各種シンポジウム

第 35 回生理研コンファレンス・統合脳国際シンポジウム
Recent Advances in Cortical and Hippocampal Microcircuits
大脳皮質・海馬の局所神経回路研究第 35 回生理研コンファレンス・統合脳国際シンポジウム「大脳皮質・海馬の局所神経回路研究」は，2006 年 7 月
24 日−26 日の 3 日間に，自然科学研究機構・岡崎コンファレンスセンターにおいて開催された。大脳皮質の神経回路構築の解析は，現在，大きな飛躍の時を迎えていると言っても過言ではない。今まで未知であった多くの事実が分子脳科学や多電極記録法等のシンポは目覚ましく，多くの新しい事実が次々と報告されている。その中でも，リーディング的存在の研究者達（Edward M Callaway 博士 (Salk Institute, USA), Sacha B Nelson 博士 (Brandis Univ., USA), Gabor
Tamas 博士 (Szeged Univ., Hungary), Jackie Schiller 博士 (Technion, Israel), Takao K Hensch 博士 (RIKEN-BSI, HarvardUniv. USA)）にご参加頂き，最新の成果を発表していただいた。さらに，日本の大脳皮質神経回路研究の諸先生方にも，秀でた研究を発表していただき，トータルで 18 演題の口頭発表について活発な質疑応答が交わされた。さらに，ポスター発表にも 31 演題が集まり，日本の若き神経科学研究者や院生達も，第一線を走っておられる研究者と直接ディスカッションする機会を得た事で，大いに刺激を受けた事であろう。総数 124 名（うち，外国人 14 名）の参加者同士の交流も深まり，多くの意味で非常に有意義であった。打ち解けた雰囲気の中で実り多いディスカッションが行われ，非常に楽しい会合となった。

Monday, July 24
Opening Remarks Yumiko Yoshimura
Session 1: Physiological network connectivity in hippocampal microcircuitry
1. Yuji Ikegaya (Univ. of Tokyo, Tokyo)
Spontaneous activity and ongoing plasticity of CA3 recurrent networks459

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2. Adi Cymerblit and Yitzhak Schiller (Technion, Israel)
Network dynamics during the development and maintenance of seizures in pilocarpine and picrotoxin treated rats
Session 2: Anatomical network connectivity in hippocampal microcircuitry
3. Ryuichi Shigemoto (NIPS, Okazaki)
Asymmetrical organization of glutamate receptors in left and right hippocampal synapses
4. Shozo Jinno, Thomas Klausberger, Laszlo F. Marton, Yannis Dalezios, Pablo Fuentealba, Wai Yee Suen, Eric A. Bushong,
Darrell Henze, György Buzsáki and Peter Somogyi (Oxford Univ., UK: Hungarian University of Transylvania, Hungary:
University of Crete, Greece: Institute of Applied and Computational Mathematics. Greece: The State University of NewJersey, USA)
Novel long-range GABAergic projections in the hippocampal formation and beyond
Session 3: Network connectivity in cortical microcircuitry
5. Gábor Tamás, János Szabadics, Szabolcs Oláh, Gergely Komlósi, Pál Barzó, Gábor Molnár (Univ. of Seged, Hungary)Single spike triggered event sequences in networks of the human cerebral cortex in vitro
6. Yasuo Kawaguchi (NIPS, Okazaki)
Layer V pyramidal cell diversity and their synaptic connections
Tuesday, July 25
Session 4: Plasticity in the visual cortex
7. Yumiko Yoshimura (Nagoya Univ., Nagoya)
Functional roles of T-type Ca2+ channels in visual cortical plasticity
8. Takao K Hensch (RIKEN-BSI, Wako)
GABA circuit control of critical period plasticity in visual cortex
Session 5: Microcircuitry in vivo
9. Victoria M Puig, Mika Ushimaru, Yoshiyuki Kubota, Akiya Watakabe, Tetsuo Yamamori, Yuchio Yanagawa, Yasuo Kawaguchi(NIPS & NIBB, Okazaki, Gunma Univ., MIT, USA)
Cortico-striatal, cortico-raphe and fast-spiking cell activity in the rat frontal cortex during cortical oscillations in vivo:
modulation by serotonin
10. Ichiro Fujita (Osaka Univ., Osaka)
Functional and Anatomical Architecture of the Inferior Temporal Cortex
Session 6: Microcircuitry of inhibitory neurons
11. Edward Callaway, Takuma Mori, Xiangmin Xu, Ian Wickersham, and David Lyon (Salk Inst, USA)
Unraveling cell type and fine-scale specificity of cortical connections
12. Yukio Komatsu (Nagoya Univ., Nagoya)A new form of inhibitory synaptic actions between nearby pyramidal neurons in visual cortex
13. Fumitaka Kimura (Osaka Univ., Osaka)
Cholinergic control of cortical network and thalamocortical transmission

