date. (1)1■ In order to ensure quick guidance when filling the wells,
the wells are alphanumerically (2) labelled on the edge
and in the areas (3) between the wells.
■ Free-standing wells reduce the risk of contamination
when pipetting. (3) + (4)■ Non-slip side grids in the base make it easier to securely
grasp the entire plate. The transparent side walls of thebase enable visual inspection of the medium. (4)2■ Both air vents and condensation rings are integrated
into the lid; together, they ensure consistent gas exchange
while simultaneously minimising evaporation.
■ All cell culture plates are available with three different
growth surfaces:Red= adherent cells
Yellow = difficult, adherent cells
Green = suspension cells
34Ready. Set. Grow!  ///  SARSTEDT

11

Ordering information
Order no.

Colourcode*

Number of wells

Base shape

Growth surface
per well [cm2]

Working volume[ml]

Packaging
Blister pack/box

83.3920

6

8.87

4

1/50

83.3920.005

6

8.87

4

5/100

83.3921

12

3.65

2

1/50

83.3921.005

12

3.65

2

5/100

83.3922

24

1.82

1

1/50

83.3922.005

24

1.82

1

5/100

83.3923

48

0.64

0.5

1/50

83.3923.005

48

0.64

0.5

5/100

83.3924

96

0.29

0.2

1/50

83.3924.005

96

0.29

0.2

5/100

83.3925

96–

max. 0.31

1/50

83.3926

96–

max. 0.29

1/50

83.3920.300

6

8.87

4

1/50

83.3921.300

12

3.65

2

1/50

83.3922.300

24

1.82

1

1/50

83.3923.300

48

0.64

0.5

1/50

83.3924.300

96

0.29

0.2

1/50

83.3920.500

6

8.87

4

1/50

83.3921.500

12

3.65

2

1/50

83.3922.500

24

1.82

1

1/50

83.3923.500

48

0.64

0.5

1/50

83.3924.500

96

0.29

0.2

1/50

83.3925.500

96–max. 0.31

1/50

83.3926.500

96–max. 0.29

1/50*= adherent cells= difficult, adherent cells= suspension cells

12

BIOFLOAT™—
Spheroid culture
In many areas of biomedical research, in vitro models areindispensable. The most conventional form is the two-dimensional cell culture. Discrepancies often occur when transferring
the results to an entire organism. The aim of the three-dimensional cell culture is therefore to close this gap between the invitro and in vivo situation.

BIOFLOAT™ solves your challenges in thearea of spheroid cultures
Some challenging spheroid cultures have already beensuccessfully established using the BIOFLOAT™ cell culture
surface (e.g. spheroids from primary hepatocytes).

Spheroid cultures offer a simple and cost-effective variant of
3D cell culture. The cells form a three-dimensional cellularaggregate with pronounced cell-cell and cell-matrix contacts.

Advantages of spheroid culture■ Increased cell-cell contacts■ Pronounced extracellular matrix■ Improved in vitro model

Brain cells
Dental cells
Lung cells
Cardiac muscle cells

Liver cells
Epithelial cells

Pancreatic cells

Bone cells

Kidney cells

Endothelial cells

Stromal cells

Detailed list of tested
cell types / cell lines:
sarstedt.com/
biofloat-zt-en

Ready. Set. Grow!  ///  SARSTEDT

13

Nice and round—3D cell cultures with
BIOFLOAT™ cell culture plates
BIOFLOAT™­—Benefits at a glance

The reliable quality of the BIOFLOAT™ cell culture surface
enables the formation of perfect spheroids even for challenging cells. This also includes cells that do not form spheroids
on existing products.

■ Easy handling thanks to robust coating■ Defined and xeno-free composition for a safe cultivation
with high reproducibility■ Fast and reliable spheroid formation for better planning ofyour daily laboratory routine

Control

Lightly scratched

Heavily scratched

BIOFLOAT™

Day 8Competitor A

Day 4

BIOFLOAT™

Day 2Competitor A

Day 0

Fig.: 100 μl of a suspension of primary human hepatocyteswith a concentration of 25,000 cells/ml (equivalent to 2,500
cells/well) was seeded per well. After spheroid formation,
50 μl of medium was exchanged every 48-72 h.

Fig.: The well bottom was lightly scratched using a standard
pipette tip (once all around with moderate pressure) andheavily scratched (30 s with strong pressure). 200 μl of asuspension of 3T3 cells with a concentration of 30,000 cells/
ml (corresponding to 6,000 cells/well) was then seeded per
well.

The SARSTEDT BIOFLOAT™ plate is individually sterilepacked in an aluminium bag. It is also endotoxin-free andnon-cytotoxic.

Order information
Order no.
83.3925.400
83.3927.400

Name
Cell culture plate, 96-well,surface: BIOFLOAT™, round bottom
Cell culture plate, 384-well,surface: BIOFLOAT™, round bottom

Try it out for
free & withoutobligation!
sarstedt.com/
biofloat-en

Number of wells
96384

Bottom shape Packaging
1 pc./aluminium bag
4 Pcs./Inner carton
24 Pcs./Outer carton

14

TC-inserts

Ready. Set. Grow!  ///  SARSTEDTSARSTEDT TC (Tissue Culture) inserts are easy-to-use inserts
for TC plates. When used in combination with our TC plates,
the inserts form a 2-compartment cell culture system in which
the in vivo situation of cells can be simulated extremely well.
Our TC inserts are therefore suitable for performing many
complex experiments in cell and tissue culture:■ Transport, secretion and diffusion studies■ Migration experiments■ Cytotoxicity tests

15

Membrane properties
The TC inserts are provided with a PET (polyester) membrane
and are available in five different pore sizes (0.4 µm, 1 µm,
3 µm, 5 µm and 8 µm) and two optical properties (transparent
and translucent). Our PET membrane offers the following
advantages:■ Ultra-thin, high-quality track-etched PET membrane with adefined pore size (Fig. 2a).
■ Both translucent (higher pore density, cloudy) andtransparent membranes (lower pore density) have a moredefined pore density.

