Digital micro pipette for liquid handling

User’s Manual

*SB-NLE002ER 2022/6/10
SB-NLE001ER 2022/4/15

Thank you very much for purchasing NICHIRYO Le.
Please read this manual carefully before using this device.
Safety Precautions








Please read this manual carefully and have sufficient understanding of thecontents and instructions, especially concerning matters of safety, prior touse.
The notes stated here is for the safety of the user, and for the correct
usage of the product.
Contents marked with
“Danger Level Symbols” are matters that
require the user’s utmost attention, not only for using Nichiryo Le properly,
but also to prevent users from injuries or death, harm to others, and/or
property damage.
After reading this manual, please keep it in a noticeable and accessible
place for ‘users of the device’ to refer to at any time.
Any serious incidents that has occurred in relation to the equipment shallbe reported to the manufacturer and to the competent authority of theMember State in which the user and/or the patient is established.

Danger Levels
DANGER

Will lead to serious injuries or death.

WARNINGMay lead to severe injuries or death.

CAUTION

May lead to light to moderate injuries, and/or cause
property damage.
User information

Caution on disposal of this product
When disposing the pipette body and tips (including adhering liquid), please
comply with the laws and regulations of each country related to disposal, or local
ordinance or regulation.

－ 1－

Digital micro pipette for liquid handling

Table of Contents
1. Product overview
1.1 Features
1.2 Standard Accessories (Included)
1.3 Main illustration

3
3
3
3

2. Information on safety
2.1 Intended Use
2.2 Warnings for Intended Use
2.3 Material information

4
4
4
5

3. Operation/Operating procedure
3.1 Volume setting
3.2 Aspiration of liquid (Forward technique)
3.3 Dispensing the liquid
3.4 Recommendation for accurate pipetting (Technique)

6
6
7
8
9

4. Maintenance
4.1 Cleaning
4.2 Disassembling
4.3 Reassembling
4.4 Sterilization

10
10
10
11
13

5. Specifications (Accuracy/Precision)

13

6. Volume measurement

14

7. Troubleshooting

16

8. Replacement Parts List
8.1 Consumables
8.2 Spare parts list

17
17
17

－ 2－

1. Product overview
1.1 Features







The newly designed curvature and roundness in shape is ergonomic, and mitigates theuser’s fatigue and stress levels from long periods of use.
Easy to read digital indicator.
The sample volume can easily be set by simply rotating the push button.
A wide range of sampling volume can be covered by five models, from 1μL to 1,000μL.
Patented body construction shields the hand temperature permeating through the bodyand inner workings of the device, which increases the accuracy of the volume measurements.
The tip can be removed without direct contact, by simply pressing the tip ejector.

1.2 Standard accessories (Included)
Accessories

QTY
3

Tip
Grease1

User’s Manual

1

When unboxing the package, check that all of the items above are included forthe respective model.

1.3 Main illustration

Push button
Ejector button
Volumeindicator

Main body

Ejector pipe

－ 3－

2. Information on Safety
2.1

Intended Use

Nichiryo Le, used in conjunction with pipette tips recommended by Nichiryo, aredesigned and constructed for low-contamination transfer of liquids, especially for samples
from the human body and for reagents within the scope of an in-vitro diagnostic
application in order to allow the in-vitro diagnostic medical device to be used as intended.
Therefore, Nichiryo Le is subjected to the accessories of in-vitro diagnostic medical
devices under Regulation (EU) 2017/746. The accessories is treated as in-vitro diagnostic
medical devices in their own right under Regulation (EU) 2017/746.
Nichiryo Le are intended for operation by qualified staff.

2.2 Warnings for intended use
DANGER








When handling radioactive substances or infectious substances, always check andconfirm the information first, and follow guidelines on their safety procedures.
When using harmful liquids to the human body, be very careful in the handling of thesubstances.
Never touch or come into direct contact with the used chips.
Never touch filters directly that are contaminated by harmful or toxic substances.
When liquids that are harmful to the human body adheres and/or contamination occurs,
use appropriate measures to clean and decontaminate the device before continuing
its use.
This product is not intended for use on living organisms.

