Antibodies for the Study of the Nucleus.

Active Motif is committed to providing
the highest quality antibodies for studying
chromatin and the biology of the nucleus.
We specialize in manufacturing antibodies
against histones, histone modifications andchromatin proteins, many of which havebeen validated for use in chromatin IP (ChIP)
and immunofluorescence (IF). Each antibody

that we make is rigorously tested to ensure
you won’t waste your precious time andresearch dollars on antibodies that don’t
perform as specified.
At Active Motif, we believe that you should
always repeat your experiments, but notbecause your reagents failed.

Our Antibody Commitment
Quality First
We’d rather fail our project thansacrifice quality.
Highly Characterized
All of our antibodies are stringently
tested under multiple conditions.
Controlled Process
We manufacture and test our own
antibodies, rather than re-selling.
Consistent
We go to great lengths to minimize
lot-to-lot variability.
FIGURE 1:
Immunofluorescence with Active Motif antibodies.
HeLa cells stained with Aurora B antibody (1:200 dilution). One of the cells isundergoing mitosis and is in anaphase, at which point Aurora B (red) can bevisualized in the spindle midzone. Top Left: Aurora B pAb (Cat. No. 39261).
Top Right: alpha Tubulin mAb (Cat. No. 39527). Bottom Left: DAPI nuclear stain.
Bottom Right: all 3 images merged.

www.activemotif.com

NORTH AMERICA 877 222 9543

EUROPE +32 (0)2 653 0001

JAPAN +81 (0)3 5225 3638

Extensive selection. Smart design.
Histones and Histone Modification Antibodies
Because histone post-translational modifications areimportant regulators of genome function, Active Motif isdeveloping antibodies for all widely studied, biologically
relevant modification sites. Each antibody is rigorously
screened for specificity and tested to verify the applications it works well in, such as ChIP, Westerns & IF.
Complete details: www.activemotif.com/histoneabs

Antibody Development Process
At Active Motif, antibody development and testing isa very important and tightly controlled process, withmany steps along the way. From the design of theimmunogen to the specificity screening and technique validation, our years of expertise in antibody
development ensure that only the highest quality
antibodies are offered for use in your research.

Transcription Factor & Cell Signaling Antibodies
To complement our assays for studying the activation ofimportant signaling pathways, Active Motif offers a wide
variety of antibodies to transcription factors and cellsignaling proteins. As with our other antibodies, these arecharacterized for specificity and application tested.
Complete details: www.activemotif.com/tfabs

ChIP-validated Antibodies
Chromatin immunoprecipitation is an extremely challenging technique, so only antibodies of the highest quality
will do. For an antibody to work in chromatin IP, it must
be of high titer and it must be very specific, with nodetection of non-target proteins. Most importantly, it
must be able to recognize the target protein in its native
chromatin-associated context after fixation.
Complete details: www.activemotif.com/chipabs

FIGURE 2:
Chromatin immunoprecipitation of methylated histone H3.
ChIP was performed with Histone H3K4me2 pAb (Cat. No. 39141)
using chromatin prepared from mouse embryonic stem cells (leftlane) or mouse erythroleukemia cells (right lane). PCR primers
specific for the b-globin gene were used to amplify a 210 base pairregion of the promoter following isolation of DNA.
6.0

Antibodies to Chromatin Modifiers
There are many important non-histone chromatin proteins, from histone modifying enzymes like acetyltransferases, deacetylases and methyltransferases to chromatin-binding proteins like HP1, which read the “histonecode”. Because these proteins regulate chromatin structure and function, Active Motif offers high-quality antibodies to these targets so you can study their roles.
Complete details: www.activemotif.com/chromabs

Fluorescent and HRP Secondary Antibodies
To get the most from your use of our primaries, Active
Motif offers high-quality secondary antibodies conjugated to a wide variety of fluorescent dyes and to HRP.
Complete details: www.activemotif.com/secondary

Fold Enrichment

5.0
4.0
3.0
2.0
1.0
0.0

PABC1

GAPDH

MyoD

Control IgG

FIGURE 3:
Histone H3 acetyl Lys14 pAb used in ChIP and analyzed by RT-qPCR.
Chromatin IP was performed with the ChIP-IT™ Express Kit (Cat.
No. 53008) and Ready-to-ChIP HeLa Chromatin (Cat. No. 53015)
using 5 µg of Histone H3K14ac pAb (Cat. No. 39697) or rabbit IgG
as a negative control. Real time, quantitative PCR (RT-qPCR) was
then performed using primer pairs specific for the indicated genes.
Data are presented as Fold Enrichment of the ChIP antibody signal
versus that of the negative control using the ddCT method.

For a list of all antibodies offered by Active Motif, please visit us at www.activemotif.com/abs
www.activemotif.com

NORTH AMERICA 877 222 9543

EUROPE +32 (0)2 653 0001

JAPAN +81 (0)3 5225 3638

Rigorous testing ensures performance.
Immunogen selection

Technique validation

Immunogens are selected to decrease thelikelihood of cross-reactivity with related
proteins and to maximize detection of theprotein in its native context. Immunogens
for modification-specific antibodies are
selected to ensure that the antibody
recognizes the modified version of theprotein only.

Each antibody is validated for use intechniques such as ChIP (Figures 2 & 3) andIF (Figures 1, 4 & 8) so you can be confident
when using them in your experiments.
FIGURE 4:
Immunofluorescence testing.
HeLa cells stained with alpha Tubulin mAb in red(Cat. No. 39527, panels A and D), Histone H4monomethyl Lys20 pAb in green (Cat. No. 39175,
panels B and D) and DAPI nuclear stain in blue
(panels C and D). Note the strong nuclear staining andabsence of cytoplasmic staining in panel B, which is to
be expected of an antibody to a histone modification.

Antibodies to histone modifications
that exist in budding yeast (S. cerevisiae)
can be further screened for specificity
by testing strains of yeast that containa point mutation at the position of themodified amino acid (Figure 7).

1
me
K9
H3

K9

me

H3

K4

me

H3

K4

me
K4

H3

K4

H3

H3

3

Additional specificity testing

Western blotting is performed to verify
the antibody recognizes the correct
molecular weight protein and does notcross-react with other proteins. Commonlyused chemical treatments that stimulate
a modification (e.g. HDAC inhibitors andacetylation) are used to better detect themodification (Figure 6).

2Western blot

1

Specificity screening
The first test performed on every antibody is dot blot analysis, which ensures
its specificity for the desired protein or
modification (Figure 5). Antibodies that do
not exhibit a greater than 25-fold selectivity for the desired modification are failed.

250
50
FIGURE 7:
Mutational analysis of yeast modifications.
Histone H4K12ac pAb (Cat. No. 39165) was tested byWestern blot using extracts from wild-type yeast or
yeast containing a mutated histone H4 gene.

e3
7m

e2

K2

7m

H3

K2

e1
7m

H3

K2
H3

K2

7

3
me

K9

H3

H3

H3

K9

me

2

10

250
50

Lane 1: Yeast with wild-type histone H4.
Lane 2: H4 with the amino-terminal tail deleted.
Lane 3: H4 with an arginine instead of lysine 5.
Lane 4: H4 with an arginine instead of lysine 8.
Lane 5: H4 with an arginine instead of lysine 12.
Lane 6: H4 with an arginine instead of lysine 16.
Lane 7: H4 with arginines instead of lysines 5, 8 & 12.

10
FIGURE 5:
Dot blot analysis confirms specificity.
Ten, 50 and 250 picomole aliquots of synthetic peptides corresponding to histone H3 that is unmodified, mono-, di- or trimethylated at Lys4 (Lanes 1-4),
Lys9 (Lanes 5-8) or Lys27 (Lanes 9-12) were spottedand probed with Histone H3K27me3 pAb (Cat. No.
39155), confirming the antibody’s specificity for histone H3 trimethyl Lys27 (Lane 12). No cross-reactivity
is observed for the unmodified Lys27 peptide (Lane9) and only a hint of signal is observed at the 250
picomole spots for mono- (Lane 10) and dimethyl
Lys27 (Lane 11).

FIGURE 6:
Western blot confirming size and modification.
Histone H2BK120ac pAb (Cat. No. 39119) was tested
by Western blot. No reactivity is observed onrecombinant human histone H2B (Lane 1, 200 ng
protein), as it is not acetylated. Lanes 2 and 3 contain
5 μg each of HeLa cell acid extract. Cells that weretreated with a histone deacetylase inhibitor (sodium
butyrate) prior to extraction have an increased signal(Lane 3), which is to be expected for an antibody toa site of acetylation.

Note the absence of reactivity in yeast extracts inwhich lysine 12 is either missing or mutated(Lanes 2, 5 & 7).

For a list of all antibodies offered by Active Motif, please visit us at www.activemotif.com/abs
www.activemotif.com

NORTH AMERICA 877 222 9543

EUROPE +32 (0)2 653 0001

JAPAN +81 (0)3 5225 3638

High-quality Secondary Antibody Conjugates.
Improved primary detection
To provide you with a variety of choices for
detecting your primary antibodies, Active
Motif offers a variety of anti-mouse & antirabbit secondary antibodies that have been
conjugated to a broad line of high-quality
fluorescent molecules (Table 1), as well as HRP.

Dye

Absorption

Emissione L/(mol-cm)

Stokes Shift

Chromeo™ 488

498 nm524 nm73,00026 nm

Chromeo™ 494

489 nm624 nm55,000135 nmChromeo™ 505

514 nm

530 nm70,00016 nm

Chromeo™ 546

550 nm

567 nm

98,80017 nm

Verified for a variety of applications

Chromeo™ 642

647 nm

666 nm180,000

19 nm

Active Motif’s secondary conjugates are
ideal for use in various applications including
fluorescent microscopy and flow cytometry,
where they have been shown to work withhigh efficiency and specificity under multiple
fixation conditions. Of note, due to a verylarge Stokes shift of 134 nm (Figure 9), our red
Chromeo™ 494 conjugates are ideal for multiplexing with 488-excitable, green fluorescentdyes (Figure 8). And, our Chromeo 488, ATTO
647N and ATTO 655 conjugates are certified
and recommended by Leica Microsystems
for use with its powerful STED (STimulated
Emission Depletion) microscopes.

ATTO 532 (GSD)

534 nm

560 nm115,000

26 nm

ATTO 594

601 nm

627 nm

120,000

26 nm

ATTO 647N (STED/GSD)

644 nm

669 nm150,000

25 nm

ATTO 655 (STED)

663 nm

684 nm

125,000

21 nm

Rhodamine 6G (GSD)

508 nm

558 nm116,000

50 nm

Optimized conjugation method

TA B L E 1 :Properties of Active Motif’s fluorescent conjugated secondary antibodies.

Histone H2AX phospho
Ser139 pAbalpha Tubulin mAb++
Chromeo™ 494
Chromeo™ 488
Goat anti-rabbit IgG Goat anti-mouse IgG

DAPI Nuclear Stain

All 3 images merged

Next to the spectral properties of the dye
and the quality of the secondary antibody,the quality of a fluorescent conjugate is
influenced by the dye-to protein ratio, theconjugation method and its purity. All Active
Motif Secondary Antibody Conjugates havebeen prepared by an optimized conjugation
protocol that makes the fluorescence brighter while lowering the background, ensuringthat you get the best results possible.
1

FIGURE 8:
Concurrent staining using Chromeo 488 and Chromeo 494 secondary antibody conjugates in untreated andetoposide-treated HeLa cells.
HeLa cells were either left untreated (row 1), or treated with 100 µM etoposide for 6 hours (row 2), prior to
fixation with methanol. The histone variant H2AX was then stained with Histone H2AX phospho Ser139 rabbitpAb (Cat. No. 39117) and Chromeo 494 goat anti-rabbit IgG (Cat. No. 15042), while tubulin was visualized usingalpha Tubulin mouse mAb (Cat. No. 39527) and Chromeo 488 goat anti-mouse IgG (Cat. No. 15031). The nuclei
were stained using DAPI nuclear stain, and the three separate images were then merged.

Chromeo™ 494 absorption
Chromeo™ 494 emission

0.8

0.6

0.4

0.2

0
350

400

450

500

550

600

650

700

750

800

850

Wavelength (nm)

FIGURE 9:
Absorption and emission spectra of Chromeo 494.
The very large Stokes shift of Chromeo 494 (134 nm)
makes it an ideal partner for multiplexing because while
it is excited by the same sources used for fluorescein,
FITC, Chromeo 488 and other 488 nm-excitable dyes,
its red emission is separated completely from the green
fluorescence emitted by these other 488-excitable dyes

Active Motif Antibodies (as of January 13, 2014)
For an up-to-date list of all available antibodies and for more comprehensive information on each antibody, including data, images and downloadable Technical Data Sheets, please
visit our website at www.activemotif.com/abs. Or, if you click any antibody name in the table below, you will be linked directly to its page on our website. To see our Fluorescentand HRP Secondary Antibody Conjugates, please go to the last pages of this antibody list or visit www.activemotif.com/secondary.

NEW

Antibody

Immunogen

Reactivity

Applications

3-Methylcytosine (3-mC) antibody (pAb)

Raised against 3-methylcytidine conjugated to KLH. (Rabbit serum)

H, M, WRDB

3-Methylcytosine (3-mC) antibody (pAb)

Raised against 3-methylcytidine conjugated to KLH. (Purified rabbit IgG)

H, M, WRDB

5-Carboxylcytosine (5-caC) antibody (pAb)

Raised against 5-carboxylcytidine conjugated to KLH. (Rabbit serum)

NS

DB, IF

5-Carboxylcytosine (5-caC) antibody (pAb)

Raised against 5-carboxylcytidine conjugated to KLH. (Purified rabbit IgG)

NS

DB

5-Formylcytosine (5-fC) antibody (pAb)

Raised against 5-formylcytidine conjugated to KLH. (Rabbit serum)

NS

DB, IF

5-Formylcytosine (5-fC) antibody (pAb)

Raised against 5-formylcytidine conjugated to KLH. (Purified rabbit IgG)

NS

DB

5-Hydroxymethylcytosine (5-hmC) antibody(mAb) (Clone 59.1)

Raised against 5-Hydroxymethylcytidine conjugated to KLH. (Purified mouse IgG)

H, M, WR

DB, MeDIP

5-Hydroxymethylcytosine (5-hmC) antibody (pAb) Raised against 5-Hydroxymethylcytidine conjugated to KLH. (Rabbit serum)

H, M, WR

DB, IF, IHC, MeDIP

5-Hydroxymethylcytosine (5-hmC) antibody (pAb) Raised against 5-Hydroxymethylcytidine conjugated to KLH. (Purified rabbit IgG)

H, M, WR

DB, MeDIP

5-Methylcytosine (5-mC) antibody (mAb)(Clone 33D3)

Recognizes the modified 5-methylcytidine base in plant and vertebrate DNA.
(Purified mouse IgG)

NS

DB, FC, IHC,
IP, MeDIP

5-Methylcytosine (5-mC) antibody (pAb)

Raised against 5-methylcytidine conjugated to KLH. (Purified rabbit IgG)

NS

DB, IP, MeDIP

5-Methylcytosine (5-mC) antibody (mAb)(Clone A1)

Raised against 5-methylcytidine conjugated to KLH. (Purified mouse IgG)

NS

DB, MeDIP

Raised against 6-methyladenosine conjugated to KLH. (Purified rabbit IgG)

NS

DB

Bridging Antibody for Mouse IgG
A20 antibody (mAb) (Clone 59A426)

Raised against purified mouse IgG.
Raised against full-length human A20.

M
H

ChIP, IP
IP, WB

Acf1 antibody (pAb)

Raised against the recombinant, C-terminal half of Drosophila Acf1.

Dr

IF, IP, WB

NEW 6-Methyladenosine (6-meA) antibody (pAb)

FormatCatalog No.

100 µl10 µl100 µg10 µg100 µl10 µl100 µg10 µg100 µl10 µl100 µg10 µg100 µg10 µg100 µl10 µl100 µg
10 µg

61179
61180
61111
61112
61225
61226
61229
61230
61223
61224
61227
61228
39999
40000
39769
39770
39791
39792

50 µg

39649

100 µg10 µg100 µg10 µg100 µg10 µg
500 µg100 µg100 µl
10 µl

61255
61256
61479
61480
61495
61496
53017
40901
39721
39722

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

1

Antibody

Immunogen

Reactivity

Applications

Ago1/2/3 antibody (mAb) (Clone 2A8)

Raised against a recombinant protein with amino acids 47-879 of human Ago2.

H

ChIP, IF, IHC, IP, WB

Ago1 antibody (mAb) (Clone 4B8)

Raised against a peptide containing the N-terminal region of human Ago1.

H

IP, WB

Ago2 antibody (mAb) (Clone 1B1-E2H5)

Raised against a recombinant protein with amino acids 1-148 of human Ago2.

H

IF, IP, WB

Ago3 antibody (mAb) (Clone 4B1-F6)

Raised against a recombinant protein with amino acids 1-175 of human Ago3.

H

IF, IP

Ago4 antibody (mAb) (Clone 1B7-G11)

Raised against a recombinant protein with amino acids 1-164 of human Ago4.

H

IP, WB

Ago4 antibody (pAb)

Raised against a peptide containing the N-terminal region of human Ago4.

H

WB

AIB1 / SRC-3 antibody (pAb)

Raised against a peptide derived from human AIB1 / SRC-3.

