BC43
The Ultimate Benchtop
Microscope Family
Key Features

Key Applications

P Benchtop multimodal imaging system
P Instant confocal: Blur-free imaging
P Widefield imaging
P Differential phase contrast & brightfield
P Guaranteed optical performance
P Super resolution readyP In-field upgradable imaging modes

P Cell biology
P Developmental biology
P Neuroscience
P Cancer biology
P Tissue imaging
P Organoids & large organisms
P Microbiology

andor.oxinst.com

Introduction | Applications | Software | Features | Specifications | Ordering

Andor Benchtop Microscope
A Microscopy System that Grows with Your Research

1 Advanced
imaging2

Enhanced visualisation software
Intuitive and powerful. Achieve
outstanding results quickly withminimal training.

design8 Benchtop
Light tight lid and inbuilt

technology
Sharp 2D & 3D
images instantly.

anti-vibration, so no needfor a darkroom or optical
table.

performance
7 Optimal
BC43 is the only

3

confocal microscopewith a publishedperformance
specification and a full
quality control program
to manage its classleading performance.

NEW
Easy super resolution
Push past the diffraction
limit to reveal the innerworkings of the cellto use4 EasyErgonomic joystick and 2x

objective allow quick sample
overview.

2

5

NEW
In-field upgradeable
BC43 provides an upgrade
path from widefield
fluorescence to confocal tosuper resolution.

6 Flexible
Confocal, widefield and transmitted light
imaging modes to suit your experiment.

3

Introduction | Applications | Software | Features | Specifications | Ordering

Andor Benchtop Microscope
Total Imaging Flexibility
 Widefield Imaging

 Confocal Imaging

The most commonly used fluorescence imaging method
and often the tool used by researchers new to microscopy.
Perfect for live sample imaging, low signals and highproductivity. Most compatible with thinner samples andClearView deconvolution can be added to remove haze inthicker samples.

Confocal technology provides high-contrast,
blur-free images. It boosts image quality of thinsamples, such as monolayer cultures, and is
especially suited for thick samples like small
model organisms, 3D cultures and cleared
tissues. BC43 captures images at least 10x
faster than point scanning confocals, boostingproductivity, yet maintaining full resolution. Image
deeper with higher quality than solutions that
rely on computational clearing or deconvolution
alone.

 Transmitted Light Imaging
BC43 offers two transmitted light options: Brightfield forsamples with inherent contrast like larger organisms, andDifferential Phase Contrast (DPC), that can be applied forsamples which deliver high and low contrast.

 ClearViewTM GPU
ClearView GPU powered deconvolution removes thenatural out-of-focus haze from your widefield image for
optimal image quality and resolution. Useful in samples
up to around 50 microns thick (varies with sample
preparation and its optical properties).

Brightfield

Until now confocal has been too expensive andcomplex for many. BC43 is revolutionary – a
confocal at the heart of your lab at an affordable
price with no expertise required!

✓  Super Resolution Imaging
BC43 provides accessible super resolution
imaging - whatever your research discipline.
Push through the diffraction barrier to reveal the
inner workings of the cell. Whether you want tostudy the cytoskeleton in enhanced detail, or gain
greater insights into specific cellular processes,
super resolution is easy to add to your existing
imaging workflow.

Widefield

DPC

BM42 or BC43 WF

BC43 CFBC43 SR

Brightfield - White light transmitted light monochrome imaging
Differential Phase Contrast - High contrast label-free imaging
Widefield epifluorescence - Routine fluorescence imaging
Motorised XYZ - Multipoint large area montages & volume acquisition
Confocal - Deep 3D fluorescence imaging
Super resolution

ConfocalWidefield & ClearViewTM

DPC & Confocal

ClearViewTM - haze removal
Confocal - Deep 3D*
Super resolution

Super resolution
Key
Common Features
Specific Features
Upgrade options*BC43 WF only

4

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Introduction | Applications | Software | Features | Specifications | Ordering

Application Focus
Cell Biology

Developmental Biology

Working closely with leading cell biologists we havecarefully developed BC43 to meet the needs of abroad range of experiments. Reveal the detail inside
cells from nm to mm within tissues and whole modelorganisms with BC43. Use BC43 in confocal mode tosee detail hidden in the sample background or image
in widefield to increase sensitivity and speed.

BC43 cuts through the challenges easily,
spanning development from the first rounds ofcell division to the fully developed organism.
Use BC43 to image at depth, in gentle live
imaging experiments of cells and tissues.
Effortlessly acquire multiple Z stacks, multiple
tiles in combination with time-lapse imaging.

Image fast dynamic events, such as microtubule
dynamics, or study longer processes like cell cycle
over 24 hours with no photobleaching or phototoxicity.

