̐̎̐̎೧݆̑̐̔ೖ ৿ ‫ ׃‬୉ ָ৪෼͹ਫ਼ࢊ੓޴৏ͶࣁͤΖޭιϱγʖ͹‫׈‬੓੏‫ߑؽޜ‬Νմ໎৿‫׃‬୉ָࣙષՌָ‫ܧ‬ʤཀྵָ෨ʥ͹ठҬୣ໷‫گ‬दΔ͹‫ڂݜ‬ήϩʖϕͺɼ৪෼͗Ή΁͢͏ޭͶ΍ɼ͖͖ͤ͵ޭͶ΍ࣰ֮ͶԢ౶ͤΖޭ‫״‬ण੓͹߁͗͠ɼ৪෼ޭιϱγʖνϱϏέ࣯ʰϓΧφφϫϒϱʱ͹‫׈‬੓੏‫ޜ‬ͶΓͮͱࣰ‫͠ݳ‬ΗΖ͞ͳΝ൅‫͢ݡ‬Ήͪ͢ɽຌ‫ڂݜ‬͹੔Վ͗൅ఴͤΖ͞ͳͶΓΕɼ৪෼͗‫ڧ؂‬ͶనԢ͢͵͗Δ੔ௗͤΖ࢕ૌΊ͹ཀྵմ͗਄ΉΖͫ͜Ͳ͵͚ɼ‫ڧ؂‬నԢ੓͗ྒྷ͚ɼफྖ͹߶͏ࡠ෼͹૓ड़Ͷ΍‫͗ܪ‬Ζ͞ͳ͗‫غ‬ଶ͠ΗΉͤɽຌ‫ڂݜ‬੔Վͺɼ2020 ೧ 3 ݆ 26 ೖʤೖຌ࣎ؔʥ෉͜Ͳธࠅ৪෼ਫ਼෼ָճࢿʲThe Plant CellʳΨϱϧ΢ϱ൝Ͷ‫͠ࡎܟ‬ΗΉͪ͢ɽʴຌ‫ڂݜ‬੔Վ͹ϛ΢ϱφʵz ৪෼͹ޭ۸੓ ஭  Ͷಉ͚ޭιϱγʖʰϓΧφφϫϒϱʱ஭  ͹‫׈‬੓Ν 537 νϱϏέ࣯͗ཊ੏ͤΖ͞ͳΝ൅‫ݡ‬z ϓΧφφϫϒϱͺ 537 νϱϏέ࣯ྖΝ௒ઇͤΖ͞ͳͲɼࣙਐ͹‫׈‬੓য়ସΝ࠹నԿͤΖ͞ͳΝ൅‫ݡ‬z ຌ‫ڂݜ‬੔Վ͗൅ఴͤΖ͞ͳͲɼ৪෼͗ʹ͹Γ͑Ͷޭ‫ڧ؂‬ͶనԢ͢ͱ੔ௗͤΖ͹͖ཀྵմͲ͘Ζͫ͜Ͳ͵͚ɼਫ਼ࢊ੓͹߶͏ࡠ෼͹૓ड़΃‫͗ܪ‬Ζ͞ͳ΍‫غ‬ଶ͠ΗΉͤᶙɿ‫ڂݜ‬͹ഐ‫ܢ‬৪෼͗ɼ‫ܬ‬Ώլਫ਼͓͹ᡤ࣢ͳ͏ͮͪ‫ׯح‬Νޭ‫ݱ‬Ͷ޴͖ͮͱ੔ௗͦ͠Ζޭ۸੓ൕԢͺɼଢཇޭ͹‫Ͳޭ͏ک‬΍ɼͨ͹ 1 Ա෾͹ 1 ͹ඏओ͵ޭͲ΍Ӏً͘͞͠ΗΖൕԢͲ͍Εɼ‫݊ئ‬੓͹͍ΖൕԢͲͤɽ૔ࢢཁ৪෼εϫ΢ϊψθψ ஭ 3 ͹ޭιϱγʖʀϓΧφφϫϒϱ 1 (phot1) ͺɼඏओޭ͖Δ‫ޭک‬ΉͲɼޭ‫ ౕ͗ک‬8 