Multi Bio System
Bioprocess Equipment Division

SATAKE
BIOREACTORSERIESSatake Cell Culture Device General Catalogue

Microbial Culture / Algae Biomass
Animal Cell Culture
Regenerative Medicine / iPS Cell Culture / Stem Cell Culture

Next Stage

CAT.No. M-81E(105)The key word is “Industrialization”.
Satake proposes the most optimal “Cell Culture”
from laboratory scale to production.

Outsourcing Service for Cell Culture
Outsourcing Service for Numerical Calculation using CFD■ Feature of Satake Outsourcing Service for Cell Culture

For 30 years since the establishment of our Mixing Technology Laboratory,

We are fully equipped with a clean room (cell culture room) dedicated to contracted

Satake has been put endless efforts in mixing research and development,

cell culture in our mixing technology laboratory in order to comply with the various

mainly focusing on “Flow and its Effect”. By applying these technologies

demands of the customers including the “Inability to reproduce the results obtained

to cell culture field, we succeeded in developing novel bioreactor thatin the laboratory when scale-up is performed”, “Failure to understand what to do evencan create the most optimal environment for cell culture. As the top

though optimization in production has been examined earlier”, and “Desire to confirm

manufacturer of mixer with extensive knowledge and experience in mixing

if buying a new BioReactor would really be effective”, etc. Also, through cooperation

technology, we confident that our products will satisfy our customers. In

with external subcontractors, we are now in possession of equipment with which we

addition, we have also built schemes to customize order-made bioreactor

can examine scale-up up to the max. 200 L class, equipment and fields with which weand cell culture systems by working together hand-in-hand withcan evaluate iPS cell differentiation induction, and at the same time, we can perform

customers. Try it out as we are looking forward to serve you!

operation with the combined use of CFD simulation and contracted numerical fluidLine upcalculation, and provide optimum services in line with customers’ needs through ourknowledge of the mixing technology cultivated for many years.

Microbial Culture

P.4~7

HSF Reactor

P.8~9

Animal Cell Culture

MRF Reactor

Animal Cell Culture

P.10~11

MRF-RB Reactor■ Introduction of Equipment Owned【Counting of number of cells】【BioReactors】

・Blood cell counting chamber

・VMF reactor Liquid volume: Approx. 0.2 L to 8 L・MRF reactor Liquid volume: Approx. 1 L to 6 L
Two units can run simultaneously
・S-BOX(Controller)dissolved oxygen concentration(DO)、pH control
・SPG membrane sparger / sintering sparger
Rotating Type

Rotating Type
Animal Cell Culture

P.12~13

VMF Reactor

Animal Cell Culture

Rotating Type

P.14~15

VerSus Reactor®

Regenerative Medicine【Other】
P.16~17

VMF-WSUB Reactor/TCS

・CO2 incubator (two units) with shaker
・Centrifuge
・Clean bench・Cell counter (TC20)【Component analysis in culture supernatant】
・Multi-function biosensor (BF-7)
Constituents such as glucose, lactic acid, glutamine, glutamic acid,ammonia, and others need to be discussed.
・Absorbance microplate reader (Multiskan GO)
Lactate dehydrogenase (LDH) activity (The quantity of antibody
needs to be discussed)

Flow contracted culture

・High-pressure steam sterilizer
・Refrigerator-freezer (-20℃、5℃ )・Deep freezer (-80℃ )・Liquid nitrogen storage container
・Digital microscope (4 to 20 times)① Prior meeting・Confirmation of customer’s objective, cells, and culture method
・Explanation of equipment② Quotation

・Optical microscope (4 to 20 times)
Vertical Motion Type/
SPG Membrance Sparger

Vertical Motion TypeRegenerative Medicine /iPS Medicine Development

HiD ４×４

P.18~19

Animal Cell Culture/
Regenerative Medicine

P.20~23

Single-use Bio Reactor■ Cell handling result
Vertical Motion Type
Option partss【Cell culture of floating cells】

P.24~31

・CHO cells (Hamster ovary cells)③ Conclusion of an NDA・Disclosure of detailed information, such as protocols, etc.
・Meeting about cell culture conditions④ Grant of cell line

CHO-S、CHO-K1、CHO-DG44、CHO 1-15500
・HL60 cells (Human acute myelogenous leukemia)
・U937 cells (Human histiocytic lymphoma)【Cell culture of attached cells】
・HeLa cells (Human cervical cancer)
・Vero cells (African green monkey’s kidneys)
・MDCK cells (Canine kidneys)⑤ Cell culture experiment・Counting of number of cells by sampling, analysis of cell culture
liquid constituents・If necessary, the cells and culture supernatant are sent after cell
culture⑥ Result report・Submission of report, meeting about future course of action【Various microbial culture】
Vertical Motion Type2

Rotating Type / Vertical Motion Type

Please contact our Bioprocess Equipment Division for more information.

Contact number +81-48-471-9202 E-mail：bio@satake.co.jp

3

Plant cell culture / M ic r o bial c u ltu r ePlant cell culture A/ nMi mi c ar ol bcei al l c u l t u r e

HSF Reactor

SATAKE Sparger

High-performance BioReactor with the high-efficiency turbine HS100 / high-discharge axial
flow impeller HR100 as a standard

Further efficiency improvement is achieved by examining the whole equipment.
We will customize and provide all technologies that assume scale-up.

High-Efficiency Turbine, In-Tank Coil Heat Transmission Specs, Dedicated for Actual Machine Scale-up
The HSF Reactor is a jar fermenter designed for comparatively
low-viscosity microbe cultures, plant cell cultures, fungalbased cultures with high viscosity that show non-Newtonian
properties, E. coli cultures, etc., in which target cells andbacterial cells have a comparatively strong shear factor,
and cultures that require high OTR. It comes with the highefficiency turbine HS100 and high-discharge axial flow impeller
HR100 as a standard. Optimization is possible for scaling up
to large-capacity tasks when paired with the S-BOX series
controller for culture control.
An optional cooling chiller can be attached, and when using
a heat transfer coil type similar to the actual machine, you
can achieve conditions that are closer to the actual machine.
As a specialty manufacturer of mixing device with the only
dedicated mixing technology laboratory in Japan, we canapply final optimizations for actual machine and customize thedevice according to the physical properties of its contents.

Satake Sparger
10 3

JPN Pat.No.6189202

6-bladed flat turbine （Conventional model sparger）)
oxygen transefer capacity coefficient (■ Cooling water circulation(option)The Satake Sparger is designed with gas discharge direction
in mind so that aerated gas passes near the impeller, which iswhere the shearing field is located. Also, a stationary impeller is
attached to the ring, and increasing the velocity gradient of theflow improves shearing and destructive action. Furthermore, thestationary impeller strengthens the discharge action. These actions
efficiently disperse gases.

HS100+HR100（Conventional model sparger）
HS100+HR100（SATAKE sparger）

10 2

SATAKE Sparger

Conventional Sparger
10 1
10 -1

10 0
Volume of gas flow (200kL-F culture tank simulation results
Gas-liquid multiphase flow gas fraction analysis

10 1)

The Satake Sparger is effective when paired with the HS100. The synergistic effect with the optimized mixing impeller significantly improves the gas

This is a simple and inexpensive
control BOX that provides DO
control/ph control using ON/OFF
control. Please select dependingon your purpose.

dispersion action, increasing gas absorption performance. (According to Satake Chemical test results)■ S-BOX × 10 MC / S-BOX × 10 Simple
Item
Name
Model
Control

Culture controller
S-BOX × 10 MC
pH, DO (Dissolved oxygen), FL (O₂ flow rate), FL (Air flow rate), Pump
pH (Hydrogen ion concentration)

JPN Pat. No.3919262

Specifications

Name

HSF Reactor

Model

HSF-3
HSF-10Band heater (PID control) with over-temperature protection function (Max. 80 [℃])

Temp. control device

0.00 - 20.00 [mg/L]

Display accuracy: ±0.5 [%]accuracy

FL（O2 flow rate）

0.4 - 10.0 [L/min]

F.S.

FL（AIR flow rate）

0.4 - 20.0 [L/min]

pH (Hydrogen ion concentration)

0.00 - 14.00 [－]Power transmission
Gas supply type

Ring sparger (option : SATAKE sparger)*1

Performance

Temp. control range
Temp. accuracy

Rotational speed range
Temp. setting

Function

Speed setting

Configuration

Power of band heater
Power of motor

Mixing impellers
Culture tankDimensions

Culture operation volume

Usage condition

Outer dimensions
WeightPower supply

Room temperature + 5 - 20 [℃] (normally set to 37 [℃])±0.3 [℃]（37 [℃]）

5 - 1500 [min-1]
Touch panel input, data output DC1 - 5 [V]Touch panel input, data output DC0 - 10 [V]Max. 160 [W]

Max. 480 [W]

Max. output 100 [W]

Max. output 400 [W]

Super-Mix HS100 turbine + HR100 impeller

I.D. 140 x Depth 203 [mm]2.4 [L]

W360×D485×H905 [mm]Approx. 30 [kg]AC100 [V], 50/60 [Hz]*1 The performance is the value under room temperature 20 [℃], power supply AC100 [V], 50 [Hz] and no load condition.

4

Approx. 34 [kg]FL（O2 flow rate）

0.4 - 10.0 [L/min]

FL（AIR flow rate）

0.4 - 20.0 [L/min]

DO (Dissolved oxygen)

FL（O2 flow rate）
FL（AIR flow rate）

Sensors

0.00 - 10.00 [mg/L]

concentration)

ON/OFF control

concentration)

DC0 - 5 [V]Accuracy: ±0.5 [%] F.S.

With data logger
DC0 - 5 [V]DC0 - 10 [V]Rotational speed

DC0 - 10 [V]DC1 - 5 [V]Temperature sensor

DC1 - 5 [V]

SUS304 (no coating), indoor type, non-waterproof, non-explosion proof specification

SUS304 (no coating), indoor type, non-drip proof, non-explosion proof specification

Indoor tabletop typeW260×D320×H400 [mm] ・
5 - 45 [℃]

Humidity

Indoor tabletop type

Approx. 15 [kg]20 - 85 [%] RH (No condensation)

W260×D300×H350 [mm] ・Temperature

Polarographic DO sensor/pH sensor manufactured by Mettler Toledo company

O2
CO2
AIR (for pH)
AIR (for DO)

connection port φ6 one touch tube fitting
Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting
Flow rate 5 [L/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting
Flow rate 20 [L/min] or lower (controlled with mass flow controller),
supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting*All of O₂, CO₂, and AIR must be dry and clean gasesthat do not contain corrosive components, dust, and oil mist.

5 - 45 [℃]

Humidity

Approx. 12 [kg]20 - 85 [%] RH (No condensation)

Optical DO sensor manufactured by Automatic System Research Co., Ltd.

(Option: Optical DO sensor manufactured by Automatic System Research Co., Ltd.)
AC100 [V], 50/60 [Hz], electrical outlet 2 gang (for main control unit and recorder)
Flow rate 10 [L/min] or lower, supply pressure 0.2 [MPa],

Accuracy: ±0.5 [%] F.S.

