Other issues to be urgently addressed
Title of Research:
13_PT05-01-3

Development and practical verification of a novel comprehensive
monitoring system for multiple contaminations of environmental
pollutants in the Mekong River basin
Principal Investigator:
Kazumasa Hirata, PhD (Professor, Osaka University, Pharmaceutical Sciences; 1-6 Yamadaoka,Suita, Osaka 565-0871, Japan)(tel) +81-06-6879-8236, (e-mail) hirata@phs.osaka-u.ac.jp
Collaborators:
Kazuo Harada, PhD (Osaka University, Pharmaceutical Sciences); Hideyuki Matsuura, PhD(Osaka University, Pharmaceutical Sciences); Yoshinori Sumimura (Osaka University, Global
Collaboration Center); Nguyen Quang Trung (Institute of Environmental Technology, Departmentof Environmental Toxic Analysis); Misa Muraoka (Osaka University, Pharmaceutical Sciences);
Tran Thi My Duyen (Can Tho University, College of Aquaculture and Fisheries); Jun-ichi
Nishikawa, PhD (Mukogawa Women’s University, School of Pharmacy and PharmaceuticalSciences); Taku Yamashita (Mukogawa Women’s University, School of Pharmacy andPharmaceutical Sciences)
Summary of Research:
Serious environmental pollution of the international river basins of Southeast Asia, such as theMekong River basin, has been caused by multiple contaminations of pollutants such as
agricultural chemicals, endocrine disruptors, heavy metals, and antibiotics. It is, therefore,
important to monitor food and environmental samples from these areas to ensure public safety.
The aim of our project is to develop a novel comprehensive monitoring system for multiple
contaminations, for use in ASEAN countries. In the present study, we mainly focused on
constructing biosensors for sulfonamides that inhibit bacterial folate synthesis and other antibiotics
that inhibit bacterial protein synthesis. Each antibiotic was detected using a series of enzymatic
reactions performed on pattern-printed papers, such as those including a folate synthetic enzyme
or in vitro protein synthesis, in a low-cost, easy-to-use, and easy-to-transport manner. The
response signal of the paper-based biosensor can be detected by the naked eye or a digital
camera, and, therefore, does not require high-cost conventional laboratory instruments, making it
especially suitable in low-resource localities.
Timeline:
November, 2013 Topics:
2016 ICCA-LRI and NIHS workshop “Development of novel biosensors for detecting environmental
pollutants”

Publications:
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Tran Thi My Duyen, Hideyuki Matsuura, Kazuki Ujiie, Misa Muraoka, Kazuo Harada, Kazumasa
Hirata: “Paper-based colorimetric biosensor for antibiotics inhibiting bacterial protein synthesis”,
Journal of Bioscience and Bioengineering, 123(1): 96-100, 2017
氏家和紀、Tran Thi My Duyen、松浦秀幸、村岡未彩、原田和生、平田收正: 「タンパク質合成阻害作用を持つ抗菌性物質検出のための紙を基盤とする比色センサーの開発」、第 65 回日本分析化学会年会、札幌、2016 年 9 月
Tran Thi My Duyen, Hideyuki Matsuura, Kazuki Ujiie, Misa Muraoka, Kazuo Harada, Kazumasa
Hirata: “Paper-based colorimetric biosensor for antibiotics targeting bacterial protein synthesis”,
第 68 回日本生物工学会大会、富山、2016 年 9 月
Tran Thi My Duyen, Kazuki Ujiie, Misa Muraoka, Kazuo Harada, Hideyuki Matsuura, Kazumasa
Hirata: “Development of paper-based biosensor for detection of antibiotics targeting proteinsynthesis”, Biosensors 2016, Gothenburg, Sweden, May 2016