■ Co-cultures■ Transepithelial electrical resistance (TEER) measurements■ Primary cell cultures■ Optimal cell adhesion with two-side surface treatment(TC treated).
■ The chemical properties of the PET membrane minimise
the non-specific binding of molecules.

■ 3D cell cultures■ etc.
The exceptionally user-friendly design of the suspended
SARSTEDT TC inserts boasts the following characteristics:■ Durable casing made from highly transparent
polystyrene (PS).
■ Asymmetrical design for easy pipetting in the well (Fig. 1a).
■ Spacers prevent fluid from getting drawn up between theinsert and the well.
■ Lowered upper edge allows for optimal gas exchange(see Fig 1b).

ab

■ High resistance to chemicals for easy fixing and stainingof the cells.
■ Detached membranes remain flat for convenient further
processing and microscope examination (Fig. 2b).

General information on the field of
application of the SARSTEDT TC inserts:■ Membranes with small pore sizes (0.4 µm, 1 µm) aresuitable for applications in which the migration of cells
through the membrane pores is undesirable. In co-culture
experiments, for example, cells can be cultivated in close
proximity to one another without the cell types becoming
mixed together.
■ Membranes with larger pores are recommended forexperiments in which the migration of cells through thepores to the underside of the membrane must be possible.
Depending on the cell type, membranes with a pore sizeof 3 µm, 5 µm or 8 µm should be used for performing
chemotaxis, invasion and migration studies, etc.
■ Translucent membranes with a pore diameter of 0.4 µm
allow for optimal basolateral diffusion for transport,secretion, diffusion and cytotoxicity studies, due to the highpore density.
■ Translucent membranes are suitable for both electron
microscopy and TEER (transepithelial electrical resistance)experiments.
■ Transparent membranes can be used for both light andelectron microscopy.

Figure 1ab

8 µm
Figure 2

16

The TC inserts are compatible with the corresponding TC
plates (see pages 12 and 13). All designs are pyrogen-free/endotoxin-free, non-cytotoxic and available in sterile individual
packaging.

Ordering information
Working volume[ml]
Insert

Well

Packaging
Blister pack/
box

4.5

1–4

2.4 – 4.8

1 / 24

12

4.5

1–4

2.4 – 4.8

1 / 24transparent

11

4.5

1–4

2.4 – 4.8

1 / 242 x 106transluzent

9

4.5

1–4

2.4 – 4.8

1 / 245.0

6 x 105transluzent

10

4.5

1–4

2.4 – 4.8

1 / 24PET

8.0

2 x 105transluzent

11

4.5

1–4

2.4 – 4.8

1 / 2483.3931.040

PET

0.4

1 x 108transluzent

12

1.1

0.2–0.8

1.2 – 2.4

1 / 4883.3931.041

PET

0.4

2 x 106transparent

12

1.1

0.2 – 0.8 1.2 – 2.4

1 / 4883.3931.101

PET

1.0

2 x 106transparent

11

1.1

0.2 – 0.8 1.2 – 2.4

1 / 48PET

3.0

2 x 106transluzent

91.1

0.2 – 0.8 1.2 – 2.4

1 / 4883.3931.500

PET

5.0

6 x 105transluzent

10

1.1

0.2 – 0.8 1.2 – 2.4

1 / 4883.3931.800

PET

8.0

2 x 105transluzent

11

1.1

0.2 – 0.8 1.2 – 2.4

1 / 4883.3932.040

PET

0.4

1 x 108transluzent

12

0.3

0.1 – 0.4 0.8 – 1.6

1 / 4883.3932.041

PET

0.4

2 x 106transparent

12

0.3

0.1 – 0.4 0.8 – 1.6

1 / 48PET

1.0

2 x 106transparent

11

0.3

0.1 – 0.4 0.8 – 1.6

1 / 48PET

3.0

2 x 106transluzent

90.3

0.1 – 0.4 0.8 – 1.6

1 / 4883.3932.500

PET

5.0

6 x 105transluzent

10

0.3

0.1 – 0.4 0.8 – 1.6

1 / 4883.3932.800

PET

8.0

2 x 105transluzent

11

0.3

0.1 – 0.4 0.8 – 1.6

1 / 48

Membrane Pore
Pore density Optical promaterialdia. [µm] [Pores/cm2] perty

Membranethickness[µm]

Growth area[cm2]

83.3930.040

PET

0.4

1 x 108transluzent

12

83.3930.041

PET

0.4

2 x 106transparent

PET

1.0

2 x 106

PET

3.0

83.3930.500

PET

83.3930.800

Order no83.3930.101
83.3930.300

83.3931.300

83.3932.101
83.3932.300

Format6-well12-well

24-wellReady. Set. Grow!  ///  SARSTEDT

17

Coverslips for microscopy
Highly transparent coverslips forcell cultivation
Wherever adherent cells need to be cultivated in sterile
conditions, fixed, stained and subsequently placed under amicroscope on a small surface, SARSTEDT coverslips are yourfirst choice. The double-sided surface treatment and the good

optical quality of the modified plastic material make it easy towork with coverslips. All designs are certified as sterile,
pyrogen-free/endotoxin-free and non-cytotoxic.

