GC Technical Note

Simple Concentration and Analysis of Spice Flavor
Components - Using Sorptive Media MonoTrap

GT041
GL Sciences Inc.

In this application note, MonoTrap RCC18 (with activated carbon) was used to simply concentrate andanalyze the flavor components of spices.

Preliminary processing procedure
Spice species

Appropriate amount of each thyme;
black pepper, vanilla beans, cinnamon

GC Conditions
Collection (HS)MonoTrap RCC18 × 1-2

System: GC - MSColumn: InertCap Pure-WAX
0.25 mm I.D. x 30 m df = 0.25 µm

Col. Temp. : 40 ˚C(5 min) - 5 ˚C /min - 250 ˚CSolvent extraction

200 µL of ethanol
5 minutes ofultrasonication

Carrier Gas : He 1 mL/minInjection: Split 1:20250 ˚CDetection : MS Scan (m/z : 35-600)
Sample Size: 1 µL

GC/MSTime

CinnamonBlack pepper
Vanilla beans

Image of collection vials

Time (min)1. Copaene
2. β-Linalool
3. 1,7,7-Trimethylbicyclo[2.2.1]hept-2-yl acetate
4. 4,11,11-Trimethyl-8-methylenebicyclo[7.2.0]undec-4-ene
5. p-Menth-1-en-4-ol
6. Alloaromadendrene
7. Borneol
8. 4,7,7-Trimethylbicyclo[4.1.0]heptan-3-ol

9. γ-Muurolene
10. 3,7-Octadiene-2,6-diol, 2,6-dimethyl11. Caryophyllene oxide
12. Spathulenol
13. 2,6-Dimethyl-1,7-octadiene-3,6-diol
14. Unknown
15. Tau.-Cadinol
16. Carvacrol*According to the library search results

GL Sciences GC Technical Note

Black pepper

1. 4(10)-Thujene
2. 3-Carene
3. β-Myrcene
4. D-Limonene
5. o-Cymene
6. Copaene
7. Caryophyllene
8. α-Caryophyllene
9. Eudesma-4(14),11-diene
10. α-Selinene
11. Caryophyllene oxide*According to library search results

Time (min)Vanilla beans

1. Acetic acid
2. Butanediol
3. Butanediol
4. Vanillin*According to library search results

Time (min)GL Sciences GC Technical NoteCinnamon
1. Cycloisosativene
2. α-Cubebene
3. Sativen
4. Sativen
5. β-Elemene
6. γ-Muurolene
7. Eudesma-4(14),11-diene
8. β-Chamigrene
9. α-Muurolene
10. δ-Cadinene
11. Calamenene
12. α-Calacorene
13. Cinnamaldehyde
14. 3-Methyl-7,8-dihydroquinolin-5(6H)-one
15. Cedr-8-ene
16. Murolan-3,9(11)-diene-10-peroxy
17. α-Cadinol
18. Cadalene
19. CoumarinTime (min)*According to library search results

Initial use of MonoTrap…
Introduction to the convenient MonoTrap Start UP-KITDescription①②③④⑤⑥⑦⑧⑨MT HolderMT Stand
MT Extract Cup with Vial (20 mL)
Clean Pin Hole Septum with vial (40mL)
200 μL glass insert (flat bottom)
MonoTrap DCC18
MonoTrap RCC18
MonoTrap DSC18
MonoTrap RSC18Qty.
51554020202020①②③④⑥⑦⑤⑧⑨GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly.
We reserve the right to amend this information or data at any time and without any prior announcement.

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