Every cell is unique
The C1™ Single-Cell Auto Prep System—
Defy the Law of AveragesnnSingle-cell precision—greater accuracy to measure differences in gene expression profilesnnEasy to use—simplified cell isolation and preparation with a streamlined workflow and intuitive
interfacenn

Fast—cell input-to-data in less than a daynn

All in one—comprehensive, automated workflow generates reproducible and reliable results

nnby pipette, and processing cells are laborious, timeconsuming, and prone to introducing errors. In addition,
averaging cell pools masks many important functions
within a single cell. Geneticists and clinical researchers
have been looking for a complete workflow that can examine and differentiate individual cells and group themaccording to unique genomes and transcriptomes while
minimizing technical noise.
The C1 Single-Cell Auto Prep System takes an entirely
new approach, based on Fluidigm innovative microfluidic technology that enables you to rapidly and reliably
isolate, process, and profile individual cells for genomic
analysis. For the first time, you can extract, reverse transcribe, preamplify, and ultimately detect and analyze cell
activity using just one technology, reducing the variability
caused by multi-platform technical errors.

Flexible—expandable into whole transcriptome
and variant discovery

Individual cells vary as much as the researchers whostudy them. Traditional methods of extraction, isolation

The C1 Single-Cell Auto Prep System enables you to study
cell differentiation, measuring individual cell responses tospecific stimuli, verify critical disease biomarkers, validate
RNAi knockdown, and conduct candidate drug screens.

Components of the C1 singlecell Auto Prep System
The C1 Single-Cell Auto Prep System isthe world’s first commercially available,
automated single-cell isolation and preparation system for genomic analysis. The
C1 System provides an easy and highly reproducible gene expression workflow toprocess 96 single cells across 96 mRNAtargets. In this format, our single-cellworkflow enables you to measure theexpression of hundreds of genes in justa few hours compared to experimentsthat would normally take days using traditional systems. With this new system
you can rapidly explore unique attributesof individual cells without the technicalvariability of a standard gene expression
workflow.
The C1 Single-Cell Auto Prep System consists of the following:
C1 Single-Cell Auto Prep System—breakthrough, bench-top automation of isolation,lysis, and preamplification of single cells
nn C1 Single-Cell Auto Prep Array IFC—
proprietary integrated fluidics circuit that facilitates capture and highly paralleled preparation of 96 individual cells
nn C1 Single-Cell Auto Prep Reagent Kit—a ready-to-use reagent kit to support cellsuspension, lysis, and purification
nnUp to 96 Single Cells in Just One HourThe new C1 Single-Cell Auto Prep System provides a simple “plugand play” workflow to reproducibly prepare 96 individual cells inapproximately 1 hour of hands-on time. The simple user interface
and comprehensive C1 System workflow helps you to:
Capture cells—load batches of cells in a single pipetting step andrapidly separate into 96 individual chambers for preparation
nn Verify—use an “in-process” quality control checkpoint to verify thenumber of captured cells and distinguish live and dead cells to preserve data integrity
nn Lyse—use a rapid direct cell lysis method to save time and cost without RNA purification
nn Reverse transcribe and preamplify—cDNA synthesis and specifictarget amplification occur on the same sample without reagent mixing and sample transfer
nn Harvest—all preamplified products are pooled, harvested, and transferred to the BioMark™ HD System for quantitative PCR analysis
nnThe C1 Single-Cell Auto Prep System coupled with the BioMark
HD System streamlines gene expression analysis through seamless effort and significant time savings with support for up to96 individual cells across 96 transcripts. With automation andminimal costs the C1 Single-Cell Auto Prep System dramaticallyincreases productivity to further accelerate expression profilingstudies. With an optimized protocol, pre-formulated reagent kit,
and disposable arrays, you can achieve single-cell accuracy with“load and go” productivity.
CaptureWash

VerifyLyse

C1 Single-Cell Auto Prep SystemRTPreAmp

qPCR

Analyze

BioMark HD System

Your cells and your research are anything but average. Learn how
the C1 Single-Cell Auto Prep System can put your gene expression
work above the curve at fluidigm.com/c1system

fluidigm.com/c1system© 2012 Fluidigm Corporation. All rights reserved. Fluidigm, the Fluidigm logo, BioMark, C1, DELTAgene, and Dynamic Array are trademarks or registered trademarks ofFluidigm Corporation in the U.S. and/or other countries. All other trademarks are the property of their respective owners.
For Research Use Only. Not for use in diagnostic procedures.
BR100-5477 B2 9/2012

Corporate Headquarters
7000 Shoreline Court, Suite 100
South San Francisco, CA 94080 USA
Toll-free: 1.866.FLUIDLINE | Fax: 650.871.7152
www.fluidigm.com

Sales
North America | +1 650 266 6170 | info-us@fluidigm.com
Europe/EMEA | +33 1 60 92 42 40 | info-europe@fluidigm.com
Japan | +81 3 3662 2150 | info-japan@fluidigm.com
China (including Hong Kong) | +86 21 3255 8368 | info-china@fluidigm.com
Asia | +1 650.266.6170 | info-asia@fluidigm.com
Latin America | +1 650 266 6170 | info-latinamerica@fluidigm.com