Vanquish Duo HPLC and UHPLC Systems

Maximize productivity

FPO - would like to use a
different angle of Duo like shownhere

2

Doubled productivity with
2 UHPLC systems in one stack

Optimize your productivity

Vanquish Duofor Dual LC

In today’s challenging research andproduction environments, you arebeing asked to be more productive
without increasing spending orcompromising quality.

Uniform
responsewith
ChargedAerosol
Detector

VanquishDuo forInverseGradient

At the same time, advancements andVanquishDuoVanquishDuofor Dual
LC-MS

Integrated
multichannel
LC forultra highthroughput
LC-MS

Vanquish Duo
for Tandem LC or LC-MS

innovations are needed to improve
operational efficiency and performance.

Increased throughput
with LC or LC-MS

How do you accomplish this? It’s easy with
the Thermo Scientific™ Vanquish™ Duo HPLC and UHPLC systems
built for enhanced productivity. By combining individual Vanquish
components, four workflows consisting of distinguished system
configurations and software wizards can be created.

3

Your laboratory may be facing increased pressure
to improve working efficiency and deal with limiting
resources, such as budget, space, or manpower.
Vanquish Duo HPLC and UHPLC systems are designed
to overcome these limitations.
Vanquish Duo HPLC and UHPLC systems consist of fourindividual workflows, each separately configured,to address specific analytical challenges.

Initial investment without detector

Two flow paths for maximized productivity
Dual LC-MS
Dual LC

Tandem LC or LC-MS

Inverse Gradient

Productivity

Unique dual technology
The Vanquish platform contains two unique dual
modules. The Vanquish dual pumps merge two
individual, low pressure mixing pumps in one
housing enabling separate eluents and gradientsfor each pump.
The Vanquish dual split samplers possess two
independently controlled injection units including
separate injection valves and injection needles.
Used in Dual LC and LC-MS workflows only, thisenables high throughout and extensive sample
characterization workflows.

4Individual configurations to best serve your needs
Vanquish Duo workflows are configured with Thermo
Scientific™ Vanquish™ Core system, Thermo Scientific™
Vanquish™ Flex system or Thermo Scientific™ Vanquish™
Horizon system modules. All four of these workflows
make use of two pumps to increase the analyticalproductivity.

Vanquish Duosystem for Dual LC

Vanquish Duosystem for Dual
LC-MS

Vanquish Duosystem for Tandem
LC or LC-MS

Vanquish Duosystem for Inverse
Gradient

Supported
systems• Vanquish Horizon• Vanquish Flex

• Vanquish Horizon• Vanquish Flex

• Vanquish Horizon• Vanquish Flex
• Vanquish Core

• Vanquish Horizon• Vanquish Flex
• Vanquish Core

Sampler

Dual split sampler

Dual split sampler

Split sampler

Split sampler

Detector

Two out of thecomplete detector
portfolio

One mass
spectrometer

One out of thecomplete detector
portfolio and/or one
mass spectrometer

Thermo Scientific™
Vanquish™ Charged
Aerosol Detector

Software control• Thermo Scientific™
Chromeleon™
Chromatography
Data System (CDS)• Thermo Scientific™
Standard Instrument
Integration (SII) for
Waters™ Empower™
3 CDS

Thermo Scientific™
Aria™ MX software• Chromeleon CDS• Standard System
Integration for
Thermo Scientific™
Xcalibur™ software•C hromeleon CDS• Thermo Scientific™
Standard Instrument
Integration (SII) for
Waters Empower 3
CDS and Agilent™
OpenLab™ CDS

