User’s Manual

*SB-NAR002ER 2022/6/10
SB-NAR001ER 2022/4/15

Thank you very much for purchasing Nichipet Air.
Please read this manual carefully before using this device.
Safety Precautions








Please read this manual carefully and have sufficient understanding of thecontents and instructions, especially concerning matters of safety, prior touse.
The notes stated here is for the safety of the user, and for the correct
usage of the product.
Contents marked with
“Danger Level Symbols” are matters that
require the user’s utmost attention, not only for using Nichipet Air properly,
but also to prevent users from injuries or death, harm to others, and/or
property damage.
After reading this manual, please keep it in a noticeable and accessible
place for ‘users of the device’ to refer to at any time.
Any serious incidents that has occurred in relation to the equipment shallbe reported to the manufacturer and to the competent authority of theMember State in which the user and/or the patient is established.

Danger Levels
DANGER

Will lead to serious injuries or death.

WARNINGMay lead to severe injuries or death.

CAUTION

May lead to light to moderate injuries, and/or cause
property damage.
User information

Caution on disposal of this product
When disposing the pipette body and tips (including adhering liquid), please
comply with the laws and regulations of each country related to disposal, or local
ordinance or regulation.
- 1 -Table of Contents
1. Product overview
1.1 Features
1.2 Standard accessories (Included)
1.3 Main illustration

3
3
3
4

2. Information on safety
2.1 Intended use
2.2 Material information

56
6

3. Operation/Operating procedure
3.1 Volume setting
3.2 Aspiration of liquid (Forward technique)
3.3 Dispensing the liquid
3.4 Recommendation for accurate pipetting (Technique)

6
6
7
8
9

4. Maintenance
4.1 Cleaning
4.1.1 Disassembling
4.1.2 Reassembling
4.1.3 Lubrication/Greasing
4.2 Autoclaving
4.2.1 Autoclave implementation procedure
4.2.2 Drying procedure

10
10
10
14
21
31
31
31

5. Specifications (Accuracy/Precision)33

6. Calibration (Adjustments)
6.1 Volume adjustment method
6.2 Volume measurement/Inspection procedure

35
35
36

7. Troubleshooting38

8. Replacement parts list
8.1 Consumables
8.2 Spare parts list

39
39
39

- 2 -

1. Product overview
1.1 Features








The newly designed curvature and roundness in shape is ergonomic, and mitigates
the user’s fatigue and stress levels from long periods of use.
Fully autoclavable at the condition of 121°C for 20 minutes.
Eight types of single channel can be used for a wide range of sampling, from 0.1µL to
10000µL. Four types of volume range for 8 and 12 channels can be used for a wide
range of sampling, from 0.5µL to 300µL.In addition, the volume sizes are indicated bycolors, 2μL-gray, 10μL-pink, 20μL-cream, 100μL-orange, 200μL-yellow, 300µLwhite ,1000μL-blue, 5000μL-green, 10000μL-white.
Since each type is displayed in a different color, they are easily identifiable.
The body's hermetic part/airtight part uses high-performance and high-heatresistant grease, thus maintaining excellent accuracy and precision, even whenused over long periods of time.
The special design and structure, makes it easy and intuitive to calibrate.

1.2 Standard accessories (Included)(1)Single channel
Accessories
Tip (2µL, 10µL, 20µL, 100µL, 200µL, 1000µL,)

QTY
3

Tip (5000µL,10000µL)

1

Grease1
31Nozzle filter (5000µL,10000µL)
User’s Manual(2)Multi channel
Accessories
Tip

QTY
12

Grease1
1User’s Manual

When unboxing the package, check that all of the items above are included for therespective model.

- 3 -1.3 Main illustration(1)Single channel
Push button
Volume adjuster
Ejector button
Volume indicator
Main body

5000・10000μL
Ejector pipe

Ejector pipe(2)Multi channel
Push button
Volume adjuster
Ejector button

Main body

Ejector unit

Bottom unit

- 4 -

2. Information on safety
2.1

Intended use
Nichipet Air, used in conjunction with pipette tips recommended by Nichiryo, aredesigned and constructed for low-contamination transfer of liquids, especially forsamples from the human body and for reagents within the scope of an in-vitro
diagnostic application in order to allow the in-vitro diagnostic medical device to be
used as intended.
Therefore, Nichipet Air is subjected to the accessories of in-vitro diagnostic medical
devices under Regulation (EU) 2017/746. The accessories is treated as in vitro diagnostic
medical devices in their own right under Regulation (EU) 2017/746.
Nichipet Air are intended for operation by qualified staff.

DANGER
When handling radioactive substances or infectious substances, always check andconfirm the information first, and follow guidelines on their safety procedures.
When using harmful liquids to the human body, be very careful in the handling of thesubstances.

