RECOMMENDED READING

Publications with Dharmacon™
Edit-R™ CRISPR-Cas9 reagents

Introduction

8.

These publications demonstrate the application of CRISPR-Cas9 genome
engineering techniques for target gene knockout or precise knockin using
Dharmacon™ Edit-R™ CRISPR-Cas9 reagents or custom RNA synthesis.

9.

2021
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Bowling, E. A., Wang, J. H., Gong, F., et al (2021). Spliceosome-targeted
therapies trigger an antiviral immune response in triple-negative
breast cancer. Cell. 184(2), 384-403.e21. doi.org/10.1016/j.
cell.2020.12.031
Chabanon, R. M., Morel, D., Eychenne, T., et al (2021). Pbrm1 deficiency
confers synthetic lethality to dna repair inhibitors in cancer. Cancer
Research, 81(11), 2888–2902. doi.org/10.1158/0008-5472.CAN-21-0628
Dutta, R. K., Chinnapaiyan, S., Santiago, M. J., et al (2021).
Gene-specific MicroRNA antagonism protects against HIV Tat and
TGF-β-mediated suppression of CFTR mRNA and function.
Biomedicine & Pharmacotherapy, 142, 112090. doi.org/10.1016/j.
biopha.2021.112090
Gozgit, J. M., Vasbinder, M. M., Abo, R. P., et al (2021a). PARP7 negatively
regulates the type I interferon response in cancer cells and its inhibition
triggers antitumor immunity. Cancer Cell, 39(9), 1214-1226.e10.
doi.org/10.1016/j.ccell.2021.06.018
Jeusset, L. M., Guppy, B. J., Lichtensztejn, Z., et al (2021). Reduced
usp22 expression impairs mitotic removal of h2b monoubiquitination,
alters chromatin compaction and induces chromosome instability
that may promote oncogenesis. Cancers, 13(5), 1043. doi.org/10.3390/
cancers13051043
Jewett, C. E., Soh, A. W. J., Lin, C. H., et al (2021). Rab19 directs
cortical remodeling and membrane growth for primaryciliogenesis. Developmental Cell, 56(3), 325-340.e8. doi.org/10.1016/j.
devcel.2020.12.003
Martin-Sancho, L., Lewinski, M. K., Pache, L., et al (2021). Functionallandscape of SARS-CoV-2 cellular restriction. Molecular Cell, 81(12),2656-2668.e8. doi.org/10.1016/j.molcel.2021.04.008

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Mills, C. A., Wang, X., Bhatt, D. P., et al (2021). Sirtuin 5 is regulated by
the scf cyclin f ubiquitin ligase and is involved in cell cycle control.
Molecular and Cellular Biology, 41(2). doi.org/10.1128/MCB.00269-20
Yin, X., Riva, L., Pu, Y., et al (2021). Mda5 governs the innate immune
response to sars-cov-2 in lung epithelial cells. Cell Reports, 34(2),108628. doi.org/10.1016/j.celrep.2020.108628

