豊田ホタルの里ミュージアム研究報告書 第 11 号 : 1-7 頁 , 2019 年 3 月
Bull. Firefly Museum of Toyota Town. (11): 1-7, Mar. 2019

Update on northernmost distribution of the luminous earthworm Microscolex phosphoreus(Dugès, 1837) in Japan, and their DNA and size variations
Seesamut TEERAPONG1,2,3), Ikuhiko KIN3), Panha SOMSAK2), Yuichi OBA3)
1)

Biological Sciences Program, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand

2)

Animal Systematics Research Unit, Department of Biology, Faculty of Science, Chulalongkorn

University, Bangkok 10330, Thailand
3)

Department of Environmental Biology, Chubu University, Kasugai 487-8501, Japan

Abstract: The luminous earthworm Microscolex phosphoreus was collected from Akita
City in Akita Prefecture (39º43'N). This is the new record of northernmost distributionof this species in Japan. We also collected the specimens again from Sendai City,
where the previous northernmost record in 2016, in Miyagi Prefecture. All specimens
collected in Akita City were in adult stage, while collected in Sendai City and Kasugai
City (Aichi Prefecture) at almost the same time contained both in juveniles and adults.
Key words: Bioluminescence, Northernmost distribution, Akita Prefecture, Microscolexphosphoreus.
INTRODUCTION
The luminous earthworm Microscolex phosphoreus (Dugès, 1837) is cosmopolitan (Rota et al., 2018), and the distribution inJapan has been recorded (Yamaguchi, 1935; Oba, 2012; Oba et al., 2011a, 2011b, 2016), especially in recent years, from thecentral region of Japan (Honshu, Shikoku, Kyushu). On the other hand, this species was not recorded from northern most area,
Hokkaido, and southern most region, Ryukyu. Previously, we reported the distribution of this species from the city parks ofYamagata City in Yamagata Prefecture (38º13'N) and Sendai City in Miyagi Prefecture (38º16'N) of Tohoku Region, where thefar north of Honshu (Oba et al., 2016). In this study, we reported a new record of the northernmost distribution of M. phosphoreus
from Akita Prefecture, and the comparison of their DNA and size variations to those of other localities.
MATERIALS AND METHODSThe earthworms Microscolex phosphoreus were surveyed from Aomori Prefecture, Akita Prefecture, Miyagi Prefecture
and Aichi Prefecture in November 2018. The specimens were collected by carefully digging up the topsoil near casts andby hand sorting. The worms were killed in 30% (v/v) ethanol, photographed and transferred to 99.5% (v/v) ethanol for longterm preservation, molecular analysis and subsequent morphological studies. The materials were deposited in Departmentof Environmental Biology, Chubu University. The number of segments, body length and diameter (at segment XX) of thecollected specimens were counted and measured under a stereomicroscope (WRAYMER LW-800 series). Only the adultM. phosphoreus were measured and used to analyzed, with juvenile from all sampling sites being exclude.
Total DNA was extracted from the posterior past of samples using Lysis Buffer for PCR (Takara). The fragment of1