H

ChIP, WBAIB3 antibody (pAb)

Raised against a recombinant protein with amino acids 2-146 of human AIB3.

H, M

IHC, IP, WB

AID antibody (mAb) (Clone 328.8b)

Raised against amino acids 178-198 of human AID.

H, M

IF, IP, WB

Aiolos antibody (pAb)

Raised against a GST-tagged, C-terminal half of mouse Aiolos.

M

IF, IP, WB

Aiolos antibody (mAb) (Clone 8B2)

Raised against the N-terminal half of mouse Aiolos.

M

ChIP, WB

AIRE antibody (pAb)

Raised against a peptide to the C-terminal region of human AIRE.

H

WB

AKT1 phospho Ser473 antibody (mAb)(Clone 104A282)

Raised against a peptide with phospho-serine 473 of human AKT1.

H, M

WB

alpha Tubulin antibody (mAb) (Clone 5-B-1-2)

Raised against total protein purified from sea urchin sperm axonemes.

H, M, WR

IF, WB

AML-1/Runx1 antibody (pAb)
AML-2/Runx3 antibody (pAb)
AML-3/Runx2 antibody (pAb)

Raised against amino acids 231-245 of human AML-1/Runx1.
Raised against full-length human AML-2/Runx3.
Raised against amino acids 333-348 of human AML-3/Runx2.

H, M
H
H

Androgen Receptor antibody (pAb)

Raised against a peptide of the N-terminal region of human Androgen Receptor.

H, M

AP-2 antibody (pAb)
APE antibody (pAb)
APEXL2 antibody (pAb)

Raised against amino acids 120-134 of human AP-2.
Peptide mixture of amino acids 36-52 and 216-232 of human APE.
Raised against a peptide corresponding to human APEXL2.

H
H
H

WB
EMSA, WB
EMSA
ChIP, ChCh,
ChSeq, IP, WB
WB
WB
WB

FormatCatalog No.

100 µg10 µg100 µg10 µg100 µg10 µg100 µg10 µg100 µg10 µg100 µl10 µl100 µl10 µl100 µl10 µl100 µg10 µg200 µl10 µl100 µg10 µg100 µl
10 µl

39937
39938
61071
61072
39853
39854
39787
39788
39855
39856
39813
39814
39797
39798
39835
39836
39885
39886
39293
39294
39657
39658
61139
61140

100 µg40902

200 µl10 µl100 µl100 µl100 µl100 µl10 µl100 µg100 µg200 µl

39527
39528
39000
39301
39302
39781
39782
39001
39200
39201

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

2

Antibody

Immunogen

Reactivity

Applications

FormatCatalog No.

APOBEC2 antibody (pAb)

Raised against a peptide of the N-terminal region of human APOBEC2.

H

WB

WB

100 µl10 µl200 µl10 µl200 µl10 µl100 µg10 µg100 µg10 µg100 µl10 µl100 µg

39985
39986
39231
39232
39099
39100
61359
61360
61205
61206
61369
61370
40977

Dimethyl-Arginine, asymmetric antibody (pAb)

Raised against a peptide with dimethyl-arginine (asymmetric).

NS

IF, WB

ASH2L antibody (pAb)

Raised against full-length recombinant human ASH2 protein.

H, WR

IF, IP, WB

ASH2L antibody (mAb) (Clone 4C5)

Raised against a recombinant protein containing the N-terminal region ofhuman ASH2L.

H

IHC, WBASPM / MCPH5 antibody (pAb)

Raised against a peptide to the C-terminal region of human ASPM / MCPH5.

H

IF

ATAD2 antibody (pAb)

Raised against a peptide to the C-terminal region of human ATAD2.

H

WB

ATF-2 antibody (pAb)
ATF-2 phospho Thr71 antibody (mAb)(Clone 103C411)
ATF-6 antibody (mAb) (Clone 70B1413)
ATM phospho Ser1981 antibody (mAb)(Clone 10H11.E.12)

Raised against amino acids 13-27 of human ATF-2.

H

Raised against a peptide with phospho-threonine 71 of human ATF-2.

H

WB

100 µg40975

Raised against a partial protein of amino acids 1-273 of human ATF-6.

H

IF, WB

Raised against a peptide with phospho-serine 1981 of human ATM.

H, M

IF

ATRX antibody (pAb)

Raised against a peptide with the C-terminus of human ATRX.

H

WB

Atxn7 antibody (mAb) (Clone 3SCA-1C1)

Raised a recombinant protein containing amino acids 1-229 of human Atxn7.

H, M

IF, IHC, WB

Atxn7l3 antibody (mAb) (Clone 2ATX-2B1)

Raised a peptide containing amino acids 278-296 of human Atxn7l3.

H, M

IP, WB

Aurora B antibody (pAb)

Raised against a peptide with the C-terminus of human Aurora B.

H, M, WR

IF, WB

Raised against a peptide to the C-terminal region of human BAP1.

M

WB

Bcl3 antibody (pAb)

Raised against a C-terminal peptide of human Bcl3.

H

WB

Bcl6 antibody (mAb) (Clone IG191E)

Raised against full-length recombinant human Bcl6 protein.

H

IHC, WB

Bcl10 antibody (mAb) (Clone 151)

Raised against full-length recombinant human Bcl10 protein.

H

WB

BCL11A / CTIP1 antibody (pAb)

Raised against a peptide of the N-terminal region of human BCL11A / CTIP1.

H

WB

BCL11A antibody (mAb) (Clone BCL11A/123)

Raised against a recombinant protein containing amino acids 637-835 of
BCL11A-XL (B-cell lymphoma/leukemia 11A extra long form).

H

IF, IHC, WB

100 µg200 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg200 µl10 µl100 µl10 µl100 µl10 µl100 µg10 µg200 µg10 µg100 µl10 µl100 µg
10 µg

40962
39529
39530
61049
61050
61347
61348
61339
61340
39261
39262
61501
61502
39761
39762
61193
61194
39393
39394
61051
61052
61231
61232

NEW BAP1 antibody (pAb)

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

3

Antibody

Immunogen

Reactivity

Applications

beta Tubulin antibody (mAb) (Clone 2-28-33)

Raised against sarkosyl-resistant filaments from Strongylocentrotus purpuratus(sea urchin) sperm axonemes.

H, WR

IHC, WB

BLIMP1 / PRDM1 antibody (pAb)

Raised against a peptide of the N-terminal half of human BLIMP1 / PRDM1.

HWB

BLM antibody (pAb)

Raised against a peptide of the N-terminal region of human BLM.

H

WB

BMI-1 antibody (mAb) (Clone AF27)

Raised against full-length recombinant mouse BMI-1 protein.

H, M

ChIP, IPBMI-1 antibody (mAb) (Clone DC9)

Raised against full-length recombinant mouse BMI-1 protein.

H, M

IF, WB

Boris / CTCFL antibody (pAb)

Raised against a peptide of the N-terminal region of human Boris.

H

WB

NEW BRD3 antibody (pAb)

Raised against a peptide of the C-terminal of human BRD3.

H, M

WB

BRD4 antibody (pAb)

Raised against a peptide of the C-terminal of human BRD4.

H

ChIP, IP, WB

BRD8 / SMAP2 antibody (pAb)

Raised against a peptide of the N-terminal region of human BRD8 / SMAP2.

H

WBBrdU antibody (mAb) (Clone Bu20a)

Raised against Bromodeoxyuridine conjugated to BSA.

NS

IHC

Bub1 antibody (mAb) (Clone 14H5)

Raised against amino acids 281-419 of human Bub1.

H, M

IF, WB

BubR1 antibody (mAb) (Clone 8G1)

Raised against amino acids 1-350 of human BubR1.

H, M, WR

IF, WB

CAF-1 p60 antibody (mAb) (Clone 24)

Raised against full-length CAF-1 protein purified from 293 cells.

H, M

IF, IP, WB

CAF-1 p150 antibody (mAb) (Clone 1)

Raised against full-length CAF-1 protein purified from 293 cells.

H

IF, IP, WB

CARM1 antibody (pAb)

Raised against a peptide to the C-terminus of mouse CARM1.

H, M, RWB

Caspase-3 antibody (mAb) (Clone 31A1067)
Caspase-7 antibody (mAb) (Clone 25B204.1)
Caspase-8 antibody (mAb) (Clone 90A992)
Caspase-9 antibody (pAb) (Pro-form)
Caspase-14 antibody (mAb) (Clone 70A1426)

Raised against 6xHis-tagged, full-length human Caspase-3 protein.
Raised against a recombinant human Caspase-7 protein.
Raised against amino acids 217-222 of human Caspase-8.
Raised against amino acids 299 -318 of human Pro-Caspase-9.
Raised against amino acids 2-18 of mouse Caspase-14.

H, M, R
H
H
H
H, M

WB
WB
FC, IHC, WB
WB
WB

H

WB

NEW beta-Catenin antibody (pAb)

Raised against a peptide within the N-terminal region of human beta-Catenin.

FormatCatalog No.

100 µg10 µg100 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg100 µl10 µl100 µl10 µl100 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg100 µg10 µg100 µg10 µg100 µg10 µg200 µl10 µl100 µg100 µg100 µg100 µg100 µg100 µl
10 µl

61157
61158
61053
61054
61055
61056
39993
39994
61089
61090
39851
39852
61489
61490
39909
39910
61007
61008
61273
61274
39735
39736
39747
39748
39995
39996
39997
39998
39251
39252
40924
40929
40930
40931
40932
61487
61488

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

4

Antibody

Immunogen

CBX8 antibody (pAb)
NEW CDK8 antibody (pAb)

CDT1 antibody (mAb)
NEW CENP-A phospho Ser18 antibody (pAb)

Reactivity

Applications

Raised against a peptide to the C-terminal region of human CBX8(Chromobox protein homolog 8).

H

WB

Raised against a peptide to the C-terminal region of human CDK8.

H

WB

H

IHC, IP, WB

H, WR

DB, IF, WB

Raised against a recombinant protein containing amino acids 177-557 ofmouse CDT1.
Raised against a peptide containing phosphorylated serine 16 and serine 18 ofhuman CENP-A.

CENP-B antibody (pAb)

Raised against a peptide to the C-terminal region of human CENP-B.

HIF

CENP-B antibody (pAb)

Raised against recombinant protein containing the C-terminal region of human
CENP-B.

H

WB

CENP-E antibody (mAb) (Clone 1H12)

Raised against full-length human CENP-E.

H, M

IF

CGBP antibody (pAb)

Peptide mixture of amino acids 73-90 and 641-656 of human CGBP.

H, M

WB

CHD1 antibody (pAb)

Raised against amino acids 251-467 of human CHD1.

H

ChIP, WB

CHD1 antibody (mAb) (Clone 2F11)

Raised against a peptide to the C-terminus of mouse CHD1.

H, M

IF, WB

CHD2 antibody (pAb)

Raised against a peptide to the C-terminus of human CHD2.

H, WR

WB

Raised against a peptide containing amino acids 865-885 of Drosophila CHD3.

Dr

IF, IP, WB

CID antibody (pAb)

Raised against recombinant CID protein from Drosophila.

DrWB

CID antibody (pAb)

Raised against recombinant CID protein from Drosophila.

Dr

IF

Copine I antibody (pAb)

Raised against amino acids 519-532 of human Copine I.

H

WB

CoREST antibody (pAb)

Raised against a peptide to the C-terminal region of human CoREST.

H

WBCREB antibody (mAb) (Clone 82B514)

Raised against a peptide corresponding to human CREB.

HWBCTBP antibody (pAb)

Raised against a peptide to the C-terminal region of human CTBP.

H

WB

CTBP2 antibody (pAb)

Raised against a peptide to the C-terminal region of human CTBP2. This
antibody recognizes both isoform 1 and 2

H

WB

CTCF antibody (pAb)

Raised against a peptide within the C-terminal region of human CTCF.

H, M

ChIP, ChCh, ChSeq,
IP, WBNEW CHD3 antibody (mAb) (Clone 7A11)

FormatCatalog No.

100 µl10 µl100 µl10 µl100 µg10 µg100 µl10 µl100 µg10 µg100 µl10 µl200 µg10 µg200 µl100 µl10 µl100 µg10 µg200 µl10 µl100 µg10 µg100 µl10 µl100 µl10 µl200 µl10 µl100 µl10 µl100 µg100 µl10 µl100 µl10 µl100 µl
10 µl

61237
61238
61481
61482
61039
61040
61483
61484
61183
61184
61287
61288
39619
39620
39203
39729
39730
39989
39990
39363
39364
61469
61470
39713
39714
39719
39720
39101
39102
39955
39956
40961
39847
39848
61261
61262
61311
61312

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

5

Antibody

Immunogen

Reactivity

Applications

CtIP antibody (mAb) (Clone 14-1)

Raised against a recombinant protein containing amino acids 620-897 of
human CtIP.

H

IF, IP, WB

Raised against a peptide to the N-terminal region of human CUL4B.

H

WB

Cyk-4 phospho Ser170 antibody (pAb)

Raised against a peptide with phospho-serine 170 of human Cyk-4.

H

DB, IF, WB

D4-GDI antibody (mAb) (Clone 97A1015)
DAP-3 antibody (mAb) (Clone 42C617)
DAP-5 antibody (mAb) (Clone 39C534)

Raised against the Fas-induced cleavage site of human D4-GDI.
Raised against amino acids 51-66 of human DAP-3.
Raised against amino acids 796-812 of human DAP-5.

H
H, M
H, M

DAX-1 / NR0B1 antibody (mAb) (Clone 1DA-2F4)

Raised against a peptide with amino acids 135-166 of human DAX-1 / NR0B1.

H, M, R

DcR3 antibody (mAb) (Clone 37A565)

Raised against full-length human DcR3.

H

IP, WBWBWB
ELISA, ICC, IF,
IHC, IP, WB
WB

DDB1 antibody (pAb)

Raised against a peptide to the C-terminal region of human DDB1.

H

WB

Ddit3 antibody (mAb) (Clone 9C8)

Raised against full-length recombinant mouse Ddit3 protein.

M

IP, WB

H, M

WB

H, M

ChIP, IHC, IP, WB

HWB

NEW CUL4B antibody (pAb)

Dicer antibody (mAb) (Clone N167/7)
DNMT1 antibody (mAb) (Clone 60B1220.1)
DNMT1 antibody (pAb)

Raised against a recombinant protein containing amino acids 1638-1899 ofmouse Dicer.
Raised against amino acids 637-651 of human DNMT1.
Raised against a recombinant protein containing amino acids 1-110 of human
DNMT1.

NEW DNMT1 antibody (mAb) (Clone 2C1)

Raised against full-length recombinant human DNMT1 protein.

H

ChIP, IP, WB

NEW DNMT1 monomethyl Lys142 antibody (pAb)

Raised against a peptide with monomethyl-lysine 142 of human DNMT1.

H

DB, WB

Raised against amino acids 39-53 and 361-376 of mouse DNMT2.
Raised against full-length mouse DNMT3A.

H, M
H, M

WB
ChIP, IF, IHC, WB

H, M, WRWB

DNMT2 antibody (pAb)
DNMT3A antibody (mAb) (Clone 64B1446)
NEW DNMT3A antibody (mAb) (Clone 6A4)

Raised against a peptide with amino acids 200-215 of human DNMT3A.

DNMT3A antibody (pAb)

Raised against a recombinant protein containing amino acids 1-247 of mouse
DNMT3A.

MWB

DNMT3A dimethyl Lys44 antibody (pAb)

Raised against a peptide with dimethyl-lysine 44 of mouse DNMT3A.

H, M

WB

DNMT3B antibody (mAb) (Clone 52A1018)

Raised against full-length mouse DNMT3B.
Raised against a recombinant protein containing amino acids 1-218 of mouse
DNMT3B.

H, M

ChIP, IF, IP, WB

M

WB

DNMT3B antibody (pAb)

FormatCatalog No.

100 µg10 µg100 µl10 µl200 µl10 µl100 µg100 µg100 µg100 µg10 µg100 µg100 µl10 µl100 µg10 µg100 µg10 µg100 µg100 µl10 µl100 µg10 µg100 µl10 µl100 µg100 µg100 µg10 µg100 µl10 µl100 µl10 µl100 µg100 µl
10 µl

61141
61142
61445
61446
39265
39266
40941
40963
40964
39983
39984
40933
61127
61128
61349
61350
39817
39818
39204
39905
39906
61467
61468
61497
61498
39205
39206
61477
61478
39897
39898
61323
61324
39207
39899
39900

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

6

Antibody

Reactivity

Applications

Raised against a peptide with amino acids 383-396 of human DNMT3B.

H

WB

DNMT3L antibody (pAb)

Raised against full-length recombinant human DNMT3L.

H

WB

DOT1L antibody (pAb)

Raised against a peptide to the C-terminal region of human DOTL1.

HWBNEW DNMT3B antibody (mAb) (Clone 2C1)

Immunogen

H

WB

DR4 antibody (mAb) (Clone 32A1380)
DR6 antibody (pAb)
DRAK1 antibody (pAb)
DRAK2 antibody (pAb)

Raised against a recombinant protein containing amino acids 301-410 ofhuman DP1.
Raised against amino acids 1-20 of human DR4.
Raised against amino acid residues 371-655 of human DR6.
Raised against amino acids 5 to 9 of human DRAK1.
Raised against amino acids 351-365 of human DRAK2.

H
H
H, M
H

FC, WBWB
WB
WB

Drosha antibody (pAb)

Raised against a peptide containing the C-terminal region of human Drosha.

