jpn.j.Phyco
L(Sorui)37:239‑
243，September20，1
989HiroshiKAWAI:FirstreportofPhαeosαccioncollinsiiFARLOw(Chrysophyceae，JSarcinochrysidales)from apan
KeylndexWordr ChりIsothyceae‑PhaeosaccioncoIlinsii‑
SarcinochウsidalesfBotany，Faculty01Science，HokkaidoUniversity，Sattoro，060JatanHiroshiKawaz;DetartmentoーAn algareferabletoP
haeosaccioncollinsii fixed in 3% glutaraldehyde in 0.
1M
FARLOW(Chrysophyceae，Sarcinochrysidales， costfixedin2% OS04inacodylatebu
ffer，pP
haeosaccionaceae) was collecte
din March 0na.
1M cacodylatebuffer，dehydrated i
1
987atIsoya，Hokkaido，facin
gt
ot
heSeaof acetoneser
ies and embedded inSpur
r'sjapan(leg.Mr.Y.Sumita). Itoccurredas epoxyrectionedwithaesin(SPURR 1969)，stu
ftonsubtidalrocksa
tadepthofabout4m
diamond knife， and stainedwithuranyll. Thet
hallu
s a
belowMeanLowWaterLeve
ce
tat
eandl
eadc
itrate. Observ
ation
swere
saccate，to20m minl
ength madeu
wasoliv
e‑brown，singH
itach
iH‑300TEMattheIn‑(Fig.1). I
tresembledyoungt
hallusofsome sacultyo
ftituteforAIgological Research，Fbrownalg
asuc
hasPunctar
iao
rPetaloniabut Sniv
ersity].
Thecience， Hokkaido U
wasdistingu
ishe
dbyt
hehollowhabit(Fig.2)， posteriorflagellumofthisalga showed t
hebrighter colour and so丘 texture. The greenishautofluor
escenceasseenin manycomposed phaeophyceanz
holdfastwass
mal
landdisc‑s
haped，oospores(KAWAI1988). Myofsev
eralcells(Fig.3). R
hizoidalfilaments specimensagreewellw
iththeoriginaldescrip‑were not observed. The thallus was thaeosaccioncol
・
ionandprev
iou
sreportsofPac‑ lpolystichous(Figs. 4and5)，becomings
insiiFARLOW(FARLOW1
882，MCLACHLANetal.
cat
eandmonostromaticw
ithincreasedsize 1971)，
andwitht
hespecimeno
ftl
山 speciesin(Fig.2). Thecellso
fthethallusshowedno PhycothecaBoreali‑Americana(COLLINSetal.
insur
fac
e 1morpholog
i
cald
i
ffer
ent
iat
ionsand，895)int
heg
ene
r
alhabit and t
he mor‑rr
ange
dinpacketso
f two tofour pview，at was identifiedashology. Accor
dingly，iacho
f Pcells. Thesecellsar
e6‑8x6‑9μm，ehaeosaccio
ncollinsii.
whichcont
aine
dap
ar
ietalchloroplas
twit
ha
Although P
haeosaccio
ncollinsiih
as beenprominentp
yrenoid(Figs.6and7). Ani
n‑ reportedfromv
ariou
slocalitiesi
ncold‑water，thisistheareasoftheNorth
Atlantic
Ocean
ner lamella was obser
v
e
dint
h
ep
yr
e
noidir
stre
por
tfort
hePacifi
c Ocean. Theallcell
softhethallus f(Fig.
6). Whenmature，
pecieswas firstdescr
ibedas abrown algaweretransfor
medintozoospores. Afterthe snclude
dinthePunctar
iaceae(FARLOWnlya gelatinou
s andireleaseofthezoospores，o1964)sug‑
membrane remained (Figs. 8 and 9). 1882). Later，PARKEandDIXON(ested that it be transferred to theZoospores were pyr
ifor
m and typically gupportedbyt
heflagellatedwithalongeranter
iorflagellum Chrysophyceae，whichwasstal.(1971)，CRAIGIE
tu
diesofMcLACHLANe
andas
horte
rposteriorone(Fig.10). They stal.(1971)，andCHENetal.(1974)ont
hel
ifecontainedachloroplastprov
idedwith an eistory，morphology，chemicalcomposit
ioneyespo
t and a pyrenoid (Figs.1
1 and 12)
. hin
estr
uctur
eo
ft
hes
pe
cies.
Tr
ansit
ionalhelice
swereobserv
edinthetran‑ andfCultures were started from zoospor
es，sitionalregiondistaltothebasalplate(Fig.
eleasedfromthefield‑collectedthalli，byt
heaterialwas r13)
. [ForTEMobserv
ations，mpipetting method. They were culturedinApartofthisworkwassupportedbyaG
rant‑
in‑
Aidndiameter，olystyrenepetri‑dishes，90m miforScienti
ficResearchfromtheM
in
istr
yo
fE
du
cation， pS
cienceandCultureofj
apan(No.63740387).
using PESI medium (TATEWAKI 1966).

