Gene Engineering Division
RIKEN BioResource Research Center

P‑1

RIKEN DNA BANKpTHK‑9a Clone lD

RDB07265

O Lot07265

A8F5a DNA Concentration

25 nanogram/microlitera Volume40 microliter

O Form

DNA solu‖ on in TE bufFera HostDH5 alpha

O Culture

LB medium

100 microgram/ml Ampicillin
a Antibiotics
QIAGEN QIAprep sp n Miniprep kn
a Purificationa Digestion by restriction enryme

07265
白雪 ぃ ∞¨¨(:b)Ｏ υШ

250 ng′ νve‖＞″コ Ｏ Ｃ コ(NEB#N3200L),︻工 Ｅ Ｏめl kb Plus DNA Ladder1003140311512=―̲―一■0一Electrophoresis

100 nanogram DNA per lane; 1% agarose gel , 'l x TAE Buffer:Restnc‖ 。n enzyme

Expected size of fragment

EcoRV

フ.8

2.7
5.6′ 2.2

Bann H I

5.1′

B9:II

kbp
kbp
kbp
kbp

O Confirmation of the insertion sequence
Please be sure to check our sequence analysis results before your request.
Sequence namePrimer lD

Primer name

Confirmed feature

Sequence - A

Pr01 00

Reverse-2

insert 5'

Sequence - B

Pr041 0

M1340

insert3'Sequence - C

Pr0564

Bkvirus Flinsert mid

Sequence - D
Sequence - E
Sequence - F
Sequence - G
Sequence - H

APPROVED BYI
hftp / / dna.hrcrlken jp

CGCapi20￨,p‖ mername A:reverse2
5LGCGGATAACAATTTCACACAGG‐駆燥ぃ,Pts 1480 to 12288 Pkl Loc:1457

KB 3500 POP7 BDTv3 mob

07265 07265 A8F5 3 Reverse2 G02 20 AB12407265 07265 A8F5 3 Reverse2」12「Xレ メ5バ′件え ア)レ t,

181

298

199

Spacing:10 96 Ptsノ Pane11 350」un 08,201802:33PM,JS丁」un 08,201801:51PM,JST!nst Mode1/Name 3500/3500 instrument

4,」 ソ/′ ア /

307

316

ＧＴ

ＧＡ
ＴＡ
Ａ

Ｔ
Ｔ

Ｔ

５２３ＧＴ

559

AICI嗜

４３・３442

７・２Ｇ

550

Ｔ

433

Ｔ

424

ＴＣ

415

541

Ｃ

172

289

406

532

ＣＧ163

280

397

523

ＧＧＧＴ

Electropherogram Data Page 1 oi 2658

Ｇ154

271

388

514

649

ACGTATATCATC CCＧ145

262379505

631８０２253

370

496

622

ＧＴ

136

361

487

613

TC TGTTCTTCTTTGTAAT TCT TGTCACGTTATACC
640

AGGGCCTAATACCGGAAACATCAGAATAATATTCCTGAATATAATTATAAAAGTTTATACCGCCATTTACAATTGTCCACG

604

Ｔ244GACCACGTCTTACAGTC CCGTACACGCCTACAAGTAAACGCAACATCCATTCAGGACCAGTTCT TTCATTACCACCTC127127235352469595Ｔ343460586ＴＣ̲̀ 1撃d(ガ [19030
Version 6.O HiSQV Bases:968
P!ate Name:20180608 dnabank,TT′ CC C(AAC C T TcCATC CCTG CAGG TCC AC TC TAG AこCC CAT T TCTC CGTTIIAC CAハ GCATTC TACCTC TG TTATACCATCTACCCCAG TTTTAil.HI
28 7̀計7364829110010910