S. TEERAPONG, I. KIN, P. SOMSAK, Y. OBA

mitochondrial cytochrome c oxidase I (COI) was amplified with the Tks GflexTM DNA Polymerase (Takara) using universal
primers (Folmer et al., 1994), as follows: 1 μl of Tks Gflex DNA Polymerase (1.25 unit/μl), 25 μl of 2x Gflex PCR buffer(Mg2+, dNTP plus), 1 μl each of LCO1490 (forward) and HCO2198 (reverse) universal primer, 19.5 μl of sterilized distilledwater and 2.5 μl of crude lysate with Lysis buffer. The PCR reaction mixtures were heated to 94 °C for 2 min, followed by 35
cycles of 94 °C for 1 min, 48 °C for 1 min, 72 °C for 2 min and a final extension at 72 °C for 5 min. The concentration andquality of the amplicons were tested with 1% agarose gel against a DNA standard marker in 1x TAE buffer and detected under
UV transillumination. For sequencing, the PCR products were directly sent to Macrogen Inc. (Japan) without purification. All
COI sequences were aligned using the ClustalW algorithm in MEGA7 v. 7.0.18 (Thompson et al., 1994; Kumar et al., 2016)
and manually checked by eyes. The sequences were checked and compared with other sequences available in the GenBankdatabases at the National Center for Biotechnology Information (NCBI) using BLAST similarity search tool (http://www.ncbi.
nlm.nih.gov).
RESULTS
In late November 2018, luminous earthworm Microscolex phosphoreus surveys were conducted in 4 locations in Japan(Aomori prefecture, Akita Prefecture, Miyagi Prefecture and Aichi Prefecture). In our field study, we did not get specimens inHachinohe, Aomori. The detailed distribution of the habitats and the collection information of collected earthworms
M. phosphoreus in 3 locations are given below.
Locality: North side of Research Center of Advanced Materials for Breakthrough Technology, Akita University Campus, 1-1-1
Hondo, Akita, Akita Prefecture, Japan (39º43'N, 140º08'E).
Date: 21 and 22 November, 2018

Figure 1. Photographs showing (A) Microhabitat of M. phosphoreus at Akita University Campus,
Akita Prefecture; (B) M. phosphoreus in the sampling site in Akita Prefecture; (C) Bioluminescence of M. phosphoreus collected from Akita Prefecture.

2Update on Northernmost Distribution of the Luminous Earthworm Microscolex phosphoreus (Dugès, 1837) in Japan

Collectors: Yuichi Oba and Kenshu Fujiwara
Number of samples: 21 adults
Locality: Yanagimachi Park, 1-14-23 Ichiban-cho, Sendai, Miyagi Prefecture, Japan (38º15'N, 140º52'E).
Date: 30 November, 2018
Collectors: Seesamut Teerapong, Ikuhiko Kin and Gaku Mizuno
Number of samples: 82 adults and 19 juveniles
Locality: Southern side of Miura Memorial Library, Chubu University Campus, 1200 Matsumoto-cho, Kasugai, Aichi
Prefecture, Japan (35º16'N, 137º00'E).
Date: 26 November, 2018
Collectors: Seesamut Teerapong and Ikuhiko Kin
Number of samples: 26 adults and 17 juveniles
The northernmost recorded of M. phosphoreus in this study was in Akita Prefecture. The microhabitat in Akita University
campus is shown in Figures 1A-1B. The bright green bioluminescence when the earthworm was forcibly handled is shown inFigure 1C.
A total of 72 individuals of M. phosphoreus from the 3 sampling sites (Akita Prefecture, Miyagi Prefecture and Aichi
Prefecture) were counted in segments and measured in diameter and length (mm). Some specimens damaged to be examinedwere excluded. The length of 30 mm to 35 mm (N = 26) was the most abundant, follow by length of 25 mm to 30 mm (N =23) and only one sample was recorded for length less than 15 mm. The lengths of M. phosphoreus from all sampling sites werenormally distributed (Fig. 2). The relationships between number of segments, length and diameter of M. phosphoreus wereplotted in Figure 3A-3C, showing that the Akita specimens are large in diameter (1.49 ± 0.31 mm) compared with Miyagi(0.94 ± 0.12 mm) and Aichi populations (1.12 ± 0.16 mm). The relationship of the number of segments, length and diameter
showed low correlation between parameters. Adult M. phosphoreus (N = 72) had an average number of segment of 72.39 ±Figure 2. The frequency distribution of length of M. phosphoreus in this study (N = 72).

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S. TEERAPONG, I. KIN, P. SOMSAK, Y. OBA

Figure 3. (A) Number of Segments plotted against the diameter (mm) of M. phosphoreus
collected in 3 prefectures. (B) Length (mm) vs Diameter. (C) Number of segments vsLength. The plots of the specimen Mph91 and Mph92 (Akita population) were specified
in figures.

