gentleMACS™ Octo Dissociatorwith Heaters
User manualCopyright © 2022 Miltenyi Biotec and/or its affiliates. All rights reserved.
No part of this user manual may be reproduced, stored in a retrieval system, transmitted, published, or distributed in any form or by any means,electronically, mechanically, by photocopying, microfilming, recording, or otherwise, without the prior written consent of Miltenyi Biotec; however,notwithstanding the foregoing, the owners of the gentleMACS Octo Dissociator with Heaters may make copies solely for purposes of training
personnel in the use and servicing of the unit within their business or organization. Maximal care has been taken by Miltenyi Biotec in the preparationof this user manual. However, Miltenyi Biotec shall not be liable for any technical or editorial errors or omissions contained herein, or for incidental orconsequential damages in connection with the furnishing, performance, or use of this document. The information in this document is provided “as is”
without warranty of any kind, and is subject to change without notice. Unless otherwise specifically indicated, Miltenyi Biotec products and services are
for research use only and not for therapeutic or diagnostic use. gentleMACS, MACS, and the Miltenyi Biotec logo are either registered trademarks ortrademarks of Miltenyi Biotec and/or its affiliates in various countries worldwide.

gentleMACS™ Octo Dissociator with
Heaters
User manual
Original instructions
Issued: 2022-10
140-004-386.06

Miltenyi Biotec B.V. & Co. KG
Friedrich-Ebert-Straße 68
51429 Bergisch Gladbach
Germany
Phone +49 2204 8306-0
Fax +49 2204 85197
macsde@miltenyi.com
www.miltenyibiotec.com

Read the user manual before using the instrument
Before using the instrument, read chapter Important safety information and all other information containedin this user manual, including all safety and operating instructions. Pay special attention to warnings displayedon the instrument. Failure to read and follow these guidelines could lead to improper or incorrect usage, andresult in damage to the instrument. Improper usage could also cause severe personal injury, death,unpredictable results, instrument malfunction, and premature wear of components shortening the lifetime of
the instrument. Such actions may void your warranty. Keep this user manual and any other safety andoperating instructions provided with the instrument in a safe place that is accessible to all users for reference.
If you are concerned about the safe use of the instrument, please contact your authorized Miltenyi Biotec
service provider or call Miltenyi Biotec Technical Support.

2

Content
Important safety information

5

Wichtige Sicherheitsinformationen

13

Información importante de seguridad

21

Informations de sécurité importantes

29

Importanti istruzioni di sicurezza

37

1 Introduction

45

1.1 Preparation of single-cell suspensions and homogenates from tissues

45

1.2 Sample preparation for cell and molecular biology applications45

1.3 Sample preparation combining enzymatic and mechanical dissociation

46

1.4 MACS Tissue Dissociation Kits

47

1.5 Pre-defined tissue dissociation and homogenization programs47

1.6 Customized dissociation programs47

1.7 Intended use

47

2 Installation

49

2.1 Components included in the delivery49

2.2 Installation

49

3 Materials required51

3.1 Materials required to operate the instrument51

3.2 Spare parts

51

4 Instrument operation

53

4.1 General information for operating the instrument53

4.2 Instrument schematics

53

4.3 Starting the Instrument54

4.3.1 Menu tabs

4.4 Main menu

56

57

4.4.1 Status of sample tube positions

58

4.4.2 Processing samples

59

3

4.5 Editor menu
4.5.1 Creating a new program

65

4.5.2 Editing programs

71

4.5.3 Creating program shortcuts into Favorites

72

4.5.4 Transfering programs to other folders

72

4.5.5 Deleting programs

74

4.6 Setup

4

65

74

4.6.1 Set LED

75

4.6.2 Set Clock and date75

4.6.3 Set folder name75

4.6.4 Software version

75

4.6.5 Serial number

75

5 Maintenance77

5.1 Servicing

77

5.2 Cleaning77

5.3 Instrument disposal

78

6 Troubleshooting

79

7 Technical specifications81

8 Technical support

85

9 Legal notes

87

9.1 Limited warranty

87

9.2 Trademarks

88

IMPORTANT SAFETY INFORMATION

EN
Important safety informationEN

The gentleMACS Octo Dissociator with Heaters is a laboratory instrument. The instrument can be used safely, if
the following is ensured: The instrument is installed correctly. The instrument is only operated by professionalDElaboratory personnel. General safety practices are followed. All instructions in this user manual are followed.

Warnings and precautions

ES

The guidelines in this section explain the potential risk associated with operating this instrument and provide
important supplemental safety information to minimize the risks. Follow the instructions carefully to protect
yourself, others, and the equipment from potential hazards and create a safe work environment. Use thisFR

instrument only as specified by the manufacturer to avoid damage of equipment and injury to personnel.
Always follow local working area safety instructions and laboratory policies as well as standards for health,ITsafety and prevention of accidents. Contact the local authorities governing electrical power supply, building
constructions, maintenance, or safety for more information about the safe installation and operation of the
instrument.

Hazard levels
Signal words are used to identify safety and property damage messages. The following signal words are used
to throughout this user manual.
or WARNING! indicates a potentially hazardous situation, which, if not avoided, could result in death or serious
injury.
or CAUTION! indicates a potentially hazardous situation, which, if not avoided, may result in minor ormoderate injury. It may also be used to alert against unsafe practices.

5

IMPORTANT SAFETY INFORMATION

The following symbols are used to highlight conditions that could cause injury to personnel.

EN

Symbols

Risk of unmeant trapping of finger by hazardous rotating part.

Safety alert. Risk of danger. The documentation needs to be consulted in all cases where this safety alert
symbol is used on the instrument, in order to find out the nature of the potential hazard and any actions thathave to be taken.
Biohazard. Risk of contamination if potentially dangerous biological material is used.
Risk of electric shock.
Hazardous optical radiation.

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Hazardous hot surface.
Noise hazard.

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Protective conductor terminal. Symbol is attached on the inside of the instrument. Information for service
personnel.

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Fuse.
WEEE (Waste of Electrical and Electronic Equipment).

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On (Power supply).
Off (Power supply).
All operating instructions must be read before installing and operating the instrument.

European Conformity marking
United Kingdom Conformity Assessed marking
NRTL certification mark: Product meets consensus-based standards of safety, required by the Occupational
Safety/Health Administration (OSHA), determined by the Nationally Recognized Testing Laboratories (NRTL)TÜV SÜD

Safety label
The following safety label is attached to the back of the instrument and plays an important part in maintaining
safety.
WARNING! Avoid serious injury. Read and understand operating instructions
before installing or use. If manual is missing, obtain replacement from
Miltenyi Biotec.

6

DE

EN

IMPORTANT SAFETY INFORMATIONThe Trough is marked with the following safety symbol.

Figure 1: Safety symbol on the Trough of the gentleMACS Octo Dissociator with Heaters. The Sleeves are marked with a contrasting

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safety print.

Figure 2: Safety symbol on the gentleMACS Heaters.

The Sleeves are marked with a contrasting safety print. The contrasting safety print allows the visual perceptionof the rotation of the Sleeves.
The mentioned above safety labels and safety markings must be kept clean and legible. Periodically inspect thesafety labels and safety markings and replace them if they are not legible or perceptible from a safe viewing
distance. Contact Miltenyi Biotec for replacement labels.

General safety instructions
Safe operation of the instrument is no longer ensured if the instrument is not working properly and/or the
displayed instructions or messages advise you to contact Technical Support. Immediately switch off andunplug the instrument from the power outlet and contact an authorized Miltenyi Biotec service provider or
Miltenyi Biotec Technical Support.

Electricity and fire hazards
Potential risks arising from electrical devices include electric shock, short, and overheating. Electric
shock may lead to severe personal injury or even death. An electric short or overheating may cause a7

fire. Electronic equipment might emit sparks, which could ignite combustible vapors or material resulting in
IMPORTANT SAFETY INFORMATION

explosion or fire. Do not use the instrument in areas classified as hazardous locations; for example, in oxygenladen environments.
The housing of the instrument is designed to reduce the risk of electric shock, electric short, and spread of fire.
Except for the Safety Shield and Trough, do not remove or penetrate any cover. Only authorized personnel
must remove the other covers of the instrument. Never push a foreign object through an opening into theDEEN

instrument. Do not use the instrument ifl

it is opened or disassembled,lit has been dropped or damaged,lit has damaged or broken parts,lit has a damaged power cable,lan object has entered through the ventilation slots,la foreign object has been dropped into the instrument.

If flames or smoke appear, immediately switch off the power supply, unplug the instrument from the electricalES

outlet, and contact an authorized Miltenyi Biotec service provider or Miltenyi Biotec Technical Support.
The instrument is intended for indoor use only. If fluids enter the instrument, it may lead to electricFR

shorts, electric shock or fire. Take special care while handling fluids. Do not allow fluids to enter the interiorof the instrument.

IT

Protect the instrument against accidental spillages and splashes. Clean up spillages immediately. Do not
operate the instrument if liquids have spilled into the instrument. Do not use the instrument in a wet or damp
location. Avoid areas with high humidity or condensation. Moving the instrument from a cold environment –such as a cold room at 5 °C – to room temperature may cause condensation inside the instrument. In suchcases, wait for the instrument to dehumidify before operating it. Unplug the instrument from the electricaloutlet before cleaning.
Do not use liquid or aerosol cleaning agents; always use a damp cloth.
Ensure adequate air circulation in the room where the instrument is operated. In the absence of adequate
circulation, ambient air may not cool the instrument to acceptable operating temperatures. Allow sufficient air
circulation around the instrument – at least 15 cm on all sides – during operation to ensure adequate cooling.
Do not place the instrument near radiators, heat registers, stoves, or other equipment (including amplifiers)that generate heat. Protect the instrument from direct sunlight. Do not cover the slots and openings of the
instrument as these are provided for ventilation and protect the instrument from overheating. Do not place theinstrument in a built-in rack or similar confined spaces unless the space has been specifically designed toprovide proper ventilation. Follow the mounting instructions for the instrument.
Ensure that the mains switch as well as the connector for the power cable are easily accessible and located as
close to the operator of the instrument as possible. If it is necessary to disconnect the power supply, unplug
the cable from the power outlet.
The instrument is equipped with a three-wire electrical grounding-type plug that has a third pin for grounding.
This plug only fits into a grounded power outlet.
This is a safety feature. Do not try to insert the plug into a non-grounded power outlet. If you cannot insert theplug into the outlet, contact your local electrician to replace the outlet.

8

The instrument should only be operated from a power source that meets the specifications mentioned on the
IMPORTANT SAFETY INFORMATION

product’s electrical ratings label. If you have questions about the type of power source to use, contact yourauthorized Miltenyi Biotec service provider or local power company. Do not use extension cords or power
strips. Do not overload an electrical outlet.

Mechanical hazards
Moving and revolving parts are potential mechanical hazards. When operating the instrument, keep theTrough, Sleeves and Safety Shield attached to it. When the instrument's rotors are rotating, do not touch themor load or remove tubes from the instrument. Only use properly installed gentleMACS Tubes with tightly closed
caps. Do not operate the instrument with only gentleMACS Tubes caps in place.

EN

The exposed cutting edges of the gentleMACS Tubes caps may lead to incision wounds if touched while they
are rotating. Do not circumvent any safety measures or devices.

DE

Chemical and biological hazards
Depending on the biological material used, contamination infection may lead to severe personal injury or
death. All clinical samples must be considered potentially infectious.

ES

If biohazardous material is or has been used, the operator must choose and wear personal safety
equipment as indicated in the warnings and precautions for the particular substance. The above safety

FR

precaution also accounts for any hazardous chemical, including toxic or corrosive chemicals, acidic orradioactive substances that may be present in the sample. Wear protective gloves and clothing, as well as
safety glasses to prevent contact of the substance with skin and eyes. Defective or inadequate safetyITequipment is hazardous. The instrument must be operated in a safety hood if hazardous or unknown material
is being processed. If hazardous material has been used or spilled, care must be taken to thoroughly
decontaminate the instrument. It is strictly prohibited to continue to handle contaminated accessories or partsof the instrument.
Tubes and any other consumable that were in contact with biohazardous material must be decontaminatedbefore disposal. All liquid and solid waste must be considered hazardous and, must be therefore handled
taking universal laboratory precautions. Waste disposal must be in accordance with any local regulations.

Optical radiation hazards
The instrument is equipped with powerful light emitting diodes (LEDs) for illuminating the Safety Shield andthe gentleMACS Tubes. According to the international standard IEC 62471, this lamp system has an exposure
hazard value (EHV) of 0.91 and is in excess of the Exempt Risk Group. The hazard distance (HD) for the Exempt
Risk Group is 61 cm. The hazard distance for Risk Group 1 is 20 cm. The viewer-related risk is dependent on
how the instrument is installed and used. The optical radiation emitted from these LEDs may be harmful to theeyes at close viewing distances of the Trough is removed. Unplug the instrument before removing theThrough. If the Trough is removed, do not stare at the operating illumination LEDs from a distance lesser than
20 cm.
In addition, the integrated 2D code reader uses LEDs for illuminating the reading area. Do not stare directly atthe 2D code reader from a short distance while it is in operation.
Do not disassemble, modify, or remove any of the optical radiation sources. Optical radiation of disassembled
instruments may be harmful to the eyes.

9Hot surface hazards
IMPORTANT SAFETY INFORMATION

The heating foil of the gentleMACS Heaters may become hot enough to cause burns if touched. Do not touch
the heating foil, while the instrument is in operation.

Noise level hazards
If the instrument is used at maximum load, it might produce noise at a level, which could cause a hazard.
Hazardous noise levels may result in hearing impairment. It is recommended that the responsible bodymeasures or calculates the sound pressure level at the operator's position and at whatever point 1 m from theenclosure of the equipment has the highest sound pressure level. If it is not ensured during installation that thesound pressure level from the device will not reach a value at its point of use, that could cause a hazard, thenEN

protective materials such ad noise reducing baffles or hoods, ear protection equipment has to be used.
Observe local regulations regarding hearing protection.

DE

Servicing, transportation, and disposal
Unless otherwise specifically noted in this user manual or other Miltenyi Biotec documentation, do not serviceESthe instrument yourself. Servicing and repair must be performed by qualified service personnel. Improper orincorrect servicing or repair of the instrument can cause hazards to users, lead to unpredictable results, cause
instrument malfunction or damage, as well as premature wear and reduced life of the instrument. It may alsoFRvoid your warranty.
When replacement or spare parts are required, ensure that the service provider uses only genuine Miltenyi

IT

Biotec parts, or third-party parts specified and recommended by Miltenyi Biotec. Using unauthorized parts cancause malfunction of the instrument and impair results. Miltenyi Biotec does not honor any warranty or accept
any responsibility for instrument failure or damages resulting from the use of inappropriate parts. Aftercompleting any service or repair work, ensure that your authorized Miltenyi Biotec service provider performs all
safety checks as required by the repair procedure to ensure that the instrument is operating correctly.
Only use options and upgrades recommended by Miltenyi Biotec. Inquire with your local Miltenyi Biotec
representative about Miltenyi Biotec’s extensive instrument service and support arrangements, or refer towww.miltenyibiotec.com/support.
The instrument should be transported with care in packaging specified by Miltenyi Biotec. Internal damage canoccur if the instrument is subjected to excessive vibration or dropped. If the instrument needs to be shippedback to the manufacturer for service, decontaminate the instrument to remove any hazardous material prior to
shipment. If you have questions regarding proper decontamination or shipment, contact Miltenyi Biotec
Technical Support for assistance.

Waste of Electrical and Electronic Equipment (WEEE) customer information
Please dispose of your end-of-life Miltenyi Biotec products in accordance with the applicable WEEE andhazardous waste disposal legislation, which may differ by country or region.
Electrical and electronic equipment may contain hazardous substances which may have a serious detrimental
effect on the environment and/or human health. That is why all equipment must be specifically collected andtreated by designated waste facility centres and by qualified WEEE compliance schemes. By ensuring that you
dispose your unwanted electrical and electronic equipment according to the applicable WEEE and hazardous
waste disposal legislation, you are helping to preserve our natural resources and protect human health.

10Miltenyi Biotec is committed to protecting the environment. Miltenyi Biotec offers product end-of-life return
IMPORTANT SAFETY INFORMATION

programs in many countries, and partners with licensed WEEE compliance schemes throughout the world.
Miltenyi Biotec lets you recycle your end-of-life Miltenyi Biotec equipment free of charge. The terms andavailability of this offer vary by geography because of differences in regulatory requirements. Please note that,
depending on the type and use of your equipment, additional requirements may apply.
For more information, or if you wish to dispose of your end-of- life Miltenyi Biotec equipment, please contact

IT

FR

ES

DE

EN

your local Miltenyi Biotec representative or Miltenyi Biotec Technical Support.

11

12

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EN
IMPORTANT SAFETY INFORMATIONWICHTIGE SICHERHEITSINFORMATIONEN

DE
Wichtige SicherheitsinformationenEN

Der gentleMACS Octo Dissociator with Heaters ist ein Laborgerät. Das Gerät kann sicher verwendet werden,
wenn Folgendes sichergestellt ist: Das Gerät ist ordnungsgemäß installiert. Das Gerät wird nur vonprofessionellem Laborpersonal verwendet. Die allgemeinen Sicherheitsvorkehrungen werden befolgt.

DE

Sämtliche Anweisungen aus diesem Benutzerhandbuch werden befolgt.

ES

Warnungen und Sicherheitshinweise
Die in diesem Kapitel enthaltenen Sicherheitsrichtlinien erläutern die mit dem Betrieb dieses Gerätes
verbundenen möglichen Gefahren und geben wichtige zusätzliche Informationen zur Sicherheit, um diese

FR

Risiken zu minimieren.
Befolgen Sie diese Sicherheitshinweise sorgfältig um sich, andere und das Gerät vor möglichen Gefahren zu
schützen und ein sicheres Arbeitsumfeld zu schaffen.

IT

Um Sach- und Personenschäden zu vermeiden, benutzen Sie das Gerät ausschließlich nach Angaben desHerstellers.
Örtliche Arbeitsschutzbestimmungen, Laborrichtlinien, Sicherheitsnormen und Unfallverhütungsvorschriftenmüssen jederzeit beachtet und eingehalten werden. Wenden Sie sich für weitere Informationen zur
Stromversorgung, Gebäudeinstallationen, Wartung und Sicherheit für die Installation dieses Gerätes an dieörtlichen Behörden und Ihren Stromversorger.

Gefahrenstufen
Gefahrensymbole werden verwendet, um auf Sicherheits- und Sachschadenhinweise aufmerksam zu machen.
Folgende Gefahrensymbole werden in diesem Benutzerhandbuch benutzt.
oder WARNING! bezeichnet eine Gefahrensituation, die, falls sie nicht vermieden wird, zum Tode oderschwerwiegenden Verletzungen führen kann.
oder CAUTION! bezeichnet eine Gefahrensituation, die, falls sie nicht vermieden wird, zu leichten odermittelschweren Verletzungen führen kann. Es kann auch verwendet werden, um vor unsicherem Gebrauch zu
warnen.

