Mosaic 2.4
Instruction Manual

Copyright 2011-2022 Tucsen Photonics Co., Ltd.
All rights reserved.

Contents
General Introduction .............................................................................................................................. 4
1. Starting Interface ................................................................................................................................ 5
2. Windows .............................................................................................................................................. 7
3. Capture .............................................................................................................................................. 14
3.1 Capture .................................................................................................................................... 14
3.2 Resolution ................................................................................................................................ 14
3.3 Binning ..................................................................................................................................... 14
3.4 Exposure Control ..................................................................................................................... 15
3.5 White Balance ..........................................................................................................................16
3.6 Bit of Depth .............................................................................................................................. 17
3.7 Image Adjust ............................................................................................................................ 17
3.8 Histogram .................................................................................................................................20
3.9 Temperature Control (only for Cooling camera) ...................................................................... 21
3.10 File Save ................................................................................................................................ 22
3.11 ROI ......................................................................................................................................... 24
3.12 Mask ...................................................................................................................................... 25
3.13 Imaging Stitching(Live) .......................................................................................................... 26
3.14 EDF(Live) ...............................................................................................................................28
3.15 Realtime Dye ......................................................................................................................... 29
3.16 Video Record ......................................................................................................................... 29
3.17 Trigger (FL 20BW) ................................................................................................................. 31
3.18 Image Process (FL 20BW) .................................................................................................... 33
3.19 Delay Capture ........................................................................................................................34
3.20 Save Settings .........................................................................................................................36
3.21 Light Frequency ..................................................................................................................... 37
3.22 Other Settings ........................................................................................................................37
3.23 Auto Focus (only for Auto Focus camera) ............................................................................. 37
4. Image ................................................................................................................................................. 39
4.1 Image Adjust ............................................................................................................................ 40

Page 1

4.2 Image Dye ................................................................................................................................41
4.3 Fluorescence ........................................................................................................................... 42
4.4 Advanced Computational Imaging ...........................................................................................44
4.5 Binaryzation ............................................................................................................................. 46
4.6 Histogram .................................................................................................................................47
4.7 Smooth .....................................................................................................................................48
4.8 Filter/Extract/Inverse Color ...................................................................................................... 48
4.9 Deconvolution .......................................................................................................................... 49
4.10 Automatic Counting ................................................................................................................49
4.11 Automatic Counting Property ................................................................................................. 53
5. Measure ............................................................................................................................................. 55
5.1 Measure Tool ........................................................................................................................... 56
5.2 Calibration ................................................................................................................................59
5.3 Measure Layer .........................................................................................................................60
5.4 Graphics Properties ................................................................................................................. 61
5.5 Metrics Flow .............................................................................................................................62
5.6 Manual Class Counting ............................................................................................................65
5.7 Scale Property ......................................................................................................................... 67
5.8 Ruler Property ..........................................................................................................................68
5.9 Grid Property ............................................................................................................................69
5.10 Save Settings .........................................................................................................................70
5.11 Fluorescence Intensity ........................................................................................................... 71
5.12 Cursor Property ..................................................................................................................... 72
6. Report ................................................................................................................................................ 74
6.1 Template Report .......................................................................................................................74
6.2 Report ...................................................................................................................................... 76
7. Display ............................................................................................................................................... 78
8. Confi ...................................................................................................................................................79
8.1 Capture\Image\Measure .......................................................................................................... 79
8.2 Jpeg ......................................................................................................................................... 80
9. Info ..................................................................................................................................................... 81
9.1 Preferences ..............................................................................................................................81

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9.2 Help ..........................................................................................................................................82
9.3 About ........................................................................................................................................82
10. Customer Services ......................................................................................................................... 85

Page 3

General Introduction
Mosaic is a set of powerful software that integrates the controlling the working of
micro-imaging cameras, the calculating imaging, the images processing and theflowing work of measuring and counting, from it, users can obtain much better
operating experiences;
Mosaic is able to drive and control MIchrome series of Tucsen Cameras which areinstalled on the microscopes from different manufacturers, it helps to bring you thebest performance in your micro-imaging jobs.
When you need to make the best uses of the potentials of your microscopes and theircameras, Mosaic is able to apply accurately almost all the parameters you select, andit optimizes to these settings of these parameters through its user-friendly designs.
Mosaic enables users to set up their customized desktops according to their actual
applications. When user combine the customized desktops with his customized
working flows, there will be a distinct layout of the software working appearance, theuser is ensured the accuracy of his experiment results no matter the software is
working with multi-task or under multi-user circumstances.
Mosaic achieves senior quality images due to its powerful real-time calculating engine.
The function of real-time stitching helps you to obtain a super wide of FOV image
which is the result of real-time auto-images-stitching, when you moving yourmechanic stage of the microscope. Real time EDF functions is able to offer you a
combined picture of extended-depth-focusing(EDF) rapidly with less than one second.
From the users’ points of view, assuring the best operating procedures, thedevelopers of Mosaic refined the all-new camera operating work flow as Efficient
image acquisition→Image edition→measurement→report by modularization designs.
This work flow, acts in concert with the latest image processing algorithms of
TUCSEN, greatly saves the users’ operation time and then improves their working
efficiency.

Page 4

1. Starting Interface
When installing and starting up the software for the first time, the biological or industrial
application option box will pop out, select your application direction to enter thesoftware. The software will automatically optimize parameter settings based on yourchoice. While using software, the application direction can also be switched through[Information] - [Options] - [Microscope] on the right upper part of menu bar.

Figure 1-1

Figure 1-2

Click and select [Biological]. Entering the software, automatic white balance isdefaulted with gamma value 2.10 and the mode of exposure to the right. Click andselect [Industrial]. Entering software, the default color temperature value is 6500K,
and the software default as area white balance, and adopts the gamma value of 1.80
and middle exposure mode.

Page 5

Figure 1-3

It normally takes around 10 seconds for software start-up. Some cameras will open fora longer time, such as the FL-20BW will generally open for about 20 seconds.
If the camera is not connected or an unsupported camera is connected, the software
only supports image processing, measurement operations and report operations.

Figure 1-4

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2. Windows
The home screen of Mosaic software is mainly consisted of five parts - status bar,control bar, preview window, data bar, picture bar:

Figure 2-1① Status Bar
There are seven main modules in the status bar, they are Capture / Image / Measure/ Report / Camera List / Display / Config / Info. Click on the certain bar, the software
will switch to related interface.

Multi-camera connection and hot-plug function, only supported by Windows system,
not supported by Mac system, and please use computer's USB3.0 port for hot swap,
and do not unplug or plug the camera when the camera list is refreshed.
In the camera list, the recognized camera model is displayed. Click the camera nameto switch the camera.
When the current camera is removed, it will automatically switch to another camera, ordisplay no camera.

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② Control Bar
All function modules supported by camera are displayed on the left side, click module
status bar button to check detailed functions.
Clickbutton to unfold all the functions display. Clickbutton to fold up all thefunctions display.

Figure 2-2③ Preview Window

Page 8Figure 2-31) To display the real-time viewing and captured images;
2) Mouse roller can zoom out/zoom in pictures; the cursor always appears at thecenter of the picture.
3) Hold down the left button / right button / scroll wheel of mouse to drag the image
display area;
4) Click the control button at the edge of the window: left, right, down can expand or
contract the corresponding operating bar;5) Click the

button to save the currently selected picture as another document,

then the following window will pop up. The software supports four image formats
for saving or saving as: [JPG] [TIF][PNG][DICOM];

Page 9Figure 2-4④Data BarTo show the measurement and statistics tables

Figure 2-5

The measurement table supports the export of custom templates. For specificoperations, please refer to the report chapter.
⑤Image Bar
The captured pictures, videos and pictures, video thumbnails opened through otherpaths are displayed on the right side of the software. Picture shows emission
reduction by time from top to bottom, to click any image can switch it to [Image] pagein order to adjust the parameter settings. The specific operation is as follows:
1) Click the

button to locate the saving path of the file, select the path in which

the image need to import or open, the interface will switch to the following
To double click selected pictures can add them to the preview window on the rightside.

Page 10Figure 2-62) Right click on one of the image, the dialog box of operations will pop up as below.

Figure 2-7

It supports [Select All (A)], [Cancel All], [Open], [New Folder (F)], [Copy (C)],[Paste (V)], [Delete (D)] and [Rename (M)]; you can use [ctrl+c] shortcut keys tocopy images, use [ctrl+v] to paste it to the current folder; Select the files-saving
path on the left side. Click thebutton to close the window.

