ܿ੅঩ၳTR08AάȜ·؊൞଻͈ၻ̞ไဥŐŅŔ΃ρθࣞޭ଻̥ͣ೩ޭ଻‫ࣣا‬໤́͘ΨρϋΑ͈̹৾ͦไဥ߿ŐŅŔࡥ೰௖Άॸ֊൲௖̤̫ͥͅ‫ࣣا଻ܖ؂‬໤͈స‫̹̱ק‬؊൞଻࿹̹ͦၛఘ஖఼଻ġİġ႒‫׻‬ఘ͈໦ၗ଻ෝঀ̞ੳ਀͈ၻ̞ࣞၑა౲ˏЂ̉ၥঊ΃ρθ஠ఉࢢ଻‫ેݩ‬Ώς΃ġİġˏЂ̉ၥঊİġĲĳůŮळࢢİġńĲĹࡥ೰௖ġİġűŉġĳĮĲıġİġచય໦ঊၾġĽĲŎŅŢġİġŖŔőġōĲġ૧‫ٳ‬อ ͈ ນ ࿂ ੜ ၑ ܿ ੅Polar End-Capping Technology

1OH

32

OCH2CHCH2NHCH

1

2CH3CH3

propranolol

CHOCH2CH2 N

CH3CH3diphenhydramine
3O
CHCH2CH2NCH3
CH3

doxepine

CONVENTIONAL C18
CONVENTIONAL C18
0.1% HCOOH in acetonitrile/waterΧͼήςΛΡΏς΃ͬ‫͚܄‬ਲြ͈Ώς΃ࠏŐŅŔ΃ρθ͉́Ȅ΂·ΗΟΏσ‫͈ܖ‬൵වࢃȄΏς΃ນ࿂ͅॼͥȶॼంΏρΦȜσ‫ܖ‬ȷͬࡘઁ̵̯̹͈ͥ͛ȶ΀ϋΡ΅λΛάϋΈੜၑȷ̦ຈါ̳́ȃȁ̭͉ͦͅιΙσΏςσ‫ࣣا‬໤ͥ͢ͅນ࿂ੜၑ༹༷̦ഥൡഎͅဥ̞̞̳ͣͦ̀͘ȃȁ̱̥̱‫ݙ‬௖໦ၗ͈۷ത̥̳ͣͥ͂ȄΏς΃ນ࿂͉ͅŐŅŔς΄ϋΡဇြ͈மକ‫͂ܖ‬Ώς΃ນ࿂ષ͈΀ϋΡ΅λΛάϋΈဇြ͈மକ‫̠̞͂ܖ‬ඵਅ႒͈։̈́ͥமକ‫̦ܖ‬ంह̳̭ͥ͂̈́ͤͅȄமକഎ௖ࡽैဥ͈؊൞̦ະ޳֚̈́ͥࠫͅ‫ض‬ȄάȜ·ࠁે͞ၑა౲ତͅ՛‫ͬޣג‬ဓ̢ͥાࣣ̦̜̳ͤ͘ȃΠςιΙσΏςσ‫ܖ‬ĩŕŎŔĪ͈̈́̓ࡣങഎ̈́ιΙσΏςσ‫΀ͥ͢ͅܖ‬ϋΡ΅λΛάϋΈ͉̩́̈́Ȅ‫ڟ‬૧എ̈́ޭ଻‫ܖ‬Ȫ΀ȜΞσȄକॸ‫ܖ‬ȫͥ͢ͅ΀ϋΡ΅λΛάϋΈ༹ͬ‫ٳ‬อ̱ȄńŢťŦůŻŢġńřĮńĲĹͅनဥ̱̱̹͘ȃġဣৗ͈மକ‫·΂̤͉͜ͅܖ‬ΗΟΏσ‫ܖ‬ĩŐŅŔĪ͂௖ࡽैဥ̱Ȅ૶କ‫΀ܖ଻ޭ͉ܖ‬ϋΡ΅λΛάϋΈࢹ௮͂୓ഩഎ௖ࡽैဥ̱̳͘ȃ̷͈ࠫ‫ض‬Ȅமକ଻͈̞ࣞ‫ࣣا଻ܖ؂ޑ‬໤͈άȜ·ࠁે͞ၑა౲ତ͈٨஝̦‫ܢ‬ఞ̯̳ͦ͘ȃȁ̹͘Ȅࡥ೰௖ນ࿂ષ͈மକ‫ܖ‬İ૶କ‫ܖ‬͈໦ື̦ߗ‫ۼ‬എͅޫह‫̭̳̀̽͂ͥ͢ͅا‬ဣৗ͈ၛఘࢹ௮ͬ෇ে̳ͥ࢘‫̦ض‬௩̱Ȅਲြ΃ρθ͉͂։̈́ͥ໦ၗඅ଻̦ංͣͦͥ‫خ‬ෝ଻̦̜̳ͤ͘ȃ૧ܰ̈́΀ϋΡ΅λΛάϋΈ༹ͥ͢ͅńŢťŦůŻŢġńřĮńĲĹ̽̀͢ͅȄไဥ଻͈̞ࣞ‫ݙ‬௖໦ၗ͈ଲ‫̳̦ͤ͘ࢩ̦ٮ‬ȃ
Imtakt USA (info@imtaktusa.com)
North America