2
40KAWAI，H.

Culturecondition
swere50C SD(shortday; Acknowledgments
08:16hLD)，5 C L D (long day; 16:8hLD)，.Y. SUMITA，Ocean
1amg
ratefultoM r
100C SD，lOoC L D，150C SD，150C LD，&S
ystemwor
ks
Co.，
Ltd，
forcollect‑
Research0020 C SDand20 C L D，underwhitefluores‑0r.
ingthespecimen.
1amalsogratefultoD125 C) or
cent light ofabout 28μMm‑s‑ (.McLachlan，NationalResearchCouncilof
10oC，150C，20oC). (Lux J
46μMm‑2s‑1 (Canadaforhi
scr
i
ti
c
a
lreadingandimproving
values were measured using a photocelli
l‑heEnglishoft
hemanuscript.
luminometer and convertedt
o quantumir‑ tradiance by thefollow
ingrelation: 2501ux2ニ 4.6μMm‑s一1.
) The zoospores became References
roundedandformedwallsaftersettlementon.C‑M.，McLACI霊LAN，J. andCRA阻 E，J.S.
the substratum. They germinated in a CHEN，L1
974. T
hefinestructur
eofthemar
inechrysophy‑ir
ect type of germination andunipolar，dceanalga Phae却'，osac町，
CZ
切
4o叩nc
印0必/'，/i
訂叩叩nIsSli.Can.J.Bot.5
2:
developedintoprostratebranchedfi.
laments
1621‑1624，
4pls.
(Fig.15) or directlyintoerectthalli(Fig. COLLIN
S，F.S.，
B
RI
DGEPO
R
T，1.H.andS
ETCHELL，W.A.
14). Erectthalliwerefi.rstuniseriate，then
1895. PhycothecaBoreali‑Americana. AcolIec‑tionofdriedspecimensofthealgaeofNorthl1s (Fig. 14) andformed longitudinal wa.Malden，MassachusettsAmerica.F
ascicle1
became saccate(Fig.16). Well‑developedCRAIGIE，J.S.， LEIGH， C.， CHEN， L.C‑M. anderectthalliattained a length of5mm. At.1971. Pigments，polysaccharides
McLACHLAN，J50C，
erectthallidevelopedi
nt
osaccatethallihaeosaccioncollinsii.
andp
hotosyntheti
cproductsofP
similartot
hep
lanti
nnature. However，Can.J.Bot.4
9:1067‑1074.
otesonNewEnglandalgae.
theyonlyformedfi.
lamentousthalliatl
OoC. FARLOW，W.G. 1882. NI.TorreyBot.Clu
b9:65‑
68.
Bul
At150C and20oC，
theydidnotgroww
ell.
KAWAI， H. 1
988. A flavin‑like autofluorescent
Maturethall
iformedzoospores at50C andlIumofgoldenandsubstanceintheposteriorflageffects ofl
OoC. There were no obvious ebrownalgae.J.Phycol.24:1
14‑117.
photoperiodonmorphogenesisormaturation McLACHLAN，J.，CHEN，L.C‑M.，EDELSTEIN，T. andCRAIGIE，J.S.1971.Observ
ationsonP
haeosaccionco/‑ofthethalli. Theresult
sofmycultureex‑linsiii
ncuIture.Can.J.Bot.4
9:563‑566，2pls.
perimentsagreewiththoseofMcLACHLANetPARKE，M.andDIXON，P.S.1
964. Arev
isedcheck‑listal. (1971) on the lifeh
i
stor
yp
atterninofB
ritishmarinealgae.J.mar.biol.A
ss.U.
K.4
4:
responset
othetemperature.
4
99‑
542.
SPURR，A.R.1
969. Alow‑viscosityepoxyresin‑
embed‑.
ding medium for electron microscopy. JUI
trastruct.Rec.2
6:3
1‑
43.
TA
TEWAKI， M. 1
96
6. Formation ofacrustoseosiphonsporophyt
ewithunilocularsporangiainS.
りtlomentar
ia.Phycologi
a6:62‑
66.

