TECHNICALDATASHEETLight Producing Microorganisms

Caution: Research use only. Not for use in diagnostic procedures .

Staphylococcus aureus
Staphylococcus aureus ATCC 49525 (Xen36)
Product No.: 119243
Material Provided: 1 Agar Plate (plate not intended for long-term storage)
Storage Conditions: -80°C in appropriate temperature rated vial ONLY. Refer to Bacterial Growth Guidelines for more detailed
instructions (included in shipment and available at www.perkinelmer.com/microorganisms)

In vitro Characteristics
Genetic Characteristics

Growth Curve

Staphylococcus aureus Xen36 was derived fromthe parental strain Staphylococcus aureus ATCC
49525 (Wright), a clinical isolate from bacteremia
patient. S. aureus Xen 36 possesses a stable copyof the modified Photorhabdus luminescens
luxABCDE operon at a single integration site on anative plasmid.

Log-phase growth can be achieved after 1.5 to 3
hours of subculture in TSB broth at 37ºC withaeration at 200 rpm. An absorbance measurement
at 600 nm (against a TSB blank) of 0.5 is roughly
equivalent to 1.0x108 cfu/ml of S. aureus Xen36.

Growth Characteristics
S. aureus Xen36 grows well in various media
including Brain Heart Infusion (BHI), Trypticase
Soy Broth (TSB), and Luria Bertani (LB) at 37ºC
under ambient aeration. S. aureus Xen36 may also
be grown selectively on medium containing 200μg/ml kanamycin.
Colonial Morphology
On TSA agar plate, S. aureus Xen36 appears as
small (~1.5mm), cream-colored, opaque, smooth,circular colonies.
Virulence Factor
Capsule: Serotype 8.
DNAse: Positive.
NaCl: Tolerant via growth on Mannitol SaltsAgar.
Coagulase: Positive in 4hrs.
mecA: negative

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Biochemical Profile
A biochemical profile was obtained for S. aureus–
Xen36 using the api 20 STAPH system available
from bioMérieux.
Sugar Utilization
D-Glucose
D-Fructose
D-Mannose
MaltoseLactoseTrehalose
D-Mannitol+++++++

Xylitol-Raffinose-Xylose
D-Melibiose-Sucrose+References
1.
2.

Journal of Bacteriology, 2007, 8079-8087, Vol. 189, No. 22
J Immunol. 2007 Nov 1; 179 (9):6160-8

Other Tests
Nitrate Reduction
Alkaline Phosphatase
Voges Proskauer
α-methyl-D-glucosideN-acetyl-glucosamine
Arginine dihydrolase
UreaseProduct Information+++++Warranty
PerkinElmer warrants that cells will be viable
upon shipment from PerkinElmer for a period ofthirty days, provided they have been properly
stored and handled during this period.
Disclaimers
This product is sold for in vivo animal researchuse only and is not for use in any diagnostic
procedures. Excluding purchases by authorized
PerkinElmer distributors, this product is sold foruse by the original purchaser and is not for resale.

Antibiotic Susceptibility
Disk Diffusion Data: Disk diffusion tests wereperformed according to methods outlined in theNCCLS Approved Standard M2-A7.
Kirby-Bauer Disk Diffusion Test
Sensitive to:
Ciprofloxacin 5 μg
Clindamycin 2 μg
Oxacillin 1 μg
Sulfamethoxazole
23.75μg / Trimethoprim
1.25 μg
Tetracycline 30 μg
Chloramphenicol 30 μg
Vancomycin 30 μg
Gentamycin 10 μg

Resistant to:
Kanamycin 30g
Penicillin 10 IU

For more information, please visit our website:www.perkinelmer.com/microorganisms

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