H

WB

E1A antibody (mAb) (Clone M73)

Raised against full-length recombinant Adenovirus 2 E1A.

Cells infected with
Adenovirus

IF, IP, WB

EDF1 antibody (pAb)

Raised against a peptide to the N-terminal region of human EDF1.

HWB

EED antibody (mAb) (Clone 41D)

Raised against full-length recombinant human EED protein.

H

EGF Receptor phospho Tyr992 antibody (pAb)

Raised against a peptide with phospho-tyrosine 992 of human EGFR.
Raised against a against a full-length recombinant protein to mouse EKLF
(Kruppel-like factor 1 (erythroid)).

H

ChIP, ChCh, ChSeq,
IHC, WB
WB

M

ChIP, IP, WB

DP1 antibody (pAb)

EKLF antibody (mAb) (Clone 7B2)
Elk-1 antibody (pAb)

Raised against a peptide within the C-terminal region of human Elk-1.

H

WB

ELP3 antibody (pAb)

Raised against a peptide to the N-terminal region of human ELP3.

H

WB

Estrogen Receptor a antibody (mAb) (Clone F3)

Raised against a peptide containing the C-terminal region of human ERa.

H

IP, WB

Estrogen Receptor b antibody (mAb)(Clone CO1531)

Raised against amino acids 212-477 of human ERb.

H

ICC, IF, IP, WB

ERR-a antibody (pAb)

Raised against a peptide containing the C-terminal region of human ERR-a.

H

WB

ETO / RUNX1T1 antibody (pAb)

Raised against a peptide within the internal region of human ETO / RUNX1T1.

H

WB

Ets-1 antibody (pAb)

Raised against the C-terminal half of recombinant mouse Ets-1.

H

WB

FormatCatalog No.

100 µg10 µg100 µl10 µl100 µl10 µl100 µl10 µl100 µg100 µg100 µg100 µg100 µl10 µl100 µg10 µg100 µl10 µl100 µg10 µg200 µl100 µg10 µg100 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg100 µl10 µl100 µl10 µl200 µl
10 µl

61503
61504
39907
39908
39953
39954
61145
61146
40934
40927
40923
40926
39783
39784
39771
39772
61257
61258
61203
61204
39094
61233
61234
61301
61302
39949
39950
61035
61036
39767
39768
61047
61048
61109
61110
39580
39581

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

7

Antibody

Immunogen

EuHMT1 antibody (pAb)

Raised against a peptide to the N-terminal region of human EuHMT1.

Reactivity

Applications

H

WB

Raised against a recombinant protein containing amino acids 353-451 of human
EZH2.
Raised against a recombinant protein containing amino acids 1-370 of mouse
EZH2.
Raised against a recombinant protein containing amino acids 1-370 of mouse
EZH2.

H, M

ChIP, IF, IP

H, M

ChIP, ChCh,
ChSeq, WB

H, M

WB

EZH2 phospho Thr345 antibody (pAb)

Raised against a peptide with phospho-threonine 345 of mouse EZH2.

H, M

DB, WBFancD2 antibody (pAb)

Raised against a peptide within the internal region of human FancD2.

H

WB

c-Fos antibody (pAb)

Raised against a peptide to human c-Fos.

H

WB

FosB antibody (mAb) (Clone 83B1138)

Raised against amino acids 2-14 of human FosB.

H

WB

FOXG1 antibody (pAb)

Raised against a peptide to the C-terminal region of human FOXG1.

HWB

FOXL2 antibody (pAb)

Raised against a peptide to the N-terminal region of human FOXG1.

HWB

FOXO1/FKHR antibody (pAb)

Raised against a peptide to the C-terminal region of human FOXO1/FKHR.

H

WB

FOXP1 antibody (mAb) (Clone 1G1)

Raised against a recombinant protein containing amino acids 495-578 of mouse
FOXP1. This antibody detects three isoforms.

M

ChIP, EMSA,
IP, WBFTO antibody (pAb)

Raised against a peptide to the N-terminal region of human FTO.

H

WB

GATA-1 antibody (pAb)

Raised against amino acids 66-78 of human GATA-1.

H, M, R

ChIP, WB

GATA-4 antibody (pAb)

Raised against a peptide to the C-terminal region of human GATA-4.

H

WB

GATA-6 antibody (pAb)

Raised against a peptide to the N-terminal region of human GATA-6.

H

WB

GCN5 antibody (pAb)

Raised against a peptide to the N-terminal region of human GCN5.

HWB

GCN5 antibody (mAb) (Clone 2GC-211)

Raised against a peptide to amino acids 18-132 of human GCN5.

HELISA, IF, WB

Geminin antibody (pAb)

Raised against full-length recombinant human Geminin protein.

HIP, WB

GLI1 antibody (pAb)

Raised against a peptide containing the C-terminal region of human GLI1.

H

WB

EZH2 antibody (mAb) (Clone AC22)
EZH2 antibody (pAb)
EZH2 antibody (pAb)

FormatCatalog No.

100 µg10 µg100 µg10 µg100 µl10 µl100 µl10 µl100 µl10 µl100 µl10 µl100 µl10 µl100 µg100 µl10 µl100 µl10 µl200 µl10 µl100 µg10 µg100 µl10 µl100 µl100 µl10 µl100 µl10 µl100 µl10 µl100 µg100 µl10 µl100 µl
10 µl

39777
39778
39875
39876
39901
39902
39933
39934
61241
61242
61057
61058
61421
61422
40960
61211
61212
61283
61284
39629
39630
61309
61310
61431
61432
39025
39893
39894
61063
61064
39831
39832
39975
61135
61136
61215
61216

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

8

Antibody
Goosecoid antibody (pAb)
HA Tag antibody (mAb) (Clone 3B9)
Hbo1 antibody (pAb)
HDAC1 antibody (mAb) (Clone 10E2)
HDAC1 antibody (mAb) (Clone 10E2)
HDAC1 antibody (pAb)

ImmunogenRaised against a peptide to the N-terminal region of human homeobox protein
Goosecoid.
Raised against synthetic peptide containing multiple repeats of the HA tag
epitope, YPYDVPDYA.
Raised against a peptide to amino acids 158-172 of human Hbo1.
Raised against a KLH-peptide to amino acids 467-482 of human and murine
HDAC1. (Concentrated culture supernatant)
Raised against a peptide to amino acids 467-482 of murine HDAC1.
(Purified mouse IgG)
Peptide mixture of amino acids 1-5, 433-448, 467-482 of human HDAC1.

Reactivity

Applications

H

WB

WR

IP

H, WR

WB

H, M, R

ChIP, IF, IHC, IP, WB

H, M, R

WB

H, M, R

ChIP, ChCh,
ChSeq, IP, WB

H, M, R

ChIP, IF, IHC, IP, WB

H, M, R

WB

H

ChIP, WBHDAC3 antibody (pAb)

Raised against a KLH-peptide to amino acids 541-556 of human HDAC2.
(Concentrated culture supernatant)
Raised against a peptide to amino acids 541-556 of human HDAC2.
(Purified mouse IgG)
Raised against amino acids 2-17 of human HDAC3.

HDAC3 antibody (mAb) (Clone 3G6)

Raised against amino acids 411-428 of human HDAC3.

H

IHC, IP, WB

HDAC4 antibody (pAb)
HDAC5 antibody (pAb)
HDAC6 antibody (pAb)

Raised against amino acids 194-209 of human HDAC4.
Raised against amino acids 572-587 of human HDAC5.
Raised against amino acids 1-16 of human HDAC6.

H
H, M
H, M

ChIP, WB
ChIP, WB
ChIP, WBHDAC9 antibody (pAb)

Raised against a peptide containing the C-terminal region of human HDAC9.

HWB

HDAC11 antibody (pAb)

Raised against amino acids 238-251 of human HDAC11.
Raised against a partial protein including amino acids 329-530 of human
HIF-1 alpha.

H, M

WB

B, H, M, R

IHC

H

WB

M

IF

H

IF, IHC, IP, WB

HDAC2 antibody (mAb) (Clone 3F3)
HDAC2 antibody (mAb) (Clone 3F3)

HIF-1 alpha antibody (mAb) (Clone ESEE122)
HIF-1 alpha antibody (pAb)
HIPK1 antibody (mAb)
HIPK2 antibody (mAb)

Raised against a peptide to the N-terminal region of human HIF-1 alpha.
Raised against a recombinant protein including amino acids 702-925 of mouse
HIPK1.
Raised against a a recombinant protein including amino acids 898-1051 of mouse
HIPK2.

HIRA antibody (mAb) (Clone WC15)

Raised against amino acids 421-729 of GST-tagged human HIRA.

H, M

IP

HIRA antibody (mAb) (Clone WC119.2H11)

Raised against amino acids 421-729 of GST-tagged human HIRA.

H, M

WB

FormatCatalog No.

100 µl10 µl200 µg10 µg200 µl10 µl200 µl10 µl100 µg
10 µg

61121
61122
39627
39628
39105
39106
39531
39532
39881
39882

100 µg40967

200 µl10 µl100 µg10 µg100 µg100 µg10 µg100 µg100 µg100 µg100 µl10 µl200 µl100 µg10 µg100 µl10 µl100 µg10 µg100 µg10 µg200 µg10 µg200 µg
10 µg

39533
39534
39883
39884
40968
39717
39718
40969
40970
40971
39793
39794
39208
61275
61276
39665
39666
39725
39726
39677
39678
39555
39556
39557
39558

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

9

Antibody

Immunogen

Reactivity

Applications

FormatCatalog No.

Histone H1 antibody (pAb)

Raised against recombinant Drosophila histone H1.

Dr

ChIP, IF, IP, WB

Histone H1.0 antibody (pAb)

Raised against a peptide to the N-terminal region of human histone H1.0.

H, M

WB

Histone H1 antibody (pAb)

Raised against purified calf thymus histone H1. (Purified rabbit IgG)

H, WR

WB

Histone H1 antibody (pAb)

Raised against purified calf thymus histone H1. (Rabbit serum)

H, WR

IF, WB

Histone H1.5S17ph antibody (pAb)

Raised against a peptide with phospho-serine 17 of human histone H1.5.

H, WR

DB, WB

Histone H2A antibody (pAb)

Raised against recombinant yeast histone H2A. (Rabbit serum)

BY, FY

ChIP, WB

Histone H2A antibody (pAb)

Raised against recombinant yeast histone H2A. (Purified rabbit IgG)

BY, FYWB

Histone H2A antibody (pAb)

Peptide mixture of amino acids 1-5 and 81-96 of human histone H2A.

H

WB

Histone H2A, acidic patch antibody (pAb)

Raised against a peptide with amino acids 89-91 of human histone H2A.

H, WR

ChIP, WB

Histone H2A, C-terminal antibody (pAb)

Raised against a peptide with the C-terminal region of human histone H2A.

H, WR

WB

Histone H2AK5ac antibody (pAb)

Raised against a peptide with acetyl-lysine 5 of human histone H2A.

H, WR

DB, WB

Histone H2AK9ac antibody (pAb)

Raised against a peptide with acetyl-lysine 9 of human histone H2A.

H, WR

DB, WB

H, WR

DB, WB

H, WR

DB, WB

100 µl100 µl10 µl100 µg10 µg100 µl10 µl100 µl10 µl200 µl10 µl100 µg10 µg100 µg200 µl10 µl200 µl10 µl200 µl10 µl200 µl10 µl200 µl10 µl100 µg10 µg200 µl10 µl200 µl10 µl100 µg10 µg100 µl10 µl100 µg10 µg200 µl
10 µl

39575
61417
61418
61201
61202
39707
39708
61107
61108
39235
39236
39945
39946
39209
39111
39112
39591
39592
39107
39108
39109
39110
39391
39392
61195
61196
39271
39272
39593
39594
39871
39872
61427
61428
39873
39874
39115
39116

Histone H2AT120ph antibody (pAb)
Histone H2AT120ph antibody (pAb)

Raised against a peptide with phospho-threonine 120 of human histone H2A.
(Rabbit serum)
Raised against a peptide with phospho-threonine 120 of human histone H2A.
(Purified rabbit IgG)

Histone H2AS129ph antibody (pAb)

Raised against peptide with phospho-serine 129 of budding yeast H2A.

BY

ChIP, DB, IF, IP, WB

Histone macroH2A1 antibody (pAb)

Raised against the N-terminal half of recombinant mouse macroH2A1.

H, M

IF, WB

Histone macroH2A1.1 antibody (pAb)

Raised against a peptide to amino acids 198-226 of human macroH2A1.1.

H

IF, IHC, WB

Histone macroH2A1.2 antibody (mAb)

Raised against a peptide to amino acids 198-228 of human macroH2A1.2.

H

IF, IHC, WB

Histone macroH2A2 antibody (pAb)

Raised against a peptide to amino acids 162-372 of human macroH2A2.

HIF, IHC, WB

Histone H2A/H4S1ph antibody (pAb)

Raised against a peptide with phospho-serine 1 of human histone H2A.

H, WR

DB, WB

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

10

Antibody

Immunogen

Reactivity

Applications

FormatCatalog No.

Histone H2Av antibody (pAb)

Raised against a peptide in the C-terminus of the Drosophila melanogasterhistone variant H2Av.

Dr

ChIP, ChCh,
ChSeq, WB

Histone H2A.X antibody (pAb) (H2AX)

Raised against a peptide to the C-terminus of human histone H2AX.

H

IF, WB

Histone H2A.XS139ph antibody (pAb)

Raised against a peptide with phospho-serine 139 of human histone H2AX.

H, WR

DB, IF, WB

H, WR

ChIP, DB, WB

H, WR

ChIP, ChCh, ChSeq,
DB, IP, WB

BY

ChIP, WB

FY

WB

FY

DB, WB

100 µl10 µl100 µl10 µl200 µg10 µg100 µg10 µg200 µl10 µl100 µl10 µl100 µl10 µl100 µl10 µl200 µl10 µl200 µl10 µl100 µg10 µg100 µg100 µg10 µg200 µl10 µl100 µl10 µl100 µl
10 µl

39715
39716
39689
39690
39117
39118
39943
39944
39113
39114
39647
39648
39640
39641
39642
39643
39125
39126
39237
39238
39947
39948
39210
61037
61038
39123
39124
61439
61440
39669
39670

100 µg61011

100 µl10 µl200 µl
10 µl

61321
61322
39121
39122

Pht1 / Histone H2A.Zac (pan-acetyl) antibody(pAb)

Raised against a peptide to the C-terminus of human histone H2A.Z.
(Purified rabbit IgG)
Raised against a peptide to the C-terminus of human histone H2A.Z.
(Rabbit serum)
Raised against full-length Htz1 protein, the S. cerevisiae homolog of Histone
H2A.Z.
Raised against a peptide with the C-terminus of Pht1, the Histone H2A variant
in S. pombe.
Raised against a peptide with acetyl-lysine at positions 5, 7, 12 and 16 from theamino terminus of Pht1, the Histone H2A variant in S. pombe.

Histone H2B antibody (pAb)

Raised against a peptide with the C-terminus of human histone H2B.

H, WR

WB

Histone H2B antibody (pAb)

Raised against recombinant yeast histone H2B. (Rabbit serum)

BY, FY

ChIP, WB

Histone H2B antibody (pAb)

Raised against recombinant yeast histone H2B. (Purified rabbit IgG)

BY, FYWB

Histone H2B antibody (pAb)

Raised against amino acids 111-125 of human histone H2B.

H

WB

Histone H2B antibody (mAb) (Clone 5HH2-2A8)

Raised against a peptide with the C-terminus of human histone H2B.

H

WB

Histone H2BK5ac antibody (pAb)

Raised against a peptide with acetyl-lysine 5 of human histone H2B.

H, WR

ChIP, ChCh, ChSeq,
DB, WB

Histone H2BK5me1 antibody (pAb)

Raised against a peptide with monomethyl-lysine 5 of human histone H2B.

H, WR

DB, WB

Histone H2BK12ac antibody (pAb)

Raised against a peptide with acetyl-lysine 12 of human histone H2B.

H, WR

ChIP, DB, WB

Histone H2BS14ph antibody (mAb)(Clone MABI 0251)

Raised against a peptide with phospho-serine 14 of human histone H2B.

H, WR

IF, IP, WB

Histone H2BK15ac antibody (pAb)

Raised against a peptide with acetyl-lysine 15 of human histone H2B.

H, WR

DB, WB

Histone H2BK16ac antibody (pAb)

Raised against a peptide with acetyl-lysine 16 of human histone H2B.

H, WR

ChIP, DB, WB

Histone H2A.Z antibody (pAb) (H2AZ)
Histone H2A.Z antibody (pAb) (H2AZ)
Htz1 / Histone H2A.Z antibody (pAb)
Pht1 / Histone H2A.Z antibody (pAb)

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

11

Antibody

Immunogen

Reactivity

Applications

Histone H2BK46ac antibody (pAb)

Raised against a peptide with acetyl-lysine 46 of human histone H2B.

H, WR

ChIP, DB, WB

Histone H2BK46me2 antibody (pAb)

Peptide containing dimethyl-lysine 46 of human histone H2B.

H, WR

DB, IF, WB

Histone H2BK120ac antibody (pAb)

Raised against a peptide with acetyl-lysine 120 of human histone H2B.

H, WR

ChIP, ChCh, ChSeq,
DB, WB

Histone H2BK120ub1 antibody (mAb) (Clone 56)

Raised against a branched peptide corresponding the the ubiquitin conjugationsite at Lys120 of human Histone H2B.

