Possibility of discriminating Japan wines from imported wines andother domestically produced wines
New labeling rules for domestic wines, introduced in October 2018, distinguish“Japan wines” which use domestically cultivated grapes only and “other domesticallyproduced wines” which use imported concentrated grape juice and/or imported wine.
In response to this labeling rule, we investigated the possibility of discriminating
Japan wines from other domestically produced wines or imported wines using thestable isotope and elemental analyses.
We analyzed 214 wines distributed in Japan: 82 Japan wines, 99 imported wines,and 33 other domestically produced wines. The oxygen stable isotopic composition1
significantly differed among the three groups with discrimination accuracy determinedby linear discrimination analysis2 being 88%, and discrimination accuracy similarly
determined for the concentrations of 18 inorganic elements3 being 88%. When theseanalytical methods were combined, and 19-factor linear discrimination analysis wasconducted, discrimination accuracy was improved to 93%. Although this experimentused commercially available wines, it was suggested that the analytical technique thatcombines the oxygen stable isotopic composition and the concentrations of inorganic
elements could be useful in discriminating Japan wines from other wines.
Possibility of discriminating Japan wines from other domestically produced and imported wines
by combining oxygen stable isotopic and elemental compositions was suggested.
1 Natural abundance ratio of 16O and 18O. The ratio of oxygen stable isotopes of water in wine is influenced by the cultivation
conditions of grapes and other factors.

2 Method for estimating the group that each sample belongs to.
3 Li, B, Na, Mg, Si, P, S, K, Ca, Mn, Co, Ni, Ga, Rb, Sr, Mo, Ba, Pb.

Possibility of discriminating Japan wines from imported wines and otherdomestically produced wines● New labeling rules for domestic wines (October 2018) clearly distinguish “Japan wines” from “other domestically produced wines”.
● The possibility of distinguishing these wines was investigated by physicochemical analysis (stable isotopes, inorganic elements).
● Commercially available wines were used: 82 Japan, 99 imported, and 33 other domestically produced wines.

Ratio of oxygen stable
isotopes of water in wine (%)

Ratio of stable isotopes
10Ratio of stable isotopes +inorganic elements
Imported wines
Domestically producedwines*※Japan wines
Japan wines (traceable samples)

5

Canonical 20‐5‐10 ０

Domestically
produced wines*

Japanwines

Importedwines

 Ratio of oxygen stable isotopes significantly differed among the threegroups. Discrimination accuracy determined by linear discrimination
was 88%.

Inorganic elements
Japan wines
Canonical 2

Imported wines
Domesticallyproduced wines*

Canonical 1

 Accuracy of discriminating the three group determined by linear
discrimination analysis, based on the concentration of 18 inorganic elements(Li, B, Na, Mg, Si, P, S, K, Ca, Mn, Co, Ni, Ga, Rb, Sr, Mo, Ba, Pb), was 88%

Canonical 1
Discrimination point plots when the ratio of oxygen stable isotopes andthe concentrations of 18 inorganic element were combined When the ratio of oxygen stable isotopes and theconcentrations of inorganic elements were combined,discrimination accuracy was improved to over 93%.
 Possibility was suggested that the combination of the ratio ofoxygen stable isotopes and concentrations of inorganicelements is useful for discriminating Japan wines from otherwines.
 More reliable methods will be investigated.
*Other domestically produced wines