Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /0, No. +,, 0-3-0.0 (,**3) ./

 33 



639




EF­³*, ** G´* µ° ¶* ·H x*¸IJK* ¹ º* L»¼Y* M#½N** XYZ[\X** ]^_`a Lb

Antihepatopathic Activity of Foam Components Produced from the Bulb of Jumbo
Leek and Isolation of Its Active Saponin
Ayumi Uchida*, **, Kei Tao*, Jun Ogihara*, Makoto Akao*, Hitomi Kumagai*,
Hiroshi Matsufuji*, Fumio Takenaga* and Hidetoshi Sakurai** Nihon University, College of Bioresource Sciences, +200 Kameino, Fujisawa, Kanagawa ,/,ῌ2/+*** Wadaman Science Co., Ltd., /.0 Karasuma-oike agaru, Nijoden-cho, Nakagyo-ku, Kyoto 0*.ῌ*2./῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍In this study, we examined the e#ects of lyophilized powder of foam components (foam powder)obtained from the bulb of jumbo leek on liver disorders. Using a rat model, acute liver disorders were
produced by D-galactosamine hydrochloride (GalN) administration to induce fulminant hepatitis andchronic liver disorders were induced by ethanol administration. These e#ects were then compared withthose of freeze-dried powder of the whole bulb and curcumin. We also attempted to isolate and identify thesaponin, the estimated active component in the leek. Both foam powder and freeze-dried powder decreased
the induction of hepatocyte necrosis in GalN-induced acute liver disorders and prevented the occurrence ofethanol-induced chronic liver disorders in rats by inhibiting the absorption of alcohol from the stomach.
From the freeze-dried whole-bulb powder, we isolated the known steroid saponin, karatavioside A (C/*H2*O,-,
MW : +*.2).
(Received Jul. ,,, ,**3 ; Accepted Sep. +., ,**3)
Keywords : jumbo leek, foam powder, saponin, alcohol absorption, karatavioside A῍ῌ῏ῌ῎ :       Acd Allium ampeloprasum

K7e9PQ fGQR+<<>. O!RQg

L. h i7cdjk9hl:E "#7$%mn &456789:+ Do;<= >?@? AB GalN

opDqr'7(stu)7OYFv+ wx +0 y

CDEFG+HI5JK9LM  EtOH CDN

C*zfh QFG+I5JK~=C+O,+"#+{|} AB "# *b===-./~=Cp

V0 `V0C 1e9PQY"#f

72+5JKO3PVE `V45=>M

6ῌ07OV0 W7{ 

AAP CD+=75JKYO8+S9 WP

GalN pDq EtOH 7=791CpRQ <

7h

QS7:5JK;!h A7e*1<>90<CHI5JK75 =¡¢O>£Fv+

PQR+S9O¤PV,0 SGf75 =¡¢>£!

!OTP `V¥f7 O8+S9Ch eC¦`G+

DEI5JKOPQR+S9*TUFGV0 WPQ*5§?C¨O1©

QR+9ª^G+ *« C¬RQh@At­+0-

WStBth W7e9PQy

7b®¯

t­+ (,/ R)-"#fCfGVespirost- /-ene-, a, - b-diol- --O-1O-b-D-glucopyranosyl-(+ 27+{|} AB  9°D7"#pDq5J,)-O-[b-D-xylopyranosyl- (+ 2 -)] -O-b-D-glucopyranosyl-* ,/,ῌ2/+*   !"# +200** 0*.ῌ*2./ $ ! $%&'( )*+, /.0 Corresponding author ayumi_u@mue.biglobe.ne.jp  A 7±²OAfCPV7t¤+0(+2.)-b-D-galactopyranoside3 C/*H2*O,- MW : +*.2  ! "#  /0 $  +, % ,**3  +, &640

 34    
+  !"#

' ()*+,-./01234
5657,8

1 &9:1*+,-./0  + kg  + l ;<=70>/?
@ A/B?CD CE;= =FG
H?I9 !J"KL# 9$ +./ -ῌ M *+,-./0  @="KL# 5657,NO%PQ"RS&TU,VW'() 32ῌ 11*X=+8, $%&'()*+,-./0 12",-YZ. -[\ /,-YZ]Z^0 + 



18]_
ῌ ]Z,-234`a2b 0*.

