TuCam1024 x 1024 sensor, 26 fps
Large Field of View EMCCD
Ultimate Speed and Sensitivity

Two Camera
Imaging Adapter

Multi-Wavelength Imaging

Features and Benefits• Largest aperture		 Unique 22 mm aperture for large format		 sensors e.g. Neo and Zyla sCMOS• High quality achromatic lenses		 Image from 425 - 700 nm with minimal		 adjustment

Andor TuCam - High Performance, Two Camera
Imaging Adapter
Andor’s TuCam is a new generation two-camera adapter for macro or microscopic imaging
applications. Available in C- or CSU-X-mount, TuCam features include large aperture, exceptionaltransmission, very low distortion and high precision alignment using kinematic cassettes.
TuCam can be configured for simultaneous imaging from two similar cameras or as a switch• Highest transmission> 96% @ 425 - 700 nm

between camera models with different imaging capabilities.

• Very low distortion< 0.5%

precision alignment. These include wavelength and polarization splitters of the highest quality as• Bypass mode		 Dovetail mount for precise insertion,		 exchange and bypass of optical elements• Robust, compact and accurate		 Rigid structure provides optical and 				 mechanical stability• Convenient user adjustment		 User-controls for focus adjustment and		 2-axis cassette alignment are accessed via		 the front porch• C-mount and CSU versions		 Couple directly to filter wheels, 				 microscopes, C-lenses and spinning disk		 confocal scanners• Various magnifications		 Match cameras to CSU aperture or control		 effective pixel size

A full range of beam splitting optics are available with custom-designed kinematic cassettes forwell as a first surface mirror for switching between cameras.
A variety of camera tubes and lenses is available to provide magnifications of 1.0x, 1.2x, 1.5x and
2.0x indepently for each arm of the adapter. A filter wheel can also be integrated at the input ofTuCam to enable pre-filtering of the desired emission band.

Specifications Summary
Wavelength range

400 - 750 nm

Aperture size

22 mmTransmission> 96%

Application Guide Real time multi color imaging

 Biplane / dual focal plane imaging

 Co-localization

 Calcium flux / ion signalling e.g. Fura, Fluo-3 dyes

 Fluorescence Resonance Energy
Transfer (FRET) Dual wavelength TIRF microscopy Ratiometric imaging
 Super resolution
 Anisotropy imaging (inc. homo-FRET) Dual wavelength real-time confocal microscopy Fluorescence In Situ Hybridization (FISH) imaging
 Simultaneous fluorescence /DIC imaging

1

TuCam1024 x 1024 sensor, 26 fps
Large Field of View EMCCD
Ultimate Speed and Sensitivity

Two Camera
Imaging AdapterSpecifications•1

Wavelength range

400 - 750 nm

Throughput (C-mount version) •2> 96% (425-675 nm)

Throughput (CSU-X version) •2> 93% (425 to 675 nm)

Chromatic aberration (focus shift) •3< ± 0.2 mm (486 to 656 nm)

Distortion •4< 0.5%

Differential distortion •5< 0.5%

Maximum sensor format

22 mm diagonal

Field uniformity •6
Chromatic magnification variation •7Camera field alignment error •8> 90%< 25 µm (425 - 675 nm)< 32 µm

For detailed notes on performance figures annotated above, please refer to the last page of this specifications sheet.