SymboleDie folgenden Symbole werden benutzt um Gefahrensituationen anzuzeigen, welche zu Sach- und
Personenschäden führen können:
Warnzeichen. Gefahrenrisiko. Dieses Benutzerhandbuch muss immer konsultiert werden, wenn dieses
Warnzeichen benutzt wird, um mehr über die möglichen Gefahren und entsprechende Handlungsanweisungenzu erfahren.
Biologische Gefährdung. Risiko der Kontamination, wenn mit möglicherweise gefährlichen biologischen

13

WICHTIGE SICHERHEITSINFORMATIONEN

Substanzen gearbeitet wird.
Gefahr durch Stromschlag.
Gefahr durch optische Strahlung.
Gefahr unbeabsichtigten Einklemmens der Finger durch rotierende Bauteile.
Gefährdung durch heiße Oberfläche.
Gefahr durch Geräuschentwicklung.

EN

Schutzleiteranschluss. Das Symbol ist innerhalb des Gerätes angebracht. Dies ist ein Hinweis fürServicepersonal.

DE

Sicherung.
Elektro- und Elektronik-Altgeräte.

ES

An (Stromversorgung).
Aus (Stromversorgung)FR

Vor der Installation und Inbetriebnahme des Gerätes ist das Benutzerhandbuch zu lesen.

IT

Europäisches Konformitätskennzeichen
Britische Produktkennzeichnung
NRTL-Prüfzeichen: Das Produkt entspricht den im Konsensverfahren erarbeiteten Sicherheitsanforderungen,welche von der Occupational Safety/Health Administration (OSHA) gefordert und von der staatlich anerkannten
Prüfstelle (Nationally Recognized Testing Laboratories (NRTL)) TÜV SÜD geprüft wurden

Sicherheitskennzeichnungen
Der nachfolgende Sicherheitsaufkleber ist auf der Rückseite des Geräts befestigt. Seine Beachtung spielt für die
Aufrechterhaltung der Sicherheit eine wichtige Rolle.
WARNUNG! Vermeiden Sie schwere Verletzungen. Vor der Installation oder
Inbetriebnahme ist die Bedienungsanleitung zu lesen. Wenn die Anleitung
fehlt, fordern Sie Ersatz bei Miltenyi Biotec an.

14

EN

WICHTIGE SICHERHEITSINFORMATIONEN

Der Einsatz trägt folgendes Sicherheitssymbol.

DE

Figure 1: Sicherheitssymbol auf dem Einsatz des gentleMACS Octo Dissociator with Heaters. Die Sleeves tragen eine farblich

IT

FR

ES

abgesetzte Sicherheitsmarkierung.

Figure 2: Sicherheitssymbol auf den gentleMACS Heaters.

Die Aufsteckhülsen tragen eine farbliche abgesetzte Sicherheitsmarkierungen. Diese Sicherheitsmarkierung
ermöglicht die Rotation der Aufsteckhülsen optisch wahrzunehmen.
Alle Sicherheitsaufkleber und Markierungen müssen sauber und lesbar bleiben. Überprüfen Sie die Aufkleber
regelmäßig und ersetzen Sie diese, sollten sie aus sicherem Abstand nicht mehr lesbar sein. Kontaktieren Sie
Miltenyi Biotec um Ersatzaufkleber zu erhalten.

Allgemeine Sicherheitshinweise
Falls Ihr Gerät nicht einwandfrei funktioniert und/oder Anzeigen auf dem Display Sie dazu auffordern den
technischen Kundendienst zu kontaktieren, ist die Betriebssicherheit des Gerätes nicht länger gewährleistet.
Schalten Sie das Gerät sofort aus, ziehen Sie den Netzstecker und kontaktieren Sie einen autorisierten Miltenyi
Biotec Servicedienstleister oder den Miltenyi Biotec Technical Support.

Elektrische Gefährdung und Brandgefahr
Elektrische Geräte bergen das Risiko eines Stromschlags, Kurzschlusses und der Überhitzung. Ein
Stromschlag kann zu schweren Verletzungen und bis zum Tod führen. Ein elektrischer Kurzschluss
sowie Überhitzung kann eine Brandursache darstellen. Elektrische Geräte können Funken schlagen,

15

WICHTIGE SICHERHEITSINFORMATIONEN

welche leichtentflammbare Dämpfe oder Materialien entzünden und somit zu Feuer und Explosionen führen
können. Benutzen Sie das Gerät nicht in ausgewiesenen Gefahrenzonen, z. B. in sauerstoffangereicherten
Umgebungen.
Das Gehäuse des Gerätes dient dazu das Risiko eines elektrischen Stromschlags, Kurzschlusses und Feuers zu
reduzieren. Entfernen oder durchdringen Sie keine Abdeckungen, mit Ausnahme des Troughs und des Safety
Shields. Jegliche andere Abdeckung darf nur von autorisiertem Personal entfernt werden. Führen Sie nie einenDEEN

Fremdkörper durch eine Öffnung in das Gerät ein. Benutzen Sie das Gerät nicht, wenn:les offen oder zerlegt istles fallengelassen wurde oder beschädigt istlTeile beschädigt oder defekt sindldie Netzanschlussleitung beschädigt istlein Gegenstand in die Belüftungsschlitze eingedrungen istlein Fremdkörper in des Gerät gefallen ist.

Wenn Flammen oder Rauch auftreten, schalten Sie das Gerät unverzüglich ab, ziehen Sie den Netzstecker undESkontaktieren Sie einen autorisierten Miltenyi Biotec Servicedienstleister oder den Miltenyi Biotec TechnicalSupport.

FR

Das Gerät ist ausschließlich für den Betrieb in Innenräumen ausgelegt.
Sollten Flüssigkeiten in das Gerät gelangen, kann es zu Kurzschluss, Stromschlag oder Feuer kommen.

IT

Seien Sie vorsichtig im Umgang mit Flüssigkeiten. Verhindern Sie, dass Flüssigkeit in das Innere des Gerätes
gelangt. Schützen Sie das Gerät vor verschütteten oder verspritzten Flüssigkeiten.
Beseitigen Sie ausgetretene oder verschüttete Flüssigkeit sofort. Benutzen Sie das Gerät nicht, sollte Flüssigkeit
in das Innere des Gerätes gelangt sein. Setzen Sie das Gerät nicht in einer nassen oder feuchten Umgebung ein.
Vermeiden Sie Arbeitsbereiche mit hoher Luftfeuchtigkeit oder Kondensation. Das Überführen des Gerätes voneiner kalten Umgebung, z. B. aus einem Kühlraum bei 5°C, in einen Raum mit Raumtemperatur, kann zu
Kondensation im Inneren des Gerätes führen. Warten Sie in solchen Fällen mit der Benutzung des Gerätes, bis
dieses getrocknet ist. Ziehen Sie den Netzstecker vor der Reinigung des Gerätes. Verwenden Sie keine Flüssigoder Sprühreinigungsmittel, sondern nur ein feuchtes Tuch.
Ermöglichen Sie im Betrieb eine ausreichende Luftzirkulation. Die das Gerät umgebende Luft reicht nicht aus,das Gerät bei unzureichender Belüftung auf einer sicheren Betriebstemperatur zu halten. Ermöglichen Sie im
Betrieb eine ausreichende Luftzirkulation – einen Abstand von mindestens 15 cm in allen Richtungen um das
Gerät – um eine ausreichende Kühlung zu gewährleisten.
Das Gerät darf nicht in der Nähe von Radiatoren, Heißlüftern, Öfen, oder anderen Wärme erzeugenden Geräten
aufgestellt werden (Verstärker eingeschlossen). Schützen Sie das Gerät vor direkter Sonneneinstrahlung.
Öffnungen und Schlitze am Gerät sind zur Belüftung gedacht und sollten niemals bedeckt oder blockiert
werden, da sie das Gerät vor Überhitzung schützen und einen sicheren Betrieb ermöglichen. Versuchen Sie
nicht, das Gerät in eine Einbaukonstruktion zu integrieren oder auf begrenztem Raum unterzubringen, es seidenn, es wurde eine spezielle Vorrichtung entwickelt und für ausreichend Belüftung gesorgt. Befolgen Sie die
Montageanleitung für den Aufbau des Gerätes.
Stellen Sie sicher, dass der Hauptstromschalter ebenso wie der Netzstecker für das Stromkabel leicht
zugänglich sind und sich in möglichst unmittelbarer Nähe zum Bediener des Gerätes befinden. Sollte es
erforderlich sein, die Stromzufuhr zu unterbrechen, ziehen Sie den Netzstecker aus der Steckdose.

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WICHTIGE SICHERHEITSINFORMATIONEN

Das Gerät ist mit einem Schutzkontaktstecker und einer dreiadrigen Anschlussleitung ausgestattet. Dieser
Stecker passt nur in eine Steckdose mit Schutzkontakt. Das ist eine Sicherheitsvorrichtung. Versuchen Sie nicht,
den Stecker in eine Steckdose ohne Schutzkontakt einzuführen. Wenn Sie den Stecker nicht in die Steckdose
einführen können, bitten Sie einen ortsansässigen Elektriker, diese zu ersetzen.
Das Gerät sollte nur von einer Stromquelle aus betrieben werden, die den elektrischen Angaben auf dem
Typschild entspricht. Sollten Sie Fragen zur Art der Stromversorgung haben, wenden Sie sich an einen
autorisierten Miltenyi Biotec Servicedienstleister oder Ihren lokalen Stromversorger. Benutzen Sie keine
Verlängerungskabel oder Steckdosenleiste. Überlasten Sie eine Steckdose nicht.

Mechanische Gefahr

EN

Bewegliche und rotierende Teile sind potentielle mechanische Gefahrenquellen. Benutzen Sie das Gerät nicht
ohne befestigtes Trough, Sleeves und Safety Shield. Wenn sich die Rotoren des Gerätes bewegen, berühren Sie

DEdiese nicht und laden oder entfernen Sie keine gentleMACS Tubes vom Gerät. Benutzen Sie nur korrekt
installierte gentleMACS Tubes mit fest verschlossenem Deckel. Benutzen Sie das Gerät nicht mit nur
aufgesteckten Deckeln der gentleMACS Tubes. Die freiliegenden scharfen Kanten der Deckel der gentleMACS

ES

Tubes können Schnittwunden verursachen, wenn diese im Betrieb berührt werden. Umgehen Sie keine
Schutzmaßnahmen oder Sicherheitsvorrichtungen.

FR

Chemische und biologische Gefahren
Abhängig von dem benutzten biologischen Material kann eine Kontamination oder Infektion zu schweren

IT

Verletzungen oder Tod führen. Klinische Proben müssen als potentiell infektiös angesehen werden. Wird oderwurde mit biologischen Gefahrstoffen gearbeitet, muss der Bediener des Gerätes entsprechend den
für die verwendeten Substanzen geltenden Warnhinweisen und Schutzbestimmungen eine persönliche
Schutzausrüstung tragen. Der oben genannte Sicherheitshinweis gilt auch für etwaige gefährliche
Chemikalien, einschließlich toxischer oder korrosiver Chemikalien, ätzender oder radioaktiver Substanzen, diein der Probe anwesend sein können. Tragen Sie Schutzhandschuhe, Schutzkleidung und Schutzbrille, um
Berührung der Gefahrstoffe mit Haut und Augen zu vermeiden. Mangelhafte oder unzureichende
Schutzausrüstung ist gefährlich. Werden Gefahrstoffe oder unbekannte Substanzen eingesetzt, muss das Gerät
unter einem Abzug oder einer Sicherheitswerkbank benutzt werden. Falls Gefahrstoffe verwendet wurden oderausgetreten sind, muss auf eine sorgfältige Dekontamination des Gerätes geachtet werden. Es ist strengstensverboten, kontaminierte Geräteteile oder Zubehörteile weiter zu verwenden.
Röhrchen und alle weiteren Verbrauchsmaterialien, die in Kontakt mit biologischen Gefahrstoffen gelangt sind,müssen vor dem Entsorgen dekontaminiert werden. Alle flüssigen und festen Abfälle müssen als Gefahrstoffe
angesehen werden. Die Handhabung der Abfälle muss daher unter Beachtung der allgemein geltenden
Laborsicherheitsbestimmungen erfolgen. Die Entsorgung der Verbrauchsmaterialien muss gemäß denörtlichen Bestimmungen erfolgen.

Gefahr durch optische Strahlung
Das Gerät ist mit leistungsstarken lichtemittierenden Dioden (LEDs) ausgestattet, welche das Safety Shield unddie gentleMACS Tubes beleuchten. Gemäß der internationalen Norm IEC 62471 hat dieses Lampensystemeinen Gefahrenwert der Exposition (EHV) von 0,91 und fällt in die Freie Gruppe. Der Gefährdungsabstand (HD)für die Freie Gruppe beträgt 61 cm. Der Gefährdungsabstand für die Risikogruppe 1 beträgt 20 cm. Das Risikofür den Betrachter ist abhängig von der Installation und Benutzung des Gerätes. Die von den LEDs emittierte
optische Strahlung kann gefährlich für die Augen sein, wenn man aus sehr geringer Entfernung in das Gerät

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WICHTIGE SICHERHEITSINFORMATIONEN

mit entferntem Trough schaut. Ziehen Sie den Netzstecker des Gerätes bevor Sie den Trough entfernen. Wenn
der Trough während des Betriebs vom Gerät entfernt wird, blicken Sie aus einer Entfernung von weniger als 20
cm nicht direkt in das Licht der LEDs.
Ferner werden LEDs für die Beleuchtung des Lesebereiches eines integrierten 2D Code Lesegeräts benutzt.
Blicken Sie im Betrieb nicht aus kurzer Entfernung direkt auf das 2D Code Lesegerät.
Nehmen Sie optische Strahlungsquellen nicht auseinander. Verändern oder entfernen Sie diese nicht. Die
optische Strahlung aus geöffneten Geräten kann für die Augen schädlich sein.

Gefahr durch heiße Oberfläche

EN

Die Heizfolie kann ausreichend heiß werden, um bei Berührung Verbrennungen zu verursachen. Die Heizfolie
darf nicht berührt werden, während das Gerät in Betrieb ist.

DE

Gefahr durch Geräuschentwicklung
Wird das Gerät bei maximaler Beladung benutzt, kann es zu einer Geräuschentwicklung kommen, dessen PegelESeine Gesundheitsgefährdung darstellt. Gefährliche Schalldruckpegel können zu Hörschäden führen. Es wird
empfohlen, dass der Betreiber den Schalldruckpegel am Benutzerstandort und am Ort des höchsten
Schalldruckpegels im Umkreis von 1 m des Gerätes misst und berechnet. Es sollten schützenden Maßnahmen,FRwie z. B. schalldämpfende Lärmschutzvorrichtung, Lärmschutzhauben, oder Gehörschutz benutzt werden,
wenn während der Installation des Gerätes nicht sichergestellt werden kann, dass der Schalldruckpegel desGerätes am Standort der Benutzung einen den die Gesundheit gefährdenden Wert nicht übersteigt. BeachtenIT

Sie lokale Vorgaben zum Gehörschutz.

Wartung, Transport und Geräteentsorgung
Versuchen Sie nicht, das Gerät selbst zu warten oder zu reparieren – es sei denn, es ist in diesem
Benutzerhandbuch oder anderen technischen Unterlagen von Miltenyi Biotec ausdrücklich vermerkt. Wartung
und Reparaturen müssen durch geschulte Fachkräfte ausgeführt werden. Falsche oder unsachgemäße
Wartung oder Reparatur an Ihrem Gerät kann zur Gefährdung des Anwenders, unvorhersehbaren Resultaten,
Fehlfunktionen, Geräteschäden, vorzeitigem Verschleiß und verringerter Lebensdauer führen. Es kann auchden Verlust Ihrer Garantieansprüche zur Folge haben.
Wenn ein Austausch oder Ersatzteile benötigt werden, stellen Sie sicher, dass Ihr Servicedienstleister nur
Originalteile von Miltenyi Biotec oder Teile von Drittanbietern verwendet, die von Miltenyi Biotec spezifiziert
und empfohlen werden. Die Verwendung unautorisierter Ersatzteile kann Fehlfunktionen des Gerätes
verursachen und die Ergebnisse beeinträchtigen. Miltenyi Biotec akzeptiert keinerlei Garantieansprüche undhaftet nicht für Fehlfunktionen oder Schäden am Gerät, die auf Verwendung ungeeigneter Verschleiß- oder
Ersatzteile zurückzuführen sind.
Stellen Sie sicher, dass nach jeder erfolgten Wartungs- oder Reparaturleistung ein autorisierter Miltenyi Biotec
Servicedienstleister alle notwendigen Sicherheitsprüfungen, welche die durchgeführten Reparaturmaßnahmen
verlangen, durchführt, um sicherzustellen, dass das Gerät sich in vorschriftsmäßigem Zustand befindet.
Nutzen Sie nur von Miltenyi Biotec empfohlenes Zusatzgerät und Upgrades zu Ihrem Gerät. Fragen Sie
Ihren örtlichen Miltenyi Biotec Vertriebsmitarbeiter nach Miltenyi Biotecs weit reichenden Vereinbarungen zum
Geräteservice und Technical Support oder besuchen unsere Website: www.miltenyibiotec.com/support.

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WICHTIGE SICHERHEITSINFORMATIONEN

Das Gerät sollte vorsichtig gehandhabt in der von Miltenyi Biotec bereit gestellten Verpackung transportiert
werden. Im Gerät können innere Schäden auftreten, falls es großer Erschütterung ausgesetzt oderfallengelassen wird. Sollte wegen Reparatur- oder Wartungsleistungen ein Rücktransport zum Hersteller
notwendig sein, dekontaminieren Sie das Gerät vor dem Versand, um jegliche Gefahrenstoffe zu entfernen.
Wenn Sie Fragen zur vorschriftsmäßigen Dekontaminierung oder zum Versand des Gerätes haben, wenden Sie
sich zur Unterstützung bitte direkt an unseren Miltenyi Biotec Technical Support.

Kundeninformation zur Entsorgung von Elektro- und Elektronik-Altgeräten (Waste of Electricaland Electronic Equipment, WEEE)
Bitte entsorgen Sie Ihre Altgeräte von Miltenyi Biotec unter Einhaltung der jeweils geltenden Vorschriften fürdie Erfassung und Behandlung von Elektro- und Elektronik-Altgeräten und die Entsorgung von Gefahrstoffen.