The saving path and all the images under this path will display on the right side of
the window.



Click thebutton to add the current saving path to the favorites folder for the

next time of speedy opening.
Click thebutton to return to the upper directory.

Click following buttons to select picture view mode.

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Figure 2-83) Right click on an image or on the blank area of the interface to pop up theoperation menu, under which, [Rename], [Close], [Close All], [Delete All], [Delete],[Compare], [Save Compare], operations are able to performed.

Figure 2-9

There are two contrast mode: dynamic contrast and static contrast.


Dynamic contrast: The contrast between preview image and saved picture. In
preview status, right click picture bar will eject operation menu, then select[Contrast]. Dynamic preview picture will appear on the left side of the page, any
saved picture selected on picture bar will appear on the right side, and thepictures on the right side can be replaced at anytime. Clickon the right upper

corner to exit contrast.


Static contrast: Contrast between two saved pictures. Selecting an image, and by
right clicking picture bar, the operation menu is popped out; then selecting[Contrast], the selected picture will appear on the left side of the page. Clicking
another picture on the right side of picture bar, it will appear on the right side.
During static contrast, pictures on both sides can be replaced at anytime: firstly,
click the picture which needs to be replaced, and then click the picture to replace;the process will be completed.
To save contrast can save the pictures being contrasted. The contrast effect is asfollows.

Page 12Figure 2-10

Clickon the right upper corner to exit contrast.

⑥Shortcut Key
In order to make clients’ operation more convenient, the software provides shortcut
key functions. Specific functions are as follow:
Function
CaptureRecordvideosKey

Remarks

F10

Take pictures and automatically save the taken pictures.

F11

Press once to record, press again to stop recording
Not to display right thumbnails. A prompt box will

Close allF9

appear: whether to close all pictures, click [Yes] to takeeffect.

Save image
as

F8

Can change the picture format or picture saving
position.
Pause/ Resume the preview. When the preview is

Pause

F7

paused, a pause state prompt appears on the interface,
as shown in below

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3. Capture
3.1 Capture
Click the button to capture the image of the preview interface, support continuous
click;

Figure 3-1

3.2 Resolution
Resolution: select the resolution of previewing.

Figure 3-2

Note:


The High Speed binning resolution of Monochrome Cooling camera can increase
the frame rate;

The compress resolution of the HDMI series will increase the frame rate, but the CPU
usage is high;

3.3 Binning
Binning: Binning mode can improve the sensitivity of the image. The larger the valueis, the higher the sensitivity is.

Figure 3-3

Note: This function performs sum binning on the basis of the original data, the amountPage 14of data does not change, so the frame rate does not change;

3.4 Exposure Control
Set the exposure time of camera and then the value of real-time frame per Second(FPS) will show up.

Figure 3-4

Target Value: The adjustment of target value can change the automatic exposurebrightness of the image; the target value range for MIChrome series are 10~245; GT
series and HDMI series are 0-15.
Auto Exposure: Check the blank in front of the [Auto Exposure], the software
automatically adjusts the exposure time according to the brightness of the image toobtain an image with appropriate brightness. The software will provide different auto
exposure time range according to different models of camera. The specific value issubject to the actual interface.
Area Exposure: Check the [Area Exposure], the software automatically adjusts the
exposure time according to the image brightness in the area.
Manual Exposure: Uncheck the [Auto Exposure], system switches to [Manual
Exposure] mode, user can manually input the exposure time into the blank according
to his actual demands for the image, then click [OK] button to apply, or manually
adjust the exposure time linearly through the slider. The software will provide different
manual exposure time range according to different models of camera. The specificvalue is subject to the actual interface.
Gain: User can select the most suitable gear of gain according to his specificapplications and needs on images previewing. Greater gain brings the improvement

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of the brightness, but with the increasing noise.
Default: Click the [default] button to restore this module's parameters to the factory
default ones. The default setting of exposure is the [auto exposure].

3.5 White Balance
User can adjust the parameters of white balance according to the actual light source
to obtain better image effects which closely meet the needs of his.

Figure 3-5

White Balance: By adjusting the ratio of the three individual components of red,green and blue, the camera is able to reflect the true image color under various
illuminating conditions. The default setting of the white balance of the camera is
Auto-white balance. User can uncheck [Lock White Balance] under the environment
with stable illuminating conditions, while the user can check [Lock White Balance] tolock the current white balance setting under unstable illuminating conditions.
Area white balance: In the biology mode, when opening white balance options, and[Area White Balance] is selected, area white balance box will pop out on the preview
pictures. In the industrial mode, when opening white balance options, area whitebalance box will pop out on the preview pictures. The size of area white balance box isadjustable. Under stable lighting environment, move the area white balance box toany white part of the pictures, then adjust size, and check [Lock White Balance] tolock present white balance, which will make white balance calibration more accurate.
Gray: Check to converts a color image to a monochrome image.
Red, Green, and Blue(Gain): Manually adjust the gain values of the red, green, andblue channels for suitable white balance effect, adjusting range is 0~683

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Color temperature(CCT): The current close color temperature can be achieved byadjusting the three gains which are the above Red, Blue and Green. It can also be
manually adjusted and matched to the close color temperature according toilluminating environment. By the way of calibrating the white balance will be moreaccurately to achieve the correct color temperature. Color temperature setting range
is 2000K to 15000K.
Default: Click the [Default] button to restore this module's parameters to the factory
default ones. The default setting of white balance is [Auto-white balance].

3.6 Bit of Depth
Users can switch 8bit/16bit depth according to actual requirements.

Figure 3-6

Note:


The default bit depth of Monochrome Cooling camera is 16 bits;

The default bit depth of USB3.0 color camera is 8 bits;

The default bit depth of USB2.0 camera is 8 bits, and they can’t support 16 bits;

3.7 Image Adjust
Conduct real-time dynamic adjustment of pictures by changing parameter to achieve
ideal picture effect. Specific functions are as follow:

Page 17Figure 3-7

Hue: The shade of the color, adjusting ranges from 0 to 360.
Saturation: the purity of the color, the higher the purity, the more vivid performance,
the lower the purity, the bleaker of the effect, setting range is 0~255
Light: the brightness and darkness of the image, setting range is 0~255
Contrast: The higher the contrast is, the clearer the picture is, and the brighter thecolor is, setting range is 0~63.
Permeability: The enhancement of pictures, setting range is 0~48 for MIchromeseries and 0~63 for GT series.
Sharpness: to improve clarity of picture edges.
DPC: Reduce image bad points, range 0-3, 3 is default for FL-20.
Black Level: Adjust the gray value of dark background. The range is 0-255 and thedefault value is 12.
3D noise reduction: take the average adjacent frames of pictures, automatically filter
out the non-overlapping information, so as to obtain a purer and delicate picture. The
number of frames can be customized. The setting range is 0~5 for Fl-20, and default
is 3.
Flat Field Calibration: In actual microscope applications, the images captured by thecamera may be uneven or carry color patches, due to the problems of microscopes’

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light sources, optical path systems, or the dirty spots on the microscope eyepieces
and objectives. With flat field correction, those types of defects could be effectively
reduced, the imaging effects will be more uniform, and the color reproduction of theimages is smoother and more realistic.

Operations:
1) Click [Flat Field Calibration Wizard] to move the viewing field of camera to a blankbackground, as shown in the right figure (1);2) Click [Next] to move the first background to another new blank background, click[OK] to apply the Flat Field Calibration function, as shown in the right figure (2);Figure 3-8

Figure 3-93) Select [uncheck] to exit the flat field correction mode. If you need to apply it

again, re-check it, no need for repeating the wizard procedures again.
Note:1) The Flat Field Calibration requires the manually setting of the exposure time, sothat the image brightness will not overflow up or down, and all the pixel values arerange from 64DN to 254DN.
2) The brightness of other areas of the two backgrounds which are used forreferences of correction should be almost the same except that there are somemoving spots on the two backgrounds. These two backgrounds should not be
those of "significantly inconsistent".

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3) To standardize the background for the flat field correction, it is recommended touse plastic, ceramic or professional white balance paper as the standard samples.
4) Flat Field Correction requires that the background it use as reference to be ablank one and the light illumination on it is uniform. The correction should be
conduct again if the camera or the microscope changed their lens or objective.
Default: The settings will restore to default value when you click [Default]. Color tunedefault is 180, contrast default is 33, saturation default value is 64, permeability
default is 16, image enhancement save is defaulted to be unchecked, detailenhancement default is 1, and noise reduction default is 1.