WARNING
Do not eject the tip with liquid inside of it.
Do not eject the tip towards anybody.
Do not expel or dispense any liquids towards anybody.
Depending on the splashed liquid, there is danger of causing injuries to the humanbody.
Please protect yourself in accordance with the general procedure of danger prevention,such as wearing protective clothing, protective glasses and gloves.





－ 4－

CAUTION
Do not use the pipette for any other purpose, use only for pipetting and liquid dispensing.
Do not modify the pipette, modification can lead to accidents.
Do not stab the tip into the human body, or eject the tip towards anyone. The tip isvery sharp and extremely dangerous.
The filter replacement tool tip is very sharp and can be dangerous. Please handle itwith caution.
Since the main body of the pipette becomes extremely hot right after autoclaving anddrying. In this state, please do not touch it directly with your hands. It can lead to
accidents and burn injury.
Do not use the pipette for any purpose other than pipetting and liquid dispensing,such as stirring liquid with this product. It can lead to loosening of the tip, the tip
dropping off, liquid adhesion and contamination to the main unit of the device andaccidents and/or injury.
During operation and maintenance, if any worn, missing or broken parts are detected,
discontinue use immediately. Order and replace the part or parts, before use.










2.3

Material information

CAUTION

The use of highly reactive chemicals may damage the device. Pleaseacknowledge the following materials that are used, and do not use liquids that will
violate them.

Material of external parts
 Polypropylene (PP) Polyetherimide (PEI) Polyphenylenesulfide (PPS) Polycarbonate(PC) Nitrile rubber (NBR)
 Stainless steel－ 5－

* 3. Operation/Operating procedure
Users of the device are required to strictly observe the following in orderfor the pipette to keep its excellent accuracy, precision and original
performance.
Do not expose pipette to direct sunlight when using it, or for 2 hours prior to use,otherwise the pipette may lose its accuracy. Avoid working with pipettes in a hightemperature, low temperature, low humidity environment, or when the temperature
difference between the environment including the main body of the device and theliquid is large, accuracy and precision may not be guaranteed.
This Pipette can be used in a stable environment between +4°C and +40°C, but
the specifications may vary.
Just prior to use, avoid touching the tip or nozzle cylinder as much as possible.
If they are warmed up, accuracy may not be obtained.
Original Nichiryo tips are recommended. Nichiryo Le is calibrated with the original
Nichiryo tip, if you use other tips (Premium Tip), deviations in original factory settings may occur, and accuracy will not be guaranteed.
Depending on the frequency of use, the pipette should be cleaned and the airtightchamber should be maintained according to the manual.










3.1 Volume setting
1)

2)
3)

Turn the push button to set the volume indicator to a desired liquid volume.
When setting the liquid volume, set the indicator’s minimum graduation scale to the triangle
point marker in the lower part of the volume indicator (Fig. A). Please refer to the volume
indicator and minimum graduation unit for each model to set the measurement volume
accordingly. The volume indicator numbers are colored in black, or in red, to indicate theposition of the decimal point. For 10μL and 20μL models; scale units are in (μL),
the integer is indicated in black, and the decimal is indicated in red. For 100μL and200μL models; the scale units are in (μL), there is only integer, and is indicated inblack. For 1000 μL(1mL) model, scale unit is in (mL), the integer is indicated in red,the decimal is indicated in black.
To increase the volume setting, turn the push button till it passes the designated volume
setting by at least 1/3 of a rotation of the push button dial, and then dial back to set
the designated volume.
To decrease the volume setting, simply turn it to the designated volume directly.

Volume indicator

Fig-A
10μL
NLE-1020μL
NLE-20100μL
NLE-100

200μL
NLE-200

1
0

2
0

1
0
0

2
0
0

10uL
(0.02uL)20uL(0.02uL)

100uL(0.2uL)200uL(0.2uL)

Pipette1000μL typeNLE-1000001000uL
1mL
Minimum(2uL) Minimum
scale(0.02mL)
graduation
unit

CAUTION

Do not exceed the specified liquid volume limit, otherwise the pipette may bedamaged or deteriorate in its quality.
Never rotate the push button dial more than 1/3 of a turn at maximum volume
setting. Damage to the device can occur, but also accuracy/precision may be impired.
－ 6－

3.2 Aspiration of liquid (Forward technique)1)Attach a new tip to the nozzle end.



It is recommended that tips are directly picked up from the rack. Do not twist
the pipette when fixing tip on.