5

Vanquish Duo System for Dual LC
Single Channel LC

Analytical GradientApplication 1

Flow Path 1

Reconditioning

Analytical GradientApplication 1

Reconditioning

Application
Switch Time

Analytical GradientApplication 2

Reconditioning

Analytical GradientApplication 2

Reconditioning

A VanquishTandem
Duo LCsystem
• Improve return on investment by reducing costor LC-MSfor Dual LC enables you to run
Analytical
Gradient
Analytical
Application doubling
Analytical Gradient
Analytical Gradient
twoFlowmethods
simultaneously
onGradient
one instrument,
per sample
Path
1 Channel
SingleLC
Time savings
Application 1
Application 1
Switch Time
Application 2
Application 2
yourthroughput
ordeepening
yoursampleknowledge
Analytical
Gradient
RecondiAnalytical
Gradient
RecondiApplication
Analytical
Gradient
RecondiAnalytical Gradient
RecondiFlow Path 1
RecondiRecondiRecondiRecondiComparedto single
channelLC
Flow Path 2 Application 1
tioning
Application 1
tioning
Switch Time
Application 2 sample
tioning
Application2
tioning•
Increasethroughput
withoutchanging
tioning
tioning
tioning
tioning
while maximizing your productivity and performance.
validated methods
The unique Vanquish Duo system for Dual LC will:
Tandem LC or LC-MS
Dual LC-MS
Analytical Gradient
Analytical
Gradient
Application
Analytical Gradient
RecondiFlow Path 1
Analytical Gradient
Analytical Gradient
RecondiFlow Path 1 Application
1
Application
Switch Time
Application 2
tioning 1
Application 1
Application 1
tioning

Flow Path 2 RecondiFlow Pathtioning
2

Resulting

RecondiAnalytical Gradienttioning
Application 2

Application 1

Application 2

MS Signal
Dual LC-MS
Flow Path 1

Analytical GradientApplication 1

Flow Path 2

Dual LC

Flow Path 1

Reconditioning

Reconditioning

Analytical GradientApplication 1

Flow Path 1

Analytical GradientApplication 1

Reconditioning

tioning

Tandem LC or LC-MS
TimeTimesavingssavings

Reconditioning

Analytical Gradient
Analytical GradientApplication
Compared tosinglechannelLC
to single
Application 1Compared
Application
1 channel LC
Switch Time
RecondiAnalytical Gradient
RecondiRecondiReconditioning
Application 2
tioning
tioning
tioning

Flow Path 1

Reconditioning

Application 2

Analytical GradientApplication 1

Reconditioning

Reconditioning

ResultingSingleChannelAnalytical
Gradient
Analytical
Gradient 2
1LC
Application 2RecondiApplication
1
Application
Flow Path
2 Application
MS Signal
Application 2

Time savings• Analyze the same set of samples
with multiple methods
Compared to single channel LC
Single Channel LC
reducing sample preparation time and maximizing
Analytical Gradient
RecondiAnalytical Gradient
RecondiApplication
Flow Path 1
Application 1
tioning
Application 1
tioning
Switch Time
sampleknowledge

Reconditioning

Analytical GradientApplication 2

Reconditioning

Reconditioning

tioning

Application 2

Analytical GradientApplication 1

Analytical GradientApplication 2

Analytical GradientApplication 1

Application 1

Time savings

Application 2

Compared tosingle channel LC processdevelopment
Recondi- • Accelerate your method

AnalyticalRecondiGradienttioning
Application
2

Reconditioning• Increase
capacity without sacrificing bench space
Analytical Gradient

Analytical Gradient
Flow Path
Application
22

Application
Switch Time

Analytical GradientApplication 2
Reconditioning

Dual LC
Inverse Gradient LC — Improved quantification with CAD
Analytical
RecondiAnalytical
Gradient RecondiFlow1Path
1TandemLCGradientor LC-MS
Flow Path
Analytical
Gradient
RecondiAnalytical Gradient
RecondiApplication
Application1
tioningtioning Application1
tioningtioning
Application 1
Application 1
Switch Time
Flow PathAnalytical2
Gradient
Analytical
Gradient
Application
Analytical
Gradient
Flow Path 1
Gradient
RecondiAnalytical Gradient
RecondiFlow Path 2 Analytical
Application 1
Application 1
Switch Time
Application 2
Application 2

Flow Path 2

tioning

Reconditioning

Application 2

tioning

Reconditioning

Reconditioning

Flow Path 2
Flow Path 1
Flow Path 2

Analytical Gradient
Dual
LC-MS

Reconditioning
Reconditioning

Application 1
Analytical GradientApplication 1

Dual LC

Analytical GradientApplication 1
Analytical GradientApplication 1

Compared
to single
channelLC
Analytical
Gradient
Recondi-RecondiFlow Path 2
tioning

Inverse Gradient LC — Improved quantification with CAD
Flow Path 1

Analytical Gradient
RecondiGradient
RecondiTime Analyticalsavings
Dual LC-MS
Application 2
tioning
Application 2
tioning
Analytical Gradient
RecondiAnalyticalCompared
GradientTime
Analytical
Gradient
Recondito
singlechannelLCsavings
Application
Flow
Path 1 2
tioning
Application 1
Application 1
tioning
Application 2

Resulting

Reconditioning
Reconditioning

Application
Switch Time

Analytical GradientApplication 2

Reconditioning

Analytical
Gradient
MS Signal
Application 2

Application
Recondi- 1
tioning

tioning

Analytical GradientApplication 2

Application
2
Application
1
Analytical
Gradient
RecondiApplication 2
tioning

Reconditioning

Application 2

What can you do with a Vanquish Duo system for Dual LC?

