チーム名：ライブセル分子イメージング研究チーム（１） 原著論文 ／ Original Papers
1. T. Uemura, Y. Suda, T. Ueda, and A. Nakano: “Dynamic behavior of the trans-Golgi networkin root tissues of Arabidopsis revealed by super-resolution live imaging,” Plant Cell
Physiol. 55, 694-670 (2014).
2. Y. Hashiguchi, D. Yano, K. Nagafusa, T. Kato, C. Saito, T. Uemura, T. Ueda, A. Nakano,
M. Tasaka, and M. T. Morita: “A unique HEAT repeat-containing protein SHOOTGRAVITROPISM 6 is involved in vacuolar membrane dynamics in gravity sensing cells ofArabidopsis inflorescence stem,” Plant Cell Physiol. 55, 811-822 (2014).
3. M. Fujiwara, Uemura, K. Ebine, Y. Nishimori, T. Ueda, A. Nakano, M. H. Sato, and Y.
Fukao: “Interactomics of Qa-SNARE in Arabidopsis thaliana,” Plant Cell Physiol. 55,
781-789 (2014).
4. H. Kawai-Toyooka, T. Mori, T. Hamaji, M. Suzuki, B. J.
Ueda,A.

Nakano,A.

Toyoda,A.

Fujiyama,andS.

C. Olson, T. Uemura, T.

H.

Nozaki:“Sex-specificpost-translational regulation of the gamete fusogen GCS1 in the isogamous volvocine
alga Gonium pectoral,” Eukaryotic Cell 5, 648-656 (2014).
5. K. Kurokawa, M. Okamoto, and A. Nakano: “Contact of cis-Golgi with ER exit sites
executes cargo capture and delivery from the ER,” Nat. Commun. 5, 3653 (2014).
6. T. Haraguchi, M. Tominaga, R. Matsumoto, K. Sato, A. Nakano, K. Yamamoto, and K. Ito:
“Molecular characterization and subcellular localization of Arabidopsis class VIII
myosin ATM1,” J. Biol. Chem. 289, 12343-12355 (2014).
7. S. Miyagishima, T. Fujiwara, N. Sumiya, S. Hirooka, A. Nakano, Y. Kabeya, and M.
Nakamura: “Translation-independent circadian control of the cell cycle in aunicellular photosynthetic eukaryote,” Nat. Commun. 5, 3807 (2014).
8. Y. Cui, Q. Zhao, C. Gao, Y. Ding, Y. Zeng, T. Ueda, A. Nakano, and L. Jiang: “Activationof the Rab7 GTPase by the MON1-CCZ1 complex is essential for PVC-to-vacuole trafficking
and plant growth in Arabidopsis,” Plant Cell 26, 2080-2097 (2014).
9. K. Ebine, T. Inoue, J. Ito, E. Ito, T. Uemura, T. Goh, H. Abe, K. Sato, A. Nakano, andT. Ueda: “Plant vacuolar trafficking occurs through distinctly regulated pathways,”
Curr. Biol. 24, 1375-1382 (2014).
10. S. Naramoto, M. S. Otegui, N. Kutsuna, R. de Rycke, T. Dainobu, M. Karampelias, M.
Fujimoto, E. Feraru, D. Miki, H. Fukuda, A. Nakano, and J. Friml: Insights into thelocalization and function of the membrane trafficking regulator GNOM ARF-GEF at theGolgi apparatus in Arabidopsis,” Plant Cell 26, 3062-3076 (2014).
11. M. Iwai, M. Yokono, M. Kono, K. Noguchi, S. Akimoto, and A. Nakano: “Light-harvesting
complex Lhcb9 confers a green alga-type photosystem I supercomplex to the moss

Physcomitrella patens,” Nat. Plants 1, 14008 (2015).

12. M. Fujimoto, Y. Suda, S. Vernhettes, A. Nakano, and T. Ueda: “Phosphoinositol 3-kinase
and 4-kinase have distinct roles in intracellular trafficking of the cellulose synthase
complex in Arabidopsis thaliana,” Plant Cell Physiol. 56:287-298 (2015).
13. T. Tsutsui, A. Nakano, and T. Ueda: "The plant-specific RAB5 GTPase ARA6 is requiredfor starch and sugar homeostasis in Arabidopsis thaliana," Plant Cell Physiol. 56,1073-1083 (2015).
14. M. Iwai, M. Yokono, and A. Nakano: “Toward understanding the multiple spatiotemporal
dynamics of chlorophyll fluorescence,” Plant Signal. Behav. in press.
15. K. Nakanishi, K. Kakiguchi, S. Yonemura, A. Nakano, and N. Morishima: "Transient Ca2+
depletion from the endoplasmic reticulum is critical for skeletal myoblast
diffrentiation," FASEB J. 29, 2137-2149 (2015).
16. A. Baral, N. G Irani, M. Fujimoto, A Nakano, S. Mayor and M. K. Mathew: "Salt inducedremodelling of spatially restricted clathrin-independent endocytic pathways in
Arabidopsis root," Plant Cell 27, 1297-1315 (2015).
17. H. Shinohara, M. Behar, K. Inoue, M. Hiroshima, T. Yasusda, T. Nagashima, S. Kimura,
H. Sanjo, S. Maeda, N. Yumoto, S. Ki, S. Akira, Y. Sako, A. Hoffman, T. Kurosaki, andM. Okada-Hatakeyama: “Positive feedback within a kinase signaling complex functions
as a switch mechanism for NF-kB activation,” Science, 344, 760-764 (2014).
18. S.-i. Morita, S. Takanezawa, M. Hiroshima, T. Mitsui, Y. Ozaki, and Y. Sako: “Ramanand autofluorescence spectrum dynamics along the HRG-induced differentiation pathwayof MCF-7 cells,” Biophys. J. 107, 2221-2229 (2014).
19. H. Park, S.-S. Han, Y. Sako, and C.-G. Pack: “Dynamic and unique nucleolar
microenvironment revealed by fluorescence correlation spectroscopy,” FASEB J. 29,837-848 (2015).
20. Y. Arata, H. Takagi, Y. Sako, and H. Sawa: “Power law relationship between cell cycle
duration and cell volume in the early embryonic development of Caenorhabditis elegans,”Frontiers in Physiol. DOI: 10.3389/fphys.2014.00529 (1-11) (2015).
21. S. Takanezawa, S.-i. Morita, Y. Ozaki, and Y. Sako: “Raman spectral dynamics of single
cells in the early stages of growth factor stimulation,” Biophys. J. 108, 2148-2157(2015).

（２） 著書・解説など ／ Book Editions, Review Papers
1. Y. Ito, T. Uemura, and A. Nakano: “Formation and maintenance of the plant Golgi
apparatus,” Intl. Rev. Cell Mol. Biol. 310, 221-287 (2014).
2. A. Nakano: “Cell biology: Polarized transport in the Golgi apparatus,” Nature 28,427-428 (2015).

3. D. G. Robinson, F. Brandizzi, C. Hawes, and A. Nakano: “Vesicles versus tubes: is ER-Golgi
transport in plants fundamentally different from other eukaryotes?” Plant Physiol.
168, 393-406 (2015).
4. C.-G. Pack, M.-K. Jung, M.-R. Song, J.-S. Kim, S.-S. Han, and Y. Sako: “Use ofengineered nanoparticle-based fluorescence methods for live-cell phenomena,“ in“Fluorescence Microscopy: Super-Resolution and Other Novel Techniques.” pp.153-170.
Cornea, A. and Conn, P. M. eds. Elsevier (2014).

（３） 招待講演 ／ Invited Talks
1. A. Nakano: “Development of super-resolution live imaging microscopy (SCLIM) to tackle
molecular mechanisms of membrane trafficking,” International ERATO Higashiyama
Live-Holonics Symposium, Nagoya, Japan, September 10 (2014).
2. R. Maeda, M. Hiroshima, T. Yamashita, A. Wada, S. Nishimura, Y. Sako, and Y. Shichida:“Single-molecule observation of the ligand-induced population shift of rhodopsin, aG-protein coupled receptor,” Single Protein Dynamics in Cellulo SPDC 2014, Onna,Okinawa, Japan, April 24 (2014).
3. Y. Sako: “Single-molecule analysis of cell signaling reactions,” Single Protein
Dynamics in Cellulo SPDC 2014, Onna, Okinawa, Japan, April 25 (2014).
4. Y. Sako: “Experimental aspects of intracellular dynamics of cell fate decision,” The
Joint Annual Meeting of The Japanese Society for Mathematical Biology and The Societyfor Mathematical Biology 2014, Osaka, Japan, July 30 (2014).
5. M. Hiroshima, and Y. Sako: “Regulation of Cellular Signaling by ErbB System,“ TheJoint Annual Meeting of the Japanese Society for Mathematical Biology and the Society
for Mathematical Biology 2014, Osaka, Japan, July 30 (2014).

（４） 特許出願 ／ Patent Applications
1. 中野明彦，市原昭，“対物レンズの駆動制御方法及び蛍光顕微鏡システム，” 国際出願番号
PCT/JP2014/00434，書類番号 24779，2014 年 8 月 25 日．