BC43 delivers fast high-resolution imaging ofdeveloping model organisms (e.g. zebrafish anddrosophila). Imaging deeper than conventional
fluorescence microscopes and delivering a
10-fold more productive experience than a
traditional confocal. No sacrificing sensitivity,
resolution or 3D detail for speed, or to avoid
bleaching.

BC43 features for cell biology:
3 Image long processes.
3 Image fast dynamic events.
3 No photobleaching or phototoxicity.
3 nm to mm imaging capability.

Mammalian cell in prophase. Image was acquired
using BC43 confocal mode, using 4 acquisition
channels and covering 10 μm Z range at Nyquist.
Image was further deconvolved and rendered in
Imaris. Dark blue – actin, yellow – microtubules,
magenta - mitochondria, cyan-DNA.
Image credit: Claudia Florindo, Andor Technology.

BC43 features for development biology:
3 Fast high-resolution imaging.
3 Image deep in both live and fixed samples.
3 Montage & seamless stitching at any level ofmagnification.

“I found BC43 super easy to setup
for all my experiments and super
fast to acquire and deliver highquality data. I love its flexibility.”
Zebrafish fin in the process of bone regeneration.
Confocal image shows the perfect stitching of 4
imaging fields, using three channels and 51 stacks
for each field, covering a Z range of 174 μm. Newly
formed bony tissue in purple (calcein staining) andcathepsin k+ cells (the osteoclasts) in yellow, DNA
is in Cyan. Image credit: Alessio Carletti, Universidade
do Algarve.

6

Marco Campinho,Group Leader CBMR- UAlg.

Drosophila egg chamber stained with actin (cyan) andDNA (yellow). The image is a maximum intensity projectionof 309 Z planes acquired covering a range of 93 μm. The
image was further deconvolved in Imaris. Image credits: Rui
Silva, CBMR Universidade do Algarve and Claudia Florindo,
Andor Technology.
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Introduction | Applications | Software | Features | Specifications | Ordering

Application Focus
Tissue Imaging
Large area imaging needs to provide both cellular resolution andthe full organ context. The advanced high-speed technology inBC43 means you no longer need to compromise. Large area tissue
confocal imaging is now possible. Ten times faster than regularconfocals. No sacrifices in resolution, or field of view. BC43 delivers
results fast, shortening the time to publication.
Discover more in intact tissues, use cleared samples and BC43
in confocal mode to image even thicker samples. BC43 takes
advantage of the working distance of modern objectives: imaging
hundreds of microns at high magnifications, and beyond.
BC43 features for tissue imaging:
3 Fast confocal and low light widefield imaging.
3 Seamless large tissue imaging for fixed and live sample.
3 Image from nm to mm.

Zebrafish intestine stitched image. Image was acquired using the confocal imaging modality ofBC43, with 4 imaging channels, 77 stacks and 28 tiles. The full stitched image is composed of a
total of 15,092 images. The deconvolution and stitching options were both activated on the protocol.
Sample courtesy of Julien Resseguier, at NorMic, University of Oslo. Image credit: Claudia Florindo,
Andor Technology.

89Introduction | Applications | Software | Features | Specifications | Ordering

Application Focus
Neuroscience

Cancer

BC43 is the perfect workhorse for neuroscience.
Imaging experiments commonly require highmagnification, for resolution, imaging of large
areas to fully understand the architecture
and connectivity of this complex tissue. The
incredible confocal capture rate of BC43
dramatically reduces imaging time in thicksamples delivering faster results.

BC43 is a push-button confocal suitable for the broadest
range of cancer experimental models. Capturing stunning
images of:• Subcellular events (e.g. cytoskeletal dynamics).
• Intercellular interactions.
• Migration and division.
• 3D cultures from spheroids and organoids.
• Intact tissue and tumour models.

BC43 features for neuroscience:
3 Image both fixed and live samples.
3 Capture deep into cleared brain sections.
3 Developing organoids.
3 Cover the breadth of neuroscience
microscopy needs.
Imaris Quant features for neuroscience:
3 Interact with large 3D images of thicksamples.
3 Easy-to-use animation creation.
3 Calculate overlap of proteins.
3 With additional modules use AI tools to
identify and trace neurons.

Use Imaris to analyse key parameters such as distributionof objects around a surface, volume overlap and nearest
neighbour analysis. Powerful machine learning classification,and batch mode can be used to deliver reproducible resultsin time saving workflows.
Confocal image of mouse embryonic stem cell derived
dopaminergic neurons cultured on top of a collagen hydrogel,
expressing tyrosine hydroxylase (in magenta), GAP43 (in yellow)
and DNA (in cyan). Image credit: Ana Marote from ICVS, Universityof Minho and Leonor Ribeiro from INL.