 GalN   0* mgῌkg  + I/ 341 Wistar c SPF 56def 5ghPQ" GalN 78 0* mgῌkg  - I 0 

R 9i j3:;`a ]Zk de

GalNῒ 0*-  GalN  0* mgῌkgf l) ,, + m) // /ῌ n>o56 +,  - I  1  GalNῒ5657, 0*-  GalN<=p8q2b?r>?s@t/*=`au5657, 0* mgῌkg  - Iv wTx def yzx{/`aAB|}`C
CE-, PQ"R-C,{/  CF 

+2 

ῑ EtOH D~E

h6U/66~E6def1? .*ῌ {

/6 EtOH  +  + ml, ., G6Hh6
U/6I



+/ mgῌkg 

῍ ~Edef1 ?234YZ

G3q .*ῌ EtOH + ml ,ῐ GalN D~E

8= CT 8 1 EtOHῒCT

GalN = Do Ad5f>7, wh6

 .*ῌ EtOH 1 YZ ., J,.e+ molῌl 1{/6LM?NOu E>,6

NaOH ?PQ R6{/?CD S ET?

9i 88 K UVW - *** rpm, +/ min, .  ¡X¢6

 GalN  1** mgῌkg  1 Y1def + ml Z£¤1[\X M <def1][\¥^ ' GalN 1_¦  ? 2 ]Z §13q +  1 Y= . `\a1b  % ,.  234_¦  ? -,   +  CT 8  + ml 1  , 

1¨ - I,8=©ª

M

GalN 

EtOH-CT  .*ῌ EtOH  + mlῌday   -  EtOHῒ

'<G]Z`\ *+,-

 +/  .*ῌ EtOH + ml  +/ mgῌ

./012345657, 8 kgῌday   .  EtOHῒ +/  .*ῌ EtOH

  + ml < «c U,+ ml  +/ mgῌkgῌday 

 +/ 

f¬/6 CT 8 1 + ml < W

῎ EO '­[\LQde,Vfgh SPOUCHEMTM EZ SP-

GalN  * 

* 234 .2 ,

{/6LM?iNO3® 1E>,..-* h/5goPQ"R ij 8= UP1h

6 UVW - *** rpm . +/ min  ¡X¢x¯d°,h7fd,x±d/B AST 234h

6 £¤234U{,¯5¤1[\X 2 

d²,h7fd,x±d/B ALT ¢6 k³.6

,{/6LM,/ mg 1´,fµ6³{/6 Qn³, T-Bil l go 6{76fd,x´¶/B

m!·PQ"R.¸  ,* ml 1¬¹g,.U/6

g-GTP ¢6

Lactate dehydrogenase LDHAlka- / ml {/6?K ? /* ml  fill up line phosphatase ALP ¢6n[\ [\0ef,-]Z= +  CT  8 1= xºef»zTM ῏w6~¼oῒ, h/5go ,  GalN-CT   GalN 1** mgῌkg 

PQ"R ij 8

GalNῒ -*--

 GalN  -* mgῌ

kg  - I  .  GalNῒ 0*-  GalN 0* mgῌkg  - I /  GalNῒ- 3 4)5 )6712"
/ 341 Wistar cj3:;`a .*ῌ EtOH 1+ ml ,8= ½ *+,

ῌ : 6789:;6, 35 

<=>

641

"*+,! +/ mgῌkg ?@A

BCD*'EF)# GHI HPLC-RI  - JK .*῍ EtOH + ml (L MN,O'

P7QR, Fr. B-+ Fr. B-, Fr. B-- ' S7T

UVM

# CT !W X EtOH-

CT ! .*῍ EtOH XUVM# EtOH

Y:,Z*!"#! +** ῌl $%! *./ mlῌmin [&!BCD*ῌ 1/ῌ,/ '\ῌ'(# ]^)_' * )_"# + , `@a . )_IbcC,*+$

