GC Technical Note

Analysis of Flavor Components in Japanese Sake- Using a Headspace Autosampler

GT051
GL Sciences Inc.

n-Propyl alcohol, Ethyl acetate, Isobutyl alcohol, Isoamyl alcohol, Isoamyl acetate, and Ethyl caproate
are all known as flavor constituents in Japanese sake and are particularly abundant among several flavor
constituents. In this application two types of Japanese sake were analyzed with a headspace
autosampler using a standard addition method to determine the components detailed above.

Example: Measurement of sample

300000Japanesesake ②01020010Time (min)Conditions
SystemColumnCol.Temp.
Carrier Gas
Detection

20Time (min)HS Conditions: GC - FID - HS (Tekmar HT3)
: AQUATIC
0.32 mm I.D. x 60 m df = 1.40 µm: 40 ˚C (5 min) - 5 ˚C/min - 100 ˚C- 10 ˚C/min - 200 ˚C (13 min)
: He 100 kPa: FID Range 10^0220 ˚C

Sample.Temp.
Sample Equil. Time
Mixing Time
Mixing Stabilize Time
Loop Size
Injection: 50 ˚C: 15 min: 10 min: 5 min: 0.5 mL
: Direct

Ethyl caproate

Isoamyl acetate

Isoamyl alcohol

Isobutyl alcohol

Ethyl acetate

n-Propyl alcohol

2000001000000Ethyl caproate

Isoamyl acetate

Isoamyl alcohol

Isobutyl alcohol

0n-Propyl alcohol

100000200000Ethyl acetate

300000Japanesesake ①

GL Sciences GC Technical Note

Calibration curves

Japanese sake (1) standard addition methodn -Propyl alcohol

Ethyl acetate

8000006000000

7000005000000

6000004000000

5000003000000

4000003000002000000

y = 4201 x + 124401
R2 = 0.9992

20000010000000-50

y = 27649 x + 1099006
R2 = 0.9989

1000000

050100150
Concentration (mg/L)

200-50050100150
Concentration (mg/L)

Isobutyl alcohol

Isoamyl alcohol

1400000

1600000

1200000

1400000

1000000

1200000
1000000

800000800000600000600000400000y = 6892 x + 174065
R2 = 0.9976

2000004000002000000-50

y = 6963 x + 385054
R2 = 0.9981

0050
100
150
Concentration (mg/L)

200-100-50

050
100Concentration (mg/L)

Isoamyl acetate
30000035000025000030000050000

200250000200000150000100000150

Ethyl caproate

200000150000y = 53345 x + 37182
R2 = 0.9936

100000y = 61994 x + 38583
R2 = 0.9937

50000

0-2

200

002
Concentration (mg/L)

46-202Concentration (mg/L)

46

GL Sciences GC Technical Note

Calibration curves
Japanese sake (2) standard addition method
Ethyl acetaten -Propyl alcohol
8000003500000

7000006000003000000
2500000

5000004000002000000
1500000

300000200000-100

1000000

y = 3608 x + 188744
R2 = 0.9964

1000000-50050100Concentration (mg/L)

y = 27538 x + 583209
R2 = 0.9942

5000000150

200-50050
Concentration (mg/L)

100

Isoamyl alcohol

Isobutyl alcohol
1400000

2000000

1200000
1500000

1000000
8000001000000

600000400000y = 6247 x + 205948
R2 = 0.9991

2000000-50y = 5925 x + 419157
R2 = 0.9994

5000000050
100
Concentration (mg/L)

150

200-1000Isoamyl acetate
180000160000140000120000100000
80000
60000
40000
20000
0-10100
200
Concentration (mg/L)

300

Ethyl caproate
1200000
1000000
800000600000y = 42670 x + 24853
R2 = 0.9979

400000y = 56229 x + 226726
R2 = 0.9997

200000012Concentration (mg/L)

34-5

0510
Concentration (mg/L)

1520

GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly.
We reserve the right to amend this information or data at any time and without any prior announcement.

GL Sciences, Inc. JapanGL Sciences B.V.

GL Sciences, Inc. USA

GL Sciences (ShangHai) Ltd.

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