2007 Meeting of theSociety for
Whole-Body Autoradiography
March 18-21, 2007
Mill’s House Hotel,
115 Meeting StreetCharleston, South Carolina, 29401,
USAMEETING AGENDA
MONDAY MARCH 19, 2007

Registration & Continental Breakfast
Welcome9:00 am to 9:15 am

8:00 am to 9:00 am

Eric Solon, SWBA President

KEYNOTE SPEAKER 9:15 am to 10:15 am

Dr. David Wilson

Professor of Biomedical Engineering and Radiology
Case Western Reserve University
Founder BioInVision, Inc.
Session ITime

Session Chair

Introduction to Session

10:15 am to10:20 am
10:20 am to10:50 am

William Waddel, University of Louisville, US

Speaker IAMorning Break & Posters
Speaker IB
Session SummaryGroup Photo
Meeting Luncheon
Session II

Introduction to Session
Speaker IIA
Speaker IIB

11:20 am to
11:50 am
11:50 am to
12:00 noon

Alfred Lordi, Quest Whole-Body Tissue Distribution
Pharmaceuticaland Metabolic Profiling of 14CServices, US
AZT in Fetal and Maternal Tissues
10:50 am to 11:20 am
Eric Solon, Quest
Distribution of [14C] Dalbavancin
Pharmaceuticalin Rabbit Bone and Related Tissues
Services, US
William Waddel, University of Louisville, USTime

Session Chair

2:00 pm to2:30 pm

Alain Schweitzer,
Novartis,
SwitzerlandMarissa Vavrek,Merck, US

1:25 pm to1:30 pm
1:30 pm to2:00 pm

Afternoon Break & Posters
Speaker IID
3:00 pm to3:30 pm
Speaker IID

3:30 pm to4:00 pm

Session Summary

4:00 pm to
4:05 pm

Drug Discovery andDevelopment

12:00-12:15pm
12:15-1:25pm
Drug Discovery & New Methods

Brian Whitby, Covance Labs, UK

Macro-Autoradioluminography ofHard Tissues

Metabolite ID from QWBA
Sections Using Advion Nanomate
ESI MS
2:30 pm to 3:00 pm
David Wilson,
Case Western
Whole Mouse Cryo-Imaging
Reserve University,USMichael Potchoiba, A Quantification Method For
Pfizer, Groton, Ct,
Determining the Biodistribution ofUSA
Tritium Labeled Compounds Using
WBAL
Brian Whitby, Covance Labs, UK

Pre-Dinner Cocktails (Bus to Magnolia Plantation @ 5pm)
Conference Dinner(Honors to be Bestowed)

6:00 pm to 7:00 pm
7:00 pm to 9:00 pm

MEETING AGENDA

Continental Breakfast

TUESDAY MARCH 20, 2007

8:00 am to 9:00 am

Session III

TimeSession Chair

Introduction to Session

9:00 am to
9:05 am
9:05 am to
9:35 am

Alfred Lordi, Quest Pharmaceutical Services, US

9:35 am to
10:05 amKen Koeplinger,Merck, US

Speaker IIIC

10:30 am to11:15 amSpeaker IIID

11:15 am11:45 am
11:45 am to
11:50 am

Tadafumi
Glass Slide Transfer of WholeKawamoto, Tsurumi Body Section for Histology
University, Japan
Examination
Helen Minter,
Skin Penetration and MicroUnilever, UK
Autoradiography
Alfred Lordi, Quest Pharmaceutical Services, US

Speaker IIIA
Speaker IIIB

Morning Break & Posters

Session Summary

Meeting Luncheon

Alain Schweitzer,
Novartis

Instrumentation &New Applications

Label-free Molecular Imaging ofWhole-body Tissues Sections byMass Spectrometry
QWBA vs 19F Magnetic
Resonance Spectroscopy (MRS)
for a Fluorinated Nucleoside
Drug: Translation of PreclinicalTissue Distribution Studies tothe Clinic

