NATIONAL INSTITUTE FOR BASIC BIOLOGY& il ~ •~iJf !: ifi

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ANNUAL REPORT

1988

BRIEF SUMMARYThe National Institute for Basic Biology, NIBB, is a part of the Okazaki
National Research Institutes located on a hill overlooking the old town of Okazaki.
Its activities are supported by Monbu-sho (The Japan Ministry of Education,
Science and Culture). The research institute is composed of three independent
organizations, the National Institute for Basic Biology (NIBB). the National Institutefor Physiological Sciences (IPS) and the Institute for Molecular Science (IMS).
NIBB was established in 1967 and it celebrated its tenth anniversary in 1987.
NIBB is an interuniversity research institute with its own research programs
as well as cooperative programs to promote basic biology at the national andinternational level. The programs are 1) research projects at each division 2) joint
research programs in which university scientists are invited to participate inresearch projects with NIBB members, 3) facility-sharing programs in which
university scientists utilize the institute's research resources, 4) graduate student
training programs in which graduate students from universities spend fixed
periods of time with the NIBB, and 5) international programs in which foreignscientists are invited to NIBB to conduct research projects. NIBB sponsors"Bioscience Training Course" of about 7-10 days for selected people from allover Japan.

PROFESSOR T. S. OKADA

2INTRODUCTION
The National Institute for Basic Biology (NIBB) aims to facilitate
basic research in the biological sciences in Japan by conducting
advanced studies to elucidate various fundamental mechanisms
underlying living organisms. Research areas include studies of cell
growth, development and differentiation. and the control of various
cell functions among others. The ultimate goal is to understand themechanisms of phenomena in eukaryotic organisms at the molecular level. Analytical approaches include biophysical. cell biological.
and molecular techniques. incorporating modern methods of genemanipulation.
The NIBB. is an inter-university research institute, and as suchhas a two-fold mission: (1) to conduct in-house research and (2) tomake the facility available for collaboration with scientists outside
the NIBB. Thus, the NIBB is an "open" institute. The Institutecontains three departments, Cell Biology, Developmental Biology,
and Regulation Biology, which are divided into 13 divisions. Eachdivision is staffed by a full professor, an associate professor andtwo research associates. Six of the professional appointments areadjunct professorships; these individuals have joint appointments atother institutes in Japan. The NIBB sponsors joint research
programs with participating individuals or research groups nationallyand internationally.
The NIBB provides an opportunity to share research resourcesamong biologists in Japan and from abroad. It also sponsors
symposia on current topics at the interdisciplinary level by inviting
participation by leading scientists in various related fields. both frominside Japan and abroad. Thus, the NIBB is becoming and willcontinue to be an internationally recognized institute for conducting
basic research in the biological sciences.
This report consists of an outline of activities of the NIBB andof a list of papers published by the members in the 1986-1987
period.

T.S. Okada. D.Sci.
Director General

3

ORGANIZATION OF THE INSTITUTEThe Okazaki National Research Institutes. ONRI, are composed of three individual Institutes. NIBB. Institute for Molecular Science (IMS). andNational Institute for Physiological Sciences (IPS). The president of ONRI is Prof. Saburo Nagakura. IMS and IPS are headed by Professors Hiroo
lnokuchi and Setsuro Ebashi, respectively.

Policy and Decision Making
The Director General oversees the operation of the instituteassisted by two advisory bodies. the Advisory Council and Steering
Council. The Advisory Council is made up of distinguished scholars
representing various fields of science and culture. and advises the
Director General on the basic policy of the institute. The Steering
Council is made up of professors of the institute and an equal
number of leading biologists in Japan outside NIBB, and advises the
Director General on the scientific activities of the institute. The
Council advises on faculty appointments and on the institute's
annual budget as well as its future prospect.

Ad ministration
Administration of the institute is undertaken by the Administration
Bureau of the Okazaki National Research Institutes under the direct
auspices of the Ministry of Education, Science and Culture.
Currently the chief administrator is Mr. Shousuke Wakisaka.

Research
The institute conducts its research programs through three departments organized into 13 divisions. Each division has its own
research project and is staffed by a professor. an associate
professor and two research associates. A division forms. inprinciple, an independent project team. Half of the divisions are for
adjunct professorship and are under professors who hold joint
appointments with other universities. The adjunct division has

4

resident research associates. The arrangement aims to facilitate
exchange in research activities in Japan.
The technical Department manages the activities of researchtechniques and helps to promote research activities of each division
and also to maintain the research resources of the institute. The
department undertakes the technical education of its staff.

Research Support Facility
The research support facility of NIBB consists of the Lar,ge-scale
Spectrograph Laboratory, Tissue and Cell Culture Laboratory, Laboratory Computer Facility, Plant Culture Facility, Plant Cell Culture
Facility, and Experimental Farm. In addition, seven facilities areoperated jointly with the National Institute of Physiological Sciences(IPS); they consist of the Radioisotope Facility, Electron Microscope
Center. Center for Analytical Instruments, Machine Shop, Laboratory
Glassware Facility, Animal Care Facility, and Low-Temperature
Facility.

CampusThe Okazaki National Research Institutes cover am area of 150.000
m 2 with four principal buildings. The NIBB's main research building
has a floor space of 10,930 m 2 . Two-thirds of the space wascompleted in 1982 and the remaining third in June, 1983. The
buildings which house the researrch swpport facility were also
completed in June 1983.

DEPARTMENTOF Clill BIOLOGY
Chairman: Yoshihiko Fujita
Two research divisions and three adjunct research divisions belong
to this department.

Division of Cell Mechanisms
Professor: to be appointed
Associate Professor: Toshiyuki Nagata
Research Associate: Kazuo Ogawa
The mechanism of transmission of cellular organelles to the next
generation has been pursued in this division for several years.
These studies revealed that shortly after zygote formation paternalorganelle genomes are digested by Ca 2 + -dependent nucleases
directed by the genetic information from the maternal nucleus in an
isogamous organism such as Chlamvdomonas reinhardtii. This typeof organelle trasmission has been found to be widely distributed
among lower organisms and should be the explanation of maternal
inheritance at the molecular level. On the other hand, althoughmaternal inheritance was first discovered from the study on genetic
crosses among various higher plants in 1907 by C. Correns, noappropriate explanation has yet been given. The efforts of thisdivision in the last year. employing detailed observations using
fluorescence microscopy after staining with DAPI, a DNA specificfluorochrome. clarified that organelle nucleoids disappeared at a
specific stages during the formation of male generative cells in awide range of higher plants. Such results revealed in heterogamousorganism made a more unified explanation of the mechanism ofmaternal inheritance possible.
The replication mechanism of cytoplasmic genomes thus inherited

to the next generation has also been pursued . It has been foundfrom the study of cultured tobacco cells that rapid plastid DNA
synthesis started soon after transfer to fresh medium. and this DNA
synthesis preceded nuclear DNA synthesis. This separation of thesynthesis of plastid DNA from that of nuclear DNA enabled us toanalyse the mode of plastid DNA replication, which has not been
elucidated yet, while its whole nucleotide sequence data are
available.
It has been established from the previous study by this division thatplastid nucleoids have a highly organized structure. Biochemical
analysis of this plastid nucleoid showed that four proteins separated
from the plastid nucleoid are DNA binding proteins. which aresupposed to regulate the functional expression as well as the
structural integrity of plastid nucleoid.
Thus this division is devoted to the elucidation of the interaction
between the cytoplasm and nucleus at the molecular and celluar
level in several organisms.

Deparment of Bioenergetics
Professor: Yoshihiko Fujita
Associate Professor: Shigeru ltoh
Research Associate: Mamoru Mimuro
Kaori Ohki
Photsynthetic energy conversion has been investigated at thecellular and molecular levels. Our research is currently focused on(1) regulation of stoichiometry between two phototsystem (PS)complexes in response to the light regime for photosynthesis and(2) reaction mechanism and molecular structure of thylakoid protein
complexes functioning in the primary process of photosyntheticenergy conversion. Current progress in our research is as follows:(1) Regulation of thvtakoid composition : Our previous study has
revealed that in the cyanobacterial system, stoichiometry between
two PS's is regulated responding to the light regime for photosynth-5esis: the ratio of PS 1/PS II becomes larger under the light mainly
exciting PS II while it is reduced under the light mainly exciting PS
I. Determination of the contents of PS I, PS II and cyt b6 1f
complexes in a single cell has revealed that the content of PS I
complex is regulated. Further study has indicated that the synthesis
and/or the assembly, but not the decomposition. of PS I complex isregulated in response to the light regime . These findings havebrought about an idea that the synthesis and/or the assembly of PS
I complex. the terminal component of thylakoid electron transportsystem is controlled so as to balance turnovers of two PS's.
(2) Reaction mechanism and molecular structure of light-harvesting
antennae: The study on the excitation energy transfer withinlight-harvesting antenna is currently focused on the energy transferfrom light-harvesting antennae to the reaction centers in higher
plants. Kinetic as well as spectral analyses of time-resolved
emission spectra at ps range were made with isolated PS II
particles and PS II complexes variously resolved. Analyses havesucceeded in the establishment of a model for the energy transfer
path from chi's in LHC II to the PS II reaction center chi via thosein 43 and 47 kd peptides of PS II complexes. The structural study
on phycobilisome (PBS) antenna has been made by electron
microscopic analysis of in vivo and in vito PBS as well as analysisof the pigment composition in a single PBS. The study has brought
about a finding of a structurally new type of PBS.
(3) Reaction mechanism in PS I complex: The electron transportmechanism in PS I complex has been studied with isolated PS I
complexes by analysis of rapid electron flow kinetics induced bylaser-flash and of EPR signals. Special attention has been paid tothe function of vitamin k-1 in the electron transport after the charge
separation in PS I complexes . Based on the finding that
diethylether-treatment efficiently removes not only chi a but also
vitamin k-1 from PS I complexes, the electron flow kinetics wereanalyzed for the ether-treated PS I complexes before and after
adding back vitamin k-1. The analyses have provided a new finding

6

that vitamin k-1 is the secondary electron acceptor mediating theelectron flow from the primary to the terminal acceptors in thecomplex.

Division of Cell Proliferation
Professor: Yasuhiro Anraku
Associate Professor: Souichi Nakamura
Research Associate: Hidetoshi lida
Versatile roles of calcium signaling in the regulation of cellproliferation of the yeast Saccharomyces cerevisiae have been
investigated. The research is currently focused on the following two
projects. 1) An experimental system suitable for yeast cell culturing
under calcium-limiting conditions has been established. This system
make it possible to investigate how the initiation of DNA synthesis.
budding and mitosis is regulated by Ca 2 + and to isolate mutants
which cannot grow in calcium-deficient medium. 2) Techniques formeasuring intracellular free Ca 2 + in individual yeast cells have been
established. We have employed fura-2 as a calcium-specific fluorescent probe in conjunction with digital image processing. We havefound that fura-2 can be effectively loaded into yeast cells byelectroporation without significant loss of viability. Images of fura-2
fluorescence under the Nikon Microphot-FX microscope are acquiredby a SIT camera and relayed into a TV monitor and the Hamamatsu
Photonics Argus-100 image processor. Using this system. we haveshown that the intracellular free Ca 2 + concentration in cells of amating type increases 5-8 fold after 1h-incubation with a yeast
mating pheromone, a factor that is known to arrest cells in the G,
phase of the cell cycle .

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Division of Cellular Communication(Adjunct)
Professor: Voshiro Shimura
Associate Professor: Kenzo Nakamura
Research Associate: Kiyotaka Okada
The division deals with molecular biology of higher plants. Ourcurrent interest resides in the genetic control of flower development and morphogenesis, and of gravitropic response in a small
crucifer, Arabidopsis thaliana. Arabidopsis thaliana is well suited forgenetic and molecular biological studies. since this plant has some
remarkable features such as exceptionally small genome size (7 x
107 base pairs per haploid which is only 20 times that of E. coli).
short life-cycle (5-6 weeks). small size of plant body (20-30 em inheight) and easy cultivation in laboratories under continuous illumination at a constant temperature (22°C).
We have isolated a number of mutants which show aberrant
phenotypes in flower morphology and/or development and in root
gravitropism. These mutants have been analyzed genetically, so thatthe number of mutational loci. dominance and allelism have beenclarified in at least some of the mutants. In addition to the searGhand characterization of the mutants described above. we have also
isolated clones carrying the calmodulin genes of the plant. Thesestudies will be detailed in the following section:
The developmental and morphological processes of flowers consistof at least several stages : (1) generation of floral primordia. (2)arrangement of primordia of floral organs (sepal, petal. stamen andpistil). (3) development and differentiation of the organ primordia, (4)maturation of each floral organ, (5) blooming and (6) fertilization. Of
some 150 independent flower mutants of Arabidospsis isolated, we
have found some mutant strains whose genetic defects seem tocorrespond to each of the stages described above. For example. amutant which generates no flowers on inflorescences, mutants
which lack petals and/or stamens. and mutants which have

8

abnormal structure in pistils could be interpreted to have abnormalities in stages (1). (2). and (3). respectively. Of particular concern is aclass of mutants which have homeotic conversions between floralorgans. For example, sepals are converted to pistils in one mutant
strain, and petals to stamens and sepals to leaves in anothermutant. These mutants could be caused by a genetic defect in adevelopmental process which determines the fate of an organ
primordium . Genetic analyses of these mutants clearly show thatmost of these flower mutants carry single, recessive, nuclear
mutations.
We have also isolated at least 10 mutants which show an abnormal
gravitropic response in root elongation. The mutants are classified
into at least three groups phenotypically : a group showing delayed
responce, a group which does not respond to a change of gravity
direction, and a third group which does not grow straightly
downward but fluctuates mincingly. Genetic analyses of the gravitropic mutants are in progress.
Calmodulin is known to be a ubiquitous regulatory protein in higher
and lower eukaryotes. Cloning of calmodulin genes from an A.
thaliana genomic library has revealed that there are at least a few
non-allelic calmodulin genes in the plant. The nucleotide sequencesof the two indicate that they are highly homologous to each otherand are also quite similar to the calmodulin genes of othereukaryotes. The exon-intron organization is almost identical in thetwo non-allelic genes of the plant but is considerably different fromthat of other eukaryotes including yeast. trypanosome and mammals.
Attempts are being made to identify and isolate the genes
responsible for the flower mutants as well as the gravitropic
mutants using transformation systems mediated by the Ti-plasmid
and Ri-plasmid vectors or by a direct gene-tagging system. For such
experiments, it is absolutely necessary to develop a good, efficienttransformation system and also to construct a good genomic libraryof the Ti-derived cosmid vector.

Division of Cell Fusion (Adjunct)
Professor: Tsuyoshi Uchida
Associate Professor: Masaru Vamaizumi
Research Associate: Masahiro lshiura
Kenji Kohno
Molecular and cellular biology of mammalian cells. aided by cellengineering and recombinant DNA technology, are the research
projects of this division. In relation to the action mechanism for
diphtheria toxin in mammalian cells. the structure and function of
the elongation factor-2 (EF-2) involved in the protein synthesizing
system of mammalian cells are currently being extensively studied
at the molecular level using cloned EF-2 genes. Cosmid cloning has
been established by constructing simplified cosmid vectors andselecting adequate E. coli. Elucidation of the mechanism for DNA
repair in mammalian cells is also in progress.

DEPARTMENT
OF DEVELOPMENTAL
BIOLOGY
Chairman: Goro Eguchi
The department has three research divisions and one adjunctresearch division which conduct research into the cellular andmolecular mechanisms of various processes involved in developmental phenomena .

