JST-CREST "Opt Bio" /WPI-IIIS Joint Symposium
Deciphering the Brain through "Opt Bio" Tools

Date

:

Venue

: 2F Hall, Multimedia Education and Research Complex,
Kawauchi-Kita Campus, Tohoku University

Friday, March 18, 2022, 8:30 – 16:00

Time Table

Time Table
Opening |8:30 - 8:45

MC: Mayumi Kimura (WPI-IIIS, University of Tsukuba)
Welcome

Masashi Yanagisawa

Director, WPI-IIIS, University of Tsukuba

Opening Address 1

Akira Ukawa

WPI Program Director

Opening Address 2

Ryoichiro Kageyama

Research Supervisor, CREST Optbio
Director, Center for Brain Science, RIKEN

Session 1 | Chair: Arisa Hirano (WPI-IIIS, University of Tsukuba)
8:45 - 9:15

Computations in neuron-glia circuits forcontrolling behavioral states

Misha Ahrens

HHMI, Janelia Research Campus

Page 4

9:15 - 9:45

Optical tools for studying the brain

Adam E. Cohen

Harvard University

Page 5

9:45 - 10:15

Controlling the fate and function of proteins
with proximity photopharmacology

Dirk Trauner

New York University

Page 6

10:15 - 10:30 BreakSession 2 | Chair: Masashi Yanagisawa (WPI-IIIS, University of Tsukuba)
10:30 - 11:15

Inner workings of channelrhodopsins andbrainsKarl Deisseroth

HHMI / Stanford University

Page 7

11:15 - 11:45

Mechanical interactions of dendritic-spine
synapsesHaruo Kasai

WPI-IRCN, The University of Tokyo

Page 8

11:45 - 12:15

How synaptic plasticity mediates learning andMichisuke Yuzaki
memory in vivo: an optogenetic approach

Keio University School of Medicine

Page 912:15 - 12:25 Photo / Break

12:25 - 13:20 Lunch Break (Bee ARENA Café)

Poster Session | Chair: Kaspar Vogt (WPI-IIIS, University of Tsukuba)
13:20 - 13:50 Data Blitz (2F Hall, Multimedia Education and Research Complex)

Page 14-15

13:50 - 14:30 Poster Presentation (1F, Multimedia Education and Research Complex)

Page 14-15

Session 3 | Chair: Sakiko Honjoh (WPI-IIIS, University of Tsukuba)
14:30 - 15:00

Induction of hypometabolic and hypothermic
states in mice

Spying on neuromodulation by constructing
15:00 - 15:30 a toolbox of genetically encoded fluorescentsensors

Takeshi Sakurai

WPI-IIIS, University of Tsukuba

Page 10

Yulong Li

Peking University

Page 11

University of Bern

Page 12

15:30 - 16:00

Neural circuits underlying sleep structure andAntoine Adamantidis
functions

16:00

Closing2

Information
TOHOKU UNIVERSITY Kawauchi-Kita Campus

JST-CREST "Opt Bio"/
WPI-IIIS Joint Symposium Venue

Guide Map

A05 Multimedia Education Research Complex

Subway Tozai line
Kawauchi Station

Lunch break @ Bee Arena Café
B05

Kawauchi Sub Arena

Subway Tozai line
International Center Station

The 99th Annual Meeting of
the Physiological Society of Japan Venue
A04
A03
A02

Lecture Rooms C
Lecture Rooms B
Lecture Rooms A

Floor Map (1F & 2F) | Multimedia Education Research Complex
M201

WC menM202

WC women

Anteroom
M205

2F

Elevator

M206

M204

M203

Multimedia Hall

Stage

Anteroom

Symposium Venue
WC men

M103

M101

1F

WC women

Poster #35~#50
Elevator

M104

M105

Poster #18~#34
Poster #1 ~#17

M102

Entrance Hall
Reception

Poster Session Venue

3

Session 1

8:45 - 9:15

Computations in neuron-glia circuits
for controlling behavioral states
ABSTRACTNo Abstract

Misha Ahrens
HHMI, Janelia Research Campus,
USA

4

Session 1

9:15 - 9:45

Optical tools for studying the brain
ABSTRACT
Optical tools for simultaneous perturbation andmeasurement of membrane potential enable spatiallyresolved mapping of neural activity with high resolution
in space and time, in behaving animals. I will describe
some advances in voltage indicators, microscope systems,
and analysis software. With these advanced tools, weare studying the dynamics of microcircuits involved incontrol of attention and the sub-cellular details of dendritic
integration. I will also describe some new approaches tostoring brain-wide records of neural activity via intracellular
protein "ticker tapes".

