Gene Engineering Division
RIKEN BioResource Research Center

P‑1n RIKEN DNA BANK c:one name l pAxEFwtit2
R:=O Clone lD

RDB 05215

O Lot

19476

O DNA Concentration

25 nanogram/microliter

O Volume

40 microliter

O Form

DNA solution in TE burer

O HostDHS alpha

O Gulture

LB medium

O Antibiotics

100 microgram/ml Ampicillin

O Purification

QIAGEN QlAprep Spin Miniprep kit

BlJf

O Digestion by restriction enzyme
052■ 5ib)

ＨＯ Ｅ Ｘ

250 ngAvel!

Ｈ０３ ∽´コＯ Ｃ コ(NEB#N3200L),

Ｈ崎 寸 Ｑ ∽∪l kb P:us DNA Ladder

Lir$d. DNA-tlIlllU.
Obost(NEA#il30r21

■綺」
=‖麗―…

=

: 200 nanogram DNA per lane; 1o/o agarose gel , 1 x TAE Buffer
Expected size of fragment
Restriction enzyme
45.O
kbp
SwaI:Electrophoresis

Csp45=
XhoI

kbp

33.6′ ■0.0′ ■.52■ .2′ ■4.5′ 4.4′ 2.5′ ■.4′ 0.6′

0.4 kbp
kbp

O Confirmation of the insertion sequence
Please be sure to check our sequence analysis results before your request.
Sequence namePrimer lD

Primer name

Confirmed feature

Sequence - A

Pr0168Pr0084
PrPr347

EFla― CFEF‑l alpha pro,SV40 pA

pJB8-Forward

lTR.1,Ad5 psi,AxlT R!TR.2

Sequence - B
Sequence - C
Sequence - D
Sequence - E
Sequence - F
Sequence - G
Sequence - H

APPROVED BY
htlg:. / / dna.brc.ri ke n j pSノ

ADD:iedBibζ ystems

5:‐

11

AGTTAGGCCAGCTTGGCACTTGATG‑31241

131

21251

31

ACTT GT TTAT TG
151

351
361

371

41381

271

391

281′

171

401

291

181

‐CNCTNGC

631

411

301

I,NNT CNC.

191

641311￨2ρ l

Oct 26,202101:44PM,JSTOct 26,202101:59PM,JST

inst Mode:ノ Nanne 3500ノ3500 1nstrunnent

321101331

TG ACCCG211ヽTAATAATAAAACCCCAACTTTATCACTAACACAAAAT TATT CACATTT431441651

C T TNCGCC NC̲CTiCT■661451

5Ⅳ み1671,CGT CN CCCさ NCCCヽ N TTNC(CAA ATT TACTACAACA TCCGCC TAAAAC CC CCCGAAAATTGTCACTTCCTG TG TACI511521531541551621

111

P:ate NalTlei 2021 1 026 nlix′̲/ス

G AAT TAATT CATCG

Spacing:10.66 Pts′ Pane11 350

9■HiSQV Bases:661

Version 6.Oレレ

CCTCACACAG TG G TTCAAACTTTT TTTCTTCCAT TTCACGTGTC GTG
51617181161611

Electropherogram Data Page 1 of 2￨

Pts 1732 to 8663 Pkl Loc:1700

KB 3500 POP7 BDTv3.rnob05215 19476 BlJf 3 EFla― C F C02 08 .AB:2405215 19476 BlJf 3 匡Fla― C F

491

601

TCCCiC■ TCCGTCCCTTriCI.TG TGTTCC G CC

481591561

CA G C T T A T A A T G C T T A C A A A T A A A G C A A T A G C A T C A C AA A T T T C A C ト N N N N N G C A T T T T T T T CA C T G C A

5V字 。 ハ261

CTTATCATCTCTCCATCTCCAGGGCCCAACCTG TTTAAACCATGCTCACTア1N lN=N NNNlN T T T TTNAGTTT C CNNCTT G GTTlNT T

Cap:8NG:48A:45丁 :39C:36 primer nameハ : EFla― C̲F

11471581

CGT C C

461

ACT TCCTCTTATTCAGTTTTCCCGCG AAAATCCCCAAATCTTAC TC GGTTAC

571

C CG GCGC C CNN

on:Fri Jan 21,202202:22PM, JST

50

GCCGAAAACAC CT GAG AAAAACACCTGGG CG AGTCTCCACG TAAACG GTCAAAG TCCCCGCGCCCCTAC ACAAATATTAC G231

ヽGTTGTGGTTTGTCCAAACTCATCAATGTAT121

AAAT TATAAACTA GTCTAG AATCGATCNNN

KB l.4.1.8

KB.bcp

1334
667
01334
66701334
667
01334
667
01334
667
01334
667
Pri

N10:177A:107T:82C:79

又亦合B釧里ms
SノCap:1111121

1311´￨:3::pJB8‐ Forward

T TFAT TAA

TT覆′241

μ ♭ツ

581

361

251

591

261

601

HiSQV Bases:678

Pts 1715 to 8707 Pkl Loc:1683

Version 6.O05215̲19476̲BlJf̲3̲pJB8‐ Fon″ ard̲D02̲11̲A.B124
05215̲05215̲BlJf̲3̲pJB8‑Fon″ ard
KB 3500 P{Э P7 BDTv3.rnobt381

