The Histone CodeDomain
14-3-3Modification
H3 Ser10 Phos, Ser28 Phos

Proteins
14-3-3 Family

Ank

H3 Lys9 Methyl

GLPBIR

H3 Thr3 Phos

Survivin

BRCT

H2AX Ser139 Phos

53BP1, BRCA1, MDC1,NBS1Bromo

H3 Lys9 Acetyl
H3 Lys14 Acetyl
H4 Lys5 Acetyl
H4 Lys12 Acetyl

BRD4, BAZ1B
BRD4, BAZ1B, BRG1
BRD4BRD2, BRD4Chromo

H3 Lys4 Methyl
H3 Lys9 Methyl
H3 Lys27 Methyl
H3 Lys36 Methyl

CHD1
CDY, HP1, MPP8,CDY, Pc
MRG15MBT

H3 Lys9 Methyl
H4 Lys20 Methyl

L3MBTL1, L3MBTL2L3MBTL1, MBTD1PID

H2AX Tyr142 Phos

APBB1PHD

H3 Lys4 Methyl
H3 Lys9 Methyl
H4 Lys20 Methyl

BPTF, ING, RAG2,BHC80, DNMT3L,PYGO1, JMJD2A
UHRF
JMJD2A, PHF20

TDR

H3 Lys9 Methyl
H3 Arg17 Methyl
H4 Lys20 Methyl

TDRD7
TDRD353BP1

WD

H3 Lys4 Methyl
H3 Lys9 Methyl

WDR5EED* Strahl BD, Allis CD (2000). The language of covalent
histone modifications. Nature 403: 41-45.
** Image courtesy of Dr. Ray Trievel,
Department of Biological Chemistry,
University of Michigan.

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The “histone code” hypothesis put
forward in 2000* suggests that specifichistone modifications or combinationsof modifications confer unique biological
functions to the regions of the genome
associated with them, and that specialized binding proteins (readers) facilitate
the specialized function conferred by thehistone modification. Evidence is accumulating that these histone modification /binding protein interactions give rise todownstream protein recruitment, potentially facilitating enzyme and substrateinteractions or the formation of uniquechromatin domains. Specific histone
modification-binding domains have been
identified, including the Tudor, Chromo,Bromo, MBT, BRCT and PHD motifs.

FIGURE
Resetting Histone Methylation. Crystal structure**of the catalytic domain of the histone demethylase JMJD2A bound to a peptide derived from theamino terminus of histone H3, trimethylated atlysine 9 (yellow carbon atoms). Also shown is thesubstrate alpha-ketoglutarate (green) and a Ni(II)cation (red) that is substituted for the Fe(II)normally present.

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AcAcetylation

MeMethylation

UUbiquitylation

CitCitrullinationwww.activemotif.com

P

Phosphorylation
StructuredDomain

Histone Variants
HistoneVariant

Identity vs.
Core Histone

H2AX96%

DNA damage repair; Ser in the SQEY
motif in mammalian H2AX isphosphorylated by ATM and ATR in
response to double strand DNA breaks

H2A.Z

60-65%

Transcriptional regulation,
DNA damage, chromosome stability

macro
H2A

64%

Enriched on inactive mammalian
X chromosome; uniqueN-terminal addition

CENP-A

Highly diverged

Centromere-specific;
specialized H3 variant forattachment of kinetochore

H3.3

97%

Functions

In addition to the core histones (H2A,H2B, H3 and H4), there are specializedversions (histone variants) of the histone
proteins that exhibit unique distribution
patterns and have roles not served bytheir more common paralogs. For
example, the histone H2A variant, H2AX,
is phosphorylated at a unique site in its
C-terminus by the ATM protein in
response to double-strand DNA breaks.
Phospho-H2AX takes part in the mechanism of double strand break repair.
See the table to the left for a summaryof these variants and their functions.

Replaces H3.1 at transcriptionallyactive regions; varies at 5 amino acids

CENP-A
Unique N-terminus of the histone H3 variant, CENP-A.

H2A.X
Unique C-terminus of the histone H2A variant, H2AX.

H3.3

Regions of sequence divergence (residues in YELLOW ) of the histone H3 variant, H3.3.