EN

Diese können von Land zu Land sowie regional variieren.
Elektrische und elektronische Geräte können Gefahrstoffe enthalten, welche die Umwelt erheblich belastenDE

und/oder die Gesundheit gefährden. Deshalb müssen Altgeräte speziell gesammelt und durch ausgewiesene
Entsorgungsbetriebe im Rahmen der hierfür vorgesehenen Entsorgungssysteme fachgerecht behandeltESwerden. Indem Sie sicherstellen, dass Ihr Altgerät gemäß den geltenden Vorschriften zur Behandlung vonElektro- und Elektronik-Altgeräten sowie von Gefahrstoffen entsorgt wird, tragen Sie dazu bei, unsereFR

natürlichen Ressourcen zu schonen und die menschliche Gesundheit zu schützen.
Miltenyi Biotec setzt sich für den Schutz der Umwelt ein. Miltenyi Biotec bietet in zahlreichen Ländern eigene
Rücknahmeprogramme für Altgeräte an und arbeitet weltweit mit lizensierten Partnern zusammen, die anITbestehende Recycling- und Entsorgungssysteme angeschlossen sind. Miltenyi Biotec ermöglicht Ihnen ein
kostenloses Recycling Ihres Altgerätes. Die Bedingungen und die Verfügbarkeit dieses Angebots unterscheiden
sich geographisch aufgrund unterschiedlicher regulatorischer Anforderungen. Bitte beachten Sie, dass je nach
Art und Nutzung Ihres Gerätes zusätzliche Anforderungen gelten können.
Für weitere Informationen oder wenn Sie Ihr Miltenyi Biotec-Altgerät entsorgen möchten, wenden Sie sichbitte an Ihren lokalen Miltenyi Biotec-Vertreter oder den Miltenyi Biotec Technical Support.

19

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FR

ES

DE
EN

WICHTIGE SICHERHEITSINFORMATIONENINFORMACIÓN IMPORTANTE DE SEGURIDAD

ESInformación importante de seguridad

EN

El gentleMACS Octo Dissociator with Heaters es un aparato de laboratorio. El instrumento se puede utilizar deforma segura si se garantiza lo siguiente: el instrumento está instalado correctamente. El instrumento solo lomanejan personal de laboratorio profesional. Se siguen las prácticas generales de seguridad. Se siguen todasDE

las instrucciones en este manual de uso.

ES

Advertencias y precauciones
Las directrices en esta sección explican los riesgos potenciales asociados con el manejo de este instrumento yproporcionan una importante información complementaria para minimizar los riesgos. Siga las instrucciones

FR

atentamente para protegerse a sí mismo, a otros y al equipamiento, de riesgos potenciales y crear un ambientede trabajo seguro. Use este instrumento solo como especificado por el fabricante para evitar daños en elITequipo y lesiones en el personal.
Siga siempre las instrucciones de seguridad y política de laboratorio del área de trabajo local, así como losestándares de salud, seguridad y prevención de accidentes. Contacte la autoridad local a cargo de la fuente deenergía eléctrica, construcciones, mantenimiento o seguridad para más información sobre la instalación seguray manejo del instrumento.

Niveles de riesgo
Se usan señales para identificar mensajes de seguridad y de daño de la propiedad. Las siguientes señales seusan a través de este manual de usuario.
o WARNING! indica una situación potencialmente peligrosa, la cual, si no se evita, puede resultar en muerte odaños graves.
o CAUTION! indica una una situación potencialmente peligrosa, la cual, si no se evita, puede resultar en un
daño menor o moderato. También puede ser usado para alertar contra prácticas peligrosas.

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INFORMACIÓN IMPORTANTE DE SEGURIDAD

SímbolosLos siguientes símbolos se usan para destacar condiciones que podrían causar daños al personal o daños alequipo:Alerta de seguridad. Riesgo de peligro. Es necesario consultar la documentación del equipo siempre que este
símbolo de alerta de seguridad aparezca, para de esta manera poder averiguar la naturaleza del peligro
potencial existente y las acciones necesarias a realizar.
Riesgo biológico. Riesgo de contaminación si se usa material biológico potencialmente peligroso.

EN

Riesgo de shock eléctrico.
Radiación óptica peligrosa.

DE

Riesgo involuntario de captura de dedo por parte rotatoria peligrosa.
Peligro superficie caliente.

ES

Riesgos del nivel de ruido.

FR

Bloques de conexión para conductores de protección. El símbolo está pegado en el interior del instrumento.
Esta información es para el personal de servicio.
Fusible.

IT

RAEE (Residuos de Aparatos Eléctricos y Electrónicos).

ON (Fuente de energía).
OFF (Fuente de energía).
Lea todas las instrucciones de uso antes de instalar y utilizar el instrumento.
Aprobación de Conformidad Europea
Conformidad evaluada del Reino Unido.
Marca de certificación NRTL: El producto cumple los estándares de seguridad consensuados, requeridos por
la Administración de Seguridad y Salud Ocupacional (OSHA), determinados por el laboratorio de ensayo
reconocido nacionalmente (Nationally Recognized Testing Laboratories (NRTL)) TÜV Süd.

Etiqueta de seguridad
La siguiente etiqueta de seguridad se encuentra pegada a la parte posterior del instrumento y juega un papel
importante en el mantenimiento de la seguridad.
ADVERTENCIA! Evita las lesiones graves. Lea y comprenda las instrucciones
de funcionamiento antes de la instalación o el uso. Si falta el manual,
solicite su sustitución a Miltenyi Biotec.

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INFORMACIÓN IMPORTANTE DE SEGURIDADEN

La base presenta los siguientes símbolos de seguridad.

DE

Figure 1: Símbolo de seguridad en la base del gentleMACS Octo Dissociator with Heaters. Los manguitos están marcados con una

IT

FR

ES

impresión de contraste para seguridad.

Figure 2: Símbolo de seguridad en los calefactores gentleMACS.

Los manguitos están marcados con una impresión de contraste para seguridad. Esta impresión de contrastepara seguridad permite la percepción visual de los manguitos rotatorios.
Las etiquetas de seguridad arriba mencionadas y las marcas de seguridad deberán mantenerse límpias ylegibles. Inspecionar periódicamente las etiquetas y marcas de seguridad y reemplazarlas en caso de que estas
no sean legibles o perceptibles a una distancia visual segura. Contacte con Miltenyi Biotec para reemplazo deetiquetas.

Instrucciones generales de seguridad
Si el instrumento no funciona correctamente y/o las instrucciones o mensajes mostrados le aconsejan
contactar con el servicio técnico, no es posible utilizar el instrumento de una manera segura. Apague ydesenchufe inmediatamente el instrumento de la toma de corriente, y contacte un proveedor de servicios
autorizado de Miltenyi Biotec o un servicio técnico autorizado.

Incendios y riesgos eléctricosLos riesgos potenciales causados por aparatos eléctricos incluyen shock eléctrico, cortocircuito ysobrecalentamiento. Un shock eléctrico puede llevar a graves daños corporales o incluso la muerte. Un

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INFORMACIÓN IMPORTANTE DE SEGURIDAD

cortocircuito eléctrico o sobrecalentamiento puede ser causa de incendio. El equipo electrónico puede
emitir chispas, las cuales podrían encender vapores o material combustible resultando en explosión o incendio.
No use el instrumento en áreas designadas como peligrosas; por ejemplo, en medios cargados de oxígeno.
La caja protectora del instrumento está diseñada para reducir el riesgo de shock eléctrico, cortocircuito
eléctrico y propagación de fuego. Excepto la de la Safety Shield y la Trough, no quite o penetre ninguna tapa ocubierta. Solo el personal autorizado debe quitar las otras tapas del instrumento. Nunca introduzca un objeto

DEEN

extraño a través de una apertura en el instrumento. No use el instrumento silestá abierto o desensamblado,lse ha caído o ha sido dañado,ltiene partes dañadas o rotas,ltiene un cable de energía dañado,l

un objeto ha entrado a través de las ranuras de ventilación,l

un objeto extraño ha caído en el instrumento.

Si aparecen llamas o humo, inmediatamente apague la fuente de energía, desenchufe el instrumento de laEStoma de corriente, y contacte un proveedor de servicios autorizado de Miltenyi Biotec o un servicio técnico deMiltenyi.

FR

El instrumento está destinado solo a uso en un lugar cubierto. Si algún fluido entra en el instrumento,
puede ocasionar cortocircuitos eléctricos, shock eléctrico o incendio. No permita que fluidos penetren elITinterior del instrumento. Proteja el instrumento contra vertidos y salpicaduras accidentales. Limpie
inmediatamente los vertidos. No utilice el instrumento si se han derramado líquidos en el instrumento. No
utilice el instrumento en un lugar mojado o húmedo. Evite áreas con alta humedad o vapor. Transportar el
instrumento de un medio frío – como una habitación fría a 5 °C – a temperatura ambiente, puede causar una
condensación dentro del instrumento. En esos casos, espere a que el instrumento se deshumidifique antes deutilizarlo. Desenchufe el instrumento de la toma de corriente antes de limpiarlo. No utilice agentes limpiadoreslíquidos o aerosoles; use siempre un paño húmedo.
Asegúrese de que hay suficiente ventilación en el lugar donde se utilice el instrumento. En la ausencia de una
circulación adecuada, el aire del ambiente puede no enfriar el instrumento a temperaturas de funcionamiento
adecuadas. Permita una suficiente circulación del aire alrededor del instrumento – al menos 15 cm en todos loslados – durante el manejo para asegurar un enfriamiento adecuado. No coloque el instrumento cerca deradiadores, registros de calor, estufas, u otro equipamiento (incluyendo amplificadores) que genere calor.
Proteja el instrumento de la luz directa del sol. No cubra las ranuras y aperturas del instrumento ya que están
destinadas a la ventilación y protección contra el sobrecalentamiento del instrumento. No coloque elinstrumento en un estante empotrado o espacios similarmente confinados a menos que el espacio haya sido
específicamente designado para proveer una ventilación adecuada. Siga las instrucciones de montaje delinstrumento.
Asegúrese de que el enchufe principal así como el conector para el cable de energía están fácilmente
accesibles y colocados tan cerca del operador del instrumento como sea posible. Si es necesario desconectar lafuente de energía, desconecte el cable de la toma de corriente.
El instrumento está equipado con un enchufe eléctrico con tres cables para la toma de tierra, que tiene una
tercera clavija para la toma. Este enchufe solo se adapta a una toma de corriente de tierra. Este es unprocedimiento de seguridad. No intente insertar el enchufe en una toma de corriente que no sea de tierra. Si
no puede insertar el enchufe en la toma, contacte a su electricista local para reemplazar la toma.

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INFORMACIÓN IMPORTANTE DE SEGURIDAD

El instrumento debería ser utilizado desde una fuente de energía que cumple las especificaciones mencionadas
en la etiqueta de potencia eléctrica del producto.
Si tiene preguntas sobre el tipo de fuente de energía que usar, contacte a su proveedor de servicios autorizado
de Miltenyi Biotec o una compañía de energía local. No use alargadores o enchufes múltiples. No sobrecargue
una toma de corriente.

Riesgos mecánicosMover y quitar piezas son potenciales riesgos mecánicos. Cuando utilice el instrumento, mantenga la Trough,Sleeves, y Safety Shield junto a él. No toque o quite los tubos del instrumento cuando los rotores delinstrumento estén rotando. Utilice solo gentleMACS Tubes instalados adecuadamente con tapas fuertementecerradas. No utilice el instrumento con solo las tapas de los gentleMACS Tubes en su lugar. Los bordesEN

cortantes de las tapas de los gentleMACS Tubes pueden llevar a heridas por corte si se tocan mientras estánDE

rotando. No ignore ninguna medida o aparato de seguridad.

Riesgos químicos y biológicos

ES

Dependiendo del material biológico utilizado, una contaminación o infección puede llevar a graves daños
personales o muerte. Todas las muestras clínicas deben ser consideradas potencialmente infecciosas. Si se ha
usado material de riesgo biológico, el operador debe elegir y llevar puesto equipamiento personal deFRseguridad como indicado en las advertencias y precauciones para la substancia particular. La precaución deseguridad anterior también es válida para cualquier químico de riesgo, incluyendo químicos tóxicos ocorrosivos, ácidos, o substancias radioactivas que pueden estar presentes en la muestra. Lleve guantes, ropa yITgafas de seguridad para prevenir el contacto de la substancia con la piel y ojos. Un equipamiento de seguridad
defectuoso o inadecuado es peligroso. El instrumento debe ser utilizado con una cubierta de seguridad si están
siendo procesados materiales peligrosos o desconocidos. Si se ha utilizado o derramado material peligroso,debe tenerse cuidado en descontaminar a fondo el instrumento. Está estrictamente prohibido continuar
utilizando accesorios o piezas del instrumento contaminadas.
Los tubos y otros consumibles que han estado en contacto con material de riesgo biológico deben serdescontaminados antes de su eliminación. Todos los residuos líquidos o sólidos deben ser considerados
peligrosos de acuerdo con las precauciones universales de laboratorio. El desecho de residuos debe hacerse deacuerdo con las regulaciones locales.

Riesgos de radiación óptica
El instrumento está equipado con poderosos diodos de emisión de luz (LEDs) para iluminar la Safety Shield ylos gentleMACS Tubes. De acuerdo con el estándar internacional IEC 62471, este sistema de luces tiene unvalor de riesgo por exposición (EHV) de 0.91 y es superior al Grupo Exento de Riesgo. La distancia de riesgo(HD) para el Grupo Exento de Riesgo es 61 cm. La distancia de riesgo para el Grupo de Riesgo 1 es 20 cm. El
riesgo del visor depende de cómo se instala y utiliza el instrumento. La radiación óptica emitida por estos LEDs
puede ser dañina para los ojos a distancias cortas si se quita la Trough. Desenchufe el instrumento antes dequitar la Trough. Si se quita la Trough, no mire fijamente la iluminación de los diodos LEDs en funcionamiento
desde una distancia menor a 20 cm.
Además, el lector de códigos 2D integrado utiliza LEDs para iluminar el área de lectura. No mire directamente al
lector de códigos 2D desde una distancia corta mientras está en funcionamiento.
No desensamble, modifique, o elimine ninguna de las fuentes de radiación óptica. La radiación óptica deinstrumentos desensamblados puede ser perjudicial para los ojos.

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INFORMACIÓN IMPORTANTE DE SEGURIDAD

Riesgos superficie caliente
La lámina calefactada de los calenfactores gentleMACS puede llegar a causar quemaduras si se toca. No tocar lalámina calefactada mientras el equipo está en funcionamiento.

Riesgos del nivel de ruido
Si el instrumento se usa en su carga máxima, puede producir problemas de oído. Se recomienda que elorganismo responsable mida o calcule el nivel de presión del sonido en la posición del operador, y en cualquierpunto alejado 1 metro del equipo, donde tiene su nivel de presión más alto. Si no se asegura durante lainstalación que el nivel de presión del sonido del aparato no alcanzará en este punto de uso un valor que
podría suponer un riesgo, entonces debe usarse equipamiento de protección como cubiertas reductoras delEN

ruido. Observe las normas locales sobre protección auditiva.

DE

Servicio, transporte y eliminación seguros
A menos que de otro modo señalado en este manual de usuario o en otra documentación de Miltenyi Biotec,
no repare o revise el instrumento Usted mismo. Las revisiones y reparaciones deben ser llevadas a cabo por

ESpersonal de servicio cualificado. Una reparación o revisión inadecuada o incorrecta del instrumento puedecausar riesgos a los usuarios, llevar a resultados impredecibles, causar el mal funcionamiento o daño delinstrumento, así como un prematuro desgaste y vida reducida del instrumento. También puede invalidar su

FRgarantía.
Cuando sean requeridas piezas de repuesto, asegúrese de que el proveedor de servicios usa solo piezas

IT

originales de Miltenyi Biotec, o piezas de terceros especificadas y recomendadas por Miltenyi Biotec. El uso depiezas no autorizadas puede causar un mal funcionamiento del instrumento y resultados dañados. Miltenyi
Biotec no se hace responsable de ninguna garantía o de un error o daño del instrumento resultante del uso depiezas inadecuadas. Al finalizar cualquier servicio o reparación, asegúrese de que su proveedor de servicios
autorizado de Miltenyi Biotec lleva a cabo todas las comprobaciones de seguridad como requerido por elprocedimiento de reparación, para asegurarse de que el instrumento está funcionando correctamente.
Utilice solo opciones y actualizaciones recomendadas por Miltenyi Biotec. Consulte con su representante local
de Miltenyi Biotec sobre el amplio servicio del instrumento, o diríjase a www.miltenyibiotec.com/support.
El instrumento debe ser transportado cuidadosamente en embalaje especificado por Miltenyi Biotec. Si el
instrumento está sujeto a una vibración excesiva o caída, pueden ocurrir daños internos. Si el instrumento
necesita ser enviado de vuelta al fabricante para su servicio, descontamine el instrumento para eliminar
cualquier material peligroso antes del transporte. Si tiene preguntas respecto a una correcto
descontaminización o transporte, contacte al Servicio Técnico Miltenyi Biotec.

Información sobre la Directiva de Residuos de Aparatos Eléctricos y Electrónicos (Waste ofElectrical and Electronic Equipment, WEEE)
Por favor, al final de la vida útil de su equipo de Miltenyi Biotec disponga del mismo de acuerdo a la ley WEEE
aplicable, la cual puede ser diferente según el país o región.
Los equipos eléctricos y electrónicos pueden contener sustancias peligrosas que pueden tener graves efectos
perjudiciales sobre el medio ambiente y/o la salud humana. Por este motivo, todos los equipos deben ser
específicamente recogidos y tratados por los centros de residuos designados y según planes de cumplimientode la WEEE cualificada. Al asegurarse de que se está deshaciendo de su equipo eléctrico y electrónico no
deseado de acuerdo con la legislación y la WEEE aplicable de disposición de residuos peligrosos, estará
ayudando a preservar nuestros recursos naturales y a proteger la salud humana.

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INFORMACIÓN IMPORTANTE DE SEGURIDAD

Miltenyi Biotec está comprometido con la protección del medio ambiente. Miltenyi Biotec ofrece productosque se encuentran en el final de su vida a programas de retorno de muchos países, y a socios con esquemas
de cumplimiento de licencias WEEE en todo el mundo. Miltenyi Biotec le ofrece reciclar su equipo de Miltenyi
Biotec en el final de su vida útil forma gratuita. Los términos y la disponibilidad de esta oferta pueden variar
según la región geográfica, debido a las diferencias en los requisitos reglamentarios. Tenga en cuenta que,dependiendo del tipo y uso del equipo, pueden aplicarse requisitos adicionales.
Para más información, o si desea eliminar su equipo de Miltenyi Biotec en el final de su vida útil, por favor

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póngase en contacto con su representante local de Miltenyi Biotec o con el Servicio Técnico de Miltnyi Biotec.

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INFORMACIÓN IMPORTANTE DE SEGURIDAD

INFORMATIONS DE SÉCURITÉ IMPORTANTESFRInformations de sécurité importantes

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Le gentleMACS Octo Dissociator with Heaters est un appareil de laboratoire. L’instrument peut être utilisé entoute sécurité si les conditions suivantes sont réunies : L’instrument est correctement installé. L’instrument est
utilisé uniquement par des personnel de laboratoire professionnel. Les pratiques de sécurité générales sontDEobservées. Toutes les instructions fournies dans ce Guide de l’Utilisateur sont observées.

