Instruction Manual

ZEISS Axiocam 105 color
Version R2

ZEISS Axiocam 105 color
Original Manual
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena
Germany
microscopy@zeiss.com
www.zeiss.com/microscopy

Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena
Germany

Document Name: Instruction Manual ZEISS Axiocam 105 color
Order Number: 426555-7010-001
Revision: 2
Language: en-US
Effective from: 09/2022© 2022 Without the prior written consent of ZEISS, this document or any part of it must neither be translated nor
reproduced or transmitted in any form or by any means - including electronic or mechanic methods, by photocopying, recording or by any information or filing system. The right to make backup-copies for archiving purposes shallremain unaffected thereby. Any violations may be prosecuted as copyright infringements.
The use of general descriptive names, registered names, trademarks, etc. in this document does not imply thatsuch names are exempt from the relevant intellectual property laws and regulations and therefore free for generaluse. This shall also apply if this is not specifically referred to. Software programs shall entirely remain the propertyof ZEISS. No program or subsequent upgrade thereof may be disclosed to any third party, copied or reproduced inany other form without the prior written consent of ZEISS, even if these copies or reproductions are destined forinternal use at the customer's only, the only exception being one single back-up copy for archiving purposes.

Content​

ZEISSContent
1 About this Instruction Manual.......................................................

5

1.1

Introduction ...............................................................................................

5

1.2

Text Conventions and Link Types................................................................

5

1.3

Explanation of Warning Messages and Additional Information ...................

6

2 Safety ............................................................................................

8

2.1

Intended Purpose .......................................................................................

8

2.2

General Safety Information.........................................................................

8

2.2.1
2.2.2

Requirements for Operators........................................................
Safe Operating Condition ...........................................................

8
8

Prevention of Hazards ................................................................................

9

2.3

3 Technical Data and Conformity ..................................................... 10
3.1

Axiocam 105 color R2 ................................................................................ 10
3.1.1

3.2

Spectral Sensitivity ...................................................................... 11

Applicable Standards and Regulations ........................................................ 11

4 Shipment ....................................................................................... 12
5 Connecting the Camera ................................................................. 13
5.1

Installing the interface card ........................................................................ 13

5.2

Mounting the camera on the microsope..................................................... 13

5.3

Connecting the camera with PC ................................................................. 13

5.4

Function indicator ...................................................................................... 14

6 Installing Software and Drivers ..................................................... 15
7 Care and Maintenance................................................................... 16
7.1

Optical System ........................................................................................... 16

7.2

Cleaning the infrared filter or protective glass............................................. 16

8 Troubleshooting ............................................................................ 17
8.1

The camera does not appear in the menu of selectable cameras ................ 178.2

You don't see a camera image on your screen ........................................... 17

8.3

The color of my image does not correspond to the impression through theocular......................................................................................................... 17

8.4

The performance of the camera is not consistent or is too slow ................. 17

Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001

3

Content​

ZEISS

9 Disposal......................................................................................... 18

Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001

41 About this Instruction Manual | 1.1 Introduction

ZEISS

1 About this Instruction ManualThis Instruction Manual (further called "document") is considered to be part of the microscopecamera, herein after referred to as the "product" or the "camera".
This document contains basic steps and safety information that must be observed during operation and maintenance. Therefore, the document must be read by the operator prior to commissioning and must always be available at the place of use of the product.
This document is an essential part of the product and, if the product is resold, the document mustremain with the product or be handed over to the new owner.

1.1 Introduction
This camera is a high-resolution digital microscope camera with a color CMOS sensor and USB 3.0
interface for light microscopy applications. To make it easier for you to set up the camera, follow
the instructions in these chapters step by step. In this manual the interface between the camera
and the computer is called USB 3.0.
Content This document contains all the information you need to set up the microscope, start the software,and perform troubleshooting and maintenance. It contains the following chapters:

ChapterContent

About this Instruction Manual

Introduction and overview about this manual.

SafetyImportant information on safe handling with the camera.
Read this chapter before unpacking the camera andputting it into operation.

Technical Data and Conformity

Here you will find the camera's technical data.

