Gene Engineering Division
RIKEN BioResource Research Center

P‑1n RIKEN DNA BANK cione name:pCALNL5RI"=a clone lD

RDB 01862

O Lot

19245

a DNA Concentration

25 nanogram/microlitera volume40 microliter

O Form

DNA solution in TE bu「 era Hosta Culture

DH5 alpha

O Antibiotics'100 microg ram/ml Ampicillin

81la

LB mediurn

QIAGEN QlAprep Spin Miniprep kit
a Purificationa Digestion by restriction enryme0■ 86 2︻０250 ng′ v′ ellＸ

Ｈ０３ ∽″コ Ｏ Ｃ コ(NEB#N3200L),

こ ０∞︻工 ´l kb PIus DNA Ladde「(lb)30≡≡

40,01.210︺ 一一 一一一■●︼︼﹈︼¨●︼■●■●︺ 一一

20

1.5

OS01

: 100 nanogram DNA per lane ; 1% agarose gel , 1 x TAE Buffer
Expected size of fragment
Restriction enzyme
6.1
kbp
SwaI
:

Electrophoresis

XhoIBanlHI

4.9′ ■.2

kbp

5.8′ 0.3

kbp
kbp

O Confirmation of the insertion sequence
Please be sure to check our sequence analysis results before your request.
Sequence namePrimerlD

Primer name

Confirmed feature

Sequence - A

国□口日園■CAG Rl

CAG pro,lox P,NeoR 5'Sequence - BPЮ 066

pCA-bgter

lox P,SV40 pA,NeoR 3'Sequence - C

P「 0731SV40pro_ori_F

SV40 pro̲ori,SV40 pA,ori

Sequence - D
Sequence - E
Sequence - F
０ノSequence - G
Sequence - H

APPROVED BY:
http://dna. brc. riken. jp

KB 3500 P(Э P7 BDTv3.rnob

01862 19245 Bl!a3CAG Rl

Spacing:10.74 Ptsノ Pane!1350

Sep 17,202102:28PM,JST

131

471

581

361

こハ G PrO

141

481

591

371

151

381

Version 6.O
51

HiSQV Basesi 659

171

401

61

181

411

501

N∞ 7611

511

621

521

63171531641

8■

こハQP"

311421

201431‐91321

Nヽ DR

1 541

I11

卜41吹 1

Sep 17,202102:01PM,JS丁CN21――――――――引
￨111

Plate Narne:20210917 mix

101

221

CCCTCTCCTAACCATGT451

341

TAACTTCGTATACC

31441

671

561551ATTCAACAACATCCATTCCACCCAGGTTCTCCGGCCGCTTGG

191

GTC CG GCC CCG GCAGG A,G GiA■ TC CCCG GGG,GGGCCTTCC TCCGTCCCCCCC CCCCCG TCCCCTTCTCCC

161391

T CC CATTTGATCT GATCAAG AGACAGG ATCACCATCGTTTCC

491601lnst Mode:ノ Nanne 3500′ 3500:nstrument

バBttB醐 繋msPts 1700 to 8704 1Pkl Loc:1668

3101862 19245 Blla 3 CAG Rl E01 05 AB108

primer name /へSノ NG:30A:17丁 :20 Ci22i CAG̲Rl5'TTGTCCCAAAITCTGTGCGGA3'2141KB.bcp

Cap:5351

TACATTATACCAAGTTATQox?

461571

rGTCCGGTCCCCTGAATCAACTGCAGGACCAGGCANCGCCCCTATCG TCCCTGGNCACCACNGGCGTNCCTT

Electropherogram Data Page 1 of 2

G CCCACCTG TCCT CG ACGTTTGGACAGGCTATTCGCCTATGACTCCCCACAACAGACAATCCCCTCCTCTCATCCCGCCGTGTTCC13GCTGTCAGCCCANGGGCGCCCGGTTCTTTTTGTCNAGiN気ぃ。
ITCATCCCTTCTTCTTTTTCCTACAGCTCCTG GGCAACGTGCTGGTTATTGTCCTGTCTCATCATTTTGGCAAAC AATTC ATTAATTCGAG CC A
,1241251261271281291301121

TCTCCACCCTCCGGGCTGTCCGCGGGGGGACCCCTGCCTTCGGGGGGGACCGGGCAGGCCGGGGTTCCCCTTCTCGCGTCTCACCGGCGCCT11

NNNNNNNNN Nさ NNNNヽ N NNNlNNN NNNN GG CC CG GGGNNKB l.41.8

106853401068534010685340106853401068534010685340
Printed oni Fri Sep 1 7,202102:57PM,JSTCap:6

NG:37A:36T:37C:33

バB合 8馴里msSノ

31

2丁11η
うmpl κフ・・

primer name 3 :pCA― bgter

21

31251

slCvr^!