10:05 am to 10:30 am

12:00 noon to 1:00 pm

Session IVTime

Session Chair

Introduction to Session

1:00 pm to
1:10 pm
1:10 pm to
1:45 pm

Alain Schweitzer, Novartis, Switzerland

Speaker IVA

Speaker IVB

1:45 pm to
2:15 pm

Cinthia Pastukova,
Genentech, CA,
USA
Andrew Davis,
Cameca, Trumbull,
CT, USA

Drug Discovery & New
Methods
The effect of immune-complex
formation on the biodistributionof [125I] labeled antibody in amouse model: The use ofQWBA in drug discovery
NanoSIMS – Subcellular Isotope
Tracer Imaging

Poster & Exhibit Viewing & Afternoon Break
2:15 pm to 3:00 pm
3:00 pm to Chairman: Alain Schweitzer, Novartis,
ISSUES IN
5:00 pm
Switzerland
PHARMACEUTICAL
Panel of Session Chairs
RESEARCH - OPEN
FORUM
(Dinner is up to individual)MEETING AGENDA

Continental Breakfast

WEDNESDAY, MARCH 21, 2005
8:00 am to 9:00 am

Session VTime

Session Chair

Introduction to Session

9:00 am to
9:05 am
9:05 am to
9:35 am

Eric Solon, Quest Pharmaceutical Services, US

9:35 am to10:05 am

Birte Hofmann,
Bayer HealthCareAG, Germany

Speaker VA

Speaker VB

Morning Break
Speaker VC

10:30 am to
11:00 amSpeaker VD

11:00 am to
11:30 am

Summary of Session

11:30 am to
11:45 amMeeting Close
Eric Solon

Brian Whitby,
Covance UK

Regulatory & New
Applications continued

The practicalities of carrying out
ADME studies using large
molecular weight radiolabelled
bio-molecules
Answering Specific Questions
Preclinical Questions usingWBA

10:05 am to 10:30 am

Lee Crossman,
Schering-Plough,Kenilworth, NJ,
USA

Distribution of 14C-FlorfenicolDerived Radioactivity to
Therapeutically-TargetedTissues in a Calf Administered aSingle Subcutaneous 14CFlorfenicol Dose as Resflor
NMP (SCH 529752).
Alain Schweitzer,
Relevance of the Section
Novartis, Basle,Thickness Uniformity andSwitzerland
Section-to-Section Thickness
Reproducibility for
Quantification
Eric Solon, Quest Pharmaceutical Services, US

