DATA SHEET
20221027

Anti-GABAA receptor-1
Product Information:
1) Catalog #:Item Name:
Size :
Species :Product Code :RRID :
2) Catalog #:Item Name:
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Species :Product Code :RRID :
3) Catalog #:Item Name:
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Species :Product Code :RRID :MSFR101520
GABAA receptor-a1 pAb (Rb) 20ug
20μg (affinity-purified with antigen polypeptide)
RabbitGABAARa1-Rb-Af660
AB_2571571
MSFR101530
GABAA receptor-a1 pAb (Rb) 50ug
50μg (affinity-purified with antigen polypeptide)
RabbitGABAARa1-Rb-Af660
AB_2571571
MSFR101540
GABAA receptor-a1 pAb (GP) 20ug
20μg (affinity-purified with antigen polypeptide)
Guinea pig
GABAARa1-GP-Af440
AB_2571572
MSFR101550
GABAA receptor-a1 pAb (GP) 50ug
50μg (affinity-purified with antigen polypeptide)
Guinea pig
GABAARa1-GP-Af440
AB_2571572

Formulation : Liquid ; 200 g/ml in PBS with 0.05% NaN3.
Storage : Store at cool temp. (2-10˚C)
The antibody can be stored at 2-10˚C.

The antibody can be also aliquoted and stored at -80 ˚Cfor long-term storage. Avoid repeated freeze-thawing. Non-hazardous. No MSDS required.
Species : rabbit / guinea pig, polyclonal
Antigen : mouse GABAAR1,369-386aa(NM_010250)

Specificity : mouse (others not tested)
Immunoblot with the antibodies detects a band at 43 kDa in mouse brain homogenates andmultiple bands in HEK cell lysates transfected with GABA AR1, but not GABAAR2.
Immunohistochemistry labels synaptic membrane at GABAergic synapses and also labels extrasynaptic neuronal surface.

ご注意：本商品には 0.1 % 未満のアジ化ナトリウムが入っています。
大量の水で洗い流してください。

Distributor : NITTOBO MEDICAL CO., LTD
TEL : +81-(0)3-4582-5451
email : nmd-tokuhan@nittobogrp.com

誤って目や口に入ったり、皮膚に付着した場合はManufacture : FRONTIER INSTITUTE CO., LTD

DATA SHEET
20221027

Applications : In general, affinity-purified antibody is used at around 1 microgram/ml for
immunoblot and immunohistochemistry. The most appropriate concentration should bedetermined by users, because it depends on contents in given cells, tissues and organs.
Research Use : For research use only, not for use in diagnostic procedures.
Remarks : Detection of synaptic GABAAR1 is enhanced by mild section pretreatment withpepsin, due to better antibody penetration into postsynaptic membrane.
Reference : 1) Ichikawa R, Yamasaki M, Miyazaki T, Konno K, Hashimoto K, Tatsumi H, InoueY, Kano M, Watanabe M: Developmental switching of perisomatic innervation from climbing
fibers to basket cell fibers in cerebellar Purkinje cells. J. Neurosci. 31:16916-16927, 2011.
2) Iwakura A, Uchigashima M, Miyazaki T, Yamasaki M, Watanabe M: Lack ofmolecular-anatomical evidence for GABAergic influence upon axon initial segment of cerebellar
Purkinje cells by the pinceau formation. J. Neurosci. 32:9438-9448.
3) Kudo T, Uchigashima M, Miyazaki T, Konno K, Yamasaki Y, Yanagawa Y, Minami M,
Watanabe M: Three types of neurochemical projection from the bed nucleus of the stria
terminalis to the ventral tegmental area in adult mice. J. Neurosci., in press

ご注意：本商品には 0.1 % 未満のアジ化ナトリウムが入っています。
大量の水で洗い流してください。

Distributor : NITTOBO MEDICAL CO., LTD
TEL : +81-(0)3-4582-5451
email : nmd-tokuhan@nittobogrp.com

誤って目や口に入ったり、皮膚に付着した場合はManufacture : FRONTIER INSTITUTE CO., LTD