&
Micro pipette for liquid handling

User’s Manual

CERTIFIED ISO9001●Thank you very much for purchasing Nichipet FⅡ&VⅡ.
●Please read this manual carefully before using.

&Micro pipette for liquid handlingFeatures● New round shape improves friendly handling capability, and mitigates operator’s fatigue fromlong time use.
● Patented body construction avoids permeating hand temperature through the body thatprevents inaccuracy of volume measurement.
● Since PTFE (Fluoroplastic) in sealing compound is used in an airtight chamber, thiscombination keeps airtight and precise reproducibility for long hours.
● Tip can be removed without touching by using the tip ejector.
Standard accessories● Three tips
● Three filters (NPF2-500, 1000, NPV2-L)● An adjustment tool (NPF2-500, 1000, NPV2-L)● User’s Manual● Warranty card
When unpacking package, check to make sure that the above-mentioned items are included.
Precautions on safety● For using your Nichipet Premium LT properly and safely, carefully read “Precautions on safety”
in this paragraph and “CAUTION” on the next page before starting work with it.
● Contents of “CAUTION” are matters that require user’s attention, not only for using Nichipet
Premium LT properly but for preventing user from accidents and physical damage.
● After reading this manual, please keep it in a convenient place for referring to at any time.

-1-Please read following prior to use for your safety and correct usage.

CAUTIONBe sure to observe the following instructions for using
Nichipet FII&VII properly and safely.
If user misuses “Nichipet FII & VII”, or disregards the following instructions, it may result ininjury to the user or/and other persons or physical damage to pipette or/and other equipment.
1. Do not use pipette for any purpose except pipetting/dispensing liquid.
2. Do not modify pipette, because modification may cause an accident.
3. Do not use pipette for pipetting any liquid to be injected into human body.
4. Do not expel dispensed liquid towards anybody.
5. Do not eject tip towards anybody.
6. Do not eject tip with liquid inside.
7. Carefully handle pipette and tip because tip is sharply pointed.
8. Please make sure that the tips are securely attached to the nozzle. Scattering of liquid will
occur if tips fall off the nozzle.
9. If pipette is contaminated with liquid harmful to human, immediately take appropriate
disposal to clean it safely before continuing to work.
10. Do not use pipette for stirring liquid and so on, otherwise not only tip may be loose andfall off but the pipette may be soiled with scattered liquid.
11. Do not touch filters which may be contaminated by harmful substances.
12. It may be damaged by chemicals. Please contact to our company when using strong
chemicals.
13. Since this pipette is not autoclavable, this pipette never can be autoclaved. It may be
deformed or damaged by the heat.
14. Since this pipette is not compatible with UV sterilization, it may be deteriorated ordamaged.
15. For organic solvents, Nichipet EXⅡ Plus (NPLOⅡ) will be recommended to use.
16. Pipette can be used between +4°C and +40°C, but the specifications may vary.
17. Depending on frequency of use, pipette should be cleaned in a soap solution and airtightchamber should be maintained according to this manual.

-2-Note
Users are required to strictly observe the followings in order for thepipette to keep its excellent precision, reproducibility and original
performance for a long time.
1. Do not expose pipette directly to the sun when working with it or for 2 hours before
starting work, otherwise the pipette may lose accuracy. Avoid working with pipettes in ahumid and hot place.
2. Just before starting work with pipette, avoid touching tip and nozzle cylinder as far as
conditions are allowed. If nozzle cylinder is warmed by your hand, accuracy may vary.
3. For pipetting, follow the forward method (the way explained in this manual). If it isperformed in a different way, it may result in inaccurate pipetting.
4. Operate push button very gently. If it is quickly released, it may result not only
inaccuratepipetting but also deteriorated the pipette because sample liquid may bepermeated into the main body. 5. Do not reuse tip that has been used once, and carefully
dispose used tip. If tip is used repeatedly, it may cause inaccurate and impure pipetting
and cross contamination (*) among samples.
* .For example, if previous sample liquid is left inside tip, it is mixed with new sample liquid
andthe new sample is contaminated by the previous one. Therefore, pipetting of the next
sample results wrong. This phenomenon is called mutual contamination of samples.
5. Do not hold pipette horizontally or upside down when there is liquid inside tip, otherwise
the liquid gets into the main body and the pipette may be contaminated.
6. Do not perform pipetting with less liquid than set volume. If the quantity of liquid is lessthan the set volume, it may cause the liquid to scatter into the main body and the pipette
may deteriorate in quality.

