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(NADPH) ïĂČòđ(ATP) r¦$P

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1.9Ã¦àdn°¦hÛ
Mn4CaO5óċùûđÛÊé ìÔØĕĒ

Umena, Kawakami, Shen, Kamiya, Nature , 2011�����������������
��������������r¦ßÌÒéMn#:à^5!
2H20 ! 4H+ + 4 eĚ + O2
4Mn(III2, IV2)

Mn4CaO5-cluster-clusterKok cycle model
H+
e-
S1

e-

4Mn
( III3, IV)? S0

4Mn
IV3)
S2 (III, IV3)
H2O

H+

H2O

O2

e-
H+

S4

H+ e-
B. Kok, et al, (1970), ÅV. K. Yachandra, et al., (1993)
H.Dau, et al., (2007)(Mn#:à^áĘ
ÝàMn#:Î´!ÓêéĘ

S3 4Mn(IV4)?

IV?
III?Mnor IV?
Mn
Mn

III?
Mn
or IV?Mn
Mn

Mn

III?
or IV?

Mn III? or IV?7lx)#:ÜdQà.Á"!Å x ) # :  ~ R ß Ê é 4 l à M n ¸  n °Fluorescence X-ray

X-ray
X-ray

X-ray absorption spectrum frommixed valence Mn metal complexxSÔØùćóÿČ
Mn metals"!Å  d n ° à # :   J Î Ë   «  d•!

,r55% ! :à[çÐ•!

CrikshankàDPIÍç¥åç
êé#:º­¾àªH

Mn4CaO5-óċùûđ

0.16Ã (1.9Ã, 3WU2)

^ßPÕØX-%ÜI]
Ĉďðď´!}àX-%ùćóÿČ

normalized F/ Io

Mn (IV)O2
Mn (II)

1.887

Mn(III)
Mn (III) 2O3
Mn (II) O
Mn (IV)^ė8^ėB-%^ėB-%^ė8I]ėBI]ė8

1.89

1.893
wavelength (/Ã)

1.896

1.899

s¹ßæéI]H¿:?Jà±Ë
Mnà-%s¹(1.892ÃĔßÌÒéÆI]ÀH¿:?JĈüĆàLJHÎ^à±Ëì$cÔÚËéÇ#) $!#"%!  % $%)&MnÜCaàę-%ùćóÿČàoK1

(·Dß@PÖéI]ÀH¿:?JĈüĆàs¹;

' %I]À¿:?JĈüĆàs¹;
s¹ 0.900Ã

s¹ 1.892Ã$   %#+"!"#60

0.900A

1.892A

Mn2

Mn3

1.896A

50403020

100Mn1

Mn4

Caà.Á{2Þ6MàPSIIdX|Aßæé·D#:à³
>MÛ8ÏÓÎ>ÛáÞË
PSIId����������������������������

Number of
Diffraction image

K--%

•!Å¹s¹XáX-% jÎ8ÏË•!Ådà` Đ Û-% jÎ5ëé•!Å/Uþđûàdºà*3QÎ •!ÅX|AßæÙÚMnÎ³Óêé•!ÅùćóÿČà-% à5!
•!ÅMnàX-%ßæé jÎaÖéw6čđ÷đßæèdàkGw6čđ÷đG¡èÓêéPSIId1/®Ó×Úk~ßG

2

3

54āõď¢w6čđ÷đNSL-193L
s¹ė193.4nm
Beam size: FWHM 4um
Pulse power: 1µ J/pls
Pulse width: <1.3ns óĎùöîú:50 µm

Kitano H. et al., Jpn. J. Appl. Phys. (2005)È{É£^à*MÞkdÍçàþđû%½,NàkGÔØd•!Å|ATà"äēĔÎ>!! ę-%à`;ÎCÞË!! d/ºÛ*«Þ/Uy>•!Å=vÔØę|AÎ&!! ę¶(dose)à¥åèÎp#!$$$*&#) $*( &£^TÛ/ULJþđûà%½

!! ĄüóôċďĀàăîúÎCÞËAdvantage of cylinder shaped crystal 1 "(#$ "!$#$$#$#'&$ Åresolution (Ã)

No Process
Cylinder shape
50-2.5 (2.54-2.50)

mosaic range (°)

0.39-0.43
1,075,057
0.14 (0.85)
15.5 (1.5)
97.0 (90.3)

No of reflection
R-merge<I>/<! (I)>completeness (%)

0.34-0.41
1,100,974
0.09 (0.48)
25.2 (2.5)
98.6 (98.3)

Scale factor

&  !#!$$ ) #"#!$$

0

100 200 300 400 500

Frame Number

R-merge$ "&#"

12108
64
20*

 "#) #$"! "#!$$
"&#"

0.3
0.25
0.2
0.15
0.1
0.05
0

&  !#!$$ ) #"#!$$

0

100 200 300 400 500

Frame number

Advantage of cylinder shaped crystal 2 #!Multi-crystal data collection

Rmerge0.30.25cylinder0.2

none0.150.1
0.05

150 °(30° x 5 crystals)


00306090
oscilation range (/degree)

120#!Accurate X-ray Dose estimation+ &) #$"#!$$$*&#) $*( &150

Advantage of cylinder shaped crystal 3 
0

0

45
90

45
90

XAFS spectra at different crystal orientations
0.03

0.03
0.025

0deg_none
45deg_none
90deg_none

0.025

0.02

0.02

0.015

0.015

0.01

0.01

0.005

0.005

00-0.005
6530 6535 6540 6545 6550 6555 6560 6565 65700deg_clynd
45deg_cylnd
90deg_cylnd-0.0056530 6535 6540 6545 6550 6555 6560 6565 6570

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