MonoSelect C18for HTS
Monolithic silica-based column forHigh-Throughput Screening (HTS)
designed for LC and LC/MSWhy choose MonoSelect C18 for HTS?
MonoSelect C18 for HTS is based on monolithic silica technology. The very low flow resistance and theexcellent separation performances make it suitable for High-Throughput Screenings (HTS). Due to its metalfree hardware, sharp peaks can be obtained also when analyzing metal chelators. The particular structure ofMonoSelect C18 for HTS prevents from clogging and delivers high resistance.

 Suitable for High-Throughput
Screenings (HTS) Suppression of adsorption by metalchelation
 High durability

Monolithic Silica
GL Sciences’ monolithic silica consists of precisely
controlled µm-sized co-continuous silica skeletons andthrough-pores. The high external porosity of thisstructure results in a lower pressure than that of particlepacked columns. Due to the mesopores in the silica
skeletons, the surface areas of monolithic silica andparticle-packed columns are comparable.

Silica skeleton

through-pore

Specifications
・Support Material：High Purity Monolithic Silica Gel
・Bonded Phase：Octadecyl
・Max. Operating Pressure ： 40 MPa
・End-capping： Yes・pH Range： 2-7.5
・Max. Operating Temperature: ： 70℃
・Carbon Loading：7%

Why choose MonoSelect C18 for HTS?
MonoSelect C18 for HTS allows for equal or even better separation than C18 3 µm silica particle packed columns, and it
can be used at both low or high flow rates. The back pressure is only 1/6 of the pressure generated by 1.7 µm silica
particle packed columns, making it suitable for high throughput analysis.
0.02
HETP

0.015

MonoSelect C18 for HTS

0.01

Column A (C18, fully他社カラムAporous 3 µm particles)
全多孔性C18,3µm0.005

全多孔性C18,1.7µm
他社カラムCColumn C (C18, fully porous
1.7 µm particles)

0012345678910111213Pressure (Mpa)

Linear Velocity (mm/sec)

60
50
403020100

The column with smaller particles size than 2 µm
shows very high back pressure012345678910 11 12 13

Linear Velocity (mm/sec)
Contact us for more detailed analytical conditions.

Suitable elution time for high-throughput analysismAU500

The faster elution of MonoSelect C18 for HTS compared to other C18 columns and its improved sensitivity make it thebest choice for high-throughput analysis.

Column A
12

Theoretical Plates (peak No.10): 1614

35689234Time (min)Column100471mAU

500

0567Column B
4

Theoretical Plates (peak No.10): 1640
56 7891001237456 83211234Time (min)5679MonoSelect C18 for HTS
Theoretical Plates (peak No.10): 2460

100mAU

5000Eluent
Flow Rate
Col. Temp.
DetectionInjjection. Vol.
Sample：MonoSelect C18 for HTS (20 x 2.1 mm I.D.)
Column A (3 µm, 20 x 2.1 mm I.D.)
Column B (3 µm, 20 x 2.1 mm I.D.)：CH3CN/H2O = 50/50,v/v：0.3 mL/min：40℃：UV 254 nm：1.0 µL：1) Thiourea
2) Acetanilide
3) Acetophenone
4) Propiophenone
5) Butyurophenone
6) Benzophenone
7) Naphtalene8) Hexanophenone
9) Heptanophenone
10) Octanophenone

01234
Time (min)567
Contact us for more detailed analytical conditions.

MonoSelect C18 for HTS
Up to the limits of the fastest equilibration
The equilibration time plays an important role in the result repeatability in gradient analysis. MonoSelect C18 for HTS
enables faster equilibration compared to packed columns of the same dimensions, which is desirable for rapid analysis.
MonoSelect C18 for HTS

Column A

Column B(20 x 2.1 mm I.D.)(3 µm, 20 x 2.1 mm I.D.)(3 µm, 20 x 2.1 mm I.D.)