Never touch or come into direct contact with the used chips.
When liquids that are harmful to the human body adheres and/or contamination occurs,
use appropriate measures to clean and decontaminate the device before continuing
its use.
This product is not intended for use on living organisms.

WARNINGDo not eject the tip with liquid inside of it.
Do not eject the tip towards anybody.
Do not expel or dispense any liquids towards anybody.
Depending on the splashed liquid, there is danger of causing injuries to the humanbody.
Please protect yourself in accordance with the general procedure of danger prevention,such as wearing protective clothing, protective glasses and gloves.

CAUTIONDo not use the pipette for any other purpose, use only for pipetting and liquid dispensing.
Do not modify the pipette, modification can lead to accidents.
Do not stab the tip into the human body, or eject the tip towards anyone. The tip isvery sharp and extremely dangerous.
Since the main body of the pipette becomes extremely hot right after autoclaving anddrying. In this state, please do not touch it directly with your hands. It can lead to accidents
and burn injury.
Do not use the pipette for any purpose other than pipetting and liquid dispensing,such as stirring liquid with this product. It can lead to loosening of the tip, the tip
dropping off, liquid adhesion and contamination to the main unit of the device andaccidents and/or injury.

- 5 -

2.2

Material information
CAUTION
The use of highly reactive chemicals may damage the device. Please acknowledge
the following materials that are used, and do not use liquids that will violate them.



Material of external parts
 Polypropylene (PP) modified- Polyphenyleneether (m-PPE) Polyetheretherketone (PEEK) Fluororubber (FKM) Stainless steel Aluminum alloy
 Polysulfone (PSU) Polyphenylenesulfide (PPS) Polyetherimide (PEI)* 3. Operation/Operating procedureDo not expose pipette to direct sunlight when using it, or for 2 hours prior to use,otherwise the pipette may lose its accuracy. Avoid working with pipettes in a hightemperature, low temperature, low humidity environment, or when thetemperature difference between the environment including the main body of thedevice and the liquid is large, accuracy and precision may not be guaranteed.
Just prior to use, avoid touching the tip or nozzle cylinder as much as possible. Ifthey are warmed up, accuracy may not be obtained.
Original Nichiryo tips are recommended. Nichipet Air is calibrated with theoriginal Nichiryo tip(Premium Tip), if you use other tips, deviations in original
factory settings may occur, and accuracy will not be guaranteed.

3.1 Volume setting1) Turn the volume setting rotator to set the volume indicator to a desired liquidvolume. When setting the liquid volume, set the indicator’s minimum graduation
scale to the triangle point marker in the lower part of the volume indicator (Fig.3-1).
Please refer to the volume indicator and the minimum graduation unit for each model
to set the measurement volume accordingly. The volume indicator numbers arecolored in black, or in red, to indicate the position of the decimal point. Black indicatesthe integer, red indicates the decimal. Scale units are in (μL).
2) To increase the volume setting, turn the volume setting rotator till it passes thedesignated volume setting by at least half a rotation of the rotator, and then dial backto set the designated volume.
3) To decrease the volume setting, simply turn it to the designated volume directly.
(Fig. 3-1)

- 6 -

CAUTION
Do not exceed the specified liquid volume limit, otherwise the pipette may bedamaged or deteriorate in its quality.



Volume indicator
Model: 2µL

10µL

20µL

100µL

200µL

300µL

1000µL

5000µL

10000µL

Point
mark(0.002)(0.01)(0.02)

(0.1)

(0.2)

(1)

(1)

(5)

( 10 )

Minimum scale [µL]

Fig. 3-1

3.2 Aspiration of liquid (Forward technique)1)

Attach a new tip to the nozzle end.



It is recommended that tips are directly picked up from the rack. Do not twist
the pipette when fixing tip on.
Please be sure to mount the tip on the main nozzle securely. Failure to do so
may cause the tip to drop off and liquid to splash.

CAUTION

Do not perform pipetting with less liquid than the set volume. If the quantity of liquid isless than the set volume, it may cause the liquid to spray into the main body, and thepipette may be damaged or deteriorate in its quality.
2)
3)

Press down the push button fully to the ‘1st stop’.
Hold the pipette vertically and immerse the tip 2mm. to 3mm. below the surface ofthe liquid (Fig. 3-2) - ①.
4) To aspirate the set volume of liquid into the tip, release the push button slowly andlet the push button go back to the initial position naturally. It takes 1 second toaspirate the liquid. During this operation, stop to wait for the suction process of theliquid to be totally completed, making sure that the liquid is drawn up into the tip
with certainty (Fig. 3-2) -②.
5) Draw the tip of the pipette carefully vertically upward and away from the liquidsurface, then touch the tip to the side of the tube to remove excess droplet adheringto the outside of the tip (Fig. 3-2) - ③.