DO (Dissolved oxygen)

Rotational speed

Power supply

Utilities

0.00 - 14.00 [－]Temperature sensor

TemperatureF.S.

pH (Hydrogen ion
With data logger

AF (antifoam)Outer dimensions/weight

Display accuracy: ±0.5 [%]0.00 - 20.00 [mg/L]

DO (Dissolved oxygen)

DO (Dissolved oxygen)

Usage conditions

0.00 - 14.00 [－]pH (Hydrogen ion
ON/OFF control

DO (Dissolved oxygen)
AF (antifoam)

MTA of outer surface
Installation

DO (Dissolved oxygen)concentration)

pH (Hydrogen ion concentration)

Data output

concentration)

pH (Hydrogen ion

concentration)

Control type

6 [L]

W360×D485×H980 [mm]0.00 - 10.00 [mg/L]

pH (Hydrogen ion

I.D. 200 x Depth 360 [mm]10 - 35 [℃]

DO (Dissolved oxygen)

Culture controller
S-BOX×10 Simple
pH, DO (Dissolved oxygen)

pH (Hydrogen ion

DO (Dissolved oxygen)

Setting range+ （option : In-vessel coil heat exchanger）
Magnet drive (non-sealing type)0.00 - 14.00 [－]Display range/■ HSF-Reactor
Item

Specifications+ pH sensor manufactured by Mettler Toledo company
Power supply

O2
CO2
AIR

AC100 [V], 50/60 [Hz], electrical outlet 2 gang (for main control unit and recorder)
Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting
Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting
Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting*All of O₂, CO₂, and AIR must be dry and clean gasesthat do not contain corrosive components, dust, and oil mist.

System development and customization for demand specification are also possible.
Please contact our Bioprocess Equipment Division below for more information.
Contact number +81-48-471-9202 e-mail address：bio@satake.co.jp

5Plant cell culture / M ic r o bial c u ltu r ePlant cell culture A/ nMi mi c ar ol bcei al l c u l t u r eHigh performance turbine impeller and axial flow impeller are standard specifications

Example of 200 kL Actual Machine CFD Simulation Analysis

Further efficiency improvement is achieved by examining the whole equipment.We will
customize and provide all technologies that assume scale-up.

HS100 turbine
The HS100 turbine achieves an extremely low power number (Power
number ratio to 6FT: approx. 65% reduction). Liquid fluidizing action
is improved compared to conventional turbines, along with highperformance gas absorption (KLa). Impeller lift at low power creates
more focused flow, utilizing pressure gradients and fluctuations of
the discharge field to produce powerful shearing and destructive
action. When paired with the high-discharge type HR100 Impeller,
this impeller achieves overall incredibly highly efficient in-tank liquid
fluidizing action, shearing, and destructive action (gas dispersion).

ɻ
( K La ) [hr-1]
ɻ

■ Comparison of gas absorption performance (kLa)
water-air systemɻQ=1.0 [vvm]When using the conventional 6-bladed flat turbine in multi-stage within a culture tank, the flow is divided creating conditions
that are not favorable to uniform dispersion inside the culture tank. With this in mind, a new, high-performance BioReactor
is created by utilizing the flow action in the axial direction of high-discharge axial flow impeller HR100 on the top of the tankin combination with high-dispersion turbine HS100 on the bottom of the tank for gas dispersion action, resulting in uniform
dispersion inside the culture tank and high-performance gas absorption. HSF reactors are suitable for lab-scale considerationfrom 3 to 10 L, and can also be scaled up to production machines (large capacity) at the same requirements.

6-bladed flat turbineʤ6FTʥ
Cone cave turbineʤ6CCʥ
HS100 turbine

10 2

approx.

0.5

1.5

6 flatHS100

turbineturbine

The results of shear stress analysis in the vicinity of the blade under
10 1
10 -1

10 0

10 1

Power of aeration mixing ( P gv ) [kW/m3]

the same-energy (Pv-value) conditions show that the shear stress ofSuper-mix® HS124ND, HS134ND Turbine
Impellers to enhance the performance of gas

HS100 is higher than 6FT in the discharge field.

HR100 Impeller

High Discharge Axial Flow impeller for Ultimate Homogenization in Culture Tank.
(combined with High-Efficiency Turbines)■ Flow pattern of the HR100 Impeller■ Discharge performance by LDV(compared with conventional 4PP model)

6

Discharge flow rate （（In addition to considerations regarding the plane
shape of the impeller, the angle of attack, andthe camber ratio, the HR100 Impeller featuresa multistage curved structure, and suppressing
separation on the rear surface of the impeller
results in an energy-saving, low-shear typeaxial flow impeller that boasts high discharge
performance at low power. This product excels
at liquid-liquid mixing, solid-liquid dispersion,
uniform suspension of particles that are fragileand lightweight, emulsified micro-capsules (latex,etc.), and is effective in combinations that utilize
superior axial fluidizing action in multi-stage
mixing.

Satake model: HR100 double stage + HS100 single stage

Gas dispersion comparison results via multiphase flow model (Euler-Euler)of 200 kL culture tank in CFD simulation■ CFD flow analysis result near the blade (blade cross section)

10 3

approx.

Conventional model: 6-bladed flat turbine triple stage

4-blade pitched paddleʤ4PPʥ
HR100 Impeller

0.55

HS124ND

HS134NDJNP Patent No. 6159577

JNP Patent No. 6159577

Ultra high-efficiency turbines HS124ND/HS134ND
boast gas absorption performance that exceeds eventhat of the high-efficiency turbine HS100. The upperand lower impellers create effective discharge action,achieving a high level of gas absorption performanceand required OTR. Technology that delivers the world's
highest gas absorption performance and required OTR
is available at laboratory scale in 200 - 300 kL-classactual machines.

Performance History of Large-size Culture Tank
SATAKE takes pride in delivering many culture tanks from small size to large
size. Here, we introduce an example of delivery records of large-size cell
culture reactors (100 kl to 300 kl class).

0.25

0.00
0.0

0.6

1.2

Measurement positionɻʪ（d/2）/ d ʫ[-]

【Test conditions】
Tank dia.：DImpeller dia.：dRotation speed：N
Vertical axis：Discharge velocity( Vq) / Blade tip
velocity(Vtip)
Horizontal axis：Impeller radius
D=490mm d/D=0.3 N=300min-1

If you want to order a culture tank as well, the same will be jointly handled by
the tank manufacturer working together.
【Motor power】
・600kW・480kW・ 130kW・490kW・470kW・ 110kW

・485kW・ 430kW・ 90 kWetc.
System development and customization for demand specification are also possible.
Please contact our Bioprocess Equipment Division below for more information.
Contact number +81-48-471-9202 e-mail address：bio@satake.co.jp

7Animal cell culture / M ic r o c ar r ier cu l t u r e

An i m a l c e l l c u l t u r e / MAi nc ri mo caalr cr ieelrl c u l t u r e

MRF Reactor

An environment that is best suited for cell culture is created through excellent mixing performance.

Rotating type mixer for cell culture equipped with an MR210Bio impeller as astandard

CFD Simulation Analysis Result■ Flow condition inside culture tank

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications■ Mixing impeller used
exclusively for Cell Culture

Super Mix® MR210 Bio
JNP Patent No. 4187579

The MRF Reactor is a BioReactor for laboratory use that aim
at actual production and scaling up. In addition to a “Simple
shape with excellent cleaning ability” that is important for theactual equipment, the “MR210Bio impeller used exclusively forcell culture” that exhibits excellent mixing and homogenizing
performance is provided as a standard, and therefore, similar
culture results as during laboratory experiments can be
reproduced during scale-up too. Moreover, fluid surface
fluctuation (fed-batch culture) is supported, because of which
the same mixing performance is exhibited no matter how
much the liquid volume. A wide range of liquid volumes fromsmall volumes like 1.5 L to large volumes of 20,000 L can besupported.
A dedicated controller “S-BOX × 10 α Ⅱ ”which can control
DO,pH is provided. Moreover analog singnals can be input todata logger and record them.

The “MR210Bio” impeller developed exclusively for cell culture
produces a high circulating flow that runs from the low-pressurearea at the bottom of the blades, where the mixing homogeneity
inside the tank is high, toward the top of the tank. As a result of
this feature, a high homogeneity and fluidity are obtained even at
low power and low rotation, and at the same time, the basic flow
pattern does not change even when the fluid surface fluctuates. It
is known as an impeller used exclusively for cell culture that adapts
to unsteadiness, which was not seen in the past in any impeller,
and is suitable for operation under conditions where the fluid
surface fluctuates as a result of fed-batch culture. A high circulation
performance can be seen from the CFD simulation analysis results.

Flow pattern of MR210Bio impeller

Comparison of the cell viability in the later stage of■ Culture example

the culture by CHO cells

O pt i on Simplified controller
cell viabillity [%]This is a simple and inexpensive
control BOX that provides DO
control/ph control using ON/OFF
control. Please select dependingon your purpose.

Here we show the cell viability in the later stage of the culture
under the batch cell culture condition for the turbine type (flatturbine) and wide paddle type (elephant ear) impellers used in
the conventional rotating type BioReactor. It can be confirmedthat the MRF reactor maintains a higher cell viability than theother conventional impellers in the later stage of the culture. A
characteristic of the MR210Bio that has a low shearing action andhigh mixing homogeneity performance is the realization of scaleup to a large-size reactor through the combined use of numerical
fluid calculation.

cell culture time [hr]JPN Pat. No.4187579■ S-BOX × 10 α II / S-BOX × 10 Simple

Specifications

Item■ MRF-Reactor

Item

Specifications

Name

MRF Reactor

Model

MRF-3

MRF-8 (10 [%] Dish bottom head)

MRF-10Temp. control device

Band heater (PID control) with over-temperature protection function (Max. 80 [℃])Power transmission

Magnet drive (non-sealing type)Gas supply type

Shirasu porous glass (SPG) membrane type / sintered metal type (selectable)*1

Performance

Temp. control range

Room temperature + 5 - 20 [℃] (normally set to 37 [℃])

Temp. accuracy±0.3 [℃]（37 [℃]）-1

Rotational speed range
Function

Configuration

Culture tank

Display range/accuracy

DO (Dissolved oxygen)

Touch panel input, data output DC1 - 5 [V]Speed setting

Touch panel input, data output DC0 - 10 [V]

MTA of outer surface
Installation

Max. 160 [W]

Max. 480 [W]

Power of motor

Max. output 100 [W]

Mixing impeller

Super-Mix MR210Bio impeller

Max. 480 [W]

I.D. 140 x Depth 203 [mm]I.D. 200 x Depth 330 [mm]I.D. 200 x Depth 360 [mm]Culture operation volume

2.4 [L]6 [L] (10 [%] Dish bottom head)

7 [L]Usage condition

10 - 35 [℃]

Outer dimensions

W360×D485×H905 [mm]WeightPower supply

Approx. 30 [kg]Approx. 34 [kg]AC100 [V], 50 /60 [Hz]*1 The performance is the value under room temperature 20 [℃], power supply AC100 [V], 50 [Hz] and no load condition.

Outer dimensions/weight
Usage conditions

Sensors

Dimensions

DO (Dissolved oxygen)

Control type

Temp. setting

FL (O2 flow rate)
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
FL (O2 flow rate)
Rotational speed
Temperature sensor

0.00 - 20.00 [mg/L]

pH (Hydrogen ion concentration)

F.S.

DO (Dissolved oxygen)

0.00 - 20.00 [mL/min]
0.00 - 14.00 ［－]0.00 - 10.00 [mg/L]
0.00 - 20.00 [mL/min]
ON/OFF control
O2 addition/subtraction step control
O2 and N2 ON/OFF control
O2 PI control (slope set method: TIME, %)PI control (slope set method: TIME, %)With data logger
DC0 - 5 [V]Accuracy: ±0.5 [%] F.S.