Widefield

BC43 and Imaris Quant features for cancer studies:
3 Image a wide range of cells and tissues.
3 Powerful tools for fast, reproducible results.
3 AI-based tools for easy image analysis.
3 Generate spatial and morphological measurements.
3 Calculate statistics to compare different experimentalgroups.
3 With additional Imaris modules measure colocalizationand track cells.

A

B

C

Right: Confocal image of mammalian cells in division. A) Normal cell division. B andC) Abnormal cell division. Cancer cells most often have abnormal cell division. In manycases, these cells do not have a bipolar mitotic spindle (as seen in A) but have multiple
poles (as seen in B and C). These multiple poles can lead to abnormal separation of the
genomic content, and the daughter cells will have multiple copies of certain genes and no
copies of others. This is often named as “genome instability” which is a marker of cancer
cells. Image credit: Claudia Florindo, Andor Technology.

Widefield & ClearViewTM
Widefield image primary rat hippocampal neurons, after 15 daysin vitro, stained for Tubulin (yellow) and Nucleus (blue).
Image credits: Prof. Michael Kiebler; Sabine Thomas; Lehrstuhl fürZellbiologie, Biomedizinisches Centrum (BMC), Medizinische Fakultät,
LMU München

1011Introduction | Applications | Software | Features | Specifications | Ordering

BC43 for Core Facilities

Integrated Software Solutions

Small in size, Big in performance

FusionBC43 is an ideal instrument for a core facility, easy to operate, with multiple microscopy
techniques. It provides great images fast, whatever the sample. Free up your more complex
imaging systems for users doing highly specialised experiments.
Many imaging systems can be difficult for users to get comfortable using without extensive
training. BC43 is intuitive and easy for even novice microscopists to master. Simple operatingprocedures, and minimal maintenance allow exceptional productivity from the system. Thismeans less time training, more time imaging and more time for core staff running thefacility.
BC43 features for core facilities:
3 Low maintenance.
3 Fast to learn, easy to use, minimal support.
3 Application versatility.

BC43 has an integrated, easy-to-use, and accessible acquisition software interface thatdelivers high-end imaging. Users benefit from easy protocol set up for multidimensional
experiments, such as one-click multi–position-montage and multiwell integration with an
intuitive user interface and workflow for protocol set up.
Fusion for BC43 supports real-time GPU-accelerated deconvolution reducing blur andbringing clarity. And the easy-to-use super resolution can provide enhanced detail for
demanding experiments. Seamlessly integrated, the 3D stitching allows the montage andvisualisation of multiple tiles to give the full context of your sample.

Imaris® Quant
Each BC43 model includes theintroductory Imaris Quant package. Fusion
for BC43 saves files in the Imaris IMS
format permitting easy transfer of data
into Imaris for further visualisation andimage analysis. The Quant package adds
additional visualization and processing
features including high-resolution
snapshots, creation of multi-dimensional
animations, and advanced isosurface
rendering of objects within your images.
The Quant package provides the corequantitative features of Imaris, including
AI-based analysis. Whether you area cancer researcher or neuroscientist
there are tools for measuring wide ranging Anaphase in mammalian cells. Image shows an isosurface renderingof an anaphase cell. Image credits: Álvaro Tavares, Ines Baião-Santos,
properties of cells and proteins of interest
CBMR Universidade do Algarve and Claudia Florindo, Andor Technology.
in your samples. Additional applicationspecific modules of Imaris are available andinclude specific measurements suited for cell & developmental biologists, neuroscientists
and many more disciplines within the life sciences.
To find out more about Imaris please see: imaris.oxinst.com

Whole-body flatfish at climax of development. Fish was stained with acetylated tubulin (Yellow) and myosin heavy chain (blue). Confocal
image acquired with BC43 using multiple tile acquisition and montage. 30 tiles acquired to compose the image. Each tile had 175 slices,
over a Z range of 521 μm. Image credit: Marco Campinho, CBMR Universidade do Algarve and Claudia Florindo, Andor Technology.

1213Introduction | Applications | Software | Features | Specifications | Ordering

Key Features of BC43 Family
Hardware Feature

BenefitSoftware Feature

High-speed confocal•5
imaging

3 3D optical sectioning with high background rejection. Eliminates blur.
3 Allows deep and large tissue imaging at speed for higher productivity.
3 Image fast dynamic events in thicker samples.

Easy super resolution

Widefield imaging

3 Image thin specimens/structures that do not require optical sectioning.
3 Highest sensitivity mode for samples super-sensitive to light, or todetect the weakest fluorophores signals.

ClearViewTM GPU•5
accelerated deconvolution

3 Reduce blur and increase contrast with deconvolution.
3 Up to 50x faster processing than non-GPU based deconvolution
solutions.

