pTAC-2 vector Sequence (2786 bp)
The PCR product can be inserted at base 473(*).

There are T-overhangs at both 3’-ends. The

T-overhangs are not shown in this sequence.
1
51
101
151
201
251
301
351
401
451
501
551
601
651
701
751
801
851
901
951
1001
1051
1101
1151
1201
1251
1301
1351
1401
1451
1501
1551
1601
1651
1701
1751
1801
1851
1901
1951
2001
2051
2101
2151
2201
2251
2301
2351

TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCGGAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG
TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATGCGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA
CCGCACAGAT GCGTAAGGAG AAAATATTAC TACAGGCGCC ATTCGCCATT
CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTATTACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAGCT
AGTGTAATAC GACTCACTAT AGGGCGCGGC CGCAGAATTC GAGCTCGGTA
CCCGGGATCT CGAGGCCAGA TC*ATTGTGGATCCGCTCTAG AGTCGACCTG
CAGGCATGCA AGCTTGCGGC CGCGTATTCT ATAGTGTCAC CTAAATAGCATGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTCACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG
TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG
CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAACGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT
CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTCACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGAAAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGATACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT
CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACTTATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTATGTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACACTAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCGGAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC
GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATCTCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTATATATGTTTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGCTGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAATAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG
TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAACGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTATCACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCCGTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGAATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATAATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT

2401
2451
2501
2551
2601
2651
2701
2751

TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTCGATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCACCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAGGGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCAATATTATTAT AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATATTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCCCCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA
CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTC