Estrogen Receptor Alpha Antibody
Rabbit Polyclonal
Antigen Affinity Purified

Protein ID

P03372

Catalog No.

A300-498A

GeneID

2099

Lot No.

A300-498A-3

APPLICATIONSWB, IP

SPECIES REACTIVITY

Human

AMOUNT

100 µlCONCENTRATION

1000 µg/ml

STORAGE/SHELF LIFE

2 - 8° C / 1 year from date of receipt

PHYSICAL STATE

LiquidBUFFER

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

ISOTYPE

IgG

ORIGINUSA

PRODUCTIONPROCEDURES

Antibody was affinity purified using an epitope specific to Estrogen Receptor Alpha immobilizedon solid support.
The epitope recognized by A300-498A maps to a region between residue 550 and the Cterminus (residue 595) of human Estrogen Receptor Alpha using the numbering given inSwissProt entry P03372 (GeneID 2099).
Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm
of 1.4 equals 1.0 mg of IgG.

APPLICATIONSAPPLICATION NOTESCentrifuge tube to remove product from lid. Optimal working dilutions should be determined
experimentally by the investigator. Prepare working dilution immediately before use.
Western Blot

1:2,000 - 1:10,000

Immunoprecipitation

2 - 10 µg/mg lysate

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020),
Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE(link to IP-western blot protocol in Additional Info section below).
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

ADDITIONAL INFO

https://www.bethyl.com/product/A300-498A
Use the link above to view SDS, a current list of citations, and other product specific information.
IP-western blot protocol: https://www.bethyl.com/content/protocol_IP_WB

This document certifies that this product has met all of the quality control standards defined by Bethyl Laboratories, Inc.
Eric McIntush, PhD | Chief Scientific Officer
Date: June 21, 2019

Bethyl Laboratories, Inc. • 25043 West FM 1097 • Montgomery, TX 77356 •800.338.9579 • 936.597.6111 • 866.597.6105 (FAX) • www.bethyl.com • technical@bethyl.com

1For in vitro laboratory use only. Not for any clinical, therapeutic or diagnostic use in humans or animals. Not for human or animal consumption. This product may not be resold or modified for resale without the prior written
approval of Bethyl Laboratories, Inc. The information provided in this data sheet is believed to be correct but does not purport to be all-inclusive and is intended to be used as a guide. Bethyl Laboratories, Inc. shall not beliable or responsible in any way for use of either this information or the material supplied. Disposal of hazardous material may be subject to federal, state or local laws or regulations.

Estrogen Receptor Alpha AntibodyDetection of human Estrogen Receptor Alpha by westernblot. Samples: Whole cell lysate (50 µg) from MCF-7 cells
prepared using NETN lysis buffer. Antibody: Affinity
purified rabbit anti-Estrogen Receptor Alpha antibody
A300-498A (lot A300-498A-3) used for WB at 0.1 µg/ml.
Detection: Chemiluminescence with an exposure time of30 seconds.

A300-498A

Detection of human Estrogen Receptor Alpha by western
blot of immunoprecipitates. Samples: Whole cell lysate(0.5 or 1.0 mg per IP reaction; 20% of IP loaded) fromMCF-7 cells prepared using NETN lysis buffer. Antibodies:Affinity purified rabbit anti-Estrogen Receptor Alphaantibody A300-498A (lot A300-498A-3) used for IP at 6µg per reaction. Estrogen Receptor Alpha was also
immunoprecipitated by previous lot of this antibody
A300-498A-2. For blotting immunoprecipitated Estrogen
Receptor Alpha, A300-498A was used at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of
30 seconds.

Bethyl Laboratories, Inc. • 25043 West FM 1097 • Montgomery, TX 77356 •800.338.9579 • 936.597.6111 • 866.597.6105 (FAX) • www.bethyl.com • technical@bethyl.com

2For in vitro laboratory use only. Not for any clinical, therapeutic or diagnostic use in humans or animals. Not for human or animal consumption. This product may not be resold or modified for resale without the prior written
approval of Bethyl Laboratories, Inc. The information provided in this data sheet is believed to be correct but does not purport to be all-inclusive and is intended to be used as a guide. Bethyl Laboratories, Inc. shall not beliable or responsible in any way for use of either this information or the material supplied. Disposal of hazardous material may be subject to federal, state or local laws or regulations.