NG:134A:249T:278 Ci309

メヽ硬ЬttBttrns
SノKB bcp1577GCCAAGGACTCTCTAAAAAATCTT3CTTCCCTTTCACCTACTTGTCTAACCAT TG

568

PAGTTTCC TCCAAA AATC TA478

TAAACTCTCCTCAATGTACAC TT TGTTCATTTCTTAAGTCCATTCTTTGTGTAACTTCTTCTATACTGTCAGCATCATCTATACTATAAGTATCGC CAAAATGTACACG226 )げTC TTCATATG CTTCApx,ム fcTTCTAnAACTTCTACTCCTCCTTTTATTAGTACTTTTGGCACTTCCACGGGGTCCTTTGGCTTTTTGGGACCTGCCCCTCCACACTCTCC1KB1 4.18

1090
54501090
54501090
54501090
54501090
54501090
545
Printed on:Fri Jun 08,201804:35PM,」 S丁676784

TCATCTG GGTT■685793694802703

811

ACAATTCCAAAGCCATCCCTCATTCCTCATAGCGC A G883

G A

T TT CG1045973

AA964

AAT C CC

ATG TTT

A C A

Ca92

CCTT A GG

A

982901

TACC A

1054

T A T A C

CC TA

TT TC」un 08,201802:33PM,JST

928

T A

1081

AAGG

TC CCC

1018

C

TC TA

775

1｀

10361874:」un 08,201801:51PM,JST766

865

9s5

C AA

ACAAC CTATACC C

1027

1090

A A

C AG TAG

CCElectropherogram Data Page 2 of 2

A Gs46856

757

lnst Modelノ Name 3500ノ 3500 instrument

Spacing:10 96 Ptsノ Pane11 350

9191009

CG T AC TTT G

GCに″αり71ち貿竺 ンゴTC A

74807265 07265 A8F5 3 Reverse2 G02 20 AB12407265 07265 A8F5 3 Reverse2
KB 3500 POP7 BDttv3 mob

910

T CC

1000

CCC T CA G1063

TAG

A C

xl 1

AAACTTTGTC ATCCCAATCAC TAハ838
847

739

Pts 1480 to 12288 Pkl Loc:1457730

Version 6 0 HiSQV Basesi 968
712

829

ACC CCTACAG TCG

721

Plate Name:20180608 dnabank
A AACT ATTTACACCACCAA ACAA AAT AT CA TAC TA991C

CC TA

C A CCCTTGACCCAAC GAACTAACACCAG TAACAG TTTC AA TTAAACCAG CAAACCCAC C AA TAC CCCCiC AGG ACC ACCAG C iATTA CAG

820

GCCTGGGAAATAGTACCAAACAAACAAGGACCCCAATCCCTACCATCAACCTATTCAATATTATTTACA

Cap:20

NG:134A:249丁 :278C:309

懲マ尊
釉合Bttrns
SノKB bcp

KB1418109054501090
54501090
54501090
54501090
5450
Printed oni Fri」 un 08,201804:35PM,JSTNG:94A:143丁 :145C:129賦蝠囃蝙合8輿 ms
SノKB bcp
CrGG C G1lr235

pttmername D :M13二 40‐127352244361i28:￨Ecθ RI

25337014507265 07265 A8F5 3 M13 ‐40 H02 23 ABi2407265 07265 A8F5 3 M13 ‐40463た55

172

460

577

469

478

595

487

604

496

TGTTCCTACATCAACAGGGTGTCGGGC

271

388

280

397

514

622

64

289

CTCTTTCTAAACCTGAT

505

613

406

181

73

298

190

532

640

82

307

424

199

649

」un 08,201801:51PM,JST」un 08,201802:33PM,JST

Spacingil1 05 Pts′ Pane11 350

217

il

Plate Name:20180608 dnabank
TAG CC:∫:GAGACAAt=ξ ]IGハlnst Mode!ノ Name 3500/3500 1nstrument

CCAζ

208

へGACC:&TTGI

559

GACCA TA:=2CCCTATTI∫325

TG TiCrl′ 、A Cr、 GACGTT′ ヽTTGGた

316

43〔550667

Electropherogram Data Page 1 of 2658

ACCCTGTATGTTTCAGCTCCTCATATTTGTCCCCTGTTTAC

523631541

AAATC TCCTG ATGTCCGAG GCTG TAAC,′

外4.ア ンにS//'2″ ぅィタ′163′

T TAC C GGCT TTAC TCTAAACCTAAGT GCTG AAAFT GACT聴 」はlT:1鴛 ぎ ■ :よ 里11:;:KB 3500 POP7 BDTv3 mob
ぞ154