Easy workflow

3 From sample insertion to image acquisition. Add sample, find sample,
set bounds, and acquire the image. No expertise required.
3 Quick montage - Faster acquisition and experimental setup, improve
productivity. Quick 3x3 sample overview, easy to set sample bounds,
and center sample for image acquisition with one-click.

•5

Benefit
3 For the highest resolution of subcellular structures in just a few
seconds.
3 Exceptional ease of use with Andor’s optimised SRRF-Stream+technology.

Differential phase contrast

3 Capture label-free images.
3 High contrast Andor transmitted light imaging modality.

Benchtop system

3 No need for a dark room. Fits in a small bench space in the laboratory.
3 Set up experiments and image immediately.

Patented Focus Seek & Lock

3 Focus Seek - makes focusing on your sample easier.
3 Focus Lock - maintains sample focus during long time-lapse andlarge sample acquisitions.

Built-in vibration
management mechanism

3 Ensures optimal image quality on your benchtop confocal whenworking at high-magnification and live-cell time-series.

Multidimensional acquisition

3 Acquire multiple imaging dimensions to visualise all the sample
features—simultaneous acquisition of time, Z and tile positions.

2x objective for quick sample
overview

3 Quickly navigate your sample with an overview montage and selectarea to image.

Multiposition

3 Acquire multiple positions in a sample and maximise throughput froma single experiment.
3 Multiposition montage - Acquire multiple montages at independent
positions and maximise throughput on fixed or live cell experiments.

3D ergonomic joystick

3 Efficient sample navigation, position and focus with adjustablenavigation and focus speeds.

Montage & Stitching

3 Automatically capture large sample data bigger than the field of view.
3 Stitch huge sample montages in 2D and 3D for the full picture.

Patented Borealis
illumination

3 Optimises illumination uniformity for seamless stitching and moreaccurate cross-field analysis.

Multiwell

3 Allow multiwell imaging for 6, 12, 24, and 96 well plates—image
different treatment, phenotypes, drug screening experiments, etc.

Total imaging flexibility

3 Image multiple fluorescent channels confocal and/or widefield.
3 Capture multiple imaging modalities in one protocol; fluorescencewith brightfield and Differential Phase Contrast.

Real-time 3D-rendering

3 Immediate visual feedback on experimental progress to evaluate data
and make appropriate decisions in real-time.

sCMOS detector

3 High sensitivity detector for short exposures and reduced
photobleaching.
3 Maximise number of cells in a single image and capture large
samples efficiently with a large field of view e.g. image a 1.85 mm
diagonal with 10x objective.
3 High dynamic range - capture weak and bright signals in a single
image without saturation.

Imaris Quant

3 Visualise their 2D/3D/4D images in the world’s leading interactive
microscopy image analysis software.
3 Identify and characterize objects within their images via traditional
intensity-based and AI methods.
Drosophila egg chamber stained with actin (yellow) and DNA (Cyan).
3 Create high-resolution
snapshots
withImage is a the stitchedresult andof 15multi-dimensionaltiles acquisition in with movies
2 confocal
ease.
channels, 176 Z steps over 70 um depth. Image credits, Rui Silva – CBMR

14

UAlg and Claudia Florindo.

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Introduction | Applications | Software | Features | Specifications | Ordering

Install and Quality Control Packages
Andor is proud to launch a new Installation Qualification (IQ) / Operational Qualification (OQ) programme
for its benchtop microscopes, offering reassurance on long-term performance and repeatability important todata presented in publications. Our OQ programme includes acceptance tests originating from engaging with
community groups such as QUAREP-LiMi. The aim, to meet expected standards for cross-industry comparisons
to be possible, if other manufacturers offer the same programmes. You can now have a fully supported quality
control program from the manufacturer of their microscope with detailed reports detailing actual performance
specifications.

Installation Qualification (IQ) Package
3

3
3

Field Service Engineer (FSE) installation - Our FSE will setup the BC43 system following our protocol and checksystem operation, once completed the engineer will deliver an installation certificate detailing the steps taken and thesuccessful installation as per our protocol.
Basic user training - Our FSE will train a small group of users on basic operations of the BC43 unit (approx. 2 hours).
Basic maintenance training - Training for two personnel, with certificates provided on completion.

Installation Qualification Operational Qualification (IQ/OQ) Package
3

3

The IQOQ package includes our IQ package plus our OQ quality control package, FSE will carry out a detailed seriesof tests to quantify system performance against our specification. Once performance has been successfully provenagainst our specification a performance certificate will be shared detailing exact performance characteristics of thesystem. If the performance variation is found to be due to environmental conditions our engineer will suggest steps toimprove performance.
The same series of tests will be carried out during the annual product maintenance included in your warranty and any
performance found to be outside of specification will be corrected - not including mechanical objective damage orchanges due to modifications in the environment.

