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The ARRIVE guidelines 2.0: author checklist
The ARRIVE Essential 10
These items are the basic minimum to include in a manuscript. Without this information, readers and reviewers
cannot assess the reliability of the findings.
Item
Study design

Recommendation
1For each experiment, provide brief details of study design including:a. The groups being compared, including control groups. If no control group has
been used, the rationale should be stated.
b. The experimental unit (e.g. a single animal, litter, or cage of animals).

Sample size

2a. Specify the exact number of experimental units allocated to each group, and thetotal number in each experiment. Also indicate the total number of animals used.
b. Explain how the sample size was decided. Provide details of any a priori sample
size calculation, if done.

Inclusion andexclusion
criteria

3a. Describe any criteria used for including and excluding animals (or experimentalunits) during the experiment, and data points during the analysis. Specify if thesecriteria were established a priori. If no criteria were set, state this explicitly.
b. For each experimental group, report any animals, experimental units or data points
not included in the analysis and explain why. If there were no exclusions, state so.
c. For each analysis, report the exact value of n in each experimental group.

Randomisation

4a. State whether randomisation was used to allocate experimental units to control
and treatment groups. If done, provide the method used to generate therandomisation sequence.
b. Describe the strategy used to minimise potential confounders such as the orderof treatments and measurements, or animal/cage location. If confounders were
not controlled, state this explicitly.

Blinding

5

Describe who was aware of the group allocation at the different stages of theexperiment (during the allocation, the conduct of the experiment, the outcome
assessment, and the data analysis).

Outcomemeasures6a. Clearly define all outcome measures assessed (e.g. cell death, molecular markers,
or behavioural changes).
b. For hypothesis-testing studies, specify the primary outcome measure, i.e. theoutcome measure that was used to determine the sample size.

Statisticalmethods

7

a. Provide details of the statistical methods used for each analysis, including
software used.
b. Describe any methods used to assess whether the data met the assumptions of
the statistical approach, and what was done if the assumptions were not met.

Experimental
animals8a. Provide species-appropriate details of the animals used, including species, strainand substrain, sex, age or developmental stage, and, if relevant, weight.
b. Provide further relevant information on the provenance of animals, health/immunestatus, genetic modification status, genotype, and any previous procedures.

Experimental
procedures

9For each experimental group, including controls, describe the procedures in enoughdetail to allow others to replicate them, including:a. What was done, how it was done and what was used.
b. When and how often.
c. Where (including detail of any acclimatisation periods).
d. Why (provide rationale for procedures).

Results

10For each experiment conducted, including independent replications, report:
a. Summary/descriptive statistics for each experimental group, with a measure ofvariability where applicable (e.g. mean and SD, or median and range).
b. If applicable, the effect size with a confidence interval.

Section/line
number, or reason
for not reporting