රᗜ࣡࢖ࣝࢻࣛ࢖ࣇࣔࣀࢢࣛࣇ 6-8➨ 㸶 ❶            ࢽ࣍ࣥ࢖ࣀࢩࢩࡢ࢘࢖ࣝࢫឤᰁ⑕
⡿‶◊୕ 1࣭᭹㒊ᚿಖ 1࣭㕥ᮌ⤢Ꮚ 1࣭὾ᓮ༓⳯⨾ 1࣭ୗ⏣ ᐂ 1࣭๓⏣ ೺ 1
せ Ⅼ࢖ࣀࢩࢩ࡟ឤᰁࡍࡿ࢘࢖ࣝࢫឤᰁ⑕ࡢ୰࡛ࠊࣄࢺࡸ⏕⏘ື≀㸦≉࡟ࣈࢱ㸧࡟ᑐࡋ࡚ၥ㢟࡜࡞ࡿ E ᆺ⫢⅖࢘࢖ࣝࢫࠊ㔜⑕⇕ᛶ⾑ᑠᯈῶᑡ⑕ೃ⩌࢘࢖ࣝࢫࠊ࣮࢚࢜ࢫ࣮࢟
⑓࢘࢖ࣝࢫࠊ᪥ᮏ⬻⅖࢘࢖ࣝࢫࡢ␿Ꮫㄪᰝࢆᐇ᪋ࡋࠊࣄࢺࡸື≀࡬ࡢࣜࢫࢡ࡟ࡘ࠸࡚
⪃ᐹࡋࡓࠋ

Key words㸸࢖ࣀࢩࢩࠊE ᆺ⫢⅖࢘࢖ࣝࢫࠊ᪥ᮏ⬻⅖࢘࢖ࣝࢫࠊ㔜⑕⇕ᛶ⾑ᑠᯈῶᑡ⑕ೃ⩌࢘࢖ࣝࢫࠊ࣮࢚࢜ࢫ࣮࢟⑓࢘࢖ࣝࢫ

㸫ࡣࡌࡵ࡟
ࢽ࣍ࣥ࢖ࣀࢩࢩ㸦Sus scrofa leucomystax㸹௨ୗ࢖ࣀࢩࢩ࡜⾲グ㸧ࡣ㞧㣗ᛶ࡛࠶ࡾࠊ
㏆ᖺ㎰స≀➼ࢆồࡵ࡚ࣄࢺࡢ⏕άᅪ࡟౵ධࡍࡿࡼ࠺࡟࡞ࡗ࡚ࡁࡓࠋࡲࡓࠊྂ᮶ࡼࡾ࢖ࣀ
ࢩࢩࡢ⫗ࡣࠊ㣗⏝࡜ࡋ࡚ᾘ㈝ࡉࢀ࡚࠸ࡿࠋ࢖ࣀࢩࢩ࡟ឤᰁࡋࠊྠ᫬࡟ࣄࢺࡸࡑࡢ௚ࡢື
≀ࠊ≉࡟ࣈࢱ࡟ࡶឤᰁࡋ⑓Ẽࢆᘬࡁ㉳ࡇࡍ࢘࢖ࣝࢫ࡟ࡣࠊᅜෆ࡛ࡣ E ᆺ⫢⅖࢘࢖ࣝࢫࠊ
㔜⑕⇕ᛶ⾑ᑠᯈῶᑡ⑕ೃ⩌࢘࢖ࣝࢫࠊ࣮࢚࢜ࢫ࣮࢟⑓࢘࢖ࣝࢫࠊ᪥ᮏ⬻⅖࢘࢖ࣝࢫࠊ㇜࢖ࣥࣇ࢚ࣝࣥࢨ࢘࢖ࣝࢫࠊ㇜ࣃࣝ࣎࢘࢖ࣝࢫࠊ㇜⦾Ṫ࣭࿧྾ჾ㞀ᐖ⑕ೃ⩌࢘࢖ࣝࢫࠊ㇜
ࢧ࣮ࢥ࢘࢖ࣝࢫ 2 ࡞࡝ࡀ࠶ࡿ㸦⾲㸯㸧ࠋࡋ࠿ࡋࠊ࢖ࣀࢩࢩ࡟࠾ࡅࡿឤᰁ⑕◊✲ࡢṔྐࡣὸࡃࠊ◊✲ࡀ୙㊊ࡋ࡚࠸ࡿࡓࡵࠊ⾜ᨻࡸ⊁⊟⪅ࠊ୍⯡ᕷẸ࡞࡝⌧ሙࣞ࣋ࣝ࡟ࡲ࡛༑ศ࡞᝟ሗࡀఏࢃࡗ࡚࠸ࡿ࡜ࡣゝ࠸㞴࠸ࠋࡑࡇ࡛ᮏㄽ࡛ࡣࠊᡃࠎࡢ᭱᪂ࡢ◊✲ᡂᯝ㸦Hara et al.
In press; Takahashi et al. In press; ๓⏣ 2014; Mahmoud et al. 2011; Shimoda et al.
2013; Shimojima et al. 2011; Shimoda et al.; Ohno et al. 2009㸧࡟ࡼࡾุ᫂ࡋࡘࡘ࠶
ࡿࠊ࢖ࣀࢩࢩ࡟࠾ࡅࡿ E ᆺ⫢⅖࢘࢖ࣝࢫࠊ᪥ᮏ⬻⅖࢘࢖ࣝࢫࠊ㔜⑕⇕ᛶ⾑ᑠᯈῶᑡ⑕ೃ⩌࢘࢖ࣝࢫࠊ࠾ࡼࡧ࣮࢚࢜ࢫ࣮࢟⑓࢘࢖ࣝࢫ࡟㛵ࡍࡿឤᰁ≧ἣࡸឤᰁ⤒㊰ࡢ≉ᚩࢆ⾜ᨻᢸᙜ⪅ࡸ⊁⊟⪅࡞࡝ࡢᑐ⟇⪅ྥࡅ࡟⥲ㄽⓗ࡟⤂௓ࡋࠊࣄࢺࡸື≀࡬ࡢឤᰁࣜࢫࢡ࡟ࡘ࠸࡚⪃ᐹࡋࠊࡲࡓឤᰁ㜵ᚚ᪉ἲ࡟ࡘ࠸࡚ᥦゝࡍࡿࠋ࡞࠾ࠊᮏ◊✲࡛ࡣᢠཎ㸦࢘࢖ࣝࢫࡢ㑇ఏᏊ㸧᳨ฟ᳨ᰝ࡜ᢠయ᳨ฟ᳨ᰝࢆ⾜ࡗࡓࠋᢠཎ᳨ᰝࡣࢧࣥࣉࣜࣥࢢࡉࢀࡓᙜ᫬ࡢ࢘࢖ࣝࢫࡢឤᰁ᭷↓ࢆ᳨⣴࡛ࡁࡿ᳨ᰝ࡛࠶ࡿࠋ୍᪉ࠊᢠయ᳨ᰝࡣࠊ㐣ཤࡢឤᰁṔࢆ᳨⣴ࡍࡿࡇ࡜ࡀ࡛ࡁࡿ᳨ᰝ࡛࠶ࡿࠋᒣཱྀ኱Ꮫඹྠ⋇་Ꮫ㒊⑓ែไᚚᏛㅮᗙ⋇་ᚤ⏕≀Ꮫศ㔝 රᗜ࣡࢖ࣝࢻࣛ࢖ࣇࣔࣀࢢࣛࣇ 6-8⾲㸯㸬࢖ࣀࢩࢩ⏤᮶ࡢࣄࢺ࠾ࡼࡧື≀࡟ឤᰁࡍࡿྍ⬟ᛶࡢ࠶ࡿ୺せ࡞࢘࢖ࣝࢫឤᰁ⑕

( ᆺ⫢⅖( ᆺ⫢⅖ࡢ␿Ꮫ࡜୍⯡ᛶ≧E ᆺ⫢⅖ࡣࠊE ᆺ⫢⅖࢘࢖ࣝࢫ㸦௨ୗࠊHEV㸧ឤᰁ࡟ࡼࡗ࡚ᘬࡁ㉳ࡇࡉࢀࡿே⋇ඹ
㏻ឤᰁ⑕࡛࠶ࡿࠋⓎᒎ㏵ୖᅜ࡟࠾࠸࡚ࡣỈࢆ௓ࡋࡓ⣅ཱྀឤᰁ࡟ࡼࡾឤᰁࡍࡿ࡜࠸ࢃࢀ࡚࠾ࡾࠊ௨๓ࡣࢃࡀᅜ࡛ࡣὶ⾜ᆅ࡟Ώ⯟ࡋࡓࣄࢺࡀឤᰁࡍࡿࠊ㍺ධឤᰁ⑕࡜ࡋ࡚ㄆ㆑ࡉࢀ
