Ａｕｔｏｃｌａｖａｂｌｅ ＆ ＵＶ Ｒｅｓｉｓｔａｎｔ

Digital micro pipette for liquid handling

User’s Manual

*SB-NLT002ER 2022/6/10
SB-NLT001ER 2022/4/15

Thank you very much for purchasing Nichipet Premium LT.
Please read this manual carefully before using this device.
Safety Precautions





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

Please read this manual carefully and have sufficient understanding of thecontents and instructions, especially concerning matters of safety, prior touse.
The notes stated here is for the safety of the user, and for the correct
usage of the product.
Contents marked with
“Danger Level Symbols” are matters that
require the user’s utmost attention, not only for using Nichipet Premium LT
properly, but also to prevent users from injuries or death, harm to others,
and/or property damage.
After reading this manual, please keep it in a noticeable and accessible
place for ‘users of the device’ to refer to at any time.
Any serious incidents that has occurred in relation to the equipment shallbe reported to the manufacturer and to the competent authority of theMember State in which the user and/or the patient is established.

Danger Levels
DANGER

Will lead to serious injuries or death.

WARNINGMay lead to severe injuries or death.

CAUTION

May lead to light to moderate injuries, and/or cause
property damage.
User information

Caution on disposal of this product
When disposing the pipette body and tips (including adhering liquid), please
comply with the laws and regulations of each country related to disposal, or local
ordinance or regulation.

－ 1－

Autoclavable & UV res is t ant

Digital micro pipette for liquid handling

Table of Contents
1. Product overview
1.1 Features
1.2 Standard Accessories (Included)
1.3 Main illustration

3
3
3
4

2. Information on safety
2.1 Intended Use
2.2 Warnings for Intended Use
2.3 Material information

4
4
4
5

3. Operation/Operating procedure
3.1 Volume setting
3.2 Aspiration of liquid (Forward technique)
3.3 Dispensing the liquid
3.4 Recommendation for accurate pipetting (Technique)

6
6
7
9
10

4. Maintenance
4.1 Cleaning
4.2 Disassembling
4.3 Reassembling
4.4 Lubrication/Greasing
4.5 Autoclaving
4.6 Drying the pipette

11
11
11
12
14
19
19

5. Specifications (Accuracy/Precision)20

6. Calibration (Adjustments)
6.1 Volume adjustment method
6.2 Volume measurement/Inspection procedure

21
21
22

7. Troubleshooting24

8. Replacement Parts List
8.1 Consumables
8.2 Spare parts list

25
25
25
－ 2－

1. Product overview
1.1 Features
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Nichipet Premium LT is fully autoclavable at the condition of 121°C for 20 minutes.
Nichipet Premium LT is made of UV resistant materials, thus it can be used in a clean
bench environment.
Enhance UV resistance has been applied, but the surface of the pipette body might bedeteriorated by long time UV irradiation. It does not affect the accuracy and precision.
The newly designed curvature and roundness in shape is ergonomic, and mitigates theuser’s fatigue and stress levels from long periods of use.
Easy to read digital indicator.
The sample volume can easily be set by simply rotating the push button.
Setting the sample volume can easily be locked on with the one-touch lock mechanism.
A wide range of sampling volume can be covered by six models, from 0.1μL to 1,000μL.
Patented body construction shields the hand temperature permeating through the bodyand inner workings of the device, which increases the accuracy of the volume measurements.
Since highly durable PTFE (Fluoroplastic) is used in the product, long lasting accuracy
and precision is maintained.
A thin nozzle tip can be easily inserted, and can reach the bottom of universal test tubes(110mm in depth) for aspiration and dispensing of liquid.
The tip can be removed without direct contact, by simply pressing the tip ejector.
The Easy-Calibration function provides smooth and easy adjustment with the included
adjustment tool.

1.2 Standard accessories (Included)
Accessories

QTY
3

Tip
GreaseAdjustment tool

1
1

User’s Manual

1

When unboxing the package, check that all of the items above are included forthe respective model.

－ 3－

1.3 Main illustration

Push button
Lock lever
Ejector button
Volumeindicator

Main body
Ejector setting screw
Ejector pipe

2. Information on Safety
2.1

Intended Use

Nichipet Premium LT, used in conjunction with pipette tips recommended by Nichiryo,
are designed and constructed for low-contamination transfer of liquids, especially forsamples from the human body and for reagents within the scope of an in-vitro diagnostic
application in order to allow the in-vitro diagnostic medical device to be used as intended.
Therefore, Nichipet Premium LT is subjected to the accessories of in-vitro diagnostic
medical devices under Regulation (EU) 2017/746. The accessories is treated as in vitro
diagnostic medical devices in their own right under Regulation (EU) 2017/746.
Nichipet Premium LT are intended for operation by qualified staff.