Analytical GradientApplication 2

Reconditioning

Analytical GradientApplication 1

Reconditioning

Analytical GradientApplication 1

Reconditioning

Application 1

tioning

Application 1

tioning

Flow Path 1
Resulting
1 GradientApplication
2
Application
1 GradientApplication
2
Analytical
RecondiAnalytical
RecondiFlow Path 2 Application
MS Signal

Dual LC–OR–

Flow Path 1

Analytical Gradient Recondi- Analytical Gradientsavings Application 1
Application 1 Timetioning

Reconditioning

Flow Path 2

channelLC Gradient
AnalyticalCompared
Gradient to single
RecondiAnalytical
Application 2
tioning
Application 2

Reconditioning

Run two identical columns
Run two identical columns
Run your complementary
Run your complementaryRun
complementarycolumnsin parallelin parallelin parallelcolumnsinyourparallelcolumnsin parallel
Inverse Gradient
LC — Improved
quantification with CAD

Dual LCRun two identical columns in parallel
Flow Path 1

Analytical GradientApplication 1

Recondi-Flow Path 2

Analytical Gradient
Gradient
Analytical
Application 12
Application

Recondi-Analytical
Analytical
Gradient
RecondiRecondiGradient
Reconditioning
Application
2 tioning tioning
tioning
Application1

tioning
50 samples

Analytical GradientApplication 1

Recondi-Inverse Gradient
LC — Improved quantification
with CAD
Left channel
Left channel
RightchannelFlow Path 1
Flow Path 2

50Gradientinjections
Analytical
RecondiApplication 1
tioning

Flow Path 1
Analytical GradientTime
RecondiAnalytical Gradientsavings
50 samples
100
samplessamples Application 1
Application 1 100 tioning
Flow Path2

tioning
50 samples
50 samples

50Gradientinjections
50 injections
Analytical
RecondiApplication 1
tioning

Reconditioning

Compared to single channel LC

RightchannelLeftchannelLeft
RightchannelchannelRight channel

injections
100 50injections
100
injections
injections
Application
Analytical Gradient100
RecondiAnalytical Gradient100 injections
RecondiSwitch Time
Application 2
tioning
Application 2
tioning

Dual LCBe finishedBe finished in
Be finishedin halfin
the time
half the time

half the time

Dual LC

6

Flow Path 1

Analytical GradientApplication 1

Reconditioning

Analytical GradientApplication 1

Reconditioning

Flow Path 2

Analytical GradientApplication 1

Reconditioning

Analytical GradientApplication 1

Reconditioning

Flow Path 1

Analytical GradientApplication 1

Reconditioning

Analytical GradientApplication 1

Reconditioning

Get moreGet
information
more information
more Get
informationout of every sample
Flow Path 2 Analytical Gradient Recondi- Analytical Gradient RecondiApplication
tioning sample
Application 1
tioningout of every sample
out1 of every

Application
Switch TimeRun your complementarycolumns in parallel
100 samples
40

Thermo Scientific™ MAbPac™
SCX-10 RS column

n=10

Right channel
100 injections

n=10
50

Sample injected on
two complementarycolumns

SCX
Charge Variant Analysis

Channel L

20

Left channel
100 injections

Bevacizumab

mAU

60

SEC
Protein Aggregate Analysis

Channel R

Thermo Scientific™ MAbPac™
SEC-1 column

Bevacizumab

100
0

5

10

15

min
20

25

30

Get more informationout
every sample
Twoof
independent
assays run simultaneously from a single sample on one LC instrument to deepen your sample knowledge.

Initial investment without detector

Dual LC-MS
Dual LC“The instrument offers a fantastic increase in throughput, either whilerunning the same separation chemistry or most excitingly when runningtwo different separation chemistries, enablingTandem
us togenerate large
LC or LC-MS
amounts of characterization data from the same sample quickly.”	—Dr. Jonathan Bones, Principal Investigator, National Institute for Bioprocessing Research and Training
Inverse Gradient

Productivity

Complete control
with just a few clicks
Chromeleon CDS delivers industryleading ease-of-use for the Vanquish
Duo system for Dual LC.
Independently control the twochannels of the Vanquish Duo system
for Dual LC from a single interface
with full traceability of all actions thatis 21 CFR Part 11 compliant.

