Ａｕｔｏｃｌａｖａｂｌｅ ＆ ＵＶ ＲｅｓｉｓｔａｎｔNichipet EX Plus II
Digital micro pipette for liquid handling

User’s Manual

*SB-NPLOII002ER 2022/6/10
SB-NPLOII001ER 2022/4/15

Thank you very much for purchasing Nichipet EX Plus II.
Please read this manual carefully before using this device.
Safety Precautions
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Please read this manual carefully and have sufficient understanding of thecontents and instructions, especially concerning matters of safety, prior touse.
The notes stated here is for the safety of the user, and for the correct
usage of the product.
Contents marked with
“Danger Level Symbols” are matters that
require the user’s utmost attention, not only for using Nichipet EX Plus IIproperly, but also to prevent users from injuries or death, harm to others,
and/or property damage.
After reading this manual, please keep it in a noticeable and accessible
place for ‘users of the device’ to refer to at any time.
Any serious incidents that has occurred in relation to the equipment shallbe reported to the manufacturer and to the competent authority of theMember State in which the user and/or the patient is established.

Danger Levels
DANGER

Will lead to serious injuries or death.

WARNINGMay lead to severe injuries or death.

CAUTION

May lead to light to moderate injuries, and/or cause
property damage.
User information

Caution on disposal of this product
When disposing the pipette body and tips (including adhering liquid), please
comply with the laws and regulations of each country related to disposal, or local
ordinance or regulation.

－ 1－

Autoclavable & UV res is t ant

Nichipet EX Plus II
Digital micro pipette for liquid handling

Table of Contents
1. Product overview
1.1 Features
1.2 Standard Accessories (Included)
1.3 Main illustration

3
3
3
4

2. Information on safety
2.1 Intended Use
2.2 Warnings for Intended Use
2.3 Material information

4
4
4
5

3. Operation/Operating procedure
3.1 Volume setting
3.2 Aspiration of liquid (Forward technique)
3.3 Dispensing the liquid
3.4 Recommendation for accurate pipetting (Technique)

6
6
7
9
10

4. Maintenance
4.1 Cleaning
4.2 Disassembling
4.3 Reassembling
4.4 Changing the Nozzle Filter
4.5 Autoclaving
4.6 Drying the pipette

11
11
11
12
20
21
22

5. Specifications (Accuracy/Precision)23

6. Calibration (Adjustments)
6.1 Volume adjustment method
6.2 Volume measurement/Inspection procedure

24
24
25

7. Troubleshooting27

8. Replacement Parts List
8.1 Consumables
8.2 Spare parts list

28
28
29
－ 2－

1. Product overview
1.1 Features
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Nichipet EX Plus II is fully autoclavable at the condition of 121°C for 20 minutes.
An O-ring made of perfluoro rubber is used in the airtight compartment, which gives thisdevice excellent thermal and chemical resistance, and organic solvent compatibility.
Nichipet EX Plus II is made of UV resistant materials, thus it can be used in a clean
bench environment.
Enhance UV resistance has been applied, but the surface of the pipette body might bedeteriorated by long time UV irradiation. It does not affect the accuracy and precision.
The newly designed curvature and roundness in shape is ergonomic, and mitigates theuser’s fatigue and stress levels from long periods of use.
Easy to read digital indicator.
The sample volume can easily be set by simply rotating the push button.
Setting the sample volume can easily be locked on with the one-touch lock mechanism.
A wide range of sampling volume can be covered by eight models, from 0.1μL to
10,000μL.
Patented body construction shields the hand temperature permeating through the bodyand inner workings of the device, which increases the accuracy of the volume measurements.
Since highly durable PTFE (Fluoroplastic) is used in the product, long lasting accuracy
and precision is maintained.
A thin nozzle tip can be easily inserted, and can reach the bottom of universal test tubes(110mm in depth) for aspiration and dispensing of liquid. (2μL – 1000μL)
The tip can be removed without direct contact, by simply pressing the tip ejector.

1.2 Standard accessories (Included)
Accessories
Tip（2μL/10μL/20μL/100μL/200μL/1000μL）
Tip（5000μL/10000μL）

QTY
3

Nozzle filter (1000μL/5000μL/10000μL)

1
3Nozzle filter remover(1000μL/5000μL/10000μL)

1

Adjustment tool

1

User’s Manual

1

When unboxing the package, check that all of the items above are included forthe respective model.

－ 3－

1.3 Main illustration

Push button
Lock lever
Ejector button
Volumeindicator

Main body

Ejector setting screw
Ejector pipe

2. Information on Safety
2.1

Intended Use

Nichipet EX Plus II, used in conjunction with pipette tips recommended by Nichiryo, aredesigned and constructed for low-contamination transfer of liquids, especially for samples from the human body and for reagents within the scope of an in-vitro diagnostic
application in order to allow the in-vitro diagnostic medical device to be used as intended.
Therefore, Nichipet EX Plus II is subjected to the accessories of in-vitro diagnostic
medical devices under Regulation (EU) 2017/746. The accessories is treated as in vitro
diagnostic medical devices in their own right under Regulation (EU) 2017/746.
Nichipet EX Plus II are intended for operation by qualified staff.