Please be sure to mount the tip on the main nozzle securely.
may cause the tip to drop off and liquid to splash.

Failure to do so

CAUTION
Do not perform pipetting with less liquid than the set volume. If the quantity of liquid isless than the set volume, it may cause the liquid to spray into the main body, and thepipette may be damaged or deteriorate in its quality.
2)
3)

Press down the push button fully to the first stop position “b” (Fig. B) – ①.
Hold the pipette vertically and immerse the tip 2mm. to 3mm. below the surface ofthe liquid (Fig. C) - ①.
4) To aspirate the set volume of liquid into the tip, release the push button slowly andlet the push button go back to the initial position naturally. It takes 1 second to aspirate
the liquid. During this operation, stop to wait for the suction process of the liquid to
be totally completed, making sure that the liquid is drawn up into the tip withcertainty (Fig. C) -②.
5) Draw the tip of the pipette carefully vertically upward and away from the liquidsurface, then touch the tip to the side of the tube to remove excess droplet adheringto the outside of the tip. (Fig. C) - ③


Do not aspirate when the push button is at ‘position ④’ (Fig. B).
We recommend using the forward technique as the operation method of thisdevice for pipetting. Nichiryo Le has been calibrated with the forward method,and precision may not be obtained when dispensing by any other means.
Always change the tip when using different liquids to avoid cross-contamination.

CAUTION

Please operate the push button slowly and gently.
Sudden release of the push button, can lead to the aspiration of the liquid into the nozzle
cylinder; not only precise accuracy will not be obtained, but also the quality of the device
will be impaired. Due to the larger base volume measurements of models, 1000μL,
there is a higher chance of the above to occur. To eliminate this possible situation that
leads to inaccuracy, precision failure or contamination.
When using the pipette, be sure to always attach and use a tip. Failure to do so, willlead to liquid entering the inside of the device’s nozzle cylinder and cause serious
malfunctions
Never turn the device sideways or upside down with liquid in the tip. Liquid can enter
inside the device’s nozzle cylinder, which can cause ※contamination and breakdown.

※Cross-contamination with the entered liquid remaining inside the main body of the device

can also occur, when switching liquids thereafter.
－ 7－

3.3 Dispensing the liquid
1) Touch the end of the tip against the inside wall of the recipient tube at a slight angle.
2) Press the push button down slowly and smoothly to the first stop “position b” (Fig. B)-③.
Wait for a second, then press the push button down to the second stop “position c” (Fig. B)-④
to expel the last droplet of liquid from the tip (Fig. C) –④ and ⑤.
3) Keep the push button compressed. Remove the last droplet by slightly touching andrunning the end of the tip up against the inner wall of the tube, then move the tip of thepipette vertically straight up and out of the tube.
4) Press the ejector button to detach the tip to dispose (Fig. C)-⑥.


We recommend the tip to be disposed of after each use.
Repeated use may lead to and result in; loss of accuracy, precision, and maylead to contamination/cross-contamination due to adhesion and deposits fromprior use.

①

②

③

④

a
b
c

Ejector button

Fig. B

①

②

③

a
b
C

Fig. C
－ 8－

④

⑤

⑥

3.4 Recommendation for accurate pipetting (Technique)
In addition to the previously mentioned operations of pipetting, the following
technique maximizes the performance of the pipette.
1) Pre-Rinsing the tip
Higher precision can be obtained by performing the pre-rinsing of the tip. When using
a fresh pipette tip for the first time and before sampling, it is important to pre-rinse the tip
at least two to three times with the target solution before pipetting. Repeat the aspirationand discharge to the position of the 1st stop consecutively for two to three times.
When strict precision is required, this method is recommended for all kinds of liquid handling.
Pre-rinsing is a fast and easy way to increase the accuracy.
2) Handling of High Density Solutions / Viscous Solutions
After aspirating the liquid into the tip, wait for 2 to 3 seconds before removing the tip
slowly from the surface of the liquid. When dispensing, wait 2 to 3 seconds at the firststop position before pushing into the second stop position.
3) Small volume dispensing
Especially for volumes less than 50 μL, please operate the pipette slowly and smoothly.
Also, please pay close attention to the effect of evaporation loss, due to temperature andhumidity.