The sterile coverslips can be used in various products for cell cultivation:
Diameter[mm]

Packagingunits/box

35 x 10 dish6-well plate

12-well plate24-well plate

Coverslips

25

200

+

+

83.1840.001

Coverslips

22

200

+

+

–
–

–
–

83.1840.002

Coverslips

13

200

+

+

+

+

Order noDescription

83.1840

Colour code18

lumox®

lumox® cell culture products are characterised by their thin,
gas-permeable film base. The gas permeability and short
diffusion paths ensure optimum gas exchange. The lumox® filmbase has very low autofluorescence in comparison withconventional polystyrene bases (Fig. 1) and also has a higher
light transmission in comparison with conventional polystyrene or glass bases (Fig. 2). The minimal autofluorescence andthe good light transmission of the lumox® film lead to consistently high sensitivity in assays and when using imaging andreader technologies. lumox® products enable a range ofapplications from normal cell culture through to the automated analysis of fluorescence-based cell assays.

lumox®—Benefits at a glance

Relative fluorescence [RFU]

Ready. Set. Grow!  ///  SARSTEDT

19

lumox® film base
Polystyrene base

Wavelength [nm]Fig. 1 Fluorescence measurement of the lumox® film and thepolystyrene base at 330 nm■ Minimal autofluorescence■ High transparency■ Gas-permeable film base

■ Ideal for microscopic analyses

Transmission [%]■ Optimal growthlumox® film base
Polystyrene base
Glass base

Wavelength [nm]Fig. 2 Light transmission measurement. Detection of low
signals, particularly possible with wavelengths of 200–300 nm

Cells simply grow better
The gas permeability of the film base of the lumox® products
offers numerous advantages. The cells grow directly at theborder between the gaseous and liquid phases, where theculture medium cannot act as a diffusion barrier. Exceptionallyshort diffusion paths ensure optimal gas exchange. This meansthat the cells are directly supplied with oxygen, while also
allowing metabolic waste products, such as CO2, to escape.

Fig. 3 Gas exchange via the lumox® film baseFig. 4 No gas exchange is possible in conventional cell culture
vessels via the polystyrene or glass bases

20lumox® dish—The gas-permeable
cell culture dishThe lumox® dish consists of a crystal-clear polystyrene coverand a polystyrene frame with a transparent base made ofgas-permeable, ultra-thin (25 µm) lumox® film. The lumox®
dish is available with a diameter of 35 mm and 50 mm. Bothhydrophilic or hydrophobic cultivation surfaces are available,
which means that both adherently growing cells and suspension cells can be cultivated in a lumox® dish. For further
analyses, such as electron microscopy, the film can be cut
out using a scalpel. The lumox® dish is certified as sterile,
pyrogen-free/endotoxin-free and non-cytotoxic.

Ordering information—lumox® dishØ/height[mm]

Growth surface[cm2]

Working
volume [ml]Packaging inner box/caselumox® dish 35

35/6

6.3

2.5

50/25094.6077.331

lumox® dish 35

35/6

6.3

2.5

50/25094.6077.305

lumox® dish 50

50/12

20.4

5–10

50/20094.6077.410

lumox® dish 50

50/12

20.4

5–10

50/200Order no.

Description

94.6077.333

Surfacelumox® multiwell—The multiwell plate withlow autofluorescencelumox® multiwell plates consist of a black polystyrene frame(standard dimensions) with a transparent base made from thethin (50 µm), gas-permeable lumox® film. The 24-well, 96-welland 384-well formats are available for selection. All designs
are certified as sterile, pyrogen-free/endotoxin-free andnon-cytotoxic.

Ordering information—lumox® multiwell
Growth surface
per well [cm2]

Working volume
per well [µl]Packaging bag/caselumox® multiwell, 24-well

1.90

500 – 1500

4

94.6110.024

lumox® multiwell, 24-well

1.90

500 – 150020

94.6000.024

lumox® multiwell, 96-well

0.34

25 – 3404

94.6120.096

lumox® multiwell, 96-well

0.34

25 – 34020

94.6000.034

lumox multiwell, 384-well

0.11

10 – 1304

94.6130.384

lumox® multiwell, 384-well

0.11

10 - 130

20

Order no.

Description

94.6000.014®

SurfaceReady. Set. Grow!  ///  SARSTEDT

x-well cell culture chambers

x-well glass—detachable

The x-well cell culture chambers enable the cultivation andanalysis of cells on a slide. The slides form a single or multi-chamber vessel together with a polystyrene attachment.
Irrespective of whether you are performing fluorescence orlight microscopy analyses on living or fixed cells, individual
examinations or parallel test series, our comprehensive x-well
product range provides the ideal solutions for your applications. All products are certified as sterile, pyrogen-free/endotoxin-free and non-cytotoxic.

The standard format glass slide combines ideal growth
conditions for cells with outstanding optical properties.
The high chemical resistance also permits the use of mostfixatives and dyes.

■ Time-efficient execution of histological and fluorescencestaining■ Small compartments for the cost-efficient performanceof experiments

21■ Slide in standard format with labelling field■ Minimal autofluorescence■ Chamber can be detached from the slide without using
a tool■ Optimal 400-fold magnification (40x lens)

x-well coverglass■ Slides with outstanding optical properties

The x-well coverglass cell culture chambers have a basethickness of 170 µm and are therefore particularly well suitedfor high-resolution and confocal microscopy.

■ Cultivation of adherent cells■ High chemical resistance■ Minimal autofluorescence■ Slide in the short format without labelling field■ Slide not detachable■ Optimal 1,000-fold magnification (100x lens)

x-well lumox®—detachable
The growth surface of the lumox® x-well specimen slide ismade of gas-permeable lumox® film. Due to the outstanding
optical properties of the film base, x-well lumox® is ideal for
fluorescence-based cell analyses.

x-well PCA—detachable
The slide of the x-well PCA cell culture chambers is made ofa plastic from the polyolefin family and has the advantage
of a lower autofluorescence and higher chemical resistance incomparison with polystyrene.

