Status Report of Hyogo-Beamlines with Research Results, Vol. 5, 6 (2016, 2017)

2014A3264, 2015A3264, 2015B3264, 2016A3264, 2016B3264

BL24XUX ઢ CT Λ༻͍֤ͨࠃঁੑໟ൅ͷ‫࡯؍‬Observation of Hairs in Various Countries Using X-ray CTmethodҏ౻ ྿ɺ౉ᬒ ߛհɺલా ‫و‬ষɺླా ࿨೭
Len Ito, Kousuke Watanabe, Takaaki Maeda, Kazuyuki Suzuta‫ࣜג‬ձࣾϛϧϘϯɹதԝ‫ॴڀݚ‬Central Research Institute, Milbon Co. Ltd.,֓ཁɿɹจԽ΍ੜ‫׆‬श‫׳‬ͷҧ͏֤ࠃঁੑໟ൅ʹੜ͡Δμϝʔδ‫ݱ‬৅Λ໌Β͔ʹ͢Δ͜ͱ͸ॏཁͰ͋Δɽຊ‫ڀݚ‬Ͱ͸ɼໟ൅಺ີ౓ͷଌఆͱɼͦͷ಺෦ΛඇഁյͰ͔ͭߴ෼ղೳͰ‫͢࡯؍‬ΔͨΊʹ์ࣹޫΛ༻͍ͨ X ઢ CT ଌఆΛߦ͍ɼจԽ΍ੜ‫׆‬श‫׳‬ͷҧ͏֤ࠃঁੑͷ‫ڞ‬௨ͷμϝʔδΛ୳ࡧ͢Δ͜ͱΛ໨తͱ࣮ͯ͠‫ݧ‬Λߦͬͨɽ֤ࠃঁੑໟ൅͸ɼࠜ‫ʹݩ‬ൺ΂ͯໟઌͷໟ൅಺ີ౓͕‫ݮ‬গ͍ͯ͠Δ͜ͱΛ֬ೝ͢Δͱͱ΋ʹɼີ౓‫ݮ‬গͨ͠ໟ൅͸ɼણҡํ޲ʹ޲͔ͬͯ๮ঢ়ͷۭಎ͕ੜ͍ͯ͡Δ͜ͱΛ‫ڞ‬௨‫ݱ‬৅ͱͯ֬͠ೝͨ͠ɽߋʹɼ͜ͷ‫ڞ‬௨‫ݱ‬৅Λղফ͢΂͘ɼ‫ݪ‬ྉͷ୳ࡧΛߦͬͨͱ͜ΖγϧΫ༝དྷͷϖϓνυ͕༗ޮͰ͋Δ͜ͱ͕෼͔ͬͨɽΩʔϫʔυɿɹ X ઢ CTɼໟ൅ɼໟ൅಺ີ౓ɼԽহ඼എ‫ڀݚͱܠ‬໨తग़དྷͳ͍ܽ఺΋͋ΔɽຊใࠂͰ͸ɼ·ͣࠜ‫ݩ‬΍ໟઌͱໟ൅ʹϋϦɾίγɼ͏Δ͓͍ɼπϠ‫Ͳͳײ‬ͷ෩߹͍Λ༩͑Δ͜ͱ͸ɼ಄൅Խহ඼ʹରͯ͠ফඅऀ͔Βཁ๬͞ΕΔ‫ػ‬ೳͷ̍ͭͰ͋Δɽैͬͯɼ͜ΕΒͷ෩߹͍ΛՊֶతͳύϥϝʔλʔͰ஧࣮ʹද‫͢ݱ‬ΔͨΊͷ༷ʑͳ͍ͬͨໟ൅෦Ґͱໟ൅಺ີ౓ͷؔ܎Λ໌Β͔ʹ͠ɼͦͷ‫ޙ‬ɼີ౓͕‫ݮ‬গ͍ͯ͠Δໟ൅಺෦Λඇഁյతʹଌఆ