B-+ ' ,0 mg 

de,-@f *./ ml .-# g/-h:,Z* *.--

῍ ]^ B-+ ijk*0molῌl HW12'EF345 - *** rpm +/ min . ]^ B-+ ! UPLC-MSῌMS, FAB-MS `@aI - EtOH 678l#NMR 0# 3% Fr. B-+ 'C,OT9:mn;

 ! +  . "# $% . &#+  CT !W+ ml X'

EtOH-CT ! .*῍ EtOH ' + ml,

-  EtOH῎'?@#Ar%hBstku7P <=bpCq>( UPLCTM l# LC Cv! [& : *.+῍ 9

 +/ mgῌkg

2wD 1/῍ BCD* S7T : Waters Acquity UPLC

.  EtOH῎*+, +/ ! .*῍ EtOH +BEH C+2 ,.+ /* mm +.1 ῌm $% : *., mlῌmin S7 +/ ! .*῍ EtOH + ml (#

#0o <=bpCq>( Quattro Premier XE"# -.*Tx7 : .*  :,Z*!"# : , ῌl dyz { MSῌ

|*67! }}7o UVmini +,.* EF G~MS Cv! ,]H : ESI ; > 7=ml )#*+, +/ mgῌkg

F῎>kCD* J.K. ,CQR* G~ 'I : -./ kV, ,i : +,*  JKi : .**  8LH!h A M=,2S=bTh +** ml,|,I : .* V |=R,I : .* V JKi :NAD . mg, aldehyde dehydrogenase Al-DH *.2 U - ml

2** lῌh |,i : /* lῌh |=R,i : *.- mlῌmin" +* NO#34 *.+ ml 'P# - ,QI -.* nm 0 # 2 R5 #]^  B-+ ' FAB-MS

STd}7'8# ' E+ "# JI 

<=IUV>( JMS-SX +*,A  WXY"Hh B alcohol dehydrogenase ADH 1*** U *.*/ ml ",]H : FAB ;`@a FAB 8

 P # +*  _ ,Q I -.* nm  ST d} 7 ' 8 #tk=i : u=s* d0# NMR ! Z[ ' E, "# >kt6.*\8#

':B*i*>Q DMSO-d0 `@a:=,' - EtOH 67 E gῌl Z[ E,
E, E+*.++/,

2.32

E+

7,

+* o`V@f8#' 8 # gijk*Qu*! /   Ma . 

b7k" ,<=>
de fa*+,Pyridine-d/ )# ]^0_Q <=IU

V>( ECA-/**  +H- `@a +-C-NMR ijk*



`@a

NMR H@fcL! Dunnet

g:h dio)0# p*.*/ 'D¡"#/ 

+

ῌ R5LH

GalN 

!"#$%&'()*+,-.

`@adewD¢?:;6,Dj! Z[

GalN klb7k-h]£¤¥' m +'Q=S¦*nostku7P §$ TLC 

p# GalNq¨ ss*TῌBCD*ῌ +*ῌ+*ῌ+ # lrs

h© AST ªt`@a ALT ªtD¡3u#

*./ ml .6i.*O«  *.3 ml CD*  *./ ml v

3uw#2 'iZc#¬x¢?i;k@f# :!yz­'p#

;6,{! Mimaki 2LH@f 0*῍ 12 +**BCD*|}'ZX ¯~! +**῍ BCD*d\{'yz/ ¢/² de +., g BC

 .2 )_I! GalN-CT ! CT

 -* - 0* - 0* .

 de`@a*+,0®D¡yz­'p# Z[_d°!±21z
g-GTP ªt`@a T-Bil x]! GalN .2 )

D* - l 'EF|} GH ³;cQR,'´f_I` GalN-CT ! CT h©D¡3u# \:,Z*h'# 3uw#a :,Z*h' p°µo RI '¤}¶,"# HPLC :,Z*  h' '( # S7T!
DAISO-PAK SP-+,*-/ -ODS-AP · V  >  / ml ..0 mm ,/* mm ' # - ' de`@a*+!D¡yz­±2#@?°! 3* +2* `@a ,.* mg dh#^ g"®D¡°¸?® AST ALT d! #¹3452 {# +2* mg e