DC0 - 10 [V]DC1 - 5 [V]pH, DO (Dissolved oxygen)

Display accuracy: ±0.5 [%]Indoor tabletop type

pH (Hydrogen ion concentration)
DO (Dissolved oxygen)

Temperature

5 - 45 [℃]

Humidity

20 - 85 [%] RH (No condensation)

0.00 - 14.00 [－]Display accuracy: ±0.5 [%]F.S.

0.00 - 20.00 [mg/L]
0.00 - 14.00 [－]0.00 - 10.00 [mg/L]

pH (Hydrogen ion concentration)
ON/OFF control
DO (Dissolved oxygen)
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
Rotational speed
Temperature sensor

With data logger
DC0 - 5 [V]DC0 - 10 [V]DC1 - 5 [V]Accuracy: ±0.5 [%] F.S.

SUS304 (no coating), indoor type, non-waterproof, non-explosion proof specification

Indoor tabletop type

W350×D400×H530 [mm] ・ Approx. 15 [kg]W260×D300×H350 [mm] ・Temperature

Polarographic DO sensor/pH sensor manufactured by Mettler Toledo company

5 - 45 [℃]

Humidity

Approx. 12 [kg]20 - 85 [%] RH (No condensation)

Optical DO sensor manufactured by Automatic System Research Co., Ltd.

(Option: Optical DO sensor manufactured by Automatic System Research Co., Ltd.)+ pH sensor manufactured by Mettler Toledo company

Power supply AC100 [V], 50/60 [Hz], electrical outlet 2 gang (for main control unit and recorder)
Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa],

Power supply AC100[V], 50/60 [Hz], electrical outlet 2 gang (for main control unit and recorder)
Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa],

O2Approx. 34 [kg]S-BOX×10 Simple

SUS304 (no coating), indoor type, non-waterproof, non-explosion proof specification

N2

Utilities

Culture controller

S-BOX×10αII
pH, DO (Dissolved oxygen), FL (O₂ flow rate), Pump

Setting range

Data output

Culture controller

pH (Hydrogen ion concentration) 0.00 - 14.00 ［－]FL (O2 flow rate)
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
FL (O2 flow rate)
pH (Hydrogen ion concentration)

5 - 200 [min ]Power of band heater

8

Name
Model
Control

CO2
AIR

Connection port φ6 one touch tube fitting
Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting
Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting
Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting*All of N₂, O₂, CO₂, and AIR must be dry and clean gasesthat do not contain corrosive components, dust, and oil mist.

O2

CO2
AIR

connection port φ6 one touch tube fitting
Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting
Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting*All of O₂, CO₂, and AIR must be dry and clean gasesthat do not contain corrosive components, dust, and oil mist.

System development and customization for demand specification are also possible.
Please contact our Bioprocess Equipment Division below for more information.
Contact number +81-48-471-9202 e-mail address：bio@satake.co.jp

9Continuous cultur e ( A nim al c ell c u ltu r e, Al ga e b i oma s s , Re ge n e r a t i v e me di c i n e )MRF-RB Reactor

Low-power, low shear action continuous BioReactor equipped with high-efficiency mixing system

Low -shear, high-dispersion mixing sys t e m c om b i n e d w i t h t h e R B Mi xi n g S y s t e mDevelopment, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications
The MRF-RB Reactor is a BioReactor developed as a highperformance mixers with equipped with the low-shear, highefficiency Satake Super-mix® RB Mixing System that has beenused in up to 3,000-ton class biomass reactors. It is a nextgeneration mixing system that utilizes the boundary layer
effect and principles of tornadoes, offering uniform dispersion
performance with superb low shearing and low power
performance. In continuous culture applications with thebasic condition that there is no liquid surface fluctuation, thisproduct is optimal not only for algae cultures, but also animal
cell cultures and regenerative medicine. It is extremely easy toscale up, making it optimal for production consideration during
the research stage for the actual machine. It is also possible tocustomize for single use.

■ MRF-RB

Super-mix®
RB Mixing System

C on t i n u ou s c u l t u r e (An i m a l c e l l c u l t u r e , Al g a e b i o m a s s , R e g e Anea tai vl ec eml lecdui cl ti un ree)
n ri m

O pt i on Simplified controller

RB Mixing System■ RB Mixing system■ MRF-RB Reactor

Item

Specifications

Name

MRF-RB Reactor
MRF-RB-3

MRF-RB-10

Band heater (PID control) with over-temperature protection function (Max. 80 [℃])Power transmission

Magnet drive (non-sealing type)Gas supply type

Shirasu porous glass (SPG) membrane type / sintered metal type (selectable)

Temp. control range

Room temperature + 5 - 20 [℃] (normally set to 37 [℃])

Temp. accuracy±0.3 [℃]（37 [℃]）Configuration

Culture tank

Temp. setting

Touch panel input, data output DC1 - 5 [V]Touch panel input, data output DC0 - 10 [V]Max. 160 [W]

Max. 480 [W]

Power of motor

Max. output 100 [W]

Mixing impeller

RB Mixing System
I.D. 140 x Depth 203 [mm]I.D. 200 x Depth 360 [mm]Culture operation volume

2.4 [L]

7 [L]

Usage condition

10 - 35 [℃]

Outer dimensions

W360×D485×H905 [mm]Power supply

Setting range

Control type

MTA of outer surface
Installation

Outer dimensions/weight
Usage conditions

Sensors

Dimensions

Weight10

5 - 200 [min ]Speed setting
Power of band heater

Display range/accuracy

Data output-1

Rotational speed range

*1

Name
Model
Control

Temp. control device

Function

Flow action (upper)/shear stress (lower)

CFD simulation analysis results■ S-BOX × 10 α II / S-BOX × 10 Simple

Specifications

Item

PerformanceHigh depth,
high circulation formation

The role of impellers in the RB mixing system is not active mixing. The
hint lies in natural flow and rectification action, and tornadoes, which are
powerful enough to lift up houses. We wondered what the rectification
action and tornado forces would create inside the mixing tank. The RB
mixing system consists of an impeller, which generates a swirling flow
inside the mixing tank, and radial blades that efficiently exchanges the
swirling flow (boundary layer effect) toward the center at the bottom of
the mixing tank with a tornado-shaped upward flow, forming a systemthat creates flow patterns that were unthinkable in conventional mixing.
The ”RB” in the name comes from its Radial Blade component. Because
the swirling flow is the main flow, the relative velocity difference near theblade is diminished, creating an extremely low shear action and superb
uniform cell dispersion inside the culture tank, resulting in efficient
mixing for biochemicals, pharmaceuticals, energy generation (water
treatment), and other applications.

■ Mixing method comparison table

JPN Pat. No.3578782
Other patents have been also applied or registered in japan or other countries.

*1■ RB Mixing System

In general, mixing is accomplished by using impellers to fluidize liquid.

This is a simple and inexpensive
control BOX that provides DO
control/ph control using ON/OFF
control. Please select dependingon your purpose.

Model■ Conventional mixing

Approx. 30 [kg]Approx. 34 [kg]AC100 [V], 50/60 [Hz]The performance is the value under room temperature 20 [℃], power supply AC100 [V], 50 [Hz] and no load condition.

Culture controller

S-BOX×10αII

S-BOX×10 Simple

pH, DO (Dissolved oxygen), FL (O₂ flow rate), Pump
pH (Hydrogen ion concentration) 0.00 - 14.00 ［－]DO (Dissolved oxygen)

FL (O2 flow rate)
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
FL (O2 flow rate)
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
FL (O2 flow rate)
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
FL (O2 flow rate)
Rotational speed
Temperature sensor

0.00 - 20.00 [mg/L]

pH (Hydrogen ion concentration)

F.S.

DO (Dissolved oxygen)

0.00 - 20.00 [mL/min]
0.00 - 14.00 ［－]0.00 - 10.00 [mg/L]
0.00 - 20.00 [mL/min]
ON/OFF control
O2 addition/subtraction step control
O2 and N2 ON/OFF control
O2 PI control (slope set method: TIME, %)PI control (slope set method: TIME, %)With data logger
DC0 - 5 [V]Accuracy: ±0.5 [%] F.S.

DC0 - 10 [V]DC1 - 5 [V]Indoor tabletop type

W350×D400×H530 [mm] ・Temperature

5 - 45 [℃]

Humidity

pH, DO (Dissolved oxygen)

Display accuracy: ±0.5 [%]SUS304 (no coating), indoor type, non-waterproof, non-explosion proof specification

pH (Hydrogen ion concentration)
DO (Dissolved oxygen)

Display accuracy: ±0.5 [%]F.S.

0.00 - 20.00 [mg/L]
0.00 - 14.00 [－]0.00 - 10.00 [mg/L]

pH (Hydrogen ion concentration)
ON/OFF control
DO (Dissolved oxygen)
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
Rotational speed
Temperature sensor

With data logger
DC0 - 5 [V]DC0 - 10 [V]DC1 - 5 [V]Accuracy: ±0.5 [%] F.S.

SUS304 (no coating), indoor type, non-waterproof, non-explosion proof specification

Indoor tabletop type

Approx. 15 [kg]20 - 85 [%] RH (No condensation)

0.00 - 14.00 [－]W260×D300×H350 [mm] ・Temperature

Polarographic DO sensor/pH sensor manufactured by Mettler Toledo company

5 - 45 [℃]

Humidity

Approx. 12 [kg]20 - 85 [%] RH (No condensation)

Optical DO sensor manufactured by Automatic System Research Co., Ltd.

(Option: Optical DO sensor manufactured by Automatic System Research Co., Ltd.)+ pH sensor manufactured by Mettler Toledo company

Power supply AC100 [V], 50/60 [Hz], electrical outlet 2 gang (for main control unit and recorder)
Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa],

Power supply AC100[V], 50/60 [Hz], electrical outlet 2 gang (for main control unit and recorder)
Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa],

N2

O2

Utilities

Culture controller

CO2
AIR

Connection port φ6 one touch tube fitting
Flow rate 20 [mL/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting
Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting
Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting*All of N₂, O₂, CO₂, and AIR must be dry and clean gasesthat do not contain corrosive components, dust, and oil mist.

O2

CO2
AIR

connection port φ6 one touch tube fitting
Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting
Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa],
connection port φ6 one touch tube fitting*All of O₂, CO₂, and AIR must be dry and clean gasesthat do not contain corrosive components, dust, and oil mist.

System development and customization for demand specification are also possible.
Please contact our Bioprocess Equipment Division below for more information.
Contact number +81-48-471-9202 e-mail address：bio@satake.co.jp

11Regenerative med ic ine / L o w shear r esis t a n c e c e l l

R e g e n e r a t i v e m e d i c i n e / L o w s hAenairmraels icsetlal nccuel tcuer lel

VMF Reactor

Control of the shearing action and a good mixing action are both established, and an
environment that is best suited for cell culture is created!!

Vert ic al reciprocating motion type mixe r for c e l l c u l t u r e
Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications■ Mixing impeller used
exclusively for vertical
reciprocating motion

VM100

VM200

■ Comparison of shear stress

Dedicated controller

Flat turbine(rotating type）

VMF Reactor(Vertical reciprocating motion type)

VMF Reactor (Vertical reciprocating motion type)■ Culture example

Comparison of cell culture by CHO cells
The VMF reactor provides cells with the optimal physicalenvironment in the culture tank, creating a suitable tankenvironment for cell cultivation compared to rotational
BioReactors, maintaining a high rate of living cells
compared to MRF reactors.
Although this superior effect is weakened for cells thatare resistant to shear damage, this product offers superior
performance for cell culture that relies on shear damage.
This results in increased productivity.