2.2 Warnings for intended use
DANGER
When handling radioactive substances or infectious substances, always check andconfirm the information first, and follow guidelines on their safety procedures.
When using harmful liquids to the human body, be very careful in the handling of thesubstances.

Never touch or come into direct contact with the used chips.

Never touch filters directly that are contaminated by harmful or toxic substances.

When liquids that are harmful to the human body adheres and/or contamination occurs,
use appropriate measures to clean and decontaminate the device before continuing
its use.
This product is not intended for use on living organisms.

－ 4－

WARNING
Do not eject the tip with liquid inside of it.
Do not eject the tip towards anybody.
Do not expel or dispense any liquids towards anybody.
Depending on the splashed liquid, there is danger of causing injuries to the humanbody.
Please protect yourself in accordance with the general procedure of danger prevention,such as wearing protective clothing, protective glasses and gloves.
CAUTION
Do not use the pipette for any other purpose, use only for pipetting and liquid dispensing.
Do not modify the pipette, modification can lead to accidents.
Do not stab the tip into the human body, or eject the tip towards anyone. The tip isvery sharp and extremely dangerous.
Since the main body of the pipette becomes extremely hot right after autoclaving anddrying. In this state, please do not touch it directly with your hands. It can lead to accidents
and burn injury.
Do not use the pipette for any purpose other than pipetting and liquid dispensing,such as stirring liquid with this product. It can lead to loosening of the tip, the tip
dropping off, liquid adhesion and contamination to the main unit of the device andaccidents and/or injury.
During operation and maintenance, if any worn, missing or broken parts are detected,
discontinue use immediately. Order and replace the part or parts, before use.



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

2.3

Material information

CAUTION

The use of highly reactive chemicals may damage the device. Please acknowledge
the following materials that are used, and do not use liquids that will violate them.

Material of external parts
 Polypropylene (PP) Polyetherimide (PEI) modified- Polyphenyleneether (m-PPE) Polyetheretherketone (PEEK) Fluororubber (FKM) Stainless steel Aluminum alloy Alumina－ 5－

* 3. Operation/Operating procedure









Users of the device are required to strictly observe the following in orderfor the pipette to keep its excellent accuracy, precision and original
performance.
Do not expose pipette to direct sunlight when using it, or for 2 hours prior to use,otherwise the pipette may lose its accuracy. Avoid working with pipettes in a hightemperature, low temperature, low humidity environment, or when thetemperature difference between the environment including the main body of thedevice and the liquid is large, accuracy and precision may not be guaranteed.
This Pipette can be used in a stable environment between +4°C and +40°C, but
the specifications may vary.
Just prior to use, avoid touching the tip or nozzle cylinder as much as possible. Ifthey are warmed up, accuracy may not be obtained.
Original Nichiryo tips are recommended. Nichipet Premium LT is calibrated with theoriginal Nichiryo tip(Premium Tip ), if you use other tips, deviations in original factory settings may occur, and accuracy will not be guaranteed.
Depending on the frequency of use, the pipette should be cleaned and the airtightchamber should be maintained according to the manual.

3.1 Volume setting1) Turn the lock lever to the unlocking direction to loosen it (Fig. A).
2) Turn the push button to set the volume indicator to a desired liquid volume.
When setting the liquid volume, set the indicator’s minimum graduation scale to thetriangle point marker in the lower part of the volume indicator (Fig. B). Please refer tothe volume indicator and the minimum graduation unit for each model to set themeasurement volume accordingly. The volume indicator numbers are colored in black,or in red, to indicate the position of the decimal point. Black indicates the integer, red
indicates the decimal. Scale units are in (μL).
3) To increase the volume setting, turn the push button till it passes the designatedvolume setting by at least half a rotation of the push button dial, and then dial back to set
the designated volume.
4) To decrease the volume setting, simply turn it to the designated volume directly.
5) After setting the liquid volume, turn the lock lever to the locking position to lock it(Fig. A).

CAUTION

Do not exceed the specified liquid volume limit, otherwise the pipette may bedamaged or deteriorate in its quality.