Easy-to-use and intuitive system control of the Dual LC workflow within Chromeleon CDS.

7

See the Vanquish Duo System for Dual LC
The Vanquish Duo UHPLC system for Dual LC has been built to utilize a wide range of analytical detectors,
including mass spectrometry (MS), diode array detection (DAD), charged aerosol detection (CAD), variablewavelength detection (VWD), and fluorescence detection (FLD).
1

2

3

4

5

6

8

7

Easier operation
Patented tool-free Thermo
Scientific™ Viper™ Fingertight
Fittings with near-zero deadvolume operation andergonomic design.

8Unique Thermo Scientific™ SmartInjecttechnology
Superior retention time precision and enhanced
column protection with intelligent SmartInjecttechnology on both flow paths.

9

Two proprietary injection valves
Two separate injection valves with no shared fluidics
allowing full application flexibility, including individual
eluents, wash liquids and loop sizes.

10

Easier sample handling
Automated barcode reader to eliminate tedious rack
configuration.

11

Higher sample capacity
High sample capacity is standard (four plates versus
two plates on industry average). Increase capacity
further with the optional Thermo Scientific™ Vanquish™
Charger module.

12

Versatile column management
Greater column capacity (two 30 cm columns
or a greater number of shorter columns percompartment), plus class-leading temperature range
and temperature stability.

Accurate flow for more data confidence
The flow is delivered by a dual pump offering two
truly individual devices in a single housing with ternary
gradient proportioning.

13

Delivering powerful separations
Run samples with two similar or different columns
to increase throughput or maximize output. Setup
with optional second compartment for independent
column temperatures.

Simple maintenance
Module maintenance is reduced to a minimum andcan be worked on without instrument de-stacking,plus smart light bars on the front of the systemprovide instant system status update.

14

Keep the lab tidy
The built-in drawer stores accessories, related
documents, or any other useful material for the system.

Vanquish Solvent Monitor
Use the Vanquish Solvent Monitor to increase yourregulatory compliance, productivity, and safety ofyour laboratory. That device guarantees that your
HPLC systems will never run dry and the waste will
not spill over during your analysis, reducing loss ofvaluable measurement time and sample material.
Vanquish User Interface
With the Vanquish User Interface, you are the masterof your LC instrument. The display always shows you
the key parameters of your instrument such as flow
and pressure and guides you in a visual step-by-step
video tutorial through key maintenance tasks.
Versatile detection capabilities
Differing molecular characteristics of your analyte,
from small molecules to complex biomolecules,
necessitate multiple detector methodologies.
Vanquish Duo system for Dual LC can be used withany two detectors out of the broad detector portfolio
including MS, DAD, VWD, FLD and CAD.
Compatible with your software infrastructure
A Vanquish Duo UHPLC system for Dual LC can be
operated in various software environments including
the latest Chromeleon software feature release,
Chromeleon CDS long-term, and extended supportversion and Waters Empower.

1

2
3

4

3

5

5

6

6

7

7

8

10

11

9

12
13

14

9

Vanquish Duo System for Dual LC-MS
During gradient elution of LC-MS analysis, the elution
window of the compounds of interest is often only afraction of the total run time limiting the final sample
throughput. The Vanquish Duo system for Dual LC-MS
Single LC-MS
Channel LC throughput with a multi-channel
can double
Analytical Gradient
RecondiAnalytical Gradient
RecondiApplication
Flow
Path
1
LC approach
that1 eliminates
wastedfromApplication
tioning
Applicationtime
1
tioning the Switch Timeapplication.
Tandem LC or LC-MS
Flow Path 1
Flow Path 2
Flow Path 1

Analytical GradientApplication 1

SingleChannel LC
Recondi-tioning
Analytical GradientApplication 1

Analytical GradientApplication 1
Reconditioning
Reconditioning

Application
Switch Time

Analytical GradientApplication 1

Analytical GradientApplication 2

Reconditioning

Reconditioning
Application
Switch Time

• Double the throughput without compromisingdata quality• Small footprint for optimal bench space utilization• Improves flexibility by running two different methods
Analytical Gradient
RecondiAnalytical Gradient
RecondiApplication 2
tioning
Application 2
tioningsimultaneously• Accelerates return on investment with greater
MS utilization
Analytical GradientApplication 2

