TECHNICALDATASHEETLight Producing Microorganisms

Caution: For Research Use. This product is intended for animal research only and not for use in humans. Not for human or animal therapeutic ordiagnostic use.

Escherichia coli
E. coli WS2572 (Xen14)
Product No.: 119223
Material Provided: 1 Agar Plate
Storage Conditions: -80°C
In vitro Characteristics
Genetic Characteristics

Growth Curve

Escherichia coli–Xen14 was derived from theparental strain Escherichia coli WS2572, aclinical isolate from Weihenstephan Culture
Collection. E. coli-Xen14 possesses a stable copyof the Photorhabdus luminescens lux operon on
the bacterial chromosome.

Log-phase growth can be achieved after 1 to 1.5
hours of subculture in LB broth at 37ºC, shaking
at 150-200 rpm. For the above broth culture
conditions, an absorbance measurement at 600nm(against a LB blank) of 1.0 is roughly equivalent
to 1.1x109 cfu/mL of Escherichia coli–Xen14 andthe relative light intensity is 1.01 photons/sec/cell.

Growth Characteristics
E. coli-Xen14 grows well in Luria Bertani (LB)
medium at 37ºC under ambient aeration. E. coliXen14 may also be grown selectively on LB agar
containing 30μg/mL Kanamycin.
Colonial Morphology
On LB plates, the E. coli-Xen14 appears as
medium (~2mm), translucent, conical colonies
after 24 hours incubation at 37°C.

119223-Xen14-Rev-1

Biochemical Profile
A biochemical profile was obtained for E. coliXen14 using the api 20E system available frombioMérieux.
Sugar Utilization-+-+

Indole Production+

Voges Proskauer
Gelatinase-Oxidase-+++++

Amygdalin
Arabinose

1.
2.

Infection & Immunity, Aug. 2005, 4512-4521
Antimicrobial Agents & Chemotherapy, Jan. 2001, p129-137

Other Tests
β-galactosidase
Arginine Dihydrolase
Lysine Decarboxylase
Ornithine Decarboxylase
Citrate Utilization
H2S ProductionUrease
Tryptophan Deaminase

GlucoseMannitolInositol
Sorbitol
Rhamnose
SucroseMelibose

References

Product Information
Warranty
PerkinElmer warrants that cells will be viable
upon shipment from PerkinElmer for a period ofthirty days, provided they have been properly
stored and handled during this period.
Disclaimers
This product is sold for in vivo animal researchuse only and is not for use in any diagnostic
procedures. Excluding purchases by authorized
PerkinElmer distributors, this product is sold foruse by the original purchaser and is not for resale.

Antibiotic Susceptibility
Disk Diffusion Data: Disk diffusion tests wereperformed according to methods outlined in theNCCLS Approved Standard M2-A7.
Kirby-Bauer Disk Diffusion Test
Sensitive to:
Chloramphenicol 30
Tetracycline 30
Trimethoprim/
Sulfamethoxazole
Gentamicin 10

Resistant to:Carbenicillin 100
Kanamycin 30
Streptomycin 10
Cephalothin 30
Ampicillin 10

For more information, please visit our website: ww.perkinelmer.com/microorganisms.
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119223-Xen14-Rev-1