－ 6－

Lock
Push button

UnlockLock lever
Ejector button Minimum
graduation unit

Fig. A
Volume indicator
Model: NLT-2
2μL

10μL
NLT-1020μL
NLT-20100μL
NLT-100

200μL
NLT-200 1000μL
NLT-1000

Point mark2μL
( 0. 002)

10μL( 0. 01)

20μL( 0. 02)

100μL( 0. 1)200μL 1000μL
Minimum( 0. 2)( 1)
graduation unit

Fig. B

3.2 Aspiration of liquid (Forward technique)1)

Attach a new tip to the nozzle end.



It is recommended that tips are directly picked up from the rack. Do not twist
the pipette when fixing tip on.
Please be sure to mount the tip on the main nozzle securely. Failure to do so
may cause the tip to drop off and liquid to splash.

CAUTION

Do not perform pipetting with less liquid than the set volume. If the quantity of liquid isless than the set volume, it may cause the liquid to spray into the main body, and thepipette may be damaged or deteriorate in its quality.
2)
3)

Press down the push button fully to the first stop position “b” (Fig. C) – ①.
Hold the pipette vertically and immerse the tip 2mm. to 3mm. below the surface ofthe liquid (Fig. D) - ①.
4) To aspirate the set volume of liquid into the tip, release the push button slowly andlet the push button go back to the initial position naturally. It takes 1 second to－ 7－

aspirate the liquid. During this operation, stop to wait for the suction process of theliquid to be totally completed, making sure that the liquid is drawn up into the tip
with certainty (Fig. D) -②.
5) Draw the tip of the pipette carefully vertically upward and away from the liquidsurface, then touch the tip to the side of the tube to remove excess droplet adheringto the outside of the tip (Fig. D) - ③.

①

②

③

④

a
b
c

Ejector button

Fig. C

Do not aspirate when the push button is at ‘position ④’ (Fig. C).
We recommend using the forward technique as the operation method of thisdevice for pipetting. Nichipet Premium LT has been calibrated with the forward
method, and precision may not be obtained when dispensing by any othermeans.
Always change the tip when using different liquids to avoid cross-contamination.

CAUTION
Please operate the push button slowly and gently.
Sudden release of the push button, can lead to the aspiration of the liquid into the nozzle
cylinder; not only precise accuracy will not be obtained, but also the quality of the device
will be impaired. Due to the larger base volume measurements of models, 1000μL,
there is a higher chance of the above to occur.
When using the pipette, be sure to always attach and use a tip. Failure to do so, willlead to liquid entering the inside of the device’s nozzle cylinder and cause serious
malfunctions
 Never turn the device sideways or upside down with liquid in the tip. Liquid can enter
inside the device’s nozzle cylinder, which can cause ※contamination and breakdown.
※Cross-contamination with the entered liquid remaining inside the main body of the device

can also occur, when switching liquids thereafter.

－ 8－

3.3 Dispensing the liquid
1) Touch the end of the tip against the inside wall of the recipient tube at a slight angle.
2) Press the push button down slowly and smoothly to the first stop “position b”.
Wait for a second, then press the push button down to the second stop “position c” toexpel the last droplet of liquid from the tip (Fig. D) - 4 and 5.
3) Keep the push button compressed. Remove the last droplet by slightly touching andrunning the end of the tip up against the inner wall of the tube, then move the tip of
the pipette vertically straight up and out of the tube.
4) Press the ejector button to detach the tip to dispose (Fig. D) – 6.
a

We recommend the tip to be disposed of after each use.
Repeated use may lead to and result in; loss of accuracy, precision, and maylead to contamination/cross-contamination due to adhesion and deposits fromprior use.

①

②

③

b
c

Fig. D

－ 9－

④

⑤

⑥

3.4 Recommendation for accurate pipetting (Technique)
In addition to the previously mentioned operations of pipetting, the following
technique maximizes the performance of the pipette.
1) Pre-Rinsing the tip
Higher precision can be obtained by performing the pre-rinsing of the tip. When using a
fresh pipette tip for the first time and before sampling, it is important to pre-rinse the tip
at least two to three times with the target solution before pipetting. Repeat the aspirationand discharge to the position of the 1st stop consecutively for two to three times(Fig. E) – b.
When strict precision is required, this method is recommended for all kinds of liquidhandling. Pre-rinsing is a fast and easy way to increase the accuracy.
2) Handling of High Density Solutions / Viscous Solutions
After aspirating the liquid into the tip, wait for 2 to 3 seconds before removing the tip slowly
from the surface of the liquid. When dispensing, wait 2 to 3 seconds at the first stop
position before pushing into the second stop position (Fig. E) – c.
3) Small volume dispensing
Especially for volumes less than 50μL, please operate the pipette slowly and smoothly.
Also, please pay close attention to the effect of evaporation loss, due to temperature andhumidity.

abcFig. E－ 10－

4. Maintenance
4.1 Cleaning
When Nichipet Premium LT’s exterior is soiled, please use 70% ethanol on a clean soft cloth
to wipe it off.
Also, if any symptom that is described in the "7. Troubleshooting" section occurs, disassemble
and inspect the device (each part), and then check and follow the procedures in the‘Troubleshooting Table’ to identify and isolate, correct and/or fix the situation or problem.

