COINS Seminar #47【Date】Aug/28/2019（Wed.）15:00～16:30（Registration Open at 14:30）【Venue】Innovation Center of NanoMedicine (iCONM) 3F 3001 Meeting room
【Registration】URL: https://www.cis-trans.jp/coins_seminar47/index.html◆Part.1

15:00～15:45

Title:Cell Lego

Abstract:
Techniques to control the spatiotemporal organization of cells are primarily limited to homogeneous
cell populations. We have previously explored synthetic single stranded DNA-PEG-lipid conjugatesas a mean to introduce artificial binding capacity to cells by exploiting specific hybridization ofDNA. DNA is an attractive ligand partly because of its specific binding affinity and the capacity fora practically unlimited variety of binding interaction schemes. This technology was extended to avariety of applications including the co-localization of unlike cell types and printing of cell patterns
on 2D substrates. In this presentation, its potentials in regenerative medicine and biomedical devices
will be discussed.
Speaker:

Hiroo Iwata, Dr. Eng.

Affiliation: Kyoto University
Position: Manager, Strategy Research Support Section of Centerof Innovation Program, Kyoto U.

＜CV＞
1980~1982
1983~1994
1994~1999
1999~
2011~2015
2015~

Research associate at the Departmentof Material Science, University ofFlorida.
Research scientist of National
Cardiovascular Center Institute, Osaka
Associate Professor, Research Center for Biomedical Engineering, Kyoto U.
Professor, Department of Reparative Materials, Institute for
Frontier Medical Sciences, Kyoto U.
Director of Institute for Frontier Medical Sciences, Kyoto U.
Manager, Strategy Research Support Section of Centerof Innovation Program, Kyoto U.

＜Key words＞
Biomaterials, Islet transplantation, Devices for interventional neuroradiology◆Part.2

15:45～16:30

Title:Isolation of Rare Cells: Toward Noninvasive Diagnostic
Abstract:
In this lecture, I will discuss the recent developments in our group related to the isolation of
circulating tumor cells (CTCs) and circulating fetal traophoblasts for noninvasive diagnostic tools.
We have employed a novel one step electrospinning process to fabricate poly(ethylene oxide)(PEO)/ poly (3,4- ethylenedioxythiophene): polystyrenesulfonate (PEDOT:PSS) core/shell
nanofiber structures with improved water resistance and good electrochemical properties. We thenintegrated a biocompatible polymer coating with three-dimensional (3D) PEDOT-based nanofiber
devices for dynamic control over the capture/release performance of rare circulating tumor cells(CTCs), as well as the label-free detection by using organic electrochemical transistors (OECTs).
We have demonstrated that these nanofiber mats deposited on the patterned indium tin oxide finger
electrodes are excellent candidates for use as functional bioelectronic interfaces for the isolation,detection, sequential collection, and enrichment of rare CTCs through electrical activation of eachsingle electrode. This combination behaved as an ideal model system displaying a high cell-capture
yield for antibody-positive cells while resisting the adhesion of antibody-negative cells. Taking
advantage of the electrochemical doping/dedoping characteristics of PEDOT:PSS materials, thecaptured rare cells could be electrically triggered release through the desorption phenomena of PLLg-PEG-biotin on device surface. More than 90% of the targeted cancer cells were captured on the3D PEDOT-based nanofiber microfluidic device; over 87% of captured cancer cells weresubsequently released for collection; approximately 80% of spiked cancer cells could be collectedin a 96-well plate. I will also discuss the utilization of multi-photon microscopy for monitoring
CTCs in the blood stream. The number of CTCs has been used as an indication for the progress oftumor state. However, how CTCs travel in the bloodstream and how they crossed the endothelial
barrier are not known. In our group, we have utilized the multi-photon microscopy to study CTCs
noninvasively. Pancreatic cancer cells expressing fluorescence were subcutaneously injected to theearlobes of mice forming solid tumor. When the cancer cells break away from the tumor mass, thecancer cells in blood stream can be monitored. The number of CTCs observed in the blood vessels
near the tumor mass increased to a maximum value after five week of inoculation. We also tried toidentify a sub population of CTCs such as cancer stem cells (CSCs). The trajectories of CTCs andCSCs were measured and analyzed.
Speaker:Peilin Chen
Affiliation:Research Center for Applied Sciences,Academia Sinica, Taiwan
Position:Professor/Research Fellow
URL:http://www.rcas.sinica.edu.tw/faculty/peilin.html＜CV＞
Peilin Chen received his Bachelor degree in Chemistry from National Taiwan University in 1990
and obtained his Ph.D. degree in Chemistry from University of California, Irvine in 1998. Heworked as a postdoctoral fellow in the Chemistry department of University of California, Berkeley
between 1999 and 2001. Prof. Chen joined Research Center for Applied Sciences, Academia Sinica,
Taiwan as an Assistant Research Fellow in 2001. He was promoted to Associate Research Fellow
and Research Fellow in 2005 and 2010, respectively. He served as the deputy director of theResearch Center for Applied Sciences between 2010 and 2012 and the Chief Executive Officer ofthe thematic center of Optoelectronic in 2012. Prof. Chen was a visiting Professor in RIKEN andKyoto University. Prof. Chen has authored or co-authored more than 120 papers in refereed journals
and conference proceedings, he has delivered more than 60 invited talks in international meetingsand conferences. He organized more than 10 international symposia.
＜Award＞
Research Award for Junior Research Investigators, Academia Sinica, Taiwan (2007)
Ta-You Wu Memorial Award, National Research Council, Taiwan (2007)
Career Development Award, Academia Sinica, Taiwan (2009)＜Key words＞
Nanofiber, Circulating tumor cell, Bioelectronic, Intravital imaging*Supported by: COINS（JST, COIProgram）, Innovation Center of NanoMedicine（iCONM）