3.8 Histogram
User can try the software function of color level Adjusting to obtain more realistic
images for observation and analysis.

Figure 3-10

The color level adjusting means to redefine the R\G\B color levels in each channel
and then re-allocate of the pixel values in among. Adjust the color level (gradation) toincrease the range of the highlight area in the image, image becomes brighter, or
using the contrary way to make the bright image darker. User can also adjust thecomponents of the RGB three channels separately to change the image color of thecorresponding channel.
Manual Color Level: User can manually adjust the image's dark tone (left gradation),

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the gamma and highlight brightness level (right color gradation) on the histogram toregulate the image's tones, such as the contrast, shading and image layers, thenobtain the correct balance of the whole image.
Auto Color Level: Check [Auto Min] and [Auto Max] to automatically adjust thebrightest and darkest pixels in each channel as white and black, and then re-allocate
the pixel values in proportion.
Gamma: adjust the median of the color level, setting range is 0.64 to 2.55;
Histogram display: Histogram supports linear or Logarithm mode.
Default: Click [Default] button to restore the module's parameters to the factory
default ones. The default of color level adjusting is manual, and the default gamma
value is 2.10.
Note:1) The composing and displaying of the histogram curve is the result of thesoftware’s running real-time data statistics, so some the sources of the software
will be occupied. When this module applied, the camera frame rate may beslightly affected to drop. When the module stops working, the data statistics areturned off and the frame rate of the camera is able to reach to the maximum.
2) After canceling the automatic color level adjusting, the level value will be thedefault (0 and max).

3.9 Temperature Control (only for Cooling camera)
The software supports the temperature adjustment of cooling camera; noise reduction
can be achieved by reducing working temperature of picture sensor.

Figure 3-11

Page 21Current: to display the present temperature of camera.
Cooling: three options – normal temperature, 0°, low temperature – are set; selection
can be made based on actual request.
Fan Speed: Control the fan speed. There are three options on Monochrome Coolingcamera: high, medium and low, and the default is High. There are 6 options on Color
Cooling camera, and the default is 3. Low speeds can reduce noise, but the heatdissipation is slightly less effective.
Default: restore present parameter to factory default status. “Low temperature”
refrigeration is default.
High temperature warning (FL 20BW): When the ambient temperature is too high,the software will pop up the temperature warning message in time, and the indicatorlight of the camera will turn to red flashing.

Figure 3-12

3.10 File Save
Capture the currently needed data from the real-time video data stream and record it
into image format for later development and analysis.

Figure 3-13

Clickthe button to capture a live previewing image

Pop-up Windows Saving: the saving window will be popped up after the button

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Captureisclicked,itsupports

five

formatsforsavingpictures:

JPG\TIF\PNG\DICOM\MPTIF. MPTIFF is only supported on Dhyana 400DC. DICOM
and MPTIF are not supported on Mac system.
File name: The name of the file to be saved is named as "TS” format by default, and it
allows to name the file personally by user. The file name cannot contain special
characters \/:*?"<>|, special characters cannot be entered, and an error message will
pop up, as shown in below.

Figure 3-14

The software supports the format of "custom + time-stamp" for naming. There are fiveformats of time-stamp naming.

Figure 3-15

Format: The image formats could be saved as JPG\TIF\PNG\DICOM. The default
format is TIF. These four image formats can be checked separately or simultaneously.
The captured images in different formats will be displayed at together when theirsaving formats are selected to be simultaneously.
1) JPG: it is an information-losing and compressed image saving format, its image
size is small, but the final quality of the image is degraded compared with theoriginal captured image because of the image data- losing during being saved.
2) TIF: A Lossless image saving format, which can save all the data transmitted fromthe camera to your saving device without losing, this format is good for the saving
and converting of images with high image quality requirements.
3) PNG: Portable network graphics is a lossless but compressed bit-image format
that uses the way of lossless data compressing algorithm derived from LZ77 witha high compressing ratio and a small file size.
4) DICOM: Digital imaging and communication of Medical, an international standard
format for medical images and related information. It defines a medical image

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format that can be used for data exchanging and meet the requiring of clinicalpractices and applications.
Path: To save the image files to the folder that is under the certain of defined path.
User can click the [Browse] button to change the saving paths. The default saving
path is C:/Users/Administrator/Desktop/Image. The path cannot contain special
characters \/:*?"<>|, special characters cannot be entered, and an error message will
pop up.
Saved with time format: The capture time will be displayed in the lower right cornerof the picture.
3D Denoise Save: Obtain low-noise images by acquiring and comparing image dataof several consecutive frames, and then filtering out non-overlapping information. The
number of frames can be customized. The range is 1~99, the default is 10.
Note:

To get 3D denoise image, so it will take longer time than taking a general image.



3D Denoise Save is only suitable for capturing static scenes;

3D noise reduction has no effect on video recording.

3.11 ROIROI (Region of interest) is an application that user runs the software to define awindow area of interest within the effective and sensitive detecting area of the camera
sensor and just allow the image information within this defined window to be read out
and then show out a localized area of image. A smaller ROI area reduces the amountof information and tasks of image transferring and computer processing, which results
in increased frame rate of the camera.

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Figure 3-16

Select ROI: Using a computer mouse. Check on the ROI frame, then move thecomputer mouse to the previewing image to define the window area you would like it
to be your ROI, this window area will display the coordinate value and resolution of thecurrent running point. Click on the [√] below the cursor to apply the ROI settings into
effect.
Set Area and Coordinate of ROI: Manually entering the starting point coordinate
value and the resolution size to define the exact ROI area. Enter the actual point offset
position of the rectangular area as well as the width and height, then click [OK] toapply the ROI settings into effect.

3.12 Mask

Figure 3-17

Select Mask: Using a computer mouse. move the computer mouse to the previewing
image to define the window area you would like it to be cover, this window area will
display the coordinate value and resolution of the current running point. Click on the [√]below the cursor to apply the cover settings into effect.
Set Area and Coordinate of Mask: Manually entering the starting point coordinate
value and the resolution size to define the exact cover area. Enter the actual point
offset position of the rectangular area as well as the width and height, then click [OK]to apply the cover settings into effect.
Transparency: Manually select the brightness of the unselected area

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3.13 Imaging Stitching(Live)The model of real-time image stitching supports to acquire several individual images
with adjoining positions on the specimen or sample, and to have them combined into
astitched image which present the whole form of the specimen.

Figure 3-18

Stitching Speed: Two options: High Speed and High Quality. the default is High
Speed.
Background Color: The default background color of the invalid area on thestitched-to-composed image is black, but you can change it. Click on

to select thedesired color in the palette. And it will effective after stitching is completed.
Operating Procedures
1) Clicking [Start Stitching], software will pop up below operating prompt;

Figure 3-19

2) Move the sample to other areas through the stage, and move no more than 3/4.
The navigation display frame in the stitching window is green, and the software
will perform one stitching; each time the sample is moved, the software will
perform one stitching;

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Figure 3-20

Figure 3-21

3) If the navigation display frame is red, it means that the stitching area is exceeded
and stitching cannot be performed, and the sample needs to be moved back to
the location near the last stitching area.

Figure 3-22

4) Click [Stop Stitching] to end the stitching, and a stitched and composited image
will be generated in the image bar.
Note:1) It is recommended to conduct a white balance correction and flat field correction
before starting the stitching to ensure to achieve the best quality images.

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2) Since the stitched images are in huge sizes and will surly occupy massive
memory of the computer, the stitching process will occupy computer memory,when the processing of stitching taking up 75% of the computer memory volume,the stitching module will stop working automatically. So it is recommended to
conduct the stitching operations through a computer with sufficient memory
volume.
3) 32-bit computer operating system does not support dynamic stitching images.

3.14 EDF(Live)EDF makes it possible to stack and merge the images acquired by microscope withdifferent focused panels or positions on a certain thick specimen or sample. The new
stacked and merged image is able to present the clear full forms and information of
the specimen or sample.