CAUTION

Use of highly reactive liquids, may damage the device.
After using a highly reactive liquid or/and if contamination occurs, perform the disassemblyand cleaning of the device, accordingly to the following method in this manual.

In order to keep and use Nichipet Premium LT in its best condition, we
recommend the user carries out; periodic checks and maintenance, and volume
inspection/calibration of the device.

4.2 Disassembling
1)
2)

Turn counterclockwise to loosen and remove the ejector setting screw.
(6 Models) - 2μL to 1000μL (Fig. F) - (1-1)
・6 Models ranging from 2μL to 1000μL (Fig. F) - (1-2)
The nozzle cylinder(outer threading) screws into the main body.

（１ －１）⑧Ejector setting screw⑦Ejector pipe

（１ －２）⑤Nozzle cylinder

Fig. F
Circled Numbers in (Fig. F) corresponds to Parts Numbers in the replacement parts list.

－ 11－



3)

CAUTION
When removing the nozzle cylinder, the internal parts may spring out and apart fromthe reaction of the loaded spring inside, be mindful of this point, and take precautions
when unscrewing and taking apart the nozzle cylinder.
Disassemble the internal parts as shown in; (Fig. H1) for Models: 2μL,10μL, (Fig. H2)
for Models: 20μL, and (Fig.H3) 100μL and 200μL.
・For Models: 2μL and 10μL, remove the plunger, 1st spring, O-ring retainer in the correctand stated order. The O-ring and the seal ring cannot be detached. They are press-fitted
and built into the structure as an integral part of the nozzle cylinder.
Please take out only the 1st spring and the O-ring retainer.
・For Models: 20μL, remove the plunger, 1st spring, 1st spring holder, seal spring,O-ring retainer in the correct and stated order. The O-ring and the seal ring cannot be
detached. They are press-fitted and built into the structure as an integral part of thenozzle cylinder.
Please take out only the 1st spring，1st spring holder, seal spring and the O-ringretainer.
・For Models: 100μL, 200μL and 1000 μL, remove the plunger, 1st spring, 1st spring
holder, seal spring, O-ring retainer and O-ring in the correct and stated order.
Please be careful not to lose any small parts during disassembly.



Depending on the Model, the specification of the O-ring retainer will be different.

4.3 Reassembling
1)
2)

If lubrication is required, apply grease to the plunger and O-ring.
Please refer to: 4.4 Lubrication (Greasing the plunger and the nozzle cylinder.)Reassembling the nozzle cylinder
・For Models: 2μL, 10μL, put the plunger set into the first spring and set the combinedparts into the O-ring retainer, then add the seal ring and O-ring in the stated order.
Insert the whole assembly into the nozzle cylinder and screw it into the main body.
・For Models: 20μL, put the plunger set into the first spring and set the combined parts
into the first spring holder, then add the seal spring in the stated order. Insert thewhole assembly into the nozzle cylinder and screw it into the main body.


There are very rare instances in which the O-ring and seal ring may come off
during disassembly of the 2μL, 10μL and 20μL models, hence we have shown
the respective parts detached and separated on (Fig. H1) and (Fig. H2).
Assembling the seal ring and the O-ring in the reverse/opposite order will leadto; loss of accuracy and precision, not being able to aspirate the liquid and/orleakages.
Please always check the presence of the O-ring and the seal ring during
disassembly and assembly.

－ 12－

・For models: 100μL and 200μL (Fig. H2), 1000μL (Fig. H3)
Put the plunger set into the first spring and set the combined parts into the first spring
holder, then add the seal spring, O-ring retainer and the O-ring in the stated order.
Insert the whole assembly into the nozzle cylinder and screw it into the main body.
3)

When screwing the nozzle cylinder into/onto the main body, hold the parts
vertically as a visual reference, and slowly turn to let the threads bite together
correctly. If screwed in an offset way or with force, the O-ring will be unseated,
stripping of the threads and/or damage to the casing can occur, all of which, willcause leakage.