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Histone Modifications and Animal Models
AcetylMonomethyl
H3
Lysine 4 Dimethyl
Trimethyl
AcetylMonomethyl
H3
Lysine 9 Dimethyl
Trimethyl
AcetylMonomethyl
H3
Lysine 27 Dimethyl
Trimethyl
AcetylMonomethyl
H3
Lysine 36 Dimethyl
TrimethylAcetyl
Monomethyl
H3
Lysine 56 Dimethyl
TrimethylAcetyl
Monomethyl
H3
Lysine 79 Dimethyl
Trimethyl

Tet
+/–
+++
++
++
+++
+/– (?)
–
++
+++
+++
++
+
+/–
++
–
++
+++
+
–
–

Yeast
–
+++
++
++
+
–
–
–
+++
–
–
–+/–
++
++
++
+++
–
–
–

Fly
n/d
+++
++
+
n/d++
+
++
n/d+
+++
+++
n/d++
++
+/–
n/d
n/dn/dn/d

–
+
–
–

–
++
++
++

n/dn/d++–Mouse Human
+/–
+
++
++
+
++/–
+/–
+/–
+/–
++
++
++
++
+++
+++
+
+/–
++
+++
+++
+++
++
++
–+/–
++
++
++
++
+/–
+/––+/––+–––+++–++–+/–++–

While the histone proteins are very
highly conserved from lower eukaryotes (such as budding yeast) up tohumans, many of the histone modifications are not found in all species.
Budding yeast, for example, do notcompact their genome to the same
degree as metazoans, and therefore
do not require the enzymes that deposit many of the methylation marks
associated with heterochromatin.
TA B L E
Comparison of acetylation and methylation on specific lysine residues onhistone H3 between Tetrahymena (Tet),
Saccharomyces (Yeast), Drosophila (Fly),
Mouse and Human.
Mouse and Human Modifications associated with transcriptional activation arecolored in PURPLE.
Modifications associated with transcriptional repression are colored in TAN.
n/d = Not determined.

Immunodetection of Histone Acetylation in HeLa Cells.
Red: alpha Tubulin mAb (Cat. No. 39527)
Green: Histone H3 acetyl Lys23 pAb (Cat. No. 39131)
Blue: DAPI
Right: Merge of all 3 images.

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Histone Modifications & Associated Functions
Residue

Modification

FunctionsWriterEraser

ReaderPhosphorylation
Acetylation
Ubiquitylation
Phosphorylation

MitosisTranscriptional activation
Spermatogenesis
Mitosis

PKC
CBP, p300, HAT1
RNF1
NHK1

Ser139Phosphorylation

Tyr142

Phosphorylation

DNA Damage repair,
apoptosis
Regulation of DNA
damage foci formation

ATM, ATR

PP4

BAZ1B

EYA

MDC1BRCT, NBS1BRCT,
53BP1BRCT, TDRr, BRCA1BRCTAPBB1PID

Histone H2A
Ser1
Lys5
Lys119Thr120

Histone H2AX

Histone H2B
Lys5
Lys12
Ser14
Lys15
Lys20
Lys120

Acetylation
Acetylation
Phosphorylation
Acetylation
Acetylation
Ubiquitylation

Transcriptional activation p300
Transcriptional activation CBP, p300
Apoptosis
MST1
Transcriptional activation CBP, p300
Transcriptional activation CBP, p300
Transcription (elongation?) RNF20

Arg2
Thr3
Lys4
Lys4

Methylation
Phosphorylation
Acetylation
Methylation

Transcriptional activation
MitosisTranscriptional activation
Euchromatin, transcriptional activation

CARM1, PRMT6
Haspin, Vrk1

JMJD6

MLL, SET1, ASH1,
SET7, SMYD2LSD1/BHC110,
JHDM1A/ KDM2A,
JHDM1B/KDM2B,
JARID1A/KDM5A,
JARID1B/ KDM5B,
JARID1C/KDM5C,JARID1D/KDM5D

Thr6
Arg8
Lys9

Phosphorylation
Methylation
Acetylation

PKCb
PRMT5
GCN5, PCAF, SRC1 SIRT6

Lys9

Methylation

Transcriptional activation
Transcriptional activation
Histone deposition,
Transcriptional activation
Transcriptional silencing,heterochromatin

Ser10

Phosphorylation

Histone H3

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Mitosis, immediate earlygene activation

SurvivinBIR

SUV39H1/2, G9a, JMJD1A/KDM3A,
CLL8, SETDB1,
JMJD1B/ KDM3B,
EuHMT1, Riz1
JMJD1C/TRIP8,
JMJD2A/KDM4A,
JMJD2B/ KDM4B,
JMJD2C/KDM4C,
JMJD2D/KDM4D
Aurora B, MSK1, PP1IKKa