Channel-pair Measurements
OQ TestBlue-to-Green Channel Pair
Specification

Green-to-Yellow Channel Pair
Specification

Green-to-Red Channel Pair
Specification

Full Field of View Maximum
Lateral Separation Distance(Lateral Co-registration)

Max |rxy| (Full FOV) ≤ 480 nm

Max |rxy| (Full FOV) ≤ 340 nm

Max |rxy| (Full FOV) ≤ 620 nm

Full Field of View Average Axial
Separation Distance (Axial Coregistration)

Avg |rz| (Full FOV) ≤ 505 nm

Avg |rz| (Full FOV) ≤ 410 nm

Avg |rz| (Full FOV) ≤ 870 nm

Full Field of View Channel
pair Co-registration (QUAREP
Normalization)

Max |rexp / rref| (Full FOV) ≤ 3.17

Max |rexp / rref| (Full FOV) ≤ 1.93

Max |rexp / rref| (Full FOV) ≤ 3.61

Central 30% Field of View
Maximum Lateral Separation
Distance (Lateral Coregistration)

Max |rxy| (30% FOV) ≤ 180 nm

Max |rxy| (30% FOV) ≤ 150 nm

Max |rxy| (30% FOV) ≤ 205 nm

Central 30% Field of View
Average Axial Separation
Distance (Axial Co-registration)

Avg |rz| (30% FOV) ≤ 505 nm

Avg |rz| (30% FOV) ≤ 410 nm

Avg |rz| (30% FOV) ≤ 870 nm

Central 30% Field of View
Channel pair Co-registration(QUAREP Normalization)

Max |rexp / rref| (30% FOV) ≤ 1.55

Max |rexp / rref| (30% FOV) ≤ 1.07

Max |rexp / rref| (30% FOV) ≤ 1.89

Single-channel Measurements
OQ TestDetector Intensity Response

Operational Qualification (OQ) Package

Z-stage 3D Reconstruction Accuracy

At any point in your warranty, or as a compliment to your post warranty service contract, an OQ quality control package
may be ordered. This quality control service provides two quality control visits where the engineer will carry out the same
set of detailed tests and provide a performance certificate at each visit detailing your exact system performance.

XY-Stage Positioning and Repeatability Precision

A full list of systems tests with descriptions is available on our website.

Central 30% Field of View Average Lateral Resolution (QUAREP Normalization)

Central 30% Field of View Average Lateral Resolution

Full Field of View Average Lateral Resolution

Channel Dependent Measurements
OQ TestLaser Power*
System Uniformity

Full Field of View Average Lateral Resolution (QUAREP Normalization)

Blue Channel
Specification 405 nm
Excitation 435-455 nm
Emission

Green Channel
Specification 488 nm
Excitation 517-541 nm
Emission)

Yellow Channel
Specification 561 nm
Excitation 580-610 nm
Emission

Red Channel
Specification 638 nm
Excitation 671-745 nm
EmissionP ≥ 12.5 mWP ≥ 8.5 mW

P ≥ 9.5 mWP ≥ 12.0 mWU ≥ 20%U ≥ 65%U ≥ 65%U ≥ 65%Central 30% Field of View Average Axial Resolution

C ≥ 65%Centering performance for other channels exceeds our measuring sensitivity*This test uses the epifluorescence (widefield) imaging mode of the microscope. All other tests utilize the confocal imaging mode of the
microscope.

16

R2Int ≥ 0.96
0.97 ≤ G ≤ 1.03
Max |Driftxy| ≤ 2.0 µm pk-pk
Avg FWHMxy (30% FOV) ≤ 280 nm
Avg FWHMxy / resox/y (30% FOV) ≤ 1.47
Avg FWHMxy (Full FOV) ≤ 280 nm
Avg FWHMxy / resox/y (Full FOV) ≤ 1.47
Avg FWHMz (30% FOV) ≤ 725 nm

Central 30% Field of View Average Axial Resolution (QUAREP Normalization)

Avg FWHMz / resoz (30% FOV) ≤ 1.34

Full Field of View Average Axial Resolution

Avg FWHMz / resoz (Full FOV) ≤ 1.34

System Vibration*
Contamination and Background Artifacts

Illumination Centering

Specification

σσxy ≤ 40 nm
Transmitted light Brightfield and FluorescenceBackground images must match factory reference
images (See test description for more details)*This test uses the epifluorescence (widefield) imaging mode of the microscope. All other tests utilise the confocal imaging mode of the
microscope.

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Introduction | Applications | Software | Features | Specifications | Ordering

Specifications

1

Microscope Unit BC43
Core Imaging Modes

Widefield epifluorescence
Transmitted light - brightfield and Differential Phase Contrast

High-speed confocal•5

Microlens spinning disk technology for instant confocal imaging.

