Nichipet ECO
Pipette with Glass Tip for Endocrine Disruptors Research

User's Manual

CERTIFIED
ISO9001• Thank you for your purchase of our Nichipet ECO.
• Before proceeding to work with Nichipet ECO, carefully read this manualfor proper use of it.

Always Pursuing Originality
Since 1944

Nichipet ECO
Digital micro pipette for liquid handling
Features● Glass Tip enables totally bias free liquid handling for endocrine disruptors analysis.
● Nozzle with double O-ring enables easy tip fitting/ejection and reliable sealing
secured.
● The sample volume can easily be set by simply turning the push button whilelooking at the digital indication.
● The instrument has a special structure so that its precision is hardly affected by
the temperature of your hands if the instrument is used for long hours.
● Since PTFE (fluoroplastic) is used in the airtight chamber of the instrument, theinstrument keeps air tightness and precise reproducibility for long hours.
● Ceramic (plunger), PTFE (Tip holder), and FPM (O-ring) are used for liquidcontacting parts.
● Setting of the sample volume can be locked with locking mechanism so that thevolume does not easily change.
Standard accessories● Glass tips ................ × 1● O-rings for nozzle .... × 2❋ When unpacking the package, check to make sure that the above-mentioned
items are included.
Precautions on safety● For using your Nichipet ECO properly and safely, carefully read "Precautions on
safety" in this paragraph and "CAUTIONS" on the next page before starting workwith it.
● The contents of "CAUTIONS" are matters that require the user's attention, notonly for using the Nichipet ECO properly but for preventing the user from accidents
and physical damage.
● After reading this manual, please keep it in a convenient place for refering to at
any time.

–1–Please read the following prior to use for your safety and correct usage.

CAUTIONS
Be sure to observe the following instructions for using yourNichipet ECO properly and safely.
If the user uses the Nichipet ECO in the wrong way, disregarding the following
instructions, it may result in injury to the user or/and other persons or physical damage
to this instrument or/and other equipment.
1. Don’t use this instrument for any purpose except pipetting / fractionzing liquid.
2. Don’t modify this instrument, because modification may cause an accident.
3. Don’t use this instrument for pipetting any liquid to be injected into the humanbody.
4. Don’t discharge any sample liquid while pointing the instrument at anybody,
because some kinds of liquid are harmful to humans.
5. Firmly fit the tip to the nozzle, otherwise the tip may fall off and the inside sample
liquid may scatter.
6. Carefully handle the glass tips. They are very dangerous, if broken.
7. If the instrument is soiled with liquid harmful to the human body, immediately
take proper measures to clean it safely before proceeding with work.
8. When handling liquid harmful to the human body , be careful not to directly touch
the tip during or after use.
9. Don’t use this instrument for stirring liquid and so on, otherwise not only the tip
may be loose and fall off but the instrument may be soiled with the scatteredliquid.
10. Although this instrument has good chemical resistance in general, it may bedamaged by some kinds of chemicals such as N-methyl-pyrrolidinone etc.
When using a special chemical, please make enquires to our company.

–2–Matters that require strict observance
Users are required to strictly observe
the following points in order for
the instrument to keep its excellent precision,reproducibility and original performance for a long time.
1. Don’t expose this instrument directly to the sun when working with it or for 2
hours before starting work, otherwise the instrument may fail in precise pipetting.
Avoid working with this instrument in a humid and hot place.
2. Just before starting work with this instrument, avoid touching the tip and nozzle
cylinder as far as circumstances permit. If the nozzle cylinder is warmed by yourhand, it is hard to maintain accuracy.
3. For fractional pipetting, follow the forward method (the way explained in thismanual). If it is performed in a different way, it may result in inaccurate pipetting.
4. Operate the push button very gently. If it is quickly released, it may result in not
only inaccurate pipetting but also deterioration in the instrument, because thesample liquid is sucked into the main body.
5. Don’t reuse a glass tip that has been used once without washing. If a glass tip isrepeatedly used, it may cause inaccurate and impure pipetting and crosscontamination (✽) among samples.
(✽) For example, if the previous sample liquid remains inside the tip, it is mixed
in with new sample liquid and the new sample is contaminated by the previous
one. Therefore, pipetting of the next sample ends with a wrong result. Thisphenomenon is called mutual contamination of samples.
6. Don’t hold the instrument horizontally or upside down when there is liquid inside
the tip, otherwise the liquid gets into the main body and the instrument maydeteriorate.
7. When turning the push-button, don’t exceed the specified sample volume limit,
otherwise the instrument may be damaged or deteriorate.
8. Don’t perform pipetting with less liquid than the set volume. If the quantity ofliquid is less than the set volume, it may cause the liquid to scatter into the main
body and the instrument may deteriorate in quality.