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Avertissements et précautions
Les directives dans ce chapitre décrivent les risques potentiels associés au maniement de cet appareil etfournissent d’importantes informations de sécurité supplémentaires pour réduire les risques. Suivez

FRsoigneusement les instructions, afin de vous protéger vous-même, des tiers et le dispositif d’éventuels dangers
et de créer un environnement de travail sûr. Utilisez cet appareil uniquement en respectant les instructions duITfabricant afin d’éviter une détérioration du dispositif et des blessures du personnel.
Suivez toujours les consignes de sécurité du champ d’opération et les bonnes pratiques de laboratoire ainsi
que les normes pour la santé, la sécurité et la prévention d’accidents. Contactez les autorités locales gérant
l’alimentation en courant électrique, la construction de bâtiments, la maintenance ou la sécurité pour plusd’informations sur l’installation et l’utilisation sûre de l’appareil.

Niveaux de danger
Des mots clé sont utilisés pour identifier des messages sur des détériorations matériels et de sécurité. Les mots
clé suivants sont utilisés dans ce manuel.
ou WARNING! indique une situation potentiellement dangereuse, pouvant entraîner la mort ou des blessures
graves, si elle n’est pas évitée.
ou CAUTION! indique une situation potentiellement dangereuse pouvant causer des blessures mineures ou
modérées, si elle n’est pas évitée. Il peut également servir d’alerte contre une utilisation risquée.

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SymbolesLes symboles suivants sont utilisés pour souligner des conditions qui pourraient entraîner des blessures dupersonnel ou une détérioration du dispositif :Alerte de sécurité. Risque de danger. La documentation doit être consulté dans les cas où un symbole d’alertede sécurité est mentionné sur l’instrument afin de connaitre la nature du danger potentiel et les mesures à
prendre.
Danger biologique. Risque de contamination, si des matières biologiques potentiellement dangereuses sontutilisés.
Risque d'un choc électrique.

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Radiation optique dangereuse.

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Risque de capture involontaire d’un doigt par rotation d’une partie dangereuse.
Surface dangereusement chaude.

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Niveaux de bruits dangereux.

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Borne pour conducteur de protection. Ce symbole est fixé à l’intérieure de l’appareil. Cette information est
destinée au personnel de service.
Fusible.

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DEEE (Déchets d'Équipements Électriques et Électroniques).
ON (Alimentation électrique).
OFF (Alimentation électrique).

Les instructions de service doivent être lues dans leur intégralité, avant l’installation et l’utilisation de l’appareil.

Approbation Européenne de Conformité
Marquage de produit du Royaume-Uni
Marque de certification NRTL : Le produit répond aux normes de sécurité consensuelles imposées parl’Occupational Safety/Health Administration (OSHA) et déterminées par le laboratoire d’essais reconnu au
niveau national (Nationally Recognized Testing Laboratories (NRTL)) TÜV Süd

Étiquette de sécurité
L’étiquette de sécurité est fixée à l’arrière de l’appareil et joue un rôle important pour la maintenance desécurité.
AVERTISSEMENT! Évitez les blessures graves. Lisez et comprenez les
instructions d'utilisation avant d'installer ou d'utiliser l'appareil. Si le manuelest manquant, demandez à Miltenyi Biotec de le remplacer.

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INFORMATIONS DE SÉCURITÉ IMPORTANTESDEEN

La cuve est marquée avec les symboles de sécurité suivants.

Figure 1: Symbole de sécurité sur la fosse du gentleMACS Octo Dissociator with Heaters. Les manchons bénéficient d’un marquage

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coloré.

Figure 2: Symboles de sécurité relatifs à la partie chauffante du gentleMACS.

Les manchons présentent un marquage coloré afin de signaler visuellement la partie rotative.
Les étiquettes et marquage de sécurité doivent être maintenus propres et lisibles. Il convient de les inspecter
périodiquement et de les remplacer si elles ne sont pas lisibles ou perceptibles à une distance d’observation
garantissant la sécurité du manipulateur. Contactez Miltenyi Biotec pour le remplacement des étiquettes desécurité.

Consignes de sécurité générales
Si l’appareil ne fonctionne pas correctement et/ou les instructions ou messages affichés vous avisent decontacter le support technique, l’utilisation sûre de l’appareil n’est plus longtemps garantie. Éteignez
immédiatement l’appareil et débranchez-le de la prise électrique, puis contactez un prestataire de services
Miltenyi Biotec agréé ou l’équipe de support technique de Miltenyi Biotec.

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INFORMATIONS DE SÉCURITÉ IMPORTANTESDangers électriques et incendie
Les risques potentiels résultant d’appareils électriques sont le choc électrique, le court circuit et lasurchauffe. Un choc électrique peut entraîner de graves blessures corporelles ou même la mort. Un
court circuit ou une surchauffe peuvent provoquer un incendie.Des équipements électriques peuventémettre des étincelles, pouvant enflammer des vapeurs ou matières combustibles, ce qui pourrait engendrer
une explosion ou un incendie. N’utilisez pas cet appareil dans des endroits jugés dangereux; par exemple, dansun environnement riche en oxygène.
Le boîtier de l’appareil est conçu de façon à réduire les risques de choc électrique, de court circuit et depropagation du feu. A l’exception du Safety Shield et de la Trough, n’enlevez aucun couvercle et n’y pénétrez
pas. Seul un personnel autorisé a le droit d’enlever les autres couvercles. N’introduisez jamais un corpslil est ouvert ou démonté,lil est tombé ou qu’il a été endommagé,lil a des pièces endommagées ou abîmées,lil a des câbles électriques endommagés,l

un objet y est entré par les fentes de ventilation,l

un corps étranger a pénétré dans l’appareil.

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étrangers à travers d’une fente à l’intérieure de l’appareil. N’utilisez jamais l’appareil lorsqu’Si des flammes ou la fumée apparaissent, coupez immédiatement l’alimentation électrique, débranchez
l’appareil de la prise électrique, puis contactez un prestataire de services Miltenyi Biotec agréé ou l’équipe deITsupport technique de Miltenyi Biotec.
L’appareil est uniquement destiné à une utilisation intérieure. Si des fluides pénètrent à l’intérieur del’appareil, ceci peut entraîner des courts circuits, des chocs électriques ou des incendies. Faitesextrêmement attention lorsque vous utilisez des liquides. Veillez à ce qu’aucun fluide ne pénètre à l’intérieur
de l’appareil. Protégez l’appareil contre des déversements et éclaboussements accidentels. Nettoyez
immédiatement tout déversement. Ne pas utiliser l’appareil, si des liquides ont coulés dans l’appareil. Ne pas
utiliser l’appareil dans des endroits humides et brumeux. Evitez des endroits ayant une forte humidité ou
condensation. Le déplacement de l’appareil d’un environnement froid – comme une chambre froide à 5 °C – à
un endroit à température ambiante peut causer une condensation à l’intérieur de l’appareil. Dans ces cas-là,
attendez que l’appareil soit sec, avant de l’utiliser. Débranchez l’appareil de la prise électrique avant de lenettoyer. Ne pas utiliser des détergents liquides ou aérosols; utilisez toujours un tissue humide.
Assurez une circulation adéquate de l’air dans la pièce où l’appareil est utilisé.
Si une telle circulation adéquate n’est pas assurée, l’air ambiant ne peut pas refroidir l’appareil à destempératures de fonctionnement acceptable. Veillez à ce que l’air puisse bien circulé au tour de l’appareil
pendant son fonctionnement – au moins 15 cm des deux côtés – pour garantir un refroidissement adéquat.
Évitez de placer l’appareil près de radiateurs, d’accumulateurs de chaleur, de poêles ou d’autres dispositifs (ycompris des amplificateurs) générant de la chaleur. Ne pas exposer l’appareil au soleil. Ne couvrez pas lesfentes et les lumières de l’appareil, car elles servent de ventilation et évite une surchauffe de l’appareil. Ne
placez pas l’appareil dans un rack encastré ou des espaces confinés similaires, à moins que cet espace ait
spécialement été conçu, pour fournir une ventilation convenable. Suivez les instructions de montage de cetappareil.
Assurez que l’interrupteur général ainsi que le connecteur pour le câble électrique soient facilement
accessibles et qu’ils se trouvent le plus proche possible de l’opérateur de l’appareil. S’il est nécessaire de couper
l’alimentation électrique, débranchez le câble de la prise électrique.

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L’appareil est équipé d’une prise électrique à trois fils et mise à la terre avec une troisième broche deconnecteur pour la mise à la terre. Cette prise ne s’emboîte que dans une prise de courant mise à la terre, ceci
pour des raisons de sécurité. N’essayez pas d’introduire la prise dans une prise femelle de courant non mise à laterre. Si vous ne parvenez pas à introduire la prise dans une prise femelle, contactez votre électricien local pourremplacer la prise femelle.
L’appareil doit uniquement être utilisé à partir d’une source d’énergie qui remplit les spécifications
mentionnées sur l’étiquette de puissance électrique du produit.
Si vous avez des questions sur le type de source de courant à utiliser, contactez votre prestataire de services
Miltenyi Biotec agréé ou votre entreprise locale de production et de distribution d’énergie. N’utilisez aucuneENrallonge électrique ou multiprise. Ne surchargez pas une prise électrique.

Dangers mécaniquesDE

Des pièces mobiles et tournantes sont de potentiels dangers mécaniques. Lorsque vous utilisez l’appareil,veillez à ce que la Trough, les Sleeves et le Safety Shield y soient bien fixés. Quand les rotors de l’appareil sonten rotation, ne les touchez pas. Ne chargez et n’enlevez pas les tubes de l’appareil. Utilisez uniquement desES

gentleMACS Tubes correctement installés avec des chapes étroitement fermées. N’utilisez pas l’appareil
seulement avec des chapes de gentleMACS Tube en place. Les tranchants exposés des chapes de gentleMACS
Tube peuvent provoquer des incisions, si ils sont touchés lorsqu’ils sont en rotation. Ne contournez aucuneFRmesure ou dispositif de sécurité.

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Dangers chimiques et biologiques
Selon la matière biologique utilisée, une contamination et infection peut entraîner des blessures corporelles
graves ou même la mort. Tout prélèvement clinique doit être considéré comme potentiellement contagieux.
Si des matières biologiquement dangereuses sont ou ont été utilisées, l’opérateur doit choisir etporter un équipement et des vêtements de protection, tel indiqué dans les avertissements et mesures desécurité pour la substance respective. La mesure de sécurité mentionnée ci-dessus s’applique également pour
toute substance chimique dangereuse, y compris toxique ou corrosive, acide ou radioactive, pouvant êtreprésente dans le prélèvement. Portez des gants et des vêtements de protection, ainsi que des lunettes
protectrices, pour que la substance n’entre pas en contact avec le visage et les yeux. Un équipement deprotection défectueux ou inadéquate est dangereux. L’appareil doit être utilisé dans une hotte de sécurité, si
des matières dangereuses ou inconnues sont traitées. Si des matières dangereuses ont été utilisées ou
renversées, l’appareil doit soigneusement et complètement être décontaminé. Il est strictement interdit decontinuer l’utilisation d’accessoires ou de pièces de l’appareil contaminées.
Des tubes et tout autre consommable qui étaient en contact avec des matières biologiquement dangereuses
doivent être décontaminés avant d’être jetés. Tout liquide et déchet solide doivent être considérés comme
dangereux et pour cela être traités selon les précautions universelles de laboratoire. L’enlèvement de déchets
doit être conforme aux réglementations locales.

Dangers de radiation optique
L’appareil est équipé de puissantes diodes électroluminescentes (DELs) pour illuminer le Safety Shield et les
gentleMACS Tubes. Selon la norme internationale CEI 62471, ce système de lampe a une valeur d’exposition
limite (ELV) de 0,91 et dépasse le groupe exempt de risques. La distance de sécurité (DS) pour le groupe
exempt de risques est de 61 cm. La distance de sécurité pour le groupe de risques 1 est de 20 cm. Le risque lié
au spectateur dépend de la façon dont est installée et utilisée l’appareil. La radiation optique émise de ces DELs

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peut être nuisible aux yeux à une proche distance de vue, si la Trough est enlevée. Débranchez l’appareil avantd’enlever la Trough. Si la Trough est enlevée, ne pas regarder dans l’éclairage des DELs à une distanceinférieure à 20 cm.
De plus, le lecteur de code 2D utilise des DELs pour illuminer la zone de lecture. Ne fixez pas directement lelecteur de code 2D des yeux à une courte distance, lorsqu’il fonctionne.
Ne démontez, modifiez, ou enlevez aucune des sources de radiation optique. Une radiation optique d’appareilsdémontés peut être nuisible aux yeux.

Surface chaude dangereuseEN

La surface des parties chauffantes du gentleMACS peut provoquer des brûlures en cas de contact. Ne touchez
pas la surface chauffante pendant le fonctionnement de l’appareil.

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Niveaux de bruits dangereux
Si l’appareil est utilisé à une charge maximale, il peut produire des bruits à un niveau dangereux. Des niveauxESde bruits dangereux peuvent entraîner une défaillance auditive. Il est recommandé que le corps responsable
mesure ou calcule le niveau de la pression acoustique à la position de l’opérateur. Il doit également définir àquel point à un mètre du boîtier du dispositif l’on mesure le plus haut niveau de pression acoustique. S’il n’estFR

pas garanti pendant l’installation que le niveau de pression acoustique de l’appareil n’atteindra pas une valeur
pouvant entraîner un danger à son endroit d’utilisation, des matériels de protection tels que des baffles ouhottes réduisant le bruit, ainsi que des protections sonores doivent être utilisées. Observez les réglementations

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locales en matière de protection auditive.

Maintenance, transport et élimination
N’entretenez pas vous-même l’appareil, à moins que ceci ait été spécifiquement mentionné dans ce manuelou d’autres documentations de Miltenyi Biotec,.
La maintenance et les réparations doivent être effectuées par un personnel de maintenance qualifié. Une
maintenance ou réparation impropre ou incorrecte de l’appareil peut causer des dangers pour l’utilisateur,amener des résultats imprévisibles, causer un dysfonctionnement ou une détérioration de l’appareil, ainsi
qu’une usure prématurée et une réduction de la longévité de l’appareil. Ceci peut également faire expirer votre
garantie.
Si des pièces de rechange sont nécessaires, assurez-vous que le prestataire de services utilise uniquement despièces authentiques de Miltenyi Biotec, ou des pièces de tiers spécifiées et recommandées par Miltenyi Biotec.
L’utilisation de pièces non autorisées peut entraîner un dysfonctionnement de l’appareil et impacter lesrésultats. Miltenyi Biotec n’accorde aucune garantie ou ne prend aucune responsabilité pour des défauts del’appareil ou des détériorations résultant de l’utilisation de pièces inappropriées. Après l’achèvement de tout
travail de maintenance et de réparation, assurez-vous que votre prestataire de services Miltenyi Biotec agréé
entreprenne tous les contrôles de sécurité, comme le demande la procédure de réparation pour garantir unfonctionnement normal de l’appareil.
Utilisez uniquement des options et extensions recommandées par Miltenyi Biotec. Renseignez-vous auprès devotre représentant local Miltenyi Biotec sur les arrangements extensives de support et de maintenance del’appareil, ou bien référez vous à www.miltenyibiotec.com/support.

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L’appareil doit être transporté avec soin dans des emballages spécifiés par Miltenyi Biotec. Une détérioration
intérieure peut se produire, si l’appareil fait l’objet de vibrations excessives ou d’une chute. Si l’appareil doitêtre expédié de retour au fabricant pour la maintenance, décontaminez l’appareil pour éliminer toute matière
dangereuse avant le chargement. Pour toute question concernant une décontamination ou un chargement
correct, veuillez contacter le support technique de Miltenyi Biotec.

Déchets d’Equipements Electriques et Electroniques (DEEE) – Information Client
Pensez à recycler vos produits Miltenyi Biotec en fin de vie en conformité avec la directive DEEE en vigueurdans votre pays.
Les équipements électriques et électroniques peuvent contenir des substances dangereuses, qui peuvent avoirEN

un effet néfaste sur l‘environnement et / ou la santé humaine. C‘est pourquoi tous les équipements doiventêtre spécifiquement collectés et traités par les centres désignés et ce en conformité avec laDE« Réglementation DEEE ». En vous assurant que vous éliminez vos équipements électriques et électroniques en
accord avec la législation en vigueur, vous contribuez à préserver nos ressources naturelles et à protéger laESsanté humaine.
La protection de l’environnement est au cœur de nos préoccupations. Ainsi, nous finançons de nombreux
programmes de collecte et de recyclage des équipements électriques que nous mettons sur le marché, enFRreversant une partie du prix de vente de nos produits à des éco-organismes agréés à travers le monde. Miltenyi
Biotec vous permet de recycler gratuitement vos équipements Miltenyi Biotec en fin de vie. Les conditions et laITdisponibilité de cette offre varient selon les pays et les différentes exigences réglementaires. Selon le type etl‘utilisation de votre équipement, des exigences supplémentaires peuvent s‘appliquer.
Pour plus d‘informations, ou si vous souhaitez recycler votre équipement Miltenyi Biotec en fin de vie,contactez votre interlocuteur Miltenyi Biotec ou notre support technique.

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INFORMATIONS DE SÉCURITÉ IMPORTANTESIMPORTANTI ISTRUZIONI DI SICUREZZAIT
Importanti istruzioni di sicurezzaEN

Il gentleMACS Octo Dissociator with Heaters è un apparecchio da laboratorio. Lo strumento può essere
utilizzato in sicurezza, se è garantito quanto segue: Lo strumento è stato installato correttamente. Lo strumentoè gestito solo da personale di laboratorio professionalmente. Vengono seguite le pratiche generali di sicurezza.

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Vengono seguite tutte le istruzioni contenute in questo manuale d´uso dell'utente.

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Avvisi e precauzioni
Le istruzioni in questa sezione spiegano i potenziali rischi associati all’uso di questo strumento e provvedono afornire delle informazioni di sicurezza aggiuntive per minimizzare il rischio. Si seguano le istruzioni

FRattentamente per proteggere Voi stessi, gli altri e l’attrezzatura da potenziali pericoli e creare un ambiente dilavoro sicuro. Si utilizzi questo strumento solo come specificato dal produttore per evitare danni al prodotto oITlesioni al personale.
Si seguano sempre le indicazioni di sicurezza specifiche del luogo di lavoro e le disposizioni del laboratorio,
nonché gli standard di salute, sicurezza e prevenzione incidenti. Si contatti l’autorità locale per il controllo delleforniture elettriche, le costruzioni della struttura, la manutenzione e la sicurezza, per ottenere maggiori
informazioni in merito all’installazione sicura e l’impiego dello strumento.

Livelli di pericolo
Segnali di avviso sono in uso per identificare un uso sicuro e messaggi in caso di danni a cose personali. I
seguenti segnali saranno in uso in questo manuale.
o WARNING! indica una situazione potenzialmente pericolosa, che, se non evitata, protrebbe causare morte ogravi danni.
o CAUTION! indica una situazione potenzialmente pericolosa, che, se non evitata, protrebbe causare danni
minori e lesioni moderate. Può essere impiegato per segnalare procedure pericolose.