■ Slides with thin lumox® film (50 µm) in standard format
with labelling field■ Minimal autofluorescence and high transparency■ Slide in standard format with labelling field■ Chamber can be detached from the slide without using
a tool■ PCA only has a low autofluorescence■ Optimal 400-fold magnification (40x lens)■ Chamber can be detached from the slide without using
a tool■ Optimal 400-fold magnification (40x lens)Ordering information—x-wellFormatPCA

lumox®

Glass

Coverglass

Growth area [cm2]

Working volume
per well [ml]Packaging
Blister pack/box

1 Well

94.6140.102

94.6150.101

94.6170.102

94.6190.102

9

4

6 / 96

2 Well

94.6140.202

94.6150.201

94.6170.202

94.6190.202

4.4

2

6 / 96

4 Well

94.6140.402

94.6150.401

94.6170.402

94.6190.402

1.9

16 / 96

8 Well

94.6140.802

94.6150.801

94.6170.802

94.6190.802

0.8

0.5

6 / 96

Flasche94.6140.002-

94.6170.002

94.6190.002

9

4

6 / 96

22

flexiPERM®—reusable cell culture
insertflexiPERM® is a reusable silicone insert which subdivides cell
culture vessels and slides into smaller cultivation units. The
highly adhesive bottom of flexiPERM® sticks to all flat surfaces,such as glass, plastic or flexiPERM® film.
■ flexiPERM® are adhesive, reusable silicone cell culture
chambers■ flexiPERM® are hydrophobic and non-toxic for tissue■ flexiPERM® cell culture inserts are heat resistant (up to
125°C), cold resistant (down to –20°C) and resistant toalmost all laboratory chemicals■ Can be sterilised by autoclaving or 70% ethanol■ flexiPERM are suitable for DIN slides and cell culture
dishes®

■ flexiPERM® cell culture inserts can be used for long-term
tests which last around 2 weeks

flexiPERM® slide and flexiPERM® micro 12
flexiPERM® slide (2) with eight and flexiPERM® micro12 (1)with twelve subdivisions are suitable for parallel analyses ofcells on DIN slides. In addition, they can be used with orwithout a slide in combination with quadriPERM®.

flexiPERM® conA and conB
The models flexiPERM® conA (3) and flexiPERM® conB (4)were developed for special cell examinations in animal andplant physiology.
The cone-shape form can be used for numerous applications
in micromanipulation/microinjection. Intracellular andintercellular measurements can be performed in simultaneous
microscopic observation.

flexiPERM® disc
The flexiPERM® disc (5) which has been subdivided into four
compartments is the ideal insert for the gas-permeable
lumox® dish 50 or any cell culture dish with a diameter of
50 mm.
The flexiPERM® disc can be used for co-cultivation of various
cell types in one vessel.

5

1
2

4
3

Ordering information—flexiPERM®
Order noDescription

Fig.

Culture units

Growth area per
sub-division [cm2]

Working volume[µl]

Packagingunits/box

94.6011.436

flexiPERM® micro 12

1

12

0.3

100 – 200

594.6032.039

flexiPERM® slide

2

8

0.9

300 – 500594.6077.435

flexiPERM conB

4

1

3.1

2,000 – 3,000

594.6077.434

flexiPERM® conA

3

1

1.1

1,000 – 1,500

594.6034.067

flexiPERM® disc

5

4

1.8

500 – 1,000

5®

Ready. Set. Grow!  ///  SARSTEDT

23

quadriPERM®—cell culture dishfor parallel analyses
quadriPERM® is a versatile rectangular cell culture dish that is
impressive on account of the following benefits:

Cell culture dish for parallel analyses
quadriPERM® has four compartments of identical size forparallel cell cultivation under the same conditions. Suspension
cells can be cultivated directly in the quadriPERM®. The
x-well products, flexiPERM® or DIN slides can be placed in thecompartments for the cultivation of adherent cells.

Versatile applications
Besides cell cultivation, the quadriPERM® can also be used fora wide range of different applications. The cell culture dish canbe used for in-situ preparations of chromosomes for cytogenetic studies. Fixation and histological, immunocytochemical
or immunofluorescence staining are also possible. In addition,
quadriPERM® can even be used as a multi-purpose vessel for
denaturation, hybridisation or membrane washing. Other
applications include:■ Parallel analyses

Easy handling■ Incubation of slides

In the quadriPERM®, the cells can be easily and swiftly
supplied with new medium. In addition, the outside dimensionsof a quadriPERM® dish comply with the ANSI/SLAS (formerly
ANSI/SBS) standard, meaning that quadriPERM® dishes,
like all SARSTEDT TC dishes, can easily be microscopicallyexamined.

■ Immunohistology■ Immunocytochemistry■ Fluorescence in-situ hybridisation (FISH)■ Cell microarrays■ Mycoplasma tests■ Northern, southern or western blot

Certified quality
quadriPERM® dishes are certified sterile, pyrogen-free/endotoxin-free and non-cytotoxic.

Ordering information—quadriPERM®
Order noDescription

Cultivation surface per unit [cm2]

Working volume per unit [ml]Packaging
Bag/box94.6077.307

quadriPERM®

24.9

approx. 10

12/48

quadriPERM® in combination
with flexiPERM® slideand flexiPERM® micro 12

24

miniPERM® bioreaktor

Ready. Set. Grow!  ///  SARSTEDTThe miniPERM® is an easy-to-handle bioreactor which wasdeveloped for the cultivation of eukaryotic cells (mammalian,
insect and plant cells) in high density and hence for biomass
production as well as for the production of cell products. The
sub-division of the bioreactor into production and nutrient
modules, together with rotating cultivation, enables the
production of highly concentrated cell products in small
volumes. Therefore, depending on the cell line, cell densities ofmore than 107 cells/ml and product concentrations of several
mg/ml can be achieved. This means that the miniPERM®
bioreactor is a cost-effective and time-saving alternative toconventional cell culture and roller bottles and to fermentation
systems.

25

The miniPERM® bioreactors are suitable for awide range of applications*, such as:■ Cultivation of hybridoma cells for producing antibodies■ Cultivation of transfected cells for producing recombinant
proteins or for virus production■ Biomass products of eukaryotic and prokaryotic cells

Advantages of the miniPERM® bioreactor:■ High cell densities■ High product concentrations■ Easy handling■ Multiple harvests■ The production module is available
in various sizes* References:
Belin, V., Rousselle, P., Production of a recombinantly expressed laminin
fragment by HEK293-EBNA cells cultured in suspension in a dialysis-based
bioreactor, Protein Expression & Purification, 48: 43–48 (2006)
Konstantinov, S. et al., Three-Dimensional Bioreactor Cultures: A Useful
Dynamic Model for the Study of Cellular Interactions, Ann. N. Y. Acad. Sci.
1030: 103–115 (2004)
Further references and user reports are available upon request.