–3–Operating procedure
1. Setting the liquid volume
1) Turn the lock screw in the unlocking direction to loosen it. (Fig. A)
2) Turn the push button to set the digital counter to a desired liquid volume. To
increase the setting volume, turn the push button to pass the last set graduationon the scale by half a turn and then set at the higher graduation as desired. To
decrease the setting volume, reset the digital counter directly at the lower graduation as desired. When setting the liquid volume, set the counter's graduation at
the point mark appearing in the lower part of the counter window. (Fig. B)
3) After setting the liquid volume, turn the lock screw in the locking direction to lockit. (Fig. A)

Note: Don't exceed the specified liquid volume limit, otherwise theinstrument may be damaged or deteriorate in the quality.

2. Extracting liquid
1) Attach a glass tip to the nozzle.
2) Press the push button down from point "a" to the point "b". (Fig. C)
3) While depressing the push button, immerse the tip into the liquid to the extracting
volume (approximately 3 mm deep). (Fig. D-1)
4) Pull up the push button to point "a" to suck the liquid into the tip. With the tip still
immersed in the liquid, keep the instrument stationary for about 1 second to wait
untill the liquid is completely sucked into the tip. (Fig. D-2)
5) Gently extract the instrument to separate the tip from the liquid so that there are
no drops of liquid left on the outside of the tip. If there are some drops left on theoutside of the tip, wipe them off with a tissue, or the like, taking care not to touch
the tip.

Note: Don't extract liquid with the push button depressed at point "c"shown in the Fig. C.
Note: Be careful to operate the push button very gently. If it is rapidly
released, the liquid may possibly be sucked into the main bodyand pipetting may end with an inaccurate result.

3. Discharging the liquid
1) Gently place the tip on the inner wall of a proper vessel. (Fig. D-3)
2) Gently press the push button down from point "a" to point "b". Five seconds later,press down the push button again from point "b" to point "c". The liquid is discharged from the tip. (Fig. D-5)

When handling liquid harmful to humans, be careful not to touch
the tip during and after operation.

–4–To lockPush button

To unlock
Lock screw

Fig. A

Nichipet ECO 1000µR
Digital counter 100~1000µR

01

1

00
0

00
Point markFig. B–5–

abc

Fig. C12

3

4Fig. D
–6–

56

Disassembling / Reassembling the airtight chamber
If such symptoms as mentioned in “Troubleshooting” (page12) occurs, disassemble
and inspect the instrument according to the following procedures.

1.Disassembling
1 Turn the nozzle cylinder counterclockwise to remove it, as it is screwed into themain body.
Note: When removing the nozzle cylinder, take care with the internal parts assome of them occasionally spring out of the body (for types of 0.2mR
R to1mR
R capacity).
2 Remove internal parts one after another• 0.2mR,1mR:Fig. E
Remove the plunger, single spring, O-ring retainer, O-ring from the nozzle cylinder.
• 5mR, 10mR: Fig. F, Fig. G
Remove the O-ring retainer and seal ring from the nozzle cylinder.
Note: Take care not to lose small parts during disassembling.

2. Reassembling
1 Reassemble the nozzle cylinder.
• 0.2mR, 1mR: Fig. EFirst set the single spring on the plunger, next set the O-ring retainer, O-ring inthis order. Then, insert the assembled plunger into the nozzle cylinder and screw
it into the body.
• 5mR, 10mR: Fig. F, Fig. G
Set the seal ring, O-ring on the plunger in this order, and then insert the assembled
plunger into the nozzle cylinder while taking care that the O-ring does not come
off the center. After insertion, screw the nozzle cylinder into the body.
Note: When reassembling, be careful not to put the seal ring and O-ring in thewrong order. If they put together in the wrong order, it not only affects
air tightness but causes liquid to leak, inaccuracy, failure in extracting
liquid, etc.
Note: After reassembling, repeat trial operation several times and check to
see that there is nothing wrong with the instrument.

–7–

3. Replacement for O-ring on nozzle
1 Insert the O-ring replacing tool (such as a ^ shaped driver) between the O-ringand the nozzle. Remove the o-ring from the nozzle. (Fig. H)
2 Attach the new O-rings to the nozzle.
Note: Be careful not to damage the nozzle when replacing O-rings with thetool.

^ DriverNozzle O-ring
Fig. H–8–

200µR, 1000µR

Body

Nozzle cylinder

Plunger head

Nozzle O-ringSingle spring
PlungerO-ring retainer

Fig. E–9–

5000µR

Body

Plunger head

Plunger assembly

Plunger
Seal ringO-ringNozzle cylinder

Nozzle O-ring

Fig. F– 10 –

10mR
R

Plunger head

Body

Plunger assembly

Plunger
Seal ringO-ringNozzle cylinder

Nozzle O-ring

Fig. G– 11 –Troubleshooting

The instrument fails to
extract liquid.