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Simboli
I seguenti simboli sono impiegati per sottolineare condizioni che potrebbero causare lesioni a persone o danni
all´attrezzatura:
Avviso di sicurezza. Rischio di pericolo. Consultare il manuale di istruzioni ogni volta che questo simbolo diavvertimento viene utilizzato sul dispositivo, per identificare la natura del potenziale pericolo e stabilire qualiazioni intraprendere.
Rischio biologico. Rischio di contaminazione se è in uso materiale biologio potenzialmente pericoloso.
Rischio di scosse elettriche.

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Radiazione ottica pericolosa.

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Rischio di intrappolamento involontario delle dita a causa di parti rotanti.
Superficie calda pericolosa.

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Rischi di livello acustico.

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Morsetto di protezione. Questo simbolo è applicato internalmente allo strumento. Si tratta di informazioni per ilpersonale di servizio.
Fusibile.

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RAEEE (Rifiuti di apparecchiature elettriche ed elettroniche).

On (Alimentazione).
Off (Alimentazione).
Tutte le istruzioni operative devono essere lette prima di installare ed impiegare lo strumento.
Conformità valutata del regno unito

Approvazione di Conformità Europea
Conformità valutata del Regno Unito
Marcatura della certificazione NRTL: Il prodotto è conforme alle norme di sicurezza, basate sul consenso,
imposte dall’Occupational Safety/Health Administration (OSHA), determinate dai laboratori di prova nazionali
riconosciuti (Nationally Recognized Testing Laboratories (NRTL)) TÜV Süd

Etichetta di sicurezza
La seguente etichetta è applicata sul retro dello strumento e gioca un ruolo fondamentale nel mantenimentodel livello di sicurezza.
ATTENZIONE! Evitare lesioni gravi. Leggere e comprendere le istruzioni perl'uso prima dell'installazione o dell'uso. In caso di mancanza del manuale,richiedere la sostituzione a Miltenyi Biotec.

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IMPORTANTI ISTRUZIONI DI SICUREZZA

I seguenti simboli di sicurezza sono esposti sul fondo della vaschetta.

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Figure 1: Simbolo di sicurezza sul fondo della vaschetta del gentleMACS Octo Dissociator with Heaters.

Figure 2: Simbolo di sicurezza sul gentleMACS Heaters.

I perni sono evidenziati da una marcatura di sicurezza di colore diverso. La funzione delle marcature è quella dievidenziare il movimento rotatorio del perno.
Le etichette di sicurezza e i marchi di sicurezza devono essere mantenuti puliti e leggibili. Ispezionare
periodicamente le etichette di sicurezza e i marchi di sicurezza e sostituirli se illegibili o percettibili da una
distanza di sicurezza. Contattare Miltenyi Biotec per delle etichette sostitutive.

Istruzioni generali di sicurezza
Se lo strumento non funziona correttamente e/o le istruzioni visualizzate dei messaggi di avviso suggeriscono
di contattare l’assistenza tecnica, non è più possibile un uso sicuro dello strumento. Si spenga immediatamente
lo strumento e lo si scolleghi dalla presa di corrente, quindi si contatti il service provider Miltenyi Biotec o
Miltenyi Biotec Technical Support.

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Rischi elettrici e pericolo d'incendio
Possibili rischi derivanti da dispositivi elettrici includono scosse elettriche, corti e surriscaldamenti.
Una scossa elettrica potrebbe causare gravi lesioni personali o perfino la morte. Un corto elettrico o un
surriscardamento potrebbero causare un incendio.Le apparecchiature elettriche possono generare scintille,
che potrebbero incendiare vapori combustibili o materiali, causando esplosioni o incendi. Non si utilizzi lo
strumento in aree classificate come postazioni pericolose, p.e. in ambienti carichi di ossigeno.
Il corpo dello strumento é stato realizzato per ridurre il rischio di scosse elettriche, corti elettrici e la dispersione
di fuoco. Con eccezione per il Safety Shield e Trough, non si rimuova o si apra alcun rivestimento. Solo il
personale autorizzato può rimuovere le altre protezioni dello strumento. Non si inserisca mai alcun oggettolè aperto o smontato,lè caduto o danneggiato,lha danni o ha parti rotte,lha il cavo di alimentazione danneggiato,l

un oggetto è entrato nelle fessure di ventilazione,l

un oggetto esterno è stato inserito nello strumento.

ES

DE

EN

esterno nelle aperture dello strumento. Non si usi lo strumento se:FR

Se si vedono fiamme o fumo, si escluda immediatamente l’alimentazione, si scolleghi lo strumento dalla presadi corrente e si contatti un Miltenyi Biotec service provider autorizzato o Miltenyi Biotec Technical Support.

IT

Lo strumento è stato realizzato per un uso esclusivanente al coperto. Se dei fluidi entarno nello strumento,
potrebbero verificrsi dei corti eletrici, scosse elettriche o fuoco. Si faccia particolare attenzione quando si
ha a che fare con sostaze liquide.
Si faccia in modo che le stesse non fluiscano internamente all’apparecchio.
Si protegga l’apparecchio da fuoriuscite improvvise o getti d’acqua. Si puliscano immediatamente lefuoriuscite. Non si faccia uso dello strumento se delle sostanze liquide sono venute a contatto con lo stesso.
Non si usi l’apparecchio in un ambiente umido o con vapore. Si evitino aree con elevata umidità o rischio dicondensazione. Spostrare lo strumento da un ambiente freddo (come p.e. una stanza fredda a 5 °C) potrebbe
causare condensazioni interne allo stesso. In tal caso si aspetti che lo stesso si deumidifichi, prima di renderlo
nuovamente operativo.
Si stacchi lo strumento dalla presa elettrica prima di pulirlo. Non si usino liquidi o prodotti di pulizia aerosol; si
usi sempre un panno umido.
Si garantisca un adeguato ricircolo d’aria nella stanza dove è in uso lo strumento. Nel caso di assenza diun’adeguata circolazione, l’aria dell’ambiente circostante potrebbe non raffreddare lo strumento ad
un’acccettabile temperatura operativa. Si permetta una circolazione sufficiente attorno allo strumento –almeno 15 cm su tutti i lati –durante il funzionamento, per garantire un adeguato raffreddamento. Non si
posizioni lo strumento vicino a radiatori, fonti di calore, fornelli o qualsiasi altro apparecchio che generi calore(inclusi gli amplificatori). Non si coprano gli slots e le aperture dello strumento, poiché le stesse sono previste
per la ventilazione e per proteggere lo strumento da surriscaldamento. Non si posizioni lo strumento in uno
scomparto integrato o in un analogo spazio limitato, a meno che esso non si stato appositamente realizzato inmodo tale da garantire un’appropriata ventilazione. Si seguano le istruzioni di montaggio dello strumento.
Ci si assicuri che gli interruttori di rete principali, così come iI cavo di connessione alla corrente siano facilmente
accessibili e ubicati il più vicino possibile all’operatore. Se è necessario sconnettaere l’alimentazione, si
scolleghi il cavo dalla presa di corrente.

40

IMPORTANTI ISTRUZIONI DI SICUREZZA

Lo strumento è fornito di una spina con tre conduttori ed un terzo pin per il collegamento a terra. Questa spina
può essere collegata solamente con una presa di corrente a terra. Si tratta di una misura di sicurezza. Non si
provi ad inserire la spina in una presa non a terra. Nel caso non si riesca ad inserire la spina nella presa, si
contatti l’elettricista di fiducia per sostituirla.
Lo strumento dovrebbe venire alimentato solamente da una fonte di alimentazione che rispetti lespecificazioni menzionate nell’etichetta di indicazione elettrica del prodotto. Nel caso ci fossero domande in
merito al tipo di fontte energetica, si contatti il service provider Miltenyi Biotec autorizzato o la compagnia
energetica di fornitura locale. Non si usino prolunghe o prese multiple. Non si sovraccarichi una presa elettrica.

Rischi meccanici

EN

Spostare o far ruotare le parti potrebbe causare rischi di natura meccanica. Quando si fa uso dello strumento simantengano collegati i Trough, i Sleeves e i Safety Shield ad esso connessi. Mentre i rotori dello strumentoDEsono in funzione, non li si tocchi, non li si carichi o si rimuovano le provette dall´appaecchio. Si usino solamente
i gentleMACS Tubes, opportunamente installati con chiusure ermetiche. Non si faccia funzionare l´apparecchio
con solo i tappi dei gentleMACS Tubes in posizione. I bordi taglienti dei tappi dei gentleMACS Tubes

ES

potrebbero causare ferite, se toccati mentre in rotazione. Non si aggirino le misure di sicurezza o si usino
diversi dispositivi.

FR

Rischi chimici e biologici
In base al materiale biologico impiegato, alla contaminazione o all´infezione, possono verificarsi gravi lesioni aITpersone o anche la morte. Tutti i casi clinici devono essere considerati come potenzialmente infetti. Se un
materiale a rischio biologico è o è stato in uso, l´operatore deve scegliere ed indossare delle protezioni
di sicurezza personali come indicato negli avvisi e le precauzioni specifiche per la particolare sostanza. Le
precauzioni di sicurezza sopra indicate considerano qualsiasi altra sostanza chimica rischiosa, incluse sostanze
tossiche e corrosive, acide o radioattive che potrebbero essere presenti nel campione.
Si indossino guanti e abiti di sicurezza, nonché occhiali protettivi per prevenire il contatto tra la sostanza ed il
corpo e gli occhi. L´uso di abbigliamento protettivo danneggiato o inadeguatoo può essere pericoloso. Lo
strumento deve essere impiegato all´interno di un´area protetta nel caso vengano impiegati materiali
pericolosi o sconosciuti. Nel caso in cui del materiale percoloso sia stato utilizzato o sia fuoriuscito, si faccia
molta attenzione quando si sterilizzi (decontamini) lo strumento. E´severamente proibito continuare adutilizzare parti contaminate o altri accessori dello strumento.
Le provette o il materiale ausiliario entrati in contatto con materiali biologici pericolosi dovranno essere
sterilizzati prima dello smaltimento. Tutt i rifiuti liquidi e solidi dovranno essere considerati pericolosi e quindi
essere trattati secondo le precauzioni universali da laboratorio. Lo smaltimento dei rifiuti dovrà avvenire inaccordo con le normative locali vigenti.

Rischi di radiazioni ottiche
Lo strumento è fornito di un potente sistema di diodi luminosi (LEDs) per illuminare il Safety Shield ed igentleMACS Tubes. Secondo gli standard internazionali IEC 62471, questo sistema di illuminazione ha unvalore di rischio espositivo (EHV) di 0.91 ed è inoltre nel gruppo di rischio estremo (Exempt Risk Group). La
distanza di rischio (HD) per il Exempt Risk Group è di 61 cm. La distanza di rischio per il gruppo di rischio 1 è di
20 cm. Il rischio per gli astanti dipende da come lo strumento è installato e dalla modalità di funzionamento. La
radiazione ottica emessa dai LEDs può essere nociva per gli occhi a breve distanza, se il Trough viene rimosso.
Si sconnetta lo strumento prima di rimuovere il Trough. Una volta rimossa il Trough non si guardi in direzionedell´illuminazione dei LEDs, se non ad una distanza di almeno 20 cm.

41

IMPORTANTI ISTRUZIONI DI SICUREZZA

Anche il lettore di codice 2D integrato usa LEDs per l´illuminazione dell´area di lettura. Non si fissi direttamente
nel lettore di codice 2D a breve distanza, se questo è in funzione.
Non si smonti, si modifichi o si rimuova alcuna delle fonti di radiazione ottica.
La radiazione ottica degli strumenti smontati potrebbe essere nociva per gli occhi.

Rischio superficie calda
La lamina di riscaldamento del gentleMACS Heaters può diventare abbastanza calda da causare ustioni setoccata. Non toccare la lamina di riscaldamento mentre lo strumento è in funzione.

Rischi di livello acusticoEN

Se lo strumento è in uso con carico massimo, può generare rumore a livelli che possono essere pericolosi.
Rumori a livelli pericolosi potrebbero causare danni all´udito. Si raccomandano le misure indicateDE

dall´organismo responsabile o il calcolo dell livello della pressione sonora in prossimità della posizionedell´operatore ed a una qualunque posizione ad 1 m dalla zona dove si trova l´attrezzatura, che abbia il più alto
livello di pressione sonora. Se non viene verificato durante l´installazione che il livello di pressione del suonoESdal dispositivo non raggiunga un valore tale durante il suo uso, che possa causare dei rischi, allora deve essere
impiegato del materiale protettivo come pannelli acustici, cappe o tappi per le orecchie. Osservare le normeFR

locali sulla protezione dell'udito.

Manutenzione, trasporto e smaltimento
IT

A meno che non differentemente specificato in questo manuale o in altra documentazione Miltenyi Biotec,
non si faccia la manutenzione dello strumento da soli. La manutezione e le riparazione devono essere
condotte da personale di servizio qualificato. Una manutenzione impropria o scorretta, ovvero riparazioni dello
strumento possono causare pericolo per gli utenti o portare a danni irreparabili, causare un malfunzionamentodello strumento o danni, nonchè usura prematura e ridotta vita utile dello stesso. Potrebbe inoltre fare caderela Vostra garanzia.
Sostituendo componenti o se sono necessari pezzi di ricambio, assicurarsi che il service provider usi solo parti
originali Miltenyi Biotec o altrimenti terze parti consigliate e raccomandate da Miltenyi Biotec. L´utilizzo di parti
non originali può causare malfunzionamenti dello strumento e risultati imprecisi. Miltenyi Biotec non offre
alcuna garanzia nè accetta alcuna responsabilità per danni alla strumentazione o guasti dovuti ad un uso
sconsiderato del componenti. Dopo aver completato ogni servizio di manutenzione e lavoro di riparazione, ci si
assicuri che il service provider Miltenyi Biotec autorizzato esegua tutti i controlli di sicurezza previsti dalle
procedure, per garantire che lo strumento funzioni in modo corretto.
Si usino solo opzioni ed aggiornamenti raccomandati da Miltenyi Biotec.
Si chiedano informazioni al rappresentante locale di Miltenyi Biotec in merito al servizio esteso di strumenti
Miltenyi Biotec’s e le modalità di supporto, o ci si riferisca a www.miltenyibiotec.com/support.
Lo strumento dovrebbe essere trasportato con cura nell´imballo specificato da Miltenyi Biotec. Danni interni
possono essere provocati da eccessive vibrazioni o se lo strumento dovesse cadere. Nel caso l´apparecchio
debba essere spedito al produttore, lo si sterilizzi per eliminare ogni sostanza pericolosa prima dellaspedizione. Nel caso ci fossero domande in merito all´adeguata pulizia o la spedizione, si contatti perl´assisenza il Miltenyi Biotec Technical Support.

Informazioni per i clienti riguardo lo smaltimento delle apparecchiature elettriche ed elettroniche
secondo le normative WEEE (Waste of Electrical and Electronic Equipment)

42

IMPORTANTI ISTRUZIONI DI SICUREZZASi prega di smaltire i prodotti Miltenyi Biotec in disuso secondo le disposizioni di legge riguardanti losmaltimento di apparecchiature elettriche ed elettroniche (WEEE) e dei rifiuti pericolosi, che possono differireda paese a paese.
Le apparecchiature elettriche ed elettroniche possono contenere sostanze pericolose, che possono avere un
grave effetto dannoso per l’ambiente e / o la salute umana. È per questo che tutte le attrezzature devono
essere specificamente raccolte e trattati da centri di smaltimento abilitati e qualificati in conformità con lanormativa WEEE. Il corretto smaltimento delle apparecchiature elettriche ed elettroniche secondo ledisposizioni di legge WEEE e dei rifiuti pericolosi, possono aiutare a preservare le risorse naturali e a tutelare lasalute umana.
Miltenyi Biotec si impegna a proteggere l’ambiente. Miltenyi Biotec offre programmi per lo smaltimento dei

ENprodotti dismessi in molti paesi e partner accreditati per lo smaltimento secondo le normative WEEE in tutto il
mondo. Miltenyi Biotec consente di riciclare le apparecchiature Miltenyi Biotec gratuitamente. Le condizioni eDEla disponibilità di questa offerta variano da zona a zona a causa di differenze legislative. Si prega di notare che,a seconda del tipo e dell’utilizzo delle apparecchiature, possono essere necessari requisiti aggiuntivi.

ES

Per ulteriori informazioni, o se si desiderassero smaltire le apparecchiature Miltenyi Biotec in disuso, si prega diITFRcontattare il Responsabile di Zona o il Supporto Tecnico Miltenyi Biotec.

43

44

IT
FR

ES

DE

EN

IMPORTANTI ISTRUZIONI DI SICUREZZA1
INTRODUCTION

Introduction1

1.1

Preparation of single-cell suspensions and homogenates from tissues
The gentleMACS Octo Dissociator with Heaters provides the perfect solution for the fast and easy preparationof single-cell suspensions and homogenates from virtually any tissue. Samples are processed using uniquegentleMACS Tubes with caps that have specially designed rotors and stators. C Tubes provide gentle but
efficient tissue dissociation for cell suspensions with high viability rate. M Tubes provide thoroughhomogenization of tissues or cells.

Figure 1.1: The gentleMACS Octo Dissociator with Heaters equipped with gentleMACS Tubes and Heaters.

The gentleMACS Octo Dissociator with Heaters provides:
l

l

1.2

the preparation of viable single-cell suspensions by mechanical and enzymatic dissociation, including incubations
at 37 °C,
the preparation of thorough tissue homogenates for subsequent extraction of sub-cellular material, such as total
RNA, DNA, proteins, organelles, and pathogens,lsimultaneous or independent processing of up to eight samples, even using different programs,leasy access to pre-defined gentleMACS Programs which are optimized for particular applications,lthe option to create user-defined programs.

Sample preparation for cell and molecular biology applications
Two dedicated types of gentleMACS Tubes have been specially designed for the optimal preparation ofsamples for cell biology or molecular biology applications:45l

INTRODUCTIONl

Purple C Tubes are typically used for the gentle preparation of single-cell suspensions from tissues for subsequentcell separation, cell analysis, or cell culture.
Orange M Tubes are typically used for the homogenization of tissues and cells for subsequent subcellular or
molecular analyses.

gentleMACS Tubes allow for:lthe processing of sample volumes from 300 μL to 10 mL and from 20 mg to 4,000 mg of tissue in a single tube,

lsterile sample handling.

gentleMACS Tubes consist of a stator and rotor element, that provides the exact sheer forces necessary to

1gently pull cells from tissues or intact molecules from cells. A specially engineered tube enclosure always
directs the sample flow towards the rotor and stator element. This sophisticated design results in highly
efficient tissue dissociation and homogenization using C Tubes and M Tubes, respectively.

Figure 1.2: Caps of the gentleMACS Tubes (exemplary, M Tubes: orange, C Tubes: purple).

1.3

Sample preparation combining enzymatic and mechanical dissociation
For combined enzymatic and mechanical dissociation processes the instrument comes equipped withgentleMACS Heaters for each of the eight tube positions. The gentleMACS Heaters allow to apply 37 °C either
to all eight positions or individually to each tube. By gently pressing the clamps, the gentleMACS Heaters canbe easily attached to the tube positions, thus connecting the gentleMACS Heaters to the instrument via its
electrical contact pins.