- 7 -






Do not aspirate when the push button is at ‘2nd position’ (Fig. 3-2).
We recommend using the forward technique as the operation method of thisdevice for pipetting. Nichipet Air has been calibrated with the forward method,and precision may not be obtained when dispensing by any other means.
Always change the tip when using different liquids to avoid cross-contamination.

CAUTION
Please operate the push button slowly and gently.
Sudden release of the push button, can lead to the aspiration of the liquid into the nozzle
cylinder; not only precise accuracy will not be obtained, but also the quality of the device
will be impaired.
When using the pipette, be sure to always attach and use a tip. Failure to do so, willlead to liquid entering the inside of the device’s nozzle cylinder and cause serious
malfunctions

Never turn the device sideways or upside down with liquid in the tip. Liquid can enter
inside the device’s nozzle cylinder, which can cause ※contamination and breakdown.
※Cross-contamination with the entered liquid remaining inside the main body of the device

can also occur, when switching liquids thereafter.

3.3 Dispensing the liquid
1) Touch the end of the tip against the inside wall of the recipient tube at a slight angle.
2) Press the push button down slowly and smoothly to the ‘1st stop’.
Wait for a second, then press the push button down to the ‘2nd stop’ to expel the last
droplet of liquid from the tip (Fig. 3-2) - ④ and ⑤.
3) Keep the push button compressed. Remove the last droplet by slightly touching andrunning the end of the tip up against the inner wall of the tube, then move the tip of
the pipette vertically straight up and out of the tube.
4) Press the ejector button to detach the tip to dispose (Fig. 3-2) – ⑥.


We recommend the tip to be disposed of after each use.
Repeated use may lead to and result in; loss of accuracy, precision, and maylead to contamination/cross-contamination due to adhesion and deposits fromprior use.

- 8 -

Original
position

①

②

④

③

⑤

⑥

Fig. 3-2

3.4 Recommendation for accurate pipetting (Technique)
1) Pre-Rinsing the tip
Higher precision can be obtained by performing the pre-rinsing of the tip. When using a
fresh pipette tip for the first time and before sampling, it is important to pre-rinse the tip
at least two to three times with the target solution before pipetting. Repeat the aspirationand discharge to the position of the 1st stop consecutively for two to three times(Fig. 3-3).
When strict precision is required, this method is recommended for all kinds of liquidhandling. Pre-rinsing is a fast and easy way to increase the accuracy.
Original position

1st stop
2nd stop

Fig. 3-3- 9 -2) Handling of Viscous Solutions
After aspirating the liquid into the tip, wait for more seconds than normal viscosity before
removing the tip slowly from the surface of the liquid. When dispensing, wait 2 to 3
seconds at the first stop position before pushing into the second stop position (Fig. 3-3).
3) Small volume dispensing
Especially for volumes less than 50μL, please operate the pipette slowly and smoothly.
Also, please pay close attention to the effect of evaporation loss, due to temperature andhumidity.

4. Maintenance
4.1 Cleaning
When Nichipet Air’s exterior is soiled, please use 70% ethanol on a clean soft cloth to wipe it
off.
Also, if any symptom that is described in the "7. Troubleshooting" section occurs, disassemble
and inspect the device (each part), and then check and follow the procedures in the‘Troubleshooting Table’ to identify and isolate, correct and/or fix the situation or problem.

CAUTIONUse of highly reactive liquids, may damage the device.
After using a highly reactive liquid or/and if contamination occurs, perform the disassemblyand cleaning of the device, accordingly to the following method in this manual.

✓In order to keep and use Nichipet AIR in its best condition, we recommend the user
carries out; periodic checks and maintenance, and volume inspection/calibration of
the device.

4.1.1 Disassembling(1)Single channel
1) Turn the ejector pipe counterclockwise until it stops, and pull it out. (Fig. 4-1)
2) Turn the nozzle securing nut in the direction of the arrow to remove the nozzle cylinder.
(Fig. 4-1)

- 10 -

Nozzlesecuring nut
Nozzlesecuring nut

NozzlecylinderNozzle cylinder

Fig. 4-1

CAUTION

When removing the nozzle cylinder, disassemble it carefully, as the internal parts may
pop out due to the action of the spring.

3) Take out each part as needed and accordingly.
・2 μL, 10 μL (Fig. 4-7, 4-8)
The hermetic part/airtight part is integrated with the nozzle cylinder.
(It cannot be disassembled furthermore.)
・20μL, 100μL, 200μL, 1000μL (Fig. 4-9 to 4-12)
Take out the seal spring, O-ring retainer and O-ring.

✓

The shape of the O-ring retainer varies, depending on the volume type.
Please be careful and refer to the figures for details.

