Agilent MassHunter Profinder Software

ATTAIN SUPERIOR FEATURE EXTRACTION

ACHIEVE HIGHER QUALITY RESULTS

Agilent MassHunter Profinder software
provides targeted and untargeted molecularfeature extraction (MFE) for batches ofmass-spec data—not just one file at atime—and is designed to accelerate yourdifferential analysis workflow.
Profinder’s MFE algorithm extracts
data based on mass spectral andchromatographic characteristics to generate
a complete list of compounds, which
includes molecular weight, retention time,
m/z, and abundance—and it has a recursivemode to address missing features. This
batch-recursive mode first bins and aligns
compound features found in a first-pass
MFE, then uses those results to create alist for a targeted second pass.

Intuitive and easy to use,
Profinder offers an array ofuseful features:If you know your compounds of interest,
Profinder’s targeted feature finding
algorithm will extract compounds of interest
with known chemical formulas from large
complex data sets. This workflow offers
higher selectivity, faster data processing,and tentative compound annotation.

• Binning and alignment

Agilent MassHunter Profinder Workflow

1. Samples2. LC/MS or GC/MS

3. Profinder

4. Results2• Grouping of replicate data files• Chromatogram overlay• Manual re-integration of compounds• Export of results
Profinder supports MS-only datafrom Agilent’s broad portfolio of mass
spectrometers including TOF-based
instruments (with GC, LC, CE or SFC) as
well as nominal mass GC/MS systems.

Extra performance forfast results
Even with an advanced algorithm,
sometimes user intervention is required.
Profinder provides data visualization acrossall files and a manual integration tool thatenables visual validation of the feature
extraction results.
Profiling experiments can be quite large,
presenting a computational challenge forany feature extraction software. Profinder’sfeature-finding algorithms are multithreaded
to take advantage of common multicore
Intel processor configurations—and its
memory management optimized to runlarge datasets in the Microsoft’s 64-bit
Windows operating systems.
Profinder simplifies connecting results fordownstream analysis by offering multiple
export options. A common format (.CSV)
is supported to allow you to interface toany software package. In addition, Agilent

created a richer single data file format(.CEF) to support enhanced informationexchange with other Agilent-supported
software applications. Profinder also offers
a rich multiple data file format (.PFA)
for transferring results to batch-oriented
software applications such as OmixPremium.

Workflow solutions
Profinder provides workflow solutions forsmall molecule profiling, intact protein
profiling, and isotopologue profiling. Small
molecule profiling is useful for a wide range
of applications including the analysis offoods, beverages, wastewater, polymers,and metabolites. Profinder includes a special
algorithm for intact protein profiling designed
for multiply-charged protein spectra. Protein
profiling can be of use in food analysis as
well as basic protein research.

Profinder supports qualitative flux analysis
based on data acquired on Agilent highresolution, accurate-mass, time-of-flight
LC/MS systems. The algorithm mines forstable isotope-labeled ions (isotopologues)
and supports 13C, 15N, or 2H stable isotope
labeling. Profinder provides a batch format
result output (.PFA) for Agilent MassHunter
VistaFlux software.

“In my lab, MassHunter Profinder is already our tool of choice foruntargeted analysis of TOF and Q-TOF data. Profinder’s powerfulalgorithms sit behind a biologist-friendly interface that lets
students, post-docs, and visiting scientists focus on sample
collection and interpretation. I’m particularly excited by theaddition of isotopologue analysis to the latest version. It’s been
great to see individual members of the lab empowered toanalyze their own kinetic flux profiling data.”
ADAM ROSEBROCK, PHD, UNIVERSITY OF TORONTO
3

Learn more
www.agilent.com/chem/profinder
Find an Agilent customer center
www.agilent.com
U.S. and Canada
1-800-227-9770
agilent_inquiries@agilent.com
Europeinfo_agilent@agilent.com
Asia Pacificadinquiry_aplsca@agilent.com
For research use only. Not for use in diagnostic procedures.
This information is subject to change without notice.
© Agilent Technologies, Inc., 2016
Published in the USA, August 4, 2016
5991-6984EN