࡚࠸ࡓࠋࡋ࠿ࡋࠊ2003 ᖺ 4 ᭶࡟රᗜ┴࡛ࢽ࣍ࣥࢪ࢝㸦Cervus Nippon㸹௨ୗࢩ࢝࡜⾲グ㸧ࡢ⫗ࡢ⏕㣗࡟ࡼࡿឤᰁ஦౛ࡀⓎ⏕ࡋࠊࡇࢀࡀ㣗ရ࡟ࡼࡿឤᰁࡢึࡵ࡚ࡢ☜ㄆ஦౛࡜࡞ࡗࡓ㸦Tei et al. 2003㸧ࠋࡑࢀ௨㝆ࠊE ᆺ⫢⅖ࡣ㣗ရ፹௓ᛶឤᰁ⑕࡜ࡋ࡚ㄆ㆑ࡉࢀࡿࡼ
࠺࡟࡞ࡗࡓࠋࡲࡓࠊᕷ㈍ࡉࢀ࡚࠸ࡿ㇜ࡢ⏕ࣞࣂ࣮࠿ࡽ HEV ࡀ᳨ฟࡉࢀࡓࡇ࡜ࢆࡣࡌࡵ
㸦Yazaki et al. 2003㸧ࠊ⌧ᅾࠊ㇜⫗ࠊ࢖ࣀࢩࢩ⫗ࠊࢩ࢝⫗ࡢ⏕⫗ࡢႚ㣗ࡀᅜෆ࡛ࡢࣄࢺ࡬ࡢឤᰁ※࡜ࡋ࡚㔜せどࡉࢀ࡚࠸ࡿࠋࡲࡓࠊE ᆺ⫢⅖࢘࢖ࣝࢫ࡟ࡣ 4 ✀㢮ࡢ㑇ఏᏊᆺࡀᏑᅾࡋࠊ1 ᆺࠊ2 ᆺࡀᾏእ࡛ὶ⾜ࡋ࡚࠸ࡿࡢ࡟ᑐࡋ࡚ࠊ3 ᆺࠊ4 ᆺࡣᅜෆ࡛ື≀ࡢ⫗࠿ࡽឤᰁࡋ࡚࠸ࡿࡇ࡜ࡶ▱ࡽࢀ࡚࠸ࡿࠋ⌧ᅾࠊឤᰁ⑕ࡢண㜵ཬࡧឤᰁ⑕ࡢᝈ⪅࡟ᑐࡍࡿ་⒪࡟㛵ࡍࡿἲᚊ㸦௨ୗࠊࠕឤᰁ⑕ἲࠖ
㸧࡟ᇶ࡙ࡁࠊE ᆺ⫢⅖ࡣឤᰁ⑕ἲࡢ➨ᅄ㢮ឤᰁ⑕࡟ᣦᐃࡉࢀ࡚࠾ࡾࠊᝈ⪅ࢆデ᩿ࡋࡓ་ᖌࡣಖ೺ᡤ࡟┤ࡕ࡟ᒆฟࡿࡇ࡜ࡀ⩏ົ࡙ࡅࡽࢀ࡚࠸ࡿࠋᮏ◊✲࡛ࡣࠊ᪥ᮏࡢ 3 ᆅᇦ࡛ᤕ⋓ࡉࢀࡓ࢖ࣀࢩࢩ࠾ࡼࡧࢩ࢝࠿ࡽᚓࡽࢀࡓ⾑ᾮࢆศᯒࡋࠊᢠయ࠾ࡼࡧᢠཎ㸦࢘࢖ࣝࢫࡢ㑇ఏᏊ㸧ࡢ᳨ฟࡢ୧ᐇ㦂࡟ࡼࡾࠊHEV ࡢឤᰁ≧ἣࢆศᯒࡋࡓࠋ රᗜ࣡࢖ࣝࢻࣛ࢖ࣇࣔࣀࢢࣛࣇ 6-8ᮦᩱ࠾ࡼࡧ᪉ἲ᳨ᰝᑐ㇟ᆅᇦ࠾ࡼࡧࢧࣥࣉࣝᩘ
୰ᅜ࣭஑ᕞ࣭㛵ᮾᆅ᪉࡟࡚ 2009 ᖺ࠿ࡽ 2013 ᖺ࡟ᤕ⋓ࡉࢀࡓ࢖ࣀࢩࢩࡼࡾᚓࡽࢀࡓ
⾑Ύࢆ⏝࠸ࡓࠋ࢖ࣀࢩࢩ⾑Ύࡢࢧࣥࣉࣝᩘࡣ୰ᅜᆅ᪉ 167ࠊ஑ᕞᆅ᪉ 46ࠊ㛵ᮾᆅ᪉ 152࡛࠶ࡗࡓࠋࡲࡓẚ㍑ᑐ㇟࡜ࡋ࡚ࠊ࢖ࣀࢩࢩ⾑Ύࢆ᥇ྲྀࡋࡓࡢ࡜ྠᆅᇦ࡛࠶ࡿࠊ୰ᅜᆅ᪉࡛ᤕ⋓ࡉࢀࡓࢩ࢝ࡼࡾᚓࡽࢀࡓ⾑Ύࡶศᯒ࡟⏝࠸ࡓࠋࢩ࢝⾑Ύࡢࢧࣥࣉࣝᩘࡣ 209 ࡛࠶ࡗࡓࠋ᳨ᰝ┠ⓗࡀ␗࡞ࡿࡓࡵࢧࣥࣉࣝᩘ࡟ࡣᕪࡀ࠶ࡿࠋ
+(9 ࡟ᑐࡍࡿᢠయࡢ᳨ฟELISA ἲ࡟ࡼࡾ⾑Ύ୰ࡢᢠయࢆ᳨ฟࡋࡓࠋELISA ᢠཎࡣࠊୗ㛵ࡢ HEV ᝈ⪅ࡢ㑇ఏᏊ㸦JTF-Yamagu11 ᰴ㸧ࢆᇶ࡟ N ᮎ➃㡿ᇦࢆḞᦆࡋࡓ ORF2 ࢆࢥ࣮ࢻࡍࡿ㑇ఏᏊࡣࣉࣛ࢖࣐࣮Yamagu11 ORF2 112F (ClaI)(5’-GTA TCG ATC ACC ATG GCT GTG GCTCCG GCC CCT-3’)࡜ Yamagu11 ORF2 660R-His (5’-GTA GAT CTT CAG TGA TGGTGA TGG TGA TGG TAC TCC CGG GTT TTA CCC A-3’)࡛ቑᖜࡋࡓࠋቑᖜࡋࡓ㑇ఏ
Ꮚࡣ ClaI ࡜ BglII ࡛ษ᩿ࡋࡓᚋࠊpCAGGS/MCS ࡢ ClaI ࡜ BglII ࢧ࢖ࢺ࡟ᤄධࡋࡓࠋᚓࡽࢀࡓⓎ⌧ࣉࣛࢫ࣑ࢻࢆ 293T ⣽⬊࡟࣏࢚ࣜࢳࣞࣥ࢖࣑ࣥࢆ⏝࠸࡚ࢺࣛࣥࢫࣇ࢙ࢡࢩࣙࣥࡋࡓࠋࢺࣛࣥࢫࣇ࢙ࢡࢩࣙࣥ⣽⬊ࡣ RIPA ࡟ࡼࡗ࡚ 4Υ1 ᫬㛫ฎ⌮ࡋࡓᚋࠊ15000