Figure 1.3: The gentleMACS Heaters for incubation of samples at 37 °C.

46

1.4

MACS Tissue Dissociation Kits
A broad selection of optimized and easy-to-use MACS Tissue Dissociation Kits, including pre-defined enzyme
mixes, allows for convenient and reproducible tissue dissociation using the gentleMACS Octo Dissociator with
INTRODUCTION

Heaters. A comprehensive list of MACS Tissue Dissociation Kits for numerous applications is available fordownload at www.gentlemacs.com.

1.5

Pre-defined tissue dissociation and homogenization programsMore than 40 pre-set gentleMACS Programs without heating step and including an incubation step at 37 °C
have been specifically developed to offer standardized procedures for tissue dissociation in virtually any

1research field. A comprehensive list of optimized gentleMACS Programs and detailed gentleMACS Protocols fornumerous applications are available for download at www.gentlemacs.com.

1.6

Customized dissociation programs
In addition to the ready-to-use gentleMACS Programs optimized for specific applications, the gentleMACS Octo
Dissociator with Heaters also allows users to create new programs. This can be accomplished afresh or bymodifying pre-defined templates.

1.7

Intended use
The gentleMACS Octo Dissociator with Heaters is a benchtop instrument for the fully automated andstandardized tissue dissociation or homogenization of up to eight samples.
Equipped with eight individual heating units and numerous optimized and ready-to-use gentleMACS Programs
the gentleMACS Octo Dissociator with Heaters offers a fully automated workflow for tissue dissociation. In
addition the instrument allows the user to create user-defined programs for almost any biological material. Alleight positions can be operated independently.
Single-cell suspensions or thorough homogenates are easily and reproducibly obtained using the unique C
Tubes or M Tubes. These single-use gentleMACS Tubes allow sample preparation in a closed and sterile system,
providing a high level of user safety and minimize cross-contamination.
The gentleMACS Octo Dissociator with Heaters is for research-use only in laboratory environment. It is to be
used by laboratory professional users only.

47

1

48

INTRODUCTION

2
INSTALLATION

Installation

Please unpack the instrument carefully and confirm that all components have been successfully delivered
without damage that may have occurred during transportation.

2

WARNING! If there is any damage, do not use the instrument. Contact your local Miltenyi Biotec representativeor Miltenyi Biotec Technical Support. Operating a damaged instrument may cause mechanical hazards, opticalradiation hazards, fire hazards, or electric shock. Read the chapter Important safety information before
installing and assembling the instrument.
Please retain the original packaging material.

2.1

Components included in the delivery
The following components are included in the delivery of the gentleMACS Octo Dissociator with Heaters(# 130-096-427):
1× gentleMACS Octo Dissociator with Heaters
8× gentleMACS Heaters
1× Power cord
1× User manual

2.2

Installation
Read the chapter Important safety information and ensure that the site is properly prepared before
continuing with installation and assembly. Incorrect installation can lead to the spread of fire, explosion, therisk of electric shock, or mechanical hazards.
Note: The gentleMACS Octo Dissociator with Heaters is a ready-to-use instrument that requires a power
connection and a flat surface of the dimensions 465×285 mm plus at least 15 cm on all sides to allow
sufficient air circulation. To operate the instrument, the display and the top of the instrument must beaccessible.
To install the gentleMACS Octo Dissociator with Heaters:
1. Carefully remove the device from the cardboard box.
2. Place the instrument on a secure and stable workbench that can support its weight and size. Ensure that the
instrument is not exposed to direct sunlight.

CAUTION! Do not place the instrument on an unstable table, cart, stand, tripod, or bracket. As aconsequence, the instrument might fall down. This may cause serious bodily harm and/or serious damage
to the instrument. Use only on a table, cart, stand, tripod, or bracket recommended by Miltenyi Biotec.

49

3. Plug the instrument into the power mains.

WARNING! The instrument is equipped with a three-wire electrical groundingtype plug. This is a safety
feature to reduce the risk of electric shock. Do not insert the plug into a non-grounded power outlet. If you
cannot insert the plug into the outlet or if you are not sure whether the outlet is grounded, contact yourlocal electrician.

INSTALLATION

The instrument should only be operated from a power source whose specifications match those indicatedon the instrument’s electrical ratings label. If you have questions about the type of power source to use,
contact your authorized Miltenyi Biotec service provider or local power company. Ensure that the mains
switch as well as the connector for the power cable are easily accessible and located as close to the operatorof the instrument as possible. If it is necessary to disconnect the instrument’s power supply, unplug thecable from the power outlet.

2
4. Set the switch located above the plug socket to ON (“I” indicates “ON”, “O” indicates “OFF”).
5. The instrument will perform a start-up check that runs for a few seconds.

50

3

3.1

MATERIALS REQUIRED

Materials required

Materials required to operate the instrument
The following materials are required to operate the gentleMACS Octo Dissociator with Heaters.

Product

Description

Capacity

Order no.

C Tubes

For gentle preparation of singlecell suspensions from tissues for
subsequent cell separation, cell
analysis, and cell culture.

25 tubes, sterile, single-packed

130-093-237

100 tubes, sterile, packed as
4×25 tubes

130-096-334

For homogenization of tissues
and cells for subsequent
subcellular and molecularanalysis.

25 tubes, sterile, single-packed

130-093-236

100 tubes, sterile, packed as
4×25 tubes

130-096-335

For homogenization of tissues
and cells for subsequent
subcellular and molecularanalysis. The strainer removes
aggregates or large particles
from the sample.

50 tubes per bag, sterile

130-094-392

M Tubes

M Tubes with
Strainer3(strainer: 600 μm mesh)

Table 3.1: Disposables required for the gentleMACS Octo Dissociator with Heaters.

MACS SmartStrainers are filters that are designed for the easy removal of cell aggregates or large particles
after tissue dissociation. The SmartStrainers are available with three mesh sizes: 30 μm (50 pieces # 130-098458, 4×25 pieces # 130-110-915), 70 μm (50 pieces # 130-098-462, 4×25 pieces # 130-110-916), and 100 μm (50pieces # 130-098-463, 4×25 pieces #130-110-917). They easily fit into standard 15 and 50 mL conical tubes of all
major suppliers. Strainers are stackable and thus allow filtration with decreasing mesh sizes in one go.
MACS Tissue Dissociation Kits were specifically designed for the convenient and standardized enzymatic
dissociation of multiple tissues in combination with the gentleMACS Octo Dissociator with Heaters. For acomprehensive list of MACS Tissue Dissociation Kits, please refer to www.gentlemacs.com.

3.2

Spare parts
Product

Description

Order no.

gentleMACS Sleeves

Adapters for attaching the gentleMACS Tubes

130-093-464

gentleMACS Heaters

Heating units

130-105-228

gentleMACS Octo TroughSafety container for spilled liquid

130-096-541

gentleMACS Octo Safety Shield

Safety shield for protection

130-096-542

Table 3.2: Spare parts of the gentleMACS Octo Dissociator with Heaters51

Note: Only use spare parts and accessories recommended by Miltenyi Biotec. Failure to use recommended
spare parts and disposables may compromise safe operation and may result in inaccurate results and reduced
life-time of the instrument. Miltenyi Biotec does not honor any warranty or accept any responsibility for

MATERIALS REQUIRED

damages resulting from the use of inappropriate accessories.

3

52

4
INSTRUMENT OPERATION

Instrument operation

Read the instructions in the chapter Important safety information before operating the instrument.
l

l

l

l

l

4.1

When processing infectious, radioactive, poisonous, or any other hazardous liquids, always take the necessary
safety precautions.
Before using organic solvents or aggressive chemicals in the dissociation or homogenization process, thecompatibility with the instrument’s parts and tubes must be tested.
Do not operate the instrument if the Sleeves, the Trough, or the Safety Shield are removed. For instrument
schematics, refer to section 4.2.

4

Do not leave the gentleMACS Heaters attached to the tube position while not in use to avoid wearing of the
gentleMACS Heaters.
Do not switch off the instrument while running a program to avoid severe damage of the instrument.

General information for operating the instrument
The gentleMACS Octo Dissociator with Heaters is used as a stand-alone, benchtop instrument. A number of
pre-defined programs offer a variety of optimized protocols for the automated dissociation andhomogenization of tissues. The instrument also allows creation and use of user-defined programs for specificapplications.
gentleMACS C or M Tubes are required for processing the samples. The gentleMACS Octo Dissociator with
Heaters must be used exclusively with these gentleMACS Tubes. Moreover, the gentleMACS Heaters are
specially designed for use with the instrument, when running a program that includes an incubation step.

4.2

Instrument schematics

Figure 4.1: Front of the gentleMACS Octo Dissociator with Heaters.

53

INSTRUMENT OPERATION

Figure 4.2: Back of the gentleMACS Octo Dissociator with Heaters.

4

Figure 4.3: Back of the gentleMACS Octo Dissociator with Heaters, Safety Shield removed.

4.3

Starting the Instrument
To start the instrument:
1. Ensure that the device is plugged in before setting the switch to the ON position (“I” indicates “ON”, “O” indicates“OFF”).
2. The instrument starts within a few seconds and displays the Main menu.

Note: Do not switch off the instrument while running a program to avoid severe damage of the instrument!
The gentleMACS Octo Dissociator with Heaters is operated using the touchscreen display. Items that can beselected are displayed in yellow, while items that cannot be selected are displayed in white.

54

Buttons and symbols:
Buttons

Function

Description

scroll up

The scroll buttons are used to scroll up or down, e.g., to highlight a gentleMACS

scroll down

Info button

Program in the Main menu tab, or device settings in the Setup tab.

A pop-up screen is displayed when pressing on the Info button, and displays
INSTRUMENT OPERATION

Icon

information about the highlighted gentleMACS Program, including program name,selected tube type, date of program creation, duration of the program, rounds perrun (rpr), and temperature (°C) applied.
Table 4.1: Buttons of the gentleMACS Octo Dissociator with Heaters display.

Folders
Different folders can be accessed by pressing a folder icon. The arrow indicates scroll function.

Icon

FolderDescription

Miltenyi

The Miltenyi folder contains gentleMACS Programs provided by Miltenyi

4

Biotec. The lock on the Miltenyi folder indicates that programs in this folder
cannot be edited or deleted.
Templates

The Templates folder contains gentleMACS Programs provided by Miltenyi
Biotec. Programs in this folder can be edited but must be renamed andsaved in a User folder.

User - 1

Five User folders are available to manage user-defined programs.

User - 2

User - 3User - 4

User - 5Favorites

Links of programs in the Favorites folder enable quick access.

USB stick

A USB stick can be installed to transfer programs to and from a folder.

Table 4.2: Overview of folders and respective icons.

55

LEDs
Various statuses of the instrument are indicated by LEDs illuminating the Safety Shield and the gentleMACS

INSTRUMENT OPERATION

Tubes.

Color

LED mode

Main menu

Green

SteadyStatus of one or more positions is “Free”. Note that Editor and Setup menus are
illuminated by a steady green light.

Blue

SteadyStatus of all eight positions is “Running”.

Blue

Flashing

Status of one or more positions is “Done”.

Red

Flashing

Error occurred on one or more positions.

Table 4.3: Overview of LED colors, modes, and status.

4.3.1

Menu tabs
The gentleMACS Octo Dissociator with Heaters is controlled using three menus present on the touchscreen of
the instrument.

4

Main menu
The Main menu displays the availability and status of all eight gentleMACS Tube positions. Each tube position
is diagrammatically shown as a square panel at the top of the screen. In this user manual, each panel is referred
to as a tube position.

Figure 4.4: Main menu of the gentleMACS Octo Dissociator with Heaters.

The Main menu is used to assign gentleMACS Programs to each tube position and run the respective process.
Programs can be loaded from a total of eight folders, a Favorites folder, a Miltenyi program folder, aTemplates folder, and five User folders. Please refer to section 4.4 for more details.
Editor menu
The Editor menu is used to create and edit programs. Moreover, programs are managed here. Links toprograms can be added to the Favorites folder and programs can be transferred among folders. Please refer tosection 4.5 for more details.

56

INSTRUMENT OPERATION

Figure 4.5: Editor menu of the gentleMACS Octo Dissociator with Heaters.

Setup menu
Instrument settings can be modified using the Setup menu. This includes switching the LEDs on or off, setting
the time and date, and naming User folders. Moreover this menu provides information on software andprogram versions. Please refer to section 4.6 for more details.

4

Figure 4.6: Setup menu of the gentleMACS Octo Dissociator with Heaters.

4.4

Main menu
The Main menu is used to select and run dissociation programs for each of the eight tube positions. Users
have the flexibility to run multiple programs simultaneously or sequentially. Samples can also be run
individually. The Main menu also reports the status of each tube position.

57

4.4.1

Status of sample tube positions

INSTRUMENT OPERATION

All possible statuses of tube positions are shown in 4.4.1 and described inTable 4.4.

Figure 4.7: Overview of tube position statuses displayed in the Main menu.

4

Tube position

StatusDescription

1

Free

No tube is inserted. The position is free to use.

2

Selected

Tube is inserted. After inserting a tube, the position is
automatically assigned the status “Selected”. Programs
can only be designated to positions with the status“Selected”.

3

Ready and Heater installed

The tube position is ready. A tube and a heater wereinserted and a program was subsequently assigned byconfirming the selection with “OK”. The program may becommenced by pressing “Start”. The program name andduration are displayed. In this example, program ”37°C_
m_tumor_01” will be completed within 5 minutes.

4

Ready

The tube position is ready. A tube was inserted and a
program was subsequently assigned by confirming theselection with “OK”. The program may be commenced bypressing “Start”. The program name and duration aredisplayed. In this example, program” m_neoheart_01_01”
will be completed within 1 minute.

5

Running

The program “m_ neoheart_01_01” is running. The
progress bar and timer display the time remaining tocomplete the program, in this example, 15 seconds.

6

Done

The program “m_ neoheart_01_01” is finished. The timer
displays the elapsed time since completion of the
program, in this example, 3 seconds.

7

AbortedThe program was stopped. It is possible to abort any
program by pressing the required tube position on thetouchscreen. The timer displays the remaining time tocomplete the program since abortion, in this example, 58
seconds.

8

Stand by

The tube position was deselected. Inserted tubes areautomatically assigned the status “Selected”. Deselectionof an inserted tube is achieved by pressing the required
tube position on the touchscreen. The status of the tube
position will change to “Stand by”. Note that programs
cannot be assigned to tube positions in the Stand bymode.

Table 4.4: Overview of tube position statuses.

58

Processing samples
Tips and hints for the processing of samples:l

The tubes are for single use only, (single procedure).

l

Only use C Tubes with the gentleMACS Heaters.

lSample sizes range from 300 μL to maximal 10 mL total volume and 20 to 4,000 mg of tissue.

l

l

l

The maximal amount of tissue depends on the consistency of the tissue as well as on the respective gentleMACS
Protocol.
Dependent on the stiffness of the tissue it might be recommended to cut the sample into smaller pieces before
processing on the gentleMACS Octo Dissociator with Heaters.

INSTRUMENT OPERATION

4.4.2

The tubes are suitable for a broad range of tissues, for example, spleen, liver, or brain. However, very hardmaterial, such as bone, may damage the tubes.

For further details, refer to the respective gentleMACS Tube data sheets and to the gentleMACS Protocols at
www.gentlemacs.com.
The following instructions assume that the instrument was successfully installed and initiated as outlined in
sections 2.2 and 4.3.

4

To process samples, transfer an appropriate amount of tissue and buffer or other appropriate fluid into agentleMACS C Tube or M Tube.
Note: Tightly close the tube beyond the first resistance.

Running a single sample
Note: Do not switch off the instrument while running a program to avoid severe damage of the instrument.
1. Available tube positions are indicated as Free.

2. Attach the tube upside down to the Sleeve of an available tube position. The instrument will automatically detect
the inserted tube and assign the tube status Selected.

59

INSTRUMENT OPERATION

Note: Please make sure that the tube is inserted and removed in an upright position without tilting. Tilting thetube at an angle might result in damage of the tube seal and subsequent leakage.
Note: Ensure that the tube is securely fastened to its sleeve and socket. It should ‘click’ into position. Do not force
the tube into the position. In case of resistance slightly turn the tube clockwise in order to find the right position
to click in.

4

3. (Optional) If running a program including an incubation step, apply a gentleMACS Heater to the respective tube
position. Slightly open the clamp of the gentleMACS Heater by pressing the upper handles and adjust it over the
tube. Make sure the lower side of the gentleMACS Heater is facing towards you therefore allowing the electricalcontact to close tightly. The instrument will automatically detect the attached gentleMACS Heater.
Note: Make sure that the gentleMACS Heater is securely fastened around the tube and to the socket. Do not use
the gentleMACS Heater without a tube.
4. To select a dissociation program, press the Folder icon to select the required folder.
Note: Programs can be selected from a total of eight folders: Favorites, Miltenyi, Templates, and five User
folders. Press the folder icon several times to scroll through.
5. The list of gentleMACS Programs in the respective folder will be displayed.
6. Highlight the required gentleMACS Program touching the screen or using the scroll buttons.
Note: Press the column header Programs to sort programs within a folder alphabetically. Press the column
header Date to sort programs by the date of creation.
7. Select the required program and press OK to confirm. The status of the tube position will change to Ready. The
timer indicates the duration of the program.

Note: Use the Clear button to reset the position from the Ready status into the Selected status to assign a newprogram.
8. Press Start to commence the program. The status of the tube position will change to Running. The progress bar
and timer indicate the time remaining.
Note: After selecting the program it is possible to skip the confirmation step OK by immediately pressing Start.

60

INSTRUMENT OPERATION

The program will immediately start, and the status of the tube position will change to Running.

9. On completion of the program, the status of the tube position will change to Done. The progress bar and timer
display the elapsed time since completion of the program.

4

Note: For further processing, use the Clear button to reset the position from the Done status into the Selected
status to assign a new program.
10. Remove the tube from the tube position. The status of the tube position will change to Free.

Running multiple samples simultaneously
Up to eight samples can be run simultaneously using the same or different programs. In this example, eightsamples will be processed using the same program.
Note: Do not switch off the instrument while running a program to avoid severe damage of the instrument!
1. Attach the tubes upside down to the Sleeves of the free tube positions. The status of the tube positions willchange from Free to Selected.

2. To select a dissociation program, press the Folder icon to select the required folder. The list of gentleMACS
Programs in the respective folder will be displayed.

61

Note: Programs can be selected from a total of eight folders: Favorites, Miltenyi, Templates, and five User
folders. Press the folder icon several times to scroll through.
3. Highlight the desired gentleMACS Program touching the screen or using the scroll buttons.
Note: Press the column header Programs to sort programs within a folder alphabetically. Press the column
header Date to sort programs by the date of creation.

INSTRUMENT OPERATION

4. Select the required program and press OK to confirm. The program will be assigned to all eight tube positions.
The status of the tube positions will change to Ready.