Internal Optics
CSU-X version

C-mount version

2

TuCam1024 x 1024 sensor, 26 fps
Large Field of View EMCCD
Ultimate Speed and Sensitivity

Two Camera
Imaging Adapter

Dichroics and Polarizing Beams Splitters
Moxtek®

Semrock®
100%

90%90%80%80%

Reflectance (Rs)

Transmission (Tp)

70%
70%

Transmission

Transmission

60%60%

50%

FF484-FDi01

FF509-FDi01

FF580-FDi01

FF662-FDi01

50%

40%

40%

30%

30%

20%20%

Transmission (Ts)

10%

10%

Reflectance (Rp)

0%

0%
400

450500550600650

700450750500550600650

700

Wavelength (nm)Wavelength (nm)The graphs above show the transmission and reflectance curves for Semrock imaging dichroic beamsplitters and Moxtek optically flat polarizing
beamsplitters. Both types of beamsplitter are optimized for use at a 45° angle of incidence.
Semrock’s beamsplitters efficiently separate multicolored emission signals while maintaining excellent image fidelity. These dichroic beamsplitters areavailable for many popular fluorophore pairs. Their wide reflection and transmission bands and superb flatness allow for maximum light capture while
minimizing image aberrations.
The Moxtek beam splitters deliver good transmission and excellent contrast. Optically flat polarizing beamsplitters are a specific product engineered
for imaging applications. The quality of both the transmitted and reflected wavefront meet the exacting requirements of modern scientific instruments.

Transmission Curves
C-mount

CSU-X

99%

98%
97%

98%

96%
97%

95%

96%

93%

Transmission

Transmission

94%

92%
91%

95%

94%

90%
93%

89%
88%

92%

87%
91%

86%
400

450

500

550

600

Wavelength (nm)

650

700

750

400

450

500

550
600
Wavelength (nm)

650

700

750

Andor’s TuCam utilizes lenses with broadband anti-reflection coatings specifically chosen to maximise system throughput in the 400 to 750 nm
wavelength band. The CSU-X version of TuCam also includes broadband dielectric mirrors that are optimized for this region. The transmission curvefor the CSU-X version of the TuCam is shown on the left and for the C-mount version on the right. This is a typical performance for these instruments
and may vary slightly between individual units. Beam splitter optics are not included, please refer to the Semrock and Moxtek graphs above forperformance.

3

TuCam1024 x 1024 sensor, 26 fps
Large Field of View EMCCD
Ultimate Speed and Sensitivity

Two Camera
Imaging Adapter

Creating The Optimum Product for You
TR-DCI X - SUTexample shown

Step 1.

Choose the cooling optionChoose the mounting type
Simply select from the 2 mounting options that best suit your needs:Mounting

Step 2.

Description

CodeFor use with Revolution XD Yokogawa CSU-X fitted with customized external filter wheel

X-SUT

Any C-mount device including microscope or lens

S-CMT

Select the required magnification
TR-DCIX-SUT

Magnification

TR-DCIS-CMT (see note below)

Magnification required

Part CodeMagnification required

Part Code1x

TR-DCIX-100

1x

TR-DCIS-100

1.2x

TR-DCIX-120

1.2x

TR-DCIS-120

1.5x

TR-DCIX-150

1.5x

TR-DCIS-1502x

TR-DCIX-200

2x

TR-DCIS-200TR-DCIX-SUT: it is important to choose the correct magnification for each camera port (magnification can be the same ordifferent for each of the two ports). Refer to the ‘Camera Matching’ table on page 5.
TR-DCIS-CMT: 1x magnification is sufficient for all cameras. However, if a different effective pixel size is required, refer tothe ‘Camera Matching’ table on page 5 for the appropriate magnification.

Step 3.

Select the required Optical Cassettes
Please quote one or more of the following part numbers (we recommend one cassette per filter set to avoid risk of contamination
when changing operating wavelength):

OpticalCassettes

Step 4.