- 11 -

(2)Multi channel
1) Rotate the bottom unit with holding the ejector securing screw, and remove the ejector
unit from the ejector securing screw (Fig. 4-2).
2) Split the screw part of the ejector (Fig. 4-2).
3) Pull out the ejector unit from the bottom unit. (Fig. 4-2)

2)
Ejector securing screw
Bottom unit

2)

1)

1)

Ejector unit

3)

Fig. 4-2

4) With holding the casing nut, rotate the bottom unit to be removed completely, and pullout it from the casing nut. (Fig. 4-3)

Casing nut

4)Fig. 4-3

4)

5)- 12 -

5) With pressing the spring retainer a little, turn by 90 degrees and adjust the nose shape ofplunger fixing unit and the hole shape of spring retainer to be removed the spring retainer
and the first spring.(Fig. 4-4)
Spring retainerFirst spring
Plunger fixing unit
Bottom unit

Top view of spring retainer(Dot pattern : spring retainer)

Fig. 4-4
6) Unscrew the four corners to remove the upper case. (Fig. 4-5)

Upper case

Lower caseFig. 4-57) Lift up the tip cone to be removed it from the lower case (Fig. 4-6)

Tip coneFig. 4-6- 13 -

8) Pull out the plunger from the nozzle cylinder, and remove the J spring from thenozzle cylinder. (Fig. 4-7)

Plunger
J spring

Nozzle cylinder

Fig. 4-7
CAUTION

Be careful not to lose the J spring.
Do not perform the further dismantle of the nozzle cylinder unit. Otherwise,
airtightness might be deteriorated.

4.1.2 Reassembling(1)Single channel
1) Installation of the nozzle cylinder.
・ 2 μL, 10 μL (Fig. 4-8, 4-9)
Insert the plunger into the nozzle cylinder, and turn the nozzle securing nut clockwise,
and tighten.
・ 20μL, 100μL, 200μL, 1000μL (Fig. 4-10 to 4-13)
Attach the seal spring, O-ring retainer, and O-ring to the plunger, insert the plunger
into the nozzle cylinder, and turn the nozzle securing nut clockwise, and tighten.
・ 5000μL, 10000μL (Fig. 4-14, 4-15)
After replacing the piston lip if necessary, insert the piston into the nozzle cylinder,
and turn the nozzle securing nut clockwise, and tighten.

When assembling, be careful not to make any mistakes in the order and themounting orientation of the O-ring retainer and O-ring. If installed in an
opposite way (upside down), airtightness cannot be maintained, causing
malfunctions, such as, water leaks, accuracy errors, or liquids not being
aspirated.
When replacing the piston lip, be careful not to damage the piston lip.
Also, pay attention to the correct mounting orientation. (Right-side up.)

- 14 -

2μL

Nozzle cylinder set 2µL

PlungerEjector pipe 2µL/10µL

Fig. 4-8- 15 -

10μL

Nozzle cylinder set 10µL

PlungerEjector pipe 2µL/10µL

Fig. 4-9- 16 -

20μL

Seal springO-ring retainer 20µL

O-ring 20µL

Plunger
Nozzle cylinder set 20µL

Ejector pipe 20µL/100µL/200µL

Fig. 4-10- 17 -

100μL

Seal springO-ring retainer 100µL※Pay attention to theorientation

O-ring 100µL

Nozzle cylinder set 100µL

PlungerEjector pipe 20µL/100µL/200µL

Fig. 4-11- 18 -

200μL

Seal springO-ring retainer 200µL※Pay attention to theorientation

O-ring 200µL

Nozzle cylinder set 200µL

PlungerEjector pipe 20µL/100µL/200µL

Fig. 4-12- 19 -

1000μL

Seal springO-ring retainer 1000µL※Pay attention to theorientation

O-ring 1000µL

Nozzle cylinder set 1000µL

PlungerEjector pipe 1000µL

Fig. 4-13- 20 -

5000μL

Piston lip 5000µL※Pay attention to theorientation

PistonNozzle cylinder set 5000µL

Ejector pipe 5000µL

Fig. 4-14- 21 -

10000μL

Piston lip 10000µL※Pay attention to theorientation

PistonNozzle cylinder set 10000µL

Ejector pipe 10000µL

Fig. 4-15- 22 -

2) Attach the ejector pipe so that the alignment marks are aligned, and turn it in the directionof the arrow until it clicks. (Fig. 4-16)
After assembling, perform the break-in operation several times to check that there is noabnormality in operation before use.
3) For the 5000 μL and 10000 μL types, the attached nozzle filter can be attached to thenozzle as required. Insert the nozzle filter firmly into the nozzle before use. (Fig. 4-17)

CAUTION

Never directly touch nozzle filters that are contaminated with liquids that areharmful to the human body.

Alignment markAlignment markFig. 4-16

nozzle Filter

Fig. 4-17- 23 -

(2)Multi channel
1) Insert the J spring to the nozzle cylinder, and the plunger is inserted as well.
Install the nozzle cylinder units to the channels in reverse order.