－ 9－

4. Maintenance
4.1 Cleaning
When Nichiryo Le’s exterior is soiled, please use 70% ethanol on a clean soft cloth to wipe it
off. Also, if any symptom that is described in the "7. Troubleshooting" section occurs,
disassemble and inspect the device (each part), and then check and follow theprocedures in the ‘Troubleshooting Table’ to identify and isolate, correct and/or fix the situationor problem.

CAUTION

Use of highly reactive liquids, may damage the device.
After using a highly reactive liquid or/and if contamination occurs, perform the disassemblyand cleaning of the device, accordingly to the following method in this manual.

In order to keep and use Nichiryo Le in its best condition, we recommend theuser carries out; periodic checks and maintenance, and volume
inspection/calibration of the device.

4.2 Disassembling
1) Remove the ejector pipe.
While pressing the ejector button, shift the ejector pipe to the right (clockwise turn)and remove it from the ejector shaft (Refer to Fig. D).
2) Remove the bottom nut.
While holding the nozzle cylinder firmly, turn the bottom nut counterclockwise anddetach it from the body.
3) Remove each part.
Remove and disassemble each part in the nozzle cylinder in the following order, firstspring, plunger, first spring holder, second spring holder, O-ring. Once disassembled,
carry out inspection and cleaning of the airtight section. When needed, please
replace parts.
Please be careful not to lose any small parts during disassembly.

CAUTION

When removing the nozzle cylinder, the internal parts may spring out and apart fromthe reaction of the loaded spring inside, be mindful of this point, and take precautions
when unscrewing and taking apart the nozzle cylinder.



Depending on the Model, the specification of the O-ring retainer will be different.

－ 10－

4.3 Reassembling✱ Assembly is done in the exact reverse order of disassembly. (Refer to Fig. E).
1) Set the O-ring onto the O-ring retainer.
2) With the plunger tip facing upward, attach and assemble each part in the following order,
first spring, first spring holder, stroke pipe, second spring, O-ring retainer, O-ring.
Then, insert the assembly into the nozzle cylinder.
3) The whole assembled nozzle cylinder is then attached onto the main body. Turn thebottom nut clockwise slowly and carefully.
4) Attach the ejector pipe. Press the ejector button to expose shaft, attach and rotate theejector pipe counterclockwise.
After reassembling, perform several tests to inspect the pipette’s function/reliability.
When screwing the nozzle cylinder into/onto the main body, hold the parts
vertically as a visual reference, and slowly turn to let the threads bite together
correctly. If screwed in an offset way or with force, the O-ring will be unseated,
stripping of the threads and/or damage to the casing can occur, all of which, willcause leakage.

－ 11－

Press ejector button

Clockwise turn:
LoosenCounterclockwise turn:Tighten

7. Stroke pipe

Circled Numbers in (Fig. E) corresponds to Parts Numbers in the replacement parts list.

－ 12－4.4 Sterilization

The nozzle cylinder and the ejector pipe can be autoclaved at 121°C for 20 minutes.
Also, ethanol on a soft clean cloth can be used to wipe the outer body.

CAUTION
Only autoclave the nozzle cylinder and the ejector pipe.
Subjecting other parts to the autoclave will lead to deformation and damage.

Do not use UV sterilization.
UV rays will cause deterioration and malfunction to the device* 5. Specifications (Accuracy/Precision)

Maximum Permissive Errors.

