P‑2Gene Engineering Division
RIKEN BioResource Research Center

RIKEN DNA BANKclone name : pKOM-7

a Clone lD

RD8 07267

O Lot07267

a DNA Concentration

25 nanogram/microlitera volume40 macroliter

O Form

DNA solulon ln TE buffera Hosta Culture

DH5 alphaa Antibiotics

100 microgram/ml Ampicillin

A8F5

LB medium目 ３ ●{kb)︼工Ｅ ●∞

250 ng/we‖ｏ壼∽うＯ Ｃ コ(NEB#N3200L),

７６ＡＶl kb PIus DNA Ladde「一QIAGEN QlAprep Spin Miniprep kit
a Purificationa Digestion by restriction enzyme

1003040301512¨一一一E‐ ̲̲=■0Electrophoresis i

100

̲=

nanogram DNA per lane ; 1% agarose gel , 1 x TAE Buffer

Restnc‖ On enzyme

Expected size of fragment

SalIBamHI

7.8
5.■ ′2.7

BgiII

5.6′

2.2

kbp
kbp
kbp
kbp

O Confirmation of the insertion sequence
Please be sure to check our sequence analysis results before your request.
Sequence name

P甫

merlD

Primer name

Confirmed feature

Sequence - A
Sequence - B

Pr01 00

reverse-2

insert 5'

Pr0410

M1340

insert 3'

Sequence - C

P「0566Bkvrus Rl

insert mid

Sequence - D
Sequence - E
Sequence - F

APPROVED BY:

http://dna.brc.riken jP木薇躾合賦Bttnls

10235

1

【￨￨

127

19i;十154

163

181

262

271

280

289

73

298

82

CTTC

307

:nst Modelノ Name 3500/3500 instrument」un 08,201801:51PM,JST」un 08,201802:33PM,JST
Spacing:10 98 Ptsノ Pane11 350

P!ate Name:20180608 dnabank

91

316

460

352

577

469

361

586

478

370

595

487

379

604

496

388

613

505

397

622

514

406

523

631

415

532

640

424

541

649

433

550

100

442

667

101

1GTA

559

Electropherogram Data Page 1 of 2658

ヽGTTTTTTCI到

TT TTCTTTTC GTTCGGCCCAT208217￨

TG T TATA G CAT C TA C CC CA G T T T TAAq

199

ハ ノ滑 じ :1多 δδ
・190

GG AGCTCCCCC TCC ACACTCTCC

polTo.,.txV,rヽゃ6407267 07267 A8F5 1 Reverse2 C03 09 AB124
07267 07267 A8F5 1 Reverse2

KB 3500 POP7 BDTv3 mob
Pts 1498 to 12347 Pkl Loci1475

I ぽ■ ,,1b00
爪慮
2F X♭ Й供τ う145

TAGTTTTCCCACTTC CACGG GTTCCTT TG CCTTTTTG

253

CAGTCCCG TACACCCCTACAACCAAAGCCAACATCCATTCACC ACCAGTTCTTTCATTACCACCTCCTGGGGCAAT:晃136

TCCT CCTTTTATTAG

244172

Version 6 0 HiSQV Bases:951
CCCA T TTCT C G AT TTA CAA ACCA TTCT̀c C TTc CAT CCCTGCハ G G TCG ACTCTAC A.

primer name ハ :reverse2
5LGCGGATAACAA丁 丁TCACACAGG‑3'

A CA T C ATIFC C C

118

TCTAC AACTTCTAC洛証343568

GTTTCTTCCAAAAATCTACCCAAGGAGTCTCTAAAGAATCTCTCTCTTCTTCTTTGCAATTCTTGTGAAGTTATACCACGTATATCATCCCTAATAACATGCCACAAAG451〕TTCCCTTTCACCAACTTCCCTAACCATTGAACGCCTAATACGGGACAAATTAGAATAATAATCCTCAATATAATTATAAAACTTTATACGCCCATTTACAATTGTCC

1fAAACTCTCCTGAATCTACATTCTCCTTATTTCTTAACTCCATTCTTTGGGTAACTTCTTGTATACTGTCAGCATCATCTATTCTGTAGGTATGGCCAAAATTTACTTGGGITC(こξマC TTCATATC CT TCAACACCACGTGTTA