Fitting the ejector pipe to the main body.
・Models: 2μL, 10μL (Fig. H1), 20μL(Fig.H2),100μL and 200μL (Fig. H3),1000μL (Fig. H4)
Press the ejector button down with your thumb to help meet and align with theposition to secure, insert the ejector pipe onto the body and secure it with theejector setting screw. If resistance is felt when ejecting, jiggle the ejector pipe backand forth very slightly, to give it better alignment, response and action (Fig. J).
After reassembling, perform several tests to inspect the pipette’s function/reliability.

－ 13－

4.4 Lubrication/Greasing
1) For the 2μL, 10μL and 20μL models, remove the old grease from the plunger. For the
100μL, 200μL and 1000μL models, remove the old grease from the plunger and O-ring.
2) Please apply a thin layer of the supplied grease, from the tip of the plunger to about
40mm up (Fig. G – the part shown in gray).



CAUTION
Always used the supplied grease.
Using other than the supplied grease may lead to damage of the device, and will not
be covered by the warranty.


We recommend the lubrication/greasing of the device, at least once every six
months.

2μL
10μL
20μL

100μL
200μL

1000μL

Fig. G－ 14－

O-ringretainer

Main body

Seal ringO-ring⑤NozzlecylindersetNozzlecylinder①Plunger set⑧Ejector setting screw②1st spring⑦Ejector pipe

Fig. H1
Circled Numbers in (Fig. H1) corresponds to Parts Numbers in the replacement parts list.
－ 15－

20μL

O-ringretainer

Main body

Seal ringO-ring⑤NozzlecylindersetNozzlecylinder①Plunger set②1st spring③1st spring
holder⑧Ejector setting screw④Seal spring⑦Ejector pipe

Fig. H2
Circled Numbers in (Fig. H2) corresponds to Parts Numbers in the replacement parts list.
－ 16－

100μL / 200μL

O-ringretainer⑥O-ring set

Main bodyO-ring⑤Nozzle cylinder①Plunger set②1st spring③1st spring
holder⑧Ejector setting screw④Seal spring⑦Ejector pipe

Fig. H3
Circled Numbers in (Fig. H3) corresponds to Parts Numbers in the replacement parts list.
－ 17－

1000μL

Main body⑤Nozzle cylinder①Plunger set②1st spring⑧Ejector settingscrew③1st spring
holder④Seal spring⑥O-ring set⑦Ejector pipe

O-ringretainer
O-ringFig. H4
Circled Numbers in (Fig. H4) corresponds to Parts Numbers in the replacement parts list.
－ 18－

4.5 Autoclaving
The whole pipette is autoclavable. Carry out the autoclaving process at 121°C for 20
minutes, following the procedure below.
1) When autoclaving, always leave the lock mechanism loosened, and always check and be
sure to set the volume indicator to the ‘Maximum Volume’ of the pipette’s volume
range. (This moves the plunger back/in.)2) Start the autoclaving process. After autoclaving is done, please promptly dry out thepipette with the following procedure.

WARNING

Due to high temperature and high pressure use in autoclave sterilization, it is very
dangerous. Please operate equipment according to safety guidelines and standards.

CAUTIONWhen autoclaving, do not stack items on top of each other, do not place the nozzle
downward, or lean the device diagonally against anything, as this will apply load andstress to the heated structure.
Always autoclave the pipette with the nozzle facing upward and standing.
Although the pipette is made of autoclave compatible material, due to the hightemperature and pressure used in the sterilizer, there is a risk that heated parts
subjected to load and stress to deform.
Do not autoclave at temperatures above 121°C (There is a risk of causing damage.)

4.6 Drying the pipette
1) As the same as autoclaving the device, unlock and loosen the lock lever when drying.
2) Remove the ejector pipe following the procedure in 4.2 Disassembling (Fig. F).
3) Turn the nozzle cylinder counterclockwise by two and a half turns to just loosen it.
4) Dry it with a blower type constant temperature dryer, and confirm that it is completely
dried out.
5) After drying, wait until the main unit has returned to room temperature, tighten thenozzle cylinder, and reattach the ejector pipe back into/onto the main body.



CAUTION
Immediately after autoclaving and drying, the pipette and parts are extremely hot, so
please do not touch them directly with your hands.
When autoclaving, there is a possibility of damage, and performance may be affected,
due to other items that are in the autoclave at the same time, and/or by substancesthat may be present in the water that is being used in the autoclave.
Assembling the pipette in a heated or warm condition after drying, can lead to damage,such as stripping of the threads. Please let it cool down first. Also, if used in a heated or
warm condition, accuracy will not be obtained.