Imtakt Corp./JAPAN (info@imtakt.com)
Other Countries

TR08AJ-RJ25స‫̹̱ק‬άȜ·ࠁેȆ໦ঊ෇েෝȆࣞၑა౲ਲြ͉͂։ْ̈́ͥ‫ܢ‬എ̈́ޭ଻‫΀ܖ‬ϋΡ΅λΛάϋΈ༹ͬ‫ٳ‬อ̱ȄńŢťŦůŻŢġńřĮńĲĹͅनဥ̱̱̹͘ȃࡣങഎ̈́ιΙσΏςσ‫΀ͥ͢ͅܖ‬ϋΡ΅λΛάϋΈࢹ௮͉̞̭̥́̈́͂ͣȄਲြ΃ρθͅ๤͓̀ȄΏρΦȜσ̦ࡔ֦͂̈́ͥάȜ·؊൞଻̦٨஝̳ͥ͂͂͜ͅȄ޳֚̈́ນ࿂மକ଻̦ၑა౲ତ͈࢜ષ͜ͅ‫ܙ‬ဓ̱̳͘ȃ‫ུܖ‬എ௖ࡽैဥ
Electrostatic Interaction

Hydrophobicity

Steric Selectivity

Cadenza CW-C18

BRAND-Z

BRAND-X

Cadenza CD-C18

Cadenza CL-C18

Cadenza CD-C18

Cadenza CL-C18

Cadenza CW-C18

Cadenza CW-C18

Cadenza CD-C18

BRAND-L

BRAND-B

BRAND-L

BRAND-Z

BRAND-L

BRAND-Z

BRAND-B

Unison UK-C18

Unison UK-C18
BRAND-X

Unison UK-C18
BRAND-X

Cadenza CL-C18
0.0

0.1

0.2

0.3

0.4

0.0

0.5

water / methanol = 70 / 30
H3CO
NON

ńŢťŦůŻŢġ ńřĮńĲĹ͈ξΣȜ·̈́ޭ଻‫΀ܖ‬ϋΡ΅λΛάϋΈࢹ௮͉Ȅ‫ུܖ‬എ̈́༗঵Ȇ໦ၗඅ଻ͅਲြ͉͂։̈́ͥඅ଻ͬဓ̢̞̳̀͘ȃഐഽ̈́ŐŅŔ൵වၾͤ͢ͅȄமକഎ௖ࡽैဥ͉ŖůŪŴŰůġ ŖŌĮńĲĹ͂႒য̱̞̳̀͘ȃȁࣞޭ଻໤ৗ͈༗঵ෝͬা̳୓ഩഎ௖ࡽैဥ͉ȄńŢťŦůŻŢġ ńŅĮńĲĹͤ͢͜ઁ̱ఱ̧̧̩͉̹̳ͣ͘ȃȁၛఘࢹ௮͈։̈́ͥ႒‫׻‬ఘ͈໦ၗͬা̳ၛఘ஖఼଻͉Ȅਲြ΃ρθͅ๤͓̀ఱ̧̈́౵̞̳͂̈́̽̀͘ȃȁ̭ͦͤ͢ͅࢹ௮։଻ఘ͈̈́̓໦ঊ෇ে͈࢜ષ̦‫ܢ‬ఞ̯̳ͦ͘ȃ