11:45 am to 12:00 noonPreparation of Multi-Purpose Fresh-Frozen Sections By Using a New Adhesive Film
Tadafumi Kawamoto, Radioisotope Research Institute, Tsurumi University, School of Dental Medicine,
2-1-3 Tsurumi, Tsurumi-Ku, Yokohama, Japan 230-8501; e-mail address: kawamoto-t@tsurumi-u.ac.jp
I reported on a new approach to preparing thin whole-body sections for histochemistry,
immunohistochemistry, and light microscopical autoradiography at this conference in 2001. Cutting
procedures were nearly identical to those used in the Ullberg method. However, to better prepare highquality sections, a new adhesive film and disposable blade were used. This method resulted in 2 µm thickfrozen sections that could be examined at high magnification with a light microscope. However, themethod is not suitable for routine work because the adhesive film must be prepared before each use.
In 2003, I created following adhesive film sheets for routine work. Each film sheet is supported byreleased paper. Each sheet is cut to match the size of a sample block surface.
1. Cryofilm Type-1 has the strongest adhesive power. It supports a frozen section at approximately 29°C. The section can be immersed in acetone. Sections supported with Cryofilm Type-1 are usedfor histological staining without toluidine blue, histochemical staining, immunohistochemical
staining, and in situ hybridization. The stained section is preserved between the adhesive film andthe glass slide.
2. Cryofilm Type-2 has less adhesive power than Cryofilm Type-1. It does not permit dipping in either
acetone or xylene. However, it allows histological staining with toluidine blue as well as
histochemical staining, immunohistochemical staining, and in situ hybridization.
3. Transfer film is also used for histological staining, histochemical staining, immunohistochemical
staining, and in situ hybridization. However, direct transfer of the stained section from the adhesivefilm to a saline-coated glass slide is possible.
4. LMD film is used to support sections cut by laser microdissection techniques. Collected specimens
are used for gene analysis.
I have tried to develop adhesive films usable with a wide range of samples. I have devoted considerable
attention to embedding mediums and mounting mediums. Design improvements in disposable blade
holders and sample holders have been made. Cryofilm Type-2C is my most recent effort. It is used tosupport frozen sections at -37°C. It can be immersed in organic solutions such as alcohol, acetone, andxylene. Sections can be placed in a hot chamber (125°C) for more than one hour. Cryofilm Type-2Cpermits many types of staining including toluidine blue. The film adheres to a frozen block made of a
new embedding medium strongly. This is an improvement over conventional embedding mediums suchas CMC gel or OCT. Section preparation, staining, and mounting procedures are nearly identical tothose described at this conference in 2001. The frozen block is cut with a disposable tungsten carbide
blade (Leica TC65). Exposed tissue surface is covered with the adhesive film and then cut into 2∼40 µm
thick sections. The sections are removed from the cryochamber and instantaneously thawed. They arethen immersed in 100% ethanol, fixed with a 4% paraformaldehyde solution (pH 7.4), and stained. The
stained sections are sandwiched between the supporting plastic film and the glass slide for permanent
preservation. Of course, the new embedding medium and mounting medium are used.
Thin sections from a rabbit thighbone, human bones, and other large samples (adult mice and rats)
have been made. Complete thin sections (2 µm) have been prepared from adult rat thighbones, soft
tissue, and a whole baby rat. Frozen sections from many other samples are possible. These include
plants, fish, insect, grains, and assorted foodstuffs. Sections are applicable to a wide range of researchtechniques including histological staining, enzyme histochemistry, immunohistochemistry, in situ
hybridization, laser microdissection, electron probe microanalysis, and autoradiography of water-soluble
materials. Indeed, it is a very versatile and useful tool for a wide spectrum of biological research.
At this conference, I will use video and slide presentations to describe my methods and theirapplications.

MEETING ATTENDEES
Last NameFirst Name
Company nameAnciaux
Katelijne
Janssen Pharmaceutica
Brown
Richard
Lablogic Systems, Ltd.
Crossman
Lee
Schering-Plough Research Institute
Dorenkamp
Claudia
Leica Microsystems Nussloch GmbH
Erickson
Jamie
Abbott Bioresearch Center
Flood
DennnisNovartis Pharmaceuticals
FultonJeffrey
Hoffmann-LaRoche, Inc.
GeertsRita
Janssen Pharmaceutica
Hofmann
Birte
Schering AG
Kawamoto
Tadafumi
Tsurumi University
Knapp
Brian
Covance USA
Koeplinger
Ken
Merck Research Labs
KorsenAnn
Leica Microsystems, Inc.
LaRocca
Shawn
Leica Microsystems, Inc.
Linehan
StefanWIL Research Labs, LLCLordi
AlfredQuest Pharmaceutical Services
Marlowe*Carolyn
William J. Waddell, Inc.
McKownJon
biospace USA
McNally*
William
SWBA Distinguished Fellow
Mehta
Drew
Vibratome Company
MinterHelen
Unilever
Partridge
Elizabeth Ann AstraZeneca
Pastukovas
Cinthia
Genetech Inc.
Patterson
AndrewCharles River Laboratories, UK
Potchoiba
MichaelPfizerPress
Randy
Covance USA
Rebmann
Nina
QPS
Ren
Xiao
sanofi-aventis
Schweitzer* Alain
Novartis Pharma
Sved
Dan
WIL Research Labs, LLC
Solon
Eric
Quest Pharmaceutical Services
Trawick
Dorothy
Array BioPharma
VavrekMarissa
Merck Research Labs
Viot
Delphine
UCB (Belgium)
VollerThomasWashington University School of Medicine
Wadell*Bill
University of Louisville
WhitbyBrian
Covance Labs Ltd.
WilsonDavid
Case Western Reserve Uinversity
Zhen
Ji
sanofi-aventis
ZimmerManfredsanofi-aventis• Distinguished Fellow of the Society for Whole-Body Autoradiography