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Update on Northernmost Distribution of the Luminous Earthworm Microscolex phosphoreus (Dugès, 1837) in Japan

2.31. In the field surveys, potential predators were not observed during collection.
The COI sequences of 2 samples from Akita population had 100% nucleotide identity with M. phosphoreus according to theBLAST analysis. The nucleotide sequences were deposited at the GenBank under accession number LC455933 (sample ID,Mph91) and LC455934 (sample ID, Mph92).
DISCUSSION
Current record in Akita prefecture is the northernmost distribution of Microscolex phosphoreus in Honshu (Akita SakigakeShimpo, 2018), whereas no reported in Hokkaido and in this study we did not get M. phosphoreus in Hachinohe, AomoriPrefecture. The distribution of adult and juvenile earthworms across the 3 prefectures in Japan were examined. In total, matureearthworms were more abundant (78.18%) than non-mature. Akita prefecture had only adult specimens (100%), followed
by Miyagi prefecture (81.19%) and Aichi prefecture (60.47%). According to the geographical location, we suggest that theearthworm likely grows more rapid in northernmost part than southern parts in a cool temperate climate.
In this study, morphometric variations in M. phosphoreus were investigated. Total lengths in 3 localities varied from 14.86
mm to 37.61 mm, and number of segments from 68 to 77. The specimens of M. phosphoreus shows variation in number ofsegments and body dimensions, Dyne & Jamieson (2004) reviewed on the taxonomy of M. phosphoreus and described 10-55
mm in length with 73-91 segments, Taravera & Pérez (2009) reported 20-55 mm in length of M. phosphoreus in the western
Canary Islands. In the earliest description in morphological variation of this species in Japan, Yamaguchi (1935) examined
the Kanagawa specimens, showing 25-53 mm long (condition unknown) and 74-76 in segment number. In the most recently
reported by Rota et al. (2018) examined the Siberian specimens, showing 35-55 mm long (living condition) and up to 47 mm
after fixation with 73-79 segments. The lengths and segment numbers of M. phosphoreus investigated in this study are in therange of variations of the previous reports. A 658 base pair of the mitochondrial COI sequences from two Akita Prefecture’s
specimens showed 100% nucleotide identity to the M. phosphoreus of accession numbers LC108792 (Yamagata Prefecture,Japan; previous northernmost record), LC339499 (Fukushima Prefecture, Japan), AB750641 (Hyogo Prefecture, Japan),
LC198324 (Shimane Prefecture, Japan), and others. The molecular studies on the genetic diversity of the mitochondrial COI
gene have been reported in Japan by Oba et al. (2011a, 2011b, 2012, 2015, 2016) and worldwide scale by Rota et al. (2018),
and our present results showed the inclusion of the sequences from Akita Prefecture in the clade of the specimen from Japanand Australia, revealing no pattern in distribution. We found that Akita Prefecture’s specimens were larger in diameter butthe length and range of segment numbers were not different from other two populations. We chose the largest and one of thesmallest specimens in diameter from Akita Prefecture for DNA sequencing, but as shown above, there was not sequence
differences between them. It is still uncertain which factor is causative of the large in diameter of Akita specimens compared tothose of other two localities. In conclusion, this study reported the new record of the northernmost distribution of M. phosphoreus
in Japan in Akita Prefecture. It showed not only the highest percentage of mature earthworms but also the largest in diametercompared with other 2 sampling sites in southern regions. These results highlight for land use and management for themaintenance of this soil organism, which play dominant engineering roles in soil ecosystem.
ACKNOWLEDGMENTS
We would like to thank the Overseas Research Experience Scholarship for Graduate Student of Graduate School
Chulalongkorn University and Human Resource Development in Science Project (Science Achievement Scholarship ofThailand, SAST) for their support and encouragement. Authors thank Prof. Kenshu Fujiwara (Akita University) and Mr. Gaku