Adam E. Cohen
Harvard University, USA5

Session 1

9:45 - 10:15

Controlling the fate and function of proteins withproximity photopharmacology
ABSTRACT
Photopharmacology endeavors to control biological
function with synthetic photoswitches that can be
attached covalently or non-covalently to their targets- or nearby. I will discuss potential applications ofphotopharmacology in biology and medicine, in particularwith respect to controlling signal transduction and targeted
protein degradation. I will make a case that "Proximity
Photopharmacology" is a particularly effective strategy.

Dirk Trauner
New York University, USA6

Session 2

10:30 - 11:15

Inner workings of channelrhodopsinsand brains
ABSTRACTNo abstract

Karl Deisseroth
HHMI / Stanford University, USA7

Session 2

11:15 - 11:45

Mechanical interactions of dendritic-spine
synapses
ABSTRACT

Haruo Kasai
WPI-IRCN,
The University of Tokyo, Japan

8

The majority of excitatory glutamatergic synapses are madeon dendritic spines which enlarge during learning. Since
dendritic spines and the presynaptic terminals are tightly
connected with the synaptic cleft, the enlargement may
have mechanical effects on presynaptic functions. We foundthat the fine and transient pushing of the boutons by a glass
pipette markedly promoted an evoked neurotransmitter
release and the assembly of soluble N-ethylmaleimidesensitive factor attachment protein receptor (SNARE)
proteins, whose Förster resonance transfer (FRET) was
measured with fluorescence lifetime imaging (FLIM) in
rat slice culture preparations. Surprisingly, both effects
persisted over 20 min. The increased presynaptic FRET was
independent of cytosolic calcium (Ca2+), but dependent on
the assembly of SNARE proteins and actin polymerisationin the boutons. Importantly, a low hypertonic sucrose
solution (20 mM) caused facilitatory effects on both the
FRET and evoked release without inducing spontaneous
release, making a striking contrast with a high hypertonic
sucrose solution (300 mM) which induced exocytosis byitself. Finally, the spine enlargement, induced by the twophoton glutamate uncaging, enhanced evoked release and
FRET only when the spines pushed the boutons by theirelongation. Thus, we have found a mechano-sensory andtransduction mechanism in the presynaptic boutons.

Session 2

11:45 - 12:15

How synaptic plasticity mediates learning andmemory in vivo: an optogenetic approach
ABSTRACT

Michisuke Yuzaki
Keio University
School of Medicine, Japan

Long-term potentiation (LTP) and long-term depression(LTD) of excitatory neurotransmission has been proposed
as a cellular substrate for learning and memory in vivo.
Although LTP and LTD are widespread phenomena
expressed at every excitatory synapse in the mammalianbrain, it is not completely understood whether and how
LTP/LTD at specific synapses are causally linked to learningand memory in vivo. This is mainly because it is unknown
whether LTP/LTD are induced in vivo in similar stimulus
conditions that are used for the induction of LTP/LTD inacute slice preparations. Further, genetic engineering inmice could induce compensatory mechanisms that could
modify synaptic plasticity in the remaining circuits. To
circumvent these problems, we developed new optogenetic
tools, termed PhotonSABER and LysopH-up, which enabled
the temporal, spatial, and cell type-specific inhibitionof LTD and LTP, respectively, while the basal synaptic
properties and other forms of synaptic plasticity were
unaffected. Using these tools at parallel fiber-Purkinje cell
synapses in the cerebellum, we will show how LTD/LTP
at these synapses are causally linked to the cerebellumdependent oculomotor learning in vivo.