CHD1CHR, ING FamilyPHD,RAG2PHD, TAF3PHD,
BPTFPHD, BRD, BHC80PHD,
DNMT3LPHD, PYGO1PHD,
JMJD2APHD, TDR, WDR5WD

BRD4BRD, BAZ1BPHD, BRD
L3MBTL1/L2MBT, HP1CHR,
MPP8CHR, ANK, CDY FamilyCHR,
TDRD7TDR, UHRFPHD, EEDWD,GLPANK

14-3-314

Histone Modifications & Associated Functions (cont.)
Residue

Modification Functions

WriterEraserReaderHistone H3, cont.
Thr11

Lys23
Arg26
Lys27

Phosphorylation Mitosis; DNA damage
Dlk
induced transcription
Acetylation
Transcriptional activation GCN5, PCAF, CBP,
p300, MOZ, MORF,
TIP60, SRC1
Methylation
Transcriptional activation CARM1
Acetylation
Transcriptional activation GCN5, PCAF, CBP,
p300
Acetylation
Transcriptional activation GCN5, PCAF, p300
Methylation
Transcriptional activation CARM1
Methylation
Transcriptional silencing EZH2, EZH1

Ser28
Lys36
Lys36

Phosphorylation Mitosis
Acetylation
Transcription activation
Methylation
Transcription elongation

Thr45

Phosphorylation DNA replication,
PKCd
apoptosis
Acetylation
DNA damage repair,
CBP, p300
chromatin assembly
Methylation
Transcriptional activation DOT1L

Lys14
Arg17
Lys18

Lys56
Lys79

Aurora B
GCN5, PCAF
NSD1, SET2, SMYD2,NSD2

BRD4BRD, BAZ1BPHD, BRD,
BRG1BRD
TDRD3TDRJMJD1A/ KDM3A, JMJD1B/KD- PcCHR,
M3B, KDM6A/UTX, JMJD3/ CDY FamilyCHR
KDM6B
14-3-314
JHDM1A/KDM2A, JHDM1B/
KDM2B, JHDM3A/KDM4A,
JHDM3B/KDM4B, JHDM3C/
KDM4C, JHDM3D/KDM4D

MRG15CHR

JMJD6

TDRD3TDRBRD4BRDHistone H4
Ser1
Arg3
Lys5
Lys8
Lys12
Lys16
Lys20

Lys91

Phosphorylation Transcriptional activation CKII
Methylation
Transcriptional activation PRMT1, PRMT5Acetylation
Histone deposition,
ATF2, HAT1, CBP,
transcriptional activation p300, TIP60, HBO1
Acetylation
Transcriptional activation CBP, p300, TIP60,
HBO1
Acetylation
Histone deposition,
HAT1, CBP, p300,transcriptional activation TIP60, HBO1
Acetylation
Transcriptional activation MOF, TIP60
Methylation
Transcriptional silencing, PR-SET7 (mono),
heterochromatin
SUV420H1 (di),MMSET, SUV420H2(tri)Acetylation
Histone deposition,
HAT1DNA damage repair
Domain Key

1414-3-3 domain
ANK Ankyrin repeat domain
BIR Baculovirus IAP repeat
BRCT BRCA1 C-term domainwww.activemotif.com

BRD
CHR
MBT
PID

BRD2BRD, BRD4BRD
SIRT1Bromodomain
Chromodomain
MBT domain
Phosphotyrosine interaction domain

L3MBTL1MBT, MBTD1MBT,JMJD2APHD, PHF20PHD
53BP1BRCT, TDRPHD PHD zinc finger domain
TDR Tudor domain
WD WD40 repeat domain

Histone Modification Antibodies
Description

Reactivity Applications Format Cat. No.

Reactivity Applications Format Cat. No.