Table-1
Pipette types(Code)

10μL(00-NLE-10)

Volume
range
(μL)

1～10

20μL
(00-NLE-20)

2～20

100μL
(00-NLE-100)

10～100200μL(00-NLE-200)

1000μL(00-NLE-1000)

20～200100～1000

MeasuredVolume(μL)

Accuracy(systematic error)(%)

Precision(random error)
(%)

1

±6.0★

≦5.0★

5

±2.0

≦1.6

10

±1.2

≦0.8

2

±5.0★

≦3.0★

10

±2.0

≦1.0

20

±1.0

≦0.5

10

±2.0

≦1.0

50

±1.2

≦0.6

100

±0.8

≦0.3

20

±1.2

≦1.0

100

±1.0

≦0.6

200

±0.8

≦0.3

100

±1.2

≦0.8

500

±1.0

≦0.6

1000

±0.7

≦0.3

Accuracy and precision of 1μL of “10μL model” and 2μL of “20μL model” are greatly
affected by the user’s sampling skills, and/or by various factors and conditions.
★

・
・
・
・
・

Tips used: Premium Tip (BMT2)
Measurement temperature: Between 20°C to 25°C
Relative humidity: above 50%
Measurement medium: distilled water
Barometric pressure, 101kPa
Volume measurement is in accordance with ISO 8655-6.

－ 13－

6. Volume Measurement* 1)

In order to avoid influence from temperature differences, ready the pipettes forinspection, the distilled water, the balance and tips 2 to 3 hours before use, in theenviron where the measurement is to be conducted to attain temperature equilibrium.
※
The measurement room should be in a controlled temperature between 20°C to
25°C, and the measurement should be held where there is no direct influence from windblowing from an air-conditioner, heater or fan.
Please use the Nichiryo Original Premium Tip Series (volume compatible ones), and be
sure to install it on the pipette from the rack.

2)

Using distilled water as the sample, aspirate and dispense the volume to be calculated
inside the weighing vessel on the balance. Please use a vessel with a lid for theweighing vessel inside the balance. Also, include a small amount of distilled water in thevessel, prior to adjusting the balance and measurement.

3)

Read the mass (mg) measured with the balance, and then convert it with the"Z correction Factor for Distilled Water[table2]" to obtain the dispensed measured volume (μL).
Vi

Vi  mi  Z
4): Volumem i : Measured massZ

: Z correction factor

Add together the 10 volumes delivered and divide the sum by 10 to provide the mean
volume.

n1V   Vi
10 i 1

V : Mean volume

Calculate the systematic error es [%] with the equation below.

es  100 
5)V  V e s : systematic error [%]V s : selected volumes

Vs

Calculate the random error CV [%] with the equation below. Where n is the number ofmeasurements, in this case n=10 .

 V  V 
n

CV 

100

V

i 1

2

i

n 1

－ 14－

CV : random error [%]Table-2

Z correction factor for distilled water

Temperature
(°C)
20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
24.0
24.5
25.0

95
1.00281.00291.00301.00311.0032
1.00331.00341.00361.00371.00381.0039Air Pressure (kPa)
100
101.3
1.00281.00291.00291.00301.00311.00311.0032
1.0032
1.00331.00331.00341.00341.00351.00351.00361.00361.00371.00381.00391.00391.00401.0040－ 15－

105
1.00291.00301.00311.0032
1.00331.00341.00361.00371.00381.00391.00407. Troubleshooting
Symptom

Pipette fails toaspirate liquid.

Extracted liquid leaks
from the tip.

Push button moves
stiffly.

Possible cause

RemedyBuildup of matter/substance on theinside of the cylinder wall.

Clean or replace the nozzle
cylinder.

The nozzle cylinder is loose.

Securely, tighten up the nozzle
cylinder.

The O-ring is worn.

Replace the O-ring.

The O-ring is worn.

Replace the O-ring.

Grease(lubricant) in the airtightsection is depleted.

Grease the plunger and O-ring.

Noticeable wear to the nozzle
cylinder.

Replace the nozzle cylinder.

The plunger is damaged or
rusty.

Replace the plunger.

The tip is loosely attached.

Reattach the same loosened tip,or with a new one firmly.

Grease (lubricant) in the airtightsection is depleted.

Grease the plunger and O-ring.