2.2 Warnings for intended use
DANGER
When handling radioactive substances or infectious substances, always check andconfirm the information first, and follow guidelines on their safety procedures.
When using harmful liquids to the human body, be very careful in the handling of thesubstances.

Never touch or come into direct contact with the used chips.

Never touch filters directly that are contaminated by harmful or toxic substances.

When liquids that are harmful to the human body adheres and/or contamination occurs,
use appropriate measures to clean and decontaminate the device before continuing
its use.
This product is not intended for use on living organisms.

－ 4－

WARNING
Do not eject the tip with liquid inside of it.
Do not eject the tip towards anybody.
Do not expel or dispense any liquids towards anybody.
Depending on the splashed liquid, there is danger of causing injuries to the humanbody.
Please protect yourself in accordance with the general procedure of danger prevention,such as wearing protective clothing, protective glasses and gloves.
CAUTION
Do not use the pipette for any other purpose, use only for pipetting and liquid dispensing.
Do not modify the pipette, modification can lead to accidents.
Do not stab the tip into the human body, or eject the tip towards anyone. The tip isvery sharp and extremely dangerous.
The filter replacement tool tip is very sharp and can be dangerous. Please handle itwith caution.
Since the main body of the pipette becomes extremely hot right after autoclaving anddrying. In this state, please do not touch it directly with your hands. It can lead to
accidents and burn injury.
Do not use the pipette for any purpose other than pipetting and liquid dispensing,such as stirring liquid with this product. It can lead to loosening of the tip, the tip
dropping off, liquid adhesion and contamination to the main unit of the device andaccidents and/or injury.
During operation and maintenance, if any worn, missing or broken parts are detected,
discontinue use immediately. Order and replace the part or parts, before use.

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2.3

Material information

CAUTION

The use of highly reactive chemicals may damage the device. Please acknowledge
the following materials that are used, and do not use liquids that will violate them.

Material of external parts
 Polypropylene (PP) Polyetherimide (PEI) modified- Polyphenyleneether (m-PPE) Polyetheretherketone (PEEK) PolyVinylidene DiFuluoride (PVDF)
 Prefluoro rubber (FFKM)
 Stainless steel Aluminum alloy Alumina－ 5－

* 3. Operation/Operating procedure
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Users of the device are required to strictly observe the following in orderfor the pipette to keep its excellent accuracy, precision and original
performance.
Do not expose pipette to direct sunlight when using it, or for 2 hours prior to use,otherwise the pipette may lose its accuracy. Avoid working with pipettes in a hightemperature, low temperature, low humidity environment, or when thetemperature difference between the environment including the main body of thedevice and the liquid is large, accuracy and precision may not be guaranteed.
This Pipette can be used in a stable environment between +4°C and +40°C, but
the specifications may vary.
Just prior to use, avoid touching the tip or nozzle cylinder as much as possible. Ifthey are warmed up, accuracy may not be obtained.
Original Nichiryo tips are recommended. Nichipet EX Plus II is calibrated with theoriginal Nichiryo tip (Premium Tip), if you use other tips, deviations in original factory settings may occur, and accuracy will not be guaranteed.
Depending on the frequency of use, the pipette should be cleaned and the airtightchamber should be maintained according to the manual.

3.1 Volume setting1) Turn the lock lever to the unlocking direction to loosen it (Fig. A).
2) Turn the push button to set the volume indicator to a desired liquid volume.
When setting the liquid volume, set the indicator’s minimum graduation scale to thetriangle point marker in the lower part of the volume indicator (Fig. B). Please refer tothe volume indicator and the minimum graduation unit for each model to set the measurement
volume accordingly. The volume indicator numbers are colored in black, or in red, toindicate the position of the decimal point. Black indicates the integer, red indicates thedecimal. For models 2uL, 10uL, 20uL, 100uL, 200uL, 1000uL scale units are in (μL),
for models 5000uL, 10000uL scale units are in (mL).
3) To increase the volume setting, turn the push button till it passes the designatedvolume setting by at least half a rotation of the push button dial, and then dial back to set
the designated volume.
4) To decrease the volume setting, simply turn it to the designated volume directly.
5) After setting the liquid volume, turn the lock lever to the locking position to lock it(Fig. A).

CAUTION

Do not exceed the specified liquid volume limit, otherwise the pipette may bedamaged or deteriorate in its quality.

－ 6－Decrease
Push button
Increase
Lock lever
Ejector button

Fig. A

Volume indicator
2μL
Model:NPLO2-2

10μL
NPLO2-10

20μL
NPLO2-20

100μL NPLO2-200
200μL NPLO2-1000
1000μL NPLO2-5000
5000μL 10000μL
NPLO2-100
NPLO2-10000

Point Mark2μL
(0.002)10μL(0.01)

20μL(0.02)

100μL
(0.1)

200μL 1000μL
(0.2)
(1)

5 mL
(10)

10 mL
(10) Minimum

graduation unit

Fig. B

3.2 Aspiration of liquid (Forward technique)1)

Attach a new tip to the nozzle end.
It is recommended that tips are directly picked up from the rack. Do not twist
the pipette when fixing tip on.
Please be sure to mount the tip on the main nozzle securely. Failure to do so
may cause the tip to drop off and liquid to splash.