Measurement
afterequlibration① 0 minConditions
Eluent② 1 minTime (min)
0.00
1.00
1.60
2.20
2.21

Equilibration③ 2 min
0Time (min)1.001.0Time (min)A (%)
95950095

B (%)
55100
1005Flow Rate：0.3 mL/min
Col. Temp.
：40℃
Detection：UV 215 nm
Injection. Vol. ：1.0 µL
Sample：1. Methamidophos
2. Acephate

Equilibration④ 3 min：A) H2OB) CH3CN

Comparison of mobile
phase B (%)

Equilibration
100⑤ 4 min
0Time (min)1.0Contact us for more detailed analytical conditions.

Equilibration Time①②③⑤④

50

1.0 1.6 2.2Time (min)Superior repeatability during continuous injections
Below data was obtained by performing a continuous injection test on a sample containing 11 compounds andcomparing the repeatability of the peak area obtained by each column. The superior repeatability of MonoSelect C18for HTS is attributed to its fast equilibration.
EluentFlow Rate
Col. Temp.
Detection
Injection Vol.

02.0

4.0Time (min)6.0

8.0：A) 0.1 % HCOOH in H2O：0.6 mL/min：40℃：LC/MS/MS：1 µLB) 0.1 % HCOOH in CH3CN

Time (min)
0.00
1.00

A (%)
95
10B (%)
590① Acetaminophen⑦ Trimipramine② Pindolol⑧ Imipramine③ Caffeine⑨ Amitriptyline④ Diphenhydramine⑩ Chlorpromazine⑤ Doxepin⑪ Clomipramine⑥ Desipramine

Time of one analysis cycle:
1 min Analysis Time + 1 min Sample Injection Time (the stabilization is performed during this time) = 2 min
①②③

④

⑤

⑥

⑦⑧

⑨⑩

⑪Ave.

MonoSelect C18 for HTS

2.21%

2.76%

8.89%

4.64%

6.51%

8.39%

3.22%

4.97%

3.17%

4.00%

4.66%

4.86%

Column A

16.0%

13.1%

13.7%

11.0%

7.31%

7.25%

5.80%

6.81%

6.64%

4.88%

7.56%

9.10%

Column B

6.61%

6.45%

9.02%

3.36%

6.49%

7.86%

7.44%7.62%

6.98%

6.80%

8.96%

7.05%

Contact us for more detailed analytical conditions.

Stainless steel and glass double structure to prevent absorption
MonoSelect C18 for HTS structure includes an external stainless hardware and an internal glass tube. The structure does
not contain any sintered metal frit, which could cause adsorption of metal chelators. This delivers outstanding sensitivity
for such analytes.

MonoSelect C18 for HTS cartridge

Cartridge internal structure

Stainless steel tube
Solvent resistant resin
Glass tube
Monolithic silica【Analysis of Fumonisin B1】Fumonisin B1 is a type of mycotoxin, and the several carboxyl groups of this compound could be a metal chelators. WithMonoSelect C18 for HTS, it is possible to obtain a sharp peak even when analyzing this kind of compound.

ColumnEluent

Fumonisin B101.0

2.0
3.0Time (min)4.0：MonoSelect C18 for HTS (20 x2.1 mm I.D.)
A) 0.1% HCOOH in H2OB) CH3CN
Time (min)A (%)
Flow Rate ：0.3 mL/min
0.00
80
3.00
5Col. Temp. ：40℃
3.0180Detection ： LC/MS/MS
5.0080Sample： Fumonisin B1B (%)
2095
20205.0【Analysis of ATP】
ATP contains 3 phosphate groups. Also in this case, good peak shape can be obtained with MonoSelect C18 for HTS.
1ColumnEluent

20Flow Rate
Col. Temp.
Detection
Sample3

1.0：MonoSelect C18 for HTS (2.1 x 20 mm)
A) 5 mM DBAA in H2OB) 5 mM DBAA in CH3OH
Time (min)：0.6 mL/min
0.00
：40℃
0.10：UV 280 nm
0.50：1. AMP
1.00
2. ADP
1.01
3. ATP
1.60

2.0Time (min)Contact us for more detailed analytical conditions.