# 2ºP7QR,'g³;cC1'#&»¼# A  B  C "# 2w#g½  AST ALT T-Bil /012$3452`! e -  B  0/῍9:;:<= > /0 ? > +, @ ,**3  +, A642

 + GalN 



AST

ALT

T-Bil

g-GTP

IUῌl

IUῌl

mgῌdlIUῌl

2.4/ 241..14*224*+234-1+40**+0+4-+*4.**+1,42,+4+**+.141,*4,**+.04.,+4,**+340 +4/-.241214/
0,41,14/**-24++/4+**,/4- ,43**-04* 34-**,*40 ,40***4,,*4*+4/**4.*4,0*4*-***4,+*4*,***4-.*4*-***4,**4*.***4--*4*-**+4-*4-.42*4,/-41*4,*,42*4/2*-4,*4,**-4**4/2*-4**4--**CT
GalN-CT
GalNῌ -*-
GalNῌ 0*-
GalNῌ 0*.
GalNῌ 0*-
GalNῌ0*-

 36 

Dunnet  * p*4*/ ** p*4*+ meanSE n1CT    GalN-CT   GalN 1** mgῌkg
 GalNῌ -*- 
GalN -* mgῌkg  - 
 GalNῌ 0*-  GalN 0* mgῌkg
- GalNῌ 0*.  GalN 0* mgῌkg  +  GalN  0* mgῌkg  - 
 GalNῌ 0*-  GalN 0* mgῌkg  - GalNῌ0*-  GalN
0* mgῌkg  -  ,EtOH 
AST

ALT

T-Bil

g-GTP

IUῌl

IUῌl

mgῌdlIUῌl

1-4- +4,+*14- 34/004/+.4***
1.42 241**+.4.+41,34*.4+.4,*42*+-42,4***4-.*4*,*40**4**4.**4*1*4.0*4*.

*42*4,*.4-+4,*+42*4+1**+42*4,***CT
EtOH-CT
EtOHῌ +/
EtOHῌ +/Dunnet * p*4*/ ** p*4*+meanSE n1

B CD34  EF4 )*+/SF/ T4FUVWXYZ/` 4FUB3/5678
noU3

 +2* mg pq/ AST ALT

rsE

F4 &'( /vew,8/ g-GTP EF4
x/w,8yuH
#$IJK "#$ .oUB3/,uyH 0

/^U AAP

"#$IJK.} E^ Reddy ,/X^U!"#$IJK,.}. 3#4F
I K)! "#$% &'( 
3

.

!)*+

3 0 tbYa^U_aM NOPQRu,

TNF-a -,z{ |} Rj klQm  GalN "
4 B

1308^H4-78U_

abc/defgH4Eh i Rj klQm

0

3 

GH4 IJK "#$EF4L "M NOPQR[\U Kupffer ]

./0 ( 12345678

EtOH 



 !"~ Q a"#$ IJK EtOH 3Rj
klQm |}  $c/ye F8 L 33L 
[ xp3",8 3Ly/,8"#$zu,KXV a"EtOH "#$IJK¡Y:¢ |}$ ,# £¤¥¦oUB3/§,84FU/ ¨F1©o EtOH

2f4ª784FyF «)VK¬VCT ¯4°±s %²[\U/ AST _aE^/³I K)U­®

^h "M NOPQR[\

J·Q Kupffer ]u,

TNF-aoU[yFu3¹º,84FU

1

&'/(³

¸I K)"#$  » K¼m½ /f  » K¼m½ M NOPQR_aY )VK¬V'f 4FU3 /\U2&

^h .,

 EtOH-CT 

ALT _a/´{µ B {µ¶4 

E^´y .}/,8 ¸ ~ Q #$MQ¬Q[\U g-GTP _a ., EtOH-CT  CT ¯ ´{µ B{µ¶4 .