CAUTION

Do not perform pipetting with less liquid than the set volume. If the quantity of liquid isless than the set volume, it may cause the liquid to spray into the main body, and thepipette may be damaged or deteriorate in its quality.
2)

Press down the push button fully to the first stop position “b”. (Fig. C) – ①
－ 7－

3)
4)

5)

Hold the pipette vertically and immerse the tip 2mm. to 3mm. below the surface ofthe liquid (Fig. D) - ①.
To aspirate the set volume of liquid into the tip, release the push button slowly andlet the push button go back to the initial position naturally. It takes 1 second (up to
3 seconds for a volume of 5000μL, 10000μL) to aspirate the liquid. During this operation, stop to wait for the suction process of the liquid to be totally completed, making sure that the liquid is drawn up into the tip with certainty (Fig. D) -②.
Draw the tip of the pipette carefully vertically upward and away from the liquidsurface, then touch the tip to the side of the tube to remove excess droplet adheringto the outside of the tip (Fig. D) - ③.

①

②

③

④

a
b
c

Ejector button

Fig. C

Do not aspirate when the push button is at ‘position ④’ (Fig. C).
We recommend using the forward technique as the operation method of thisdevice for pipetting. Nichipet EX Plus II has been calibrated with the forward
method, and precision may not be obtained when dispensing by any othermeans.
Always change the tip when using different liquids to avoid cross-contamination.

CAUTION
Please operate the push button slowly and gently.
Sudden release of the push button, can lead to the aspiration of the liquid into the nozzle
cylinder; not only precise accuracy will not be obtained, but also the quality of the device
will be impaired. Due to the larger base volume measurements of models, 1000μL,
5000μL and 10000μL, there is a higher chance of the above to occur.
To eliminate this possible situation that leads to inaccuracy, precision failure orcontamination, filters (Sold separately.) are included in the package.
We highly recommend that the ‘Filter’ is used and regularly replaced in due intervals.
(Please order and replace the filters of the respective models when needed.)When using the pipette, be sure to always attach and use a tip. Failure to do so, willlead to liquid entering the inside of the device’s nozzle cylinder and cause serious
malfunctions

Never turn the device sideways or upside down with liquid in the tip. Liquid can enter
inside the device’s nozzle cylinder, which can cause ※contamination and breakdown.
－ 8－※Cross-contamination with the entered liquid remaining inside the main body and/or nozzle

cylinder of the device can also occur, when switching liquids thereafter.

3.3 Dispensing the liquid
1) Touch the end of the tip against the inside wall of the recipient tube at a slight angle.
2) Press the push button down slowly and smoothly to the first stop “position b”.
Wait for a second, then press the push button down to the second stop “position c” toexpel the last droplet of liquid from the tip (Fig. D) - 4 and 5.
3) Keep the push button compressed. Remove the last droplet by slightly touching andrunning the end of the tip up against the inner wall of the tube, then move the tip of
the pipette vertically straight up and out of the tube.
4) Press the ejector button to detach the tip to dispose (Fig. D) – 6.
We recommend the tip to be disposed of after each use.
Repeated use may lead to and result in; loss of accuracy, precision, and maylead to contamination/cross-contamination due to adhesion and deposits fromprior use.

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a

①

②

③

b
c

Fig. D

－ 9－

④

⑤

⑥

3.4 Recommendation for accurate pipetting (Technique)
In addition to the previously mentioned operations of pipetting, the following
technique maximizes the performance of the pipette.
1) Pre-Rinsing the tip
Higher precision can be obtained by performing the pre-rinsing of the tip. When using a
fresh pipette tip for the first time and before sampling, it is important to pre-rinse the tip
at least two to three times with the target solution before pipetting. Repeat the aspirationand discharge to the position of the 1st stop consecutively for two to three times(Fig. E) – b.
When strict precision is required, this method is recommended for all kinds of liquidhandling. Pre-rinsing is a fast and easy way to increase the accuracy.
2) Handling of High Density Solutions / Viscous Solutions
After aspirating the liquid into the tip, wait for 2 to 3 seconds before removing the tip
slowly from the surface of the liquid. When dispensing, wait 2 to 3 seconds at the firststop position before pushing into the second stop position (Fig. E) – c.
3) Small volume dispensing
Especially for volumes less than 50μL, please operate the pipette slowly and smoothly.
Also, please pay close attention to the effect of evaporation loss, due to temperature andhumidity.

abcEjector button

Fig. E－ 10－

4. Maintenance
4.1 Cleaning
When Nichipet EX Plus II’s exterior is soiled, please use 70% ethanol on a clean soft cloth towipe it off.
Also, if any symptom that is described in the "7. Troubleshooting" section occurs, disassemble
and inspect the device (each part), and then check and follow the procedures in the‘Troubleshooting Table’ to identify and isolate, correct and/or fix the situation or problem.

CAUTION

Use of highly reactive liquids, may damage the device.
After using a highly reactive liquid or/and if contamination occurs, perform the disassemblyand cleaning of the device, accordingly to the following method in this manual.

In order to keep and use Nichipet EX Plus II in its best condition, we recommend
the user carries out; periodic checks and maintenance, and volume
inspection/calibration of the device.