HISTONE H3

HISTONE H1
Histone H1 pAb

Description

H, WRHistone H1 pAb

DHistone H1.5 phospho Ser17 pAb

H

100 µl39707

Histone H3 mAb

H, WR

ChIP, IF, WB 100 µg

ChIP, IF, IP, WB 100 µl

39575

Histone H3, C-terminal pAb

Sc, H, WR

ChIP, WB

200 µl39163

61107

Histone H3.cs1 pAb

HWB

200 µl39573

Histone H3 acetyl pAb

H, WRChIP, WB

200 µl39139

Histone H3 dimethyl Arg2 sym. pAb

H, WRWB

100 µl

39703

Histone H3 phospho Thr3 pAb

H, WRWB

200 µl39153
39381

IF, WBWB

100 µlHISTONE H2A, H2B

39763

Histone H2A pAb

Sc, Sp

ChIP, WB

Histone H2A pAb

Sc, Sp

WB

100 µg 39945

Histone H3 acetyl Lys4 pAb

H, WR

ChIP, IF, WB

200 µlHistone H2A pAb

HWB

100 µg 39209

Histone H3 monomethyl Lys4 mAb

H, WR

ChIP, WB

100 µg 39635

Histone H2A, acidic patch pAb

H, WR

ChIP, WB

200 µl39111

Histone H3 monomethyl Lys4 pAb

H, WRIF, WB

200 µlHistone H2A, C-terminal pAb

H, WR

WB

200 µl39591

Histone H3 dimethyl Lys4 mAb

H, WR

ChIP, IF, WB 100 µg 39679

Histone H2A acetyl Lys5 pAb

H, WR

WB

200 µl39107

Histone H3 dimethyl Lys4 pAb

H, WR

ChIP, WB

H, WR

ChIP, IF, WB 100 µg

200 µl39235

200 µl39297
39141

Histone H2A acetyl Lys9 pAb

H, WR

WB

200 µl39109

Histone H3 dimethyl Lys4 pAb

Histone H2A phospho Thr120 pAb

H, WR

WB

200 µl39391

Histone H3 trimethyl Lys4 pAb

Sc, H, WR ChIP, IF, WB

200 µl39159

Histone H2A phospho Ser129 pAb

Sc

ChIP, IF, IP, WB 200 µl39271

Histone H3 trimethyl Lys4 pAb

Sc, H, WR ChIP, IF, WB 100 µg

39915

Histone macroH2A1 pAb

H, M

IF, WB

200 µl39593

Histone H3 monomethyl Arg8 pAb

H, WR

100 µl39673

Histone macroH2A1.1 pAb

HIF, IHC, WB

100 µg39871

Histone H3 dimethyl Arg8 asym. pAb

Histone macroH2A2 pAb

WB39913HWB

100 µl39651

100 µg 39873

Histone H3 acetyl Lys9 pAb

H, WR

ChIP, WB

200 µl39137

IF, WB

200 µl 39585

HIF, IHC, WB

H, WR

WB

200 µl39115

Histone H3 acetyl Lys9 pAb

H, WR

Histone H2Av pAb

DWB

100 µl39715

Histone H3 acetyl Lys9 pAb

H, WR

Histone H2AX pAb

HIF, WB

100 µl

39689

Histone H3 pan-methyl Lys9 pAb

H, WR

IF, WB

200 µl

39241
39681

Histone H2A/H4 phospho Ser1 pAb

ChIP, IF, WB 100 µg

39917

Histone H2AX phospho Ser139 pAb

H, WR

IF, WB

200 µg39117

Histone H3 monomethyl Lys9 mAb

H, WR

ChIP, WB

100 µg

Htz1 pAb

Sc

ChIP, WB

100 µl39647

Histone H3 monomethyl Lys9 pAb

H, WRIF, WB

200 µl 39249

Histone H2A.Z pAb

H, WR

ChIP, WB

200 µl39113