26

The principle
The miniPERM® bioreactor is subdivided into a production
module and a nutrient module (two-compartment system) by adialysis membrane. The dialysis membrane has a cut-off sizeof 12.5 kDa, so that neither cells nor secreted cell products(> 12.5 kDa) can diffuse into the nutrient module. At the sametime, the exchange of nutrients and cell metabolites takes
place via the dialysis membrane. Gas exchange is effected via
a thin, gas-permeable silicone membrane on the outwardfacing side of the production module.

Nutrient module

Production module

Cell product
Glucose
Lactate

NH4+
CO2
Cell
O2

Silicone membrane
Dialysis membrane

miniPERM® production modules
The miniPERM® production modules are suitable for thecultivation of suspension cells. They are available in two
different culture volumes depending on the production scale:■ miniPERM® classic, with 35 ml cell culture volume, is theideal production unit for research laboratories.
■ miniPERM® HDC50, with 50 ml culture volume, is suitable
for the production of slightly larger protein and biomass
amounts.

The universal turning device
To achieve an optimal supply and disposal of the cells in theminiPERM® bioreactor, the cells are kept in suspension using
continuous rotation. During cultivation, the miniPERM®
bioreactor is rolled lying on its longitudinal axis on a universal
turning device in a CO2 incubator at a minimum of 70%
relative humidity.
The universal turning device accommodates for up to fourminiPERM® bioreactors.

Ready. Set. Grow!  ///  SARSTEDTThe bioreactor and accessories

miniPERM® accessories
The following accessories are available for easy handling of
the miniPERM® bioreactor:

miniPERM® bioreactors■ miniPERM® sterile:
The production and nutrient modules are certified sterile,
pyrogen-free/endotoxin-free and non-cytotoxic and aresupplied as a single-use bioreactors.

■ Sterile single-use syringes (2 ml, 50 ml)■ miniPERM® stand■ miniPERM® reusable:
The nutrient module is autoclavable and designed for
multiple use. The production modules are sterile, certified
pyrogen-free/endotoxin-free and non-cytotoxic and areavailable as individually packaged single-use products.

■ Sterile screw caps for the modules■ Sterile syringe needles (25G x 5/8”)■ Sterile filling tube
miniPERM® start-up support kit
This kit contains all the accessories required for starting theculture, sampling and harvesting.

Ordering information—miniPERM®
Order noDescription

Packaging units/box

94.6001.059

miniPERM® classic

Bioreactor, sterile294.6001.055

miniPERM® classic

Production module, sterile

494.6077.121

miniPERM HDC 50

Bioreactor, sterile294.6077.017

miniPERM® HDC 50

Production module, sterile

4®

Ordering information—accessories
Order noDescription

Packaging units/box

94.6001.153

Nutrient module for miniPERM , autoclavable

4

94.6001.054

Stands for miniPERM®

4

94.6001.036

Screw caps for production module, sterile

694.6077.037

Screw cap for nutrient module, sterile

16

94.6077.135

Luer syringe needle, 25G x 5/8“, sterile100

94.6077.136

Single use 2 ml Luer syringe, sterile100

94.6077.137

Single use 50 ml Luer lock syringe, sterile60

94.6077.138

Filling tube 5“, Luer, sterile50

94.6001.094

Start-up support kit

Quantity• Single use 50 ml Luer lock syringe, sterile8• Single use 2 ml Luer syringe, sterile20• Filling tube 5“, Luer, sterile8• Luer needle, 25G x 5/8“, sterile20• Septum seal, sterile6• Stand for miniPERM®

1

®

1

Ordering information—universal turning device
Order noDescription

Packaging units/box

94.6001.061

Universal Turning Device 115 / 230 V

1

27

28

CryoPure
freezing system

Ready. Set. Grow!  ///  SARSTEDT

29

Versatile design■ Sterile
Based on ISO 11137■ CryoPure tubes with internal thread and silicone O-ringare available with a volume of 2 ml to increase the storage
density (10 x 10 grid). (1)■ Non-cytotoxic
In compliance with ISO 10993-5■ CryoPure tubes with external thread and a volume of
1.2 ml to 5 ml in order to reduce the risk of contamination.
(1)

Performan
yo Tested ce■ Pyrogen-free/endotoxin-free<0,06 EU/ml

Cr

SARSTEDT‘s CryoPure vessels for vitalpreservation are tested and certified
for the protection of the cell material(see also p. 2):

CE/ IVD/ Sterile/
Pyrogen-/ DNA-/ DNase-/
RNase-free/ non-cytotoxic/
non-mutagenic

Inspirational ergonomics
The QuickSeal sealing mechanism enables ergonomic andsecure opening and closing of both types of cap with just
one turn. (2)■ Non-mutagenic
Proof of assessment of mutagen-free status was
conducted according to Ames Test II

Exceptional versatility■ DNA-free
Human DNA <0.5 pg/μl, bacterial DNA <0.02 pg/μl

The combination of 6 different cap colours with 6 different
cap insert colours provides up to 36 colour-coding options forvisual coding and easy sample identification.

■ DNase/RNase-free
DNase <1x10–5 U/μl, RNase <1x10–9 Kunitz units/μl■

Optimum design■ The optimum internal contour of the base of the CryoPure
tube facilitates residue-free sampling.

For the storage of cell materials and their components at
temperatures as low as –196°C, SARSTEDT offers aprofessional storage system with a wide range of highly
transparent CryoPure tube products.