H, WR

ChIP, WB

Histone H3 antibody (mAb) (Clone MABI 0301)

Raised against an N-terminal peptide of human histone H3.

H, WR

ChIP, IF,TR-FRET, WB

Raised against a C-terminal peptide of human histone H3.

H, WR

ChIP, WB

Histone H3, C-terminal antibody (pAb)

Raised against a C-terminal peptide of human histone H3. (Rabbit serum)

BY, H, WR

ChIP, ELISA, WB

Histone H3, C-terminal antibody (pAb)

Raised against a C-terminal peptide of human histone H3. (Purified rabbit IgG)

H, WR

ChIP, IP, WB

Histone H3.cs1 antibody (pAb)

Raised against peptide with a Cathepsin L cleavage site at N-terminal histone H3.

H

WB

Histone H3ac (pan-acetyl) antibody (pAb)

Raised against peptide with acetyl-lysines at the H3 N-terminal tail.

H, WR

ChIP, DB, WB

Histone H3R2me2s (symmetric) antibody (pAb)

Raised against a peptide with dimethyl-arginine 2 (symmetric) of humanhistone H3.

H, WR

DB, WB

Histone H3T3ph antibody (pAb)

Raised against a peptide with phospho-threonine 3 of human histone H3.

H, WR

DB, WB

Histone H3K4ac antibody (pAb)

Raised against a peptide with acetyl-lysine 4 of human histone H3.

H, WR

ChIP, DB, IF, WB

Histone H3K4me1 antibody (mAb)(Clone MABI 0302)

Raised against a peptide with monomethyl-lysine 4 of human histone H3.

H, WRHistone H3K4me1 antibody (pAb)

Raised against peptide with monomethyl-lysine 4 of human histone H3.

H, M, WR

Histone H3K4me2 antibody (mAb)(Clone MABI 0303)

Raised against a peptide with dimethyl-lysine 4 of human histone H3.

H, M, WR

NEW Histone H3 antibody (mAb) (Clone 1B1-B2)

Histone H3K4me2 antibody (pAb)
Histone H3K4me2 antibody (pAb)
Histone H3K4me3 antibody (mAb)(Clone MABI 0304)

Raised against a peptide with dimethyl-lysine 4 of human histone H3.
(Rabbit serum)
Raised against a peptide with dimethyl-lysine 4 of human histone H3.
(Purified rabbit IgG)
Raised against a peptide with trimethyl-lysine 4 of human histone H3.

H, M, WR

ChIP, DB,
TR-FRET, WB
ChIP, ChCh, ChSeq,
DB, ELISA, IF, IP, WB
ChIP, DB, IF,
TR-FRET, WB
ChIP, ChCh, ChSeq,
DB, ELISA, IP, WB

H, M, WR

ChIP, DB, IF, WB

H, WR

IF, IP, WB

FormatCatalog No.

200 µl10 µl200 µl10 µl200 µl10 µl100 µg
10 µg

39571
39572
39567
39568
39119
39120
39623
39624

100 µg39763

100 µg10 µg200 µl10 µl100 µg10 µg200 µl10 µl200 µl10 µl100 µl10 µl200 µl10 µl200 µl
10 µl

61475
61476
39163
39164
61277
61278
39573
39574
39139
39140
39703
39704
39153
39154
39381
39382

100 µg39635

200 µl
10 µl

39297
39298

100 µg39679

200 µl10 µl100 µg
10 µg

39141
39142
39913
39914

100 µg61379

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

12

Antibody

Immunogen

Reactivity

FormatCatalog No.

ChIP, ChCh, ChSeq,
DB, ELISA, IF, IP, WB
ChIP, ChCh, ChSeq,
DB, IF, IP, WB

39159
39160
39915
39916
61391
61392
39673
39674
39651
39652
61251
61252
39917
39918
39137
39138
39585
39586
39241
39242
61399
61400
39887
39888

Histone H3K9ac antibody (pAb)

Raised against a peptide with acetyl-lysine 9 of human histone H3.

H, WR

DB, ELISA, IF, WB

Histone H3K9me1me2me3 antibody (pAb)

Raised against a peptide with methyl-lysine 9 of human histone H3.

H, WR

DB, IF, WB

Histone H3K9me0 antibody (mAb)(Clone 9B1-2G6)

Raised against a branched peptide corresponding to amino acids surrounding
Lys9 of human Histone H3.
Raised against a peptide with monomethyl-lysine 9 of human histone H3.
(Purified rabbit IgG)

H, WR

DB, WB

H, WR

ChIP, ChCh, ChSeq,
DB, WB

100 µl10 µl100 µg10 µg100 µl10 µl100 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg200 µl10 µl200 µl10 µl200 µl10 µl100 µg10 µg100 µg
10 µg

Histone H3K4me3 antibody (pAb)
Histone H3K4me3 antibody (pAb)

Raised against a peptide with trimethyl-lysine 4 of human histone H3.
(Rabbit serum)
Raised against a peptide with trimethyl-lysine 4 of human histone H3.
(Purified rabbit IgG)

Applications

Histone H3K4K9K18 (pan-biotinylated) antibody(pAb)

Raised against peptide with biotinylated-lysine 4 of human histone H3.

Histone H3R8me1 antibody (pAb)

BY, H, M, WR
BY, H, M, WR
H

DB, IF, WB

Raised against a peptide with monomethyl-arginine 8 of human histone H3.

H, WR

DB, WB

Histone H3R8me2a (asymmetric) antibody (pAb)

Raised against a peptide with dimethyl-arginine 8 (asymmetric) of humanhistone H3.

H

DB, WB

Histone H3K9ac antibody (mAb) (Clone 1B10)

Raised against a peptide with acetyl-lysine 9 of human histone H3.

Histone H3K9ac antibody (pAb)
Histone H3K9ac antibody (pAb)

Histone H3K9me1 antibody (pAb)

Raised against a peptide with acetyl-lysine 9 of human histone H3.
(Purified rabbit IgG)
Raised against a peptide with acetyl-lysine 9 of human histone H3.
(Rabbit serum)

H, M, WR
H, M, WR
H, M, WR

ChIP, ChCh, ChSeq,
DB, IF, IP, WB
ChIP, ChCh, ChSeq,
DB, IF, IP, WB
ChIP, ChCh, ChSeq,
DB, WB

Histone H3K9me1 antibody (mAb)(Clone MABI 0306)

Raised against a peptide with monomethyl-lysine 9 of human histone H3.

H, WR

ChIP, DB, WB

100 µg39681

Histone H3K9me1 antibody (pAb)

Raised against a peptide with monomethyl-lysine 9 of human histone H3.
(Rabbit serum)

H, WR

DB, IF, WB

200 µl
10 µl

39249
39250

Histone H3K9me2 antibody (mAb)(Clone MABI 0307)

Raised against a peptide with dimethyl-lysine 9 of human histone H3.

H, WR

ChIP, DB, IF,
TR-FRET, WB

100 µg39683

Histone H3K9me2 antibody (pAb)

Raised against a peptide with dimethyl-lysine 9 of human histone H3.

H, M, WR

ChIP, DB, IF, IP, WB

H, WR

ChIP, DB, ELISA, IF,
WB

H, WR

ChIP, DB, IF, WB

200 µl10 µl200 µl10 µl100 µg
10 µg

39239
39240
39375
39376
39753
39754

H, WR

ChIP, DB, IF, IP, WB

100 µg61013

Histone H3K9me2 antibody (pAb)
Histone H3K9me2 antibody (pAb)
Histone H3K9me3 antibody (mAb)(Clone MABI 0319)

Raised against a peptide with dimethyl-lysine 9 of human histone H3.
(Rabbit serum)
Raised against a peptide with dimethyl-lysine 9 of human histone H3.
(Purified rabbit IgG)
Raised against peptide with trimethyl-lysine 9 of human histone H3.

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

13

Antibody

Immunogen

Reactivity

Applications

Histone H3K9me3 antibody (mAb)(Clone 2AG-6F12-H4)

Raised against a peptide with trimethyl-lysine 9 of human histone H3.

H, WR

DB, IF, IP, WB

FY, H, WR

ChIP, ChCh, ChSeq,
DB, ELISA, IF, IP, WB

H, WR

ChIP, DB, IF, WB

H, WR

DB, IF, WB

Raised against a peptide with phospho-serine 10 of human histone H3.

H, WR

ELISA, IF

Histone H3S10ph antibody (pAb)

Raised against a peptide with phospho-serine 10 of human histone H3.

H, WR

ChIP, DB, IF, WB

Histone H3S10phS28ph antibody (pAb)

Raised against a peptide with phospho-serine 28 of human histone H3.

H, WR

ChIP, DB, IF, WB

Histone H3T11ph antibody (mAb) (Clone N123/48)

Raised against a peptide with phospho-threonine 11 of human histone H3.

H

DB, ICC, WB

H, WR

ChIP, ChCh, ChSeq,
DB, WB

H, WR

DB, WB

H, M, WR

ChIP, ChCh, ChSeq,
DB, ELISA, IP, WB

H, M, WR

ChIP, DB, WB

Raised against a peptide with acetyl-lysine 14 of human histone H3.

H, M, WR

ChIP

Raised against a peptide with monomethyl-lysine 14 of human histone H3.

H, WR

WB

Raised against a peptide with dimethyl-lysine 14 of human histone H3.

H, WR

DB, WB

H, WR

DB, WB

H, WR

ChIP, ChCh, ChSeq,
DB, IF, IP, WB

H, WR

ChIP, DB, IF, WB

H, WR

ChIP, DB, IF, WB

Histone H3K9me3 antibody (pAb)
Histone H3K9me3 antibody (pAb)
Histone H3K9me2S10ph / H3K27me2S28ph
antibody (mAb) (Clone 51Ta2H12)
Histone H3S10ph antibody (mAb)(Clone MABI 0312)

Histone H3T11ph antibody (pAb)
Histone H3T11ph antibody (pAb)
Histone H3K14ac antibody (pAb)
Histone H3K14ac antibody (pAb)
Histone H3K14ac antibody (mAb)(Clone 13HH3-1A5)
NEW Histone H3K14me1 antibody (pAb)

Histone H3K14me2 antibody (pAb)
Histone H3R17me2a (asymmetric) antibody (pAb)
Histone H3K18ac antibody (pAb)
Histone H3K18ac antibody (pAb)
Histone H3K18ac antibody (pAb)

Raised against a peptide with trimethyl-lysine 9 of human histone H3.
(Rabbit serum)
Raised against a peptide with trimethyl-lysine 9 of human histone H3.
(Purified rabbit IgG)
Raised against a peptide with amino acid residues 180-195 of human TAF10.
It recognizes H3 doubly modified at K9me2S10ph and/or K27me2S28ph.

Raised against a peptide with phospho-threonine 11 of human histone H3.
(Rabbit serum)
Raised against a peptide with phospho-threonine 11 of human histone H3.
(Purified rabbit IgG)
Raised against a peptide with acetyl-lysine 14 of human histone H3.
(Rabbit serum)
Raised against a peptide with acetyl-lysine 14 of human histone H3.
(Purified rabbit IgG)

Raised against a peptide with dimethyl-arginine 17 (asymmetric) of humanhistone H3.
Raised against a peptide with acetyl-lysine 18 of human histone H3.
(Purified rabbit IgG)
Raised against a peptide with acetyl-lysine 18 of human histone H3.
(Rabbit serum)
Raised against a peptide with acetyl-lysine 18 of human histone H3.

FormatCatalog No.

200 µl10 µl200 µl10 µl100 µg10 µg100 µg
10 µg

39285
39286
39161
39162
39765
39766
61429
61430

100 µg39636

200 µl10 µl200 µl10 µl100 µg10 µg200 µl10 µl100 µg10 µg200 µl10 µl100 µg
10 µg

39253
39254
39147
39148
39821
39822
39151
39152
61197
61198
39599
39616
39697
39698

100 µg61433

100 µl10 µl200 µl10 µl100 µl10 µl100 µg10 µg200 µl10 µl100 µl
10 µl

61509
61510
39349
39350
39709
39710
39755
39756
39587
39588
39693
39694

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

14

Antibody

Immunogen

Reactivity

Applications

Histone H3K18ac antibody (pAb)

Raised against a peptide with acetyl-lysine 18 of human histone H3.

Histone H3K18me1 antibody (pAb)

Raised against a peptide with monomethyl-lysine 18 of human histone H3.

Histone H3K23ac antibody (pAb)

H, WR

DB, IF, WB

HDB, WB

Raised against a peptide with acetyl-lysine 23 of human histone H3.

H, WR

ChIP, DB, IF, WB

Histone H3K23me1 antibody (pAb)

Raised against a peptide with monomethyl-lysine 23 of human histone H3.

H, WR

DB, WB

Histone H3K23me2 antibody (pAb)

Raised against a peptide with dimethyl-lysine 23 of human histone H3.

H

DB, WBNEW Histone H3K23me3 antibody (pAb)

Raised against a peptide with trimethyl-lysine 23 of human histone H3.

H, WR

DB

Histone H3K23pr antibody (pAb)

Raised against a peptide with propionyl-lysine 23 of human histone H3.

H

DB, WB

Histone H3K27ac antibody (mAb)(Clone MABI 0309)

Raised against a peptide with acetyl-lysine 27 of human histone H3.

H, WRHistone H3K27ac antibody (pAb)
Histone H3K27ac antibody (pAb)
Histone H3K27me1 antibody (mAb)(Clone MABI 0321)
Histone H3K27me1 antibody (pAb)
Histone H3K27me1 antibody (pAb)
Histone H3K27me2 antibody (pAb)
Histone H3K27me2 antibody (pAb)
Histone H3K27me2 antibody (mAb)(Clone MABI 0324)
Histone H3K27me2me3 antibody (mAb)(Clone 7B11)
Histone H3K27me2me3 antibody (mAb)(Clone 7B11)
Histone H3K27me3 antibody (mAb)(Clone MABI 0323)

Raised against a peptide with acetyl-lysine 27 of human histone H3.
(Purified rabbit IgG)
Raised against a peptide with acetyl-lysine 27 of human histone H3.
(Rabbit serum)
Raised against peptide with monomethyl-lysine 27 of human histone H3.
Raised against peptide with monomethyl-lysine 27 of human histone H3.
(Rabbit serum)
Raised against peptide with monomethyl-lysine 27 of human histone H3.
(Purified rabbit IgG)
Raised against a peptide with dimethyl-lysine 27 of human histone H3.
(Rabbit serum)
Raised against a peptide with dimethyl-lysine 27 of human histone H3.
(Purified mouse IgG)
Raised against a peptide with dimethyl-lysine 27 of human histone H3.
Raised against a peptide with trimethyl-lysine 27 of human histone H3.
(Ascites)Raised against a peptide with trimethyl-lysine 27 of human histone H3.
Raised against peptide with trimethyl-lysine 27 of human histone H3.

BY, H, WR
BY, H, WR
H, WR

FormatCatalog No.

200 µl10 µl100 µl10 µl200 µl10 µl200 µl10 µl100 µl10 µl100 µl10 µl100 µl
10 µl

39129
39130
39667
39668
39131
39132
39387
39388
39653
39654
61499
61500
61397
61398

ChIP, DB, IF, WB

100 µg

39685

ChIP, ChCh, ChSeq,
DB, IF, IP, WB
ChIP, ChCh, ChSeq,
DB, WB
ChIP, DB, IF, IP,
TR-FRET, WB

100 µg10 µg200 µl
10 µl

39133
39134
39135
39136

100 µg61015

200 µl10 µl100 µg10 µg200 µl10 µl100 µg
10 µg

39377
39378
39889
39890
39245
39246
39919
39920

H, WR

DB, IF, WB

H, WR

DB, WB

H, WR

ChIP, DB, IF, WB

H, WR

DB, IF, WB

H, WR

ChIP, IF, IP, WB

100 µg61435

H, M, WR

ChIP, ChCh, ChSeq,
WB

H, WR

IF, WB

200 µl10 µl200 µl
10 µl

39535
39537
39536
39538

H, M, WR

ChIP, DB, IF, IP,
TR-FRET, WB

100 µg

61017

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

15

Antibody

Immunogen

Reactivity

Applications

FormatCatalog No.

H, M, WR

ChIP, ChCh, ChSeq,
DB, IF, IP, WB

H, M, WR

ChIP, DB, ELISA, WB

200 µg10 µg200 µl
10 µl

39155
39157
39156
39158

H, M, RELISA, IF, WB

100 µl39098

H, M, RELISA, WB

100 µl200 µl10 µl200 µl10 µl200 µl10 µl100 µl
10 µl

61325
39149
39150
39637
39638
39379
39380
61351
61352

100 µg61019

200 µl10 µl100 µg
10 µg

39255
39256
39891
39892

Histone H3S28ph antibody (mAb) (Clone HTA28)

Raised against a peptide with trimethyl-lysine 27 of human histone H3.
(Purified rabbit IgG)
Raised against a peptide with trimethyl-lysine 27 of human histone H3.
(Rabbit serum)
Raised against a peptide with phospho-serine 28 of human histone H3.
(Purified rat IgG)
Raised against a peptide with phospho-serine 28 of human histone H3. (Ascites)

Histone H3S28ph antibody (pAb)

Raised against a peptide with phospho-serine 28 of human histone H3.

H, WR

DB, WB

Histone H3.3S31ph antibody (pAb)

Raised against a peptide with phospho-serine 31 of human histone H3.3.

