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5. Antifungal Potency Evaluation Test Methods for Antifungal Products(2014 Version)
1. Outline
These test methods shall be applied to antifungal potency tests for antifungal products conferred with anantifungal function (hereinafter referred to as products).
Detailed procedures shall be in accordance with the procedures specified for the individual test methods.
2. Choice of Test Method
Out of the test methods for antifungal products shown in the table below, an appropriate test method
befitting the subject material and intended use shall be chosen. Specifically, a test method allowing
fungal growth rated to be grade “1” or higher by the rating criteria shown in section 3 below shall beapplied.
Table. Test methods for antifungal products
Subject
Example subjects

Level
Plastics

Electrical/electronic products
A1Paints
Leather and leather products

Test method

JIS Z 2911 Appendix A:Method B
JIS Z 2911 Appendix B: Test None
Distilled water
Method 2
JIS Z 2911 8: Paints
Sabouraud medium Distilled water
JIS Z 2911 9: LeatherPDA
Distilled water

Plastics
BC

Culture medium
Spore suspension Pretreatment
usedGlucose and others Glucose and others None

JIS Z 2911 Appendix A:
Method A(Textiles)
JIS Z 2911 7:
Wet test method for textiles
Synthetic polymer materials
ASTM G21-09
Optical parts and equipment
JIS Z 2911 Appendix C
Products for general industrial
JIS Z 2911:
use
General industrial products(Textiles)
JIS Z 2911 7:
Dry test method for textiles
Electric and electronic products JIS Z 2911 Appendix B:
Test Method 1

SucroseOthers
None
None

Inorganic salts

Inorganic salts

None

Inorganic salts

Distilled water

None

Inorganic salts
None
None

Inorganic salts
Inorganic salts
Distilled water

None

None

Distilled water

None

None

Distilled water

None

None
None

Note：JIS Z 2911 be tested in 2010 version

1

If the mold growth rating for an untreated product is “0” even when determined by a level-A test method, thefollowing methods may be applied.
1) Add glucose to the spore suspension. (Applied mainly to leather and paints, or in cases where no growth isobserved by the test method of Appendix B)
2) Increase the dose of the spore suspension. (Applied mainly in cases where no growth is observed by the testmethod of Appendix A)3) The sample may be subjected to pretreatments such as water immersion, hot water immersion, and neutralization asrequired.

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3. Rating Criteria
Different procedures are specified for respective test methods. Whatever the test method chosen,
however, judgment shall be made using the following rating criteria. All these determinations shall bemade by visual examination.
Rating criteria
0: No hypha growth is observed in the inoculation portion of the sample or test piece.
1: The area of the hypha growth in the inoculation portion of the sample or test piece does not exceed one
third of the total area.
2: The area of the hypha growth in the inoculation portion of the sample or test piece exceeds one third of
the total area.
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