A (%)

B (%)

1000802070304060

1090

1090

1.61

10002.00

1000MonoSelect C18 for HTS
Analysis of proteins
Peaks of macromolecules tend to be distorted with columns packed with particles having small pores. On the other hand,
MonoSelect for HTS yields sharp peaks of macromolecules due to the mesopores in the monolithic skeletons.

3.00Time (min)Column ：MonoSelect C18 for HTS (20 x 2.1 mm I.D.)
Column A(3 µm, 20 x 2.1 mm I.D.)
Column B (3 µm, 20 x 2.1 mm I.D.)
Eluent ：A) 0.1% TFA in H2OTimeB) 0.1% TFA in CH3CN(min)
0.00
3.00

60000
0 Time (min)3.003.060000

0mAUmAU3.003.00 Time (min)

60000

00mAU

600000mAU

60000

3.060000mAU0 Time (min)0Cytochrom c
MW：12,000

Column B

Column A

0Ribonuclease A
MW：13,000

mAU

MonoSelect C18 for HTS

Time (min)Flow Rate
Col. Temp.
Detection
Inj. Vol.

Time (min)：0.3 mL/min：60℃：UV 230 nm：5.0 µLA (%) B (%)
80602040Contact us for more detailed analytical conditions.

Superior DurabilityWhen analyzing compounds contained in physiological samples, proteins must be removed before the analysis in order
to avoid column clogging caused by the proteins themselves. However, it is difficult to completely remove all theproteins and this generally leads to the column deterioration over time. The large through-pores of MonoSelect C18 for
HTS avoids accumulation of interfering compounds in biological samples. This results in longer repeatability over timecompared to particle-packed columns.
120.0%● MonoSelect C18 for HTS (2.1 x 20 mm)● C18 Column A (3 µm, 2.1 x 20 mm)

Theoretical Plates (%)

100.0%
EluentFlow Rate
Col. Temp.
Inj. Vol.
Sample

80.0%

60.0%

Theoretical plates after 2000 injections
MonoSelect C18 for HTS ：100.7 %： 65.6 %C18 Column A

40.0%：CH3CN/H2O = 50/50,v/v：0.4 mL/min
：40℃
：2 μL
：Naphthalene

250 µL Control serum
Add CH3CN 750 µL

20.0%

Centrifuge at 10,000 xg for 5 min
0.0%
0200

400

600

800

1000

1200

No. of Injections

1400

1600

1800

2000

Inject 2 µL of the supernatantContact us for more detailed analytical conditions.

Products

MonoSelect C18 for HTS Holder Cartridge Kits
Description

Specifications

Cat.No.

10mm Holder Cartridge Kit

2.1 mm I.D. x 10 mm cartridge 1PC
Holder for 10mm cartridge 1PC

5020-10810

20mm Holder Cartridge Kit

2.1 mm I.D. x 20 mm cartridge 1PC
Holder for 20mm cartridge 1PC

5020-10811* The end- fitting is UP type (Parker style).

10mm Holder Kit

20mm Holder Kit

MonoSelect C18 for HTS Cartridges

Description

I.D. (mm)MonoSelect C18 for HTS
Cartridge

Length (mm)Cat.No.

Qty.

105020-10812

1PC

205020-10813

1PC

Length of the Cartridge
Applicable (mm)Cat.No.

Qty.

10mm

5020-10814

1PC

20mm

5020-10815

1PC

2.1

MonoSelect C18 for HTS Holder

Description

MonoSelect C18 for HTS Holder* The end- fitting is UP type (Parker style).

GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly. We reserve theright to amend this information or data at any time and without any prior announcement.

GL Sciences, Inc. JapanGL Sciences B.V.

GL Sciences (ShangHai) Ltd.

GL Sciences, Inc. USA

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