Shipment

The contents of delivery and optional attachments will be
described here.

Connecting the Camera

In this chapter you will find detailed instructions on connecting and using the camera.

Installing Software and Drivers

Here you will learn how to install ZEISS software and camera drivers.

Care and MaintenanceThis chapter describes some measures for the maintenanceand care of your camera. In case of greater damage, always
contact ZEISS support.

Troubleshooting

In this chapter we have listed some solutions to various
problems. If you can not solve your problem, contact ZEISS
support.

Disposal

Information on the disposal of the product.

1.2 Text Conventions and Link Types
ExampleExplanation
The names of controls and important information are shown in bold letters, for instance:

Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001

51 About this Instruction Manual | 1.3 Explanation of Warning Messages and Additional Information

ZEISS

ExampleExplanation

Click Start.

Software controls and GUI elements.

Press the Standby button.

Hardware controls and elements.

Press Enter on the keyboard.

Key on the keyboard.

Press Ctrl + Alt + Del.

Press several keys on the keyboard simultaneously.

Select Tools > Goto Control Panel > Airlock.

Follow a path in the software.

Enter example.pdf in this field.

Text to be entered by the user.

Programming and Macros

Anything typed in literally during programming, including, for example, macro codes,
keywords, data types, method names, variables, class names, and interface names.

Tab. 1: Text conventions

ExampleExplanation

See: Text Conventions and Link Types [} 5].

Link to further information for this topic.

https://www.zeiss.com/corporate/int/
home.html

Link to a website on the internet.

Tab. 2: Link types

1.3 Explanation of Warning Messages and Additional Information
CAUTION, and NOTICE are standard signal words used to determine the levels of hazards andrisks of personal injury and property damage. Not only the safety and warning messages in the
Safety chapter are to be considered also all safety and warning messages in other chapters. Failure to comply with these instructions and warnings can result in both personal injury and property
damage and involve the loss of any claims for damages.
The following warning messages indicating dangerous situations and hazards are used in this document.

CAUTION
Type and source of danger
CAUTION indicates a potentially hazardous situation which, if not avoided, may result in minor
or moderate injury.

NOTICE
Type and source of danger
NOTICE indicates a potentially harmful situation which, if not avoided, may result in property
damage. In addition, NOTICE warns of data loss or corrupt data as well.

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6

1 About this Instruction Manual | 1.3 Explanation of Warning Messages and Additional Information

ZEISS

Info
Provides additional information or explanations to help operator better understand the contents of this document.

Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001

72 Safety | 2.1 Intended Purpose

ZEISS

2 Safety
2.1 Intended Purpose
The product is a high definition microscope camera for color imaging. It is suitable for use as anaccessory for educational and routine microscopy in laboratory environments and for use bytrained laboratory personnel. The camera has been designed to be used in the field of light microscopy for general observation, routine work, and simple applications in which a sufficientamount of light is available.
The camera should only be used for training and research. The images / videos from this camera
must not be used for the direct generation of diagnostic results.

2.2 General Safety Information
This document must be read before commissioning in order to ensure safe and uninterrupted operation. Pay particular attention to all listed safety notes. Make sure, that§the operating personnel has read and understood this manual, associated documents, andparticularly all safety regulations and instructions, and applies them.

§

the local and national safety and accident prevention regulations must be observed, as well as
the applicable laws and regulations in your country.

§

this document is always available at the place of use of the product.

§

the product is always in perfect condition.

§

the product is secured against access by unauthorized persons.

§maintenance and repair work, retrofitting, removal or replacement of components, as well as
any other intervention in the product not described in this document, may only be carried out
by the manufacturer ZEISS or persons expressly authorized by ZEISS to do so.

2.2.1 Requirements for Operators
The product, components, and accessories may only be operated and maintained by authorized
and trained personnel. The product may only be used in accordance with this document. If the
product is not used as described, the safety of the user may be impaired and/or the product may
be damaged.
Any unauthorized intervention or use other than within the scope of the intended use shall void
all rights to warranty claims. The regional regulations on health protection and accident prevention must be observed at all times and during all work on and with the product.