2020
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Dermit, M., Dodel, M., Lee, F. C. Y., et al (2020). Subcellularmrna localization regulates ribosome biogenesis in migrating
cells. Developmental Cell, 55(3), 298-313.e10. doi.org/10.1016/j.
devcel.2020.10.006
E. Kostaras, T. Kaserer, G. Lazaro, et al. A systematic molecular andpharmacologic evaluation of AKT inhibitors reveals new insight into
their biological activity. British Journal of Cancer. (2020).
Ishibashi, M., Takahashi, R., Tsubota, A., et al (2020). Slamf3-mediatedsignaling via erk pathway activation promotes aggressive phenotypic
behaviors in multiple myeloma. Molecular Cancer Research, 18(4),632–643. doi.org/10.1158/1541-7786.MCR-19-0391
Russell, R., Carnese, P. P., Hennings, T. G., et al (2020). Loss of thetranscription factor MAFB limits β-cell derivation from human PSCs.
Nature Communications, 11(1), 2742. doi.org/10.1038/s41467-02016550-9
Segura-Bayona, S., Villamor-Payà, M., Attolini, C. S.-O., et al (2020).
Tousled-like kinases suppress innate immune signaling triggered byalternative lengthening of telomeres. Cell Reports, 32(5), 107983. doi.
org/10.1016/j.celrep.2020.107983
Shimizu, T., Nagane, M., Suzuki, M., et al (2020). Tumor hypoxia
regulates ganglioside GM3 synthase, which contributes to oxidative
stress resistance in malignant melanoma. Biochimica et Biophysica
Acta (BBA) - General Subjects, 1864(12), 129723. doi.org/10.1016/j.
bbagen.2020.129723
Tomizawa, F., Jang, M.-K., Mashima, T., et al (2020). C-kit regulates
stability of cancer stemness in cd44-positive colorectal cancer
cells. Biochemical and Biophysical Research Communications, 527(4),1014–1020. doi.org/10.1016/j.bbrc.2020.05.024

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R.M. Garner, G. Skariah, A. Hadjitheodorou, et al. Neutrophil-like
HL-60 cells expressing only GFP-tagged b-actin exhibit nearly normal
motility. Cytoskeleton. (2020). doi: 10.1002/cm.21603
T. Venit, K. Semesta, S. Farrukh, et al. Nuclear myosin 1 activates p21
gene transcription in response to DNA damage through to DNA
damage through a chromatin-based mechanism. Communications
Biology. 3(115). (2020). doi: 10.1038/s42003-020-0836-1

2019
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D.A. Erkes, C.O. Field, C. Capparelli, et al. The next-generation BET
inhibitor, PLX51107, delays melanoma growth in a CD8-mediatedmanner. Pigment Cell Melanoma Research. 32:687-696. (2019).
doi:10.1111/pcmr.12788
P. Janus, A. Toma-Jonik, N. Vydra, et al. Pro-death signaling ofcytoprotective heat shock factor 1: upregulation of NOXA leading to
apoptosis in heat-sensitive cells. Cell Death & Differentiation. (2020).
doi: 10.1038/s41418-020-0501-8
H. Khan, A. Anshu, A. Prasad, et al. Metabolic rewiring in response tobiguanides is mediated by mROS/HIF-1α in malignant lymphocytes.
Cell Reports. 29, 3009-3018 (2019). doi: 10.1016/j.celrep.2019.11.007
S. Kim, A. Bolatkan, S. Kaneko, et al. Deregulation of the histonelysine-specific demethylase I is involved in human hepatocellular
carcinoma. Biomolecules. 9:810 (2019). doi: 10.3390/biom9120810
A.R. Leenay, A. Aghazadeh, J. Hiatt, et al. Large dataset enables
prediction of repair after CRISPR-Cas9 editing in primary T cells.
Nature Biotechnology. (2019). doi: 10.1038/s41587-019-0203-2
J. Li, R. An, S. Lai, et al. Dysregulation of PP2A-Akt interaction
contributes to sucrose non-fermenting related kinase (SNRK)
deficiency induced insulin resistance in adipose tissue. Molecular
Metabolism. (2019). doi: 10.1016/j.molmet.2019.07.009
J. Iwasaki, T. Komori, F. Nakagawa, et al. Schlafen11 expression isassociated with the antitumor activity of trabectedin in humansarcoma cell lines. Anticancer Research. 39:3553-3563 (2019). doi:
10.21873/anticanres.13501
A. Shariati, A. Dominguez, et al. Reversible Disruption of Specific
Transcription Factor-DNA Interactions using CRISPR/Cas9. Mol Cell.
74(3), 622-633.e4 (2019). doi: 10.1016/j.molcel.2019.04.011
S. Weigle, E. Martin, et al. Primary cell-based phenotypic assays topharmacologically and genetically study fibrotic diseases in vitro.
J Biol Methods. 6(2):e115 (2019). doi: 10.14440/jbm.2019.285
A. Perota, I. Lagutina, et al. Generation of Cattle Knockout forgalactose-a1,3-galactose and N-glycolylneuraminic acid antigens.
Xenotransplantation. 00:e12524 (2019). doi: 10.1111/xen.12524
D.A. Erkes, C.O. Field, et al. The next-generation BET inhibitor,
PLX51107, delays melanoma growth in a CD8-mediated manner.
Pigment Cell Melanoma Res. 00, 1-10 (2019). doi: 10.1111/pcmr.12788
S. Kobayashi, T. Contractor, et al. Alleles of Insm1 Determine Whether
RIP1-Tag2 Mice Produce Insulinomas or Nonfunctioning Pancreatic
Neuroendocrine Tumors. Oncogenesis. 8:16, 1-13 (2019). doi: 10.1038/
s41389-019-0127-1
I. Rezuchova, S. Hudecova, et al. Type 3 Inositol 1,4,5-triphosphate
Receptor has Antiapoptotic and Proliferative Role in Cancer Cells.
Cell Death & Disease. 10:186, 1-10 (2019). doi: 10.1038/s41419-019-1433-4
W. Zhang, J. Wells, et al. miR-147b-mediated TCA cycle dysfunction
and Pseudohypoxia Initiate Drug Tolerance to EGFR Inhibitors inLung Adenocarcinoma. Nature Metabolism. (2019). doi: 10.1038/
s42255-019-0052-9
F. Fekri, J. Abousawan, et al. Targeted enhancement of flotillindependent endocytosis augments cellular uptake and impact ofcytotoxic drugs. BioRxiv. doi: 10.1038/s41598-019-54062-9

