Histone Deacetylase Phylogeny

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Class I & II HDAC Enzyme Phylogeny
constructing phylogenetic relationships of HDACs from molecular sequences in vertebrates

Here are shown phylogenies of the two classical HDAC classes across vertebrate species. The classical HDAC
enzymes fall into two phylogenetic classes, Class I and II. The Class I enzymes are HDAC1, 2, 3 & 8 and are typicallyfound in the nucleus where they can repress transcription. The Class II enzymes, HDAC4, 5, 6, 7, 9 & 10, are able tomove between the cytoplasm and nucleus to function in signal transduction.
Class I HDAC enzyme phylogeny
Q9BY41 (Human)
NP_001069699.1 (Cow)
XP_001510728.1 (Platypus)
XP_538078.2 (Dog)

HDAC8

XP_001093861.1 (Rhesus monkey)
NP_081658.1 (Mouse)
XP_343805.2 (Rat)
XP_001140206.1 (Chimpanzee)
XP_001488396.1 (Horse)
CAH90984.1 (Orangutan)
O15379 (Human)
XP_001093955.1 (Rhesus monkey)
NP_034541.2 (Mouse)
NP_445900.1 (Rat)
XP_001504028.1 (Horse)
XP_001368824.1 (Opossum)

HDAC3

NP_001016883.1 (Frog)
NP_990078.1 (Chicken)
XP_001150836.1 (Chimpanzee)
NP_957284.1 (Zebrafish)
NP_001005432.1 (Frog)
NP_001068614.1 (Cow)

HDAC2

NP_990162.1 (Chicken)
Q92769 (Human)
XP_518700.2 (Chimpanzee)
NP_032255.2 (Mouse)
XP_342150.2 (Rat)
XP_001368989.1 (Opossum)
XP_532270.2 (Dog)
NP_775343.1 (Zebrafish)
NP_001081491.1 (Frog)

HDAC1

NP_989487.1 (Chicken)
XP_001163573.1 (Chimpanzee)
XP_001499986.1 (Horse)
Q13547 HDAC1 (Human)
NP_004955.2 (Human)
NP_001020580.1 (Rat)
NP_032254.1 (Mouse)
XP_544435.2 (Dog)
NP_001032521.1 (Cow)

North America 877 222 9543 Europe +32 (0)2 653 0001 Japan +81 (0)3 5225 3638

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Class II HDAC enzyme phylogeny

HDAC6
NP_001092430.1 (Cow)

XP_855362.1 (Dog)
XP_001493915.1 (Horse)
XP_001100149.1 (Rhesus monkey)
Q9UBN7 (Human)
XP_615498.3 (Cow)

NP_001080486.1 (Frog)Q9YGY4 (Frog)
Q99N13 (Mouse)
Q5ZKH6 (Chicken)
XP_001104208.1 (Rhesus monkey)
Q9UKV0 (Human)
XP_001150521.1 (Chimpanzee)
XP_228753.4 (Rat)
NP_034543.2 (Mouse)
XP_543715.2 (Dog)
XP_522368.2 (Chimpanzee)
Q8WUI4 (Human)
XP_001490085.1 (Horse)
XP_345869.2 (Rat)
NP_062518.2 (Mouse)
XP_001371963.1 (Opossum)
XP_849514.1 (Dog)
XP_595731.3 (Cow)

HDAC9
HDAC7

HDAC4
NP_989644.1 (Chicken)
XP_001099472.1 (Rhesus monkey)
XP_001364322.1 (Opossum)
XP_001073452.1 (Rat)

HDAC10

XP_343630.3 (Rat)
NP_997108.1 (Mouse)
P56524 (Human)
XP_001497201.1 (Horse)

XP_848669.1 (Dog)
NP_001068928.1 (Cow)
XP_001112171.1 (Rhesus monkey)
Q96958 (Human)

NP_954668.1 (Mouse)
XP_001339504.1 (Zebrafish)
XP_001081495.1 (Rat)
Q9Z2V6.2 (Mouse)
NP_034542.3 (Mouse)
AAN46420.1 (Chinese hamster)
XP_548064.2 (Dog)
XP_001490684.1 (Horse)
Q9UQL6 (Human)
CAH91758.1 (Orangutan)
XP_511542.2 (Chimpanzee)
XP_001100255.1 (Rhesus monkey)

HDAC5
Active Motif’s HDAC Assay Kits are easy,
sensitive assays to determine the activityof Class I and II HDAC enzymes. Please visitwww.activemotif.com for more information
about our kits and related HDAC antibodies.

METHODS

Sequence alignment and phylogenetic tree generation
were created using Geneious Pro software fromBiomatters.

ProductFormat

Catalog No.

HDAC Assay Kit (Fluorescent)1 x 96 rxns

56200

HDAC Assay Kit (Colorimetric)

1 x 96 rxns

56210

North America 877 222 9543 Europe +32 (0)2 653 0001 Japan +81 (0)3 5225 3638