4
Note: Use the Clear button to reset positions from the Ready status into the Selected status to assign new
programs.
5. Press Start to commence the program. The status of the tube positions will change to Running. The progress bar
and timer indicate the time remaining.
Note: After selecting the program it is possible to skip the confirmation step OK by immediately pressing Start.
The program will immediately start, and the status of the tube positions will change to Running.

6. On completion of the program, the status of the tube positions will change to Done. The progress bar and timer
display the elapsed time since completion of the program.

Note: For further processing, use the Clear button to reset all positions from the Done status into the Selected62status to assign new programs.
7. Remove the tubes from the tube positions. The status of the tube positions will change to Free

Running multiple samples sequentially
Multiple samples can be run sequentially. In this example, three samples are processed using three different
programs.

llSample A is processed with program m_brain_01_01:
a Miltenyi Biotec program in the Miltenyi folder, optimized for the preparation of single-cell suspensions frommouse neural tissue.

INSTRUMENT OPERATIONl

Sample B is processed with program 37°C_m_tumor_01:
a Miltenyi Biotec program in the Miltenyi folder, optimized for the preparation of single–cell suspensions frommouse tumor, including an incubation step.
Sample C is processed with program C_01:
a template program provided by Miltenyi Biotec in the Templates folder. Programs in the Templates folder canalso be used as a basis to develop customized programs.

Note: Do not switch off the instrument while running a program to avoid severe damage of the instrument!

4

1. Insert Sample A into a free position. The status of the tube position will change from Free to Selected.
2. Press the Folder icon to locate the Miltenyi folder.

3. Select the program m_brain_01_01 from the list and press OK to confirm. The status of the tube position willchange to Ready.

4. Press Start to commence the program. The status of the tube position will change to Running.
Note: After selecting the program it is possible to skip the confirmation step OK by immediately pressing Start.
The program will immediately start, and the status of the tube position will change to Running.

63

INSTRUMENT OPERATION

5. While Sample A is still running, insert Sample B into a free position, attach a gentleMACS Heater to the selected
position as described in section 4.4.2.1 and select the program 37°C_m_tumor_01 as described in steps 2 and 3.
6. Press OK and Start to commence processing of Sample B.

4

7. While Sample A and Sample B are running, insert Sample C into the next free position and select the program
C_01 from the Templates folder. Press OK and Start to commence processing of Sample C.

8. Remove the samples when the status of the tube positions changes to Done. In this example, Sample A andSample C can be removed while Sample B is still running.

64

4.5

Editor menu
The Editor menu is used tolcreate new programs (for details, refer to section 4.5.1),

ledit programs (for details, refer to section 4.5.2),

lcreate program shortcuts into the Favorites folder (for details, refer to section 4.5.3),ltransfer programs between folders and to a USB stick and vice versa (for details, refer to section 4.5.4).

INSTRUMENT OPERATION

Note: Programs from the Miltenyi folder are not editable. Miltenyi templates from the Templates folder canbe edited but must be renamed and saved in one of the User folders.

4.5.1

Creating a new program
There are several optional commands to design a user-defined program, including options on the temperature,
rotation speed, direction, and duration, acceleration, and iteration. An overview of program commands isshown in Table 4.5.
Tips and hints for user-defined programs:

4
l

l

l

Do not exceed a maximum of 3,200 rounds per run, when creating a new program. Running higher numbers ofrounds per run may result in damage of the sealing of the gentleMACS Tube.
Typically, gentleMACS Programs consist of several spin commands, each with a different speed and clockwise
rotation. Integration of short counterclockwise spin commands can increase the efficiency of the dissociation or
homogenization.
Incubation periods typically run at –20 rpm. A counter-clockwise rotation has been beneficial in many cases.

Command

Description

temp ON

Defines the beginning of an incubation period. Any command between the temp ON andtemp OFF command will be performed at 37 °C.

temp OFFDefines the end of an incubation period. This command must be entered to stop the heating
function.

spin

Defines the speed, direction, and duration of rotation. Speed is defined as revolutions per
minute (rpm). Prefixing the rpm value with minus (–) results in a counter-clockwise rotation.
The total duration is given in minutes and seconds.
The following are the speed restrictions:
Clockwise: +20 to +4,000 rpm
Counter-clockwise: –20 to –4,000 rpm
The change of velocity must not exceed 6,000 rpm per second.

ramp

Defines the time required to reach a certain speed. This is given in revolutions per minute(rpm).
If a program starts with a ramp command, the starting speed will be 0 rpm.
If a ramp command is added after a spin command, the starting speed will be from the rpm of
the spin command.

loop

Defines the beginning of a loop cycle.
Commands between the loop and end loop commands are repeatedly executed for thedefined number of cycles. A maximum of 200 cycles can be defined.

end loop

Defines the end of a loop cycle. This command must be entered as the last step of a loop
cycle.

Table 4.5: Overview of program commands.

65

Creation of a user-defined program

INSTRUMENT OPERATION

1. Highlight a particular User folder. In this example, the folder User_1 is selected.

2. Press Add. A new_program file template will be displayed.
3. Press the tube button to select a C Tube or M Tube.
4. To select further options, e.g., spin, ramp, temp, loop, and endloop press Add. A dotted line will appear above
the end command.

4

Figure 4.8: New_program file template to create user-defined program.

5. Enter respective values and press OK.
6. Press Add to insert the next command or Save to name and store the program.

Example of an user-defined program
1. ramp 2,000 rpm, 10’’
2. loop 3×
3. spin –2,000 rpm, 10’’
4. spin 2,000 rpm, 10’’
5. end loop
6. temp ON
7. spin –20 rpm, 30’
8. spin 3,000 rpm, 10’’
9. temp OFF
10. end66

INSTRUMENT OPERATION

Figure 4.9: Graph of the example user-defined program.

Note: It is possible to insert or edit a command at any step above the end command. A new command will beinserted above a highlighted command. Commands can be highlighted using the touchscreen or the scroll
buttons.
Note: Highlighted commands, except for the end command, can be deleted by pressing Delete.

4
1. Press spin. Enter all parameters for the selected command using the pop-up keypad. Enter speed (rpm) and time(min and sec).

2. Press OK to confirm a value or Exit to cancel.
Parameters for an individual step will be displayed in an abbreviated form. In this example, spin –2,000 rpm, 1’’
indicates a counter-clockwise spin of 2,000 rpm for 1 second.

67

INSTRUMENT OPERATION

3. Press Add to insert the next command or Save to name and store the program for future use (refer to step 11.). In
this example, a second spin command is added, namely a clockwise spin at 2,000 rpm for 10 seconds.

Note: Loop commands enable the repetition of program steps. The iterations will only include the steps between
a loop and end loop command. In this example, the two spin commands shall be repeated three times.
4. Highlight the first command and press Add to select the next command. Select loop to mark the beginning of an
iteration. Enter the number of iterative cycles. In this example, 3 cycles are chosen.

4

5. Click OK to confirm the selection. The command loop and the number of cycles are displayed as a new step.
6. Highlight the end command and press Add. Select end loop to close the iterative cycle.

7. Press OK to confirm.
8. Highlight the first command and press Add to insert another command. Select ramp to reach a certain speed in adefined time.

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INSTRUMENT OPERATION

9. Enter speed and time. Press OK to confirm the values. In this example, a ramp of 2,000 rpm in 10 seconds has
been added.

10. Highlight the end command and press Add. Select temp and ON from the pop-up screen to mark the beginningof the heating period. Press OK to save the value.

4

Note: The temp command enables the heating of a sample to 37 °C during a series of program steps, e.g., forincubation purposes. The incubation period will only include the steps between a temp ON and temp OFF
command.
11. Highlight the end command and press Add. In this example, two spin commands are added, a counter-clockwise
spin at 20 rpm for 30 minutes and a clockwise spin at 3,000 rpm for 10 seconds.

69

INSTRUMENT OPERATION

12. Highlight the end command and press Add. Select temp and OFF from the pop-up screen to switch off theheating function. Press OK to save the value.

13. Press Save to store the new program. Enter the filename when prompted. In this example, the new program isnamed program_03.

4

Note: Program names can be a maximum of 16 characters. Select CAP to alternate between upper- and lowercase characters.
14. Select OK on the keypad to save the program. The program will be automatically saved to the folder selected instep 1. In this example, this is the folder User_1.

Note: The duration of a gentleMACS Program is limited to 3,200 rounds per run. For example, a program thatconsists of one spin command at 4,000 rpm can run for a maximum of 48 seconds; a program that consists of
one spin command at 200 rpm can run for a maximum of 960 seconds (16 minutes). A program that consists of
one spin command at 20 rpm can run for a maximum of 160 minutes.

70

It is not recommended to run programs exceeding this limitation! Miltenyi Biotec does not guarantee proper
function of the instrument and disposables above this value. However, when saving a program exceeding
3,200 rpr a pop-up screen will appear displaying a warning message and the current rpr value. Press OK to savethe program. Press Abort to re-edit the program.

Editing programs
To edit a program:
1. Select the Templates or User folder containing the required program.
INSTRUMENT OPERATION

4.5.2

Note: Files located in the Miltenyi and Favorites folders cannot be edited. The Miltenyi folder contains
gentleMACS Programs that have been optimized by Miltenyi Biotec for a variety of applications. These programs
cannot be altered. The Favorites folder contains shortcuts to the original programs located in the Templates,
Miltenyi, or User folders.
Note: The template programs A to E provided by Miltenyi Biotec can be used to create a user-defined program.
The newly created program has to be renamed and saved in one of the five User folders.
2. Highlight the program of interest. In this example, program_03 in the folder User_1 is selected.

4

3. Press Edit to open the respective program.
4. Highlight the required step. Edit the highlighted command by changing or adding required values (rpm, min, sec),
or use the following command buttons to modify the program:lDelete to remove the highlighted step from the list,l

Add to insert an additional step above the highlighted step.

Note: Steps can be highlighted using the touchscreen or the scroll buttons.
5. Select Save to store the modified file or Exit to discard all changes. When selecting Save, a pop-up window
displays the following options for selection:l
Save to overwrite the existing program,l

Save as to save the program with another name,l

Cancel to abort the process.

71

INSTRUMENT OPERATION

4.5.3

Creating program shortcuts into Favorites
Shortcuts to frequently used programs in the Miltenyi, Templates, or User folders can be created in theFavorites folder. These shortcuts can be used to quickly access the required programs.
1. Highlight the folder containing the required program.
2. Check the corresponding checkbox to select the program. Note that it is possible to select multiple programs. Any
program, highlighted or not, can be selected. In this example, three programs in the folder User_1 are selected fortransfer.

4

3. Press Save.
4. Select the Favorites folder in the pop-up window. Confirm by pressing OK.

4.5.4

Transfering programs to other folders
Programs can be transferred across folders and USB drives. Refer to table 4.6 for an explanation of programtransfer rights.

72

Note: The Favorites folder contains only links to programs in other folders and cannot be used to transfer
programs.
Note: Before transferring programs to a USB stick, ensure that the USB stick is inserted securely into the USB
port.
1. Highlight the folder containing the required program.

INSTRUMENT OPERATION

2. Check the corresponding checkbox to select the program. Note that it is possible to select multiple programs. In
this example, three programs in the folder User_1 are selected for transfer.

4
3. Press Save.
4. Choose the destination folder by pressing the folder icon in the pop-up window until the respective folder
appears. Press OK to save the programs, or Cancel to abort the process. In this example, the programs aretransferred to a USB stick.

5. Open the destination folder to ensure that the transfer was successful.

73

INSTRUMENT OPERATION

4

FolderDescription

Program transfer
rightsFavorites

It is recommended to create shortcuts to frequently used
programs in this folder.
The shortcut, when used, will run the latest version of theassociated program.
A maximum of 20 shortcuts can be created.

Shortcuts can only bedeletedMiltenyi

This folder contains gentleMACS Programs that have beendeveloped by Miltenyi Biotec for a variety of applications.

From Miltenyi to:- Favorites folder

Templates

Contains generic programs for tissue dissociation andhomogenization protocols. These programs provide an
excellent basis to develop and optimize customized programs
for user-specific applications. Altered templates have to berenamed and saved in a User folder.

From Templates to:- Favorites folder

User folder

There are a total of five user folders. Their names can be
modified using the Setup menu.

From any user folder to:- Favorites folder- USB stick- User folders

USB stickgentleMACS Programs provided by Miltenyi Biotec and user
programs can be transferred from the USB stick to the
instrument.

From USB stick to:- User folders

Table 4.6: Overview of the gentleMACS Octo Dissociator with Heaters folders and program transfer rights.

4.5.5

Deleting programs
To delete a program:
1. Select the User folder containing the required program.
2. Highlight the program to be deleted.
3. Press Delete to remove the program.
4. Press OK to confirm or Cancel to abort the process.

Note: Files located in the Miltenyi and Templates folder cannot be deleted.
Note: Deleting a shortcut in the Favorites folder will not delete the original program.

4.6

Setup
The Setup menu is used to manage the following device settings:

74l

Set LED – switch the LEDs on or offl

Set time – set the timel

Set date – set the date in the format dd-mm-yyyyl

Set folder name – name/rename User folderslSoftware version – get information on the software version that is installed on the instrument.

lSerial number – get information on the serial number of the instrument.

4.6.1

Set LEDTo set the LED for the instrument:
1. Press Set LED.
2. Press Set LED on or Set LED off as required.

4.6.2

Set Clock and date

INSTRUMENT OPERATION

The procedures to modify the time and date are identical. The following example shows the change of date.
1. Press Set date.
2. Press the desired parameter: day (dd), month (mm), and year (yyyy).
3. Modify the parameter using the touchscreen.
4. Press OK to confirm or Exit to cancel.

4.6.3

Set folder name
To name or rename User folders:4

1. Press Set folder name.
2. Select the required folder.
Note: Default values for User folders are User_1, User_2, User_3, User_4, User_5.
3. Enter the new name using the touchscreen. The folder name cannot exceed nine characters.
4. Press OK to save or Exit to cancel.

4.6.4

Software version
To display the software version:
Press Software version. The software versions and gentleMACS Program database version will be displayed.

4.6.5

Serial number
To display the serial number of the instrument:
Press Serial number. The serial number will be displayed.

75

4

76

INSTRUMENT OPERATION

5
Maintenance

5.1

Servicing
If the gentleMACS Octo Dissociator with Heaters needs servicing, send the instrument back to an authorized
MAINTENANCE

Miltenyi Biotec service provider. Ensure that all parts are decontaminated and sterilized (for details, refer tosection 5.2). Use the original packaging material.
Do not service the instrument yourself. Service needed on components inside the instrument must be carriedout only by personnel trained and authorized by Miltenyi Biotec. Servicing and an improperly serviced
instrument may result in mechanical hazards, optical radiation hazards, electric shock, or personally injury oreven death. Read the chapter Important safety information.

5.2

5Cleaning
Read the section Risk of electric shock in the chapter Important safety information. Unplug the power
cord before cleaning the instrument. Do not allow fluids or objects to enter the interior of the instrument.
Large amounts of accidentally spilled liquid are automatically collected in the Trough of the gentleMACS Octo
Dissociator with Heaters. Use a pipette to aspirate the liquid before removing the Trough for cleaning andsterilizing. If biohazardous material is or has been used always use personal safety equipment while removing
parts of the gentleMACS Octo Dissociator with Heaters for cleaning or sterilization.
In case of spillage or contamination, clean the instrument appropriately.
For decontamination, wipe the instrument with 70% ethanol. Clean the instrument only with cleaning agentsthat are non-corrosive and not damaging to the plastic housing of the gentleMACS Octo Dissociator with
Heaters. Suitable cleaning agents include household detergents and 70% ethanol. Do not use acetone or any
aggressive agents. If in doubt, contact Miltenyi Biotec Technical Support.
Note: Do not leave gentleMACS Heaters and Tubes attached to the instrument while it is not in use.
1. Unplug the power cord before cleaning the instrument.
2. Remove the Safety Shield.
3. Remove the Sleeves from the instrument for cleaning and/or sterilization.
4. Remove the Trough for cleaning.

77

MAINTENANCE

Note: To remove the Trough pull on one side of the Trough. Avoid pulling on both sides at the same time.

Figure 5.1: Disassembly of the gentleMACS Octo with Heaters for cleaning.

5

WARNING! Optical radiation emitted from gentleMACS Tube and Safety Shield illumination light emitting
diodes (LEDs) may be harmful to the eyes at close viewing distances when the Trough is removed. Do not stareat operating LEDs from a distance less than 20 cm when the Trough is removed. Read the chapter Importantsafety information.
5. The surface of the gentleMACS Octo Dissociator with Heaters should be cleaned with a cloth soaked with adetergent-containing aqueous solution or 70% ethanol. Carefully wipe all parts.

Note: For cleaning, wipe the gentleMACS Heaters carefully with a soft cloth soaked with 70% ethanol. Do not
use any detergents. Do not soak the gentleMACS Heaters directly into any cleaning solution.
6. Repeat the procedure with another cloth soaked with distilled water.
7. If the homogenized substances are not water-soluble, it is recommended to wipe all parts with ethanol. Contact
Miltenyi Biotec Technical Support for further advice.
8. If necessary, exchange the Sleeves and the Trough.
9. Reassemble the parts while ensuring that the removes parts snap into position correctly. Ensure that theorientation of the Trough and the Safety Shield is correct.

5.3

Instrument disposal
The gentleMACS Octo Dissociator with Heaters must be disposed of in accordance with the biosafety level of its
use. Potentially contaminated parts must be sterilized before disposal, in accordance with local laboratory
regulations.
WARNING! Decontamination is the responsibility of the operator of the instrument. Contaminated instruments
or contaminated parts of the instrument must not be sent or given to any person.

78

6

Error

Cause of error

RemedyLiquid droplets appear in
the TroughTube is not closed properly.

Close the tube tightly beyond
first resistance.

Sample is jammed between the cap and thetube and damages the seal. This might cause
liquid to run trough the seal.

The top area of the cap and thetube must be perfectly clean forproper functioning of the seal.

TROUBLESHOOTING

Troubleshooting

Replace the tube.
Tube seal is leaky.
Exchange Sleeve.
Sleeve is worn.
Tube is blocked during arun.

Inappropriate sample material.

Refer to the respective data
sheet for technicalspecifications of the tubes.

Program is automaticallyaborted and the instrument
displays the message"Motor is blocked. Program
was aborted."

If the motor is blocked due to a high resistanceof the sample material, the instrument will try
three times to get started again.

Process a smaller amount oftissue, or use a different
gentleMACS Program.

If the blockage can be resolved, the instrument
will continue the run of the program. If theblockage cannot be resolved, the program willautomatically be aborted.

Check, if tissue is trapped
between the stator and rotor.
Release tissue, reinstall tube,
and press continue to resume
the program.

Tube comes off the
instrument

Tube is not correctly fitted into the snap-fit of
the Trough.

Ensure correct installation of the
tube.

Tube is not closed properly.

Close the tube tightly beyond
first resistance.

A warning message appears
in a pop-up window whensaving an edited program.
"Input error - Maximum
rounds per run (3200)
exceeded!"

The duration of the program exceed 3,200
rounds per run (rpr). It is not recommended torun programs exceeding this limitation!
Miltenyi Biotec does not guarantee proper
function of the instruments and disposables
above this value.