ᅇ㌿ 4Υ30 ศ㛫㐲ᚰࡋ࡚ୖΎࢆᅇ཰ࡋࡓࠋᢳฟᢠཎࢆ 5ȣg/mε࡟ᕼ㔘ࡋࡓᚋࠊ100ȣεࢆྛ࢙࢘ࣝ࡟᥋✀ࡋ࡚ ELISA ࢆ⾜ࡗࡓࠋࣈࣟࢵ࢟ࣥࢢᾮ࠾ࡼࡧᢠཎᕼ㔘ᾮ࡟ࡣࣈࣟࢵࢡ࢚࣮ࢫࢆ⏝࠸ࡓࠋ⾑Ύࡣ 1㸸100 ࡟ᕼ㔘ࡋࠊ஧ḟᢠయ࡟ࡣ࣌ࣟ࢟ࢩࢱ࣮ࢮᶆ㆑ProteinA/G ࢆ 1㸸10000 ᕼ㔘ࡋ࡚⏝࠸ࡓࠋⓎⰍ࡟ࡣ Bio-Rad ࡢࣃ࣮࢜࢟ࢩࢲ࣮ࢮᇶ㉁࢟ࢵࢺࢆ⏝࠸ࡓࠋ+(9 㑇ఏᏊ᳨ฟ⾑Ύ࠿ࡽ QIAamp Viral RNA Mini Kit ࢆ⏝࠸࡚ RNA ࢆᢳฟࡋࠊHEV-F1 ࣉࣛ࢖࣐࣮࡜ HEV-R2 ࣉࣛ࢖࣐࣮ࢆ⏝࠸࡚ RT-PCR ࢆᐇ᪋ࠊ᭦࡟ RT-PCR ⏘≀ࢆࠊHEV-F2 ࣉࣛ࢖࣐࣮࡜ HEV-R1 ࣉࣛ࢖࣐࣮ࢆ⏝࠸࡚ Nested PCR ࢆ⾜࠸ࠊ㑇ఏᏊࡢ᳨ฟࢆヨࡳࡓࠋHEV 㑇ఏᏊࡢ᳨ฟࢆヨࡳࡓࠋ㝧ᛶࡀ␲ࢃࢀࡿࢧࣥࣉࣝ࡟ᑐࡋ࡚ࡣࠊሷᇶ㓄ิࢆỴᐃࡋࠊ᭱⤊ⓗ࡟ุᐃࡍࡿ࡜࡜ࡶ࡟ࠊ㑇ఏᏊᆺࡢゎᯒࢆ⾜ࡗࡓࠋ⤖ᯝ࠾ࡼࡧ⪃ᐹ࢖ࣀࢩࢩ࡛ࡣ୰ᅜᆅ᪉࡟࠾࠸࡚ 75 㢌୰ 23 㢌㸦31%㸧ࠊ஑ᕞᆅ᪉࡟࠾࠸࡚ 46 㢌୰ 10㢌㸦22%㸧
ࠊ㛵ᮾᆅ᪉࡟࠾࠸࡚ 152 㢌୰ 12 㢌㸦8%㸧ࠊࢩ࡛࢝ࡣ୰ᅜᆅ᪉࡟࠾࠸࡚ 209
㢌୰ 1 㢌㸦0.5㸣㸧ࡀࠊHEV ࡟ᑐࡍࡿᢠయࢆಖ᭷ࡋ࡚࠸ࡓ㸦⾲㸰㸧ࠋᚑࡗ࡚ࠊHEV ࡟ᑐࡍࡿឤᰁ⋡ࡣᆅᇦᕪࡀ࠶ࡿࡶࡢࡢࠊ3 ᆅᇦ࡛ࡣࠊ࢖ࣀࢩࢩࢆឤᰁᐟ୺࡜ࡋ࡚ HEV ࡀศ රᗜ࣡࢖ࣝࢻࣛ࢖ࣇࣔࣀࢢࣛࣇ 6-8ᕸࡋ࡚࠸ࡿࡇ࡜ࡀ♧၀ࡉࢀࡓࠋࡲࡓࠊྠ୍ᆅᇦ࡛࠶ࡿ୰ᅜᆅ᪉࡟࠾࠸࡚ࡣࠊ࢖ࣀࢩࢩ࡜
ࢩ࢝ࡢ HEV ឤᰁ⋡ࡀ኱ࡁࡃ␗࡞ࡾࠊࢩ࢝ࡢឤᰁ⋡ࡣ㠀ᖖ࡟ప࠿ࡗࡓࡇ࡜࠿ࡽࠊࢩ࢝࡟
ࡘ࠸࡚ࡣࡇࢀࡲ࡛ࡢሗ࿌࡜ྠᵝ࡟ࠊHEV ࡢឤᰁࡣ㠀ᖖ࡟ࡲࢀ࡛࠶ࡿࡇ࡜ࡀ♧၀ࡉࢀࡓࠋ
HEV 㑇ఏᏊ᳨ᰝ࡟ࡼࡾࠊ࢘࢖ࣝࢫࡢ㑇ఏᏊࡢಖ᭷ࡢ᭷↓ࢆㄪ࡭ࡓࠋ࢖ࣀࢩࢩ࡛ࡣ୰
ᅜᆅ᪉ࡢಶయ 167 㢌୰ 6 㢌㸦4%㸧࠿ࡽࠊࢩ࢝࡟࠾࠸࡚ࡣ୰ᅜᆅ᪉ࡢಶయ 201 㢌୰ 1 㢌㸦0.5%㸧ࡢ⾑Ύ࠿ࡽ HEV 㑇ఏᏊࡀ᳨ฟࡉࢀࡓࡀࠊ஑ᕞ࠾ࡼࡧ㛵ᮾᆅ᪉ࡢ࢖ࣀࢩࢩ࠿ࡽ
ࡣ HEV 㑇ఏᏊࡣ᳨ฟࡉࢀ࡞࠿ࡗࡓ㸦⾲㸱㸧ࠋHEV 㑇ఏᏊࡀ☜ㄆࡉࢀࡓ୰ᅜᆅ᪉ࡢ࢖ࣀ
ࢩࢩ 6 㢌࡜ࢩ࢝ 1 㢌࠿ࡽ᳨ฟࡉࢀࡓ HEV 㑇ఏᏊᆺࢆゎᯒࡋࡓ⤖ᯝࠊࡇࢀࡽࡢ㑇ఏᏊࡣ㠀ᖖ࡟㏆⦕࡛࠶ࡗࡓࠋ᭦࡟ࠊྠᆅᇦ࡛Ⓨ⑓ࡋࡓࣄࢺࡢ E ᆺ⫢⅖ᝈ⪅࠿ࡽ᳨ฟࡉࢀࡓ㑇ఏᏊࡀࡇࡢࢡࣛࢫࢱ࣮࡟ྵࡲࢀࡓࠋࡲࡓࠊࡇࡢᆅᇦࡢ HEV ࡣࠊ᪥ᮏࢆྵࡴ௚ࡢᆅ᪉࠿
ࡽ᳨ฟࡉࢀࡓ HEV ࡜ࡣ␗࡞ࡿ⊂❧ࡋࡓࢡࣛࢫࢱ࣮ࢆᙧᡂࡋࡓ㸦ᅗ㸯㸧ࠋ௨ୖࡼࡾࠊ୰ᅜᆅ᪉࡛ࡣࠊ࠶ࡿ HEV ࡢ㑇ఏᏊᆺ࡟ࡼࡿ࢖ࣀࢩࢩࠊࢩ࢝ࠊࣄࢺ㛫࡛ឤᰁ⎔ࡀᡂ❧ࡋ࡚࠸ࡓࡇ࡜ࡀ♧၀ࡉࢀࡓࠋ⾲ 㸬+(9 ࡟ᑐࡍࡿᢠయಖ᭷⋡࢖ࣀࢩࢩࢩ࢝୰ᅜᆅ᪉ ஑ᕞᆅ᪉ 㛵ᮾᆅ᪉ ୰ᅜᆅ᪉ㄪᰝᩘᢠయ㝧ᛶᩘᢠయ㝧ᛶ⋡ 㸣 

⾲ 㸬⾑Ύ୰࡟࠾ࡅࡿ +(9 㑇ఏᏊ᳨ฟ⋡࢖ࣀࢩࢩࢩ࢝

୰ᅜᆅ᪉ ஑ᕞᆅ᪉ 㛵ᮾᆅ᪉ ୰ᅜᆅ᪉ㄪᰝᩘ㑇ఏᏊ㝧ᛶᩘ

㑇ఏᏊ㝧ᛶ⋡㸦㸣㸧