■ Free-standing design. (3)■ The base of the CryoPure tubes allows the convenient
single-hand handling of the tubes in CryoRack 40 and mostother conventional work stands. (4)

1

2

3

4

30

CryoPure 1.2 ml tubes with external thread
Order noScrew cap

Nominal volume

72.377White

1.0 ml72.377.002

Red

1.0 ml72.377.004

Yellow

1.0 ml72.377.005

Green

1.0 ml72.377.007

Violet

1.0 ml72.377.992

Colour mix

1.0 ml

Packaging50/bag500/inner box
2,000/box

CryoPure 2.0 ml tubes with external thread
72.379White

1.8 ml72.379.002

Red

1.8 ml72.379.004

Yellow

1.8 ml72.379.005

Green

1.8 ml72.379.006

Blue

1.8 ml72.379.007

Violet

1.8 ml72.379.992

Colour mix

1.8 ml50/bag500/inner box
2,000/box

CryoPure 5.0 ml tubes with external thread
72.383White

4.5 ml72.383.002

Red

4.5 ml72.383.004

Yellow

4.5 ml72.383.005

Green

4.5 ml72.383.007

Violet

4.5 ml72.383.992

Colour mix

4.5 ml25/bag250/inner box
1,000/box

CryoPure 2.0 ml tubes with internal thread and silicone O-ring
72.380White

1.6 ml72.380.002

Red

1.6 ml72.380.004

Yellow

1.6 ml72.380.005

Green

1.6 ml72.380.006

Blue

1.6 ml72.380.007

Violet

1.6 ml72.380.992

Colour mix

1.6 ml50/bag500/inner box
2,000/box

Ready. Set. Grow!  ///  SARSTEDT

Ordering information—Colour-coding inserts for CryoPure tubes
Order noColourPackaging

65.386

White

100/bag · 3,000/box

65.386.002

Red

100/bag · 3,000/box

65.386.004

Yellow

100/bag · 3,000/box

65.386.005

Green

100/bag · 3,000/box

65.386.006

Blue

100/bag · 3,000/box

65.386.007

Violet100/bag · 3,000/box

65.386.992

Colour mix

100/bag · 5 colours · 2,500/box

CryoRack 40/work rack
■ 4 rows of 10 wells for a total of 40 CryoPure
tubes■ Single-handed operation due to base locking■ Coloured alphanumeric coding for easy sample
assignment■ Slip-proof rubber feet

Ordering information—CryoRack 40
Order noPackaging

93.856.040

1/bag · 10/box

31

32

Cryo boxes for low-temperature storage■ High-quality polycarbonate storage boxes for storage attemperatures down to –196°C■ Numerical coding for each tube in the box enables quick
sample identification■ Crystal clear lid and coloured base with large apertures forquick ventilation■ Full range of box designs for customary 1.2/2.0 and 5.0 ml
CryoPure tubes■ Flexible storage capacities with box formats 5 x 5, 9 x 9 and
10 x 10■ Autoclavable (121 °C, 20 min.)Dimensions
Suitable for cryo tubeas

1.2 - 2.0 ml

1.2 - 2.0 ml

1.2 - 2.0 ml

3.5 - 5.0 ml

Format5x5

9x9

10 x 109x9Storage capacity

25

81

100

81

Box size (W x D x H) in mm

75 x 75 x 52

132 x 132 x 53

132 x 132 x 53

132 x 132 x 95

Internal thread

Internal and external thread

Internal and external thread
Ideal for tubes with

Ordering information—cryo boxes
Packaging

ColourOrdering code

5/bag · 20/box

93.872.225

93.873.281

93.874.210

93.875.281

5/bag · 20/box

93.872.425

93.873.481

93.874.410

93.875.481

5/bag · 20/box

93.872.625

93.873.681

93.874.610

93.875.681

Ready. Set. Grow!  ///  SARSTEDT

33

Cell strainers
The SARSTEDT cell strainers provide an innovative, fast andeasy-to-handle solution for producing single-cell suspensions(primary cell cultures, flow cytometry). They have a high-quality nylon strainer and are available in 40, 70 and 100 µm poresizes.

1

Product characteristics of the SARSTEDT
cell strainers
The following characteristics simplify the work processes
and reduce the risk of contamination:■ Quick identification of the pore size thanks tocolour-coded products – 40 μm (blue), 70 μm (white)and 100 μm (yellow). (1)■ The cell strainers are individually packed in sterile blister
packaging and can be conveniently removed thanks to theclosed-all-round design, including grip. This minimises therisk of inadvertently coming into contact with the filter. (2)2■ Four webs along the edge ensure continuous ventilation
during filtration. This minimises the risk that the cellstrainer will overflow, i.e. the ‘air lock’ effect. (3)■ The cell strainers are stackable. This enables the multistage filtration of cell suspensions, e.g. after organ
digestion or the production of primary cells. (4)■ The cell strainers are compatible with conventional 50 ml
centrifuge tubes. (5)

3

4

5

6

■ The adapter enables their use with a range of other tubes
with smaller diameters (15 ml, 5 ml, Ø 17 x 100 mm,
Ø 12 x 75 mm FACS tubes). (6)■ Cell strainers and adaptors are certified sterile, pyrogenfree/endotoxin-free and non-cytotoxic.

Ordering information – Zellsiebe
Order noDescription

Pore size [µm]Colour code

83.3945.040

Cell strainer, sterile40

1 / 5083.3945.070

Cell strainer, sterile70

1 / 5083.3945.100

Cell strainer, sterile100

1 / 5083.3945.999

Adaptor for cellstrainers––

Packaging blisters/box

1 / 2534

Filtropur V, Filtropur BT■ Filtropur V and Filtropur BT are available with three poresizes (0.45 µm, 0.22 µm and 0.1 µm).

The Filtropur product range is suitable for the filtration of
aqueous solutions (e.g. cell culture medium) and includes
filtration units for a very wide range of volumes. The filter
membranes are available with different pore sizes and cantherefore be used for a wide range of applications. The
Filtropur product range is characterised by its variability, cost
efficiency and rapidity:■ The 0.1 µm PES-membrane is used for the effective
prevention and removal of mycoplasmas from solutions.
■ Ergonomically shaped, stable, sterile collection bottles areavailable for volumes of 250 ml to 1,000 ml.
■ The PES membrane enables a high throughput while
simultaneously reducing the filtration time and ischaracterised by its low protein absorption.