T-Bil s .,E^
´

EtOH-CT /°±sῌ5 : 6 78-9:;7   37  
* hCT
EtOH-CT
EtOHῌ +/
EtOHῌ
+/

*
*
*
*

 EtOH + h*4**-2+433+4/3++4/+1

Dunnet  * p*4*/ ** p*4*+*4*+-1,4,*.
+40/+4/-.

*4**0**4,-/*4*-+**4-,**

mgῌdl. h*4**,.
+4131
+4*00
*4330

*4**,**4,30*4*,1**4,/3*

mean SE n.

 +  B-+<=$ CT >?@AB



, h

*4**,*
*4*12
*4++3
*4,0+

643

C@ADB$

UPLC-MSῌMS 

EFGH$#C'IJKLM?NMOPLHQ' R.  !"  #$%&'STIB

PLM UV WXY3

CLP]^QP Z_ `a3bM
hiEFjBk l3 hiEFDB$mnG
'C'B- 

\cdbe4fB ghB +., g QPopKL A B C :;7 q
M +.+ g r sB B & - tu3 Mvwx
B$y!,0 mg ++ mg "+, / mg  EtOH  

EtOH 8z{"|%& EtOH  }(~ - I  ZW[

#M :;7 M Z_ `a3b  +.3- gῌkg wet wt  Fr. B-+  UPLC-

"#$ %& EtOH  ' + ()* EtOH-CT

MSῌMS BC

B$

pKL !u3 M mῌz +*.3 MH 

. ()*M-*῍ , ()*,1῍ ?B.*῍ IB O# "ST'KL
f#^hOC'$KL$#+* -.3 H MS u3'u3 IB MSῌMS ]^ mῌz 221 1// /3- "+Mi, .,3 c8W { u3 IB $ !

~%M  + I+ l3\ Glc B

EtOH RG ¡M &¢:;7 M EtOH '£¤¥C'IJKL ¦§ῌsB~

¨QP©ª«~¬­FGB$$K% B-+  FAB-MS BC  ;Z/01234

L$#++

®(¯#C { 1a)*:;7 "#$ mῌz +*.3 MH "+,-./01234 mῌz

MsKLu3 +k l3 RG'°H

+*.1 M-H  u3 IB !~%

,'$KL$#±²M +*.2 C/*H2*O,- r UPLC-MSῌMS ]^+,@³CQP Z_ `a3bM:;7´µB

'qL i EtOH f¶·Q ¨QP NMR ]^M¸¹r

EtOH ­FB$#C'º»KL

+

G'k

l3

¼B$ ChiEFjB EtOH ½i

H-NMR /** MHz, DMSO-d0, d ppm : *.1* s, -H, Ag-

H+2 *.1+ d, -H, J0.* Hz, Ag-H,1 *.20 m, +H, Ag-56789:9;< = /0 > = +, ? ,**3 @ +, A

644

 .





Solvents
AglyconDMSO-d0+

Pyridine-d/

Position

+
,
.
/
0
1
2
3
+*
++
+,
++.
+/
+0
+1
+2
+3
,*
,+
,,
,,.
,/
,0
,1

./41
1*4*
2-4+
-14+
+.*4,
+,+41

-,4*
-+4*
.343
-142
,+4*
-340
DMSO-d0
/04,

-,4*
2*41
0,4+04/
,*40
.+40
+/4,
+*243
-+4/
,34*
-*4
004/
+140

./41
1*42
2.40
-141
+.*4+
+,+43

-,4,
-+4+
/*4,
-143
,+4,
-342
.*4/
/04/

-,4,
2+4,
0,43
+04,*4.
.,4*+/4*+*34-+42
,34,
-*40
0043
+14-

CH,
CH OHCH OCH,
C CHCH C
CH,
CH
CH
C
CH,
CH,
C
CH
CH,
CH O
CH
CH- C
CH- CCH CH-
CH- CHC ,O
CH,
CH,
CH CH-
CH, O
CH- CH 38 

