GC Technical Note

GT037
GL Sciences Inc.

Simple Concentration and Analysis of Fresh Flower
Fragrance Components - Using Sorptive Media MonoTrap

Preliminary processing procedure
One fresh flower (Mizusen)

Collection (passive)

In ambient air for 24hours

MonoTrap DCC18 x 5 sheets

GC Conditions

1000 μL of diethyl ether
5 minutes of ultrasonication

Solvent extraction

System: GC - MSColumn: InertCap Pure-WAX
0.25 mm I.D. x 30 m df = 0.25 µm

Col. Temp. : 70 ˚C - 4 ˚C /min - 220 ˚CCarrier Gas : He 90 kPa

With nitrogen purging
Concentrate to a few µL

Concentration

Injection: Split 1:10
250 ˚C

Detection : MS Scan (m/z 50-450)
Sample Size : 1 µL

GC/MS

1. ß-Linalool
2. Clorius
3. Benzyl Benzoate*Results of identification withstandard samples

Time (min)GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly.
We reserve the right to amend this information or data at any time and without any prior announcement.

GL Sciences, Inc. JapanGL Sciences B.V.

GL Sciences, Inc. USA

GL Sciences (ShangHai) Ltd.

22-1 Nishishinjuku 6-Chome
Shinjuku-ku, Tokyo,163-1130, Japan
Phone: +81-3-5323-6620
Fax: +81-3-5323-6621
Email: world@gls.co.jp
Web: www.glsciences.com

De Sleutel 95652 AS Eindhoven
The Netherlands
Phone: +31 (0)40 254 95 31
Email: info@glsciences.eu
Web: www.glsciences.eu

4733 Torrance Blvd. Suite 255
Torrance, CA 90503
Phone: 310-265-4424
Fax:310-265-4425
Email: info@glsciencesinc.com
Web: www.glsciencesinc.com

Tower B, Room 2003,
Far East International Plaza,NO,317 Xianxia Road,
Changning District.
Shanghai, China P.C. 200032
Phone: +86 (0)21-6278-2272
Email: contact@glsciences.com.cn
Web: www.glsciences.com.cn

International Distributors
Visit our Website at www.glsciences.com/distributors

GL Sciences GC Technical Note

Sample name and retention index
Component
RI
1. Methanol
362
2. Ethanol
439
3. Acetonitrile486
4. Acetone488
5. 2-Propanol(Isopropyl alcohol) 488
6. n-Pentane
500
7. Diethyl ether
504
8. Ethyl formate
515
9. 1,1-Dimethoxymethane515
10. 1,1-Dichloroethylene
518
11. Methyl acetate524
12. Dichloromethane530
13. 1-Propanol
550
14. trans-1,2-Dichloroethylene 560
15. tert-Butyl methyl ether
562
16. Nitromethane564
17. 2-Butanone(MEK)597
18. 2-Butanol
600
19. n-Hexane
600
20. Diisopropyl ether
600
21. cis-1,2-Dichloroethylene
611
22. Ethyl acetate611
23. Chloroform
619

Component
RI
24. 2-Methyl-1-propanol
624(Isobutyl alcohol)
25. Tetrahydrofuran
630
26. 2-Methoxyethanol
630(Methyl cellosolve)
27. 1,2-Dimethoxyethane650
28. 1,1,1-Trichloroethane650
29. 1,2-Dichloroethane650
30. 2,2-Dimethoxypropane 650
31. Methyl isopropyl ketone 658
32. Isopropyl acetate658
33. 1-Butanol
658
34. Benzene
666
35. Carbon tetrachloride
666
36. Cyclohexane666
37. 2-Methyltetrahydrofuran 672
38. 2,2,4-Trimethylpentane 690
39. n-Heptane
700
40. Trichloroethylene
704
41. 2-Ethoxyethanol712
42. n-Propyl acetate712
43. 1,4-Dioxane
712
44. Methylcyclohexane
731

Component
RI
45. 3-Methyl-1-butanol
731(Isoamyl alcohol)
46. 4-Methyl-2-pentanone(MIBK) 739
47. Pyridine
748
48. 1-Pentanol(Amyl alcohol)
764
49. Isobutyl acetate772
50. Toluene774
51. N,N-Dimethylformamide
781
52. 2-Hexanone(MBK)789
53. Propionaldehyde diethyl acetal 811
54. n-Butyl acetate
811
55. Dimethyl sulfoxide(DMSO)
834
56. Chlorobenzene
858
57. N,N-Dimethylacetamide
870
58. Ethylbenzene
870
59. p-Xylene879
60. m-Xylene879
61. o-Xylene
905
62. Anisole
926
63. Cumene936
64. N-methyl-2-pyrrolidone
1050
65. 1,2,3,4-Tetrahydronaphthalene1194
66. Sulfolane
1232

Xylene used is a mixture of m Xylene, p Xylene, o Xylene, and Ethylbenzene.
Retention indices are...
This value is based on the carbon number of straight-chain alkanes and is calculated using theretention time of each component and hydrocarbon.
In this application, a temperature-rise analysis was made . The formula is shown below.
TR= retention time of the target component
T R(Z) = retention time of straight-chain alkanes that precede theTR - tR(Z)Retention I = 100 ×+ 100 ×Z components of interestTR(Z+1) = retention time of straight-chain alkanes emerging after theTR(Z+1)
tR(Z)
index
component of interest.
Z= number of carbon in the straight-chain alkane with retention
time tR(Z)GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly.
We reserve the right to amend this information or data at any time and without any prior announcement.

GL Sciences, Inc. JapanGL Sciences B.V.

GL Sciences, Inc. USA

GL Sciences (ShangHai) Ltd.

22-1 Nishishinjuku 6-Chome
Shinjuku-ku, Tokyo,163-1130, Japan
Phone: +81-3-5323-6620
Fax: +81-3-5323-6621
Email: world@gls.co.jp
Web: www.glsciences.com

De Sleutel 95652 AS Eindhoven
The Netherlands
Phone: +31 (0)40 254 95 31
Email: info@glsciences.eu
Web: www.glsciences.eu

4733 Torrance Blvd. Suite 255
Torrance, CA 90503
Phone: 310-265-4424
Fax:310-265-4425
Email: info@glsciencesinc.com
Web: www.glsciencesinc.com

Tower B, Room 2003,
Far East International Plaza,NO,317 Xianxia Road,
Changning District.
Shanghai, China P.C. 200032
Phone: +86 (0)21-6278-2272
Email: contact@glsciences.com.cn
Web: www.glsciences.com.cn

International Distributors
Visit our Website at www.glsciences.com/distributors