The nozzle cylinder hascome loose.

Try to screw the nozzle cylinderfirmly into the body again.

The O-ring / seal ring set
are assembled in thewrong order.

Reset the O-ring / seal ringaccording to the instructions in
disassembling / reassembling

The O-ring / seal ring set
are worn.

Replacement parts:
Purchase nozzle cylinder set for
0.2ml and 1ml for replacement.
Purchase O-ring/seal ring set for
5ml and 10ml for replacement.

The nozzle cylinder hascome loose.

Screw the nozzle cylinder firmly
into the body again.

The nozzle cylinder isworn (stepped wear canbe checked by eye.)Replacement parts:
Purchase nozzle cylinder set for
0.2ml and 1ml for replacement.
Purchase nozzle cylinder for
5ml and 10ml for replacement.

The O-ring / seal ring areworm, because theplunger is damaged orrusty.

Replacement parts:
Purchase plunger set or/andnozzle cylinder set for 0.2ml and1ml for replacement.
Purchase plunger assembly or/
and O-ring /seal ring set for 5ml
and 10ml for replacement.

The tip is loosely attached.

Try to attach the tip to nozzle
tightly again.

The nozzle O-ring is wornor damaged.

Replace the nozzle O-ring withnew one.

Liquid has been sucked
into the main body.

If the push button does not work well justafter extracting liquid or it is just sticking tothe body, disassemble the instrument andwash/clean every part (or wipe down every
part with a soft cloth). If there are someparts getting rusty or corroded inside thebody, replace the parts with new ones bypurchasing optional replacement parts.

Extracted liquid leaks
from the tip.

The push button operates
poorly.

If there is still something wrong with the instrument after checking the above-mentioned
item, immediately stop using the instrument and ask us or our agent to repair it.
Before bringing the instrument for repair, be sure to check whether it has been polluted
with microbes or matter harmful to humans. In case it has been polluted, be sure to giveit a sterile treatment such as autoclaving.

– 12 –

Code

Variable capacity Measured volumeR)R)(mR(mR00-NPC-200

0.02~0.200-NPC-1000

0.1~1.000-NPC-5000

1.0~5.000-NPC-10ML

1.0~10.0

Accuracy(%)

Precision(%)±6.0±3.0±5.0±3.0±5.0±3.0±6.0±3.0<6.0<3.0<5.0<3.0<5.0<3.0<6.0
<3.0

0.02
0.2
0.1
1.0
1.0
5.0
1.0
10.0

Note: The value of accuracy and precision is made with distilled water at room
temperature 22°C~23°C● Tip
Code00-GLT-S00-GLT-L00-GLT-X00-GLT-Z

Usable model
0.2mR 00-NPC-200
1mR 00-NPC-1000
5mR 00-NPC-5000
10mR 00-NPC-10ML

Q’ty (box)10101010● Replacement parts list (when placing order, make sure tospecify the capacity of each item.)
Name of replacement part
1. Plunger set

Contents of set capacity
Plunger head / plunger

2.

Plunger Assembly

Plunger / Joint block
Joint plate
Joint shaft

3.
4.
5.

Single spring
Nozzle cylinder
Nozzle cylinder set

6.
7.

O-ring / Seal ring set
Nozzle O-ringNozzle cylinder / O-ring
Seal ringO-ring / Seal ringNozzle O-ring (2pcs)

8.

Nozzle O-ringNozzle O-ring (2pcs)

Capacity (mR)
0.2mR
1mR
5mR
10mR
Common5mR, 10mR
0.2mR
1mR
5mR,10mR
0.2mR
1mR
5mR,10mR❋ Specifications of the instruments and optional accessories as well as contents ofaccessory sets are subject to change without notice.
❋ No part of this manual may be reproduced/reprinted in any form without permissionof the copyright holder. The copyright law strictly prohibits this.

– 13 –

Inspection and Calibration Statement
The enclosed pipette was tested and calibrated under closely controlledenvironmental conditions to ensure that it meets published calibration specifications.
The precision and accuracy results obtained for this pipette are provided on theenclosed calibration certificate.
Because temperature and humidity conditions affect the calibration results of liquidmeasurement devices, your pipette should be calibrated under conditions of use.
The calibration results obtained in your laboratory may vary from our results due todifferences in environmental testing conditions.

– 14 –

For repair, service or information you may contact your local distributor.

MANUFACTURER:

1-10-1 Kandanishiki-cho, Chiyoda-ku, Tokyo 101-0054, Japan
TEL: 81-3-6273-7652(English) FAX: +81-3-6273-7944
Homepage URL http://www.nichiryo.co.jp
E-mailinfo@nichiryo.co.jp
2012.Ver.1