2.2.2 Safe Operating Condition
If circumstances occur which impair safety and cause changes in operating behavior, the productmust be shut down immediately and a ZEISS service representative should be informed.
The product may only be operated if the operating conditions are adhered to.
§

Do not operate the product until you have completely read and understood the entire documentation.

§

Ensure conditions and take measures to prevent the build up of electrostatic charge on theworkplace.

Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001

82 Safety | 2.3 Prevention of Hazards

ZEISS

2.3 Prevention of Hazards
This product has been manufactured and tested by ZEISS according to the regulations specified in
CE and has left the manufacturer’s premises in perfect working order. The camera is intended to
be used in a basic electromagnetic environment while the noise emission conforms to CISPR 11
Class B level. The EMC performance might be impaired while integrated into a system. In order toensure that this condition is maintained and to avoid any risks when operating the system, theuser must comply with any notes and warnings contained in this manual. The manufacturer shallbe exempt from statutory liability for accidents should the operator fail to observe the safety regulations.

CAUTION
Personal Injury
To avoid personal injury, read and adhere to the safety notes below.
4 To avoid the risk of fire or explosion, do not use the camera near inflammable liquids orgases.
4 Setup, expansions, re-adjustments, alterations, and repairs must be carried out only by
persons who have been authorized by ZEISS.
4 Do not allow any cables, particularly power cords, to trail across the floor, where they canbe snagged by people walking past.
4 Protect the cables from excessive heat (e.g. halogen lamps, microscope fluorescence illumination).
4 To avoid injuries due to potentially high surface temperatures, do not touch the camera's
surface for a prolonged time.
4 Do not position the equipment in a way that makes it difficult to operate or disconnect thedevice.

NOTICETo avoid equipment damage, data loss, or corrupted data, read and adhere to the safety notes
below.
4 Protect the camera against mechanical impact. External damage may affect the operationof inner components.
4 Keep chemicals and fluids away from the camera.
4 Make sure there is sufficient ventilation of the camera head. Avoid direct exposure to sunlight and locations near heat sources (radiators, stoves). Overheating can cause noisy images.
4 Use the camera in a clean and dry location.
4 Attach all connectors firmly and securely.
4 Use only the accessories supplied by ZEISS, when applicable.
4 Use only normal microscope cleaning material to clean the camera housing.
4 Contact your local ZEISS service organization if a repair is necessary. Do not disassemble
the camera by yourself, otherwise the warranty will be lost.
4 Save all your data, such as images, measurement data, archives, reports, forms and documents, at regular intervals on an external storage medium. Otherwise it cannot be avoided
that access to this data may be lost as a result of operational errors or hardware defects.
ZEISS accepts no liability for consequential damage resulting from insufficient data protection.

Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001

93 Technical Data and Conformity | 3.1 Axiocam 105 color R2

ZEISS

3 Technical Data and Conformity
3.1 Axiocam 105 color R2
FeatureValue

Sensor TypeCMOS color sensor

Number of Pixels

2592 px x 1944 px (horizontal x vertical) = 5 megapixels

Pixel Size

2.2 μm x 2.2 μm

Chip Size

5.70 mm x 4.28 mm, corresponds to 1/2.24"

Live Image Frame Rate

2592 px x 1944 px (horizontal x vertical)
Frame rate @1 ms: 30 fps

Sensor Subarea Readout(ROI)

Freely adjustable
Min. size: 864 px x 1 px (horizontal x vertical)

Digitization

8 Bit / pixel

Exposure Time

30 μs up to 1 s

Interface (camera)

USB 3.0 Micro-B

Interface (PC / card)

USB 3.0 Standard-A

Optical Interface

C-Mount adapter

Dimensions

29 mm x 29 mm x 17.0 mm (height x width x depth)

Operating System

Microsoft® Windows 10

Certificates

CE, FCC Class B, RoHS

Power Supply

Via USB 3.0 interface

USB Power

5 V, max. 400 mA

Environment Conditions

0° ... + 50° Celsius, max. 80% relative air humidity, no condensation, free air circulation required

Order Number

426555-9010-000

Info
Computer hardware, operating system and software may decrease the frame rates. Selecting apart of the sensor area or applying binning can increase the frame rate. All specifications are
subject to change without notice.

