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00. Electrical Engineering.An investigation of keratinocyte melanogenesis in vitro by Fontana-Masson staining. Although themelanin content of skin cells is well documented and is used clinically as a marker for determining the depth of skinpigmentation, the structural and ultrastructural events involved in melanogenesis, especially in melanocytes, are not wellunderstood. We have used a melanogenesis assay in vitro with the following assay conditions: B16 mouse melanoma cells, 3T3mouse fibroblasts, and human keratinocytes. Melanin content was directly related to the concentration of melanin-inducing
agent (MSH) added, with the optimal concentration being 5 x 10(-7) M. Lysosomal and melanosomal morphology,
melanogenesis, and transferrin-iron uptake were investigated for cells treated with 50 nM--10 microM MSH for 5 days. Mitotic
rates in 3T3 and B16 cells were significantly reduced by > 60% by the addition of 5 x 10(-7) M MSH. Significant differences inlysosomal and melanosomal morphology were noted in B16 and human keratinocytes. In B16 cells and 3T3 cells treated with 5 x10(-7) M MSH, lysosomal and melanosomal morphology were qualitatively and quantitatively similar. Human keratinocyte
cytoplasmic melanogenesis was present only at the concentration of 10 microM MSH. All human and B16 cells demonstrated
melanin transferrin-iron uptake activity. Thus, keratinocyte lysosomes and melanosomes contain divalent metals and can take upiron that becomes incorporated in the melanin synthesizing enzyme tyrosinase.Q: What does 'Nós te dámos' mean in thisSpanish sentence? What does Nós te dámos mean in this Spanish sentence? f678ea9f9e
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