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P. Palmbos, Y. Wang, et al. ATDC Mediates a TP63-regulated Basal
Cancer Invasive Program. Oncogene. 38, 3340-3354 (2019). doi:
10.1038/s41388-018-0646-9
C. Buffone, J. Kutzner, et al. The Ability of SAMHD1 to block HIV-1 but
not SIV requires expression of MxB. Virology. 531, 260-268 (2019). doi:
10.1016/j.virol.2019.03.018

2018
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K. Hinohara, H-J. Wu, et al. KDM5 Histone Demethylase Activity Links
Cellular Transcriptomic Heterogeneity to Therapeutic Resistance.
Cancer Cell. 34, 1-15 (2018). doi: 10.1016/j.ccell.2018.10.014
E.M. Anderson, S. McClelland, E. Maksimova, et al. Lactobacillus
gasseri CRISPR-Cas9 characterization In Vitro reveals a flexible modeof protospacer-adjacent motif recognition. PLoS One. 13(2): e0192181(2018). doi: 10.1371/journal.prone.0192181
T. Araujo, A. Khayat, L. Quintana, et al. Piwi like RNA-mediated genesilencing 1 gene as a possible major player in gastric cancer. World J
Gastroenterol 24(47):5338-5350 (2018). doi: 10.3748/wjg.v24.i47.5338
A. Cluse, I. Nikolic, et al. A Comprehensive Protocol Resource for
Performing Pooled shRNA and CRISPR Screens. Methods Mol Biol.
1725, 201–227 (2018). doi: 10.1007/978-1-4939-7568-6_17
K. S. Kim, N. Maio, et al. Cytosolic HSC20 integrates de novo iron-sulfur
cluster biogenesis with the CIAO1-mediated transfer to recipients.
Hum Mol Genet. 27, 827–852 (2018). doi: 10.1093/hmg/ddy004
S. Melnik, D. Dvornikov, et al. Cancer cell specific inhibition of Wnt/
β-catenin signaling by forced intracellular acidification. Cell Discov.
4, 37 (2018). doi: 10.1038/s41421-018-0033-2
S. J. Pettitt, D. B. Krastev, et al. Genome-wide and high-density
CRISPR-Cas9 screens identify point mutations in PARP1 causing
PARP inhibitor resistance. Nat Commun. 9, 1849 (2018). doi: 10.1038/
s41467-018-03917-2
P. G. Ziros, I. G. Habeos, et al. NFE2-Related Transcription Factor 2
Coordinates Antioxidant Defense with Thyroglobulin Production
and Iodination in the Thyroid Gland. Thyroid. 28, 780–798 (2018).
doi: 10.1089/thy.2018.0018

