LOCUS
SOURCEORGANISMFEATURES
BASE COUNT
ORIGINRDB 6083 pRSV_s-FLAG
preliminary; circular DNA; SYN; 5190 BP.
Cloning vector
Artificial sequences; Cloning vehicles.
Location/Qualifiers
1200 A1324 C1351 G
1315 T

BglII
1 GACGGATCGGGAGATCTCCCGATCCCCTATGGTCGACTCTCAGTACAATCTGCTCTGATG
61 CCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCG
121 CGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGC|5' end of RSV promoter
181 TTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTATCTGAG
241 GGGACTAGGGTGTGTTTAGGCGAAAAGCGGGGCTTCGGTTGTACGCGGTTAGGAGTCCCC
301 TCAGGATATAGTAGTTTCGCTTTTGCATAGGGAGGGGGAAATGTAGTCTTATGCAATACA
361 CTTGTAGTCTTGCAACATGGTAACGATGAGTTAGCAACATGCCTTACAAGGAGAGAAAAA
421 GCACCGTGCATGCCGATTGGTGGAAGTAAGGTGGTACGATCGTGCCTTATTAGGAAGGCA
EcoRI*481 ACAGACAGGTCTGACATGGATTGGACGAACCACTGAATTCCGCATTGCAGAGATAATTGT
3'end of RSV541 ATTTAAGTGCCTAGCTCGATACAATAAACGCCATTTGACCATTCACCACATTGGTGTGCA
promoter|EcoRI* NcoI*NotI
601 CCTCCAAGCTGAATTCACCATGGACTACAAGGACGATGACGATAAGGCGGCCGCTCGAGC
AlaGluPheThrMETAspTyrLysAspAspAspAspLysAlaAlaAlaArgAlasingle FLAG tag* They are not unique.
XbaI|5' end of BGH poly(A) signal
661 ATGCATCTAGAGCTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTG
CysIle***721 TTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCT781 AATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTG
3' end of BGH poly(A) signal|841 GGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATG
901 CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCGAGG GGGGATCCCC
961 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG
1021 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA
1081 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT CCCTTTAGGG TTCCGATTTA
1141 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC
1201 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG
1261 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT
1321 AAGGGATTTT GGGGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA
www.brc.riken.jp/lab/dna/1

1381 ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTAAAT ATTTGCTTAT ACAATCTTCC
1441 TGTTTTTGGG GCTTTTCTGA TTATCAACCG GGGTGGGTAC CGAGCTCGAA TTCTGTGGAA1501 TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GGCAGGCAGA AGTATGCAAA
1561 GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA
1621 GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC
1681 CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT
1741 TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG
1801 GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGG ATATCCATTT
1861 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
1921 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA
1981 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT2041 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT
2101 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG
2161 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
2221 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC
2281 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA
2341 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG
2401 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC
2461 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG
2521 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA2581 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG
2641 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC
2701 TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC
2761 CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT
2821 GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC
2881 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT2941 TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT3001 CCCGTCGACC TCGAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT3061 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT
3121 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG
3181 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG
3241 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
3301 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT3361 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC3421 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC3481 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA3541 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT3601 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG3661 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
3721 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
3781 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
3841 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG
3901 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC3961 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT4021 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT4081 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA4141 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA4201 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
4261 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT4321 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
4381 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT4441 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT4501 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC4561 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC4621 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT4681 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACTwww.brc.riken.jp/lab/dna/24741 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC4801 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT4861 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG4921 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT4981 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA5041 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT5101 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC5161 ACATTTCCCC GAAAAGTGCC ACCTGACGTC//www.brc.riken.jp/lab/dna/3

RIKEN DNA BankRIKEN BioResource Center

RDB2136 S-HA-pRc/CMV
T7 promoter
HindIIITTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTTCCATGGCCTACCCCTACGACGTGCCCGAC
AlaSerMetAlaTyrProTyrAspValProAsp
single HA tag
NotI
XbaI ApaI <- SP6 promoter
TACGCCGCGGCCGCTCGAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGA
TyrAlaAlaAlaAlaArgAlaCysIle***RDB2137 S-T7-pRc/CMV
T7 promoter
HindIIITTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTTCCATGGCCAGCATGACCGGCGGCCAGCAG
AlaSerMetAlaSerMetThrGlyGlyGlnGlnsingle T7 tag
NotIXbaI ApaI <- SP6 promoter
ATGGGCGCGGCCGCTCGAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGA
MetGlyAlaAlaAlaArgAlaCysIle***RDB2138 D-T7-pRc/CMV
T7 promoter
TTATCGAAATTAATACGACTCACTATAGGGAGACCC
HindIII
AAGCTTCCATGGCCAGCATGACCGGCGGCCAGCAGATGGGCGCGGCCATGGCCAGCATGACCGGCGGCCAGCAGATGGGCAlaSerMetAlaSerMetThrGlyGlyGlnGlnMetGlyAlaAlaMetAlaSerMetThrGlyGlyGlnGlnMetGly
double T7 tag
NotIXbaI ApaI <- SP6 promoter
GCGGCCGCTCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGA
AlaAlaAlaLerAsnGlyProIleLeu***