-3-Contents

Operating procedure ·························································5
Disassembling/Reassembling the airtight chamber ·····················6Service And Maintenance Information ····································6Disassembly ····································································6Filter replacement procedure ···············································8Specification ···································································9Ordering Information ······················································· 10
Troubleshooting ····························································· 11-4-

Operating procedure
1. Select the desired volume on Nichipet VⅡby turning the thumb knob until the desired
volume figures on the thumb knob correspondto the arrow mark printed on the topof the pipette handle.(Fig. 1)
2. Place a disposable tip firmly onto the endof the pipette. Depress the thumb knob tothe first stop. Dip the tip into the liquid to adepth of 3mm. (Step 1 of Fig. 2)
3. Smoothly return the thumb knob to its originalposition. The liquid will then be drawn
into the tip. (Step 2)
4. Touch the tip against the inside wall of the receiving vessel. (Step 3)
5. Smoothly depress the thumb knob to the first stop (step4), pause: thendepress the knob to the second stop (step5).
Note: When dispensing serum and other viscous fluids, it is necessary to pause
about two seconds before moving to the secondary stop.
6. Remove the used tip from the pipette by depressing the tip ejector knob.
(Step 6)
Note: Depress and release the thumb knob at constant speed to ensure goodprecision.

-5-

Disassembling/Reassembling the airtight chamber
Listed below are some techniques found to improve sampling precision.
PLEASE READ THIS SECTION CAREFULLY.
1. Try to effect the same speed for both the intake and delivery of all samples.
Smooth depression and release of the plunger knob will give the mostconsistentresults. Never allow the plunger to “snap” back. Consistency of technique is akey to precision.
2. Always depress the thumb knob to the proper stop before insertion of the tip
Into the solution. Depression of the thumb knob after insertion may cause theFormation of an air bubble in the tip and result in filling error.
3. Try to insert the tip to approximately the same depth in the sample each time,never going deeper than 3mm. Hold the instrument as vertically as possible(10° maximum from vertical).
4. When sampling hot or cold material, the tip temperature should be equalized
to that of the solution to prevent contraction or expansion of solution.

Service And Maintenance Information
It is recommended that the following servicing procedures be performed atregular intervals. Heavy usage or usage with corrosive fluids will require more
frequent servicing.

Disassembly (Reference Figure 3 & 4)
1. Turn the screw bolt counterclockwise toremove a tip ejector pipe. Gently pull thetip ejector pipe off the end of tip ejector
pipe during this procedure.
2. Unscrew the barrel assembly from thehandle and carefully pull directly awayfrom the handle. The plunger assemblyand spring can be removed from thepipette. Refer to Figure 4 for the internal
configuration of the pipette being
serviced. Take special care not to bend
the piston rod, especially in the smaller
volume units.
3. Replace the O-ring.

-6-

4. Reassemble the unit by reversing the above procedures. Refer to Figure 4
when reassembling to ensure that all parts are placed in the correct position.
5. To reassemble the tip ejector pipe gently push on the tip ejector pipe so thatthe pipe mates with the handle connection. Turn the screw clockwise tosecure.
6. After the above maintenance procedure, should the feeling of a smooth piston
stroke seem different from what it was before this procedure, immediately
disassemble the pipette as in the above procedure and reassemble per theschematics in Figures 3 and 4.
7. After the above maintenance procedure, should the tip ejector knob stroke
seem different and lack the smoothness of its normal stroke, immediately
disassemble the tip ejector portion of the pipette and reassemble ensuring thespring and spring holder are installed properly.

Fig4-7-Fig.5
NPF2-10, 20

NPF2-25, 30, 50
NPV2-SNPF2-100, 200
NPV2-M

NPF2-500, 1000
NPV2-L

Plunger

1st spring

1st spring
HolderSeal ring
O-RingFilter replacement procedure
NPF2-500,1000,NPV2-L: Fig. 6① Insert the tips of filter remover into two notches on the sides of a filter, andpull out the filter in the direction of the arrow.
② Set the projection of a new filter in the internal groove of the nozzle, and thenpress the filter into the nozzle.
Do not touch filters which may be contaminated by harmful substances.
CAUTION

Fig.6Nozzle filter remover

Nozzle filter-8-Specifications
Cat.No.