Reconditioning
Analytical GradientApplication 2

Time savings
Compared to single channel LC
Reconditioning

Analytical GradientApplication 2

Reconditioning

Dual LC-MS
Flow Path 1
Flow Path 1
Flow Path 2
Flow Path 2
Resulting
MS Signal

TandemLC Gradientor LC-MS RecondiAnalytical

Analytical Gradient
Reconditioning
Application 1
Application 1
tioning
Analytical Gradient
Analytical GradientApplication
Analytical GradientApplication 1 Analytical
Application
Time Gradient Application
Gradient 1 Recondi- Switch
Analytical
Recondi- 2
Application 2
Application 2
tioning
tioning
RecondiRecondiReconditioning
tioning
tioning
Application 1

Application 2

Application 1

Application 2

Reconditioning

Analytical GradientApplication 1

Reconditioning

Analytical GradientApplication 2
Reconditioning

Time savings
Compared to single channel LC

Time savings

Compared to single channel LC

Dual LC-MS
Flow Path 1

Analytical Gradient1
DualApplication
LC

Flow
Flow Path
Path 21

Analytical
Gradient
Analytical
Gradient
RecondiAnalytical Gradient
RecondiAnalytical
Gradient
RecondiApplication
Application
tioning 1
Application 1
tioning 2
Application
tioning 2

Flow Path 2
ResultingAnalytical GradientApplication 2

MS Signal

Application 1

Reconditioning
Application 2

Analytical GradientApplication 2
Application 1

Reconditioning

Time savingsTimesavings
Compared
to single
channel LC

Reconditioning

Compared to single channel LC

Application 2

Inverse Gradient LC — Improved quantification with CAD
Flow Path 1 DualLC
Analytical
Gradient
Application 1

Flow Path 2
Flow Path 1

Analytical GradientApplication 1

Flow Path 2

Analytical GradientApplication 2

Reconditioning

Reconditioning

Analytical GradientApplication 1

Analytical GradientApplication 1

Reconditioning

Reconditioning

Reconditioning

Analytical GradientApplication 2

Application
Switch Time

Analytical GradientApplication 2

Reconditioning

Analytical GradientApplication 2

Reconditioning

Time savings
Compared to single channel LC

Reconditioning

Inverse Gradient LC — Improved quantification with CAD
Flow Path 1
Flow Path 2

Analytical GradientApplication 1

Reconditioning

Analytical GradientApplication 1

Reconditioning

Application
Switch Time

Analytical GradientApplication 2

Reconditioning

Analytical GradientApplication 2

Dual LC
Flow Path 1

Analytical GradientApplication 1

Reconditioning

Analytical GradientApplication 1

Reconditioning

Flow Path 2

Analytical GradientApplication 1

Reconditioning

Analytical GradientApplication 1

Reconditioning

Dual LC
Flow Path 1

Analytical GradientApplication 1

Reconditioning

Analytical GradientApplication 1

Reconditioning

Flow Path 2

Analytical GradientApplication 1

Reconditioning

Analytical GradientApplication 1

Reconditioning

A Vanquish Duo system for Dual LC-MS is a multichannel LC with no
extra bench space required while offering doubled throughput with
Vanquish system inherent values of robustness and ease of use.

10

Reconditioning

The Dual LC-MS workflow consists of two
independent LC channels which can independently
operate to run the separation. This combination,
along with the smart scheduling capability of Thermo
Scientific Aria MX software, results in doubled
throughput without any sacrifice in data quality,
sensitivity or additional bench space.

Flow path1

Method 1
0 min

5 min

Flow path2

Method 2
0 min

7 min

Collected
MS data
0 min

Interlacing of two LC flow paths into one single mass spectrometerfor doubled throughput even with two different types of analysis.

Intelligent schedulingAria MX software manages and controls
all aspects of the Vanquish system. It is
powerful enough to schedule and manage
multiple methods on multiple channels
simultaneously. At the same time, it features
an intuitive graphical method editing,allowing anyone to quickly develop methods
and run batches of samples. Aria software
can be either used with Thermo Scientificmass spectrometer software such as
Thermo Scientific™ TraceFinder™ and
Xcalibur software and additionally integrates
into Analyst® software.

11

Vanquish Duo System for Tandem LC or LC-MS• Increasing sample throughput without modifying avalidated method• Improving return on investment by maximizing the
instrument utilization

Throughput increase by Tandem LC or LC-MS
90

Throughput increase (%)

Gradient separations are commonly used in LC or LC-MS.
Column reconditioning, such as washing andre-equilibration, takes time and reduces your overall sample
throughput. The Vanquish Duo system for Tandem LC or
LC-MS eliminates these limitations and improves
productivity by:■ 5 min Gradient■ 15 min Gradient0• Reducing column carry over through extended column
washing without sacrificing throughput■ 30 min Gradient50

2.1 × 50 mm

2.1 × 150 mm

Throughput increase by Tandem LC or LC-MS is between 10–80%
for most common conditions.