ClearView™ GPU•5

Clears image of non-specific sample background signal and improves resolution beyond
the normal optical limits.

Super resolution•5

Increases image resolution to reveal structural detail down to 140-180 nm (depending on
sample type, preparation and resulting fluorescence signal to noise).

Imaging Methods

Single colour, multicolour, z-stacking (volume), time-lapse, multi-position, multi-well,montage and 2/3D stitching.

Mechanical Drawings
Units: Millimeters [Inches]

CameraResolution
QE•2

Field of view (mm)6.5 μm pixel; 2040 x 1992 (4.1 MP)
Up to 82%
18.5 mm (diagonal)

Cooling

0oC

Images16-bit, monochrome

Illumination
Fluorescence
Transmitted light

Fixed configuration of 405 nm, 488 nm, 561 nm, 640 nm
Broad spectrum visible light LED illumination with monochrome (greyscale) detection

Optics (Objectives)
Objective Lens Nosepiece

Motorised 5 position turret

Objective Magnifications

BC43 is supplied with 2x objective for sample overview.
Select additional supported objective lenses from 10x to 100x magnification.

Precision motorised x, y stage
Z-Control & Focus
Autofocus "Seek & Lock" Technology

Travel Range = 110 mm x 80 mm
Resolution = 100 nm
Range = 14.5 mm
Sample "Seek & Lock". Finds focal plane for new sample and maintains focus stability•3
during time-lapse experiments.

Sample Vessels Supported

Glass slides (25 by 75 mm); culture dish (35 mm diameter); Multiwell plates (6, 12, 24 &96); Multiwell chamber coverslip (2, 4, 8). Optimal imaging through glass, imaging throughplastic sample vessels are supported with extra-long working distance objectives.

Incubation (option)

Stage-top incubator. Sliding lid for easy sample access and exchange. Objective heater for
oil-immersion objectives.

Workstation

PC

Monitor18

Windows™ 11 operating system
64 GB DDR4 RAM
512 GB SSD Boot drive
16 GB Graphics Card
2 TB Image data storage (option to add more)
Fusion control and Imaris Quant Package software•4
27 inch19

Introduction | Applications | Software | Features | Specifications | Ordering

Creating the Optimum Product for you
Please contact your local sales representative who will be able to guide you through the ordering process.

ModelsStep 1.

Software & Workstation Options
Choose the model

Step 5.
Description

Order CodeAndor BC43 Benchtop Microscope.
• Brightfield and Differential Phase Contrast transmitted white light illumination forlabel-free visualisation and imaging, widefield epifluorescence for low-light imaging.

Description

Order CodeImaris Quant Package is installed on a separate offline PC for image analysis. NOT node
locked to the acquisition workstation.
Imaris Viewer will be installed with the BC43 workstation.

IMARIS-QUANT-SEP

ImarisImage Analysis Imaris Quant Package and add-on, if purchased, is installed on the BC43 workstation.
Node-locked to the acquisition workstation.
Software• Built-in vibration management to support use on a regular laboratory bench or sturdy
table.

Model

Choose Software Setup• Patented Focus Seek and Lock to aid sample focus and to retain focus during timelapse experiments.
• 2x objective included to aid sample navigation. 4 additional positions available on
the motorized turret to add objectives appropriate to your needs. Support up to 100x
(choose from list of supported objectives).
• Excitation lines 405 nm, 488 nm, 561 nm & 638 nm, and emission filter for imaging
commonly used fluorophores.

Step 6.

Workstation upgrade options (choose only 1)

INS-BM• Motorised x,y and z axis sample positioning via joystick or software interface.
• 4.1 MP (6.5 µm pixel, 16-bit) monochrome camera with up to 82% QE. 18.5 mm
diagonal field of view.

Options• Control software to capture multi-dimensional experiments in x,y,z, time, multiposition, multi-well and montage capture with 2/3D stitching.
• Imaris Quant Package for downstream image editing, multi-dimensional image
rendering, snapshots and animations, and AI-based object detection.•4
Andor BM42 Benchtop Microscope.
Same as INS-BM but cannot upgrade with confocal.

Step 7.
INS-BM-NU

Optional Imaging Modes or In-field Upgrade Paths

Confocal
Step 3.

Confocal upgrade (not for BM42)
Description

Order Code

Confocal module - microlens based spinning disk confocal for fast high-contrast highquality 3D imaging and deep imaging of thick samples.

INS-CF

Super resolution upgrade
Description

Super resolution
Step 4.

Deconvolution

20

Super resolution - Adds a easy-to-use super-resolution imaging mode which can achieve
resolution down to ~140 nm (depending on sample and strength of labelling). Good forlabelling structures like actin filaments, microtubules and vesicles.

