Compact DryTMCF Coliform bacteria test dish

 Coliform colonies appear blue~Blue green
 Culture conditions: cultured at 35 °C ± 2 °C for 24 hMainingredients:

Chromogenic medium of containing enzyme substrate

Storageconditions:

Room temperature (1-30 °C)

Shelf life:

18 months

Strengths

Compact Dry CF 40 pieces / box Code 06744
Compact Dry CF 240 pieces / box Code 06745
Sterile homogenized bag (with filter membrane) 500 / box Code 01540
Sterile homogenized bag (without filter membrane) 1000 / box Code 01541

Compact Dry test dish is produced using
the unique patented technology of SDC

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Copyright © Shimadzu Diagnostics Corporation. All rights reserved.

Compact DryTMCF
Coliform bacteria test dishInterpretation
manualThe Nissin Compact DryTM CF coliform test dish is a pre-prepared chromogenic
medium containing an enzyme substrate.
The growth of typical colonies
Coliform bacteria number = 155
The test dish contains a color indicator tomake the colony show blue~bluey-green

Coliform bacteria number = 0
There is no colony growth on the test dish.

There is always a better way.
Copyright © Shimadzu Diagnostics Corporation. All rights reserved.

Compact DryTMCF
Coliform bacteria test dishColiform bacteria number = 17
There is a small amount of colony growthon the test dish.

Coliform bacteria number = 160
There are many colonies growing on thetest dish. A reasonable count range is15-150.
There are too many colonies and further
dilution is required to get an accurate
count.

There is always a better way.
Copyright © Shimadzu Diagnostics Corporation All rights reserved.

Compact DryTMCF
Coliform bacteria test dishNumber of coliforms = Too Numerous ToCount (TNTC) (estimated value 103) The
number of colonies on the test dish iscounted as TNTC.
There are too many colonies and further
dilution is required to get an accurate
count.

Number of coliforms = irreducible (TNTC)(estimated value 104) The DE colonies on
the test dish are all stained, and thenumber of colonies is counted as TNTC.
There are too many colonies and further
dilution is required to get an accurate
count.

There is always a better way.
Copyright © Shimadzu Diagnostics Corporation All rights reserved.

Compact DryTMCF
Coliform bacteria test dishThe area of the test dish is 20 cm2, when
the number of coliform bacteria number
exceeds 150. In order to estimate thenumber of colonies, selecting one orseveral representative small squares,and calculating the average number ofcolonies, and then multiplied by thecorresponding multiples to obtain thenumber of colonies of the entire test dish.
Coliform bacteria number (> 150)
= average number of colonies per cell
(1cm * 1cm) * 20
= average number of colonies per cell
(0.5cm * 0.5cm) * 80

Tips for opening the cover:Press the test tube with the thumbjoint and lift the fingertip from thebottom of the edge of the lid and lift
it up. This makes it easy to open thelid.

There is always a better way.
Copyright © Shimadzu Diagnostics Corporation All rights reserved.

Compact DryTMCF
Coliform bacteria test dishInstructions

Sample Preparation

Sample dilutions of 1:10 or

Add the appropriate amountgreater dilution factor areprepared. Weigh or grab thefood sample and place it ina suitable sterile container.

of sterile diluent.

Stir or homogenize the sample.

Sterile dilutions include:
Phosphate buffer or physiologicalsaline (GB4789), 0.1% peptonewater, peptone saline dilution (ISOmethod 6887), buffered peptonewater (ISO method 6579),
bisulfite-free Letheen broth or
distilled water, and the like.

For example, the sample diluent isadjusted to pH 6.5-7.5
Acidic samples are adjusted with
1N NaOH
Alkaline samples are regulatedwith 1N HCL

Inverted into an incubator andincubated at 35 °C ± 2 °C for
24 h.

Pour it on a white background ortranslucent plate, and count it witha visual or colony counter.

Vaccination culture

1 mL of the sample solution is
inoculated in the center of thetest dish, and the sample
solution is uniformly spread
uniformly around the plate.
(The medium area is 20cm2)

There is always a better way.