361

591

371

GC GGCG T CC GT‐
581

261

・、GC・

381

CG‐・NG
601

271

Sep 17,202102:01PM,JST

lnst Model/Narne 3500/3500 1nstrument

Sep 17,202102:28PM,JS丁Spacingi10.78 Pts/Pane!1350

61

181

71

χOえ ?

n191

81

C TT CG TATAAT GTATGCTATACGAAGTTAT

291

301

311

91

餡にI

431

101

221

TCC G C TC̲T

441

331

211

̀

341

671

GNN NGC

561

CC GAT

451

TCGGATT321NωCGGG N N CC

Electropherogram Data Page 1 of 2661

CAAGA ACTC GT CAAC AACCC CATACA
541551421

53164

TTCiPts 1774 to 8704,IPkl Loc:1742281

411

TCハ TCCAGCCGCCGTCCCGGAAAACGATTCCGAAGCCCAACCTTTCATACAACCCG

401521631

CCTCTT NN NNN T TCr‐

111

Plate NarTlei 2021091 7 mix

391

621

NCGGT C.G CCCFTTcccccccl̲‐611

20

CCA G GC CATT G GCCACACCA C C CACCACCT T CT GATA GGCAGCCTC CACCTG AC CAGTCA■171←一一i」夏 :G IT‐rメち。

JlVersion 6.O

161

TTTAAATC GTACCCAG CGACT⊆151

Gニ ム エユLQA■̲ ̲̲141

Eり RI

HiSQV Basesi 64101862̲19245̲311a̲3̲pCA― bgter̲F01̲06̲AB10801862̲19245̲Blla̲3̲pCA‐ bgter
KB 3500 POP7 BDTv3.:Tlob

51ATTAGCCAGAAGTCAGATGCTC 31131…・C̲T GGGGTCCCCGAAGAACTCCAGCATCAGATCCCCCCCC TGGAGG■・・ … …
・su+o'ntpolg4;l・〕 ■ GTT:… TC・一 一 )ιいTCGGG‐〕CGGTGGAATCCAAATCTCGTGATCGCACGTTGGCCGTCGCTTCGTCGGTCATTTCCAACCCCAGAGTCCCGC461471481491501

571

3CT CC G511CATACATTGATGAGTTTGGACAAACCACAACTACAATCCAGTCAAAAAAATCCTTTATTTGTCAAATTTCTGATGCTATTCCTTTATTTGTAACCATTATAACCTGCAATAAA5Qι I]TCCCG GGTACCCAG CTC■

NN NNさ :Nヽ :N TNNさ ささNNNlNN N NNN Nさ NNヽ N Cl'C Gl.G GT

KB.bcpKB l.4.1.8

1154
577
01154
577
01154
577
01154
577
01154
577

1154
577
Printed on:Fri Sep 17,202102:57PM,JS丁NG:156A:107T:137C:13〔

バB金 8Jttms
Sノ

Cap:7

GCGT

571

ヽCCTCCC

411

primer name c′

21:SV40pro̲ori̲F1

おOY｀ i481

591

491

601

inst Mode!ノ Nanne 3500ノ 3500!nstrunnent

Sep 17,202102:01PM,JST
Sep 17,202102:28PM,JST511

521

631

71531

641

91

1()1441331661

9.4Hfl561̀〕111

CCTCC341:NTAT671

451:L:L:L」

Srr+o?U)

GTiTCTT=,TCiTCTCTcGニ551

̲̲̲̲̲̲̲̲翌上̲̲̲̲̲̲」541

TTCNGG AACCGT GGC CCTTTC TCNTAElectropherogram Data Page 1 ol 2

GACCCTCAAGTCACAGGTGCCCAAACCCGACACCACTATAAACATACCAGGCGTTTCCCCCTChN

621

ュL⊆

ACTTIGTTTATT GCAC C TTATAA TC C

KB 3500 POP7 BDTv3.mob

ACCTP!ate NalTle:20210917 mix

81

Spacingi10.78 Pts′ Pane11 350

61201

HiSQV Bases:676

501

611431GAGGCCGTTTCCGTATTCGCCGCTCTTCCCCTTCCTCCCTCACTCiCTCGCTCCGCTCGGTCGTTCGGCTCCGCCGACCCGTATCAI・291
3()1311321261271281Pい )191

Pts 1729 to 8704 1Pkl Loc:1697

Version 6.O

SM,ro Va/oti

51

AAGTACT GACCiGGCTTTTTTGIGAGCCCTA(3GCTTTTGCAA

3101862̲19245̲Blla̲3̲SV40pro̲ori̲F̲G01̲07̲AB10801862̲19245̲Blla̲3̲SV40pro̲ori̲F￨

i41

5L TTCTCCGCCCCATGGCTGACTAAT‐581

TCG TCCCC TCTCCTGTTCCCACCC TCCCGCT TACC GGATACCT(3TC CG CC TTTCTCCC471

TTCCATAGCCTCCGCCCCCCTGACCACCATCACAAAAATCCTCACTCAAACGCGGTAATACGGTTATCCACAGAATCACCGGATAACCCACCAAAGAACATGTCACCAAAAGGCCAGCAAAAGCCCACGAACCGTAAAAAGGCCGCCTTCCT351361371381391
401411421

TTAATGAATCCCCCAACCCGCGGGG■
231241251

̀ヽTACAAATAAAGCAATACCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCAT TCTAGTTGTCCTTTGTCCAAACTCATCAATI
121131141151
1611711811

Nさ さNヽ NNCさ N lNlN NNG CCT CN N CCTC TC iNCT「KB.bcp
KB l.4.1.8

129464701294
647012946470129464701294647012946470
Printed on:FrilSep 1 7,202102:57PM,JS丁