H

DB, WB

Histone H3K36ac antibody (pAb)

Raised against a peptide with acetyl-lysine 36 of human histone H3.

H, WR

ChIP, ChCh, ChSeq,
DB, IF, WB

Histone H3K36me1 antibody (pAb)

Raised against a peptide with monomethyl-lysine 36 of human histone H3.

H, WR

DB, WB

Histone H3K36me2 antibody (mAb)(Clone MABI 0332)

Raised against a peptide with dimethyl-lysine 36 of human histone H3.

H, WRHistone H3K27me3 antibody (pAb)
Histone H3K27me3 antibody (pAb)
Histone H3S28ph antibody (mAb) (Clone HTA28)

Histone H3K36me2 antibody (pAb)
Histone H3K36me2 antibody (pAb)

Raised against a peptide with dimethyl-lysine 36 of human histone H3.
(Rabbit serum)
Raised against a peptide with dimethyl-lysine 36 of human histone H3.
(Purified rabbit IgG)

BY, H, WR

ChIP, DB, IF, IP,
TR-FRET, WB
ChIP, ChCh, ChSeq,
DB, IF, IP, WB

BY, H, WR

DB, WB

Histone H3K36me3 antibody (mAb)(Clone MABI 0333)

Raised against peptide with trimethyl-lysine 36 of human histone H3.

H, M, WR

ChIP, DB, IF, IP, WB

100 µg61021

Histone H3K36me3 antibody (pAb)

Raised against peptide with trimethyl-lysine 36 of human histone H3.

H, M, WR

ChIP, ChCh, ChSeq,
DB, IP, WB

Histone H3T45ph antibody (pAb)

Raised against a peptide with phospho-threonine 45 of human histone H3.

BY, H, WR

DB, WB

Histone H3K56ac antibody (mAb) (Clone 12.1)

Raised against peptide with acetyl-lysine 56 of yeast histone H3.

BY, FY, H

ChIP, DB

Histone H3K56ac antibody (pAb)

Raised against peptide with acetyl-lysine 56 of yeast histone H3.

BY, H

ChIP, ChCh, ChSeq,
DB, WB

Histone H3K56me1 antibody (pAb)

Raised against peptide with monomethyl-lysine 56 of human histone H3.

H

DB, WB

Histone H3K56me2 antibody (pAb)

Raised against a peptide with dimethyl-lysine 56 of human histone H3.

H

DB, WB

Histone H3K79ac antibody (pAb)

Peptide containing acetyl-lysine 79 of human histone H3.

H, WR

ChIP, DB, WB

100 µl10 µl100 µl10 µl100 µg10 µg200 µl10 µl200 µl10 µl200 µl10 µl200 µl
10 µl

61101
61102
39737
39738
61061
61062
39281
39282
39273
39274
39277
39278
39565
39566

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

16

Antibody
Histone H3K79me1 antibody (pAb)
Histone H3K79me1 antibody (pAb)
Histone H3K79me2 antibody (pAb)
Histone H3K79me2 antibody (pAb)

ImmunogenRaised against a peptide with monomethyl-lysine 79 of human histone H3.
(Rabbit serum)
Raised against a peptide with monomethyl-lysine 79 of human histone H3.
(Purified rabbit IgG)
Raised against a peptide with dimethyl-lysine 79 of human histone H3.
(Rabbit serum)
Raised against a peptide with dimethyl-lysine 79 of human histone H3.
(Purified rabbit IgG)

Histone H3K122me1 antibody (pAb)

Raised against a peptide with monomethyl-lysine 122 of human H3.

Histone H3.X/Y antibody (mAb) (Clone 8H6-2111)

Raised against a peptide containing amino acids 9-20 of human histone H3.X.

Histone H4 antibody (pAb)
Histone H4 antibody (pAb)

Raised against full-length recombinant Xenopus histone H4.
(Rabbit serum)
Raised against full-length recombinant Xenopus histone H4.
(Purified rabbit IgG)

Histone H4 antibody (pAb)

Raised against a peptide containing amino acids 43-54 of human histone H4.

Histone H4ac (pan-acetyl) antibody (mAb)(Clone 3HH4-3H10)

Raised against a peptide containing amino acids 1- 24 of human histone H4acetylated at lysines 5, 8, 12 and 16.
Raised against a peptide containing amino acids 1- 24 of human histone H4acetylated at lysines 5, 8, 12 and 16.
Raised against a peptide of Tetrahymena histone H2A acetylated at multiple
lysines. (Purified rabbit IgG)
Raised against a peptide of Tetrahymena histone H2A acetylated at multiple
lysines. (Rabbit serum)

Histone H4ac (pan-acetyl) antibody (mAb)
Histone H4ac (pan-acetyl) antibody (pAb)
Histone H4ac (pan-acetyl) antibody (pAb)
Histone H4ac (pan-acetyl) antibody (pAb)
Histone H4R3me2a (asymmetric) antibody (pAb)
Histone H4R3me2s (symmetric) antibody (pAb)
Histone H4K5ac antibody (pAb)
Histone H4K5ac antibody (pAb)
Histone H4K5ac antibody (pAb)

Raised against a peptide of Tetrahymena hv1 acetylated at multiple lysines.
Raised against a peptide with dimethyl-arginine 3 (asymmetric) of humanhistone H4.
Raised against a peptide with dimethyl-arginine 3 (symmetric) of humanhistone H4.
Raised against a peptide with acetyl-lysine 5 of human histone H4.
Raised against a peptide with acetyl-lysine 5 of human histone H4.
(Rabbit serum)
Raised against a peptide with acetyl-lysine 5 of human histone H4.
(Purified rabbit IgG)

Reactivity

Applications

H, WR

ChIP, DB, WB

H, WR

ChIP, DB, WB

H, WR

ChIP, ChCh, ChSeq,
DB, WB

H, WR

ChIP, DB, WB

H, WR

DB, WBH

IF, IHC, WB

H, WR

WB

H, WR

WB

H, WR

ChIP, IP, WB

H, M, WR

ChIP, WB

H, WR

ELISA, ICC, IF,
IP, WB

H, M, WR

ChIP, DB, IF, WB

H, M, WR

ChIP, DB, IF, WB

BY, H, WR

IF, WB

H, WR

DB, IF, WB

H, WR

DB, WB

H, M, WR

ChIP, DB, IF, WB

H, M, WR

ChIP, DB, IF, WB

H, M, WR

ChIP, DB, WB

FormatCatalog No.

200 µl10 µl100 µg10 µg200 µl10 µl100 µg10 µg200 µl10 µl100 µg10 µg200 µl10 µl100 µg10 µg100 µl10 µl100 µg
10 µg

39145
39146
39921
39922
39143
39144
39923
39924
39367
39368
61161
61162
39269
39270
61199
61200
61299
61300
61337
61338

100 µg39967

100 µg10 µg200 µl
10 µl
50 µg
10 µg
200 µl10 µl100 µl10 µl200 µl10 µl200 µl10 µl100 µg
10 µg

39925
39926
39243
39244
39177
39227
39705
39706
61187
61188
39169
39170
39583
39584
39699
39700

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

17

Antibody

Immunogen

Reactivity

Applications

Histone H4K8ac antibody (pAb)

Raised against a peptide with acetyl-lysine 8 of human histone H4.

H, M, WR

ChIP, DB, WB

Histone H4K8ac antibody (pAb)

Raised against a peptide with acetyl-lysine 8 of human histone H4.

H, WR

WB

Histone H4K8K12K16 (pan-biotinylated) antibody(pAb)

Raised against peptide with biotinylated-lysine 12 of human histone H4.

H

DB

BY, H, M, WR

ChIP, ChCh, ChSeq,
DB, IF, WB

BY, H, M, WR

ChIP, DB, IF, WB

Histone H4K12ac antibody (pAb)
Histone H4K12ac antibody (pAb)
Histone H4K16ac antibody (pAb)
Histone H4K16ac antibody (pAb)
Histone H4H18ph antibody (pAb)
Histone H4H18ph antibody (pAb)

Raised against a peptide with acetyl-lysine 12 of human histone H4.
(Rabbit serum)
Raised against a peptide with acetyl-lysine 12 of human histone H4.
(Purified rabbit IgG)
Raised against a peptide with acetyl-lysine 16 of human histone H4.
(Rabbit serum)
Raised against a peptide with acetyl-lysine 16 of human histone H4.
(Purified rabbit IgG)
Raised against a peptide containing a 3-phosphohistidine analog at Histidine 18
of human Histone H4. (Rabbit serum)
Raised against a peptide containing a 3-phosphohistidine analog at Histidine 18
of human Histone H4. (Purified rabbit IgG)

BY, Dr, H,
M, WR
BY, Dr, H,
M, WR

ChIP, DB, IP, WB
ChIP, DB, WB

H, WR

DB, WB

H, WR

DB, WB

Histone H4K20me1 antibody (mAb)(Clone 5E10-D8)

Raised against a peptide with monomethyl-lysine 20 of human histone H4.

H, WR

IF, WB

Histone H4K20me2 antibody (mAb)

Raised against a peptide with dimethyl-lysine 20 of human histone H4.

H, WR

WB

Histone H4K20me2 antibody (pAb)

Raised against a peptide with dimethyl-lysine 20 of human histone H4.

H, WR

ChIP, IF, WB

Histone H4K20me3 antibody (mAb)(Clone 6F8-D9)Raised against a peptide with trimethyl-lysine 20 of human histone H4.

H, M, WR

ChIP, DB, WB

Histone H4K31me1 antibody (pAb)

Raised against a peptide with monomethyl-lysine 31 of human histone H4.

H, WR

DB, WB

HMG-1 antibody (pAb)

Raised against amino acids 167-182 of human HMG-1.

H

WB

HMG-2 antibody (pAb)

Raised against amino acids 166-181 of human HMG-2.

H

WB

HMGA1 antibody (pAb)

Raised against a peptide containing the N-terminal region of human HMGA1.

H

IF, WB

HMGA2 / HMGI-C antibody (pAb)

Raised against a peptide containing the N-terminal region of human HMGA2

HWB

HMGN1 antibody (pAb)

Raised against a peptide containing the C-terminal region of human HMGN1.

H

WB

FormatCatalog No.

100 µl10 µl200 µl10 µl100 µl10 µl200 µl10 µl100 µg10 µg200 µl10 µl100 µg10 µg100 µl10 µl100 µg10 µg100 µg10 µg200 µl10 µl200 µl10 µl100 µg10 µg200 µl10 µl200 µl10 µl100 µl100 µl10 µl100 µl10 µl100 µl
10 µl

61103
61104
39171
39172
61393
61394
39165
39166
39927
39928
39167
39168
39929
39930
61411
61412
61413
61414
39727
39728
39539
39540
39173
39174
39671
39672
39385
39386
39551
39552
39029
39615
39650
61041
61042
39833
39834

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

18

Antibody

ImmunogenHmgn2 antibody (pAb)

Raised against a peptide within the N-terminal region of human Hmgn2.

Reactivity

Applications

H

WB

H, M

IHC, WBH

IHC, WB

FormatCatalog No.
61353
61354
39837
39838
39827
39828
61189
61190
39825
39826
39295
39296
39977
39978
39979
39980
39981
39982
39655
39656
61207
61208
61209
61210
39355
39356
39291
39292
40903

HNF4A antibody (pAb)

Raised against a peptide containing the N-terminal region of human HNF4A.

H

WB

HOXA9 antibody (pAb)

Raised against a peptide containing the N-terminal region of human HOXA9.

H

WB

HP1a antibody (pAb)

Raised against a recombinant protein to full-length mouse HP1a.

H, M

WB

HP1a antibody (mAb) (Clone 2HP-1H5)

Raised against a recombinant protein with amino acids 67-119 of mouse HP1a.

H, M

HP1b antibody (mAb) (Clone 1MOD-1A9)

Raised against a recombinant mouse HP1b.

H, M

HP1g antibody (mAb) (Clone 2MOD-1G6)

Raised against a recombinant full-length mouse HP1g.

H, M

HP1 gamma phospho Ser93 antibody (pAb)

Raised against a peptide with phospho-serine 93 of human HP1 gamma.

H

DB, WB

HSC70 / HSP7C antibody (mAb) (Clone 6E1)

Raised against full-length human HSC70 / HSP7C protein.

HIF, IP, WB

HSP72 antibody (mAb) (Clone 3G7)

Raised against full-length human HSP72 protein.

HIF, IP, WB

Ikaros antibody (mAb) (Clone 2A9)

Raised against the N-terminal half of mouse Ikaros.

M

WB

Ikaros antibody (pAb)

Raised against the N-terminal half of mouse Ikaros.

M

WB

IkBa antibody (mAb) (Clone 6A920)
IkBa phospho Ser32,36 antibody (mAb)(Clone 39A1431)
IKKa antibody (mAb) (Clone 14A231)
IKKb antibody (mAb) (Clone 10AG2)
IKKb antibody (mAb) (Clone 10A9B6)
IKKg antibody (mAb) (Clone 46B844)
IKKi/IKKe antibody (mAb) (Clone 72B587)
IKKi/IKKe antibody (pAb)

Raised against amino acids 32-391 of recombinant human IkBa.

H, M

IP, WB

100 µl10 µl100 µg10 µg100 µl10 µl100 µl10 µl100 µl10 µl200 µl10 µl100 µg10 µg100 µg10 µg100 µg10 µg100 µl10 µl100 µg10 µg100 µg10 µg200 µg10 µg200 µl10 µl100 µgRaised against a peptide with phospho-serine 32 and 36 of human IkBa.

H

WB

100 µg40904

Raised against full-length human IKKa.
Raised against 6xHis-tagged, full-length human IKKb.
Raised against 6xHis-tagged, full-length human IKKb.
Raised against full-length human IKKg.
Raised against amino acids 175-188 of human IKKi/IKKe.
Raised against amino acids 175-188, 525-540 & 567-580 of human IKKi/IKKe.

H, M
H
H
H
H, M, RH

IP, WB
WB
IP, WB
WB
WB
WB

100 µg100 µg100 µg100 µg100 µg100 µg

40905
40906
40907
40908
40950
40957

HNF-3a / FOXA1 antibody (mAb) (Clone 2F83)
HNF-3b / FOXA2 antibody (pAb)

Raised against a recombinant protein with amino acids 7-103 ofrat HNF-3a / FOXA1.
Raised against a recombinant protein with amino acids 7-86 ofrat HNF-3b / FOXA2.

ChIP, ELISA, ICC, IF,IHCELISA, IF, IHC,
IP, WBELISA, ICC, IF,
IHC, IP, WB

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

19

Antibody

Immunogen

Reactivity

Applications

INCENP antibody (mAb)

Raised against amino acids 231-398 of recombinant human INCENP.

H, M, WRIF

NEW ING1 antibody (mAb) (Clone Cab1)

Raised against a peptide with amino acids 67-279 of human ING1.

H

IF, WB

H, M, R
H
H
H

WB
WB
WB
WB

INHAT-1/TAF-1a/TAF-1b antibody (pAb)
INHAT-2/pp32 antibody (pAb)
IRAK-1 antibody (pAb)
IRAK-2 antibody (pAb)

Peptide mix of amino acids 66-8 &, 135-151 of human INHAT-1/TAF-1a/TAF-1b.
Peptide mix of amino acids 87-103 and 231-246 of human INHAT-2/pp32.
Raised against amino acids 700-712 of human IRAK-1.
Raised against amino acids 546-564 of human IRAK-2.

IRF-2 antibody (pAb)

Raised against a peptide within the C-terminal region of human IRF-2.

H

WB

IRF-3 antibody (pAb)

Raised against amino acids 306-312 of human IRF-3.

H

WB

IRF-3 antibody (pAb)

Raised against full-length human IRF-3.

B, H

ChIP, IF, WB

Raised against a peptide within the C-terminal region of human IRF-4.

H

WB

IRF-5 antibody (pAb)

Raised against a peptide containing the N-terminal region of human IRF-5.

H

WB

JARID1C antibody (pAb)

Raised against a peptide to the C-terminus of human JARID1C.

H, M

ChIP, IP, WB

Jhd2 antibody (pAb)

Raised against a peptide to the C-terminus of S. cerevisiae Jhd2.

BY

WB

JMJD2A antibody (mAb) (Clone N154/32)

Raised against a recombinant protein containing amino acids 586-674 of humanJMJD2A.

H, M

WB

JMJD2B / KDM4B antibody (pAb)

Raised against a peptide containing the C-terminal region of human JMJD2B.

H

WB

JMJD2D antibody (pAb)

Raised against amino acids 337-359 of human JMJD2D.

HWB

JMJD2F antibody (pAb)

Raised against a peptide to the C-terminus of human JMJD2F.

HWB

JMJD3 / KDM6B antibody (mAb) (Clone 67-A2)

Raised against a recombinant protein containing amino acids 1028-1684 ofhuman JMJD3 / KDM6B.

H, M

WB

Raised against a peptide containing the N-terminal region of human JMJD6.

H

WB

JunB antibody (pAb)

Raised against amino acids 28-42 of human JunB.

H

WB

c-Jun antibody (pAb)

Raised against amino acids 20-37 of human c-Jun.

H, M

ChIP, EMSA, IF

NEW IRF-4 antibody (pAb)

NEW JMJD6 antibody (pAb)

FormatCatalog No.