ܽҐ৏΍ҡ͵Ζ੪৯ޭΝ‫״‬எ͢ͱޭ۸੓Ν༢಍ͤΖ͞ͳ͗Ͳ͘Ήͤɽʹ͹Γ͑Ͷ͢ͱୱҲ͹ޭιϱγʖ͗͞ΗΆʹ߁͏ξ΢ψϝρέϪϱζΝࣰ‫ͱ͢ݳ‬͏Ζ͹͖ɼͨ͹࢕ૌΊͺΓ͚෾͖ͮͱ͏ΉͦΞɽըʓͺ͞ΗΉͲɼRPT2 ͳ‫ͻݼ‬ΗΖҾࢢ͗ՁΔ͖͹ࡠ༽ͶΓͮͱ phot1 ͹ޭ‫״‬ण੓ΝԾ͝ɼ‫ޭک‬ͶనԢͤΖ࢕ૌΊΝಉ͖ͦΖ͞ͳΝ໎Δ͖Ͷ͢ͱ͏Ήͪ͢ (Sakai et al., 2000, Haga et al., 2015)ɽ
ᶚɿ‫ڂݜ‬͹֕གྷຌ‫Ͳڂݜ‬ͺɼphot1 ͗ޭΝ‫״‬எͤΖͳͨ͹߮ો‫׈‬੓͗৏তͤΖ͞ͳͶ஥‫͢ء‬ɼRPT2 ͗phot1 ͹ޭͶԢ౶ͪ͢‫׈‬੓ԿΝཊ੏ͤΖͳ͏͑ՀઈΝཱིͱΉͪ͢ɽͨ͞ͲɼRPT2 νϱϏέ࣯͹ਫ਼ࢊྖ͗ਜ਼৙͵εϫ΢ϊψθψ໼ਫ਼‫ה‬ɼRPT2 ΝࣨͮͪಧષรҡରɼRPT2 Νգ৔Ͷਫ਼ࢊͤΖ‫ה‬ɼͨΗͩΗͶޭΝ౲ͱ phot1 ͹‫׈‬੓Νർֳ͢Ήͪ͢ɽͤΖͳɼ໼ਫ਼‫ה‬͹ phot1 Ͷർ΄ͱ rpt2 ͗ܿࣨͪ͢ಧષรҡର͹ phot1 ͺ‫׈‬੓͗߶͚ɼգ৔ਫ਼ࢊ‫ה‬͹ phot1 ͺ‫׈‬੓͗ఁ͚͵ͮͱ͏Ήͪ͢ɽ͞͹ͳ͘ɼRPT2 ͺ phot1 ͹‫׈‬੓௒ઇ෨ҒͲ͍Ζ LOV1 ͳ໌෉͜Δ
Ηͪχϟ΢ϱͶ݃߻͢ͱ͏Ζ͞ͳΝ໎Δ͖Ͷ͢Ήͪ͢ɽ͞ΗΔ͹݃Վ͖ΔɼRPT2 ͺ phot1͹‫׈‬੓ԿΝཊ੏ͤΖ௒ઇҾࢢͲ͍Ζ͞ͳ͗໎Δ͖Ͷ͵ΕΉͪ͢ɽ  Ήͪɼຌ‫Ͳڂݜ‬ͺɼRPT2 νϱϏέ࣯͗ҋॶͶ͕͏ͱϤϑΫοϱʷϕϫτΠλʖϞνϱϏέ࣯෾մ‫ ߑؽ‬஭ ̒ ͶΓͮͱ෾մ͠ΗΖ͞ͳɼphot1 ͗ RPT2 ͹෾մΝཊ੏ͤΖ͞ͳ΍൅‫͢ݡ‬Ή ͢ ͪ ɽ ‫ ع‬Ͷ ໎ Δ ͖ Ͷ ͵ ͮ ͱ ͏ ͪ RPT2 Ҫ ఽ ࢢ ఺ ࣺ ੏ ‫ ޜ‬஭ 5 ͳ ߻ Κ ͦ Ζ ͳ (TsuchidaMayama et al., 2010)ɼRPT2 νϱϏέ࣯͹ਫ਼ࢊྖ͗ҋॶΏओޭԾͲͺఁ͚ɼ‫ޭک‬ԾͲͺ߶͚͵ΖΓ͑ξ΢ψϝρέͶ௒ઇ͠ΗΖ͞ͳͲɼRPT2 ͗͏ΚͻՆรఏ߇‫ؽͱ͢ͳح‬೵͢ɼҋॶΏඏओޭԾͲͺ phot1 ͗‫׈‬੓Կ͢Ώ͚ͤɼ‫ٱ‬Ͷ‫ޭک‬ԾͲͺ phot1 ͺգ৔Ͷ‫׈‬੓Կ͢͵͚͵
Ζͳߡ͓ΔΉͪ͢ɽᶛɿࠕ‫ޛ‬͹ఴ֋ຌ‫ڂݜ‬ͶΓͮͱɼ৪෼͗ෆ‫݊ئ͖ͯ״‬Ͷޭ۸੓Νࣖͤ೵ྙ͹‫خ‬൭ͶɼୱҲ͹ޭιϱγʖ͹‫׈‬੓Ν෾ࢢՆรఏ߇‫ح‬ͶΓͮͱઊຽͶ੏‫ͤޜ‬Ζ࢕ૌΊ͍͗Ζ͞ͳ͗໎Δ͖Ͷ͵ΕΉͪ͢ɽ
phot1 ͺޭιϱγʖͳ͢ͱޭ۸੓͹Ί͵Δͥɼޭ߻੔‫׈‬੓௒ઇͶಉ͚‫޳֋޺ـ‬ӣಊɼཁྚରޭఈҒӣಊͶ‫ؖ‬ΚΕɼΉͪԻౕιϱγʖͳ͢ͱ৪෼͹‫ـ‬ԻనԢͶ΍ಉ͚͞ͳ͗࠹ۛ໎Δ͖Ͷ͵Ε͍ͯͯΕΉͤɽΉͪޭͶΓͮͱߑଆรԿͤΖ෾ࢢυώ΢ηɼLOV νϱϏέ࣯ͳ͢ͱ೶Ώࡋ๖͹ޭҪఽָద‫ڂݜ‬ςʖϩͳ͢ͱཤ༽͠Ηͱ͏Ήͤɽຌ‫ڂݜ‬੔Վ͗൅ఴͤΖ͞ͳͶΓΕɼޭ߻੔೵Ν߶Όͪफྖ͹߶͏ࡠ෼͹૓ड़Ώɼ৪෼ՌָΝ௔͓ͱ߁͚ਫ਼෼Ռָ෾໼Ͳཤ༽͠ΗΖ෾ࢢυώ΢ηςʖϩ͹֋൅Ͷ‫͗ܪ‬Ζ͞ͳ͗‫غ‬ଶ͠ΗΉͤɽᶜɿ‫ڂݜ‬੔Վ͹ޮන͞ΗΔ͹‫ڂݜ‬੔Վͺ 2020 ೧ 3 ݆ 26 ೖʤೖຌ࣎ؔʥ෉͜Ͳɼธࠅ৪෼ਫ਼෼ָճࢿʲThePlant Cellʳ͹Ψϱϧ΢ϱ൝Ͷ‫͠ࡎܟ‬ΗΉͪ͢ɽ࿨ ช ν ΢ φ ϩ : Arabidopsis ROOT PHOTOTROPISM2 is a Light-Dependent Dynamic
Modulator of Phototropin1.