Division of Reproductive Biology
Professor: ¥oshitaka Nagahama
Associate Professor: to be appointed
Research Associate: Michiyasu Voshikuni
The division conducts research on the molecular mechanisms ofdifferentiation. growth and maturation of gonads (ovary and testis)in multicellular animals. particularly the hormonal control of oocyte
growth. oocyte maturation, spermatogenesis. and spermiation.
These studies combine biochemical. ultrastructural and physiologicalapproaches. Our current investigations are designed to identify andcharacterize the molecules which regulate ovarian and testicularfunctions. This information will provide the basis ~or a study at themolecular level of their precise mode of action .
Research centers around oocyte maturation in teleosts and starfish.
Our previous studies have established that three major mediators
are involved in the regulation of oocyte maturation in these animals :gonadotropins (GTH) or gonad-stimulating substances (GSS).
maturation-inducing substances (MIS) and maturation-promoting factor. or M-phase promoting factor (MPF) . These three mediators
function sequentially at the levels of the follicle layer. the oocyte
surface and the oocyte cytoplasm. respectively. In 1985, we
identified for the first time in any vertebrate the MIS of salmonid
fishes as 17a.20f3-dihydroxy-4-pregnen-3-one (17a,20f3-DHP). Production of this steroid occurs via the interaction of two distinct cell
types in t~e follicle and is controlled by GTH . We are now focusing
on the molecular mechanisms involved in the svnthesis and actionof 17a,20f3-DHP.
Our current research concerns (1) the characterization and/or
synthesis of teleost GTHs and starfish GSS. (2) the molecularmechanisms of biosynthesis of salmonid MIS, 17a.20f3-DHP, andstarfish MIS, 1-methyladenine, in ovarian follicle cells. (3) the
purification and characterization of GTH receptors located on
salmo11id ovarian follicle cells. (4) the characterization of the MIS

9receptors located on the oocyte surface. and (5) the purification,
characterization and function of fish MPF. Other ongoing investigations concern the genetic control of sex differentiation, and defining
the molecules (proteins and steroids) involved in the initiation ofmeiosis. spermatogenesis and spermiation.

Division of Cell Differentiation
Professor: Voshiaki Suzuki
Associate Professor: Kohji Ueno
Research Associate: Shigeharu Takiya
Toshiharu Suzuki
Members of the division have been analyzing developmental
regulation of the tissue-specific genes and the homeotic genes inBombyx mori. Recently we have described that the transcription of
the silk fibroin gene in the posterior silk gland cells is initiated ataround E25 stage when silk gland morphogenesis has been
completed in the embryos. The fibroin gene is repeatedly switchedon and off in the following stages of larval development. To analyze
the molecular mechanisms of the tissue- and stage-specific regulation of transcription. we have developed 18 cell-free transcription
systems from embryos and several tissues from various developmental stages and cultured cells. These cell-free extracts revealtranscription in a stage- and tissue-specific manner, and differential
transcription of two genes can be demonstrated. Using thesesystems and techniques of gel shift, footprint. and biochemical
fractionation of the extracts, we have detected tissue-specificfactor(s) as well as ubiquitous factors that interact with the fibroin
DNA and sericin DNA. Currently we are concerned with how
synthesis and specificity of the factors are regulated. In Bombyx
mori. more than 20 homeotic mutants that mapped to the proximal
end of the 6th linkage group were described as E-group during theperiod of 1930 and 1960 in Japan. They exhibit a variety ofdevelopmental abnormalities in organogenesis in various body

10

segments, and in most cases homozygosity of the mutant gene
results in embryo death. These genes probably regulate somestructural genes that play important roles in pattern formation. We
have begun to clone some of these homeotic genes in order toanalyze the molecular events during embryonic development andhopefully to clarify the relationship between these regulatory genes
and those structural genes such as the fibroin gene that are
expressed tissue-specifically.

Division of Morphogenesis
Professor: Goro Eguchi
Associate Professor: Kenji Watanabe
Research Associate: Ryuji Kodama
Kiyokazu Agata
Problems of stability and instability in cell differentiation have beenstudied as current projects in Division of Morphogenesis.
(1) Remarkable progress has been attained in understanding thetransdifferentiaton of pigmented epithelial cells(PECs) into lens cells.
Utilizing our established culture system of chick embryonic PECs.
we have cloned genes coding for several molecules characteristic of
PECs and lens cells. Melanosomal matrix protein (MMP-115) is
detected with a monoclonal antibody, and its eDNA is cloned andfully sequenced. MMP-115 gene is expressed specifically in PECs
and skin melanocytes. MMP-115 will be a cue to dissect the ·formation of melanosomes with gene engineering. Two other genes(pP344 and pP64) specifically expressed in PECs are cloned andsequenced and their functions are now being studied. Expression ofpigment cell-specific genes (MMP-115, pP344 and pP64) and lens
cell-specific crystallin genes (a A and /3) are entirely repressed in
the multipotent (at least bipotent) dedifferentiated state of PECs.
Gap junctions are responsible for intercellular communication in theepithelial structure organized by PECs. They diminish in thededifferentiated state of PECs. In normal lens cells and transdif-ferentiated lens cells, another type of gap junction is expressed. We
are purifying both types of gap junction proteins to clarify regulatory
mechanisms of their switching expression in the transdifferentiationof PECs.
Ability of PECs to transdifterentiate was first found in the newt.
Now it is possible to assume that such an ability to transdifferentiate is conserved widely in vertebrates including humans. PECs ofhumans have high growth ability and are able to transdifferentiate
into lens cells under similar conditions used in chicken PECs
culture. Dedifferentiated PECs of humans have been maintained inculture tor more than 6 months. expressing lens phenotype
continuously and are expected to become a cell line.
(2) Although lens regeneration from PECs in situ is possible only ina limited number of species. PECs of various animal species cantransdifterentiate into lens when dissociated and cultured .
Transdifferentiation of PECs in vitro was achieved by manipulationof cellular environments. suggesting the presence of some key
molecules responsible tor stabilization of the differentiated state of
PECs in situ. We have obtained an exciting monoclonal antibody byimmunizing mice with the homogenate of adult newt irises. The
monoclonal antibody -stained the cell surface or intercellular materials of various newt tissues including the iris. Soon after lentectomy, however, antigen molecules began to decay only in thedorsal marginal iris pigmented epithelium, the site of lens regeneration, and became temporarilly undetectable prior to dedifferentiationof PECs. Fragments dissected from the ventral iris, from which lensregeneration never occurs in the eye in situ. became capable oflens formation, when pretreated with the antibody. The antigen
molecules reacting with the monoclonal antibody must be required
for stabilization of the differentiated state of the newt iris PEC, andthe loss of these molecules might be closely related to the initiationof dedifferentiation of dorsal iris PECs prior to transdifferentiation.
(3) Senile cataracts are one of the important problems associatedwith prolonged life in humans. What mechanism underlies in the

12

progress of senile cataracts?. The lens is highly susceptable toenvironmental disorders such as high glucose in the blood, leading
to cataracts. This property of the lens might be due to thecharacteristic structure of this organ . The lens is a large avascular
tissue composed of a sheet of lens epithelium and a mass of lens
fibers. Lens epithelium is responsible for maintaining the innerenvironment of the lens fiber mass. Aging of lens epithelial cells
must lead to deterioration of their function and cause cataracts. To
investigate the aging of lens epithelial cells, we are studying the
growth and differentiation potential of lens cells from humans ofvarious ages and also from chickens of various developmental
stages.

Division of Developmental Biology
Professor: lkuo Takeuchi
Associate Professor: Masaki lwabuchi
Research Associate: Masao Tasaka
This division is devoted to the study of regulation in development,using the developmental system of cellular slime molds. The
development of these organisms is characteristic in that after tissue
formation, two types of cells. called prestalk and prespore cells.
differentiate in a fixed proportion at the anterior and posterior partsof the tissue respectively and that removal of a portion of thetissue brings about cell-type conversion between the two presumptive cells and results in regeneration of the normal differentiation
pattern without accompanying cell division . To elucidate the regulatory mechanisms of gene expression during the processes of celldifferentiation, we have isolated 2 and 6 eDNA clones for mRNA
which specifically accumulate in prestalk and prespore cells.
respectively. Analyses using eDNA clones indicated that both cell
types begin to differentiate around the same stage in development.
accompanying changes in transcription of a set of cell-type specificgenes. Further analyses along this line were pursued during 1987,

as follows:
I. Prespore specific gene expressiona) Gene encoding a spore coat protein
By screening the eDNA libraries in 'J. gt11 with a polyclonal antibody
against Dictyostelium discoideum spores, we have obtained a eDNA
clone encoding a spore coat protein. The Northern blotting analyses
showed that the mANA of ca. 2.2kb first appeared at the tipped
aggregate stage and accumulated in prespore and spore cells at thelater stages of development. The Southern blotting analyses
indicated that the gene is unique. The genomic fragments covering
the coding, 5'upstream and 3'downstream regions were isolated.
The gene includes one open reading frame separated by one intron
and encodes the peptide composed of 595 amino acids(Mr.59158.27). This peptide has a leader sequence at the Nterminal, one possible N-glycosylation site in the amino-half andthree independent repeated amino acids sequences. We detected a
few DNase-1 hypersensitive sites in the 5'upstream region characteristic of the expressed gene.
b) Gene encoding elongation factor 2
Full length eDNA clones hybridized with the hamster elongation
factor 2 (EF2) eDNA were isolated from the libraries. The completeamino acid sequence of D. discoideum EF2 deduced from thenucleotide sequence of cDNAs indicated more than 60% overall
homology with the hamster EF2, the homology being as high as ca.
80% in its GTP- and ribosome-binding regions. Its molecular weight
was estimated 91 ,699 and the site of ADP-ribosylation by diphtheria
toxin was identified. The Southern blotting indicated that the geneencoding the EF2 is unique. We have isolated the genomic DNA
clones including the coding, 5'wpstream, and 3'downstream regions.
The Northern blotting analyses indicated that the expression of themANA was highest during the vegetative stage and gradually
decreased through development. As prestalk cells differentiated, themANA disappeared in these cells, thus becoming highly enriched inprespore cells compared to prestalk cells. Whether this was due to

14

specific turning-off of transcription of this gene or degradation of
the mRNA in prestalk cells is now being examined.
II. Prestalk specific gene expression
By the use of a prestalk specific eDNA clone, the genomic DNA
fragment, including the coding 5'upstream and 3'downstream
regions, was isolated and sequenced. We have obtained an
antibody produced against the fusion protein encoded by this gene.
The antibody recognized a protein of Mr45,000. Expression of thisgene in cells and tissues as well as the nucleotide sequences
involved in regulation of its expression are now being examined .

DEPARTMENT
OF REGULATION BIOLOGY
Chairman: Goro Eguchi (adjunct)
Two divisions and two adjunct divisions belong to the department.
and conduct research on information processing and control
mechanisms in biological systems .

Division of Sensory Processing
Professor: Ken-lchi Naka
Associate Professor: Shozo Vasui
Research Associate: Hiroko Sakai
Eiki Hida
NEURON NETWORK IN THE VERTEBRATE RETINA
The goal of our study is t0 define how signals are processed ina neuron network. The network we have chosen is that in thevertebrate retina because : 1) the retina is essentially a twodimensional structure of a few hundred microns in thickness, 2) it

is distinctly layered and constituent neurons have a characteristic
morphology and physiology, 3) the input-output relationships are
well-defined, and 4) neurons within can be reached from outside
without much difficulty. These factors make the vertebrate retina an'ideal model' to unravel the working of a neuron network. In
essence the retina is an approachable part of the brain. From thenature of our subject our research is interdisciplinary and isdistinctly different from the other types of 'biological' researchwhich are mostly matter oriented. To achieve our goal we havemade two original choices in methodology and preparation.
One of the most basic approaches in science and engineering is tostimulate (or to excite) a system and to observe its response. This
is to identify a system by observing how it responds to a certain
input: a black-box approach. Norbert Wiener suggested the use ofGaussian white-noise as test signal and to identify a systemthrough a series of orthogonal functionals known as the Wiener
G-functionals or kernels . Gaussian white-noise is a formal derivativeof the fundamental chaos. Brownian motion, a concept examinedrigorously in mathematics.
Twenty years ago, we began our pioneering effort to apply thetheory to identify a practical system. the neuron network in theretina, and our past effort has made it a legitimate means inneurophysiology. This is testified by the fact that Dr H. M. Sakai
has lately been invited to contribute a chapter. "White-noise
Analysis in Neurophysiology", to the prestigious Annual Review ofPhysiology. In addition to the esthetic appeal of the theory,
Wiener's methodology has many practical advantages over the
traditional testing of systems by sinusoidal or pulsatile inputs. It is atribute to the great mathematician that he formalized the theory in
his late fifties. During the course of our application effort, we havedeveloped a comprehensive software system to execute analysis
efficiently and effectively as prescribed by Wiener's theoreticalframework.
In biology, the choice of preparation is often as important as the

16

choice of methodology. The preparation must be original and should
offer the best chance of achieving the research objective. We havechosen the retina of channel catfish, lctalurus punctatus, as the
subject of research. The retina is now the most thoroughly studiedvertebrate retina, and our choice made twenty years ago has been
vindicated. N. Osborne and J. Chader wrote on catfish research:"This is not only due to improvements in technology but to thebrilliance and dedication of workers in the field who have been able
to put together the functional circuitry of the retina in relation to its
morphology".
Our research has produced several salient results. In morphology,
we have discovered three new synapses: the synapse made by thehorizontal cells back to the receptors, the synapse made by thehorizontal cells onto the amacrine cells and the synapse made by
the ganglion-cell dendrites. Functional evidence shows that thesynapse is functional. In view of the fact that retinal ganglion cells
are the most extensively examined neurons in the central nervous
system. this discovery is noteworthy.
In the functional domain, we have identified the dynamics of retinal
neurons to show that there are two principal transformations in theretina: the piecewise linearization in the outer retina and thegeneration of static (second-order) nonlinearity in the amacrine cells.
These linear and non-linear components are encoded into spiketrains. a point process. to be sent to the brain. Cross-correlation
between white-noise inputs and resulting spike discharges recovers
the linear and nonlinear components. Thus the neuron network in
the retina can be approximated by the Wiener/Korenberg cascade
structure.
We have also discovered that neurons in the inner retina areextensively coupled by fast and bi-directional pathways and suchinteractions are linear. Groups of tightly inter-connected neurons areloosely coupled to each other through a band-pass filter. Thus, thenotion of central interaction through either excitatory or inhibitory
synapses needs to be modified in the retinal neuron network.

Division of Cellular Regulation
Professor: Norio Murata
Associate Professor: Hideaki Nakashima
Research Associate: Takao Kondo
lkuo Nishida
This division studies two important phenomena in higher plants andmicroalgae : (1) The molecular mechanisms of low-temperaturesensitivity and adaptation to temperature stress. (2) The structureand function of chloroplast membrane proteins in photosynthesis.
The temperature work emphasizes the participation of biologicalmembranes and membrane lipids: ( i) An enzyme (glycerol-3phosphate acyltransferase) has been isolated which produces
special lipid molecules that are responsible for the low-temperaturesensitivity of higher plants, and its eDNA has been cloned . Studies
aiming at the plant transformation in the low-temperature sensitivity
by the cloned DNA are taking place. (ii) Adaptation of plants totemperature stress is studied using tobacco culture cells andcyanobacteria. These organisms increase the degree of unsaturationof membrane lipids when exposed to low temperature. Enzymes
which catalyze the desaturation of fatty acids are under investigation .
Photosynthesis research is focused on the machinery of theoxygen-evolving complex. This complex is embedded in chloroplast
thylakoid membranes and consists of about 20 kinds of protein
components. In order to elucidate the molecular mechanism of theoxygen evolution. the following subjects are presently studied bytechniques of biochemistry, physicochemistry and molecular biology: ( i) Purification and property of the structural components ofthe complex. (ii) Three-dimensional interaction of the protein
components within the complex. (iii) The functional role of eachcomponent in oxygen evolution.

Division of Control of Gene Regulation
This division was newly opened in 1988. Its chief intention is to
study the control mechanisms of gene expression in multicellularorganization. with particular attention to the introduction of technology of transgenesis of animals and plants. Research activities are
expected to start from the later half of the fiscal year of 1988.

Division of Biological Regulation (Adjunct)
Professor: Hidemasa lmaseki
Associate Professor: Mikio Nishimura
Research Associate: Kotaro Yamamoto
Satoru Tokutomi
Wataru Mitsuhashi
Higher plants always regulate their growth rate and differentiation
processes according to their environmental conditions such as light.
temperature. water availability and so on. Under environmental
stimuli. changes in cellular functions take place in plant cells long
before any changes are detected morphologically. The Division of
Biological Regulation conducts research at the biochemical andmolecular biological level on the controlling process of higher plants
triggered by changes in environmental · condition.
The research programs being carried out at present are (1) study of
the rapid growth and opening of plumules during germination ofmung bean (Vigna radiata) seeds. (2) study of the acquisition ofthermotolerance in mung bean seedlings, and (3) study of thestructure-function relationship of phytochrome. a photoreceptor
which senses light as environmental stimuli.
(1) Biochemical and molecular biological study on rapid growth ofplumules : During germination of a mung bean seed, the plumule
starts to grow and open after imbibition . The growth and opening is
mainly due to the enlargement of cells. Since the process requi Fes
synthesis and accumulation of large amounts of carbon and nitrogencompounds. sucrose and amide metabolism must be activated prior17

to it. The aim of this study is to clarify the molecular mechanism ofactivation of the metabolism, which is triggered by imbibition.
At present, we are studying the role of invertase and sucrosesynthase in carbon metabolism. Activities of these enzymes
increase dramatically two days after imbibition. As for nitrogenmetabolism, we are investigating the role of asparaginase since
asparagine comprises 40 % of nitrogen compounds supplied to aplumule from the other parts of the seedling. We are using
immunochemical methods to test whether the increase of enzyme
activity results from the activation of inactivated enzymes or fromthe de novo expression of their genes.
(2) Mechanism of the aquisition of thermotolerance : When plantseedlings are exposed to high temperature (ca. 50°C) for about 20
min, they are irreversibly damaged and die, even if they are
returned to a normal growth temperature (ca. 28°C) again. However,pretreatment at medium temperature (ca. 40°C) provides protection(thermal tolerance) to a subsequent exposure to the high temperature. The acquisition of thermotolerance has been observed universally in organisms. Some changes in the structure and function of
biological membranes may be involved in thermotolerance, as isobserved in thermal adaptation. To discover the mechanisms ofaquisition of thermotolerance, investigations on the involvement of
biological membranes in the phenomena have been started. The
effect of these thermal treatments on the physicochemical properties, such as fluidity, permeability and surface potential has beenstudied in total lipid membranes.
(3) Structure-function study of phytochrome: Phytochrome is amajor photoreceptor of photomorphogenic responses in plants. It
absorbs light and transmits a signal to other cellular components inan unknown manner. In order to uncover the primary reaction ofphytochrome. this study aims to identify functional domains ofphytochrome with respect to light absorption and conformationalchanges which subsequently take place. At the moment, we areinvestigating the macromolecular structure of phytochrome by small

18

angle X-ray scattering, and characterizing a functinal domain for lightabsorption by the methods of protein chemistry and spectroscopy.

Division of Behavior and Neurobiology(Adjunct)
Professor: Katsuhiko Mikoshiba
Associate Professor: Masaharu Ogawa
Research Associate: Takaaki Tamura
In our research division, we are studying the mechanism ofdevelopment, growth, and differentiation of the mammalian nervous
system at the molecular level. The main themes of our division are:(1) Studies on myelin-deficient mutant animals. We have produced
several mutants with abnormalities at different steps of myelinogenesis. We are studying myelination as a typical morphogenetic
phenomenon in the central nervous system, and also as aneuron-glia inter-relationship.
(2) Studies on the mechanism of brain specific gene expression.
We are studying these subjects with both in vitro and in vivo
systems. In the in vivo system. we are studying promoter activityof myelin basic protein (MBP). proteolipid protein (PLP) and JC virus
genes by brain-specific in vitro transcription system.
(3) Studies on the structure and function of brain-specific proteins.
Brain-specific proteins such as P400 , which is specifically expressedin Purkinje cells in the cerebellum. Antisera and eDNA clones of theP4oo have been obtained. We are studying the structure of themRNA and the genomic DNA of the P400 as well as protein
structure and its functions.

TECHNICAL DEPARTMENT
Chief: Hachiro Honda
The Technical Department is a supporting organization for researchers and research organizations within the INIBB. The department
develops and promotes the institute's research activities and, at thesame time, maintains the research functions of the institute.
The Department is organized into two groups: one, the Common
Facility group, which supports and maintains the institute's common
research facilities and the other, the Research Support group, whichassists the research activities.
Technicians participate, through the department. in mutual enlightenment and education to increase their capability in technical areas.
Of 26 staff members 14 belong to the Common facility group(Chief; Hiroyuki Hattori). whereas the other 12 are supporting
research at each division.

RESEARCH RESOURCE
There are four categories of research support facilities available toresearch members am:! visiting biological scientists: 1) Campus-wide
facilities (CENTRAL COMPUTING CENTER and LIBRARY), 2) NIBS's
own research support facilities (RESEARCH SUPPORT FACILITY,
intramural). 3) facilities jointly maintained by the NIBB and the ·IPS
but managed either by the NIBB (RADIOISOTOPE FACILITY), or by
the IPS (ANIMAL-CARE FACILITY). 4) facilities jointly run by NIBB
and IPS (CENTER for ANALYTICAL INSTRUMENTS, ELECTRONMICROSCOPE CENTER, LABORATORY GLASSWARE FACILITY,
MACHINE SHOP AND LOW-TEMPERATURE FACILITY).

RESEARCH SUPPORT FACILITY
Head of Facility: Yoshitaka Nagahama
Faculty: Masakatsu Watanabe (Spectrograph)
Yoshio Hamada (Tissue and Cell Culture)
The facility maintains large-scale experimental equipment andfacilities for growing and maintaining biological specimens. The
facility is shared among the research members, and has fivelaboratories.
The Large Spectrograph Laboratory: This laboratory has the largest
spectrograph in the world dedicated mainly to action spectroscopicalstudies of various light-controlled biological processes (the Okazaki
Large Spectrograph, OLS). The spectrograph runs on a 30KW-Xenon
arc lamp and has a compound grating-surface composed of 36
smaller individual gratings. It projects a spectrum of a wavelength
range from 250 nm to 1,000 nm onto its focal curve of 10 m inlength. The fluence rate (intensity) of the monochromatic light ismore than twice as much as t~at of the tropical sunlight at noon ateach wavelength. More than 30 projects every year are conductedby visiting scientists including foreign scientists as well as those in
the Institute under the NIBB Cooperative Research Program for theuse of the OLS.
Tissue and Cell Culture Laboratory: This is a facility for tissue andcell culture. This laboratory is equipped with safety rooms which
satisfy the P3 physical containment level. This facility is routinely
used for DNA recombination experiments.
Laboratory Computer Facility: The NIBS's computing is handled bya network of Digital Equipment Corporation's VAX 11/780 and VAX
2,000 with Floating Point System's AP120B and 5310 arrayprocessors. An Ethernet interfaces the network with a number oflaboratory PC's. An extensive software system for time-series
analysis developed in-house has been operating for the last 8 years.

19

A limited number of image processing routines are also available.
Plant Culture Facility: There are a large number of culture boxes,
cubicles, and a limited number of rooms With environmental control
for plant culture.
Experimental Farm: This facility consists of two 20 m 2 glass-houses
with precision temperature and humidity control. a limited farm, twogreen houses (45 m2 , 88 m 2 ) with automatic sprinklers and window
control. two open aquariums (30 t., 50 t.) and several smaller tanks .
The facility also includes a building with office, storage andwork-space.
Plant Cell Culture Facility: Autotrophic and heterotrophic culture
devices are equipped for experimental cultures of plant andmicrobial cells .
The faculty of the Large Spectrograph Laboratory conducts its own
work. Photoreceptive and signal-transduction mechanisms of phototaxis of single-celled. flagellate algae are studied actionspectroscopically by measuring computerized-videomicrographicallythe motile behaviors of the cells at the individual cell level and at
the subcellular level. Photoreceptive and signal transduction
mechanisms of algal gametogenesis are also studied actionspectroscopically.
The faculty of the Tissue and Cell Culture Laboratory also conducts
its own work. Myosin is a ubiquitous protein in eukaryotes. It is
presumed that this molecule plays a key role in cell motility as wellas other functions . The role of myosin in non-muscle cells might bebetter understood if wild-type cells could be compared withmutants cells with altered myosin molecules. though such mutants
have not been isolated. We intend to examine the function of the
myosin heavy chain in vertebrate non-muscle cells by interrupting
its synthesis with the introduction of antisense RNA molecules.

20

RESEARCH FACILITIES RUNJOINTLV WITH IPSRADIOISOTOPE FACILITIES(managed by NIBB)Head of Facility: Yoshihiko Fujita
Faculty: Kohji Hasunuma
The facility is composed of a center and two subcenters, one in
NIBB and the other in IPS. The facility is being used for molecularanaylses of eukaryotes. At the center. a variety of radioisotopes
such as 3 H, 14C. 22 Na. 32 P, 35 S, 45 Ca and 125 1 are handled. as wellas various species of beta and gamma-ray emitting nucleides. A
P3-level laboratory for recombinant DNA research is included in thecenter facilities. At the substations. only a limited variety ofradioisotopes such as 3 H. 14C and 32 P are processed. The
substation in NIBB is equipped with a P2-level recombinant DNA
research laboratory. The members of the Radioisotope Facility
maintain and control the center. and give users appropriate
guidance for radioisotope handling .
The faculty of the facility conducts its own research on the signaltransduction chain of light to cycle nucleotides in Neurospora crassa
and Lemna paucicostata. Several species of ATP-GTP-binding proteins were ADP-ribosylated by endogenous ADP-ribosyltransferase.
The goal of the research is to isolate the genes for ATP-GTP-binding
proteins and ADP-ribosyltransferase and to analyze how the external
singals such as light and hormones were transduced to theintracellular second messengers.

CENTER FOR ANALYTICAL INSTRUMENTS(managed by NIBB)Head of Facility: Norio Murata
The Center for Analytical Instruments consists of the following fivesections. (1) Chemical analysis, (2) Preparation of Biological Materials, (3) Spectroscopic analysis, (4) Physical analysis, and (5)Microscopic analysis. Each section is equipped with instruments forgeneral use as listed below.
1. Section for Chemical Analysis
Amino Acid Analyzer
Gas Chromatograph
HPLC
Protein Sequence Analyzer
Peptide Synthesizer

HITACHI 835
SHIMAZU GC-7APTF
JASCO TRIROTAR Ill
JEOL JAS-47K
BECKMAN 990C

2. Section for Preparation of Biological Materials
Coulter Counter
COULTER ZB
lsotachophoresis System
LKB 2127 TACHOPHOR
Preparative Ultracentrifuge
BECKMAN L8-80
Two Parameter Cell Sorter
BECTON-DICKINSON FACS-11
3. Section for Spectroscopic Analysis
Atomic Absorption
Spectrophotometer
PERKIN-ELMER 603
Differential Refractometer
CHROMATIX KMX-16
Dual-wavelength
Spectrophotometer
HITACHI 557
Infrared Spectrophotometer
JASCO A-302
Laser-Raman Spectrophotometer JASCO R-800
CHROMATIX KMX-6DC
Light Scattering Photometer
Spectrofluorometer
HITACHI MPF-4

22Spectrophotometer
Spectropolarimeter

GILFORD 250
HITACHI 330
JASCO J-40S

4. Section for Physical Analysis
Analytical Ultracentrifuge
HITACHI 282
Differential Scanning Calorimeter PERKIN-ELMER DSC-2
EPR Spectrometer
BRUKER ER 200D
GC Mass Spectrometer
HITACHI M-80
GC/LC Mass Spectrometer
JEOL DX-300
Superconductive FT-NMR
Spectrometer
BRUKER WM 360wb
CONTRAVES RM-30
Viscometer
5. Section for Microscopic Analysis
2-Dimension M icrodensitometer JOYCE LOEBL 3CS
Film Data Analysis System
NAC MOVIAS GP-2000
Image Analyzer
KONTRON MOP-AM03
KONTRON IBAS-I & II
Interactive Image Analyzer
Microscope Photometer
CARL ZEISS MPM 03-FL
ANIMAL-CARE FACILITIES(managed by IPS)

Vivarium : This is a 2,1 00 m2 building for maintaining land animals,
including insects. Operations and experiments of a limited scope
may be performed in the vivarium.
Aquarium : This is a 600 m2 facility for both fresh- and sea-water
animals. There are twelve 1-ton and fourty 0.5-ton tanks in addition
to one 7-ton and one 2-ton circular tanks. All tanks are individuallytemperature controlled and are supplied either with deionized wateror seawater. There is a lorry with a 2.2-ton temperature-controlledtank to transport aquatic animals and plants.

ELECTRONMICROSCOPE CENTER(managed by IPS)This facility maintains the following microscopes for the use lt>y theinstitute's members as well as researchers from other universities
and research institutions.
Transmission microscope: Hitachi H-500 125KV, JOEL 100-CX and
200-CX 100 and 200KV and Philips EM-400HM 120KV.
Transmission scope, analytical: JOEL 200-CX, 200KV.
Scanning scope : Hitachi S-450 25KV.

23

COOPERATIVE RESEARCH ACTIVITIESThe NIBB sponsors four cooperative research activities.
Individual and group cooperative research program: Scientists from other Japanese universities andresearch institutes are invited to undertake joint research projects with the institute's members.
Limited funds are available for travel and expenditures related to the projects.
Research conferences : The NIBB sponsors research conference on important subjects in biology.
Ten to twenty scientists are invited and intense discussion is held for two to three days. The NIBB
provides financial support for the participants. Conferences may be initiated by the institute's
members or scientists from other institutes.
Facility-sharing program: Scientists with other universities are permitted to use specified
instruments maintained by the NIBB. No financial support is available except for the Large Scale
Spectrograph whose user is provided with limited financial support.
Graduate student programs: Graduate students with other universities may spend a fixed period oftime with members of NIBB. This allows students to have experience with the very modern
facilities of NIBB. The NIBB is expected to have its own graduate program in the near future.
Bioscience training course: Since 1986, the NIBB has organized an advanced course with practical
exercises on a special subject on the fore-front of the biological sciences. The course is held
annualy with about 12-20 attendants above the graduate student level from other Japanese
universities and research organization (both national and private).

24

LIBRARY
The three institues of the Okazaki National Research Institutes share a common library facility. The
library is a part of the main administration building and has a floor space of 2.500 m 2 .
The library has 9,350 books in Japanese and 30,050 in foreign languages. and subscribes to 237
Japanese and 446 foreign journals. Lending records. inventory and literature searches arecomputerized . The library is open 24 hours a day.

LODGING FACILITYThe Okazaki National Research Institutes maintain two lodging facilities, the Mishima Lodge andYamate Lodge.
Mishima Lodge is a few minute walk away; Yamate Lodge is less than 20 minutes. The lodges arefor scientists and their families staying for fixed periods of time with the institutes. Some suites
and bungalows are provided with kitchenette facilities . There is a modest charge to help maintainthe facilities .
Mishima Lodge has 54 single rooms, 4 twin rooms. 6 suites, and 10 bungalows for large families.
Yamate Lodge has 11 single rooms, 4 suites and 2 family complexes .
On the campus there is a dining facility which is open Monday through Saturday.

25

THE CITY
The city of Okazaki, incorporated in 1916, is located 30 kilometers
southwest of Nagoya, the fourth largest city in Japan. A high-speed
urban train connects the two cities. Okazaki, with a population of
275,000, is a typical medium-size city in Japan and offers theconvenience of urban life while avoiding the disadvantages of alarge city. Okazaki is the commercial as well as cultural center ofthe Mikawa (Three River) district with its rich historical heritage.
lyeyasu Tokugawa, the first Tokugawa Shogun, was born here in
1542 and built a castle here. The original parapets and moats andthe rebuilt castle still dominate the city as they did 500 years ago.
When he established the Shogunate in Edo (the former name ofTokyo) in 1603, lyeyasu took a large contingent of Mikawa Bushi(Mikawa Samurai or professional warriors) with him . Those Mikawa
Bushi formed the nucleus of lyeyasu's new administration.
Within a radius of 10 kilometers from the city center are locatedMitsubishi Motor's Okazaki Plant. Toyota Motor's main production
facilities in Toyota city, and Sony's ultramodern Koda plant which
produces video tape/tape recorders.

From Toycrhashi to Okazaki : Twenty five minutes by the Mei-tetsu(Nagoya Railway) express. Mei-tetsu's station in Okazaki is Higashi(or East)-Okazaki. The train runs every 20 to 30 min. This is themost convenient route to come to Okazaki from Tokyo.
From Tokyo to Nagoya: Two hrs by the JR's super-express train(HIKARI or LIGHTENING). The train runs every 30 min.
From Kyoto/Osaka to Nagoya: One to two hrs by the JR's
super-express train (HIKARI/KODAMA). Kin-tetsu (Kinki Nippon Railway) also serves Osaka/Nara and Nagoya.
From Nagoya to Okazaki: Thirty five minutes by the Mei-tetsu
express which runs every 20 min.
In March 1988, New Station, Mikawa-Anjyo, was opened for thesuper-express train (KODAMA). About 45 minutes from there to
Okazaki by taxi.

BY AIR
Domestic airlines serve Komaki, Nagoya, airport which is 1 hour
drive from Okazaki. Limosine service began in July 1988 between
Nagoya airport and Okazaki.

TRANSPORTATIONBY TRAIN
From Tokyo to Toyo-hashi: Two and half hours by the Japan
Railway's (JR-Tokai) super-express train (KODAMA or ECHO). The
train runs every 30 min.

26

WEATHERIn Okazaki, temperatures reach 30 degrees centigrade in thesummer and go down to a few degrees above freezing in thewinter. May to June is the rainy season and the spring (March toMay) and fall (September to November) are the most pleasant.

THE OKAZAKI CASTLE,
THE HOME OF THE FIRSTTOKUGAWA SHOGUN, IYEYASU.

27

OFFICE OF DIRECTOROKADA, T.S. (1986).
Introduction : Can specialized cells change their phenotypes? Curr. Top Develop. Bioi. (Moscona, A.A. a d Monroy, A. eds.)
20 . In "Committment and Instability in Cell Differentiation". (Okada, T.S. and Kondoh, H. vol. eds.). pp.XV-XX. Academic Press.

Dual regulation of expression of exogenous crystallin gene in mammalian cells: A search for molecular
background of instability in differentiation. Curr. Top. Develop. Bioi. (Moscona, A.A. and Monroy, A. eds.) 20. In "Committment andInstability in Cell Differentiation". (Okada, T.S. and Kondoh, H. vol. eds.). pp.153-164, Academic Press .

KoNDOH, H. and OKADA, T.S. (1986).

OKADA, T.S. (1986).

Transdifferentiation in animal cells: Fact or artifact? Develop. Growth and Differ. 28, 213-221.

KoNDOH, H., HAYASHI, S., TAKAHASHI, Y., and OKADA, T.S. (1986).
Regulation of 6-crystallin expression: an investigation by transfer of thechicken genome into mouse cells. Cell Differ. 19. 151-160 (1986).
UEDA, Y. and OKADA, T.S. (1986).
19, 179-187.

Transient expression of a "lens-specific" gene. b-crystallin, in embryonic chicken adenohypophysis. Cell Differ.

0ZATO, K., KONDOH, H., INOHARA, H., IWAMATSU, T., WAKAMATSU, Y., and OKADA, T.S. (1986).
expression of chicken b-crystallin gene in embryos. Cell Differ. 19, 237-244.

Production of transgenic fish: introduction andExpression of the endogenous chicken 6-crystallin genes incorporated in mouse
teratocarcinoma cells. In "Coordinated Regulation of Gene Expression". (Clayton. R.M . and Truman, D.E.S. eds.). pp.183-191, Plenum
Pub. Co., London.

OKADA, T.S., TAKAHASHI, Y., and KoNDOH, H. (1986).

Tissue-specific expression of cloned chicken crystallin genes inmammalian cells. In "Coordinated Regulation of Gene Expression". (Clayton, R.M. and Truman, D.E.S. eds.). pp.143-147, Plenum Publ.
Co .. London.

KoNDOH, H., HAYASHI, S., OKAZAKI, K., YASUDA, K., and OKADA, T.S. (1986).

Structure and expression of the chicken crystallin genes. In "Oxford Surveys of Eukaryotic Genes". Vol.3(Mclean, N. ed.), pp.183-209, Oxford University Press, Oxford.

YAsUDA, K. and OKADA, T.S. (1986).

KONDOH, H., U~DA, Y., HAYASHI, 5., OKAZAKI, K., YASUDA, K. and OKADA, T.S. (1987).

An attempt to assay the state of determination by usingtransfected genes as probes in transdifferentiation of neural retina into lens. Cell Differ. 20, 203-207.

28

KONDOH, H., I<ATOH, K., NOMURA, V., FUJISAWA, H., YOKOYAMA, M., KIMURA, S., I<ATSUKI, M., SAITO, M., NOMURA, T., HIRAMO'TO, V., and OKADA,
T.S. (1987).
Specific expression of the chicken o-crystallin gene in the lens and the pyramidal neuFons of the piriform cortex intransgenic mice. Develop. Bioi. 120. 177-185.
Expression of the gene coding for lens-specific crystallins in the nervous tissues. In "Strategy and Prospects in
Neuroscience". (Hayashi, 0. ed.), pp.151-160, Japan Scientific Societies Press. Tokyo.

OKADA, T.S. (1987).

HAYASHI, S., GoTO, K., OKADA, T.S. and KONDOH, H. (1987).

Lens-specific enhancer in the third intron regulates expression of the chicken

o-crystallin gene. Genes and Develop. 1, 818-828.

DEPARTMENT OF CELL BIOLOGYDIVISION OF CELL MECHANISMS
Preferential digestion of male chloroplast nuclei and mitochondrial nuclei during gametogenesis of Bryopsis
maxima Okamura. Protoplasma 133, 443-453.

KUROIWA, T. and HORI, T. (1986).

KUROIWA, T., MIYAMURA, S., KAWANO, S., HIZUME, M., THO-E, A., MIYAKAWA, I. and SANDO, N. (1986).
bivalent of Saccharomyces cerevisiae. Exp. Cell Res. 165, 199-206.

Cytological characterization of NOR in the

KUROIWA, 1'. and NAKAMURA, S. (1986).

No effect of preferential digestion of chloroplast genome of male origin on chloroplast genome offemale origin in young zygotes of Chlamydomonas reinhardtii as revealed by a video-intensified microscope photon counting system .
Acta Histochem. Cytochem. 19, 95-102.

MITA, T., KANBE, T., TANAKA, K. and KUROIWA, T. (1986).

A ring structure around the dividing plane of the Cyanidium caldarium chloroplast.

Protoplasma 130, 212-213.
MIYAMURA, S., NAGATA, T. and KUROIWA, T. (1986).

Quantitative fluorescence microscopy on dynamic changes of plastid nucleoids during wheat

development. Protoplasma 133, 55-72.
Behavior of chloroplast nucleus during chloroplast development and degeneration in
Chlamydomonas reinhardii. Plant Cell Physiol. 27. 775-784.

NAKAMURA, S., ITOH, S. and KUROIWA, T. (1986).

29

OGAWA, K. and KUROIWA, T. (1986).
701-710.

Base-specific endo-exonucleolytic activity of Chlamydomonas nuclease C1 & 2. Plant Cell Physiol. 27,

OKADA, K., NAGATA, T. and TAKEBE, I. (1986).
619-626.

Introduction of functional RNA into plant protoplasts by electroporation. Plant Cell Physiol. 27,

OKADA, K., TAKEBE, I. and NAGATA, T. (1986).
Gen. Genet. 205, 398-403.

Expression and integration of genes introduced into highly synchronized plant protoplasts. Mol.

SUZUKI, T., KANBE, T., KUROIWA, T. and TANAKA, K. (1986).
Gen. Microbial. 132, 443-453.

Occurrence of ploidy shift in a strain of the imperfect yeast Candida albicans. J.

KAMATA, Y. and NAGATA, T. (1987).
Enrichment of heterokaryocytes between mesophyll and epidermis protoplasts by density gradientcentrifugation after electric fusion . Theor. Appl. Genet. 75, 26-29.
KAWANO, S., ANDERSON, R.W., NANBA, T. and KUROIWA, T. (1987).
polycephalum . J. Gen . Microbial. 133, 3175-3182.

Polymorphism and uniparental inheritance of mitochondrial DNA in Physarum

A third multiallelic mating-type locus in Physarum polycephalum . J. Gen. Microbial. 133.

KAWANO, S., KUROIWA, T. and ANDERSON, R.W. (1987).
2539-2546.

MIYAKAWA, I., SANDO, N., KAWANO, S., NAKAMURA, S. and KUROIWA, T. (1987).
the yeast Saccharomyces cerevisiae. J. Cell Sci . 88, 431-439.

Isolation of morphologically intact mitochondrial nucleoids from

MtYAMURA, S., KUROIWA, T. and NAGATA, T. (1987).
Disappearance of plastid and mitochondrial nucleoids during the formation of generative
cells of higher plants revealed by fluorescence microscopy. Protoplasma 141, 149-159.
NAGASHIMA, H., KUROIWA, T. and FUKUDA, I. (1987).
28, 315-321.

Chloroplast nucleoids during greening of Cyanidium caldarium M-8 type. Plant Cell Physiol.

NAGATA, T. (1987).

Interaction of plant protoplast and liposome. Method . in Enzymol. 148, 34-39.

NAGATA, T. (1987).

Liposome entrapment for delivery of genetic material to plant protoplasts. Method. in Enzymol. 149, 176-184.

NAGATA, T., OKADA, K., KAWAZU, T. and TAKEBE, I. (1987).
cells. Mol. Gen. Genet. 207, 242-244.

30

Cauliflower mosaic virus 35S promoter directs S phase specific expression in plant

NAKAMURA, S., OGAWA, K. and KUROIWA, T. (1987).
NEMOTO, Y., KuROIWA, T. and FURUYA, M. (1987).
Physiol. 28, 1043-1 049.

Survey of Ca 2 + -dependent nuclease in green plants. Plant Cell Physiol. 28, 545-548.
Photocontrol of nuclear DNA replication in Chattonella antiqua (Raphidophyceae). Plant Cell

Light and electron microscopic observations of mitochondrial fusion in plasmodia induced
sporulation in Physarum po/ycephalum. Cytologia 52, 599-614.

NISHIBAVASHI, S., KAWANO, S. and KUROIWA, T. (1987).

Melanoma dynein : evidence
that dynein is a general "motor" for microtubule-associated cell motilities. Eur. J . Cell Bioi. 43, 3-9.

OGAWA, K., HOSOVA, H., YOKOTA, T., KOBAYASHI, T., WAKAMATSU, Y., 0ZATO, K., NEGISHI, S. and OBITA, M. (1987).

Preferential resistance of phosphodiester bonds between deoxycytidine and 5'-adjacent bases toChlamydomonas nuclease C. Plant Cell Physiol. 28, 323-332.

OGAWA, K. and KUROIWA, T. (1987).

DIVISION OF BIOENERGETICSFALKOWSKI, P.G., FUJITA, V., LEV, A. and MAUZERALL, D. (1986).
pyrenoidosa. Plant Physiol. 81, 310-312.

Evidence for cyclic electron flow around photosystem II in Cholorella

Characterization by EPA Spectroscopy of cytochrome b-562 isolated from thecytochrome b-c 1 complex of R. sphaeroides . J . Biochem. 100, 1477-1480.

IBA, K., TAKAMIVA, K., NISHIMURA, M. and ITOH, S. (1986).

Chlorophyll organization in p700-enriched particles isolated from spinach chloroplasts. CD and absorption
spectroscopy. Biochim . Biophys. Acta 851, 75-85.

IKEGAMI, I. and ITOH, S. (1986).

Characteristics of the Cl - action site in 0 2 evolving reaction in PS II Particles : electrostatic interaction with ions.
Plant Cell Physiol. 27, 25-36.

ITOH, S. and UWANO, S. (1986).

Rate of exchange of Cl - between the aqueous phase and its action site in the 02 evolving reaction ofphotosystem II studied by rapid ionic-jump-induced Cl- depletion. FEBS Let. 195, 140-144.

ITOH, S. and IWAKI, M. (1986).

ITOH,

S. (1986).

Change of surface potential and intramembrane electrical field induced by the movements of hydrophobic ions insidechromatophore membranes of Rhodopseudomonas sphaeroides studied by responses of merocyanine dye and intrinsic carotenoids. In"lon Interactions in Energy Transducing Biomembranes" . (Papageorgious, G.C., Baber. J. and Papa, S. eds.) pp.75-86, Plenum Press.
New York.

31

ITOH, S.

and NISHIMURA, M. (1986).

Rate of redox reactions related to surface potential and other surface-related parameters in biologicalmembranes. In "Methods in Enzymology" . Vol.125, pp.58-86 Academic Press, New York.

ITOH, S., TANG, X.-S. and SATOH, K. (1986).

Interaction of the high-spin Fe atom in the photosystem II reaction center with the quinones QA
and 0 6 in purified oxygen-evolving PS II reaction center complex and in PS II particles. FEBS Let. 205, 275-281 .

and ITOH, S. (1986).
Electrical potential changes in the surface and the central region of chromatophore
membranes of photoysnthetic bacteria detected by the absorbance changes of ethidium and carotenoid. Biochim. Biophys. Acta 849,
141-149.

MASAMOTO, K., MATSUURA, K.

MATSUURA, K. and FUJITA, Y. (1986).

Chromatic regulation of cytochrome composition and electron transfer from cytochrome b6 -f complex tophotosystem I in Anacystis nidulans (Tx 20). Plant Cell Physiol. 27, 685-691.

MURATA, N., ARAKI, S., FUJITA, V., SUZUKI, K., KUWABARA, T. and MATHIS, P. (1986).

Stoichiometric determination of pheophytin in photosystem

II of oxygenic photosynthesis. Photosyn. Res. 9, 63-70.
NAKAMURA, S., ITOH, S. and KUROIWA, T. (1986).

Cultures of the pelagic cyanophytes Trichodesmium erythraeum and T. thiebautii in synthetic

medium. Marine Biology 91, 9-13.
SUZUKI, R. and FUJITA, Y. (1986).

Marine Ecology -Chlorophyll decomposition in Skeletonema costatum: a problem in chlorophyll determination of water samples.
Progress Series 28, 81-85.

TAKAHASHI, M., ISHIZUKA, J., ISHIMARU, T., ATKINSON, l.P., LEE, T.N., YAMAGUCHI, V., FUJITA, V. and ICHIMURA, S. (1986).

Temporal change innutrient concentrations and phytoplankton biomass in short time scale local upwelling around the lzu Peninsula. Japan. J. Plankton Res.
8, 1039-1049.

TsUJI, T., OHKI, K. and FUJITA, Y. (1986).

Determination of photosynthetic pigment composition in an individual phytoplankton cell in seas andlakes using fluorescence microscopy; properties of the fluorescence emitted from picophytoplankton cells. Marine Biology 93. 343-349.
1987.

FALKOWSKI, P.G., and FUJITA, Y. (1987).

Effect of light state transitions on the apparent absorption cross section of photosystem II in Chiarella.
In "Progress in Photosynthesis" (Biggins. J. ed.). Vol.ll pp.737-740, Proceedings of the Vllth lhternational Congress on Photosynthesis.
Martinus Nijhoff Publishers, Dordrecht.

P., MIMURO, M., SUTER, F. and ZUBER, H. (1987).
Alloophycocyanin complexes of the phycobilisome from Mastigocladus
laminosus. Influence of the linker poly-peptide Lc 8 ·9 on the spectral properties of the phycobili-protein subunits. Bioi. Chem.
Hoppe-Seyler. 368, 353-367.

FUGLISTALLER,

32

FUJITA, V., OHKI, K. and MURAKAMI, A. {1987).

Chromatic regulation of photosystem composition in the cyanobacterial photosynthetic system :Kinetic relationship between changes of photosystem composition and cell proliferation. Plant Cell Physiol. 28, 227-234.

FUJITA, V., MURAKAMI, A. and OHKI, K. {1987).

Regulation of photosystem composition in the cyanobacterial photosynthetic system: The
regulation occurs in response to the redox steady state of the electron pool located between two photosystems. Plant Cell Physiol. 28,
283-292.

FUJITA, V. and MURAKAMI, A. {1987).

Regulation of electron trasport commposition in cyanobacterial photosynthetic system : Stoichiometry
among photosystem I and II complexes and cytchrome b61f complex. Plant Cell Physiol. 28, 1547-1553.

HATA·TANAKA, A., MATSUURA, K., ITOH, 5. and ANRAKU, V. {1987).

Electron flow and heme-interaction between cytochromes b-558, b-595 and din a terminal oxidase of Escherichia coli. Biochim. Biophys. Acta 893, 289-295.
Characterization of photosystem I chlorophyll-protein complexes reconstituted into phosphatidylcholine
liposomes. Plant Cell Physiol. 28, 599-609.

HOSHINA, 5. and ITOH, 5. {1987).

Characterization by EPR spectroscopy of cytochrome b-562 isolated from thecytochrome b-c 1 complex of Rhodopseudomonas spheroides R-26. In "Progress in Photosynthesis" (Biggins. J . ed.), Vol.ll pp.497-500,
Proceedings of the Vllth International Congress on Photosynthesis, Martinus Nijhoff Publishers. Dordrecht.

IBA, K., TAKAMIYA, K., NISHIMURA, M. and ITOH, 5. {1987).

Evidence for the interaction of the photosystem I secondary electron acceptorx X with chlorophyll a in spinach
photosystem I particles. Biochim . Biophys. Acta 893, 517-523.

IKEGAMI, I. and ITOH, 5. {1987).

ISOGAI, V., NISHIMURA, M. and ITOH, 5. {1987).

Charge distribution on the membrane surface of the donor side of photosystem II. Effect of thefree radical relaxing agent dysprosium on the power saturation of EPR signal lis in PS-11 particles. Plant Cell Physiol. 28, 1493-1499.

ITOH, 5., IWAKI, M. and IKEGAMI, I. {1987).

Extraction of vitamin k-1 from photosystem I particles by treatment with diethyl ether and its effects
on the A- EPR signal and photosystem I photochemistry. Biochim. Biophys. Acta 893, 508-516.

Spatial relationship between the intramembrane components (D+, z +) which give rise tosignal II and the membrane peripheral proteins working in photosystem II oxygen evolution studied by the effect of spin-relaxing reagent
dyprosium. In "Progress in Photosynthesis Research" (Biggins. J. ed.) Vol.l pp.483-486, Proceedings of the Vllth International Congress
on Photosynthesis. Martinus Nijhoff Publishers. Dordrecht.

ITOH, 5., lsOGAI, V., TANG, X.-5. and 5ATOH, K. {1987).

V. and ITOH, 5. {1987).
Formation of blue membrane of bacteriorhodopsin by addition of tetrakis(4-fluorophenyl) boron. a hydrophobic anion. Biochim. Biophys. Acta 904, 179-186.

KAMO, N., YosHIMOTO, M., KOBATAKE,

33

Excitation energy transfer in spinach chloroplasts . Analysis by the time-resolved
fluorescence spectrum at -196°C in the picosecond time range. FEBS Let. 213, 119-122.

MIMURO, M., TAMAI, N., YAMAZAKI, T. and YAMAZAKI, I. (1987).

Analysis of pigment system I chlorophyll a fluorescence at room
temperature by the steady state spectrum and the time resolved-spectrum in picosecond time range. In "Progress in Photosynthesis
Research" (Biggins, J. ed.). Vol.l 91-94, Proceedings of the Vllth International Congress on Photosynthesis, Martinus Nijhoff Publishers.
Dordrecht.

MIMURO, M., YAMAZAKI, I., TAMAI, N., YAMAZAKI, T. and FUJITA, Y. (1987).

Analysis of the excitation energy transfer in spinach chloroplasts atroom temperature. Identification of the component bands by the time-resolved fluorescence spectrum and by convolution of the decay
kinetics. In "Primary Processes in Photobiology" (Kobayashi, T. ed.). pp.23-32, Proceedings of the 12th Taniguchi Symposium,
Springer-Verlag, Berlin.

MIMURO, M., YAMAZAKI, T., TAMAI, N., YAMAZAKI, T. and FUJIT , Y. (1987).

Non-hemidiscoidal phycobilisome in cyanophytes. In "Progress in Photosynthesis Research". (Biggins. J. ed.).
Vol.ll 157-160, Proceedings of the VII International Congress on Photosynthesis, Martinus Nijhoff Publishers, Dordrecht.

OHKI, K. and FUJITA, Y. (1987).

OHKI, K., OKABE, Y., MURAKAMI, A. and FUITA, Y. (1987).

A comparative study of quantitative relationship between phycobiliproteins andphotosystem II in cyanobacteria and red algae. Plant Cell Physiol. 28, 1219-1226.

Purification and properties of an oxygen-evolving photosystem II reaction-center
complex from spinach. Biochim. Biophys. Acta 891, 129-137.

YAMADA, Y., TANG, X.-S., ITOH, S. and SATOH, K. (1987).

Photonic energy transport in phycobilin-chlorophyll system: A
comparative study with artificial multilayer films. In "Primary Processes in Photobiology" . (Kobayashi, T. ed.). pp.33-42, Proceedings ofthe 12th Taniguchi Symposium, Springer-Verlag, Berlin.

YAMAZAKI, I.,TAMAI, N., YAMAZAKI, T., MIMURO, M and FUJITA, Y. (1987).

DIVISION OF CELL FUSIONKHONO, K., UCHIDA, T., OKUBO, H., NAKANISHI, S., NAKANISHI, T., FUKUI, T., 0HTSUKA, E. IKEHARA, M. and OKADA, Y. (1986).

Amino acidsequence of mammalian elongation factor 2 deduced from the eDNA sequence : homology with GTP-binding proteins. Proc. Natl. Acad.
Sci. U.S.A. 83, 4978-4982.
Highly frequent single amino acid substitution in mammalian elongation factor 2 (EF-2) results in expression ofresistance to EF-2-ADP-ribosylating toxins . J. Bioi. Chem. 262, 12298-12305.

KHONO, K. and UCHIDA, T. (1987).

34

KHONO, K. and UCHIDA, T. (1987).
172. 54-64.

Mutant with diphtheria toxin receptor and acidification function but defective in entry of toxin. Exp. Cell. Res.

KANEDA, Y., UCHIDA, T., KIM, J., ISHIURA, M. and OKADA, Y. (1987).

The improved efficient method for introducing macromolecules into cells
using HVJ(Sendai virus)-liposomes with gangliosides. Exp. Cell Res . 173, 56-69.

DEPARTMENT OF DEVELOPMENTAL BIOLOGYDIVISION OF REPRODUCTIVE BIOLOGYKISHIMOTO, T. (1986).

Microinjection and cytoplasmic transfer in starfish oocytes. In "Echinoderm Gametes and Embryos". (Schroeder, T.E. ed .)Methods in Cell Biology. Vol.27, pp.379-394. Academic Press, New York.

KISHIMOTO, T. (1986).

Maturation-promoting factor in starfish oocytes : Preliminary characterization and action on oocyte-follicle cell junctions. In"Advances in Invertebrate Reproduction". (Porchet, M .. Andries, J.C. and Dhainaut. A. eds.) Vol.4, pp.257-264. Elsevier Sci. Pub. BV.
Amsterdam .

KISHIMOTO, T. and KONDO, H. (1986).
Res. 163, 44~52.

Extraction and preliminary characterization of maturation-promoting factor from starfish oocytes. Exp. Cell

KONDO, N., SHIRAI, H., BULET, P., KANATANI, H., ISOBE, M., IMAI, K. and GOTO, T. (1986).

Effective desalting techniq1.,1es for a hormonal peptide,

gonad-stimulating substance. of starfishes. Biomed. Res. 7. 89-95.
MIYAZAKI, S., HASHIMOTO, N., YOSHIMOTO, Y., KISHIMOTO, T., IGUSA, Y. and HIRAMOTO, Y. (1986).

Temporal and spatial dynamics of the periodic
increase in intracellular free calcium at fertilization of golden hamster eggs. Dev. Bioi. 118, 259-267 .
Testis. In "Vertebrate Endocrinology: Applications to Implications" . (Pang, P.K.T. and Schreibman. M.P. eds.) Vol.1,
pp.399-437. Academic Press, New York.

NAGAHAMA, Y. (1986).

NAGAHAMA, Y., GOETZ, F.W. and TAN, J.D. (1986).
Growth and Differ. 28, 555-561 .

Shift in steroidogenesis in the ovarian follicles of the goldfish (Carassius auratus). Develop.

Gonad-stimulating and maturation-inducing subtance. In "Echinoderm Gametes and Embryos" . (Schroeder. T.E. ed.) Methods
in Cell Biology Vol.27. pp.73-88. Academic Press. New York.

SHIRAI, H. (1986).

35

SHIRAI, H., BULET, P., KONDO, N., ISOBE, M., IMAI, K. and GOTO, T. (1986).

Endocrine control of oocyte maturation and spawning in starfish. In"Advances in Invertebrate Reproduction 4". (Porchet, M .. Andries, J.-C. and Dhainaut, A. eds.) pp.249-256. Elsevier Sci. Pub.,
Amsterdam.

SHIRAI, H., KONDO, N., BULET, P., KANATANI, H., ISOBE, M., IMAI, K. and GOTO, T. (1986).

Separation by preparative electrofocusing of several
components of gonad-stimulating substance of starfish. Biomed. Res. 7, 97-102.

TAN, D.J., ADACHI, S. and NAGAHAMA, Y. (1986).

The in vitro effects of cyclic nucleotides. cyanoketone. and cycloheximide on the production of
estradiol-17,8 by vitellogenic ovarian follicles of goldfish (Carassius auratus). Gen. Comp. Endocrinol. 63, 110-116.

UEDA, H., KAGAWA, H., NAGAHAMA, Y. and FUJIMOTO, S. (1986).

Immunocytochemistry of teleost gonadotrophs and thyrotrophs. In "Pars Distalisof the Pituitary Gland-Structure. Function and Regulation". (Yoshimura. F. and Gorbman, A. eds.) pp.81-83. Elsevier Sci. Bub. BV.
Amsterdam.

YouNG, G., ADACHI, S. and NAGAHAMA, Y. (1986).

Role of ovarian thecal and granulosa layers in gonadotropin-induced synthesis of a salmonid
maturation-inducing substance (17a,20,8-dihydroxy-4-pregnen-3-one). Dev. Bioi. 118, 1-8.

ADEYEMO, 0., SHIRAI, H. and KOIDE, S.S. (1987).
Gamete Res. 16, 251-258.

Cyclic nucleotide content and protein phosphorylation during maturation of Spisula oocytes.

and ONITAKE, K. (1987).
Induction and inhibition of in vitro oocyte maturation andproduction of steroids in fish follicles by forskolin . J. Exp. Zool. 241, 101-111 .

IWAMATSU, T., TAKAHASHI, S.Y., SAKAl, N., NAGAHAMA, Y.

H. and NAGAHAMA, Y. (1987).
Gonadotropin receptors in the postovulatory ovary of amago salmon (Oncorhynchus
rhodurus). Gen. Comp. Endocrinol. 66, 210-217.

KANAMORI, A., KAGAWA,

KAWAUCHI, H., SUZUKI, K., ITOH, H., SWANSON, P. and NAGAHAMA, Y. (1987).

Duality of salmon pituitary gonadotropins. In "Proc. of 1st Congressof the Asia and Oceania Society for Comparative Endocrinology". (Ohnishi, E., Nagahama. Y. and lshizaki. H. eds.) pp.15-18. Nagoya
University Coorporation, Nagoya.

MITA, M., UETA, N. and NAGAHAMA, Y. (1987).

Regulatory functions of cyclic adenosine 3',5'-monophosphate in 1-methyladenine production by
starfish follicle cells. Biochem. Biophys. Res. Commun. 147, 8-12.

MITA, M., UETA, N. and NAGAHAMA, Y. (1987).

In vitro induction of starfish oocyte maturation by cysteine alkylesters. Develop. Growth andDiffer. 29, 607-616.
NAGAHAMA, Y. (1987).

36

17a,20,8-Dihydroxy-4-pregnen-3-one: A teleost maturation-inducing hormone. Develop. Growth and Differ. 29, 1-12.

Endocrine control of oocyte maturation. In "HormorJes and Reproduction il'l Fishes, Amphibians and Rej:>tiles". (Norris,
D.O. and Jones, R.E. eds.) pp.171-202. Plenum Press, New York.

NAGAiifAMA, Y. (1987).

NAGAHAMA, Y. (1987).

Gonadotropin action on gametogenesis and steroidogenesis in teleost gonads. Zool. Sci. 4, 209-222.

NAKAMURA, M., NAGAHAMA, V., IWAHASHI, M. and KOJIMA, M. (1987).

Ovarian structure and plasma steroid hormones of triploid female rainbow

trout. Nippon Suisan Gakkaishi 53, 1105.
Development of the steroidogenic capacity of medaka (Oryzias latipes)
ovarian follicles during vitellogenesis and oocyte maturation. Gen. Comp. Endocrinol. 66, 333-342.

SAKAl, N., IWAMATSU, T., YAMAUCHI, K. and NAGAHAMA, Y. (1987).

Chemical control of asexual and sexual reproduction in echinoderms. In "Endocrinology of Selected
Invertebrate Types. Invertebrate Endocrinology, Vol.2, V. Echinoderms" . (Laufer, H. and Downer, G.H . eds.) pp.453-476. Alan R. Liss,
Inc., New York.

SHIRAI, H. and WALKER, C,W. (1987).

TACHIBANA, K., VANAGISHIMA, N. and KISHIMOTO, T. (1987).
cerevisiae. J. Cell Sci. 88, 273- 281 .

Preliminary characterization of maturation-promoting factor from yeast Saccharomyces

TORAVA, T., NAGAHAMA, V., KANATANI, H. and KOIDE, S.S. (1987).

A factor potentiating serotonin in the induction of germinal vesicle breakdown

in the surf clam oocytes. Experientia 43, 885-886.

DIVISION OF CELL DIFFERENTIATIONKUSUDA, J., TAZIMA, V., ONIMARU, K., NINAKI, 0., and SUZUKI, V. (1986).

The sequence around the 5' end of fibroin gene from the wildsilkworm, Bombyx mandarina, and comparison with that of the domesticated species, B. mori. Mol. & Gen . Genetics 203, 359- 364.

TSUDA, M., HIROSE, S., and SuzuKI, Y. (1986).

Participation of the upstream region of the fibroin gene in the formation of transcription complex
in vitro. Mol. & Cell. Bioi. 6, 3928-3933.

SUZUKI, V., TSUDA, M., TAKIVA, S., HIROSE, S., SUZUKI, E., KAMEDA, M., and NINAKI, 0. (1986).

Tissue-specific transcription enhancement of the
fibroin gene characterized by cell-free systems. Proc. Natl. Acad . Sci . USA 83, 9522-9526.

SUZUKI, V., TSUDA, M., HIROSE, S., and TAKIVA, S. (1986).

Transcription signals and factors of the silk gemes. In "Genetics Systems, Advances inBiophysics". Vol.21, (Ozeki, H., Osawa, S., Uchida, H., Shimura, Y. and lshihama, A. eds .). pp.205-215, Japan Scientific Societies Pressand Springer-Verlag, Tokyo and Berlin.

37

HAMADA, Y., YAMASHITA, 0., and SUZUKI, Y. (1987).

Haemolymph control of sericin gene expression studied by organ culture. Cell Differentiation

20, 65-76.

The fibroin genes of Antheraea yamamai and Bombyx mori that are different in their core coding
regions reveal a striking sequence similarity at the 5' ends and 5' flanking regions. Mol. & Gen. Genetics 207, 189-195.

TAMURA, T., INOUE, H., and SuzuKI, Y. (1987).

Changes of DNasel sensitivity of the fibroin and sericin genes during the silkworm development. Develop.
Growth & Differ. 29, 363-372.

KOGA·BAN, Y. and SuZUKI, Y. (1987).

SAKIMURA, K., KUSHIYA, E., TAKAHASHI, Y., and SUZUKI, Y. (1987).

The structure and expression of neuron-specific enolase gene. Gene 60,

103-113.
SuzuKI, Y., TAKIYA, S., HARA, W., OBARA, T., SuzuKI, T., and HUI, C.-C. (1987).

Developmental regulation of the tissue- specific genes and thehomeotic genes in Bombyx mori. In "Gunma Symposia on Endocrinology" . Vol.24, (lwai, K. ed.). pp.13-26, Center for Acad . Publ.
Japan, Tokyo, and VNU Science Press BV, Utrecht.
T3 action and chromatin structure. In "Gunma Symposia on Endocrinology" . Vol.24, (lwai. K.
ed.). pp.155-168, Center for Acad . Publ. Japan, Tokyo, and VNU Science Press BV.

KITAGAWA, M., OHBA, Y., and SUZUKI, Y. (1987).

DIVISION OF MORPHOGENESISEGUCHI, G. (1986).

Instability in cell commitment of vertebrate pigmented epithelial cells and their transdifferentiation into lens cel ls. In " Current
Topics in Developmental Biology 20 : Instability in Cell Commitment and Transdifferentiation". (Okada, T.S. and Moscona. A.A. eds.),
pp.21-37, Academic Press. Japan Inc. Tokyo.