ᮏ◊✲ࢆྵࡵࠊ⾑ᾮ࠿ࡽ E ᆺ⫢⅖࢘࢖ࣝࢫ㸦HEV㸧ࡢ㑇ఏᏊࡀ᳨ฟࡉࢀ࡚࠸ࡿࠋࡍ࡞ࢃࡕࠊ࢖ࣀࢩࢩ࠾ࡼࡧࢩ࢝࡟࠾࠸࡚ࡣ඲㌟ࡢ➽⫗ෆ࠾ࡼࡧྛ⮚ჾ࡟ HEV ࡀᏑᅾࡍࡿࡇ࡜ࢆ♧ࡋ࡚࠾ࡾࠊ࢖ࣀࢩࢩ࠾ࡼࡧࢩ࢝ࢆႚ㣗ࡍࡿሙྜ࡟ࡣࠊ࡝ࡢ㒊఩࡟㛵ࡋ࡚ࡶ༑ศ࡟ຍ⇕ࢆࡍࡿᚲせᛶࡀ࠶ࡿࠋࡲࡓㄪ⌮࡛౑⏝ࡋࡓჾලࡶ༑ศ࡟Ὑίࡍࡿᚲせࡀ࠶ࡿ㸦ᅗ㸰㸧 රᗜ࣡࢖ࣝࢻࣛ࢖ࣇࣔࣀࢢࣛࣇ 6-8ᅗ  ࢖ࣀࢩࢩ࣭ࢩ࣭࢝ࣄࢺ࠿ࡽ᳨ฟࡉࢀࡓ +(9 㑇ఏᏊࡢ⣔⤫ゎᯒ࢖ࣀࢩࢩ⏤᮶ᰴࡣ㉥Ꮠ࣭⥳Ꮠࠊࢩ࢝⏤᮶ᰴࡣ㟷Ꮠࠊࣄࢺ⏤᮶ᰴࡣ⣸࡛♧ࡍࠋᅗ  ( ᆺ⫢⅖࢘࢖ࣝࢫࡢឤᰁࡢྍ⬟ᛶ㔜⑕⇕ᛶ⾑ᑠᯈῶᑡ⑕ೃ⩌ 6)76 ࢘࢖ࣝࢫ6)76 ࡢ␿Ꮫ࡜୍⯡ᛶ≧㔜⑕⇕ᛶ⾑ᑠᯈῶᑡ⑕ೃ⩌㸦SFTS㸧ࡣ SFTS ࢘࢖ࣝࢫ㸦௨ୗࠊSFTSV㸧ࡢឤᰁ࡟ࡼࡾ㸪ࣄࢺ࡟Ⓨ⇕ࠊⓑ⾑⌫ῶᑡࠊ⾑ᑠᯈῶᑡࠊ⫢㓝⣲ࡢୖ᪼࡞࡝ࢆᘬࡁ㉳ࡇࡍࠊ⮴Ṛᛶࡢ㧗࠸ே⋇ඹ㏻ឤᰁ⑕࡛࠶ࡿࠋ2011 ᖺ࡟୰ᅜ࡟࠾࠸࡚ึࡵ࡚ሗ࿌ࡉࢀࡓ⑓Ẽ࡛࠶ࡾࠊ୰ රᗜ࣡࢖ࣝࢻࣛ࢖ࣇࣔࣀࢢࣛࣇ 6-8ᅜ࡛ࡣࢲࢽࡀᮏ࢘࢖ࣝࢫࢆ፹௓ࡋࠊࡵࢇ⨺ࠊࣖࢠࠊ࢘ࢩࠊ࢖ࢾࠊࣈࢱࠊࢽ࣡ࢺࣜ࡞࡝ከ
ࡃࡢື≀ࡀ SFTSV ࡟ᑐࡍࡿᢠయࢆಖ᭷ࡋ࡚࠸ࡿ࡜ሗ࿌ࡉࢀ࡚࠸ࡿ㸦Yu et al. 2011ࠊᅗ
㸱㸧ࠋᅜෆ࡛ࡣ 2013 ᖺ 1 ᭶࡟ᒣཱྀ┴࡛ᅜෆึࡢ SFTS ᝈ⪅ࡀሗ࿌ࡉࢀࠊࡑࡢᚋࡢㄪᰝ
࡟ࡼࡾࠊ2005 ᖺ࠿ࡽ SFTS ᝈ⪅ࡀⓎ⏕ࡋ࡚࠸ࡓࡇ࡜ࡀุ᫂ࡋ࡚࠸ࡿ㸦Takahashi et al.
In press㸧ࠋࡑࡢ௚ SFTS ᝈ⪅ࡢⓎ⏕᫬ᮇࡣ 4 ᭶࠿ࡽ 10 ᭶࡛ࢲࢽࡢάື᫬ᮇ࡟୍⮴ࡍࡿࡇ࡜ࠊᖺ㱋ู࡛ࡣ 50 ṓ௨ୖࠊᆅᇦู࡛ࡣ୰ᅜࠊᅄᅜࠊ஑ᕞᆅ᪉࡟Ⓨ⏕ࡀከ࠸ࡇ࡜ࡀࠊ
≉ᚩ࡜ࡋ࡚ሗ࿌ࡉࢀ࡚࠸ࡿ㸦Takahashi et al. In press㸧ࠋ⌧ᅾࠊឤᰁ⑕ἲ࡟ᇶ࡙ࡁࠊᝈ⪅ࢆデ᩿ࡋࡓ་ᖌࡣಖ೺ᡤ࡟┤ࡕ࡟ᒆฟࡿࡇ࡜ࡀ⩏ົ࡙ࡅࡽࢀ࡚࠸ࡿࡀࠊᅜෆ࡟࠾ࡅࡿ SFTS ࡟㛵ࡍࡿ᝟ሗࡣᑡ࡞࠸ࠋᮏ◊✲࡛ࡣࠊ୰ᅜᆅ᪉ࡢ࢖ࣀࢩࢩ࠾ࡼࡧࢩ࢝࡟࠾ࡅࡿSFTSV ࡢឤᰁ≧ἣࢆศᯒࡋࡓࠋ༸⏘༸Ꮴ໬㔝⏕ື≀䞉䝠䝖䞉ᐙ␆ᡂ䝎䝙㻿㻲㼀㻿㼂㻿㻲㼀㻿㼂⬺⓶㻿㻲㼀㻿㼂྾⾑྾⾑྾⾑ᗂ䝎䝙⬺⓶ⱝ䝎䝙ᅗ  6)76 ࢘࢖ࣝࢫࡢឤᰁ⎔࡜㔝⏕ື≀࡜ࡢ㛵ಀᮦᩱ࠾ࡼࡧ᪉ἲ᳨ᰝᑐ㇟ᆅᇦ࠾ࡼࡧࢧࣥࣉࣝᩘ
୰ᅜᆅ᪉࡛ 2009 ᖺ࠿ࡽ 2013 ᖺ࡟ᤕ⋓ࡉࢀࡓ࢖ࣀࢩࢩ 115 㢌࠾ࡼࡧࢩ࢝ 130 㢌ࡢ⾑Ύࢆ⏝࠸ࡓࠋ රᗜ࣡࢖ࣝࢻࣛ࢖ࣇࣔࣀࢢࣛࣇ 6-86)769 ࡟ᑐࡍࡿᢠయࡢ᳨ฟ
SFTSV㸦HB29 ᰴ㸧ឤᰁ⣽⬊࠾ࡼࡧ mock ឤᰁ⣽⬊ࢆᢠཎ࡜ࡋࡓ ELISA ἲࢆ 100ࠊ
400ࠊ1600ࠊ6400 ಸᕼ㔘ࡋࡓ࢖ࢾ⾑Ύࢆ⏝࠸࡚ᐇ᪋ࡋࡓࠋ஧ḟᢠయ࡟ࡣ HRP ᶆ㆑
Protein A/G ࢆ⏝࠸ࡓࠋ⤖ᯝ࠾ࡼࡧ⪃ᐹ ୰ᅜᆅ᪉࡛ᤕ⋓ࡉࢀࡓ࢖ࣀࢩࢩ 115 㢌୰ 9 㢌㸦8%㸧
