The Promoter Activities of Reporter Constructs
HepG2 cells
15.6

74.5

15.4

Relative Light Units (RLU)

75.0

4.0
3.5
3.0
2.5
2.0
1.5

15.2
1.2
1.0
0.8
0.6
0.4

0.5

0.2

0.0

0.0

Po
si.
Neg
a
M .
M
P2IF
N
G
IN
PL S
A
U
PL R
A
T

1.0

Po
si.
Neg
a
M .
M
P2IF
N
G
IN
PL S
A
U
PL R
A
T

Relative Light Units (RLU)

HeLa cells

Hep3B cells
39.0
5.0
4.0
3.0
2.0
1.0
0.0

Posi.: pGL3 Control Vector(SV40 promoter)
Nega.: Empty Vector
MMP2: pGL4-phMMP2 (RDB#7314)
IFNG: pGL4-phIFNG(RDB#7297)
INS:pGL4-phINS (RDB#7387)
PLAUR:pGL4-phPLAUR (RDB#7312)
PLAT:pGL4-phPLAT (RDB#7295)

Posi.
Nega
M .
M
P2IF
N
GIN
PL S
A
U
R
PL
A
T

Relative Light Units (RLU)

40.0

2009, DNA Bank, RIKEN BioResource Center[ GENETYX : Nucleotide Sequence Homology Data ]Date : 2009.07.13
1st Nucleotide Sequence
File Name: Reference Seq.GNUSequence Size: 5481

2nd Nucleotide Sequence
File Name: RDB7297F.fasta

Sequence Size: 765

Unit Size to Compare = 1
Pick up Location[96.447% / 760 bp]
1'= 1

INT/OPT.Score : <462/ 2644 >GGCC TAACTGGCCG GTACCTGAGC TCGCTAGCCT CGAGGAT-AT**** ********** ********** ********** ******* **1" GCCAGAACAT TTCTCTGGCC TAACTGGCCG GTACCTGAGC TCGCTAGCCT CGAGGATAAT
44' TGAGATGCAC CGTAGGCTTA AAATATACAC TGTATTTCNT TTCCTTTTTT CTTTTTTTC********** ********** ********** ******** * ********** *********
61" TGAGATGCAC CGTAGGCTTA AAATATACAC TGTATTTC-T TTCCTTTTTT CTTTTTTTCT
103' TTTTTTTTTT TTTGAGACGG AATCTTACTC NTGTCACCTA GGCTGGAGTG TAGTGGCGCG********** ********** ********** ********* ********** **********
120" TTTTTTTTTT TTTGAGACGG AATCTTACTC -TGTCACCTA GGCTGGAGTG TAGTGGCGCG
163' ATCTCGGCTC ACTGCAACCT CNCACCTTCT GGGTTCAAGC CATTCTCCTG CCTCAGCCTC********** ********** * ******** ********** ********** **********
179" ATCTCGGCTC ACTGCAACCT C-CACCTTCT GGGTTCAAGC CATTCTCCTG CCTCAGCCTC223' CCTAGTAGCT GANGATTACA GGCATACACC ACCATGCCTG GCTAATTTTT TTGTATTTTT********** ** ******* ********** ********** ********** **********
238" CCTAGTAGCT GA-GATTACA GGCATACACC ACCATGCCTG GCTAATTTTT TTGTATTTTT2010 DNA Bank, RIKEN BioResource Center

283' AGTNAGAGAT GGGGTTTCAC CATATTGGCT AGGCTGGTCT CAAACTCCTG ACCTNTGTGA*** ****** ********** ********** ********** ********** **** *****297" AGT-AGAGAT GGGGTTTCAC CATATTGGCT AGGCTGGTCT CAAACTCCTG ACCT-TGTGA
343' TCCACCCGCC TCAACCTCCC AAAGTGCTGG GATTACAGGT GTGAGNCCAC TGCGTCTGGA********** ********** ********** ********** ***** **** **********
355" TCCACCCGCC TCAACCTCCC AAAGTGCTGG GATTACAGGT GTGAG-CCAC TGCGTCTGGA
403' ACTCCCCCTG GGAATATTCT CTACACTGTA TTTCAANGGA TTTAATATGA CAAAAAGAAT********** ********** ********** ****** *** ********** **********414" ACTCCCCCTG GGAATATTCT CTACACTGTA TTTCAA-GGA TTTAATATGA CAAAAAGAAT
463' GTCAAATACC TTATTAACAA TGTAGTANTA TTGATGCATA CTGAAGTACT ATTTGGGATA********** ********** ******* ** ********** ********** **********
473" GTCAAATACC TTATTAACAA TGTAGTA-TA TTGATGCATA CTGAAGTACT ATTTGGGATA
523' TATTGGTTTA AATACAATNA TATTTTAAAA TTATATTTAC CTTTTAAAAA AACTTTTATT********** ******** * ********** ********** ********** **********532" TATTGGTTTA AATACAAT-A TATTTTAAAA TTATATTTAC CTTTTAAAAA AACTTTTATT583' AATGAGGCTN ACTAGATCAT TTAAATTTAC CTGTGTGGCT TGTATTGTAT TTCTACTGGG******************* ********** ********** ********** **********591" AATGAGGCT- ACTAGATCAT TTAAATTTAC CTGTGTGGCT TGTATTGTAT TTCTACTGGG
643' NCAGTGCTGA TCTAGAGCAA TTTGAAACTT GTGGTAGATA -TTTTACTAA CCNAACTCTG********* ******************* ********** ********* **.*******