EGUCHI,

G. (1986).

Environmental Regulation of the differentiated states of pigmented epithelial cells in vitro . In "Cellular Endocrinology :Hormonal Control of Embryonic and Cellular Differentiation" . (Hayashi, J. and Serrero, G. eds.). pp.307-318, Alan R. Liss. Inc.

ARITA, T., OKAMURA, R., KODAMA, R., and EGUCHI, G. (1986).

In vitro system of corneal endothelium. Japanese Journal of Ophthalmology, 30.

233--237.
AKIYAMA, T., MATSUMOTO, J., ISHIKAWA, T., and EGUCHI, G. (1986).

Production of crystallins and lens-like structures in differentiation-induced
neoplastic pigment cells (goldfish erythrophoroma cells) in vitro. Differentiation 33, pp.34-44.

Transformation of a polygonal cellular pattern during sexual maturation of the avian oviductepithelium : computer simulation. J. Embryo!. exp. Morp 98, 1-19.

HONDA, H., YAMANAKA, H., and EGUCHI, G. (1986).

38

Enhancement of expression of lens phenotype in cultures of pigmented epithelial cells by hyaluronidase in thepresence of phenylthiourea. Cell Differentiation. 18, 173- 182.

ITOH, V., and EGUCHI, G. (1986).

In vitro analysis of cellular metaplasia from pigmented epithelial cells to lens phenotypes: A unique model
system for studying cellular and molecular mechanisms of "Transdiffernetiation" . Developmental Biology. 115, 353-362.

ITOH, Y. and EGUCHI, G. (1986).

I<ADOKAWA, V., DAN-SHOHKAWA, M., and EGUCHI, G. (1986).
Studies on the mechanism of blastula formation in starfish embryos denuded offertilization membrane. Cell Differentiation. 19, 79-88.

Immunological analysis of 6-crystallin expressed in various tissues of the chick in vivo and in vitro. In"Coordinated Regulation of Gene Expression" (Clayton. R.M . and Truman. D.E.S. eds.). pp.293-297.

KODAMA, R., and EGUCHI, G. (1986).

Multipotentiality in differentiation of the pineal as revealed by cell culture. In "Current Topics in Developmental Biology
20 : Instability in Cell Commitment and Transdifferentiation" (Okada, T.S. and Moscona. A.A. eds.). Academic Press. Japan Inc. Tokyo,
pp.89-97.

WATANABE, K. (1986).

ARAKI, M., IIDA, V., TKETANI, S., WATANABE, K. 0HTA, T. and SAITO, T. (1987).

Characterization of photoreceptor cell differentiation in the rat

retinal cell culture. Developmental Biology 124, 239-247 .
ARITA, T., OKAMURA, R., KODAMA, R., TAKEUCHI, T., I<ADOYA, V., and EGUCHI, G. (1987).

Density dependent growth of corneal endothelial cells

cultured in vitro. Cell Differentiation 22, 61-70.
I<ADOKAWA, V., I<ATO, V., and EGUCHI, G. (1987).

Cell lineage analysis of the primitive and visceral endoderm of mouse embryos cultured invitro. Cell Differentiation, 21 , 69- 76.
Immunological relationships
among embryonic and adult chicken pepsinogens : A study with monoclonal and polyclonal antibodies. Develop. Growth and Differ., 29.
85-91.

VASUGI, S., HAYASHI, K., TAKIGUCHI, K., MIZUNO, T., MOCHII, M. KODAMA, R., AGATA, K., and EGUCHI, G. (1987).

39

DEPARTMENT. OF BIOLOGICAL REGULATION
DI~ISION
NAKA, K.-1., ITOH, M.-A.

and CHAPPELL, R.L. (1987).

SAKURANAGA, M., ANDO, V.-I. and NAKA, K.-1. (1987).

OF SENSORY PROCESSING
Dynamics of turtle cones. J. Gen. Physiol. 89, 321-337.
Dynamics of ganglion,cell response in the catfish and frog retinas. J. Gen. Physiol. 90,

229-259.
SAKAl, H.M. and NAKA, K.-1. (1987).
SAKAl, H.M.

and NAKA, K.-1. (1987).

Signal transmission in the catfish retina. IV Transmission to ganglion cells. J. Neurophysiol. 58, 1307-1328.
Signal transmssion in the catifish retina . V Sensitivity and circuit. J. Neurophysiol. 58. 1329- 1350.

SAKAl, H.M. and NAKA, K.-1. (1987).

Neuron network in catfish retina : 1968-1987. In "Progress in Retinal Research" . Vol.7, (Osborne, N.N. andChader. G.J . eds.). pp.149-208.

DIVISION OF CELLULAR REGULATIONBISHOP,

D.G., KENRICK, J.R., KONDO, T. and MURATA, N. (1986).

Thermal properties of membrane lipids from two cyanobacteria, Anacystis
nidulans and Synechococcus sp. Plant Cell Physiol. 27. 1593-1598.

KUWABARA, T., MURATA, T., MIYAO, M. and MURATA, N. (1986).

Partial degradation of the 18-kDa protein of the photosynthetic oxygen-evolving
complex : a study of a binding site. Biochim. Biophys. Acta . 850, 146-155.

MIYAO, M.

and MURATA, N. (1986).
Light-dependent inactivation of photosynthetic oxygen evolution during NaCI treatment of photosystem IIparticles: The role of the 24-kDa protein. Photosyn. Res . 10, 489-496.

MURATA, N. and SATOH, K. (1986).

Absorption and fluorescence emission by intact cells. chlorophyll-protein complexs . In "Light Emission by
Plants and Bacteria" (Govindjee, Amesz, J. and Fork, D.C. eds.). pp.137-159, Academic Press, Orlando, FL.

MURATA, N., ARAKI, S., FUJITA, V., SUZUKI, K., KUWABARA, T . and MATHIS, P. (1986).

Stoichiometric determination of pheophytin in photosystem

II of oxygenic photosynthesis. Photosyn. Res. 9, 63-70.

and MURATA, N. (1986).
spinach photosystem II particles. FEBS Lett. 197. 63-66.

0H-0KA, H., TANAKA, S., WADA, K., KUWABARA, T.

40

Complete amino acid sequence of 33 kDa protein isolated from

Glucolipid synthesis activities in cytoplasmic and thylakoid membranes from the cyanobacterium Anacystis
nidulans. Plant Cell Physiol. 27, 485-490.

OMATA, T. and MURATA, N. (1986).

Lipid-linked desaturation of palmitic acid in monogalactosyl diacylglycerol in the blue-green alga
(cyanobacterium) Anabaena variabilis studied in vivo. Plant Cell Physiol. 27, 819-835.

SATO, N., SEVAMA, Y. and MURATA, N. (1986).

ARAKI, S., SAKURAI, T., KAWAGUCHI, A., I<AYAMA, M. and MURATA, N. (1987).

Positional distribution of fatty acids in glycerolipids from the redalga, Porphyra yezoensis . Plant Cell Physiol. 28, 761-766.
Properties of the plastidial acyl-(acyl-carrier protein): glycerol-3-phosphate acyltransferase fromthe chilling-sensitive plant squash (Cucurbita moschata). Plant Cell Physiol. 28, 1195-1201.

FRENTZEN, M., NISHIDA, I. and MURATA, N. (1987).

Photoinhibition of the oxygen-evolving complex of photosystem II. In "Topics in Photosynthesis", Vol.9 :Photoinhibition (Kyle, D., Osmond, B. and Arntzen, C.J. eds.), pp.289-307, Elsevier Science Publishers. Amsterdam.

MIYAO, M. and MURATA, N. (1987).

MIYAO, M., MURATA, N., lAVOREL, J., MAISON-PETERI, 8., BOUSSAC, A. and ETIENNE, A.·l. (1987).

Effect of the 33-kDa protein on the S-statetransitions in photosynthetic oxygen evolution . Biochim, Biophys. Acta. 890, 151-159.
Lipids of blue-green algae (cyanobacteria). In "The Biochemistry of Plants", Vol.9, Lipids: Structure andFunction (Stumpf, P.K. ed.), pp.315-347, Academic Press, Orlando, Florida.

MURATA, N. and NISHIDA, I. (1987).

Purification of isomeric forms of acyl-(acyl-carrier protein): glycerol-3-phosphate
acyltransferase from greening squash cotyledons. Plant Cell Physiol. 28, 1071-1079.

NISHIDA, 1., FRENTZEN, M., ISHIZAKI, 0. and MURATA, N. (1987).

NAKASHIMA, H. (1986).

Phase shifting by the calmodulin antagonists of circadian conidiation rhythm in Neurospora crassa. J. Bioi. Rhythms. 2,

163-169.
Comparison of phase shifting by temperature of wild type Neurospora crassa and the clock mutant. frq-7. J. lnterdiscipl.
Cycle Res. 18. 1-8.

NAKASHIMA, H. (1987).

CORNELIUS, G. and NAKASHIMA, H. (1987).

Neurospora crassa vacuoles play a decisive role in calcium homeostasis. J. Gen. Microbial. 133,2341-2347.
Removal by yeast extract of inhibition by diethylstilbestrol of growth and phase-shifting in Neurospora crassa. Plant Cell
Physiol. 28, 1477-1483.

NAKASHIMA, H. (1987).

41

DIVISION OF BIOLOGICAL REGULATION
Effects of gabaculine on phytochrome synthesis during imbibition in embryonic axes of Pisum sativum L.
Plant Cell Physiol. 27, 1507-1512.

KoNOMI, K. and FURUYA, M. (1986).

LUMSDEN, P.J. and FURUYA, M. (1986).

Evidence for two actions of light in the photoperiodic induction of flowering in Pharbitis nil. Plant Cell

Physiol. 27, 1541-1551.
LUMSDEN, P.J., VINCE-PRUE, 0. and FURUYA, M. (1986).
pulses. Physiol. Plant. 67, 604-607.

Phase-shifting of the photoperiodic flowering response rhythm in Pharbitis nil by red-light

Temperature dependence of absorption spectra of 114k dalton pea
phytochrome and relative quantum yield of its phototransformation. Photobiochem. Photobiophys. 12, 243-251.

SASAKI, N., OJI, Y., YOSHIZAWA, T., YAMAMOTO, K.T. and FURUYA, M. (1986).

SATO, N., TOMIZAWA, K., KOMEDA, Y. and FURUYA, M. (1986).

Partial enrichment of pea phytochrome mRNA by gel electrophoresis and transfer

to poly(U)-paper. Plant Cell Physiol. 27, 183-186.
Effect of pH on absorption spectra of ·pea 114 and 121 kilodalton
phytochromes during and after red-light irradiation. Plant Cell Physiol. 27, 765-773.

TOKUTOMI, S., INOUE, Y., SATO, N., YAMAMOTO, K.T. and FURUYA, M. (1986).

TOMIZAWA, K., KOMEDA, Y., SATO, N., NAGATANI, A., IINO, T. and FURUYA, M. (1986).

Isolation of eDNA for pea phytochrome using an

expression vector. Plant Cell Physiol. 27, 1101-1108.
FURUYA, M. (1987).

The history of phytochrome. In "Phytochrome and Photoregulation in Plants". (Furuya, M. ed.). pp.3-8, Academic Press.

Tokyo.
Changes in the content of phytochrome I and II apoproteins in embryonic axes of pea seeds
during imbibition. Plant Cell Physiol. 28, 1443-1451.

KONOMI, K., ABE, H. and FURUYA, M. (1987).

Action spectra confirm two separate actions of phytochrome in the induction of flowering in
Lemna paucicostata 441 . Plant Cell Physiol. 28, 1237-1242.

LUMSDEN, P.J., SAJI, H. and FURUYA, M. (1987).

Properties of membrane-associated phytochrome in peas. In "Phytochrome and Photoregulation in Plants". (Furuya, M. ed.),pp.209-214, Academic Press, Tokyo.

MANABE, K. (1987).

Application of monoclonal antibodies to phytochrome studies. In "Phytochrome
and Photoregulation in Plants". (Furuya, M . ed.), pp.95-114, Academic Press, Tokyo.

NAGATANI, A., LUMSDEN, P.J., KONOMI, K. and ABE, H. (1987).

42

SATO, R., NAGANO, E., 0SHIO, H., I<AMOSHITA, K. and FURUYA, M. (1987).

Wavelength effect on the action of a N-phenylimide S-23142 and adiphenylether acifluorfen-ethyl in cotyledons of cucumber (Cucumis sativus L.) seedlings. Plant Physiol. 85, 1146-1150.

TOKUTOMI, S., KATAOKA, M. and TOKUNAGA, F. (1987).

Small-angle X-ray scattering, a useful tool for studying the structure of phytochrome. In"Phytochrome and Photoregulation in Plants" . (Furuya, M . ed.), pp.167-177, Academic Press, Tokyo.

Structure-function relationship of pea phytochrome as deduced from amino-terminal sequence analysis of phytochrome
chromopeptides. In "Phytochrome and Photoregulation in Plants" . (Furuya, M. ed.). pp.63-81, Academic Press, Tokyo.

YAMAMOTO, K.T. (1987).

DIVISION OF BEHAVIOR AND NEUROBIOLOGYKOHSAKA, S., YOSHIDA, K., INOUE, Y., SHINOZAKI, T., TAKAYAMA, H., INOUE, H., MIKOSHIBA, K., TAKAMATSU, K., OTANI, M., TOVA, S., TSUKADA, V.
(1986).
Transplantation of bulkseparated oligodendrcytes into the brains of shiverer mutant mice : Immunohistochemical and electron

microscopic studies on the myelination. Brain Research 372, 137-142.
INOUE, Y., MIKOSHIBA, K., YOKOYAMA, M., INOUE, K. TERASHIMA, T., NOMURA, T. and TSUKUBA, Y. (1986).

Alteration of the primary pattern ofcentral myelin in a chimaeric environment-study of shiverer-wild-type chimaeras. Developmental Brain Research 26, 239-247.

TAKAMIYA, V., KOHSAKA, S., TOYA, S., OTANI, M., MIKOSHIBA, K., TSUKADA, Y. (1986).

Possible association of platelet-derived growth factor(PDGF) with the appearance of reactive astrocytes following abarain injury in situ. Brain Research, 383, 305-309.

Studies on
the development and growth of the mammalian nervous system by aggregation chimeras: Analysis of coticohissto-genesis in the
cerebrum by reeler mutant mice. In "Progress in Developmental Biology", Part B, (Harold, C. Slavkin ed.), pp.137- 140, Alan JR. Liss.
Inc. New York.

MIKOSHIBA, K., YOKOYAMA, M., TERASHIMA, H., NISHIMURA, Y., INOUE, Y., OKANO, H., TAKAMATSU, K., NOMURA, T ., TSUKADA, Y. (1986).

Ataxic mutant animals: morphological and neurochemical studies on the cerebellar mutants. In "TRH &Spinocerebellar Degeneration" . (Sobue, I. ed.). pp.125- 134.

MIKOSHIBA, K., OKANO, H. (1986).

KIMURA, M ., KATSUKI, M., INOKO, H., ANDO, A ., SATO, T., HIROSE, T . INAYAMA, S., TAKASHIMA, H., TAKAMATSU, K., MIKOSHIBA, K., TSUKADA, V.,
YOKOYAMA, M., WATANABE, I. (1986).
Sturucture and expression of the myelin basic protein gene in mouse. In "Molecular Genetics

in Developmental Neurobiology" . (Tsukada, Y. ed.), pp.125-136, Japan Scientific Societies Press, Tokyo, VNU Science Press BV. Utrecht.
The Netherlands.

43

OKANO, H., MIURA, M., MIKOSHIBA, K., INOUE, V., FUJISHIRO, M.

and TSUKADA, V. (1986).
Inefficient transcription of the myelin basic protein(MBP) gene is a possible cause of hypomyelination in myelin dificient (mid) mutant mice. In "Molecular Genetics in Developmental
Neurobiology" (Tsukada, Y. ed.). pp.187-199. Japan Scientific Societies Press, Tokyo, VNU Science. Press BV, Utrecht, The Netherlands.

OKANO, H., MIURA, M., MORIGUCHI, A., IKENAKA, K., TSUKADA, V. and MIKOSHIBA, K. (1987).

Inefficient transcription of the myelin basic protein
gene possibly causes hypomyelination in myelin-deficient mutant mice. J. Neurochemistry 48, 470-477.

TERASHIMA, T., INOUE, K., INOUE, V., MIKOSHIBA, K. (1987).