• Simplifying method setup with automatic method
conversion from your original gradient method
Single Channel LC
ChannelLC
Analytical
Gradient
Flow Path 1 Single
Flow Path 1

Reconditioning
Reconditioning

Application 1
Analytical GradientApplication 1

Analytical GradientApplication 1
Analytical GradientApplication 1

Reconditioning
Reconditioning

Application
Switch Time
Application
Switch Time

Analytical GradientApplication 2
Analytical GradientApplication 2

Reconditioning
Reconditioning

Analytical GradientApplication 2
Analytical GradientApplication 2

Reconditioning
Reconditioning

Tandem LC or LC-MS
LCGradientor LC-MS Analytical Gradient
Analytical
Flow Path 1 Tandem
Flow Path 1
Flow Path 2
Flow Path 2

Application 1
Analytical GradientApplication 1
Reconditioning
Reconditioning

Application 1
Analytical GradientApplication 1
Reconditioning
Reconditioning

Application
Switch Time
Application
Switch Time

Analytical GradientApplication 2
Analytical GradientApplication 2
Reconditioning
Reconditioning

Analytical GradientApplication 2
Analytical GradientApplication 2
Reconditioning
Reconditioning

Time savingsTimesavings
Compared
to single
channel LC
Compared to single channel LC

Dual LC-MS
Flow Path 1
Flow Path 1
Flow Path 2
Flow Path 2
ResultingMS
SignalResulting
MS Signal

Analytical
Dual
LC-MSGradient

RecondiAnalytical Gradient
Reconditioning
Application 1
Application 1
tioning
RecondiAnalytical Gradient
Analytical Gradient
Reconditioning
Gradient Application
Gradient
Recondi- 1 Analytical
Application 1 Analyticaltioning
Application 2
Application 2
tioning
Analytical Gradient
Analytical Gradient
RecondiApplication 2
Application 2
tioning

Reconditioning
Reconditioning

Time savings

Application 1

Application 2

Application 1

Application 2

Timesavings
Compared
to single
channel LC

Application 1

Application 2

Application 1

Application 2

Compared to single channel LC

Dual LC
Flow Path 1
Flow Path 1
Flow Path 2
Flow Path 2

Dual
LC
Analytical
Gradient

Application 1
Analytical Gradient
Analytical
Gradient
Application
1
Application 2
Analytical GradientApplication 2

Recondi- Analytical Gradient Reconditioning
Application 1
tioning
Recondi- Analytical Gradient RecondiRecondi- Application
Analytical1Gradienttioning
tioningReconditioning
Application 2
tioning
RecondiAnalytical Gradient
Reconditioning
Application 2
tioningTime savingsTimesavings
Compared
to single
channel LC
Compared to single channel LC

Inverse Gradient LC — Improved quantification with CAD
Flow Path 1 Inverse
Gradient
LC —RecondiImproved quantification
with CAD
Analytical
Gradient
Analytical Gradient
RecondiFlow
Flow Path
Path 2
1

Application 1
Analytical GradientApplication 1

tioning
Reconditioning

Application 1
Analytical GradientApplication 1

tioning
Reconditioning

Application
Switch Time
Application
Switch Time

Analytical GradientApplication 2
Analytical GradientApplication 2

Reconditioning
Reconditioning

Analytical GradientApplication 2
Analytical GradientApplication 2“Since installing the Tandem LC-MS system we have doubled
our throughput and increased the precision of our data!”
Flow Path 2

	—Dr. Richard Rogers, Just Biotherapeutics, Inc.

Dual LCLC
Analytical
Gradient
12Flow Path 1 Dual
Flow Path 1
Flow Path 2

Application 1
Analytical GradientApplication
1
Analytical
Gradient
Application 1
Analytical Gradient

Reconditioning
Reconditioning
Reconditioning
Recondi-Analytical GradientApplication 1
Analytical GradientApplication
1
Analytical
Gradient
Application 1
Analytical Gradient

Reconditioning
Reconditioning
Reconditioning
Recondi-Reconditioning
Reconditioning

1.6

Column A
cou

Thermo Scientific™ Acclaim™
VANQUISH™ C18

0

Column B
Thermo Scientific Acclaim
VANQUISH C18

cou

The Vanquish Duo system for Tandem
LC or LC-MS splits the analysis
between two pumps and twocolumns. One pump delivers theanalytical gradient while the second
pump runs the reconditioning
gradient. This configuration allows
you to use two identical columns to
run your sequence faster withoutcompromising data quality.