Accuracy and precision may change with the autoclaving conditions, and alsowith many other various factors. We recommend testing the accuracy andprecision after autoclaving, and at other times when needed.

－ 19－

* 5. Specifications (Accuracy/Precision)

Table-1

Maximum Permissive Errors.

Models(Code)

Volume range

2μL(00-NLT-2)

0.1-2
(μL)

10μL
(00-NLT-10)

0.5-10(μL)

20μL(00-NLT-20)

2-20
(μL)

100μL
(00-NLT-100)

10-100(μL)

200μL(00-NLT-200)

20-200(μL)

1000μL(00-NLT-1000)

100-1000(μL)

Volume(μL)

Accuracy(systematic error)
AC (%)

Precision(random error)
CV(%)

0.2
1
2
1
5
10
2
10
20
10
50
100
20
100
200
100
500
1000

±12.0★
±5.0
±3.0
±4.0
±1.0
±1.0
±5.0
±1.0
±1.0
±2.0
±1.0
±0.8
±1.0
±0.8
±0.8
±1.0
±0.8
±0.7

≤6.0★
≤2.5
≤1.0
≤3.0
≤1.0
≤0.5
≤3.0
≤1.0
≤0.4
≤1.0
≤0.3
≤0.3
≤0.5
≤0.3
≤0.2
≤0.5
≤0.3
≤0.2

Accuracy and precision of 0.2μL or less of “2μL model” are greatly affected by the user’s
sampling skills, and/or by various factors and conditions.
★

・
・
・
・
・

Tips used: Premium Tip (BMT2)
Measurement temperature: Between 20°C to 25°C
Relative humidity: above 50%
Measurement medium: distilled water
Barometric pressure, 101kPa
Volume measurement is in accordance with ISO 8655-6.

－ 20－

6. Calibration (Adjustments)
Please have adequate foreknowledge to responsibly perform all operations.
We cannot guarantee, accuracy/precision, or trouble in pipetting operation, after the user has
arbitrarily performed any adjustments after factory settings.
We thank you in advance for your understanding.
Perform the volume adjustment and volume inspection in the following method.

6.1 Volume adjustment method
To perform a good and reliable calibration for all the models of pipettes in this manual,
conduct the adjustment at minimum volume setting first, and then conduct the maximum
volume setting adjustment.
Please perform the volume check of the maximum volume after the minimum volume
accuracy falls within the standard accuracy on [Table 1].
Volume adjustment procedure
1) Loosen and unlock the lock mechanism.
2) Insert the adjustment tool’s prongs into the two grooves (Fig. L①).
3) Hold the unit in one hand and make sure the adjustment tool is fixed in (Fig. L②).
4) Rotate clockwise to increase the dispensing volume, and counterclockwise to
decrease the dispensing volume.
5) Please refer to [Table 2] for the required fine adjustment of the volume.

Adjustment tool

Clockwise:
Dispensing volume increase
Counterclockwise:
Dispensing volume decrease

Adjustment tool①②

Fig. L

Circled Numbers in (Fig. L) corresponds to Parts Numbers in the replacement parts list.

－ 21－

Table-2
Models
2μL
10μL
20μL
100μL
200μL
1000μL

Calibration guideline
１ scale mark0.00150.0077
0.012
0.078
0.13
0.79

2 scale marks
0.00290.0154
0.025
0.156
0.25
1.58

5 scale marks
0.00730.0385
0.062
0.390
0.63
3.96

10 scale marks
0.01450.0769
0.125
0.780
1.26
7.91

The numerical values (volume) above are for reference only. For actual measurement, pleaseuse a balance which is properly calibrated.

6.2 Volume Measurement/Inspection Procedure* 1)

In order to avoid influence from temperature differences, ready the pipettes forinspection, the distilled water, the balance and tips 2 to 3 hours before use, in theenviron where the measurement is to be conducted to attain temperature equilibrium.
※ The measurement room should be in a controlled temperature between 20°C to
25°C, and the measurement should be held where there is no direct influence from windblowing from an air-conditioner, heater or fan.
Please use the Nichiryo Original Premium Tip Series (volume compatible ones), and be
sure to install it on the pipette from the rack.

2)

Using distilled water as the sample, aspirate and dispense the volume to be calculated
inside the weighing vessel on the balance. Please use a vessel with a lid for theweighing vessel inside the balance. Also, include a small amount of distilled water in thevessel, prior to adjusting the balance and measurement.