Figure 3-23

Quality: The higher quality level of the new image requires, the slower speed in its
merging, but results in coming out of image with better quality.
Operating Procedures
1) Click the [ Start EDF] to start the depth of field extension operation;2) Continuously turn the fine knob of the microscope to focus on one plane to next
other, the software is able to merge the obtained plane images instantly and showout a live and clearest image.
3) Click the [Stop EDF] to end the stacking and merging application, a new merged
image including all the depth focusing information will be generated in the image
gallery.

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3.15 Realtime DyeThis mode is generally used when taking fluorograms with monochrome camera tocolor monochromatic fluorescent image, and take a photo to obtain a dyed image forlater synthesizing a complete fluorescent image.

Figure 3-24

Current: This window displays the currently available colors.
Clickto make the full color palette appear, from which, user has many morechoices of color to pick.
Add to New Dyes: To add selected colors on the palette into the new dyes.
Dye Type: Display seven commonly colors.
New Dye: Displays user-defined dye colors.
Delete: Delete the selected user-defined dye color;
Apply: Apply the selected dye color to the image;
Cancel: To cancel a certain kind of dyes added through custom mode.

3.16 Video Record
Click on [Video Record], Save the image data into video formats for playing back toobserve the process of the samples’ live movements.
Encoder: The software provides two compressing formats: [Full frame (NoPage 29

compression)] and [MPEG-4], the video sizes generated by different compressing
formats are inconsistent, and the user may choose either one format according to his
actual needs.
Total Frame: Capture images according to how many frames was set to be captured,setting range is 1~9999 frames.
Total Time(s): The length of time of capturing images based on how long the time
was set to, setting range is 1~9999 seconds.

Figure 3-25

Delay time: There will be a delay in capturing images based on the setting period andusers can choose three-time units: minute, second and millisecond. The range is 1
millisecond to 120 minutes.
Playback Rate: Record according to the set playback frame rate
Video Format: AVI\MP4\WMA are supported, the default is AVI format;
Save to Hard Disk: Instant loading and writing in. The image data is being loaded
and written into the hard drive of the computer during the image capturing process. In
this mode, the transmission speed from the camera to computer will reduce due to thecomputer’s loading and writing in images data into its hard drive, so this mode is notsuitable for capturing the images of quickly changing scenes or backgrounds, but it issuitable for long-time period of capturing.
Save to RAM: The image data is saved in the RAM of the computer temporarily, after

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the image capturing, all the image data is transferred to the hard drive of the computer
to save. Select Save to RAM and enable the RAM to save images. The maximum
numbers of images that can be saved by RAM and display will be shown out. Thissaving mode allows the camera to maintain a high transmission speed of the images
when capturing and avoid data losing due to a low transmission speed. This saving
mode is limited by the volume of the computer's RAM, so it is not suitable for long-time
period or huge volume of capturing.
Default: Click [Default] button to restore the module's parameters to the factory
default ones. The default saving mode is the compressed mode with full resolution
frame, with the total number of frames to be 10 frames, and the total default capturing
time is 10 seconds, image data default to be saved to the local hard drive.

3.17 Trigger (FL 20BW)
There are two modes of image output: frame mode and stream mode.

Figure 3-26

Frame mode: external trigger mode, which takes frame as the unit to output image
through trigger, and can be divided into hardware trigger mode and software trigger
mode.
Stream mode: real-time display mode, which embeds image data in the stream with

Page 31

the output mode of data stream. Images are looped out like water.
Off: Indicates that the hardware trigger mode is off at this time, and the camera
always takes images in stream mode.
On: The camera is switching to the trigger waiting mode. Only when the trigger signal
is received, the images will be taken.
Enable hardware trigger to enter standard trigger mode. The standard trigger mode
contains the following configuration modules.
1) Exposure
Timed: Exposure time is set by the software.
Width: Exposure time is set by the width of the level.
2) EdgeRising: The trigger signal rising edge is effective.
Falling: The trigger signal falling edge is effective3) Exposure Delay
Users can set a delay time of how long it takes for the camera to start exposure when
the signal is received.
Software trigger mode
Click the snap button, the command will be sent to the camera to take images, only
one image output at a time.
Notes:
1) When switching between the external trigger mode and the real-time preview
mode, it will take effect directly after checking different modes.
2) When the trigger mode, exposure mode, edge and delay settings are selected,
they will take effect directly.
3) Save parameters is supported. The next time you open the software, it will still bein the state before closing.
4) External trigger mode can control the beginning and end of images taking. The
operation steps are as follows: select the trigger mode first, then configure thePage 32

parameters (such as image format and image number), then click [Capture frame],and finally input the pulse signal.
5) Close the resolution, bit depth, ROI and video recording functions after entering
the external trigger.
6) Minimum trigger cycle: readout time + exposure time. Such as FL 20BW readout
time =1000/ frame rate and the exposure is 50ms, then the trigger signal highlevel + low level ≥ readout time + exposure time = 125ms+50ms.

3.18 Image Process (FL 20BW)
Frame Integral: Continuous multiple frames are captured according to the preset
conditions, and multiple frames are accumulated into an image to preview the integral
effect in real time.

Figure 3-27

1) Frame integral: Taking the continuous preset frames and accumulate them into
one image.
2) Time integral: Taking all frame data within the preset time and accumulate theminto one image. The frame number is the integral time divided by the exposuretime.
3) Preview: According to the preset parameters, the integrated effect will be
displayed in real time on the screen, and the best result will be adjusted before
drawing.

Page 33

Notes:1) An appropriate integrated frame number should be set for integral imaging,otherwise the image brightness will be too high and distortion will occur.
2) Frame integral and time integral cannot be used at the same time, and only one
mode can be selected at a time.

Dark Field Correction:
1) The default correction box cannot be checked. It will be valid after importing andsetting the coefficient. Automatic check means turn on dark field correction. At thistime, the check state can be removed, which means turn off dark field correction.
2) Click the correction button and operate according to the pop-up prompt. Click next
to calculate the correction coefficient automatically.
3) The frame number is 10 by default (users can set different frames according totheir needs, and the range supports 1~99).
4) Import and export are the import and export correction coefficients respectively.
5) Dark field correction should be made for different exposure time and scenes.
6) Turn off the software and parameter groups can save the number of frames, but
clear the correction box and coefficient. Turn on the software next time need to do
the correction again.

3.19 Delay Capture

Page 34

Figure 3-28

Total Time: Capture images according to set time, default is 1, the setting range is1-9999s.
Total Frame: Capture images according to how many frames was set to, systemdefault to be 10 frames, setting range is 1~9999 frames
Playback Rate: Record according to the set playback frame rate
Interval Time: Interval time for capturing every image, three time units options:
minutes, seconds and milliseconds, the default is 0 minutes, and the range is
0ms-120min.
Delay time: There will be a delay in capturing images based on the setting period;
three time units options: minutes, seconds and milliseconds, the default is 0 minutes,
and the range is 0ms-120min.
Video Format: AVI\MP4\WAM are supported, the default format is AVI;
Capture Frame: Capture and save multiple frames of images continuously according
to the preset parameters, click [Stop] to terminate the continuously capturing before
the capturing task is finished.;
Capture as Video: Capture multiple frames of images according to presetparameters and convert them into a movie directly. Click [Stop] to terminate thecontinuously capturing before the capturing task is finished.
MPTIF: When Dhyana 400DC is connected, it can support save images as MPTIF
format. If both TIF and MPTIF formats are selected, only MPTIF format images will be
generated.
Note:
There is a quantitative relationship between the total time, the total number of frames,
and the playback frame rate. The value of total time = total number of frames/playback
frame rate is rounded down.

Page 35

3.20 Save Settings
This software supplies the function of managing the groups of experiment parameters
for the users’ conveniences using the camera in different applications or platforms.
User can save and re-load the set parameters from different experiment statuses andthen apply them to the new experiments projects, this function will surely save theusers’ time, develop the working efficiency and ensure the reprehensibility of theexperiment results. The parameter group saver allows the users to save all thesoftware functions and modules mentioned above (exception is the flat field correction
module).

Figure 3-29

Copying and loading the files of parameter group to other computer platforms canrealize the cross-platform applications of these parameters, so the user's
experimental conditions are ensured to be as uniform as possible;
Group Name: To name the parameter group, input the name you desired and load it,
the window's context menu will display those parameter groups which have beensaved or loaded.
Save: To save the current parameters into a named parameter group file. up to 50
sets of parameters can be saved.
Load: Load the parameter group to current working status.
Delete: To delete the currently selected files of parameter group.
Export: Save the files of the parameter groups to specified folder documents; Reset
all: Restore to the factory default settings will delete all the files of parameter groups
set and saved by the users before.
Import: To load the selected files of parameter group from the selected folder.

