Focus on Simplicity
Next-generation HT custom CGH microarrays

Key features–

Reliable — custom microarrays basedon the Agilent SurePrint technology
for results you can trust–

Focused — tailored content definedby the user starting from catalog
probes for more targeted analysis–

High throughput — new high-plex
format to provide higher throughput
with a lower per-sample cost–

Streamlined — seamless workflow
from sample to results for fasterturnaround time–

Simple — new labeling kit andHybStation enable easier sample
processing

Focus on what matters with custom SurePrint microarrays fortargeted analysis
The new Agilent SurePrint HT CGH microarrays, in three custom microarray
formats (16x CGH, 16x CGH+SNP, and 24x CGH), reinvent microarraysby enabling high-throughput analysis of targeted genomic regions. The
SurePrint HT CGH arrays allow focused analysis of many samples, witha quick turnaround time and a reduced cost. These arrays are available
for comparative genome hybridization (16x and 24x CGH) and loss ofheterozygosity (16x CGH+SNP).
Content can be easily defined by the user starting from our online probe
database. Cover the whole genome at high resolution, select a subset ofprobes from our catalog design, or include newly discovered, sequenced
genome regions all with few easy steps.

Figure 1. The new Agilent SurePrint HT CGH microarrays include a 16x 25k CGH and CGH+SNP option for low complexity content such as whole-genome
screening, and a 24x 13k CGH option for high throughput of focused content such as high-resolution copy number screening.

A trusted, fast, flexible solution for your research
The SurePrint HT CGH microarrays are part of the Agilent microarray workflow, offering a complete solution from sample toresults, featuring a new dedicated HT labelling kit, gaskets, Hyb Station, and full compatibility with existing hardware.

Figure 2. The Agilent SurePrint HT CGH microarray workflow, from sample to results.

Workflow benefits include:
Flexibility: use your existing Agilent SureScan and SureScanDx, HybOven, chambers, and HybStation without the need toupdate your hardware.
Simplicity: use the same reagents as your existing CGH arrays for easier order management.
Compatibility: arrays are compatible with the Agilent Feature Extraction 12.0 and CytoGenomics 5.3 software
Efficiency: bead-based purification is automation-ready and facilitates streamlined processing of multiple samples withhigh reproducibility.
The new Agilent SureTag HT labelling kits offer improved, simplified purification using proprietary nano beads from Agilent
and require no speed-vacuum step, enabling efficient processing and adherence to volume requirements. These are ready for
implementation on an automated liquid handling platform for increased throughput and improved reproducibility. The kit will beavailable in two formats, with and without reference DNA included, and enough reagents to process 96 samples for both 16x and24x arrays.

Figure 3. On chromosome 3 (Chr3), this sample displays a 74.8 Mb deletion (3p25.3p12.1) with a log2 ratio of -0.27, indicating that this deletion occurs in a portion of
the tumor cells. The SNP probes confirm the deletion. In addition, it displays a 35.5 Mb amplification (3q24q26.33) also confirmed by the SNP probes.

2Figure 4. Gene view in cytogenomics of a Breast FF sample tested both on 16x and 24x CGH designs. In the top panel is visible a 4 Mb window around a CNV(deletion) on Chr2. Visible in the bottom panel is a zoom-in around gene NPHP1, deletion is detected by both designs.

Improve your workflow with a stress-free sample processing
platform
Process your samples with ease and achieve reproducible results while
lowering training needs with the Agilent HybStation, a new sample loading
platform that provides a consistent and stress-free microarray sample
preparation process.
–Designed to provide stability, the platform minimizes errors during each
step, addressing the variability of the array loading step where the risk isgreatest–

Intended to assist with assembly of Agilent microarray hybridizationchambers prior to incubation in a hybridization oven, increasing simplicity
and reproducibility of the process

3Figure 5. The Agilent HybStation provides aconvenient and stable platform to efficiently
process your microarrays.

Tailored content for focused
analysis
Two new microarray formats, 16x and 24x(Figure 1), can be designed easily throughour online web tool selecting regions ofinterest from existing catalog content, orusing the design wizard capability to guide
you through the design process. For more
info visit https://earray.chem.agilent.com/
suredesign.
The three new arrays provide a unique
combination of throughput, content, andprice, filling the gap between multiplexing
PCR (not enough data points) and NGS/
microarrays (expensive/complex).
Slides have been optimized to get
excellent balance between on-slide
multiplexing and content for maximum
flexibility in customer designs.

SurePrint HT CGH Microarrays technical specifications
16x CGH

16x CGH + SNP

24x CGH

Controls

1,881

1,881

1,881Normalization Controls

1,262

1,262

1,262

Replicate Probe

1,000

1,000

1,000

CGH or CGH+SNP

21,657

22,919*

8,937

Total

25,80025,80013,080Ordering information
Part Number

Product Name

G4133A

SurePrint G3 Custom HT Microarray CGH 24x13k

G4132A

SurePrint G3 Custom HT Microarray CGH 16x25K

G4132B

Sureprint G3 Custom HT Microarray CGH+SNP 16x25K

G9978B

SureTag HT CGH labelling kit with SurePure Beads

G9978A

SureTag HT Complete CGH labelling kit with SurePure Beads

G2534-60024

Pack 100 Backings 24 Arrays per Slide, to be used with HT Microarrays

G2534-60023

Pack 20 Backings 24 Arrays per Slide, to be used with HT Microarrays

G2534-60022

Pack 5 Backings 24 Arrays per Slide, to be used with HT Microarrays

G2534-60021

Pack 100 Backings 16 Arrays per Slide, to be used with HT Microarrays

G2534-60020

Pack 20 Backings 16 Arrays per Slide, to be used with HT Microarrays

G2534-60019

Pack 5 Backings 16 Arrays per Slide, to be used with HT Microarrays

G5765A

Hyb Station Platform

Additional reagents

www.agilent.com

For Research Use Only. Not for use in diagnostic procedures.
PR7001-1571
This information is subject to change without notice.
© Agilent Technologies, Inc. 2023
Published in the USA, October 30, 2023
5994-6481EN

Part Number

Product Name5190-0402

Oligo aCGH Prehybridization Buffer

5188-5226

Oligo aCGH/ChIP-on-chip Wash Buffer Kit