Filtropur V and Filtropur BT vacuum filtration
Filtropur V and Filtropur BT were mainly developed for applications in the field of cell culture and are equipped with polyether sulphone (PES) membrane filters. These products are
therefore ideal for the cold sterilisation of cell culture media
and aqueous protein solutions.

■ Filtropur V and Filtropur BT are certified sterile, pyrogenfree/endotoxin-free and non-cytotoxic.

Vacuum filtration units*
Order noDescription

Filtration
volume [ml]Membranedia. [mm]Membrane/poresize

Packaging
Bag/box83.3940.501

Filtropur V 25

250

50

PES/0,22 µm

1/12

83.3941.500

Filtropur V 50

500

75

PES/0,45 µm

1/12

83.3941.501

Filtropur V 50

500

75

PES/0,22 µm

1/12

83.3941.502

Filtropur V 50

500

75

PES/0,1 µm

1/1283.3942.500

Filtropur V 100

1.000

91

PES/0,45 µm

1/12

83.3942.501

Filtropur V 100

1.000

91

PES/0,22 µm

1/12* Each filtration unit has a screw cap in sterile packaging for the collection vessel.

Flask top filter for storage vessels, outside diameter 45 mm max.
Order noDescription

Filtration
volume [ml]Membranedia. [mm]Membrane/poresize

Packaging
Bag/box83.3940.511

Filtropur BT 25

250

50

PES/0,22 µm

1/24

83.3941.510

Filtropur BT 50

500

75

PES/0,45 µm

1/24

83.3941.511

Filtropur BT 50

500

75

PES/0,22 µm

1/24

Filtration collection vessels for Filtropur
Order noDescription

83.3940.505
83.3941.505
83.3942.505

Auffanggefäß für
Filtropur

Filtration
volume [ml]DesignPackaging
Bag/box250

Mit aufgeschraubtem Deckel

1/24

500

Mit aufgeschraubtem Deckel

1/24

1.000

Mit aufgeschraubtem Deckel

1/24

Ready. Set. Grow!  ///  SARSTEDT

35

Filtropur S, Filtropur S plus
and Filtropur L
Filtropur S, Filtropur S plus and Filtropur L

Filtropur S and Filtropur S plus

Filtropur S, Filtropur S plus and Filtropur L are suitable forfiltering aqueous solutions and are characterised by thefollowing properties:

The Filtropur S and Filtropur S plus syringe filters are often
used for sterile filtration of cell culture media, cell culture
additives and buffers, as they reliably remove microorganisms
and particles from the solutions that need to be filtered. The
syringe filters are available with a pore size of 0.2 µm and0.45 µm.

■ Low protein adsorption and high flow rate thanks to the useof Filtropur membranes■ Low dead volume■ Sterile, pyrogen-free/endotoxin-free and non-cytotoxic■ Biocompatibility due to GF pre-filter, 100% free frombinding agents and membranes that are 100% free ofwetting agents

Filtropur L
The ready-to-use Filtropur L products have a polyethersulfone(PES) membrane with an integrated glass fibre (GF) pre-filter.
Filtropur L, when combined with a diaphragm pump, is suitablefor quick sterile filtration of cell culture media and aqueous
solutions with a volume of up to 10 l. Filtropur L filters are
optionally available with a Luer lock or a tube connector.

Ordering information—Filtropur
Order noDescription

Application

Membranedia. [mm]Membrane/pore size

Packaging
Bag/box83.1826

Filtropur S 0.45
syringe filters

Ultra-purification/clear filtration

28

PES /
0,45 µm

1/50, sterile

83.1826.001

Filtropur S 0.2
syringe filters

Sterile filtration

28

PES /
0,2 µm

1/50, sterile

83.1826.102

Filtropur S plus 0.2
syringe filters

Sterile filtration/to
increase the total filt- 28
ration volume

CA/GF /
0,2 µm

1/50, sterile

83.3944

Filtropur L 0.2 S*
Inlet: Tube connector

Sterile filtration

64

PES/GF /0,2 µm

1/50, sterile

83.3944.001

Filtropur L 0.2 LS*
Inlet: Luer lock

Sterile filtration

64

PES/GF /0,2 µm

1/50, sterile*Zur Druckfiltration

Ordering information—accessories
Ordering code

Description

Packaging

83.1850

Diaphragm pump with tube set, stainless steel sinker and tube adaptor for Filtropur L

1/box

36

Serological pipettes
Automatic-Sarpette®

Ready. Set. Grow!  ///  SARSTEDT

Serological pipettes■ Manufactured from crystal-clear polystyrene■ Larger pipetting volume due to negative graduations■ Variable working method due to reverse scaling■ Optimised mouthpieces for universal fit in the mostcommon pipetting aids
■ Guide ribs on the mouthpiece of the 25 ml pipettes ensure
a secure fit in the retaining adaptor of pipetting aids
■ Simple volume identification with international colour
code on each individual blister pack
■ Easy to open, anti-static packaging■ Available in individual sterile* packaging or in a bagof 25 units* Individually packaged pipettes are sterile and certified
pyrogen-free/endotoxin-free and non-cytotoxic.

