TECHNICALDATASHEETLight Producing Microorganisms

Caution: For Research Use. This product is intended for animal research only and not for use in humans. Not for human or animal therapeutic ordiagnostic use.

Staphylococcus aureus
S. aureus 8325-4 (Xen8.1)
Product No.: 119239
Material Provided: 1 Agar Plate
Storage Conditions: -80°C
In vitro Characteristics
Genetic Characteristics
Staphylococcus aureus-Xen8.1 was derived fromthe parental strain S. aureus 8325-4. S. aureusXen8.1 was engineered through transposition ofTn4001 luxABCDE on plasmid pXen-5. Xen8.1possesses a single stable copy of the modified P.
luminescens lux operon that was inserted in the δtoxin coding region in the RNAIII transcript
downstream of the agr P3 promoter on thebacterial chromosome.

these broth culture conditions, an absorbancemeasurement at 600nm (against a LB blank) of
8
0.6 is roughly equivalent to 5x10 cfu/mL of S.
aureus-Xen8.1 and the relative light intensity is
0.9 photons/sec/cell.

Growth Characteristics
S. aureus-Xen8.1 grows well in Luria Bertani(LB) medium at 37ºC under ambient aeration. S.
aureus-Xen8.1 may also be grown selectively on
LB or BHI agar containing 200μg/mL kanamycin.
Colonial Morphology
On LB agar, S. aureus-Xen8.1 appears as small(~1.5mm), cream-colored, opaque, smooth,circular colonies.
Growth Curve
S.aureus-Xen8.1 displays peak bioluminescenceduring early log-phase growth. Log-phase growth
can be achieved after 1 to 1.5 hours of subculture
in LB broth at 37ºC, shaking at 150-200 rpm. For

119239-Xen8.1-Rev-1

Virulence Factors
Hemolysis: β-hemolysis on TSA + 5% sheep blood
Capsule: literature cites that parental lacks acapsule (CP5 negative), cap5 mutant FEMS
Microbiol Lett 1999 Jan 1;170(1):97-103.

DNAse: Negative
NaCl: Tolerant via growth Mannitol Salts Agar
Coagulase: Positive in 24hrs

MIC and MBC Data
MIC and MBC were determined using the macrodilution methods specified in the NCCLS
Approved Standard M7-A5.

Biochemical Profile
A biochemical profile was obtained for S. aureus–
Xen8.1 using the api 20 STAPH system available
from bioMérieux.
Other Tests

Sugar Utilization
D-Glucose
D-Fructose
D-Mannose
MaltoseLactose
Trehalose
D-Mannitol+++++++Xylitol-Raffinose
Xylose-D-Melibiose+Sucrose+

Nitrate Reduction
Alkaline Phosphatase
Voges Proskauer
α-methyl-D-glucosideΝ-acetyl-glucoside
Arginine dihydrolase
Urease+++++Antibiotic

MIC (µg/mL)

MBC (µg/mL)

Ceftriaxone

>32

NA

Ciprofloxacin

>8.0

NA

Erythromycin

1.0

1.0

Gentamicin

2.0-4.0

4.0-8.0

Kanamycin>125

NA

Penicillin G

0.013-0.063

0.063-0.125

Tetracycline

0.250.5(trailing endpoint)

References
1. Photochem. Photobiol. Sci., 2004, 3, 451 – 458
2. Photochem. Photobiol. Sci., 2005, 4, 503 – 509
3. Biomaterials, Volume 27, Issue 22, August 2006, Pages 4157-4164
4. Wound repair and regeneration, Volume 16 Issue 3, Pages 425 - 431

Product Information
Warranty

Antibiotic Susceptibility
Disk Diffusion Data Disk diffusion tests wereperformed according to methods outlined in theNCCLS Approved Standard M2-A7.
Kirby-Bauer Disk Diffusion Test
Sensitive to:Carbenicillin 100
Gentamicin 20
Penicillin G 10U
Vancomycin 30

NCCLS Macrodilution MIC/MBC

Resistant to:
Kanamycin 30PerkinElmer warrants that cells will be viable
upon shipment from PerkinElmer for a periodof thirty days, provided they have been
properly stored and handled during this period.
Disclaimers
This product is sold for in vivo animal researchuse only and is not for use in any diagnostic
procedures. Excluding purchases by authorized
PerkinElmer distributors, this product is sold foruse by the original purchaser and is not forresale.

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119239-Xen8.1-Rev-1