Lower caseNozzle cylinder unit

Fig. 4-18- 24 -

１０μL

Plunger

J spring

NozzlecylinderFig. 4-19- 25 -

１００μL

Plunger

J spring

NozzlecylinderFig. 4-20- 26 -

２００μL

Plunger

J spring

NozzlecylinderFig. 4-21- 27 -

３００μL

Plunger

J spring

NozzlecylinderFig. 4-22- 28 -

2) Set the upper case to the lower case, and fix the four screws on the corners.
3) The first spring is compressed a little, and inserted to the plunger fixing unit withmatching the groove of spring retainer and the shape of upper side of the rod, androtate the spring retainer by 90 degrees to be fixed.
4) With holding the casing nut, the bottom unit is rotated and inserted to be fixed.
5) With splitting the screw side of ejector, attached to the bottom unit and fix it with theejector securing screw.
When installing the ejector unit, pay attention to the correct mounting
orientation. The orientation of four corner screws of the bottom unit and twoscrews of the ejector unit should be same to be reassembled.



4.1.3 Lubrication/Greasing
Grease application(1)Single channel
・ 2μL ~ 1000μL
Depress the push button to expose the plunger, and apply a thin layer of the supplied
grease to it. (Fig. 4-23) Shaded in gray.
・ 5000μL, 10000μL (Fig.4-23)
Apply the supplied grease thinly to the specified location onto the inner surface of thenozzle cylinder. (Fig.4-23) Shaded in gray.

(2)Multi channel
・ 10μL, 100μL
With remove the plunger and apply the supplied grease thinly to the specific location.
(Fig.4-24)
・ 200μL, 300μL
Apply the supplied grease thinly to the specified location onto the inner surface of thenozzle cylinder. (Fig.4-24) Shaded in gray.

CAUTION

Be sure to only use the specified grease. Use of any other grease will void thewarranty.

- 29 -Plunger(Apply grease)

Inner surface of thenozzle cylinder（Apply grease）

Fig. 4-23

Inner surface of thenozzle cylinder（Apply grease）

Plunger(Apply grease)

Fig. 4-24- 30 -

4.2 Autoclaving
The whole pipette is autoclavable. Carry out the autoclaving process at 121°C for 20
minutes, following the procedure below.

4.2.1 Autoclave implementation procedure
1) For Models: 5000μL and 10000μL, remove the nozzle filter.
2) When autoclaving, always check and be sure to set the volume indicator to the‘Maximum Volume’ of the pipette’s volume range. (This moves the plunger back/in.)3) Start the autoclaving process. After autoclaving is done, please promptly dry out thepipette with the following procedure.







WARNING
Due to high temperature and high pressure use in autoclave sterilization, it is very
dangerous. Please operate equipment according to safety guidelines and standards.

CAUTION
When autoclaving, do not stack items on top of each other, do not place the nozzle
downward, or lean the device diagonally against anything, as this will apply load andstress to the heated structure.
Always autoclave the pipette with the nozzle facing upward and standing.
Although the pipette is made of autoclave compatible material, due to the hightemperature and pressure used in the sterilizer, there is a risk that heated parts
subjected to load and stress to deform.
Do not autoclave at temperatures above 121°C (There is a risk of causing damage.)

4.2.2 Drying procedure(1)Single channel
1)
2)
3)
4)

Remove the ejector pipe according to ‘4.1.1 Disassembling’.
Loosen the nozzle securing nut by making a half turn counterclockwise.
Put the disassembled parts into a blower type constant temperature dryer to beheated. Confirm these parts are completely dried out after heating.
After drying, confirm these parts have returned to room temperature, turn thenozzle securing nut clockwise to be fixed into the nozzle cylinder, and reattach theejector pipe.

(2)Multi channel
1)
2)
3)
4)

Remove the ejector unit according to ‘4.1.1 Disassembling’.
With holding the casing nut, loosen the bottom unit by rotating 360 degrees
counterclockwise.
Put the disassembled parts into a blower type constant temperature dryer to beheated. Confirm these parts are completely dried out after heating.
After drying, confirm these parts have returned to room temperature, tighten thecasing nut to fix the bottom unit, and assemble the ejector unit according to ‘4.1.2
Reassembling’.

- 31 -

CAUTION
Immediately after autoclaving and drying, the pipette and parts are extremely hot, so
please do not touch them directly with your hands.
When autoclaving, there is a possibility of damage, and performance may be affected,
due to other items that are in the autoclave at the same time, and/or by substancesthat may be present in the water that is being used in the autoclave.
Assembling the pipette in a heated or warm condition after drying, can lead to damage,such as stripping of the threads. Please let it cool down first. Also, if used in a heated or
warm condition, accuracy will not be obtained.



Accuracy and precision may change with the autoclaving conditions, and alsowith many other various factors. We recommend testing the accuracy andprecision after autoclaving, and at other times when needed.

- 32 -

* 5. Specifications (Accuracy/Precision)(1)Single channel

Table-1

Maximum Permissive Errors.