RDB2139 D-HA-pRc/CMV
T7 promoter
TTATCGAAATTAATACGACTCACTATAGGGAGACCCHindIII
AAGCTTCCATGGCCTACCCCTACGACGTGCCCGACTACGCCGCGGCCTACCCCTACGACGTGCCCGACTAC
AlaSerMetAlaTyrProTyrAspValProAspTyrAlaAlaAlaTyrProTyrAspValProAspTyr
double HA tagNotIXbaI ApaI <- SP6 promoter
GCGGCCGCTCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGA
AlaAlaAlaLerAsnGlyProIleLeu***www.brc.riken.jp/lab/dna/RIKEN DNA BankRIKEN BioResource Center

RDB2140 S-Myc-pRc/CMV
T7 promoter
HindIIITTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTTCCATGGAGCAGAAGCTGATCAGCGAGGAG
AlaSerMetGluGlnLysLeuIleSerGluGlu
single Myc tag
NotIXbaI ApaI <- SP6 promoter
GACCTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGAAspLeuAlaAlaAlaArgAlaCysIle***RDB2141 D-Myc-pRc/CMV
T7 promoter
TTATCGAAATTAATACGACTCACTATAGGGAGACCC
HindIII
AAGCTTCCATGGAGCAGAAGCTGATCAGCGAGGAGGACCTGGCGGCCGAGCAGAAGCTGATAAGCGAGGAGGACCTG
AlaSerMetGluGlnLysLeuIleSerGluGluAspLeuAlaAlaGluGlnLysLeuIleSerGluGluAspLeu
double Myc tag
NotI
XbaI ApaI <- SP6 promoter
GCGGCCGCTCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGA
AlaAlaAlaLerAsnGlyProIleLeu***www.brc.riken.jp/lab/dna/RIKEN DNA BankRIKEN BioResource Center

RDB 5954 pCMV_S-Myc
T7 promoter
TTATCGAAATTAATACGACTCACTATAGGGAGACCC

EcoRI* NcoI*NcoI*
AAGCTGAATTCACCATGGCAGCTTCCATGGAGCAGAAGCTGATCAGCGAGGAGGACCTG
AlaGluPheThrMETAlaAlaSerMetGluGlnLysLeuIleSerGluGluAspLeu
single Myc tag
NotIXbaI ApaI <- SP6 promoter
GCGGCCGCTCGAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGA
AlaAlaAlaArgAlaCysIle***RDB 5955 pCMV_D-Myc
T7 promoter
EcoRI* NcoI*TTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGAATTCACCATGGCAAlaGluPheThrMETAla
NcoI*
GCTTCCATGGAGCAGAAGCTGATCAGCGAGGAGGACCTGGCGGCCGAGCAGAAGCTGATAAGCGAGGAGGACCTG
AlaSerMetGluGlnLysLeuIleSerGluGluAspLeuAlaAlaGluGlnLysLeuIleSerGluGluAspLeu
double Myc tag
NotI
XbaI ApaI <- SP6 promoter
GCGGCCGCTCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGA
AlaAlaAlaLerAsnGlyProIleLeu***

RDB 5956 pCMV_S-FLAG
T7 promoter
TTATCGAAATTAATACGACTCACTATAGGGAGACCCEcoRI* NcoI*
AAGCTGAATTCACCATGGACTACAAGGACGATGACGATAAGAlaGluPheThrMETAspTyrLysAspAspAspAspLys
single FLAG tag
NotIXbaI ApaI <- SP6 promoter
GCGGCCGCTCGAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAlaAlaAlaArgAlaCysIle****They are not unique.

www.brc.riken.jp/lab/dna/RIKEN DNA BankRIKEN BioResource Center

RDB 6083 pRSV_S-FLAG
3'end of RSV promoter|
EcoRI* NcoI*ACATTGGTGTGCACCTCCAAGCTGAATTCACCATGGACTACAAGGACGATGACGATAAG
AlaGluPheThrMETAspTyrLysAspAspAspAspLys
single FLAG tag
NotIXbaI|5' end of BGH poly(A) signal
GCGGCCGCTCGAGCATGCATCTAGAGCTCGCTGATCAGCCTCGACTGTGCC
AlaAlaAlaArgAlaCysIle**** They are not unique.

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