Description

Order Code

4 TB data storage upgrade for supplied PC workstation

INS-PC-DRV-4TB

8 TB data storage upgrade for supplied PC workstation

INS-PC-DRV-8TB

Objectives• Workstation supplied see Specifications for full information.

Step 2.

IMARIS-QUANT-ACQ

Order Code
INS-SR

Deconvolution upgrade
Description

Order Code

ClearViewTM deconvolution - Improves image clarity (removes haze) and resolution. Can
be purchased with a widefield model. Please note ClearView is included with all confocalmodels as standard.

INS-DC

Objectives

Select up to 4 supported objective lenses
Description

NA

Working
Distance / mm

Compatibility

Order Code

10x Plan Fluorite objective

0.3

16

Widefield/Confocal

INS-OBJ-10-030

10x Plan Apochromat objective

0.45

4

Widefield/Confocal

INS-OBJ-10D-045

20x Plan Apochromat objective

0.8

0.8

Widefield/Confocal

INS-OBJ-20D-080

20x S Plan Fluorite objective

0.7

2.3

Widefield/Confocal

INS-OBJ-20-070LWD

40x Plan Fluorite objective

0.75

0.66

Widefield/Confocal

INS-OBJ-40-075

40x Plan Apochromat objective

0.95

0.21

Widefield/Confocal

INS-OBJ-40D-095

40x Plan Apochromat silicon oil
objective

1.25

0.3

Widefield/Confocal

INS-OBJ-40S-125SIL

40x Plan Fluorite oil immersion
objective

1.3

0.24

Widefield/Confocal

INS-OBJ-40-130-O

60x Plan Apochromat oil immersion
objective

1.42

0.15

Widefield/Confocal/
Super resolution

INS-OBJ-60D-142-O

100x Plan Apochromat oil
immersion objective

1.45

0.13

Widefield/Confocal/
Super resolution

INS-OBJ-100D145-O

20x Plan Fluorite air objective

0.5

2.1

Widefield

INS-OBJ-20-050

10x Plan Fluorite air objective

0.3

15.2

20x Plan Fluorite air objective

0.45

8.2-6.940x Plan Fluorite air objective

0.6

3.6-2.860x Plan Fluorite air objective

0.7

2.6-1.8

INS-OBJ-10-030-TC
Widefield (supports
plastic vessels)

INS-OBJ-20-045-TC
INS-OBJ-40-060-TC
INS-OBJ-60-070-TC

21

Introduction | Applications | Software | Features | Specifications | Ordering

Incubator Options
Step 8.

Incubators

Step 9a.

Incubator
SampleHolders

Step 9b.

Incubator
Accessories

22

Installation Options - Confocal Models Only
Step 10.

Select the required incubator
Description

Order CodeStage-top incubator with humidity module and digitally controlled CO2 regulation using
a pure CO2 source

INS-INC-HUM-CO2-D

Stage-top incubator with humidity module and manual valve-controlled CO2 regulation
using a pure CO2 source

INS-INC-HUM-CO2-M

Stage-top incubator with humidity module and manual valve-controlled CO2 regulation
using a pre-mix air/CO2 cylinder

INS-INC-HUM-PRE-M

Only available at time of original orderService

Select the required incubator sample holders
Description

Order CodeOne position. 1x3 inch chamber slide holder

MSD-INCB-1XGS-M

One position. 35 mm Petri-dish holder

MSD-INCB-1X35-M

Two position. 35 mm Petri-dish holder

MSD-INCB-2X35-M

One position. 1x3 inch chamber slide and #2 35 mm Petri-dish holder

MSD-INCB-GS35-M

Open frame for multi well plates, suitable for oil immersion objectives

MSD-INCB-MW-OIL

Two position. 1x3 inch chamber slide holder

MSD-INCB-2XGS-M

One position. Lab-Tek 1x2 inch chambered cover glass holder

MSD-INCB-1XLBTK-M

Two position. Lab-Tek 1x2 inch chambered cover glass holder

MSD-INCB-2XLBTK-IIM#1 Lab-Tek II 1x2 inch chambered cover glass and #1 50/60 mm Petri-dish holder

MSD-INCB-LBTK-II-60M#2 Lab-Tek 1x2 inch chambered cover glass holder

MSD-INCB-2XLBTK-M#1 Lab-Tek II 1x2 inch chambered cover glass holder

MSD-INCB-1XLBTK-IIM

Select the required incubator accessories
Description

Order CodeMagnetic holder for 35 mm petri dish.

MSD-INCB-35-TL-M

Lid with thermocouple for local / sample temperature recording at the level of the sample.

MSD-INCB-SENSOR

Select one of our Install or Operational Qualifications (optional)
Description

Order CodeInstallation Qualification (IQ): A field service engineer will install your new benchtop
microscope following a standardized protocol that confirms the instrument is working as
expected. An IQ certificate will be provided. Following the IQ install the service engineer
will provide basic user training for 2x users and will also provide extra slides and training toperform quality control tests, including laser power.

