ER255/455 SystemBundle
User Manual V1.02 Feb 2012
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Page 1EDAQ ER255 User Manual EDID-00523-MAD

Contents
INTRODUCTION ............................................................................................................................................. 3
ER255 Configuration – Schematic diagram ............................................................................................... 3
ER255 Configuration ‐ Pictorial View ........................................................................................................ 4
ER455 Configuration – Schematic diagram .............................................................................................. 5
ER455 Configuration – Pictorial View ....................................................................................................... 6
Trigger Connection .................................................................................................................................... 7
System Cable Summary............................................................................................................................. 8ER225 C4D Data System Cables ............................................................................................................ 8
ER230 HV Sequencer............................................................................................................................. 8ET225 Micronit Chip Electrophoresis Platform ..................................................................................... 8Page 2

EDAQ ER255 User Manual EDID-00523-MAD

ER255/455 System bundle User Manual
INTRODUCTION
This manual is intended to allow the user to interconnect the components provided in this bundle. It provides
detailed information on how the system should be connected. Please read this manual carefully prior tobeginning operation.
The ER255 System Bundle consists of the following components:
 1 x ER230 HV Sequencer for ER255 or 2 x ER230 for the ER455 bundle ER225 C4D data system ET225 Chip Platform Microchips, standard solutions and Cables
Please note: separate User manuals are provided for each component of the bundle – please read thesecarefully in association with this document.
Install each element of this system and become familiar with its operation before connecting all systemelements together. Always install software first and then hardware hardware.

ER255 Configuration – Schematic diagram
Mains Input

ER225 Front

ER 225 Front Panel

ER225 Rear Panel

Head CableGround
CableET225
Platform

Trigger
Optional

USB

Interlockcable

USB

ER230 Rear

ER230
Front

M1

M2

Out 1

Out 2

ARM/DISARMPB
12V Powerpack

Page 3EDAQ ER255 User Manual EDID-00523-MAD

ER255 Configuration - Pictorial View

This cable usedwith 45mm chips
GROUND
Connection
MUST BE
CONNECTED

External I/O
Connector
USB
Connection

METER HVINPUT

GroundConnection

HV OUTPUT(HSV)This casble usedwith 90mm chips

INTERLOCK
BNC

ARM/DISARMPBIMPORTANT: The interlock cable needs tobe fully inserted into the ET255 platform.
This cable has a “flat” KEY THAT SHOULD
BE POSITIONED TOWARDS THE BOTTOM
WHEN INSERTING THE CABLEFLAT KEY

Page 4

Interlock cable detail

EDAQ ER255 User Manual EDID-00523-MAD

ER455 Configuration – Schematic diagram

Mains Input

ER225 Rear Panel

ER225 Front

ER225 Front PanelER225
C4D Data
SystemHead cable

GroundCable

ET225
Platform

Trigger
Optional

USB

USB

ER230
MASTERER230 Rear

ER230
Front

M1

M2

Out 1

I2C
Cable

Out 2

Interlockcable
BNC T-Piece

12V Powerpack

ER230
Front

ER230 Rear

ER230
SLAVE

ON/OFFPushbutton

M1

M2

Out 1

Out 2

ARM/DISARMPB

12V Powerpack

NOTE1 : The ER230 unit which is connected to USB becomes the MASTER – the other unit becomes theSLAVE and communicates via the I2C cable.
NOTE 2: The grey I2C cable connects between the MASTER I2C Output and the SLAVE IEC INPUTNOTE 3: A BNC T-Piece is used to connect the interlock signal between the Master and Slave.

Page 5EDAQ ER255 User Manual EDID-00523-MAD

ER455 Configuration – Pictorial View
Cable

I2C Cable connected
between MASTEROUTPUT and SLAVEINPUTMASTERUNIT
USB cableconnected

Triggercable

BNC
T- Piece

Page 6EDAQ ER255 User Manual EDID-00523-MAD

Trigger Connection
It is convenient to be able to control the data acquisition cycle of the system by ensuring that it occurs only inthat period where useful data will be expected to be output.
This can be done manually by pressing “start” in the data recording software PowerChrom or Chart.
A preferred method is to let the HV sequence control this process. By connecting the CTL 1 line to the ER225
Trigger input – as shown below you will be able to initiate the start of a data recording cycle at a convenienttime.
For example: a pulse could be programmed in the Sequencer table to start the data recording say 30 seconds
after the separation phase has begun. This will ensure that the data recording will not contain the largetransients which may occur when high voltages are being switched during the loading and separation phases.
For details on how to program the sequencer table refer to the ER230 User manual.

TRIGGER Cable Detail

ER 225 REAR PANEL

ER230 REAR PANEL

Page 7EDAQ ER255 User Manual EDID-00523-MAD

System Cable Summary
This section describes the cables provided with the various components of the system together with theiruses.

ER225 C4D Data System CablesPower Cord: a region specific mains cable is provided to connect power to the ER225 unit
USB Cable: USB cable provided for connection to the computer
Instrument connector (green 20 way plug) used to connect control signals – See ER225 fordetails
Trigger cable: Twin wire cable used to connect a trigger signal to or from an ER225 to an ER230
HV Sequencer. Either unit is capable of generating or accepting a trigger signal. The preferred
method is to trigger the ER225 Data system some time after the ER230 has completed the load
cycle and has begun its separation cycle.

In the ER455 example given above the following connections have been made to allow the ER230
Unit to start data recording:
ER225 Trigger + PIN 1 (red wire) connected to ER230 CTL1 + PIN 13
ER225 Trigger – PIN 2 (Blue or white wire connected to ER230 CTL1 – PIN 14

ER230 HV Sequencer







Universal Mains adaptor – provides 12V power to the ER230 system
Power Cord: a region specific mains cable is provided to connect power to the ER225 unit
Ground/Earth cable 4mm stackable to 4 mm stackable cable.
USB Cable: USB cable provided for connection to the computer
Instrument connector (green 20 way plug) used to connect control signals – See ER225 fordetails
I2C cable to connect two ER230 systems in a Master/Slave arrangement
BNC T- Piece. Allows connection of the of INTERLOCK signal from the Master to the Slave.
BNC to BNC cable to connect interlocks when 2 HV sequencers are used in a Master/Slavearrangement.

ET225 Micronit Chip Electrophoresis Platform
Page 8

Ground Cable – 2mm to 4mm green cable provides ground link between the ET225 and theER230
Interlock Cable DIN to HTMI connector provides connection between the ET225 and the ER225
data system. Please Note this is not a standard HDMI and should not be connected to yourcomputer – only to the Chip platform.
High voltage cables are fixed to the chip platform and are not provided separately.

EDAQ ER255 User Manual EDID-00523-MAD