CAPCELL PAKADME-HR / INERT ADME-HRA Cage-Structured C12 column Achieving Strong Retentionfor Polar Analytes under Reversed-Phase Modeeoichcdnocesest

CAPCELL PAK ADME-HR
CAPCELL PAK INERT ADME-HRB

The introduction of Adamantylethyl groups provides a hydrophobic interaction while maintaining highsurface polarity, resulting in retaining polar analytes even under water-rich mobile phases.

Physical property values
CAPCELL PAK ADME-HRCAPCELL PAK INERT ADME-HRParticle Size Pore Size Surface Area Ligand Density(m2/g) (μmol/m2)
（μm）
(nm)

C%

pH RangeMax. Pressure(MPa)2103102.7

122〜9100

3103102.7

122〜920510310

2.7

122〜920Particle Size Pore Size Surface Area Ligand Density(m2/g) (μmol/m2)
（μm）
(nm)
310310

2.7

C%

pH RangeMax. Pressure(MPa)122〜950

Structure of INERT Column

Inner Surface: PEEK
Filter: PEEK

What is ADME?

Parameter Map
0.8

ADME is an abbreviation ofAdamantane, which consists of ten
carbons in a diamond-like structure.
Ethyl groups are introduced to theAdamantane as a spacer andemployed as a unique bonded phasefor the CAPCELL PAK ADME-HRcolumns.

CAPCELL PAK ADME-HR
0.7

Surface Polarity

CN UG120

Ph UG120
AQ0.5

C8 DD

CORE AQ
MGII0.4

CORE C18

CAPCELL PAK Series
UG120
CAPCELL CORE Series
ACR
Other companies column (C18)

0.3
1.7

Adamantylethyl Groups

CAPCELL CORE ADME

0.6

1.8

1.9

2.0

UG80

2.1

2.2

2.3

Hydrophobicity(ADME Groups)

A Truly Unprecedented Balance of Hydrophobicity and Surface Polarity

Comparison of Surface Polarity between a C18 and ADME column

Comparison of Stereoselectivity■ HPLC Conditions■ ADME column■ C18 column

Column size : 2.1 mm i.d. x 50 mm
Mobile phase : H2O / CH3CN = 70 / 30
Flow rate: 0.4 mL/min
Temperature : 40 ℃Detection: PDA 220 nm
Inj. vol.
: 3 μL (50 μg/mL each)
Sample: 1. 17β-Estradiol（E2）2. Estrone（E1）3. Ethynylestradiol（EE）

OH
H
H
HOAnalyteH

C18 groups are tangled

Ethynylestradiol（EE）

C18 groups

OHH

12
31H
HO

H

Analyte17β-Estradiol（E2）
OH

23H
HO

Conventional C18 column

ADME groups
HEstrone（E1）1High-energy
sites

Highly Polar C18 column

3C

CAPCELL CORE ADME S2.70High-energy
sites

2246

［Min］

810Si

The caged-structured ADME groups offer interaction with the surfaceof the silica, resulting in providing a unique selectivity compared to aconventional C18 column.

CAPCELL PAK SERIESHighly Stable under 100 % Water Mobile Phases
As shown on the right, the efficiency is very stableeven after 600 injections under acidic mobile phase.

Results after 600 Consecutive Injections

Variation of Efficiency of Thymine

1100600
500
400300200100
Initial［Min］

600015500104005Initial050300

7 82004 5 6100Column size : 2.1 mm i.d. x 150 mm
Mobile phase : 10 mmol/L HCOONH4（Adjusted with formic acid at pH 3）
Flow rate: 0.2 mL/min
Temperature : 40 ℃Detection: UV 254 nm
Inj. vol.
: 1 μL
Sample: 1. Cytosine 2. Uracil 3. Guanine 4. Hypoxanthine
5. Xanthine 6. Oxipurinol 7. Allopurinol 8. Thymine

3

Initial Efficiency (%)

2■ HPLC Conditions

Number of Injections

Retention Behavior of Biogenic Amines under 100 % Water Mobile Phase
As shown below, CAPCELL PAK ADME-HR offers stronger retention of polar analytes, resulting in delivering complete separation for all analytes.
123

CAPCELL PAK ADME-HR S3
13.6 MPa

N6=20967

54OHN

6NH2HONO

2. Norepinephrine

N6=16031

CAPCELL PAK ADME-HR S5
8.1 MPa

H2NHO

1. Creatinine
OH
HOOH

HOHN

HOHO

H2NO

4. L-Dopa

3. Epinephrine

OHHO

1, 2HOHO

3

Highly Polar C18 5 μm column
Pressure: 7.2 MPa562

N6=12460

31Column size : 4.6 mm i.d. x 150 mm
Mobile phase : 0.1 vol% HCOOH
Flow rate: 1.0 mL/min
Temperature : 40 ℃Detector: NQAD（Evaporation 60 °C, Nebulizer 30 °C）
Inj. vol.
: 3 μL4, 562

