The CardioExcyte 96 SOL

Optogenetics meets cardiac safety• Integrates seamlessly with the CardioExcyte 96 and FLEXcyte 96• 96 channel optical stimulation• Spatially uniform stimulus allows mechanistic insights into contractility
and electrophysiology of iPS cardiomyocytes• No stimulus artifacts• Pacing without electrical stress for cells, allowing long-term
stimulation during maturation cycles

CardioExcyte 96 SOL
Optical stimulation and investigation of impedance,
extracellular field potentials or contractility in parallel

The CardioExcyte 96 is a hybrid device supporting highly

The stimulating optical lid, CardioExcyte 96 SOL, uses

resolved impedance-based measurements, MEA-like

LEDs for spatially uniform stimulation of cells transfected

extracellular field potential (EFP) recordings and true

with light-gated ion channels such as Channelrhodopsin2

contractility (FLEXcyte 96 Add-On)). It has a small(ChR2).

footprint and allows for fully automated recordings from
96 wells in parallel. With embedded control electronics
and sophisticated sensor technology, the CardioExcyte
96 is a turn-key system for efficient measurements ofbeating networks of e.g. iPS cardiomyocytes.

Optogenetics
After transfection of iPS cardiomyocytes with ChR2 thecells were stimulated by blue light. Every flash of light
induces a depolarization of the cells and thus an action

CardioExcyte 96 SOL

Nanion EuropeNanion USA

Nanion China

Nanion Japan

info@nanion.de

info@naniontech.com

andy.di@nanion.cn

ohtsuki@nanion.jp

phone: +49 89 2 190 95-0

phone: 1-888-9-NANION

phone: +86 10 82 17 63 86

phone: +81 3 6457 8776

www.nanion.de			potential is triggered. The advantages of opticalstimulation over electrical stimulation include the highly

Timed stimuli allow exact IMPand EFP mean beat overlays

precise timing, all cells of the beating network are

100 nM Nifedipine (black) shorten
the FPD and reduce contraction
strength, 2 Hz paced cells

stimulated exactly at the time of the light stimulus. In
contrast, electrical stimulation propagates from theEFP

electrode across the well and thus cells are stimulated
progressively. A mean beat calculation of precisely
timed beats enables in-depth compound analysis andIMP

concentration response dependencies to be obtained.
This is one of the key software capabilities of theCardioExcyte Control/ DataControl software package

Nifedipine
1030100300 nM

Dofetilide
31030100 nM

that is provided with each CardioExcyte 96 system.

control
2 Hz stimulated Cor.4U/ChR2 cells in control and in the presence ofNifedipine and Dofetilide. Impedance amplitudes were reduced in aconcentration dependent manner. Red traces: before dosing.

ChR2 transfected Cor.4U cells kindly provided by Ncardia

CardioExcyte 96 system with Integra Assist pipetting robot

CardioExcyte96 SOL
Technical details:• Allows for in vitro optogenetic assays• Add-on for the CardioExcyte 96 /FLEXcyte 96• Integrated in the lid of the incubation system• 96 LEDs - each well is stimulated by an individual LED• High power LEDs coupled with custom lid optics forrobust performance and reliability• Software controlled adjustment of stimulus intensity,
pulse length and pace rate
• Optical wavelength 470 nm, for ChR2, other wave
lengths available upon request
OPTEL Consortium funded by EuroTransBio Initiative and BMBF.
Consortium consists of Inst. für Physiologie I, Univ. Bonn, Life and Brain
Center. Axxam S.p.A, Milan, Italy. Nanion Tech., Germany.

The CardioExcyte 96 with a Nanion NSP - 96 sensor plate

CardioExcyte 96 SOL software integration
The optical stimulation option is fully integrated in theCardioExcyte Control software. Our data analysis
approach enables an exact alignment of both
impedance and EFP traces with the light stimuli. Opticalstimulation can be switched on or off individually for asubset of wells.

Furthermore, cells can be either

continuously stimulated or specifically timed, dependingon the experiment. A setup that provides high flexibility!

CardioExcyte96 SOL