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Wednesday, July 26
Session 7: Anatomical aspects of cortical microcircuitry
14. Kathleen Rockland (RIKEN-BSI, Wako)
Cortical projection neurons: phenotypes and input maps
15. Takaichi Fukuda (Kyushu Univ, Fukuoka)
Dendritic network of cortical interneurons linked by gap junctions
Session 8: Synaptic integration along dendrite
16. Jackie Schiller (Technion, israel)
Synaptic integration and plasticity within non-linear dendritic subunits

Session 9: New aspects in cortical microcircuitry analysis
17. Yoshiyuki Kubota, Fuyuki Karube, Yasuo Kawaguchi (NIPS, Okazaki)
Dendritic dimensions of cortical GABAergic nonpyramidal cells
18. Sacha B Nelson (Brandeis Univ., USA)
Physiological Genomics of Cortical Circuits in Health and Disease
Closing Remarks

Yoshiyuki Kubota

PosterPoster I

Anatomical and Physiological Analysis of Local Circuits in Neocortex

P-1. Quantification of excitatory and inhibitory synapses onto parvalbumin- and calretinin-positive GABAergic neurons in therat cerebral cortex.
Akio Sekigawa, Yoshiyuki Kubota, Yasuo Kawaguchi
Div. Cerebral Circuitry, NIPS, Okazaki, Dept. Physiol. Sci. Grad. Univ. Advanced Studies, OkazakiP-2. Cortical interneuron organization analysis by in vivo Venus labeling of GABAergic cells in BAC transgenic rats
Yasuharu Hirai1,2, Masakazu Uematsu2,3, Satoe Ebihara2, Kuniya Abe4, Sachiko Yosida3, Megumi Kato2, Masumi
Hirabayashi2, Yuchio Yanagawa5,6 and Yasuo Kawaguchi1,21Grad. Univ. Advanced Studies, Okazaki, Japan, 2NIPS, Okazaki, Japan, 3Toyohashi Univ. Tech., Toyohashi, Japan,

4

RIKEN, Tsukuba, Japan, 5Gunma Univ., Gunma, Japan, 6SORST

P-3. Regional, cell type, and layer-specific differences in cholinergic inhibition of neocortical neurons
Allan T. Gulledge1,2, Susannah Park2, Greg J. Stuart2 & Yasuo Kawaguchi1,1Division of Cerebral Circuitry, National Institute for Physiological Sciences, Okazaki, Japan, 2Divison of Neuroscience,JCSMR, Australian National University, Canberra, Australia
P-4. Laminar sources of synaptic input to layer 1 neurons in rat visual cortex
Takuma Mori, Edward M. Callaway
Systems Neurobiology Laboratories, Salk Inst., La Jolla, CA, USA
P-5. Fluorescence activated cell sorting and expression-profiling of parvalbumin-positive GABAergic neocortical interneurons
from mouse barrel cortex.
Ethan M. Goldberg, Hyo-Young Jeong, and Bernardo Rudy.
NYU School of Medicine, Department of Physiology & Neuroscience, New York, U.S.A.

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P-6. How is γ frequency rhythmic firing of neocortical regular spiking neurons shaped by recurrent inputs?
Kenji Morita1, Rita Kalra2, Kazuyuki Aihara3, and Hugh P. C. Robinson2
RIKEN Brain Science Institute, 2Department of Physiology, Development, and Neuroscience, University of Cambridge,