4.2 Disassembling
1)

2)

Turn counterclockwise to loosen and remove the ejector setting screw.
(6 Models) - 2μL to 1000μL (Fig. F) - (1-1)
Turn the ejector setting screw counterclockwise to remove it, and then carefully pullout the ejector pipe, straight in the direction of the horizontal arrow.
(2 Models) - 5000μL and 10000μL (Fig. F) - (2-1)
・6 Models ranging from 2μL to 1000μL (Fig. F) - (1-2)
The nozzle cylinder (Outer threading) screws into the main body.

（１－１）⑦Ejector setting screw（２－１）⑦Ejector setting screw⑥Ejector pipe

⑥Ejector pipe

（１－２）

（２－２）

③Nozzle cylinder

Lower bodyFig. F③Nozzle cylinder

Circled Numbers in (Fig. F) corresponds to Parts Numbers in the replacement parts list.
－ 11－

・For the 2 models: 5000μL and 10000μL (Fig. F) - (2-2)
The nozzle cylinder is threaded onto the lower body. (Inner threading.)
Note the differences in design; turn counterclockwise to remove the nozzle cylinder.

3)

CAUTION
When removing the nozzle cylinder, the internal parts may spring out and apart fromthe reaction of the loaded spring inside, be mindful of this point, and take precautions
when unscrewing and taking apart the nozzle cylinder.
Disassemble the internal parts as shown in; (Fig. G1) for Models: 2μL,10μL and 20μL,(Fig. G2) for Models: 100μL and 200μL, and (Fig. G3) for the Model: 1000μL.
・For Models: 2μL and 10μL, remove the plunger, 1st spring, O-ring retainer in the correctand stated order. The O-ring and the seal ring cannot be detached. They are press-fitted
and built into the structure as an integral part of the nozzle cylinder. Please take out
only the 1st spring and the O-ring retainer.
・For Models: 20μL, 100μL, 200μL and 1000μL, remove the plunger, 1st spring, O-ringretainer, O-ring, and the seal ring in the nozzle cylinder in the correct and statedorder.
・For Models: 5000μL: (Fig. G4) and 10000μL (Fig. G5), remove the seal spring,O-ring retainer, O-ring and seal ring in the nozzle cylinder.
Please be careful not to lose any small parts during disassembly.



Depending on the Model, the specification of the O-ring retainer will be different.

4.3 Reassembling
1)

Reassembling the nozzle cylinder
・For models: 2μL, 10μL, 20μL (Fig. G1), 100μL and 200μL (Fig. G2), 1000μL (Fig. G3)
First, attach the 1st spring onto the plunger, set the O-ring retainer, seal ring andO-ring in order and correctly intact. Then, insert the assembly into the nozzle cylinderand screw it clockwise into the main body.
However, for Models: 2μL and 10μL, the O-ring and seal ring cannot be detached.
They are press-fitted and built into the structure as an integral part of the nozzle
cylinder. Please reassemble with; only the 1st spring and O-ring retainer.
・For Models: 5000μL (Fig. G4) and 10000μL (Fig. G5)
Set the volume setting below 30% of the maximum volume. (This moves the plunger
forward/out.)
Assemble together the seal ring, the O-ring and the O-ring retainer, and place theseal spring on top (Fig. H) - (1).
Place the assembly and seal spring up on a flat surface, and insert the plunger into
the seal spring until the O-ring is fully inserted.
Take extreme caution not to deform or damage the seal ring, when inserting theassembly into and onto the plunger. (Fig. H-(2)).
Put the nozzle cylinder over the plunger with the seal spring, the seal ring, the O-ringretainer and O-ring in order and correctly intact, and screw the nozzle cylinder
clockwise onto the lower body securely (Fig. H-(3)).

－ 12－


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

2)

For Models: 1000μL, 5000μL, 10000μL, securely set the seal ring and O-ring to
the O-ring retainer. If set in an uneven and unseated state, liquid leakage will
occur.
When screwing the nozzle cylinder into/onto the main body, hold the parts
vertically as a visual reference, and slowly turn to let the threads bite together
correctly. If screwed in an offset way or with force, the O-ring will be unseated,
stripping of the threads and/or damage to the casing can occur, all of which, willcause leakage.
When reassembling, be careful not to assemble the seal ring and O-ring in thewrong order or direction. Failure to do so, may cause not only air and/or liquidleakage, but also inaccuracy, failure in extracting liquid and/or contamination.

Fitting the ejector pipe to the main body.
・Models: 2μL, 10μL, 20μL (Fig G1), 100μL and 200μL (Fig. G2), 1000μL (Fig. G3)
Press the ejector button down with your thumb to help meet and align with theposition to secure, insert the ejector pipe onto the body and secure it with the ejector
setting screw. If resistance is felt when ejecting, jiggle the ejector pipe back andforth very slightly, to give it better alignment, response and action (Fig. J).
After reassembling, perform several tests to inspect the pipette’s function/reliability.

