pTAKN-2 vector Sequence (2739 bp)
The PCR product can be inserted at base 473(*).

There are T-overhangs at both 3’-ends. The

T-overhangs are not shown in this sequence.
151
101
151
201
251
301
351
401
451
501
551
601
651
701
751
801
851
901
951
1001
1051
1101
1151
1201
1251
1301
1351
1401
1451
1501
1551
1601
1651
1701
1751
1801
1851
1901
1951
2001
2051
2101
2151
2201
2251
2301
2351

TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCGGAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCGTCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATGCGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA
CCGCACAGAT GCGTAAGGAG AAAATATTAC TACAGGCGCC ATTCGCCATTCAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTATTACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTAACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAGCTAGTGTAATAC GACTCACTAT AGGGCGCGGC CGCAGAATTC GAGCTCGGTACCCGGGATCT CGAGGCCAGA TC*ATTGTGGATCCGCTCTAG AGTCGACCTGCAGGCATGCA AGCTTGCGGC CGCGTATTCT ATAGTGTCAC CTAAATAGCATGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTCACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGGTGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCGCTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAACGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCTCACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTCACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGAAAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGCCGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACAAAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGATACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGACCCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGGCGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTTCGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTGCGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACTTATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTATGTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACACTAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCGGAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGCGGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATCTCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACGAAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTCACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTATATATGTTTTA ATTAGAAAAA CTCATCGAGC ATCAAATGAA ACTGCAATTTATTCATATCA GGATTATCAA TACCATATTT TTGAAAAAGC CGTTTCTGTAATGAAGGAGA AAACTCACCG AGGCAGTTCC ATAGGATGGC AAGATCCTGGTATCGGTCTG CGATTCCGAC TCGTCCAACA TCAATACAAC CTATTAATTTCCCCTCGTCA AAAATAAGGT TATCAAGTGA GAAATCACCA TGAGTGACGACTGAATCCGG TGAGAATGGC AATAGCTTAT GCATTTCTTT CCAGACTTGTTCAACAGGCC AGCCATTACG CTCGTCATCA AAATCACTCG CATCAACCAAACCGTTATTC ATTCGTGATT GCGCCTGAGC GAGACGAAAT ACGCGATCGCTGTTAAAAGG ACAATTACAA ACAGGAATCG AATGCAACCG GCGCAGGAACACTGCCAGCG CATCAACAAT ATTTTCACCT GAATCAGGAT ATTCTTCTAATACCTGGAAT GCTGTTTTCC CTGGGATCGC AGTGGTGAGT AACCATGCATCATCAGGAGT ACGGATAAAA TGCTTGATGG TCGGAAGAGG CATAAATTCCGTCAGCCAGT TTAGTCTGAC CATCTCATCT GTAACATCAT TGGCAACGCTACCTTTGCCA TGTTTCAGAA ACAACTCTGG CGCATCGGGC TTCCCATACA

2401245125012551260126512701

ATCGATAGAT TGTCGCACCT GATTGCCCGA CATTATCGCG AGCCCATTTATACCCATATA AATCAGCATC CATGTTGGAA TTTAATCGCG GCCTTGAGCAAGACGTTTCC CGTTGAATAT GGCTCATAAC ACCCCTTGTA TTACTGTTTATGTAAGCAGA CATAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATACATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATTTCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACATTAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTC