MPA Series MPA-10/20/200/1200/10000
Single Channel Electronic Pipettes
Supplementary Instruction Manual
The MPA Series is equipped with the following operation modes
when there is a requirement for higher order dispensing operation.

1.

Introduction

This document provides supplementary information for the operation
modes listed below which have been added to the MPA Series ofelectronic pipettes.
1 ) Dispensing and mixing mode (AUTO＋MIX)
By combining Standard mode (AUTO) and Mixing mode (MIX),
the pipette can continue to mix the substance after the standard
dispensing operation has been completed.
It is possible to individually set the dispensing amount, numberof mixes and mixing volume.
2 ) Sequential aspirating mode (SA) （SA: Sequential Aspirating）
Different types of liquid can each be aspirated at individually set
volumes, then dispensed all together.
3 ) Sequential dispensing mode (Sd) （Sd: Sequential Dispensing）
Aspirated liquid can be sequentially dispensed at individually set
volumes.

1WMPD4003037A

２. Explanation of each operation mode
2-1.

Dispensing and mixing mode (AUTO＋MIX)

1 ) Selection and setting method for Dispensing and mixing modeThe example below uses the MPA-200 and mixes 200μL(100μL of liquid dispensed into 100μL prepared earlier) 10times.
（Example of initial display）
Setting example starts below[ 1 ] Press theBack key.

[ 2 ] Select “AUTO” using thekey.

Up/Down[ 3 ] In “AUTO” mode hold theEnter key
down for around 3 seconds.

[ 4 ] Stop pressing theEnter key.

[ 5 ] Set the dispensing amount using theUp/Down key.

[ 6 ] The dispensing amount is determinedby pressing theEnter key.

2

[ 7 ] Set the number of mixes using theUp/Down key.
[ 8 ] The number of mixes is determined bypressing theEnter key.

[ 9 ] Set the mixing volume using theUp/Down key.
[ 10 ] The mixing volume is determined bypressing theEnter key.

2 ) Operation method for Dispensing and mixing mode
[ 1 ] After placing the end of the tip into theliquid to be dispensed (A), press theOperation key on the body of thepipette to aspirate the liquid.

Liquid（A）[ 2 ] After putting the end of the tip into thevessel containing liquid for mixing (B),press theOperation key to dispense
the aspirated liquid (A）Liquid to be dispensed（A）
Liquid（B）

3

[ 3 ] Put the end of the tip into the combined
liquid for mixing (A, B). When theOperation key is pressed, the set
mixing volume is aspirated.

Dispensed liquid（A）
Liquid（B）[ 4 ] The combined liquid (A,B) will be mixed
by repeating the aspiration/dischargeof around 2/3 of the combined liquid forthe set number of mixes.
[ 5 ] Once the mixing process has been
completed, a buzzer will sound once.

[ 6 ] Discharge the liquid remaining in the tipby pressing theOperation key(when the blowout function ison ).
The mixed liquid will be completely
discharged and the dispensing andmixing operation completed.

When the operation is complete, thebuzzer will sound twice.
42-2.

Sequential Aspirating mode (SA)1 ) Selection and setting method for Sequential Aspirating mode
(Example of initial display)
Setting example starts below[ 1 ] Press theBack key.

[ 2 ] Select “MIX” using theUp/Down key.

[ 3 ] After holding down theUp key forabout 3 seconds, “SE” will start flashing.

[ 4 ] After pressing theEnter key, “SE” will
fully light up and “SA” will start flashing.

[ 5 ] In the “SA” display, press theEnter key.

[ 6 ] Using theUp/Down key, set thevolume of the liquid to be aspirated
first (A).
(In the example, 10μL is set)
[ 7 ] The volume to be aspirated first isdetermined by pressing theEnter key.

5

[ 8 ] Set the volume of the liquid to beaspirated the second time (B) using the
Up/Down key.
(In the example, 20μL is set)
[ 9 ] The volume to be aspirated second isdetermined by pressing theEnter key.
In a similar manner, set the further
required aspiration volumes in the correct
sequence.
For example, when 3 types of liquid are
to be aspirated, in order to not aspirate a
4th time, when “04” is flashing, press theEnter key when the set volume
appears as “---”.