CH30.5

BRAND-B1.01.52.00.0

water / methanol = 20 / 80

OHCH2CH2CH2CH2CH30.5

1.0

1.52.0water / methanol = 20 / 80

CH2CH2CH2CH3NN
CH3caffeine

phenolamylbenzene

butylbenzeneၛఘ஖఼଻triphenylene o-terphenyl։଻ఘȆ႒‫׻‬ఘ໦ၗ(2/1) =1.4uracil

ͺσ΅σङ͈պ౾։଻ఘ̜́ͥΩραϋ։଻ఘ͉மକഎ໦ঊ෇ে͈ങ߿എম႕̳́ȃńŢťŦůŻŢġ ńřĮńĲĹġ ͈։଻ఘ໦ၗ͉Ȅ൚২ਲြ΃ρθ͂൳൝ոષ͈଻ෝͬอ‫̳̱͘ܞ‬ȃ

BRAND-XB C18Rs(4/3) = 1.9

1 methylparaben
2 ethylparaben
3 isopropylparaben
4 propylparaben
5 isobutylparaben
6 butylparaben

BRAND-XB C18 (3.5 µm)
12o-terphenyl

triphenylene

5 6150 x 3 mm
acetonitrile / water / acetic acid = 40 / 60 / 0.1
0.4 mL/min, 37°C, 260nm

150 x 3 mm
MeOH / water = 80 / 20
0.4 mL/min, 37°C
260nm
11234123456

2Rs(4/3) = 2.6(3µm)(2/1) = 2.2

13324456Rs(4/3) = 2.3
34Rs(4/3) = 2.4(2/1) =1.4

13205211O

201020 min

N CCH3N-acetyl-4benzoquinone imine

2 HO
115O1(2/1) = 1.6

Cadenza CD-C18410HN CCH3Oacetaminophen

220 min2978ńŢťŦůŻŢġ ńřĮńĲĹ͉‫ࣣا‬໤͈ၛఘࢹ௮͈ে༆ͅ࿹̞̳ͦ̀͘ȃġ ໹࿂എ̈́ġ ŵųŪűũŦůźŭŦůŦġ ͂ၛఘഎ̈́ŵŦųűũŦůźŭġ ͈໦ၗ଻ෝ͉Ȅਲြ΃ρθͤ͢͜࿹̞̳ͦ̀͘ȃġ ̭͉ͦͅŐŅŔς΄ϋΡո‫ٸ‬ນ࿂ޭ଻ࢹ௮̦‫۾‬߸̱̞̀ͥ͂ࣉ̢̳ͣͦ͘ȃࢹ௮։଻ఘ͞႒যࢹ௮‫ࣣا‬໤͈໦ၗ٨஝ͅńŢťŦůŻŢġńřĮńĲĹ͈ഐဥ̦‫ܢ‬ఞ̯̳ͦ͘ȃłŤŦŵŢŮŪůŰűũŦůġ̷͈͂య৫໤ͅ ‫ ̀ ̱ ۾‬ń Ţ ť Ŧ ů Ż Ţ ń ř Į ń Ĳ Ĺ ͉ȄŖůŪŴŰůġŖŌĮńĲĹġ͉͂ဣ੄ਜ਼੬̦‫ݙ‬ഢ̳ͥࠫ‫̹̱ͤ̈́͂͘ض‬ȃȁ̭͉ͦࡥ೰௖ນ࿂͈૶କ଻ࢹ௮̦ၰ৪́։̭̦̈́ͥ͂ၑဇ͂ࣉ̢̳ͣͦ͘ȃਲြ߿΃ρθ͉́ࣾඳ̈́႒‫׻‬ఘ͈໦ၗͅȄńŢťŦůŻŢġ ńřĮńĲĹ͈ခ࢘଻̦‫ܢ‬ఞ̯̳ͦ͘ȃ1
150 x 3 mm
water / ACN / HCOOH= 95 /5 /0.1
0.4 mL/min, 37°C, 260nm