2017
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M. Basila, M. L. Kelley, et al. Minimal 2’-O-methyl phosphorothioate
linkage modification pattern of synthetic guide RNAs for increased
stability and efficient CRISPR-Cas9 gene editing avoiding cellular
toxicity. PLoS One. 12, e0188593 (2017). doi: 10.1371/journal.
pone.0188593
C. E. Delaney, A. T. Chen, et al. A histone H4 lysine 20 methyltransferase
couples environmental cues to sensory neuron control ofdevelopmental plasticity. Development. 144, 1273–1282 (2017).
doi: 10.1242/dev.145722
S. S. Gang, M. L. Castelletto, et al. Targeted mutagenesis in a humanparasitic nematode. PLoS Pathog. 13, e1006675 (2017). doi:10.1371/
journal.ppat.1006675
J. F. Hultquist, J. Hiatt, et al. A CRISPR-Cas9 Genome Engineering
Platform in Primary CD4+ T Cells for the Interrogation of HIV Host
Factors. bioRxiv 205500 (2017) doi: 10.1101/205500
S. Kim, S. R. F. Twigg, et al. Localized TWIST1 and TWIST2 basic domain
substitutions cause four distinct human diseases that can be modeledin Caenorhabditis elegans. Hum Mol Genet. 26, 2118–2132 (2017). doi:
10.1093/hmg/ddx107
N. Maio, K. S. Kim, et al. A Single Adaptable Cochaperone-Scaffold
Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian
Respiratory Chain Complexes I-III. Cell Metab. 25, 945-953, e6 (2017).
doi: 10.1016/j.cmet.2017.03.010