9】T1AハCap:9S/NG:135A:262T:316C:326
KB bcp

KB14181332
66601332
66601332
66601332
666
01332
66601332
666
0Printed on:F「 i」 un 08,201804:35PM,JS丁IJЦ3)1箋

r7ts

薇 B島躍語
き5784

685

793

892

694

802

703

901

991

712

721

730

Pts 1498 to 12347 Pkl Loci1475
Version 6 0 HiSQV Basesi 951

07267 07267 A8F5 1 Reverse2 C03 09 AB!2407267 07267 A8F5 1 Reverse2
KB 3500 POP7 BDttv3 mob

811

820

829

1072

T C

1009

TT A TC C

919

AA TT A AAGCAGCAAACCCAG
910

1000

ACG928838

739

CC C C

1847

1081

ハAG748CClnst Modelノ Name 3500ノ 3500!nstrument」un 08,201802:33PM,JS丁」un 08,201801:51PM,JST

Spacing:10 98 Ptsノ Pane11 350

ACAA AAT]ACATAGTA:71

Plate Name:20180608̲dη abank]ム

85396ィ

1027

1865

1090

AA T TT T T C A

TAc CAA GGG

955

AAT TACAC CA綿′石下A G

Tc TACAG

946

TCG ACCACC AG C

DF″ ￨が937

C AA T AC CCCCC

G T

９982

1054

Electropherogram Data Page 2 of 2︲ ︲ ︲ ︲ｌ Ｊ ６

973

1045

1063

TTTTC A GC AG TTA CG CC TA TAG C AG C T A T AC CC TTC TG A CG TTAC T TG

883TC AGC CAAAGAA CTAA TAC CA GT AA CACTTTTGハG・lCAｆ4『41099GATC C A TTTT C A C TTT C TTA TA G C AC C ACA G08,201804:35PM,JST1036

C Al C TG G6 T TAAA CAA T T C CAA TG C CA TG C C T G AT TG C T G AT AAAGG C C T AC AG TG G A AAC T T TG T G AT C CC AA T C AC 'T AAAAAAJAC C T A T A C C C TJ676

CCTCCGAAATAGTAGCAAACAAACAACCACCCCAATGCCTAGGATCTAGGTATTCAATATTATTTACAAAAGTATTTACACCAGGCap:9NG:135A:262丁 :316 Ci326KB bcp

SノKB1418

133266601332
66601332
66601332
66601332
66601332
666
P面 nted on:F‖ 」un漑島蜃畷卜8輿 rns
β :M13̲40

Spacingi10 97 Pts/Pane11 350」un 08,201801:51PM,JS丁

「lT G

172

6473■ )お〕

181

190

613

GGG TG TAGG GC

397

TACC CC

514

289

631298640

532307649913164331]」un 08,201802:33PM,JST

217

559

334

ACA G ttG G T TAT G

325442ATCAATACT CACCATAAC GCCTAC

550

AATGTTCCCCCACTAC

667

Electropherogram Data Page 1 of 2658

TCAG CTCCT CATATTTGT GGAC TG

541

CATACACAG C ACGGGAATC CCACACCTCAG TCCCACGT406415424523

AAAATACTAGATATTTT GGA′622

CTCTG TGTAAACCT CATACCC TG TATGTT208

CT C CC TTTC ACn GTC ATAG:∴ CAC ACAG]∬

82199

TC TAACCTG TGG AAATC TACTAATG TGGG ACGCTGTC iCTGTAAAム55

ACCT TAC G GG CTACAG TCTAAG′46163

lnst Modelノ Name 3500/3500 instrument

07267 07267 A8F5 1 M13 ‐40 D03 12 AB!244007267 07267 A8F5 1 M13 ‐KB 3500 POP7 BDttv3 mob