－ 13－

2μL / 10μL / 20μL

⑥O-ring retainer

Main body
Seal ring ⑤O-ringseal ringset
O-ring③NozzlecylindersetNozzle cylinder①Plunger set
Enlargement of seal ring

To nozzle cylinder⑧Ejector setting screw②1st spring⑦Ejector pipe

Fig. G1
Circled Numbers in (Fig. G1) corresponds to Parts Numbers in the replacement parts list.
－ 14－

⑥O-ring retainer

Seal ring
Main bodyO-ring⑤O-ringseal ringset③Nozzle cylinder①Plunger set

Enlargement of seal ring

To nozzle cylinder②1st
1st spring
spring②⑧Ejector setting screw⑦Ejector pipe

Fig. G2
Circled Numbers in (Fig. G2) corresponds to Parts Numbers in the replacement parts list.
－ 15－

Main body

③Nozzle cylinder①Plunger set⑨Nozzle filter②1st spring⑥O-ring retainer⑤O-ringseal ringset⑧Ejector setting
screw

Seal ring⑦Ejector pipe

O-ringEnlargement of seal ring

Fig. G3

To nozzle cylinder

Circled Numbers in (Fig. G3) corresponds to Parts Numbers in the replacement parts list.
－ 16－

Main body

③Nozzle cylinder①Plunger set

Lower
body
⑨Nozzle filter

Plunger⑧Ejector setting
screw④Seal spring⑦Ejector pipe

⑥O-ring retainer⑤O-ringseal ringset

Seal ring
Enlargement of seal ringO-ringTo nozzle cylinder

Fig. G4
Circled Numbers in (Fig. G4) corresponds to Parts Numbers in the replacement parts list.
－ 17－

Main body

③Nozzle cylinder①Plunger set

Lower
body

⑨Nozzle filter

Plunger⑧Ejector setting
screw④Seal spring⑦Ejector pipe
⑥O-ring retainer

Seal ring⑤O-ringseal ringset

Enlargement of seal ringO-ringTo nozzle cylinder

Fig. G5

Circled Numbers in (Fig. G5) corresponds to Parts Numbers in the replacement parts list.
－ 18－

（１）

（２）

Seal springO-ring retainer
Seal ringO-ringFig. H

Fig. J－ 19－（３）

4.4 Changing the Nozzle Filter
・Filter removal procedure
Model 1000μL: (Fig. K) - (1)Insert the tips of the filter remover tweezers into the two notches on the sides of the filter
and pull it out, straight in the direction of the arrow.
Model 5000 μL and 10000μL: (Fig.K) - (2)Insert the filter remover pick into the center of the filter end and pull it out, straight in the
direction of the arrow.
・Installing the filter
Insert a new filter firmly and flush, into the nozzle cylinder.

WARNING

Never directly touch filters, that may be contaminated by harmful or toxic substances.



CAUTION
The filter replacement tool tip is very sharp and can be dangerous. Please handle it withcaution.

⑩Nozzle filter remover（1）１０００μL⑨Nozzle filter（2）５０００μL ，１００００μL⑩Nozzle filter remover⑨Nozzle filter

Fig. K

Circled Numbers in (Fig. K) corresponds to Parts Numbers in the replacement parts list.

－ 20－

4.5 Autoclaving
The whole pipette is autoclavable. Carry out the autoclaving process at 121°C for 20
minutes, following the procedure below.
1) For Models: 1000μL, 5000μL and 10000μL, remove the filter.
(Refer to “Filter removal procedure” on P.20)
2) When autoclaving, always leave the lock mechanism loosened, and always check and be
sure to set the volume indicator to the ‘Maximum Volume’ of the pipette’s volume
range. (This moves the plunger back/in.)3) Start the autoclaving process. After autoclaving is done, please promptly dry out thepipette with the following procedure.

WARNING

Due to high temperature and high pressure use in autoclave sterilization, it is very
dangerous. Please operate equipment according to safety guidelines and standards.

CAUTIONWhen autoclaving, do not stack items on top of each other, do not place the nozzle
downward, or lean the device diagonally against anything, as this will apply load andstress to the heated structure.
Always autoclave the pipette with the nozzle facing upward and standing.
Although the pipette is made of autoclave compatible material, due to the hightemperature and pressure used in the sterilizer, there is a risk that heated parts
subjected to load and stress to deform.
Do not autoclave at temperatures above 121°C (There is a risk of causing damage.)－ 21－

4.6 Drying the pipette
1) As with the same when autoclaving the device, also unlock and loosen the lock leverwhen drying.
2) Remove the ejector pipe following the procedure in 4.2 Disassembling (Fig. F).
3) Turn the nozzle cylinder counterclockwise by two and a half turns to just loosen it.
4) Dry it with a blower type constant temperature dryer, and confirm that it is completely
dried out.
5) After drying, wait until the main unit has returned to room temperature, tighten thenozzle cylinder, and reattach the ejector pipe back into/onto the main body.

CAUTION

Immediately after autoclaving and drying, the pipette and parts are extremely hot, so
please do not touch them directly with your hands.
When autoclaving, there is a possibility of damage, and performance may be affected,
due to other items that are in the autoclave at the same time, and/or by substancesthat may be present in the water that is being used in the autoclave.
Assembling the pipette in a heated or warm condition after drying, can lead to damage,such as stripping of the threads. Please let it cool down first. Also, if used in a heated or
warm condition, accuracy will not be obtained.