͢Δ͜ͱ͕ग़དྷΔ SPring-8 ฌ‫ݝݿ‬ϏʔϜϥΠϯ X ઢCT ଌఆʹͯ‫݁ͨ͠࡯؍‬ՌΛৄઆ͢Δɽ‫͞ͳ͕ڀݚ‬Ε͍ͯΔɽ֤Խহ඼ϝʔΧʔͰ͸ɼάϩʔόϧԽΛ૝ఆ༷ͨ͠ʑͳ‫ૅج‬ՊֶతΞϓϩʔν͔Βໟ࣮‫ݧ‬൅‫͕ڀݚ‬ਐΊΒΕ͖ͯͨɽͦͷ୅දతͳྫͱͯ͠ɼ֤ࠃؒʹ͓͚Δໟ൅‫ܘ‬΍ପԁ཰ͷҧ͍ [1]ɼϝσϡϥͷҧ͍ [2]ɼ‫ͼٴ‬ໟ൅ද໘Λ෴͏ΩϡʔςΟΫϧͷຕ਺ͷҧ͍ [3] ͳͲ͕‫͛ڍ‬ΒΕΔɽ͜ͷΑ͏ͳதɼզʑ͸༷ʑͳ࣮‫͔఺ࢹݧ‬Β֤ࠃঁੑͷໟ൅ղੳΛߦ͖ͬͯͨɽໟ൅಺ͷີ౓ʹؔͯ͠͸ɼ1970 ೥୅ʹߦΘΕͨύʔϚωϯτ΢Σʔϒॲཧ΍ϒϦʔνॲཧʹΑΔີ౓มԽʹؔ͢Δ‫͔ڀݚ‬Β [4, 5]ɼ͜ΕΒͷඒ༰ॲཧͰ͸ໟ൅ີ౓͸΄ͱΜͲมԽ͠ͳ͍ͱߟ͑ΒΕ͖ͯͨɽ͔͠͠࠷ۙɼචऀΒʹΑͬͯਫ਼౓ྑ͍ໟ൅಺ີ౓ͷଌఆ๏͕‫ݟ‬ग़͞Εͨ͜ͱͰ [6, 7]ɼໟ൅಺ͷີ౓มԽʹண‫͠؟‬ͨ৽ͨͳ঎඼ʹ޲͚ͨ‫͕ڀݚ‬ਐΊΒΕ͖ͯͨ [8]ɽ·ͨɼҰൠతʹ͸ɼໟ൅಺෦ͷมԽΛ‫ݟ‬Δํ๏ͱͯ͠ಁա‫ݦࢠిܕ‬ඍ‫Ͳͳڸ‬Λ༻͍ͨໟ൅அ໘‫࡯؍‬΋ߦΘΕ૬ର࣪౓ 50%, 25◦ C ʹ͓͍ͯ 1 ൩ௐ࣪͞Ε֤ͨࠃঁੑͷࠜ‫ݩ‬෦෼ʢ৽ੜ෦෼ʣͱໟઌ෦෼ʢࠜ‫ݩ‬ΑΓ
໿ 30cm ෦෼ʣͷໟ൅಺ີ౓ʢ୯Ґମੵ౰ͨΓͷ࣭ྔ:g/cm3 ʣΛଌఆͨ͠ɽໟ൅಺ີ౓Λճ෮ͤ͞Δέϥνϯͷ୳ࡧͱͯ͠͸ɼ1%έϥνϯΛௐ੔͠ɼͦͷ༹ӷʹໟ൅Λਁ௮ɼચড়ɼ‫ס‬૩‫ޙ‬ɼ૬ର࣪౓ 50%, 25◦ C ʹ
͓͍ͯ 1 ൩ௐ࣪͠ɼີ౓ଌఆΛߦͬͨ [9]ɽີ౓ଌఆΑΓ༗ҙʹີ౓‫ݮ‬গΛͱΒ͑ͨໟ൅Λ 0.2mmφ ͷΧϓτϯΩϟϐϥϦʔʹ໿ 4 ຊೖΕɼࣨԹɼେ‫ؾ‬ѹԼͰ SPring-8 ϏʔϜϥΠϯ BL24XU ʹͯଌఆΛߦͬͨɽଌఆʹ༻͍ͨ X ઢ͸ 7.0 keV ͷ୯৭ޫͰ͋Γɼ౤Ө਺໿ 1000 ຕͷը૾σʔλΑΓ CT ࠶ߏ੒Λߦ͍ɼ3 ࣍‫ߏݩ‬଄Λ‫ٻ‬Ίͨ [10]ɽ