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3 Technical Data and Conformity | 3.2 Applicable Standards and Regulations

ZEISS

3.1.1 Spectral Sensitivity

Fig. 1: Spectral Sensitivity Axiocam 105 color R2

3.2 Applicable Standards and Regulations
Observe all general and country-specific safety regulations as well as applicable environmental
protection laws and regulations. This product is in compliance with the requirements of the following regulations and directives:
2014/30/EU

Electromagnetic Compatibility

2011/65/EU

and amendment directive (EU) 2015/863 - RoHS Restriction of the use of certain hazardous substances inelectrical and electronic equipment

CE

The product fulfills the standards and regulations mentioned
above and is compliant to the CE guideline

For more information on CE Declarations of Conformity, contact your ZEISS Sales & Service Partner.

Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001

114 Shipment​

ZEISS

4 Shipment
Content

Ord. no1 x Axiocam 105 color R2 camera

426555-9010-000

1 x USB 3.0 data cable
1 x Download information for ZEISS software and instruction
manualOptional accessories (recommended): PCI express interface card

Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001

000000-0591-463

12

5 Connecting the Camera | 5.1 Installing the interface card

ZEISS

5 Connecting the Camera
5.1 Installing the interface card
This chapter describes how to install the PCI express interface card (2 x USB 3.0) into your PC.

NOTICE
Static Electricity
Static electricity can damage electronic components. To protect electronic components against
static electricity, do not touch them until you have grounded yourself to the casing of the device. Never touch the contacts of the electronic components. We also recommend that you
work only on an antistatic mat.
Prerequisite ü You have read the documentation of the interface card and of your computer before inserting
the interface card.
1.
2.
3.
4.

Switch off your PC and all connected peripherals.
Disconnect the PC and the peripherals from the mains and open the computer case.
Insert the PCIe interface card into the appropriate slot on your computer.
Close the computer case and reconnect the peripherals.

You can now mount the camera on the microscope and connect it to the PC, see chapter Connecting the camera to the PC.

5.2 Mounting the camera on the microsope
To mount the camera onto your microscope’s camera port, use a C-mount adapter. NOTICE! The
adapter is not included in the scope of delivery. You will find some suitable examples foradapters in the list below:

Camera Adapter

Description

Camera Adapter 60N-C 2/3" 0.5x426112-0000-000

Camera Adapter 60N-C 2/3" 0.63x

426113-0000-000

Camera Adapter 95P-C 1/2" 0.5x

415501-1811-000

Camera Adapter 95P-C 2/3" 0.65x

415501-1810-000

1.
2.
3.
4.

Remove the dust cap from the camera's C-mount port.
Mount the C-mount camera adapter to the camera.
Attach the camera with the adapter to the microscope's 60N (P95) port.
Orient the camera to the stand and fix its position by tightening the ring nut.

5.3 Connecting the camera with PC
1. Lay the supplied USB 3.0 cable carefully between the camera and the PC.
NOTICE! Make sure that the cable is in a safe distance from hot light sources on
the microscope, to prevent damage from heat.
2. Connect the USB 3.0 cable to the camera. Tighten the screws with a screwdriver until it isfinger tight.

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13

5 Connecting the Camera | 5.4 Function indicator

ZEISS

3. Connect the other end of the USB 3.0 cable to the USB 3.0 interface of the main board(and not USB 2.0) or the recommened USB 3.0 PCIe interface card.
4. Switch on your PC if its not switched on already. The camera is ready for use.

Info
Power is supplied to the camera via the USB 3.0 interface. If you switch off the PC, the camera
is also switched off.