Description

Part CodeBlank cassette for mounting filter sets

TR-DCIS-CA1-00

Cassette with mirror - to allow switching between camera ports

TR-DCIS-CA4-01

Choose Wavelength or Polarization Filter Sets
Short Description

Wavelength& Polarization
Filter SetsLong Description

Part CodeGFP/RFP

Semrock FF01-514/30, FF02-617/73, Dichroic FF580-FDi01

TR-EMFS-F01

CFP/YFP

Semrock FF01-475/28, FF01-550/49, Dichroic FF509-FDi01

TR-EMFS-F02

Moxtek Wire Grid Polarizer BS PBF02C, 2 High Contrast cleanup filters
PPL04C

TR-EMFS-F03

CAMELEONS

Semrock FF01-483/32, FF01-542/27, Dichroic FF506-Di02

TR-EMFS-F05

GFP/YFP

Semrock FF01-497/16, FF01-550/32, Dichroic FF509-FDi01

TR-EMFS-F07

680/732 Filter Set

Semrock FF01-680/13, FF01-732/68, Dichroic FF700-Di01

TR-EMFS-F08

Polarizing filter set

Continued on page 5...

4TuCam
Step 4.

1024 x 1024 sensor, 26 fps
Large Field of View EMCCD
Ultimate Speed and Sensitivity

Two Camera
Imaging Adapter

Choose Wavelength or Polarization Filter Sets (continued from page 4)
Short Description

Step 5.

Part CodeSemrock FF01-579/34, FF01-679/41, Dichroic FF640-FDi01

TR-EMFS-F09

Cy3/Cy5.5

Semrock FF01-579/34, FF01-692/40, Dichroic FF640-FDi01

TR-EMFS-F12

Semrock FF01-530/43, Chroma HQ615LP, Dichroic FF580-FDi01

TR-EMFS-F13

Semrock FF01-525/45, Semrock FF01-680/42, Dichroic FF580-FDi01

TR-EMFS-F14

50/50 BS Mirror

Chroma 50/50 beamsplitter

TR-EMFS-F15

GFP/mCherry

Semrock FF02-525/40, FF01-640/40, Dichroic FF580-FDi01

TR-EMFS-F17

GFP/Cy5Semrock FF01-534/42, FF01-655/40, Dichroic FF580-FDi01

TR-EMFS-F20

GFP/mCherry:wideSemrock FF01-534/42, FF01-641/75, Dichroic FF580-FDi01

TR-EMFS-F21

Fluo4/Fura Red

Wavelength& Polarization
Filter SetsLong Description

Cy3-Cy5

GFP/Cy5

Select the required Accessories
The optical height of the system is 110 mm. We recommend that cameras and microscopes are raised using one of thefollowing accessories:
Description

Part Code

Mounting feet for iXon Ultra, iXon Life and Neo cameras

TR-IXON-MNT-110

Mounting feet for Zyla cameras

TR-ZYLA-MNT-110

CSUX 110 mm Opt Axis Mount Kit

CR-CSUX-MNT-110

Mounting feet for Olympus IX71/81

TR-OLIX-MNT-110

Mounting feet for Nikon TE-2000

TR-NKTE-MNT-110

Mounting feet for Nikon Eclipse Ti-E

TR-NKTI-MNT-110

Mounting feet for Zeiss Axiovert 200 and Zeiss Axio Observer

TR-ZSAV-MNT-110

Accessories

The following accessory is also available to enable Nomarski DIC imaging and user alignment of new dichroics with thecalibration slide:
TR-DCIS-DIC-MNTInternal mounting for DIC polarizer

Camera Matching
Magnification Required to Fill Sensor:
when fitted to CSUX orto alter effective pixel size (when attached to TR-DCIS-CMT)

Andor Camera Type

Sensor Format

Pixel Size

Zyla sCMOS

2560 x 2160

6.5 μm1x •9

Neo sCMOS

2560 x 2160

6.5 μm1x •9iXon Ultra/Life 897

512 x 512

16 µm

1.2x (13.3 μm effective pixel size)•10

iXon Ultra/Life 888

1024 x 1024

13 µm2x (6.5 μm effective pixel size)•10

Note: We minimize chromatic aberrations in our systems, but other optics have their own limitations. Chromatic errors in the microscope objective are small, but scaleby magnification at the detector. Consequently, we recommend software corrections for precision alignment.

