Cytation™3 combines automated digital microscopy andconventional microplate reading in one instrument. Its uniquepatent pending design is ideal for research and assay developmentapplications in the field of cell biology. With an emphasis on livecell assays, Cytation3 features temperature control to 45 °C, orbitalshaking, CO2/O2 gas control and support for time lapse studies.

In contrast with complex image analysis software interfaces availabletoday, BioTek’s Gen5 is specifically designed for those familiarwith microplate reader software and requires minimal training.
Equipped with patented Hybrid Technology™ for microplatereading, Cytation3 offers high performance filter-based optics andhigh-flexibility monochromator optics for unmatched applicationversatility.

Features:•   Automated digital microscopy and/or multi-mode microplatedetection in one instrument•High quality images: Olympus objectives, 16-bit gray scale CCDcamera, Semrock filters•Modular and upgradable: Microscopy only, microplate readingonly, or both

•Temperature control up to 45 °C•Flexible sample format: Accommodates 6- to 384-well plates,microscope slides and T25 cell culture flasks•CO2/O2 gas control and monitor accessory•

Dual reagent injector accessory for inject/read assays•End point, time-lapse and montage assays•Automatic cell counting, sub-population analysis and imagestatistics•Patented Hybrid Technology™ microplate reading mode with highperformance filter-based optics and high-flexibility monochromatoroptics•Affordable automation: Automated XY stage, auto focus, autoexposure, automated image capture, auto LED intensity•Inverted fluorescence (4 LED and filter cube assembly capacity)and bright field microscopy•   From 2x for full-well imaging to 20x for sub-micron resolutionof intra-cellular details

Models:

Specifications:CYT3V:CYT3FV:CYT3MV:CYT3MFV:CYT3MF:CYT3M:CYT3F:

GeneralCytation3 w/imagingCytation3 w/filter optics and imagingCytation3 w/mono optics and imagingCytation3 w/mono and filter optics and imagingCytation3 w/mono and filter opticsCytation3 w/mono opticsCytation3 w/filter optics

Optional Accessories:• CO2/O2 Gas Controller Module• Dual Reagent Injector Module• BioStack™ Microplate Stacker• Take3™ Micro-Volume Plate• Gen5™ Secure for 21 CFR part 11 compliance• Luminescence, Fluorescence and Absorbance Test Plates

Typical Applications:• Cell imaging and analysis• Cell proliferation• Cytotoxicity• Protein expression• Biomarker quantification• Drug discovery• Genetic analysis• Drug absorption and metabolism• Biologics drug discovery and development• Environmental testing• Food safety• Nucleic acid quantification• Protein quantification

Imaging modes:			 Fluorescence and brightfieldDetection method:			 Monochromators: FL, Lum., UV-Vis Abs.				 Filters: FL, TRF, FP, Lum.
Read method:			 End point, time-lapse, kinetic, well mode, montageLabware type:			 6- to 384-well plates, microscope slides, cell culture flasks (T25)				 Compatible with Take3™ Micro-Volume Plates with 2 µL					microspotsTemperature control:			 To 45 °C; ±0.5 °C at 37 °CShaking:			 Linear, orbital, double orbitalAutomation:			 Compatible with BioStack™ and 3rd party automationCO2 and O2 control:			 0 – 20% CO2 control and 1 – 19% O2 control, with optional					Gas ControllerSoftware: 			 Gen5™ Data Analysis Software

ImagingLight source:			 High power, user-replaceable LEDsCamera: 			 16-bit gray scaleFilter cube capacity:			 4 onboard, user-replaceable filter cubesObjective capacity:			 2 onboard, user-replaceable objectivesAvailable objectives:			 2x, 4x, 10x, 20xImage collection rate:			 96 wells: 1 image/well, 20x, 8 minutes

FluorescenceIntensity		Sensitivity:			Monochromators:				 Top: Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate)				 Bottom: Fluorescein 5 pM typical (0.5 fmol/well 384-well plate)				Filters/mirrors: Fluorescein 1pM typical (0.1 fmol/well 384-well					plate)Light source:			 Xenon flash lampWavelength selection:			 Double grating monochromators (top and bottom)				 Deep blocking bandpass filters/dichroic mirrors (top)Wavelength range:			 Monochromators: 250 – 700 nm				 Filters: 200 – 700 nm (850 nm option)Dynamic range: 			 5 decadesDetection system:			 Two PMT detectors: one for monochromator system, one for					filter system

Fluorescence Polarization			Sensitivity:		Wavelength range:		

5 mP at 1nM fluorescein typical320 – 700 nm (850 nm option)

Time-Resolved Fluorescence

BPAE cells, 20x, from FluoCells®
prepared slide #2.

HepG2 cells, Caspase3, 20xmagnification.

Sensitivity:		 Europium 100 fM typical with filters (10 amol/well in 384-well				plate)			 Europium 1200 fM typical with monos (120 amol/well in 384				well plate)Light source:		 Xenon flash lampWavelength range:		 Monos: 250 – 700 nm			 Filters: 200 – 700 nm (850 nm option)

Luminescence			

Sensitivity:					Wavelength range:		Dynamic range: 		

Monochromators: <20 amol ATP typical (flash)Filters: <10 amol ATP typical (flash)300 – 700 nm>6 decades

Absorbance3T3 cells, 4x magnification.
FluoCells® is a registered trademark of Molecular Probes, Inc.
BioTek’s Hybrid Technolgy™ is protected under US Patent 8,218,141.

Light source:			 Xenon flash lampWavelength selection:			 MonochromatorWavelength range:			 230 – 999 nm, 1 nm incrementBandpass: 			 4 nm (230 – 285 nm), 8 nm (>285 nm)Dynamic range:			 0 – 4.0 ODResolution:			 0.0001 OD		Reagent DispensersNumber:		Dispense volume:		Dead volume:		Plate geometry:		Dispense precision:		Dispense accuracy:		

2 syringe pumps5 – 1000 µL in 1 µL increment1 mL, 100 µL with back flush6- to 384-well microplates<2% at 50 – 200 μL±1 μL or 2%*Specifications subject to change.                         Rev. 04/19/13