51

TTP O

271

161

391

281

171621

614()1291

181

631

71

411

301191

641

81421

541

2()1651

321

211

44ユ￨

A AI

111

561

GTCTTTTC

331

221

AGTGAC G TT TTT CGTCITGCC C

101

Plate Name:20211026 mix

Spacingi1 0.59 Pts/Pane11 350

Oct 26,202101:59PM,JSTOct 26,202101:44PM,JS丁

inst Modelノ Nanle 3500′ 3500:nstrunlent

91

431

TGATGAGTTTGGACA

6・ 71

Electropherogram Data Page 1 of 2

661

T,T TT CTAACCNT T■ TAACC TCCNATARA CAAGT TCNTCCA TTCTAC ANNNN NTNANA

531

ATTT13TCTACGGCCCCCCGGACTTTCACCGTTTACGTCGAGA̲CTCG CCCAGGTGTTTTTCTCAN311

CATCAATAATATACCT TATTTTCCATTCiACCCAATATCATAATCAG13GGGTCGAGTTTGTCACGTGCCCCG41151

AGTGTCCC CC AACTCTC AT GTT CCAACTGTIGGCCCAACACATGTAAGCCACCGATGTGGCAAA´7｀1.ら

ACGAGGCCCTTTCGTCTTC‐prlmer name 35'‐

NNNNNNTTClT R351」5Ψハ371

TGTACACACCAAGTGACAATTTTCGCGCGGTTTTAGGCGGATGTTGTACTAAATTTGGGCGTAA̲CCCAGTAACATTTCCCCATTTTCCCCGGAAAACTCAATAACACGAAG231571

CTAG AATGCAGTGAAAAAAAT CCTTTATTTGTCAAATTTGTCATCCTCGTTCCGGGTCAAAGTTGGC GTTTTATTATTATA GTCACCATCGTTTAAACA CCTTGGCC CCTCCAGATCCAC ACATC ATAA GATACAT'461471481491
501511521341

TGAAATCTGAATAATTTTGTGTTACTCATAGCGCGTAACC

GGCCGTGGGAiCCCGGCGGCTCACGTA

1

Nさ NN NlN NNヽ N

KB.bcP
KB l.4.1.8

1054
52701054
52701054
52701054
52701054
52701054527
Printed on:FriJan 07,202210:43AM,JST郎
島螺

Cap:24ms

S/NG:31A:24丁 :23C:24
KB.bcp1111O
τY仄 しト351

primler narne̲.̲⌒ ⌒ ̲ ^^C・̲̲:AxIT̲R1413a切

11‐05215 19476 BlJf 3 Ax!丁 R H03 24 AB!24R05215 19476 BlJf 3 A)《KB̲3500̲POP7̲BDTv3.mobP)ts 1817 to 8707 Pkl Loci1785

Version 6.O HiSQV Bases:674

151

41381

271

161

51

391

281

171

61

401

291

181

71

411

301

191

81

■11321

551

CA■

211

441

101

331

561

471

i■481

601

491

611

501

621

戸OPll たェ 521↑,′ ・)a)に +631

ATC T AACA■ T GCGCTC■ T CGT CATCCTCCCCN ICG TCACCC TCC NNGCTCTACGC
591

ホA.531671

1111

TGC CGa6811￨Electropherogram Data Page 1 of 2TN NNCNTNN N Tさ NC CNNGT NCN

651451

CCiCTGGAAGCACTGT221GATCTCCGdSpacing:10.71 Ptsノ Pane:1350
Plate NalTle:20211022 nlix

Oct 22,202104:26PM,JSTOct 22,202104:41PM,JST

inst Mode:ノ Name 3500ノ 3500 1nstrunnent

91■TR0431≦とs̲⊆ ≦とQAAcAcccTccTCAT

ハ,a.1421541.CATTTTCCCCACCTGTGTAGGTTCCAAAATATCTAGTIGTTTTCATTTTTACTTGG'TCAIGC AACC CA GCA CT

1261xし こ1‖371

201

ATC GT CACTT CCGTTTTCCCACGTTACGTCACTTCCCATTTTAA(GAAAAcTAcAAT T CCCAA cACATA CAA GTTA

5'丁 CCACAAAAAACACCCAGAA3'131251

AAA TC GAACATG C CG ATCCIL⊆ …ェ」二⊆̲▲ GTCAACAG CACAAACATACAAGCTGTCAGCTTTGCACiÀユ361

AT GTGCAAAACACC ACGC■241

TAC(3TCACCCGCCCCGTTCCCACGCCCCGCCCCACGTCACAAACTCCACCCCCTCATTATCATATTGGCTTCAATCCAAAATAACGTATATTATTCAT21

NNNNさ NNN〕 NN NlNNNN NN CTT C■231

CGAATTAATTAAATC G,CCTAAAAC

1NさKB l.4.1.811565780115657801461

3A GTCACGCACCGTG TAT571581641ATAAGCTTTATCCTTATCCAAAACAGCCTTGTGGTCAGTGlTCATCTGCTGACTGTCGACAISISSGC!TGCA3JIIITAAlGCGGTAGTTTlTCACAGTTAAATTGCTAACG

341]GTTGCTGTGTTAGT,■′し う'′ 9̀｀1156
57801156
57801156
57801156
5780
Printed on:Fri Jan 07,202210:43AM,JST