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S. TEERAPONG, I. KIN, P. SOMSAK, Y. OBA

Mizuno (Chubu University) for help collecting the specimens.
REFERENCES
Akita Sakigake Shimpo (2018) The first record of luminous earthworm in Akita Prefecture. Newspaper in 24th December,2018. (in Japanese) https://www.sakigake.jp/news/article/20181224AK0002/Dyne, G. R., Jamieson, B. G. M. (2004). Native earthworms of Australia II (Megascolecidae, Acanthodrilinae). ABRS,
Australian Government Department of Environment and Heritage. CD-ROM.
Folmer, O., Black, M., Hoeh, W., Lutz, R., Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochromec oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol., 3: 294-299.
Kumar, S., Stecher, G., Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets.
Mol. Biol. Evol., 33: 1870-1874.
Oba, Y. (2012) Record of the luminous earthworm, Microscolex phosphoreus (Dugès, 1837), from 14 points in Nagoya
University Higashiyama Campus. Bull. Nagoya Univ. Mus., 28: 77-83. (in Japanese)
Oba, Y., Branham, M. A., Fukatsu, T. (2011a) The terrestrial bioluminescent animals of Japan. Zool. Sci., 28: 771-789.
Oba, Y., Komiyama, R., Naito, M., Kin, I., Shibata, K. (2016) The northernmost distribution records of the luminous
earthworm Microscolex phosphoreus in Japan. Bull. Firefly Mus. Toyota Town, 8: 1-4. (in Japanese)
Oba, Y., Matsuda, M., Fujimori, N., Ikeya, H., Kawano, K. (2015) DNA barcoding of the luminous earthworm Pontodrilus
litoralis in Japan. Bull. Firefly Mus. Toyota Town, 7: 1-10. (in Japanese)
Oba, Y., Shibata, K., Yoshida, H. (2011b) The luminous earthworm, Microscolex phosphoreus (Dugès, 1837), found in Nagoya
University campus, and its DNA barcoding. Bull. Nagoya Univ. Mus., 27: 13-16. (in Japanese)
Rota, E., Martinsson, S., Erséus, C., Petushkov, V. N., Rodionova, N. S., Omodeo, P. (2018) Green light to an integrative viewof Microscolex phosphoreus (Dugès, 1837) (Annelida: Clitellata: Acanthodrilidae). Zootaxa, 4496: 175-189.
Talavera, J. A., Pérez, D. I. (2009) Occurrence of the genus Microscolex (Oligochaeta, Acanthodrilidae) at Western CanaryIslands. Bonn. Zool. Beitr., 56: 37-41.
Thompson, J. D., Higgins, D. G., Gibson, T. J. (1994) CLUSTALW: improving the sensitivity of progressive multiple
sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nuc. AcidsRes., 22: 4673-4680.
Yamaguchi, H. (1935) Occurrence of the luminous oligochæte Microscolex phosphoreus (Dug.) in Japan. Annot. Zool. Japon.,
15: 200-202.

6ホタルミミズの北限記録更新とその遺伝子および体サイズの解析
シーサムット・ティーラポン 1, 2, 3), 金 郁彦 3), パンハ・ソムサック 2), 大場裕一 3)
1)〒 10330 タイ王国バンコク チュラロンコン大学理学部生物科学プログラム

2)〒 10330 タイ王国バンコク チュラロンコン大学理学部生物学科動物体系学ユニット

3)〒 487-8501 愛知県春日井市松本町 1200 中部大学応用生物学部環境生物科学科

要約：発光性貧毛類ホタルミミズ Microscolex phosphoreus を秋田県秋田市（北緯 39º43'）から採集した．日本北限記録となるので報告する．同じ時期に，先の北限記録のひとつであった宮城県仙台市でも採集した．採集した個体は，秋田市産のものはすべて成体であったが，仙台市と春日井市（愛知県）のものは成体と幼体の両方が含まれていた．
キーワード：生物発光，北限記録，秋田県，ホタルミミズ

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