Accuracy and precision may change with the autoclaving conditions, and alsowith many other various factors. We recommend testing the accuracy andprecision after autoclaving, and at other times when needed.

－ 22－

* 5. Specifications (Accuracy/Precision)

Table-1

Maximum Permissive Errors.

Pipette types(Code)

Volumerange

2μL(00-NPLO2-2)

0.1-2
(uL)

10μL
(00-NPLO2-10)

0.5-10(uL)

20μL(00-NPLO2-20)

2-20
(uL)

100μL
(00-NPLO2-100)

10-100(uL)

200μL(00-NPLO2-200)

20-200(uL)

1000μL(00-NPLO2-1000)

100-1000(uL)

5000μL(00-NPLO2-5000)

1000-5000(uL)

10000μL(00-NPLO2-10000)

1000-10000
(uL)

★

・
・
・
・
・

Volume(uL)

Accuracy(systematic error)
AC (%)

Precision(random error)
CV(%)

0.2
1
2
1
5
10
2
10
20
10
50
100
20
100
200
100
500
1000
1000
2500
5000
1000
5000
10000

±12.0★
±5.0
±3.0
±4.0
±1.0
±1.0
±5.0
±1.0
±1.0
±2.0
±1.0
±0.8
±1.0
±0.8
±0.8
±1.0
±0.8
±0.7
±1.0
±0.8
±0.6
±2.0
±0.8
±0.4

≤6.0★
≤2.5
≤1.0
≤3.0
≤1.0
≤0.5
≤3.0
≤1.0
≤0.4
≤1.0
≤0.3
≤0.3
≤0.5
≤0.3
≤0.2
≤0.5
≤0.3
≤0.2
≤0.3
≤0.3
≤0.2
≤0.4
≤0.3
≤0.2

Accuracy and precision of 0.2μL or less of “2μL model” are greatly affected by the user’s
sampling skills, and/or by various factors and conditions.
Tips used: Premium Tip (BMT2)
Measurement temperature: Between 20°C to 25°C
Relative humidity: above 50%
Measurement medium: distilled water
Barometric pressure, 101kPa

Volume measurement is in accordance with ISO 8655-6.

－ 23－

6. Calibration (Adjustments)
Please have adequate foreknowledge to responsibly perform all operations.
We cannot guarantee, accuracy/precision, or trouble in pipetting operation, after the user has
arbitrarily performed any adjustments after factory settings.
We thank you in advance for your understanding.
Perform the volume adjustment and volume inspection in the following method.

6.1 Volume adjustment method
To perform a good and reliable calibration for all the models of pipettes in this manual,
conduct the adjustment at minimum volume setting first, and then conduct the maximum
volume setting adjustment.
Please perform the volume check of the maximum volume after the minimum volume
accuracy falls within the standard accuracy on [Table 1].
Volume adjustment procedure
1) Loosen and unlock the lock mechanism.
2) Insert the adjustment tool’s prongs into the two grooves (Fig. L①).
3) Hold the unit in one hand and make sure the adjustment tool is fixed in (Fig. L②).
4) Rotate clockwise to increase the dispensing volume, and counterclockwise to
decrease the dispensing volume.
5) Please refer to [Table 2] for the required fine adjustment of the volume.
Clockwise:
Dispensing volume increase
Counterclockwise:
Dispensing volume decrease

Adjustment tool

Adjustment tool①②
Fig. L

Circled Numbers in (Fig. L) corresponds to Parts Numbers in the replacement parts list.

－ 24－

Table-2
2μL
10μL
20μL
100μL
200μL
1000μL
5000μL
10000μL

Calibration guideline
１ scale mark0.00150.0077
0.013
0.079
0.13
0.79
8.0
8.0

2 scale marks
0.00290.0154
0.025
0.158
0.25
1.58
16.0
16.0

5 scale marks
0.00730.0385
0.063
0.396
0.63
3.96
40.0
39.9

10 scale marks
0.01450.0769
0.126
0.791
1.26
7.92
80.0
79.9

The numerical values (volume) above are for reference only. For actual measurement, pleaseuse a balance which is properly calibrated.

6.2 Volume Measurement/Inspection Procedure* 1)

In order to avoid influence from temperature differences, ready the pipettes forinspection, the distilled water, the balance and tips 2 to 3 hours before use, in theenviron where the measurement is to be conducted to attain temperature equilibrium.
※ The measurement room should be in a controlled temperature between 20°C to
25°C, and the measurement should be held where there is no direct influence from windblowing from an air-conditioner, heater or fan.
Please use the Nichiryo Original Premium Tip Series (volume compatible ones), and be
sure to install it on the pipette from the rack.

2)

Using distilled water as the sample, aspirate and dispense the volume to be calculated
inside the weighing vessel on the balance. Please use a vessel with a lid for theweighing vessel inside the balance. Also, include a small amount of distilled water in thevessel, prior to adjusting the balance and measurement.

3)

Read the mass (mg) measured with the balance, and then convert it with the"Z correction Factor for Distilled Water[table3]" to obtain the dispensed measured volume (μL).

Vi  mi  Z
4)Vi: Volumem i : Measured massZ

: Z correction factor

Add together 1the 10 volumes delivered and divide the sum by 10 to provide the mean
volume.

