TARGETED METABOLOMICS ANALYSISAT YOUR FINGERTIPS

Agilent Metabolomics dMRM Database and Method
Routine analysis of central carbon pathway metabolites
Targeted metabolomics experiments based on Triple Quadrupole LC/MS
can be used to identify altered reactions and disrupted pathways within adefined group of metabolites in a cell, tissue or organism. Depending on thesize of the target metabolite group, method development can be very time
consuming and requires availability of a wide range of standards to optimize
MRM transitions and retention times.
The metabolomics dynamic MRM database and method for >215
metabolites in the central carbon pathway enables straightforward
implementation of a targeted metabolomic method in your research lab.
Relying on Agilent’s 1290 Infinity II Series LC and 6400 series LC/MS
systems, the ion-pairing LC/MS method enables effective separation of key
compounds with stable retention times. The compound database with MRM
transitions was developed and curated together with the Rosebrock lab, aleading lab in the metabolomics field, for generating highest quality results.

The Metabolomics dMRM
Database and Methodincludes:• Curated database with >215
compounds from central carbon
metabolism pathways• Optimized MRM transitions for allcompounds in the database• Retention times for all compounds
in the database• Quick-start guide with example data
and familiarization exercises• Method setup guide with arecommended LC/MS acquisition
method• Technical note with typical results

Learn more
www.agilent.com/chem/
dmrm

Glycolysis Pathway

Straightforward method implementation• Curated database eliminates the need to develop MRM transitions for alarge numbers of compounds.

Complete workflow solutions
from Agilent• Method guidance enables quick implementation of a dMRM screening
method for all compounds in the database.
• Robust ion-pairing method provides superior separation and stableretention times.

00

51015

Average retention time (minutes) across 62 samples

2020
1015

Agilent MassHunter data acquisition
and analysis software lets you
quickly implement targeted screening
methods, which you can modify tomeet your future needs.

551015Retention time for 62 individual sample injections

20

Retention time after >4000 injections (seven months of use)

B.

0A.

051015Retention time (minutes) for fresh column

20

Retention times (RTs) are very stable with this method. A. RT versus average RT for 62 different yeast extracts
analyzed over 3 days B. RTs in yeast extract analyzed before and after 4000 injections of biological samples.

Extensive pathway coverage using targeted analysis
The dMRM database and method are part of Agilent’s comprehensive
metabolomics workflows and results are supported in Agilent’s leading
software workflow including Mass Profiler Professional and Pathway
Architect. Compounds in the database include a wide range of compoundclasses from central carbon metabolism, including organic acids, sugars,
sugar phosphates, and nucleotides.

Database curation produces high quality results

The Agilent 1290 Infinity II LC
system and Agilent 6400 Series
Triple Quadrupole LC/MS systems
are proven choices for quantitative
applications, giving you unmatchedseparation performance, superiorsensitivity, renowned reliability, andoverall system robustness.

Each curated database entry includes:• Compound common name
• MRM transitions (precursor and product m/z)
• Fragmentor voltage• Collision energy• Retention time and retention time window• Unit mass of neutral molecule• Molecular formula• CAS number of the native compound

Best-in-class application consulting
and a large portfolio of sample
preparation products, LC column
phases, and other consumables
round off Agilent’s complete
workflow solution.

Ordering information
Product Description

Part Number

Agilent Metabolomics dMRM Database and Method• Database with >215 compounds from the central carbon pathway• Optimized MRM transitions for >215 compounds• Retention times for >215 compounds• Screening methods for analysis of all compounds in database• Quick Start Guide

G6412AA• Method Setup Guide• Analysis guide with sample preparation information• Database content list
• Related application and technical notes

Recommended Instrument Configuration and Consumables
The following are required but not included
with the database:Product Description

Part Number

1290 Infinity II Flexible Pump

G7104A

1290 Infinity II Multisampler

G7167B• Agilent 1290 Infinity II LC system –see Table for configuration listing
major compounds

Agilent Infinity Sample Cooler

G7167B #100

• Agilent 6460/6470 Series Triple
Quadrupole LC/MS system

1290 Infinity II Multicolumn Thermostat

G7116B• Agilent MassHunter acquisition software
B.06 or higher and Windows 7 64-bit

Valve drive for 1290MCT

G7116B #058

• Agilent MassHunter quantitative analysis
software B.07 or higher

2 pos/6 port ultra high pressure (1200 bar) valve head

5067-4117

ZORBAX RRHD Extend-C18, 2.1 x 150 mm, 1.8 µm

759700-902

UHPLC Grd, Extend-C18,2.1 mm, 1.8 µm, 3pk

821725-907

To learn more about Agilent
metabolomics tools, call
800-227-9770 (in the United Statesor Canada) or visit
www.agilent.com/chem/dmrm
For Research Use Only.
Not for use in diagnostic procedures.
Information is subject to change without notice.
© Agilent Technologies, Inc. 2016
Published in USA, July 19, 2016
5991-6467EN