Press OK to save the program.
Press Abort to re-edit the
program.

New program containing
iterations cannot be saved.
The instrument displays anerror message with thecause of the error.

"Loop command is faulty. End loop commandis missing." indicates that the end loop
command has not been specified.

Close iterations by adding an
end loop command.

"Loop command is faulty. The command isnested." indicates that there are more than
one loop commands.

Use only one loop commandand close the iteration byadding an end loop command.

"Loop command is faulty. Loop has no body."
indicates that there is no command between
the loop and end loop commands.

At least one program step must
be included between a loop
and end loop command. Add
steps and define commands
that shall be iterated.

The heater was removed.

Reinstall heater.

The heater is not installed properly.

Make sure that the heater isinstalled properly.
Carefully clean the heater

A warning message "Theheater was removed!"
appears in a pop-up
window, while running aheaters program.

6

The electrical contacts of the instrument or the79Error

Cause of error

Remedyheater are dirty or corroded.

contacts of the instruments andthe resp. heater using a cloak
soaked with water or 70%
ethanol.
Carefully check the heater forsigns of damage. If no visible
signs of damage can bedetected, switch the instrument
OFF and ON again and retry to
run the program.

TROUBLESHOOTING

Contact Technical Service.
Program is assigned to a
tube position and displays ared error message.

Program contains a heating step and no heater
is installed.

Press the tube position on the
instrument's display for more
information.
Install heater.

Shortcut to a program
cannot be created in theFavorites folder. The
instrument displays themessage "Memory is wornout. File cannot be saved."

The Favorites folder has reached the maximum
limit of 20 shortcuts.

Delete other program shortcuts
in the Favorites folder.

Folder cannot be accessed.
The instrument displays themessage "Program folder
cannot be opened!".
Table 6.1: Errors, sources of errors, and user actions

6

80

Restart the device and try again.
If the problem persists, contact
Miltenyi Biotec TechnicalSupport.

7

Technical data

Specification

ColorsYellow / orange

Size

465x285x185 mm (Length x Width x Height)

Weight

11 kg
TECHNICAL SPECIFICATIONSTechnical specifications

Main power
Power consumption

max. 450 W

Supply voltage

100-240 VAC, 50/60 Hz

Fuse

2xT3.15AH250V

Motor
Rotational Speed

20-4,000 rpm

Rotational direction

Clockwise or counter-clockwise

Rotational acceleration

100r/s²

Torquemax. 150 mNm;
Overload restriction over the range of 200-4,000 rpm

7

Conditions of operation
Working temperature without use of heaters+4 °C to +35 °C

Working temperature with use of heaters+4 °C to +30 °C

Humidity

15-80%, relative, non-condensing

Altitude

Maximum 2,000 m

Storage temperature-15 °C to +70 °C

USB interface(for service purposes and for loading andstorage of programs)

Pin 1: USB + 5 V
Pin 2: USB Pin 3: USB +Pin 4: USB GND

RS232 port

Pin 1, 4, 6, 9: NC
Pin 2: RxD
Pin 3: TxD
Pin 5: GND
Pin 7: CLK
Pin 8: CTS

CAN port

Pin 1, 4, 5, 8: NC
Pin 2: CAN-L
Pin 3, 6: GND
Pin 7: CAN-H
Pin 9: 24VDC/1A

Maximum number of programs

500

Number of heatable positions

881

Technical data

Specification

Maximum noise emissions
Sound pressure level at workstation

86 dB(A) +/- 2.5 dB(A)Sound power level
Measurement operation conditions:
8x C Tubes containing 500 µl of liquid,
processed at 4,000 rpm

92 dB

Working temperature heaters

37 °C

Table 7.1: Technical specifications of the gentleMACS Octo Dissociator with Heaters.

The gentleMACS Octo Dissociator with Heaters is a Protection Class I instrument and must be plugged into aground power outlet. The main power supply cord and plug of the instrument must comply with following
specifications (USA and Canada only): UL listed and KAM cord, minimum type SJ, minimum 18 AWG,
3 conductors. Rated for a minimum temperature of 60 °C. Provided with grounding-type (NEMA 5-15P-9
TECHNICAL SPECIFICATIONS

attachment plug, rated 125 VAC, 10 A. Opposite end terminates in IEC 320 style connector, rated 125VAC, 10A.
Conditions of operations: Supply voltage fluctuations up to +/- 10% of the nominal voltage. Transient overvoltages present on the main supply: category II.
The instrument is suitable for rated pollution degree 2.
This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant toPart 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmfulinterference in a residential installation. This equipment generates, uses and can radiate radio frequency
energy and, if not installed and used in accordance with the instructions, may cause harmful interference toradio communications. However, there is no guarantee that interference will not occur in a particular

7

installation.
If this equipment does cause harmful interference to radio or television reception, which can be determined byturning the equipment off and on, the user is encouraged to try to correct the interference by one or more of
the following measures:lReorient or relocate the receiving antenna.

l

Increase the distance between the equipment and the receiver.

lConnect the equipment to an outlet on a circuit different from that to which the receiver is connected.

lConsult the dealer or an experienced radio/TV technician for help.

Changes or modifications of the instrument, unless expressly approved by Miltenyi Biotec, may void authority
to operate the instrument pursuant to FCC 47 CFR.
For other safety considerations, refer to the product label, or visit www.miltenyibiotec.com.
Design and specifications are subject to change without notice.

82

EC / EU Declaration of conformity
This declaration of conformity is issued under the sole responsibility of the manufacturer:
Miltenyi Biotec B.V. & Co. KG
Friedrich-Ebert-Straße 68
51429 Bergisch Gladbach
Germany
This declaration relates exclusively to the product in the state in which it was placed on the market andexcludes components which are added and/or operations carried out subsequently by the final user.
The declaration of conformity refers to the machinery identified as follows:
Description: Laboratory equipment
TECHNICAL SPECIFICATIONS

Model: gentleMACS Octo Dissociator with Heaters
The machinery complies with all essential requirements of the following directives:
2006/42/EC Machinery2014/30/EU Electromagnetic compatibility
2011/65/EU Restriction of the use of certain hazardous substances in electrical & electronic equipment
The machinery is in conformity with the following harmonized standards:
EN 61010-1:2010

7

EN 61010-2-010:2014
EN 61010-2-051:2015
EN 61326-1:2013
Person authorized to compile the relevant technical documentation:
Dr. Bernd Schröder
Global Head Regulatory Affairs
Miltenyi Biotec B.V. & Co. KG
Friedrich-Ebert-Straße 68
51429 Bergisch Gladbach
Germany83

UK Declaration of Conformity
This declaration of conformity is issued under the sole responsibility of the manufacturer:
Miltenyi Biotec B.V. & Co. KG
Friedrich-Ebert-Straße 68
51429 Bergisch Gladbach
Germany
This declaration relates exclusively to the product in the state in which it was placed on the market andexcludes components which are added and/or operations carried out subsequently by the final user.
The declaration of conformity refers to the machinery identified as follows:
Description: Laboratory equipment
TECHNICAL SPECIFICATIONS

Model: gentleMACS Octo Dissociator with Heaters
The machinery complies with all essential requirements of the following legislations:
Supply of machinery (Safety) regulations 2008
The Restriction of the Use of Certain Hazardous Substances in Electrical and Electronic Equipment Regulations
2012
Electromagnetic Compatibility Regulations 2016
The machinery is in conformity with the following UK designated standards:

7

EN 61010-1:2010
EN 61010-2-010:2014
EN 61010-2-051:2015
EN 61326-1:2013
Person authorized to compile the relevant technical documentation:
Dr. Bernd Schröder
Global Head Regulatory Affairs
Miltenyi Biotec B.V. & Co. KG
Friedrich-Ebert-Straße 68
51429 Bergisch Gladbach
Germany84

8Technical support

For technical support, contact your local Miltenyi Biotec representative or Miltenyi Biotec Technical Support at
Miltenyi Biotec headquarters:
Miltenyi Biotec B.V. & Co. KG
Friedrich-Ebert-Straße 68
51429 Bergisch Gladbach
Germany
Phone: +49 2204 8306 3803
TECHNICAL SUPPORTEmail: technicalsupport@miltenyi.com
Visit www.miltenyibiotec.com for local Miltenyi Biotec Technical Support contact information.

8

85

8

86

TECHNICAL SUPPORT9Legal notes

Limited warranty
Except as stated in a specific warranty statement which may accompany this product or as otherwise agreed inwriting by an authorized representative of Miltenyi Biotec, Miltenyi Biotec’s warranty to you, the original
purchaser and end user (“you” or “your”), with respect to the product accompanied by this limited warranty
shall be subject to the following provisions and the general terms and conditions of sale of the company within
the Miltenyi Biotec group which supplied the product in effect at the date of purchase. Those terms andconditions of sale may vary by country and region. Nothing in this document should be construed as
constituting an additional warranty.
Miltenyi Biotec warrants that this product will operate or perform substantially in conformance with Miltenyi
Biotec's published specifications and be free from material defects in material and workmanship, whensubjected to normal, proper, and intended usage by properly trained personnel, for the period of time set forthin the product documentation or package inserts accompanying the product (the “Warranty Period”).
LEGAL NOTES

9.1

Miltenyi Biotec agrees, during the Warranty Period, to repair or replace, at Miltenyi Biotec's option, thedefective product so as to cause the same to operate in substantial conformance with said publishedspecifications; provided that you shall (a) promptly notify Miltenyi Biotec in writing upon the discovery of any
nonconformity or defect, which notice shall include the product model and serial number (if applicable) anddetails of the warranty claim; and (b) return the nonconforming or defective product to Miltenyi Biotec, freight9

prepaid, only after receipt of a Return Material Authorization (“RMA”) from Miltenyi Biotec, which may include
biohazard decontamination procedures and other product-specific handling instructions, if applicable.
Miltenyi Biotec shall have no obligation to make repairs, replacements, or corrections to the product or any
component thereof required, in whole or in part, as the result of (i) normal wear and tear, (ii) improper
handling, installation, operation, storage, service, maintenance, or repair, (iii) failure to follow the instructions,cautions, warnings, and notes set forth in the product documentation provided with the product or provided
by Miltenyi Biotec from time to time, (iv) abnormal use, misuse, neglect, abuse, mishandling, misapplication,
modification, or alteration of the product, (v) use of the product in a manner for which it was not designed, (vi)causes external to the product such as, but not limited to, power failure or electrical power surges, (vii) use of
the product in combination with equipment, accessories, consumables, or software not supplied or approvedby Miltenyi Biotec, or (viii) accident, disaster, or acts of God. ANY INSTALLATION, MAINTENANCE, REPAIR,SERVICE, OR ALTERATION TO OR OF, OR OTHER TAMPERING WITH, THE PRODUCT PERFORMED BY ANY PERSONOR ENTITY OTHER THAN MILTENYI BIOTEC AUTHORIZED PERSONNEL WITHOUT MILTENYI BIOTEC’S PRIORWRITTEN APPROVAL, OR ANY USE OF REPLACEMENT PARTS NOT SUPPLIED BY MILTENYI BIOTEC, SHALLIMMEDIATELY VOID AND CANCEL ALL WARRANTIES WITH RESPECT TO THE AFFECTED PRODUCT.

87

Miltenyi Biotec’s warranty does not cover products sold AS IS or WITH ALL FAULTS, or which had its serial
number defaced, altered, or removed, or any consumables or parts identified as being supplied by a third party.
Miltenyi Biotec must be informed promptly if a claim is made under this limited warranty. If a material or
manufacturing defect occurs within the Warranty Period, Miltenyi Biotec will take the appropriate steps, at
Miltenyi Biotec's option, to make repairs, replacements, or corrections to the product or any component
thereof as may be required to restore the full usability of your product. If Miltenyi Biotec determines that a
product for which you have requested warranty services is not covered by the warranty hereunder, you shall
pay or reimburse Miltenyi Biotec for all costs of investigating and responding to such request at Miltenyi
Biotec’s then prevailing time and materials rates. If Miltenyi Biotec provides repair services or replacement parts
that are not covered by this warranty, you shall pay Miltenyi Biotec therefor at Miltenyi Biotec’s then prevailingtime and materials rates.
THE OBLIGATIONS CREATED BY THIS WARRANTY STATEMENT TO REPAIR OR REPLACE A DEFECTIVE PRODUCTARE EXCLUSIVE AND SHALL BE THE SOLE REMEDY OF BUYER IN THE EVENT OF A DEFECTIVE PRODUCT. EXCEPTAS EXPRESSLY PROVIDED IN THIS WARRANTY STATEMENT, MILTENYI BIOTEC HEREBY DISCLAIMS ALL OTHERWARRANTIES, WHETHER EXPRESS OR IMPLIED, ORAL OR WRITTEN, WITH RESPECT TO THE PRODUCT,INCLUDING WITHOUT LIMITATION ALL IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR ANY
PARTICULAR PURPOSE. MILTENYI BIOTEC DOES NOT WARRANT THAT THE PRODUCT IS ERROR-FREE OR WILLACCOMPLISH ANY PARTICULAR RESULT.
UNDER NO CIRCUMSTANCES WILL MILTENYI BIOTEC BE LIABLE FOR ANY LOSS OF USE, INTERRUPTION OF
BUSINESS, OR ANY INDIRECT, SPECIAL, INCIDENTAL, PUNITIVE, OR CONSEQUENTIAL DAMAGES OF ANY KIND(INCLUDING LOST PROFITS) REGARDLESS OF THE FORM OF ACTION WHETHER IN CONTRACT, TORT (INCLUDINGLEGAL NOTESNEGLIGENCE), STRICT PRODUCT LIABILITY, OR OTHERWISE, EVEN IF MILTENYI BIOTEC HAS BEEN ADVISED OF
THE POSSIBILITY OF SUCH DAMAGES. IN NO EVENT SHALL THE TOTAL LIABILITY OF MILTENYI BIOTECHEREUNDER EXCEED THE GREATER OF 100.00 EUROS OR THE AMOUNT YOU ACTUALLY PAID FOR THE
PRODUCT GIVING RISE TO SUCH LIABILITY, REGARDLESS OF THE CAUSE OF ACTION, IN CONTRACT, TORT,STRICT LIABILITY, OR OTHERWISE.

9NOT ALL JURISDICTIONS ALLOW SUCH LIMITATIONS OF DAMAGES SO THE FOREGOING LIMITATIONS MAY NOTAPPLY TO YOU. This warranty statement gives you specific legal rights and you may have other rights, which
may vary from jurisdiction to jurisdiction.

9.2

Trademarks
gentleMACS, MACS, and the Miltenyi Biotec logo are either registered trademarks or trademarks ofMiltenyi Biotec and/or its affiliates in various countries worldwide.

88USA/Canada
Miltenyi Biotec Inc.
2303 Lindbergh Street
Auburn, CA 95602, USA
Phone 800 FOR MACS
Phone +1 866 811 4466
Fax +1 877 591 1060
macsus@miltenyi.com
Australia
Miltenyi Biotec
Australia Pty. Ltd.
Unit 11, 2 Eden Park Drive
Macquarie Park NSW 2113
Australia
Phone +61 2 8877 7400
Fax +61 2 9889 5044
macsau@miltenyi.com

Benelux
Miltenyi Biotec B.V.
Sandifortdreef 172333 ZZ Leiden
The Netherlands
macsnl@miltenyi.com
Customer service
The Netherlands
Phone 0800 4020120
Fax 0800 4020100
Customer service Belgium
Phone 0800 94016
Fax 0800 99626
Customer service Luxembourg
Phone 800 24971
Fax 800 24984
China
Miltenyi Biotec Technology &Trading (Shanghai) Co., Ltd.
Room A401, 4/F
No. 1077, Zhangheng Road
Pudong New Area
201203 Shanghai, P.R. China
Phone +86 21 6235 1005-0
Fax +86 21 6235 0953
macscn@miltenyi.com.cn
FranceMiltenyi Biotec SAS
10 rue Mercoeur
75011 Paris, France
Phone +33 1 56 98 16 16
macsfr@miltenyi.com

Hong Kong
Miltenyi Biotec Hong Kong Ltd.
Unit 301, Lakeside 1
No. 8 Science Park West Avenue
Hong Kong Science Park
Pak Shek Kok, New Territories
Hong Kong
Phone +852 3751 6698
Fax +852 3619 5772
macshk@miltenyi.com.hk
Italy
Miltenyi Biotec S.r.l.
Via Paolo Nanni Costa, 30
40133 Bologna
Italy
Phone +39 051 6 460 411
Fax +39 051 6 460 499
macsit@miltenyi.com
Japan
Miltenyi Biotec K.K.
NEX-Eitai Building 5F
16-10 Fuyuki, Koto-ku,
Tokyo 135-0041, Japan
Phone +81 3 5646 8910
Fax +81 3 5646 8911
macsjp@miltenyi.com

Nordics and Baltics
Miltenyi Biotec Norden AB
Medicon Village
Scheeletorget 1
223 81 Lund
Swedenmacsse@miltenyi.com
Customer service Sweden
Phone 0200 111 800
Fax +46 280 72 99
Customer service Denmark
Phone 80 20 30 10
Fax +46 46 280 72 99
Customer service
Norway, Finland, Iceland
and Baltic countries
Phone +46 46 280 72 80
Fax +46 46 280 72 99
Singapore
Miltenyi Biotec Asia Pacific Pte Ltd
438B Alexandra Road, Block B
Alexandra Technopark#06-01
Singapore 119968
Phone +65 6238 8183
Fax +65 6238 0302
macssg@miltenyi.com
South Korea
Miltenyi Biotec Korea Co., Ltd.
Arigi Bldg. 8F
562 Nonhyeon-roGangnam-gu
Seoul 06136, South Korea
Phone +82 2 555 1988
Fax +82 2 555 8890
macskr@miltenyi.com

Spain
Miltenyi Biotec S.L.
C/Luis Buñuel 2
Ciudad de la Imagen
28223 Pozuelo de Alarcón(Madrid)
Spain
Phone +34 91 512 12 90
Fax +34 91 512 12 91
macses@miltenyi.com
Switzerland
Miltenyi Biotec Swiss AG
Gibelinstrasse 274500 Solothurn
Switzerland
Phone +41 32 623 08 47
Fax +49 2204 85197
macsch@miltenyi.com
United Kingdom
Miltenyi Biotec Ltd.
Almac House, Church Lane
Bisley, Surrey GU24 9DR, UK
Phone +44 1483 799 800
Fax +44 1483 799 811
macsuk@miltenyi.com
www.miltenyibiotec.com

Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. gentleMACS, MACS, and the Miltenyi Biotec logo
are registered trademarks or trademarks of Miltenyi Biotec and/or its affiliates in various countries worldwide.
Copyright © 2022 Miltenyi Biotec and/or its affiliates. All rights reserved.

140-004-386.06

Germany/Austria
Miltenyi Biotec B.V. & Co. KG
Friedrich-Ebert-Straße 68
51429 Bergisch Gladbach
Germany
Phone +49 2204 8306-0
Fax +49 2204 85197
macsde@miltenyi.com