大 学 の 世 界 展 開 力 強 化 事 業「ASEAN 諸国との協働・連携による次世代医学・保健学グローバルリーダーの育成」

1043818ｍ

高坂綾香

研修期間（6/3～7/21 ヶ月間）

I have attended a training course about a variety of bacteria and cases in Airlangga
University, Indonesia between June 3 and July 2. I report about the course as follows.
[Training in the laboratory of Intestinal tract infectious diseases ]I had trained for a total of 13 days at the laboratory of intestinal tract infectious
diseases in the University. In the laboratory, The Indonesian staffs isolated clinicalstrains such as Salmonella spp., Vibrio spp and Escherichia coli, and performed genetic
analysis and drug susceptibility testing in the labolatory. In the first two weeks, I
actually experienced to isolate using the test medium, and perform the drug
susceptibility testing and the DNA extraction from the stool specimens of pediatricpatients with diarrhea that has been sent from Soetomo hospital . I also performed toisolate Salmonella spp. from chicken samples in the last week.
In the training, I found that E. coli and Klebsiella pneumoniae were isolated from mostof stool specimens of diarrhea patients in the labolatory. In particular, I was surprised
the previous data indicated the isolation of Shigella spp.. Furthermore, I detectedadenovirus, rotavirus and norovirus using Japanese-made adenovirus, rotavirus andnorovirus detection kits. I first taught the Indonesian staffs how to use these kitsalthough I always learned the Indonesian methodologies for the bacterial isolations bythem, Of course, It was very difficult for me to teach them in English, but I wassurprised the staffs consciously asked detailed questions about the kits. Although theyhad willingness, they perfectly couldn’t use the Japanese virus detection kits because
there were no Japanese resident researchers, I felt like a contradictory situation
TheIndonesianmethodologies

weremostlyconsistentwiththeJapanesemethodologies, but I realized it was slightly different between Indonesian andJapanese methodologies. The differences were the waysof directly putting stool

specimens on the medium, using an alcohol lamp and make the medium using glass
dishes. I always felt the importance of contamination in our laboratory. According tothe Indonesian condition, I became more aware of the high risk of the contaminationin the Indonesian laboratory compared to the Japanese laboratory.
[Human immunodeficiency virus (HIV) laboratory tour]
I had a tour of research facility, which newly has a human immunodeficiency virus(HIV) laboratory, in the University for a day. Moreover, it was also planned that I took
part in blood samplings for HIV patients who were drug users. I shocked the activity ofthe samplings for the HIV patients because I have been seen it in Japan. But thesamplings had to be canceled.

[Influenza virus laboratory tour]
I also had a tour of Influenza virus laboratory in the research facility for a day. The
laboratory has been conducted clinical and epidemiological studies. The staffs isolated
the influenza virus from serum of birds, and performed RNA extraction and thegenotype analysis, I looked around the experiments to detect genes of influenza virus
from the serum of birds by RT-PCR in biosafety level (BSL)3 room because of thepossibility that high-risk type was included among the virus. It was a very goodexperience because I had been never entered the BSL3 room. To enter the room, we had
to wear the gown covered the entire body except eyes, double groves and goggles forprotecting our bodies. Thus, It reminded me how dangerous influenza virus was.
Additionally, I watched a Japanese staff explained a procedure of the study, andIndonesian staffs aggressively confirmed and performed it. I really felt a goodcollaboration between Indonesian and Japanese staffs to perform it.
[Sutomo hospital tour]
I visited Sutomo hospital for only half a day. The hospital was the second largest inIndonesia, in particular, pediatrics had about 70 to 150 patients. There were many
patient rooms for infectious diseases such as pneumonia, dengue fever, tuberculosis
and liver diseases in the pediatrics., The 0-5 years and over 5 years children
hospitalized in the first and the second floors, respectively. There were almost 10-20
beds at each room. When the number of patients was too large, the staffs set up thebeds in the hallway. The rooms did not put up curtains as a partition in Japaneserooms. The patient's families sat on the bed, some peoples even ate on the bed. I alsowatched facilities which I had known such as a hospital school for long-term hospitalchildren, operating rooms, and intensive care units. I saw twin babies who were bornwith sharing stomach, children with a big head just containing water due tohydrocephalus, children being in the hospital for three years, and the baby whose bodycolor was yellow. After all I thought that the environment surrounding children inIndonesia is severer than that in Japan.
[My impressions]Through this training in Indonesia for a month, I got really good experiences because I
could do what I would not be able to do in Japan. It was especially impressive for me tovisit to Soetomo hospital. I was able to consider my current study, why the study wasneeded, how important the study was and what the study would lead to. In addition,
people who I met in Indonesia were very kindness. I made good relationships withthem. Therefore I also wanted to contact with foreigners like them. In Indonesia, I feltthat the number of saying “Thank you.” is larger than that of saying “I’m sorry.”

Indonesia with enriched kindness and love was very attractive. I could get various
knowledge and experiences and put them to use for my study and life.

・The drug susceptibility testing・The laboratory of Intestinal tract infectious diseases

・Soetomo hospital tour

・Using the kit in Japan to detect adenovirus

・The group of Flu.

・BSL３・My friends inIndonesia

・The gate of ITD