051015 min9822ॸ଻֊൲௖̤̫ͥͅ‫ࣣا଻ܖ؂‬໤͈࿹̹ͦάȜ·ࠁે‫ࣣا଻ܖ؂‬໤ͬ໦ଢ଼̳ͥા๊ࣣ͉֚ͅഎͅॸ଻֊൲௖̦ဥ̞̳ͣͦ͘ȃȁ̧̭͈ٜ͂ၗ̱̹‫ࣣا଻ܖ؂‬໤͂ॼంΏρΦȜσ͈ະ޳֚̈́௖ࡽैဥͤ͢ͅȄάȜ·͈ΞȜςϋΈ̦୆̲ͥાࣣ̦̜̳ͤ͘ȃȁޭ଻‫́ܖ‬ໞ̹ͩͦńŢťŦůŻŢġ ńřĮńĲĹ͈޳֚̈́ࡥ೰௖ນ࿂͉Ȅ‫ࣣا଻ܖ؂‬໤ͅచ̱̀޳֚̈́؊൞ͤ͢ͅΏλȜί̈́άȜ·ࠁેͬা̱̳͘ȃ‫ࣣا଻ܖ؂‬໤ȪΆॸ֊൲௖ȫO

1

CHCH2CH2N

2doxepine

122

1CH3NCH33N
CH2CH2 CH2Nimipramine

C1(3µm)

CH2CH2 N

Cl

CH3chlorpheniramine

CH3CH3N

CHCH2CH2Namitriptyline

3 44CH32CH3H3C O

NCH2CHCH2N
CH3dextromethorphan

CH3CH3trimipramine

O103

BRAND-XB C18510

COCH2CH2CH3HO(3µm)

150 x 3 mm
A: water / HCOOH = 100 / 0.1
B: ACN / HCOOH = 100 / 0.1
CX-C18 (20%B)
Brand-XB (38%B)
0.4 mL/min , 37°C, 260nm

43CH3H

32CH3propylparaben
A: water / formic acid = 100 / 0.1
B: acetonitrile / formic acid = 100 / 0.1
5-95%B (0-15min)
0.4mL/min, 37°C, 280nm

BRAND-XB C18(3.5µm)

15min051015 min

972984ͼ΂ϋ‫ޑ‬ഽ͈೩̞Άॸ֊൲௖́ΞȜςϋΈ̱̳̞͞२۪ࠏࢯ̠̾࿪ͅ‫̱۾‬ȄńŢťŦůŻŢġńřĮńĲĹ͉స‫̹̱ק‬άȜ
·ࠁેͬা̱̳͘ȃ໓৯࿪͞೉‫ٹ‬࿪଼͈໦͉ͅ‫ࣣا଻ܖ؂‬໤̦ဥ̞̞̳ͣͦ̀͘ȃȁńŢťŦůŻŢġ ńřĮńĲĹ͉Άॸ֊൲௖́͜࿹̹ͦ໦ၗ͂άȜ·ࠁેͬা̱̳͘ȃtrans-doxepin

31

cyclohexylamine[M+H]+ m/z 100.1

NH242O

21

NH21-hexylamine[M+H]+ m/z 102.1

1-octylamine[M+H]+ m/z 130.1

3

CHCH2CH2N

1-octadecylamine[M+H]+ m/z 270.3

4

Conventional C18150 x 3 mm
A: water / formic acid = 100 / 0.1
B: acetonitrile / formic acid = 100 / 0.1
0-100%B (0-7min), 100%B (7-10min)
0.4 mL/min (7-8MPa), 37°C
2µL (10ug/mL, 0.1%HCOOH in 60%ACN)
ESI (positive)

10250 x 3 mm
water / acetonitrile / formic acid= 88 /12 / 0.1
0.4 mL/min, 37°C
20µL (100ng/mL, 0.1%HCOOH)
ESI, positive