V

n
1
 Vi
10 i 1－ 25－

V : Mean volume

5)

Calculate the systematic error es [%] with the equation below.

es  100 
6)V  V e s : systematic error [%]V s : selected volumes

Vs

Calculate the random error CV [%] with the equation below. Where n is the number ofmeasurements, in this case n=10 .

 V  V 
n

CV 

100

V

i 1

2

i

CV : random error [%]n 1

Table-3 Z correction factor for distilled water
Air Pressure (kPa)
Temperature
(°C)
95
100
101.3
20.0
1.00281.00281.002920.5
1.00291.00291.003021.0
1.00301.00311.003121.5
1.00311.0032
1.003222.0
1.0032
1.00331.003322.5
1.00331.00341.003423.0
1.00341.00351.003523.5
1.00361.00361.003624.0
1.00371.00371.003824.5
1.00381.00391.003925.0
1.00391.00401.0040－ 26－

105
1.00291.00301.00311.0032
1.00331.00341.00361.00371.00381.00391.00407. Troubleshooting
Symptom
Tip cannot beejected.

Pipette fails toaspirate liquid.

Possible cause

The nozzle cylinder is loose

Filter is soaked with liquid.
(1000μL , 5000μL,
10000μL)
Seal ring and O-ring areassembled in reverse
order.
Seal ring and/or O-ring set
is worn.
The nozzle cylinder is loose

Extracted
liquid leaks
from the tip.

Nozzle cylinder is worn(stepped wear can bechecked by eye).
Seal ring and O-ring set
are worn, because theplunger is damaged or
rusty.
The tip is loosely attached.

Push button
moves stiffly.

The liquid has aspirated
and leaked inside thenozzle cylinder.

RemedySecurely, tighten up thenozzle cylinder.
Replace the filter with a
new one (supplied as astandard accessory, orpurchase a filter set for
replacement).
Reset the seal ring andO-ring set according to themanual in “Disassembling/reassembling”.
Replace the seal ring andO-ring set.
Securely, tighten up thenozzle cylinder.
Replace the nozzle
cylinder.
Replace the seal ring andO-ring set.
Reattach the sameloosened tip, or with a newone firmly.
If the push button does
not work well after
dispensing, or it issticking to the body,disassemble the pipette
and wash/clean every
part (or wipe down every
part with a soft cloth).
If some parts get rusty
or corroded
inside the body, replace
the part(s) with newone(s).

When the pipette cannot be fixed after examining and conducting the above
mentioned procedure, immediately stop using the pipette and ask us or our
agent to repair it.
Before bring the pipette for repair, be sure to check whether it has been
contaminated with microbes, and/or harmful or toxic substance.
－ 27－

8. Replacement parts list
* 8.1 Consumables●Premium Tip (Bulk tip, Autoclavable)
Code

Volume range
(μL)

Color

ApplicablemodelsQ’ty

00-BMT2-UT

0.1-10

Clear

2μL

1000

00-BMT2-SSW

0.5-10

Clear

10μL

1000

00-BMT2-SG

2-200

Clear

20/100/200μL

100000-BMT2-LG

100-1000

Clear

1000μL

1000

00-BMT2-X

1000-5000

Clear

5000μL

200

Clear

10000μL

200

ApplicablemodelsQ’ty

00-BMT2-Z

1000-10000●Premium Tip (Racked tip, Autoclavable)
Code

Volume range
(μL)

Color

00-BMT2-UTWR

0.1-10Clear

2μL

960 (96pcs x 10 cases)

00-BMT2-SSWR

0.5-10

Clear

10μL

960 (96pcs x 10 cases)

00-BMT2-SGR

2-200

Clear

20/100/200μL

960 (96pcs x 10 cases)

00-BMT2-LGR

100-1000

Clear

1000μL

960 (96pcs x 10 cases)

00-BMT2-XR

1000-5000

Clear

5000μL

100 (50pcs x 2 cases)

00-BMT2-ZR

1000-10000

Clear

10000μL

80 (40pcs x 2 cases)－ 28－

8.2 Spare parts list
Content

Type in volume① 00-LO2-1000002

Plunger set 2μL

Plunger head/plunger

2μL① 00-LO2-1000010

Plunger set 10μL

Plunger head/plunger

10μL① 00-LO2-1000020

Plunger set 20μL

Plunger head/plunger

20μL① 00-LO2-1000100

Plunger set 100μL

Plunger head/plunger

100μL① 00-LO2-0100200

Plunger set 200μL

Plunger head/plunger

200μL① 00-LO2-0101000

Plunger set 1000μL

Plunger head/plunger

1000μL

Code

Description

00-LO2-0105000P
Plunger set 5000μL①
00-LO2-010500GP

Plunger /Joint block(w/O-ring) /Joint shaft / Joint plate /Plunger head / Setting
screw / First spring /Lower body
Plunger /Joint block(w/O-ring) /joint shaft / Joint plate /Plunger head / Setting
screw / First spring /Lower body

