MonoSelect RP‐mAb

・ Intact mAb
・ Protein Analysis
・ Antibody‐Drug Conjugate (ADC)・ Subunit

Specifications
Support Material： Highly pure monolithic silica

Through‐pore Size

： 1 μmMesopore Size

： 60 nmMax. Operating Temp. ： 80 ℃

◎ Superiority of Monolithic Silica
1 µm Through‐pore

Monolithic silica consists of co‐continuous through‐pores and skeletons which have mesopores. The
large surface area and high permeability of thisstructure enables “strong retentivity and low
Monolithic Silica gel

60 nm Mesoporepressure”.

◎ 60 nm Pores for mAb AnalysisRepresentative chromatograms of protein analysis
MonoSelect RP‐mAb

Silica Particles

Broad peaks are obtained with conventional HPLC
columns when the analytes are huge proteinslarger than 10 nm such as antibodies. ”Sharp peaksof mAb” can be obtained with MonoSelect RP‐mAb
because the mesopores are designed to be 60 nm,
which is suitable for mAb analysis.

Applicability to Rapid Analysis Needed for Multisample Analysis
Rapid analysis with a high flow rate is possible with MonoSelect RP‐mAb because of “high permeability” of monolithic silica.

Flow Rate 1 mL/min
Initial pressure 6.5 MPa

Flow Rate 0.3 mL/min
Initial pressure 1.7 MPa

Increasing
flow rate

Distorted peaks are often obtained in protein analysis especially with low concentration samples. 60 nm mesopores andthe properly inertized surface yield sharp peaks even with low concentration samples.

Example of Low Concentration Analysis (1.4 mg/mL)
Analyte: Mab1 (Purified with MonoSpin ProA)

Peak Height

Column Pressure25136

4.5 MPa

Not GoodGood113250

10.5 MPa

GoodNot Good135451

1.7 MPa

Low Pressure but bad peak shape0.00Volts

0.10

Waters Column(Silica Gel)

0.0

1.0

2.0

3.0
Time (min)

4.0

5.0

6.0

Good peak shape but high pressure(unavailable for rapid analysis)

0.00

Volts

0.10

Agilent Column(Polymer)

0.0

1.0

2.0

3.0
Time (min)

4.0

5.0

6.0

0.10

MonoSelect RP‐mAb

Volts

Good peak shape ⇒ Best choice for low

0.00

concentration samples

0.0

1.0

2.0

3.0
Time (min)

4.0

5.0

Low Pressure ⇒ Available for rapid analysis

6.0

Available for Subunit Analysis
MonoSelect RP‐mAb is also applicable to subunit analysis as well as analysis of unreduced antibodies.
Before Reduction

After Reduction
H chain
重鎖軽鎖0.10

Volts

0.10

Eluent: A) 0.075 % HCOOH+ 0.025 % CF3COOH in H2OB) 0.075 % HCOOH+ 0.025 % CF3COOH in CH3CN
A/B = 80/20‐(5min)‐40/60

0.00

Oven Temp. : 80 ℃
0.00

Volts

L chainConditions

0.0

1.0

2.0
Time (min)

3.0

0.0

1.0

2.0
Time (min)3.0

Specifications
Base Material： Highly pure monolithic silica

Through‐pore size： 1 μm

Carbon Loading： 2.0 %Column Configuration : Cartridge Type： 80 ℃
Max. Temp.

Functional Group ： Phenyl Group

Mesopore size

： 60 nmI.D.

： 2.1 mmMax. Pressure： 40 MPa (400 bar)Surface Area

： 50 m2/gLength： 20 mm

pH Range： 2 ‐ 7.5

Description

Qty.

Cat.No.

MonoSelect RP‐mAb Holder Set

1 set

5020‐10818

MonoSelect RP‐mAb Cartridge

1 pc

5020‐10819

MonoSelect Cartridge Holder for 20 mm

1 pc

5020‐10815

Note) End‐fitting is UP (Parker type).

Related Products: MonoSpin ProA, ProG
The surface of monolithic silica functionalized with affinity ligands forantibody purification enables rapid purification.
Line up： Spin Column Type, 96 Well Plate TypeFeatures
 2 types of antibody affinity ligands: Protein A & Protein G Reduced risks of denaturation of antibodies due to short exposure to acid during elution
Description
MonoSpin ProA Column Type
MonoSpin ProG Column Type
MonoSpin ProA 96 Well Plate type
MonoSpin ProG 96 Well Plate type
MonoSpin L ProA
MonoSpin L ProG
MonoSpin ProA/G Buffer Kit[keep cooling][keep cooling][keep cooling][keep cooling][keep cooling][keep cooling][keep cooling]Qty.
10 pcs10 pcs
1 pc
1 pc
4 pcs
4 pcs
−Cat.No.
7510‐11310
7510‐11311
7510‐11312
7510‐11313
7510‐11314
7510‐11315
7510‐11316

Note 1) Each product includes chemicals for purification.
Note 2) GL‐SPE mini vacuum manifolds are recommended for vacuum filtration of 96 well plates. Contact us for more details.
Note 3) L type products include a centrifuge adapter. Centrifuge tubes are not included.

GL Sciences disclaims any and all responsibility for any injury or damage which may be caused by this data directly or indirectly. We reserve theright to amend this information or data at any time and without any prior announcement.

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