24.16
5

10

15

min

20

25

30

35

Reproducible results for the Thermo Scientific™ Vanquish™ Horizon Duo UHPLC system for
Tandem LC or LC-MS—Thermo Scientific™ Q Exactive™ HF hybrid quadrupole-Orbitrap™
mass spectrometer showing an overlay of five total ion current chromatograms. The
sample shown is digested Infliximab using the Thermo Scientific™ SMART™ Digest Kit.

“With the Vanquish Duo for Tandem LC-MS, we have two instruments
in one in terms of analysis time but it’s only occupying the footprint of asingle LC system. Of course it saves space, but furthermore it gives us
a more efficient utilization of our MS system.”—Dr. Dan Bach Kristensen, Principal Scientist, Symphogen

Simply upgrade your method
Implementing Tandem LC or LC-MS into yourworkflow is easy with compliance-ready
Chromeleon software for LC or LC-MS users
and Thermo Scientific Standard Instrument
Integration (SII) for Thermo Scientific Xcalibur
software for LC-MS users.
A simple dedicated instrument method wizard
enables you to create new methods without
the need for additional training.

Chromeleon CDS and SII for
Xcalibur guides users through theimplementation of a Tandem LC
or LC-MS method with automatic
system volume considerations
and gradient formation of thereconditioning pump.

13

Vanquish Duo System for Inverse Gradient
Vanquish Duo system for Inverse Gradient provides you
Single Channel LCa uniformAnalyticalresponse
forRecondiall detectable
analyteswith theGradient
Analytical Gradient
RecondiApplication
Flow Path 1
Application 1
tioning
Application 1
Switch Timesuperior charged
aerosoldetection(CAD). tioning
While isocratic
separations can yield a uniform CAD response, someseparations
gradient elution.
Tandemrequire
LC or LC-MS

By using an Inverse Gradient for compensation, theeluententering thedetector
always has
the same solvent
Analytical Gradient
RecondiAnalytical Gradient
RecondiApplication 2
Application 2
tioningcomposition,
no tioning
matter where
the compound
elutesduring the gradient. This gives you consistent responsefor quantitative information with high confidence even if
Analytical
Gradient
Analytical
Gradient
Application
Analytical
Gradient
Gradient
you Analyticallack anindividual standard.
Flow Path 1
Time savings
1
Application 1
Switch Time
Application 2
Application 2
By utilizing Applicationa secondpump
to deliver aninverse gradient,
RecondiRecondiRecondiCompared to single channel LC
Flow Path 2 Reconditioningoffers:
tioning
tioning
tioning
the detector• Uniform response with CAD under gradient elution
Dual LC-MS
conditions
Flow Path 1

Analytical GradientApplication 1

Reconditioning

Analytical GradientApplication 1

Reconditioning

Application 1

Application 2

Application 1

Application 2

Reconditioning

Analytical GradientApplication 1

Reconditioning•FlowReliable
standard-freeofknownsAnalytical quantification
Gradient
Analytical
Gradient andRecondiRecondiPath 2
Application 2
Application 2
tioning
tioning
unknowns
ResultingTime savings
Compared to single channel LC

Signal•MSSimplifying
method setup with automatic Inverse
Gradient calculation considering all system volumes
Dual LC
Flow Path 1
Flow Path 2
Flow Path 1

Analytical GradientApplication 1

SingleChannelLC
Analytical
Gradient
Application 2

Analytical GradientApplication 1

Reconditioning

Reconditioning

Analytical GradientApplication 2

Analytical GradientApplication 1

Time savings
Compared to single channel LC

Reconditioning

Reconditioning

Analytical GradientApplication 2

Application
Switch Time

Reconditioning

Analytical GradientApplication 2

Reconditioning

Reconditioning

Analytical GradientApplication 2

Reconditioning

Inverse Gradient LC — Improved quantification with CAD
LCGradientor LC-MS RecondiFlow Path 1 Tandem
Analytical
Flow Path 2
Flow Path 1

Application 1
Analytical GradientApplication 1

Analytical Gradient
Reconditioning
Application 1
tioning
Analytical GradientApplication
Application 1
Switch Time