Thalamic connectivity of the primary motor cortex of normal and reeler mutant mice.

J. Comparative Neural. 257, 405-421.

V., TAKAMATSU, K., LACHAPELLE, F., BAUMANN, N. and TSUKADA, V. (1987).
Immunohistochemical, biochemical and electronmicroscopic analysis of myelin formation in the central nervous system of myelin deficient mutant(mid) mice. Dev. Brain Research 35, 111-121.

MIKOSHIBA, K., OKANO, H., FUJISHIRO, M., INOUE,MORIGUCHI, A., IKENAKA, K., FURUICHI, T., OKANO, H., IWASAKI, V., MIKOSHIBA, K. (1987).

The fifth exon of the myelin proteolipid protein (PLP)

gene is not utilized in the brain of jimpy mutant mice. Gene 55, 333-337.

LARGE SPECTROGRAPH LABORATORY
and FURUYA, M. (1986).
Inhibition of motility in the cyanobacterium, Phormidium uncinatum, by solar andmonochromatic UV irradiation. Pland Cell Physiol. 27, 887-894.

HADER, D.-P., WATANABE, M.

HASUNUMA, K., FUNADERA, K., SHINOHARA, V., FURUKAWA, K.

and WATANABE, M. (1987).

Circadian oscillation and light-induced changes in theconcentration of cyclic nucleotides in Neurospora. Curr. Genet. 12, 127-133.

and WATANABE, M. (1987).
Partial characterization and light-induced regulation of
GTPj-binding proteins in Lemna paucicostata. Photochem. Photobiol. 46, 531-535.

HASUNUMA, K., FURUKAWA, K., FUNADERA, K., KUBOTA, M.

TSUJIMOTO, K., OHMURA, K., OHASHI, M., HARA, R., HARA, T., OZAKI, K. and WATANABE, M. (1986).

Highly stereoselective photoisomerization of
all-trans naphthyl retinal analog to the 11-cis isomer by protein mediation. Chem. Lett. 1986. 2005-2008.

YANG, K.-C., PRUSTI, P.K., WALKER, E.B., SONG, P.-S., WATANABE, M.

and FURUYA, M. (1986).

sensitized by endogenous pigment stentorin. Photochem. Photobiol. 43, 305-310.

44

Photodynamic action in Stentor coeruleus

TISSUE AND CELL CULTURE LABORATORY
HAMADA, Y., YAMASHITA, 0. and SUZUKI, V. (1987).

Haemolymph control of sericin gene expression studied by organ transplantation. Cell Differ.

20, 65- 76.

An effecient isolation method for polyadenylated messenger ribonucleic acid from
Neurospora mycelia. Fung. Genet. Newslett. 34, 30- 31 .

FURUKAWA, K., HASUNUMA, K. and HAMADA, V. (1987).

SOEBANDRIO, A., AzUMA, T., HAMADA, V., SAKATO, N. and FUJIO, H. (1987).

Specificity change of antibody to (4-hydroxy-3-nitrophenyl) acetyl

haptens by somatic hypermutation. J. Biochem . 1337-1343.
Complete primary structure of vertebratesmooth muscle myosin heavy chain deduced from its complementary DNA sequence. implications on topography and function of
myosin . J. Mol. Bioi. 198, 143-157.

VANAGISAWA, M., HAMADA, V., KATSURAGAWA, V ., IWAMURA, M., MIKAWA, T. and MASAKI, V. (1987).

RADIOISOTOPE FACILITIESBiochemical characterization of mutants in cyclic nucleotide metabolism
showing rhythmic con idiation in Neurospora. In "Adv. Chronobiology" . Part A. (Pauly, J.E. and Scheving. L.E . eds.). pp.123-130. Alan R.
Liss. Inc.. New York.

HASUNUMA, K., SHINOHARA, V., FUNADERA, K. and FURUKAWA, K. (1987).

HASUNUMA, K. and FUNADERA, K. (1987).

GTP-binding protein(s) in green plant, Lemna paucicostata. Biochem . Biophys. Res. Commun. 143.

908-912.
HASUNUMA, K., FUNADERA, K., SHINOHARA, V., FURUKAWA, K. and WATANABE, M. (1987).

Circadian oscillation and light-induced changes in theconcentration of cyclic nucleotides in Neurospora . Curr. Genet. 12. 127-133.
HASUNUMA, K. and SHINOHARA, V. (1987).

Assay method and localization of GTP-binding proteins in Neurospora crassa . Fungal Genet. Newsl.

34. 33-35.

An efficient isolation method for polyadenylated messener ribonucleic acid from
Neurospora mycelia. Fungal Genet. Newsl. 34. 30-31.

FURUKAWA, K., HASUNUMA, K. and HAMADA, V. (1987).

Partial characterization and light induced regulation of
GTP-binding proteins in Lemna paucicostata. Photochem. Photobiol. 46, 531 - 536.

HASUNUMA, K., FURUKAWA, K., FUNADERA, K., KUBOTA, M. and WATANABE, M. (1987).

45

FURUKAWA, K., HASUNUMA, K. and SHINOHARA, Y. (1987).

Characterization of Pi repressible enzymes secreted in culture media by Neurospora

crassa wild type cells and null type mutants. J. Bacterial. 169, 4790-4795.
HASUNUMA, K., MIYAMOTO-SHINOHARA, Y. and FURUKAWA, K. (1987).

Partial characterization of GTP-binding proteins in Neurospora. Biochem.

Biophys. Res. Commun. 146. 1178-1183.
HASUNUMA, K., FURUKAWA, K., TOMITA, K., MUKAI, C. and NAKAMURA, T. (1987).

GTP-binding proteins in etiolated epicotyls of Pisum sativum{Alaska) seedlings. Biochem. Biophys. Res. Commun. 148, 133-139.
HASUNUMA, K. (1987).

Circadian oscillation of the concentration of cyclic 3',5'-AMP and -GMP and the mechanism of light acceptance in

Neurospora and Lemna. In "Symposium on Radioisotope and Radiation Technique in Agriculture". Cheju Applied Radioisotope ResearchInstitute.. Cheju, Korea. pp.25-61.

:& EB tet:&~l' (1987) .

m tet fr (1987) .

'J 7f-'.J--4)ti. *1C~1t~~~)ti~~ 1, il1z;;.::r~~)tim, pp.l35-142, B*~1t~~m. **it~~ .A..
~~O)~JU~:t!'H!i. ifi~~~1t~~~)ti 1, ~~;fit:NO)J&~At\.'. pp.141-162, 9=1!i!r,fll, :& EB tet fr, IMJ EB

fft ii~ (1987) .

292-298.

46~'IE (1987).

mT*, ~m. ~im, H~(*i), Jz.~.

~~• *= (1987).

'*' *1

~. ~

~

•

*· •
~

1E it, Jj\ ., ~ !ij (1987).

*iff~~~. 53~, 1105.

13 3t:~~=f (1987).

rM~t.dBHi~J. ~11::~. 59~,

13 3t:~~=f (1987).

ru&:liJ#.JO)!*I?Hlt:*J. l*l?t~l!:~'&O)J7 1--7-A (B*.l:t.XI*J?t)~~~~~). pp.186-191, ~Wtt±.

13 3t:~~

T- (1987).

r~.1~Hitlb#.IO)*Jir1:/(~M)J. ~#.!~1\/1''·7' ·:~7 (::t:B3~xeB, j~*~W. ll.JDJa:$, ~ll.J*.

160- 162.

*Hi1t=, :Om!JJJ~.

:O~Cffil!ij, ~*~~. liJH.t'iJJJ II), pp. 244- 245, ~~·Ji5.

ii 5B 1l1J ;il, ti /Rt~!B (1987).

J7:fJI~/:fJ~·O)tatBft 1).A' .b.

(1)

il{i, ~41~1~, 81-88.

mt~!B (1987).

J7:fJif/:fJ~·O)tatB•Ii 1J.A' .b.

(2)i11i, ~41~2~. 48-54.

ii 5B 1l1liiii],

347

STAFF MEMBERS (July 1988)
DIRECTOR GENERALTOKINDO S. OKADA, B. Sci. & D. Sci. (zoology, Kyoto Univ.).
Research Associate, Department of Zoology, Kyoto Univ. (1954-59).
Macaulley Fellow, Univ. Edinburgh (1957- 59), Lecturer, Department
of Zoology, Kyoto Univ. (1960- 61 ), and Associate Professor,(1962-66) . Carnegie Fellow, Department of Embryology, Carnegie
Institution of Washington (1964-65). Professor. Department ofBiophysics, Kyoto Univ. (1967-84), Lillie Fellow, Woods Hole Marine
Biological Laboratory (1974). Director General, NIBB (1984- ).
Professor Emeritus, Kyoto Univ. (1985- ).

PROFESSOR EMERITUS
NOBURO KAMIYA, B. Sci. (botany, Tokyo Imperial Univ.). D. Sci .
(botany, Univ. Tokyo). Dr. rer. nat. h. c. (Freir Univ. Berlin) Lecturer,
Univ. Tokyo (1943-49). Professor. Osaka Univ. (1949-77). Visiting
Professor. Princeton Univ. (1962-63). Visiting Professor. State Univ.
of NY at Albany (1969-70). Professor. NIBB (1977-81). Visiting
Professor. Dartmouth College (1981). Guest Professor. Univ. Bonn(1985). Professor Emeritus. NIBB. Member Japan Academy.
MASUTARO KUWABARA, B. Sci. & D. Sci . (Zoology, Hokkaido
Imperial Univ.•). Research Associate. Hokkaido Imperial Univ. (193343, Lecturer. Associate Professor. (1944-49). Professor, Kyushu
U'liv.. (1949-73). Dean, School of Science (1964-66). Professor,

48

Sophia Univ. (1973-77). Director-General (1977-83). President. ONRI
(1981-83) . Professor Emeritus, NIBB. Member NIBB Councile(1983- ).

YUKITO OOTA, B. Sci. (botany, Tokyo Imperial Univ.) & D. Sci .
(biology, Nagoya Univ.). Assistant Professor (1950- 61). Professor(1961-79) & Professor Emeritus, Nagoya Univ. Professor (1979- 83)& Professor Emeritus. NIBB.

PROFESSORSYASUHIRO ANRAKU, Division of Cell Proliferation . B. Sci., M . Sci .
& Ph.D. (pharmaceutical sciences, Univ. Tokyo) . Visiting Fellow, NIH(1965-67). Research Fellow, Stanford Univ. (1967-68), Research
Associate (1968). Faculty of Pharmaceutical Sciences, Univ. Tokyo,
Associate Professor (1970-76). Professor. Department of Biology,
Univ. Tokyo (1976- ), Adjunct Professor, NIBB (1987- ).
GORO EGUCHI, Division of Morphogenesis. B. Sci. & D. Sci.
(biology, Nagoya Univ.). Research Associate. Department of Biology,
Nagoya Univ. (1959-68). Associate Professor, Department ofBiophysics, Kyoto Univ. (1968-76), Professor, Institute for MoleGular
Biology, Nagoya Univ. (1976-84). Professor, NIBB (1983- ).
YOSHIHIKO FUJITA, Division of Bioenergetics. B.L.A. (liberal arts.
Univ. Tokyo). M. Sci. (biology, Univ. Tokyo) & D. Sci. (biochemistry,
Univ. Tokyo~. Research Associate, lnst. Appl. Microbial., Univ. Tokyo(1957- 67). Postdoctral Fellow, Univ. Texas (1962-64), Research
Associate, Univ. Texas (1965), Associate Professor, Ocean Res .
lnst. Univ. Tokyo (1967-77). Professor (1977-82). Professor, NIBB
(1982- ). Acting Director, NIBB (Feb.-Sept., 1984).
HIDEMASA IMASEKI, Division of Biological Regulation . B. Sci., M .
Sci. & D. Sci. (botany, Univ. Tokyo). Research Associate. Nagoya
Univ. (1959-69), Visiting Fellow, UCLA (1963-65). Associate Professor. Nagoya Univ. (1969-79). Visiting Professor, Fairchild Tropical
Garden (1972). Professor, Nagoya Univ. (1979- ). Adjunct Professor, NIBB (1987- ).
KATSUHIKO MIKOSHIBA. Division of Behavior and Neurobiology.
M.D. (physiology, Keio Univ. School of Medicine). Instructor, Keio
Univ. Med. School (1973-74). Assistant Professor (1974-82).
Associate Professor (1982-85). Postgraduate Fellow, Pasteur Institute (1976-77). Professor Institute for Protein Research. Osaka
Univ. (1985- ). Adjunct Professor, NIBB (1986- ).
NORIO MURATA, Division of Cellular Regulation . B. Sci., M . Sci .
& D. Sci. (biochemistry, Univ. Tokyo). Research Associate, Department of Biophysics and Biochemistry, Univ. Tokyo (1966-78),
Carnegie Fellow. Department of Plant Biology, Carnegie Institutionof Washington (1969-70). Associate Professor. Department of
Biology, Univ. Tokyo (1978-85). Professor, NIBB (1985- ).
YOSHITAKA NAGAHAMA. Division of Reproductive Biology . . B.
Sci., M. Sci. & D. Fish. (biology, Hokkaido Univ.). Postdoctoral
Fellow, Univ. of California, Berkeley (1972-74). Univ. of British
Columbia (1974-76). Research Associate, Univ. of British Columbia(1976-77), Associate Research Endocrinologist. Univ. of California,
Berkeley (1977). Associate Professor. NIBB (1977-86). Professor.
NIBB (1986- ).

KEN-ICHI NAKA. Division of Sensory Processing. B. Sci., M . Sci.
& Ph.D. (biology, Kyushu Univ.). Visiting Fellow, NIH (1960-62),
Research Zoologist. UCLA (1962-63). Research Fellow. Univ. Cambridge (1963-64), Staff Member, Shionogi Research Lab. (1964-67).
Research Associate and Senior Research Associate, Calif. lnst.
Tech. (1968-77). Professor. Departments of Physiology and Biophysics and Ophthalmology, UTMB (1976-79), Professor. NIBB
(1979- ). H. W. Rand Fellow (1985).
YOSHIRO SHIMURA. Division of Cellular Communication. B. Sci.
& M. Sci., D. Sci. (botany, Kyoto Univ.). Ph.D. (microbiology andmolecular biology, Rutgers Univ.). Research Assistant, Institute ofMicrobiology, Rutgers Univ. (1958-63), Postdoctral Fellow, Department of Microbiology, The Johns Hopkins Univ. School of Medicine(1963-65), Instructor (1965-68). Research Fellow, Research Institutefor Microbiological Disease, Osaka Univ. (1968-69), Associate
Professor, Department of Biophysics. Kyoto Univ. (1969-85), Professor (1985- ), Adjunct Professor, NIBB (1986- ).
YOSHIAKI SUZUKI, Division of Cell Differentiation. B. Sci. & D.
Sci. (pharmaceut. sci., Univ. Tokyo). Staff Member, NIH of Japan(1959-71). Postdoctoral Fellow, Department of Embryology, Carnegie Institution of Washington (1968-71). Senior Staff Member, NIH
of Japan (1971-73), Staff Member. Department of Embryology,
Carnegie Institution of Washington (1973-78). Professor, NIBB
(1978- ).
IKUO TAKEUCHI. Division of Developmental Biology. B. Sci. & D.
Sci. (biology, Osaka Univ.). Ph.D. (biology, Princeton Univ.). Research Associate, Department of Biology, Osaka Univ. (1955-67).
Research Fellow. Department of Embryology, Carnegie Institution of
Washigton (1959-61). Professor. Department of Botany, Kyoto Univ.
(1967- ). Adjunct Professor. NIBB (1985- ).

49

TSUVOSHI UCHIDA, Divison of Cell Fusion. M .D. & Ph.D. (Osaka
Univ. Medical School). Research Associate of Research Institute forMicrobial Diseases. Osaka Univ. (1967-75). Research Fellow ofBiological Labs of Harvard Univ. (1970-72). Associate Professor of
Research Institute of Microbial Deseases, Osaka Univ. (1975-82).
Professor. Osaka Univ. (1982- ). Adjunct Associate Professor.
NIBB (1978-82). Adjunct Professor, NIBB (1985- ).