200 µg10 µg100 µg10 µg200 µl200 µl100 µg100 µg100 µl10 µl200 µl10 µl100 µl100 µl10 µl100 µl10 µl200 µl10 µl200 µl10 µl100 µg10 µg100 µl10 µl200 µl10 µl200 µl10 µl100 µg10 µg100 µl10 µl200 µl10 µl100 µg

39259
39260
61459
61460
39202
39213
40910
40912
61281
61282
39371
39372
39033
61511
61512
61269
61270
39229
39230
39263
39264
39815
39816
61221
61222
39247
39248
39257
39258
61387
61388
61493
61494
39549
39550
39309

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

20

Antibody

Immunogen

Reactivity

Applications

JunD antibody (pAb)

Raised against a peptide containing the N-terminal region of human JunD.

H

WB

Kaiso antibody (mAb) (Clone 6F1D)

Raised against amino acids 103-499 of His-tagged mouse Kaiso.

M

WB

Kat6a antibody (mAb) (Clone 2MOZ-2D4)

Raised against a peptide containing amino acids 856-869 of mouse Kat6a.

H, M

ChIP, IPNEW KDM1B / LSD2 antibody (pAb)

Raised against a peptide with the N-terminal region of human KDM1B / LSD2.

M

WB

Ki-67 antibody (pAb)

Raised against a peptide containing the N-terminal region of human Ki-67.

H

WB

KLF4 antibody (pAb)

Raised against recombinant full-length human KLF4.

H, WR

WB

H, M

WB

H

IHC, WB

M

WB

H

WB

H, WR

ChIP, IP, WB

H

ChIP, IP, WB

HIF, IP, WB

Hm, H, Mk, M, R

IF, IP, WB

HIF, IP, WB

KLF5 antibody (pAb)
KLF6 antibody (mAb) (Clone 2A2)
KLF6 antibody (pAb)
TIEG1 / KLF10 antibody (pAb)
L3MBTL1 antibody (pAb)
L3MBTL2 antibody (pAb)

Raised against a peptide derived from the N-terminus of mouse KLF5
(Krueppel-like factor 5).
Raised against a recombinant protein containing amino acids 28-199 of human
KLF6 (Krueppel-like factor 6).
Raised against a peptide derived from the internal region of human KLF6
(Krueppel-like factor 6).
Raised against a peptide derived from human TIEG1 / KLF10.
Raised against a His-tagged fusion protein containing amino acids 1-215 ofhuman L3MBTL2.
Raised against a His-tagged fusion protein containing amino acids 1-230 ofhuman L3MBTL2.

Lamin A, mature antibody (mAb) (Clone 4A4)Raised against a peptide within the C-terminal region of human Lamin A.

Lamin A/C antibody (mAb) (Clone 3A6-4C11)

Raised against amino acids 430-545 of recombinant human Lamin A.

Raised against a peptide with amino acids 449-457 of human Lamin A/C andcontaining the R453W substitution.
Raised against recombinant protein containing amino acids 430-545 of human
Lamin A/C R482W antibody (mAb) (Clone 5H8-B4)
Lamin A/C containing the R482W substitution.
Lamin B1 antibody (pAb)
Raised against full-length human Lamin B1.

H

IF, IP, WB

H, M

WB

LAP2 alpha antibody (mAb) (Clone 3A3)

Raised against a peptide with the C-terminus of human LAP2 alpha.

H

WB

LexA DNA-binding Domain antibody (pAb)

Raised against amino acids 1-222 of recombinant E. coli LexA.

NS

IP, WB

LIG1 antibody (pAb)

Raised against amino acids 51-68 of human LIG1.

HWB

Lamin A/C R453W antibody (mAb) (Clone 12A-2F5)

FormatCatalog No.

100 µl10 µl200 µg10 µg100 µg10 µg100 µl10 µl100 µl10 µl100 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg100 µl10 µl200 µl10 µl200 µl10 µl100 µg10 µg200 µg10 µg100 µg10 µg100 µg10 µg100 µl200 µl10 µl200 µl10 µl200 µl

61403
61404
39365
39366
61327
61328
61457
61458
39799
39800
39745
39746
61099
61100
61297
61298
61243
61244
61117
61118
39182
39183
39569
39570
39961
39962
39287
39288
39959
39960
39963
39964
39095
39267
39268
39184
39185
39214

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

21

Antibody

Immunogen

Reactivity

Applications

LIN28A antibody (pAb)

Raised against a peptide within the C-terminal region of human LIN28A.

H

WB

Livin/ML-IAP antibody (mAb) (Clone 88C570)

Raised against GST-tagged human Livin/ML-IAP.

H

LSD1 / KDM1A antibody (pAb)

Raised against full-length recombinant human LSD1 protein.

LXR-a antibody (pAb)

Raised against a peptide containing the N-terminal region of human LXR-a.

H, M

LXR-b antibody (pAb)

Raised against a peptide containing the N-terminal region of human LXR-b.

H

WB
ChIP, ChCh,
ChSeq, IP, WB
ChIP, ChCh,
ChSeq, IP, WB
ChIP, ChCh,
ChSeq, IP, WB

MAD1 antibody (mAb) (Clone 9B10)

Raised against amino acids 331-718 of mouse MAD1.

H, M

IF, WB

MAD2L1 antibody (mAb) (Clone 17D10)

Raised against full-length recombinant human MAD2L1.

H, M, WRWB

MALT1 antibody (mAb)

Raised against amino acids 701-808 of mouse MALT1 recombinant protein.

H

WB

MASH1 antibody (mAb) (Clone 24B7.2D11.1)

Raised against full-length recombinant rat MASH1.

M, R

IHC, WB

Maz antibody (mAb) (Clone 133)

Raised against full-length recombinant human Maz.

H

MBD1 antibody (mAb) (Clone 100B272.1)

Raised against a peptide corresponding to human MBD1.

H

ChIP, EMSA, IF,
IHC, IP, WB
WB

MBD1 antibody (pAb)

Raised against a peptide within the C-terminal region of human MBD1.

H

WB

MBD2 antibody (pAb)

Raised against a peptide corresponding to human MBD2.

H

WB

MBD3 antibody (mAb) (Clone 106B691)
MBD4 antibody (pAb)

Raised against a peptide corresponding to human MBD3.
Peptide mixture of amino acids 268-282 and 337-352 of human MBD4.

H
H

MeCP2 antibody (mAb) (Clone 4B4)Raised against full-length rat MeCP2 protein.

H, M, R

MeCP2 antibody (mAb) (Clone 4H7)

Raised against full-length rat MeCP2 protein.

H, M, R

MeCP2 antibody (pAb)

Peptide mixture of amino acids 11-25 and 181-195 of human MeCP2.

H

WB
WB
ChIP, IF, IHC,
IP, WB
ChIP, IF, IHC,
IP, WBWB

MeCP2 antibody (pAb)

Raised against the N-terminal 320 amino acids of recombinant mouse MeCP2.

H, M

WB

MeCP2 phospho Ser80 antibody (pAb)

Raised against a peptide with phospho-serine 80 of mouse MeCP2.

H, R

IHC, WB

MED1 antibody (pAb)

Raised against a peptide to the C-terminal region of human MED1.

H

WB

H, M, WR

FormatCatalog No.

100 µl10 µl100 µg200 µl10 µl100 µl10 µl100 µl10 µl100 µg10 µg200 µg10 µg200 µg10 µg100 µg10 µg100 µg10 µg100 µg100 µl10 µl200 µl10 µl100 µg100 µg100 µg10 µg100 µg10 µg100 µg200 µl10 µl100 µl10 µl100 µl
10 µl

61191
61192
40958
39186
39187
61175
61176
61177
61178
39659
39660
39399
39400
39395
39396
61271
61272
39935
39936
39215
39857
39858
39547
39548
39216
39217
61285
61286
61291
61292
39218
39188
39189
39733
39734
61065
61066

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

22

Antibody

Immunogen

Reactivity

MEIS 1/2/3 antibody (mAb) (Clone 9.2.7)

Raised against a peptide containing amino acids 50-390 of mouse MEIS1.

H, M

Menin antibody (pAb)

Raised against a peptide to the C-terminal region of human Menin.

H

MEP50 antibody (pAb)

Raised against His-tagged, full-length human MEP50.

MGMT antibody (pAb)

Applications

FormatCatalog No.

ChIP, EMSA,IHC, IP, WB
ChIP, ChCh,
ChSeq, WB

100 µg10 µg100 µl10 µl200 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg200 µl10 µl100 µg
10 µg

39795
39796
61005
61006
39190
39191
39951
39952
61463
61464
39695
39696
39289
39290
61143
61144

100 µg
10 µg

39789
39790

100 µg100 µg10 µg100 µg10 µg100 µl10 µl100 µg10 µg100 µl10 µl100 µl10 µl100 µg10 µg100 µg200 µl
10 µl

39219
39829
39830
61295
61296
61447
61448
39879
39880
61245
61246
39867
39868
39819
39820
39220
39361
39362

H, WR

WB

Raised against a peptide to the C-terminal region of human MGMT.

H

WB

Raised against a peptide corresponding to human Mi-2 beta.

DrIP, WB

Mi-2 beta antibody (mAb) (Clone 3F2/4)

Raised against a peptide corresponding to human Mi-2 beta.

H, M

IP, WB

Mi-2 beta antibody (pAb)

Raised against amino acids 22-155 of recombinant mouse Mi-2 beta.

H, M

IF, WB

Mili / PiwiL2 antibody (mAb) (Clone 17.8)

Raised against full-length, recombinant mouse Mili / PiwiL2 protein.

M

IF, IHC, IP, WB

MITF antibody (mAb)

Raised against an N-terminal fragment of human MITF.

H, M

MLH-1 antibody (mAb) (Clone 164C819)

Raised against a peptide corresponding to human MLH-1.
Raised against a recombinant protein containing amino acids 161-356 ofmouse MLL.
Raised against a recombinant protein containing amino acids 2829-2883 ofhuman MLL.

H

ChIP, ChCh, ChSeq,
EMSA, IF, IHC,
IP, WB
WB

H, M

ChIP, IP, WB

H, M

ChIP, IP, WB

H, M

WB

H

ChIP, ChCh, ChSeq, IF,
IP, WB

H

WB

H

WB

H

ICC, WB

H

WB

H, WR

WB

NEW Mi-2 antibody (mAb) (Clone 4D8)

MLL / HRX antibody (mAb) (Clone N4.4)
MLL / HRX antibody (pAb)
NEW MLL5 / KMT2E antibody (pAb)

MMSET / WHSC1 antibody (mAb) (Clone 29D1)
MOF / MYST1 antibody (pAb)
MOZ antibody (pAb)

Raised against a peptide to human MLL5 / KMT2E.
Raised against a recombinant protein containing amino acids 1-647 of human
MMSET-I.
Raised against a peptide to the N-terminal region of human MOF / MYST1(MYST histone acetyltransferase 1).
Raised against a peptide within the N-terminal half of MOZ.

MRE11 antibody (pAb)

Raised against a recombinant protein containing amino acids 50-120 of human
MPP8.
Raised against a peptide corresponding to human MRE11.

MRG15 antibody (pAb)

Raised against a peptide to the C-terminus of human MRG15.

MPP8 antibody (mAb) (Clone N119/44)

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

23

Antibody

Immunogen

Reactivity

Applications

MTA2 antibody (pAb)

Raised against a peptide to the C-terminus of human MTA2.

H, M, WRWB

Myc Tag antibody (mAb) (Clone 4E12)

Raised against a peptide to amino acids 410-419 of human c-Myc.

WR

WB

H

EMSA, IP, WB

c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)
c-Myc antibody (pAb) (65 kDa form)

Raised against a recombinant protein containing amino acids 1-262 ofhuman c-Myc.
Raised against amino acids 408-420 of human c-Myc; 61 kDa form.
Raised against amino acids 9-22 of human c-Myc; detects 65 kDa form.

H
H, Mk, M

WB
WB

N-Myc antibody (pAb)

Raised against a peptide within the C-terminal region of human N-Myc.

H

WB

MyD88 antibody (pAb)

Raised against amino acids 233-248 of human MyD88.

H

WB

Myf-5 antibody (mAb) (Clone 1E2G4)

Raised against a peptide derived from mouse Myf-5.

M

IF, WB

MyoD antibody (mAb) (Clone 5F11)

Raised against full-length, recombinant mouse MyoD protein.

H, M

IF, WB

NAK1/Nur77 antibody (pAb)

Raised against amino acids 251-266 of human NAK1/Nur77.

H, M

WB

Nanog antibody (pAb)

Raised against a peptide within the N-terminal region of mouse Nanog.

M

WB

Nap1 antibody (pAb)

Raised against full-length, His-tagged Drosophila Nap1 protein.

Dr

IP, WB

NAP1L1 antibody (pAb)

Raised against a peptide within the N-terminal region of NAP1L1.

H

WB

NBS1 antibody (pAb)

Raised against a peptide to the C-terminus of human NBS1.

H

WB

NcoA4 / ELE1 antibody (pAb)

Raised against a peptide to the C-terminal region of human NCoA4.

HWB

NF-1A antibody (pAb)

Raised against amino acids 478-492 of human NF-1A.

H

WB

NF-1B2 antibody (pAb)
NFkB p50 antibody (pAb)
NFkB p65 antibody (mAb) (Clone 112A1021)

Raised against amino acids 402-415 of human NF-1B2.
Raised against amino acids 363-376 of human NFkB p50.
Raised against amino acids 526-539 of human NFkB p65.

H, R
H, M
H, M

WB
WB
FC, WB

NFkB p65 antibody (pAb)

Raised against a peptide immunogen to N-terminal NFkB p65.

H

WB

NFkB p65 antibody (pAb)

Raised against a peptide to the C-terminus of human NFkB p65.

H

ChIP, WB

NFkB p65 phospho Ser529 antibody (pAb)

Raised against a peptide containing phospho-serine 529 of human NFkB p65.

H

DB, WBc-Myc antibody (mAb) (Clone 6A10)

FormatCatalog No.

200 µl10 µl200 µg10 µg100 µg10 µg100 µl100 µl100 µl10 µl100 µg100 µg10 µg100 µg10 µg200 µl100 µl10 µl100 µl100 µl10 µl200 µl10 µl100 µl10 µl200 µl10 µl100 µg200 µl100 µg200 µl10 µl200 µl10 µl100 µl
10 µl

39359
39360
39279
39280
61075
61076
39502
39012
61185
61186
40914
39801
39802
39991
39992
40982
61419
61420
39577
39841
39842
39373
39374
39895
39896
39397
39398
39091
39037
40916
39283
39284
39369
39370
39691
39692

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

24

Antibody

Immunogen

Reactivity

Applications

NFkB p65 phospho Ser536 antibody (pAb)

Raised against a peptide containing phospho-serine 536 of human NFkB p65.

H

DB, WB

NFkB p100 antibody (pAb)

Raised against a peptide to the C-terminus of human NFkB p100.

H

WB

NKX2.5 antibody (pAb)

Raised against a peptide within the N-terminal region of human NKX2.5(NK2 homeobox 5).

H

WB

NonO antibody (pAb)

Raised against a peptide to the C-terminus of human NonO.

H

WB

H, R

WB

H

WB

FormatCatalog No.
39675
39676
39687
39688
61267
61268
61247
61248
61147
61148
61149
61150
61279
61280
61213
61214
39541
39542
39849
39850
61453
61454
39811
39812
61425
61426
61355
61356

NR2C2 antibody (pAb)

Raised against a peptide within the N-terminal region of human NR2C2.

HWB

NR2F2 / COUP2 antibody (pAb)

Raised against a peptide within the N-terminal region of human NR2F2.

H

ChIP, ChCh,
ChSeq, WB

Nucleolin antibody (mAb) (Clone 3G4B20)

Raised against a salt-extracted nuclear matrix preparation from MDCK cells.

H, M, WR

IF, WB

Nucleophosmin antibody (pAb)

Raised against a peptide within the C-terminal region of human nucleophosmin.

H

WB

Raised against amino acids 20-37 of human Osteoprotegerin (OPG).

NS

IP, WB

Raised against a peptide derived from human Oct-4.

H

WB

H

WB

H

ChIP, ChCh,
ChSeq, WB

100 µl10 µl100 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg100 µg10 µg100 µl10 µl100 µl10 µl200 µl10 µl100 µl10 µl100 µg10 µg100 µl10 µl100 µl10 µl100 µl10 µl100 µg40938

200 µg
10 µg
17 rxns
100 µg100 µl10 µl100 µg100 µg

39553
39554
39334
40974
39739
39740
40972
40973

Notch1 antibody (mAb) (Clone ICN 5B5)
Notch3 antibody (mAb) (Clone NIZ 8G5)

NEW O-GlcNAc antibody (mAb) (Clone CTD110.6)

Oct-4 antibody (pAb)
OGA/O-GlcNAcase antibody (pAb)
OGT/O-GlcNAc transferase antibody (pAb)

Raised against recombinant protein corresponding to the intracellular domainof human Notch1 protein.
Raised against recombinant protein corresponding to the intracytoplasmic
portion of human Notch1 protein.

Raised against a recombinant protein containing the N-terminal region ofhuman OGA.
Raised against a recombinant protein containing the N-terminal region ofhuman OGT.

Osteoprotegerin (OPG) antibody (mAb)(Clone 98A1071)

Raised against amino acids 20-37 of human Osteoprotegerin (OPG).

H

WB

p53 antibody (mAb) (Clone DO1)

Raised against full-length recombinant human p53 protein.