— VMF Reactor（vertical reciprocating motion）— Flat turbine (rotating type)cell culture time [hr]■ S-BOX × 10 α II
Specifications
VMF Reactor

Model
Temp. control device

VMF-05

Linear shaft drive non-sealing typeGas supply type

Liquid surface gas absorption (option: sintered metal type)

Performance

Temp. accuracy±0.3 [℃]（37 [℃]）Max. shaft speed

300 [mm/s]

Temp. setting

Touch panel input, data output DC1 - 5 [V]Power of band heater
Power of motor

Mixing impellers
Culture tankDimensions

Culture operation volume

Usage condition

Power supply

Max. 160 [W]

Max. output 800 [W]

Max. 480 [W]

I.D. 90 x Depth 200 [mm] I.D. 110 x Depth 169 [mm] I.D. 140 x Depth 203 [mm] I.D. 200 x Depth 360 [mm]0.3 [L]

1.2 [L]

2.4 [L]10 - 35 [℃]

7 [L]Approx. 28 [kg]S-BOX×10αII
pH, DO (Dissolved oxygen), FL (O₂ flow rate), Pump

Display range/accuracy

pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
FL (O2 flow rate)
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
FL (O2 flow rate)
pH (Hydrogen ion concentration)

MTA of outer surface
Installation
Outer dimensions/weight
Usage conditions

Approx. 30 [kg]AC100 [V], 50/60 [Hz]The performance is the value under room temperature 20 [℃], power supply AC100 [V], 50 [Hz] and no load condition.
Depending on the Culture operation volume, the only mixing impeller may be only the VM200.

With data logger DC0 - 5 [V]Accuracy: ±0.5 [%] F.S.

(option : Load factor can be outputted.)
DC1 - 5 [V]
SUS304 (no coating), indoor type, non-waterproof, non-explosion proof specification
Indoor tabletop type
W350×D400×H530 [mm]Temperature

Sensors

Utilities

Display accuracy: ±0.5 [%] F.S.

O2 addition/subtraction step control
O2 and N2 ON/OFF control
O2 PI control (slope set method: TIME, %)PI control (slope set method: TIME, %)FL (O2 flow rate)
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
FL (O2 flow rate)
Mixing frequency
Temperature sensor

N2

Approx. 34 [kg]0.00 - 14.00 [－]0.00 - 20.00 [mg/L]
0.00 - 20.00 [mL/min]
0.00 - 14.00 [－]0.00 - 10.00 [mg/L]
0.00 - 20.00 [mL/min]
ON/OFF control

DO (Dissolved oxygen)

Power supply

W360×D485×H905 [mm]Approx. 28 [kg]ModelControl

Data output

VM100+VM200 *2

VM200

Outer dimensions
WeightMax. 105 [W]Specifications
Culture controller

Control type

Touch panel input, data output DC0 - 5 [V]Max. 60 [W]

Item

Name

Setting range

40 [mm]Vertical motion setting

Configuration

VMF-10

Room temperature + 5 - 20 [℃] (normally set to 37 [℃])

Temp. control range
Max. shaft stroke

Function

12

VMF-3

Band heater (PID control) with over-temperature protection function (Max. 80 [℃])Power transmission
*1

*1
*2

VMF-1

10sec

MixingImage source：Mettler-Toledo International Inc.

Item

６sec

Completion

cell viabillity [cells/mL]

This controller enables continuous DO and ph control with
non-contact sensors, also applicable to single use.

Name

４sec

From the CFD simulation analysis results, it is clearly shown that, shear stress is more uniform in VMF reactor using novel vertical motion impeller,
compared to the conventional reactor using the rotating type of flat turbine impeller.
Furthermore, comparison of mixing performance at similar power consumption showed that, complete mixing also can be reached.

O pt i on with non-contact sensor■ VMF Reactor

２sec

Mixed start

A dedicated controller “S-BOX × 10 α Ⅱ ”which can control
DO,pH is provided. Moreover analog singnals can be input todata logger and record them. Through customization, up to

JPN Pat.No.5702924
USA Pat.No. 8,246,242
Other patents have been also applied or registered in japan or other countries.

(Pv=const.)

Flat turbine (rotating type）

CFD Simulation Analysis Result

The VMF Reactor is a vertical reciprocating motion typenext-generation reciprocating mixer for cell culture thatcombines together excellent blending performance andgentle mixing. Unlike the general rotating type, severe
shear stress control is possible. Moreover, a “completely
enclosed structure” that does not require a complex
rotating-axis seal mechanism is implemented. There is no
threat of contamination or leakage, and it has a high levelof sterility and cleanliness.

eight machines in series are supported.

■ Comparison of mixing Performance

O2

CO2
AIR

5 - 45 [℃]

Humidity

・ Approx. 15 [kg]20 - 85 [%] RH (No condensation)

Polarographic DO sensor/pH sensor manufactured by Mettler Toledo company(Option: Optical DO sensor manufactured by Automatic System Research Co., Ltd. )AC100 [V], 50/60 [Hz], electrical outlet 2 gang (for main control unit and recorder)

Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
Flow rate 20 [mL/min] or lower, supply pressure 0.2 [Mpa] connection port φ6 one touch tube fitting

Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting

Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting*All of N2, O₂, CO₂, and AIR must be dry and clean gases that do not contain corrosive components, dust, and oil mist.

System development and customization for demand specification are also possible.
Please contact our Bioprocess Equipment Division below for more information.
Contact number +81-48-471-9202 e-mail address：bio@satake.co.jp

13Regenerative med ic ine / L o w shear r esi s t a n c e c e l l

R e g e n e r a t i v e m e d i c i n e / L o w s hAenairmraels icsetlal nccuel tcuer lel

C ol l a b o r a t i o n o f VM F Rea ct o r/SPG m e m b r an e s p ar ge r
Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications
The VerSus Reactor is a new and innovative BioReactor
for animal cell culture in which the technology of “SPG
membrane sparger*” by which micro-bubbles withextremely high homogeneity can be generated is fusedwith a VMF Reactor. Through an efficient oxygen supply
by the SPG membrane sparger, oxygen can be supplied
efficiently without putting stress on the animal cells in theculture tank. Also, due to a decline in the DO air flow rate,a foam layer can be prevented. A decicated controller “S-BOX■ SPG membrane sparger

The micro-bubbles can be homogeneously
dispersed inside the tank.

× 10 α Ⅱ ”which can control DO,pH is provided. Moreover
analog singnals can be input to data logger and record them.

We promise further improvement in production efficiency with the “VerSus Reactor” which
is a collaboration of the “VMF Reactor” that controls the physical action in the cell culturetank and “SPG membrane sparger”, which is an excellent micro bubble technology.
■ Culture example

Comparison of cell viability by CHO cells

cell viability [cells/mL]

VerSus Reactor®

up/down motion—— Without SPG membrane sparger
With SPG membrane sparger*SPG membrane sparger has been developed jointly by MIYAZAKI

The growth curve of CHO cells based on the presenceof SPG membrane sparger is shown by using the VMF
reactor. It is understood that the culture results improves
significantly by using the SPG membrane sparger. Due tomicro-bubbles having extremely high homogeneity, thehigh gas absorption performance significantly reduces the
DO air flow rate, which is the cause of formation of a foamlayer, without disturbing the weak flow. VerSus reactor hasa significantly improved total performance.

PREFECTURE INDUSTRIAL TECHNOLOGY CENTER and JGC Corporation.

O pt i oncell culture time [hr]Dedicated controller
with non-contact sensor

Comparison of production of antibodies by CHO cells

This controller enables continuous DO and ph control with
non-contact sensors, also applicable to single use.
Conc. of Antibody [g/L]
JPN Pat.No.6534665
USA Pat.No. 10,799,841
Other patents have been also applied or registered in japan or other countries.

Image source：Mettler-Toledo International Inc.

Item

Specifications

Name
Model
Temp. control device

VerSus Reactor
VSR-05

VSR-1

VSR-3

Band heater (PID control) with over-temperature protection function (Max. 80 [℃])
Linear shaft drive non-sealing typeGas supply type

Shirasu porous glass (SPG) membrane type (additional option : sintered metal type)
Room temperature + 5 - 20 [℃] (normally set to 37 [℃])

Temp. control range

40 [mm]Vertical motion setting

Configuration

Power of band heater
Power of motor

Mixing impellers
Culture tankDimensions

Culture operation volume

Usage condition

Power supply

Specifications
Culture controller

Model

S-BOX×10αII

Control

pH, DO (Dissolved oxygen), FL (O₂ flow rate), Pump

Display range/accuracy
Setting range

Touch panel input, data output DC0 - 5 [V]
60 [W]

105 [W]

160 [W]

Max. power 800 [W]

480 [W]

VM100+VM200 *2

VM200

I.D. 90 x Depth 200 [mm] I.D. 110 x Depth 169 [mm] I.D. 140 x Depth 203 [mm] I.D. 200 x Depth 360 [mm]0.3 [L]

1.2 [L]

2.4 [L]10 - 35 [℃]

7 [L]Approx. 28 [kg]Approx. 30 [kg]AC100 [V], 50/60 [Hz]*1

The performance is the value under room temperature 20 [℃], power supply AC100 [V], 50 [Hz] and no load condition.

*2

Depending on the Culture operation volume, the only mixing impeller may be only the VM200.

Data output
MTA of outer surface
Installation
Outer dimensions/weight
Usage conditions

With data logger DC0 - 5 [V]Accuracy: ±0.5 [%] F.S.

(option : Load factor can be outputted.)
DC1 - 5 [V]
SUS304 (no coating), indoor type, non-waterproof, non-explosion proof specification
Indoor tabletop type
W350×D400×H530 [mm]Temperature

N2

Utilities

Display accuracy: ±0.5 [%] F.S.

O2 addition/subtraction step control
O2 and N2 ON/OFF control
O2 PI control (slope set method: TIME, %)PI control (slope set method: TIME, %)FL (O2 flow rate)
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
FL (O2 flow rate)
Mixing frequency
Temperature sensor

Power supply

Approx. 34 [kg]0.00 - 14.00 [－]0.00 - 20.00 [mg/L]
0.00 - 20.00 [mL/min]
0.00 - 14.00 [－]0.00 - 10.00 [mg/L]
0.00 - 20.00 [mL/min]
ON/OFF control

DO (Dissolved oxygen)

Sensors

W360×D485×H905 [mm]Approx. 28 [kg]pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
FL (O2 flow rate)
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
FL (O2 flow rate)
pH (Hydrogen ion concentration)

Control type

300 [mm/s]

Outer dimensions
WeightItem

Touch panel input, data output DC1 - 5 [V]Temp. setting

Function※ The result of joint research with JGC Corporation

Name±0.3 [℃] (37 [℃])

Temp. accuracy

Max. shaft stroke
Max. shaft speed14

VSR-10Power transmission

Performance

Cultivation time [day]■ S-BOX × 10 α II

■ VerSus Reactor*1

The result* of scale-up from the control quantity of 8 l
to 150 l by using CHO cells having high dependabilityon the shear stress is shown below. Here, the actuallycommercially-available production of antibodies isundertaken. As a result, in the 150-l scale-up condition,either almost the same or more volume of production of
antibodies than the control quantity of 8 l was obtained.
This scale-up is performed by the numerical fluid
calculation at a constant shear factor, and its advantage inthe combined use of computational fluid dynamics and cell
culture has been proved.

up/down motion— Volume：8L— Volume：150L

O2

CO2
AIR

5 - 45 [℃]

Humidity

・ Approx. 15 [kg]20 - 85 [%] RH (No condensation)

Polarographic DO sensor/pH sensor manufactured by Mettler Toledo company(Option: Optical DO sensor manufactured by Automatic System Research Co., Ltd. )AC100 [V], 50/60 [Hz], electrical outlet 2 gang (for main control unit and recorder)

Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
Flow rate 20 [mL/min] or lower, supply pressure 0.2 [Mpa] connection port φ6 one touch tube fitting

Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting

Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting*All of N2, O₂, CO₂, and AIR must be dry and clean gases that do not contain corrosive components, dust, and oil mist.