NH2

324

6

[M+H]+ m/z 280.1

CH3doxepin

NH2

4
2

CH308 min1020

cis-doxepin

30 min989980ńŢťŦůŻŢġ ńřĮńĲĹ͉ࣞޭ଻໤ৗ̺̫̩́̈́Ȅডཛ௼ͺηϋ͈̠͢ͅமକ଻͈̞ࣞ‫ࣣا‬໤ͅచ̱̀͜ၻࢡ̈́؊൞ͬা̱̳͘ȃŅŰŹŦűŪůġ͉ͅŤŪŴĭġŵųŢůŴġ։଻ఘ̦ంह̱̳̦͘Ȅਲြ΃ρθ͉́ΞȜςϋΈ̦ࡔ֦͂̈́ͤȄΆॸ֊൲௖͉́ōńĮŎŔ໦ଢ଼̦ࣾඳ̱̹́ȃńŢťŦůŻŢġ ńřĮńĲĹ͉ȄΆॸ֊൲௖́͜ၻࢡ̈́։଻ఘ໦ၗͬ‫خ‬ෝ̱̳͂͘ȃ4.0(x10e6)

Powdered
Coptis Rhizome 10mg
1% formic acid 1mL

3.0O

berberine
2.7ng[M]+ m/z 336.1

mixing(sonification, 10min)

N+OO

centrifugation(12 krpm, 10 min)berberine

2.0Supernatant

150 x 3 mm
A: water / formic acid = 100 / 0.1
B: acetonitrile / formic acid = 100 / 0.1
0-90%B (0-10 min)
0.4 mL/min (9 MPa), 37°C, 5 µL
ESI (positive)

1000-fold dilution
with 0.1% formic acid

1.0O

Injection Sample
0.0012345678910 min
9933΂;τϋྎಎ͈৽଼໦ͺσ΃υͼΡ̜́ͥţŦųţŦųŪůŦ͜Άॸ֊൲௖́۰ౙͅōńĮŎŔ໦ଢ଼̦‫خ‬ෝ̳́ȃ̯̰̈́͘͘‫ࣣا‬໤͈͒ഐ؊଻ńŢťŦůŻŢġ ńřĮńĲĹ͉‫ࣣا଻ܖ؂‬໤̴͈͙̈́ͣȄॸ଻‫ࣣا‬໤͞ಎ଻‫ࣣا‬໤̈́̓Ȅࢩํս͈໤ৗ໦ၗͅഐ؊̳ͥ଻ෝ̦̜̳ͤ͘ȃġ̹͘ޭ଻‫΀ܖ‬ϋΡ΅λΛάϋΈͤ͢ͅȄਲြ΃ρθ͉͂։̈́ͥ໦ၗ஖఼଻̦‫ܢ‬ఞ̯̳ͦ͘ȃॸ଻‫ࣣا‬໤͈͒ഐဥ
150 x 3 mm
A: 50mM ammonium formate
B: acetonitrile
25-95%B (0-10min)
0.4mL/min, 37°C, ELSD

1O OS
ONa

1

150 x 3 mm
A: 50mM ammonium formate
B: acetonitrile
40-85%B (0-10min)
0.4 mL/min, 37°C
ESI (negative) 100pg

sodium heptylsulfonate
O OS
ONa

22sodium octylsulfonate

313

12

F

F FF FF F O

F FF FF FF FO OSO
ONaNa+F FF F O O
SOHF FF FPFBS
perfluorobutanesulfonic acid

23FOHPFOA
perfluorooctanoic acidsodium dodecylsulfate[M-H]- m/z 298.9

3[M-H]- m/z 412.9

PFOS
perfluorooctanesulfonic acid

[M-H]- m/z 498.9
024

6F FF FF FF F O O
SOHFF FF FF FF F

810 min
990

0510

őŇŐŔ͞őŇŐł͈̈́̓ခ‫ܥ‬έΛள‫ࣣا‬໤͉ȄέΛளਏড͞έΛளࠏນ࿂ੜၑस
ͅဇြ̳۪ͥ‫۾ޏ‬Ⴒ‫ࣣا‬໤̳́ȃńŢťŦůŻŢġ ńřĮńĲĹ͉ಎ଻űŉ֊൲௖͂ņŔŊĮŏŦŨŢŵŪŷŦκȜΡͤ͢ͅȄࣞۜഽͅōńĮŎŔ໦ଢ଼̳̭̦ͥ͂‫خ‬ෝ̳́ȃ