The liquid has aspirated andleaked inside the nozzle cylinder.

Disassemble the lower parts andclean or replace the part(s) withnew one(s).

Plunger surface is dirty or foreign
matter has adhered to it.

Disassemble the lower part andclean or replace the plunger.

When the pipette cannot be fixed after examining and conducting the above
mentioned procedure, immediately stop using the pipette and ask us or our
agent to repair it.
Before bring the pipette for repair, be sure to check whether it has been
contaminated with microbes, and/or harmful or toxic substance.

－ 16－

8. Replacement parts list
* 8.1 Consumables● Premium Tip (Bulk tip, Autoclavable)
Code

Volume range
(μL)

Color

ApplicablemodelsQ’ty

00-BMT2-SSW

0.5-10

Clear

10μL

1000

00-BMT2-SG

2-200

Clear

20/100/200μL

100000-BMT2-LG

100-1000

Clear

1000μL

1000

ApplicablemodelsQ’ty
960 (96pcs x 10 cases)● Premium Tip (Racked tip, Autoclavable)
Code

Volume range
(μL)

Color

00-BMT2-SSWR

0.5-10

Clear

10μL

00-BMT2-SGR

2-200

Clear

20/100/200μL

960 (96pcs x 10 cases)

00-BMT2-LGR

100-1000

Clear

1000μL

960 (96pcs x 10 cases)

8.2 Spare parts list
Description① Nozzle cylinder

10μL

20μL

100μL

200μL

1000μLNPE-024A

NPE-024B

NPE-024C

NPE-024D

NPE-024E② Bottom nut

NPE-030※

Same for all volume sizes.

③ Ejector pipe

NPE-025A

NPE-025B

NPE-025B

NPE-025B

NPE-025C④ Plunger

NPE-017A

NPE-017B

NPE-017C

NPE-017D

NPE-017E⑤ First spring

NPE-017A

NPE-017B

NPE-017C

NPE-017C

NPE-017D⑥ First spring holder

NPE-020A

NPE-020A

NPE-020A

NPE-020A

NPE-020B⑦ Stroke pipe

NPE-018A

NPE-018A

NPE-018A

NPE-018A

NPE-018B⑧ Second spring

NPE-023A

NPE-023A

NPE-023A

NPE-023A

NPE-023B⑨ O-ring retainer

NPE-021A

NPE-021B

NPE-021C

NPE-021D

NPE-021E⑩ O-ring

NPE-022A

NPE-022B

NPE-022C

NPE-022D

NPE-022E⑪ Grease

N3-0711A※

Same for all volume sizes.

As listed above, replacement parts are specifically designed for each volume size,
please indicate the volume size of your device, when placing your order.

Please note that the specifications of the accessories may be changed without notice.
Always check our website for the latest specifications and information.
※Copying/Reprinting the manual in whole or in part without permission, is prohibited by law.

－ 17－

Memo

－ 18－

Inspection and Calibration Statement
The enclosed pipette was tested and calibrated under closely controlled environmental conditions
to ensure that it meets published calibration specifications. The precision and accuracy results
obtained for this pipette are provided on the enclosed calibration certificate. Because temperatureand humidity conditions affect the calibration results of liquid measurement devices, yourpipette should be calibrated under conditions of use. The calibration results obtained in yourlaboratory may vary from our results, due to differences in the environmental testing conditions.

Information on repair or servicing, when contacting your local distributor.

Authorized Representative：

Emergo Europe B.V.
Prinsessegracht 202514 AP, The Hague The Netherlands

Manufacturer：

Head office & Factory
2760-1 Nishikata, Koshigaya-Shi, Saitama 343-0822, Japan
TEL: +81-48-989-1301 FAX: +81-3-6273-7944
Tokyo office (Sales Division)
PMO Kandaiwamoto-cho Ⅱ 4F, 2-2-4 Iwamoto-cho Chiyoda-ku,
Tokyo 101-0032 Japan
TEL: +81-3-5829-4539 FAX: +81-3-5829-4504
URL: http://www.nichiryo.co.jp/
E-mail: i-sales@nichiryo.co.jp