5TuCam1024 x 1024 sensor, 26 fps
Large Field of View EMCCD
Ultimate Speed and Sensitivity

Two Camera
Imaging Adapter

Product Drawings
Dimensions in mm [inches]

CSU-X version

C-mount version

Weights:
Cassette = 0.35 Kg [12 oz]
Main unit = 5.5 Kg [12 lb 2 oz]

Weights:
Cassette = 0.35 Kg [12 oz]
Main unit = 4 Kg [8 lb 13 oz]

Recommended Microscopy Software For TuCam
The following software packages have been verified under simultaneous dual camera acquisition mode, as well as
offering functionality to merge and analyze data from each channel.
Please see ‘Application and Technical Notes’ section of the Multi-Wavelength Imaging brochure for further details.

6TuCam1024 x 1024 sensor, 26 fps
Large Field of View EMCCD
Ultimate Speed and Sensitivity

Two Camera
Imaging Adapter

Order Today
Need more information? At Andor we are committed to finding
the correct solution for you. With a dedicated team of technicaladvisors, we are able to offer you one-to-one guidance andtechnical support on all Andor products. For a full listing of ourregional sales offices, please see: andor.com/contact
Our regional headquarters are:Europe 				Japan
Belfast, Northern Ireland 			Tokyo
Phone +44 (28) 9023 7126			
Phone +81 (3) 6732 8968
Fax +44 (28) 9031 0792			
Fax +81 (3) 6732 8939
North America				ChinaConcord, MA, USA 				Beijing
Phone +1 (860) 290 9211			
Phone +86 (10) 8271 9066
Fax +1 (860) 290 9566			
Fax +86 (10) 8271 9055

Items shipped with your system:
1x Grid slide
1x Target for alignment
1x Appropriate allen key set for installation
1x Quick installation and alignment guide
1x Individual system performance booklet

Operating and Storage Conditions• Operating Temperature: 15°C to 30°C ambient

Footnotes: Specifications are subject to change without notice.
1.

Specifications are based on the 1.2x CSU version of TuCam using a Semrock imaging-flat
dichroic beamsplitter and 2 iXon3 CCD cameras (512 x 512 format with 16 µm pixel size, 8.2mm x 8.2 mm image area)
2. System throughput is obtained from the optical model of the system which considers the lenscoatings, dielectric mirror performance, Fresnel effects, vignetting and internal transmittance
3. Chromatic focal shift is the shift in the back focal length for the wavelength range 486 to 656
nm. Focal shifts for wavelengths above and below these values may vary.
4. Distortion manifests itself as different parts of the object being reproduced with different
magnifications in the image after passing through an optical system. Distortion is expressed asa percentage deviation of a point in the image from the same point in the object.
5. Differential distortion is the difference in the distortion level of identical points in the two imaging
paths. This measure is dependent on the quality and setup of the optical system.
6. Field uniformity is a measure of the flatness of the intensity distribution whilst under uniform
illumination.
7. Chromatic magnification (lateral color) is evident for off axis rays and is a consequence of thedifferent wavelengths of light being refracted at differing angles as they traverse an opticalsystem. Overlaying images taken at different wavelengths will highlight this effect as thedifferent colors appear to have different magnifications. Careful selection of lens materials
minimise this effect.
8. Alignment error is the maximum difference in the position of a particular point in one of theimaging paths to that in the other. The central quadrant of the image will have pixel alignment
but due to optical affects the alignment error will increase towards the field edge.
9. When using an sCMOS camera, a sub-array of pixels matching the aperture, will be read.
10. Additional magnification alters image brightness. Use only if improving spatial resolution or fieldof view is crucial.

Semrock is a registered trademark of Semrock, Inc.
Moxtek is a registered trademark of MOXTEK Inc.

• Relative Humidity: < 70% (non-condensing)• Storage Temperature: -25°C to 50°C

MTuCamSS 0617 R17