System development and customization for demand specification are also possible.
Please contact our Bioprocess Equipment Division below for more information.
Contact number +81-48-471-9202 e-mail address：bio@satake.co.jp

15Regener a ti v e med i c i ne / i PS Dru g d i sc o v e ry / Low s h e a r r e s i s t a n c e c e l l
R e g e n e r a t i v e m e d i c i n e / i P S D r u g d i s c o v e r y / L o w s hAenairmraels icsetlal nccuel tcuer lel

VMF-WSUB Reactor/TCS Controller
Equipped with weight management perfusion system for commercial regenerative medicine production
Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications
When using iPS cells in 3D floating
undifferentiated culture for regenerative
medicine, efficient culture medium replacement
is important. By stopping the mixing BioReactor
for culture medium replacement, cells settle
to the bottom of the culture tank having
the ability of arbitrarily sticking together on
the spot. This process results in uneven cellaggregation (sphere diameter), sometimes
adversely affecting induced differentiation
efficiency. When induced differentiation is thegoal, single cells are discharged along withthe culture medium, making it necessary tomodify the inside of the culture tank so thatthey are not affixed to the surface. To satisfy
these requirements, clogs must be avoided andculture medium discharge must be stabilized
over a long period of time. A control system is
an important part of maintaining continuous,
precise culture medium replacement. The VMFWSUB / TCS system has achieved these goals
and features specifications that are capable ofactual production.

Long-term continuous medium replacement andautomated medium replacement are controllable!

Specifications for commercial
production machines

A perfusion system that makes long-term, continuous culturing possible!
Long-term, clog-free operation from undifferentiated cultures to differentiation!

Mixing BioReactors experience frequent weightfluctuations, and this production system makes it
possible to accurately gauge only the target weightinside the culture tank as well as culture medium
replacement and perfusion control.

Item

Specifications

Name

VMF-W Reactor

Model
Temp. control device
Power transmission

Display range/accuracy

Gas supply type*1

Performance

Culture tank

DO (Dissolved oxygen)

Control typeFL (O2 flow rate)

Room temperature + 5 - 20 [℃] (normally set to 37 [℃])Max. shaft stroke

40 [mm]pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
FL (O2 flow rate)
Mixing frequency
Temperature sensor
In-vessel weight
Supply/discharge balance weight
Supply/discharge pump speed

Temp. accuracy±0.3 [℃] (37 [℃])Max. shaft speed

300 [mm/s]

Mixing impellers

VM200

Dimensions

Culture operation volume

Outer dimensions
WeightPower supply

Data output

Touch panel input, data output DC0 - 5 [V]

60 [W] (Max. 60 [℃])Power of motor

Fed-batch control (2)Touch panel input, data output DC1 - 5 [V]Power of band heater

Usage condition

Max. power 800 [W]

I.D. 94/87 x Depth 110 [mm]0.25 - 0.3 [L]Approx. 28 [kg]

160 [W] (Max. 60 [℃])
VM100+VM200

I.D. 159/138 x Depth 202 [mm]10 - 35 [℃]

W360×D485×H905 [mm]AC100 [V], 50/60 [Hz]*1 The performance is the value under room temperature 20 [℃], power supply AC100 [V], 50 [Hz] and no load condition.

16

pH, DO (Dissolved oxygen), FL (O₂ flow rate), Pump ON/OFF
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
FL (O2 flow rate)
In-vessel weight
Supply balance weight W1
Discharge balance weight W2
Supply pump speed P1
Discharge pump speed P2
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
FL (O2 flow rate)
In-vessel weight
Supply balance weight W1
Discharge balance weight W2
Supply pump speed P1
Discharge pump speed P2
pH (Hydrogen ion concentration)

Temp. control range

Vertical motion setting

Configuration

Liquid surface gas absorption (option : sintered metal type)

Culture controller
S-BOX×TCS

Fed-batch control (1)Temp. setting

Function

VMF-3W

Band heater (PID control) with over-temperature protection function (Max. 60 [℃])
Linear shaft drive non-sealing typeSpecifications

Item

Name
Model
Control

Setting range

VMF-05W

1.8 - 2.5 [L]

MTA of outer surface
Installation
Outer dimensions/weight
Usage conditions

Display accuracy: ±0.5 [%] F.S.

0.0 - 200.0 [min-1]
0.0 - 200.0 [min-1]
0.00 - 14.00 [－]0.00 - 10.00 [mg/L]
0.00 - 20.00 [mL/min]
0 - 6118 [g]-3200.00 - 3200.00 [g]-3200.00 - 3200.00 [g]

0.0 - 200.0 [min-1]
0.0 - 200.0 [min-1]
ON/OFF control
O2 addition/subtraction step control
O2 and N2 ON/OFF control
O2 PI control (slope set method: TIME, %)PI control (slope set method: TIME, %)Intermittent culture medium replacement control by in-vessel weight and roller pumpContinuous culture medium replacement control by in-vessel weight and roller pump
Intermittent culture medium replacement control by culture medium supply/discharge balance weight and roller pumpContinuous culture medium replacement control by culture medium supply/discharge balance weight and roller pumpWith data logger
DC0 - 5 [V](option : Load factor can be outputted.)

Accuracy: ±0.5 [%] F.S.

DC1 - 5 [V]Digital input type
SUS304 (no coating), indoor type, non-waterproof, non-explosion proof specification
Indoor tabletop type

Temperature

5 - 45 [℃]

Approx. 26 [kg]Humidity

20 - 85［%］ RH (No condensation)

Polarographic DO sensor/pH sensor manufactured by Mettler Toledo company

Sensors(Option: Optical DO sensor manufactured by Automatic System Research Co., Ltd.)
N2

Utilities

0.00 - 14.00 [－]0.00 - 20.00 [mg/L]
0.00 - 20.00 [mL/min]
0 - 6118 [g]-3200.00 - 3200.00 [g]-3200.00 - 3200.00 [g]W350×D400×H538 [mm] ・Power supply

Approx. 30 [kg]This culture medium replacement holder and membrane set
keeps the tank free from clogging for long periods of timeand offers superb practical separation of cells and culture
medium inside the tank.
There are several different types of membrane section
depending on your purpose.

■ S-BOX × TCS

JPN Pat.No.5702924
USA Pat.No. 8,246,242
Other patents have been also applied or registered in japan or other countries.

■ VMF-W Reactor■ Culture medium replacement holder andmembrane set MED-CH■ Load cell for culture tank weight measurement

O2
CO2
AIR

AC100 [V], 50/60 [Hz], electrical outlet 2 gang (for main control unit and recorder)
Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
Flow rate 20/50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting
Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting*All of N2, O₂, CO₂, and AIR must be dry and clean gases that do not contain corrosive components, dust, and oil mist.

System development and customization for demand specification are also possible.
Please contact our Bioprocess Equipment Division below for more information.
Contact number +81-48-471-9202 e-mail address：bio@satake.co.jp

17Regenerative medic ine / iPS Dr u g d isc o v e r y / L ow s h e a r r e s i s t a n c e c e l l

Regenerative medicine / iPS Drug discovery / Low shear resistance cell

Dedicated data logger / software

HiD ４×４

3 D f l oa t ing iPS c e l l d i ffe re n t i a t i on in d u ct io n B io R e a ct o r r
Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications
The HiD 4 × 4 Reactor is the world’s first“Single-use mixer for 3D floating cell cultures”
used exclusively for iPS cell differentiationinduction. A BioReactor that achieves ahigh-volume production of uniform andhomogeneous iPS cells is indispensable not
only in regenerative medicine, but also in the“Use of human iPS cells in drug development”
that aims at industrialization and commercial
production. By promoting joint researchand development with excellent research
institutes or companies in Japan, we havesucceeded in commercialization an iPS celldifferentiation induction BioReactor for the
first time in the world. 4, 8, 12, and 16 (or evenmore) such BioReactors can be controlled in aconsolidated manner, and these are also best
suited for screening. Moreover, we have also
taken into consideration the adaptation to ES
cells while performing development. With thehelp of the dedicated controller “S-BOX × 02”,changes in various parameters including PI
control, and production control in accordancewith the purpose can be performed.

Using a dedicated data logger connected to a computer via
Ethernet cable and Satake Chemical proprietary software, you
can set an interval at which to acquire data on changes overtime such as DO, pH, temperature, O2 flow rate, frequency,
and number of revolutions during culturing, which can thenbe saved in
CSV format.

Specifications

Name

HiD4×4Model

HiD4-4

Temp. control device

Hot plate + chiller / heating & cooling type (PID control) with over-temperature protection function

Power transmission

Linear shaft drive non-sealing type*1

Performance

Function

Configuration

Culture tank

Culture operation volume0.25 - 0.3 [L]Usage condition

18

Material

Polycarbonate

base

Outer dimensions

W57.7×D103.5×H135 [mm]GM90MB

WeightApprox. 0.15 [kg]S-BOX×02

Display range/accuracy

pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
pH (Hydrogen ion concentration)
DO (Dissolved oxygen)
pH (Hydrogen ion concentration)

MTA of outer surface
Installation
Outer dimensions/weight
Usage conditions

0.00 - 14.00 [－]0.00 - 20.00 [mg/L]
0.00 - 14.00 [－]0.00 - 20.00 [mg/L]
CO2 ON/OFF control
O2 ON/OFF control
With data logger DC0 - 5 [V]Display accuracy: ±0.5 [%] F.S.

(option : Mixing frequencyand Load factor can

Accuracy: ±0.5 [%] F.S.
be outputted.)
Temperature sensor
With data logger DC1 - 5 [V]
SUS304 (no coating) , indoor type, non-waterproof, non-explosion proof specification

DO (Dissolved oxygen)

Indoor stand-alone type

Temperature

W600×D500×H914 [mm] *Does not include protrusions approx. 70 [kg]5 - 45 [℃]

Humidity

20 - 85 [%] RH (No condensation)

Polarographic DO sensor/pH sensor manufactured by Mettler Toledo company(Option: Optical DO sensor manufactured by Automatic System Research Co., Ltd.)Power supply AC100 [V], 50/60 [Hz], electrical outlet 2 gang (for main control unit, and laptop)

10 - 35[℃]
Main unit (HiD4×4)

W680×D480×H914 [mm]Chiller (SCA-32)

W205×D405×H545 [mm]WeightMain unit (HiD4×4)

Approx. 70 [kg]Chiller (SCA-32)

Approx. 28 [kg]

*1 The performance is the value under room temperature 20 [℃], power supply AC100 [V], 50 [Hz] and no load condition.