3)

Read the mass (mg) measured with the balance, and then convert it with the"Z correction Factor for Distilled Water[table3]" to obtain the dispensed measured volume (μL).

Vi  mi  Z
4)Vi: Volumem i : Measured massZ

: Z correction factor

Add together the 10 volumes delivered and divide the sum by 10 to provide the mean
volume.

n1V   Vi
10 i 1－ 22－

V : Mean volume

5)

Calculate the systematic error es [%] with the equation below.

es  100 
6)V  V e s : systematic error [%]V s : selected volumes

Vs

Calculate the random error CV [%] with the equation below. Where n is the number ofmeasurements, in this case n=10 .

 V  V 
n

CV 

100

V

i 1

2

i

CV : random error [%]n 1

Table-3 Z correction factor for distilled water
Air Pressure (kPa)
Temperature
(°C)
95
100
101.3
20.0
1.00281.00281.002920.5
1.00291.00291.003021.0
1.00301.00311.003121.5
1.00311.0032
1.003222.0
1.0032
1.00331.003322.5
1.00331.00341.003423.0
1.00341.00351.003523.5
1.00361.00361.003624.0
1.00371.00371.003824.5
1.00381.00391.003925.0
1.00391.00401.0040－ 23－

105
1.00291.00301.00311.0032
1.00331.00341.00361.00371.00381.00391.00407. Troubleshooting
Symptom
Tip cannot beejected.

Pipette fails toaspirate liquid.

Possible cause

RemedyThe nozzle cylinder is loose

Securely, tighten up the nozzle
cylinder.

Buildup of matter/substance on theinside of the cylinder wall.

Clean or replace the nozzle
cylinder.

The nozzle cylinder is loose

Securely, tighten up the nozzle
cylinder.

Seal ring set are assembled inreverse order.
(2, 10, 20μL models only)

Reset the seal ring and O-ring incorrect order. Refer to;“Disassembling/Reassembling”.

The seal ring and/or O-ring set isworn.
(For 2, 10, 20μL models only).

Replace the O-ring set.
For 2μL, 10μL, 20μL model,
replace the nozzle cylinder set.

Grease on plunger set and/or O-ring Apply grease by following theis gone.
instruction on this manual.
The seal ring and/or O-ring set isworn.
(For 2, 10, 20μL models only).

Replace the O-ring set.
For 2μL, 10μL, 20μL model,
replace the nozzle cylinder set.

The nozzle cylinder is loose

Securely, tighten up the nozzle
cylinder.

Extracted liquid
Noticeable wear to the nozzle cylinder. Replace the nozzle cylinder.
leaks from the tip.
The plunger is damaged or rusty.
Replace the plunger set.
The tip is loosely attached.

Reattach the same loosened tip,or with a new one firmly.

Grease on plunger set and/or O-ring Apply grease by following theis gone.
instruction on this manual.

Push button
moves stiffly.

The liquid has aspirated and leaked
inside the nozzle cylinder.

If the push button does not work
well after dispensing, or it is sticking
to the body, disassemble the pipette
and wash/clean every part (orwipe down every part with a soft
cloth). If some parts get rusty or
corroded inside the body, replace
the part(s) with new one(s).

Grease on plunger set and/or O-ring Apply grease by following theis gone.
instruction on this manual.

When the pipette cannot be fixed after examining and conducting the above
mentioned procedure, immediately stop using the pipette and ask us or our
agent to repair it.
Before bring the pipette for repair, be sure to check whether it has been
contaminated with microbes, and/or harmful or toxic substance.

－ 24－

8. Replacement parts list
* 8.1 Consumables● Premium Tip (Bulk tip, Autoclavable)
Code

Volume range
(μL)

Color

ApplicablemodelsQ’ty

00-BMT2-UT

0.1-10

Clear

2μL

1000

00-BMT2-SSW

0.5-10

Clear

10μL

1000

00-BMT2-SG

2-200

Clear

20/100/200μL

100000-BMT2-LG

100-1000

Clear

1000μL

1000

ApplicablemodelsQ’ty● Premium Tip (Racked tip, Autoclavable)
Code

Volume range
(μL)

Color

00-BMT2-UTWR

0.1-10Clear

2μL

960 (96pcs x 10 cases)

00-BMT2-SSWR

0.5-10

Clear

10μL

960 (96pcs x 10 cases)

00-BMT2-SGR

2-200

Clear

20/100/200μL

960 (96pcs x 10 cases)

00-BMT2-LGR

100-1000

Clear

1000μL

960 (96pcs x 10 cases)