15 min991

ńŢťŦůŻŢġ ńřĮńĲĹ͉மକ‫΂ͼ͂ܖ‬ϋ଻૶କ‫̦ܖ‬ޫह̳ͥ‫ٮ‬࿂‫଻ڰ‬स͈໦ၗ͜ͅ਱໦ͅഐဥ̧́ͥไဥ଻̦̜̳ͤ͘ȃ๤‫ڛ‬എமକ଻͈̞ࣞͺσ΅σၤॸ͞ͺσ΅σΑσγϋॸͅచ̱̀͜ȄńŢťŦůŻŢġ ńřĮńĲĹ͉࿹̹ͦ؊൞ͬা̱̳͘ȃβίΙΡ໦ၗ஖఼଻
51. Angiotensin III (Human)[M+2H]2+ m/z 466.2
2. [Val5]- Angiotensin I (Bovine)[M+3H]3+ m/z 428.2

1̯̰̈́͘͘‫ࣣا‬໤͈͒ഐ؊଻

32

3. Angiotensin II (Human)[M+2H]2+ m/z 523.7

45βίΙΡ͈ōńĮŎŔ໦ଢ଼͉́ȄΆॸ֊൲௖๊̦֚ͅঀဥ̯̳ͦ͘ȃńŢťŦůŻŢġ ńřĮńĲĹ͉Άॸ֊൲௖̤̫ͥͅ‫ا଻ܖ؂‬ࣣ໤͈؊൞ͅ࿹̞̳̦ͦ̀͘Ȅ൳အͅ΃Ι΂ϋ଻͈βίΙΡͅచ̱̀͜ၻࢡ̈́άȜ·ࠁે͂࿹̹ͦ໦ঊ෇েෝͬอ‫̳̱͘ܞ‬ȃΆॸ֊൲௖ͥ͢ͅōńĮŎŔ໦ଢ଼ͅ‫̱̀۾‬ńŢťŦůŻŢġ ńřĮńĲĹ͉Ȅ‫ࣣا଻ܖ؂‬໤͞βίΙΡ̺̫̩́̈́Ȅ̞̞ͧͧ̈́‫ࣣا‬໤͜ͅഐဥ̯̭̦ͦͥ͂‫ܢ‬ఞ̯̳ͦ͘ȃୋ຦ૂ༭

4. Angiotensin I (Human)[M+3H]3+ m/z 432.9

1

5. Angiotensin IV (Human)[M+2H]2+ m/z 388.2
3

BRAND-XB C180224468 min

150 x 3 mm
A: water / formic acid = 100 / 0.1
B: acetonitrile / formic acid = 100 / 0.1
CX-C18 (5-30%B in 15min)
Brand-XB (15-40%B in 15min)
0.4 mL/min, 37°C, ESI (positive)988

৽̈́ॽအȇȁၥঊࠂȇĴЂ̉ġĶЂ̉ȄġळࢢࠂȇĲĳ̊̉Ȅġࡥ೰௖ȇ΂·ΗΟΏσ‫ܖ‬Ȅޭ଻‫΀ܖ‬ϋΡ΅λΛάϋΈȄġఛգȇĳĶŎőŢġĩŉŕĻĶıŎőŢĭġŖőĻĲııŎőŢĪĭġŖŔőġōĲġ

΃ρθྴ

(3µm)
(5µm)

΃ρθඤࠂ΃ρθಿ

1mm, 1.5mm, 2mm, 3mm
4.6mm, 6mm, 10mm
20mm(5µm), 28mm(5µm)

10mm, 20mm, 30mm, 50mm
75mm, 100mm, 150mm
250mm, 500mm(3µm)΄ȜΡ΃ρθ΄ȜΡγσΘȜ΃ȜΠςΛΐ΃ρθȶη·υİ΢Φ΃ρθȷġȶιΗσέςȜ΃ρθȷ̞͉̤̾̀ͅ࿚̵̞ࣣ̩̺̯̞ͩȃ