Models（Code）

volume range

2µL（00-NAR-2）

0.1-2
（µL）

10µL
（00-NAR-10）
20µL（00-NAR-20）
100µL（00-NAR-100）
200µL（00-NAR-200）
1000µL（00-NAR-1000）
5000µL（00-NAR-5000）
10000µL（00-NAR-10000）★

0.5-10
（µL）
2-20
（µL）
10-100（µL）
20-200（µL）
100-1000（µL）
500-5000（µL）
1000-10000（µL）

Volume(µL)

Accuracy(systematic error)
AC (%)

Precision(random error)
CV (%)

0.2±12.0★

≦6.0★

1

±2.5

≦1.5

2

±1.4

≦0.7

1

±2.5

≦1.8

5

±1.5

≦0.8

10

±1.0

≦0.4

2

±3.0

≦1.5

10

±1.2

≦0.6

20

±1.0

≦0.3

10

±3.0

≦1.0

50

±1.0

≦0.3

100

±0.8

≦0.2

20

±1.8

≦0.7

100

±1.0

≦0.3

200

±0.6

≦0.2

100

±1.0

≦0.6

500

±0.8

≦0.2

1000

±0.6

≦0.2

500

±2.0

≦0.6

2500

±0.7

≦0.25

5000

±0.5

≦0.15

1000

±2.0

≦0.6

5000

±0.8

≦0.2

10000

±0.5

≦0.15

Accuracy and precision of 0.2μL or less ofuser’s sampling skills.

“2µL model” are greatly affected by the- 33 -

(2)Multi channel

Table-2

Maximum Permissive Errors.

Models（Code）

volume range

10µL（00-NAR-10M8）（00-NAR-10M12）

0.5-10（µL）

100µL（00-NAR-100M8）（00-NAR-100M12）
200µL（00-NAR-200M8）（00-NAR-200M12）
300µL（00-NAR-300M8）（00-NAR-300M12）

・
・
・
・
・

10-100（µL）
20-200（µL）
30-300（µL）

Volume(µL)

Accuracy(systematic error)
AC (%)

Precision(random error)
CV (%)

1

±8.0

≦5.0

5

±4.0

≦2.0

10

±2.0

≦1.0

10

±3.0

≦2.0

50

±1.0

≦0.8

100

±0.8

≦0.3

20

±3.0

≦1.5

100

±1.0

≦0.7

200

±0.8

≦0.3

30

±3.0

≦1.0

150

±1.0

≦0.5

300

±0.8

≦0.3

Tips used: Premium Tip (BMT2)
Measurement temperature: Between 20°C to 25°C
Relative humidity: above 50%
Measurement medium: distilled water
Barometric pressure, 101kPa
Volume measurement is in accordance with ISO 8655-6.

- 34 -

6. Calibration (Adjustments)
Perform the volume adjustment, and volume inspection, by following the procedures
detailed below.
If you want to adjust the volume yourself, we recommend that you record and manage
the details of the adjustment.
Please note that when the customer adjusts the volume, the accuracy and precision of thevolume, and subsequent malfunctions will not be covered by the warranty.
✓

The volume adjustment jig used for adjustments is not included with this product.
If necessary, please contact us.

6.1 Volume adjustment method
First, adjust the volume with the minimum volume for each model.
E.g. (100 μL for the 1000 μL model).
Confirm that the volume accuracy of the minimum volume is within the numericalvalue compared to the standard accuracy table. (Table-1)
Then proceed in order, to check the volume accuracy of the maximum volume.

<Procedure>
1) Measure the volume adjustment value in advance (The actual volume dispensed isthe volume that is to be used for volume adjustment.) by the volume inspection
method described in the next section. It is recommended to use the average valueof multiple measurements in consideration of measurement deviation.
(Example) The following describes an example in which the volume setting of thepipette is 100 μL, and the actual measured dispensing volume is “99 μL”.
2) Turn the push button cover in the direction of the arrow and remove it. (Fig. 6-1)
3) Remove the button locking screw with a screwdriver, and remove the push button
sleeve. (Fig. 6-1)※

Push button cover

Button locking screw

Push button sleeveFig. 6-1
4) Set the adjustment tool projection so that it fits into the adjustment screw recess.
(Fig. 6-2)
5) Hold the pipette in one hand and hold down the adjustment tool firmly so that it does
not rotate. (Fig. 6-2)
6) Rotate the volume setting rotator and adjust the volume indicator value to the volume
Adjustment value “99μL”.