Histone H3 monomethyl Lys9 pAb

H, WR

WB

100 µg 39887

Pht1 / H2AZ pAb

SpWB

100 µl39640

Histone H3 dimethyl Lys9 mAb

H, WR

Histone H2A.Z pAb

H, WR

ChIP, WB

100 µg 39943

Histone H3 dimethyl Lys9 pAb

H, M, WR ChIP, IF, WB

200 µl39239

H, WR

ChIP, IF, WB200 µl39375

ChIP, IF, WB 100 µg 39683

Pht1 / H2AZ acetyl pAb

SpWB

100 µl39642

Histone H3 dimethyl Lys9 pAb

Histone H2B pAb

H, WR

WB

200 µl39125

Histone H3 dimethyl Lys9 pAb

H, WR

ChIP, IF, WB 100 µg39753

Histone H2B pAb

Sc, Sp

ChIP, WB

200 µl39237

Histone H3 trimethyl Lys9 mAb

H, WR ChIP, IF, IP, WB 100 µg

61013

Histone H2B pAb

Sc, Sp

WB

100 µg 39947

Histone H3 trimethyl Lys9 pAb

Sp, H, WR ChIP, IF, WB

200 µl39161

Histone H2B pAbHWB

100 µg39210

Histone H3 trimethyl Lys9 mAb

H, WRIF, IP, WB

200 µl

39285

Histone H2B mAb

HWB

100 µg61037

Histone H3 trimethyl Lys9 pAb

H, WR

ChIP, IF, WB 100 µg

39765

Histone H2B acetyl Lys5 pAb

H, WR

ChIP, WB

200 µl

39123

Histone H3 phospho Ser10 mAb

H, WR

IF

Histone H2B acetyl Lys12 pAb

H, WR

ChIP, WB

100 µl39669

Histone H3 phospho Ser10 pAb

H, WR

ChIP, IF, WB

200 µl39253

Histone H2B phospho Ser14 mAb

H, WR

IF, IP, WB

100 µg61011

Histone H3 phospho Ser10,28 pAb

H, WR

ChIP, IF, WB

200 µl39147

Histone H2B acetyl Lys16 pAb

H, WR

ChIP, WB

200 µl39121

Histone H3 phospho Thr11 mAb

HICC, WB

100 µg39821

Histone H2B acetyl Lys46 pAb

H, WR

ChIP, WB

200 µl39571

Histone H3 phospho Thr11 pAb

H, WR

WB

200 µl39151

Histone H2B dimethyl Lys46 pAb

H, WR

IF, WB

200 µl39567

Histone H3 acetyl Lys14 pAb

H, WR

ChIP, WB

200 µl 39599

Histone H2B acetyl Lys120 pAb

H, WR

ChIP, WB

200 µl39119

Histone H3 acetyl Lys14 pAb

H, WR

ChIP, WB

100 µg 39697

Histone H2B ubiquityl Lys120 mAb

H, WR

ChIP, WB

100 µg 39623

Histone H3 dimethyl Lys14 pAb

H, WRWB

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100 µg 39636

200 µl39349

Histone Modification Antibodies (cont.)
Description

Reactivity Applications Format Cat. No.

HISTONE H3, contHistone H3 dimethyl Arg17 asym. pAb H, WR
Histone H3 acetyl Lys18 pAb

H

WB

200 µl39273

HWB

200 µl39277

100 µl39709

Histone H3 dimethyl Lys56 pAb

H, WR

ChIP, IF, WB

100 µl39693

Histone H3 acetyl Lys64 pAb

Histone H3 acetyl Lys18 pAb

H, WR

ChIP, IF, WB

H, WR

IF, WB

Histone H3 acetyl Lys18 pAb

H, WR

Reactivity Applications Format Cat. No.

