Gene Engineering Division
RIKEN BioResource Research CenterF)… 1n RIKEN DNA BANK cione name:pAxCAwtit2R!r=関O Glone lD

RDB 05213

O Lot13981

O DNA Concentration

25 nanogram/microliter

O Volume40 microliter

O Form

DNA solution in TE burer

O HostDH5 a:pha;;

O Culture
O Antibiotics

BlLr

LB medium
100 microgram/ml Ampicillin

QIAGEN Q:Aprep Spin Miniprep kit

O Purification

O Digestion by restriction enzyme{kb)

Ｈ〇 二 Ｘ

250 ngノwel:

Ｈ口３ ∽´コ υ ⊆ ⊃(NEB#N3200L),

３ Ｈｎ寸Ｑ∽０・05 2l kb Plus DNA LadderLrnb& DNA{IIEUIt)ig€€t6.0一一︼︼『″￢L二 」

G{EB*t(I)r2}

U盤 田壼bhu■―"": 200 nanogram DNA per lane ; 1% agarose gel , 1 x TAE Buffer
Restriction enzyme
Expected size of fragment

Electrophoresis

45。 0SwaI
Csp45=
Xho=

33.6′ ■0.0′ ■.52■ .2′ ■4.5′ 4。 8′ 2.5′ ■.4′ 0.6

kbp
kbp
kbp
kbp

O Confirmation of the insertion sequence
Please be sure to check our sequence analysis results before your request.
Sequence namePrimer!DPrimer name

Confirmed featureSequence - A

Pr0584Pr0008PrPr347

CAG RlAmp̲RAxl丁 R

CAG pro,beta-globin pA

Sequence - B
Sequence - CAmpR prolTR.1,Ad5 psil丁R.2

Sequence - D
Sequence - E
Sequence - F
Sequence - G
Sequence - H

APPROVED BY
htlp: / / dna.brc. ri ken j p

N(3:88A:50T:59C:62

バB金 8醐里ms
SノKB.bcp
Cap:2461

351

111―一:一→cハ q Prク

31

251

141

261

151

271

361

｀481

371

601

381

Jan ll,202202:23PM,JS丁Jan ll,202202:08PM,JST!nst Mode!ノ Name 3500ノ 3500:nstrument

KB 3500 POP7 BDTv3.lTlob

Spacing:10.67 Ptsノ Pane11 350

501

391

171511

401

61291

181

411

521

6」 171191

421

531

641

C T TGCC NNT T CCT GGCTCNNNT G(b- glob| *621

C

81

201

431

91

5ZL

211

X♭ 4「101221

CC TCTCCTAACCATG

331

3411

lE c(k L
9′ ιr X♭ ′451

561

TCAI

TCGGAAGGACATAT

671

Electropherogram Data Page 1 of 2661

G ̲

551

441

S″ 久1

ATCttTTTAAAT TATAAActtGTCTAG

JLr/ιlaI651

C GNT GG CT TN

541

A PI,TAAAC GAAATT TATTTTCAT TCCAATAGTGTGTT CGAATTTTTTGT GTCTCTCA(-611ＺΠ

591′︱

581111

Pts 1719 to 8704 1Pkl Loc:1687

161281301

GCGGGG,GGCCCTTCGTCCGTCGCCCCCCCGCCGTCCCCTTCTCC

Plate Nanne:2022011l dnabank

Version 6.O
51

CGCCC C CiGGβACGAA■ TCG

HiSQV Bases:690

05213 13981 BlLr l CAG Rl

05213̲13981 BlL「 l CAG Rl B01 02 AB!08°tι r

41

G CG GGCGCC GGG CG AAGCG GTCCG G

Primer namc.ハ:CAG̲RllTTGTCCCAAATC′「 GTGCGGA‑3'5'―131GTCCCCGGGGGGrlCGGCTGCCTTCGGGGGGCtCCCGGCiCGGCGGGGTTCGGCTTCTCCCGTGTCiCCGGCGG

NNNNC T241471

TG GGGACATCAT CAACCCCC TTGAGCATCTCACTTCT CCC571

GG G AC GCCAAATCF、 TT TAAAACAT CAIGAATCAGTATTTCIGTTTACA GTTT C GCA491

GATAATCAATTCACTCCTCAGGTGCACCCTGCCTATCACAAGGTCGTGGCTGGTCTGGCCAATGCCCTGGCTCACAAATACCACTGAGATCTTTTTCCcTcTGCCAAAAATTA231

TTCAT GCCTTCTTCTTTTTCCTACAGCTCCTCGGCiACGTGCTG GTTATTGTCCTGTCTCATCATTTTGGCAAAGA3TTGATTT121

CTCTCCACCCTCGGGCCT1NNヽ さNNNNN NNN Nヽ

KB l.4.1.8114057001140570011405700114057001140570011405700

Printed on:Tue Jan ll,202202:52PM,JS丁ittms
金8醐
1Capi35'―l121ぅ:Amp̲R

3105213̲139霜;IF̲軍 11:三棚ポ

KB̲3500̲POP7̲BDTv3.mob

<I

PB!08

frrnY\7*

CC(〕 ACACGGAAATGTTGAATA‐3,Pts 1708to 8704 Pkl Loc:1676
versiOn 6.O HiSQV Basesi 670
GGTTATTGTCTCNTCAGN
ATAT TT C AAT GTAT TTA CAAAA■
4151
16171

S/NG:94A:77丁 :70C:531)rirncr namC

l231

121

3
1461

571

131

581

471

GT GTTACT CATAIGC CCGTAA

141371261151271

161lT卜 (D381491TGTCTACCGCCGCCGGG
601

481

591

■ TT

391

501

611

281

171

401

511

621

291

181

411

521

631

301

3112

ゝ221

111

CACATTTCCCCG A101

̲ム

331

1- AJT

551

61

̀

′671

GGG

561

T:ilACACAGGA]」´￨′ ι ̲'̲

211.