- 35 -

Adjustment tool
Adjustment screw

Rotate the volume
setting rotator

Fig. 6-2
7) Pay attention to the mating surfaces and orientation, attach the push button sleeveto the shaft in the reverse order of removal, and tighten it with the button locking
screw. (Fig. 6-3)
8) Align the tab on the push button cover with the D-cut on the push button sleeve, andsecure it by turning it in the direction of the arrow. (Fig. 6-3)
Push button sleeve

Tab on the pushbutton cover
Align the shape

Shaft

Button locking screw

D-cut on the pushbutton sleeveFig. 6-3

6.2 Volume measurement/Inspection procedure* 1)

In order to avoid influence from temperature differences, ready the pipettes forinspection, the distilled water, the balance and tips 2 to 3 hours before use, in theenviron where the measurement is to be conducted to attain temperature equilibrium.
※ The measurement room should be in a controlled temperature between 20°C to 25°C,
and the measurement should be held where there is no direct influence from windblowing from an air-conditioner, heater or fan.
Please use the Nichiryo Original Premium Tip Series (volume compatible ones), and be
sure to install it on the pipette from the rack.

2)

Using distilled water as the sample, aspirate and dispense the volume to be calculated
inside the weighing vessel on the balance. Please use a vessel with a lid for theweighing vessel inside the balance. Also, include a small amount of distilled water in thevessel, prior to adjusting the balance and measurement.

- 36 -

3)

Read the mass (mg) measured with the balance, and then convert it with the"Z correction Factor for Distilled Water[table3]" to obtain the dispensed measured volume (μL).
Vi

Vi  mi  Z
4)

ZV : Mean volume

Calculate the systematic error es [%] with the equation below.

es  100 6): Z correction factor

Add together the 10 volumes delivered and divide the sum by 10 to provide the mean
volume.

n1V   Vi
10 i 1

5): Volumem i : Measured massV  V e s : systematic error [%]V s : selected volumes

Vs

Calculate the random error CV [%] with the equation below. Where n is the number ofmeasurements, in this casen=10 .

 V  V nCV Table-3

100

V

2

i 1

i

CV : random error [%]n 1

Z correction factor for distilled water(Mass / Volume Conversion Factor)

Air Pressure (kPa)

Temperature
(°C)

95.0

100.0

101.3

105.0

20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
24.0
24.5
25.0

1.00281.00291.00301.00311.0032
1.00331.00341.00361.00371.00381.00391.00281.00291.00311.0032
1.00331.00341.00351.00361.00371.00391.00401.00291.00301.00311.0032
1.00331.00341.00351.00361.00381.00391.00401.00291.00301.00311.0032
1.00331.00341.00361.00371.00381.00391.0040- 37 -

7. Troubleshooting
Symptom
Tip cannot be ejected.

Pipette fails toaspirate liquid.

Extracted liquid leaks fromthe tip.

Possible cause

RemedyThe nozzle securing nut is loose

Securely, tighten up the nozzle cylinder.

The ejector pipe is loose

Replace the ejector pipe correctly.

Nozzle tip is clogged (Foreign
matter adhered)

Clean or replace nozzle cylinder parts

The nozzle securing nut is loose

Securely, tighten up the nozzle cylinder.

O-ring and O-ring retainer areassembled in reverse order

Replace the O-ring and O-ring retainer
by following the instruction on thismanual.

Grease in the hermetic part/
airtight part is depleted

Apply grease by following the
instruction on this manual.

The nozzle securing nut is loose

Securely, tighten up the nozzle securingnut.

The nozzle is loose

Replace the nozzle cylinder set

The O-ring is loose

Replace the O-ring.
※Replace the nozzle cylinder set for 2µL
and 10µL.

The piston lip is loose

Replace the piston lip.

The tip is loosely attached.

Reattach the same loosened tip, or witha new one firmly.

Grease in the hermetic part/
airtight part is depleted

Apply grease by following the
instruction on this manual.

The liquid has aspirated andleaked inside the nozzle cylinder.

If the push button does not work wellafter dispensing, or it is sticking to thebody, disassemble the pipette andwash/clean every part (or wipe down
every part with a soft cloth). If someparts get rusty or corroded inside thebody, replace the part(s) with newone(s).

Grease in the hermetic part/
airtight part is depleted

Apply grease by following the
instruction on this manual.

Push button moves stiffly.

When the pipette cannot be fixed after examining and conducting the above
mentioned procedure, immediately stop using the pipette and ask us or our
agent to repair it.
Before bring the pipette for repair, be sure to check whether it has been
contaminated with microbes, and/or harmful or toxic substance. Otherwise, itcannot be undertaken the repair.