͍ͯΔ͕ɼ͜ΕΒ͸ໟ൅಺෦Λඇഁյతʹ‫ݟ‬Δ͜ͱ͕

– 20 –ฌ‫ݝݿ‬ϏʔϜϥΠϯ೥ใɾ੒Ռू
ઌ෦෼ͷໟ൅಺ີ౓Λࣔͨ͠ɽໟ൅ͷ৳௕͸Ұ೔Ͱ໿

0.4 mm ͱ͍͏ใࠂ͔Β [11]ɼࠜ‫ݩ‬ΑΓ 30 cm ઌͷໟઌ෦෼ͱ͸ɼ໿ 2 ೥ؒʢ໿ 0.4 mm × ໿ 730 ೔ = ໿292 mmʣʹͳ͞Εͨඒ༰ࢪज़΍ੜ‫׆‬श‫ʹ׳‬Αͬͯੜͨ͡μϝʔδΛ஝ੵͨ͠΋ͷͱߟ͑ΒΕɼͦͷໟઌ෦෼͸ɼͲͷࠃʹ͓͍ͯ΋ࠜ‫ݩ‬෦෼ΑΓີ౓͕௿͘ͳͬ
͍ͯΔ͜ͱ͕໌Β͔ʹͳͬͨɽ͜ΕΒີ౓͕‫ݮ‬গ͢Δ͜ͱʹΑͬͯɼໟ൅಺෦͕ͲͷΑ͏ʹมԽͨ͠ͷ͔ՄࢹԽΛࢼΈͨɽ(ii) ໟ൅಺෦ߏ଄ͷධՁ ∼ X ઢ CT Λ༻͍ͨ‫ݕ‬౼ʢSPring-8 BL24XUʣ∼Fig. 1. Density of root and tip hairs of each region.
Root, the proximal root part of hair; Tip, at about
30 cm from the proximal root part of hair.

ໟ൅಺ີ౓มԽ͕Կʹ‫ى‬Ҽ͍ͯ͠Δ͔ߟ࡯͢ΔͨΊʹɼಁա‫ݦࢠిܕ‬ඍ‫ڸ‬ʢTEMʣΛ༻͍ͯໟ൅ͷԣஅ໘੾ยͷ‫࡯؍‬Λߦͬͨ [12]ɽͦͷ݁Ռɼີ౓Λࣦͬͨໟ൅಺෦ͷۭܺ਺͕૿Ճ͍ͯ͠Δ͜ͱ͕൑໌ͨ͠
ʢdata not shownʣɽ͔͠͠ɼԣஅ໘੾ยΛ࡞੒͢Δ݁Ռ͓Αͼߟ࡯ࡍͷΧοςΟϯάӨ‫Ͳͳڹ‬ɼഁյૢ࡞࣌ʹ͓͚ΔଛইͷՄೳੑΛ১͍‫ڈ‬Δ͜ͱ͕Ͱ͖ͳ͔ͬͨͨΊɼඇഁյ(i) ֤ࠃໟ൅ͷີ౓มԽ
Fig. 1 ʹ֤ࠃʹ͓͚Δࠜ‫ݩ‬෦෼ʢ৽ੜ෦෼ʣͱໟ๏Ͱ͋Δ X ઢ CT ๏Λ༻͍ໟ൅ͷ಺෦ߏ଄Λ‫͠࡯؍‬ͨɽໟ൅ͷ಺෦ߏ଄ΛಘΔͨΊʹ͸ߴ͍ۭؒ෼ղೳΛ

Fig. 2. 3D images of root and tip hairs of each region using X-ray CT method at SPring-8 (BL24XU). TheRoot, for the most similar image in each region, showed one typical example. At 0 and 15 degree angle tofiber axis including cuticles layer. At 90 degrees angle to fiber axis. The Stick Shaped shadows in the hairare voids.

