Tokuyasu Workshop 2019 ご案内
沖縄科学技術大学院大学(OIST)と順天堂大学（私立大学ブランディグ事業脳の機能と構造を視る：多次元イメージングセンター）との共催により、2019 年 1 月 21 日(月)〜25 日(金)に OIST において徳安法（凍結超薄切片法）のワークショップが開催されます。ワークショ
ップは午前の座学の講習と午後の実技講習に分かれ、全て英語で行われます。実技講習は凍結超薄切片作成、免疫反応、電子顕微鏡観察からなります。自身のサンプルと抗体をお持ちいただくことも可能です。
参加費は無料ですが、交通費と滞在費は参加者で負担願います。
実技講習は先着 12 名までとさせていただきますが、座学の講習のみの参加も可能です。
奮ってご参加ください。
本ワークショップについての問い合わせ、申込先
沖縄科学技術大学院大学(OIST)
イメージングセクション
担当者：渡嘉敷 瑞貴
E-mail: mizuki.tokashiki@oist.jp

Tokuyasu Workshop 2019
21st – 25th January 2019
OrganisersProf. Masato Koike, Juntendo University, Tokyo
Dr. Bruno M. Humbel, Okinawa Institute of Science and Technology
Venue
Okinawa Institute of Science and Technology
1919-1 Tancha, Onna-son
Okinawa, 904-0495
Japan
AimThe Tokuyasu cryo-sectioning method is a well-established technique in Cell
Biology for the localisation of macromoleculs by immuno-gold labelling. We
will teach the essentials like chemical fixation and its impact on the cellularultrastructure, how to judge the quality of antibodies and to do
immunolabelling. The delegates will gain some experience in cryo-sectioning,
however, need to practice in their own lab to get the necessary skills.
Interested delegates should apply with a short description of their current
project and motivation to attend the course. The lectures are open to anyone
but the practical part is limited to 12 participants.
Lecturers
Dr. Heinz Schwarz, Max-Planck-Institute for Developmental Biology, Tübingen,Germany.
Immunolabelling and Markers
Prof. Roland F. Fleck, King’s College, London, UK.
Cryo-Preparations and Cryo-SEM
Prof. Masato Koike, Juntendo University Tokyo, Japan.
Basic Sample Preparation for Tokuyasu Cryo-Sectioning
An Example of Application for Visualising Autophagy
Dr. Bruno M. Humbel, Okinawa Institute of Science and Technology, Onnason, Japan
Chemical Fixation
Correlative Microscopy

Application
For the application please state name, affiliation, name of supervisor, phone
number and e-mail address. Add a letter of motivation explaining your current
research project and why you want to attend the course. This letter will help
us to choose the applicants in case of oversubscribing.
The chosen delegates should prepare about 5 slides to introduce themselves
and the project at the beginning of the course
Delegates who only want to attend the lectures also need to apply as theseats in the lecture theatre are limited.
The application should be sent to Ms. Mizuki Tokashiki,Mizuki.Tokashiki@oist.jp.
Accommodation
Moon Beach: https://hotelmoonbeach.moonhotelsandresorts.com/
Sheraton Sun Marina: https://sheraton-okinawa.co.jp/
Kafuu: https://www.kafuu-okinawa.jp/en/
Rizzan: https://www.rizzan.co.jp/english/index.html
We made a preliminary reservation at hotel Moon Beach, Onna-son. Please
let us know when you would like to profit from it.
In principle we work with well-established course samples. In case you want
to bring your own, send a request stating the nature of the sample, data sheetof the antibodies, Western blots of the sample with the corresponding
antibody and fluorescence images.
Costs
Course participation is freeParticipants cover all other costs like transportation and accommodation
The course is supported byMEXT-Supported Program for thePrivate University Research
Branding Project (JuntendoUniversity)