5000μL① 00-LO2-0110000P

Plunger set 10000μL

10000μL② 00-LO2-2000002

First spring 2μL------

2μL② 00-LO2-2000010

First spring 10μL------

10μL② 00-LO2-2000020

First spring 20μL------

20μL② 00-LO2-2000100

First spring 100μL------

100μL② 00-LO2-2000200

First spring 200μL------

200μL② 00-LO2-2001000

First spring 1000μL------

1000μL－ 00-LO2-2005000

First spring 5000μL------

5000μL－ 00-LO2-2010000

First spring 10000μL------

10000μL③ 00-LO2-4000002

Nozzle cylinder set 2μL

Nozzle cylinder/K bush

2μL

③ 00-LO2-4000010

Nozzle cylinder set 10μL

Nozzle cylinder/K bush

10μL

③ 00-LO2-0400020

Nozzle cylinder set 20μL

Nozzle cylinder/K bush

20μL

③ 00-LO2-0400100

Nozzle cylinder 100μL------

100μL③ 00-LO2-0400200

Nozzle cylinder 200μL------

200μL③ 00-LO2-0401000

Nozzle cylinder 1000μL------

1000μL

Nozzle cylinder 5000μL------

5000μL00-LO2-0405000
00-LO2-0405000G
00-LO2-0410000③
00-LO2-0410000G④ 00-LO2-2205000

Nozzle cylinder 10000μL-------

10000μL

Seal spring 5000μL------

5000μL④ 00-LO2-2210000

Seal spring 10000μL-------

10000μL

③

－ 29－

Code

Description

Content

Type in volume

O-ring/seal ring

2μL

O-ring/seal ring

10μL

O-ring/seal ring

20μL

O-ring/seal ring

100μL

O-ring/seal ring

200μL

O-ring/seal ring

1000μL

O-ring/seal ring

5000μL⑤ 00-LO2-0090001

O-ring seal ring set
2μL
O-ring seal ring set
10μL
O-ring seal ring set
20μL
O-ring seal ring set
100μL
O-ring seal ring set
200μL
O-ring seal ring set
1000μL
O-ring seal ring set
5000μL
O-ring seal ring set
10000μL
O-ring retainer⑤ 00-LO2-0090002

O-ring retainer------⑤ 00-LO2-0090003

O-ring retainer------

20μL⑤ 00-LO2-0090004

O-ring retainer------

100μL⑤ 00-LO2-0090005

O-ring retainer------

200μL⑤ 00-LO2-0090006

O-ring retainer------

1000μL⑤ 00-LO2-0090007

O-ring retainer------

5000μL⑥ 00-LO2-0900010

Ejector pipe A------

2/10μL⑥ 00-LO2-0900200

Ejector pipe B------

20/100/200μL⑥ 00-LO2-0901000

Ejector pipe C------

1000μL⑥ 00-LO2-0905000

Ejector pipe D------

5000μL⑥ 00-LO2-0910000

Ejector pipe E⑤ 00-LO2-3000002⑤ 00-LO2-3000010⑤ 00-LO2-3000020⑤ 00-LO2-3000100⑤ 00-LO2-3000200⑤ 00-LO2-3001000
00-LO2-3005000⑤
00-LO2-3005000G⑤ 00-LO2-3010000N

O-ring/seal ring/O-ring retainer------

10000μL
2μL
10μL------

100000μL

Setting screw/Spring
washer/Washer

2/10/20/100/
200/1000μL⑦ 00-LO2-0801000

Ejector setting screw set⑦ 00-LO2-0815000

Ejector setting screw------

5000/10000μL⑧ 00-NP-0601000

Nozzle filter (10pcs)------

1000μL⑧ 00-NX2-0615000

Nozzle filter (100pcs)------

5000/10000μL⑨ 00-NX2-1100000

Nozzle filter remover------

1000μL⑨ 00-NX2-1500000

Nozzle filter remover------

5000/10000μL⑩ 00-LO2-1600000

Volume adjustment tool------All

Please note that the specifications of the accessories may be changed without notice.
Always check our website for the latest specifications and information.
※Copying/Reprinting the manual in whole or in part without permission, is prohibited by law.
－ 30－

Inspection and Calibration Statement
The enclosed pipette was tested and calibrated under closely controlled environmental conditions
to ensure that it meets published calibration specifications. The precision and accuracy results
obtained for this pipette are provided on the enclosed calibration certificate. Because temperatureand humidity conditions affect the calibration results of liquid measurement devices, yourpipette should be calibrated under conditions of use. The calibration results obtained in yourlaboratory may vary from our results, due to differences in the environmental testing conditions.

Information on repair or servicing, when contacting your local distributor.

Authorized Representative：
Emergo Europe B.V.
Prinsessegracht 202514 AP, The Hague The Netherlands

Manufacturer：

Head office & Factory
2760-1 Nishikata, Koshigaya-Shi, Saitama 343-0822, Japan
TEL: +81-48-989-1301 FAX: +81-3-6273-7944
Tokyo office (Sales Division)
PMO Kandaiwamoto-cho Ⅱ 4F, 2-2-4 Iwamoto-cho Chiyoda-ku,
Tokyo 101-0032 Japan
TEL: +81-3- 5829-4539 FAX: +81-3- 5829-4504
URL: http://www.nichiryo.co.jp/
E-mail: i-sales@nichiryo.co.jp