P!ate Nannei 2022011l dnabank

Spacingi1 0.81 Pts/Pane11 350

Jan ll,202202:23PM,JSTJan ll,202202:08PM,JS丁

inst Mode!ノ Narne 3500/3500!nstrument「431541651

Electropherogram Data Page 1 oI 2

321

TAAACAAATA C GG GTTCCCC
81
91

191

421

531

641

TTTG■ NCGTTTACGTGGiCNCTCGCCCANGTCNTTTTCTCNNGTGTTTTCC￨ヽATCATCAATAATATACC TTATTTTGGATTCAAGCCAATATCATAAT CACG GGGTCGAGTTTGTCACGTC CC(3CGGGG CGTCCCAACG GG
251

4← r

361

AGTGTG GCGGAAGTGTCATGTTGCAAGTGTGGCC CAACACATGTAAGCCACGG ATGTCCCAAAAGTCACG TTTTTGGTGTGCCCCG

侮CrAT TTCCATTTAATTAAヽTTTT〕TCACAATTTTCCCGCCGTTTTAGGCGGATGTTGTAGTAAATTTCCCCGTAACCCAGTAACATTTCCCCATTTTCGCGGGAAAACTGAATAAGAGGAAGTCAAATCTCAATA

341

CGGGTGACG TA241

AAAGTCCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATACGCGTATCACCAGGCCCTTTCGTCTTCAAGAATTCCGGiTCGATCCCCATGTTCG%α V

NN NNNNNヽ NNさ NNNN N N N NNNNNT TT,.TCN G

KB l.4.18

KB.bcp12026010120260101202
60101202
60101202
60101202
601
Printed oni Tue Jan ll,202202:52PM,JSTPrimer name (,:AxlT_Ri″

雨 1151″ヽlTR t[ソ371

261

491

381

Jan ll,202202:08PM,JSTJan ll,202202:23PM,JST:nst Model/Nanle 3500/3500 1nstrunnent

91

281

61

401

291

71

411

631

301

81

531

641

311

431

541

651

221

441

551

661

TC CTCG GCAC CG TCACC CTGINAN CCT GTAN GCAT AN C CT NN

521

101

561

111

Spacingi10.69 Pts/Pane11 350

391

511

621

211

P:ate Nanne1 2022011l dnabank

271

501

611

Electropherogram Data Page 1 of 2331

HiSQV Bases:688

Version 6.O51ｌＶ＝１
Ｈ ＝Ｖ:l361481601321

Pts 1673 to 8704 1Pkl Loc:164105213 13981 BlLr l AxlT R D01 04 .AB10805213 13981 BlLr l AxlT R
KB 3500 POP7 BDTv3.ITlob

41

CAACTTCCTCAAATCGTCACTTCCGTTTTCCCACG TTACGTCACTTCCCATTTTAAGAAAAcTAcAATTCCCAACACA

5' - TCCACAAAAAACACCCAGAA- 3'FI351471591421d T̲ECGAATTAAT TAAAT CGAAATCCAACAT CC GGATCCTC TACAGTCAACACCACAAACATACAACCTGTCACCTTTGCACAAGGCCCCAACACCCTG CT

41

TAAAACCTACGTCACCCCCCCCGT TCCCACC CCCCCCGCCACG TCACAAACTCCACCCCCTCATTATCATATT CCCTTCAATCCAAAATAAGGTAT131141151
161
171
181
191
2o131 1

Cap:4

NG:95A:76丁 :78C:87

バBttB馴見msSノKB.bcp

121

21

NN NN NNNNNNヽ NNllNNNlNさ N NNN

231

TTAT TCATCAT341

461

581

̀al多CCACCACTCCACTCGATAACCATTATCCTTATCCAAAACACCCTTGTGGTCAGTGTTCATCTGCTCACTGTCCACCTCCAGCCATGCAACCTTTAATCCCGTAGTTTATC571

CA GT TAAAT TGCTAACGC AGTCAC CCACCGTGTATTAAATCTAA CAA TCC CC TCATC GT C■

1TCAAGAAGCACTGTGGTTCCTGTGTTAGTAATGTCCAAAACACGAGGCACATTTTCCCCACCTGTGTAGGTTCCAAAATATCTAGTGTTTTCATTTTTACTTCCATCAGGAAくつACAAGTTACTCCGCl

ささN

KB l.4.1.8

1164
58201164
582011658201164
58201164
5821164
582
Printed oni Tue Jan ll,202202:52PM,JS丁