Page 36

Reset All: Clear all parameters set by the user and restore to default settings of thesoftware.
Load Fluorescence Mode: After clicking this button, the exposure mode is switchedto manual exposure, the exposure time is 100ms, and the automatic level is
automatically checked.

3.21 Light Frequency
Users can select the corresponding light source frequency according to the actual
applications or conditions. Suitable frequency of light source is good for the normalityof the live images and excludes the phenomenon of stroboscopic on the live images.
The default light source frequency is the frequency of direct current.

Figure 3-30

3.22 Other Settings

Figure 3-31

Negative: Change the color of the current image to its inverse color.
HDR: Click to achieve higher dynamic range and more image details for better image
quality.

3.23 Auto Focus (only for Auto Focus camera)
Continuous Focusing: Select the area to be focused in the preview screen; then, thecamera will conduct continuous focusing on selected area until it’s completely clear.
When the focal length is changed due to movement of sample or camera, camera will

Page 37

automatically fix the focal length to ensure the clear effect of pictures.

Figure 3-32

One-Shot AF: Select the area to be focused in the preview screen; camera willconduct one-time focusing on selected area. After focusing is completed, the focal
length will stay unchanged when sample or camera moves, but it is required to selectthe area needs to be focused.
Focusing Location: Focusing location can be manually adjusted; the focal length ofcamera will change according to location change.
C-Mount: Automatically move to the C interface position

Page 38

4. Image

Figure 4-1

Click

to save picture as any format of JPG\TIF\PNG\DICOM; saving window will

be popped out as shown below.

Figure 4-2

Clickscreenshot button on the right upper corner of the preview window to crop

picture, to select the interested area in preview image with mouse, then double left
click or double right click the mouse to complete screenshot. The screenshot will

Page 39

appear on right picture bar, click

to save current screenshot. If there is no need tosave the screenshot, right lick to exit crop window.

4.1 Image Adjust
Adjust the image parameters to revise the effects of the captured images.

Figure 4-3

Brightness: The brightness of the image, the default value is 0, adjusting range is-255~255.
Gamma: Adjust the brightness of the image displayed on the monitor; the default
value is 1.00, adjusting range is 0.01~2.00.
Contrast: The ratio between the darkest areas and brightest areas of the image, thedefault value is 0, adjusting range is -80~80.
Saturation: The purity of the color, the higher value of the saturation, the brighter thecolor is, the default value is 0, adjusting range is -180~180.
Sharpen: Focus on blurred edges of the image to improve the sharpness or focal
length of a certain part of the image, leads to a more vivid color in the specific area of
the image. The default value is 0, and adjusting range is 0~3.
Finishing the adjustments of the parameters for the image, click [Apply As A New
Image], the set parameters will be applied to the original image which then change toa new image with the adjusted characters. The new image also be saved by thiscommand.

Page 40

Default: Click the [default] button to restore the adjusted parameters to the factory
default ones.

4.2 Image Dye
Need to color blank-and-white pictures firstly. Based on actual request, users canselect color through color editor and also to select corresponding fluorescent dyes.
After color selection, click [Apply as A New Image] to apply color on picture, to gain arealistic color picture almost coincident to that observed under a microscope. Click[Cancel] to cancel dyeing.

Figure 4-4

Current: to show present selected dye type. Software provides seven colors usuallyused, click

to get the integrate palette to select more abundant colors as shown

below.

Page 41

Figure 4-5

Dye Type: To provide abundant colors for use; set the preferable color through rightcolor parameter setting, click and add it to customized color to facilitate future use.
Add to New Dye: selected color on palette can be added to new dye.
Cancel: Cancel a certain kind of customized added dyes.

4.3 FluorescenceIn the researching fields of biological related sciences, different fluorochromes areused to label different cell structures. On the applications of micro-fluorescentobservations, a complete fluorescent cell image contains multiple of fluorescent areas
which belong to different cell structure who has its own special affinity to certain offluorochromes.
Due to the limitation to the wavelength of the emission fluorescence in the microscopeturret, user can only observe one color of image each time. Fluorescent combing

Page 42Figure 4-6

Operating Procedures:
1) Select the image which needs fluorescent channels combing from its path andopen onto the window,
2) Click on [Start Color Composite] to select a group of images with the sameobservation field as the one opened before. The maximum pieces of images could
be selected to combine together are 4 pieces. The operating directions window
will pop-up, as shown below.

Figure 4-73) Select the original picture needs fluorescent synthesis on right picture bar; thesynthesized picture will be displayed on preview windows, as shown below.

Page 43

Figure 4-84) Click [Apply as A New Image] to add the combined image to the image gallery
and let it display in the center of the working interface, the fluorescent combing
processing finished.
Offset: The mechanical vibrations from the fluorescence filters’ switching can lead tothe pixel drifting of each captured single-channel image to others when they were
captured individually, so it leads to the situation that these captured single-channel
images are not exact under the same observation field and area, with slight drifts. Drift
Correction module is able to let the user to correct these pixel drifting and finallyensure all the single-channel images to be under the exactly same observation field
and area, thus results in the perfect results of multi-channels combing. One correction
unit stands for one pixel, click on [0,0] to restore to the original point.

4.4 Advanced Computational Imaging
Mosaic software offers users with three kinds of Post-processing technologies for
Image packages. They work on the merging of batch of captured images.

Page 44

Figure 4-9

Extend Depth of Field (EDF): Pick up those images which were captured from thesame observation field but not on the same focused planes, use those clear focusedareas of each captured image together to merge a new image which carries all clear
focused areas in whole image.
Image Stitching: Stitch several images which have overlapping parts to one andother into a large, seamless, high-resolution image.
High-Dynamic Range(HDR): Take a batch of images with different exposure times,
merge the three images which are the insufficient exposure one, the moderate
exposure one and the excessive exposure one into new image with high dynamic
range.

Operating Procedures:
1) User may select the image processing method he needs. Pick up multiple ofimages with the wizard of operation. Let us take the EDF processing as an
example.

Figure 4-10

2) Then click on the[Combination]at the bottom of the interface;3) The combining process requires for a while, the window will display theon-progress, for example: EDF 4/39.
4) When the combing is completed, a thumbnail of the combined image will be

Page 45generated and present in the left menu bar as below.

Figure 4-11

5) Click on [Apply As A New Image] button, The new combined image will be add tothe image gallery and shown up in the center of the working interface and the
combing process comes to ending.

4.5 Binaryzation
Mosaic software provides user with image binaryzation, which is to set the grayscalevalue of the pixels on the image from 0 to 255, it is the process of presenting thewhole image with a distinct black and white effect (A binary image, which is a digital
image that has only two possible values for each pixel).

Figure 4-12

Page 46

4.6 Histogram
When handling with pictures, more realistic colorful pictures are usually needed to use
for observation and analysis. Color scale adjustment can help users to obtain better
picture effect, as shown in the following picture.

.
Figure 4-13

Color Scale Adjustment: redefine R/G/B in each path, and then proportionallyredistribute the pixel value among them. The adjustment of picture’s color scale canenlarge highlight area of the picture and brighten picture, as well as to darken thebrighter picture; RGB three paths can be adjusted separately to change the color of
picture in corresponding path.
Manual Color Scale: Users, with histogram, can manually adjust the dark shade (leftcolor scale), gamma and highlight brightness level (right color scale) to calibrate
picture shade tone, including contrast, shade and image hierarchy, and to balance thecolor of the picture.
Automatic Color Scale: Check Automatic, customize the brightest and darkest pixelin each path as white and black, and then proportionally redistribute the pixel values
between them.
Apply: Apply current parameter setting in picture and generate a new picture. The
new picture can be saved as separately.
Cancel: Click[Cancel] button to cancel parameter of the module.

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4.7 Smooth
Software provides users with three image smoothing techniques for blurring images toreduce the noise and the level of detail: Gaussian Blur, Box Filter, Median Blur, setting
range is 0~30.

Figure 4-14

Default: Click the [default] button to restore the module's parameters to the factory
default ones.
Radius: the bigger the radius is, the stronger the image processing ability is and thesmoother the image is.
Apply: When the parameter adjustments are completed, click [Apply] to make theparameter settings work into effects on the original image and generate a new image,
the new image can be save as to other path or documents.
Cancel: Click the [Cancel] button to cancel the parameters set into the module before.