9Session 3

14:30 - 15:00

Induction of hypometabolic and hypothermic
states in mice
ABSTRACT

Takeshi Sakurai
WPI-IIIS,
University of Tsukuba, Japan10

We found that chemogenetic/optogenetic excitation of Qrfpexpressing neurons (Q neurons) in a region of the mouse
hypothalamus (anterior ventral periventricular nucleus)
induces sustained hypothermia and hypometabolism, which
we named QIH. The QIH was accompanied by a significant
decrease in body temperature and oxygen consumption
rate. A battery of behavioral tests was performed on the
QIH-experienced and QIH-naïve groups, but no differenceswere found between the two groups, nor were there any
differences in histological observations of the brain, heart,muscles, or other organs. The fact that QIH can be repeatedin the same individual suggests that QIH is a reversible andsafe hypometabolic state, i.e., a hypometabolic state similar
to hibernation. Histological and optogenetic analyses
suggested that Q neurons induce QIH mainly through theDMH. QRFP is widely conserved in mammals, suggestingthat Q neurons may be involved in a hypometabolisminducible neural pathway that is widely conserved in
mammals. Physiologically, Q neurons may be involved in
rapid shifts in body temperature set points and be involved
in circadian control of body temperature. Animals inhibernation are in a state of hypothermia, hypometabolism,
and low activity, but even under these conditions, they canadapt to changes in the environment and spontaneously
return to their original state without any tissue damage. If
the oxygen demand of animals could be safely lowered asin hibernating animals, various applications are possible,
and I would like to discuss the medical application of QIH.

Session 3

15:00 - 15:30

Spying on neuromodulation by constructing a
toolbox of genetically encoded fluorescent sensors
ABSTRACT

Yulong Li
Peking University, China

Diverse neuromodulators in the brain, such as acetylcholine,
monoamines, lipids and neuropeptides, play importantroles in a plethora of physiological processes including
reward, movement, attention, sleep, learning and memory.
Dysfunction of the neuromodulatory system is associatedwith a range of diseases, such as epilepsy, addition,neurodegenerative and psychiatric diseases. A longstanding
yet largely unmet goal is to measure the dynamics ofdifferent neuromodulators reliably and specifically with highspatiotemporal resolution, particularly in behaving animals.
To achieve this goal, we develop a series of geneticallyencoded GPCR-activation-based (GRAB) sensors for thedetection of acetylcholine, dopamine, norepinephrine,
adenosine, ATP, serotonin, histamine, endocannabinoids
and neuropeptides, and validate the performance of thesesensors in multiple preparations in vitro and in vivo.
The GRAB sensor toolbox provides new insights into the
dynamics and mechanism of neuromodulatory signaling
both in health and disease.

11

Session 3

15:30 - 16:00

Neural circuits underlying sleep structure andfunctions
ABSTRACTThe activity of multiple brain circuits is strongly modulated
during sleep states. Some of these are implicated in thetemporal control of the sleep-wake cycle, while others
support sleep-dependent functions including memory
consolidation. In this lecture, I will summarize our recent
work investigating a role for REM sleep in modulating
cellular dynamics of neural circuits controlling of goaloriented behaviours and its implication for the maintenanceof innate behaviour.

Antoine Adamantidis
University of Bern, Switzerland

12

Poster Session
JST-CREST “Opt Bio” / WPI-IIIS Joint Symposium
Deciphering the Brain through “Opt Bio” Tools

Data Blitz by Poster Presenters

13:10 – 13:40

Poster Presentation

13:40 – 14:30

Poster List
Poster list#

Presenter

Title

1

Tsuyoshi Saito

Development of photocaged adenosine 2A receptor activator

2

Koustav Roy

Opto-chemical control of sleep in the nucleus accumbens using a photocaged adenosine A2A
receptor allosteric modulator

3

Hikari Yashiki

Chemogenetic suppression of histamine receptor cells produces slow-waves in mice

4

Iyo Koyanagi

Mechanisms of hippocampal adult-born neurons for memory consolidation during sleep

5

Jiahui Yu

Adult-born neuron activity in the establishment of fear generalization

6

Yuteng Wang

Synchronous young and matured neuron activity for memory consolidation

7

Pablo Vergara

CaliAli: A tool for inter-session alignment of 1-photon calcium imaging data allowing tracking
neurons in the non-rigidly moving brain

8

Sakthivel Srinivasan

Transient recruitment of an adult-born neuron ensemble for fear memory consolidation in REM
sleep

9

Shingo Soya

Whole brain mapping and manipulation of activated neuronal populations by exhausted exercise

10

Saki Yamada

Capturing and manipulating neurons activated by exhausted exercise

11

Emi Hasegawa

Rapid eye movement sleep is initiated by dopamine signaling in the basolateral amygdala in mice

12

Jeong SolGABAergic neurons around ventrolateral periaqueductal gray regulate cataplexy in narcolepsy
model mice

13

Yuki Saito

Visualizing input-output architecture of orexin neurons with double-color projection-selective
retrograde tracin