P:ate Namei 20180608̲dnabankpttmer nameC G T

154

CTAAC TG CTC A:Pts 1505 to 12343 Pkl Loc:1482

S/NG:102A:186T:184 Ci186

o R1 145

604496

TTCCTGACCCTAGTAGAAATG
487

TCCTCGハACTACACAACAAAGTACCCA GAACGTACT GT
370379388

13613ι

361

478

595

505

AGAATCGTGGACCCAAACCTATTCAAGGCACCAATTTTCACTTTTTTCCTGTGGGTCGGGACCCCAAC A■ T TCCACTAC CTAATTTGAATGAG GハG TAC C G G

244

FG■ GCTCG

19α127TATGCT TA,〈,Pc1235

352586TACCACAG TG TTGC TC GAT GAAC「469

CAATCCT TATCCAGTCGA577

Vers!on 6 0

HiSQV Bases:963

5LGTttTttCccAGTCACGACGTttGTA‐ 3:

Cap:1210

CA CC(C (CGハ AT■568

TCATGTAACCAACACACC

343

TTGG AAATCCAGGGAGTACTTATGA226〕GAATAACTAG118

AT CCTTCCCTG TTACAC CACAG

1崎こ卜01
PA・ "中ザDf yolyouvtf,Virag 0N(rKB bcp

KB14181164
58201164
58201164
58201164
58201164
58201164582
0
Printed on:Frl」 un 08,201804:35PM,JS丁鼈 合Bttrns

685

S/NG:102A:186丁 :184 Ci186

Cap:12676784

BB3AGT CAC CTTA

l108

CC973

CGTT AT TFTG

45

TT T T A
1054694Bs2982

703

ム GTG

901

1063

T T IIT T A AA A C A C TC

CAlnst Modelノ Name 3500ノ 3500:nstrument」un 08,201801:51PM,JS丁」un 08,201802i33PM,JST

1000

1072

GTTTT A C A

9r-9

CG

92a

ι口1018

g3't / t

TT A TTTTT C G CC G

T CCG

1090

CC

946

A A

1027になγ
レ嘔F万れこ
1009

T G

865

T TTCATG GCACAG

9ss1036

1099

874

964

AA CT

1

Electropherogram Data Page 2 of 2

T TC T T T CC

1

Spacingi10 97 Pts/Pane11 350

C77507267 07267 A8F5 1 M13 ‐40 D03 12 AB!2407267 07267 A8F5 1 M13 ‐40
KB 3500 POP7 BDTv3 mob

730

856

AG GTCAC GG TG

766

Piate Namei 20180608 dnabank

Verslon 6 0721

CiGC C TrlT G T′ 、TGGT「 、TG GACTC TCAC GTCG AG G
820
829838847757HiSQV Bases:963748

Pts 1505 to 12343 Pkl Loc:1482

712

GATCGG

910

AT ATA TTG ACAG AC AGGG AC!ATTGCAAACAAAAdATGGT?TAAACAAGGTGCTTT811991ACTG TACAT ATAC ATC C TTA AT AAA TC C T C C TTTTT A T A TT AC A C A TTTT AA T C TTT C T GAC AGC TT CC AGG GG ACCCAG ATATCATAAGTAAACACC AC AACCCAG AG
793802ACTAACAGCTCTCCAACACAACAGTCCAGGGCCCTTCCAACATATTTTAAGATTCCCCTCAGAAAAACATCTGTGAACAACCCTTACCCAATTTCCTTTTTCCTTI739)路KB1 41.8