Flow Path 2

Reconditioning

Reconditioning

Application
Switch Time
Analytical GradientApplication 2

Analytical GradientApplication 2
Analytical GradientApplication 2

Reconditioning

Reconditioning

Time savings

Compared to single channel LC

Dual LC-MS
RecondiAnalytical Gradient
Recondi-of unknown compounds
Quantification
recoverytioning
Application 1
tioning

Analytical GradientApplication 1

Flow Path 2 Dual LC
Flow Path 1
ResultingFlow
2
MS PathSignalAnalytical GradientApplication 1

Flow Path 2

Reconditioning

Reconditioning

UV

Analytical GradientApplication 2

Analytical GradientApplication 1

Reconditioning

Reconditioning

Charged Aerosol Detector

Analytical GradientApplication 1
Analytical GradientApplication 20

Recondi-low Analytical
GradienthighRecondimedium
tioning
tioning
organic Application
organic 1 organic

eluterReconditioning

eluter GradienteluterAnalytical
Application 2

loworganic
eluterReconditioning

Charged Aerosol Detector with
InverseGradientTimesavings

Compared to single channel LC

Application
1
Application
Application
1
Application
Analytical
Gradient
Recondi- 2 Analytical
Gradient
Recondi- 2
100
Application 1
tioning
Application 1
tioning

Dual LC
Flow Path 1

Analytical GradientApplication
2200

Quantification recovery (%)

Flow Path 1

mediumorganic
eluterhighorganic
eluterlow
organic
elutermediumhighelutereluterTimesavingsorganicorganic

Compared to single channel LC

For a gradient separation, the quantification recovery of unknown compounds with CAD isbest with the eluting conditions from the Inverse Gradient approach. For a standard CAD,
the quantification recovery will depend on the organic content. UV detection recovery
Analytical Gradient
RecondiAnalytical Gradient
RecondiApplication
Analytical Gradient
RecondiAnalytical Gradient
extinction
coefficient.
Application 1 depends
tioning on chromophore
Application 1
tioning
SwitchTime
Application 2
tioning
Application 2

Inverse Gradient LC — Improved quantification with CAD
Flow Path 1
Flow Path 2

14Flow Path 1
Flow Path 2

Dual LC
Analytical GradientApplication 1

Reconditioning

Analytical GradientApplication 1

Reconditioning

Analytical GradientApplication 1

Reconditioning

Analytical GradientApplication 1

Reconditioning

Reconditioning0.4

No extra work needed—
automatic gradient conversion● Adenine● Tenofovir● Emtricitabin● Tenofovir Disoproxil

Peak Area (pA*min)

0.3

0.2

0.1

0.0
0

10

20

30

40

50

40

50

Mass on Column (ng)0.4Chromeleon CDS automatically identifies the Inverse
Gradient instrument configuration and starts the
dedicated method wizard. The wizard calculates andapplies the Inverse Gradient to the second pump in
the Vanquish Duo system for Inverse Gradient taking
into account all internal volumes giving highest
quantification accuracy and maximized ease-of-use.

● Adenine● Tenofovir● Emtricitabin● Tenofovir Disoproxil

Peak Area (pA*min)

0.3

0.2

0.1

0.0
0

10

20

30

Mass on Column (ng)Calibration curves for four pharmaceuticals without(top) and with Inverse Gradient compensation (bottom)
showing similar response factors for all compounds.

See what you’ve been missing
Most detection options in liquid chromatographyrequire certain physico-chemical properties to detecta substance, such as a chromophore for UV lightabsorption or ionizability for mass spectrometry.
Charged aerosol detection quantifies any non-volatile
analyte independent of chemical structure while
simultaneously providing uniform response, excellent
sensitivity and a wide linear range.

Chromeleon CDS guides users through the implementation of
an Inverse Gradient method with automatic system volume
considerations and gradient formation.

15

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Thermo Scientific Vanquish Duo HPLC and UHPLC system workflows

Thermo Scientific
Vanquish Core HPLC system• Workhorse instrument todependably deliver results• System intelligence for
improved productivity• Seamless method transfer

Thermo Scientific
Vanquish Flex UHPLC
system
• Unparalleled flexibility andcontrol• High throughput, highsensitivity• Low pressure and highpressure solvent blending• Low pressure, high pressure,and isocratic solvent blending

700 barThermo Scientific
Vanquish Horizon UHPLC
system• Industry-leading precision
and accuracy in delivering
sample information• Unmatched detection,
sensitivity and speed• Truly future-proof withspecifications far in excess
of all others• Optimizes the benefits of
LC-MS analysis

1000 bar

The collective power of chromatography
LC that takes your productivity to new heights

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