Vol. Setting

00-NPF2-10

Fixed

10±1.2≦0.400-NPF2-20

Fixed

20±0.8≦0.300-NPF2-25

Fixed

25±0.8≦0.300-NPF2-30

Fixed

30±0.8≦0.300-NPF2-50

Fixed

50±0.6≦0.300-NPF2-100

Fixed

100±0.6≦0.300-NPF2-200

Fixed

200±0.6≦0.200-NPF2-500

Fixed

500±0.5≦0.200-NPF2-1000

Fixed

1000±0.5≦0.2

10±1.2≦0.600-NPV2-S

Three
variablesetting20±1.0≦0.5

50±0.7≦0.3

50±0.7≦0.4

100±0.6≦0.4

200±0.5≦0.3

200±0.7

≦0.3

500

±0.6

≦0.3

1000

±0.5

≦0.200-NPV2-M00-NPV2-L

ThreevariablesettingThreevariablesetting

Volume (μL) Accuracy (%) Precision (%)

The technical figures given in the Table-1 “Nichipet FII VII Maximum Permissible Errors” wereobtained using genuine Nichiryo BMT Tips.
Nichiryo declares that pipettes comply with the requirement of the ISO 8655 Standard, by typetesting. The adjustment is carried out under strictly defined and monitored conditions (ISO8655-6):●The basis of adjustment, EX●Reference temperature, 20°C-25°C●Relative humidity, more than 50%●Barometric pressure, 101KPa,●Use of distilled water, distilled water-9-Ordering Information
Nichipet-FⅡ Fixed Volume Pipette
Cat.No.

Volume(μL)

Accessories

00-NPF2-10(10μL)
to00-NPF2-1000(100μL)

10,20,25,30,50
100,200,500,1000

Appropriate tips

Nichipet-VⅡ TRIPLE VOLUME
Cat.No.

Volume(μL)

00-NPV2-S10/20/5000-NPV2-M

50/100/20000-NPV2-L

200/500/1000

Accessories
Appropriate tips

Tip (Autoclavable)
Cat.No.

Volume(μL)

Color

Usable Model

Q’ty(bag)

00-BMT2-SS

0.5～10NaturalNPF2-101000pcs

00-BMT2-SG

2～200

Natural00-BMT2-LG

100～1000NaturalNPF2-20～200
NPV2-S,M
NPF2-500,1000
NPV2-L

1000pcs
1000pcs

Racked Tip (Autoclavable)
Cat.No.

Volume(μL)

Color

Usable Model

00-BMT2-SSR

0.5～10NaturalNPF2-1000-BMT2-SGR

2～200

Natural00-BMT2-LGR

100～1000NaturalStand
Cat.No.
00-BMT-STD(Acryl resin)

Description
6 pipttes can bestored.

- 10 -

NPF2-20～200
NPV2-S,M
NPF2-500,1000
NPV2-LQ’ty(box)
960pcs(96pcs.x10c/s)

960pcs(96pcs.x10c/s)

960pcs(96pcs.x10c/s)

Troubleshooting
Symptom

Possible cause

Tip cannot beejected.
Nozzle cylinder loosens

Pipette fails toaspirate liquid.

Seal ring and O-ring areassembled in reverseorder.

Seal ring or/and O-ring set
are worn.
Nozzle cylinder loosens

Extracted
liquid leaks
from tip.

Nozzle cylinder is worn(stepped wear can bechecked by eye).
Seal ring and O-ring set isworn because the plunger
is damaged or rusty.
The tip is loosely attached.

Push button
moves stiffly.

CAUTION

The tip is loosely
attached.

RemedyTighten up the nozzle
cylinder securely.

Reset the seal ring andO-ring set according to
the manual in“Disassembling/reassembling”.

Replace an seal ring andO-ring set.
Tighten up the nozzle
cylinder securely.
Replace an nozzle cylinder.
Replace an seal ring andO-ring set.
Replace an seal ring andO-ring set.
If the push button does
not work well after
dispensing, or it issticking to the body,disassemble the pipette
and wash/clean every
part (or wipe down every
part with soft cloth).
If there are some parts
getting rusty or corroded
inside the body, replace
the parts with new ones.

If the pipette can not be fixed after examining and conducting the above
mentioned procedure, immediately stop using the pipette and ask us or ouragent to repair it.
Before bring the pipette for repair, be sure to check whether it has been
contaminated with microbes or harmful substance.

- 11 -

Memo- 12 -Memo

- 13 -

Memo- 14 -For repair, service or information you may contact your local distributor.

MANUFACTURER:

Tokyo office
1-10-1 Kandanishiki-cho, Chiyoda-ku, Tokyo 101-0054, Japan
TEL: +81-3-6273-7652(English) FAX: +81-3-6273-7944
URL: http://www.nichiryo.co.jp/E-mail: i-sales@nichiryo.co.jp
2016.Ver.1-0-