D-PLEX SMALL RNA-SEQ LIBRARY PREP KIT
Go deeper with your RNA research & Biomarker discovery

The Diagenode D-Plex Small RNA-seq Library Prep Kit is an optimal technology to study the small non-coding
RNAs (< 200 nucleotides) that play key roles in the regulation of gene expression at the transcriptional andpost-transcriptional levels. This unique ultra-sensitive, ligation-free method uses two innovative mechanisms- poly(A) tailing and template switching - to generate RNA libraries for next generation sequencing.

ADVANTAGES OF D-PLEX TECHNOLOGY•Ultra-low input capability: down to 10 pg for small RNA and 100pg for total RNA samples•

High library complexity for novel and comprehensive transcript detection•

Versatile integration into numerous transcriptomic analysis workflow: ribosome profiling, CLIP-seq, RIP-seq,…

•Improved quantification accuracy through the use of unique molecular identifiers (UMIs)•

Easy to use with minimal hands-on time: one-day, one-tube protocol

Maximize information from the regulatory transcriptome using D-Plex small RNA-seq kit for library preparationand MGcount*, quantification software developed at Diagenode, to analyze reads with less multi-alignment andmulti-overlaping ambiguity.

WORKFLOWThe D-Plex smRNA-seq is a versatile tool for aplethora of transcriptomic analysis

Bacterial andplants smRNA

Ribosome profiling

Genome-wide mappingof protein: RNA binding sites(CLIP, HITS-CLIP, RIP)

smRNA profiling inchallenging samples

piRNA profiling inseminal plasma

Schematic overview of the D-Plex small RNA-seq Library Prep workflow. RNA molecules are polyadenylated at the3’-end and primed with a oligo(dT) primer containing part of the ILMN 3’-adapter sequence. The addition of a templateswitching oligonucleotide during cDNA synthesis enables to fuse part of the ILMN 5’-adapter sequence during the reverse
transcription reaction.

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High library diversity for ultra-low inputs
A.

B.

A large number of features detected per biotype at CPM ≥5 in A) human brain RNA and B) mouse liver, using different starting quantities.

Accurate representation of the smRNA content of a sample
A.

B.

A) Accurate identification of PCR duplicates in low-input samples using the unique molecular identifiers (UMIs).
B) D-Plex (template switching) technology enables the recovery of the initial miRNA distribution of the miRXplore Universal Reference (Miltenyi BiotechInc., Cat. No. 130-093-521). In contrast, ligation-based kits display a strong distortion of the miRNA equimolar distribution initially present in the pool,indicating sample incorporation bias.

ORDERING INFORMATION
illumina sequencing platform
Library prep

D-Plex Small RNA-seq Kit

C05030001

24 rxns

Indexes

D-Plex UDI Module - Set A
D-Plex UDI Module - Set B
D-Plex UDI Module - Set C
D-Plex UDI Module - Set D

C05030021
C05030022
C05030023
C05030024

24 UDIs, 24 rxns

mgi sequencing platform
Library Prep

D-Plex Small RNA DNBSEQ Kit

C05030051

24 rxns

BarcodesD-Plex DNBSEQ Barcodes Module - Set A
D-Plex DNBSEQ Barcodes Module - Set A

C05030060
C05030061

24 barcodes, 24 rxns

*Hita, A., Brocart, G., Fernandez, A. et al. MGcount: a total RNA-seq quantification tool to address multi-mapping and multi-overlapping alignments
ambiguity in non-coding transcripts. BMC Bioinformatics 23, 39 (2022). https://doi.org/10.1186/s12859-021-04544-3

www.diagenode.com Please contact us for more information

Last update: September, 2023

Europe Diagenode sa / LIEGE SCIENCE PARK // Rue Bois Saint-Jean, 3 // 4102 Seraing (Ougrée) // Belgium // Phone: (+32) 4 364 20 50 // E-mail: info@diagenode.com
USA Diagenode LLC. / 400 Morris Avenue, Suite 101 // Denville, NJ 07834 // USA // Phone: (+1) 862 209-4680 // E-mail: info.na@diagenode.com