650" -CAGTGCTGA TCTAGAGCAT TTTGAAACTT GTGGTAGATA TTTTTACTAA CCCAACTCTG
702' A-TGAA-GGA CTT-CCTCA- CCAAA-TTG- TTC-TTTTAA -CCGCA-TTC TTNTCCTTGC* **** *** *** ********** ****** ****** ***** *** **.***

709" ATTGAAGGGA CTTCCCTCAC CCAAATTTGT TTCTTTTTAA CCCGCATTTC TTTTCCC

1st Nucleotide Sequence
File Name: Reference Seq.GNU

Sequence Size

: 5481

2010 DNA Bank, RIKEN BioResource Center2nd Nucleotide Sequence
File Name: RDB7297R.fasta (Complementary)

Sequence Size

: 690

Unit Size to Compare = 1
Pick up Location[97.293% / 702 bp]= 1

INT/OPT.Score : <501/ 2527 >661' AATTTGAAAC -TTGTGGTAG ATATTTTACT AACCNAACTC TGATGAAGGA CTTCCTCACC******* ********* ** .****** **** ***** ******** * **********1"TTGAAAC TTTGTGGTAG AT-NTTTACT AACC-AACTC TGATGAAGAA CTTCCTCACC720' AAATTGTTCT TTTAACCGCA TTCTTNTCCT TGCTTTCTGG TCATTTGCAA GAAAAATTTT********** ********** ***** **** ********** ********** **********
56" AAATTGTTCT TTTAACCGCA TTCTT-TCCT TGCTTTCTGG TCATTTGCAA GAAAAATTTT
780' AAAAGGCTGC CCCTTTNGTA AAGGTTTGAG AGGCCCTAGA ATTTCGTTTT TCACTTGTTC********** ****** *** ********** ********** ********** **********
115" AAAAGGCTGC CCCTTT-GTA AAGGTTTGAG AGGCCCTAGA ATTTCGTTTT TCACTTGTTC
840' CCAACCANCA AGCAAATGAT CAATGTGCTT TGTGAATGAA GAGTCAACAT TTTACCAGNG******* ** ********** ********** ********** ********** ******** *174" CCAACCA-CA AGCAAATGAT CAATGTGCTT TGTGAATGAA GAGTCAACAT TTTACCAG-G
900' GCGAAGTGGG GAGGTACAAA AAAATTTCCA GTCCTTGAAT GGTGTGAAGN TAAAAGTGCC********** ********** ********** ********** *******************232" GCGAAGTGGG GAGGTACAAA AAAATTTCCA GTCCTTGAAT GGTGTGAAG- TAAAAGTGCC
960' TTCAAAGAAT CCCACCAGAA TGGCACAGGT GGGCATAATG NGGTCTGTCT CATCGTCAAA********** ********** ********** ********** ********* **********291" TTCAAAGAAT CCCACCAGAA TGGCACAGGT GGGCATAATG -GGTCTGTCT CATCGTCAAA1020' GGACCCAAGG AGTCTAAAGG AAACTCTAAC TNACAACACC CAAATGCCAC AAAACCTTAG********** ********** ********** * ******** ********** **********
350" GGACCCAAGG AGTCTAAAGG AAACTCTAAC T-ACAACACC CAAATGCCAC AAAACCTTAG

2010 DNA Bank, RIKEN BioResource Center

1080' TTATTAATAC AAACTATCAT CCNCTGCCTA TCTGTCACCA TCTCATCTTA AAAAACTTGT********** ********** ** ******* ********** ********** **********
409" TTATTAATAC AAACTATCAT CC-CTGCCTA TCTGTCACCA TCTCATCTTA AAAAACTTGT1140' GAAAATACGT AATNCCTCAG GAGACTTCAA TTAGGTATAA ATACCAGCAG CCAGAGGAGG********** *** ****** ********** ********** ********** **********
468" GAAAATACGT AAT-CCTCAG GAGACTTCAA TTAGGTATAA ATACCAGCAG CCAGAGGAGG
1200' TGCANGCACA TTGTTCTGAT CATCTGAAGA TCAGCTATTA GAAGAGAAAG ATCAGTTAAG**** ***** ********** ********** ********** ********** **********
527" TGCA-GCACA TTGTTCTGAT CATCTGAAGA TCAGCTATTA GAAGAGAAAG ATCAGTTAAG
1260' TCCTTTGGAC -CTGATCAGC TTATCAAGAT CTGGCCTCGG CGGCCAAGCT TGGCAATCCG********** ********* ********* ********** ********** **********
586" TCCTTTGGAC ACTGATCAGC -TATCAAGAT CTGGCCTCGG CGGCCAAGCT TGGCAATCCG
1319' GTACTGTTGG TAAAGCCACC ATGGAA-GAT GCCAAA-AAC ATTAAGAAGG GCCCAGCGCC********** ********** ****** *** ****** *** *****
645" GTACTGTTGG TAAAGCCACC ATGGAACGAT GCCAAACAAC ATTAAA

2010 DNA Bank, RIKEN BioResource Center