□Except for the MPA -1200, MPA -10000 when aspirating, an air
gap will be aspirated between each liquid.
□Aspiration amount for each air gap
MPA -10

MPA -20

MPA -200

MPA -1200
MPA -10000

0.1μL

2.0μL

20μL

Does notaspirate air gaps

□If the total volume of all liquids and air gaps is greater than themaximum volume of the MPA pipette, it cannot be set.

62 ) Operation method for SA mode (when using MPA-200)[ 1 ] Put the end of the tip in the liquid tobe aspirated first (A), and aspirate bypressing the
Operation key.

Liquid first aspirated（A）[ 2 ] Remove the tip from the liquid andaspirate air by pressing theOperation key.
(Except for the MPA -1200)

Liquid（A）

Air gap[ 3 ] Put the end of the tip into the liquid to beaspirated second (B), and aspirate bypressing theOperation key.

Liquid aspirated first（A）
Liquid to be aspirated second（B）[ 4 ] Remove the tip from the liquid andaspirate air by pressing theOperation key.
(Except for the MPA -1200)

Liquid（A）
Liquid（B）

7

Air gap
Air gap[ 5 ] Put the end of the tip in the liquid to beaspirated third (C), and aspirate bypressing theOperation key.
Liquid aspirated 1st（A）
nd

Liquid aspirated 2 （B）
Liquid to be aspirated 3rd（C）

Liquid（A）
Liquid（B）
Liquid（C）

Air gap
Air gap

Once the set number of aspirations
has been completed, the total
aspirated volume is displayed.
(The volume of the air gaps is notincluded.)[ 6 ] Put the end of the tip into the vessel
and discharge the liquids (A,B,C) all
together by pressing theOperation key.

[ 7 ] Discharge the liquid remaining in
the tip by pressing theOperation key.
(when blowout function ison)

8

2-3.

Sequential Dispensing mode (Sd)1 ) Selection method for Sequential Dispensing mode
(Example of initial display)
Setting example starts below[ 1 ] Press theBack key.

[ 2 ] Select “MIX” by using thekey.

Up/Down[ 3 ] After holding down theUp key forabout 3 seconds, “SE” will start flashing.

[ 4 ] After pressing theEnter key, “SE” will
fully light up and “SA” will start flashing.

[ 5 ] “Sd” will be displayed flashing once
selected with the[ 6 ] Press thedisplay.

Up/Down key.

Enter key once in “Sd”

[ 7 ] Set the volume to be dispensed
first (A) using the
Up/Down key.
(In the example, 10μL is set)9

[ 8 ] The volume to be dispensed first (A) isdetermined by pressing theEnter key.

[ 9 ] Set the volume to be dispensed second(B) using the Up/Down key.
(In the example, 20μL is set)
[ 10 ] The volume to be dispensed second(B) is determined by pressing theEnter key.
In a similar manner, set the further
required dispensing volumes in thecorrect sequence.
For example, when 3 volumes of liquid
are to be dispensed, in order to notdispense a 4th time, when “04” isflashing, press the Enter key when
the set volume appears as “- - -”.

２ ) Operation method for Sequential Dispensing mode
[ 1 ] Put the end of the tip in the liquid
to be dispensed and press theOperation key to aspirate the liquid.

10[ 2 ] Put the end of the tip into the vessel forreceiving the dispensed liquid (1) andpress theOperation key todispense the first dispensing volume (A).

1st dispensed volume[ 3 ] Put the end of the tip into the next
vessel (2) and press theOperation key to dispense thesecond dispensing volume (B).
[ 4 ] Put the end of the tip into the next
vessel (3) and press theOperation key to dispense thethird dispensing volume (C).

Volume A Volume B Volume C

In a similar manner, dispense thefurther required dispensing volumes in
the correct sequence.
[ 5 ] After the liquids have been dispensed,“rE” is displayed. The remaining liquid
is discharged by pressing theOperation key as in reverse mode.

11