8.2 Spare parts list
Content

Type in volume① 00-NLT-0100002 Plunger set 2μL

Plunger head/plunger

2μL① 00-NLT-0100010 Plunger set 10μL

Plunger head/plunger

10μL① 00-NLT-0100020 Plunger set 20μL

Plunger head/plunger

20μL① 00-NLT-0100100 Plunger set 100μL

Plunger head/plunger

100μL① 00-NLT-0100200 Plunger set 200μL

Plunger head/plunger

200μL① 00-NLT-0101000 Plunger set 1000μL

Plunger head/plunger

1000μL② 00-NLT-0200002 First spring 2μL------

2μL② 00-NLT-0200010 First spring 10μL------

10μL② 00-NLT-0200020 First spring 20μL------

20μL② 00-NLT-0200100 First spring 100μL------

100μL② 00-NLT-0200200 First spring 200μL------

200μL② 00-NLT-0201000 First spring 1000μL------

1000μL

Code

Description－ 25－

Content

Type in volume③ 00-NLT-2300020 1st springholder 20μL------

20μL③ 00-NLT-2300100 1st springholder100μL------

100μL③ 00-NLT-2300200 1st springholder 200μL------

200μL③ 00-NLT-2301000 1st springholder1000μL------

1000μL④ 00-NLT-2200020 Seal spring 20μL------

20μL④ 00-NLT-2200100 Seal spring 100μL------

100μL④ 00-NLT-2200200 Seal spring 200μL------

200μL------

1000μL

Code

Description④ 00-NLT-2201000 Seal spring 1000μL⑤ 00-NLT-0400002 Nozzle cylinder set 2μL⑤ 00-NLT-0400010 Nozzle cylinder set 10μL

O-ring/seal ring/O-ringretainer/nozzle cylinder
O-ring/seal ring/O-ringretainer/nozzle cylinder

2μL
10μL

⑤ 00-NLT-0400020 Nozzle cylinder set 20μL

O-ring/seal ring/O-ringretainer/nozzle cylinder

20μL⑤ 00-NLT-0400100 Nozzle cylinder 100μL------

100μL⑤ 00-NLT-0400200 Nozzle cylinder 200μL------

200μL⑤ 00-NLT-0401000 Nozzle cylinder 1000μL------

1000μL⑥ 00-NLT-0500100 O-ring set

100μL

O-ring/O-ring retainer

100μL⑥ 00-NLT-0500200 O-ring set

200μL

O-ring/O-ring retainer

200μL⑥ 00-NLT-0501000 O-ring set 1000μL

O-ring/O-ring retainer

1000μL⑦ 00-NLT-0900010 Ejector pipe A------

2/10μL⑦ 00-NLT-0900200 Ejector pipe B------

20/100/200μL------

1000μL⑧ 00-NLT-0801000 Ejector setting screw set

Setting screw/spring
washer/washer

2/10/20/100/
200/1000μL⑨ 00-NLT-1600000 Volume adjustment tool------

All⑩ 00-NLT-3400000 Grease------All⑦

00-NLT09001000

Ejector pipe C

Please note that the specifications of the accessories may be changed without notice.
Always check our website for the latest specifications and information.
※Copying/Reprinting the manual in whole or in part without permission, is prohibited by law.

－ 26－

Inspection and Calibration Statement
The enclosed pipette was tested and calibrated under closely controlled environmental conditions
to ensure that it meets published calibration specifications. The precision and accuracy results
obtained for this pipette are provided on the enclosed calibration certificate. Because temperatureand humidity conditions affect the calibration results of liquid measurement devices, yourpipette should be calibrated under conditions of use. The calibration results obtained in yourlaboratory may vary from our results, due to differences in the environmental testing conditions.

Information on repair or servicing, when contacting your local distributor.

Authorized Representative：
Emergo Europe B.V.
Prinsessegracht 202514 AP, The Hague The Netherlands

Manufacturer：

Head office & Factory
2760-1 Nishikata, Koshigaya-Shi, Saitama 343-0822, Japan
TEL: +81-48-989-1301 FAX: +81-3-5829-4504
Tokyo office (Sales Division)
PMO Kandaiwamoto-cho Ⅱ 4F, 2-2-4 Iwamoto-cho, Chiyoda-ku,
Tokyo 101-0032, Japan
TEL: +81-3-5829-4539 FAX: +81-3-5829-4504
URL: http://www.nichiryo.co.jp/
E-mail: i-sales@nichiryo.co.jp