H2N

6. Normetanephrine■ HPLC Conditions

1Hybrid ODS 5 μm column
Pressure: 7.9 MPa0

N4=12377

4O

H2N

5. Dopamine

3456

［Min］

Analysis of Nucleic-Acid Bases and Nucleosides via Gradient Elution
As shown below, the unique selectivity provided from CAPCELL PAK ADME-HR shows complete separation for all analytes, but with stronger retention of polar analytes.
2H 2N

645ONNHNH1CAPCELL PAK ADME-HR S5N

73ONH

O

1. Cytosine

NHNHO

2. Uracil

ONNH
NH

NH2

3. Guanine

NH2ON

2673Highly Polar C18 5 μm column

N1HONNH
NH2NNH2N54HH

OH

O

H
HOH

5. Guanosine

73Hybrid ODS 5 μm column

0■ HPLC Conditions

62415510［Min］O

4. Thymine

NH

NNHOH

N

6. Adenine

HOH

ONN

H
H
OH

7. Adenosine

Column size : 2.0 or 2.1 mm i.d. x 150 mm
Mobile phase : A) 10 mmol/L HCOONH4, H2OB) CH3CN
B 1 % (0 min) -> 1 % (1 min) ->40 % (15 min) -> 1 % (15.1 min) Gradient
Flow rate: 0.2 mL/min
Temperature : 40 ℃
Detection: UV 254 nm
Inj. vol.
: 2 μLOrdering Information
CAPCELL PAK ADME-HR
LengthParticle Size I.D.
（μm） （mm） （mm）

P/N

Description

93300

ADME-HR

22.193301

ADME-HR

293302

ADME-HR

93303

Particle Size I.D.
Length（μm） （mm） （mm）

P/N

Description

2093350

ADME-HR

52.1

202.15093351

ADME-HR

52.13522.110093352

ADME-HR52.150

ADME-HR

22.115093353

ADME-HR

52.17593310

ADME-HR（1/32）

30.310093354

ADME-HR

52.110093311

ADME-HR（1/32）

30.315093355

ADME-HR

52.115093312

ADME-HR

31.010093356

ADME-HR

52.1250

93320

ADME-HR

32.12093360

ADME-HR53.015093321

ADME-HR

32.13593361

ADME-HR53.025093322

ADME-HR

32.15093370

ADME-HR

54.63593323

ADME-HR

32.17593371

ADME-HR

54.65093324

ADME-HR

32.110093372

ADME-HR54.67593325

ADME-HR

32.115093373

ADME-HR

54.610093326

ADME-HR

32.125093374

ADME-HR

54.615093330

ADME-HR

33.05093375

ADME-HR

54.625093331

ADME-HR33.010093380

ADME-HR

5103593332

ADME-HR33.015093381

ADME-HR

51015093340

ADME-HR

34.63593382

ADME-HR

51025093341

ADME-HR34.65093390

ADME-HR

5203593342

ADME-HR34.67593391

ADME-HR

5205093343

ADME-HR34.610093392

ADME-HR

52010093344

ADME-HR34.615093393

ADME-HR

52015093345

ADME-HR34.625093394

ADME-HR

52025012600

ADME-HR CARTRIDGE （2PCS）

32.01012610

ADME-HR CARTRIDGE （2PCS）

52.01012601

ADME-HR CARTRIDGE （2PCS）

34.01012611

ADME-HR CARTRIDGE （2PCS）

54.01012415

CARTRIDGE HOLDER 10 （L）‒‒1012415

CARTRIDGE HOLDER 10 （L）‒‒

10CAPCELL PAK INERT ADME-HR
Particle Size I.D.
Length（μm） （mm） （mm）

P/N

Description95001

ADME-HR

32.05095002

ADME-HR

32.010095003

ADME-HR32.0

150

CONTACT US :1-12-18 Awaza, Nishi-ku, Osaka 550-0011, Japan
TEL: +81-(0)6-6110-1598, FAX: +81-(0)6-6110-1612
E-mail: silica@osaka-soda.co.jp© Copyright OSAKA SODA CO., LTD. March 2006. All rights reserved.
No part of this catalog may be copied or reprinted, for commercial
purposes, without prior written permission from OSAKA SODA CO., LTD.
2020.10 PUB