ஸ ं : Taro Kimura, Tomoko Tsuchida-Mayama, Hirotatsu Imai, Koji Okajima, Kosuke Ito,Tatsuya Sakai
DOI: https://doi.org/10.1105/tpc.19.00926
9ɿ‫ࢩڂݜ‬ԋຌ‫ڂݜ‬ͺೖຌָढ़৾‫ڷ‬ճ͹Ռָ‫ڂݜ‬ඇঁ੔ࣆ‫ۂ‬ʤ22570058ɼ25120710ɼ16H01231ɼ
17H03694ɼ18K19329ʥͶΓΖࢩԋ͹ԾɼߨΚΗΉͪ͢ɽຌ݇Ͷ‫ͤؖ‬Ζ͕໲͏߻Κͦ઎৿‫׃‬୉ָࣙષՌָ‫ܧ‬ʤཀྵָ෨ʥ‫گ‬द ठҬ ୣ໷ʤ͖͠͏ ͪͯΏʥ(PDLOʁWVDNDL#JVQLLJDWDXDFMSʽ༽‫ޢ‬մઈʾ஭ 1ޭ۸੓‫ܬ‬Ώլਫ਼͓͹ᡤ࣢͹੔ௗ๏޴Νޭ‫ݱ‬๏޴Ͷ޴͜Ζ͞ͳͶΓͮͱɼΓΕޭΝ‫ٷ‬फ͢ޭ߻੔‫׈‬੓Ν߶ΌΖͪΌͶಉ͚৪෼͹ޭ‫ڧ؂‬Ԣ౶ɽ19 ੊‫ن‬ͶξʖΤΡϱͶΓͮͱ؏ࡱʀ‫ى‬फ़͠Ηɼ20 ੊‫ن‬Ͷ͕͜Ζ৪෼ϙϩϠϱʀΨʖΫεϱ͹൅‫ݡ‬Ͷͯ͵͗ͮͪɽޭ۸੓Ͳͺɼޭ͹౲ͪͮͪ‫ׯح‬͹ޭলࣻଈͳӆଈͳ͹ؔͶΨʖΫεϱೳౕ‫ޱ‬ഓ͗ਫ਼ͣɼ͞Η͗‫಼ׯح‬Ͷ෈‫ۋ‬౵͵੔ௗΝ΍ͪΔͤͪΌͶ۸‫ۄ‬੔ௗً͗͞Ζͳߡ͓ΔΗͱ͏Ζ͗ɼາͫͶͨ͹ϟΩωθϞͶͺ෈໎͵఼͗ଡ͏ɽ
஭ 2ϓΧφφϫϒϱྚ૸ྪ͖Δඅࢢ৪෼ΉͲ߁͚ฯଚ͠Ηͪ৪෼ͶಝҡదͶଚࡑͤΖ੪৯ޭण༲ରͲɼޭ۸੓ɼཁྚରޭఈҒӣಊɼ‫޳֋޺ـ‬ӣಊ͵ʹ͹৪෼͹ޭ‫ڧ؂‬Ԣ౶Ͷಉ͚ɽ2 ͯ͹ LOV χϟ΢ϱͶ 1ͯ͹νϱϏέ࣯Ϩϱ࢐Կ߮ોχϟ΢ϱΝ΍ͬɼ2 ͯ͹ LOV χϟ΢ϱͶͺͨΗͩΗ੪৯ޭΝ‫ٷ‬फͤΖϓϧϑϱϠόϊέϪΨοχ͗݃߻͢ͱ͏ΖɽLOV χϟ΢ϱͶ͕͜Ζ੪৯ޭ‫ٷ‬फ͗ϓΧφφϫϒϱޭण༲ର͹ߑଆΝรԿͦ͠ɼޭ৚ๅ͹ड़ྙΝ୴͑νϱϏέ࣯Ϩϱ࢐Կ߮ોχϟ΢ϱ͗‫׈‬੓Կ͢ɼࡋ๖಼ͲҲ࿊͹εήψϩఽୣ͕͗͘Ζͳߡ͓ΔΗͱ͏ΖɽΉͪ͞͹ߑଆรԿ͹ଐ͠ͺԻౕͶΓͮͱรԿͤΖͪΌɼϓΧφφϫϒϱ͗Իౕιϱγʖͳ͢ͱ΍‫ؽ‬೵ͤΖ͞ͳ͗ๅࠄ͠Ηͱ͏Ζɽ஭ 3εϫ΢ϊψθψ

