DNABARCODINGPioneering
Complete AuthenticityatOUTLINE• QUALITY
INTHE
BOTANICALWORLDDNA• INDENAQUALITY UNIQUENESSBARCODINGPioneering• DNA
TESTING OF PLANTS
Complete Authenticity• DNA TESTING OF EXTRACTSatQUALITY OF BOTANICALSAND:
KEY QUALITY PARAMETERSAUTHENTICITY AND TRACEABILITY
THROUGH PLANTS PRODUCTION PATHWAY• Consistent physiological Activity of the Plants and their derivatives
depends on the consistency of the composition of their constituents,
which are affected by the following main factors through their cultivationand handling:A.

Plant genus/species (possible plant variety/cultivar/chemotype/part)

B.

Geographical origin (where plants are collected, if wild or cultivated)

C.

Cultivation–Harvesting

D.

Processing (drying, grinding)E.

Storage/freezing

F.

Transportation

TR
A
CKI
N
GT
RA
CING

AUTHENTICITY
30%

THE

IS IN THE
OF EXTRACTS USED.

GENERAL LABEL?
NOT ENOUGH.
Technological improvement
and leaps forward in R&D lead tostronger demand for multiple certifications

BOTANICAL ID CHRONICLES.

INDENA QUALITY SYSTEM• INDENA guarantees the QUALITY and theCONSISTENCY of extracts composition throughout
a process which deeply touches all stages of theproduction, from cultivation to the finishedproduct.
• In addition, the Indena’s quality systemcomprises DNA ANALYSIS, being genomic
identification part of its own DNA, for which thecompany is pioneering identification research.

PLANT IDENTIFICATION• The first step in QUALITY CONTROL of botanical preparations isensuring the CORRECT IDENTIFICATION OF THE PLANTS used.
Plant
taxonomy

Macroscopic

Microscopic

Chemotaxonomy

Genomic

GENOMIC IDENTIFICATION
DNAbarcodingDNAfingerprinting

GENERAL

PCR-based
DNA-NGS

DETAILEDOFTHE FIRST CHALLENGE.

BOTANICAL DNA IDENTIFICATION• For plants there is no universal DNA barcoding methodology:• each plant needs a dedicated method,• developed on its own genome.
•DNA SEQUENCING-BASED TESTS:

1. are emerging as highly reliable and powerful tools to authenticate
botanicals, to identify the species and varieties of medicinal plants;
2. play an important role inside the dietary supplement industry, since
adulteration is a serious concern.

DNA TESTING
In some cases it is necessary to resort toDNA-BASED METHODOLOGIES toprecisely define and identify species orvariety levels, through a3-STAGE DNA TESTING:
1.

EXTRACTION

through a standard
protocol to extract
the DNA2.

AMPLIFICATION

usually made through
PCR (PolymeraseChain Reaction) tohave a consistentamount of specific
DNA to be analyzed

3.

MEASUREMENT
by analysis ofDNA sequences

INDENA’S APPROACH
TO GENOMIC IDENTIFICATION
DNA barcoding is orthogonal: one single technique may not be enough
Botanical background to structure the DNA methodologies

Ability to use the whole range of DNA available techniques
Availability of authenticated plant material to develop highly reliable DNA tests
Where, when and how to collect the reference species to be used as “certified”
controls to validate DNA methods
Ability to identify unknown species

INDENA CAN
The first challenge:QUALITY
TRACEABILITY
AUTHENTICITY

IT USES.

In consideration of the importance ofthewithin Indena quality system andin order to make the communicationof such analysis more immediate andeasily usable by clients,

OUR
CERTIFICATIONTRADEMARKBUT…
Indena’s clients buy and use.

OFTHE NEXT CHALLENGE.

TAKES UP THE CHALLENGE…
!
First application:- Bilberry Extract

ISEffectiveness

Authenticity:

ReliableEXTRACTION
PURIFICATIONAMPLIFICATIONIDENTIFICATIONTHE
The amplified region
must be univocal?

Indena’s clients have now the possibility to use
also for, in line with the one
for plants, and they can choose one or both of themaccording to their specific needs:

OUR
CERTIFICATIONTRADEMARKClients have now the opportunity
to experience themselves
absolute certainty about
the quality of plants and extracts.

EXCLUSIVEPARTNERSHIP• Through an exclusive agreement with Hyris Ltd, and drawing on thesolid Indena database of reference compiled over decades, DNAfingerprint analysis on plant samples can be carried out in the fieldeven by unskilled personnel.

• INDENA HOLDS PREFERENTIAL ACCESS TO THIS BREAKTHROUGHTECHNOLOGY FOR ON-SITE IDENTIFICATION OF BOTANICALSPECIES, EXCLUSIVELY FOR THE DIETARY SUPPLEMENT INGREDIENT
INDUSTRY.
• The device that makes this possible is the bCUBE®, made by HyrisbCUBE® byAs small as a coffee mug, the bCUBE
can analyze starting material straightfrom the field in roughly an hour.

Even when carried out thousands ofmiles away from the Indena labs, thetest results can be shared on aproprietary cloudbased platform inreal time.

The GPS data can be recorded toenable perfect tracing.

bCUBE® by

THIS OPPORTUNITYWITH THE WORLD AND STAY TUNEDFOR FURTHER ACTIVITIES’NEWS!