Approx. 0.55 [kg]ModulepH, DO (Dissolved oxygen)

Sensors

AC100 [V], 50/60 [Hz]W88×D126.7×H135 [mm]WeightControl

Data output

Outer dimensions
Power supply

Outer dimensions

Culture controller

Touch panel input, data output DC1 - 5 [V]I.D. 94/87 x Depth 110 [mm]AC 100 - 240 [V]Name

Temp. setting

Dimensions

Polycarbonate

Rated voltage

Model

150 [mm/s]

VM200

2.8 [W] or lessMaterial

Specifications

Max. shaft speed

Mixing impeller

Power consumption

Item

Control typeMax. output 800 [W]

From GM90PS power supply module

The bottles have already undergone EOG sterilization.
We have prepared certification for each lot.
An elution certificate can be provided (at an additionalcharge) upon request for production specifications.

40 [mm]Power of motor

Power supply

Sterilized standard bottles

Max. shaft stroke

Cooling type, 450 [W], HFC R-404A

Approx. 0.25 [kg]GM10Materials conforming to USP Class VI (United States Pharmacopoeia Class 6)
are used. All these materials have been developed jointly by Japan’s material
manufacturers. Since these are Made in Japan, we promise to provide a stablesupply at all times even in small lots. There is no need to have a lot of Inventory
due to the supply risk caused by overseas products.

±0.3 [℃] (37 [℃])

Chiller

W45.1×D107.1×H111 [mm]WeightVarious materials used in impellers, bottles, and bags

Temp. accuracy

235 [W]×4

Outer dimensions

moduleThis product features a 0.5 L single-use bottle as a standard, making it perfect for upscaling and commercial or industrial
production. It is designed not only for safety, but with a focus on usability for more efficient culturing.

Setting range

Power of hot plate

Data gathering

Single-use bottle 0.5 L

Room temperature + 5 - 20 [℃] (normally set to 37 [℃])

Touch panel input, (option : mixing frequency data output DC0 - 5 [V] )Polycarbonate

moduleTemp. control range

Vertical motion settingSpecifications

Material

GM90PS■ S-BOX × 02
Item

Item

Power supply

JPN Pat. No.5702924
USA Pat.No. 8,246,242
Other patents have been also applied or registered in japan or other countries.

■ HiD 4 × 4■ Data logger

Utilities

N2

Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting

O2

Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting

CO2

Flow rate 50 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting

AIR

Flow rate 150 [mL/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting*All of N₂, O₂, CO₂, and AIR must be dry and clean gases that do not contain corrosive components, dust, and oil mist.

System development and customization for demand specification are also possible.
Please contact our Bioprocess Equipment Division below for more information.
Contact number +81-48-471-9202 e-mail address：bio@satake.co.jp

19

Single-use series

Single-use series

Single-use BioReactor

Each sensor

VMF-50L/200L SUB

Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications

VMF-50L/200L SUB is Single-use Bioreactor for commercial
production, and the lineup consists of models from 50 to
200L (development planned for up to 1,000L).Single-ues
Bioreactor from 0.5 to 10L is tabletop type. On the other
hand, VMF-50L/200L SUB applied for 50L and more is selfstanding type. This is the largest model in the standard
VMF reactor series and a commercial production device
that is perfectly scalable, offering an unprecedented
starting size of 0.5L.

Single-Use Bioreactor

JPN Pat. No.5702924
USA Pat.No.8,246,242
Other patents have been also applied or registered in japan or other countries.

Culture tanksheath. It enables accurate temperature readings ofproduction equipment without injecting water into thesheath.

● A completely hermetically sealed construction
ensures a contamination-free environment.

ItemSpecifications

Name

VMF Reactor

Model

VMF-50L SUB *1
Linear shaft drive non-sealing typeGas supply type

Shirasu porous glass (SPG) membrane type / sintered metal type (selectable)

Temp. control range

Room temperature + 5 - 20 [℃] (normally set to 37 [℃])

Temp. accuracy±0.3 [℃] (37 [℃])Max. shaft stroke

100 [mm]Max. shaft speed

800 [mm/s]

Temp. setting

S-BOX×200 Touch panel input

Vertical motion setting

S-BOX×200 Touch panel input

Power of rubber heater

1.5 [kW]Power of motor

Max. output 800 [W]

Mixing impellers

VM100+VM200

Dimensions

I.D. 369 x Depth 650 [mm]Culture operation volume

40 - 45 [L]Usage condition

10 - 35 [℃]

Outer dimensions

W780×D800×H2000 [mm]WeightMain unit approx. 135 [kg]Power supply

Power is supplied from controller S-BOX × 200*1 For VMF-200L SUB, please contact us for further information.
*2 The performance is the value under room temperature 20 [℃], power supply AC200 [V], 50 [Hz] and no load condition.

20

temperature sensor is installed inside a silicone tube

The S-BOX ×200 controller is included as a standard,offering full control over DO, pH, temperature, in-bagpressure, etc., and is equipped with four embedded
pumps in a series, a dedicated internal digital datalogger, Satake data logger software, and a BioReactor
data collection system, etc. It also supports computerized
system validation, and documents are provided for
qualification confirmation during the design stage,
during installation, and during operation. We also provide
support for clients who are not accustomed to creatinguser requested specifications. We guarantee just-in-time
supply in small lots of locally-manufactured single-use
bags for sterilization validation.
We can meet various demands required for commercial
production, so please feel free to contact us.

Rubber heater (PID control) with over-temperature protection function

Configuration

with a sterile AseptiQuik Connector from CPC. The■ Bag for Single-use

Power transmission

Functiona Thermo Fisher Scientific bioreactor probe assembly※ Regarding to the tabletop type, please refer to VMF Reactor P12-13.

Temp. control device

Performancethe photo. The DO and pH sensors are attached using

CFD simulation analysis results

JNP Patent No.6313635● S ince this is a 100% local product, we canalways provide a stable supply even in smalllots.
● S i n c e s t e r i l i z a t i o n h a s a l r e a d y b e e nperformed,you need not perform washing andsterilization at the time of use.
● T he bag, impeller, shaft, and tube, etc. use
materials conforming to USP Class VI(United States Pharmacopoeia Class 6).

Model● T he DO/pH sensor is attached after highpressure steam sterilizer in an autoclave.

Consideration of commercial production scale-up● An optional single-use pressure sensor is alsoavailable.

■ S-BOX × 200
Item
Name
Model

Display range/accuracy

S-BOX × 200

pH, DO (Dissolved oxygen), FL (O₂ flow rate), TH (Temperature),
Shaft motion, Medium weight, Pump ON/OFF
TH (Temperature)

0.0 - 150.0 [℃]

DO (Dissolved oxygen)

0.0 - 100.0 [％]Linear shaft (mixing)

Stroke 0-100 [mm], Speed setting 0-800 [mm/s]

FL (O₂ flow rate)

0.00 - 5.00 [L/min]

0.00 - 14.00 [－]Electronic balance (for culture medium supply)

0.1 [g] - 21 [kg]TH (Temperature)

0.0 - 60.0 [℃]

DO (Dissolved oxygen)

0.0 - 100.0 [％]Linear shaft (mixing)

Stroke 0-100 [mm], Speed setting 0-800 [mm/s]

FL (O2 flow rate)

0.50 - 5.00 [L/min]

0.00 - 14.00 [－]Electronic balance (for culture medium supply)

0.1 [g] - 21 [ kg]TH (Temperature)

PI control (slope set method: TIME, %)Linear shaft (mixing)

In-place control by shaft driver
ON/OFF control of CO2 gas supply and alkaline water pump (selectable)

pH (Hydrogen ion concentration)
DO (Dissolved oxygen)

PI control (slope set method: TIME, %) Using mass flow controller

FL (O2 flow rate)

PI control (slope set method: TIME, %)ON/OFF control with FEED pump

FEED

Data output

MTA of outer surface

Culture medium supply/discharge

SUS304 (no coating) , indoor type, non-waterproof, non-explosion proof specification
Indoor stand-alone type
Outer dimensions W550×D550×H1000 [mm] *Does not include protrusions approx. 90 [kg]Outer dimensions/weight
Temperature

5 - 45 [℃]

Humidity

20 - 85 [%] RH (No condensation)

Polarographic DO sensor/pH sensor manufactured by Mettler Toledo company

Sensors(Option: Optical DO sensor manufactured by Automatic System Research Co., Ltd.)Power supply

Utilities

Supply and discharge by electronic balances, culture medium supply, and discharge pumps

pH (hydrogen ion concentration), DO (dissolved oxygen), FL (O2 flow rate), temperature, (option: mixing frequency ,Load factor by data logger 10ch)

Installation

Usage conditions

Shear stressSpecifications

pH (Hydrogen ion concentration)

Control type

Turbulent energy

Culture controller

pH (Hydrogen ion concentration)

Setting range

Flow velocity
distribution

and utilization of numerical fluid calculation

Control■ VMF-50L/200L SUB*2Each sensor is attached to the culture tank as shown in

O2

CO2

1 [φ], AC200 [V], 30 [A] (2P + E, electrical outlet for 30 A hook-type plug) 1 gang
Flow rate 5 [L/min] or lower, supply pressure 0.2 [MPa], connection port φ6 one touch tube fitting

AIR*All of O₂, CO₂, and AIR must be dry and clean gases that do not contain corrosive components, dust, and oil mist.

System development and customization for demand specification are also possible.
Please contact our Bioprocess Equipment Division below for more information.
Contact number +81-48-471-9202 e-mail address：bio@satake.co.jp

21

Single -use

Single-use

Single-use Bottles/Single-use Bags

JPN Pat. No.6313635

In biomedical products, regenerative medicine, and other such areas, single-use products are preferable from the perspectivesof increased productivity and prevention of contamination. At SATAKE, we provide single-use products with total capacities
ranging from 0.5L to 200L (with plans to develop products of up to 1000L in capacity) to meet a wide range of needs. Our
products achieve cell culture in a scalable manner from the laboratory to actual production.

Multipurpose Bag
This bag can be used for various purposes such as supply, discharge, and reagent use during continuous culture medium
replacement. It can also be customized, so please feel free to inquire. γ-ray sterilization is employed to make this bag suitablefor use with pharmaceuticals.

Single-use Bottles
fixings

Dish case for cultures

Bottles/Bags with Single-use Sensors
For DO/pH sensor
fixings (for reuse) included.

VMH-500
1-Stage Impeller
Operation volume: 0.25 - 0.3 L(total capacity 0.5 L)

VMH-1000
1-Stage Impeller
Operation volume: 0.8 - 1.2 L(total capacity 3 L)

VMH-3000
2-Stage Impeller
Operation volume: 1.8 - 2.5 L(total capacity 3 L)

*The 0.5L single-use bottle is compatible with the "VMF Reactor VMF-05 and HiD4 × 4"
*The 3L single-use bottle is compatible with the" VMF Reactor VMF-3" and also with 2 to 8 optional devices connected .

Single-use sensors (DO, pH) made by Mettler Toledo - well-known for culture-related products - are pre-attached to the bottle/
bag. These can be used without autoclave sterilization, enabling preparation time to be shortened. They are also perfect forcontamination prevention.

Fitted as standard with a single-use DO sensor and pH sensor

Single-use Bags (3D)InSUS_607

VMB-10Operation volume: 8 - 9 L(Total capacity 10 L)

VMB-50Operation volume: 40 - 45 L
(Total capacity 50 L)

InSUS_310

VMB-200
Operation volume: 160~180 L(Total capacity 200 L)*The single-use bag is compatible with the "VMF Reactor VMF-10 and VMF Reactor VMF-50L / 200L SUB".