4.8 Filter/Extract/Inverse Color

Figure 4-15

Color: Select Red/Green/Blue.
Filter Color: check the color level information in each channel and combine thePage 48

complementary colors of the mixed colors with the base color. The combined color isalways the brightest color.
Extract Color: Extract the certain of color from the RGB color group.
Inverse Color: Invert the colors in the RGB group to their complementary colors.
Apply: When the parameter adjustments are completed, click [Apply] to make theparameter settings work into effects on the original image and generate a new image,
the new image can be save as to other path or documents.
Cancel: Click the [Cancel] button to cancel the parameters set into the module
before.

4.9 Deconvolution

Figure 4-16

Iterations: Number of iterations;
Kernel Size: Deconvolution kernel window size;
Note: This function is not supported on Mac system.

4.10 Automatic Counting
Start Counting: Click it to enter the counting process;Figure 4-17

Page 49

Figure 4-18

All: The counting area is the full image;
Rectangle: Left-click to select two endpoints to draw a rectangular image, and thecounting area is the selected rectangular;
Polygon: Left-click multiple times to draw a polygon, double-click to end the drawing,
and the count area is the selected polygon;
Restart Counting: Back to the start counting interface;
Next: Go to next step;

Figure 4-19

Auto Bright: Use algorithms to automatically segment bright objects from the dark
background;
Auto Dark: Use algorithms to automatically segment dark objects from brightbackgrounds;

Page 50

Manual: Manual segmentation is based on the histogram distribution of the image,which can be adjusted by the two vertical lines on the left and right in the histogram, or
can be adjusted directly by inputting values in the upper and lower limit edit boxes of
the histogram;
Dilate: Change the size of the cells in the image to expand the borders of bright cells
and shrink the borders of dark cells;
Erode: Change the size of the cells in the image to expand the borders of dark cells
and shrink the borders of bright cells;
Open: Change the shape of image cells. Assuming that the image is a bright cell on adark background, clicking this item will smooth the cell boundary, separate connected
cells, and remove small black holes in the cell;
Close: Change the shape of image cells. Assuming that the image is a bright cell on adark background, clicking this item will fill in the gap of the cell, and at the same time
stretch and highlight the cell that is close to it;Fill holes: Fill holes in image cells
Restart Counting: Back to the start counting interface;
Back: Back to the previous operation process;
Next: Go to next step;

Figure 4-20

Contour: Use contour lines to represent the divided cells;
Area: Use padding to represent divided cells;
Auto Cut: draw the boundary directly according to the actual contour of the divided
cell;
Manual: Manually select multiple points on the interface, and divide cells according toPage 51

the selected points;
No Cut: Do not divide the cell;
Merge: Merge separate cells into one cell;
Bound Process: When calculating the number of cells, cells with incompleteboundaries in the image will not be counted;
Restart Counting: Back to the start counting interface;
Back: Back to the previous operation process;
Next: Go to next step;

Figure 4-21

Add: Add the type of calculation to the statistical result;

Page 52

Delete: Delete type of calculation;
Minimum: Set the minimum value for dividing cells, that is, cells smaller than theminimum value will not be counted;
Maximum: Set the maximum value for dividing cells, that is, cells greater than the
maximum value will not be counted;
OK: Start counting cell data;
Draw Target: Check this option after the automatic counting is completed, and you
can manually draw the missed cells on the interface;
Export Report: Export statistical cell data to Excel file;
Restart Counting: Back to the start counting interface;
Back: Back to the previous operation process;4.11 Automatic Counting Property

Figure 4-22

Font: Set font and size, default is Arial, 9, click

to bring up the font board to selectthe desired font;
Font Color: Set font color, default is green, click

to bring up the color palette to

select the desired color;
Target Color: Set cell display target color, the default is blue, select it and clicktobring up the color palette to select the desired color;
Contour Width: Adjust the cell display outline width, default is 1, range 1~5;
Precision: Set the number of decimal places for the maximum and minimum values.

Page 53

By default, 3 decimal places are reserved. The range is 0~6.

Page 54

5. Measure
Mosaic provides tools for images measuring. For example, if you want to know the
distance between two points in the image, using the measurement tools, you cansimply draw a line between the two points and get real-time measurement data.

Figure 5-1

Mosaic provides users with a rich set of measurements in image analyzing. The
principle of the measuring function in Capture is based on image pixels as the basic
execution unit. For example, the length of the line feature is determined by the numberof pixels along the line. Pixel-level measurements can be converted to more practicalunits, such as millimeters(mm) or inches, using measurement calibration module inthe software and calibration ruler.

Page 55

5.1 Measure Tool

Figure 5-2

Straight Line: Draw a line segment graphic and then finish drawing with one more
click, arrows at endpoints. Press [ctrl] to draw horizontal or vertical lines.
H Shape Straight Line: Draw a line segment graphic and then finish drawing with
one more click, vertical lines at endpoints.
Three Dots Line Segment: draw graphic with three dots line segment, finish drawing
when click for the third time.
Multiple Dots Line Segment: draw graphic with multiple dots at the same direction,
single click to draw and double click to end drawing.
Parallel Line: Draw a line segment, then left click again to draw its parallel lines,
double- left-click to finish drawing.
Vertical Line: Draw a line segment, then left click again, draw its vertical line, doubleleft-click to finish drawing.
Polyline: Left click, add a new line segment to the existing polyline, double- left-click
to finish drawing.
Rectangle: Left click to select two endpoints to draw a rectangular, the length, width,
perimeter and area of the rectangular can be measured,
Polygon: Left click to draw the polygon multiple times, double click to finish drawing.
Ellipse: Left-click to select two endpoints to draw an elliptical graph the perimeter,

Page 56

area, major axis, short axis, and eccentricity of the ellipse can be measured.
Roundness: Select the first and second segment passing through the center of thecircle on the contour of the measured circle. After drawing, the value of the degree towhich the cross section is close to the theoretical circle is obtained. The value is equal
to two. The difference of the secondary line segment is divided by two.
Radius Circle: Left-click to select the center of the circle, and click again to define theradius length, then click to finish drawing.
Diameter Circle: Click two times to define the two diameter endpoints separately todraw the circle, then one more click to finish drawing.
3Point Circle: Click three times separately on the selected circle, and then one more
click to finish drawing.
Concentric Circles: Draw a circle with its radius, Click the position to be the center ofthe circle, then double click finish drawing.
4Point Double Circle: First click to position the center of the first circle, second click todefine the radius length of the first circle. The third click to position the center of thesecond circle, the fourth click to define the radius length of the second circle.
6Point Double Circle: Click three times to select three points on the first circle, andclick another three times to select the three points of the second circle, then end thedrawing.
Arc: Draw a curved line by taking three points on the circle, finish the drawing with thethird click.
3Point Angle: the angle between two connected lines. Click to draw the endpoint andintersection point of the two lines, take the third click to finish drawing.
4Point Angle: the angle between two unconnected lines. Click to draw the endpointsof the two lines, position the intersection point and take the fourth click to finish
drawing.
Dot: Dot marking or dot counting, manually count the quantity of targets or mark them.
And record the XY coordinate value of the point.
Paintbrush: Draw any curve on the preview screen.
Arrow: The arrow mark is made by two times of clicks.

Page 57

Text: Add a text note to the image.
Select: The measured graphics or text notes on the image are able to be selected.
When the mouse moved to the vicinity of the graphics or texts, the mouse symbolchangesto, you can drag or use the [Delete] button on the keyboard to delete

the selected graphics and text.
Delete: To delete the drawn or measured graphs.
Back Undelete: Undo the last delete operation.
Clear All: Delete all the drawn and measured graphics or texts on the current layers.
Combined: When saving the image, the drawn or measured graphics or texts will be
attached onto the image. The default status of the software to this module is active.
Data Property: Each graphic has corresponding data type, such as length, angle,
slope, diameter, perimeter, area and etc. Draw graphics after checking and theinformation will appear on pictures at the same time.
Right click the mouse to quit drawing state and switch to selecting state. Click the rightmouse button again to restore the drawing state.
When the mouse is in thestate, scroll the wheel to zoom in on the picture,and hold down the mouse to drag the picture.
When the mouse is in thestate, scroll the wheel to zoom in on the image, holddown the mouse on the graph to drag the graph.
All drawing and measuring graphic data information will be saved in the measurement
table. Click [Export to Excel] or [Export to TXT] to transfer the data information to
EXCEL form format or TXT document format. Click [Copy] to copy the entire table toother places for paste.

