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Solon, Ohio 44139

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TECHNICAL INFORMATION
Catalog Number: 1050120, 1050122, 1050124
Williams Medium E, powder, with L-glutamine, without sodium bicarbonate
Description: Isolated epithelial cells, as described in his sequential plating method by Williams, et al., in 1971, were originallycultivated in a rich medium known as Williams' Medium D. From these early newborn animal studies, Williams and Gunndeveloped a subsequent medium, Williams' Medium E, for the long-term cultivation of adult rat liver epithelial cells.
Instructions for Use: Mix powder with water at about 90% of volume indicated on the label (1l or 10L). Add sodium bicarbonate
as either powder or liquid in the amounts indicated below. the pH prior to the addition of sodium bicarbonate is approximately
3.8 and after the addition will be in the range of 6.8 - 7.3. Sterile filter, as desired.
Formulation:
Component

mg/lt

Mol. Wt.

Mol. (mM)

90.00000

89.1

1.01

60.50000

174.20.35

20.00000

150.1

0.13

30.00000

133.1

0.23

58.00000

175.6

0.33

26.09000

313.2

0.08

50.00000

147.1

0.34

292.00000

146.1

2.00

50.00000

75.07

0.67

20.30000

209.6

0.10

50.00000

131.2

0.38

75.00000

131.2

0.57

87.50000

182.6

0.48

15.00000

149.2

0.10

25.00000

165.2

0.15

30.00000

115.1

0.26

10.00000

105.1

0.10

40.00000

119.1

0.34

Amino Acids
L-Alanine
L-Arginine HCl
L-Asparagine H2O
L-Aspartic Acid
L-Cysteine HCl H2O
L-Cystine 2HCl
L-Glutamic Acid
L-Glutamine
Glycine
L-Histidine HCl H2O
L-IsoleucineL-Leucine
L-Lysine HCl
L-Methionine
L-Phenylalanine
L-Proline
L-Serine
L-Threonine

L-Tryptophan
L-Tyrosine
L-Valine

10.00000

204.2

0.05

35.00000

181.2

0.19

50.00000

117.1

0.43

0.50000

244.3

0.00201.00000

396.7

0.00251.50000

139.6

0.01071.00000

238.3

0.00420.01000

554.7

0.000021.00000

441.4

0.00232.00000

176.1

0.01140.01000

330.2

0.000032.00000

180.2

0.01111.00000

122.130.00821.00000

203.6

0.00490.10000

376.4

0.00031.00000

337.3

0.00300.10000

328.5

0.00030.20000

1355.40.0001

264.90000

147

1.80

0.00006

159.680.0000004

0.0001404

0.00000025

97.70000

120.4

0.81

0.00010197.9

0.0000005

400.00000

74.55

5.37

6800.00000

58.44

116.36140.00000

159.960.88

0.00020287.5

0.0000007

2000.00000

180.2

11.10

0.05000

307.330.0002

Vitamins
BiotinCalciferol
Choline Chloride
D-Calcium Pantothenate
DL-a-Tocopherol PO4 Na2
Folic Acid
L-Ascorbic Acid
Menadione Sodium Bisulfite 3H2O
myo-Inositol
Nicotinamide
Pyridoxal HCl
Riboflavin
Thiamine HCl
Vitamin A Acetate
Vitamin B12
Inorganic Salts
Calcium Chloride [CaCl2 2H2O] Dihydrate
Cupric Sulfate [CuSO4]
Ferric Nitrate [Fe(NO3)3 9H2O] Nonahydrate
Magnesium Sulfate [MgSO4]
Manganese Chloride [MnCl2 4H2O] Tetrahydrate
Potassium Chloride [KCl]Sodium Chloride [NaCl]
Sodium Phosphate Monobasic [NaH2PO4 H2O] Monohydrate
Zinc Sulfate [ZnSO4 7H2O] Heptahydrate
OtherDextrose
Glutathione (reduced)

Methyl Linoleate
Phenol Red Sodium Salt
Sodium Pyruvate

0.03000

294.50.0001

10.00000

376.40.03

25.00000

1100.23

Add either one to adjust pH to 7.4
NaHCO3 Powder (g/L)

2.2000NaHCO3 7.5% Solution (mL/L)

29.4000

Availability:
1X Liquid:
Catalog Number
1250254

Description
1X Williams' Medium E without L-glutamine

Size
500 mlPowder:Catalog Number
1050122

Description
Williams' Medium E with L-glutamine without sodium bicarbonateSize
1 x 10 liter

Reference:– Williams, G.M., Weisburger, E.K. and Weisburger, J.H., J. Exptl. Cell Res., v. 69, 106 (1971).