Single-use Bags (2D)2D Bags for Seed Cell Cultures in the Production of Drug Substances for Biopharmaceuticals
Image source：Mettler-Toledo International Inc.

Various materials used in impellers,bottles, and bags
Materials conforming to USP Class VI (United States Pharmacopoeia Class 6) are used. All these materials have been developed
jointly by Japan’s material manufacturers. Since these are Made in Japan, we promise to provide a stable supply at all times.
SCB-10Total capacity 10L

SCB-50Total capacity 50L■ Single-use Bottles/Single-use Bags
Item
Name
Model
Culture operation volume
Dimensions
Ports *1

Single-use Bags (3D)Single-use Bags (2D)

VMH-500

VMH-1000/3000

VMB-10

VMB-50

VMB-200

SCB-100.25 - 0.3 [L]

0.8 - 1.2/1.8 - 2.5 [L]

8 - 9 [L]40 - 45 [L]

160 - 180 [L]

10 [L]

50 [L]

I.D. 94 (87) [mm]I.D. 159 (138) [mm]I.D. 206 [mm]I.D. 369 [mm]I.D. 590 [mm]560×330 [mm]740×705 [mm]Depth 110 [mm]Depth 202 [mm]Gas inlet/outlet, sampling, temperature sensor, DO

Depth 360 [mm]Depth 650 [mm]Depth 1015 [mm]Culture medium inlet/outlet, sampling, substrate inlet, cellsensor, pH sensor, culture medium replacement,

inlet, air inlet/outlet, O2 inlet, temperature sensor, DO sensor,

inoculation port

*1 The port equipped with the bag can be customized by consultation.
*2 Spare port only on SCB-50

22

Specifications

Single-use Bottles

Sterilized standard bottles and bags

pH sensor

SCB-50(Outer dimensions) (Outer dimensions)
Gas inlet/outlet, harvest, sampling, cell
inlet, culture medium inlet, spare*2

We prepare hard bottles and bags in the range of 0.5 to 200L. Since EOG sterilization for hard bottles, γ sterilization for bags
has already been performed, you can start using these products as soon as you receive them. We have prepared certification foreach lot.
Various testing has been conducted and conformity confirmed on each material used so you can use the products immediately
without worry.

Proposal of application development and customization
The single-use bag product lineup has a maximum capacity of 200 L, but we can also support bags of 500 to 1000 L capacity
according to the application.If you have a request for customized products that match the equipment, please contact us.

23

Culture medium ex c hang e

Culture medium exchange

CSS II continuous culture and perfusion system
connectable to Satake BioReactor
Development, Customization, and Single-use support of Exclusive Reactor in Accordance with Requirements Specifications
Culture medium replacement inside
the culture tank utilizes the electronicbalance and feed pump attached tothe controller (CSS II) to supply culture
medium at the set time and weight andsimultaneously discharge the culture
medium.
The feed pump automatically calculates
the optimal number of revolutions fromthe set time, pump flow rate value (selectthe tube diameter) and current weightvalue of the electronic balance, andoperation continues until the specified
time. Additionally, the balance weightis monitored every 0.1 seconds duringcontrol operations, and the numberof pump revolutions automaticallychanges. Control automatically stops
once the specified time is reached. The
standard included pump is generally
used at medicine manufacturing sites
and in culture laboratories: WatsonMarlow 120U/DV analogue control
variable speed pump, and electronicbalance: A&D GX3002A included as astandard.

■ Display panel
Control is started byentering the set valueon the touch panelon the front of thecontroller and pressing
the activation button.

Various bioreactor

Accessories
Electronic balance・pumpController：CSS Ⅱ

Patent pending■ CSS Ⅱ

Item

Specifications

Name
Model

Double Medium Replacement Controller
CSSⅡ

Supply balance weight W1

Display range

Discharge balance weight W2

0.01 - 3200.00 [g]Supply balance weight W3*The specification can be customized in accordance with customer's requirement.

Introducing an example system
The continuous culture and perfusion system CSS II device flow and system flow are shown in the figure. Connecting the supply
and discharge pump and electronic balance as shown in the figure enables automatic culture medium replacement.
Additionally, a single CSS II can be used for culture medium replacement in two culture tanks.

■ Device flow
Discharge
Medium receiverpot

Medium pot

Dischargebalance

Setting range

Supply pump P3 supply volume

Control type
Record Data

Culture medium discharge

MTA of outer surface
Installation

1 [minute] ~Electronic balance/pump
Utilities

24

9999 [hours] 99 [minutes]

Continuous culture medium supply control by weight and fluid feed pump

排出
Discharge

Supply供給

Medium receiver
pot 1Medium pot 1

Discharge
排出用天秤balance
W4
W2Discharge
排出ポンプ
pump
P4
P2

排出
Discharge

Culture
tank
培養槽
LINE2
LINE1

Supply
供給ポンプ
pump
P3
P1

CSSⅡ Controller

Supply
供給用天秤
balance
W3
W1

供給
SupplyMedium receiver
pot 2

Medium pot 2

W1, W2, W3, W4
P1, P2, P3, P4

SUS304 (no paint), Indoor type, non-waterproof, non-explosion proof specification
Tabletop

Outer dimensions/weight
Usage conditions

Supplybalance*The specification can be customized in accordance with customer's requirement.

Balance weight
Pump discharge volume

Supplypump

Display accuracy: ±0.5 [%] F.S.

Culture medium supply / discharge time setting
Culture medium supply

Culture tank

0.0 - 3200.00 [g]Discharge pump P2 discharge volume
Discharge pump P4 discharge volume

Dischargepump■ System flow

Discharge balance weight W4
Supply pump P1 supply volume

SupplyW260×D400×H225 [mm] ・Approx. 10[kg]Temperature

5 - 45 [℃]

Humidity

20 - 85 [%] RH (No condensation)

Electronic balance: A&D Company GX3002A x 2 (Option: GX-22001M)
Pump: Watson-Marlow 120U x 2Power supply

AC100 [V], 50/60 [Hz] electrical outlet 9 gang
(Main control unit, electronic balance x 4, fluid feed pump x 4)

Discharge
排出用天秤balance
W2
W4Discharge
排出ポンプ
pump
P2
P4

Culture tank
培養槽 LINE1
LINE2

Supply
供給ポンプ
pump
P1
P3

Supply
供給用天秤
balance
W1
W3※ The supply and discharge of the system flow chart can be controlled up to 2 systems.
We also provide pump systems and perfusion systems tailored to customer needs.
Please feel free to contact us regarding specialized designs for actual production, such as a single balance/pump configuration, and liquidsurface control to prevent adhesion during culturing and production while conducting perfusion.

25

Culture medium ex c hang e system o p tion

Culture medium exchange system option

Low-temperature Incubator S L I - 4 0 9 0This incubator has an HEPA filter
making it suitable for CPC use

U s e w i th S ATA KE pe rfusion syste ms
Culture medium temperature meets temperature demands for 3 - 10℃
This incubator keeps the culture medium stable
at a low temperature when used with TCS andCSSII perfusion systems.There are two products inthe lineup for use not only in labs but also in cellprocessing centers (CPC).
With an assumed culture medium temperature
display function, this incubator enables moreaccurate temperature management than others.
A culture medium storage vessel smaller thanMultipurpose Bag for 10L can be accommodated.

Laboratory Incubator※ Regarding to Multipurpose Bag, Please refer to page 23.
※ A culture medium storage Bottle up to 2L vessel can beaccommodated.

・A ble to adjust culture medium temperature
to any temperature between 3 - 10℃ ± 1.0℃
(ambient temperature 19℃ )・Equipped with frost removal function (once every
8 hours approx.)・Equipped with automatic condensate evaporator
function・CPC model equipped with low pressure loss HEPA
filter・C PC model equipped with assumed culture
medium temperature analog output function・Equipped with culture medium supply tube holder

SLI-4090-B■ SLI-4090-B

Specifications

Temp. control

SLI-4090-C

Environment temp. range+18 - 22 [℃] *1

Power supply

AC100 [V], 50/60 [Hz] *2

Rated current

5 [A]Installation space
Internal temperature

Ensure space of at least 10 [cm] around the main unit

Setting range
Accuracy

Temp. display
Temp. correction

Safety devices

Output to external *9+3.0 - 10.0 [℃] *3±2.5 [℃] (at center of inside) *4
Digital display (1. internal temperature display, 2. flask interior fluid assumed temperature display) *3, 5

Other functions

Single point correction

Cooling type

Refrigerator (freon gas R-134a filling volume 170 [g])Mixing type

Forced mixing by internal fan motor

HEPA filter
Air flow
Manostar gauge
Internal dimensions
Outer dimensions
WeightStandard accessories

Electric leakage, overcurrent circuit breaker, fuse, anti-icing thermostat *6
Defrosting function (forced stop of refrigerator for approx. 15 [mins] once every 8 [hours] approx.)

Other functions

Automatic dew condensation water evaporation function

Hose holder for O.D. φ6 (installed at the bottom right side of interior entrance)
Left side surface of the unit has a φ36 connecting hole to the inside.

*1
*2
*3
*4
*5
*6

26

Low-temperature Incubator
SLI-4090-B

Low-temperature Incubator

Name
Model

Safety devices

Name

Usage conditions*1■ SLI-4090-C
Item

Temp. control

Specifications

Model

SLI-4090-C

Usage conditions

Item

Internal dimensions

W505×D386×H461 [mm]Outer dimensions

W600×D696×H643 [mm]WeightApprox. 90[kg]Standard accessories

Power cable x 1 / fuse x 1 (in receptacle) / light plug x 1

Must be no condensation in main unit. May not fulfill product specifications at temperatures other than the above environmental temperatures.
Voltage fluctuation tolerance ±10 [%]At shipment, the internal setting temperature are adjusted so that the assumed temperature of the liquid in the flask is within +6.0±1.0 [℃] (@19.0 [℃]).
In the environment temp. 19 [℃]. This does not include temperature changes due to the defrosting function.
The assumed temperature of the liquid in the flask is the temperature of the measuring rod located at the upper left rear corner of the chamber.
Activates when an irregular low temperature is detected. Forces the cooler to stop when it activates (with automatic reactivation)

*1
*2
*3
*4
*5
*6
*7
*8
*9

Environment temp. range+18 - 22 [℃] *2
Power supply
AC100 [V], 50/60 [Hz] *3
Rated current
5 [A]Installation space
Ensure space of at least 10cm around the main unit
Internal
Setting range+3.0 - 10.0 [℃] *4
Accuracy±2.5 [℃] (at center of inside) *5
temperature
Digital display (1. internal temperature display, 2. flask interior fluid assumed temperature display) *4, 6
Temp. display
Single point correction
Temp. correction
Refrigerator (freon gas R-134a filling volume 170 [g])Cooling type
Forced mixing by internal fan motor
Mixing type
Electric leakage/overcurrent circuit breaker, fuse (built-in), sensor disconnection,
sample protection high/low temperature alarm (factory settings *7), safety device for built-in HEPA filter(Circuit protector 1 [A], high temperature safety device 90 [℃])
Anti-icing thermostat *8

Temperature
Pt100 [Ω] sensor (-20 - 50 [℃]: 0 - 5 [V])High/low temp. alarm output (normal: open, error: close / AC250 [V], 1 [A] resistive load)
Alarm
Defrosting function (forced stop of refrigerator for approx. 15 [mins] once every 8 [hours] approx.)
Automatic dew condensation water evaporation function
Hose holder for O.D. φ6 (installed at the bottom right side of interior entrance)
Left side surface of the unit has a φ36 connecting hole to the inside.