HIP, WB

p53 antibody (pAb)
p63 antibody (mAb) (Clone 87C553)

Raised against amino acids 367-381 of human p53.
Raised against a peptide corresponding to human p63.

H, M
H

ChIP, EMSA
WB

p63 antibody (pAb)

Raised against a recombinant full-length human p63.

H, M

ChIP, IF, WB

p73 antibody (mAb) (Clone 5B429)
p73DN antibody (mAb) (Clone 38C674)

Raised against full-length human p73.
Raised against amino acids 2-13 of human p73DN.

H
H

WB
WB

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

25

Antibody

Immunogen

Reactivity

Applications

FormatCatalog No.

p300 antibody (mAb) (Clone NM11)

Raised against full-length p300 purified from human 293 cells.

H

ChIP, ChCh,
ChSeq, IF, IP, WB

PARP-1 N-terminal antibody (pAb)

Raised against the His-tagged, N-terminal half of human PARP-1.

H

ChIP, IHC, IP, WB

PARP-1 C-terminal antibody (pAb)

Raised against the His-tagged, C-terminal half of human PARP-1.

H

ChIP, IHC, IP, WB

PARP-2 antibody (pAb)

Raised against a recombinant C-terminal region of human PARP-2.

H

ChIP, IP, WB

PAX7 antibody (mAb) (Clone 2F12H4)

Raised against a peptide derived from mouse Pax-7.

M

IF, WB

PAX9 antibody (mAb) (Clone 7C2)

Raised against recombinant protein containing amino acids 263-342 of mouse
PAX9.

H

IHC, IP, WB

PBRM1 antibody (pAb)

Raised against a peptide within the N-terminal region of human PBRM1.

H

WB

H

WB

H

ChIP, IHC, IP, WB

H

IHC, WB

H

WB

100 µg10 µg200 µl10 µl200 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg100 µl10 µl100 µg10 µg100 µg10 µg100 µg10 µg100 µg10 µg100 µg10 µg100 µl10 µl100 µl10 µl100 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg100 µl
10 µl

61401
61402
39559
39560
39561
39562
39743
39744
39803
39804
61077
61078
61381
61382
61151
61152
61165
61166
61155
61156
61153
61154
61079
61080
61289
61290
61263
61264
39859
39860
61217
61218
39723
39724
39661
39662
39711
39712

PBX, long form antibody (mAb) (Clone 359.19)
PBX1b antibody (mAb) (Clone CT 41.1)
PBX2 antibody (mAb) (Clone 2.1)
PCL2 antibody (mAb) (Clone 3A9)

Raised against a recombinant protein containing amino acids 319-430 of human
PBX1A.
Raised against a recombinant protein containing amino acids 319-347 of human
PBX1b.
Raised against a recombinant protein containing amino acids 308-430 of human
PBX2.
Raised against a recombinant protein containing amino acids 103-327 of mouse
PCL2.

PCNA antibody (mAb) (Clone 16D10)

Raised against full-length recombinant human PCNA.

H

IF, IP, WB

PDX1 antibody (pAb)

Raised against a peptide within the N-terminal region region of human PDX1.

HWB

PELP1 antibody (pAb)

Raised against a peptide within the internal region of human PELP1.

H

WB

PGC1-b antibody (pAb)

Raised against a peptide within the N-terminal region of human PGC1-b.

H

WB

PHB / Prohibitin antibody (pAb)

Raised against a peptide within the N-terminal region of human PHB.

H

WB

Phc1 antibody (mAb) (Clone 6-1-3)

Raised against full-length, recombinant mouse Phc1.

H

IF, IP, WB

Phc2 antibody (mAb)

Raised against a recombinant fragment of human Phc2.

H, M

ChIP, IF, IP

PHF8 antibody (pAb)

Raised against a peptide derived from human PHF8.

H

WB

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

26

Antibody

Immunogen

Reactivity

Applications

PHF20 antibody (pAb)

Raised against a peptide derived from human PHF20.

HWB

PIM2 antibody (mAb) (Clone CHU61)

Raised against recombinant human PIM2 protein.

H

IHC, IP, WB

PITX2 antibody (pAb)

Raised against a peptide within the N-terminal region of human PITX2.

HWB

PKM2 antibody (pAb)

Raised against a peptide to the C-terminal region of human PKM2.

HWB

PLZF antibody (mAb) (Clone 2A9)

Raised against full-length, recombinant mouse PLZF protein with a GST tag.

H, M

ChIP, IF, WB

PML antibody (mAb) (Clone 36.1-104)

Raised against amino acids 1-581 of mouse PML.

M

IF, IP, WB

PMS2 antibody (mAb) (Clone 163C1251)

Raised against amino acids 623-639 of human PMS2.

H, M

WB

PP2A antibody (pAb)

Raised against amino acids 288-303 of human PP2A.

H, M, R, WR

ChIP, IP, WB

PPAR-g antibody (mAb) (Clone 3PP-1H4)

Raised against a peptide within the N-terminal region of human PPAR-g1 and g2.

H

IF, WB

Progesterone Receptor antibody (mAb) (Clone 6A) Raised against a peptide to the C-terminus of human Progesterone Receptor.

H

ChIP, IF, IHC, WB

PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)

Raised against full-length, recombinant human PRDM1 / BLIMP1 protein.

H

FC, IHC, IP, WB

NEW PRMT2 antibody (pAb)

Raised against a peptide within the N-terminal region of human PRMT2.

HWB

PRMT5 antibody (pAb)

Raised against a peptide within the N-terminal region of human PRMT5.

HWB

PRMT6 antibody (pAb)

Raised against a peptide within the N-terminal region of human PRMT6.

HWBProgerin antibody (mAb) (Clone 13A4)

Raised against a peptide with amino acids 604-611 of human Progerin.

HIF, IP, WB

Prox1 antibody (mAb) (Clone 4G10)

Raised against a recombinant protein with amino acids 547-737 of human Prox1.

H

WB

Prox1 antibody (mAb) (Clone 5G10)

Raised against a recombinant protein with amino acids 547-737 of human Prox1.

H

IF, IHCPXR antibody (pAb)

Raised against a peptide with amino acids 51-71 of human PXR.

HWBRad1 antibody (pAb)
Rad17 phospho Ser647 antibody (pAb)
Rad18 antibody (mAb) (Clone 79B1048)

Raised against amino acids 133-148 of human Rad1.
Raised against a peptide with phospho-serine 647 of mouse Rad17.
Raised against amino acids 402-414 of human Rad18.

H
H, R
H

WB
IF, WB
WB

FormatCatalog No.

100 µl10 µl100 µg10 µg100 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg100 µg200 µl10 µl100 µg10 µg100 µg10 µg100 µg10 µg100 µl10 µl100 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg100 µg10 µg200 µl10 µl100 µg100 µg100 µg

61123
61124
61159
61160
61303
61304
61423
61424
39987
39988
39773
39774
39221
39192
39193
61025
61026
61023
61024
61167
61168
61491
61492
61001
61002
61003
61004
39965
39966
61091
61092
61093
61094
39589
39590
40978
39222
40979

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

27

Antibody

Immunogen

Reactivity

Applications

Rad21 antibody (mAb) (Clone 52A311)

Recombinant protein from the N-terminal half of mouse Rad21.

H, M

WB

Rad51 antibody (pAb)

Raised against full-length human Rad51 expressed in E. coli.

H

IF, IP, WB

Rad52 antibody (pAb)

Raised against full-length human Rad52 expressed in E. coli.

H

IF, IHCRANK antibody (mAb) (Clone 9A725)

Raised against amino acids 326-616 of GST-tagged, human RANK.

HWB

RAP1 / TERF2IP antibody (mAb) (Clone 4C8)

Raised against full-length human RAP1 / TERF2IP.

H

WB

RAP1 / TERF2IP antibody (pAb)

Raised against a peptide within human RAP1 / TERF2IP.

H

WB

RAR-a antibody (mAb) (Clone 9a-9A6)

Raised against recombinant protein with amino acids 420-443 of human RAR-a.

H, M

ELISA, EMSA, ICC,
IHC, IP, WB

RAR-b antibody (mAb) (Clone 8B-10B2)

Raised against amino acids 413-448 of human RAR-b.

H

EMSA, IHC, IP, WB

RAR-g antibody (mAb) (Clone 4g-7A11)

Raised against recombinant protein with amino acids 428-441 of human RAR-g.

H

ELISA, EMSA,
ICC, IP, WB

RbAp46/48 antibody (pAb)

Raised against recombinant human RbAp46 protein.

H, WR

ChIP, WBRBBP5 antibody (pAb)

Raised against a peptide within the C-terminal region of human RBBP5.

HWB

NEW RBPJ antibody (mAb) (Clone 1F1)

Raised against full-length recombinant human RBPJ protein.

H

ChIP, ChCh, ChSeq,
EMSA, IPRBPJ antibody (mAb) (Clone 7A11)

Raised against full-length recombinant human RBPJ protein.

H

WB

Ring1B antibody (mAb)

Raised against recombinant full-length human Ring1B.

RNA pol II antibody (mAb) (Clone 4H8)

Raised against a peptide containing YSPTSpPS from human RNA pol II.

RNA Pol II antibody (mAb) (Clone 4F8)

Raised against peptide with human RNA Pol II C-terminal heptad repeat domain.

RNA Pol II CTD phospho Tyr1 antibody (mAb)(Clone 3D12)
RNA Pol II CTD phospho Ser2 antibody (mAb)(Clone 3E10)

Raised against a peptide with the RNA Pol II CTD sequence phosphorylated attyrosine 1.
Raised against a peptide with the RNA Pol II CTD sequence phosphorylated atserine 2.
Raised against a peptide with the RNA Pol II CTD sequence phosphorylated atserine 2.

RNA Pol II CTD phospho Ser2 antibody (pAb)

H, Mk, M
BY, Ce, H, M, R

ChIP, ChCh, ChSeq, IF,
IP, WB
ChIP, ChCh, ChSeq,
ELISA, IF, IP, WB

H, M

ChIP, IF, IP, WB

H, WR

ChIP, IP, WB

H, M

ChIP, ChCh, ChSeq, IF,
IP, WBH

DB, WB

FormatCatalog No.

200 µg10 µg200 µg10 µg200 µg10 µg100 µg100 µg10 µg100 µl10 µl100 µg10 µg100 µg10 µg100 µg10 µg200 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg100 µg
10 µg

39383
39384
39194
39195
39196
39197
40917
61235
61236
61069
61070
39971
39972
61027
61028
39973
39974
39198
39199
61405
61406
61505
61506
61073
61074
39663
39664

200 µl39097

100 µg10 µg100 µg10 µg100 µg10 µg200 µl
10 µl

61081
61082
61383
61384
61083
61084
39563
39564

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

28

Antibody

Immunogen

RNA Pol II CTD phospho Ser7 antibody (mAb)(Clone 4E12)
Rsf1 antibody (pAb)

Raised against a peptide with the RNA Pol II CTD sequence phosphorylated at
threonine 4.
Raised against a peptide with the RNA Pol II CTD sequence phosphorylated at
threonine 4.
Raised against a peptide with the RNA Pol II CTD sequence phosphorylated atserine 5.
Raised against a peptide with RNA Pol II heptad repeat phosphorylated atserine 5. (Rabbit serum)
Raised against a peptide with RNA Pol II heptad repeat phosphorylated atserine 5. (Purified rabbit IgG)
Raised against a peptide with the RNA Pol II CTD sequence phosphorylated atserine 7.
Raised against His-tagged protein with amino acids 165-350 Drosophila Rsf1.

RXR-a antibody (mAb) (Clone 4RX-3A2)

RNA Pol II CTD phospho Thr4 antibody (pAb)
RNA Pol II CTD phospho Thr4 antibody (mAb)(Clone 6D7)RNA Pol II CTD phospho Ser5 antibody (mAb)(Clone 3E8)
RNA pol II CTD phospho Ser5 antibody (pAb)

Reactivity

Applications

H

WB

H, WR

ChIP, IF, WB

H

ChIP, ChCh, ChSeq, IF,
IP, WB

H, M, WR

ChIP, DB, IF, WB

Dr

ChIP, ChCh, ChSeq,
DB, WB
ChIP, ChCh, ChSeq, IF,
IP, WBIF, IP, WB

Raised against recombinant protein to the C-terminal region of mouse RXR-a.

H

EMSA, IP, WB

RXR-b antibody (mAb) (Clone 16RX-2E1)

Raised against peptide to the N-terminal region of mouse RXR-b.

H

EMSA, IF, IHC, WB

RXR-g antibody (mAb) (Clone 12RX-2D3)

Raised against peptide within the D-region region of mouse RXR-g.

H

EMSA, IHC, WB

Raised against a peptide with the C-terminal region of human RYBP.

H, M

WB

SALL4 antibody (pAb)

Raised against a peptide to the N-terminus of human SALL4.

M

WB

SAP30 antibody (pAb)

Raised against recombinant full-length human SAP30.

H

ChIP, ChCh,
ChSeq, IP, WB

SATB1 antibody (pAb)

Raised against a peptide within the N-terminal region of SATB1.

H, M

WB

Raised against a peptide to human SET7D / SET7.

HWBSET8 / PR-SET7 antibody (pAb)

Raised against a peptide to the N-terminal half of SET8 / PR-SET7.

H

WB

Sf3b3 antibody (mAb) (Clone 1YO-1B10)

Raised against a peptide containing amino acids 1057-1076 of human Sf3b3.

H, M

IP, WB

SIN3A antibody (pAb)

Raised against a peptide to the N-terminus of SIN3A.

H, M

ChIP, IP, WB

SIP1 antibody (mAb) (Clone 6E5)

Raised against recombinant protein corresponding to amino acids 1-360 ofhuman SIP1.

H

IF, IHC, IP, WB

RNA pol II CTD phospho Ser5 antibody (pAb)

NEW RYBP antibody (pAb)

NEW SETD7 / SET7 antibody (pAb)

H, M, WR
H

FormatCatalog No.

100 µg10 µg100 µg10 µg100 µg10 µg200 µl10 µl100 µg10 µg100 µg10 µg100 µl100 µg10 µg100 µg10 µg100 µg10 µg100 µl10 µl100 µl10 µl100 µl10 µl100 µl10 µl100 µl10 µl100 µl10 µl100 µg10 µg100 µl10 µl100 µg
10 µg

61307
61308
61361
61362
61085
61086
39233
39234
39749
39750
61087
61088
39579
61029
61030
61031
61032
61033
61034
61461
61462
39957
39958
39731
39732
39839
39840
61451
61452
61009
61010
61345
61346
39865
39866
61095
61096

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

29

Antibody

Immunogen

Reactivity

Applications

SIP1 antibody (pAb)

Raised against a peptide to the N-terminus of human SIP1.

HWB

SIRT1 antibody (mAb) (Clone 2G1/F7)

Raised against amino acids 581-630 of human SIRT1.

H

IF, IP, WB

SIRT2 antibody (pAb)

Raised against a peptide to the C-terminus of human SIRT2.

HWB

SIRT2 antibody (pAb)

Raised against a peptide to the C-terminus of human SIRT2.

H

IF, IP, WB

SIRT2 antibody (pAb)

Raised against a peptide to the C-terminus of mouse SIRT2.

M

IF, WBSIRT2 antibody (mAb) (Clone 7G5)

Raised against a peptide to human SIRT2 surrounding Ser331. This antibody
recognizes both the unphosporylated and the phospho-Ser331 forms of SIRT2.

HWB

SIRT2 phospho Ser331 antibody (mAb) (Clone 6B5)

Raised against a peptide with phospho-serine 331 of human SIRT2.

HWB

SIRT6 antibody (pAb)

Raised against a peptide to the C-terminus of human SIRT6.

H

WB

H

WB

H

IHC, WB

H, M

ChIP, IF, WB

H, M

IF, WB

B, H, M

IP, WB

H

WB

H, M

WB

SMAD3 antibody (pAb)
NEW SMARCA1 / SNF2L1 antibody (mAb) (Clone 2C4)SMARCA2 / BRM antibody (mAb) (Clone 1H7A10)SMARCA4 / BRG1 antibody (mAb) (Clone 3G4)
SMARCAL1 / HARP antibody (mAb) (Clone 4B10)
SMARCC1 / BAF155 antibody (pAb)
NEW SMARCC2 / BAF170 antibody (pAb)

Raised against a peptide within human SMAD3 (mothers against
decapentaplegic homolog 3).
Raised against a recombinant protein containing amino acids 571-772 ofhuman SNF2L
Raised against a recombinant protein containing amino acids 48-214 ofmouse BRM.
Raised against a recombinant protein containing amino acids 214-279 ofmouse BRG1.
Raised against purifed bovine SMARCAL1 / HARP protein.
Raised against a peptide within the N-terminal region ofhuman SMARCC1 / BAF155.
Raised against a peptide within the C-terminal region ofhuman SMARCC2 / BAF170.

SMC1-a antibody (pAb)

Raised against a peptide to the C-terminus of human SMC1-a.

H

WB

SMC1 phospho Ser957 antibody (pAb)

Raised against a peptide with phospho-serine 957 of human SMC1.

H

DB, WB

SMC3 antibody (pAb)

Raised against a peptide to the C-terminal region of human SMC3.

HWB

SMC4 antibody (pAb)

Raised against a peptide to the N-terminus of human SMC4H

WB

FormatCatalog No.