ASSOCIATE PROFESSORS
KOHJI HASUNUMA. Radioisotope Facility. B. Sci. (biology, Univ.
Tokyo). M . Sci. & D. Sci. (botany, Univ. Tokyo). Research Associate.
Univ. Tokyo (1971-79), Associate Professor, NIBB (1979- ).
SHIGERU ITOH. Division of Bioenergetics. B. Sci .. M. Sci .. & D.
Sci. (biochemistry, Univ. Tokyo) . Research Fellow. Department ofBiochemistry Univ. Bristol (1974-75). Research Associate. Department of Biology, Faculty of Sci .. Kyushu Univ. (1975-82). Associate
Professor, NIBB (1982- ).
MASAKI IWABUOHI. Division of Developmental Biology. B. Sci ..
M. Sci . & D. Sci. (biology, Hokkaido Univ.). Research Associate.
Hokkaido Univ. (1960-65). Research Associate, Hiroshima Univ.
(1965-66), Research Associate. Hokkaido Univ. (1967-79). Lecturer.
Hokkaido Univ. (1979-82). Associate Professor, Hokkaido Univ.
(1982- ). Adjunct Associate Professor. NIBB (1985- ).
TOSHIVUKI NAGATA, Division of Cell Mechanism . B. Sci . (biology, Univ. Tokyo). M. Sci. & D. Sci. (botany, Univ. Tokyo) . Research
Associate. Univ. Tokyo (1973-79). Alexander von Humboldt Fellow,
Max-Pianck-lnstitut. tor Biologie (1974-75). Research Associate,

50

Nagoya Univ. (1979-83). Associate Professor, NIBB (1983-).

KENZO NAKAMURA, Division of Cellular Communication. B. Agr.,
M . Agr. & D. Agr. (agricultural chemistry, Nagoya Univ.). Research
Fellow. Department of Biochemistry, State Univ. of New York at
Stony Brook (1976-79). Research Lecturer (1980-81 ). Researcher,
Mitsubishi-Kasei Institute of Life Science (1981-83), Associate
Professor. School of Agriculture, Nagoya Univ. (1983- ). Adjunct
Associate Professor. NIBB (1986- ).
SOICHI NAKAMURA. Department of Cell Proliferation. B. Sci .
(biochemistry, Saitama Univ.), M. Sci . (biology, Tokyo lnst. Tech .). D.
Sci. (biology, Osaka Univ.). Technical Staff, NIBB (1978-87). Associate Professor, General Education Division. Ryukyus Univ. (1987). Adjunct Associate Professor, NIBB (1988- ).
HIDEAKI NAKASHIMA, Division of Cellular Regulation . B. Sci. &M . Sci . (botany, Hiroshima Univ.). D. Sci . (botany, Nagoya Univ.).
Research Associate, Nagoya Univ. (1969-80). Research Fellow(Postdoctoral}, Univ. of California at Santa Cruz (1979-80). Associate
Professor, NIBB (1980- ).
MIKIO NISHIMURA. Division of Biological Regulation. B. Agr .. M .
Agr. & D. Agr. (agricultural chemistry, Nagoya Univ.). Research
Associate. Nagoya Univ. (1975-87). Postdoctoral Fellow, Univ. of
California at Santa Cruz (1977-79). Research Fellow, CENG at
Grenoble (1985). Associate Professor, Kobe Univ. (1987- ). Adjunct Associate Professor. NIBB (1988- ).
MASAHARU OGAWA, Division of Behavior and Neurobiology. B.
Sci. & M. Sci. (pharmaceutical sci. Osaka Univ.). Ph. D. (physiology,
Kyoto Univ. School of Medicine. Research Fellow, Kyoto Univ.
School of Medicine (1969-71 ), Instructor, Okayama Univ. School of
Pharmaceutical Sci . (1971-73). Instructor, Kyoto Univ. School

of Medicine (1977-79). Associate Professor. Kochi Med. Sch.
(1979- ). Adjunct Associate Professor. NIBB (1987- ).

KOHJI UENO. Division of Cell Differentiation. B. Sci .. M. Sci. &Ph. D. (pharmaceutical sciences. Univ. Tokyo). Research Associate,
Univ. Tokyo (1981-87). Associate Professor. NIBB (1987- ).
KENJI WATANABE. Division of Morphogeneis. B. Sci .. M . Sci. &D. Sci .. (biology, Univ. Kyoto). Research Associate. Department of
Anatomy. Kyoto Prefectural Univ. (1973-80). Associate Professor.
Department of Anatomy. Fukui Medical School (1980-86), Associate
Professor. NIBB (1986-- ).
MASARU YAMAIZUMI. Division of Cell Fusion . M .D. (medicine.
Kyoto Univ.) & Ph.D. (virology, Osaka Univ.). Research Associate.
Research Institute for Microbial Diseases, Osaka Univ. (1975-82).
Research Fellow. Department of Biology, Yale Univ. (1980). Research Associate. Institute for Molecular and Cellular Biology, Osaka
Univ. (1982). Associate Professor. Institute for Molecular andCellular Biology, Osaka Univ. (1983- ). Adjunct Associate Professor. NIBB (1983- ).

EIKI HIDA. Division of Sensory Processing . B. Sci. (mathematics.
Nagoya Univ.) & M . Sci. (biophysical engineering, Osaka Univ.).
Research Associate. NIBB (1979- ).
HIDETOSHI IIDA. Division of Cell Proliferation . B. Sci. (biology,
lbaraki Univ.). M . Sci . & D. Sci. (biophysics & biochemistry. Univ.
Tokyo) . Staff Member. The Tokyo Metropolitan Institute of Medical
Science (1978-85). Research Fellow. Dana-Farber Cancer Institute.
Harvard Medical School (1985-87) . Research Associate, NIBB
(1987- ).
MASAHIRO ISHIURA. Division of Cell Fusion . B. Sci . (biology,
Kanazawa Univ.). M . Sci. & D. Sci . (biology, Osaka Univ.). Research
Associate . Research Institute for Microbial Diseases. Osaka Univ.
(1977-79). Research Associate, NIBB (1979- ).
RVUJI KODAMA. Division of Morphogenesis. B. Sci. (biophysics
and biochemistry, Univ. Tokyo) . M . Sci. & D. Sci . (molecular biology,
Nagoya Univ.). Research Associate. NIBB (1983- ).
KENJI KOHNO. Division of Cell Fusion . B. Sci .. M . Sci. & D. Sci.
(microbiology, Univ. Tokyo) . Research Associate. NIBB (1980- ).

RESEARCH ASSOCIATESTAKAO KONDO. Division of Cellular Regulation . B. Sci .. M . Sci .
& D. Sci. (biology, Nagoya Univ.). Research Associate. NIBB
(1978- ).

KIVOKAZU AGATA. Division of Morphogenesis. B. Sci .. M. Sci.
& D. Sci. (biophysics. Kyoto Univ.). Research Associate. NIBB
(1983- ).

MAMORU MIMURO. Division of Bioenergetics. B. Sci .. M. Sci . &D. Sci . (biology, Univ. Tokyo). Postdoctoral Fellow. Ocean Res . lnst.
Univ. Tokyo (1978-80). Research Associate. NIBB (1980- ).

YOSHIO HAMADA. Tissue and Cell Culture Laboratory. B. Sci .
(biology, Osaka Univ.). M . Sci. & D. Sci . (biophysics. Kyoto Univ.).
Research Associate. NIBB (1979- ).

WATARU MITSUHASHI. Division of Biological Regulation . B. Sci ..
M . Sci. & D. Sci. (biology, Tokyo Metropolitan Univ.). Research
Associate. NIBB (1987- ).

51

IKUO NISHIDA, Division of Cellular Regulation . B. Sci. & M. Sci.
(chemistry, Nagoya Univ.). D. Sci. (coordinated science. Univ.
Tokyo), Research Associate, NIBB (1986- ).

Sci. (medical science. Keio Univ.). Research Associate, Keio Univ.
Med. School (1977-86). Research Fellow. Univ. Louis Pasteur deStrasbourg (1984--86). Rsearch Associate. NIBB (1986- ).

KAZUO OGAWA, Division of Cell Mechanism. B. Sci., M . Sci. &D. Sci. (biology, Tokyo Metropolitan Univ.). Junior Researcher. Univ.
Hawaii (1974-76). Research Fellow, Calif. lnst. Tech . (1976).
Research Associate, NIBB (1979- ).

MASAO TASAKA. Division of Developmental Biology. B. Sci.
(biology, Osaka Univ.), M. Sci. & D. Sci. (botany, Kyoto Univ.).
Postdoctoral Fellow. Kyoto Univ. (1980-83). York Univ. (1983-84).
Research Associate, NIBB (1985- ).

KAORI OHKI, Division of Bioenergetics. B. Agr. (agriculture, Kyoto
Univ.). M. Sci. & D. Sci . (botany, Kyoto Univ.). Technical Staff,
Ocean Res. lnst. Univ. Tokyo (1976-82), Research Associate, NIBB
(1982- ).

SATORU TOKUTOMI. Division of Biological Regulation. B. Sci .. M.
Sci. & D. Sci. (biophysics, Kyoto Univ.). Research Fellow, Kyoto
Univ. (1978-80). Research Associate, NIBB (1980- ).

KIVOTAKA OKADA, Division of Cellular Communication. B. Sci ..
M . Sci. & D. Sci. (biophysics, Kyoto Univ.). Research Associate,
Univ. Tokyo (1975-82, 84-86). Research fellow. Department ofBiochemistry and Molecular Biology, Harvard Univ. (1982-84) .
Research Associate. NIBB (1986- ).
HIROKO SAKAl, Division of Sensory Processing. B. L. A.. M . A.
(psychology, Int. Christian Univ.) & D. Sci. (zoology, Kyoto Univ.).
Research Associate, NIBB (1979- ).
TOSHIHARU SUZUKI, Division of Cell Differentiation. B. Sci.
(biology, Yamagata Univ.), M. Sci. & D. Sci. (biology, Nagoya Univ.).
Research Associate, NIBB (1985- ).
SHIGEHARU TAKIVA, Division of Cell Differentiation. B. Sci., M .
Sci. & D. Sci. (biology, Hokkaido Univ.), Research Fellow, Hokkaido
Univ. (1982-83), Technical Staff. NIBB (1983-84), Research Associate, (1985- ).
TAKAAKI TAMURA, E>ivision of behavior and Neurobiology. B. Sci.
(hygiene, Kitasato Univ.), M . Sci. (agriculture, Kagawa Univ.) & D.

52

MASAKATSU WATANABE, Large Spectrograph Laboratory. B.
Sci., M. Sci. & D. Sci. (biology, Univ. Tokyo) . Research Fellow, Univ.
Tokyo (1974-78), Research Associate. NIBB (1978- ).
KOTARO YAMAMOTO, Division of Biological Regulation. B. Sci ..
M. Sci. & D. Sci. (botany, Univ. Tokyo). Predoctoral Fellow,
Smithsonian Institution (1977-79). Research Associate. NIBB
(1980- ).
MICHIVASU VOSHIKUNI. Division of Reproductive Biology. B. Sci.
& M. Sci. (biochemistry, Shizuoka Univ.). D.Agr. (agricultural chemistry, Nagoya Univ.). Research Associate, NIBB (1988- ).

INSliiTUTE RESEARCHFELLOW
TEIICHI FURUICHI, Division of Behavior and Neurobiology. B. Sci.
(biology, Shinshu Univ.). M. Sci. & D. Sci. (biology, Tokyo Metropolitan Univ.). Visiting Graduate Student, State Univ. of New York at
Stony Brook (1983-86). Research Fellow, NIBB (1986- ).
MASANORI HIRANO, Division of Cellular Communication. B. Sci.
(Biology, Kyoto lnst. Tech.). M . Sci. & Ph. D. (biophysics. Kyoto
Univ.). Research Fellow. NIBB (1987- ).
YURIKO YAGAWA, Division of Cell Proliferation. B. Sci. & M. Sci.
(biology, Niigata Univ.) & D. Sci. (botany, Univ. Tokyo) . InstituteResearch Fellow. NIBB (1987- ).
TOSHINORI OZAKI. Division of Developmental Biology. B. Sci. , M .
Sci. & D. Sci. (botany, Hokkaido Univ.). Research Fellow. NIBB
(1988- ).

TECHNICAL DEPARTMENT
STAFF
Head
HACHIRO lrtONDA. Technical Department (Anjo Agricultural andForestry Higlil Sehool), Technical Staff, Nagoya Univ. (1953- 78),
Head, Technical Department. NIBB (1978- ).

Common Facility Group
Chief
HIROYUKI HATTORI. Center for Analytical Instruments. B. Sci.
(agriculture. Nagoya Univ.). Technical Officer. Nagoya Univ. (196579). Chief, Technical Staff, NIBB (1979- ).
KANAKO FUNADERA. Radioisotope Facility. B. Sci. (agriculture.
Kobe Univ.). Technical Staff. NIBB (1985- ).
KAZUHIKO FURUKAWA. Radioisotope Facility. B. Sci. (biology,
Toho Univ.). Technical Staff. NIBB (1979- ).
HIROKO KAJIURA, Center for Analytical Instruments. B. Sci .
(chemistry, Nagoya Univ.). Technical Staff, NIBB (1980- ).
MASAHIRO KAWAGUCHI. Tissue and Cell Culture Laboratory. B.
Sci. (chemistry, Shizuoka Univ.)•. Technical Staff. NIBB (1985- ).
HISASHI KOJIMA. Center for Analytical Instruments. B. Sci .
(industrial chemistry. Nagoya Institute of Technology), TechnicalStaff. NIBB (1982- ).
MAMORU KUBOTA. Large-Scale Spectrograph Laboratory. (radiocommunication, Miya Fishery High School). Technical Staff. NIBB
(1980- ).
VOSHIMI MATSUDA. Radioisotope Facility. B. Agr. (agriculture.
Shizuoka Univ.). Technical Staff. NIBB (1988- ).
CHIEKO NANBA. Experimental Farm. (agriculture. Shiga Prefecture
Junior Col.). Technical Staff, NIBB (1984- ).

53

MASAHIRO NASU, Laboratory Computer Facility. (Meinan Technical High School). Technical Staff, NIBB (1982- ).

MASAYO IWAKI. Department of Cell Biology. B. Sci. (chemistry.
Nara Women's Univ.). Technical Staff. NIBB (1985- ).

TOSHIKI OHKAWA. Laboratory Glassware Facility. (Shimizuminami High School). Technical Staff. NIBB (1984- ).

KAORU KATOU. Department of Developmental Biology. (agriculture. Shiga Prefecture Junior Col.). Technical Staff. NIBB (1985- ).

SATOMI ONO. Tissur and Cell Culture Laboratory. B. Sci. & M .
Sci. (chemistry, Nagoya Univ.). Technical Staff. NIBB (1988- ).

AKIO MURAKAMI. Department of Cell Biology. B. Sci. (biology,
Waseda Univ.). Research Fellow. Univ. Tokyo (1978-81). TechnicalStaff. NIBB (1981- ).

FUMIE SETO. Center for Analytical Instruments. B. Sci . (agriculture, Agricultures and Technology of Tokyo Univ.). Technical Staff.
NIBB (1987- ).
HISAYA UEMURA, Laboratory Glassware Facility. {pharmacology,
Kitasato Univ.). Technical Staff. NIBB (1985- ).

Research Support GroupSHINJI ADACHI. Department of Developmental Biology. B. Fish .
(biology, Hokkaido Univ.). Technical Staff. NIBB (1980- ).
YOHKO FUJIMURA, Department of Regulation Biology. B. Sci.
(biology, Tokyo Metropolitan Univ.). Technical Staff, NIBB (1980- ).
SACHIKO FUKADA. Department of Developmental Biology. B. Sci .
(biology, Tsukuba Univ.), Technical Staff. NIBB (1988- ).
SOH HIDAKA. Department of Regulation Biology. B. Sci . (biology,
Nagoya Univ.). Technical Staff. NIBB (1981- ).
SHOICHI HIGASHI. Department of Regulation Biology. B. Sci.
& M. Sci. (agriculture, Mie University), Technical Staff. NIBB
(1987- ).

54

MAYUMI NASU, Department of Developmental Biology. B. Sci.
(agriculture. Tokyo Univ. of Agriculture). Technical Staff. NIBB
(1985- ).
MIYUKI OHKUBO, Department of Developmental Biology. B. Sci .
(agriculture. Shizuoka Univ.). Tech nical Staff, NIBB (1986- ).
NORIKO SAKURAI, Department of Developmental Biology. B. Agr.
(agriculture, Meiji Univ.). Technical Staff. NIBB (1986- ).

ll!l

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