13

Institute of Industrial Science, University of Tokyo, and ERATO, JST

P-7. Multisite recordings on the signal propagation pattern in local circuit of the visual cortex
Makoto Osanai, Yusuke Takeno, Ryousuke Hasui, Tetsuya Yagi
Graduate School of Engineering, Osaka University
P-8. Synaptic connection patterns between pyramidal cell subtypes in layer V of rat frontal cortexMieko Morishima, Yasuo Kawaguchi
Division of Cerebral Circuitry, National Institute for Physiological SciencesP-9. Target-dependent diversity of intrinsic membrane properties in rat frontal Layer 5 pyramidal cells
Takeshi Otsuka, Mieko Morishima, and Yasuo Kawaguchi
Div. Cerebral Circuitry & Structure, NIPS, Okazaki
P-10. A distinctive layer 5 pyramidal neuron in monkey association cortex, morphologically identified by EGFP – adenovirus
infection
Marie Wintzer, Kathleen S. Rockland
Lab for Cortical Organization and Systematics, RIKEN BSI, Wako, Japan
P-11. Classification of layer 6 neurons by in situ hybridization
Akiya Watakabe1, Noritaka Ichinohe2, Sonoko Ohsawa1, Tsutomu Hashikawa3, Kathleen S. Rockland2, and Tetsuo
Yamamori1
1

Div of Brain Biol., National Institute for Basic Biology, 2Lab for Cortical Organization and Systematics, RIKEN BSI,3

Lab for Neural Architecture, RIKEN BSI

Poster II

Physiological Analysis in vivo

P-12. Burst spikes encode multiple quantities in a time-compressive manner
Toshiyuki Ishii1,2, Tomonori Manabe1 and Toshihiko Hosoya1
RIKEN Brain Science Institute, 2Toho University

1P-13. Direction selectivity and arrangement of neurons in single barrel columns in the rat somatosensory cortex examined with invivo two-photon calcium imaging
1Koji Ikezoe, 2Yoshiya Mori, 3,4Kazuo Kitamura, 2,4Hiroshi Tamura, 2,4Ichiro Fujita

1Grad. Sch. Engineering Science, 2Grad. Sch. Frontier Biosciences, 3Grad. Sch. Medicine, Osaka University, Osaka, Japan,

4CREST, JST, Saitama, Japan

P-14. Spatiotemporal dynamics of surround suppression in cat V1: spatial-frequency dependency
Ayako Ishikawa, Satoshi Shimegi, Hiroyuki Kida, Hiroshi Sakamoto, Hiromichi SatoGrad. Sch. Front. Biosci. & Grad. Sch. Med., Osaka Univ., Japan
P-15. Spatiotemporal dynamics of surround suppression in cat V1: stimulus-size and orientation-contrast
Satoshi Shimegi, Hiroyuki Kida, Ayako Ishikawa, Hiroshi Sakamoto, Hiromichi Sato
Grad. Sch. Med., & Grad. Sch. Front. Biosci., Osaka Univ., Japan
P-16. Clustering of color-selective cells in macaque area V4: analysis with multiple single-unit recordings
Yasuyo Kotake, Hiroshi Morimoto, Hiroshi Tamura, Ichiro Fujita
Grad. Sch. Frontier Biosciences, Grad. Sch. Engineering Science, Osaka Univ., CREST, JST, Japan462

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P-17. Inter-spike interval statistics and visual stimulus discrimination capability of neurons in the monkey inferior temporalcortex
Shunta Tate, Hiroshi Tamura, Ichiro Fujita
Lab Cognitive Neurosci., Grad Sch Frontier Biosciences, Osaka University, Osaka, Japan, Japan Society for the Promotionof Science, Tokyo, Japan
Poster III

Development and Plasticity in Neocortex

P-18. Postnatal changes in the colocalization of VGluT1 and VGluT2 immunoreactivities at single axon terminals of the mouseneocortex
Kouichi Nakamura1,2, Akiya Watakabe3, Hiroyuki Hioki1, Fumino Fujiyama1, Yasuyo Tanaka1, Tetsuo Yamamori3,Takeshi Kaneko1,21

Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, 2CREST, JST, 3Division ofBrain Biology, National Institute for Basic Biology
P-19. Activity-dependent development of interhemispheric connections in mouse visual cortex
Hidenobu Mizuno, Tomoo Hirano, Yoshiaki Tagawa
Dept. Biophys., Kyoto Univ. Grad. Sch. Sci., Kyoto, Japan; CREST, JST, Kawaguchi, Japan
P-20. Transient layer-specific zinc-positive neurons in the developing rat somatosensory cortical system
Noritaka Ichinohe, Daniel Potapov, Kathleen S Rockland
Laboratory for Cortical Organization and Systematics, Brain Science Institute, RIKEN, Wako, Japan
P-21. Layer and area specific gene expression profiling in developing rat cerebral cortex
Toshio Miyashita, Marie Wintzer, *Tomokazu Konishi, Noritaka Ichinohe, Kathleen S. Rockland.
Lab. for Cortical Organization and Systematics, Brain Science Institute, RIKEN. *Faculty of Bioresource Sciences, Akita
Prefectural UniversityP-22. Truncated TrkB-T1 regulates the morphology of neocortical layer I astrocytes in adult rat brain slices
Koji Ohira1,2, Nobuo Funatsu1, Koichi J Homma3, Yoshinori Sahara1, Motoharu Hayashi4, and Shun Nakamura1,21