F邪 . 1‑
9. P
haeosacci・on印 llinsiiF
ARLOWinnatu
re. F
ig.1. Habi
tofthethallus(arrowheadshowsmaturepar
tofthe出 allus). F
ig.2. Cros
ss
ect
ionofthes
accatethallus(embeddedinSpurr'sepoxyresinandstainedw
ithtoluidineblue)
. F
ig.3
. B
asalpartofthethallus(asterisk)andyoungu
niser
iateerectf
ilamentouslIus
. F
ig.4
. Y
oungpolystichou
sfilament. F
ig.5
. Y
oungs
accatethallu
s. F
ig.6. TEMmicrographthalI(C，chloroplast;Nu，nu
cleus;P，pyrenoid;arrowheads，innerlamelIa). Fi
g7. Sur
faceo
fav
egetativ
ecelIus. Fig.8. S
ur
facev
iewo
fm
atur
ep
a口oft
hallus(arrowheadsshowempt
iedv
iewo
fv
egetativ
epar
toftha. Sur
facev
iewo
falmostemptiedt
hallusafterreleasesofzoospores.
area)
. Fi
g.9目

P
haeosaccio
ncolli
71si
inewt
oJ
apan

241

，HKAW
AI242

圃圃司ド m

50ドm ‑Figs.1
0ー 16. Phaeosaccio
ncolli7lsiiFARLO
Win natu
reand incultu
re. Fig.10. Releas
ed t
yp
ic
aly l
fagela
tedz
oos
pore
. Fig
.1
1. TEMmicrographofmat
u
repar
toft
het
hall
us(aster
is
ks
howsg
el
ati
nousmembrane，
andar
‑r
owhe
ads
howspyrenoid).Fig.1
2. TEMmicrographofaz
oo
spor
ebef
or
er
el
ease(arrowheads
howsbasalpar
t
andas
t
er
is
ks
howse
ye
spot)
. Fi
g.1
3. TEMmicrographofaAa
geluminl
ong
itu
dinalsection(arrow
offlagela，alhelices). F
ig.1
4. Young e
rec
tf
il
ame
ntousthalusins
howsbasalpl
ate，and arrowhead shows tran山 oncu
ltu
rewi
t
hou
tp
r
os
t
rat
efi
J
amen
ts(arr
ow s
hows1
印
0n
咋1
伊9l
山凶t
l
山
吋u
i
Young町
er
伐
e
αc
削
tf
i
lamen
toust
haluswi
t
hp
ros
tr
at
efi
lame
nts(arrowhead). Fig.J6. Saccat
ethallusinculturePhaeosaccioncolli叩， inewt
ojapan24j

川井浩史:日:本新産黄金色藻 Phaeosaccioncolli
悶 iiFARLOW (フクロコガネモ:新称)
北海道磯谷において採集された比較的大型の葉状体を形成する黄金色藻を P
haeosaccioncollinsiiFARLOW (サルシノタリシス目，フタロコガネモ科;新称)と同定した。藻体は袋状で 1層の細胞層からなり，細胞は表面観で6‑8x6‑9μmで， 1個のピレノイドを伴った色素体を含む。成熟すると藻体の全体の細胞がそれぞれ 1個の遊走子に変成する。遊走子は涙滴形で，典型的な褐藻型の側生する 2鞭毛， 1個の色素体と限点を有する。透過型電顕による観察の結果ピレノイドには内膜構造が，また鞭毛基部にはらせん構造が見られた。本種は北大西洋の冷水域の広い範囲から報告されているが太平洋沿岸からの報告はこれが初めてである。本種の遊走子を 5‑200C の長日，短日条件て培養した結果，直接型の発芽の後，分枝した糸状体を経て，直立体を生じた。直立体は初め単OoC で藻体は成長，成熟し自然藻体と同様の遊走列で，次いで縦の爾壁を生じ，袋状の藻体に発達した。 50C，l060札幌市北区北 10条西 8丁目子を生じた。 (北海道大学理学部植物学教室)