ࠊࢩ࢝ 130 㢌୰ 65 㢌㸦50%㸧ࡀSFTSV ࡟ᑐࡍࡿᢠయࢆಖ᭷ࡋ࡚࠸ࡓ㸦⾲㸲㸧ࠋࡲࡓࠊ᳃ᕝ࡯࠿㸦2013㸧ࡢ⤖ᯝ࠿ࡽࠊ㣫⫱≟ࡼࡾࡶ⊁⊟≟ࡢ᪉ࡀ SFTS ࢘࢖ࣝࢫᢠయࡢ㝧ᛶ⋡ࡀ㧗࠸ࡇ࡜ࡀሗ࿌ࡉࢀ࡚࠾ࡾࠊᒣᯘ࡟ධࡿᶵ఍ࡀከ࠸࡜ឤᰁ⋡ࡀ㧗ࡲࡿྍ⬟ᛶࡀ♧၀ࡉࢀࡓࠋ௨ୖࡼࡾࠊᅜෆึࡢ
SFTS ᝈ⪅ࡀ☜ㄆࡉࢀࡓᆅᇦ࿘㎶࡟⏕ᜥࡍࡿ࢖ࣀࢩࢩ࠾ࡼࡧࢩ࢝࡟࠾࠸࡚ࠊSFTSV ឤᰁṔࡀ࠶ࡿࡇ࡜ࠊ᪥ᮏ࡟࠾࠸࡚ࡶ㔝⏕ື≀࡟ SFTSV ࡢឤᰁࡀᡂ❧ࡍࡿࡇ࡜ࡀ♧ࡉࢀࡓࠋࡇࢀࡲ࡛ࡢሗ࿌࠿ࡽ SFTSV ࡣࢲࢽ࡟ࡼࡾ፹௓ࡉࢀࡿࡓࡵࠊᮏ◊✲⤖ᯝࡣࠊࡇࡢᆅᇦ࡟
⏕ᜥࡍࡿࢲࢽࡣ SFTS ࢘࢖ࣝࢫࢆಖ᭷ࡍࡿࡋ࡚࠸ࡿྍ⬟ᛶࡀ࠶ࡿࡇ࡜ࢆ♧၀ࡋ࡚࠸ࡿࠋࡇࡢྍ⬟ᛶࢆ⌮ゎࡋࠊࢲࢽ࡟ࡼࡿ่ညࢆ㜵ᚚࡍࡿᡭẁࢆྲྀࡿ࡜ඹ࡟ࠊSFTS Ⓨ⑕᫬ࡢ⑕≧ࡢ≉ᚩࢆࡼࡃ⌮ゎࡋࠊSFTSV ឤᰁࡀ␲ࢃࢀࡿሙྜ࡟ࡣ⮳ᛴ⑓㝔࡟⾜ࡁࠊࢲࢽ࡟่ညࡉࢀࡓࡇ࡜ࢆ࿌ࡆ࡚㎿㏿࡟デ᩿ࡋ࡚ࡶࡽ࠺ᚲせࡀ࠶ࡿࠋຍ࠼࡚ࠊࢲࢽ࡟ࡼࡾ፹௓ࡉࢀࡿ
ឤᰁ⑕ࡣࠊSFTS ௨እ࡟ࡶ᪥ᮏ⣚ᩬ⇕ࡸࣛ࢖࣒⑓ࠊࡘࡘࡀࡴࡋ⑓࡞࡝ᵝࠎ࠶ࡿࡓࡵࠊࢲࢽ࡟ࡼࡿ่ည࡟ࡣᬑẁ࠿ࡽ༑ศ࡟ὀពࡍࡿᚲせࡀ࠶ࡿࠋ⾲ 㸬6)769 ࡟ᑐࡍࡿᢠయಖ᭷⋡࢖ࣀࢩࢩࢩ࢝ㄪᰝᩘᢠయ㝧ᛶᩘᢠయ㝧ᛶ⋡  ᮏ◊✲࡛࢖ࣀࢩࢩࡢᢠయ㝧ᛶ⋡ࡀࢩ࢝࡟ẚ࡭࡚ప࠿ࡗࡓ⤖ᯝ࡟㛵ࡋ࡚ࡣࠊཎᅉࡣ⌧ᅾ᫂☜࡟ࡣ࡛ࡁ࡞࠸ࡀࠊSFTSV ࢆ፹௓ࡍࡿࢲࢽࡢ྾⾑ື≀࡟ᑐࡍࡿႴዲᛶࡀ㛵୚ࡋ࡚࠸ࡿྍ⬟ᛶࡀ⪃࠼ࡽࢀࡿࡓࡵࠊ௒ᚋࡢ◊✲ࡀᮇᚅࡉࢀࡿࠋ࣮࢚࢜ࢫ࣮࢟⑓࢘࢖ࣝࢫ࣮࢚࢜ࢫ࣮࢟⑓ࡢ␿Ꮫ࡜୍⯡ᛶ≧࣮࢚࢜ࢫ࣮࢟⑓ࡣ㇜࣊ࣝ࣌ࢫ࢘࢖ࣝࢫ 1㸦௨ୗࠊPRV㸧࡟ࡼࡗ࡚Ⓨ⑕ࡍࡿࠊࣈࢱ࠾ࡼࡧ࢖ࣀࢩࢩࡀᑐ㇟࡜࡞ࡿᒆฟఏᰁ⑓࡛࠶ࡾࠊᗂ㇜ࡀⓎ⑕ࡍࡿ࡜⮴Ṛ⋡ࡀ㧗࠸ࡓࡵࠊ㣴㇜ රᗜ࣡࢖ࣝࢻࣛ࢖ࣇࣔࣀࢢࣛࣇ 6-8ᴗ࡟ከ኱࡞⤒῭ⓗ⿕ᐖࢆཬࡰࡍࠋឤᰁࡣࠊ㰯Ồࡢ㣕ἓࠊᦤ㣗ࠊയཱྀ࠿ࡽࡢឤᰁࡸởᰁ≀ࡢᦤྲྀࡀཎᅉ࡜࡞ࡾࠊᡂ㇜࡛ࡣṚஸ⋡ࡣ㧗ࡃ࡞ࡃࠊከࡃࡢሙྜࡣ୙㢧ᛶឤᰁ㸨࡟⤊ࢃࡿࡇ࡜ࡀከ࠸ࠋࡋ࠿ࡋࠊࣈࢱࡸ࢖ࣀࢩࢩ௨እࡢື≀࡟ឤᰁࡋࡓሙྜࡣࠊ⚄⤒⑕≧࡛࠶ࡿᥙ⑛⑕ࢆᘬࡁ㉳ࡇࡋࠊ࡯ࡰ 100%Ṛஸࡍࡿࠋឤᰁᚋ࡟ PRV ࡟⪏㐣ࡋࡓࣈࢱ࡛ࡣࠊ࢘࢖ࣝࢫࡀ₯అឤᰁ㸨㸨ࡍࡿ≉ᚩࡀ࠶ࡾࠊᅜෆ࡛ࡣࠊࠕ࣮࢚࢜ࢫ࣮࢟⑓㜵␿ᑐ⟇せ㡿ࠖ࡟ᇶ࡙ࡁࠊ
ࣈࢱ࡟࠾࠸࡚ࡣ࣡ࢡࢳࣥࢥࣥࢺ࣮ࣟࣝ࡟ࡼࡾ 36 ┴࡛ PRV ࡢΎί໬࡟ᡂຌࡋ࡚࠾ࡾࠊ
11 ┴࡛Ὑί໬ᑐ⟇ࡀᐇ᪋ࡉࢀ࡚࠸ࡿ㸦㎰ᯘỈ⏘┬ᾘ㈝࣭Ᏻ඲ᒁື≀⾨⏕ㄢ㸧ࠋࡋ࠿ࡋࠊ⮫ᗋ⑕≧ࢆ♧ࡋ࡚࠸ࡿࣈࢱࡢ᦬Ⓨ࣭Ὲử௨እ࡟ࡶࠊ₯అឤᰁࡋ࡚࠸ࡿ㔝እ࢘࢖ࣝࢫᢠయ㝧ᛶࣈࢱࢆ᪩ᮇ࡟Ⓨぢࡍࡿᚲせࡀ࠶ࡿࠋ㏆␥ᆅ᪉ࡣࠊ᏶඲࡟࣮࢚࢜ࢫ࣮࢟⑓ࡢΎί໬࡟ᡂຌࡋ࡚࠸ࡓࡀࠊዉⰋ┴࡟࠾࠸࡚ࠊ1997 ᖺ࡟࢖ࣀࢩࢩࡢ⏕⫗ࢆ㣗࡭ࡓ⊟≟ࡀ࣮࢚࢜ࢫ࣮࢟⑓࡟ࡼࡾከᩘṚஸࡋࡓࡇ࡜ࡀሗ࿌ࡉࢀ࡚࠸ࡿ㸦ᖾ⏣࡯࠿ 2000㸧