 +-C-NMR 
Solvents
Sugar
Galῌ
+
,
.
/
0
Glc
+
,
.
/
0
Glcῌῌῌ
+,.
/0Xylῌῌῌῌῌ+,.
/DMSO-d0+

Pyridine-d/

Position

+*+42
1+40
1.4+
1341.41
0*4+

+*-4.
1,40
1/4/
1341/41
0*4/CH ,OCH OHCH OHCH O
CH O
CH, OH

+*-4*
1343
2/41
034,
104/
0+43

+*.41
2+4+
214*
1*4*
1140
0-4*

CH ,OCH O
CH O
CH OHCH O
CH, OH

+*-41
1.42
1041
1*4.
114+
0+40

+*.42
104+
124+
1+4.
124.
0,41

CH ,OCH OHCH OHCH OHCH O
CH, OH

+*-43
1.4*
114034
004/

+*/4*
1/4+
1241
1*4.
014.

CH ,OCH OHCH OHCH OHCH, O+DMSO-d0 : dimethyl sulfoxide-d0 CD-, SO  +-C-NMR   DEPT HSQC 

HMBC 

H+a* *.22 d, -H, J1., Hz, *.3* m, +H, Ag-H3 *.30-.// m, +H, Glcῌῌῌ-0a* -./0 q, +H, J2./ Hz, Glc--s, -H, Ag-H+3 +.*1 m, +H, Ag-H+. +.+* m, +H, Ag-H-.02 m, +H, Galῌ-0b* -.02 m, +H, Glcῌῌῌ-0b* -.03 m,+,a* +.+. m, +H, Ag-H+/a* +.,2 m, +H, Ag-H,.a*

+H, Glc-0b* -.1- m, +H, Ag-H,0b* -.11 m, +H, Gal-+.-1 m, +H, Ag-H++a* +../ m, +H, Ag-H,-a* +..1

. ..+3 d, +H, J1., Hz, Gal-+ ..,/ q, +H, J1., Hz,

m, +H, Ag-H++b* +..1 m, +H, Ag-H+/b* +./* m, +H,

Ag-H+0 ...* d, +H, J1.1 Hz, Glcῌῌῌ-+ ...1 d, +H, J

Ag-H2 +./* m, +H, Ag-H,/ +./+ m, +H, Ag-H1a*

1.2 Hz, Xylῌῌῌῌ-+ ..02 d, +H, J2.* Hz, Glc-+ /.-++./+ m, +H, Ag-H,.b* +.0* m, +H, Ag-H,-b* +.0/

d, +H, J..* Hz, Ag-H0 * BCDE FGHIJIm, +H, Ag-H+,b* +.00 m, +H, Ag-H+1 +.13 brq, ++-H, J1., Hz, Ag-H,* +.21 m, +H, Ag-H1b* +.3+ m,

C-NMR DMSO-d0 

Pyridine-d/

K . 3DMSO-d0 LM C-NMR NG3IH  .2 
+-+H, Ag-H+b* ,.,* brt, +H, J+- Hz, Ag-H-a* ,.-+OPQRSJOTU DEPT dd, +H, J../, +- Hz, Ag-H-b* ,.3. t, +H, J2.-, Galῌ-

G3

H/ -.*, m, +H, Galῌ-- -.*1 m, +H, Xylῌῌῌῌ-/a -.*2

` JTHI.a` 

m, +H, Glcῌῌῌ-/ -.*3 m, +H, Xylῌῌῌῌ-. -.+* m, +H,,\]^,` Xyl b+cd C/ YZ[,\]^,`Ue

Glcῌῌῌ-. -.++ m, +H, Glcῌῌῌ-- -.+, m, +H, Xylῌῌῌῌ--f3-.+1 m, +H, Ag-H- -.+1 m, +H, Ag-H,0a* -.+3 m,

 DMSO-d0  NMR m]i3n.op+H, Glc-/ -.,. m, +H, Ag-H, -.-, m, +H, Gal-,

qrsWt, od/ 3uLv NMR m]i-.-- m, +H, Glc-0a* -.-- m, +H, Xylῌῌῌῌ-, -.-0 m,o`ef3wx+H, Xylῌῌῌῌ-/b -.-2 m, +H, Galῌ-0a* -.-2 m, +H, Glcῌῌῌ-+

H-NMR, +-C-NMR , -./- d, +H, J1.2 Hz, Glc-, -./- m, +H, Glc-.