BC43-IQ

Installation Qualification (IQ): A field service engineer will install your new benchtop
microscope following a standardized protocol that confirms the instrument is working as
expected. An IQ certificate will be provided.
Operational Qualification (OQ): A field service engineer will perform a comprehensive
series of quantitative tests to detail the performance of your benchtop microscope and acertificate provided for reference.

BC43-IQOQ

Maintenance Options
Step 11.

Service

Select a maintenance plan (optional)
Description

Order Code

Platinum service plan for BC43 confocal models. Priority help desk, all parts, labour,
software updates. 1X annual preventative maintenance. Excludes consumables.
Replace XX with 12, 24 or 36.

BC43-PLAT-XXMTHSSilver service plan for BC43 confocal models. Priority help desk, labour, software
updates. 1X annual preventative maintenance. Excludes parts and consumables.
Replace XX with 12, 24 or 36.

BC43-SILV-XXMTHS

Platinum service plan for BM42 and BC43 WF widefield models. Priority help desk,
all parts, labour, software updates. 1X annual preventative maintenance. Excludes
consumables. Replace XX with 12, 24 or 36.

BC43WF-PLAT-XXMTHSSilver service plan for BM42 and BC43 WF widefield models. Priority help desk,
labour, software updates. 1X annual preventative maintenance. Excludes parts andconsumables. Replace XX with 12, 24 or 36.

BC43WF-SILV-XXMTHSOur OQ package is available for products out of warranty but still under a Silver or Platinum Maintenance contract.
Description

Order CodeOperational Qualification (OQ): A field service engineer will perform a comprehensive
series of quantitative tests to detail the performance of your benchtop microscope anda certificate provided for reference. This can be a requested service at any time forreassurance of ongoing performance of your benchtop microscope.

BC43-OQ

23

Order Today
Need more information? At Andor we are committed to finding the correct
solution for you. With a dedicated team of technical advisors, we are able tooffer you one-to-one guidance and technical support on all Andor products.
For a full listing of our local sales offices, please see:andor.oxinst.com/contact

Our regional headquarters are:Europe					Japan
Belfast, Northern Ireland 			Tokyo
Phone +44 (28) 9023 7126			
Phone +81 (3) 6744 4703
Fax +44 (28) 9031 0792			
Fax +81 (3) 3446 8320
North America				China
Concord, MA, USA 				Beijing | Shanghai | Guangzhou
Phone +1 (860) 290 9211			
Phone +86 (400) 678 0609
Fax +1 (860) 290 9566			
Fax +86 (10) 5884 7901

Items shipped with BC43

Footnotes: Specifications are subject to change without noticeBase unit, cables and accessories (model asordered)PC Workstation and accessories
Fusion and Imaris Quantify Package
User guides in electronic format
Quick start guide
Up to 5 microscope objectives
3D navigation joystick
Microscope slides

1. Figures are typical unless otherwise stated.

Operating & Storage Conditions:

7. Max power consumption during standard operation.

•
•
•
•
•
		

Indoor use only
Operating Temperature: 18°C to +25°C ambient
Storage Temperature: 0°C to 50°C
Relative Humidity: <70% (non-condensing)Size/Weight (BC43)
W x D x H: 505 x 633 x 447 mm and 67.5 kg

Power Requirements:• Mains Supply: 100 - 240 VAC, 50 - 60 Hz.

2. Quantum efficiency as supplied by the sensor manufacturer.
3. The Find Coverslip feature is not compatible with the 2x and oil immersion objective lenses. The
Focus Stabilization feature cannot be used with the 2x objective lens or plastic sample vessels.
4. Imaris Quantify Package software supplied, additional modules will require a separate license.
5. Please note confocal, ClearViewTM and super resolution functionality will depend on model or
upgrade selected.
6. Average power consumption when system is actively acquiring data.

Laser Safety Information
1. It is very hard to access the laser beam with the eye
without using a reflective surface to redirect it.
2. Class 2 means that the eye aversion response
protects against the laser radiation and you have todeliberately stare at it to cause damage. A typicalClass 2 product is a laser pointer.

Power Consumption / W
SystemcomponentStandbyor sleep

Typical

Unit

12

60 •6

95

PC

1.5

140 •6230 •7

Monitor

0.5

18

35

Max

Cover Image: Organoid imaged with BC43.
Pancreatic ductal adenocarcinoma cells insideand on top of a 3D hydrogel. DNA is cyan, lamin
A/C is Yellow and tubulin is magenta. Image
courtesy of Dr Sebastian Amos and Dr Yu-Suk
Choi, University of Western Australia.

BC43PESS 0824 R1