DATA SHEET
20221028

Anti-VGluT1(vesicular glutamate transporte-1)
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Species :Product Code :RRID :MSFR106180
VGluT1 pAb (Rb) 20ug
20μg (affinity-purified with antigen polypeptide)
RabbitVGluT1-Rb-Af500
AB_2571616
MSFR106190
VGluT1 pAb (Rb) 50ug
50μg (affinity-purified with antigen polypeptide)
RabbitVGluT1-Rb-Af500
AB_2571616
MSFR106200
VGluT1 pAb (Go) 20ug
20μg (affinity-purified with antigen polypeptide)
Goat
VGluT1-Go-Af310
AB_2571617
MSFR106210
VGluT1 pAb (Go) 50ug
50μg (affinity-purified with antigen polypeptide)
Goat
VGluT1-Go-Af310
AB_2571617
MSFR107430
VGluT1 pAb (GP) 20ug
20μg (affinity-purified with antigen polypeptide)
Guinua pig
VGluT1-GP-Af1650
AB_2571618
MSFR107440
VGluT1 pAb (GP) 50ug
50μg (affinity-purified with antigen polypeptide)
Guinua pig
VGluT1-GP-Af1650
AB_2571618

Formulation : Liquid ; 200 g/ml in PBS with 0.05% NaN3.
Storage : Store at cool temp (2-10˚C).
The antibody can be stored at 2-10˚C.
for long-term storage.

The antibody can be also aliquoted and stored at -80˚C

Avoid repeated freeze-thawing. Non-hazardous.

No MSDS required.

Species : rabbit / guinea pig / goat , polyclonal
Antigen : Mouse VGluT1, C-terminal 531-560 aa (BC054462)
ご注意：本商品には 0.1 % 未満のアジ化ナトリウムが入っています。
大量の水で洗い流してください。

Distributor : NITTOBO MEDICAL CO., LTD
TEL : +81-(0)3-4582-5451
email : nmd-tokuhan@nittobogrp.com

誤って目や口に入ったり、皮膚に付着した場合はManufacture : FRONTIER INSTITUTE CO., LTD

DATA SHEET
20221028

Specificity : mouse (others not tested)
Immunoblot detects a single protein band at 60 kDa.
This selectively stains distinct populations of glutamatergic neurons, particularly theirterminals. This is also useful marker to visualize parallel fiber terminals innervating Purkinjecells and corticostriatal afferent terminals.

Applications : In general, affinity-purified antibody is used at around 1 microgram/ml for
immunoblot and immunohistochemistry. The most appropriate concentration should bedetermined by users, because it depends on contents in given cells, tissues and organs.
Research Use : For research use only, not for use in diagnostic procedures.
Remarks : Rabbit and guinea pig antibodies are similar in the titer and specificity.
Notice : When using goat VGluT1 antibody for multiple immunofluorescence, mixture ofprimary antibodies is better to be avoided. By unknown reason, secondary antibodies produced
in non-goat species happen to cross-react to goat VGluT1 antibody in some, not all, cases.
Step-wise method in multiple immunofluorescence can overcome this problem. Namely,immunoreaction with goat VGluT1 antibody and detection with fluorophore-conjugatedanti-goat IgG is performed first, followed by the second round of immunoreaction with otherprimary antibodies.
Reference : 1) Miyazaki, T., Fukaya, M., Shimizu, H, Watanabe, M. (2003) Developmental
switching of vesicular glutamate transporters at parallel fiber-Purkinje cell synapses. Eur. J.
Neurosci 17:2563-2572.
2) Kawamura, Y., Fukaya, M., Maejima, T., Yoshida, T., Miura, E., Watanabe, M.,
Ohno-Shosaku, T., Kano, M. (2006) CB1 is the major cannabinoid receptor at excitatory
presynaptic site in the hippocampus and cerebellum. J. Neurosci., 26:2991-3001.

3) Miura, E., Fukaya, M., Sato, T., Sugihara, K., Asano, M., Yoshioka, K., Watanabe, M.
(2006) Expression and distribution of JNK/SAPK-associated scaffold protein JSAP1 indeveloping and adult mouse brain. J. Neurochem. 97:1431-1446.

ご注意：本商品には 0.1 % 未満のアジ化ナトリウムが入っています。
大量の水で洗い流してください。

Distributor : NITTOBO MEDICAL CO., LTD
TEL : +81-(0)3-4582-5451
email : nmd-tokuhan@nittobogrp.com

誤って目や口に入ったり、皮膚に付着した場合はManufacture : FRONTIER INSTITUTE CO., LTD