+-

HMQC N

4VWX, C +- YZ[,\]^,UL_
VWX, C,0 YZ[

JTHI.a`Ugh \ijkl)'( A
JIU "

/0 , 12 NMR 3 4

 ! "#$%&'()*+,-. (,/R)-spirost-ῌ= : >?@A < 39 

645

 ,  B-+ /-ene-, a, - b-diol- --O-O-b-D-glucopyranosyl-(+  ,)-O-[b-B  ,,./ gῌkg  C*+ -DD-xylopyranosyl-(+  -)]-O-b-D-glucopyranosyl-(+  .)-b-

EF)G12+,-)  HIJ*3D

D-galactopyranoside KL?MNOP A 4-.

EF&QR+STU9 $VG&W-.

)*3 X , YZ[ \K?LM]^ )_`a- b3 *3'c B dae%&+0 : f? &12+,-.)g12+,

A dhei . j!)G12+#V HIklῌ-+. )  !"hem +4-

noJῌ <p+st  !"uvwxyz9GW- 5 6& 7 8 9:;<*

4-.{  !"|}6&~&$
W-  !)12- 9*3 GalN #$% EtOH %$uvw?x&st&#,+ 8 5a 
4V  0* mg 9 -  *3 +2* mg d +... mgῌkg  -* mg 9 - !"qr#

 !"he'e23C3 &*3 3* mg

#$%9,+ GaIN &$V%$12-H) +., g he *3  +.+ g 

?&W-yz93 b3 6& /<7 /02 g &12+,-3

8 *+heJῌ <9s3 d - 0 mgῌkg a- !" .
,Fuvw#$% EtOH &$+12-(Fuvw *.+3-῍ 

+/12+,-sa3 *h* :  !"#$% GaIN
#$% EtOH ?x

t&#,+Fuvw

|}&#,+"q12+,-he/0W- 

6T* 9&W-. b3¡¢!£¤9&& ,'()*+,-.)/0123

W-.&$V(Fuvw9+,W-.9eh&*

KOK >-#$%¥ ¦ ¦3 *+ 7 8 *+no§¨©OP?&W-uvwª«)g12+,

4- (,/R)-spirost-/-ene-,a, -b-diol---O-O-b-D-gluco-- Wa¬­ KOK /* ῌgῌml +** ῌgῌml

CCL. 

pyranosyl-(+  ,)-O-[b-D-xylopyranosyl-(+  -)]-O-b-D-glu-

 &$V AST  ALT ®F ) e2+,-

copyranosyl-(+.)-b-D-galactopyranoside C/*H2*O,-*h* ¯& /** ῌgῌml °, &#,+.F)±

MW : +*.2 K?LMNOP A ²³9´µ*3

¶12+,--  !" · F.F
LD/*  ?x/¸ &#,+ +2.*- gῌkg ¹º0 18 +3 234>»¼½\»0

Et  /0   +,  ,**3  +, 646 ¡¢T£s¤ ¡¥¦-§< ¨©Uª«
῍
+

,

-

.