5.4 Function indicator
After you have switched on the PC, the LED on the camera will light up red. As a rule, if the LED isred, this indicates that power is being properly supplied to the camera via the data cable. However the driver has not yet been installed. For the definitions of the LED color signals, refer to thelist below:

SignalMeaning

Red

Power supplied; no driver loaded, camera not detectedGreen flash 3x

USB 3.0 connection detected

Green

Driver loaded; camera readyOffNo power supplied

Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001

146 Installing Software and Drivers​

ZEISS

6 Installing Software and Drivers
Prerequisite ü To install the software for image acquisition, you must install the ZEISS Microscopy Installer.
The camera drivers will be installed automatically during the installation of the software.
ü Before starting the installation of the software and drivers, the camera must be connected to
the PC.
1. Install the software according to the installation guide delivered with the software.
2. During the installation you will be asked to install the camera drivers. Follow the procedureby confirming the upcoming messages.
3. Restart your PC after the installation.
4. You can check in the device manager if the driver installation was successful.
You can now start acquiring images with your camera using ZEISS software. For more information, please read the software documentation.

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7 Care and Maintenance | 7.1 Optical System

ZEISS

7 Care and Maintenance
7.1 Optical System
The internal optical components of the camera should always be protected. If no lens, or camera
adapter with optics, is screwed into the camera's C-Mount thread, the camera's sensor and protective glass must be protected by screwing the protective cap onto the camera's C-Mountthread.

7.2 Cleaning the infrared filter or protective glass
Contamination of the IR filter (for color cameras only) or the protective glass has an adverse effecton the quality of the resulting image (dark points, cloudy structures in the image). If there is dry
dust on the front side of the infrared filter or protective glass, you can clean it with a soft brush orwith cotton (wool). Use cleaning fluid for optics/lenses only to clean the IR filter. Do not use tap
water to clean the IR filter.

Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001

168 Troubleshooting | 8.1 The camera does not appear in the menu of selectable cameras

ZEISS

8 Troubleshooting
8.1 The camera does not appear in the menu of selectable cameras§

Make sure that you have connected the camera and installed the software and drivers in accordance to the instructions in this manual.

§

Make sure that you installed the software and drivers with administrative rights. Install thedriver with administrative rights again if necessary.

8.2 You don't see a camera image on your screen§

Check if you have started ZEN and clicked the live image button.

§

Check the light path setting of the microscope.

§Is the status LED on the camera on? If not, check the cable connections between camera andcomputer.

§

Reset the camera parameters to default values.

§

Execute an automatic exposure measurement.

§

Check the display adjustments for the live image.

§Check the aperture diaphragm of the microscope.

§

Check the position of the beam splitter between the ocular and the camera port.

For operating the camera with the software, see ZEN Help. Press the F1 key to access the contextsensitive ZEN Help.

8.3 The color of my image does not correspond to the impression through theocularIn case of color cameras:§

Check white balance and, if necessary, repeat white balance.

§

Check the monitor’s color temperature setting. If necessary, reduce this to the lowest valuethat can be set (usually 5200 K).

In case of monochrome cameras:§

Images of monochrome cameras are displayed by using overlay colors to represent the colorsof fluorescence dyes on the monitor. If the color is different to the visual impression in theeyepiece, select a different overlay color.

8.4 The performance of the camera is not consistent or is too slow§

Generally the performance of the camera is dependent on the performance of the PC. Make
sure the PC hardware and setup are optimal for large data transfers.

§

Check that the camera is connected to the USB 3.0 interface of the main board (and not USB2.0) or the recommened USB 3.0 interface card on the PC.

§

Make sure no other devices are plugged into the USB 3.0 interface card. This can potentiallyreduce the speed of the USB 3.0 port.

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9 Disposal​

ZEISS

9 Disposal
The product must not be disposed of as domestic waste or through municipal disposal companies. They must be disposed of in accordance with applicable regulations (WEEE Directive
2012/19/EU). ZEISS has implemented a system for the return and recycling of devices in member
states of the European Union that ensures suitable reuse according to the EU Directives mentioned. The customer is responsible for decontamination.

Info
Detailed information on disposal and recycling is available from your ZEISS Sales & Service Partner.

Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 | 426555-7010-001

18Instruction Manual ZEISS Axiocam 105 color | en-US | Rev.2 |Modifications reserved.
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena
Germany

phone: +49 3641 64 3161
fax: +49 3641 64 3439
info.microscopy@zeiss.com
www.zeiss.com/microscopy