– 21 –

Status Report of Hyogo-Beamlines with Research Results, Vol. 5, 6 (2016, 2017)
ඞཁͱ͢ΔͨΊɼSPring-8 BL24XU (ฌ‫ ݝݿ‬ID) Ͱར༻Մೳͳ์ࣹޫ݁૾̭ઢ CT ૷ஔΛ༻͍ͨɽຊ CT૷ஔ͸ޫֶ‫ʹܥ‬ϑϨωϧκʔϯϓϨʔτʹΑΔ֦େ݁૾‫ݦ‬ඍ‫ڸ‬Λ࠾༻͓ͯ͠ΓɼσϑΥʔΧεଌఆʹΑΓιϑτϚςϦΞϧࢼྉΛαϒϛΫϩϯͷۭؒ෼ղೳͰ‫؍‬࡯͢Δ͜ͱ͕Ͱ͖Δɽͦͷ݁Ռɼ֤ࠃঁੑͷໟઌ෦෼ͷໟ൅͸ͦͷࠜ‫ݩ‬෦෼ʹൺ΂ɼۭܺͷ૿Ճ܏޲Λ‫؍‬ଌͨ͠ʢFig. 2ʣɽۭܺ͸ɼણҡํ޲ʹ๮ঢ়ͷ‫ܗ‬Λ༗͓ͯ͠Γɼզʑ͸͜ͷ‫ݱ‬৅Λ Stick Shape Voids (SSV)ͱ‫ݺ‬ΜͰ͍Δɽ͜ͷΑ͏ʹໟઌ෦෼Ͱ๮ঢ়ͷ‫ܗ‬Λ͍ͯ͠Δ͜ͱʹΑΓɼໟઌ෦෼Ͱࢬໟ͕‫͜ى‬Γ΍͔ͬͨ͢ΓɼۭܺͷӨ‫Ͱڹ‬πϠͳͲͷ௿Լ͕‫͜ى‬Δͱߟ͍͑ͯΔɽ‫ࡏݱ‬ͷͱ͜Ζɼଛই෦Ґͷಉఆʹ͸ࢸ͍ͬͯͳ͍

Fig. 3. Hair density enhancing component screening.

͕ɼ෼ղೳΛߋʹ޲্͢Δ͜ͱͰɼଛইϝΧχζϜΛ

Results of the density measurement after treating໌Β͔ʹ͍͖͍ͯͨ͠ͱߟ͍͑ͯΔɽ

each of the components. A-C are various kinds ofpeptides. D is a peptide derived from silk.

(iii) SSV Λճ෮Ͱ͖Δ੒෼ͷ‫ݕ‬౼͜Ε·Ͱɼզʑ͸ SSV ͷओཁͳ‫ݪ‬Ҽ͕ໟ൅λϯύΫ࣭ͷྲྀग़ʹΑͬͯ‫͜ى‬Δ͜ͱΛใࠂ͍ͯ͠Δ [13]ɽ
͜ͷ SSV Λճ෮ͤ͞Δ΂͘ɼ1%έϥνϯΛ༻͍ͯີ౓Λճ෮͢Δ͜ͱͷͰ͖ΔέϥνϯΛ୳ࡧͨ͠ͱ͜ΖɼγϧΫ༝དྷͷϖϓνυ͕།Ұີ౓Λճ෮ͤ͞Δ͜ͱ͕Ͱ͖Δ͜ͱΛ֬ೝͨ͠ʢFig. 3ʣɽ·ͨɼ͜ͷΑ͏ʹໟ൅಺ີ౓͕ճ෮ͨ͠ໟ൅Λ༻͍ͯ X ઢ CT ଌఆͰ‫͜ͱͨ͠࡯؍‬Ζɼໟ൅಺ʹੜ͍ۭͯͨܺ͡ͷ‫ݮ‬গΛଊ͑Δ͜ͱ͕Ͱ͖ͨʢFig. 4ʣɽ͜ͷΑ͏ʹɼγϧΫ༝དྷͷϖϓνυ͸͋ΒΏΔμϝʔδʹΑͬͯໟ൅಺
ʹੜۭͨܺ͡ΛຒΊΔ͜ͱͰ͖Δ੒෼Ͱ͋Δ͜ͱΛ֬

Fig. 4.

3D images of 0.01% Silk-derived peptide

treated hair before and after using X-ray CT method
at SPring-8 (BL24XU).