Low pressure loss HEPA filter 400 × 400 × t50 [mm]Approx. 3.0 [㎥/min]
Pressure range 0 - 300 [Pa]W505×D386×H461 [mm]W626×D696×H830 - 900 [mm]Approx. 110 [kg]Power cable x 1 / fuse x 1 (in receptacle) / light plug x 1 / temperature sensor test report x1

Cleanliness class of the installation environment is ISO class 7 or higher.
Must be no condensation in main unit. May not fulfill product specifications at temperatures other than the above environmental temperatures.
Voltage fluctuation tolerance up to ±10 [%] When decontaminating the room, turn off the breaker of the main unit and put the cover to the refrigerator air inlet.
At shipment, the internal setting temperature are adjusted so that the assumed temperature of the liquid in the flask is within +6.0±1.0 [℃] (@19.0 [℃]).
In the environment temp. 19 [℃]. This does not include temperature changes due to the defrosting function.
The assumed temperature of the liquid in the flask is the temperature of the measuring rod located at the upper left rear corner of the chamber
Sample material protect alarm setting: low temperature -5 [℃]/high temperature 20 [℃]
Activates when an irregular low temperature is detected. Forces the cooler to stop when it activates (with automatic reactivation)
2[m] cable from main unit, cable terminal: Y terminal
・High/low temperature alarm (normal: open, error: close (line color: black/green))
・External temperature data (0 - 5 [V], line color: red/white)

27

Option parts

Option parts

Optional items that can be connected to Satake BioReactors■ Cooling water circulator - Satake Cool Ace SCA-32■ Aeration unit

The Cool Ace is a cooling water circulator that uses
a refrigeration unit to chill the liquid in the included
reservoir for external circulation, cooling eachcomponent that generates heat, such as the evaporator(1 L), culture tank, reaction tank, and various analyticaldevices and other equipment.

This unit is equipped with a compressor for easy air
supply to a compact culture tank.
【Feature】
・Compact size for easy installation even in narrow
spaces.
・L i g h t w e i g h t f o r a i r t a n k - s t y l e i n s t a l l a t i o n s ,eliminating the need to find installation locations.

■ Aeration unit

【Feature】
・It is a compact, tabletop type that can be installedeven in narrow spaces.
・In addition to cooling the heat producing componentsof the BioReactor, it can be used for a variety of otherpurposes, such as analytical equipment.
・T emperature control is achieved with a simple
panel.
・T emperature settings range from -20 to 30 ℃ forversatile applications.
・The circulation nozzle can be moved in 360°, reducing
the load on tubes.

Specifications

Item

Name
Aeration Unit
Model
SAU-3505
SAU-3525
SAU8050
SAU80100
Flow meter*
50 - 500 [NmL/min]
0.2 - 2.5 [NL/min]
0.5 - 5 [NL/min]
1.0 - 10 [NL/min]
Rated pressure
6.86×104 [Pa] (0.7 [kgf･cm2])
9.81×104 [Pa] (1.0 [kgf･cm2])
Performance
Compressor Rated flow rate
3.5 [L/min]
8 [L/min]Max. pressure
9.81×104 [Pa] (1.0 [kgf･cm2])
1.47×105 [Pa] (1.5 [kgf･cm2])
Flow rate adjustment
Constant flow valve for secondary pressure fluctuation
Configuration
Air outlet
O.D. φ8 [mm]Outer dimensions
W136×D306×H236 [mm]W136×D306×H301 [mm]Power consumption
0.25 [A], 25 [VA]
0.6 [A], 60 [VA]
OthersPower supply
AC100 [V], 50/60 [Hz]WeightApprox. 6 [kg]Approx. 8 [kg]Remarks
Needle valve (air relief valve) built-in*[NmL/min] or [NL/min] indicates the flow rate converted to 0 [℃] and 1 [atm] (101.3 [kPa])【Use Case】
Culture tank, cooling tube attached to reactor tank,culture tank temperature controller, viscosity gauge,etc.
■ Cooling water circulator■ Single-use pressure control system
This pressure controller enables culture tank pressure
control at a constant level.
【Feature】
・It can be connected to a glass culture tank or singleuse culture tank and the digital pressure switch
regulates the electromagnetic valve for maintaining
the culture tank pressure at a constant level.
・Fine tuning the opening of the manual bypass valve
controls hunting, enabling mild control.
■ Pressure control system

Specifications

Name

In-vessel pressure controller

Model

Si-P1

Display range

Pressure: -10.00 - 10.00 [kPa], Display accuracy: ±2 [%] (±1 [digit] F.S.)

Setting range

Pressure: -10.50 - 10.50 [kPa]

Output

MTA of outer surface
Installation

Outer dimensions
Usage conditions
Utilities

28

Specifications
Cooling water circulator SATAKE Cool Ace

Model

SCA-32

Circulation type

Closed system directional circulation

Temp. control range
Temp. accuracy
Performance

Cooling
Circulation

ON/OFF control-10 [℃]：310 [W] 10 [℃]：450 [W] 0 [℃]：350 [W]

Max. flow rate

9 [L/min] / 10 [L/min] (50/60 [Hz])Temp. setting/display
Safety functions

Temp. control
Temp. sensor
Configuration

Setting -1 - -20 [℃]: ±2 [℃], setting 0 - 20 [℃]) ±1 [℃]
4.2 [m] ±0.5 / 5.6 [m] ±0.5 (50/60 [Hz])Temp. control type

Function-20 - 30 [℃]Max. pump head

Optional functions

Item

Control type

Item

Name

Freezer ON/OFF control

Touch panel input configuration, digital switching displayof measured temperature or setting temperature (resolution: 1 [℃])Electric leakage/overcurrent circuit breaker, freezer overload relay,
self-diagnostic functions (freezer error, sensor error, watchdog timer),freezer protection timer, circulation pump impedance protection
Flow control valve, metal nozzle, cool keeping hose set, trolley, product fixing parts,
cooling water communication cable, communication cable (Linked with NVC-3000)
Touch panel input configuration and digital display
Pt sensor

Freezer

Air-cooled type, 450 [W], HFC, R-404A

Water tankTotal capacity approx. 3.2 [L], Actual capacity approx. 2.7 [L] Material SUS304

MTA of cooling coil

SUS316L

Circulation nozzle size

O.D. 10 [mm] × I.D. 6.5 [mm]Analog signal: 1 - 5 [V]Tank inner dimensions
Usage condition

5 - 35 [℃]

Tabletop

Outer dimensions

W205×D405×H545 [mm]Pressure resist: 50 [kPa], temperature: 5 - 45 [℃], humidity: 20 - 85 [%] RH (No condensation)

WeightApprox. 28 [kg]Power consumption

SUS304 (no coating), indoor type, non-waterproof, non-explosion proof specification
W125×D225×H100 [mm]Connection port :φ4 one touch tube fitting

Power supply

W130×D230×H115 [mm]8 [A], 800 [VA]

AC100 [V], 50/60 [Hz]System development and customization for demand specification are also possible.
Please contact our Bioprocess Equipment Division below for more information.
Contact number +81-48-471-9202 e-mail address：bio@satake.co.jp

29

Example of option system

Example of option system
Single-use

Cultivation, cleaning, and concentration in a single line!
Cell concentration cleaning system

Example of option system

As an improvement on the cell culture system, we sell
a connected, completely closed system that consistsof our reactor and attached Kaneka Corporation cell*1concentration cleaning system. This system can becustomized upon request.
From culture to cell washing and concentration, thisproduct enables consistent processing in a closed system,
streamlining the work process. A pump is built into
the cell wash concentrator body, making preparations
unnecessary, enabling simple installation by following
the pictures and colors on the accessories and body.

Single-Use Bioreactor

Single-Use Bioreactor

000

000

00

00

KANEKA Cell Washing Concentration System*1 Cell Washing Concentration System is KANEKA CORPORATION
made product for regenerative medicine.

■ Disposable Kit for

Disposable Kit

Cell Concentration Washer

―

―

Cell strainer

Circulation circuit

Filtration circuit● Sterilization method
Filter : γ sterilization
Circuit : EOG sterilization● Safety
Passed the test specified
by ISO10993(Cytotoxicity,
sensitization, intradermal
reaction, acute toxicity,hemolysis, pyrogenicity)

Please contact the following contact window when you purchase.
Biomaster, Inc. TEL : +81-45-222-3363■ Cell washing concentration system
Item

Example of system up

Name
Model
Flow rate range
Pump

Accuracy
No. of rollers
Applicable tube

P2

P1

PS1

BD3

PS2

Valve

Pressure sensor
BD1

Display
BD2

Wet classifier for lab

30

V3

KANEKA Cell Washing Concentration System

Outer dimensions
WeightPower supply
Water proof protection class
Environment temperature
Environment humidity
Fluid temperature
External color

No. of unitsType
Applicable tube
No. of unitsType
Measuring range
Accuracy
FittingType
Operational method

Specifications[Cell Washing Concentration System] Tubing Pump System
R-CS-S
20 - 500 [mL/min] (depending on fluid temperature)[Circulation pump]
20 - 350 [mL/min] (depending on fluid temperature)[Feed pump / Drain pump]±10 [%]
2[Circulation pump][Feed pump / Drain pump]

Pump diameter
φ80 [mm]
Clearance control
Automatic adjustment
φ8.00 × φ12.00±0.15 [mm] (PVC based)
φ6.40 ×φ 9.50±0.15 [mm] (PVC based)3

Normally close
Shut-off pressure
750 [mmHg] and more
φ3.50×φ5.50 [mm] /φ3.00×φ4.30 [mm] (PVC based)
4
Pressure transitor-750 - 750 [mmHg] (pressure gauge)±5 [%]
Luer lockTFT color LCD

No. of units
2
Effective display dimensions
116×87 [mm] (5.7 inch)
Touch panel (analog resistive film type)
W450×D400×H695 [mm]43 [kg]AC100 [V], 50/60 [Hz], 360 [W] (cable length: 2 [m])IPX1Operation: 15 - 35 [℃] / Transportation: 0 - 50 [℃]
Operation: 35 - 85 [%] RH with no condensation / Transportation: 30 - 90 [%] RH with no condensation
15 - 35 [℃]
SBY-S white (matte) / SBY blue (matte)

31

We are constantly committed to improve the quality of our products, thereby the design and specifications of our products may differ from those shown in the
catalog. Please understand this in advance.

Bioreactor exports from Japan fall under paragraph 3-2 (2) 2 of the Appendix Table 1 of the Export Trade Control Order, and products whose tank capacity is equal
to or more than 20L are subject to the regulation.(as of February, 2024)
In addition, export to users that listed in catch-all regulations is prohibited.
When you export bioreactors, please confirm the latest laws and regulations of export country.

We dedicated to manufacture products that satisfy our customers and are safe to use.

SATAKE MultiMix Corporation
Bioprocess Equipment Division :Room 502, Saitama Industrial Technology Center, SKIP City,
3-12-18 Kami-Aoki, Kawaguchi-Shi, Saitama 333-0844, Japan				
Phone：81-48-471-9202
E-mail：bio@satake.co.jp

Product

Bio Top (JPN)

https://www.satake.co.jp/
en/product/cultivate/https://www.satake.co.jp/
bio/(Overseas distributor)
TAITEC CORPORATION : 		2693-1, Nishikata, Koshigaya City, Saitama 343-0822 , Japan				Phone : 81-48-988-8371’24.3.5H(RKSL)10C8D
Printed in Japan