Ordering information—serological pipettes 1 ml, 2 ml, 5 ml, 10 ml, 25 ml, 50 ml
Colour code

Packaging Unit/
bag/box

Order noTotal volume/graduations

Design86.1251.001

1 ml

1/100 ml

Plugged, ind. wrapped sterile

100/1,000

86.1251.025

1 ml

1/100 ml

Plugged, sterile, in 25 units

25/1,000

86.1252.001

2 ml

1/100 ml

Plugged, ind. wrapped sterile

100/1,000

86.1252.025

2 ml

1/100 ml

Plugged, sterile, in 25 units

25/1,000

86.1253.001

5 ml

1/10 ml

Plugged, ind. wrapped sterile

50/50086.1253.025

5 ml

1/10 ml

Plugged, sterile, in 25 units

25/50086.1254.001

10 ml

1/10 ml

Plugged, ind. wrapped sterile

50/50086.1254.025

10 ml

1/10 ml

Plugged, sterile, in 25 units

25/50086.1685.001

25 ml

2/10 ml

Plugged, ind. wrapped sterile

25/20086.1685.020

25 ml

2/10 ml

Plugged, sterile, in 20 units

20/20086.1256.001

50 ml

1/2 mlPlugged, ind. wrapped sterile

30/90

Aspiration pipette, polystyrene■ For aspirating liquids using a vacuum pump
■ Individually packaged in sterile paper/plastic peel
packaging■ Pyrogen-free/endotoxin-free and non-cytotoxic■ Without print, without cotton plugs

Ordering information, aspiration pipette
Order noTotal volume/graduations

DesignPackaging units/box

86.1252.011

2 ml/without graduation

Without plug and print, ind. wrapped, sterile

1/1,000

37

38

Automatic-Sarpette®
■ The ergonomically designed SARSTEDT AutomaticSarpette® enables convenient and fatigue-free working
even with prolonged pipetting.
■ Ergonomic design and optimal weight distribution■ One-handed operation for convenient working■ Users can choose from three different pump speeds■ Precise and responsive control of filling and dispensing
speed using two pipetting triggers■ All plastic and glass pipettes fit perfectly thanks to silicon
pipette holder with graduated steps■ A 50 ml pipette can be filled in under 10 seconds at
maximum motor power■ Low-noise pump motor for pleasant working■ NiMH battery with a service life of up to 8 hours■ LED display for monitoring battery charge

Ordering information—Automatic-Sarpette®
Order noDescription

Packaging

90.189.200

Automatic-Sarpette® incl. EU charger, table stand, wall bracket, 2 replacement filters(0.45 μm and 0.20 μm)

1 unit/box

90.189.202

Automatic-Sarpette® incl. GB charger, table stand, wall bracket, 2 replacement filters(0.45 μm and 0.20 μm)

1 unit/box

90.189.203

Automatic-Sarpette® incl. US charger, table stand, wall bracket, 2 replacement filters(0.45 μm and 0.20 μm)

1 unit/box

90.189.204

Automatic-Sarpette® incl. AUS charger, table stand, wall bracket, 2 replacement filters(0.45 μm and 0.20 μm)

1 unit/box

90.189.205

Automatic-Sarpette® incl. KR charger, table stand, wall bracket, 2 replacement filters(0.45 μm and 0.20 μm)

1 unit/box

90.189.220

Replacement filter for Automatic-Sarpette®, pore size: 0.45 μm

5 units/bag

90.189.221

Replacement filter for Automatic-Sarpette , pore size: 0.2 μm

5 units/bag

90.189.222

Silicone adaptor for Automatic Sarpette®

1 units/bag

90.189.223

Replacement batteries for Automatic-Sarpette®

2 units/bag®

Ready. Set. Grow!  ///  SARSTEDT

Conical tubes, 15 ml and 50 ml volume, sterile■ Exceptionally clear polypropylene (PP) enables unrestricted inspectionof the sample material■ Printed graduation and labelling field■ Graduation and labelling field are resistant to ethanol and methanol■ Can be centrifuged up to 20,000 x g* (exception: 62.559.001 only up to 8,000 x g)
■ Sterile, pyrogen-free/endotoxin-free, non-cytotoxic

Order noVolume [ml]Length [mm]Diameter[mm]Version

Packaging bag/box

62.559.001

50

115

28

with skirted base, red cap assembled

25/30062.547.004

50

114

28

Red cap, fitted

25/polystyrene rack/300

62.547.254

50

114

28

Red cap, fitted

25/30062.554.002

15

120

17

Red cap, fitted

50/polystyrene rack/500

62.554.502

15

120

17

Red cap, fitted

50/500* For liquid density of 1.06 g/ml and centrifugal inserts which are adapted to the conical tube base, tested at 20°C, for 30 minutes.

Cell culture tubes, crystal-clear PS, sterile
The pretreated polystyrene tubes with screw cap are particularly suitable for:■ Cultivation of small cell populations■ Cultivation of suspension or monolayer cultures■ The screw cap allows uniform aspiration and gas-tight sealing of the cells

Order noVolume [ml]Length [mm]Diameter[mm]Version

Packaging bag/box

83.9923.945

15

125

16

Red cap, TC-treated

5/1,000

83.9923.943

12

99

16

Red cap, TC-treated

5/1,000

83.9923.929

10

97

16

Red cap with skirted conical base, TC-treated

5/1,000

Tubes with two-position closure, sterile
The ventilation plug has a two-level seat. The first position (plug lightly fitted)
enables the ventilation of the inside of the tube. When securely pressed down, theplug tightly seals the tube in the second position.

Order noVolume[ml]

Length [mm]Diameter[mm]Version

Packaging bag/box

55.526.006 PP

5 ml

75 mm

12 mm

Without print

25/1,000

55.476.013 PS

5 ml

75 mm

12 mm

Without print

25/1,000

62.526.028 PP

5 ml

75 mm

12 mm

Printed graduationind. wrapped, sterile, 500/box

62.476.028 PS

5 ml

75 mm

12 mm

Printed graduationind. wrapped, sterile, 500/box

62.515.006 PP

13 ml

100 mm

16 mm

Printed graduation

25/50062.515.028 PP

13 ml

100 mm

16 mm

Printed graduationind. wrapped, sterile, 500/box

For further information on our tube range refer to our „Tube Finder“ at www.sarstedt.com.

39

If you have any questions:We’d be happy to help!

SARSTEDT AG & Co. KG
Sarstedtstraße 1D-51588 Nümbrecht
Phone: +49 2293 305 0
Fax:		 +49 2293 305 3992
export@sarstedt.com
www.sarstedt.com

BRO_20_215_0700_200_NF   This document may contain information on products that may not be available in particular countries   Technical modifications reserved

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