TCCCCAAT TTG AATG

262379415

CCATCC TTAACCTTCATCCACGGTCACAAAAAGTACATGACCATCGTCGAGGGAACCCTATTCAACC CAG TAATTT CCACT TCTTTC CTGTTGGTCG

118

CCTTCCCTGTTiCACCACACCAACAATTCCCC568

rGTCACCAACiCAGC TACCACAC

ST586〕ハCAAAAACAATCCTTATCCAGTTCAGTCCTGGATTCCTCATCCCAGTAGAAATCAAAATACTAGGTATTTTCCCACTTTCACACCAGGGGAAAATGTTCCCCCAGTACTTCA343

G AAATCCACCC AGTCCTAATC AATTACAC CACAAAGTACCCAGAAGGTACTATAACTCCAAAAAACCCAACAGCCCAG TCCCACGTAATCAATACT〕

226

ヽTAACTA136

デ GttTTTCCCAGTmCGACGTTG私KB1418 Cap:23
G■ T
Gr /A r C-(q "^ :. Tt q--q--"it:1084
54201084
54201084
54201084
54201084
54201084
5420
Printed oni Fri Jun 08,201804:35PM,」

鱚 島Bttrns
S/NG:94A:143丁 :145C:129
KB bcpCap:23676784

TTTT T A

685

793

694

973

TCC G T T T T

982

1054

G802

703

901

ATATCATAACCTAAA TGC TG

1063

AA A AA C991

811

730

829

AC928

「マ739

838

AA TTC CAAA937」un 08,201801:51PM,JS丁」un 08,201802:33PM,JS丁:nst Mode!ノ Name 3500/3500 1nstrument:sAAT CC T T]∴

CCAAAA TC CTTTTAAACA

AA1027

A

1090

T G

TTT G T CG TT A TT TTTC

865

955

GGT￨81̀￨

1099

C Tο
ケ″
霞 ,十CG

GGGG

A A CC

1036

3ヒTTTGATc ccACAcA

Spacing:1l o5 Pts/Pane11 350

A919

CA

CCC A C TTTTT A A C C■0091018

AT T T856

Pts 1466 to 12305 Pkl Loc:1443

C G847

Plate Namei 20180608 dnabank
CCAATTT:∴TT

Vers:on 6 0721820

1000

G1072

AA

Electropherogram Data Page 2 of 2C

HiSQV Basesi 97007265 07265 A8F5 3 M13 ‑40 H02 23 AB:2407265 07265 A8F5 3 M13 ‐40
KB 3500 POP7 BDttv3 mob

712910ATATAT TC AC

T GG

C TTTTT G TA T AA G

● 1.cr,,GG

TC ACCT TATAAACAG GAG AACCCAC AC AGTCC ATCCC CACC CTATG TATCGTATCC AATCCCAGG TAC AAC AC G TCAC CG TG

A

AACACCTCTCCAACACAACAG TGGACAGGCCTTGCAACATATTTTAACATTCGCCTCAC AAAAACATCTGTAAキKB1 4.18108454201084542045

TCG

T G TACA TA TA C ATTTTAAA|1084____r_2

s4201084
54201084
54201084
542
0
Prlnted on:Fn」 un 08,201804:35PM,JSTSノ合8輿 ms

22610)(♭β5

CCAAGCCTGGAGGAAハ343568

460

p百mername c:BkVirus̲Fl469

136

28E"R7 37478253145487

154586595F」

翠t613

55172

6418129882CCAATAACGTC‐190

「si.

307

ATCTAGCCTGCAGGGTTTTACGG

406

415

199」un 21,201802:36PM,Js丁」un 21,201801:54PM,JS丁!nst Model/Name 3500/3500:nstrument

91

433

316

ム CAGG

523

640

532

541

208

100

325.