ָ໌ͺ Arabidopsis thalianaɽ ΠϔϧψՌεϫ΢ϊψθψ଒͹ 1 ೧૴ɽ2000 ೧Ͷ৪෼ͲॵΌͱΰόϞԚ‫خ‬ഓ྽͗‫׮‬સͶմಣ͠ΗΖ͵ʹɼ৪෼͹ਫ਼ໍ‫ݳ‬েΝཀྵմͤΖͪΌͶ࠹΍ྒྷ͚‫ڂݜ‬͠Ηͱ͏ΖϠυϩࣰ‫ݩ‬৪෼ɽ
஭ 4ϤϑΫοϱʷϕϫτΠλʖϞνϱϏέ࣯෾մ‫ߑؽ‬ਇ֫ਫ਼෼Ͷฯଚ͠ΗͪνϱϏέ࣯෾մ‫͍Ͳߑؽ‬Εɼ༹ʓ͵ਫ਼ໍ‫ݳ‬েͶ‫ؖ‬༫ͤΖɽ෾մ͹ඬదͳ͵ΖνϱϏέ࣯ͶͺɼϤϑΫοϱ νϱϏέ࣯͗෵਼‫ݺ‬࿊͵ͮͪ࠱͗෉Յ͠ΗΖϛϨϤϑΫοϱԿً͗͞ΖɽϛϨϤϑΫοϱԿඬࣟ͠ΗͪνϱϏέ࣯ͺϕϫτΠλʖϞͳ‫ͻݼ‬ΗΖνϱϏέ࣯෵߻ର಼Ͷ͕͏ͱ෾մ͠ΗΖɽ஭ 5఺ࣺ੏‫ޜ‬νϱϏέ࣯͹Πϝό࢐ഓ྽͗‫ى‬࿧͠ΗΖҪఽࢢ͹ DNA Ԛ‫خ‬ഓ྽Νல‫ܗ‬ͶɼRNA ͗߻੔͠ΗΖգఖΝ఺ࣺͳ‫΁ݼ‬ɽ఺ࣺ͠Ηͪ RNA Ԛ‫خ‬ഓ྽Ν‫ݫ‬ͶνϱϏέ࣯͗߻੔͠ΗΖɽͨ͹ͪΌɼҪఽࢢ͹఺ࣺΝཊ੏͍Ζ͏ͺଇ਒ͤΖΓ͑Ͷ੏‫ͤޜ‬Ζ͞ͳͲɼͨ͹‫ޛ‬Ͷ߻੔͠ΗΖνϱϏέ࣯ྖΝรԿͦ͠Ζ͞ͳ͗Ͳ͘Ζɽʽࢂߡࣁྋʾ

ਦ 1. εϫ΢ϊψθψԭԿլਫ਼͓ᡤ࣢͹ޭ۸੓ԥ͖Δ੪৯ޭΝলࣻͤΖͳɼ͕Γ್ͨ࣎ؔ‫ޛ‬Ͷͺլਫ਼͓͹ᡤ࣢ͺޭ‫ݱ‬๏޴΃ͳ۸‫ͤۄ‬ΖɽҋॶͲүͱͪԭԿլਫ਼͓͵͹Ͳɼࢢཁͺ֋͏ͱ͕Δͥϓρέ͗‫ܙ‬੔͠Ηͱ͏Ζɽࣺਇͺࢢཁਜ਼໚͖Δ͹ԭԿլਫ਼͓ɽࠪͺޭলࣻ઴ɼӊͺ੪৯ޭলࣻ‫ޛ‬ɼ3 ࣎ؔ‫ޛ‬͹༹ࢢʤࣺਇࡳӪʀ๗ծ݊ʥɽ

ਦ 2. RPT2 ෾ࢢՆรఏ߇‫ح‬ͶΓΖ phot1 ‫׈‬੓௒ઇ‫ߑؽ‬͹Ϡυϩਦ
ओޭԾʁRPT2 νϱϏέ࣯͹ਫ਼ࢊྖ͗ఁ͚ɼphot1 ͗‫׈‬੓Կ͢Ώͤ͏ɽphot1 ͺΚ͖ͥ͵ޭ͹೘ྙ͖Δ࠹୉‫ݸ‬͹εήψϩΝड़ྙͲ͘ΖͪΌɼඏओ͵࢙ܻͲޭ۸੓Ν͕ͤ͞͞ͳ͗Ն೵Ͷ͵Ζɽ‫ޭک‬Ծʁޭ͗ RPT2 νϱϏέ࣯͹ਫ਼ࢊྖΝ৏তͦ͠ɼphot1 ͗‫׈‬੓Կ͢ͰΔ͚͵Ζɽޭ͗୉ྖͶ೘ྙ͢ͱ΍ phot1 ͹‫׈‬੓ԿͺనౕͶཊ੏͠Ηͱ๠࿪ͦͥɼޭলࣻଈͳӪଈ͹ޭ͹‫͠ک‬Ν‫ݡ‬෾͜ɼޭ۸੓Ν͕ͤ͞͞ͳ͗Ն೵Ͷ͵Ζɽ