ZEB1 Antibody
Rabbit Polyclonal
Antigen Affinity Purified

Protein ID

NP_110378.3

Catalog No.

A301-921A-T

GeneID

6935

Lot No.

A301-921A-T-2

APPLICATIONSWB, IP

SPECIES REACTIVITY

Human

AMOUNT

10 µl

CONCENTRATION

200 µg/ml

STORAGE/SHELF LIFE

2 - 8°C / 1 year from date of receipt

PHYSICAL STATE

LiquidBUFFER

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide

ISOTYPE

IgG

ORIGINUSA

PRODUCTIONPROCEDURES

Antibody was affinity purified using an epitope specific to ZEB1 immobilized on solid
support.
The epitope recognized by A301-921A-T maps to a region between residue 850 and 900 of
human zinc finger E-box binding homeobox 1 using the numbering given in entry
NP_110378.3 (GeneID 6935).

APPLICATIONSADDITIONAL INFO

Centrifuge tube to remove product from lid. Optimal working dilutions should be determined
experimentally by the investigator. Prepare working dilution immediately before use.
Western Blot

1:2,000 - 1:10,000

Immunoprecipitation

2 - 5 µg/mg lysate

https://www.bethyl.com/product/A301-921A-T
Use the link above to view SDS, a current list of citations, and other product specific information.
IP-western blot protocol: https://www.bethyl.com/content/protocol_IP_WB

This document certifies that this product has met all of the quality control standards defined by Bethyl Laboratories, Inc.
Michael Spencer, PhD

Date: June 6, 2022

Phone: 800.338.9579 • Fax: 866.597.6105 • Web: www.bethyl.com
Orders: orders@fortislife.com • Support: technical@fortislife.com

1

For in vitro laboratory use only. Not for any clinical, therapeutic or diagnostic use in humans or animals. Not for human or animal consumption. This product may not be resold or modified for resale without the prior written
approval of Bethyl Laboratories, Inc. The information provided in this data sheet is believed to be correct but does not purport to be all-inclusive and is intended to be used as a guide. Bethyl Laboratories, Inc. shall not beliable or responsible in any way for use of either this information or the material supplied. Disposal of hazardous material may be subject to federal, state or local laws or regulations.

ZEB1 Antibody

A301-921A-T

Detection of human ZEB1 by western blot. Samples: Wholecell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells
prepared using NETN lysis buffer. Antibody: Affinity
purified rabbit anti-ZEB1 antibody A301-921A (lotA301-921A-2) used for WB at 0.1 µg/ml. Detection:
Chemiluminescence with an exposure time of 3 minutes.

Detection of human ZEB1 by western blot ofimmunoprecipitates. Samples: Whole cell lysate (1.0 mg
per IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Affinity purified rabbit
anti-ZEB1 antibody A301-921A (lot A301-921A-2) usedfor IP at 3 µg per reaction. ZEB1 was also
immunoprecipitated by rabbit anti-ZEB1 antibody
A301-9221A. For blotting immunoprecipitated ZEB1,A301-921A was used at 1 µg/ml. Detection:
Chemiluminescence with an exposure time of 30 seconds.

Phone: 800.338.9579 • Fax: 866.597.6105 • Web: www.bethyl.com
Orders: orders@fortislife.com • Support: technical@fortislife.com

2

For in vitro laboratory use only. Not for any clinical, therapeutic or diagnostic use in humans or animals. Not for human or animal consumption. This product may not be resold or modified for resale without the prior written
approval of Bethyl Laboratories, Inc. The information provided in this data sheet is believed to be correct but does not purport to be all-inclusive and is intended to be used as a guide. Bethyl Laboratories, Inc. shall not beliable or responsible in any way for use of either this information or the material supplied. Disposal of hazardous material may be subject to federal, state or local laws or regulations.