217

559

109/1442T CCA T G T:ょ

ATACTCATTCAT TGTAA

550 Nc。

667

Electropherogram Data Page 1 of 2658

334

lT TC cAC T TTGTAム G ATTCTTTTTTcハ424

TCCATCTAAATAATCTCTCACACTCTCTAAATTGTTT

631

649

ATCTATACCTACACCTAGTTCAAACGTTAGCCTTTccATGGGTAGGTT289

CCAAATTACCAG T,622￨￨

AAAAG TATAC AT AC T TA T C T CAG AAT C CAG C C TECISpacingi10 84 Pts/Pane11 350
TGPlate Name:20180621 dnabank16351473

HiSQV Basesi 972

Version 6 046271388505280

AV polyoo*aVir,-f -PN/l, fi Btrt???, t262379496

TCCTPts 1601 to 12324 Pkl Loci1578

KB 3500 POP7 BDTv3 mob07265 07265 A8F5 3 BKvirus Fl07265 07265 A8F5 3 BKvirus Fl D03 12 AB124

51 GGGA丁 丁CAGttGCTTGAttCCATG 3'9TC TTCTCT TG TCrTATcAA C ArTACAT T T CCCCA TC CATATAT TATAT T TCr￨｀￨127244361

TATTTGGGTTCTTTTGTTTAA352577ACATTTAAGGCTTTACCTCCACACAAATCTAATAACCCTCCAGCTACTGTTCTTTTTCCACTATCAATACGACCTTTAAATAACCAGTATCTTcTTTTAGGTACATTA1〕TCAACCCAAATCCTTTCATTCACCCCCTGTCCCTTTTACATCTTCAAAAACAACCATGTACTC370

TAAAAACTCTGAGTTTTGTAAGGATTTTCTTAAATATATTTTCGGCCTAAAATCTATCTCTCTTACAλ118

3CT TCCAT TCAACAA TTC TAG ATTGTATAT CT GTTGCAAAATCACC TACAC C CC T,lTCT(T TTTC TG

Cap:12

NG:45A:104丁 :146C:136

KB bcp

KB14181248
62401248
62401248
62401248
62401248
62401248
624Thu」 un 21,201804:23PM,JST金8輿 rnsCＣ784

T C GTCC

Cap:12し,676

̀代４９883

A973

892

T A C A T A C CT982

TCA TAC TT AAA G T G ATAハC T A
1054

901

811

910

C

Vers:on 6 0721:F′

TTCT GTTAC CATTT CTTC

730

HiSQV Basesi 972

Pts 1601 to 12324 Pkl Loc:1578

KB 3500 POP7 BDTv3 mob

07265 07265 A8F5 3 BKvirus Fl

1000

A

919

1009

A

928

ITCC GTTT

1018

TTTTTTT C TC AC A C TTTTTTT A C AC T (GA1081"負739

1『748946

1090T TTT1027

A C

TCTCG TCATATGAACGダCAοアう久妹リノ瑛
ご937

C ACCC」un 21,201801:54PM,JST」un 21,201802:36PM,JsT

inst Mode1/Name 3500/3500 1nstrument

757

766

TTA

TAC TCTTTTTT

1099

CC T C1036

TTA TT G

GTATC

955

TT CTT

775

T T

104 5

C T

1

Electropherogram Data Page 2 ol 2C

Spacing:lo 84 Pts′ Pane!1350
Plate Name:20180621 dnabank
AC CAA C ACCTC CCATATATTGTTCTAC TACACCATTTCcAT

07265 07265 A8F5 3 BKvirus Fl D03 12 AB124

712

ATATATCATTAAATCT820

ACACAATCCATTTTACGTACCAAACAGTCCACCC
703

991

TT1063A A A

CG TTC G TTC

A A TAAAAGCTAAAACTGTATCTACTC CTTG CTCACAAATACTTTTTTCATTTTTTACTTTTCTGC AAAG ATAGT AC CATTTTG CAAAAAT C c TTT685

S/NG:45 Al104丁 :146 Ci136
KB bcp

KB14181248
62401248
62401248
62401248
62401248
624
0Printed oni tthu」 un 21,201804:23PM,JST