Quick GuideiQ-Check Free DNA Removal Solution, 3594970
For Use with Easy I and II Deep Well Extraction Protocols■

1 ml distilled water1 ml■

Enrich samples according to the protocol specific to the matrix/organism being tested

Rehydrate lyophilized G1 in 1 ml distilled water for 5–10 min at room temperature
DO NOT VORTEX

G1■

Transfer the 1 ml volume to vial G2, which contains activation buffer■

Gently mix by inversion■

Add 10 µl of activated G2 solution to the wells of a deep well plate

G2*10 µl100 µlNote: For the iQ-Check Vibrio protocol, add 20 µl of activated solution■

Add 100 µl of each sample

Avoid shaking the stomacher bag to prevent collecting large fragments of food debris
G2■

Seal the deep well plate using prepierced sealing film■

Incubate at 37 ± 2°C for 15–30 min in the thermoshaker without shaking■

Adjust the thermoshaker to 95–100°C

37 ± 2ºC*

15–30 min*100 µl■* Steps automated by iQ-Check Prep System.

Proceed with the Easy I or II iQ-Check extraction protocol by adding 100 µlof lysis reagent to the treated samples in the deep well plateOrdering Information
Catalog #3594970

12010806
35781353578137
12003068
357811435781243578113357812335781423578139357814012004306
12006574

Description
iQ-Check Free DNA Removal Solution, 1 vial
(175 mg lyophilized powder) iQ-Check Free DNA Removal
Reagent, 1 vial (9 ml) iQ-Check 10x Activation Buffer
iQ-Check Aspergillus Kit, 96 reactions
iQ-Check Campylobacter Kit, 96 reactions
iQ-Check Cronobacter Kit, 96 reactions
iQ-Check Enterobacteriaceae Kit, 96 reactions
iQ-Check E.coli O157:H7 Kit, 96 reactions
iQ-Check Listeria monocytogenes II Kit, 96 reactions
iQ-Check Listeria spp. Kit, 96 reactions
iQ-Check Salmonella II Kit, 96 reactions
iQ-Check S. Enteritidis Kit, 96 reactions
iQ-Check STEC VirX Kit, 96 reactions
iQ-Check STEC SerO Kit, 32 reactions
iQ-Check S. Typhimurium Kit, 96 reactions
iQ-Check Vibrio Kit, 96 reactions

Visit bio-rad.com/iqcheck for more information.
BIO-RAD is a trademark of Bio-Rad Laboratories, Inc. IQ-CHECK is a trademarkof Bio-Rad Europe GmbH in certain jurisdictions. All trademarks used herein are theproperty of their respective owner.

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