ࠋᚑࡗ࡚ࠊ࢖ࣀࢩࢩ࡟ࡣ₯ᅾⓗ࡟࣮࢚࢜ࢫ࣮࢟⑓࢘࢖ࣝࢫࡀឤᰁࡋ࡚࠸ࡿྍ⬟ᛶࡀ㧗࠸࡜⪃࠼ࡽࢀࡿࡀࠊᅜෆࡢ࢖ࣀࢩࢩ࡟࠾ࡅࡿ࣮࢚࢜ࢫ࣮࢟⑓࢘࢖ࣝࢫࡢឤᰁ≧ἣ࡟ࡘ࠸࡚ࡣሗ࿌ࡀ࡞࠸ࠋᮏ◊✲࡛ࡣࠊ࣮࢚࢜ࢫ࣮࢟⑓ࡢ⾑Ύ␿Ꮫㄪᰝࢆᐇ᪋ࡋࡓࠋ㸨୙㢧ᛶឤᰁ㸸ឤᰁࡣࡋ࡚࠸ࡿࡀࠊⓎ⑓ࢆకࢃ࡞࠸ឤᰁࠋ㸨㸨₯అឤᰁ㸸࢘࢖ࣝࢫࡣ᳨ฟࡉࢀ࡟ࡃ࠸ࡀࠊᣢ⥆ⓗ࡟ឤᰁࡀᡂ❧ࡋ࡚࠸ࡿ≧ἣࠋᮦᩱ࠾ࡼࡧ᪉ἲ᳨ᰝᑐ㇟ᆅᇦ࠾ࡼࡧࢧࣥࣉࣝᩘࣈࢱ࡛ࡢ࣮࢚࢜ࢫ࣮࢟⑓ࡢΎί໬࡟ᡂຌࡋ࡚࠸ࡿ 3 ┴ࠊ㏆␥ᆅ᪉ࡢ A ┴࠾ࡼࡧ B ┴ࠊ୰ᅜᆅ᪉ࡢ C ┴࡛ࠊ2007 ᖺ࠿ࡽ 2010 ᖺ࡟ᤕ⋓ࡉࢀࡓ࢖ࣀࢩࢩࡑࢀࡒࢀ 50 㢌ࠊ71 㢌ࠊ
52 㢌࠿ࡽᚓࡽࢀࡓ⾑Ύࢆ⏝࠸ࡓࠋ
359 ࡟ᑐࡍࡿᢠయࡢ᳨ฟ
࢘࢖ࣝࢫࡣ PRV Indiana ᰴࠊ⣽⬊ࡣࣈࢱ⏤᮶ CPK ⣽⬊㸦ື≀⾨⏕◊✲ᡤࡼࡾศ୚㸧ࢆ⏝࠸࡚୰࿴ヨ㦂࡟ࡼࡾࠊ⾑Ύ୰ࡢ࢘࢖ࣝࢫᢠయࢆ᳨ฟࡋࡓࠋPRV ࡟ᑐࡍࡿᢠయ㝧ᛶಶయࢆ᳨ฟࡍࡿࡓࡵ࡟ࠊ1㸸10 ᕼ㔘⾑Ύࢆ⏝࠸ࡓ 80㸣ࣉ࣮ࣛࢡῶᩘヨ㦂ࢆᐇ᪋ࡋࡓࠋ
㝧ᛶಶయ࡟㛵ࡋ࡚ࡣࠊ⾑Ύࢆ஧ಸ㝵ẁᕼ㔘ࡋ࡚ᢠయ౯ࢆồࡵࡓࠋ࣡ࢡࢳࣥᰴ࡜㔝እᰴࡢ㆑ู
㔝እᰴឤᰁಶయ࡟ࡢࡳฟ⌧ࡍࡿ gE ᢠయࡢ᳨ฟࢆࠊIDEXX ♫ࡢ g1 Antibody ELISAࢆ⏝࠸࡚ᐇ᪋ࡋࡓࠋ᪉ἲࡣ〇ရῧ௜ࡢࣉࣟࢺࢥ࣮ࣝ࡟ᚑࡗࡓࠋ⤖ᯝ࠾ࡼࡧ⪃ᐹ㏆␥ᆅ᪉ࡢ 2 ┴࡛ᤕ⋓ࡉࢀࡓ 6 㢌㸦5%㸧ࡢ࢖ࣀࢩࢩ࡟࠾࠸࡚ࠊPRV ࡟ᑐࡍࡿ࢘࢖ࣝࢫ୰࿴ᢠయ㝧ᛶࡀ☜ㄆࡉࢀࡓࠋ୍᪉ࠊ୰ᅜᆅ᪉ࡢ㸯┴࡛ᤕ⋓ࡉࢀࡓ࢖ࣀࢩࢩ࡟࠾࠸࡚ࡣࠊ රᗜ࣡࢖ࣝࢻࣛ࢖ࣇࣔࣀࢢࣛࣇ 6-8㝧ᛶಶయࡣ☜ㄆࡉࢀ࡞࠿ࡗࡓ㸦⾲ 5㸧ࠋ࢘࢖ࣝࢫ୰࿴ᢠయಖ᭷࡛࠶ࡗࡓ࢖ࣀࢩࢩ 6 㢌ࡣࠊࡍ࡭࡚ gE ࡟ᑐࡍࡿᢠయࢆಖ᭷ࡋ࡚࠸ࡓࠋᚑࡗ࡚࢖ࣀࢩࢩࡣ࣮࢚࢜ࢫ࣮࢟⑓࢘࢖ࣝࢫࡢ㔝⏕ᰴ࡟ឤᰁࡋ࡚࠾ࡾࠊࣈࢱ࡬ࡢឤᰁ※࡜࡞ࡾᚓࡿࡇ࡜ࡀࡀ☜ㄆࡉࢀࡓ㸦ᅗ 4㸧ࠋ㎰ሙෆ࡛ࡢ PRV Ύί໬ࡀᡂ❧ࡋ࡚࠸ࡿሙྜ࡟࠾࠸࡚ࡶࠊ≉࡟࢖ࣀࢩࢩ࠿ࡽࡢ㔝እᰴឤᰁࡢྍ⬟ᛶࢆ⪃៖ࡋࠊᑐ⟇ࢆᐇ᪋ࡍࡿࡇ࡜ࡀᚲせ࡛࠶ࡿࠋ⾲ 㸬࣮࢚࢜ࢫ࣮࢟⑓࢘࢖ࣝࢫ㸦359㸧࡟ᑐࡍࡿᢠయಖ᭷⋡㏆␥ᆅ᪉୰ᅜᆅ᪉

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ㄪᰝᩘᢠయ㝧ᛶᩘᢠయ㝧ᛶ⋡ 㸣 㣴㇜㻟㻢㒔㐨ᗓ┴䛷䝽䜽䝏䞁䝁䞁䝖䝻䞊䝹䛻䜘䜚Ύί໬㽢䞉㣕ἓ䞉᥋ゐ⊟≟䜲䝜䝅䝅⏕⫗䛾ႚ㣗㔝እᰴ䜴䜲䝹䝇䜢ᣢ䛴ྍ⬟ᛶ䛜㧗䛔ᅗ  ࣮࢚࢜ࢫ࣮࢟⑓࢘࢖ࣝࢫ㸦359㸧ࡢឤᰁࡢྍ⬟ᛶ᪥ᮏ⬻⅖᪥ᮏ⬻⅖ࡢ␿Ꮫ࡜୍⯡ᛶ≧᪥ᮏ⬻⅖ࡣࠊ᪥ᮏ⬻⅖࢘࢖ࣝࢫ㸦௨ୗࠊJEV㸧ࡀཎᅉࡢឤᰁ⑕࡛ࠊ့ங㢮࠿ࡽ㫽㢮ࡲ࡛ᗈ⠊࡞ື≀✀࡟ឤᰁࡍࡿࡀࠊ኱㒊ศࡣ୙㢧ᛶឤᰁࢆ♧ࡍࠋࣄࢺ࠿ࡽࣄࢺ࡬ࡢឤᰁࡣ࡞ࡃࠊቑᖜື≀㸨㸦ࣈࢱ㸧ࡢయෆ࡛࠸ࡗࡓࢇቑ࠼࡚⾑ᾮ୰࡟ฟ࡚ࡁࡓ࢘࢖ࣝࢫࢆࠊ⺅ࡀ྾⾑ࡋࠊࡑࡢୖ࡛ࣄࢺ࡞࡝ࢆ่ࡋࡓ᫬࡟ឤᰁࡍࡿࠋࣄࢺࡸ㤿ࡣឤᰁࡍࡿ࡜㔜⠜࡞⬻⅖ࢆⓎ⑕ࡍࡿࡇ࡜ࡀ࠶ࡿࡀࠊᅜෆ࡛ࡣ㏆ᖺ࣡ࢡࢳࣥ᥋✀࡟ࡼࡾࠊࣄࢺࡸ㤿࡛ࡢⓎ⏕ࡣ⃭ῶࡋ࡚࠸ࡿࠋࣈࢱࡣዷፎ㇜࡛ὶ⏘ࢆᘬࡁ㉳ࡇࡍࡇ࡜ࡀ࠶ࡾࠊࡲࡓ㏆ᖺࠊ࢘ࢩ࡛ࡢ JEV ࡟ࡼࡿ⬻⅖ࡢⓎ⏕ࡀᐑᓮ┴ࡸឡ▱┴࡛ሗ࿌ࡉࢀ࡚࠸ࡿ㸦ຍྂ࡯࠿ 2011; ∦ᒣ 2013㸧ࠋ᪥ᮏ⬻ රᗜ࣡࢖ࣝࢻࣛ࢖ࣇࣔࣀࢢࣛࣇ 6-8

