Easy evaluation using Mylc is now available anytime, anywhere.

Evaluation kit[Mylc ELISA (human IL-6) Kit]

You can obtain the results the day following the evaluation.
This speed greatly accelerates research and development.
Evaluation kit using Mylc cells, which are excellent in precision and reproducibility. This kit contains the key materials
and the evaluation conditions have already been optimized. Evaluation using cells which you are not familiar with is often troublesome,
but you can start evaluation quickly with this kit optimized for evaluation. The result can be obtained in only 24 hours after adding
the test substances. This kit allows you to reduce time and cost of evaluation drastically.

Mylc ELISA( human IL-6 )kit

Condition setting has already been done.
You can start evaluation studies immediately.
Mylc cells

Measurement
reagents

POINT

1

POINT

2

POINT

3

Specific culture medium

You can readily perform
high quality evaluation.
You can readily use a large quantity of homogeneous Mylc
cells for evaluation without troublesome condition setting.

Free from complicated condition setting.
Because the kit is delivered with the optimum conditions
for evaluation, you can readily start evaluation.

You can start immediately
with this All-in-One kit.
This kit contains all the materials necessary for evaluation:from blood cells to measurement reagents. You can startevaluation immediately after opening the box.

Principle of evaluation
A test substance is added to theMylc cells, and the produced
transmitter (IL-6) is quantified.
In 24 hours, the intensity of thestimulation by the test substancecan be evaluated numerically, whichenables judgment of the function.

When Mylc cells ( immature dendritic cells ) are stimulated by test substances,various substances such as IL-6 are produced.

Mylc cells

Release transmittersin response to test substances.

• Test substance• Stimulant
Addition

Dendritic cell( Immature )Quantitative
measurement
Culture for
24 hDendritic cells(mature)Transmitter( IL-6, etc.)

Differentiation and maturation of myeloid cells
iPS/ES cell

CFU-GM

CFU-M

MonoblastWhite blood cell(Monocytes)

Dendritic cell ( Immature )Mylc
Macrophage

Dendritic cell ( Antigen presenting cell )StimulationT cell

Transmitter

Optimum differentiation
state for evaluation

Homogeneous blood cellswith identical genetic information

We can control the differentiation
stage to the optimum conditions

Stable mass-production
and supply are possible

The same genetic information and conditions can bealways reproduced because they are produced in ourlaborator y. This feature contributes to a drastic
improvement in the accuracy and applicabilit y ofpharmaceutical evaluation.

Dif ferentiation stage (maturit y) can be minutely
controlled with our high level of regenerative medicine
technologies and product control. Research andevaluation of high accuracy are available.

You can obtain blood cells in the sufficient quantities
in ideal condition any time. You can conduct researchand development and efficacy evaluation with nodelay and accelerate your development.

Assay procedureThawing ofMylc cells

Addition oftest substanceSeeding of cells

Transfer ofculture supernatant

Measuredwith ELISA kit

to 96-well culture platein the kit

ELISA kit

Specific culture medium

Thawing → Suspendedin culture medium
Cell suspension

Mylc cells

STEPCulture for 24 h1

STEP

96-well culture plate

2

STEP3STEP

4STEP

5STEP

6

Thawing ofMylc cells

Preparation of
Mylc cells

Seeding ofcells

Addition oftest substanceCollection of
Measurement of IL-6
culture supernatant in culture medium

Frozen cells in a vial arethawed in a water bath.

After washing by
centrifugation, the cells
are suspended in a
specific culture medium.

Cells are seeded at a
certain density in
96-well culture plates
you provide.

Add test substancewith the stimulant,
and culture for 24 h.

Transfer culture
supernatant from eachwell to the 96-wellculture plate in the kit.

Quantified in acolorimetric ELISA assay
according to the method
attached to the kit.

In evaluation based on alternative methods to animal testing,
Mylc cells respond with high sensitivity and stability.
Mylc, which shows similar response to in vivo cells, is superior in sensitivity in sensitization and stability comparedwith model cells (Mono-Mac-6, etc. ) used in alternative methods to animal testing. In a comparison study in IL-6 production assay,
Mylc-02 (right figure), used in this study, shows higher response at lower concentrations than Mono-Mac-6 (left figure ).

Comparison study with the Mono-Mac-6
Comparison study in IL-6 production assay, in which a pyrogen ( LPS) was added (expressed in OD)
In Mylc-2 (right figure), used in this study, the production was detectable at lower concentrations than in Mono-Mac-6 (left figure), indicating a higher response.

OD(IL-6)
3.0

OD(IL-6)
3.0

THP-1

2.5

iMylc-2

2.5

Mono-Mac-6

2.0

2.0

1.5

1.5

1.0

1.0

0.5

0.5

0.0

0.0
0.00010.001

0.1101000100000
LPS(pg/mL)

0.00010.001

0.1101000100000
LPS(pg/mL)

Contents of kit*
Cells : Mylc cells (frozen in storage medium, 1 screw vial)* Please provide the following instruments and materials by yourself in advance.

Specific culture medium : Specific for culture of Mylc cells for measurement and evaluation(frozen product, 50 mL bottle × 1)

Sterilized centrifuge tubes / Pipettes and pipette tips / 96-well culture plate
Centrifuge, CO 2 incubator / other necessary materials and instruments

Stimulant : NiSO4 solution for control stimulation ( refrigerated, 1 vial)
Measurement reagent : ELISA kit for measurement of IL-6 produced in supernatant

MiCAN Technologies, Inc.

Evaluation and research in acommon laboratory with Kyoto University
Our headquarters and research laboratory are located in
Kyoto-University Katsura Venture Plaza, where Kyoto University
nurtures the creation of new businesses utilizing new
ideas/technologies and intellectual properties.

Kyoto-University Katsura Venture Plaza, 1-36, Goryo-ohara,
Kyoto Nishikyo-ku, Kyoto 615-8245, Japan
Kyoto-University Katsura Venture Plaza

Patent application for non-stimulated dendritic cells for research of viruses (Second product)
Adopted for the Economic Gardening Support Grant supported by Kyoto Industrial Support Organization 21
Certified as Management of Wisdom by the Kyoto Chamber of Commerce and Industry (2018)075-381-3008
http://micantechnologies.com
info@micantechnologies.com