Figure 5-3

Page 58

5.2 Calibration
The calibration ruler converts the number of pixels into meaningful measurement units,
and supports conversion units of nm, um, mm, inch, 1/10inch, 1/100inch, and1/1000inch.

Figure 5-4

Operating Procedures:
1) Click [Draw], and draw a straight line on the image, press [ctrl] to draw horizontal
or vertical lines;
2) Enter the length and unit represented by the number of pixels of the straight line;3) Enter a name for the calibration scale;4) Click [Apply] to save and use the calibration ruler;

View/Edit Calibration Table: Users can create multiple groups of calibration scales
at the same time for the switching uses of multiple measuring application scenes. The
calibration scales can be viewed or edited on the calibration table.

Figure 5-5Open the calibration table, check the box under “Current” bar and select calibration
ruler. The software defaulted calibration ruler should be applied to all measureprojects. Selecting calibration ruler, calibration ruler can be edited and added on theright side, and mouse right key can eject menu bar as well: [Cancel] can cancel
selected calibration ruler, but the calibration ruler being applied cannot be canceled;[Load] can guide in previously saved calibration table; [Save As] can derive thePage 59

whole calibration table and save it.
Resolution is the preview resolution of the new calibration ruler. Switching theresolution, calibration ruler and measurement data will be automatically convertedwith resolution.
Note: Calibration processing can be performed more accurately with a micrometer.
Using an incorrect calibration table will lead the measurement results to be completely
wrong. Special cautions must be used to select the correct calibration table before
working on images measuring.

5.3 Measure Layer
Measure Layer: Creating multiple layers on the image, user can make different
measurements on different layers of the image.
This layer creating module meet the needs of a large number of image measuring andimage further processing applications.

Figure 5-6

If some targets on the current image have already been measured, the layer creating
function will automatically create a [Background] and named it as [Layer 01], thecurrent measurement results will be automatically displayed in [Layer 01].
You can choose to display the measurement data from different layers or choose toview the different layers.
Check the box which the related layer belongs to under the defined window of[Current] to display the layer you want to preview. Check on different box to switch toPage 60different layer you want to display.
Check on the box under the defined window of [Visible] to display the measurement
results of the corresponding layer.
Click [New] to create a new layer, you can customize the new layer name. The default
layer naming principle is by [Layer 01], [Layer 02], [Layer 03], it means that the layer
name with increased number, which related from early to later by the time the layer
was created.
Click [Delete] to delete the selected layer.
Click [Rename] to rename the selected layer.

5.4 Graphics Properties
Define the property of all measure tools. Specific information are as follows:

Figure 5-7

Global: Create or change a name on the blank right next to the [Name] column;
Show Name: Check if you do not need to display of the name;
Precision: choose the display precision after the decimal point, the default value is 3,
the range is 0~6.

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Line Width: To display line width of the measurement tool currently applied on theimage. The default is value 1, the range is 1~5.
Line Style: Select the styles of measurement tools currently applied on the image.
The default style is the solid line, software supporting solid lines, dashed lines, dottedlines, dotted lines, and two dotted lines.
Graphics Color: choose the colors for the measurement tools applied on the image,the default color is red, colors could be chosen from […].
Font: Choose the font format for the currently applied measurement data, the default
format is [Arial 20], click on […] to choose the desired format.
Font color: Choose the color for the currently applied measurement data, the default
color is blue, click on […] to select the desired color.
No Background: check or uncheck the blank of True to set the background astransparent background, or with background, transparent background is the default
setting.
Background Color: Select the background color for the current measurement dataon the image. Click out […] to choose the desired background color, the default
background color is white.
Apply to All: Apply all the set parameters above to the measured graphs.
Default: Resort and apply the default parameters settings to the measured graphs.

5.5 Metrics Flow
Mosaic provides powerful metrics flow function for convenient and semi-automatic
measurements in the passing-rate detection of batches of industrial devices or parts.

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Figure 5-81) Open a group of the devices or parts images saved in the image gallery.
2) Select the image of the standardized sample (or we call this image as thereference image) to calibrate and set the tolerances for later measurements anddetection;3) Click [Start Building a Metrics Flow] to create a new metrics template;4) Use the different measurement tools to measure or draw any of the desired
shapes on the reference image opened before. The running template will record
the whole measuring or drawing process and save the measuring results or
drawing graphics as the reference specifications.

Figure 5-95) After setting the referencing information on the template, user can name thePage 63

template personally, click [Save] to complete the setting and saving of themeasurement flow template.
6) Click [Start Applying A Metrics Flow], select the created template, click [Run]button to apply the template, click [Delete] to delete the template created andsaved before.
7) Select the image to be detected and operate as the procedure as that on creating
template.

Figure 5-108) After the operating of the software on the image to be detected, [Run] button will
be released and a window showing the detecting results will pop up automatically.

Figure 5-11

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Figure 5-129) Click [Export to PDF / Excel], to save as PDF format or export to in Excel format
with the detecting results.
10) Continue to click [Run] to select the next image need to be detected, repeat theprocedures of steps 7, 8, and 9 directed above;11) After finishing all the images need to be detected, click [Stop Applying a Metrics
Flow] to stop the working of the software function of measurement flow.

5.6 Manual Class Counting
User can classify and count different categories of cells with the manual counter fromthe Capture software, there are 7 different categories available.
Class Name: Double-click the category button to name the category personally.
Color: Double-click

to display the palette to choose the desired color.

Size: Double-click the number to set the size of points, the default is 10, the range is5-100.

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Figure 5-13

Add New Class: Click [Add New Class] to create a new category.
Delete Class: Click [Delete Class] to delete the selected category.
Undo: Click to undo the last operation.
Clear All: Click [Clear All] to clear all categories and clear the whole counter table
simultaneously.
Start Class Counting: Click [Start Class Counting] to select a certain category,click the left mouse button on the targets on image to count. The counted results will
be automatically display on the counting table.

Figure 5-14

After the counting with one or different categories, all the counting results data will be

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displayed on the counting table, select [Export to Excel], and export the data to the
Excel forms.

5.7 Scale Property
Mosaics software allows the user to set the scale properties for the user's actual
needs.

Figure 5-15

Show Scale: check on the blank to select to display the scale on the measured image
or not; the default setting is not showing the scale, if check to show the scale, thescale will automatically be shown on the left top of the image, use can use the mouseto drag the scale to the position he desires.
Type: select the display type of the current scale, support manual and automatic, thedefault is automatic;
Align: Set the alignment between the current scale and the value. It supports left,
center, and right alignment, and the default is center;
Orientation: Set the display direction of the current scale, support horizontal andPage 67

vertical, the default is horizontal;
Name: Create name for the scale applied o the current, the default setting is blank.
Length: The default value is 100 pixels, that is, the scale is displayed in total length of
100 unit pixels, and this value can be modified by entering new value.
Color: Choose the color for the current applied scale on the image, the default color isred, click on […] to choose the desired scale color.
Width: enter the value of width for the scale on applied the image, the default value is5, range 1~5.
Text Color: Choose the color for text of the scale currently applied on the image, thedefault color is red, click on […] to select the desired color.
Text Font: Choose the font format for scale currently applied on the image, the default
format is [Arial 28], click on […] to choose the desired format.
Border Color: Choose the color for border of the scale currently applied on the image,the default color is red, click on […] to select the desired color.
Border Width: enter the value of width of the scale currently applied on the image,the default value is 5, range 1~5.
No Background: check or uncheck the blank of True to set the background astransparent background, or with background for the scale currently applied on theimage, transparent background on the scale is the default setting.
Background Color: Select the background color for scale currently applied on theimage. Click on […] to choose the desired background color, the default backgroundcolor is white.
Apply to All: Apply settings to all scales.
Default: Resort and apply the default parameters settings to the scale on the image.

5.8 Ruler Property
Mosaic software allows the user to set the crosshair properties of the image according
to his actual needs.