⅖ࡣࠊឤᰁ⑕ἲ࡟ᇶ࡙ࡁࠊᝈ⪅ࢆデ᩿ࡋࡓ་ᖌࡣಖ೺ᡤ࡟┤ࡕ࡟ᒆฟࡿࡇ࡜ࡀ⩏ົ࡙ࡅࡽࢀ࡚࠸ࡿࠋ
2008 ᖺ 12 ᭶࡟ࠊරᗜ┴ෆ࡛ᤕ⋓ࡉࢀࡓ࢖ࣀࢩࢩࡢ⾑ᾮ୰࠿ࡽ࢘࢖ࣝࢫࡀศ㞳ࡉࢀࠊ
᭦࡟ 2009 ᖺ 5 ᭶࡟රᗜ┴࡟࠾࠸࡚ᤕ⋓ࡉࢀࡓ࢖ࣀࢩࢩࡢ⾑ᾮ࠿ࡽࡣ࢘࢖ࣝࢫ㑇ఏᏊࡀ᳨ฟࡉࢀࡓ㸦㧗ᓮ࡯࠿ 2009㸧ࠋࡑࡢࡓࡵࠊ᪥ᮏ⬻⅖࢘࢖ࣝࢫࡢឤᰁ⎔࡟࠾࠸࡚ࠊ࢖ࣀࢩࢩࡶቑᖜື≀࡜࡞ࡿྍ⬟ᛶࡀᣦ᦬ࡉࢀ࡚࠸ࡿࠋᮏ◊✲࡛ࡣࠊ㏆␥ᆅ᪉࠾ࡼࡧ୰ᅜᆅ᪉ࡢ
࢖ࣀࢩࢩ࠾ࡼࡧࢩ࢝࡟࠾ࡅࡿ JEV ࡢឤᰁ≧ἣࢆศᯒࡋࡓࠋ
㸨ቑᖜື≀㸸࢘࢖ࣝࢫࢆయෆ࡛ቑᖜࡋࠊ௚ࡢᐟ୺࡟౪⤥ࡍࡿࠋ ᮦᩱ࠾ࡼࡧ᪉ἲ᳨ᰝᑐ㇟ᆅᇦ࠾ࡼࡧࢧࣥࣉࣝᩘ
㏆␥ᆅ᪉࡛ 2009 ᖺ࠿ࡽ 2013 ᖺ࡟ᤕ⋓ࡉࢀࡓ࢖ࣀࢩࢩ 33 㢌ࠊࢩ࢝ 25 㢌ࠊ࠾ࡼࡧ୰
ᅜᆅ᪉࡛ᤕ⋓ࡉࢀࡓ࢖ࣀࢩࢩ 63 㢌ࡼࡾᚓࡓ⾑Ύࢆ⏝࠸ࡓࠋ-(9 ࡟ᑐࡍࡿᢠయࡢ᳨ฟ
JEV/sw/Chiba/88/2002 ᰴࢆ⏝࠸࡚ 80㸣ࣉ࣮ࣛࢡῶᩘヨ㦂࡟ࡼࡾ୰࿴ᢠయ౯ࢆ

ᐃ

ࡋࡓࠋ10 ಸ௨ୖࡢᢠయ౯ࢆᬻᐃⓗ࡟㝧ᛶ࡜ุᐃࡋࡓࠋ
⤖ᯝ࠾ࡼࡧ⪃ᐹ㏆␥ᆅ᪉ࡢ࢖ࣀࢩࢩ 33 㢌୰ 22 㢌㸦67%㸧ࠊࢩ࢝ 25 㢌୰ 23 㢌㸦92%㸧
ࠊ୰ᅜᆅ᪉ࡢ
࢖ࣀࢩࢩ 63 㢌୰ 62 㢌㸦98%㸧ࡀࠊJEV ࡟ᑐࡍࡿᢠయࢆಖ᭷ࡋ࡚࠸ࡓ㸦⾲㸴㸧ࠋ࢖ࣀࢩࢩࡢ᪥ᮏ⬻⅖࡟ᑐࡍࡿᢠయಖ᭷⋡ࡣ㠀ᖖ࡟㧗ࡃࠊࣈࢱ࡜ྠᵝ࡟ቑᖜື≀࡜࡞ࡗ࡚࠸ࡿྍ⬟ᛶࡀ♧၀ࡉࢀࡓࠋࡲࡓࠊࢩ࢝࡟ࡘ࠸࡚ࡶᢠయ㝧ᛶ⋡ࡀ㠀ᖖ࡟㧗࠿ࡗࡓࡓࡵࠊ᪂ࡓ࡞ឤ
ᰁ※࡜ࡋ࡚ὀពࢆࡍࡿᚲせࡀ࠶ࡿ㸦ᅗ㸳㸧ࠋ⾲ 㸬᪥ᮏ⬻⅖࢘࢖ࣝࢫ㸦-(9㸧࡟ᑐࡍࡿᢠయಖ᭷⋡㏆␥ᆅ᪉୰ᅜᆅ᪉࢖ࣀࢩࢩࢩ࢝࢖ࣀࢩࢩㄪᰝᩘᢠయ㝧ᛶᩘᢠయ㝧ᛶ⋡   රᗜ࣡࢖ࣝࢻࣛ࢖ࣇࣔࣀࢢࣛࣇ 6-8ឤᰁ䛩䜛ື≀྾⾑ቑᖜື≀䛸⪃䛘䜙䜜䜛㔝⏕ື≀䞉ᐙ␆྾⾑ᅗ  ᪥ᮏ⬻⅖࢘࢖ࣝࢫ㸦-(9㸧ࡢឤᰁ⎔࡟࠾ࡅࡿ㔝⏕ື≀ࡢᙺ๭ࡲ࡜ࡵᡃࠎࡣࠊ㔝⏕ື≀࡛ࡢ⾑Ύ␿Ꮫㄪᰝࢆᐜ᫆࠿ࡘ≉␗ⓗ࡟ᐇ᪋ࡍࡿࡇ࡜ࢆ┠ⓗ࡜ࡋ࡚ࠊᇵ㣴⣽⬊࡟࡚Ⓨ⌧ࡋࡓࢱࣥࣃࢡ㉁ࢆᢠཎ࡜ࡋ࡚ࠊProteinA/G ࢆ⏝࠸ࡓ ELISA ⣔ࡢᵓ⠏࡟ᡂຌࡋࡓࠋࡉࡽ࡟ᡃࠎࡢ␿Ꮫㄪᰝ࡟ࡼࡾࠊE ᆺ⫢⅖ࠊSFTSࠊ࣮࢚࢜ࢫ࣮࢟⑓ࠊ᪥ᮏ
⬻⅖ࡢཎᅉ࢘࢖ࣝࢫࡣࠊ࢖ࣀࢩࢩࡀಖ᭷ࡋ࡚࠸ࡿྍ⬟ᛶࡀ㧗ࡃࠊࣄࢺ࠾ࡼࡧࣈࢱࢆྵࡴᵝࠎ࡞ື≀࡟ᑐࡋ࡚ࠊ࢖ࣀࢩࢩࡀࡇࢀࡽࡢឤᰁ⑕ࡢឤᰁ※࡜࡞ࡿࡇ࡜ࡀ♧၀ࡉࢀࡓࠋࡲࡓࠊࡇࢀࡽࡢឤᰁ⑕ࡢឤᰁ⤒㊰ࡣࠊᦤ㣗ࠊ྾⾑᪻⹸࡟ࡼࡿ፹௓ࠊ㣕ἓ࡞࡝ᵝࠎ࡛࠶ࡿࠋࡇࢀࡽࡢ⤖ᯝ࠿ࡽࠊ㔝⏕ື≀࡜᥋ゐࡍࡿᶵ఍ࡀከ࠸ࠊ⊁⊟ࢆࡍࡿ᪉ࠎࠊ㔝⏕ື≀ࢆ㣗⫗࡜ࡋ࡚฼⏝ࡉࢀࡿ᪉ࠎࠊ㣴㇜ᴗ⪅ࡢ᪉ࠎ࡟ᑐࡋࠊ௒ᅇㄪᰝࡋࡓឤᰁ⑕ࡢឤᰁࢆண㜵ࡍࡿ᪉ἲ࡜ࡋ࡚ࠊ௨ୗࡢὀព஦㡯ࢆᥦゝࡍࡿࠋ㔝⏕ື≀⟶⌮࡟㛵ࢃࡿ᪉ཬࡧ࢖ࣀࢩࢩࡀ⏕ᜥࡍࡿᆅᇦ࡟࠾ఫ࠸ࡢ᪉࡬‫ࡢ࡝࡞࢝ࢩࡸࢩࢩࣀ࢖ۑ‬㔝⏕ື≀ࢆゎయࡍࡿ㝿ࡣࠊᡭ⿄ࢆ╔⏝ࡋࠊ⾑ᾮ࡟ࡼࡿởᰁࢆ㜵ࡄ‫ࡓࡋ⏝౑ۑ‬ໟ୎ࡸࡲ࡞ᯈ࡞࡝ࡢ㐨ල㢮ࡶࠊᚲࡎ୎ᑀ࡟Ὑίࡍࡿ‫⏕ࠊࡵྵࡶ≟⊟ۑ‬㣗ࡣཝ⚗࡛࠶ࡿࠋႚ㣗᫬࡟ࡣᚲࡎ༑ศຍ⇕ࡍࡿ‫ۑ‬㔝ᒣ࡟⾜ࡃ㝿ࡣࠊ⺅ࡸࢲࢽ࡞࡝ࡢ྾⾑᪻⹸࡟ࡼࡿ྾⾑ࢆண㜵ࡍࡿࡓࡵ࡟ࠊ㛗⿇㺃㛗ࢬ࣎ࣥࡢ╔⏝ࠊ⹸㝖ࡅ࡞࡝ࢆᐇ᪋ࡍࡿ‫࡜ࢱࣈࡣࢩࢩࣀ࢖ۑ‬ඹ㏻ឤᰁࡍࡿ⑓ཎయࢆಖ᭷ࡋ࡚࠸ࡿࡓࡵࠊ㣫⫱ሙෆ࡬ࡢ࢖ࣀࢩࢩࡢ౵ධࢆ㜵Ṇࡍࡿ රᗜ࣡࢖ࣝࢻࣛ࢖ࣇࣔࣀࢢࣛࣇ 6-8ࡲࡓࠊ௒ᅇㄪᰝࡋࡓ࢘࢖ࣝࢫឤᰁ⑕௨እ࡟ࡶࠊ࢖ࣀࢩࢩ➼㔝⏕ື≀ࡀឤᰁ※࡜࡞ࡿឤᰁ⑕ࡀᏑᅾࡍࡿࡇ࡜ࡸࠊᮍࡔⓎぢࡉࢀ࡞࠸࢘࢖ࣝࢫࢆ㔝⏕ື≀ࡀಖ᭷ࡋ࡚࠸ࡿྍ⬟ᛶࡀ࠶ࡿࡇ࡜࠿ࡽࡶࠊୖグࡢ஦㡯࡟ࡣࠊᖖ࡟Ẽࢆ௜ࡅ࡚㡬ࡁࡓ࠸ࠋᘬ⏝ᩥ⊩Hara, Y., Terada, Y., Yonemitsu, K., Shimoda, H., Noguchi, K., Suzuki, K. and Maeda,
K. High prevalence of hepatitis E virus in wild boar in Yamaguchi Prefecture,
Japan. Journal of Wildlife Diseases (In press).
ຍ ྂ ዉ ⥴ ⨾ ࣭ 㕥 ᮌ Ύ ♧ ࣭ ᮡ Ụ ඾ ᫎ 㸬 2011 㸬 ⦾ Ṫ ࿴ ∵ ࡟ ࠾ ࡅ ࡿ ᪥ ᮏ ⬻ ⅖ Ⓨ ⏕ ஦ 㸬
http://www.pref.aichi.jp/cmsfiles/contents/0000049/49901/13.pdf
2014.3.10 accessed.
∦ᒣ㈗ᚿ㸬2013㸬Ꮚ∵ࡢ኱⬻࡟࠾ࡅࡿ᪥ᮏ⬻⅖࢘࢖ࣝࢫ࡟ࡼࡿ㠀໬⮋ᛶ㧊⭷⬻⅖. ື⾨◊◊✲ሗ࿌. 119:37-45.
ᖾ⏣▱Ꮚ࣭ᰗᮏ῟Ꮚ࣭᯽ཎ ⿱࣭௚㸬2000㸬≉㞟 㔝⏕ື≀ࡢឤᰁ⑕㸦2㸧㔝⏕࢖ࣀࢩࢩࡢ࣮࢚࢜ࢫ࣮࢟⑓㸬㸬⋇་␆⏘᪂ሗ. 53(11)㸹939-943㸬
๓⏣ ೺㸬2014㸬㔜⑕⇕ᛶ⾑ᑠᯈῶᑡ⑕ೃ⩌㸦SFTS㸧࢘࢖ࣝࢫࡢศ㞳࠿ࡽ᭱᪂ࡢ▱ぢࡲ࡛.
໬Ꮫ⒪ἲࡢ㡿ᇦ. 30(2):75-88㸬
Mahmoud, HYA., Suzuki, K., Tsuji, T., Yokoyama, M., Shimojima, M. and Maeda K. 2011.
Pseudorabies virus infection in wild boars in Japan. Journal of Veterinary MedicalScience 73(11): 1535-1537.
᳃ᕝ ⱱ࣭Ᏹ⏣ᬗᙪ࣭ຍ᮶⩏ᾈ࣭ᮌᮧᫀఙ࣭௒ᒸᾈ୍࣭⚟ኈ⚽ᝋ࣭ྜྷἙᬛᇛ࣭㇂ⱥᶞ࣭ୗᓥᫀᖾ࣭Ᏻ⸨⚽஧࣭すᲄᨻᖾ࣭⃝㎶ிᏊ࣭ᕝ➃ᐶᶞ࣭᪂಴ ⥤࣭๓⏣ ೺࣭㧗㔝 ឡ࣭ᰗ஭ᚨ☻࣭⸨⏣༤ᕫ࣭㧗⏣ఙᘯ㸬2013㸬㸺㏿ሗ㸼㔜⑕⇕ᛶ⾑ᑠᯈῶᑡ⑕ೃ⩌㸦SFTS㸧࢘࢖ࣝࢫࡢᅜෆศᕸㄪᰝ⤖ᯝ㸦➨୍ሗ㸧㸬ᅜ❧ឤᰁ⑕◊✲ᡤ㸪⑓ཎᚤ⏕≀᳨ฟ᝟ሗ㸬
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