English & Scientific Consulting Kft., Hősök tere 2, 3044 Szirák, Hungary

19/03/2014

Monoclonal Antibody J2
Description

J2 monoclonal antibody (mAb), mouse, IgG2a, kappa chain

Lot Nr.

J2-1303

Amount: 200 μg

J2-1303

Amount: 500 μg

Concentration
after reconstitution 1.00 mg/ml as determined as determined by A280 nm (A280 nm = 1.47
corresponds to 1 mg/ml antibody) gel electrophoretically pure IgG
antibody.
Reconstitution

The lyophilised sample should be reconstituted with:200 l sterile distilled water for 200µg antibody
500 l sterile distilled water for 500µg antibody.
The mAb will then be in PBS without any stabilisers or preservatives atthe concentration given.
As a result of the lyophilisation procedure, the reconstituted antibody
may contain small amounts of denatured protein in the form of
aggregates that may interfere with some applications such as
immunohistochemistry (e.g. by giving high backgrounds). We therefore
highly recommend centrifuging (microcentrifuge) the reconstituted
antibody before use and using the supernatant.

Specificity

The mAb J2 recognises double-stranded RNA (dsRNA) provided that thelength of the helix is greater than or equal to 40 bp. dsRNA-recognition
is independent of the sequence and nucleotide composition of theantigen. All naturally occurring dsRNAs investigated up to now (40-50species) as well as poly(I)poly(C) and poly(A)poly(U) have beenrecognised by J2, although in some assays its affinity to poly(I)poly(C)is about 10 times lower than that to other dsRNA antigens.

Applications

mAb J2 can be used for ELISA, dsRNA-immunoblotting,immunoaffinity chromatography and in certain systems also for
immunohistochemistry (see references).
Please note that nucleic acid separation prior to dsRNA-immunoblotting
must be carried out by polyacrylamide gel electrophoresis, because thesensitivity of detection is considerably lower after blotting from agarose
gels.
Not for use for clinical purposes. For in vitro use only.

Stability and storage
After reconstitution antibodies should be aliquoted and stored at -20 °C or -70°C.
After adding 10 mM sodium azide undiluted antibody can also be stored
at +4 °C for a short period of time. For long term storage the mAb should
be kept frozen. Repeated freezing/thawing cycles should be avoided.

English & Scientific Consulting Kft., Hősök tere 2, 3044 Szirák, Hungary

References

19/03/2014

Schönborn, J., Oberstrass, J., Breyel, E., Tittgen, J., Schumacher, J. andLukacs, N. (1991) Monoclonal antibodies to double-stranded RNA asprobes of RNA structure in crude nucleic acid extracts. Nucleic Acids
Res.19, 2993-3000.
Lukacs, N. (1994) Detection of virus infection in plants anddifferentiation between coexisting viruses by monoclonal antibodies todouble-stranded RNA. J. Virol. Methods 47, 255-272.
Lukacs, N. (1997) Detection of sense:antisense duplexes by structurespecific anti-RNA antibodies. In: Antisense Technology. A PracticalApproach, C. Lichtenstein amd W. Nellen (eds), pp. 281-295. IRL Press,Oxford.

When referring to this antibody in a publication, please cite „J2“ from “English and Scientific
Consulting, Hungary”. You may also include our weblink (http://www.scicons.eu).