100 µl10 µl200 µg10 µg100 µl10 µl100 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg100 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg100 µg10 µg100 µg10 µg100 µl10 µl100 µl10 µl100 µl10 µl200 µl10 µl100 µl10 µl100 µl
10 µl

61113
61114
39353
39354
39903
39904
61043
61044
61045
61046
61365
61366
61363
61364
39911
39912
61249
61250
61465
61466
39805
39806
39807
39808
61115
61116
61409
61410
61471
61472
61067
61068
39631
39632
61131
61132
61129
61130

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

30

Antibody

Immunogen

Reactivity

Applications

SMRT / NCoR2 antibody (mAb) (Clone 5.10)

Raised against a recombinant protein with amino acids 2004-2310 of human
SMRT / NCoR2.

HWB

SMRT / NCoR2 antibody (pAb)

Raised against a peptide within the N-terminal region of SMRT / NCoR2.

H

WB

Smyd3 antibody (pAb)

Raised against a peptide against human Smyd3.

H

WB

SNAI1 / Snail antibody (mAb) (Clone 9H2)

Raised against full-length, recombinant human SNAI1 protein.

H

IHC, WB

Raised against a recombinant protein with amino acids 505-674 of human SND1.

H, M

IF, IHC, IP, WB

SNF2h antibody (mAb)

SNF2h+ clones were selected from purified RSF complex-immunized mice.

H, M

ChIP, IF, IP, WB

SNF5 antibody (pAb)

Raised against a peptide to the C-terminus of human SNF5.

H

WB

Sox2 antibody (pAb)

Raised against a peptide containing amino acids 111-140 of mouse Sox2.

H

ChIP, IF, IHC, IP, WB

Sox2 antibody (pAb)

Raised against a peptide to the C-terminal region of human Sox2.

H

WB

Sox11 antibody (pAb)

Raised against a peptide to the N-terminus of human Sox11.

H

WB

Sp1 antibody (pAb)

Raised against amino acids 520-534 of human Sp1.
Raised against a peptide with serine 101 of human Sp1. This antibody recognizes
human Sp1 when it is not phosphorylated at serine 101.

H, M, R

ChIP, IP, WB

H

WB

H

DB, WBH

IP, WB

H

ChIP, IP, WB

NEW SND1 antibody (mAb) (Clone SN4-1)

Sp1 non-phospho antibody (pAb)
Sp1 phospho Ser101 antibody (pAb)
SREBP-1 antibody (mAb) (Clone IgG-2A4)
SREBP-2 antibody (mAb) (Clone IgG-1C6)

Raised against a peptide with phospho-serine 101 of human Sp1.
Raised against a recombinant protein with amino acids 301-407 of human
SREBP-1.
Raised against a recombinant protein with amino acids 833-1141 of human
SREBP-2.

SRF antibody (mAb) (Clone 2C5)

Raised against full-length mouse SRF protein.

H, M

IHC, WBSRF Drosophila antibody (mAb)

Raised against full-length Drosophila SRF.

Dr

WB

Raised against a peptide to the N-terminus of human SSRP1.

HWB

STAT1 phospho Ser727 antibody (pAb)

Raised against a peptide with phospho-serine 727 of human STAT1.

H

DB, WBSTAT1a antibody (pAb)

Raised against amino acids 712-750 of human STAT1a.

H

WB

NEW SSRP1 antibody (pAb)

FormatCatalog No.

100 µg10 µg100 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg200 µl10 µl100 µl10 µl100 µl10 µl100 µl10 µl100 µl10 µl100 µl100 µl10 µl100 µl10 µl100 µg10 µg100 µg10 µg100 µg10 µg200 µl100 µl10 µl200 µl10 µl100 µl

61105
61106
39845
39846
61407
61408
61367
61368
61473
61474
39543
39544
39775
39776
39823
39824
39843
39844
61181
61182
39058
39809
39810
39757
39758
39939
39940
39941
39942
61385
61386
39093
61455
61456
39633
39634
39059

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

31

Antibody

Immunogen

Reactivity

Applications

STAT2 phospho Tyr689 antibody (pAb)

Raised against a peptide with phospho-tyrosine 689 of human STAT2.

H

DB, WBSTAT3 phospho Tyr705 antibody (pAb)

Raised against a peptide with phospho-tyrosine 705 of human STAT3.

H

DB, WBSTAT3 phospho Ser727 antibody (pAb)

Raised against a peptide with phospho-tyrosine 727 of human STAT3.

H

DB, WBNEW STAT4 antibody (pAb)

Raised against a peptide to the C-terminal region of human STAT4.

HWBSTAT5A antibody (pAb)

Raised against a peptide to the C-terminal region of human STAT5A.

H

WB

H

DB, WBH

DB, WBSTAT5A/B phospho Tyr694/Tyr699 antibody (pAb)
STAT5A/B phospho Ser726/Ser731 antibody (pAb)

Raised against a peptide with phospho-tryosine 694 of human STAT5A.
(Human STAT5B shares the identical sequence surrounding Tyr699.)
Raised against a peptide with phospho-serine 726 of human STAT5A.
(Human STAT5B shares the identical sequence surrounding Ser731.)

STAT5B antibody (pAb)

Raised against a peptide to the C-terminal region of human STAT5B.

H

WB

Supt3h antibody (mAb) (Clone 1SP-2C10)

Raised against a peptide containing amino acids 10-27 of human Supt3h.

H

ChIP, IP, WB

Supt16H / Spt16 antibody (pAb)

Raised against a peptide containing human Supt16H / Spt16.

H

WB

SUV39H1 antibody (mAb) (Clone MG44)

Raised against a recombinant protein with amino acids 1-126 of mouse SUV39H1.

H, M

ChIP, IP, WB

NEW SUV39H2 antibody (pAb)

Raised against a peptide to the C-terminal region of mouse SUV39H2.

H, M

WB

SUV420H1 antibody (pAb)

Raised against a peptide to the N-terminal region of human SUV420H1.

H

WB

Suz12 antibody (mAb) (Clone 2A09)

Raised against full-length recombinant human Suz12.

H

ChIP, IF, IHC, IP

Suz12 antibody (pAb)

Raised against a peptide to the C-terminus of human Suz12.

H, M

ChIP, IP, WB

TACI antibody (pAb)

Raised against amino acids 116-132 of human TACI.

H, M

FC, WB

Tada2a antibody (mAb) (Clone 2AD-2A1)

Raised against a peptide containing amino acids 1-17 of human Tada2a.

H

IP

TAL-1 antibody (mAb) (Clone BTL73)

Raised against full-length recombinant human TAL-1 protein.

H

EMSA, IHC, WB

TAZ / WWTR1 antibody (pAb)

Raised against a peptide to the C-terminal region of human TAZ / WWTR1.

H

WB

TBK1 antibody (mAb) (Clone 108A429)

Raised against amino acids 563-577 of human TBK1.

H, M, RWB

FormatCatalog No.

200 µl10 µl200 µl10 µl200 µl10 µl100 µl10 µl100 µg10 µg200 µl10 µl200 µl10 µl100 µg10 µg100 µg10 µg100 µl10 µl100 µg10 µg100 µl10 µl100 µl10 µl100 µg10 µg200 µl10 µl100 µg100 µg10 µg100 µg10 µg100 µl10 µl100 µg

39611
39612
39595
39596
39613
39614
61507
61508
61133
61134
39617
39618
39597
39598
61137
61138
61343
61344
61441
61442
39785
39786
61449
61450
61415
61416
39877
39878
39357
39358
40936
61333
61334
61259
61260
61265
61266
40909

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

32

Antibody

Immunogen

Reactivity

Applications

Tbp antibody (mAb) (Clone 3TF1-3G3)

Raised against a recombinant protein corresponding to human Tbp.

H, M

IF, IHC, IP, WB

Tbpl1 antibody (mAb) (Clone 3TRF-2A1)

Raised against a recombinant protein corresponding to mouse Tbpl1.

H, M

IHC, WBTCF7L1 / TCF3 antibody (pAb)

Raised against a peptide to the N-terminus of human TCF7L1 / TCF3.

HWB

TDG / Thymine-DNA glycosylase antibody (pAb)

Raised against full-length recombinant mouse thymine DNA glycosylase (TDG).

M

WB

Raised against a peptide to the C-terminal region of mouse Tet1.

H, M

ChIP, ChCh,
ChSeq, WB

Tet2 antibody (mAb) (Clone 21F11)

Raised against a recombinant protein with amino acids 1-300 of human Tet2.

H

IP, WB

Tet3 antibody (pAb)

Raised against a recombinant protein with amino acids 1159-1329 of mouse Tet3.

M

WB

TLR2 antibody (pAb)
TLR3 antibody (mAb) (Clone 40C1285)
TLR6 antibody (mAb) (Clone 86B1153)
TLR8 antibody (mAb) (Clone 44C143)
TLR9 antibody (mAb) (Clone 26C593)

Peptide mix of amino acids 180-196, 353-370 & 473-489 of human TLR2.
Raised against a KLH-peptide to human TLR3.
Raised against amino acids 408-424 of human TLR6.
Raised against amino acids 750-850 of human TLR8.
Raised against a KLH-peptide to amino acids 268-284 of human TLR9.

H
H
H
H, M
H, M, RWB
FC, IHC, IP, WB
FC, WB
FC, WBFC, IHC, WB

TORC2 antibody (pAb)

Raised against a peptide to the C-terminus of human TORC2.

H, M

WB

TRAF2 antibody (mAb) (Clone 33A1293)
TRAF5 antibody (mAb) (Clone 55A219)
TRAIL antibody (mAb) (Clone 55B709.3)
TRANCE/RANKL antibody (mAb) (Clone 12A380)
TRF2 antibody (pAb)

Raised against amino acids 215-222 of human TRAF2 protein.
Raised against amino acids 77-186 of human TRAF5 protein.
Raised against amino acids 17-35 of human TRAIL.
Raised against amino acids 1-317 of GST-tagged, human TRANCE/RANKL.
Raised against full-length human TRF2 protein.

H
H
H
H, M
H, M, RWB
WBFC, WBFC, IHC, WB
ChIP, IP, WB

TRIM28 antibody (mAb) (Clone 1Tb-1A9)

Raised against full-length mouse TRIM28 protein.

H

ChIP, IF, IP, WB

TROY/TAJ antibody (pAb)

Peptide mix of amino acids 29-44, 204-219 & 409-421 of human TROY/TAJ.

H, M

WB

Trrap antibody (mAb) (Clone 2TRR-1B3)

Raised against a peptide containing amino acids 2023-2042 of human Trrap.

H, M

ChIP, IPTWIST antibody (mAb)

Raised against full-length recombinant mouse TWIST protein.

H, M

IF, IHC, WB

UBE2N antibody (pAb)
UBE2V1a antibody (pAb)
UBE2V2 antibody (pAb)

Peptide mixture of amino acids 2-19 and 131-148 of human UBE2N.
Raised against amino acids 31-49 of human UBE2V1a.
Raised against amino acids 12-28 of human UBE2V2.

H
H
H

IP, WBWBWBNEW Tet1 antibody (pAb)

FormatCatalog No.

100 µg10 µg100 µg10 µg100 µl10 µl100 µl10 µl100 µl10 µl100 µg10 µg100 µl10 µl100 µg100 µg100 µg100 µg100 µg100 µl10 µl100 µg100 µg100 µg100 µg100 µg100 µg10 µg100 µg100 µg10 µg100 µg10 µg100 µg100 µg100 µg

61329
61330
61331
61332
61125
61126
61437
61438
61443
61444
61389
61390
61395
61396
40981
40952
40954
40955
40956
39779
39780
40919
40920
40966
40940
39223
61173
61174
40937
61335
61336
61097
61098
39224
39225
39226

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

33

Antibody

Immunogen

Reactivity

Applications

Ubiquitin antibody (mAb) (Clone P4D1)

Raised against purified bovine ubiquitin.

WR

IF, IHC, IP, WB

Ubn1 antibody (pAb)

Raised against amino acids 1-345 of human Ubn1.

H

WB

Uhrf1 antibody (mAb) (Clone 1RC-1C10)

Raised against a peptide containing amino acids 263-793 of human Uhrf1.

H

IF, IHC, IP, WB

Uhrf1 antibody (pAb)

Raised against a peptide to the C-terminus of human Uhrf1.

H

WB

UTF1 antibody (pAb)

Raised against a peptide within the N-terminal region of human UTF1.

H, M

WB

NEW WDR5 antibody (pAb)

Raised against a peptide within the C-terminal region of human WRD5.

H

WB

H

IF, IHC, WB

H

WB

H

WB

H, Mk, M, R

ChIP, IP, WB

HWB

WRN antibody (mAb) (Clone 195C)
XRCC1 antibody (pAb)

Raised against a recombinant protein containing amino acids 1072-1432 of human
WRN.
Raised against a recombinant protein containing the N-terminal region ofhuman XRCC1.

YAP1 antibody (pAb)

Raised against a peptide within the C-terminal region of human YAP1.

YY1 antibody (pAb)

Raised against amino acids 109-123 of human YY1.

ZEB1 antibody (pAb)

Raised against a peptide to the N-terminal region of human ZEB1.

FormatCatalog No.

100 µg10 µg100 µl10 µl100 µg10 µg200 µl10 µl100 µl10 µl100 µl10 µl100 µg10 µg100 µl10 µl100 µl10 µl100 µl100 µl
10 µl

39741
39742
39869
39870
61341
61342
39625
39626
61253
61254
61485
61486
61169
61170
61371
61372
61293
61294
39071
61119
61120

Reactivity: B = Bovine; BY = Budding Yeast; Ce = C. elegans; Dr = Drosophila; FY = Fission Yeast; Hm = Hamster; H = Human; Mk = Monkey; M = Mouse; NS = Not Species Specific; Rb = Rabbit; R = Rat; WR = Wide range predicted
Appl.: ChIP = Chromatin IP; ChCh = ChIP-chip; ChSeq = ChIP-Seq; DB = Dot Blot; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA IP; WB = Western Blot

www.activemotif.com/abs

34

Fluorescent and HRP Secondary Antibody Conjugates
The table below provides details on all of Active Motif’s secondary antibody conjugates. For more comprehensive information on each, including data and downloadable
Technical Data Sheets, please visit our website at www.activemotif.com/secondary.
Name

Absorption

Emissione L/(mol-cm)

Stokes shift

FormatCatalog No.

—
—

—
—

—
—

—
—

ATTO 532 (GSD) Goat anti-Mouse IgG

534 nm

560 nm115,000

26 nm

ATTO 532 (GSD) Goat anti-Rabbit IgG

534 nm

560 nm115,000

26 nm

ATTO 594 Goat anti-Mouse IgG

601 nm

627 nm

120,000

26 nm

ATTO 594 Goat anti-Rabbit IgG

601 nm

627 nm

120,000

26 nm

ATTO 647N (STED/GSD) Goat anti-Mouse IgG

644 nm

669 nm150,000

25 nm

ATTO 647N (STED/GSD) Goat anti-Rabbit IgG

644 nm

669 nm150,000

25 nm

ATTO 655 (STED) Goat anti-Mouse IgG

663 nm

684 nm

125,000

21 nm

ATTO 655 (STED) Goat anti-Rabbit IgG

663 nm

684 nm

125,000

21 nm

Chromeo™ 488 Goat anti-Mouse IgG

498 nm524 nm73,00026 nm

Chromeo™ 488 Goat anti-Rabbit IgG

498 nm524 nm73,00026 nm

Chromeo™ 494 Goat anti-Mouse IgG

489 nm624 nm55,000135 nmChromeo™ 494 Goat anti-Rabbit IgG

489 nm624 nm55,000135 nmChromeo™ 505 Goat anti-Mouse IgG

514 nm

530 nm70,00016 nm

Chromeo™ 505 Goat anti-Rabbit IgG

514 nm

530 nm70,00016 nm

Chromeo™ 546 Goat anti-Mouse IgG

550 nm

567 nm

98,80017 nm

Chromeo™ 546 Goat anti-Rabbit IgG

550 nm

567 nm

98,80017 nm

400 µg
400 µg
250 µl35 µl
250 µl35 µl
250 µl35 µl
250 µl35 µl
250 µl35 µl
250 µl35 µl
250 µl35 µl
250 µl35 µl
1 mg
100 µg
1 mg
100 µg
1 mg
100 µg
1 mg
100 µg
1 mg
100 µg
1 mg
100 µg
1 mg
100 µg
1 mg
100 µg

15014
15015
15070
15071
15072
15073
15037
15057
15047
15067
15038
15058
15048
15068
15039
15059
15049
15069
15031
15051
15041
15061
15032
15052
15042
15062
15030
15050
15040
15060
15033
15053
15043
15063

HRP Goat anti-Mouse IgG
HRP Goat anti-Rabbit IgG

www.activemotif.com/secondary

35

Name

Absorption

Emissione L/(mol-cm)

Stokes shift

Chromeo™ 642 Goat anti-Mouse IgG

647 nm

666 nm180,000

19 nm

Chromeo™ 642 Goat anti-Rabbit IgG

647 nm

666 nm180,000

19 nm

Rhodamine 6G (GSD) Goat anti-Mouse IgG

508 nm

558 nm116,000

50 nm

Rhodamine 6G (GSD) Goat anti-Rabbit IgG

508 nm

558 nm116,000

50 nm

www.activemotif.com/secondary

36

FormatCatalog No.

1 mg
100 µg
1 mg
100 µg
250 µl35 µl
250 µl
35 µl

15034
15054
15044
15064
15074
15075
15076
15077