KB.bcp1164
58201164
58201164
58201164
58201164
58201164

Printed oni Fri」 un 08,201804:35PM,JST合8輿 ms:BkV「 us̲Fl」
が｀2911Dl, 19̀‑ 3244253

TTTTCTTi■

28

江=つ145262

ムT,￨■ iTTTTユ370

37

KB 3500 POP7 BDTv3 mob
Pts 1570 to 12066 Pkl Loc:1547

46

A/ A}2tltDt'l

380

55

172

64181

631

73

CT T C AAAAACT415IC82424I541

649

Gll.TCTlNlcυl334

559

667

TT4

Electropherogram Data Page 1 of 2658

TC TTTTACCTCACAT

TTl.TTCCム TG TCCTG● ACG
442451325

CTCム TTCi TTGT AiC TA■ li C Cl

208

C TCACAATi:ξ
91

Plate Name:20180621 dnabank
CCCTTT::9TI

Spacingl10 85 Pts′ Pane11350」un 21,201802:36PM,JST」un 21,201801:54PM,JST

lnst Model′ Name 3500/3500 1nstrument

C l i

199

433

Cへ CG,Tム

316

TCCCTTAAACTGTCTAAATTCT:,C CI‐

TCi:=「 .■ GTF!∴ GC CT TTCCATCC GTAAAT

532640G ACCT TTC AACA

523307CA■ ATc TAcc CTCCFiム CG TTTTCCGGた514

298

289397622406280505613

r GT TTTTCCiC T,、 rCiG

TCTGTCTT■

163

ハATCTム154271

CCTCT'ム

379

TCATC

07267 07267 A8F5 1 BKv「 us Fl F03 18 AB124
07267 07267 A8F5 1 BKvirus F4p雨mername c

S/NG:350A751T1094 Ci991

5'GGGATTCAGTGCTTCATCCATG 3'

GTC T

Capl18AT(G235361

GTTCTTTTCTTTAACTGTTTCTTTTCTAAATTAACTTTC ACACTTCCATCTAAATA

496

TTCCCTTCACATCTTCAAAAACAACCATATACTCATCTATAcccACACC CAGTuon

487478

Yg-tl

469

595

ql_G_g_a-G.c r AGrGr586

CACATAAAT CTAA CAACCiTTCAC CTCCTC577

TTCC CTC日ヽAd匝568

ム ACCT460

AAATCCTTTG'C 343
CTCCAAATAT TTCG35226

TTCTCAGTTTTCCAGGG■

χ際「 1 136rccATT cAAcAATTC TAC ACT GTATATC TTCTCCAAAATCACC TACACCCCT C AACCAAATTA CCAC TACCA

118

Verslon 6 0 HiSQV Basesi 920
lC T TC TTCIIC TT CTl,TAT Cハ A C ArTACAT TT T cCCATC CATATAT TATAT T

KB bcp

KB14181090
54501090
54501090
54501090
54501090
5451090
545

Printed On:Thu」 un 21.201804:23PM,JSTS′Bttms
合KB 3500 POP7 BDttv3 mob

07267 07267 A8F5 1 BKvirus Fl F03 18 AB12407267 07267 A8F5 1 BKvirus Fl

Cap:18730739748」un 21,201802:36PM,JST

lnst Mode:/Name 3500/3500:nstrument」un 21,201801:54PM,JS丁Ｔ

847

ＴＣＡＧＡ

６６７856ＴＴ

α

AGGG TATC TACCＣ1054

TT

露聖⊆』

AG GノCT

86

lぜン
γ蹴ゼコ

まだA

Electropherogram Data Page 2 of 2=見N↓ e工二Ｇご三=L三C CC (T1000

T TT T T A

838

T C A C A C「■1045

７５Tr. T

Ａ

991

TTC

TTC TTCTCTCG TCATATGG

Spacing:10 85 Ptsノ Pane:1350

TT

919

P!ate Name:20180621 dnabank

820

T910

T C

T

HiSQV Bases:920
721

II T CGAA A

APts 1570 to 12066 Pkl Loc:1547

Version 6 0

811

901

982

C C

1036

Ｃ712

T A

Ｔ

TＴ

892

AGA A CG T GG
973

A A
1027

Ａ703

802

TTTATCTA ATATAT C ATTA AATC

T C

Ｃ

694

793

883

T A CC

Ｇ964

CＴ

685

784

T829

NG:350A:751T:1094 Ci991

KB bcp

676

874

1018

T A C ATG TCTCTTAC CATATACAGTCCACCAAATCAAATATAACACAATCCATTTTACGTACCAAACAGTCCAGCCACCCAACTCCTCCCATAT775

G ACCTCCAAATATTAAATC CAT
9α c I955

T CTTTTTT'TAGCTAACACTGTATCCACTGCTTGCTCACAAATACTTTTTTTCATTTTTTTAC TTL贔CGC AAAA A T G C TTTTT C A T G A TAA T1til)6,3/1

KB14181090
54501090
5450109‑5451090
54501090
5450

Printed oni tthu」 un 21,201804:23PM,JST