/

0

1
2

3

ῌ

+*

Ariga, T., Kumagai, H., Yoshikawa, M., Kawakami, H.,
Seki, T., Sakurai, H., Hasegawa, I., Etoh, T., Sumiyoshi,
H., Tsuneyoshi,T., Sumi, S. and Iwai, K., Garlic-like but
Odorless Plant Allium ampeloprasum Mushuu-ninniku’,J. Japan. Soc. Hort. Sci., 1+ (-), -0,ῌ-03 (,**,).
  ! " #$ % &'( )*+, -./0123456789:;<=>?@A   // /.3ῌ//2
,**2
Miyao, H., Arao, T., Udayama, M., Kinjo, J. and Nohara,T., Kaikasaponin III and Soyasaponin I, Major Triterpenesaponins of Abrus Cantoniensis, Act on GOT
and GPT : Inﬂuence on Transaminase Elevation of Rat
Liver Cells Concomitantly Exposed to CCl. for One
Hour., Planta Med., 0. (+), /ῌ1 (+332).
Reddy, A.C.P. and Lokesh, B.R., E#ect of Curcumin andEugenol on Iron-induced Hepatic toxicity in Rats, Toxicology, +*1, -3ῌ./ (+330).
Mimaki, Y., Kuroda, M. and Sashida, Y., Steroidal
Saponins from the Bulbs of Allium ampeloprasum. Natural Medicines, /- (-), +-.ῌ+-1 (+333).
 B! " CDEFG HI J #$ %
)*+, KLMN -./0123>O9PQRSTKUVTW>XYZ[\ E /0 ,2*ῌ,2/ ,**3
]^ _ `abcd efg hi jVk1l/>mno KVXp q'rst ++2 3+ῌ33 ,**-*uvw x$yz {|}~ /R<3l/ hi V Kupffers >K>Xp E t ,/ 3,-*+ῌ,-*1 +33,
Tran, Q.L., Adnyana, I.K., Tezuka, Y., Saiki, I.,
Kurashige, Y., Tran, Q.K. and Kobata, S., Hepatoprotective
E#ect of Majonoside, the Major Saponin from Vietnamese

++

+,

+-

+.

+/

+0

 40 

Ginseng (Panax vietnamensis). Planta. Med., 02, .*,ῌ.*0(,**,).
Okada, K., Wangpoengtrakul, C., Tanaka, T., Toyokuni,
S., Uchida, K. and Osawa, T., Curcumin and Especially
Tetrahydrocurcumin Ameliorate Oxidateive Stressinduced Renal Injury in Mice, J. Nutr., +-+, ,*3*ῌ,*3/(,**+).
Tsukamoto, S., Kanegae, T., Nagoya, T., Shimamura, M.,
Kato, T., Watanabe, S. and Kawaguchi, M., E#ect ofSeed Saponins of Thea sinensis L. (Ryokucha Saponin)
on Alcohol Absorption and Metabolism. Alcohol andAlcoholism, ,2, 021ῌ03, (+33-).
Yoshikawa, M., Shimada, H., Morikawa, T., Yoshizumi,
S., Matsumura, N., Murakami, T., Matsuda, H., Hori, K.
and Yamahara, J., Medisinal Foodstu#s. Vll on the
Saponin Costituents with Glucose and Alcohol Abosorption-Inhibitory Activity from a Food Garnish ‘Tonburi’,
the Fruit of Japanese Kochia scoparia (L.) Schrad,
Kochia scopavia : Structures of Scoparianoside A, B andC, Chem. Pharm. Bull., ./, +-**ῌ+-*/ (+331).
Macias, F.A., Guerra, J.O., Simonet, A.M. and Nogueiras, C.M.,
Characterization of the Fraction Components using +D
TOCSY and +D ROESY Experiments. Four New Spirosane
Saponins from Agave brittoniana Trel. spp. Brachypus,
Magn. Rcson. Chem., ./, 0+/ῌ0,* (,**1).
Mimaki, Y., Kuroda, M., Fukasawa, T. and Sashida, Y.,
Steroidal Saponin from the Bulbs of Allium karataviense, Chem. Pharm. Bull., .1 (0), 1-2ῌ1.- (+333).
Fenwick, D.E. and Oakenfull, D., Saponin Content ofFood Plants and Some Prepared Foods, J. Sci. Food Agric.,-., +20ῌ+3+ (+32-).
 />S >o Food Style, ,+ (,), ./ῌ.2 (+332).

¬T ,+  1  ,, E­® ¬T ,+  3  +. E­¯ῌῌῌῌῌῌῌῌῌῌῌῌῌῌῌῌῌῌῌῌῌῌῌῌ