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Figure 5-16

Show Ruler: check on the blank to select to display the cross ruler on the image ornot; the default setting is not showing the cross ruler.
Unit Interval: Set current applied cross ruler interval distance on the image;
Ruler Height: Set height of the currently applied cross ruler on the image
Ruler Color: Choose the color for the current applied cross ruler on the image, thedefault color is black, click on […] to choose the desired cross ruler color.
Ruler Font: Sets the font size on crosshair;
No Background: check or uncheck the blank of True to set the background astransparent background, or with background for the cross ruler currently applied on
the image, transparent background on the cross ruler is the default setting.
Background Color: Select the background color for cross ruler currently applied on
the image. Click on […] to choose the desired background color, the default
background color is white.
Default: Resort and apply the default parameters settings to the cross ruler on theimage.

5.9 Grid Property
Mosaics software allows the user to set the grid properties of the image according to
his actual needs.

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Figure 5-17

Show Grid: check on the blank to select to display the grid on the image or not; thedefault setting is not showing the grid.
Type: Select the way to set the grid to apply to the current image.
Row/Column: If you choose to define the line number, you can enter the numbers of
the line on rows and columns applied on the image. The default numbers of row gridand column grid are both 8.
Line Interval: If you choose to define the grid by the line interval, you can enter thenumber of grids you need into the blank of Line Interval, the default number of line
interval is 100.
Line style: Choose the line style for the grid to apply on the image there are 5 styles
of grid could be chosen from, the solid lines, dashed lines, dotted lines, dotted lines,
and two dotted lines.
Line Color: Choose the color for the grid to apply on the image, the default color isred, click on […] to choose the desired grid color.
Default: Resort and apply the default parameters settings to the grid on the image.

5.10 Save Settings
Copy the parameter file and load it on other computer, which can realize the transferof parameters across platforms and keep the user's experimental conditions as
consistent as possible.

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Figure 5-18

Group Name: Set the parameter name, it can also be viewed and loaded through thedrop-down menu.
Save: Save settings;
Load: Load selected settings;
Delete: Delete selected settings;
Export: Export selected settings;
Import: Import settings file into drop-down menu;
Reset All: Clear all user settings and restore to the software factory settings;

5.11 Fluorescence Intensity

Figure 5-19

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Mosaic allows users to measure the gray value of the image according to actual
needs, and supports both linear and rectangular methods;

Operating Procedures
1) Switch from the preview module to the measurement module, or open an image,and check [Start] to enable the function. At this time, the measurement tool is notallowed;
2) Draw a line segment or a rectangle to measure the fluorescence intensity of thedrawn area;
3) Click [Save] to save the current measurement data in excel format to the local
disk;

Note:


Draw a line segment graph and complete the drawing at the second click tomeasure the fluorescence intensity data on the straight lineTo draw a rectangle, left-click to select two endpoints to draw a rectangle graph,
which can measure the average fluorescence intensity data of each column ofpixels within the rectangle.

5.12 Cursor Property
The user can adjust the properties of the measurement cursor according to actual
needs. The setting interface is shown in Figure 5-22.

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Figure 5-20

Width: Used to set the thickness of the cross cursor line segment, the setting range is
1~5, and the default value is 2.
Cross style: Set the line style of the cross cursor, the default is solid line, and dottedline can be selected;
Cross length: Select the length of the cross cursor currently displayed on the image,the default is 100;
Pickbox length: Select the width and length of the cross cursor that is currently
displayed on the image, the default is 20;
Color: Select the currently applied cross cursor on the image to adjust the line color,select it and click the color palette to select the desired color;

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6. Report
Mosaics software provides report formats to export the measurement data to yourworking report documents, you can also export the report in real time when you in
preview window, In addition to the default template, a custom template is alsoavailable, and users can modify the report according to actual needs. Custom
template, only supports excel format.

6.1 Template Report
The software provides functions to export custom measurement templates,
measurement data modules and batch export reports.

Figure 6-1

Report Templates: Users can click on the name to select a template, and the default

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is the software template.
Add: Add a custom template, the custom template must be modified on the software
default template, the template format is Excel. The default template is in the[templates] file under the software installation path. Use the # identifier to indicate thecontent that needs to be displayed. When the ## identifier appears, it means that theheader of the data table is hidden.
Delete: Delete the selected template;
Open: Preview the selected template;
Export Report: Export the current report, the format is excel;
Batch Export: Check [Batch Export], the user can check the names of the pictures tobe exported, and click [Batch Export] to export the report. The image name supports
query.

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6.2 Report

Figure 6-2

Report Templates: Choose your desired format of report template.
Project Name: Enter your personal name for the project you are working on, thisname will show up on your working report,
Sample Name: Enter the name of your sample in this project, this name will show up
on your working report,
User Name: Enter the name of the user, or conductor, or worker, or reporter,
Notes: Enter the notes, supplement information or any or the comments you need toPage 76record down from reference for this job in this working report,
Image Name: Enter the name of the image you captured before, then the image can
automatically be loaded on the report.
Image information: Check on the blank to show the information of the image you justpulled out, un-check to vanish the information, the default setting is showing out the
information of the image on the report.
Measure Data: In the report, display/do not display the current image measure data,
default checked.
Count Table: In the report, display/do not display the current image count table,
default checked.
Export Report: Export current report into a document of with PDF format.
Print: Print the current report.
Cancel: Cancels this operation, it means clearing out all the report information justentered or loaded in.

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7. Display

Figure 7-1

Zoom In: Magnify the current image and let it display larger than its original size.
Zoom Out: Reduces the current image and let it display smaller than its original size.
1:1: The current displaying image is in its 1:1 original size.
Fit: Adjust the showing size of the image to fit the software operating window.
Black Background: The image is in full screen and the background of the image isdefault to be black. Type the [Esc] button of the computer keyboard or click on the
Back Arrow symbol on the right down corner of the screen to exit the black
background mode.
Full Screen: Enable the image to be full screen. Type the [Esc] button of thecomputer keyboard or click on the Back Arrow symbol on the right down corner of thescreen to exit the full screen mode.
Horizontal Flip: Horizontally mirrors the current image.
Vertical Flip: Vertically mirrors of the current image.
Rotate 90°: Rotate the current image clockwise, 90 degrees each click.

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8. Confi
8.1 Capture\Image\Measure
Software function modules and orders can be customized as shown below.

Figure 8-1

Shown: Check module to display on the software interface. Unchecking of the blanknext to the module means that the module will not be displayed on the interface. All
modules are checked by default. This setting can be saved after the software restarts;
Up: The shown module can be moved up, and the interface layout changes
accordingly to the moving.
Down: The shown module can be moved down, and the interface layout changes

Page 79accordingly to the moving.

8.2 Jpeg
Mosaic provides Jpeg format size settings. It can be operated in the settings. When
Jpeg is selected in the file saving module, the image size will be generated according
to the set format when taking pictures.

Figure 8-2

Default: When the default is selected, the generated image keeps the current camera
image resolution;
Resize: Check the resize to adjust the image size;
Percentage/pixel: Adjust pictures according to percentage/pixel. When a percentage
is selected, the adjustment range is 1-200%. When pixel is selected, the adjustment
range is 4-maximum resolution*2;
Horizontal: Set the size of the image in the horizontal direction;
Vertical: Set the size of the image in the vertical direction;
Keep Aspect Ratio: check it to keep the image aspect ratio to prevent image
distortion;

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9. Info
9.1 Preferences
Language: The software language can be selected. The software needs to berestarted to take the language setting into effect.

Figure 9-1

Microscope Option: To select applicable directions, software will take effect after
restart.

Page 81Figure 9-2

9.2 Help
The software operation instructions can be called out for the user to learn and refer.

9.3 About
Display company general contact information, as shown below.

Page 82

Figure 9-3

More information: such as the camera current working, the versions of the softwareand the information of the operating system.

Page 83

Figure 9-4

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10. Customer Services
Visit the official website of Tucsen which is www.tucsen.com and click on the [Support]
bar to get answers to frequently asked questions.

WarrantyThe product warranty is 24 months since the date of shipment. TUCSEN fulfill the
repairing to any damages corresponding with the warranty policies for free ofcharges.



The warranty policies are limited to the defects in manufacturing and materials of
the commodity. Anthropic damage such as self-disassembling, water ingressing,
dropping, and damages of natural disasters are not covered by the warranty.

Contact a professional to get technical support:

TEL: +86 591-88194580 ext:811

Email: service@tucsen.com

Or landing the official website to leave a message: http://www.tucsen.com

In order to save your time, please prepare the following information in advanced:



Camera model and S/N (product serial number);Software version number and computer system configuration information;

As details as possible of description of the problems and any images help toindicate and show to the problems.

www.tucsen.com

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