Department of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology andPsychiatry, Tokyo, Japan. 2Core Research for Evolutional Science and Technology (CREST), Japan Science andTechnology Agency, Saitama, Japan. 3Department of Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo
University, Kanagawa, Japan. 4Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto
University, Aichi, Japan.
P-23. Dynamic role of inhibitory circuits in visual cortical plasticity.
Y. Yazaki-Sugiyama, K. Siu*, T. Fukai*, T. K. Hensch;
Lab for Neuronal Circuit Development, *Neural Circuit Theory, RIKEN BSI, Wako, JAPANPoster IV

New Techniques in Cortical Microcircuitry Analysis

P-24. Efficient Gene Transduction in Neurons with Lentivirus by Improved Neuron-Specific Promoters
Hiroyuki Hioki1,, Hiroshi Kameda1, Hisashi Nakamura1, Taro Okunomiya1, Kohei Ohira1, Kouichi Nakamura1,2, Masako
Kuroda1, Takahiro Furuta1, Takeshi Kaneko1,2
1

Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, Kyoto, Japan. 2Core

Research for Evolutional Science and Technology, Japan Science and Technology Agency (CREST), Kawaguchi, Japan
P-25. Development of dendrite-targeting signals using lentivirus vectors with neuron-specific promoter
Hiroshi Kameda1, Hiroyuki Hioki1, Takahiro Furuta1, Koji Ohira1, Wakoto Matsuda1, Kouichi Nakamura1,2, Takeshi
Kaneko1,2463

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1

Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, Kyoto, Japan, 2CREST,JST
P-26. Golgi-like Visualization of cortical neurons by virus vectors.
Ryohei Tomioka and Kathleen S. Rockland
Lab for Cortical Organization and Systematics, RIKEN BSIP-27. A Novel Genetic Method for Reversibly Inactivating Mammalian Neurons In Vivo
Yoshiaki Yamaguchi1, Elaine M. Tan1,3, Gregory D. Horwitz2, Thomas D. Albright2 and Edward M. Callaway1
1

Systems Neurobiology Laboratories, 2Vision Center Laboratory, The Salk Institute, La Jolla, USA, 3Neurosciences

Graduate Program, UCSD, La Jolla, USA,
Poster V

Physiological Analysis in Hippocampus

P-28. The effects of body temperature on hippocampal neural activity: control of membrane potential through TRPV4 activation
Koji Shibasaki1,2, Makoto Suzuki3, Atsuko Mizuno3, Makoto Tominaga1,21Section of Cell Signaling, Okazaki Institute for Integrative Bioscience, 2Department of Physiological Sciences, The

Graduated University for Advanced Studies, 3Department of Pharmacology, Jichi Medical University
Poster VI

Anatomical Analysis in Basal Ganglia

P-29. Difference in Organization of Corticostriatal and Thalamostriatal Synapses between Patch and Matrix Compartments ofRat Neostriatum.
Fumino Fujiyama1, Tomo Unzai1, Kouichi Nakamura1,3, Sakashi Nomura2, and Takeshi Kaneko1,3
1

Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, 2Department of PhysicalTherapy, School of Health Sciences, Faculty of Medicine, Kyoto University, Kyoto, JAPAN and 3CREST, JST.
P-30. Axonal arborization of midbrain dopaminergic neurons: single-cell study
Wakoto Matsuda1, Takahiro Furuta1, Koichi Nakamura1,2, Takeshi Kaneko1,2
1

Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University2 CREST

P-31. Single cell tracing of thalamostriatal projection neurons with reference to patch and matrix compartments of rat striatum
Tomo Unzai1, Fumino Fujiyama1, Takeshi Kaneko1,2
1

Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, Kyoto, Japan, 2CREST,

Japan

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