A. Paix, A. Folkmann, et al. Precision genome editing using synthesisdependent repair of Cas9-induced DNA breaks. Proc Natl Acad Sci U S A.
114, E10745-E10754 (2017). doi: 10.1073/pnas.1711979114
8. A. Paix, A. Folkmann, et al. Precision genome editing using CRISPR-Cas9
and linear repair templates in C. elegans. Methods. 121–122, 86–93(2017). doi: 10.1016/j.ymeth.2017.03.023
9. L. J. Rupp, K. Schumann, et al. CRISPR/Cas9-mediated PD-1 disruption
enhances anti-tumor efficacy of human chimeric antigen receptor
T cells. Sci Rep. 7, 737 (2017). doi:10.1038/s41598-017-00462-8
10. Ž. Strezoska, M. Perkett, et al. High-content analysis screening for cell
cycle regulators using arrayed synthetic crRNA libraries. J. Biotechnol.
251, 189-200 (2017). doi: 10.1016/j.jbiotec.2017.04.017
11. X. M. van Wijk, S. Döhrmann, et al. Whole-Genome Sequencing ofInvasion-Resistant Cells Identifies Laminin α2 as a Host Factor for
Bacterial Invasion. MBio. 8, e02128-16 (2017). doi: 10.1128/mBio.02128-16
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2015
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2016
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T. A. Aguilera, M. Rafat, et al. Reprogramming the immunologicalmicroenvironment through radiation and targeting Axl. Nat Commun. 7,13898 (2016). doi: 10.1038/ncomms13898
R. Eggenschwiler, M. Moslem, et al. Improved bi-allelic modification of atranscriptionally silent locus in patient-derived iPSC by Cas9 nickase. Sci
Rep. 6, 38198 (2016). doi: 10.1038/srep38198
K. He, E. Chou, et al. Conjugation and evaluation of triazole-linked single
guide RNA for CRISPR-Cas9 gene editing. ChemBioChem. 17, 1809–1812(2016). doi:10.1002/cbic.201600320
J. F. Hultquist, K. Schumann, et al. A Cas9 Ribonucleoprotein Platform
for Functional Genetic Studies of HIV-Host Interactions in Primary
Human T Cells. Cell Reports 17, 1438–1452 (2016). doi:/10.1016/j.
celrep.2016.09.080
M. L. Kelley, Ž. Strezoska, et al. Versatility of chemically synthesized
guide RNAs for CRISPR-Cas9 genome editing. J. Biotechnol. 233, 74–83(2016). doi:10.1016/j.jbiotec.2016.06.011
J. McCaﬀrey, J. Sibert, et al. CRISPR-CAS9 D10A nickase target-speciﬁc
ﬂuorescent labeling of double strand DNA for whole genome mappingand structural variation analysis. Nucleic Acids Res. 44, e11 (2016).
doi:10.1093/nar/gkv878
V. Müller, F. Rajer, et al. Direct identification of antibiotic resistancegenes on single plasmid molecules using CRISPR/Cas9 in combination
with optical DNA mapping. Sci Rep. 6, 37938 (2016).
doi:10.1038/srep37938
A. Paix, H. Schmidt, et al. Cas9-assisted recombineering in C. elegans:genome editing using in vivo assembly of linear DNAs. Nucleic Acids Res.
44, e128 (2016). doi:10.1093/nar/gkw502
J. Tan, S. E. Martin. Validation of Synthetic CRISPR Reagents as a Tool for
Arrayed Functional Genomic Screening. PLoS One 11, e0168968 (2016).
doi: 10.1371/journal.pone.0168968

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E. M. Anderson, A. Haupt, et al. Systematic analysis of CRISPR-Cas9
mismatch tolerance reveals low levels of off-target activity. J. Biotechnol.
211, 56-65 (2015). doi:10.1016/j.jbiotec.2015.06.427
R. Barrangou, A. Birmingham, et al. Advances in CRISPR-Cas9 genome
engineering: lessons learned from RNA interference. Nucleic Acids Res. 43,
3407–3419 (2015). doi:10.1093/nar/gkv226
H. Ogiwara, M. Sasaki, et al. Targeting p300 addiction in CBP-deficient
cancers causes synthetic lethality by apoptotic cell death due toabrogation of MYC expression. Cancer Discov. 6, 430–445 (2015).
doi:10.1158/2159-8290.CD-15-0754
S. Opp, D. A. S. A. Vieira, et al. MxB Is Not Responsible for the Blocking of
HIV-1 Infection Observed in Alpha Interferon-Treated Cells. J. Virol.
90, 3056-3064 (2015). doi:10.1128/JVI.03146-15
A. Paix, A. Folkmann, et al. High efficiency, homology-directed genome
editing in Caenorhabditis elegans using CRISPR-Cas9 Ribonucleoprotein
complexes. Genetics. 201, 47–54 (2015)
doi:10.1534/genetics.115.179382
G. Sivan, P. Ormanoglu, et al. Identification of Restriction Factors by
Human Genome-Wide RNA Interference Screening of Viral Host Range
Mutants Exemplified by Discovery of SAMD9 and WDR6 as Inhibitors of
the Vaccinia Virus K1L-C7L- Mutant. MBio. 6, e01122 (2015). doi:10.1128/
mBio.01122-15
W. Deng, X. Shi, et al. CASFISH: CRISPR/Cas9-mediated in situ labelingof genomic loci in fixed cells. Proc Natl Acad Sci U S A. 112, 11870–11875(2015). doi:10.1073/pnas.1515692112

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