Histone H3 monomethyl Lys56 pAb
WB

Histone H3 acetyl Lys18 pAb
Histone H3 monomethyl Lys18 pAb

Description

Sc, H, WR ChIP, IF, WB

200 µl 39545

200 µl 39587

Histone H3 acetyl Lys79 pAb

H, WR

ChIP, WB

200 µl 39565

200 µl39129

Histone H3 monomethyl Lys79 pAb

H, WR

ChIP, WB

200 µl39145

ChIP, IF, WB 100 µg39755

Histone H3 monomethyl Lys79 pAb

H, WRChIP, WB

100 µg

39921

39667

Histone H3 dimethyl Lys79 pAb

H, WR

ChIP, WB

200 µl39143

100 µg 39923

HWB

100 µlHistone H3 acetyl Lys23 pAb

H, WR

ChIP, IF, WB

200 µl39131

Histone H3 dimethyl Lys79 pAb

H, WR

ChIP, WB

Histone H3 monomethyl Lys23 pAb

H, WR

WB

200 µl

39387

Histone H3 monomethyl Lys122 pAb

H, WR

WB

Histone H3 dimethyl Lys23 pAb

H

WB

100 µl39653

Histone H3 acetyl Lys27 mAb

H, WRHistone H3 acetyl Lys27 pAb

Sc, H, WR ChIP, IF, WB 200 µg39133

200 µl39367

ChIP, IF, WB 100 µg 39685

HISTONE H4Histone H3 acetyl Lys27 pAb

Sc, H, WR

200 µl39135

Histone H4 pAb

H, WRWB

Histone H3 monomethyl Lys27 mAb

H, WR ChIP, IF, IP, WB 100 µg

61015

Histone H4 pan-acetyl pAb

H, WR

ChIP, IF, WB

Histone H3 monomethyl Lys27 pAb

H, WR

IF, WB

200 µl39377

Histone H4 pan-acetyl pAb

H, WR

ChIP, IF, WB 100 µg 39925

Histone H3 monomethyl Lys27 pAb

H, WR

WB

100 µg 39889

Histone H4 tetra-acetyl pAb

Sc, H, WR

IF, WB

50 µg39177

Histone H3 dimethyl Lys27 pAb

H, WR

ChIP, IF, WB

200 µl39245

Histone H4 tetra-acetyl pAb

Sc, H, WR

ChIP, WB

50 µl39179

H, WR ELISA, ICC, IP, WB 100 µg 39967

ChIP, WB

Histone H3 dimethyl Lys27 pAb

H, WR

IF, WB

100 µg39919

Histone H4 acetyl mAb

Histone H3 di/trimethyl Lys27 mAb

H, WR

ChIP, WB

200 µl39535

Histone H4 dimethyl Arg3, asym. pAb H, WR

Histone H3 di/trimethyl Lys27 mAb

H, WR

IF, WB

200 µl39536

Histone H4 acetyl Lys5 pAb

Histone H3 trimethyl Lys27 mAb

H, WR

ChIP, IF, IP, WB 100 µg

61017

200 µl 39269
200 µl39243

IF, WB

200 µl39705

H, WR

ChIP, IF, WB

200 µl39169

Histone H4 acetyl Lys5 pAb

H, WR

ChIP, IF, WB

200 µl39583

100 µg 39699

Histone H3 trimethyl Lys27 pAb

H, WR

ChIP, IF, WB 200 µg 39155

Histone H4 acetyl Lys5 pAb

H, WR

ChIP, WB

Histone H3 trimethyl Lys27 pAb

H, WR

ChIP, WB

200 µl39156

Histone H4 acetyl Lys8 pAb

H, WRWB

Histone H3 phospho Ser28 mAb

H, M, R

IF, WB

100 µl39098

Histone H4 acetyl Lys8 pAb

HHistone H3 phospho Ser28 pAb

H, WRWB

200 µl39149

Histone H4 acetyl Lys12 pAb

Sc, H, WR ChIP, IF, WB

Histone H3.3 phospho Ser31 pAb

HWB

200 µl39637

Histone H4 acetyl Lys12 pAb

Sc, H, WR ChIP, IF, WB 100 µg 39927

200 µl39171

ChIP, DB, WB 100 µl61103

200 µl39165

Histone H3 acetyl Lys36 pAb

H, WR

ChIP, IF, WB

200 µl39379

Histone H4 acetyl Lys16 pAb

Sc, D, H, WR ChIP, WB

200 µlHistone H3 dimethyl Lys36 mAb

H, WR

ChIP, IF, IP, WB 100 µg

61019

Histone H4 acetyl Lys16 pAb

Sc, D, H, WR ChIP, WB

100 µg 39929

Histone H3 dimethyl Lys36 pAb

Sc, H, WR ChIP, IF, WB

200 µl39255

Histone H4 monomethyl Lys20 mAb

H, WR

IF, WB

100 µg39727

Histone H3 dimethyl Lys36 pAb

Sc, H, WR

100 µg39891

Histone H4 monomethyl Lys20 pAb

H, WR

ChIP, IF, WB

200 µl39175

Histone H3 trimethyl Lys36 mAb

H, WR ChIP, IF, IP, WB 100 µg

61021

Histone H4 dimethyl Lys20 mAb

H, WRWB

200 µl39539

WB39167

Histone H3 trimethyl Lys36 pAb

H61101

Histone H4 dimethyl Lys20 pAb

H, WR

ChIP, IF, WB

200 µl39173

Histone H3 phospho Thr45 pAb

Sc, H, WR

WB

100 µl

39737

Histone H4 trimethyl Lys20 mAb

H, M, WR

ChIP, WB

100 µg39671

Histone H3 acetyl Lys56 mAb

Sc, Sp, H

ChIP, DB

100 µg61061

Histone H4 trimethyl Lys20 pAb

H, WR

ChIP, IF, WB

200 µl39180

Histone H3 acetyl Lys56 pAb

Sc, H

ChIP, WB

200 µl39281

Histone H4 monomethyl Lys31 pAb

H, WRWB

200 µl39385

Reactivity Key
D Drosophila
H Human
M MouseR Rat

ChIP, WB, DB 100 µl

Sc Budding yeast
Sp Fission Yeast
WR Wide range

Applications Key
ChIP Chromatin immunoprecipitation
DB Dot blot
ELISA ELISAICC Immunocytochemistry

IFIHC
IPWB

Immunofluorescence
Immunohistochemistry
Immunoprecipitation
Western blot

For an up-to-date list of available histone modification antibodies, please visit www.activemotif.com/hismodabs.

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