14

Kseniia Prokofeva

Delineation of neural circuits of galaninergic neurons in the VLPO implicated in regulation of sleep

15

Zhongwen Zhang

Mechanism by which the BNST→DpMe pathway induces arousal

16

Ruth Li

Gastrin-releasing peptide neurons in the suprachiasmatic nucleus play an essential role inregulating circadian rhythm

17

Toru Takahashi

Induction of mouse hibernation-like state using high sensitive optogenetics

18

Yoan Cherasse

Roles of monoamines and their regulatory systems in motivation and arousal

19

Katsuyasu Sakurai

The anatomical and functional understanding of neural dynamics of VTA dopaminergic neurons infemale sexual behavior

20

Ai Miyasaka

Dopamine dynamics in NAc-vs control male sexual behaviors in mice

21

Hibiki Okamura

Analyses of the effect of prior social stress on brain activity during social interaction in mice using
a novel semi-automated c-Fos mapping program

22

Shinnosuke Yasugaki

Is increased REM sleep an adaptive response or an exacerbating factor in stress resilience?

23

Yoshifumi Arai

Characterization of the neuronal activity of a newly identified REM sleep-regulating neuron using
glass pipette extracellular unit recording combined with optogenetics

24

Ami Kaneko

Analysis of the mechanism of REM sleep behavior disorder with focus on Parkinson’s disease

25

Ayako Imamura

Effects of chronic cell ablation in the preoptic area on sleep-wake cycles

26

Yusuke Murakami

Toward label-free molecular imaging of whole brain and brain cells using ultra-broadband
multiplex CARS microspectroscopy

27

Olga Malyshevskaya

The role of hypothalamic supraoptic nucleus in maintaining wakefulness

28

Javier Diaz Cisternas

Recovering EEG generators from their collective stochastic interference

29

GoEun (Abby) Han

Dihydropyridine calcium blockers do not interefere with slow wave sleep

30

Satomi Okabe

The effects of olfactory stimulation during REM sleep on dream emotionality: a study focusing on
individual difference in olfactory perception

14

#

Presenter

Title

31

Tsuyoshi Nemoto

Recovery from systemic-inflammation-induced altered sleep is potentiated by sevofluranepreconditioning

32

Shigeru Chiba

Validation experiment using in-home sleep electroencephalography and its clinical application

33

Juan Carlos Neira
Almanza

Automatic assessment of sleep recordings from a portable IoT EEG device.

34

Haruka Suzuki

Metabolomic and pharmacologic analyses of brain substances associated with sleep pressure in
mice

35

Tomohiro Kitazono

The novel intracellular signaling pathways for the regulation of homeostatic sleep need

36

Liqin Cao

Natural history study of sleep disturbances in CDKL5 deficiency disorder mice

37

Hikari Yamamoto

OX2R-selective orexin agonism is sufficient to suppress narcoleptic symptoms, cataplexy andwake fragmentation, without inducing drug-seeking behavior in mouse models

38

Mahesh Kaushik
Kumar

Pre-narcoleptics are more prone to suvorexant-induced cataplexy39

Mari Hondo

Orexin receptor antagonists ameliorate the symptoms of REM sleep behavior disorder in a novel
mouse model and in human patients40

Deependra Kumar

Learning and memory deficit in adult dreamless mice41

Tomoyuki Fujiyama

NALCN in the forebrain and pons-medulla regions have distinct roles in REM sleep regulation

42

Asmaa Elhosainy
Mohamed Mahmoud

Sleepy mouse as a model of idiopathic hypersomnia

43

Yuki TairaElectrophysiological analysis of ion channel mutations in mice with REM sleep abnormalities

44

Noriko Hotta

Sleep/wakefulness and body weight growth from infancy to adulthood in a hypersomnia model,
Sleepy mutant mouse.

45

Patricia Seoane
Collazo

SIK3 in different hypothalamic areas mediates whole-body energy balance

46

Kanako Iwasaki

Where does Sleepy mutation of SIK3 cause sleep phenotypes?

47

Fuyuki Asano

Sik3 regulates sleep need via glutamatergic neurons in cerebral cortex

48

Shinya Nakata

Molecular mechanisms for SIK3(Sleepy)-mediated sleep/wake regulation

49

Minjeong Park

Sleep/wake behavior of mice lacking PKA phosphorylation site in SIK350

Kim Staci Jakyong

Loss of canonical Hdac4 signaling leads to disregulated NREMS

15

MEMO