- 38 -

* 8. Replacement parts list

8.1 Consumables● Premium Tip (Bulk tip, Autoclavable)
CodeApplicable models

Length of tip(External size)

Q’ty

00-BMT2-UT

2µL、10µL

31.5mm

1000

00-BMT2-SG

20µL、100µL、200µL

53.0mm

1000

00-BMT2-K

300µL

58.9mm

1000

00-BMT2-LG

1000µL81.9mm

1000

00-BMT2-X

5000µL

132.0mm200

00-BMT2-ZS

10000µL

171.2mm200● Premium Tip (Racked tip, Autoclavable)
CodeApplicable models

Q’ty

00-BMT2-UTWR 2µL、10µL

960 (96pcs x 10 cases)

00-BMT2-SGR

20µL、100µL、200µL

960 (96pcs x 10 cases)

00-BMT2-LGR

1000µL

960 (96pcs x 10 cases)

00-BMT2-KR

300µL

960 (96pcs x 10 cases)

00-BMT2-XR

5000µL

100 (50pcs x 2 cases)

00-BMT2-ZSR

10000µL

80 (40pcs x 2 cases)

8.2 Spare parts list(1)Single channel
Code

Replaced parts name

Remarks―――――――

Volume
20uL/100µL/200µL
1000uL

00-NAR-G1000

Seal spring

00-NAR-ABC2Nozzle cylinder set

2µL

O-ring and O-ring retainer
2µL
included

00-NAR-ABC10

Nozzle cylinder set

10µL

O-ring and O-ring retainer
10µL
included

00-NAR-A20

Nozzle cylinder set

20µL―――――――

20µL

00-NAR-A100

Nozzle cylinder set

100µL―――――――

100µL

00-NAR-A200

Nozzle cylinder set

200µL―――――――

200µL- 39 -

00-NAR-A1000

Nozzle cylinder set

Code1000µL―――――――

Replaced parts name1000µL

Remarks

Volume00-NAR-A5000

Nozzle cylinder set

5000µL―――――――

5000µL

00-NAR-A10000

Nozzle cylinder set

10000µL―――――――

10000µL

00-NAR-E10

Ejector pipe

2µL/10µL―――――――

2µL/10µL

00-NAR-E200

Ejector pipe

20µL/100µL/200µL―――――――

20µL/100µL/200µL

00-NAR-E1000

Ejector pipe

1000µL―――――――

1000µL

00-NAR-E5000

Ejector pipe

5000µL―――――――

5000µL

00-NAR-E10000

Ejector pipe

10000µL―――――――

10000µL

00-NAR-C20

O-ring retainer

20µL―――――――

20µL

00-NAR-C100

O-ring retainer

100µL―――――――

100µL

00-NAR-C200

O-ring retainer

200µL―――――――

200µL

00-NAR-C1000

O-ring retainer

1000µL―――――――

1000µL

00-NAR-B20

O-ring

20µL―――――――

20µL

00-NAR-B100

O-ring100µL―――――――

100µL

00-NAR-B200

O-ring200µL―――――――

200µL

00-NAR-B1000

O-ring1000µL―――――――

1000µL

00-NAR-B5000

Piston lip

5000µL―――――――

5000µL

00-NAR-B10000

Piston lip

10000µL―――――――

10000µL

00-NAR-F15000

Nozzle filter (10pcs)―――――――

5000µL /10000μL- 40 -

(2)Multi channel
Code

Replaced parts name

Remarks

Volume00-NAR-E10M8Ejector unit 10µL 8ch―――――――

10uL

00-NAR-E300M8Ejector unit 300µL 8ch―――――――

100uL/200uL/300uL

00-NAR-E10M12Ejector unit 10µL 12ch―――――――

10uL

00-NAR-E300M12 Ejector unit 300µL 12ch―――――――

100uL/200uL/300uL

Remarks

Volume(3)Common parts
Code

Replaced parts name00-NAR-GRS

Grease―――――――

All

00-NAR-AT

Volume adjustment tool―――――――

All- 41 -

Memo- 42 -

Please note that the specifications of the accessories may be changed without notice.
Always check our website for the latest specifications and information.
※Copying/Reprinting the manual in whole or in part without permission, is prohibited by law.

Inspection and Calibration Statement
The enclosed pipette was tested and calibrated under closely controlled environmental conditions
to ensure that it meets published calibration specifications. The precision and accuracy results
obtained for this pipette are provided on the enclosed calibration certificate. Because temperatureand humidity conditions affect the calibration results of liquid measurement devices, yourpipette should be calibrated under conditions of use. The calibration results obtained in yourlaboratory may vary from our results, due to differences in the environmental testing conditions.

Information on repair or servicing, when contacting your local distributor.

Authorized Representative：
Emergo Europe B.V.
Prinsessegracht 202514 AP, The Hague The Netherlands

Manufacturer：

Head office & Factory
2760-1 Nishikata, koshigaya-Shi, Saitama 343-0822, Japan
TEL: +81-48-989-1301 FAX: +81-3-6273-7944
Tokyo office (Sales Division)
PMO Kandaiwamoto-cho Ⅱ 4F, 2-2-4 Iwamoto-cho Chiyoda-ku,
Tokyo 101-0032 Japan
TEL: +81-3-5829-4539 FAX: +81-3-5829-4504
URL: http://www.nichiryo.co.jp/
E-mail: i-sales@nichiryo.co.jp- 43 -