ೝͨ͠ɽࣄ‫΁ۀ‬ͷߩ‫ݙ‬·ͱΊͱࠓ‫ޙ‬ͷ՝୊ຊ‫ڀݚ‬੒ՌʹΑͬͯ‫ݟ‬ग़͞ΕͨγϧΫ༝དྷͷϖϓνຊߘͰ͸ɼ20ϲࠃͷঁੑໟ൅ͷ‫ڞ‬௨ͷμϝʔδ‫ݱ‬৅Λଊ͑Δ͜ͱΛ໨తʹ࣮‫ݧ‬Λߦͬͨɽͦͷ݁Ռɼໟ൅಺ີ౓͕௿Լͨ͠ໟ൅ʢໟઌ෦෼ʣͷໟ൅Λ X ઢ CTʹΑΓ಺෦ߏ଄Λ‫݁ͨ͠࡯؍‬Ռɼۭܺ਺ͷ૿ՃΛ֬ೝͨ͠ɽߋʹɼ͜ͷۭܺΛຒΊΔͨΊͷ୳ࡧͱͯ͠ີ౓
ଌఆɼX ઢ CT Λ༻͍ͯ֬ೝΛߦͬͨ݁ՌɼγϧΫ༝དྷͷϖϓνυ͕༗ޮͰ͋Δ͜ͱΛ֬ೝͨ͠ɽଟ༷Խͨ͠‫ސ‬٬ͷχʔζʹԠ͑Δ΂͘ɼ࠷ઌ୺ͷ෼ੳ΍‫ܭ‬ଌٕυ͸ɼฐࣾओྗϒϥϯυ ʡmilbon ʡͷ‫ڞ‬௨੒෼ͱͯ͠഑߹͞Εɼੈք 8 ͔ࠃͰ‫ൃࡏݱ‬ചΛ͞Ε͍ͯΔ [14]ɽຊ‫ڀݚ‬ΑΓ‫ݟ‬ग़͞Εͨ๮ঢ়ۭಎԽ͸ࠃ಺ͷϝσΟΞ࿐ग़ [15,16]ɼߋʹ࠷ઌ୺ͷٕज़ʹΑͬͯՄࢹԽͨ͠ը૾͸ɼւ֎ͷඒ༰ࢣͳ͍͠Ұൠ‫ސ‬٬ʹର͠ɼࢹ֮తͰ෼͔Γ΍͍͢ͱ޷ධͰɼࠃ಺֎Ͱ஫໨ΛूΊͨ‫಺ڀݚ‬༰ͱͳ͓ͬͯΓɼ঎඼Ձ஋ͷ޲্ʹͭͳ͕ͬͨͱߟ͍͑ͯΔɽज़Λ‫͢࢖ۦ‬Δ͜ͱͰ৽ͨͳ‫ݱ‬৅Λղ໌͠ɼ੡඼ͷ։ൃʹԠ༻͍͖͍ͯͨ͠ɽ

– 22 –ฌ‫ݝݿ‬ϏʔϜϥΠϯ೥ใɾ੒Ռूँࣙࠓճͷ‫ڀݚ‬Λ਱ߦ͢Δʹ͋ͨΓɼฌ‫ཱݝݿ‬େֶ࢈ֶ࿈‫ܞ‬ɾ‫ڀݚ‬ਪਐ‫ޫࣹ์ߏػ‬φϊςΫηϯλʔͷ࣫‫ྑݪ‬ণ‫ڀݚ‬һɼ౦๺େֶɹߴ໺ल࿨।‫ڭ‬तʹ͸ଌఆʹؔͯ͠ଟେͳΔࢧԉΛ͍͖ͨͩ·ͨ͠ɽ͜ͷ৔ΛआΓͯ‫ޚ‬ྱਃ্͛͠·͢ɽࢀߟจ‫ݙ‬[1] H. Otsuka and T. Nemoto: Koshokaishi 12,192-197 (1988).
[2] B. M. Das: Morph. Anthorop. 63, 102-109(1971).
[3] S. Hayashi et al.: Biology and disease of theHair, 555-561 (1976).

[4] N. Yin et al.: J. Soc. Cosmet. Chem. 28, 132(1977).
[5] C. R. Robbins: Chemical and Physical Behaviorof Human Hair p.482 (2006).
[6] L. Ito et al.: J. Hair Sci. 115, 3-6 (2015).
[7] L. Ito et al.: Fragrance J. 41(11), 18-22 (2013).
[8] L. Ito et al.: SPring-8 Research Frontiers 2014,94-95 (2015).
[9] ಛ։ 2016-61664.
[10] H. Takano et al.: Jpn. J. Appl. Phys. 52, 040204(2013).
[11] C. Saint Olive Baque et al.: Int. J. Cosmetic
Sci. 34, 111-116 (2012).
[12] I. J. Kaplin et al.: Cosmet. Toil. 97(8), 22-26(1982).
[13] ౉ᬒߛհ ଞ: ೔ຊ์ࣹޫֶձࢽ 28, 210-213(2015).
[14] ϛϧϘϯϗʔϜϖʔδ:
http://www.milbon.co.jp/brand/detail/48[15] 2016.2.1 Խֶ޻‫ۀ‬೔ใʮੈքϒϥϯυ্ཱͪ͛ʯ
[16] 2016.4.19 ೔ຊ‫৽ࡁܦ‬ฉʮϛϧϘϯɼໟ൅಺ͷີ౓ߴΊΔ੒෼഑߹ɹϔΞέΞࡎൃചʯ– 23 –